LOCUS       DAZPAB010000001        78102 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZPAB010000001 DAZPAB010000000
VERSION     DAZPAB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 78102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78102
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.001"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="ACHUPG_000001"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="ACHUPG_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR0859801.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2667)
                     /locus_tag="ACHUPG_000002"
     CDS             complement(1672..2667)
                     /locus_tag="ACHUPG_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0859802.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="ACHUPG_000003"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="ACHUPG_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHUPG_000004"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHUPG_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR0859803.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHUPG_000005"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHUPG_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR0859804.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHUPG_000006"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHUPG_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR0859805.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="ACHUPG_000007"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="ACHUPG_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR0859806.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="ACHUPG_000008"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="ACHUPG_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR0859807.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACHUPG_000009"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACHUPG_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR0859808.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACHUPG_000010"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACHUPG_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR0859809.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACHUPG_000011"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACHUPG_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR0859810.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACHUPG_000012"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACHUPG_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR0859811.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="ACHUPG_000013"
     CDS             complement(17699..18322)
                     /locus_tag="ACHUPG_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR0859812.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /gene="ypdI"
                     /locus_tag="ACHUPG_000014"
     CDS             18619..18894
                     /gene="ypdI"
                     /locus_tag="ACHUPG_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFR0859813.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="ACHUPG_000015"
     CDS             complement(18971..19213)
                     /locus_tag="ACHUPG_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR0859814.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="ACHUPG_000016"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="ACHUPG_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR0859815.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="ACHUPG_000017"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="ACHUPG_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR0859816.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="ACHUPG_000018"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="ACHUPG_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR0859817.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22592..24078
                     /locus_tag="ACHUPG_000019"
                     /pseudo
     CDS             22592..24078
                     /locus_tag="ACHUPG_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
     gene            24093..24827
                     /gene="ypdB"
                     /locus_tag="ACHUPG_000020"
     CDS             24093..24827
                     /gene="ypdB"
                     /locus_tag="ACHUPG_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR0859818.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24840..25697
                     /locus_tag="ACHUPG_000021"
                     /pseudo
     CDS             24840..25697
                     /locus_tag="ACHUPG_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25700..28202)
                     /gene="ptsP"
                     /locus_tag="ACHUPG_000022"
                     /pseudo
     CDS             complement(25700..28202)
                     /gene="ptsP"
                     /locus_tag="ACHUPG_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(28227..29271)
                     /gene="ypdE"
                     /locus_tag="ACHUPG_000023"
                     /pseudo
     CDS             complement(28227..29271)
                     /gene="ypdE"
                     /locus_tag="ACHUPG_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(29271..29687)
                     /locus_tag="ACHUPG_000024"
     CDS             complement(29271..29687)
                     /locus_tag="ACHUPG_000024"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFR0859819.1"
                     /translation="MTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQ
                     QIDDAARRVITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIY
                     LLGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(29684..29791)
                     /locus_tag="ACHUPG_000025"
     CDS             complement(29684..29791)
                     /locus_tag="ACHUPG_000025"
                     /inference="COORDINATES: protein motif:HMM:NF019360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0859820.1"
                     /translation="MARGAAARQSQRQDCCSGRVCHSRFWCAVSGLLLI"
     gene            complement(29752..30354)
                     /gene="ypdF"
                     /locus_tag="ACHUPG_000026"
                     /pseudo
     CDS             complement(29752..30354)
                     /gene="ypdF"
                     /locus_tag="ACHUPG_000026"
                     /inference="COORDINATES: protein motif:HMM:NF007310.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30369..31616)
                     /locus_tag="ACHUPG_000027"
     CDS             complement(30369..31616)
                     /locus_tag="ACHUPG_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFR0859821.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31638..31964)
                     /locus_tag="ACHUPG_000028"
     CDS             complement(31638..31964)
                     /locus_tag="ACHUPG_000028"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR0859822.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32183..33148)
                     /gene="glk"
                     /locus_tag="ACHUPG_000029"
     CDS             complement(32183..33148)
                     /gene="glk"
                     /locus_tag="ACHUPG_000029"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR0859823.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33352..34608
                     /locus_tag="ACHUPG_000030"
     CDS             33352..34608
                     /locus_tag="ACHUPG_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR0859824.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34723..35049
                     /gene="ypeC"
                     /locus_tag="ACHUPG_000031"
     CDS             34723..35049
                     /gene="ypeC"
                     /locus_tag="ACHUPG_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR0859825.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35190..36428)
                     /locus_tag="ACHUPG_000032"
     CDS             complement(35190..36428)
                     /locus_tag="ACHUPG_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR0859826.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36764..37966
                     /gene="nupC"
                     /locus_tag="ACHUPG_000033"
     CDS             36764..37966
                     /gene="nupC"
                     /locus_tag="ACHUPG_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR0859827.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38016..40128)
                     /locus_tag="ACHUPG_000034"
                     /pseudo
     CDS             complement(38016..40128)
                     /locus_tag="ACHUPG_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(40336..40411)
                     /locus_tag="ACHUPG_000035"
     tRNA            complement(40336..40411)
                     /locus_tag="ACHUPG_000035"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40376..40378),aa:Ala,seq:ggc)
     gene            complement(40451..40526)
                     /locus_tag="ACHUPG_000036"
     tRNA            complement(40451..40526)
                     /locus_tag="ACHUPG_000036"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40491..40493),aa:Ala,seq:ggc)
     gene            40747..41106
                     /locus_tag="ACHUPG_000037"
     CDS             40747..41106
                     /locus_tag="ACHUPG_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR0859828.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41108..41500
                     /locus_tag="ACHUPG_000038"
     CDS             41108..41500
                     /locus_tag="ACHUPG_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR0859829.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41552..42967)
                     /gene="gltX"
                     /locus_tag="ACHUPG_000039"
     CDS             complement(41552..42967)
                     /gene="gltX"
                     /locus_tag="ACHUPG_000039"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR0859830.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43226..43301
                     /locus_tag="ACHUPG_000040"
     tRNA            43226..43301
                     /locus_tag="ACHUPG_000040"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43259..43261,aa:Val,seq:tac)
     gene            43346..43421
                     /locus_tag="ACHUPG_000041"
     tRNA            43346..43421
                     /locus_tag="ACHUPG_000041"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43379..43381,aa:Val,seq:tac)
     gene            43426..43501
                     /locus_tag="ACHUPG_000042"
     tRNA            43426..43501
                     /locus_tag="ACHUPG_000042"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43459..43461,aa:Lys,seq:ttt)
     gene            43532..43657
                     /locus_tag="ACHUPG_000043"
     ncRNA           43532..43657
                     /ncRNA_class="other"
                     /locus_tag="ACHUPG_000043"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43777..44118
                     /locus_tag="ACHUPG_000044"
     CDS             43777..44118
                     /locus_tag="ACHUPG_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR0859831.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44109..45035)
                     /locus_tag="ACHUPG_000045"
     CDS             complement(44109..45035)
                     /locus_tag="ACHUPG_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0859832.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45125..46123
                     /locus_tag="ACHUPG_000046"
     CDS             45125..46123
                     /locus_tag="ACHUPG_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR0859833.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46120..46338)
                     /locus_tag="ACHUPG_000047"
     CDS             complement(46120..46338)
                     /locus_tag="ACHUPG_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR0859834.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46340..48355)
                     /gene="ligA"
                     /locus_tag="ACHUPG_000048"
     CDS             complement(46340..48355)
                     /gene="ligA"
                     /locus_tag="ACHUPG_000048"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR0859835.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48426..49412)
                     /gene="zipA"
                     /locus_tag="ACHUPG_000049"
     CDS             complement(48426..49412)
                     /gene="zipA"
                     /locus_tag="ACHUPG_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR0859836.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49642..50403
                     /gene="cysZ"
                     /locus_tag="ACHUPG_000050"
     CDS             49642..50403
                     /gene="cysZ"
                     /locus_tag="ACHUPG_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR0859837.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50588..51559
                     /gene="cysK"
                     /locus_tag="ACHUPG_000051"
     CDS             50588..51559
                     /gene="cysK"
                     /locus_tag="ACHUPG_000051"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR0859838.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            51943..52200
                     /gene="ptsH"
                     /locus_tag="ACHUPG_000052"
     CDS             51943..52200
                     /gene="ptsH"
                     /locus_tag="ACHUPG_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR0859839.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52245..53972
                     /gene="ptsI"
                     /locus_tag="ACHUPG_000053"
     CDS             52245..53972
                     /gene="ptsI"
                     /locus_tag="ACHUPG_000053"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR0859840.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            54013..54522
                     /gene="crr"
                     /locus_tag="ACHUPG_000054"
     CDS             54013..54522
                     /gene="crr"
                     /locus_tag="ACHUPG_000054"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR0859841.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54564..55415)
                     /gene="pdxK"
                     /locus_tag="ACHUPG_000055"
                     /gene_synonym="thiJ"
     CDS             complement(54564..55415)
                     /gene="pdxK"
                     /locus_tag="ACHUPG_000055"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR0859842.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55520..55894
                     /locus_tag="ACHUPG_000056"
     CDS             55520..55894
                     /locus_tag="ACHUPG_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR0859843.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            55927..56650
                     /locus_tag="ACHUPG_000057"
                     /pseudo
     CDS             55927..56650
                     /locus_tag="ACHUPG_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56854..57765)
                     /gene="cysM"
                     /locus_tag="ACHUPG_000058"
     CDS             complement(56854..57765)
                     /gene="cysM"
                     /locus_tag="ACHUPG_000058"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR0859844.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57899..58996)
                     /gene="cysA"
                     /locus_tag="ACHUPG_000059"
     CDS             complement(57899..58996)
                     /gene="cysA"
                     /locus_tag="ACHUPG_000059"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR0859845.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(58986..59861)
                     /gene="cysW"
                     /locus_tag="ACHUPG_000060"
     CDS             complement(58986..59861)
                     /gene="cysW"
                     /locus_tag="ACHUPG_000060"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR0859846.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59861..60694)
                     /gene="cysT"
                     /locus_tag="ACHUPG_000061"
     CDS             complement(59861..60694)
                     /gene="cysT"
                     /locus_tag="ACHUPG_000061"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR0859847.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60694..61710)
                     /gene="cysP"
                     /locus_tag="ACHUPG_000062"
     CDS             complement(60694..61710)
                     /gene="cysP"
                     /locus_tag="ACHUPG_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR0859848.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(62014..62805)
                     /gene="ucpA"
                     /locus_tag="ACHUPG_000063"
     CDS             complement(62014..62805)
                     /gene="ucpA"
                     /locus_tag="ACHUPG_000063"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR0859849.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(62934..63792)
                     /gene="murR"
                     /locus_tag="ACHUPG_000064"
                     /pseudo
     CDS             complement(62934..63792)
                     /gene="murR"
                     /locus_tag="ACHUPG_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
     gene            63956..64852
                     /gene="murQ"
                     /locus_tag="ACHUPG_000065"
     CDS             63956..64852
                     /gene="murQ"
                     /locus_tag="ACHUPG_000065"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR0859850.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64856..66280
                     /gene="murP"
                     /locus_tag="ACHUPG_000066"
     CDS             64856..66280
                     /gene="murP"
                     /locus_tag="ACHUPG_000066"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFR0859851.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            66285..66371
                     /locus_tag="ACHUPG_000067"
                     /pseudo
     CDS             66285..66371
                     /locus_tag="ACHUPG_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(66460..67359)
                     /gene="yfeX"
                     /locus_tag="ACHUPG_000068"
     CDS             complement(66460..67359)
                     /gene="yfeX"
                     /locus_tag="ACHUPG_000068"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR0859852.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(67455..68030)
                     /locus_tag="ACHUPG_000069"
     CDS             complement(67455..68030)
                     /locus_tag="ACHUPG_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR0859853.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68091..68540)
                     /locus_tag="ACHUPG_000070"
     CDS             complement(68091..68540)
                     /locus_tag="ACHUPG_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR0859854.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68527..68952)
                     /locus_tag="ACHUPG_000071"
     CDS             complement(68527..68952)
                     /locus_tag="ACHUPG_000071"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR0859855.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69166..70035
                     /gene="amiA"
                     /locus_tag="ACHUPG_000072"
     CDS             69166..70035
                     /gene="amiA"
                     /locus_tag="ACHUPG_000072"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR0859856.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            70039..70938
                     /gene="hemF"
                     /locus_tag="ACHUPG_000073"
     CDS             70039..70938
                     /gene="hemF"
                     /locus_tag="ACHUPG_000073"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR0859857.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(70944..71996)
                     /gene="eutR"
                     /locus_tag="ACHUPG_000074"
                     /pseudo
     CDS             complement(70944..71996)
                     /gene="eutR"
                     /locus_tag="ACHUPG_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(72042..72542)
                     /gene="eutK"
                     /locus_tag="ACHUPG_000075"
     CDS             complement(72042..72542)
                     /gene="eutK"
                     /locus_tag="ACHUPG_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR0859858.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72555..73214)
                     /gene="eutL"
                     /locus_tag="ACHUPG_000076"
     CDS             complement(72555..73214)
                     /gene="eutL"
                     /locus_tag="ACHUPG_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR0859859.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(73224..73292)
                     /locus_tag="ACHUPG_000077"
                     /pseudo
     CDS             complement(73224..73292)
                     /locus_tag="ACHUPG_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            74396..75187
                     /locus_tag="ACHUPG_000078"
     CDS             74396..75187
                     /locus_tag="ACHUPG_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0859860.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            75907..76302
                     /locus_tag="ACHUPG_000079"
     CDS             75907..76302
                     /locus_tag="ACHUPG_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR0859861.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            76320..76583
                     /locus_tag="ACHUPG_000080"
     CDS             76320..76583
                     /locus_tag="ACHUPG_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0859862.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            76606..77148
                     /locus_tag="ACHUPG_000081"
     CDS             76606..77148
                     /locus_tag="ACHUPG_000081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0859863.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            77287..77574
                     /locus_tag="ACHUPG_000082"
     CDS             77287..77574
                     /locus_tag="ACHUPG_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0859864.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77613..77819
                     /locus_tag="ACHUPG_000083"
     CDS             77613..77819
                     /locus_tag="ACHUPG_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0859865.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
BASE COUNT        20257 a        19958 c        18614 g        19273 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gctaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caagattaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattaattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatgattt ttatagcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg acgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag catttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaacaa gaaaatcacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttt aatccccttt
    22501 taaatttatg ttttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgctg gatatcgcca acaaaacgct accgctgttt
    23041 cgccatgtca atagcgagtc attacgcaag gtctgcgaaa ttatccgcga tgacattcac
    23101 gccgatgcgg tggcgattac taataccgat catgtgctgg cctatgttgg cgtgggtgaa
    23161 cataactatc agaatggcga tgacttcatt agcccgacta cccgtcaggc gatgaattac
    23221 ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca caccagagat tcattccatg
    23281 ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa cgctgaaaat ttactactgc
    23341 cacgcgcatc agatcacctc gtcattacag gaaatggcgg tcggtctgtc gcaaatcatc
    23401 tccacgcaac tggaggtttc acgcgccgag cagctacgtg aaatggcaaa taaggcagag
    23461 attcgcgccc tgcaaagcaa aattaatccc cattttctgt ttaacgctct gaacgctatt
    23521 tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt tgatctttaa tctgtcgcgt
    23581 tatctgcgct ataacattga attaaaagac gatgagcaaa tcgatatcaa aaaagagctg
    23641 tatcaaatta aagactatat tgccattgag caggcccgct ttggcgacaa gctgacggtt
    23701 atctatgata ttgatgaaga ggtgaattgc tgcattccca gcctgctgat ccagccgttg
    23761 gtggagaacg ccattgtcca cggtattcag ccttgcaaag gtaaaggcgt tgtcaccatc
    23821 agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc gagataccgg gcacggcatc
    23881 gttccaaagg tgattgagcg ggtcgaagcg aatgaaatgc cgggcaataa aattggcctg
    23941 ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg ggctgcatat ccgccgcctg
    24001 gagccgggga cggaaattgc gttttacatt cctaaccaac gcaccccagt cgcctcacag
    24061 gctacgttat tgctttgagc cggagtgata ttgtgaaagt catcattgtt gaagacgaat
    24121 tcctggcaca acaggaactg agctggctaa ttaaagagca cagccagatg gagattgtcg
    24181 gcacctttga cgacggtctg gacgtgttga agtttttgca gcataaccgc gtcgacgcca
    24241 tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt gctggcgcaa aacatcagcc
    24301 agttcgccca taaaccgttt attgtgttca tcaccgcgtg gaaagaacat gcggtagaag
    24361 cgtttgaact ggaggcgttt gactacattc tcaaaccgta tcaggagtca cgtattaccg
    24421 ggatgctgca aaaactggaa gcggcctggc aacaacagca gaccagcagt acgcctgccg
    24481 cgacggtaac gcgtgagaat gacaccatta atctggtgaa agatgagcga ataatcgtca
    24541 cgccaattaa cgatatctat tacgccgaag cgcacgagaa aatgaccttt gtctatacgc
    24601 ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt ttgcagcaaa ctgccgccgt
    24661 cgcatttttt ccgctgccat cgctcatttt gtgtcaatct gaacaaaata cgcgaaatcg
    24721 aaccgtggtt taataacacc tacattctgc gactgaaaga tctggatttt gaagtgccgg
    24781 tcagccgcag caaagtgaaa gaatttcgcc agttaatgca tctttaatcg ggaactttca
    24841 tgaaagcacc aggcttgccc gccgatcaac aatttttcgc cgatctgttc agcggcctag
    24901 tgcttaaccc gcaactactg gggcgcgtct ggtttgccag ccagcctgcc tcgttaccgg
    24961 tgggcagttt atgtattgat tttccccgtc tggatatcgt gctgcgcggc gaatacggca
    25021 acctgctgga agcaaagcag cagcgaatgg tggaaggaga aatgctgttt attccggcac
    25081 gcgcggctaa tttaccgatc aacaacaaac cggtgatgct gttaagcctg gtgttcgcac
    25141 cgacctggct tgggttatcg ttttacgata gccgcaccac gtcgttgttg catcctgctc
    25201 gccagaccca gcttcccagc ctgcaacgcg gtgaaggtga agcgatgctt accgccctca
    25261 cccatcttag ccgttcgccg ctggagcaga atatcattca gccactggtg ttaagtttgc
    25321 tgcatctttg ccgcaacgtg gtgaatatgc cgccgggtaa ttcacaaccg cgcggcgatt
    25381 ttctctatca cagcatttgt aactgggttt aggataatta tgcccagccg ctcacccgcg
    25441 agagcgtggc gcagtttttt aatatcacgc ccaatcatct gtcaaaactg tttgctcagc
    25501 atggaacgat gcgttttatc gagtatgtgc gttgggtgcg aatggcgaag gcgaggatga
    25561 ttttgcagaa atatcatctg tcaattcatg aagtggcaca gcgttgcggt tttccggata
    25621 gcgactattt ttgtcgcgtt ttccggcgtc agtttagtct gacgccggga gagtacagcg
    25681 cccgttttca gggctaacgt cagaaggtta attctgtttc cagcagcgtc aggatacttt
    25741 gtgcatcttg cgcggcaaac aacgactggc ggaagttttt attcaccagt ttacgcgcca
    25801 gctgcgagaa gactttcaca tgattcatcc cttcgttagc acccagcgtc agcatgatca
    25861 ccagttcgac ttcgcccatt tctgactgcc agtcaaccgg tttcgccagc cgggcaatgc
    25921 tgatactgga atgacggatc cactgagatt tggtgtgcgg gatcgctacg ccaaaaccaa
    25981 cgccggtggt aacaatctct tcccgctgcc agacatcttc ttccagctca aacggatgtt
    26041 cagtgcgccc gttaacgccg aggttgccgc acaggaactg gatcgcctgc tctttattgc
    26101 taaaatcctg atcaacaaag atattctcca gcgccagcag tgggcgaacg tcttcttccg
    26161 gcgtaaaggc ggtgagtaac gcttcaattt cctgcgcact gcggcattca catgcctgac
    26221 gcgccagttc ccgacacgcc tcgctatcca gttgacgaag ctggcttttc accgccggaa
    26281 tacgcgggct actcatactc agctcatcca ggcccagccc aagcagtagc ggcagataac
    26341 ggctttcacc gcccagttca ccgcaaatgc ctacccattt gccccgctga tgcgcagtgg
    26401 taactatttg ctgcaacatg cgcaggaacg atggcgtaat cgggttatat agcggcgata
    26461 cgcgcgggtt attacgatcg accgcataca gatactgggt catatcgttg gagccgatac
    26521 tgaagaaatc gacctcatcg cagaagtggt cgatgatgta gcacaccgac ggcacttcca
    26581 ccatgatccc aagcgtaatc gtctctgcat gacgcaggcc atcgcgctta agctcaacga
    26641 tcgctttttg gatctcgcct ttcacccata agatctgatc gaggctgtga accatcggga
    26701 tcatcaactg ggcgttgccg aaactggcgg cgcgcaaaat ggcccgcagt tgagtgcgga
    26761 acaggccagc aaattccggg taaatacgta ccgcgcgata gccgaggaac gggttctctt
    26821 cctggggaat attcagataa ggaatgcttt tatcgccgcc gatgtccatc gtgcggaaga
    26881 taatcggctt gtcgcccgcc gcccgccgcc agtagcacct gctggtaggc ttcaaattgc
    26941 tcctgctcgt ccggcgcgct gtcgcgatcc atatagagca tttcggtacg gaacagcccg
    27001 acaccttccg cgccgttggc aaacgcgcct ggcgcttcca gagcggtgcc gatattcgcc
    27061 gcaatatcaa tacgcttgtt atcacgggaa taggccagct gcgcggcagc ctgcgcctgt
    27121 tgtttttggc gtttatccgc cagcgtctgc gcgacctgat aataaccgct caccgcgtca
    27181 ttcgggttaa tcgccagcac gccgcactgg gcgtcaagca ctgcaggttg cccggcataa
    27241 cgggcaatcg catccagcgg caagccactc agtaccggga tcgccgaggc acgggccaga
    27301 atcagtgtat gcgaggtgcg cccggttttc tccagaatca tacccgcaag atttttcaaa
    27361 tcaaggctca aaaactggct tggggttaaa tcttcagcca ccagaatggt cggtttttcc
    27421 agcaccagat tgttgcgcgg cttcagttcc ggccaggtga tatgcagcaa ctgttcgctg
    27481 atatcgcgaa tgtcgctgac ccgctcgcgc agataatcgc tggcagaagc agatagtttg
    27541 gcgcaaacct gctccatatt gctgatgatc gccgccccca gtccctgatg ctgttctgtc
    27601 atcaggcgac ggatattgcc tgcaaattca tcatcctgaa tcagcgacaa atgagcgctg
    27661 aggatagttt tgctttcgcc gtcacgctca cgcaattgct gattcagttg ctcggcaagc
    27721 gttgccaggc tgtgctccag ccgggtggaa tcttgcgcac tggcggggat tgcccgataa
    27781 ctgtcgaggc tgtcgctttg taacagggtc agcgtaccca cgccgacgcc gcttgccagc
    27841 acattgccgt acagtaaatc cgggttaagg cggcttaatg aacgcggcag cggatgcgcc
    27901 gtcagttctg cctgcgtcgg ctgaacgctg tcgctgtcga taaagcgcac caggatgtac
    27961 tcttccagca cgcgccgcgc ctgctcttca tcgctgccgc taatgttcag gctgcaactg
    28021 tcattaaata aggtgccagt tccaatcaac gccagcgagc ttttagcatc agcctttgcg
    28081 ttctggcgat ggttgataaa ggtgatttcg ctttgccact ggctgcactg ttctttaagt
    28141 tcccacgccg gacgagcatg tagaccgtta ggcaaaggac agagaaattg aatcgttaac
    28201 ataaccgctc cgggttaatc aggagatcat ctgaaatccg tcagttgaac aaccgtctca
    28261 cgcgtaagac gttgaataag ggcagataaa agttgctcca tctgcaaaat gtcgcggcaa
    28321 tcggcaatcg gcaatcgatg ccgcgcaatg tccgtggcgg gtggcaggcc ccattaccaa
    28381 tgtgggtacg ccagtaccgg ttaagtgcac cgctccaccg tccgtgccgc cgttactgaa
    28441 catatccgcc tgcaacggaa cgccaatttc tgccgccacg gtttcaatcc acgcggtaag
    28501 ttttggcggc gcaatcagcg atttatcgct taacaccagc atcggcccgt taccaatctg
    28561 acgatggtta gccgcgccat aatcaaagtt tttcgcccag caggcggtat caagcacaat
    28621 ggcgacgtcc ggcgacaccg cgcgggtagc agtttgcccg ccgcgtaatc ccacctcttc
    28681 gctggaactt gccaccagcc acacttccgc aggcagttcg gcgtcgtgca gctcacgtag
    28741 taacgtcacc agcagatagc aactgaggcg atcatcaaag gctttgccca tcacccgctg
    28801 gtgagggagg acctgaaaag tggtatcgaa ggtgacgcga tcgcctggac gaattcccgc
    28861 ctgcatcact tcgtcatagg tgcgcgcgcc aatgtccacg cgcatggcgc taacgtcatt
    28921 cccctgccgg tcgccgtcaa gcaggcctgg aattttgcac tcttcacggg tggtgatgcg
    28981 caccggctgc agctggcggg cagccatgcg cacgttgcca accggcagca cgtcaatagc
    29041 gccttcgcgg gagatgctgc gcaccataaa tcccacttcg tccatatggg cgcagatcat
    29101 caccttcgga cctgtcgatt cattgaggcg gatcagcacc gatcccagac catcaaatcg
    29161 cacttctttt tgcaggcgag ccgcttcttc cagcaggatc tgccgcactt cctgttccga
    29221 ggaggcgatc gcatctgcct cgctcaacgc ttttaatagc gataaatcca ttatgcctct
    29281 cccgtgagca acactgtttt cggcatggcg tagagcactt ctgcgccttg cggggtaacc
    29341 agcacaacat cttcgatgcg cacgccccct tgccctagca aataaatccc cggctccacg
    29401 gtcagcaaca tgcctggctg tagcgtcgtg gtgtcccgcg gtgaaaaacg cggatcttca
    29461 tggacttcaa tgccgatagc gtgaccagtg ttatgaccga aatagtcgcc aaatccagcc
    29521 tcggtaatga cccggcgcgc ggcgtcgtca atctgctggc agcgcacgcc ggggcgaatt
    29581 gcagagattg ccgcgagctg tgcctgcagg acaatctgat agacgtcaaa cagcgggtga
    29641 gattcggcgc tcaccccttc gccattcacc agcaaggtgc gcgtcatatc agcagtagcc
    29701 ctgatacagc gcaccaaaat cgagagtgac aaactcgccc gctgcaacaa tcttgtcgct
    29761 ggctttgccg tgcggcagcg ccccgcgcca gccgctggcg acaatggtgt caaaagaggc
    29821 tttttctgcg ccctgctggc gcataaacca ctccagttca gcggctatct cgcgctcgct
    29881 catccccgcc tgaataaagc ggcgaatatg ctccgctccg cgatcggcaa tcccacaggc
    29941 gaggcggatt atctccacct cctctggcgt tttgatttgc cgcagcacat ccggcgtggc
    30001 gctaaccagt ttcgcattga gttcagactt ccagcggtgc gcggtttccc aactcacctg
    30061 ctggccttca aaaccgagcg tttgcaactg ctcatcggca atgatttgat tgacgatagt
    30121 ggtaagcgtg ttcgttgcgt caagcaaatg cagctggtag ccttgcgcac gggcttccac
    30181 ctcaacgaaa tagcgcgaat ctaccagaat gtgcgcactt tcacggctaa taaccacata
    30241 gcctgatccg gtggagatcc ccagatgcgg ctgtttgttc tgccgtgagg agagaagcac
    30301 cgcatccagt tgttgcacct taagccagtt gcgcagcgaa gcgagtaatg tcatctggat
    30361 tttccttctt acaggctatc gattaacaat ttgcctttgc ggaacatcat cagacgcagg
    30421 aacaccacca tcagcgcagt aatgattgcc cccagcgcga tccccgccat ataaacgccc
    30481 aagttagtga ccagcggcca tgcccagata gcggattccg ggaaccattg cactgcgccc
    30541 agccagacag ctgcggttga gccgacaatc gcgccgacca tatatgacgg aatagcggtg
    30601 atggggcttt ccagcgcaaa cggaatcgca ccctcactaa tccccataaa ggccaggaac
    30661 atcgcggtct taccctgcgg ataaagttga gcgttgaaca ggcgtttgcc ggttaaacga
    30721 cggtcaataa tggtcgccag acccagacca atcggcggaa taacgatagc gatagaacga
    30781 gcggtgaccg gtaatacgtg gtcagtggta aagctgaagg caacgaaacc tgcggctttg
    30841 ttgatcgggc caccaaggtc gattgctgtc gccgcggcga tccccattgc gtacatcagc
    30901 gcgcccttct cacctgcggc agtcagcaca gtacggatac cgccgttgat ccagccacca
    30961 aagggagtga tgacgtagta catcgccagc atgacaaaaa tggcggaaag aatcggtagc
    31021 aaaaatgtgg ttttgaacgc taacaggaaa tccggtaact ggattttctg gttcatccat
    31081 ttcacaaggt aaccggcaac gatagaaata atcagcgcac caatgaaggt ggacggcacc
    31141 ggcgaagagg tcgcccattg catagtgctg ggatcgaagt tcagcagttg cgtcggctgg
    31201 gtagacatca acccgccgat aaaaccagcc gggaacgcca gtttgccacc gatagagttc
    31261 gccacaaatg cagcaaacat cggaatggca aaaccaaaca acacgccgcc gaaggactgc
    31321 gacagccagg caaatttaag taaggagagg tcgaaaccgg agaatttccc gctattaaga
    31381 gcatccatga tgccgatctc agcaggaatt ttaagccagc tataagcaat cagctggctg
    31441 aaagcgagga tcacaccgcc cataattaag gtcggcacca tgcgtgaaat ccctgacatc
    31501 acatgctgcg ggagttcacc ccagaagctg cttttagagg cctgcggatt cttaactgcc
    31561 gctgccgcac cggatgcgcc aggcacaacg gttgcactgc gttttttaat ggccataaca
    31621 attatccctg tcgaatatta ctgttgttca gaggcaatca tctcttcgat ttctttgatg
    31681 atgcccgcag cgtttttaat tgcgtcctgc aaagtgattt cataaacgtc gcgtgattcg
    31741 aaacgttcgt tatcttccgg ggtaactgcc acggaatgga tgatgatggt cgcttcggcg
    31801 atatcctgcg ccgtcaggcg attctggatg ccgtccgcgc cctgggtttc aattttcact
    31861 tcataaccgg cttctaccgc cgcttcttcc agcacctgag cggccataaa agtgtgagcc
    31921 aggcccattg ggcaggcaca taaggcaatc agtttcttac tcatcgttaa ttcctcgttc
    31981 agtgatagtt gactagtgaa attatgaacg tgcaaaacaa atcgccgtta ccagacaaat
    32041 aatgcattta ctggagaatt aatcccgctg tacggaagtg tgacgcaggt cggccttgtg
    32101 aggggacaca aagaaaaatt gaattaactg tatgatttaa aagattatcg ggagagttac
    32161 ctcccgatat aacaggaagg atttacagaa tgtgacctaa ggtctggcgt aaatgtgcac
    32221 cggaaccgag aagacccgga tagtcatgga cgatgagata caccggaata tcatggacat
    32281 attctttaaa gcgcccttta tcttcaaatg cggcacggaa accggaggct ttgaagaact
    32341 caaggaagcg cggcacgata ccgcccgcaa taaacacgcc gccaaatgtc ccgagattga
    32401 gcgccagatt gccgccaaaa cggcccataa tgacgcaaaa cagcgacaat gcgcggcggc
    32461 aatcggtgca gctgtcagcc agcgcgcgtt cggtaatatc ttttggcttg agattttctg
    32521 gcaggcggtt gtcagctttc acaattgcgc gatacaaatt caccagccca gggccagaaa
    32581 gcacgcgctc cgccgaaaca tgaccaattt ccgcacgcaa tatttcgagg ataatggcct
    32641 cttcttcact attcggcgca aaatcaacgt gaccgccttc gcccggcaag cttacccaac
    32701 gcttatcgac atggaccaga tgcgcaaccc caagccccgt tccggcaccg taaaccgcaa
    32761 taggcttacc ttcgaccggt tctgcgccac caaactgaat cagatgctct tttttcagca
    32821 tcgggatcgc catcgataca gcggtaaaat cgttaataat ttccagatgg ctaaaaccga
    32881 gattcttttt catttcggca attgagaacg cccaggtatg gttggtcatt gccacccagt
    32941 caccggtaat tgggcaagcg atggcaatac agccgtcttt cacctcgacc ttatgttctt
    33001 caagataaac gcgaatgacc gcttcgaggc tggggtaatc aagccctgaa taggtcttag
    33061 cctgcgagat ttcaccactg gcaatatcac acagagcaag acgtgcgttg gtgccgccca
    33121 catcaccgac taatgcatac tttgtcattc ttcaactgct ccgctaaagt caaaataatt
    33181 ctttctcaca ctgtaaatac ctgggggcat aacaacaacg ccggaaaggc aggctccctg
    33241 taaatatcga tctgggtcac acaattactt tatcgtttca gcaccaattg cagcgatgcc
    33301 tttttgcaag ctgggcaaac taagtatctg accccgcata aggaatagaa catgctccat
    33361 ccgcgagcca gaaccatgtt gttattatcg ctccccgccg tggcaattgg gattgcgtcc
    33421 agtcttattc tgattgtggt gatgaaaatc gcctcggtat tacagaattt gctctggcaa
    33481 cgactgccgg gaactctggg gatagcccag gattcacccc tctggatcat cggtgtatta
    33541 acgctaacgg gtattgcggt ggggttggtt atccgtttca gccagggtca tgccggacca
    33601 gaccccgcct gtgaaccgct gatcggcgca ccggttccgc cctctgcgct acctagactt
    33661 atcgtagcat taattctcgg tcttgctggc ggcgtcagcc tggggccgga acatccgatc
    33721 atgaccgtca atatcgccct tgccgtcgcc attggcgctc gtctgttacc gcgcgtcaac
    33781 cgaatggagt ggactatttt agcctctgcc ggaaccatcg gcgcgctgtt tggtacacct
    33841 gttgcggcgg cgttgatatt ttcgcaaacc ttaaatggca gtagtgaagt tccgctatgg
    33901 gatcgtcttt ttgcgccgtt aatggcggca gcagctggtg cacttactac cggattattt
    33961 ttccatcccc atttttcact gcccattgcg cattacggac agatggaaat gaccgatatt
    34021 ctcagcggtg caattgtcgc ggcgattgcc atcgcagcag ggatggtcgc cgtatggtgc
    34081 ttaccacggt tgcacgcgat gatgaatcaa atgaaaaatc cggtgctcgt gctgggtatt
    34141 ggcggattta ttctcggaat tctgggggtt attggtggac cagtttcgct gtttaaaggg
    34201 ctggatgaga tgcagcagat ggtggcaaat caggctttca gcaccagcga ttactttttg
    34261 ctggcggtaa ttaaacttgc cgccctggtc gttgctgccg ccagtggctt tcgcggtggg
    34321 cgaatcttcc cggcagtgtt tgtcggcgtg gcattagggt tgatgctgca tgagcacgtt
    34381 cccgccgttc cagcggcaat aaccgtttcc tgcgctattc tcggcatcgt gctggtggta
    34441 acacgcgatg gctggttaag tctttttatg gcggcagtcg ttgtacccaa taccacattg
    34501 ctaccgctgc tctgtatcgt catgcttccg gcatggctgt tattagcagg taagccgatg
    34561 atgatggtca atcgtccgaa gcaacagcca ccccacgata atgtttagca aaaaatgctc
    34621 tcctttatga tttaagagtt atggcgatga tacggggctt tacctccccg taatattgcg
    34681 ttaacaggcc gctgacggct tcgatcgtga aggagaataa caatgttcag gtcactgttt
    34741 ctggcggccg ccctgatggc atttaccccg cttgcagcaa atgcaggtga aatcacccta
    34801 ctgccatcga tcaaattaca aattggcgat cgcgatcatt acggtaatta ctgggacggt
    34861 ggtcactggc gagatcgtga ttactggcat cgcaattatg aatggcgcaa aaaccgctgg
    34921 tggcgtcatg ataatggcta tcatcgtggc tgggataagc gtaaagcata tgagcgtggc
    34981 tatcgtgaag gctggcgcga tcgtgacgat catcgcggaa aaggccgcgg acatgggcac
    35041 cgccattaaa cgcgtcctcc aatggagcac aatgcctgat gcgacgctag cgcgtcttat
    35101 caggcctaca aaccggctct ttaacgcgta gggcggataa ggcgtttacg ccgcatccgc
    35161 caatagtgcc agatgcgacg ctcattcaac tacaatccca gcgccgtccc caccaacaac
    35221 cagatattca gcgccacgac cagcactaca atcacccagc ctgtctgttt tacgcgtttg
    35281 ctgttcacca gatcgcccat caacttgctg tcactggtga aaatcagcag tggaaccagc
    35341 gccagggcga taccaaaact taacagcacc tgactcataa ccagaatccg tgtcggatct
    35401 aatcccatca gaatgacaat aaatgacggc aacatggtga ctgtacgacg cacccacagc
    35461 gggatatgga agcgaatgaa tccctgcatc accacctgcc ccgccagtgt ccccaccacc
    35521 gttgaggaca gtccggcagc aaccagactt aacccaaaga ccgttgccgc agcatggctt
    35581 aacagcggtt gcagcgtcag ataagcctca tcaagatcgg caacaccagt atgaccagaa
    35641 aagtggaacg ccgccgcagc tgtagccatc atcaccagat tgacaaaacc ggcaatcgtc
    35701 atggcgatag ccacatccca tttggtggcg gaataacgtt gttgacgcga accgccatgt
    35761 aaatgctgag tgagcgaaga gtgcaaataa atcacatgcg gcataatcgt cgcccctaac
    35821 acgcctgctg ccaggaagac cgcctccgaa gtaggtaaac tcgggatcac cattccttta
    35881 cccagctgcg ccaggttagg ctgggagaaa atcaactcga caatgtaagc cgcggcaaca
    35941 aacaacagta acccgccaat cactttctcc agcggttttt gcccgcgacg ttgcagcatt
    36001 aaaatcagga aagtcgcgat ccccgtcagc accgcgccct gcaacaacga aacaccaaga
    36061 atgagtttaa aaccgatcgc cgcaccaata aattccgcca ggtcggttgc catcgcaata
    36121 atttctgcct gaacccaata gaaccacact acgggacgcg gatagtgatc acgaatctgc
    36181 tccgccagat ttttaccggt ggcaatccct agtttggcag agaggatctg aatcagcatc
    36241 gccatcaggt tggcccaaac gacaacccac agtagctgat agccgaagct agcacccgcc
    36301 tgaatattgg tcgcaaagtt accgggatcg atataaccaa tcgccgcaat gaacgcaggt
    36361 cccattaatg cgagcctcat cttacgcgcc gcccgtccgc tgctactctc aacgcgatag
    36421 ttcgtcatct tgtgcctcta aaacatagcc tttgctatgt ttcatgctat gctaaacgag
    36481 aatgattatc aaattcattt aaatggattg tggtgatttc tctgatagac caagattatg
    36541 attacgaaaa gattgatgac ttgaatgtta tggttatgtt taatgttagc acatttacat
    36601 aactttcagc ttccatacac aacatagcag aaatgtatga cagatcacta tttttgaagc
    36661 ctgtcacagg acgtcattat agtgtgtgtc agatctcgtt ttccttaacc atgttacata
    36721 gaatgtgcac ggaaatttaa cctgcctcat atttggagca aatatggacc gcgtccttca
    36781 ttttgtactg gcacttgccg ttgttgtgat tctcgcactg ctggtaagca gcgaccgcaa
    36841 aaaaattcgt atccgttatg ttattcaact gcttgttatc gaagtgttac tggcgtggtt
    36901 cttcctgaac tccgacgttg gtttaggctt cgtgaaaggc ttctccgaaa tgttcgaaaa
    36961 actgctcgga tttgccaacg aagggactaa cttcgtcttt ggtagcatga atgatcaagg
    37021 cctggcattc ttcttcctga aagtgctgtg cccaatcgtc tttatctctg cactgatcgg
    37081 tattctccag cacattcgcg tgttgccggt gattatccgc gcaattggtt tcctgctctc
    37141 caaagtcaac ggtatgggca aactggaatc ctttaacgcc gtcagctccc tgattctggg
    37201 tcagtcagaa aactttattg cctataaaga tatcctcggc aaaatctccc gtaatcgtat
    37261 gtacaccatg gctgccacgg caatgtccac cgtgtcgatg tccatcgttg gtgcatacat
    37321 gaccatgctg gaaccgaaat acgtcgttgc tgcgctggta ctgaacatgt tcagcacctt
    37381 tatcgtgctg tcgctgatca atccttaccg tgttgatgcc agtgaagaaa acatccagat
    37441 gtccaacctg cacgaaggtc agagcttctt cgaaatgctg ggtgaataca ttctggcagg
    37501 tttcaaagtt gccattatcg ttgccgcgat gctgatcggc tttatcgccc tgatcgccgc
    37561 gctgaacgca ctgtttgcca ccgttactgg ctggtttggc tacagcatct ccttccaggg
    37621 catcctgggc tacatcttct atccgattgc atgggtgatg ggtgttcctt ccagtgaagc
    37681 actgcaagtg ggcagtatca tggcgaccaa actggtttcc aacgagttcg ttgcgatgat
    37741 ggatctgcag aaaattgctt ccacgctctc tccgcgtgct gaaggcatca tctctgtgtt
    37801 cctagtttcc ttcgctaact tctcttcaat cgggattatc gcaggtgcag ttaaaggcct
    37861 gaatgaagag caaggtaacg tggtttctcg cttcggtctg aaactggttt acggctctac
    37921 cctggtgagt gtgctgtctg cgtcaatcgc agcactggtg ctgtaaaacc atacataaaa
    37981 aagccgggga tcattccccg gcttttttac gcctgttaat cagctaatgg ctgcgggcga
    38041 ccaatcaaat acccttgcag atattgcacc ccgagcttat gcaatagcgc ctgctgctgt
    38101 tgcgtctcga caaactccgc gaccacactc aatgacttcg ctttcgccag atcggtaatt
    38161 gatctcacaa tcatcgcatc cagcgtattc gtgacaatat ctttcacaaa gacgccatca
    38221 attttgatga tatcagcctg caaacgcttt aaccgttcgt agttggcata tccggtgcca
    38281 aagtcatcaa tcgcaatccg gaaaccaaac ttatgcagct gctcgatgtt gtacatgctg
    38341 ctttctgcgt tagaaaacgc ctgctcctcg gtgatctcaa gaatgaccgc ctgcggggag
    38401 atgtgataac gcttaaacag acgaataatc cgcccggcaa tattcttttg cagcagcgtg
    38461 agcggcatta aattgactga aaagcgcggt ccttttttgt cgcaagggtg tgttgccagc
    38521 cacttcaaca aggattccag cacttgcaaa tcaaaacgcg cgctaaggtt aaactgagca
    38581 ataaggggca gaaacttatc cggggtcata atgccgccgt catatttcag tcgcgcgagg
    38641 atctcatcat aaccttcacc ctctttgttg cgaattggct gggcgtagag cagcaaatca
    38701 ccctgatcta acgccgtgcg aatggtattc agcaatagca cctgtttagt ggtctgcccg
    38761 gaaaccatct cctctctgct atccagcgcc agcacatgat gatgtgcgca ggattgctcc
    38821 gccagccagc ttaactgccc caacaagggt tgcagggttt cctgatttcc atcaaaacgc
    38881 ccccaggcag caccatagcc catatccagc ccggtattgt tccagtgaat ttgccgacta
    38941 ttcaggatgt taaccatatg ctggagtcgc ccttccgttt ccggcccgct cagcactaac
    39001 agcagttcac tacccggcaa ttgatacaac ttttcgtttt cctgcatcaa cggcagcagc
    39061 gtacggcaaa ttgagcggat acagtgaacg cgcatcatta agccgtaatg acgactcata
    39121 aactcaagat tatcaatgcg caggcagcaa aaactcttgc ccgcctcttg ctccggcgct
    39181 tgttccaacg cacgaaagtt gggtaggagc gttaacggat ctgttagcgc ctgcaaatgc
    39241 cagcggcgat taagccattc actgcgatga taaatgcgca ccatatagag caggcaaacg
    39301 ctaaaggaaa tcagcaccgc gagaataaat gccagcgaat attcggtttc caccccttgc
    39361 aaaaagttct gattgtaatt cagaaggcaa agcgttgaaa cagcccaggt gagatttaaa
    39421 aacggatagc gaagattacc gaccccgagg gtaaagatga tgaagaaaac gggtaccagg
    39481 taaccggcaa taaagtcgtt ttcataaggt gtgcacaaca gaagtagcag cacgcttaac
    39541 gctgccagcc agcttaaggt aaatgcgagt gagatagtag aaaagcatgt tgtaaatcag
    39601 cactgcggtg aaaaggctta gtaaatcaac gaccgtgaaa atggcatccg catcgccgaa
    39661 aaaggtggat atctttagcg ggaaatcaaa gaagctcccc acaagataca tgctgcattt
    39721 gatgccgatc ggcgtcacca aaccaagcca gaacagacgt tgccagacat atcggctggt
    39781 caatccataa cgccaacgcg tccccagctg ccagcgtaat attgcgcagg cacacaacac
    39841 gacaaacgtc tggctgccga gcatgaccgc agtctgtaaa aaagagagat gaaagttcca
    39901 cagattggtg caaaacatcc cgcataaaac gggaacgaca ccgcgccagc caaagataaa
    39961 caagattgac agcattacgc acagtggcat ccacgccaga aagatataac tggaatggat
    40021 aatggcaagt ggcgaaataa aacgggatag ctgaataagt accacggtga gcgtaaacgc
    40081 tagtgtgaat atcttgatat tttttatcag gttatgctcc acaaacatga gtacactact
    40141 ctcactgatc ggggttttca ggcacacaaa taataagttt attcgcatcg cgaagcaaac
    40201 aggggccagc ctgttgccga tttttcatcc aatgatgctt tcaccaggcg gcgctaaccc
    40261 ggacgtagac tgtattacaa aagcggcaaa aagcagagac aaaaaacccc cgctttgcag
    40321 cgagggttgg aaatttggtg gagctaagcg ggatcgaacc gctgacctct tgcatgccat
    40381 gcaagcgctc tcccagctga gctatagccc cacgatgcgt ttacgtacca aatttgctgg
    40441 gtgcaaaatt tggtggagct aagcgggatc gaaccgctga cctcttgcat gccatgcaag
    40501 cgctctccca gctgagctat agccccgtca cgtaaagctt gtcgagttga cgggcggcat
    40561 catatgaatt ccgcccgaat gtgtcaacgg caaattgcaa cgtgtagttt caatcgctga
    40621 aaaatcaggc aaatgaacaa ttttggaaaa ccgctcgcat tcagtagtta ttcatgtcac
    40681 ggtttcctgt aaagtggtgt tataaaatga actactaata gacccacata cattcaggga
    40741 attgttatgt tcaaggagag gatgacacca gatgaacttg ccagactgac cggctatagc
    40801 cgccagacca ttaataaatg ggtacgcaag gaaggctgga caacgtcacc aaaacctggc
    40861 gtccagggtg gcaaagcccg actggttcac gtcaatgaac aggttcgtga atatattcgc
    40921 aatgctgaac gtccagaagg tcagggagaa gcgcctgccc tttctggtga tgcgccgctt
    40981 gaagtattgc tggtgacact ggcgaaagag atgacgccag ttgaacaaaa acagtttaca
    41041 tccttgcttc tgcgggaagg gattatcgga ttgttacaac gcttagggat tcgcgatagc
    41101 aaataatatg aaaagattac gcaataaaat gaccaccgaa gaactggctg aatgcctcgg
    41161 tgtggctaaa caaactgtta accgttggac cagagaaaaa ggctggaaaa cggaaaaatt
    41221 tcccggcgtg aaaggcggtc gtgccagact tattctggtc gatacgcaag tttgcgagtt
    41281 tattcagaac acgccagcct tccataacac accaatgttg atggaagctg aagagagaat
    41341 tgctgaatac gctcctggag cacgcgcccc ggcgtatcga caaatcatca acgcgataga
    41401 taatatgaca gacattgagc aggaaaaagt cgcgcaattt ttgtcacgtg aaggaattcg
    41461 caatttcctc gcccgtctcg atattgacga atcagcataa ataaaaaacg gcaggatatt
    41521 atctcctgcc gtttatcttt ttacacgcta attactgctg attttcgcgt tcagcaataa
    41581 aatccagcgc tttgttgata cgctcgatac tgcgggtctt accaattgcg tgaacggtaa
    41641 catccagtgc tggagactgc cccgcaccgg ttacggcgac acgcagcggc ataccaactt
    41701 tacccatacc cacttccagc tcatccgccg tcgcctgaat agcgtgatga acgttttcag
    41761 cggtccagtc agtaatcgcg gccagtttgt cacgaaccac ttccagcggc tgacgcgcta
    41821 ccggacgcag atgttttttc gccgcatcgg catcgaactc agcaaaatct tcgtagaaat
    41881 aacggcagct ctgtgccatc tctttcagcg tcttgcagcg ttcgcccagc agtttcacca
    41941 gatcagccag ctgcgggccg ttacgggtat cgatattttc ctgctcaatg tgccactgta
    42001 agtgagtagc aacatactcc ggcggcagcg cgttaatgta gtgatggttc agccacagca
    42061 gcttgtcggt gttgaacgca ctggcagatt tgctgacggc attcaaagtg aagtatttga
    42121 tcatctcttc acgagtgaag atttcctgat cgccgtggga ccagcccaga cgcaccagat
    42181 agttcagcag tgcttctggc aaataaccgt catcacgata ctgcattacg ctgactgccc
    42241 cgtgacgttt ggacagtttt ttaccgtcat cgccattgat catagaaacg tgcgcgtaaa
    42301 ccggcaccgg cgctttcagg gccttaagaa tgttgatctg gcgcggcgtg ttgttgatat
    42361 ggtcttcgcc acggataacg tgggtgattt ccatatccca gtcatcgaca accacacaga
    42421 agttataggt tggggaacca tcggtacggc ggataataag atcgtccagt tcctggttgc
    42481 tgaactcgat cggaccacgg atctgatcgt caaaaacaac agaaccttcc tgcgggttag
    42541 caaaacgtac aacacacggt tcatcatcag catgatgctc atggctgtgg cggcagcgac
    42601 cgtcataacg cggcttctca cctttcgcca tttgctcttc gcgcagcgct tccaggcgct
    42661 ctttagagca atagcattta taagcagtgc cctcttccag catctgatcg atcaccgcgt
    42721 tgtagcgatc aaaacgtttg gtctggtagt acggaccttc atcccactcc aggctcagcc
    42781 agttcatgcc atccataatg gcttcgatag cttccggcgt ggaacgctca agatcggtgt
    42841 cttcaatacg cagcacgaac tcaccgccgt ggttacgtgc aaaaagccag gagtaaagag
    42901 cagtacgcgc gccgccaacg tgcagatagc ctgttgggct tggcgcgaag cgagttttga
    42961 ttttcatgaa atggccttac gtttagaaag atgccgacaa ccggcaaatc ctggaaaaat
    43021 tgagtgggcg atattctatc actcccgcct gattcctcaa tgtagttcgg gtttctacct
    43081 tacgcattat tgttttcgtt gacaaattgc gcaacacggt ctgttttgcg atcatcttgc
    43141 ttaaatttac gacgaacgaa caatttcttt agaaaatgcg ttgactcatt ttgaactctc
    43201 cctataatgc gactccacac agcgggggtg attagctcag ctgggagagc acctccctta
    43261 caaggagggg gtcggcggtt cgatcccgtc atcacccacc aactacttta tgtagtctcc
    43321 gccgtgtagc aagaaattga gaagtgggtg attagctcag ctgggagagc acctccctta
    43381 caaggagggg gtcggcggtt cgatcccgtc atcacccacc acttcgggtc gttagctcag
    43441 ttggtagagc agttgacttt taatcaattg gtcgcaggtt cgaatcctgc acgacccacc
    43501 aatgtaaaaa agcgccctaa aggcgctttt ttgctatctg cgatactcaa agattcgaac
    43561 ctgcagcagg tttgagttga gcgcagcgaa acaacggagc cgctcgcggc gacggcccga
    43621 agggcgagcg aagcgagtca tcctgcacga cccaccaatg caaaaaagcg ccctaaaggc
    43681 gcttttttgc tatctgcgat ttgtaagatt gatttttctt ctcaagttcc ttgttcttct
    43741 accgatatta cctcgtacag tgacgaggac tgaacaatgg ccactatctc ttcaacttca
    43801 attccttcca tacaaacaca gagtagtaac agggcatcac aaggtagcga tgttgcttca
    43861 caaatcgcgc ggataagtca gcaaatcatc aaactcactc aacaaattaa agagattgta
    43921 gacactagcg gcagtgctga agacaagcaa aaacaggccg aattgattca acagcaaatc
    43981 accctactgg aaacacaact ggcgcagttg cagaaacagc aggcagaaaa ggcgcaggag
    44041 aaagagcagc gtttgtctct gaatgtatcc cttctaaatc ctgttgaaaa aacgactcat
    44101 atcgatatct atatctgata caacggatcc ccttcccgac cagccattag cgcattgccc
    44161 tggtctcgta ccacgtccca taacgcttca gcggcggtgg aaagtgaacg gttcttcctg
    44221 cgcaccagca tcaactggcg ttcaaccacc ggcgtaatgc gcttaaccac cagtgggcta
    44281 ccttcaggca atggtagcgc cagtgcagga agaatactga tgccgatgcc tgcggctacc
    44341 atcgggaaca gcgtcgccgg atgtccaatc tcctgcacaa tattcgcctg aataccattg
    44401 cgcgccagcg ctgcgtcaat cagcggtcgg ctgcctgacg cgtaatcctg caacaccaat
    44461 ttcgctccct gtaatgcctg ccagggtaca taatcttcca cggccaaagc gctatcgcga
    44521 tggcaaagca gaaagaaagg ctcggaaaga atcgcttcac attgcagatc gcccactggg
    44581 cctggatcga tgacgatgcc aaagtcgaca tctccctggc gaatactttc catcacccac
    44641 tgctgcggac gatcatgcag gacaaactga atatctggat agcggcgatg gctttcagca
    44701 atgcattgcg gaataagatg cgcggaaatg gtctggctgg cagcgacccg aacttttccg
    44761 ctcagttgtt gccccatacg cccggtatcg cgcaacgtgc tgttaagttc atccagcagt
    44821 cgctcaagac gcaaagccaa ctgctgccct gcatctgtaa gcaccacttc acgcgtggtt
    44881 ctgtccagca ggcgaacacc agtatgattt tccagctcct tcacactgtg acttactgcc
    44941 gactggctca agccgatacg ctctcctgca cgactaaaac ttttctcctg cgctactgtg
    45001 acgaaaacct ttaattgttt taaagaataa ttcatctgtt ttcttcatga atggatgcaa
    45061 taaatcaatt ttatttctca aactggaaga agcacaatag aaccatcgat catctggagt
    45121 ctttatgaaa ctttttcgta tcctcgatcc tttcacctta accctgatca cggtggtgtt
    45181 gctggcctct ttctttccgg ccagaggcga tttcgtcccc ttctttgaaa atctgaccac
    45241 cgcagctatt gccctgctgt tctttatgca cggcgcgaag ttgtcgcgtg aggcgattat
    45301 tgctggcggt ggtcactggc gactgcattt gtgggtaatg tgcagcacct tcgtgctgtt
    45361 tccgattctg ggtgtactgt ttgcctggtg gaaaccggta aatgtcgacc cgatgctcta
    45421 ctccggtttt ctctacttgt gcattctccc ggctaccgtg cagtctgcaa tcgccttcac
    45481 gtcaatggcg ggcggtaacg tcgcggcggc ggtttgttct gcgtcagcat ccagcctgct
    45541 ggggattttc ctttcaccat tgctggttgg tctggtgatg aatgttcacg gtgcaagggg
    45601 cagccttgag caggtcggta aaattatgct gcaactgctg ctgccgtttg tgttggggca
    45661 tctttcccgg ccgtggattg gtgactgggt gtcgcgcaat aaaaaatgga ttgcgaaaac
    45721 tgaccagacg tccattctgt tggtggttta tacagcgttc agcgaagccg tcgttaatgg
    45781 tatctggcat aaagttggct ggggatcatt gctgtttatc gtggtggtca gctgcgttct
    45841 tctggctatc gtgattgtag ttaacgtctt tatggcacgc cgactgagct tcaataaggc
    45901 agatgaaatt actatcgtct tttgtggttc gaaaaagagt ctggcaaatg gcatcccgat
    45961 ggcaaacatt ctgttcccca catcggtgat cggtatgatg gtgctgcccc tgatgatttt
    46021 ccatcagatc caattgatgg tctgtgcggt gctggcgcgt cgatacaaac gccagaccga
    46081 acagttacag gcgcagcagg aaagcagcgc cgataaagct taaagcggac gcttcagggg
    46141 ctggaccagt tgcgtcagcc cctcggtttt aatcagcagc gtgatttgca ttagctcacc
    46201 gagcttccct gccgggaact catctttgcg ggcaaaccac agcagatact cctccggcag
    46261 gtcaattaag cgacgccctt tgtatttgcc aaacggcatt atcgtattgg ctatttcaat
    46321 cagctgctct ttttccatct cagctaccca gcaaacgcag catttccgct tcgtcgatga
    46381 cttcaatgcc cagttcctgc gccttcgcca gtttagatcc tgcagcttca cccgctatca
    46441 ccagatcggt tttcttcgac acgctgcccg cgactttcgc gcccagttcg accagtcgag
    46501 ctttagcgtc atcacgcgac atctggctta agctgcccgt aagcaccacg gttttaccag
    46561 caaacgggct gtcaatctct tccgcgttga taacgatcgg cgcaggccag tgaacacctt
    46621 ccgccaacag ctcgctgatg acattgcggt tgctttcttc ggcaaagaag ttgtgaacgt
    46681 gggatgcaac gacaatgcca acatcaggca ccttttgcag ctcttcaatc gaagcggctt
    46741 ccagcgcttc cagcgtgccg aaatatgccg ccagacctgc tgcggtggcc tcgccgactt
    46801 cacggatgcc aagtgcatag aggaagcgag caaaggtggt ttctttcgct ttttccagcg
    46861 cgttaaccac gttttgtgcc aattttggcc ccatacgctc cagtccggtc agtttgcctg
    46921 cggtgagttt gaacagatct gccggagtat ggacatattc tttttcaacc agctgatcga
    46981 tgattttgtc gcccattccg tcaacatcca tcgcacggcg ggaaacaaag tgtttcagcg
    47041 actctttacg ctgcgcaccg caaatcaggc caccggtaca gcgggcaacc gcttcacctt
    47101 ccacacgctc aacgtcagaa ccacataccg gacaatgcgt cgggaataca acctcacggg
    47161 tatcttccgg gcgttcagaa agcacgacgt taaccacctg cgggatcacg tcgccagcgc
    47221 ggcgaatcac cactttatcg ccaatgcgta aaccaagacg ttcgatttca tccgcattgt
    47281 gtaaggttgc gttactcacc agcacgccag caacatggac aggttccaga cgcgcaacag
    47341 gcgtaatcgc gccagtacgc ccaacctgaa actcgacgtc acgcacaaag gtcatctgct
    47401 cctgcgccgg gaatttaaac gcaaccgccc agcgcggggc acgcgcgaca aagccaagct
    47461 gctcctgctg ttccagtgag ttgaccttaa tcaccacgcc gtcgatatca aagcccagcg
    47521 tcgggcggtc ttcttccact ttgtgataga acgccagcac ttcttccgcc gattcacaaa
    47581 gcgttacccg atcgctgacc ggcacccccc actttttaaa ttgcagtaaa cggccaagat
    47641 gagtatccgg cagctcgcca ccttccagaa caccaacgcc atagcagaaa aaagtgagcg
    47701 gtcgcttcgc tgtaatacgc ggatcaagct gacgcagtga accagctgcc gcattacgtg
    47761 ggttagcaaa aactttcccg cccgtgcgtc gcgcatcttc gttaatcttt tcgaaccccg
    47821 cctgcggcag gaacacttca ccacgcactt ccagacgcgc cgggatattc tctccgtgca
    47881 gcttcagcgg aatggcgcga atagtacgca cattagacgt gatatcttcc ccggtggtgc
    47941 catcgccacg ggtcgcggca ctgactaaaa cgccattttc atacagaata ctgacggcaa
    48001 ggccatccag cttcagctca cagcaccagg tgactttctc gttgtttttc agacggtcct
    48061 gcacacgttt gttgaaagca agaaagcttt cttcatcaaa aacgttatcc agtgacagca
    48121 ttggtacttc atggcgtatc tggctgaaag ctgccagcgg cgcagcacct acacgttggg
    48181 taggcgaatc aggcgtaatc agttctggat gtttggtttc cagctcgcgc agttcgcgca
    48241 tcagcctgtc gtattcagcg tcgggaattt ccggcgcatc catcacatga taaagatatt
    48301 catgatggca aagcgtcgtt cgcagttctg tcagttgttg ttcgattgat tccatatcgc
    48361 accatcaatg ctaaaaaccc ccgacaagcg ggggttcgaa gaggagttaa tttgccttaa
    48421 gtgtatcagg cgttggcgtc tttgacttcg cggatgatgt cctggtactc gcgcaatttc
    48481 tgcggagtca tcatacggcg ctgatcgtca agcacgacac cgcccacttc atcggcaata
    48541 tgctgcgcag attgcagcat cagcttgaag ttctgcagct cgtcaccgta agacggtacc
    48601 tgcataaaga tagtgacacc cggagtagtg aaatccttca tttcaggatc aaaggttccc
    48661 ggtttcacca tattcgccag gctgaataac gccgggccgc tgccatccgg gctaagatga
    48721 cgatggtaaa tattcatatc gccaaaaatg aagcccgctt gttgaatgct gttaagaagc
    48781 agttcaccgt ttagctcgct accgtgatgc gccgcgacgt tcatgataat caccgcttct
    48841 ttgcgcttcg gtttatccat aactggagcc ggttccgcta caggctcagg ctgtggtgcc
    48901 gctacgggtt ctgcaggctg gaaagcctgt tgtgccggtt gcggtgctga atgcacaggc
    48961 tgcggcgctg gcgcgacctg tggcgaaact ggctgctgca acggctgttc aggctgcggc
    49021 tgataggcag gctgctgcac cggctgcgct ggacgcggag catgttgcgg cggtacctgc
    49081 gcttcaggcg gctgctggac cggttgacgc ggctgcgcag acgcataagg cggtaggtac
    49141 tggtgttgcg gcgacggacg agcagcctca tgctcctgag cgttagccgg ggcatgattc
    49201 acgcggtgaa cacgaacctc accaacgccc tcatcatctt cgacatcctc gtcataagaa
    49261 tcgtcgtcac gttttgactt cattcgtttt aatggccgat cgcggaacat agaagatcgt
    49321 tctttacggc tggtccagaa accatgtacc agtaaagcga ttatggcgat cgcgccaaca
    49381 atgattaata tcagacgcaa atcctgcatc attatattct ctgttgttct aacaccttgc
    49441 caccacggca aacatttact cactaagagt atttgccgat tacctcaagt gcaagtgcac
    49501 tattaacttt cacagcacaa agatagatga aatcgtgctt tttgctgttt tttcgaacat
    49561 atcctaactg tccattgcgc aattacccgg taaaatacgc agaattttcc tgggattggt
    49621 caaaaggagc tcatcctgat tatggtttca tcattcacat ctgccccacg cagcggtttt
    49681 tactattttg cgcaaggctg gaagctcgtc tcgcaacctg ggattcggcg tttcgttatt
    49741 ttaccgctgc tggtcaatat tttgttgatg gggggcgcat tctggtggct ctttacacag
    49801 ctcgatgtct ggatcccgac tttcatgagt tacgttccgg actggctaca atggctgagt
    49861 tatctgttgt ggcctctggc tgtcatctct gtgctgttag tgtttggcta tttcttctcc
    49921 acgattgcta actggattgc cgctccgttt aacggtttat tggctgaaca actggaagca
    49981 cgattgactg gcgctacacc gccagatacc gggattttcg gtatcatgaa agatgtgccg
    50041 cgaatcatga aacgcgaatg gcaaaaattt gtctggtatc tgccgcgcgc aattgtatta
    50101 ctaattcttt acttcatccc tggtattggg caaaccgtcg cgccggtact gtggttcctg
    50161 tttagcgcct ggatgttagc catccagtat tgcgattacc ccttcgataa ccacaaagtg
    50221 ccgtttaaag agatgcgcac cgccctgcgc acacgcaaaa tcaccaatat gcagtttggt
    50281 gctttaacca gcctgtttac gatgatcccg ctgcttaatc tgttcatcat gcccgttgcc
    50341 gtttgtggcg cgacggcgat gtgggtcgat tgctatcgcg ataaacacgc gatgtggcgg
    50401 taacaatcta ccggttattt tgtaaaccgt ttgtgtgaaa caggggtggc ttatgccgcc
    50461 ccttattcca tcttgcatgt cattatttcc cttctgtata tagatatgct aaatccttac
    50521 ttccgcatat tctctgagcg ggtatgctac ctgttgtatc ccaatttcat acagttaagg
    50581 acaggccatg agtaagattt ttgaagataa ctcgctgact atcggtcaca cgccgctggt
    50641 tcgcctgaat cgcatcggta acggacgcat tctggcgaag gtggaatctc gtaaccccag
    50701 cttcagcgtt aagtgccgta tcggtgccaa catgatttgg gatgccgaaa agcgcggcgt
    50761 gctgaaacca ggcgttgaac tggttgaacc gaccagcggt aataccggga ttgcactggc
    50821 ctatgtagct gccgctcgcg gttacaaact caccctgacc atgccagaaa ccatgagtat
    50881 tgaacgccgc aagctgctga aagcgttagg tgcaaacctg gtgctgacgg aaggtgctaa
    50941 aggcatgaaa ggcgcaatcc aaaaagcaga agaaattgtc gccagcaatc cagagaaata
    51001 cctgctgctg caacaattca gcaatccggc aaaccctgaa attcacgaaa aaaccaccgg
    51061 tccggagatc tgggaagata ccgacggtca ggttgatgta tttattgctg gcgttgggac
    51121 tggcggtacg ctgactggcg tcagccgcta cattaaaggc accaaaggca agaccgatct
    51181 tatctctgtc gccgttgagc caaccgattc tccagttatc gcccaggtgc tggcaggtga
    51241 agagattaaa cctggcccgc ataaaattca gggtattggc gctggtttta tcccggctaa
    51301 cctcgatctc aagctggtcg ataaagtcat tggcatcacc aatgaagaag ccatttctac
    51361 cgcccgtcgt ctaatggaag aagaaggtat tcttgcaggt atctcttctg gagcagctgt
    51421 tgccgcagcg ttgaaactac aagaagatga aagctttacc aacaagaata ttgtggttat
    51481 tctaccatca tcgggtgagc gttatttaag caccgcattg tttgccgatc tcttcactga
    51541 gaaagaattg caacagtaat gccagcttgt taaaaatgcg taaaaaagca cctttttagg
    51601 tgcttttttg tggcctgctt caaactttcg cccctcctgg cattgattca gcctgtcgga
    51661 actggtattt aaccagacta attattttga tgcgcgaaat taatcgttac aggaaaagcc
    51721 aaagctgaat cgattttatg atttggttca attcttcctt tagcggcata atgtttaatg
    51781 acgtacgaaa cgtcagcggt caacacccgc cagcaatgga ctgtattgcg ctcttcgtgc
    51841 gtcgcgtctg ttaaaaactg gcgctaacaa tacaggctaa agtcgaaccg ccaggctaga
    51901 ctttagttcc acaacactaa acctataagt tggggaaata caatgttcca gcaagaagtt
    51961 accattaccg ctccgaacgg tctgcacacc cgccctgctg cccagtttgt aaaagaagct
    52021 aagggcttca cttctgaaat tactgtgact tccaacggca aaagcgccag cgcgaaaagc
    52081 ctgtttaaac tgcagactct gggcctgact caaggtaccg ttgtgactat ctccgcagaa
    52141 ggcgaagacg agcagaaagc ggttgaacat ctggttaaac tgatggcgga actcgagtaa
    52201 tttcccgggt tcttttaaaa atcagtcaca agtaaggtag ggttatgatt tcaggcattt
    52261 tagcatcccc gggtatcgct ttcggtaaag ctctgcttct gaaagaagac gaaattgtca
    52321 ttgaccggaa aaaaatttct gccgaccagg ttgatcagga agttgaacgt tttctgagcg
    52381 gtcgtgccaa ggcatcagcc cagctggaaa cgatcaaaac gaaagctggt gaaacgttcg
    52441 gtgaagaaaa agaagccatc tttgaagggc atattatgct gctcgaagat gaggagctgg
    52501 agcaggaaat catagccctg attaaagata aacacatgac agctgacgca gctgctcatg
    52561 aagttatcga aggtcaggct tctgccctgg aagagctgga tgatgaatac ctgaaagaac
    52621 gtgcggctga cgtacgtgat atcggtaagc gcctgctgcg caacatcctg ggcctgaaga
    52681 ttatcgacct gagcgccatt caggatgaag tcattctggt tgccgctgac ctgacgccgt
    52741 ccgaaaccgc acagctgaac ctgaagaagg tgctgggttt catcaccgac gcgggtggcc
    52801 gtacttccca cacctctatc atggcgcgtt ctctggaact gcctgctatc gtgggtaccg
    52861 gtagcgtcac ctctcaggtg aaaaatgacg actatctgat tctggatgcc gtaaataatc
    52921 aggtttacgt caatccaacc aacgaagtta ttgataaaat gcgcgctgtt caggagcaag
    52981 tggcttctga aaaagcagag cttgctaaac tgaaagatct gccagctatt acgctggacg
    53041 gtcaccaggt agaagtatgc gctaacattg gtacggttcg tgacgttgaa ggtgcagagc
    53101 gtaacggcgc tgaaggcgtt ggtctgtatc gtactgagtt cctgttcatg gaccgcgacg
    53161 cgctgcccac tgaagaagaa cagtttgctg cttacaaagc agtggctgaa gcgtgtggct
    53221 cgcaggcggt tatcgttcgt accatggaca tcggcggcga caaagagctg ccatacatga
    53281 acttcccgaa agaagagaac ccgttcctcg gctggcgcgc tatccgtatc gcgatggatc
    53341 gtaaagagat cctgcgcgat cagctccgcg ctatcctgcg tgcctcggct ttcggtaaat
    53401 tgcgcattat gttcccgatg atcatctctg ttgaagaagt gcgtgcactg cgcaaagaga
    53461 tcgaaatcta caaacaggaa ctgcgcgacg aaggtaaagc gtttgacgag tcaattgaaa
    53521 tcggcgtaat ggtggaaaca ccggctgccg caacaattgc acgtcattta gccaaagaag
    53581 ttgatttctt tagtatcggc accaatgatt taacgcagta cactctggca gttgaccgtg
    53641 gtaatgatat gatttcacac ctttaccagc caatgtcacc gtccgtgctg aacttgatca
    53701 agcaagttat tgatgcttct catgctgaag gcaaatggac tggcatgtgt ggtgagcttg
    53761 ctggcgatga acgtgctaca cttctgttgc tggggatggg tctggacgaa ttctctatga
    53821 gcgccatttc tatcccgcgc attaagaaga ttatccgtaa cacgaacttc gaagatgcga
    53881 aggtgttagc agagcaggct cttgctcaac cgacaacgga cgagttaatg acgctggtta
    53941 acaagttcat tgaagaaaaa acaatctgct aatccacgag atgcggccca atttactgct
    54001 taggagaaga tcatgggttt gttcgataaa ctgaaatctc tggtttccga cgacaaaaag
    54061 gataccggaa ctattgagat cattgctccg ctctctggcg agatcgtcaa tatcgaagac
    54121 gtgccggatg tcgtttttgc ggaaaaaatc gttggtgatg gtattgctat caaaccaacg
    54181 ggtaacaaaa tggtcgcgcc tgtagacggt accattggta aaatctttga aaccaatcac
    54241 gcgttctcta tcgaatctga tagcggcgtt gaactgttcg tccacttcgg tatcgacacc
    54301 gttgaactga aaggcgaagg cttcaagcgt attgctgaag aaggtcagcg cgtgaaagtt
    54361 ggcgatactg tcattgaatt tgatctgccg ctgctggaag agaaagccaa gtctaccctg
    54421 actccggttg ttatctccaa catggacgaa atcaaagaac tgatcaaact gtccggtagc
    54481 gtaaccgtgg gtgaaacccc ggttatccgc atcaagaagt aattcttgcc gcagtgaaaa
    54541 atggcgccca tcggcgccat tttttatgct tccgccagcg gcggcaaaat caattcatcg
    54601 ctctcatgct gctgggtgta gcgcatcact tccagtacgc gcaaccccgc acggtgcacc
    54661 gcatcgttta acgccttccc tttcagcaaa ccactgataa gctgagcaca aaacaggtcg
    54721 ccagtccctt tcaggtcggt ttttacccgt gaatgggaaa tgacattcac gctgtcggca
    54781 ctgaccacca ccacctgcat ctcctgattt tcttcattac cggaggcgct ggtaatcacc
    54841 acccatttta atgtgtctga aagcagactt tttgcggcag caatggcact gtcgagatcg
    54901 cggcaatttt taccggtcag gatttccagc tcaaagatat tgggggtaat tccctgcgcc
    54961 agcggcagta aatattgtcg atacgcctcg ggaaggtcag gtttgacata aattccgcta
    55021 tcaatatcgc caatcaccgg atcgaccatg atcaataggt caggatggtc tttgcgtagc
    55081 gcagtcagcc actcggcaag gattttgatt tgcgatgccg ttcccatata gcccgtggtt
    55141 acagcacgaa gttggcgcag cgcatcacgc tcctgaagcg cacgcaaata gccgctaaac
    55201 cattcgtccg gaatcgcacc accgtagaaa gtgtcataat gcggcgtatt gctcagcaat
    55261 accgtcggca cggcaaagac attcaagccg ttctgtttta tagcaggcac cgcaatgctg
    55321 ttgcctacgc ttccgtacac tacctgcgac tgcacggcga cgatatccgc ctgcagcgcc
    55381 ctactcttat cgttaaacaa caacaaacta ctcatttaat tttttctcct tgccgatgat
    55441 cctcatcgta ttccaaccga aactttacct gattctggca gtcaaatcgg ctattacaaa
    55501 acaaagataa ggtaattcag tgaagaaaat catttgtctg gccattacgc tgctaatgac
    55561 actccccgct tacgcgaagt taactgttca tgaagaagcc cgcatcaacg ccatgctgga
    55621 gggattagca cagaaaaagg atttgatatt tgtgcgcaac ggtgatgaac atacctgcga
    55681 tgaagcggtt tcccacctgc gtctgaagct cggcaatacc cgtaaccgca ttgacactgc
    55741 cgagcagttt attgataagg tagcttcgtc gtcatcgatt accgggaagc cgtatatcgt
    55801 gaagatcccc ggtaagagcg atgagaacgc acagcctttt ttacatgcgt taattgcgca
    55861 gacggataaa acggtgcctg cggaaggaaa ttaatccgct ttggtaaggc atttacagga
    55921 ggtaacatga aaaaatgctt tatttatcac gatgaaaaat cgaataaatt ttggtggata
    55981 gattacgaag gggatagttt agctgtcaac tatggcaagg taggtagtat tggtaaattc
    56041 catacaaaag aattcgataa tgaagaacag tgtctgaaag aagccagtaa attgattgcc
    56101 gcaaaaatga aaaaaggata tcaagaaact ccagacttta acttcatgga tctctactat
    56161 tttgatgatg aagagattgg gttacatctt aaaacgtcac acccaaactt ccagtgccat
    56221 tttactgatc cactttatat gtgttgctgg gatgaagaat ctccttttgg tagcgatgaa
    56281 ggtgctgatg ctctaaacga acttgaaaat agcctccgtt ctggactgtg ctgatttccc
    56341 tcaaatgtta attgaaacta tgtggggtat gaaatacatc gctatggaca gtattcttga
    56401 agaggatgtg cgtgcgcaat tactagccga tgaaatgagc tctatccaga gcaatatgat
    56461 tacctacgcg actgcattcg gtcagatcaa agtcatgggt aaaatctccc ataaacttaa
    56521 aaagatggga ctcaatgcac tagcgcgtca tcagcttacc gcaaaaattc ttcaatgggg
    56581 tgacggtcag gactcaccaa tacttcaaaa aatgattgat gaccttactg cttttccaca
    56641 cgaaaattaa aaactgcatt tgtcggtagc aacaactgcc gacaaatcag attattgaca
    56701 aagtgcactt tgtttatgcc ggatgcggcg taaacgcctg atccggcata aaaatattgc
    56761 taattcaata tattgcagaa acattgtagg cctgataagc ggagcgcatc aggcagtttt
    56821 gcgtttgtca tcagtctcca atgctattaa tccttaaatc cctgccccct gactaaaatg
    56881 ctcttcccca aacactccgg tggagaggta gcgatcgcca cgatcgcaga tgatcgctac
    56941 caccaccgca ccagggttag cttttgccac ccgcagtgct ccagcaaccg cgccgcctga
    57001 gctgacgcca cagaatattc cttcccgcac cgccagttcg cgcatggtgt tttccgcatc
    57061 gcgctgatga atatccagca cctcatccac cagcgcagcg ttgaaaatcc ccggcagata
    57121 ttccgcaggc cagcggcgaa tgcccggaat actgctgcct tcttccggtt gcaggccaac
    57181 aatggtcacc ggtttggatt gttcgcgcat aaagcatgaa acgccggtga tagtgccggt
    57241 cgtccccatg ctggagacaa aatgagtgat gcgcccgccg gtttgctgcc agatttccgg
    57301 cccggtggtg gtgtaatgcg cataagggtt atcgggatta ttgaactgat cgagcagctt
    57361 tccttcgcca cgattcgcca tctccagcgc cagatcgcgc gcaccttcca tgccctgctc
    57421 tttggtgaca agaatcagtt ccgcaccata agcacgcatc gccgcacggc gttcctggct
    57481 catgttgtcg ggcatcagca atttcatgcg atagcctttc agcgcggcaa tcattgccag
    57541 cgcaatgccg gtgttaccac tggtggcttc gattaagaca tcacccggtt taatttcccc
    57601 gcgcttttcc gcctcgacga tcatcgaaag tgccgcacga tctttcaccg aacctgccgg
    57661 gttattgcct tccagtttta accacacttc actgccgtta tccggcccca ttcgctgcaa
    57721 cttcaccaga ggcgtattgc ctattgtttg ttctaatgta ctcacaatct ctatccatac
    57781 gtggtgttgc ctgatgcgac gcttgcgcgt cttatcaggc ctacaggtta caaaccttgc
    57841 cattaaaaag cccggatcgc gggaagcgcc tccgggcgtt taacattcac tcaacctatc
    57901 aggcgctttg tgcgagagca agttcctcat cgcgggtttc aatacgctcg tcgccgttat
    57961 acagccgcgc atgttgcaga ccaacgaata aacgctcgcc atgctgcggg gcatcgtcgc
    58021 catgcatcac gaccgtcagc ggttcgttgt accaccccag cggctgcacc actaattggg
    58081 tgtagtgacc tttcgggctg gcttccagta cctgtaccgg cagcggcgaa tcgagactgg
    58141 tacggcggct gatatccact tcccacgggc gcaggaagag atccaccggc ccctgatacg
    58201 caggtgtgta gcccagcggc cagcgatgcg cgccaacatg gaactgcccg ccgcgaatgg
    58261 ttccctgcag gcggttcact tcgcccataa attcgagtac aaaacgggtc gctggttcgc
    58321 gccatacctg attcggcgca tcagcctgtt caatattccc ctgactcatc accactacac
    58381 gatcggctac ttcggtcgcc tcttcctggt cgtgggtcac aaacacgctg gtgaatttca
    58441 gctcttcatg cagttgacgc agccagcgac gcagctcttt acgcacctgc gcatccagcg
    58501 cgccgaacgg ttcatcaagc agcagaattt gcggttctac agccagcgcg cgcgccagcg
    58561 ccacacgctg tttctggccg ccggaaagct gcgccggata acgatccgcc agatgcgcaa
    58621 gctggaccat ttccagcaat tttgtcactt tcgctttgat ggctgcggca ttcgggcgct
    58681 cgcgacgcgg cagcaccgtc aggccaaaag tgatattgtc gaataccgtc atatggcgga
    58741 acagcgcgta atgctggaac acgaaaccga ctttacgatc gcgcgcgtgc aggcgactca
    58801 cgtcggtgcc gtggaagcga atatgcccgc tggtttgatg ctccagcccg gcgataatcc
    58861 gcagcagcgt ggttttcccg gaaccggacg gccccagcaa cgcgaccatc tgaccagaag
    58921 gaatatccag tgagatatcg ttcagcacct gggtgcgacc aaaagacttc ttgatattgg
    58981 caatctcaat gctcatgatg ctcctcctgc tgcgcgcgtt tttcctgatt ctccaggcgc
    59041 cactgcaaca tactctttaa aaacagggtg ataatcgcca tcaacgtcaa cagcgcggcg
    59101 gcggtaaagg agccgacggt gttgtagtcc tgctccaaca attcaatctg taacggcagc
    59161 gaaagtgttt cgccgcgaat cgagccggaa accaccgaca ctgcaccaaa ctcgccaatc
    59221 gcgcgggcgt tggtcagcac cacgccgtaa agcagcgccc agcgaatatt cggcaatgtg
    59281 acgcgacgga acatctgcca gccggacgcg ccaagcaaaa tcgccgcttc gtcttcctgg
    59341 ctgccctggc ttaacatcac cggcaccagc tcgcgcacca caaacggaca cgtcacaaag
    59401 atggtgacca gcaccattcc gggccaggag aacataattt gcagattatg ttcatccagc
    59461 cagccgccga gcgggccgtt ggagccgtag aacagcagat acaccagacc ggcaaccacc
    59521 ggcgatacag caaacggaat gtccagtagc gtcagcagta actggcgtcc cgggaagtta
    59581 aagcgcgtca ccagccaggc cagcagaatg ccgaacacca ggtttaccgg tacggcaatc
    59641 agcgcgatca tcaccgtcag ccagatggcg tgcagcatgt ccggatcggc cagattctgt
    59701 aaaaccggca tcagcccctt gctgaatgcc tgcacgaaga tgtaaatcat cggcaccagc
    59761 aggatgaacg ccgaaaccag catcccgatg ccaatcagaa accatttgcc ccagttaatc
    59821 gggcgcgcgt cataacgctt caattgggta acttccgcca ttaatgacct accacacgcc
    59881 gaccaaagcg actttgcaga gtgttaattg agaacagcag cagcagagat gccgcgagga
    59941 tcaccgaagc aatcgcgctc gctgccgggt aatcaaactc ctgtaagcgc acaaaaatca
    60001 tcagcgacgt cacttccgtc ttccacgcga tatttccggc gataaaaatc accgcgccaa
    60061 attcaccaag actacgggta aacgacagcg ccacgcccgc caccagcgcc ggagaaagct
    60121 ccggcagcac cactttgcag aaactctgcc agcgcgttgc accaagcgtt tccgccgctt
    60181 cttcatattc cgggcctaac tcttccagca ccggctgcac ggtacgcacc acaaacggaa
    60241 tgctggtaaa ggccatcgcc accgcaatac caagccaggt ataggtgact ttgatatcaa
    60301 acttcgccag ccattcaccg taaaaaccgt ttacggaaaa gagcgaggcc agcgttaaac
    60361 cggcgactgc cgttggcagc gcaaagggta aatccatcag cgcatcaagc agcgtgcggc
    60421 ccggaaagcg ataacgtgtt aaaatccacg ccatcagcag accgaaaacg ccgttaaaaa
    60481 tcgatgccac aaacgccgac agcagcgtca ctttgtaggc cgcgaccacc tgcgggttgg
    60541 tgatcacctc ccagtactgc gcccagctca tctcagaaag ctgcatcacc agcgcagaga
    60601 gcggcagcag caatatcagg cacacaaaca gcagactggt gccaaggctt aaagtaaagc
    60661 ccggcagcac gcgtcttgaa gaaactgcaa acattactta cgccccgccg ctaacagctt
    60721 gtctaactcg ccgccgctgg tgaagtgggt tttcatcact tccggccatg agccaaattt
    60781 gtcttccacg cggaacagtt cggtctgcgg gaatttatct ttcagtttgt ccatgacttc
    60841 cgggttattc acacggtagt aatagtcggt gatgatggtc tgcgcctgtg ggctgtagag
    60901 ccagttcagg taggcttttg cggctttttc cgtaccgttg gcctgcacat ttttatcgac
    60961 ccacgccacc gggaattccg ccagaatatt ggttttcggg atcaccactt caaagccctg
    61021 tgcttcatac tgtttacgga tgttgttcac ttccgattca aagctaatca gcacatcgcc
    61081 caagccacgc tcggcaaaag tggtggttgc gccacgaccg ccggtatcga acacttcaac
    61141 gtttttcagg aactgggtca taaactgttc ggttttggct ttatcgccac cgtcagcttt
    61201 atccgctgcg ccccatgccg ccagataggt ataacgcgcg ttacccgacg ttttcgggtt
    61261 cgggaaaatc agcttcacgt cggagcgcac caggtcgttc cagtcgtgga tattcttcgg
    61321 gttgccctta cgcaccagga agcccatggt ggagtagaac ggcgagctat tattcggcag
    61381 gcgcgactgc cagtcagccg ggatcagctt gcctttgtcg tgcaggattt gtacgtcggt
    61441 cacctggtta taagtgacaa cgtctgcttt taagccctgc aaaatcgcca gcgcctgttt
    61501 tgatgacccg gcatgagatt gttttatcgt cagtttgtcg ccgccgttat cttttgccca
    61561 ttgctgttca aacggcggat tcagggcggc aaacagctcg cgggagacgt cataagaact
    61621 gttcagcagt tccgttgcct gtacatggcc cgccagcagc agagaagcga ccagcgcgag
    61681 tgagttcttt ttcagtaagt taacggccat tgcgcaccct tataaattta atgactttct
    61741 aatagccatc atatttataa cggacgtgaa aggagtaacg gttttatata ccgtttggtg
    61801 atttggaagt taaaaaggga ataagaactt ggttcaacag gcgtaattgt tgcaggcagt
    61861 ttggacacgg acagcgcgga gaaaccggag cgtacacata gtacgtgagg atttcgagca
    61921 ctgcccgggg ccaaaatgac aaatgaaata gcctggtgaa cttagttcaa gacgaaatcc
    61981 tccccacaca tgcagggagg aaacagaggt gaatcagata ccgacgctaa ccgtctccgg
    62041 cagtgtgctg ccgccatcaa tcacattctg tgtaccggtt aaatagctgg attcatccga
    62101 tgcgaggaag gccgccagtt cgccgacttc cagcggatcg gcgaggcgac acagcgggat
    62161 tgcttttgcc atttcagtca gcaccgattc tggatcttcc gggttcgact ggcgggcaat
    62221 gctttccgcc attggcgtgc ggacgtatcc cgggcagatg gcgttaacgc gaataccgga
    62281 ttgcgcgtac tccaccgcca gcgatttagt caggccaaca atcgccgctt tcgttaaggc
    62341 atacgccgtt tcgccaggat cagccaccat atcaccagtg actgaagaca tcatcacaat
    62401 gcgaccatct ttgcgcgcaa tcatctccgg cagcaccgcc ttcgtgacgt tccatacgcc
    62461 tttaatattg atatcaatat ggaaatcgcg atcttcatcg ctcatatcga ggaaactgcc
    62521 cagacgacaa acgcctgcgt tattcaccag gatatcaatg cgcccttctt tttccttcgc
    62581 gcgtttgata gctgcggcta ccgacgccgg gtcacgcaca tcggcgacaa ccgccgtaca
    62641 gcgatgacca cgaccacaca gttcgtccgc tagcttttcg atctcagggg agatatccag
    62701 caagattagg ttcgcgccat gacgtgcaaa agttctggca attccttcgc caattccctg
    62761 caatgcgccc gtaatcagtg ctgtcttgcc cgtgagttta cccattttaa tgctctcctt
    62821 gttaatacag agcgttacac tccattccct ctgaaaatac aggataaaca aaatattgct
    62881 gcggatcagc tcactaattc aggaaaatct caatccagtc gctgctttat gcatcatttc
    62941 aggcgttgtg ttagctcact gctgcgctga atcattttca atgacgccac atcattgatg
    63001 ttgcacaaga ccaacaaaca gtaaatcggt aacagaattt tgcgccgtgc gggtagacat
    63061 cgacgagcta cgccattcgg tttcaccgga aacggtatcg agcgtgaaat gcgccagccg
    63121 ccgcagcgga gagtcagcca gagaagtaat ggcaatcacc gttgccccct gctttcgtgc
    63181 cgcttctgcg cacaagacaa tctctttttt actgccgctg taagagatag cgatttgtac
    63241 atcgcctttt ttcaacgcct gggaaacggt ggcttgtacg tgcgtgtcgg cttcgcaggc
    63301 cacgcgatag ccaattttca tcagtttgaa cgacagatcg cgccccacca gcgccgatcc
    63361 gcccaggccc gttatctgga taaacggtgc tttactgatg acctcaatga ttttttgcag
    63421 ccgcgcgtag tcgaacaacg cacaggtttg ctccagcgcc agctcttttt cgcgattcag
    63481 tttgcgggca atcacttcca gcgaatcatc actggtaatc gaactgtgca ggtgcagcgc
    63541 cgtggcattc gttttctccc ggctggcact gtattcgcca attaacgcca tacgcaattc
    63601 agtaaatcct tgcgcgccga gcttctgggc aaatttcaca atgctcgact ggctgatacc
    63661 gagctgtttc gccatctgcc gtgaagagac ggatttcagc tcgctcactc tggcccgcag
    63721 aaagtcagca attttttgct cgttcccggt aaattcactt tccgcattgc gtattttcgt
    63781 caggtacaac atcttccact ctcctaggct ggcagcaaaa attccgctta agttcttatg
    63841 acgctcttca cactctgcga gtgtagactc aatgattcct ttaagtctgt caattcggga
    63901 tatatgattc catacatctg caattatttg atcgtaaata gtaaggtcac caccgatgca
    63961 actagaaaag atgattactg aaggctcgaa caccgcctcg gctgaaattg accgcgtatc
    64021 gacgctggaa atgtgccgga ttatcaacga tgaagataaa accgtaccgc ctgccgttga
    64081 tcgcgtcctg ccggatatcg ccgcggcgat cgatgttatc cacgcccagg tcagcggcgg
    64141 cggtcgtctg atttatctcg gtgcgggaac atccggtcgt ctggggattc tggatgccag
    64201 cgaatgtccg cccacctacg gcgtgaaacc gggtctggtg gttggtttga ttgctggagg
    64261 cgaatatgcc attcagcacg cggtggaagg agctgaagat agccgggaag gtggtgttaa
    64321 cgatctgaaa aatattaatt taacggcaca ggatgtggtg gttggcattg ctgccagcgg
    64381 tcgcacgccg tatgtgattg ccggactgga atacgcacgc cagctcggct gccgcacagt
    64441 gggaatttcc tgtaatccgg ggagcgccgt ttcaaccacc gctgagtttg ccattacacc
    64501 gattgtaggt gccgaagttg ttaccggttc ttcgcggatg aaagcaggta cagcgcagaa
    64561 actggtgctc aatatgcttt ccaccgggct gatgattaaa tccggcaaag tgttcggcaa
    64621 cctgatggtc gatgtggtcg ccaccaacga aaaactgcat gtgcgccagg tcaatatcgt
    64681 taaaaacgcc accggatgta atgccgaaca agcggaagcg gcgttaattg cctgcgagcg
    64741 caactgtaaa acggccattg tgatggtgct gaaaaatctc gatgccgcag aagctaaaaa
    64801 acgcctggat caacacggcg gctttattcg tcaggtttta gacaaggaat aacccatggc
    64861 caaagagatc agcagtgaac ttctgaacac cattcttacc cgtgtcggcg gaccgggaaa
    64921 tatcgccagt tgtggtaact gtatgacgcg cctgcgtctg ggtgtacatg acagttcact
    64981 ggttgatccc aatatcaaaa cgctggaagg cgtgaagggc gtcattttga ccagcgacca
    65041 ggtgcaggtc gtttttgggc ctggtaaagc gcatcgtgcc gcaaaagcga tgagcgagct
    65101 gctgggcgat gcaccggtac aggatgccgc agaaatcgcc gcccagaaca aacgtcagtt
    65161 aaaagccaaa caaacctccg gcgtgcaaca atttctcgcc aaattcgcca ccatcttcac
    65221 gccgctgatc cccggtttta ttgccgccgg tctgctgctg gggatagcga cgttaattgc
    65281 cacggtgatg cacgttccgg cagatgctca gggcacgcta cccgacgcgc tgaattttat
    65341 gaaggtgttc agcaaaggtt tgttcacctt cctagtgatt ctggtgggct ataacgccgc
    65401 ccaggcattt ggcggcacgg gcgtcaatgg cgcaattatt gccgcactct ttttgctcgg
    65461 ttataacccc gctgcaacca ccggttacta cgccggtttt cacgatttct ttggtctgcc
    65521 catcgatccg cgcggcaata ttatcggcgt gctgattgcc gcgtgggcct gcgcccgcat
    65581 tgaaggcatg gtgcgccgct ttatgccgga cgatctcgac atgctgctga cctcgttaat
    65641 caccctgctg atcaccgcta cgcttgcgta cctgatcatt atgccgctgg gtggctggtt
    65701 attcgaaggt atgtcatggc tgtttatgca cctgaacagt aatccgttcg gttgtgcggt
    65761 tttagccggg ctgttcctga tcgccgtggt atttggcgtg catcagggct ttattcctgt
    65821 ttacctcgcg ttaatggaca gccagggatt caacagctta tttcccatcc tttcaatggc
    65881 aggcgcgggc caggtgggcg cggcgctggc actctactgg cgggcgcaac cgcacagtgc
    65941 gctacgcagt caggtacgcg gggcgattat tcccggcctg ctgggcgttg gtgaaccgct
    66001 gatttacggt gtcaccctgc cccgcatgaa gccgtttgtt accgcctgtt taggcggcgc
    66061 ggcgggcggt ttgtttatcg gattgatagc ctggtggggt ctgccgatgg gcttaaacag
    66121 cgcctttggg ccgtctggtc tggtggcgct gccgctgatg acttccgcac aaggcatcct
    66181 tccggcaatg gcggtttatg ctggcgggat tctggtggca tgggtttgcg ggtttatttt
    66241 caccacgctc tttggctgcc gtaacgtcaa tctggactga tattatgaaa cggacaatgc
    66301 tctatctttc tctgctggct gttagttgta gcgtcagcgc cgcaaaatac cctgttctga
    66361 cagaaggtag ttgatcaagt atatgcggcg ttaaagcagt aataaaaagg ccagtcagta
    66421 atgttactgc ctggccttta atcaatgaat cagaaacgct tacagcgcca tcaacttgtc
    66481 cagcgacggt gcgaaataat agccgccggt taccggtttg gtgaaacgca acatcgcatc
    66541 acgtttgcca tcggtatcgc caaacatgct cagcagttgc tgctcaatgt tatgcagacg
    66601 cgcgcagtag gcgcagaagt acagaccgtg agtgccactg gcggtgccgt acggcaggct
    66661 ctggcgaaca atcttcagcc ctttgccatc ttctttcaga tcaacgcggg tgaggtgaga
    66721 ggtttccgga cgttcgtcgc cgtcaatctc ttcgttggcc tctttggtgc gcccgatcat
    66781 catctcctga tcgtgaacgc tcatccggtt gagctgcttc aggttgtgct cccaacgctg
    66841 cacaaacaca tagctgccgc cggaatccac gccgtctttg ataaccgcca cttcgcggcg
    66901 cgtctcttca cccgccgggt tttctgtacc gtcaacaaag ccgctcaggt cacgctcttc
    66961 cacccaacgg aagccgtgga tctcttcttt cacttcaatg cagtcaccaa aggcttccat
    67021 cgccgcctga gcgacagaga agtttacgtc gtgacgcaga gaaagaatgt ggatcaacac
    67081 atcgaactgg gtcgtcggcg caaggccttt accgtagccc ggaaaatctt tcagctcttc
    67141 tgccccaacg ccgccgctca gagcgcgcca ggtgttgtta ccaaaggcaa ccaccgcacc
    67201 aagatgcgcg tccgggaatt tcgcttcaaa agttgccagt ttgtcggcaa atgttttact
    67261 ggccgcacgc agggcgtcaa cttccccttt cacgttggct tcgatccaaa tcgccgcgcg
    67321 gcaatgttct ggcaaaatgc cactctgaac ctgagacatt gttcctcctg aaaataagaa
    67381 tgccacgcaa tccgtggcat tgttgagcgc tattttacgt gttttttcgc gacggcggtt
    67441 tgttcagacg caaattaacg ccgccagata attttactga ctttccagtt tttcagggta
    67501 tcgtccgaag gcattaaccc ttcaggacca cgccattccc cgctgaactg gtagctaata
    67561 tgttgactgc cttcggcttt acattcgacg gcacgattat catcaccatc agctttttgg
    67621 caattgccaa atgctttgct gtaaaggtcg ctaaacggtg taccgatttt aacaccggtg
    67681 tcagcaggaa tatcgctatc cagcacgtca atgcgactga tcgtgccttg atcgccgtta
    67741 atcaccatcg ccacgttgtc gcctttcatc acttcaaaaa agcgcaccac gttgccgttc
    67801 gtggttttca ttccgctgcg caggcgataa tcgccatcaa gcgcatcagc aatggcttgt
    67861 tcttgcagtg gtgtggacgc cgttaattca cccacgccct gctcgctcac tttggtggac
    67921 gatccaaacc agttccacgg gttagcggca gaccagttaa ctgacgacat cgtggaacag
    67981 ccggtcagca tcaacggcat agcgcataac attaaacgca gcgatttcat gtcacttcct
    68041 ttggttattc aataacgttg cttggagtgc aaattcacca aaaagtgccg ttattctttt
    68101 tcttcattaa aacaagcgcg taaacgtcga ttggtcagca gccagattaa cgccacaata
    68161 tccgccacta ccagcgccag accgataccg ctaacggatt caccgttcag ccacagccac
    68221 ggttgccagc aaagtaaaac cacctgcgcc aacagcaaca gaaaatagag cacatgccag
    68281 gtgcgaggaa acgaagcccg ccgaccgctc aacaaaaacg ccagcaccgc cggaataccg
    68341 ggaatcagcc ccagccagaa attatcgtga tcgggataaa acagatttag cagcgcagta
    68401 ccctgctcac gcgacgcacc ggcaatgaca aacagcaccc aggttcgcgc ctgaagcaat
    68461 agcacaagcc agaagagcaa gggtaaacgc aggcgtccgt gcgcatcata atggacagga
    68521 tggaactcag tactcttcat cttcaatcaa acgcttaccc agactcagca cgtcggcgtg
    68581 ttcatatccc aggcgttcat acattccgag caccatgtcg ttatcttccg gcacgttgat
    68641 ctgaattttc gggcagccgc gagcaatcag ctttttctcc agccggttaa gcaacgcatt
    68701 ggcaattcca cgcccacgaa actctggatg cacgccaaga taataagcag acccgcgatg
    68761 cccgtcataa ccgcccatca ccgttccgac cacttcaccg tttacctcag cgaccaaaaa
    68821 caaactgacg tcatggttca tcttacgctc gatgtccatt tccggatcgt tccacggacg
    68881 cagcaagtcg caacgctccc aaagggtgat gacctcttcg aaatcttcct ggcgaaatac
    68941 gcgtatctcc atggtattcg ttaccttttt gcgggttaaa aggctgatta tggcgtgaac
    69001 ggtcgaatta gccaatatct gacgaaaatc ggttgaaaaa gtggcataat ggggagttgt
    69061 caactattga aatgaaaagt aaaacaattc tcaacagcaa accgtcgtaa cggattacgc
    69121 gatacgatat aacatctgaa actttattat tacaactcag gccgtatgag cacttttaaa
    69181 ccactaaaaa cactcacttc gcgccgccag gtgctgaaag ccggtttagc tgccctgacg
    69241 ttgtcaggaa tgtcgcaagc cattgccaaa gacgaacctt taaaaaccag caacggacac
    69301 agcaagccga aagccaaaaa atctggcggc aaacgtgtcg ttgttctcga tccaggccac
    69361 ggcggaattg ataccggagc gatcggacgc aacggttcga aagaaaaaca tgtggtgctg
    69421 gcgattgcta aaaacgtccg ttccattttg cgtaatcatg ggattgatgc gcgtttaacg
    69481 cgttctggcg atacgtttat cccactttac gatcgcgttg aaatcgccca taaacatggc
    69541 gcagatctgt ttatgtcaat tcatgccgat ggctttacca acccgaaagc tgccggtgct
    69601 tcggtatttg ccctctctaa ccgtggggca agtagcgcaa tggcgaaata cctgtctgaa
    69661 cgcgaaaacc gcgccgatga agttgccggt aaaaaggcga ctgacaagga tcacctattg
    69721 cagcaagtac tgtttgatct ggtgcaaacc gatactatta aaaacagcct gacgcttggc
    69781 tcgcatattc tgaaaaagat taagccggtg cataaactgc acagccgcaa caccgaacaa
    69841 gccgcgtttg tggtgttgaa atcgccgtcg gttccttcgg tgctggtgga aacctcgttt
    69901 atcaccaacc cggaagaaga gcggctgtta ggcacggcgg cgtttcgtca gaaaatcgcc
    69961 acagcgattg ctgaaggcgt gatcagttat ttccactggt tcgacaacca gaaagcacat
    70021 tcgaaaaagc gataagttat gaaacccgac gcacaccagg ttaaacagtt tctgctcaac
    70081 cttcaggata cgatttgtca gcagctgacc gccgtcgatg gcgcagaatt tgtcgaagat
    70141 agttggcagc gcgaagctgg cggcggcggg cgcagtcggg tgttgcgtaa tggtggtgtt
    70201 ttcgaacagg cgggcgtcaa cttttctcac gtccacggtg aggcgatgcc tgcttccgcc
    70261 accgctcatc gcccggaact tgccgggcgc agtttcgagg cgatgggcgt ttcactggta
    70321 gtgcatccgc ataacccgta tgttcccacc agccacgcga atgtgcgttt ttttattgcc
    70381 gaaaaaccgg gtgcagagcc cgtttggtgg tttggcggcg gcttcgattt aacccctttc
    70441 tatggttttg aagaagacgc cattcactgg caccgcaccg cccgtgacct gtgccagcca
    70501 tttggtgaag acgtttatcc ccgttacaaa aagtggtgcg acgattactt ctacctcaaa
    70561 catcgcaacg aacagcgcgg cattggcggg ctgttctttg atgatctgaa cacgccagat
    70621 ttcgaccact gttttgcctt tatgcaggcg gtaggcaaag gctacaccga cgcttattta
    70681 ccaattgtag agcgacgtaa agcgatggcc tacggcgagc gcgagcgcaa ttttcagctc
    70741 taccgtcgcg gtcgttatgt cgagttcaat ctggtctggg atcgcggcac gctgtttggc
    70801 ctgcaaactg gcgggcgcac cgagtcgatc ctgatgtcaa tgccgccact ggtacgctgg
    70861 gagtatgatt atcagccaaa agatggcagc ccagaagcgg cgttaagtga gtttattaag
    70921 gtcagggatt gggtgtaact ccctcacccc cattcccgca tccgctgatg caacgtcaac
    70981 gacggcttct cggcaaacag ctgctggtaa tccgtggcaa attgccccag atgccagaat
    71041 ccccactgca tggcggcgtc ttttaccgtt gtgctttgcg accacgggct tatcagttcg
    71101 cggcgtacga cgttcaggcg aatgcgtttc agccacgcgt ttgggccaat gcctaaaata
    71161 gcgtgaaacg cgttttgtag cgtgcggcga ctgacatgca gttgattaca caaatccagc
    71221 accgtcaccg gttcggacat gttttccagc acatattcac gggcgcggga aagcaaccga
    71281 cggtaactct gatgactgat gctttccgcc gtcaccattg gttgcgcttc ttccagcatc
    71341 gcccccatcg ccattagcaa attatccccc agcacttttc gcactgcagg ctgatggaga
    71401 ttttccgggt tctcgcaaaa cgtcgccagc gcctgttgga caaagcccca cagcgcggct
    71461 ttatgctgct ctttcacttc cagcgccgac tggctacgca gcatatgtaa tacccgatcc
    71521 ggattgtgca aaaagttagc ctgacaggtg atgacatctt cagacagcac tacgcccagg
    71581 atcgtataat cgtccggcgt acttaattca aactcggttc caccggggcg cgtcgctatt
    71641 tctgcgcttc ccagacattg cgaaccgata aatccctgct caccgcgcgt cgccggaatg
    71701 ccaaaccaga acgagttcgg ccagaccagg cacgactgac gcagcgccag accggtgtat
    71761 tcacgaaaaa cctgaatatc atcgagtaga atttccgtaa attcaccatg aaacttgccc
    71821 ggatgcagct gatcgtaaat ctgctgctag gcggtaatcg tcaaagcatg ttcatagaca
    71881 tccgttgtct gtcgttgatg aacattatcc acttcgacct tcggcgtgag cttcaggttt
    71941 tcgggtaagg gttcatgata aagatggtgc aaattggctg tacgggtctt tttcatgatg
    72001 ttaatgccgg gtgttgtagg acacccggca cctccgacag gttaatgggg cttgagacga
    72061 taacgactac tgcgtttacg taacgtcccg gcagaaaaga gctgttcgag cgcatttctg
    72121 gctttttcca gcggccagcc aaagtgggcg gccacttctc ctgccgtcat tccctgacat
    72181 actgatgtta acagcgccaa caactcgtca gcagattccg cgacgataac tggcgcgtcg
    72241 ggttccttta cgggttgctt cggctggcgg ttaaagccag taaccagcca ctgggtgtca
    72301 tcgtctggcc gaccgatctc cttacggctg atgacacgcc cggtacgcat cgcggcagca
    72361 caacctgcgt ccagcgccgc acgacacgcc gccagatcgc cttccaccac cagcgttaag
    72421 cgaccagggt caagcacttc gtgactgagc agacgaacgt tagctgcttt gagcatggca
    72481 tccgctgcat ctatcgcggc gaccatgccg tccacttcca gcaatcccag tgcattgatc
    72541 atcggcaacc tccgttacgc acgctggatt ggattacgcg cgatttccag cactgcatcg
    72601 gtaaaggcgt tacaggctgc tttacacgcg gcctggctac cggttaaaaa tgctgccgag
    72661 tagttggttt cagacggtgg cgggacgtag gtcaccagct gtacgtcggc agatttcaac
    72721 gcggcatcaa tgccgtaggt cgcttccagc ggcggtgcca ccagatacgc catcggatcg
    72781 ccaagcgtga tcccggcggt tgatgaaaga taagaaccgg tacgcgaaac cacatgcgcc
    72841 aggaaggcgg tgttttctgc gtcattggcc cactggaaag ctgcgccatt ttcaatatgt
    72901 gcaaccatcg catccagacc ggcacgcact tccgccgggt ttggcccacc aagcataatc
    72961 agcacttcac cggcagtcgg tgacgggccg tgtgccgctc ccgcatacag cgagcgacca
    73021 tataccactt cgaccatcgc ttgtttggtc gcttcgtcag ccgcaatgta ggttacgtcg
    73081 tcagaatcgg cagaaatcag cccgaggcta cgaatatgcg gcggcaattt tagttcacgc
    73141 gcaaattcag cgttaacgga ggcaatcacc cgcatggcgg ttaccgacgg tcgaatcaaa
    73201 tctaaagctg gcatgatgcc tccttatcgg gtcatgttga tgccggatgc tttctgctcc
    73261 agcatacgtt tcgccaaatc cacaatgacg gcacagcagc acgaaagaga agcaaaagtc
    73321 agctacctgg tacactaggc agcgggtcag caaagggggg caaatgcccc ttttttcatg
    73381 tcagataatc agatcattac ctcaataaac taataatcac atcgcggtta caaccagttt
    73441 gatagtgata gctcaaaccc agacgtggcg ctggctgcaa gcaaaataag ccgcaaaaat
    73501 gcgaccccat gtttactagc cgcaaaaatg cggtcctatt tgccagaacc ttttattatt
    73561 caattggtta gtgcaagaaa aagcgtgatt actgttcaag aagtatccag aaaggtcatg
    73621 attttgacgc tgaaaaaaaa taggtgggca tttttgccca ctcactagcc gcaaatttgc
    73681 ccactcacta gccgcatttt tgcggcccaa agtaataaat acagagaaat aataaataca
    73741 gatcagcgcc aagatttttc gtcttttcgg cggaatcatg agactagtgc ttacgtcctg
    73801 ataacctcca gagcagacct taacttctgt gcagcaggac tacgctcatt gccgctcggc
    73861 taggtcaaat cctcacgata aagctgttcg gattgtgacc ataatgttaa aggactcttt
    73921 aaatcattcc ttgacgaatt aactagctgg cttaagcgga atagcatgca ggataaaaac
    73981 aaaaccagct tagcagtggg gagagcttcg cttcgctcgt ctcctggggc caatgctcaa
    74041 tgaaacgacg taaggcacgg agcgctcacc ataggtagac acataaacat ctgagagcgc
    74101 accttctatc gcgctacgcg ctcaataaaa accaacgaca ccgccacact gcaagaccaa
    74161 tggtttttat cgcgtataca gaccagacag gaaacagcag caaagcagcg tatagccagg
    74221 tgcagacagc acactcacac gacgcacagc aggcttaagt gatcactcag gaaactttat
    74281 ccccgccgcc gatcaccgcg aactatcatg accgcgccaa attcgaaagg aatggcccat
    74341 agaacgcgat gaacacacat aagcgctaca cttacttaac gtcgccacag agcgattgag
    74401 agcgcttaaa atcgatgcga tttcattaaa tttttctcac tcgcctgaat ggtggcgatc
    74461 aggactgatc gcaacattgc cccgcctcga tattgagcgc ataatagata catacattaa
    74521 gcggaaaaga gataagttta ttgtgaaaat cagggggtta catatcatgc gcatatcaga
    74581 acaacaacgg cagatcctcg actatctggc aaaaaacgaa tcaatcaccg cacgacaagc
    74641 cgcagatctc gtttatggtg gcaatgttac ccgcgtccag gttgacgccg cccgccgctc
    74701 actcatgacg atggttagta atgggctaat caggaaacaa ggccgcgctt tcgtagctga
    74761 aagacatatc aatcacgctt atagccagga cgttgaagaa ttgcgccgcc agatcctttg
    74821 ggtattcagc aaacagggtc aggagtattt cctttctgaa cacccaaatt tcgccgccaa
    74881 agagttaaca gtaagcggat tgtcgtgcgc actggattat cttgtcggtg aaggttgcgt
    74941 tacttacaag cagctatggc acgccctgaa tacgcttgta gaagatggtt tactcattgc
    75001 cacgcgtgag aacatcgaat actggataat gccgcgttca ggtcgccgca atcatgcacg
    75061 gtatatttca ggcaacagat acagaacgcc ggactttaat ccgacacagg aagatcggga
    75121 ccgtgccgaa atgatgtgca gggaacagga gaaaagaatt atggataagt tattttcgat
    75181 gcagtgaaca caagcgggcg caatgcccgt ttttattgcc ttattccacg cctgacgaat
    75241 caatttgatc acacctgaga aaatgcatag ccagaagtta aggccgttat attgcgattg
    75301 ggagcgtttt aaacggtatc aatttaatta attctgatgt ggtgatttgc aggcaaaaaa
    75361 aacccgccgg agaagcggga aatagagaca tatcgttgta gttattaaca gatcactttg
    75421 aggtcgaggg cggctaaccc tcgctaatat tatcccgcta ttaccttgcg ggtgcaaata
    75481 atcttttatt gctttgatga tttaataaat agcacttttt gctaacaaaa accgctgtca
    75541 atatatggcc aaaaatgtca acagaaaaaa taaggtttat aatattaagt atcacatggg
    75601 taatgggttc agggaatcgg aatccaccgc ctcacggaaa ttcaaaattt gcttcttctt
    75661 tcctccgtgg gcggcttctt tttcgtatcg aggtatttat gaaagcaata acattagaac
    75721 agttcgcagc aatggcgaat gtatcgacca gaacgctgag gcgctggcta aaaaaaccat
    75781 caaccggagt ccctttgcca tttaccccac gcggtactcg ccaattattc tgggagcacg
    75841 aatgcaaaga atggttgtac agaacagaac gataataatc acgcatcaca ttttgaggta
    75901 aacatcatga ccaatatcga aagattagaa cgctgtgccg atattatgcg acgcagatgg
    75961 atttatgacc ctgaagcggg tttattgttt agccgtgaaa caaaagacgt tatcaaaggc
    76021 agcatgacca ataaagggca tttggttgta acggtacatg aaggtaaatt caaagtgaca
    76081 ctcacctatc agaaagcctg ctatgtatac acatacggcg cttatgacga gacattatat
    76141 gaagtgcacc acgttaattt gaacaaacag gataaccgca tcaagaatat tcgcctgatg
    76201 ccgaaagaaa agcaccgccg gatacataaa aacgtccgca aacttattcg gctgggccac
    76261 acacaatggc ttcaaccgca attaattgaa caatcactat aaacacagga gtaaatacca
    76321 tgcattcatt agacagttat tttcagcgca cgacagcccc taaatcagcc gcccaggaac
    76381 gccgtgagga attccatgag aaagtgatgc gttctgctga ttatattgcg gacaagttcg
    76441 tggaaacggt tcgtccactt gttgatgaag tagctgataa acttcagtct gaaatgccgg
    76501 aagatatgga aggcactgca aagcggcgac tcatttgcga attaagtcgt cgttttggcg
    76561 tgtcaataag cgcttttaaa taatcgaaaa aaggggggaa agataatgga cgcatttgag
    76621 gtactggcaa ataaaggcgc tgaactggta gcacagcgtg ataaaaccgc caacgaaggg
    76681 gaacgcgcac tgctaaataa acagattaaa gcgatccgta tggcccagtt taaattaatc
    76741 agtaatgaag ttatcgaaac aattaaaccg cagatcccac aaatcgttgc tgacgctatt
    76801 aaagccgccg gactggagaa acgcattgcc gggctaaaaa cgaacgcaga gaaaatggag
    76861 atgtacaaac agagcggtgc tccgaatccc gatgagtact tctcaccgga tgttgagttt
    76921 atggcgcagg ttgaggaacg tctgggtagc tgtctcaccg aggaacagcg tcgctatttt
    76981 gatggagtgg atagttcggc aggtattgac ctgaactcgt atttcggtcg tgaaattgag
    77041 cattttgatg ccgccccgtc tatgccggag ccagaaccag aaatggccac cgatcctgaa
    77101 gctgagcaac ggaaacgcgt attcaatgcg atagggattc ggtactgatc ggataaaaga
    77161 aagccgggaa ttaccccggc tgacttaaca atgtgaccaa tgaacaaatc ggacaagaca
    77221 aacaggataa gcagtctcga aacaatgttc gggaactata ttagcacaag tccggagaaa
    77281 atcatcatgt tggcactacg catcatgcag ggcatcgcta agaccctcgc agaacatgtc
    77341 ttagacctga aacattcgcc actgagcaaa caggccatga agcgccaaac gcttcgtctg
    77401 tgggctgaat attcgcttgg aactatcaat aaaatcatcg acatgaaatc ggggccgagc
    77461 aaccagagcg cggaggaaat ggagttcatc aggcgtttaa ttctcattcg cagagacatt
    77521 catagccagt tacacagcgt gggaatcgat attaatgatg ggacgggcga ttaattttcg
    77581 gacaagtact atgcgcgcgc gaggagctga acatgaagca agatattgca gacaggctgg
    77641 agatactcga aggccagcgg gcagaagcga aacaactacg taagcaggcc agaagagcgc
    77701 acaggaacaa cgaagcagag ttactaacca aatacatcag tttcaccaat tactgcatct
    77761 acgagtgtta caaggaagat gcagaggact ggctggattc attgccagaa cagtactgag
    77821 cagaataaca ccacgtcatg ttgttctgag gtgagcatat cacctctgtt caggtggcca
    77881 aatttagtgt gccacttcag catcaccgtt gaaatcacaa taacgatttt tttgtaaata
    77941 tgctctcaca tatgtaggaa tagaccaggt tggcattgaa acacatctac catgaccata
    78001 ataaagtaat tttttgatat gtttatggtt ttatattaat taaaatatat ctaaatttga
    78061 tagttttaaa attgcaaaat ccagattgtg tttgtaatgt tt
//
LOCUS       DAZPAB010000002        70712 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZPAB010000002 DAZPAB010000000
VERSION     DAZPAB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.002"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..795
                     /locus_tag="ACHUPG_000084"
     CDS             <1..795
                     /locus_tag="ACHUPG_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0859866.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="ACHUPG_000085"
     CDS             806..1897
                     /locus_tag="ACHUPG_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0859867.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="ACHUPG_000086"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="ACHUPG_000086"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR0859868.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..4901
                     /gene="lpoA"
                     /locus_tag="ACHUPG_000087"
     CDS             2865..4901
                     /gene="lpoA"
                     /locus_tag="ACHUPG_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR0859869.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4859..5254
                     /locus_tag="ACHUPG_000088"
     CDS             4859..5254
                     /locus_tag="ACHUPG_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR0859870.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5274..5864
                     /gene="diaA"
                     /locus_tag="ACHUPG_000089"
     CDS             5274..5864
                     /gene="diaA"
                     /locus_tag="ACHUPG_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR0859871.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5874..6449
                     /gene="dolP"
                     /locus_tag="ACHUPG_000090"
     CDS             5874..6449
                     /gene="dolP"
                     /locus_tag="ACHUPG_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR0859872.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6563..7603)
                     /locus_tag="ACHUPG_000091"
     CDS             complement(6563..7603)
                     /locus_tag="ACHUPG_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR0859873.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7676..8311)
                     /locus_tag="ACHUPG_000092"
     CDS             complement(7676..8311)
                     /locus_tag="ACHUPG_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR0859874.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8439..8957
                     /gene="yhbO"
                     /locus_tag="ACHUPG_000093"
     CDS             8439..8957
                     /gene="yhbO"
                     /locus_tag="ACHUPG_000093"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR0859875.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8937..9380)
                     /locus_tag="ACHUPG_000094"
     CDS             complement(8937..9380)
                     /locus_tag="ACHUPG_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR0859876.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9431..9733
                     /gene="yhbQ"
                     /locus_tag="ACHUPG_000095"
     CDS             9431..9733
                     /gene="yhbQ"
                     /locus_tag="ACHUPG_000095"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR0859877.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9720..10223)
                     /locus_tag="ACHUPG_000096"
     CDS             complement(9720..10223)
                     /locus_tag="ACHUPG_000096"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0859878.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10217..10741)
                     /locus_tag="ACHUPG_000097"
     CDS             complement(10217..10741)
                     /locus_tag="ACHUPG_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR0859879.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10950..11945
                     /locus_tag="ACHUPG_000098"
     CDS             10950..11945
                     /locus_tag="ACHUPG_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR0859880.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11954..12832
                     /locus_tag="ACHUPG_000099"
     CDS             11954..12832
                     /locus_tag="ACHUPG_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR0859881.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12913..13920
                     /locus_tag="ACHUPG_000100"
     CDS             12913..13920
                     /locus_tag="ACHUPG_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR0859882.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="ACHUPG_000101"
     CDS             complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="ACHUPG_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR0859883.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="ACHUPG_000102"
     CDS             complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="ACHUPG_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR0859884.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="ACHUPG_000103"
     CDS             complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="ACHUPG_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR0859885.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="ACHUPG_000104"
     CDS             complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="ACHUPG_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR0859886.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="ACHUPG_000105"
     CDS             complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="ACHUPG_000105"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR0859887.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="ACHUPG_000106"
     CDS             complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="ACHUPG_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR0859888.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21298..22242)
                     /gene="truB"
                     /locus_tag="ACHUPG_000107"
     CDS             complement(21298..22242)
                     /gene="truB"
                     /locus_tag="ACHUPG_000107"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR0859889.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="ACHUPG_000108"
     CDS             complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="ACHUPG_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR0859890.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22807..25455)
                     /gene="infB"
                     /locus_tag="ACHUPG_000109"
     CDS             complement(22807..25455)
                     /gene="infB"
                     /locus_tag="ACHUPG_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR0859891.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="ACHUPG_000110"
     CDS             complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="ACHUPG_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR0859892.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSAELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="ACHUPG_000111"
     CDS             complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="ACHUPG_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR0859893.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27654..27730)
                     /locus_tag="ACHUPG_000112"
     tRNA            complement(27654..27730)
                     /locus_tag="ACHUPG_000112"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27694..27696),aa:Met,seq:cat)
     gene            28078..29421
                     /gene="argG"
                     /locus_tag="ACHUPG_000113"
     CDS             28078..29421
                     /gene="argG"
                     /locus_tag="ACHUPG_000113"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR0859894.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29429..31055)
                     /locus_tag="ACHUPG_000114"
                     /pseudo
     CDS             complement(29429..31055)
                     /locus_tag="ACHUPG_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(31514..31600)
                     /locus_tag="ACHUPG_000115"
     tRNA            complement(31514..31600)
                     /locus_tag="ACHUPG_000115"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31564..31566),aa:Leu,seq:gag)
     gene            complement(31615..31947)
                     /gene="secG"
                     /locus_tag="ACHUPG_000116"
     CDS             complement(31615..31947)
                     /gene="secG"
                     /locus_tag="ACHUPG_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR0859895.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="ACHUPG_000117"
     CDS             complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="ACHUPG_000117"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR0859896.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33505..34353)
                     /gene="folP"
                     /locus_tag="ACHUPG_000118"
     CDS             complement(33505..34353)
                     /gene="folP"
                     /locus_tag="ACHUPG_000118"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR0859897.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="ACHUPG_000119"
     CDS             complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="ACHUPG_000119"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR0859898.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="ACHUPG_000120"
     CDS             complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="ACHUPG_000120"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR0859899.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37232..37525
                     /gene="yhbY"
                     /locus_tag="ACHUPG_000121"
     CDS             37232..37525
                     /gene="yhbY"
                     /locus_tag="ACHUPG_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR0859900.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37681..38157)
                     /gene="greA"
                     /locus_tag="ACHUPG_000122"
     CDS             complement(37681..38157)
                     /gene="greA"
                     /locus_tag="ACHUPG_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR0859901.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38405..39838
                     /gene="dacB"
                     /locus_tag="ACHUPG_000123"
     CDS             38405..39838
                     /gene="dacB"
                     /locus_tag="ACHUPG_000123"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR0859902.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            39902..40003
                     /locus_tag="ACHUPG_000124"
                     /pseudo
     CDS             39902..40003
                     /locus_tag="ACHUPG_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="ACHUPG_000125"
     CDS             complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="ACHUPG_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR0859903.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41212..42177)
                     /locus_tag="ACHUPG_000126"
     CDS             complement(41212..42177)
                     /locus_tag="ACHUPG_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0859904.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="ACHUPG_000127"
     CDS             complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="ACHUPG_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR0859905.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="ACHUPG_000128"
     CDS             complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="ACHUPG_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR0859906.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43151..44122
                     /gene="ispB"
                     /locus_tag="ACHUPG_000129"
     CDS             43151..44122
                     /gene="ispB"
                     /locus_tag="ACHUPG_000129"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR0859907.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44349..44627
                     /gene="sfsB"
                     /locus_tag="ACHUPG_000130"
     CDS             44349..44627
                     /gene="sfsB"
                     /locus_tag="ACHUPG_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR0859908.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44675..45934)
                     /gene="murA"
                     /locus_tag="ACHUPG_000131"
     CDS             complement(44675..45934)
                     /gene="murA"
                     /locus_tag="ACHUPG_000131"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR0859909.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="ACHUPG_000132"
     CDS             complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="ACHUPG_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR0859910.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="ACHUPG_000133"
     CDS             complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="ACHUPG_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR0859911.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="ACHUPG_000134"
     CDS             complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="ACHUPG_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR0859912.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="ACHUPG_000135"
     CDS             complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="ACHUPG_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR0859913.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="ACHUPG_000136"
     CDS             complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="ACHUPG_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR0859914.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="ACHUPG_000137"
     CDS             complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="ACHUPG_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR0859915.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49715..50680
                     /locus_tag="ACHUPG_000138"
     CDS             49715..50680
                     /locus_tag="ACHUPG_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR0859916.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50694..51680
                     /gene="kdsD"
                     /locus_tag="ACHUPG_000139"
     CDS             50694..51680
                     /gene="kdsD"
                     /locus_tag="ACHUPG_000139"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR0859917.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51701..52267
                     /gene="kdsC"
                     /locus_tag="ACHUPG_000140"
     CDS             51701..52267
                     /gene="kdsC"
                     /locus_tag="ACHUPG_000140"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR0859918.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52264..52839
                     /gene="lptC"
                     /locus_tag="ACHUPG_000141"
     CDS             52264..52839
                     /gene="lptC"
                     /locus_tag="ACHUPG_000141"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR0859919.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52808..53365
                     /gene="lptA"
                     /locus_tag="ACHUPG_000142"
     CDS             52808..53365
                     /gene="lptA"
                     /locus_tag="ACHUPG_000142"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR0859920.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53372..54097
                     /gene="lptB"
                     /locus_tag="ACHUPG_000143"
     CDS             53372..54097
                     /gene="lptB"
                     /locus_tag="ACHUPG_000143"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR0859921.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54145..55578
                     /gene="rpoN"
                     /locus_tag="ACHUPG_000144"
     CDS             54145..55578
                     /gene="rpoN"
                     /locus_tag="ACHUPG_000144"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR0859922.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55601..55888
                     /gene="hpf"
                     /locus_tag="ACHUPG_000145"
     CDS             55601..55888
                     /gene="hpf"
                     /locus_tag="ACHUPG_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR0859923.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56006..56497
                     /gene="ptsN"
                     /locus_tag="ACHUPG_000146"
     CDS             56006..56497
                     /gene="ptsN"
                     /locus_tag="ACHUPG_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR0859924.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56543..57397
                     /gene="rapZ"
                     /locus_tag="ACHUPG_000147"
     CDS             56543..57397
                     /gene="rapZ"
                     /locus_tag="ACHUPG_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR0859925.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57394..57666
                     /gene="npr"
                     /locus_tag="ACHUPG_000148"
     CDS             57394..57666
                     /gene="npr"
                     /locus_tag="ACHUPG_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR0859926.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57879..58511
                     /gene="yrbL"
                     /locus_tag="ACHUPG_000149"
     CDS             57879..58511
                     /gene="yrbL"
                     /locus_tag="ACHUPG_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR0859927.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="ACHUPG_000150"
                     /pseudo
     CDS             complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="ACHUPG_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="ACHUPG_000151"
     CDS             complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="ACHUPG_000151"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR0859928.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="ACHUPG_000152"
     CDS             complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="ACHUPG_000152"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR0859929.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62547..63476)
                     /locus_tag="ACHUPG_000153"
     CDS             complement(62547..63476)
                     /locus_tag="ACHUPG_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR0859930.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64151..68611
                     /gene="gltB"
                     /locus_tag="ACHUPG_000154"
     CDS             64151..68611
                     /gene="gltB"
                     /locus_tag="ACHUPG_000154"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR0859931.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68624..70042
                     /gene="gltD"
                     /locus_tag="ACHUPG_000155"
     CDS             68624..70042
                     /gene="gltD"
                     /locus_tag="ACHUPG_000155"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR0859932.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70226..70630
                     /locus_tag="ACHUPG_000156"
                     /pseudo
     CDS             70226..70630
                     /locus_tag="ACHUPG_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
BASE COUNT        17316 a        19176 c        17559 g        16661 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccaccatta ccgtcatggt gcggtaaaag aagacgttgc gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata
     3721 tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac
     3781 aacctcagcc gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc
     3841 tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg
     3901 cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac
     3961 aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc
     4021 cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg
     4081 cactgaacca gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac
     4141 cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc
     4201 tggtgctgat cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt
     4261 ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac
     4321 gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag
     4381 cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg
     4441 accagttcac caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg
     4501 aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc
     4561 tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg
     4621 aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc
     4681 aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg
     4741 atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca
     4801 acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat
     4861 ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga
     4921 tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc
     4981 taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt
     5041 ttttgtcgag gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac
     5101 ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg
     5161 gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga
     5221 gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag
     5281 aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc
     5341 ttccggatgc catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca
     5401 aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca
     5461 tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata
     5521 atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg
     5581 tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc
     5641 gcgatattgt taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga
     5701 ccggctatga cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca
     5761 ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt
     5821 gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg
     5881 cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg
     5941 ccgtagtggg taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca
     6001 cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac
     6061 agattaagaa agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg
     6121 ggcagtcacc aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg
     6181 gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat
     6241 ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga
     6301 aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga
     6361 ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg
     6421 ttaccacagc gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg
     6481 ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc
     6541 atccgacatg ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag
     6601 acactgccac catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca
     6661 taatcagtga cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg
     6721 ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga
     6781 caaacaagca tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac
     6841 catcggcatg ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgt
     6901 aaaccgggat cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac
     6961 cgctctgtgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcggt gtttcacgca
     7021 cccatttttg caccagcgtc gcaatcagca acaccatcac cagcccggcc accagacgaa
     7081 tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca
     7141 ccggattgcc catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc
     7201 ccgccgcgac cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc
     7261 ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga
     7321 tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga
     7381 ggaaatagat catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa
     7441 ggatagattt accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt
     7501 gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc
     7561 actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata
     7621 agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt
     7681 cagctctttt gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca
     7741 tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg
     7801 gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc
     7861 ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt
     7921 cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag
     7981 cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta
     8041 ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc
     8101 cgagacaaca aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt
     8161 gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag
     8221 caatggcgtt aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc
     8281 ccgttgcgcc tgtaatcagt acctgactca tcttcgttcc tttacagaat tgtctgcctt
     8341 gcgctccccg gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg
     8401 aaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt
     8461 taatcactga tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag
     8521 ccggacacga agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag
     8581 gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg
     8641 ccctgttgct accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca
     8701 cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc
     8761 agctgctgat cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca
     8821 ttattgatgt taaaaatgcg ggcgtggaat tttacgatca ggaagtcgtg gttgataaag
     8881 atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac
     8941 gcctgctcgg tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg
     9001 tcggtgaatt tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca
     9061 accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg
     9121 cggatctcac ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt
     9181 accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc
     9241 gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag
     9301 agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg
     9361 atggcgatga gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc
     9421 gatgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta
     9481 taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc
     9541 gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg
     9601 ttcgctggca ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg
     9661 tctggtagcg gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat
     9721 taaaagcgat tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca
     9781 tccgctaaac gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg
     9841 tgatgcgccg ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc
     9901 actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc
     9961 agcccctgtc cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg
    10021 tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca
    10081 tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc
    10141 gcttccgcat cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg
    10201 ggaatttcta ctcgaattag catggttcac ctacagatgt ctgtttggtt tcaggcgcgg
    10261 ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg
    10321 gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat
    10381 cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg
    10441 caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc
    10501 tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat
    10561 gaatacttaa ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga
    10621 attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta
    10681 ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca
    10741 cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag
    10801 cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc
    10861 ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg
    10921 ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc
    10981 cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg
    11041 ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg
    11101 tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc
    11161 cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg
    11221 cgctgatcct cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg
    11281 aacgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc
    11341 gccattttga cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac
    11401 agctggcacg ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgtatta
    11461 tgtcggaagg ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg
    11521 gtgcgtgttc tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc
    11581 gcctgaacga agtgctgatc gaccgttatc aggacggcga aaacgcaggt tatccgacgc
    11641 tgtgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca
    11701 gtctcaatac cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa
    11761 ttgaaggccg ccagcgtagc ccggcgtatg tcagccaggc ggcgaaagtc tggcgtcagg
    11821 ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga
    11881 cgctcgggtc gatgtccgaa ggcacgcaaa ccacccttgg cgcgtatcac cgtaaatggc
    11941 agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac
    12001 gctggaagat ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga
    12061 agcggtatgc agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc
    12121 gctcgccgga agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc
    12181 tgaactgggc gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga
    12241 tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct
    12301 gaactgctac aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg
    12361 tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct
    12421 gggcattcgc aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta
    12481 ctccgcccgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg
    12541 ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt
    12601 actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc
    12661 catgcaggga ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat
    12721 gctcgacgcc ttccgcgcta atgaaaatgg cgctgtgcca ctgccgttga cggcaaacag
    12781 cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag
    12841 ctcactttgt taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca
    12901 aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata
    12961 cccgaaggct cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct
    13021 gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc
    13081 agtgctgcaa catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg
    13141 gggtctggcg gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc
    13201 acactcaata cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt
    13261 gaccaacgga caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc
    13321 cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc
    13381 ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt
    13441 gcacaactgg cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat
    13501 atgttgttcc aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa
    13561 aaaccatacg cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa
    13621 tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa
    13681 ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg
    13741 cagcaggcgc tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag
    13801 tcgatcctgc gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac
    13861 caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag
    13921 tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata
    13981 agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta
    14041 ctgatacacc ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc
    14101 aaacaccaga atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc
    14161 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    14221 cgctaaacca gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac
    14281 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    14341 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc
    14401 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
    14461 gcttaatgct tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc
    14521 cggacgaggg atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat
    14581 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
    14641 caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag
    14701 caaatacggc gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac
    14761 atgccccagc aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    14821 cgccgtcatg cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc
    14881 aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg
    14941 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat
    15001 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    15061 actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    15121 acagaaccag gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac
    15181 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    15241 aagcagcgac ggtgacgttt gggtggtggt tagtgttgcc atgagggctt ctctccagtg
    15301 aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac
    15361 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc
    15421 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
    15481 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    15541 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    15601 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt
    15661 catcggcttg ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc
    15721 tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct
    15781 gctgatgtcg ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc
    15841 gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg
    15901 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    15961 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    16021 ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc
    16081 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    16141 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    16201 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    16261 acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt
    16321 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    16381 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    16441 acgaccgtct ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    16501 cagcgcggcg ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt
    16561 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    16621 tgcttcgttt ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt
    16681 agtcacgctg gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    16741 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    16801 cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    16861 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    16921 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    16981 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    17041 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    17101 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    17161 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    17221 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    17281 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    17341 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    17401 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    17461 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    17521 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    17581 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    17641 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    17701 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    17761 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    17821 aagtgctgtt tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    17881 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    17941 tgcgctaact tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag
    18001 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    18061 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    18121 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    18181 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    18241 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    18301 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    18361 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    18421 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    18481 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    18541 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    18601 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    18661 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    18721 aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    18781 tctgcagtga tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct
    18841 gcaatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg
    18901 gcacggataa cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg
    18961 atggtatgga tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    19021 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    19081 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg
    19141 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    19201 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    19261 ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa
    19321 gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    19381 atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    19441 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    19501 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    19561 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    19621 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    19681 ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    19741 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    19801 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    19861 taagcatcgc tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    19921 accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg
    19981 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    20041 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca
    20101 accagatcca gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca
    20161 ttgatgtaac ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac
    20221 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa
    20281 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc
    20341 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg
    20401 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    20461 gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg
    20521 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    20581 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    20641 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    20701 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    20761 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    20821 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    20881 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    20941 aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    21001 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    21061 gctacctgaa cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    21121 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    21181 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    21241 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    21301 cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    21361 cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc
    21421 accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    21481 caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    21541 cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt
    21601 cacaatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc
    21661 gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga
    21721 gcagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac
    21781 ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt
    21841 tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa
    21901 agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat
    21961 ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    22021 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa
    22081 catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    22141 ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa
    22201 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    22261 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    22321 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    22381 cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    22441 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    22501 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct
    22561 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    22621 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    22681 tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc
    22741 gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca tggaacccta
    22801 aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga tcacatcgcc
    22861 agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc
    22921 gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt
    22981 gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc
    23041 tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat
    23101 ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag
    23161 gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc
    23221 agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt
    23281 ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc
    23341 agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt
    23401 caggacgatg ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag
    23461 tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc
    23521 aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac
    23581 gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc
    23641 cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc
    23701 cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag
    23761 gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac
    23821 ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga
    23881 cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag
    23941 ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac
    24001 caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc
    24061 gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat
    24121 tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc
    24181 aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt
    24241 tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac
    24301 aaccggcgcg cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc
    24361 cagctcgttt tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc
    24421 ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt
    24481 gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc
    24541 gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga
    24601 agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc
    24661 agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc
    24721 acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg
    24781 agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt
    24841 gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt
    24901 acgacgcgct tcttcttcag ctttacgctt gagctctgca gcttcctgct cacggcgggc
    24961 tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc
    25021 cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg
    25081 ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc
    25141 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    25201 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    25261 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    25321 caaagtctgt ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc
    25381 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    25441 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    25501 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat
    25561 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    25621 ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg
    25681 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    25741 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    25801 catcggcaca taggccagtt cttccagcgt cgagaaacct tcttctacca gaacagtcgc
    25861 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    25921 ctgatgctta gcctgcaggt cgtcaacggt cattacgttg agttcccaac cgctcagctg
    25981 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccagcttc
    26041 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    26101 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    26161 ctcgccgccc agttcagcag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc
    26221 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    26281 gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg
    26341 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    26401 ttccgggcga acggaataaa gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    26461 cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat
    26521 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    26581 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    26641 cgtctgggtg gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    26701 caagttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    26761 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    26821 gatctgtaca cggacgtcga tctcttgctc atattttttc tttgttgctg tcgccagcgc
    26881 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    26941 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    27001 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    27061 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    27121 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    27181 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    27241 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    27301 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg
    27361 aataaattcg atgcctacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    27421 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    27481 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    27541 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    27601 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    27661 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct
    27721 ccaccccgcg cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg
    27781 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    27841 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    27901 tgaatacttt tgccttcccc tgctctccct taagcgcatt attttacaaa aaacacacta
    27961 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    28021 ttcagtgaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    28081 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt
    28141 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    28201 actgcaaacc tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    28261 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    28321 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    28381 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    28441 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    28501 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    28561 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    28621 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    28681 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    28741 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    28801 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg
    28861 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    28921 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    28981 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    29041 gcgcatggtc gtcagttggg ccgtctgctg taccaggggc gttggtttga ctcccaggcg
    29101 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    29161 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    29221 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    29281 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    29341 tatgccaaaa ctggcctgct ttcctcctct gccacttcag gcctgccgca ggtggagaat
    29401 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt
    29461 gcttatcctc tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat
    29521 ccacaacccg tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt
    29581 ccggctttgt tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga
    29641 tattgttttc cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca
    29701 tcgggacatg atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct
    29761 tcgtagggtc acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt
    29821 ttaataattc aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc
    29881 aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag
    29941 ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct
    30001 gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt
    30061 ccatggcgcg catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg
    30121 agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt
    30181 aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg
    30241 cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt
    30301 caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag
    30361 actcccccac aatcaacacg taggtatcaa tacttgtatc cctgactgat aattgaaaat
    30421 acggcaccgt gtttgcaatt gagagtaatc tttgatgcgc tttcgctgct aaagcaaaat
    30481 agttgagatt gaaaaacggc gtataggtag caaatcacga cgctagtata tatggactga
    30541 acgctttttt attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg
    30601 aaatgacaat cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt
    30661 ttattattac tgccaaaaaa agaagggata aaaaagcaaa agcacataga taagggatat
    30721 acatccccag cattttcaca acttcatccg gatcactctg cagcacacta atcgcaaatc
    30781 catcattgaa agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg
    30841 cagtcagtaa cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt
    30901 taacaaccag gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gctccggaag
    30961 aggcgactaa gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt
    31021 taaaatcttc tagcaaattt gttgaatact gtcatccaga aatccaagtt cacaaccaat
    31081 ataaacaatt atcaacaatg ttcaatttta tatcagacat gtttttcaac tctgatccat
    31141 atcagaccga aacactgtta gttttttctt tttaaatgat gcgaccattg cgttctcagt
    31201 aattaaataa cattatcaat gcgtctctaa acattacgca gaacgtagcc atcagtagtt
    31261 atactgacag gagcaggatg gtttatcgag aagtaaacat atccgtattc gtaccggaac
    31321 tggagttgac acaataaagt gccaattatg tcagcagtag ggaaaagttt ataactaagg
    31381 cgtaccggca ccatcgtttc aaggtaccag ctacgagtaa agcaactgga cgagatacag
    31441 atacctgaca accattcatc agaccaggac caaaacgaaa agagacgctt ttcagcgtct
    31501 cttttctgga atttggtacc gaggacggga cttgaacccg taagccctat tgggcactac
    31561 cacctcaagg tagcgtgtct accaattcca ccacctcggc acggatacta ctttttagtt
    31621 cgggatatcg ctggtcggct tagccggagc agctggctga gtttgttcgg ttttcgccgg
    31681 tgcactcaga ttttcccatt cgctacctct attggttttg ttgctattga tgttacccag
    31741 caccagactg atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg tcatgaagtt
    31801 accagaacca cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg aggctcccat
    31861 atcagcgcct ttaccttgct gcagcatgat cagaccaaca aggccaattg ccacaataag
    31921 gaaaactact aaaagagctt cgtacataat caacctgttc cttgcggagt tgccgcgtac
    31981 caatgcttca accaataaag cgggagtttt taatctttcc cactgaagcg ggtgtgaata
    32041 ctaaccaaaa cgaatgacct tcgcaagggc aattttatcg cattgtatca actgcggaaa
    32101 aaaacagcaa aaccgatgtg ttcgccgaga aaaaaggcga caatcgccgc ctttttagcc
    32161 agttatctaa cgctttaaac ggcttttact gcatcggcga tgcggtgtgc aaattcagtc
    32221 acctgcgctt cgtcttcgcc ttccaccatc acgcgaatta acggttcggt gccggattta
    32281 cgcagcaaca cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt cacggcttta
    32341 actgactcat gctcaagcgg atcgccgcta cctgcggtgt aacgtacgtt aaccagaatc
    32401 tgcgggaaca ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt acgtgccatc
    32461 gccgccagca cctgcaagcc agcaacgatg ccgtcaccgg tagtggtttt atccagcagg
    32521 atcacatgac cggaattctc tgcaccgata cgccagcctt tctcctgcat tttttccagt
    32581 acgtagcggt cacccacttt cgcgcgcgca aatggaattc ctaactgttt cagcgccagt
    32641 tcaagcccca tgttgctcat caatgtaccc acagcaccac cacgcagctg gccctgacga
    32701 agaccttcac gcgcgatgat atacatgatc tgatcgccat cgactttatt gccttcatgg
    32761 tcaaccataa tcacgcgatc gccgtcgccg tcgaaggcaa taccgagatc cgctttttca
    32821 gccagcacac gagcctggag cgcgcgaacg tcggtagccc ccacttcggc attgatgttt
    32881 acaccgtttg gctcacaacc gatagcgata acgttcgccc ccagttcgcg cagcacgttc
    32941 ggcgcgatgt gataagtcgc accgtttgca caatccacca caatcttcag ttcactgagg
    33001 ctaagttcgt tcgggaacgt ggctttgcaa aactcgatat agcgacccgc ggcatcaacg
    33061 atacggctgg ctttacccag ttcagccgaa tcaacgcagc tgatctcctt ttccatttcc
    33121 gcttcgatgg cctcttctac cgcatccggc agtttagtgc cgtcgataga gaagaattta
    33181 atcccattat cgtagaacgg gttatgcgat gcagatatca caattccggc ctctgcgcgg
    33241 aaggtacgcg tcagataagc cacggccggt gttggcatcg ggccagtgaa gagtgcggaa
    33301 aggcccgctg ccgccagacc cgcttccagt gctgactcca gcatatagcc agaaatacgc
    33361 gtgtctttac caataataat cttacgggag ccgtggcgcg ccagcacttt acccgcggcc
    33421 caacccagct taagcacaaa atcaggtgtg atcggcgcat cccctacacg accacgaatc
    33481 ccatcggtac cgaaatattt acgattactc atagcgtttg ttttcctttg cagacagagt
    33541 ggcttccacc acccgcatcg cttctacggt ttctttgacg tcatgaacac gaatgatgtg
    33601 cgcgccttgc attgcggcaa tgaccgcaca ggccagacta ccgctcaggc gctcggacgg
    33661 ccccacgttc agcagctgcc caatcatcga ttttcgtgac atacccacca acagcggcag
    33721 gttgaaatgg tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg agagattttt
    33781 accgaaaccg aatccggggt cgagcaacaa tttctctttt gcgatacccg cctgctcgca
    33841 acgtgctatt tgctcaataa agtagcgatt cacttctgca aagacatcgt catacttcgg
    33901 agcttcctgc atggtttttg gatttccctg catatgcatc agacaaaccg gtaaaccggt
    33961 ttctgcagcc gcctccagag cgccaggttc ggaaagggag cggatatcat taataatgtg
    34021 agcgccaact ttcgctgact cacggatgac ttctggtttg gatgtatcga ccgagatcca
    34081 gacttcgaag cgttgagcaa ttgcctcaac cacaggaata acacgttgca actcttcttc
    34141 aacgctaact tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc
    34201 gccagcattg atcatcagat tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc
    34261 accatccgaa aaggaatcag gcgtgacgtt gaggatcccc attacgtgag gatggctaag
    34321 gtccagtgaa gtaccctggg caaagagttt cattatgtta tccctggtat gaattgataa
    34381 gaaaaaaccc cggagcacgc cccggggttt tcggtacaaa tacagtcatc tgatgcggga
    34441 acttacttgt cgcctaactg ctctgacatg gtgttacccg ggttcggcgt acgcggttca
    34501 tcaaccggac gaggagcctt tggactacca ttgtcgccag aattgttaga agcgcctggt
    34561 tcttcccagc ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt
    34621 gcgtcgatag tctcatattt catgagagca tctttcatcg catgcagaat atccatattg
    34681 tcggtcagaa gctgacgcgc acgattatag ttacgctcaa tcagtgcttt cacttcctgg
    34741 tcgatgatac gtgcagtttc atcggacata tgtttcgctt tcgctacgct acggccgagg
    34801 aatacttcac cttcttcttc cgcgtacagc agtggaccca atttctcaga gaagccccac
    34861 tgagtcacca tgttacgtgc caggttggtc gcaactttaa tatcgttgga cgcaccggta
    34921 gaaacatgtt ccggcccgta gatgatctct tctgccagac gaccaccgta cagcgtagaa
    34981 atctggcttt ccagtttctg acggctggcg ctgattgcgt cgccctcagg caagaagaaa
    35041 gtcacaccca gcgcacgacc gcgtgggata atcgtcactt tatgcaccgg atcgtgttcc
    35101 ggcaccaggc gaccgataat cgcatgaccc gcttcgtggt aagccgttga ttctttctgc
    35161 gcttccgtca tcaccatgga gcgacgttcc gcacccatca tgattttgtc tttcgctttc
    35221 tcgaactcaa ccatcgacac aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg
    35281 ttcaccaagt tcgccaggtc agcaccggag aaaccaggag taccacgagc aatgattgcc
    35341 gcgtcgatat cgggtgccaa tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga
    35401 ccgcgaacat ctggcaagcc gaccacaacc tggcggtcga aacggccagg acgcagcagg
    35461 gccgggtcga gaacgtccgg acggttagtc gcggcgataa cgatgatacc ttcgttacct
    35521 tcgaagccat ccatctcaac cagcatctgg ttcagagtct gttcacgttc atcgtgacca
    35581 ccgcccagac cagcgccacg ctggcggcct acggcgtcga tttcatcgat aaagatgatg
    35641 cacggtgccg ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc acccacaccg
    35701 acgaacattt ctacgaagtc agaaccggag atagtaaaga acggaacttt cgcttcgcct
    35761 gcaatcgctt tcgccagcaa cgttttaccg gtacccggag gaccgaccat caggacgcct
    35821 ttcgggatct taccgccgag tttctggaag cggctcggct cgcgcagata ctctaccagt
    35881 tcagcaactt cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc
    35941 tgatcttccg tcagcatgcg cgctttgctc ttaccaaacg acatggcacc tttgccaccg
    36001 ccgccctgca tttgacgcat gaagaagatc cagacaccaa tcagcaacag catcgggaac
    36061 caggagatga agatagaagc cagcaggctt ggttcttcag gcggttcacc gacaaccttg
    36121 acgttcttgg tcaacaggtt atccagtaat ttcggatcct gaaccggaat gtaagtggta
    36181 taacggttac tatctttctt ggtaacgttg atttcacgtc cgttgatacg cgcttcacga
    36241 acctggtcgt tattgacctc ttgtaggaag gtagagtaat ccaccttacg gccattagac
    36301 tcgctgggcc caaagctctg gaatactgac atcagcacaa cggcaatgac cagccagagt
    36361 attaggtttt tcgccatgtc actcaaggga ttaacctctt attacaactg tgttaaaaac
    36421 agcgtcagga tactctatat ccagcatctt tcaaactttc gtctgaaatc tcccggttag
    36481 ggtttacgcc cggtcgctac aatatacact tctcgcgaac gtgcacgaga agagtccggc
    36541 ttacgaactt tgaccttcgt aaacagggag cgaatttccc ttagatactc atcgaaacct
    36601 tcgccctgga acaccttcac tacaaaactg ccacctggcg ctaatacatc acgacacatt
    36661 tctagcgcca gttccaccag atacatggct cgggggatat ccaccgccgg tgttccgctc
    36721 atgtttggtg ccatatcgga catgacaacc tggactttgc tgtcgccaac gcgctccagc
    36781 agtgctttca tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac accaacgata
    36841 ggatccatag gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc
    36901 acatattgtg accaaccacc cggagcagca ccaaggtcga caaccgtcat tcccggttta
    36961 aagagtttgt cactttgctg tatttcatca agtttaaacc aggcacggga acgtaatccc
    37021 tttttctgtg cctgttgaac atatttatcg ctaaagtgtt cctgaagcca gcggctggag
    37081 ctggcagaac gcttcttacc tgtcatttaa ctttcccatg gggataactc atcgtaacca
    37141 attgcgtaaa tttttacgcg cctatttggt gatatatggg agatggcggt agaatgaccc
    37201 gttttcaatc ccaacgtaag caaaaaatac gatgaatctg agtactaaac aaaaacagca
    37261 cctgaaaggt ctggcacatc cgctcaagcc agttgttctg cttggcagta atggtttgac
    37321 cgaaggggtg ctggccgaga ttgaacaagc gttagagcac catgaactca tcaaggtgaa
    37381 aatcgccacc gaagatcgcg aaacaaaaac cttgatcgtg gaagctatcg tgcgcgaaac
    37441 cggcgcctgt aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc caactaaaga
    37501 acgtaaaatc tcgctgccac gctaagatta tcctaaagtt acacacattt gctgtgtaaa
    37561 acgaggggtt ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt gataaaaggc
    37621 cgcagagcgg cctttttcct ttctttacaa tacatcaaca tcttgagtat tgggtaattc
    37681 ttacaggtat tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac
    37741 cacaacatca tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat
    37801 caggttttgt ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc
    37861 agaatccaga ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat
    37921 tttggtgaca tcaatcacct gcgcgtttga cagcttggct tcgatgtctt tgatacggcc
    37981 ttcgcagaag ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc
    38041 atgctcacgc gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa
    38101 atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg
    38161 ttatacctct tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg
    38221 cccggcataa ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc
    38281 aggtcagcta caattcacat tttgatggtc attttaccct gaagttcccg aagggtcatc
    38341 gtttacttta tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga
    38401 gattatgcga ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca
    38461 ggccgcgaat gttgatgagt acgttactca actccccgca ggtgccaacc ttgccctgat
    38521 ggtgcaaaaa gtcggtgcgt cggcccccgc tattgattac cacagtcagc agatggcgct
    38581 gcccgccagt acccagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga
    38641 ttttcgtttc accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg
    38701 tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat
    38761 ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac
    38821 ctccattttc gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg
    38881 ctttagcgct ccgcctgctg ccgccatagt tgaccgcaac tgtttctccg tctcgctcta
    38941 cagtgcccca aagcctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac
    39001 gatgttcagc caggtacgca ccctcccccg tggttctgcc gaagcgcaat actgcgaact
    39061 ggatgtggtg ccaagcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc
    39121 tgagccgctc ccgttggctt ttgccgtgca ggatggagcc agctatgccg gtgcaattct
    39181 gaaagatgag ttaaaacagg cgggtatcac ctggagcgga acactgctgc gccagactca
    39241 ggttaacgaa cctggaacgg tagttgccag taaacagtcg gccccgctgc acgatctgct
    39301 taagattatg ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatcgg
    39361 ccatgcgcgc ttcaatgtgc ctggaacatg gcgggctggg tcggacgccg tgcgtcagat
    39421 cctgcgccag caagccggtg tcgatattgg taacaccatt attgccgatg gttcagggct
    39481 ttcgcggcat aacctgattg cccccgccac catgatgcag gtgctgcaat acattgccca
    39541 acacgacaat gaacttaact ttatctccat gctgccgctt gcgggctatg acggctcttt
    39601 gcagtaccgt gcaggtctgc atcaggcggg cgtggatggc aaagtctcag cgaaaaccgg
    39661 ttcgttgcag ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat
    39721 ggcatttgta caatatcttt ctggctatgc agtaaaaccc gcggatcagc gtaatcgccg
    39781 tattccgtta gtgcgttttg aaagccgttt gtacaaagat atttatcaga acaattagtc
    39841 aaaaagaaac cccggcgcat ggccggggtt tcagattatt gacaaagtgc gctttgttta
    39901 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat cgtgcaaatt caatatattg
    39961 caatcatctt gtaggcctga taagtgtagc gcatcaggct tatttggcat tggtcattag
    40021 tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcgtcatc
    40081 ccagtcttca tcatcctctt cagcaatctc ttcaagctgc tgacggtgat aatcatccca
    40141 catgaattcg actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat
    40201 gataaaggtc atcacatccc agcagagatc tttcacgccc agtccgctcg ccgcagagat
    40261 cagataatat ttatcttccc agcccagcgc ttcagcgatc gctttcgctt tctcttcggc
    40321 ttctgcctta tccagcagat cgatcttgtt gaagactaac caacgcggtt tcgccgccag
    40381 atcctggctg tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt
    40441 gccgtcaatc ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt
    40501 caggaagcga atgcccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc
    40561 ggcaacaacg aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt
    40621 ggtaaacgga taatccgcca ctttcggttt agccgccgat accgcacgaa taaaggtcga
    40681 tttacccgcg tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag
    40741 cagctcacgc ttatcgcccg gcgtaccgtt agttttctgc cgtggtgtac ggttaactga
    40801 cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg
    40861 accgtgttta gtcatatcac ccatggtttc accagtaccc tggtcgatta cacgcgtacc
    40921 taccggcact ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc
    40981 gttctgaccg cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag
    41041 gttctcgtcg gcttccatcc atacgtcacc accatcaccg ccgtcgccgc catccgggcc
    41101 gcctttcgga atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc
    41161 aacgaccaga atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc
    41221 tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc
    41281 aatggcggaa tacatcgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt
    41341 taacatcggt ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag
    41401 cgtaaacagt ggggtgagcg tgatgatcgc gctcacctgc gctgcctgcc agcgagccat
    41461 cgcttccgcc aaggcgccat atcctaccaa ggtattcagt ccgcaaaaaa ttaaacatgc
    41521 gagctgccag tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcgat
    41581 tgtacataaa gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac
    41641 cttttgcgcc acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc
    41701 ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac
    41761 cagaccgctc aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag
    41821 gataaatacg ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc
    41881 ggtcggactc aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc
    41941 ggtcgccact gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa
    42001 cctcttcttc accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa
    42061 cacgattgtc ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc
    42121 ccagcaaatt gctgtggtaa gcgccaaaag aatgccaatg cctgcctgct gcttcatacc
    42181 gtattccctg caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta
    42241 tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa
    42301 aacttattca gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt
    42361 cactttaccg tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc
    42421 agcgtggaat ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc
    42481 accgaaacgc ttaacgccca agcgtttagc ttctgaatcg cgaccgttac gtgtggagcc
    42541 gccagccttt ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta
    42601 attttcacat cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga
    42661 cgaaacttaa cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt
    42721 acgccgccat caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc
    42781 acttcagcga actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc
    42841 tgaccttcgc ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac
    42901 tccgcttccg cgcacacctt ttcaatgatt cagagtgcgc tataaatatt cacaataggg
    42961 cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa
    43021 aaacacaact tgtacggtaa cgtttatctg tgccattttt tcagtacaat taccctatat
    43081 tcctaaccat aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc
    43141 ggcttttgcg atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt
    43201 taatgcggca atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta
    43261 ttacatcgtc agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg
    43321 agctgttggc tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca
    43381 cacggcgact ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc
    43441 taccgccaac gccgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac
    43501 ccgcgcgttc cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga
    43561 agctgtaaac gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga
    43621 catcactgaa gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc
    43681 tgccgcgcag tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca
    43741 ggattatggg cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa
    43801 tgccgatggc gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac
    43861 gctgccgctg ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac
    43921 cgccatcgaa caaggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc
    43981 ttgtggatct cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc
    44041 agcgttacag gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat
    44101 cgctgttcaa cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgc gggatgattc
    44161 cagtaaattc cataaaaaca ctaattcagc gctataacac ctgaaaaaag ggcgatgaat
    44221 ctttatatat tctgaatatt cacactcttt acaggaactt tttagcgcaa tagaccatca
    44281 ggagtatagt gatgcccgac agaagaagtg ttctgaatga aagcgactaa cttaaggagt
    44341 gaggaaagat ggaaagtaat ttcattgact ggcatcccgc tgacatcatt gcaggtttgc
    44401 gcaagaaagg aacgtcaatg gcggcggaat ctcgcagaaa tggtttgagt tcctcaacgc
    44461 tggcaaacgc attatcgcgc ccatggccga aaggagagat gattattgcg aaagccctgg
    44521 gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc catgagttta
    44581 tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt
    44641 agccccgcga acggggctgc cagctctcag acgattactc gcctttcaca cgctcaatat
    44701 ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa
    44761 tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg cttgctgatg
    44821 cacgcagatc ggttgccata acctgtgcac cagaaagttt ttcaacacca tgacaaataa
    44881 cggtattgct ttcgatttcg gcgtgcgcgc ccatacggct cagctctggc acatgcataa
    44941 agcggttttc aaagaccgtt tcggtgatga acccggtccc ttcagccacc aggttcaaca
    45001 gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta cgtacgttaa
    45061 cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg acttcgatgt
    45121 ccgctccagc atcacgcagt ttcgccagca cggcgtccag agtatctggc tgcgcgttac
    45181 ggcagataat tttgccacga gagatcgccg ccgccaccag gaaagtaccg gtttcgatac
    45241 gatccggcag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg atgacgatac
    45301 gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc gcggtatcga
    45361 cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt gccttccgcc agggttgcag
    45421 cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata tgtgcgcctt
    45481 tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg gtcgcgccta
    45541 attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta caaccgccag
    45601 gtagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagcgcc cagatagaag
    45661 cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa
    45721 tatgcacaga accattacgt tctactttcg cacccagctg gcttagcagt ttcattgatg
    45781 tatcgacgtc tttcagtttc gggacgttct ggatctctac cggttcttcc gccagtagtg
    45841 cggcaaaaag aataggcaga gcagcatttt tagcgccgga aattgtgact tcgccctgga
    45901 gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt taacaattca
    45961 tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc
    46021 actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc gcaatatatt
    46081 ccatcagcgg gccatagacc gtctgctgtt ttttaacccg actcataccg tcaaacaact
    46141 cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact tcctggaggg
    46201 agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct tcaatcatca
    46261 gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata agcaaaaagc
    46321 ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca ggcggttaac
    46381 gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa ttatacaatt
    46441 tcgcgagggt atacactttg tcgtttaccc cctgaagcgt cacgttgttg ccctgctttt
    46501 tcgccagatc gataagatgg agcagcagtg ccagtccccc cgtatccacg cgggacacac
    46561 ggctaagatc gatgcaggta atccccttca ccgcctcctc acgcatttcc caaagcggta
    46621 gcagaacgtc ctgatccagc tctccggata acgccagcgt gtcacccgtc tgcatccagc
    46681 tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag aaatcgattt
    46741 cagttgcgca gtcaggccgt cgataccttt ggtacgcagc agcgttcccc actcgttttg
    46801 tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcttgcc agtttcccgt
    46861 ctgggagttt ttacgccact ggaagtccag acgcaccggc ggacggccat tcgggtcaat
    46921 aatggtaacg cgaataggca caatggtttt atcgcccagc ggctgttctg gcgcaatctg
    46981 ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa gcctgcttca ggtactcacg
    47041 gaaagcggca aagtaggctt cacgttgagc aggggtcgca ctcttgtaat actggcccag
    47101 caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat caacaatggt
    47161 acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc gatcgaacgt
    47221 tttctgcgcc gcctcgtcca tcagcttata aggattggtc tggtctgccg cggttgccgc
    47281 actcagaggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt
    47341 ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac ctggcgcagc
    47401 agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa ggaactgacc
    47461 aatgagatct tccagcacca tcgcagactt ggtgtcctga attgtatcgc catccttcag
    47521 gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat attgttctcc
    47581 cagcaggccg gaagtacgaa tgctcagcga actggtatca ggaatgtggt tataacgttg
    47641 ttcaatttcc agcgttacgc gcggcagata ggttttcggg tccagcgtaa tatccgccac
    47701 ccggcccaca acaacaccac caatgctgac cggagagcgc gctttcaggc cgccaatgtt
    47761 atcgaacgtc gcataaagcg tgtaggtcga ttcagtacgt atggacgtca cgttcgccgc
    47821 cttcaggcaa acaaacagcg ccgccagcag tgctgctaat aaaaagatac ccacccaaat
    47881 ttcatttttt ttcgtttgca tgaactcaat tcccaaacat caatgcggtc agcacaaaat
    47941 ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc cggctaatcc
    48001 cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc accgtgatgg
    48061 cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg acggcatttt
    48121 gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg accagcgatc
    48181 cgccccagat ccccacggcg acgaagataa ccgtcaacag tggtaatgaa ataaccccag
    48241 cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc tccatactgg
    48301 agagttgctc tgtagcgcgc atcaggccga tttctgcggt taacgccgaa ccagcacgcc
    48361 cggcaaacaa cagcgcggca accaccggcc ccagttcacg cagtagcgat aacgccacca
    48421 gcatacccag actggtttcc gcgctataag tggtcagaac cagataacct tgcagcccca
    48481 acaccattcc gatgaacacg ccagaaacta caataatcag catcgacagg acgccgacat
    48541 tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg ccgaccagcg
    48601 cattgaataa cattaacccg gcccgcccga acgttctcag ggttttaatc cctttatgtc
    48661 cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa aagatcagcg
    48721 tgataatcgc cggcaggata gcggaacgga acaggcccgt cagctatccc gtccagaaac
    48781 tgacgtacgc gcggatcagg attcgtctgc aacgcctggg cactgccatg agcgacaatt
    48841 tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg cacatcgtga
    48901 gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag cttcaccagt
    48961 acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat gagatccggc
    49021 tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga aagttcagaa
    49081 ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat caccgtacta
    49141 tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac gttgtcaaat
    49201 acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg tttacgcact
    49261 gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag gatctcacca
    49321 tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc gatgcccgat
    49381 ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt atcgaagatg
    49441 cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc cacagactgc
    49501 tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa tttaaccttg
    49561 aacccaacat atttacagaa tattacccgc cgtggttagc gaaagctggc atttgtttta
    49621 ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa aatcttcagc
    49681 ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg cactgttaat
    49741 tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg cgtctattct
    49801 ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca gtattggtac
    49861 atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac gcgatttagc
    49921 cgttggtaca gccctcggct caaacattat caatatattg ctgatcctcg gtctggctgc
    49981 gctggttcgt ccttttaccg tccattccga tgttctgcgc cgtgaattac ccttaatgtt
    50041 gttggtcagc gtggtggccg gttccgtact ctatgacgga caacttagtc gaagcgatgg
    50101 tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac ttgcacgtca
    50161 ggggactgac agcctgacca gagagcagct tgcagagctg ccgcgtgacg gcggattgcc
    50221 cgtcgcgttt ttatggctcg gcattgcgct tatcatcatg ccagtggcca cgcggatggt
    50281 ggttgataac gccacggtgc tggcgaatta ctttgccatc agcgagttga cgatggctct
    50341 gacggcaatt gctatcggaa ccagtctgcc ggaactggca accgcaatag cgggggttcg
    50401 caaaggtgaa aacgacattg ctgtcggaaa tatcattggc gcaaacattt ttaatattgt
    50461 catcgtgttg ggtttacccg cgctgataac gccaggagag attgatccac tggcgtacag
    50521 tcgtgactac agcgtgatgt tgctggtgag cattattttt gcgttgctgt gctggcggcg
    50581 ctccccgcaa ccgggccgtg gtgtaggggt attattaact ggcggattta tcgtatggct
    50641 ggcgatgttg tactggttat cgccaatact cgttgaataa ctggaaacgc attatgtcgc
    50701 acgtagagtt acaaccgggt tttgactttc agcaagcagg taaagaagtc ctggcgattg
    50761 aacgtgaatg cctggcggag cttgatcaat acatcaatca gaatttcacg cttgcctgtg
    50821 aaaagatgtt ctggtgtaaa gggaaagttg tcgtcatggg gatggggaaa tcggggcaca
    50881 tcgggcgcaa aatggccgca acgtttgcca gcaccggtac accttcattt ttcgtccatc
    50941 ctagtgaagc cgcgcatggt gatttaggca tggtcacccc acaggatgtg gtgattgcta
    51001 tctctaactc aggtgaatcc agcgaaatca cggccttaat tccagtgctt aagcgtcttc
    51061 acgtaccgtt aatctgcatc accggtcgcc cggagagcag catggcgcgc gccgcagatg
    51121 tgcatctgtg tgttaaagta gcgaaagaag cctgtccgtt agggctggca ccgaccagca
    51181 gcaccaccgc cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg
    51241 gctttactgc tgaagatttt gcgctctcac acccaggcgg cgcactgggt cgtaaacttc
    51301 tgctgcgcgt aaacgatatt atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg
    51361 ccagtctgcg tgacgcattg ctggaagtta cccgcaaaaa tcttggtatg actgtcattt
    51421 gcgatgacaa tatgatgatt gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg
    51481 atatgggcgt ggatgttcgt cagttaagta ttgccgatgt gatgacgccg gggggaatac
    51541 gtgtgcgccc tggcattctg gccgttgagg cactgaactt aatgcagtcc cgccatatca
    51601 cctccgtgat ggttgccgat ggcgaccatt tactcggtgt gttacatatg catgatttac
    51661 tgcgtgcagg cgtagtgtaa agattcaagg ataaacaaca atgagcaaag caggtgcgtc
    51721 gcttgcgacc tgttacggcc ctgtcagcgc cgacgttatg gcaaaagcag agaacattcg
    51781 tctgctgatc ctcgacgttg atggcgtact gtcagatggc ctgatttata tgggcaataa
    51841 cggcgaagag ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac
    51901 ctctgatatt gaagtcgcta tcattaccgg gcgaaaggct aaactggtag aagatcgttg
    51961 tgccacattg gggatcactc acttgtatca ggggcagtca aacaaactga tcgcctttag
    52021 cgatttgctg gaaaaattgg cgattgcccc ggaaaatgtg gcttatgttg gcgatgatct
    52081 catcgactgg ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc
    52141 actgttgatc ccgcgcgccg attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg
    52201 cgaagtttgc gacttattac tcctggcgca gggcaaactg gatgaagcca aagggcaatc
    52261 gatatgagta aagccagacg ttgggttatc attgtgctat cactggcggt tctggtgatg
    52321 atcggcatta atatggccga aaaagacgat accgcccagg tggtcgtcaa caacaatgat
    52381 cccacctata aaagcgagca tacggacacg ctcgtctata acccagaagg ggcactaagc
    52441 tatcgattga ttgctcaaca cgttgaatat tattccgatc aggccgtttc gtggtttacg
    52501 cagccggtac ttaccacgtt tgataaggat aaaatcccga catggtccgt aaaagcagat
    52561 aaagccaagc tgaccaatga ccggatgctc tatttatatg gacacgttga agtcaacgca
    52621 cttgtgccag actctcaact tcgcagaatc acgacggata acgcgcagat caatctggtg
    52681 acgcaggatg ttacctctga agacctcgtc acgttatacg gaacaacatt taactccagc
    52741 ggtctgaaaa tgcgcggcaa cttacgcagc aagaacgccg agctgattga aaaggttaga
    52801 acatcctatg aaattcaaaa caaacaaact cagccttaat cttgtgcttg ccagctcact
    52861 tctggccgcc agcattccgg catttgccgt aaccggagac actgatcagc cgatccacat
    52921 tgaatcggac cagcaatctc ttgatatgca aggcaacgtg gttaccttta ccggtaatgt
    52981 catcgtcacc cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg
    53041 cggcgaacaa ggtaaagaag tgattgacgg atacggtaaa ccggcaacgt tctaccagat
    53101 gcaggacaac ggtaaacccg ttgaaggtca cgcttcccag atgcactacg aactggcaaa
    53161 agattttgtc gttctgacgg gtaatgctta tctgcagcag gtcgatagca acattaaggg
    53221 cgataagatc acttacctgg tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa
    53281 gcgcgtaacc accgttctgg tgccgtcgca gctgcaggac aaaaacaaca aaggccagac
    53341 cccggcacag aagaagggta attaattcgt tatggcaaca ttaactgcaa agaaccttgc
    53401 aaaagcctat aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca actccgggga
    53461 aattgtcggt ctgctggggc caaacggtgc cggtaagacc accactttct acatggttgt
    53521 aggcattgtg ccgcgcgatg cgggcaacat cattattgat gatgacgata tcagtctgct
    53581 gcctctgcat gcacgcgcgc gccgcggtat cggctatctg ccacaggaag cctccatttt
    53641 ccgtcgcctc agcgtttacg ataacctgat ggcggtactg caaattcgtg acgacttgtc
    53701 tgctgaacaa cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca ttgagcacct
    53761 gcgtgacagc atggggcagt cactctccgg gggtgaacgt cgccgtgtag aaattgcccg
    53821 cgcactggct gcgaatccga aatttattct gctcgacgaa ccgtttgccg gggttgaccc
    53881 gatctcggtt atcgacatta aacgcatcat tgaacacctg cgcgacagcg gcctgggcgt
    53941 gctgatcact gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt
    54001 cagtcagggg catttgatcg cccacggcac gcctacagaa atcttacaag acgaacacgt
    54061 taagcgtgta taccttgggg aagacttcag actctgatag ggtagaagtt tgcgacgttt
    54121 tagcaggaga gtacgattct gaacatgaag caaggtttgc aactcaggct tagccaacaa
    54181 ctggcgatga cgccacagct ccaacaggca attcgtctgt tgcagttgtc gacgctggaa
    54241 cttcagcagg agctacagca ggcgctggag agtaatccgc tgcttgagca aatcgacact
    54301 catgaagaaa tcgacacccg cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg
    54361 ctcgaacaaa aagagatgcc ggaagagctg ccgctcgatg ccagttggga caccatttac
    54421 accgctggta caccatccgg caccagcggt gactacattg acgacgagct gccggtctac
    54481 cagggcgaaa cgacgcagac cttgcaggat tacctgatgt ggcaggtcga gctgacaccg
    54541 ttttccgaca ctgaccgcgc tattgctacc tctatcgtcg atgccgttga tgaaaccggt
    54601 tatctgactg tcccgctgga agatattctc gaaagtatgg gcgatgaaga gatcgacatc
    54661 gacgaggttg aagccgtcct taagcggatc caacggtttg acccggtcgg tgtagcggca
    54721 aaagatctgc gtgactgcct gctaatccaa ctctcccaat tcgataagac cacgccgtgg
    54781 ctggaagagg ccagattgat cattagcgat catctcgatc tgttagccaa tcacgacttc
    54841 cgcactttaa tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg
    54901 atccagtcgc tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc
    54961 attccagatg tgctggtgcg taagcataac ggtcactgga cggtagaact caacagtgac
    55021 agcattccgc gtctgcaaat caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac
    55081 gatggtgaca gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt
    55141 ctggaaagcc gtaacgatac gctactgcgc gtgagtcgct gtatcgttga acagcagcaa
    55201 gccttctttg agcaaggtga agaatatatg aaaccgatgg tactggccga tatcgcccag
    55261 gctgtcgaaa tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata cctgcatagt
    55321 ccacgaggca tttttgaact gaagtatttc ttttccagtc acgtcaatac cgagggcggc
    55381 ggcgaagctt cctccacggc gattcgtgca ctggtgaaga aattaatcgc ggcggaaaac
    55441 ccagcgaaac cgttgagcga cagcaagtta acctctttgc tgtcggaaca aggtatcatg
    55501 gtggcacgcc gcactgttgc gaagtaccga gagtctttat ccattccgcc gtcaaaccag
    55561 cgtaaacagc tcgtttgacc caaccgataa ggaagacact atgcagctca acattaccgg
    55621 aaataacgtc gagatcaccg aggcactgcg cgaatttgtt acagctaaat ttgccaaact
    55681 tgagcaatat tttgaccgaa tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac
    55741 ccacacctca gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg
    55801 tcaggacatg tacgctgcca ttgatggctt aattgataag ctggcacgcc agttgaccaa
    55861 acataaagat aaactgaaac aacactaatt gtccgggcaa ttagcatgtg catggcggcc
    55921 tgttgtgcgg cacaacgggc catttgtacg gttaatgctc cgagcctgtt ccactgtttg
    55981 agtgggcagg ttcttaggtg aaattatgac aaataatgat acaactctac agcttagcag
    56041 tgttcttaac agggaatgta cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct
    56101 ggaaatcatc agcgagctgg cggcgaaaca acttagcctg ccgcctcagg tggtttttga
    56161 agctatcctg acgcgggaaa aaatgggcag taccggtatc ggcaatggta ttgccattcc
    56221 gcatggcaaa cttgaagaag atactctgcg tgccgttggc gttttcgttc agctcgaaac
    56281 gcctatagct ttcgatgcca tcgacaacca accggtggac ttactttttg ccctgctggt
    56341 gccggcagac caaactaaaa cgcacctgca tactctgtcg ctggtggcga aacgtctggc
    56401 ggacaaaacc atctgccgcc gtttacgcgc agcccagagc gatgaagagc tgtatcaaat
    56461 cattacggat accgaaggta ctccggatga agcgtagtta ttcggtaatg tctcttttag
    56521 acgttgtgag gagaaacagt acatggtact gatgatcgtc agcggacgtt caggttcagg
    56581 taaatctgtc gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg ataaccttcc
    56641 cgtagtgttg ttacccgatc tggctcgaac tctggccgat cgcgagattt ctgccgccgt
    56701 cagcattgat gttcgtaata tgccggagtc accagaaata ttcgaacagg cgatgagtaa
    56761 cctgcctgac gctttctcac cgcaactact gttcctggat gccgaccgta ataccttaat
    56821 tcgtcgttac agtgacacgc gccgactgca tccgctttcc agcaaaaacc tgtcgctgga
    56881 aagtgctatc gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag cggatctgat
    56941 tgtcgatacc tcagaaatgt ccgttcacga gctggctgaa atgctgcgta cccgtctgct
    57001 gggtaaacgt gaacgcgaac tgaccatggt ctttgagtct ttcggcttca aacacggtat
    57061 ccctatcgat gcagattacg tctttgacgt gcgcttcttg ccgaacccgc actgggatcc
    57121 gaaactgcgt ccaatgacag gtcttgataa acctgtcgcc gcgttcctcg accgccacac
    57181 agaagtacac aattttatct accagacgcg aagctatctt gagctatggt tacctatgct
    57241 ggaaaccaac aaccgtagct acctgacggt cgccattggt tgtaccggcg ggaagcaccg
    57301 ttcggtgtat attgcagagc aactggcaga ctacttccgc tcgcgcggta aaaacgtcca
    57361 gtcacgccat cgtacgctgg aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc
    57421 acaaacaagc tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt
    57481 tttgacgctg aagtgctgtt acgtaatgac gaaggcaccg aggctgaagc caacagcgtt
    57541 attgcgctgc tgatgttgga ttctgccaaa ggacggcaga ttgaagttga agcgaccggt
    57601 ccacaggaag aggaagcact ggccgccgtt atcgccctct ttaattctgg ttttgatgaa
    57661 gattaatctt catcactttg acatacaact cccttcaagc tcccccgaca ataagaaaat
    57721 cacgtactga aatcgttctc aatcaacgtc atttgtacat tttgtgcgct tttcactttc
    57781 agaagaacct taagaaaacc ttaagaggca ttgtttaggt tttgtttaag ttaatcgacc
    57841 atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa
    57901 gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct
    57961 gtatcaagat tgtctaccat cgtggcgatg gtggcgataa agagatccgc cgtgagttaa
    58021 agtactacgc gcatcttggt cgccggttaa aagactggag tggaataccg cgctatcacg
    58081 gtaccgttga gacggattgc ggaaccggtt atgtctacga tgtgattgcc gattttgacg
    58141 gcaagccttc cattacgctg accgaatttg ctgaacaatg ccgttacgaa gaagacattg
    58201 cacaactgcg ccagctactg aaacagctga agcgttattt gcaggataac cgtatcgtga
    58261 cgatgtcgct gaagccgcag aatatcctct gtcaccgcat cagtgagtca gaggttatcc
    58321 cggtcgtctg cgacaacatt ggtgaaagca cattgattcc actggcgacc tggtcgaagt
    58381 ggtgttgtct gcgtaagcaa gagcggttat ggaaacgatt tatcgcccaa ccggcgctgg
    58441 ctattgccct gcagaaagat ctgcaacctc gtgagagcaa aacgctggcc ctcacttcgc
    58501 gcgaagctta atccagctgg tgttgttgca taaacggctc accgcctaac tgatacatct
    58561 gccgtaaaat ccacgcctga cggctacgca cgtagcccga tggcgaggag actttgaaac
    58621 gaagtggatt aggtaataca gctgccagta atgcagcttc cgaccgggta agtttgctcg
    58681 cgggtttgtg gaaataacgt tgtgccgcag cttcgacgcc aaacacaccg tcgccaaatt
    58741 cggcgatatt caggtaaacc gtcaggatac gctttttgct ccagactgtt tctatcccca
    58801 gcgttaatcc agcttccagc ccttttcgta cccagctacg cccatcccat aaaaagagat
    58861 tttggccgtc tgctgagaaa tcgttgaagc accgcgaata cggttttcat tgcgctcgtt
    58921 gtgcgccagg gctttctcaa tggaagcgac atcaaagccc cagtgctcag gaaatttctg
    58981 atcttctgcg gcaatcaccg ccagtcccat ccacggcgag atttgatcca tactgaccca
    59041 gtcagaatgt gccacgtaac gaaaattgcc atgcagccag gcgctgacct gtcgctcgac
    59101 cattaccgct gagaagggaa caggcgcaac gctaaacaac gcgatgcccc cgccccagaa
    59161 aacggcgagg acaaccatta accgcaaaag aaaacggcga acgaaactaa acaccgttaa
    59221 gcggctttta ctcattcagc cagaaccagc acgcgggaaa ccagcttatc aatgccgctc
    59281 gccgcttctg caatgttctg cgccagcata tatgctgggg tggtgacaat cttattgtct
    59341 tcatcaacca cgatatcatc gacaggacac ggcacatgct ccgcgcccat ctcttccagc
    59401 acttctgcgg tatcgatatc agtaccgatg gtcaaacgca gcgggaaatc gaaaattttc
    59461 ggcagcatcg ccggggcaat acacataaaa ccaagcggtt ttccggcctg atgcatcgct
    59521 tgtgccagcg cctttaattc acggtcaacg gtgcattcgc taccaagact ggcaaaattg
    59581 cttaaattct tcgccgcgcc aaaccccccc ggcacaatca acgcatccag ttcagcggca
    59641 tcggcctggg ccagaggacg gatttcaccg cgcgttattc gtgccgcttc aatcagcaca
    59701 ttgcgcgttt ccgtcatcgc ttcgccagtt aaatggttga taacatcaac ctgctgctta
    59761 tccggtgcaa agcagaccgc ctgcgcaccg ctgcgtgaaa tagctaacaa cgtcaacacc
    59821 gcttcatgaa tttcagaacc gtcatagacg ccgcatccgc tcagaattac gccaattttc
    59881 ttcattgtga tcatcctttc gcaattgact gaaacacatt aattttttta ataaaaatgg
    59941 tacgcatcac acatttaact gattcatgta acaaatcatt taagttttgc tatcttaact
    60001 gcgtgcggcc tgaaaaacag tgctgtgccc ttgtaactca tcataataat ttacggcgca
    60061 gccaagattt ccctggtgtt ggcgcagtat tcgcgcaccc cggtctagcc ggggtcattt
    60121 tttagtggct tttgccaccc acgctttcag cacttctacg tcgtgacgcc actcttcttt
    60181 catctcttca atccattcac cgacgttatc ttcccaggcg ggaaggtcag gagactgaat
    60241 ttgctgaccc agctgttgca gatggcgtaa ccccactgac cccgccgcac ctttaatttt
    60301 atgtccttcc tcaacaatgc cttttttatc ctgcgccgtc agattcgact ccagcacgct
    60361 gacatagccc ggcatcatct tctcaaacac cgctaacccg tcggtgatca gcttcggtcc
    60421 tacaagttcg agatactgtt ccagcatggg aatatcgagc aatgcttctg atttactgtt
    60481 ctcttctgtc gtcaccgtac tctcctcatc atcctgggta tcccagaatt tcttgatcat
    60541 cgcggttagc gccggaaccg aaagcggctt actcagcaca tcatccattc cagcattgag
    60601 gtactcttgt ttgtctttca gcacgttagc ggttaaggcc accagcggcg gtaaatcctc
    60661 gcgcggataa cgcttcgtca gttcacgaga gatatccagc ccggtcatat ctggcaactg
    60721 aatatccagc aacaccaggt cgtattcgcc cggtttaaac atctccagcg ccgccttgcc
    60781 ggtcatggcg acatcaacgc tgttacctaa tttttccagc acagaacgcg cgacaatcac
    60841 gttcagttca atgtcttcca ccagcagcac attcagcgcc ggtaaaggca tatcgtcttc
    60901 atcaaacgca tcatcgacct cttctgccac cgacggtgcg tggatcgtca acgtaaaggt
    60961 tgaacctttg ccctgttcac tggtaaccgt aatatcgccg cccatatttt tcgccagacg
    61021 acgagaaacg gccagaccaa taccggtgcc ggtggcaggt ttaccgccat gactgtcttt
    61081 cacctggtaa tacatggcga agattttatc cagctcatcc tgcggaatgc cgataccaga
    61141 atcttccact tcaaaatgca gcatatcgcc ttcatcgtag cgcacgcgca cggtaacctg
    61201 gccttgctgg gtgaatttga cggcgttact gatgaggttc cacaggatct gccgtaaacg
    61261 cgtcccgtcg gtaatgacct gatgcggtaa tggcagcgta ggctccaggt taaagcgcaa
    61321 tcctttttgt tgcgcctgca atgcggagag attttccaga tcggcaagga agctggtgaa
    61381 atcaaccggc tgattatcaa gctggacctt gcgccgttcc atcttatcca tgtcgataat
    61441 atcgttaaag atattcccca gcgtgacggc cgaaacatgg atagttttga gatatttttc
    61501 ctgctcggcg gtgagttcgg tatccagcag aatgcggctc aggccgacga taccgttcag
    61561 cggtgtacgc aattcgtgac tgatggtgga aataaacgtc gttttgtcgc ggctggcgcg
    61621 ttcaagcgca tcctgatacc gcttacgctc ggtaatgtcg cgaccaaagc ccatcaaacc
    61681 gtgacgttta cccacgcggt cgtagtacgg cactttacgg atttcaaagc aggctttgcg
    61741 cccgtccggg taatccagcc actgttcata ggtcagtgac acattatgac ggaacacttt
    61801 ttcatcggtt tcaatgactt ttgcggcggc ttccggtgag taaacatcag caggtttcag
    61861 gtgaaccagt tgtttttcgc tttttccggt cagcagctcc atcgcgcggt tacagccgga
    61921 aaactcttta tcttcgttac gataaaaaac caagtcgggt gaagcatcaa ggaaggaacg
    61981 taagaatgag gattgctgct cgagctgaat ttgtgtctct tcgcgctctt tgatttcaat
    62041 tttcagttgg ccgaaggttt cctgcagttc tgcttccgct ttttcacgaa cggcaatttc
    62101 ctgatttagc tgggcaatat tatcttttaa ctgaacgttg aggctcaaat cgcgctcgcg
    62161 catctcctcc agtttttgca ccagccgtga cagacgttgt cgtgactcct ccagttgctc
    62221 gacgaccacc gatagaaagt agaccgccca cggcgtaatc agcaaaccaa agaagataga
    62281 acgaataaca tcaatgcttt cgacctgacc atgcagcacc atggttaccg ccatttgtac
    62341 cacaatggca agaacgacga gggccagcgc cagcaacatt gagaagcgca ccagacctaa
    62401 cttcatcatc aggtcaacat aatactgcgc cagcagacga atttgcttca ttagggaatt
    62461 ccttcacgac aacctgcccc aataataccc aattctgaca gttacgttat aaattgtgtg
    62521 agaaatgcgg aattcccctc aacgacctac tccgttggag gtagccacgg acgtcccagc
    62581 gccgatccct gtacgccatg ttcattcaga tagcgatcca gctcgaccat tcccgtccat
    62641 cgattttcgc accacagcgg agcaagcagc gtcgggcgac gggcgctggc ggaaatgcgg
    62701 tggtagatca cttccggtgg cgtatggcga atcatttctc cggcagtgag cgtataatcc
    62761 tccagttcaa taccgttcaa acgccccgct tcccaggctt tcgccataat gctgcctttc
    62821 acaatatgca gcggatgcag ctttatgccg tccacgctgg tttcaaccac tcgctccagc
    62881 gtttgcaaac attcagcctg cccttcgcca ggcaggccga caattaagtg ggaacacacc
    62941 ttcagcccgc gctgacgtgc cagctgggtt gtacgctgat aacaggcaaa atcatggccg
    63001 cggttgatgc gatgcagtgt tttgtcgtgg gcggtttgta gccccagctc cagccacact
    63061 tcgtaaccct ggtccttata ttcgcaaagc agatccagca ccgcatccgg cacgcagtcc
    63121 gggcgggtac caacacacaa accgacaata ttggcctggc tcaccgcctg ctgatacata
    63181 gaacgcagca cctgaacttc cgcaaaggtg ctggtatacg cctgaaagta ggccagatag
    63241 cgtttagcgc ggttaactaa attcgcctgg tgcgccagtt gctcggcaat ggaacgatgc
    63301 tgctgcgctt catcggcaaa cgaggcaaca ttacagaatg tgcagccacc acgcccgatg
    63361 gtaccgtcac ggttagggca gctaaaaccg ccgtgcagcg tcagcttatg caccttttgc
    63421 ccataacgac gggtgagatc accaccaaac atattgacta atttctgtaa ctgcataatc
    63481 tgatagaccg cgccttgaaa agaggccaaa gcctgccatt tttagctcaa ttcggcgatg
    63541 acctggatca atcgtcccgg cctgctttta tcaactgcat aatcaatcaa aattaccgaa
    63601 atttcatgca caatcacata aatcactttt gcttatcttg tgtcagattt ttttatctcc
    63661 ttatggattt taggcaaaaa cagtagcatg aaacgtcatt accaattaag gcagtataaa
    63721 atgctggttt tctcgtcagt tcaaggcagg ataagggtta acacgccttt atgacagtca
    63781 ggaattgact gtttctctaa cgactctcct tttagcctta aagataaaat ctattttaat
    63841 ttcagctatt taataaagaa ttttgcgcta aagcacattt ctgtaccaat aagcttgcca
    63901 tttgacctgt atcagctttc ccgataagtt ggaaatccgc tggaagcttt ctggatgagc
    63961 agcctgctca tcatatttat gcagtaattg agatcccctc ttcaccgtat taaccgatgc
    64021 gaaaaggaca acaagggggc gaatgcgagg cgcgcgtatg acacgcaaac cccgtcgcca
    64081 cgctctttct gtgcccgtgc gcagcggttc ggaagtgggg ttcccgcaga gcctggggga
    64141 ggttcacgat atgttgtacg ataaatccct tgagagggat aactgtggtt tcggcctgat
    64201 cgcccacata gaaggcgaac ctagccacaa ggtagtgcgt actgcaatac acgcactggc
    64261 ccgcatgcag caccgtggcg cgattctcgc cgatggtaaa accggcgacg gttgcggctt
    64321 gctgttacaa aaaccggatc gcttttttcg catcgttgcg caggagcgcg gctggcgttt
    64381 agcaaaaaac tacgctgtcg ggatgctctt cctgaataaa gatcctgaac tcgccgctgc
    64441 cgcacgccgc atcgttgaag aagaactgca acgcgaaacc ttgtcgattg tgggctggcg
    64501 tgatgtcccc actaacgaag gcgtgctggg tgaaatcgcc ctctcctctc tgccacgcat
    64561 tgagcaaatt tttgtgaacg ccccggcagg ctggcgtcca cgcgatatgg agcgccgtct
    64621 gtttatcgcc cgccgccgca ttgaaaagcg tctcgaagcc gacaaagact tctacgtctg
    64681 tagcctgtcg aatctggtga acatctataa aggtctgtgt atgccggcgg atctgccgcg
    64741 cttttatctg gatcttgcgg acctgcgtct ggaatcggcc atttgcctgt tccaccagcg
    64801 cttctccact aacaccgtac cgcgctggcc gctggcgcaa ccgttccgct atctggcgca
    64861 taacggtgaa atcaacacca tcaccggtaa ccgccaatgg gcgcgtgcgc gtacctataa
    64921 attccagacg ccgctgatcc ctgacctgca cgacgccgcg ccattcgtca acgaaaccgg
    64981 ttctgactcc agttccatgg ataacatgct ggaactgctg ctggcaggcg ggatggatat
    65041 catccgcgcc atgcgcctgt tagtgccacc cgcctggcag aacaacccgg atatggaccc
    65101 ggaactgcgt gccttctttg actttaactc catgcatatg gagccgtggg atggtccggc
    65161 gggcatcgtg atgtccgatg gtcgcttcgc tgcctgtaac ctcgaccgta acggtctgcg
    65221 tccggcacgc tacgtcatta ccaaagacaa gctcatcacc tgcgcctctg aagtcggtat
    65281 ctgggattac cagcctgacg aagtggtcga aaaaggccgc gtcgggccag gcgaactgat
    65341 ggtgatcgac actcgcagtg ggcgtattct gcactcggca gaaaccgatg acgatctgaa
    65401 aagccgccac ccttataaag agtggatgga gaaaaacgtc cgccgactgg taccgtttga
    65461 agatctgccc gatgaagaag tgggtagccg cgaactggac gacgacacgc ttgccagcta
    65521 ccagaaacag tttaactaca gcgcggaaga gctggactcc gtaattcgcg tactgggcga
    65581 aaacggtcag gaagcggtcg gttcgatggg cgatgatacc ccattcgccg tgctctccag
    65641 tcagccgcgc attatttacg actacttccg ccagcagttt gcccaggtga ctaacccgcc
    65701 aatcgacccg ctgcgtgaag cgcatgttat gtcgctcgcc accagtatcg gtcgtgaaat
    65761 gaacgtcttt tgcgaagcag agggccaggc gcaccgttta agctttaaat cgccgattct
    65821 gctctactcc gatttcaaac agctcacgac gatgaaagag gagcactacc gcgcagatac
    65881 gctggatatc acctttgacg tcactaaaac cacgctcgaa gcgacagtca aagagctatg
    65941 cgacaaagcc gaaaaaatgg tacgtagcgg caccgtgttg ctggtgctct ccgaccggaa
    66001 tatcgctaaa gatcgcctgc cggttccggc tccgatggcg gttggcgcga tccagacccg
    66061 tctggtcgat caaagcctgc gttgcgatgc caacatcatc gtcgaaaccg ccagcgcccg
    66121 cgatccgcac cacttcgccg tgttgctggg tttcggcgcg acggctattt atccgtacct
    66181 tgcctatgaa acgctgggcc gcctggtaga cacccacgcg attgccaaag attatcgtac
    66241 cgtgatgctc aactaccgta acggcatcaa caaaggcttg tacaaaatca tgtccaaaat
    66301 gggcatctcc accatcgcct cttaccgctg ctcgaaactg tttgaagcgg tcggtctaca
    66361 cgatgatgta gtgggcctgt gcttccaggg cgcggtcagc cgcattggtg gggcaagctt
    66421 tgaagacttc cagcaggatc tgctgaatct gtcgaaacgt gcctggctgg cgcgcaagcc
    66481 gattagccag ggcggcctgc tgaaatacgt ccacggcggc gaataccacg cctacaaccc
    66541 ggacgtggtg cgcacgctgc aacaggcggt acaaagcggc gagtacagcg actatcagga
    66601 atacgcgaag ctggttaatg agcgtccggc aaccacgctg cgcgatctgc tggcaattac
    66661 gccgggtgaa aacgcggtca acattgctga tgttgaaccg gcaagcgaac tgtttaaacg
    66721 ctttgatacc gccgcgatgt ctatcggcgc gttaagcccg gaagcccacg aggcgctggc
    66781 ggaagcgatg aatagcatcg gcggtaactc gaactccggt gaaggcggcg aagacccggc
    66841 gcgctatggc actaacaaag tgtcgcgcat caagcaggtg gcttctggtc gcttcggcgt
    66901 tactccggcg tatctggtca atgccgacgt cattcagatt aaagtcgccc agggcgcgaa
    66961 gccgggcgaa ggtggtcagt tgccgggcga caaagtcact ccttacatcg ccaaactgcg
    67021 ctattcggtg cccggagtga cgctgatctc cccgccgccg caccacgata tctactctat
    67081 cgaagactta gcgcagctta ttttcgacct caagcaggtt aacccgaaag cgatgatctc
    67141 cgttaagctg gtgtccgaac caggcgttgg caccatcgcg actggcgtgg cgaaagctta
    67201 tgcggacttg atcaccatcg cgggctatga cggcggcacc ggcgcaagcc cgctgtcgtc
    67261 agtgaaatat gcaggctgcc cgtgggaact cggtctggtg gaaacccaac aggcgctggt
    67321 tgccaacggc ttgcgtcata aaattcgttt gcaggttgat ggcggcctga aaacgggtgt
    67381 cgatatcatc aaagcggcga ttctcggcgc agaaagcttc ggcttcggca ctggcccaat
    67441 ggtggcgctc ggctgtaaat atctgcgtat ttgccacctg aacaactgcg ccacgggtgt
    67501 agcaactcag gatgacaaac tgcgtaagaa tcactatcac ggcctgccgt tcaaggtgac
    67561 taattacttt gagtttatcg cccgtgaaac ccgcgagctg atggcacagc tgggagtaac
    67621 gcgtctggtg gatctgattg gtcgcaccga cctgctgaaa gagctggacg gtttcaccgc
    67681 caaacagcag aaactggcgc tgtcgaagct gctggagact gccgagccac atgcaggtaa
    67741 gtcactctac tgcactgaaa acaacccgcc gtttgataac ggcctactga acgcgcagtt
    67801 gctgcaacag gcgaaaccgt ttgtcgatga acgccagagc aaaaccttct ggttcgatat
    67861 ccgcaacacc gaccgttctg tcggcgcgtc gctttcaggc tatatcgccc agacgcacgg
    67921 cgatcagggg ctggcagccg atcctatcaa agcgtacttc aacggcaccg caggccagag
    67981 tttcggtgtg tggaacgcgg gcggcgtgga actgtacctg accggggatg ccaacgacta
    68041 tgtcggtaaa ggcatggcgg gcggcttaat cgccattcgt cctccggttg gttccgcctt
    68101 ccgcagccat gaagcaagca ttatcggcaa cacctgcctg tatggcgcga ccggtggtcg
    68161 tctgtatgcc gcaggccgcg cgggtgaacg tttcggcgtg cggaactccg gtgctatcac
    68221 cgtggtagaa ggcattggcg acaacggctg tgaatatatg acgggcggta ttgtctgtat
    68281 tctgggtaaa accggcgtta acttcggcgc gggcatgacc ggcggcttcg cttacgttct
    68341 cgatgaaagc ggtgatttcc gcaaacgcgt taacccggaa ctggtcgaag tcttaagcgt
    68401 cgacgatctg gcgatccatg aagagcatct gcgcggtctt atcaccgagc atgttcagca
    68461 taccggttct cagcgcggtg aagagattct ggcgaactgg tcaaccttcg ccacgaaatt
    68521 cgcgctggtt aaaccgaagt ccagtgatgt aaaagcactg ctgggtcacc gtagtcgtag
    68581 cgcagcagag ttgcgcgtgc aggcgcagta aggggtagca acaatgagtc agaatgttta
    68641 tcaatttatc gacctgcagc gcgttgatcc gccaaagaaa ccgctgaaga tccgtaaaat
    68701 tgagtttgtt gaaatttacg agccgttttc cgaaggccag gccaaagcgc aggctgaccg
    68761 ctgtctgtcg tgcggcaacc catactgcga gtggaaatgc ccggtacaca actacatccc
    68821 aaactggctg aagctcgcca acgaggggcg tatttttgaa gcggcagaac tgtcgcatca
    68881 gaccaacaca ctgccggaag tatgcggacg tgtctgcccg caagaccgtc tgtgcgaagg
    68941 ttcctgcacc ctgaacgatg agttcggtgc ggtgaccatc ggcaacattg agcgttatat
    69001 caatgataaa gcgttcgaaa tgggctggcg tccggatatg tccggcgtga agcaaaccgg
    69061 taaaaaagtg gcgattatcg gtgcaggccc ggcaggtctg gcgtgtgcgg atgtcctgac
    69121 gcgtaacggc gtaaaagccg ttgtcttcga ccgtcatcca gaaattggcg ggctgctgac
    69181 cttcggtatt ccggccttca agctggaaaa agaggtaatg acgcgtcgcc gtgaaatctt
    69241 caccggcatg ggtattgaat tcaaactcaa taccgaagtg ggccgcgacg tacagctgga
    69301 cgatctgctg agtgattacg atgccgtgtt ccttggcgtt gggacttatc agtcaatgcg
    69361 cggcgggctg gaaaacgaag acgccgatgg cgtgtacgca gcgctgccgt tccttatcgc
    69421 caacaccaaa cagttaatgg gctttggtga aacccgcgac gaaccgttcg tcagcatgga
    69481 aggcaaacgc gtggtagtac ttggcggtgg cgacactgcg atggactgcg tgcgcacgtc
    69541 tgtacgccag ggggcaaagc acgttacctg tgcctatcgt cgtgatgaag agaacatgcc
    69601 gggttcccgc cgcgaagtga aaaacgcgcg ggaagaaggc gtagagttca aattcaacgt
    69661 ccagccactg gggattgaag tgaacggtaa cggcaaagtc agcggcgtaa aaatggtacg
    69721 taccgaaatg ggcgaaccgg atgccaaagg ccgtcgccgc gcggagatcg ttgcaggttc
    69781 cgaacatatc gttccggcag atgcggtgat catggcgttt ggtttccgtc cacacaacat
    69841 ggaatggctg gcaaaacaca gcgtcgagct ggattcacaa ggccgcatca tcgctccgga
    69901 aggcaacgac aacgctttcc agaccagcaa cccgaaaatc tttgctggcg gcgatatcgt
    69961 ccgtggttcc gatctggtcg ttaccgccat cgccgaaggt cgcaaagcgg cagacggcat
    70021 tatgaactgg ctggaagttt aagctctgtc ccagcgccgc ctcctcagaa aagagtcggc
    70081 gctgaaattc tcctttcttt ctggaaatcg cctcgcataa tctgaagaaa accaatgtaa
    70141 tacgctcatc tggattgtta ccggtagatg cccggcgtat agctgattcc attattcaac
    70201 gcagcgccat ataaagggac agataatgga atcgctctca gagagaacct caacaggcta
    70261 ccagcaaatc cacaacggca ttattcatct ggtcgatagc gcccgaacgg aaacggtacg
    70321 tagcgttaac gcgctgatga ccgcgacgta ctgggaaatt ggccgacgga ttgtcgaatt
    70381 tgaacaaggt ggtgaggcca gagcagctta tggtgcacag ttgattgatc ggctatcgaa
    70441 ggatttaagt cgacggtata aacgtggggt ctctgcaaaa aacttacgcc aaatgaggct
    70501 tttttacctc ttttttcaac atgttgaaat tcgccagaca gtgtctggcg aattaacacc
    70561 attgggaatt ccgcagacac catctgcaga attcccatcg gcaaagattt ggcagacgct
    70621 gtctgccaaa ttgagaggct gtttgatatt tccacagctt ttgtattgca tctcctattt
    70681 tcggtttgta tattcatgca aataaacgaa gg
//
LOCUS       DAZPAB010000003        60409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZPAB010000003 DAZPAB010000000
VERSION     DAZPAB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.003"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..322
                     /locus_tag="ACHUPG_000157"
     CDS             <1..322
                     /locus_tag="ACHUPG_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0859933.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="ACHUPG_000158"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="ACHUPG_000158"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR0859934.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="ACHUPG_000159"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="ACHUPG_000159"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR0859935.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="ACHUPG_000160"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="ACHUPG_000160"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR0859936.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="ACHUPG_000161"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="ACHUPG_000161"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR0859937.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="ACHUPG_000162"
     CDS             complement(3726..4955)
                     /locus_tag="ACHUPG_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR0859938.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="ACHUPG_000163"
     CDS             complement(5019..5459)
                     /locus_tag="ACHUPG_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR0859939.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="ACHUPG_000164"
     CDS             complement(5564..6334)
                     /locus_tag="ACHUPG_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0859940.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="ACHUPG_000165"
     CDS             complement(6331..7257)
                     /locus_tag="ACHUPG_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0859941.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="ACHUPG_000166"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="ACHUPG_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR0859942.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="ACHUPG_000167"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="ACHUPG_000167"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR0859943.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /gene="gcd"
                     /locus_tag="ACHUPG_000168"
     CDS             8811..11201
                     /gene="gcd"
                     /locus_tag="ACHUPG_000168"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR0859944.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="ACHUPG_000169"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="ACHUPG_000169"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR0859945.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMVIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="ACHUPG_000170"
     CDS             12964..13311
                     /locus_tag="ACHUPG_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR0859946.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="ACHUPG_000171"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="ACHUPG_000171"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR0859947.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="ACHUPG_000172"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="ACHUPG_000172"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR0859948.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /gene="yacL"
                     /locus_tag="ACHUPG_000173"
     CDS             complement(15131..15493)
                     /gene="yacL"
                     /locus_tag="ACHUPG_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR0859949.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="ACHUPG_000174"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="ACHUPG_000174"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR0859950.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18620..20473
                     /locus_tag="ACHUPG_000175"
                     /pseudo
     CDS             18620..20473
                     /locus_tag="ACHUPG_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="ACHUPG_000176"
     CDS             complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="ACHUPG_000176"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR0859951.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="ACHUPG_000177"
     CDS             complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="ACHUPG_000177"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR0859952.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="ACHUPG_000178"
     CDS             complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="ACHUPG_000178"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR0859953.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="ACHUPG_000179"
     CDS             complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="ACHUPG_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR0859954.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28317..29690
                     /gene="aroP"
                     /locus_tag="ACHUPG_000180"
     CDS             28317..29690
                     /gene="aroP"
                     /locus_tag="ACHUPG_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR0859955.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="ACHUPG_000181"
     CDS             complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="ACHUPG_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR0859956.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="ACHUPG_000182"
     CDS             complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="ACHUPG_000182"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR0859957.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31223..32116
                     /gene="nadC"
                     /locus_tag="ACHUPG_000183"
     CDS             31223..32116
                     /gene="nadC"
                     /locus_tag="ACHUPG_000183"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR0859958.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32319..32759
                     /gene="ppdD"
                     /locus_tag="ACHUPG_000184"
     CDS             32319..32759
                     /gene="ppdD"
                     /locus_tag="ACHUPG_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR0859959.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32769..33916
                     /locus_tag="ACHUPG_000185"
                     /pseudo
     CDS             32769..33916
                     /locus_tag="ACHUPG_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
     gene            33906..35108
                     /gene="hofC"
                     /locus_tag="ACHUPG_000186"
     CDS             33906..35108
                     /gene="hofC"
                     /locus_tag="ACHUPG_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFR0859960.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="ACHUPG_000187"
     CDS             complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="ACHUPG_000187"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR0859961.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36348..36386)
                     /locus_tag="ACHUPG_000188"
                     /pseudo
     CDS             complement(36348..36386)
                     /locus_tag="ACHUPG_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            36411..37031
                     /gene="coaE"
                     /locus_tag="ACHUPG_000189"
     CDS             36411..37031
                     /gene="coaE"
                     /locus_tag="ACHUPG_000189"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR0859962.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37031..37774
                     /gene="zapD"
                     /locus_tag="ACHUPG_000190"
     CDS             37031..37774
                     /gene="zapD"
                     /locus_tag="ACHUPG_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR0859963.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37784..37981
                     /gene="yacG"
                     /locus_tag="ACHUPG_000191"
     CDS             37784..37981
                     /gene="yacG"
                     /locus_tag="ACHUPG_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR0859964.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="ACHUPG_000192"
     CDS             complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="ACHUPG_000192"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR0859965.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38530..41235)
                     /gene="secA"
                     /locus_tag="ACHUPG_000193"
     CDS             complement(38530..41235)
                     /gene="secA"
                     /locus_tag="ACHUPG_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR0859966.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41297..41809)
                     /gene="secM"
                     /locus_tag="ACHUPG_000194"
     CDS             complement(41297..41809)
                     /gene="secM"
                     /locus_tag="ACHUPG_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR0859967.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNPNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="ACHUPG_000195"
     CDS             complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="ACHUPG_000195"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR0859968.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="ACHUPG_000196"
     CDS             complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="ACHUPG_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR0859969.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="ACHUPG_000197"
     CDS             complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="ACHUPG_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR0859970.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="ACHUPG_000198"
     CDS             complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="ACHUPG_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR0859971.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="ACHUPG_000199"
     CDS             complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="ACHUPG_000199"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR0859972.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47274..48749)
                     /gene="murC"
                     /locus_tag="ACHUPG_000200"
     CDS             complement(47274..48749)
                     /gene="murC"
                     /locus_tag="ACHUPG_000200"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR0859973.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48803..49870)
                     /gene="murG"
                     /locus_tag="ACHUPG_000201"
     CDS             complement(48803..49870)
                     /gene="murG"
                     /locus_tag="ACHUPG_000201"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR0859974.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="ACHUPG_000202"
     CDS             complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="ACHUPG_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR0859975.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51111..52427)
                     /gene="murD"
                     /locus_tag="ACHUPG_000203"
     CDS             complement(51111..52427)
                     /gene="murD"
                     /locus_tag="ACHUPG_000203"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR0859976.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="ACHUPG_000204"
     CDS             complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="ACHUPG_000204"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR0859977.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53506..54864)
                     /gene="murF"
                     /locus_tag="ACHUPG_000205"
     CDS             complement(53506..54864)
                     /gene="murF"
                     /locus_tag="ACHUPG_000205"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR0859978.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54861..56348)
                     /gene="murE"
                     /locus_tag="ACHUPG_000206"
     CDS             complement(54861..56348)
                     /gene="murE"
                     /locus_tag="ACHUPG_000206"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR0859979.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="ACHUPG_000207"
     CDS             complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="ACHUPG_000207"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR0859980.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="ACHUPG_000208"
     CDS             complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="ACHUPG_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR0859981.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="ACHUPG_000209"
     CDS             complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="ACHUPG_000209"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR0859982.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="ACHUPG_000210"
     CDS             complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="ACHUPG_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR0859983.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14470 a        16732 c        14845 g        14362 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctaccca aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg tccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccagg gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcacaagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgcacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagtg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 caccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgttgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
    18721 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
    18781 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
    18841 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
    18901 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    18961 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    19021 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    19081 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    19141 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
    19201 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    19261 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
    19321 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    19381 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    19441 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
    19501 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
    19561 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
    19621 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    19681 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    19741 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
    19801 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    19861 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    19921 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    19981 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
    20041 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
    20101 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
    20161 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
    20221 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    20281 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
    20341 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
    20401 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
    20461 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    20521 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
    20581 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    20641 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
    20701 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
    20761 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
    20821 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    20881 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    20941 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    21001 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    21061 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    21121 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    21181 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    21241 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    21301 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    21361 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    21421 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
    21481 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
    21541 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
    21601 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    21661 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
    21721 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    21781 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    21841 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    21901 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    21961 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
    22021 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
    22081 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
    22141 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
    22201 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
    22261 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
    22321 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
    22381 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
    22441 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
    22501 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
    22561 tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac
    22621 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
    22681 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
    22741 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
    22801 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
    22861 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
    22921 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
    22981 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
    23041 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
    23101 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
    23161 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
    23221 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
    23281 gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg
    23341 aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc
    23401 acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc
    23461 agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact
    23521 tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc
    23581 gctgccggag tcgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact
    23641 tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct
    23701 ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg
    23761 atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca
    23821 acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct
    23881 gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg
    23941 tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact
    24001 ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct
    24061 tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg
    24121 gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt
    24181 ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg
    24241 cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa
    24301 ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac
    24361 gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct
    24421 gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct
    24481 gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat
    24541 cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc
    24601 cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc
    24661 ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac
    24721 ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag
    24781 tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca
    24841 taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca
    24901 gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac
    24961 gaccggaagt accgccgatc aggaagccac gcgcttgctg gtctccagcc gcccagcaca
    25021 gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca
    25081 gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt
    25141 tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc
    25201 ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac
    25261 cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg
    25321 acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagagctctt
    25381 tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg
    25441 tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat
    25501 aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat
    25561 cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct
    25621 taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg
    25681 tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt
    25741 agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt
    25801 ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac
    25861 catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca
    25921 gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga
    25981 tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac
    26041 cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca
    26101 gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt
    26161 cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat
    26221 atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg
    26281 ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt
    26341 gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac
    26401 gggcgtaaac gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct
    26461 cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga
    26521 aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga
    26581 tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact
    26641 ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta
    26701 cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct
    26761 caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg
    26821 gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt
    26881 gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta
    26941 cgtttagtta ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac
    27001 cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc
    27061 tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct
    27121 tcgcgcgctt cttccggctt accgaccata atcgcttcaa atatgcgggt gcggtgacta
    27181 ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc
    27241 tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct
    27301 tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac
    27361 tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta
    27421 ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag
    27481 tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc
    27541 cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct
    27601 tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt
    27661 tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc
    27721 agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt
    27781 tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct
    27841 aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg
    27901 cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga
    27961 ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg
    28021 attcacttac caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca
    28081 tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca
    28141 accatgcata aagcgggtgc attcgctgcc gcataccatt attcttgatc tgacggaagt
    28201 ctttttgtaa caattcaaac ctccttgatg taaacaaatc aatacaacaa acggaattgc
    28261 aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga
    28321 tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc
    28381 ttatcgcgct gggtggcgcg atagggacag ggctattcct aggtagcgcc tccgtaatac
    28441 agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga
    28501 tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact
    28561 ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac
    28621 tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt
    28681 atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca
    28741 acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta
    28801 ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggcggcc
    28861 cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg
    28921 gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga
    28981 tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta
    29041 tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt
    29101 ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata
    29161 cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca
    29221 gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa
    29281 aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc
    29341 tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt
    29401 taatggcact ggtggtatcc gcactggtga tcaactgggc gatgatcagt ctggcgcata
    29461 tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt
    29521 atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga
    29581 tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta
    29641 tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg
    29701 ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt
    29761 agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc
    29821 ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt
    29881 aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct
    29941 ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc
    30001 agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc
    30061 gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag
    30121 gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc
    30181 aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga
    30241 atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct
    30301 gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc
    30361 agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt
    30421 aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt
    30481 ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta
    30541 aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc
    30601 tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg
    30661 gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg
    30721 cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc
    30781 cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga
    30841 ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc
    30901 gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct
    30961 gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg
    31021 gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt
    31081 aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg
    31141 atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc
    31201 gttagctatc tggagtttta acatgccgcc tcgccgctac aaccctgaca cccgacgtga
    31261 cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga
    31321 agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taccggaaaa
    31381 ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg
    31441 ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga
    31501 tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt
    31561 gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag
    31621 taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg
    31681 caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc
    31741 gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgtctc
    31801 cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga
    31861 agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat
    31921 catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa
    31981 ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac
    32041 gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc
    32101 aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc
    32161 cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa
    32221 aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt
    32281 tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta
    32341 cacttatcga actgatggtg gttattggca tcattgccat tttaagctcc attggtattc
    32401 ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc
    32461 cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg
    32521 gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg
    32581 caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga
    32641 cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa
    32701 gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag
    32761 gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc
    32821 tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc
    32881 tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac
    32941 aaatggaagg tcacgccagt cgcacacaac agacattacc cgtagctgtt caggagaagc
    33001 atcagcccaa agcagagttg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt
    33061 ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg
    33121 tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa
    33181 aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg
    33241 tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg
    33301 aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg
    33361 gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg
    33421 tgctggtaac tggccctaca ggcagtggca aaacggtcac ctggtgttgt ccacgctaca
    33481 caccaattcc accactgaaa cgctggtacg tttacagcaa atgggggtcg cccgctggat
    33541 gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca
    33601 ttgtcgccga cagcaagggg agcccatcca tattccagac actgtatggc cgtcgccgct
    33661 gccccactgg caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtaccgc
    33721 cttgtttgaa gtcctgccca ttacaccagc catacgccag cttatttccg ctaatactga
    33781 agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg cgtacgcttt ttgaaaacgg
    33841 ctgcctggcc gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat
    33901 gccgcatggc aagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc
    33961 aagatgggat gctatgggca gagagccgta ctttactgct tatggcacta cagcaacaga
    34021 tggttacccc actaagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata
    34081 aaagcgcgga agtcattcat caactggcga cgctacttaa agccgggtta acgctttctg
    34141 aagggctggc tctgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat
    34201 cgctggcgca cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag
    34261 aggcatttcc gccgctctat caggcgatga tccgcacggg tgaactgacc ggtaagctgg
    34321 atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca gcgtcagttg accgacaaag
    34381 tgaaatcagc gttgcgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg
    34441 caatgctgca ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac
    34501 taccggcact aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggggctggc
    34561 tgctggtgtt gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgcccga
    34621 cctggcttat cgcgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatga
    34681 ggggacaaaa actcacgcag atctttacga ttctggcgct gacacaaagt gcaggcatta
    34741 cttttttaca gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac
    34801 ttctgacaca aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa
    34861 atgccggtga gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct
    34921 cgctggacct catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg
    34981 cggataacct cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg
    35041 gtacgctggt ggtggcaatg tatctgccaa ttttccattt aggcgatgcg atgagtggga
    35101 tgggataacg ctggcgtgtt gctccacgcc agcgttggga gattacaggt tgttgaagat
    35161 gcggttttct tgttccagca cacgaataaa cgtggtgcgc ttggtcagct ctttcaggcg
    35221 tgaagcccca acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt
    35281 ttcaacctgg cctcgcagcg gcagcttaac ggttttacct tctgctgcgc ggtattccgc
    35341 aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa
    35401 tttctcgccg ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc
    35461 gccaagcatg acgaaatcgg caccgccgcc aaaggctttc gccacatcgc ccggcgtggt
    35521 acagccaccg tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat
    35581 taccgcagaa agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc
    35641 tgggccaatg ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc
    35701 accagtcact acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc
    35761 aacgaactgc acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa
    35821 tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtgccggt
    35881 agaaaccatc acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc
    35941 ttcgacagaa aagtgtttat gcacagcagt cagaatatca aaagaagcca gcgcagaggc
    36001 catagaaaat gtgcctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct
    36061 ctgacctgaa tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag
    36121 agtggagcgt ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat
    36181 acgcatgtgc ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg
    36241 ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca
    36301 atcccataaa tttacctggt aaatagtgag cgaacgtttc atcaatttca ctttcgcgcg
    36361 ctcattttta acgttttgat aatcatgatg aattcccggg aatataaatt atggggtata
    36421 tagttgcctt aacgggaggc attggcagtg gtaagagtac cgttgccaat gcgtttgctg
    36481 atctcggaat taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg
    36541 cacctgcgct acatgccatt gctgatcact ttggcgaaaa catgattgct gctgatggaa
    36601 cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc
    36661 ttaacgacct gctgcatccg ctgattcaac aagagacgca acaccagatc cagcaagcaa
    36721 cttcccccta tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag
    36781 cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc
    36841 gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc tgctcaggca acgcgcgaag
    36901 cccgccttgc cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat
    36961 cggatgttgc ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg
    37021 aaaaaccgta atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac
    37081 atggctgcgc attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca
    37141 cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg
    37201 cgaagtccgc actgagctgt tgaaagaact taaccggcag caacgtaaac tccagacctg
    37261 gattggcgtg cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc
    37321 ggcggtgagc gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt
    37381 gattgctctg gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc
    37441 tacattgcac atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg
    37501 gattgccagc ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca
    37561 gtcggccccc ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga
    37621 tgccgacttg ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg
    37681 acataagagc cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc
    37741 ggaacgtctg gatttcgaac tggcctgttg ctaaggagta aaaatgtcag aaactattac
    37801 ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc
    37861 attttgctcc aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg
    37921 aatccccagc agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg
    37981 acatttgtct gatgcctcac gtaggcctga taagacgcgg acagcgtcgc atcaggcatc
    38041 ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg
    38101 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgacc agcgacatcc
    38161 actcaccggg ttgcccttct ttaccccacg gccccccttc ccaactttcg accagccaga
    38221 accacagagt tatatgcctg tccgggaatt catattccag tttttcaaat agcgaaaaat
    38281 gttgtggggt aatcccgacc tcttcctgaa gttcacgcac caccgcctgt tccggcgttt
    38341 cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    38401 cgcgacgtgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    38461 gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac
    38521 tgttagcttt tattgcaggc gaccatggca ctgcttgtat tttttaccag aaccgcacgg
    38581 gcaaggatcg ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc
    38641 tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt gctaaacgct cggcttccat
    38701 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag
    38761 cgtactgata acttcatatt tcaacgactc cagcatcgct gcaaacatgg agaacgattc
    38821 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    38881 cagatagtcc atcgctgcca ggtgctcttt ccacagggag tcaagcgttt gcagcatgac
    38941 gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    39001 ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    39061 atccagccac tcggtaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    39121 ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtggc
    39181 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    39241 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    39301 cagttgctta cgaatgtcga agttacgact ttcaacttta cgctgggcgt tggcaatcgc
    39361 tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat
    39421 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    39481 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg
    39541 acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc
    39601 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag
    39661 cgcggcaact tctgcctgcc agctacctcc gagcacaata tctgtaccac gacccgccat
    39721 gttggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    39781 ggcgtggaat ttggcgttca ggacattgtg cttaataccg gctttggtca gttcgtttga
    39841 caccagctcc gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt
    39901 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    39961 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagttt
    40021 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    40081 ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg
    40141 gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgtcggcc
    40201 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    40261 gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    40321 catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag
    40381 cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc
    40441 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    40501 gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    40561 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    40621 atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatgttgt cgcgcaggta
    40681 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt
    40741 tgccggtgct ggcatgcccg gcaggttgat accaacagtc aggccaagga attcaaacag
    40801 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    40861 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    40921 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    40981 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc
    41041 cgggatcaga ttttccagca cttcgccttt ttccagacgc gcacgaaact ctgcggtttt
    41101 ccctttcagt tcttcatcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    41161 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    41221 caatttgatt agcataataa aatctcaaac gccccgcgtt acggagttaa taaaatgaag
    41281 taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc
    41341 ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat
    41401 aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg
    41461 caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca
    41521 ttgcgaaaga aagatgacgg attaccgtac gaatagcatg ttgatgccag taatcaacgg
    41581 aatagttcgg attcgggcgg cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa
    41641 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg
    41701 cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc
    41761 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    41821 cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca
    41881 gcacgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt
    41941 ttatagctct tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca
    42001 gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta cagctgaagg
    42061 cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc
    42121 ctgtttcgcc aggacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc
    42181 ggtaaatgca ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat
    42241 tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc
    42301 gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata
    42361 ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc
    42421 agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc
    42481 gatggtgaaa tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc
    42541 ttcgacacga acagtctctt tgatgcgaac aaattttttg gcgcagttca actcgtcgat
    42601 accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc
    42661 cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag
    42721 gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt
    42781 atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg
    42841 atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt
    42901 atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt
    42961 attatctcgc caaattacct atccaaccga agtgtactat acattcggcg ggccagttta
    43021 gcacaaagag cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgtagga
    43081 atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacta
    43141 ctttagcaac cggcttctgc tcctgggtca gcggagccat cccatgctgc tggtagcgat
    43201 ccatcactgg ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca
    43261 tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc attcatatcc gggtcaagag
    43321 aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg
    43381 tttcgaactc atccagacgc aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg
    43441 cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt
    43501 ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc atttcagaca
    43561 ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcgg
    43621 cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg
    43681 agataccgcg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca
    43741 catgcttgga cagttcagtg atcccctgct cggcgaatgc catacgcttc ttgccttcaa
    43801 agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag
    43861 cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca
    43921 tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc gcattgcggc
    43981 caacttctgg attagcgcca gcgcccagtc ctttggtgat accgctaccg atttgaatcg
    44041 tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa
    44101 caccttcaat acgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc
    44161 cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc
    44221 gatttgtgcc tgtcgcctga gtccgtaatc atcgtcggcc tcataaaaat taaaactctt
    44281 ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt
    44341 ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg
    44401 tcgaataata cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga
    44461 tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg
    44521 cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct
    44581 tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag caactcggta tagcgcggct
    44641 cgatcacctc tgccagggtc tgacgttgca gactccgcgg cggacgaccg cctacgctcg
    44701 gcacttccac gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt
    44761 taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga
    44821 cattgccagc ataaggaatt acctttgtgt ggcgcaatgc cccaccggta taaacggcga
    44881 tatccattgt accaccaccg atatcgacga cgcagacacc cagttcacgt tcatcttccg
    44941 tcaataccga ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac
    45001 aacgttcaac cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca
    45061 cttttgcctg catccgcaca cccgaaagtc ctaccggatt cttgatccct tcctgatagt
    45121 caatcgcata ctcttgcggg atcacatgca gcacacgatg ctcatcgcgc acacgcaccg
    45181 atttcgcggt atggacgacg ttttccacat cttcttgcgt cacttcttct tcagaaatag
    45241 gcaccatacc aatttcattc tggcagctga tgtgcttacc agaaagcgcc agatataccg
    45301 aagagatctg acaatctgcc atcaattctg cctggtcaat ggcgcgttgt acgcacttga
    45361 ccacggattc gaggtcgttc accccgcctt tatccatacc acgtgacggg cagctgccca
    45421 cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg
    45481 cggtaccaat ttccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc
    45541 tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac
    45601 tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc
    45661 ctgctgctgt aaaaccggat aaagttctac aaagcgaacc aaacgtttca tcgtatcgcc
    45721 ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt
    45781 catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata
    45841 gccctgcaac acttcattag cgctgccttc cgggccatac agcattggaa gcacctgctt
    45901 gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca tgtgttgatc
    45961 attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg
    46021 ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat
    46081 gttgacatcc tgggtcataa aggtacccgg ctcacccaat gccaggatcg actgccggat
    46141 atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg
    46201 ttgcgcatct tccatccagc tcaacacgac ccagccgctc accaacactg tcgttaaaac
    46261 ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc
    46321 ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa
    46381 ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc
    46441 tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt ccatcactgt
    46501 ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca
    46561 ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg
    46621 tctcatcaga gagatacttc gcctcataat catagaaggt tccggacggt tgaatacgta
    46681 ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt
    46741 tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat
    46801 tttctgctac tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa
    46861 ccggcaaacc cagagcagaa atttctgcta actgcttatc gctcaggcct ttttcaaact
    46921 ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccttgc cacagaagtt
    46981 tgctgcgtag tttatctatt gaaagcgcag atgccatcac tccgctgccg gtataaggca
    47041 agcccatcag ctcgagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg
    47101 cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt
    47161 cgacaggata cgcatcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat
    47221 tcagagaaac ttcccgctca gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca
    47281 tgttgttctt cctccggagt ttgcggcttc agtttgattt cagctaaaga acgggcaatt
    47341 tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggtgcc
    47401 agcatctcgg ctacctgcgc agggtccggc accagaatgg gatcaatttt cccacgtcca
    47461 cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga
    47521 tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac
    47581 aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc
    47641 cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg gccgtagtca
    47701 tcgaccagca ttgccgtacc gcttttacca ttcactggct ccagcgggaa ttcaccgagg
    47761 aaatcaaaac ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg
    47821 tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg
    47881 cctggcgcat tcagggtgac gcacatcggc tctttgtcct gacgcagcag cgtaaagtgc
    47941 ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg
    48001 taagttgtgg tctgacgtcc cacacgcggt aacaattcgc ggatcaccgg atcatcaaca
    48061 cacatcaccg cacgaccgta aaaaggcagg ttgtgcagaa aattaataaa ggtctgtttt
    48121 aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc
    48181 gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg
    48241 taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagccc gccgttaacg
    48301 aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt agttgtcgtt
    48361 ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt
    48421 tcggcacgac ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttgtcg
    48481 gcagaaatgg cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg
    48541 ttgaaataaa tcgtcgcacc cagattcagt aactgctgcg tgaccggatt tggcgctaaa
    48601 tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg
    48661 gcaccaccaa tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa
    48721 cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac
    48781 gatgcaaaag gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc
    48841 gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt
    48901 ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcga
    48961 taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt
    49021 tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcacagtta
    49081 aagcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa
    49141 taaattcagt cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt
    49201 gttgcgaacc tttgccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa
    49261 cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact accaacacac
    49321 gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg
    49381 tacgcaccgg gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca
    49441 ctttggtagc aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat
    49501 gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc
    49561 cgcccatacc gagtaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc
    49621 aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcaaac
    49681 cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac
    49741 ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatag tgcgcaaccg
    49801 ccagtcccgg gaatacatgt ccaccggttc cgcctgccat caccattaat cgctttcctt
    49861 gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc
    49921 aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact
    49981 gattagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac
    50041 cagcgcctgg aagctaaacc agatgccaat agaacaggcg agaaaaccgg aaaaacggtg
    50101 gtcaatttct aatgctttgc ggccaatcga catcgcacga aaagcgacga agaataccat
    50161 taaaagtgct agtaccacac cgacataacc taattcttcg ccgataatgg cgaaaataaa
    50221 gtcagtgtgc gcttccggca gatactccag tttttgtacc gaattaccta aaccttgccc
    50281 ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt aactgatagc cgctgccaaa
    50341 gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag
    50401 gatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt
    50461 cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag
    50521 gtctggctgt gccagcagta acactgccaa caccagaatc acgcccatcg gtttcaggaa
    50581 gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca
    50641 aaacagcgac agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca
    50701 acgcgatgcc cctttaacag cgctacccac taccagcacg atcatcagca ggatgataga
    50761 tccgagcagc atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat
    50821 gatcgccaga ataaacgcca aaatcagata gacaccatca cgcttcgcga agaagaacgg
    50881 atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat
    50941 cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt
    51001 atctttttcc cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccaggat
    51061 actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac
    51121 tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg
    51181 ctggcacagg ccggggagag cagaaccata tcgcccggct gaacacgcgg agccagcaag
    51241 cgcatagcct gttccatagt ttcggtttgt tctgccactt ccgggcgtag cgccgccagc
    51301 tgcgcgccgt cacgaccgaa acaatacaga cgtacgttat cgccattcag gtaacgcacc
    51361 agtgggctaa agtccgccga tttaccatcg ccgcccagca acaaatgcag cgtgccgtct
    51421 acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta
    51481 atccagcgca caccgttatg ctccagcacc acttcaaagc gatgcggcag accggtgaag
    51541 gtggttaacg ctttcaggct gctggcgcgc ggtaaccctg cggcatctgc cagcgccagc
    51601 gccaccagcg cattggtgta gttatgctgc ccggaaagtt tcatctcttt cacattcagc
    51661 actttctcgc ctttaacccg cagccaggtt tcgccctgct gatgattcag gtgatagtca
    51721 cccatgttga cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag
    51781 gcatcatcag cattaaccac gcaaactttc gcgttttcgt aaatgcgcag ttttgctgca
    51841 cgatactgtt gtaaaccaaa cgggtagcga tccatatgat cttcagtcac gttcagaatg
    51901 gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct ggaagctcga cagttccagc
    51961 acgtacagtt cacactcatc atccagtagc atcaacgcag gcaggccaat attgccaccc
    52021 acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt
    52081 ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca aaacagctcg
    52141 atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt
    52201 gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc attcagactg
    52261 cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta
    52321 tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga aagcccggtg
    52381 aggcccaggc cgataatgac aacattttta ccctggtaat cagccatgat taacgtacct
    52441 tcagcgttgc cagaccaatc aggaccagca tcagcgaaat aatccagaaa cgcacaatga
    52501 cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa
    52561 tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacagaaagc gtttctacca
    52621 cgaacacgcc ccccataatc accagcagga attcctgacg tagcagtacg gcgataatgc
    52681 ctaacgcacc acctaacgcc agcgaaccga catcgcccat aaagacctgc gccggatagg
    52741 tgttaaacca caggaagccc agtcctgccc cgactatcgc ggtacagaca ataaccagtt
    52801 ccccggcgtg tcgcagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg
    52861 cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccagaccgt
    52921 caagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacgaag taagccagca
    52981 gaatgtagaa cagccccagt tgcggcatca catctttaaa gaatgggacc accagctgcg
    53041 ttgcgggcgt gtctttgccg gcaaggtaca aagcgaaagc aacacccagc gcaatgaccg
    53101 acatccagaa atatttccaa cgagcgatca accctttggt gtctttacgc accactttgc
    53161 gataatcatc aacaaagcca ataacaccgt aacctaccag caccaccaac acgcaccaga
    53221 cgtacggatt ggacgggtaa gcccacagca gcacggagat cacaatcgct gtcaggatca
    53281 taatcccgcc catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac
    53341 gcactacctg accaaaggaa agtttttgca aatgagcaat catgcgcggg cccatccaca
    53401 atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga
    53461 cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt
    53521 ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta cgtgaaccct taactaaaat
    53581 cgtaattacc tgttgctcag caatcagtga tttaagacgc gtaattaacg cagttttatc
    53641 agcaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccatgct
    53701 taacacgcgg tcaataccag cagcttttgc cgcctcaccc acctgtacat ggcaggcttc
    53761 gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc
    53821 agccagtacc tgaacagctg cggtcattga accgacattg gcgttgtagg agtcgtcgag
    53881 cagcaactgg ttttctgcca gttggatggg gaacagacgg ccaggaacag ctttcagatt
    53941 tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc
    54001 tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc
    54061 ggttggggtt tgtagggtaa attccatacc gtgcgaggtc acatggatat tggtggcggt
    54121 gaaatcgctg ttggcggcat tgggtgagaa acgccacact ttgcgtgagc caattacgct
    54181 ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag
    54241 gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag
    54301 atgcgccgct gccaggttgt tgaccagcgc cgcttccggg cgagtcagac tcacagtcca
    54361 ggcaatctcg ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt
    54421 taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag
    54481 cgtgttgccg cactggctta aaatcgccgc cgtcatctct ttaacggagg ttttgccgga
    54541 ggaccccgtc agagcaacca cgagcgccgg aacttgctgg cgaacccatg cagccagttc
    54601 accaaacgcc agacgcgtat ccttgacgat taactgcggc aggtcgatgt ccagcggacg
    54661 gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg tcggcaaaat cgtgggcatc
    54721 aaaacgttca cctttcaggg caacaaacag gcagcccggc gtcagttttc gggtatcagt
    54781 ggttacagca tcaagggtga tatctgcacc ttgcagttca ccgttgagaa tatcggtaag
    54841 ttggctaagg gttacgctaa tcatgcaatc ccccccagca gacgcgccac cgtgacgcga
    54901 tcggagtagt ccagacgctg attgccaaca atctggtaat cctcatggcc tttgcccgcg
    54961 accagtacca catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg
    55021 ccttccatca ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg
    55081 gcacgcggtt cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct
    55141 tcggcaattg cgcccatcag tggacgctta cctttatcgc gatcgccacc acagccaaag
    55201 acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca
    55261 tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctggcgcagt gaacacttcc
    55321 atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcagatcagc cagtggatag
    55381 cccagcgcca gcagtgttgc cagcgcgagc aacaggttgc tgacgttaaa agcgcccatc
    55441 agatggcttt caatttcgcc atcgccccag cttgaactaa agcgaatggt cgcgccgctg
    55501 tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt gacagttcgg attgatatga
    55561 tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca
    55621 tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag aataaagcag ccatttcgca
    55681 gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag
    55741 accgacgccg gaaatttcaa tgccgccaca cggtgctgta ccagcccgtg ggaggaaact
    55801 tccattgcgc aaaaagtcgc gccctgatcc accagccccg ccagctcatg ctgaacatca
    55861 actgccgaac cggtagtatt ttctgtcggg atcactttcc ccagcagacc gttaccaacg
    55921 gtgcccatta ccgcgctggt ttcgccaagc agttggctcc actgcgccag aagctgggtc
    55981 gtcgtggttt tgccgttggt gcccgttacg cctacgagac gtaaattgtc agagggttca
    56041 tggtaaaagc ggcccgccag tgcagataaa cgctcgttga gctggctgag atagatgacc
    56101 ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata
    56161 atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga
    56221 cctactacag ctacaaagag atcgcccgcc gcagccacac ggctgtcgag tgtcatctct
    56281 cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga
    56341 tctgccacct gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag
    56401 cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg
    56461 cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag
    56521 cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat
    56581 gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc
    56641 tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc acgctttcca tcatatgcac
    56701 cacggtgcga acaatggatt ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt
    56761 ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa
    56821 ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga
    56881 ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc
    56941 ttttcccagt ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgttaacgc
    57001 cagcttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct
    57061 gtagcgtgcc acgtctttga tttcgtggcc gttaattcga taaggaatgg tattgagtac
    57121 cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt
    57181 tgagcccggt tcaaacacgt cggtgatggt acggttacgc atcgcctctt tcggcgtgcc
    57241 gctcagattg ttagggttgt atgacgggct gttagccatc gccagcactt cacctgtgtt
    57301 gacatccacc agcacggcgc taccagattc agccttgtta aaggccaccg cgttgttcag
    57361 ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc
    57421 ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat
    57481 gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcaacgc cctcaatccc
    57541 ttgactatcg acgttagtaa agccgatgag gtgagcagtc acttcgccgg acggatagta
    57601 acggcgagac tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc
    57661 catgtcagag tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat
    57721 gcgggcagaa agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcggtc
    57781 accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac
    57841 cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga
    57901 aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat
    57961 aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca
    58021 taacaacgca aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg
    58081 cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc
    58141 ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct
    58201 atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc
    58261 tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt
    58321 taccacggtc accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa
    58381 tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga
    58441 tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgca
    58501 atacgcagaa ctgaactacg ggcacgaggg ttctcagcca cctcttcttc gcccggcatt
    58561 aacttgccta gtgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc
    58621 ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata
    58681 cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg
    58741 agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc
    58801 gcctggaagg tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcagcagcc
    58861 acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg
    58921 gcgcgggcaa tgcgtttggc aaaatgctct tcaccatagg ttttcaatac ccaggcgata
    58981 tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata
    59041 cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc gttcagcatc atcaagttgc
    59101 ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccaataag atcgcgctcg
    59161 gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca
    59221 atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct
    59281 tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg
    59341 tagatgccat caggacggat attgaggcca ttaacggctt catccagcag caccgtagta
    59401 tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc
    59461 ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc
    59521 ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg
    59581 ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc
    59641 atgacctaac agtaggcgct gcacacggcg ctcaaccggg ttcatgctca acagacgcga
    59701 taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg
    59761 aatgtcaatg gtgcaaacca tttgaccggc agcgttctca agcagctgtt cccgataacg
    59821 ggtaggcact gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat
    59881 gccagcctca ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg
    59941 agcggaggaa aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt
    60001 tacggctaat atcagcgttg tttattttac gtccaacgtg cgaagcaaat taacgttatg
    60061 aatagctgta agaaaaaatc cctcatcgtc atcactgttt taaaatgact cactattttc
    60121 cacagcaaat ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttcccc
    60181 ggttaacagt ccttgttacg tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca
    60241 aagaaaagtg tttgttaaat cggcaattca tcgccgcatg taacaaaata atgcaaaaac
    60301 gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg tgagttaatt aacgggcggg
    60361 tttaaaacca tttatcaata atagtcagga atagtcttat ttactttaa
//
LOCUS       DAZPAB010000004        58198 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZPAB010000004 DAZPAB010000000
VERSION     DAZPAB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.004"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            115..2649
                     /gene="mrcB"
                     /locus_tag="ACHUPG_000211"
     CDS             115..2649
                     /gene="mrcB"
                     /locus_tag="ACHUPG_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR0859984.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            2869..5112
                     /gene="fhuA"
                     /locus_tag="ACHUPG_000212"
     CDS             2869..5112
                     /gene="fhuA"
                     /locus_tag="ACHUPG_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR0859985.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            5163..5960
                     /gene="fhuC"
                     /locus_tag="ACHUPG_000213"
     CDS             5163..5960
                     /gene="fhuC"
                     /locus_tag="ACHUPG_000213"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR0859986.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            5960..6850
                     /gene="fhuD"
                     /locus_tag="ACHUPG_000214"
     CDS             5960..6850
                     /gene="fhuD"
                     /locus_tag="ACHUPG_000214"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR0859987.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            6847..8829
                     /gene="fhuB"
                     /locus_tag="ACHUPG_000215"
     CDS             6847..8829
                     /gene="fhuB"
                     /locus_tag="ACHUPG_000215"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR0859988.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8864..10144)
                     /gene="hemL"
                     /locus_tag="ACHUPG_000216"
     CDS             complement(8864..10144)
                     /gene="hemL"
                     /locus_tag="ACHUPG_000216"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR0859989.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            10310..10357
                     /gene="yadX"
                     /locus_tag="ACHUPG_000217"
     CDS             10310..10357
                     /gene="yadX"
                     /locus_tag="ACHUPG_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR0859990.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            10369..11790
                     /gene="clcA"
                     /locus_tag="ACHUPG_000218"
     CDS             10369..11790
                     /gene="clcA"
                     /locus_tag="ACHUPG_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR0859991.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            11814..11879
                     /gene="yadW"
                     /locus_tag="ACHUPG_000219"
     CDS             11814..11879
                     /gene="yadW"
                     /locus_tag="ACHUPG_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR0859992.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            11872..12216
                     /gene="erpA"
                     /locus_tag="ACHUPG_000220"
     CDS             11872..12216
                     /gene="erpA"
                     /locus_tag="ACHUPG_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR0859993.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(12263..12886)
                     /locus_tag="ACHUPG_000221"
     CDS             complement(12263..12886)
                     /locus_tag="ACHUPG_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR0859994.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(12924..13724)
                     /gene="btuF"
                     /locus_tag="ACHUPG_000222"
     CDS             complement(12924..13724)
                     /gene="btuF"
                     /locus_tag="ACHUPG_000222"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR0859995.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(13717..14415)
                     /gene="mtnN"
                     /locus_tag="ACHUPG_000223"
     CDS             complement(13717..14415)
                     /gene="mtnN"
                     /locus_tag="ACHUPG_000223"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR0859996.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            14499..16016
                     /gene="dgt"
                     /locus_tag="ACHUPG_000224"
     CDS             14499..16016
                     /gene="dgt"
                     /locus_tag="ACHUPG_000224"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR0859997.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            16146..17570
                     /gene="degP"
                     /locus_tag="ACHUPG_000225"
     CDS             16146..17570
                     /gene="degP"
                     /locus_tag="ACHUPG_000225"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR0859998.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            17725..18881
                     /gene="cdaR"
                     /locus_tag="ACHUPG_000226"
                     /pseudo
     CDS             17725..18881
                     /gene="cdaR"
                     /locus_tag="ACHUPG_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(18970..19356)
                     /locus_tag="ACHUPG_000227"
     CDS             complement(18970..19356)
                     /locus_tag="ACHUPG_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR0859999.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(19670..20494)
                     /gene="dapD"
                     /locus_tag="ACHUPG_000228"
     CDS             complement(19670..20494)
                     /gene="dapD"
                     /locus_tag="ACHUPG_000228"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR0860000.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(20525..23197)
                     /gene="glnD"
                     /locus_tag="ACHUPG_000229"
     CDS             complement(20525..23197)
                     /gene="glnD"
                     /locus_tag="ACHUPG_000229"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR0860001.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(23259..24053)
                     /gene="map"
                     /locus_tag="ACHUPG_000230"
     CDS             complement(23259..24053)
                     /gene="map"
                     /locus_tag="ACHUPG_000230"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR0860002.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            24421..25146
                     /gene="rpsB"
                     /locus_tag="ACHUPG_000231"
     CDS             24421..25146
                     /gene="rpsB"
                     /locus_tag="ACHUPG_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR0860003.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            25404..26255
                     /gene="tsf"
                     /locus_tag="ACHUPG_000232"
     CDS             25404..26255
                     /gene="tsf"
                     /locus_tag="ACHUPG_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR0860004.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            26402..27127
                     /gene="pyrH"
                     /locus_tag="ACHUPG_000233"
     CDS             26402..27127
                     /gene="pyrH"
                     /locus_tag="ACHUPG_000233"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR0860005.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            27419..27976
                     /gene="frr"
                     /locus_tag="ACHUPG_000234"
     CDS             27419..27976
                     /gene="frr"
                     /locus_tag="ACHUPG_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR0860006.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            28068..29264
                     /gene="ispC"
                     /locus_tag="ACHUPG_000235"
     CDS             28068..29264
                     /gene="ispC"
                     /locus_tag="ACHUPG_000235"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR0860007.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            29453..30211
                     /gene="ispU"
                     /locus_tag="ACHUPG_000236"
                     /gene_synonym="uppS"
     CDS             29453..30211
                     /gene="ispU"
                     /locus_tag="ACHUPG_000236"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR0860008.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            30224..31081
                     /gene="cdsA"
                     /locus_tag="ACHUPG_000237"
     CDS             30224..31081
                     /gene="cdsA"
                     /locus_tag="ACHUPG_000237"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR0860009.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            31093..32445
                     /gene="rseP"
                     /locus_tag="ACHUPG_000238"
     CDS             31093..32445
                     /gene="rseP"
                     /locus_tag="ACHUPG_000238"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR0860010.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            32475..34907
                     /gene="bamA"
                     /locus_tag="ACHUPG_000239"
     CDS             32475..34907
                     /gene="bamA"
                     /locus_tag="ACHUPG_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR0860011.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            35029..35514
                     /gene="skp"
                     /locus_tag="ACHUPG_000240"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             35029..35514
                     /gene="skp"
                     /locus_tag="ACHUPG_000240"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR0860012.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            35518..36543
                     /gene="lpxD"
                     /locus_tag="ACHUPG_000241"
     CDS             35518..36543
                     /gene="lpxD"
                     /locus_tag="ACHUPG_000241"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR0860013.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            36648..37103
                     /gene="fabZ"
                     /locus_tag="ACHUPG_000242"
     CDS             36648..37103
                     /gene="fabZ"
                     /locus_tag="ACHUPG_000242"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR0860014.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            37107..37895
                     /gene="lpxA"
                     /locus_tag="ACHUPG_000243"
     CDS             37107..37895
                     /gene="lpxA"
                     /locus_tag="ACHUPG_000243"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR0860015.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            37895..39043
                     /gene="lpxB"
                     /locus_tag="ACHUPG_000244"
     CDS             37895..39043
                     /gene="lpxB"
                     /locus_tag="ACHUPG_000244"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR0860016.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            39040..39636
                     /gene="rnhB"
                     /locus_tag="ACHUPG_000245"
     CDS             39040..39636
                     /gene="rnhB"
                     /locus_tag="ACHUPG_000245"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR0860017.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            39673..43155
                     /gene="dnaE"
                     /locus_tag="ACHUPG_000246"
     CDS             39673..43155
                     /gene="dnaE"
                     /locus_tag="ACHUPG_000246"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR0860018.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            43168..44127
                     /gene="accA"
                     /locus_tag="ACHUPG_000247"
     CDS             43168..44127
                     /gene="accA"
                     /locus_tag="ACHUPG_000247"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR0860019.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            44226..46367
                     /gene="ldcC"
                     /locus_tag="ACHUPG_000248"
     CDS             44226..46367
                     /gene="ldcC"
                     /locus_tag="ACHUPG_000248"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR0860020.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            46424..46813
                     /locus_tag="ACHUPG_000249"
     CDS             46424..46813
                     /locus_tag="ACHUPG_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0860021.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            46878..48176
                     /gene="tilS"
                     /locus_tag="ACHUPG_000250"
     CDS             46878..48176
                     /gene="tilS"
                     /locus_tag="ACHUPG_000250"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR0860022.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(48225..48485)
                     /gene="rof"
                     /locus_tag="ACHUPG_000251"
     CDS             complement(48225..48485)
                     /gene="rof"
                     /locus_tag="ACHUPG_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR0860023.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(48472..48672)
                     /locus_tag="ACHUPG_000252"
     CDS             complement(48472..48672)
                     /locus_tag="ACHUPG_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR0860024.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            48838..49383
                     /locus_tag="ACHUPG_000253"
     CDS             48838..49383
                     /locus_tag="ACHUPG_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR0860025.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            49380..49802
                     /gene="arfB"
                     /locus_tag="ACHUPG_000254"
     CDS             49380..49802
                     /gene="arfB"
                     /locus_tag="ACHUPG_000254"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFR0860026.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            49816..50526
                     /gene="nlpE"
                     /locus_tag="ACHUPG_000255"
                     /gene_synonym="cutF"
     CDS             49816..50526
                     /gene="nlpE"
                     /locus_tag="ACHUPG_000255"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR0860027.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(50681..51505)
                     /locus_tag="ACHUPG_000256"
     CDS             complement(50681..51505)
                     /locus_tag="ACHUPG_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR0860028.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(51558..53276)
                     /gene="proS"
                     /locus_tag="ACHUPG_000257"
     CDS             complement(51558..53276)
                     /gene="proS"
                     /locus_tag="ACHUPG_000257"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR0860029.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(53387..54094)
                     /gene="tsaA"
                     /locus_tag="ACHUPG_000258"
     CDS             complement(53387..54094)
                     /gene="tsaA"
                     /locus_tag="ACHUPG_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR0860030.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(54091..54495)
                     /gene="rcsF"
                     /locus_tag="ACHUPG_000259"
     CDS             complement(54091..54495)
                     /gene="rcsF"
                     /locus_tag="ACHUPG_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR0860031.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(54613..55428)
                     /gene="metQ"
                     /locus_tag="ACHUPG_000260"
     CDS             complement(54613..55428)
                     /gene="metQ"
                     /locus_tag="ACHUPG_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR0860032.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(55468..56121)
                     /locus_tag="ACHUPG_000261"
     CDS             complement(55468..56121)
                     /locus_tag="ACHUPG_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR0860033.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(56114..57145)
                     /gene="metN"
                     /locus_tag="ACHUPG_000262"
     CDS             complement(56114..57145)
                     /gene="metN"
                     /locus_tag="ACHUPG_000262"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR0860034.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            57333..57908
                     /gene="gmhB"
                     /locus_tag="ACHUPG_000263"
     CDS             57333..57908
                     /gene="gmhB"
                     /locus_tag="ACHUPG_000263"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR0860035.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        13822 a        14344 c        16001 g        14031 t
ORIGIN      
        1 ccctcaaggg agaggggacc gatcgagcgc aaattttgag agatatcttc ttctgtcttg
       61 taacagaaga acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc
      121 gggaatgacc gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag
      181 gtaagccgtc gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat
      241 gaagaaccga tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa
      301 cgcggctggc tatggctact gctaaaactg gctatcgttt ttgccgtgct gatcgccatt
      361 tacggcgttt atctcgatca aaaaattcgt agccgtattg atggcaaggt ctggcaactg
      421 cccgcggcag tttatggccg aatggtcaac cttgaaccag atatgaccat cagcaagaac
      481 gaaatggtga agctgctgga ggcaacacag tatcgtcagg tgtcgaaaat gacccgtcct
      541 ggcgaattta ccgtgcaggc caacagcatt gagatgatcc gccgtccgtt tgatttcccg
      601 gacagtaaag aagggcaggt gcgtgcgcgt ctgacctttg atggtgatca tctggcgacc
      661 atcgtcaata tggagaacaa ccgtcagttc ggtttcttcc gtcttgatcc gcgtctgatc
      721 accatgatct cctcgccaaa cggtgagcag cgtttgtttg tgccgcgcag tggtttccct
      781 gatttgctgg tggatacttt gctggcgaca gaagaccgtc atttttacga gcatgatgga
      841 atcagtctct actccattgg gcgtgcggtg ctggcaaacc tgaccgcagg tcgcacggtg
      901 cagggtgcga gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc cagcgagcgt
      961 tcttactggc gtaaagcgaa cgaagcttac atggcgctga tcatggacgc gcgttacagc
     1021 aaagaccgta ttcttgagct gtatatgaac gaggtgtatc tcggtcagag cggcgacaac
     1081 gaaatccgcg gcttcccgct ggcaagcttg tattactttg gtcgcccggt agaagagcta
     1141 agcctcgacc agcaggcgct gttagtcggt atggtgaaag gggcgtccat ctacaacccg
     1201 tggcgtaacc cgaaactggc gctggagcga cgtaatctgg tgctgcgtct gctgcaacag
     1261 caacagatta ttgatcaaga actctatgac atgttgagtg cccgtccgct gggggttcag
     1321 ccgcgtggtg gggtgatctc tcctcagcca gcctttatgc agctggtgcg tcaggagttg
     1381 caggcaaaac tgggcgataa ggtaaaagat ctctccggcg tgaagatctt cactaccttt
     1441 gactcggtgg cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag
     1501 aaacagcgta agttgagcga tcttgaaact gcgattgtgg tcgtcgaccg ctttagtggt
     1561 gaagttcgtg cgatggtcgg aggttctgag ccgcagtttg cgggctacaa ccgtgcgatg
     1621 caggcgcgtc gttcgattgg ttcccttgca aaaccagcga cttatctgac ggctttaagc
     1681 cagccgaaaa tctatcgcct gaacacgtgg attgccgatg cgccaatcgc gctgcgtcag
     1741 ccgaatggcc aggtctggtc accgcagaac gatgaccgtc gttatagcga aagcggcaga
     1801 gtgatgctgg tggatgcgtt gacacgttcg atgaacgtgc cgacggtaaa tctggggatg
     1861 gcgctggggc tgcctgcggt tacggagacc tggatcaaac tgggcgtacc gaaagatcag
     1921 ttgcatccgg ttccggcaat gctgctgggg gcgttgaact taacgccaat cgaagtggcg
     1981 caggcattcc agaccatcgc cagcggtggt aaccgtgcac cgctttctgc actgcgttcg
     2041 gtaatcgcgg aagatggtaa ggtgctgtat cagagcttcc cgcaggcaga acgcgctgtt
     2101 ccggcgcagg cggcgtatct gacactatgg accatgcagc aggtggtaca acgtggtacg
     2161 ggtcgtcagc ttggggcgaa atacccgaac ctgcatctgg cagggaaaac agggactacc
     2221 aacaataacg tagatacctg gtttgcaggc attgacggca gcacggtgac catcacctgg
     2281 gtaggccgtg ataacaacca gccgaccaaa ctgtatggag ccagcggggc aatgtcgatt
     2341 tatcagcgtt atctggctaa ccagacgcca acgccgctga atcttgttcc gccagaagat
     2401 attgcagata tgggcgtgga ctacgacggc aactttgttt gcagcggtgg catgcgtgtc
     2461 ttgccggtct ggaccagcga tccgcaatcg ctgtgccagc agagcgagat gcagcagcag
     2521 ccgtcaggca atccgtttga tcagtcttct cagccgcagc aacagccgca acagcaacct
     2581 gctcagcaag agcagaaaga cagcgacggt gtagccggtt ggatcaagga tatgtttggt
     2641 agtaattaac atctaagcgt gaaataccgg atggcgagtt gccatccggt aaaataacat
     2701 cccatctaag atattaaccc tttcttttca tctggttgtt tattaaccct tcaggaacgc
     2761 tcagattgcg taccgcttgc gaacccgcca gcgtttcgaa tattatctta tctttataat
     2821 aatcattctc gtttacgtta ttattcactt tacatcagag atataccaat ggcgcgttcc
     2881 aaaactgctc agccaaaaca ctcactgcgt aaaatcgcag ttgtagtagc cacagcggtt
     2941 agcggcatgt ctgtttatgc acaggcagcg gttgaaccga aagaagacac tatcaccgtt
     3001 accgctgcac ctgcgccgca agaaagcgca tgggggcctg ctgcaactat tgcggcgcgg
     3061 cagtcagcta ccggcactaa aaccgatacg ccgattcaaa aagtgccaca gtctatttct
     3121 gttgtgaccg ccgaagagat ggcgctgcat cagccgaagt cggtaaaaga agcgcttagc
     3181 tacacgccgg gtgtctctgt tggtacgcgt ggcgcatcca acacctatga ccacctgatc
     3241 attcgcggtt ttgcggcaga aggccaaagc caaaataact atctgaatgg cctgaagttg
     3301 cagggcaact tctataacga tgcggtcatt gacccgtata tgctggaacg cgctgaaatt
     3361 atgcgtggcc cggtttccgt gctttacggt aaaagcagtc ctggcggtct gttgaatatg
     3421 gtcagcaagc gtccgaccac cgaaccgctg aaagaagttc agtttaaagc cggtactgac
     3481 agcctgttcc agactggttt tgactttagc gatgcgttgg atgatgacgg tgtttactct
     3541 tatcgcctga ccggtcttgc gcgttctgcc aatgcccagc agaaagggtc agaagagcag
     3601 cgttatgcta ttgcaccggc gttcacctgg cgtccggatg ataaaaccaa tttcaccttc
     3661 ctttcttact tccagaacga gccggaaacc ggttattacg gctggttgcc gaaagaggga
     3721 accgttgagc cgctgccgaa cggtaagcgt ctgccgacag actttaatga aggggcgaag
     3781 aacaacacct attctcgtaa tgagaagatg gtcggctaca gcttcgatca cgaatttaac
     3841 gacaccttta ctgtgcgtca gaacctgcgc tttgctgaaa acaaaacctc gcaaaacagc
     3901 gtttatggtt acggcgtctg ctccgatccg gcgaatgctt acagcaaaca gtgtgcggca
     3961 ttagcgccag cggataaagg ccattatctg gcacgtaaat acgtcgttga tgatgagaag
     4021 ctgcaaaact tctccgttga tacccagttg cagagcaagt ttgccactgg cgatatcgac
     4081 cacaccctgc tgaccggtgt cgactttatg cgtatgcgta atgacatcaa cgcctggttt
     4141 ggttacgacg actctgtgcc actgttcaat ctgtacaatc cggtgaatcc cgatttcgac
     4201 ttcaatgcca aagatccggc aaactccggc ccttaccgca ttctgaataa acagaaacaa
     4261 acgggcgttt atgttcagga tcaggcgcag tgggataaag tgctggtcac cctaggcggt
     4321 cgttatgact gggcagatca agaatctctt aaccgcgttg ctgggacgac cgataaacgt
     4381 gatgacaaac agtttacctg gcgtggtggt gttaactacc tgtttgataa tggtgtaaca
     4441 ccttacttca gctatagcga atcgtttgaa ccttcttcgc aagttgggaa ggatggtaat
     4501 attttcgcac cgtctaaagg taagcagtat gaagtcggcg tgaaatatgt accggaagat
     4561 cgtccgattg tagttactgg tgccgtgtat aatctcacta aaaccaacaa cctgatggcg
     4621 gaccctgagg gttccttctt ctcggttgaa ggtggcgaga tccgcgcacg tggcgtagaa
     4681 atcgaagcga aagcggcgct gtcggcgagt gttaacgtag tcggttctta tacttacacc
     4741 gatgcggaat acaccaccga tactacctat aaaggcaata cgcctgcaca ggtgccaaaa
     4801 cacatggctt cgctgtgggc tgactatacc ttctttgacg gtccgctttc aggtctgacg
     4861 ctgggcaccg gtggtcgtta tactggctcc agctatggtg atccggctaa ctcctttaaa
     4921 gtgggaagtt atacggtcgt ggatgcgtta gtgcgttatg atctggcgcg agtcggcatg
     4981 gcgggctcca acgtggcgct gcatgttaac aacctgttcg atcgtgaata cgtcgccagc
     5041 tgctttaaca cttatggctg cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc
     5101 tttcgtttct aatttctctt ttggggcacg gatttccgtg cccatttcac aagttggctg
     5161 ttatgcagga atacacgaat cattccgata ccacttttgc actgcgtaat atctcctttc
     5221 gtgtgcccgg gcgcacgctt ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga
     5281 ccggtctgat tggtcacaac ggttctggta aatccactct gctcaaaatg cttggtcgtc
     5341 atcagcctcc gtcagaaggg gagattcttc ttgatgccca gccgctggaa agctggagca
     5401 gcaaagcgtt tgcccgcaaa gtggcttatt tgccgcagca gcttcctccg gcagaaggga
     5461 tgaccgtgcg tgaactggtg gcgattggtc gttacccgtg gcatggcgcg ctggggcgct
     5521 ttggtgcggc agatcgcgaa aaggtcgagg aagctatctc gctggttggc ttaaaaccgc
     5581 tggcgcatcg gctggtcgat agtctctctg gcggcgaacg tcagcgggcg tggatcgcca
     5641 tgctggtggc gcaggatagc cgttgtctgt tgctcgacga accgacttca gcgctggata
     5701 tcgcccacca ggttgatgtg ctggcgctgg tgcaccgttt aagtcaggaa cgtggcctga
     5761 cggtcattgc cgtgttgcac gatatcaata tggcggcacg ctactgtgat tatctggtcg
     5821 ccctgcgcag tggtgaaatg attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa
     5881 ccctcgaaat gatttatggc atcccgatgg gtattttgcc gcatccggcg ggtgctgcac
     5941 ctgtgagttt tgtttattga tgagcggctt acctcttatt tcgcgccgtc gactgttaac
     6001 ggcgatggcg ctttctccgt tgttatggca gatgaatacc gcccacgcgg cggctattga
     6061 ccccaatcgt attgtggcgc tggagtggtt gccggtggaa ttactgctgg cgctcggcat
     6121 cgtgccttac ggcgtggcgg ataccatcaa ctatcgcctg tgggtcagcg aaccaccatt
     6181 gccggactca gtgattgacg tcggtttgcg tacagaacct aaccttgaac tgctgaccga
     6241 aatgaaaccg tcgtttatgg tctggtcggc aggatatggc ccttcatcag aaatgctggc
     6301 acgtattgcg ccgggtcgcg gatttaactt cagtgacggc aaacatccgt tggcgatggc
     6361 gcgtaaatcg ctgacggaaa tggcagattt acttaacctg caaagcgcag cggaaacgca
     6421 tttagcgcaa tacgaagact ttatccgcag catgaaaccc cgctttgtga agcgtggtgc
     6481 acgcccgtta ctgctgacga cgcttatcga tccgcgccat atgctggtct tcggtccaaa
     6541 cagcttgttc caggaaattc tcgatgagta cggcatccca aatgcctggc aaggggaaac
     6601 caacttctgg ggcagtaccg ccgtcagtat cgatcgtctg gcagcgtata aagacgttga
     6661 tgtgctctgt tttgatcacg acaacagcaa agacatggat gcgctaatgg caaccccgct
     6721 gtggcaggcc atgccgtttg tccgtacagg gcgctttcag cgcgtacccg cagtctggtt
     6781 ttatggtgca acgctttcgg caatgcactt tgtgcgtgtt ctggataacg ccatcggagg
     6841 taaagcgtga gtaaacgaat tgcgcttttc ccggcgttat tgctggcgct gttagtgatt
     6901 gtcgctacgg cgctcacctg gatgaacttc tcacaggcgc tgccgcgcag tcagtgggcg
     6961 caggccgcct ggtcgccgga tattgacgtc atcgagcaga tgatttttca ctacagcttg
     7021 ttgccgcgtc tggcgatttc gctgctggtg ggcgcgggcc tggggctggt gggcgtgctg
     7081 tttcagcaag tactgcgtaa cccgctggcg gaaccgacga cgttgggcgt tgcaacaggc
     7141 gcgcaactgg ggattaccgt caccacgctc tgggcgatcc ctagcgcgat ggcgagccag
     7201 tttgctgcgc tggcaggggc ttgcgttgtt ggcttaattg tctttggcgt cgcgtggggg
     7261 aaacggctgt cgccggtaac gctgattttc gcggggttgg tagtgagcct ttattgcggc
     7321 gcaatcaatc agttactggt tatcttccat catgaccagt tgcaaagcat gtttctgtgg
     7381 agcactggaa cgctgacgca aaccgactgg ggcggtgttg agcgtttatg gccgcagttg
     7441 ctgggcggtg tgatgctgac gttgctgcta cttcgtccgt taaccctgat ggggcttgat
     7501 gatggcgtgg cgcgcaatct cgggctggcc ttgtcgcttg cgcgtctggc ggcgctgtcg
     7561 ctggcgattg tcatcagtgc gctgctggtg aacgctgtgg ggattatcgg ctttatcggg
     7621 ttgttcgcgc cgctgctggc aaaaatgctg ggggcgcggc gtctgctgcc acgactgatg
     7681 ctggcgtcgt tgattggtgc gctgatcctc tggctttccg atcaaatcat cctctggctg
     7741 actcgcgtgt ggatggaagt gtccaccggt tcggtcactg cgttgatcgg tgcgccgctg
     7801 ctactgtggc tgctgccgcg tttacgcagc attagcgcgc cggatatgaa ggttaacgat
     7861 cgtgtcgcga ctgaacgcca acatgtgctg gcgtttgccc tcgcgggcgg cgtgctgttg
     7921 ttgatggctg tggtggtggc gctgtcgttt ggtcgtgatg cgcacggctg gacgtgggcg
     7981 agcggggcgt tgctcgagga tttaatgccc tggcgctggc cgcgaattat ggcggcgctg
     8041 tttgcgggcg tcatgctggc ggtagcgggc tgtattattc agcgactgac cggcaacccg
     8101 atggcaagcc cggaagtgct ggggattagc tccggcgcgg cgtttggtgt ggtgttaatg
     8161 ctgtttctgg taccgggtaa tgcctttggc tggttgttac ccgcaggcag tctcggggcg
     8221 gcggtgacgc tgttgatcat tatgatcgcc gccggacgcg gtggattttc cccacaccgc
     8281 atgttactgg cggggatggc gttaagtacc gcgttcacca tgcttttgat gatgttgcag
     8341 gcaagtggtg acccgcgaat ggcgcaagtg ctgacctgga tttccggttc gacctacaac
     8401 gcgaccgatg ctcaggtctg gcgcaccgga attgtgatgg tgattttgct ggcgattact
     8461 ccgctgtgcc gccgctggct gaccatttta ccgctgggtg gtgataccgc gcgggcggta
     8521 gggatggcgc tgacgccgac gcgaattgcg ctgctgctgt tagcggcttg cctgacggcg
     8581 accgcgacga tgactattgg accgttgagt tttgttggtt taatggcacc gcatattgcg
     8641 cggatgatgg gctttcgacg gacgatgcca cacatcgtaa tttcggcgct ggtgggtggt
     8701 ttactgctgg tgttcgctga ctggtgtggg cggatggtgc tgtttccatt ccagatcccg
     8761 gcggggctgc tgtcgacctt tatcggtgcg ccatatttta tctatttgct gagaaagcag
     8821 agccgttaat agaatgcctc gcccggtggg cgaggcaaaa ttatcacaac ttcgcgaaca
     8881 cccgacgtgc agcatcgatg gtgttattga tatcttccat gctgtgcgcc acagacataa
     8941 agcctgcttc aaacgctgac ggtgccaggt aaacaccttc atccagcatc atatggaaga
     9001 aacgcttaaa acgctcgacg tcacaggcca tcacatcctg atagcacgtc acggactcgg
     9061 cgtcggtaaa gaagataccg aacataccac cgacgtggtt aacgaccagc ggaattcccg
     9121 cttcttctgc cgcttccagc agaccttctg ccagacgtgt tgtcagctca tccagcgttt
     9181 cgtgaatgcc cggctgcgcg acttcattca gacaggcgaa acccgctgcc atcgcaatcg
     9241 ggttaccgga aagcgtaccc gcctgataga ccggacctgt cggggccagc gcatccatta
     9301 catcacgacg accaccgaat gcgcctaccg gcattccacc gccgatgatt tttccgaggc
     9361 aggttaaatc tggcactacg gcgaaataat cctgtgcgcc agctagcgct acgcggaaac
     9421 cggtcatcac ttcatcgatg atcagcaacg cgccaaattc gtcgcacagc gcacgcagac
     9481 ctggcaggaa ctctggcagc ggcggaacac agttcatatt gcctgccacc ggctcgacga
     9541 taatacaggc aatctcttgc gggtattgct caaacgcggc gcgtacagag gccagatcgt
     9601 tataagtaca ggttaaggta tgtttggcga aatctgccgg aacgcccggc gagtttggct
     9661 ggcctaacgt gagtgcgcca gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt
     9721 aacaaccttc aaatttaatg attttgtcgc gaccggtaaa accacgggcc aggcggatgg
     9781 cgctcatcgt cgcctcggtg ccggagttca ccatgcgcac catatccatg gtcgggacca
     9841 gttcggtcac cagttgcgcc attttcactt ccatttcggt cggtgcacca aagcttaaac
     9901 cacgctcggc ggcttcaatc acggcattgc ggatcgccgg atggttatgg cccagcacca
     9961 tcggccccca ggaaccgaca taatcgatat aggctttgcc atcaacatcg tacagataag
    10021 cgccgtccgc tttttcgata aacagtggag tgccgcccac gccagtaaag gcgcgaacag
    10081 gggagttcac accgccaggg atcagctcgc gcgctgcgct gtaaagattt tcagacttac
    10141 tcatggaggg ttcctgattc gtagaaaaag tgaatggctg ctattccatg ttattcataa
    10201 caagttaaat actcgtcaaa catcaggctg cttgtactgg tgcaatcctg atttcttaga
    10261 gtataaaagt tttgtgcatt tgaaacatta cgctttgcaa aggattttca tggaacgtgc
    10321 gagtaaaatg ccgtcatctt atttgtatga ccaataagtg atcattggat gaaaactgat
    10381 actccctctt tagaaacacc gcaggccgcg cgcctgcgac gcagacaact gattcgccaa
    10441 cttcttgagc gcgataaaac cccgttagcc attttgttta tggcggcagt cgtcggcacg
    10501 cttgtcgggc tggctgcggt tgcttttgac aaaggtgtcg cctggttgca gaaccaacgt
    10561 atgggggcgc tggtacatac tgctgataat tatccgctac tgttaaccgt cgcttttctc
    10621 tgttcggcgg tgctggcgat gttcggctac tttttggtgc gcaaatacgc gcccgaagca
    10681 gggggatcgg ggatcccgga aattgaaggg gcgctggaag atcaacgtcc tgttcgctgg
    10741 tggcgtgtat tgccggtgaa attctttggc gggctgggga cactcggcgg aggcatgggg
    10801 ctagggcgcg aaggaccaac cgtgcagatc ggcggcaata tcagtcgcat ggtgcttgat
    10861 attttccgcc tgaaaggtga tgaagctcgc catacgctgc tggcaaccgg tgctgctgcg
    10921 gggctggctg cggcctttaa cgcgccgctg gcgggtattt tgtttattat cgaagagatg
    10981 cgtccgcagt ttcgctatac gttaatttcg attaaagcgg tatttattgg cgtcattatg
    11041 tcgaccatta tgtaccggat ttttaatcat gaagttgcgt tgattgacgt cggtaaactt
    11101 tctgatgcgc cgcttaatac gctgtggctt tatctgatcc tcggtattat ttttggcatt
    11161 ttcggcccta tttttaataa atgggtgctg gggatgcagg atttgctgca ccgtgtgcac
    11221 ggcggcaata ttaccaaatg ggtactgatg gggggggcga ttggcggtct gtgtggattg
    11281 ctggggtttg tggcaccagc aacgtcgggc ggcggtttta acctgattcc tatcgctacc
    11341 gcggggaatt tcagcatggg aatgctggtg tttatcttcg tcgctcgggt cattaccacc
    11401 ttactctgct tctcttccgg cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt
    11461 actgtgctgg gaaccgcttt cggaatggtt gccgttgagc tgtttccgca atatcacctg
    11521 gaggcgggga cgtttgctat tgccggaatg ggggcattac tggcggcatc tattcgcgcg
    11581 ccgttaacgg ggatcattct ggttctggag atgaccgata actaccagct cattttgcca
    11641 atgattatta ccggtcttgg cgcaacacta ttagcgcaat ttaccggcgg gaaacctcta
    11701 tactcggcga ttcttgcgcg cacgctggca aaacaggaag ctgagcaact ggcgcgaagc
    11761 aaggccgcat cagccagcga gaatacttga acgaaatacc agggtattag ataatggcga
    11821 ttattattgg gttagaattt gcccgattgc cgatgtcgtt tggagcaaaa tatgagtgat
    11881 gacgtagcac tgccgctgga gtttaccgac gcagcagcca acaaagttaa aagcctgatc
    11941 gctgacgaag ataacccgaa tctgaaatta cgcgtgtata tcaccggtgg cggttgcagc
    12001 ggcttccagt atggtttcac ctttgatgat caggtgaacg aaggcgatat gaccatcgaa
    12061 aaacagggcg ttggcctggt ggttgatccg atgagcctgc aatatctggt cggcggttcc
    12121 gttgactaca ccgaaggact ggaaggttct cgtttcatcg tgaccaaccc gaacgcgaaa
    12181 agcacctgcg gttgcggttc ttcctttagc atctaatctg ttgttcatgt gccggataaa
    12241 ctatccggca catcttctct cttcaacgcc cattctcatc cagcgcaaac gtcggtagct
    12301 taagatgcca acgaatagcc gccagccgaa tcaatagcgt cacgaccatg cccatcatac
    12361 tggctgtttc cagtggtacg gaaaatgtgt aataagccgt agcgtggaca ataccgccga
    12421 taatacaggc agttgcgtag atttctgtac gtaaaatcat ggggatttcg cggaccagta
    12481 catcacgaat gatcccgccg ccaacgccag taatgacgcc catacaaacc gcgattaacg
    12541 gaccggcttc cgcattaaag gctttattca cgctaatgcc gacaaacacc gccagaccaa
    12601 cggcgtccag caccggcaac atccattttg gtaagcgtct tggctggcgc accagcacga
    12661 tggtcagcat gctggtgacc atggcaacga ccagatcggt gggatctttc acccaaaata
    12721 ccgggccgtg atccagcgcc atgtcgcgaa ttgttccgtc gcctactgcg gtaaccacgc
    12781 ccagtaccag aacaccaaaa gggtccatac gcaattttcc ggctaacaaa acgccgtaga
    12841 tggcaaatac cgctgtgccg actatatcca gccaatagac gagcattgtt caatccccac
    12901 tgagcacctg aaaaggtcag gcactaatct acctgtgaaa gcgcattaca gagctgttgt
    12961 gctgcgagga taatacgtgg gcttgcacgt tcaaaccagt cactcgtgag aggaataacg
    13021 ggaattttga gctgttcacc ccagtattgt ttgattttag gaatttggtc cggtccgcct
    13081 gtaatgacaa tagcctgtgg cgagcgtgct aacacctgtt cgcggctaac ttgcggccag
    13141 ggaacccggc tgtctttaaa gatgttttct ccgccacaaa cttcgagtac ctggttctga
    13201 atcgactctt ttccactggt aaatggcgga ttaatgccga attgcagaaa aacacgtttt
    13261 ttaggtttat cagcatattg cgctttcaat tgcacgtact gatccagcag ggattgcgcg
    13321 gcttgttcgg ccttgtctgg ttgcggactc cagggggcca gttgacgtaa cgcattggca
    13381 atttgttcaa tgcttgtcgc atcgacccac atcactttta ttcccagcga agccagctgg
    13441 tcaacctgcc gctcggcatt acctccacgc caggcaatca ccagatcggg tttcagcgcg
    13501 acaatgcgtt ccagattcat cccctgccag gtggaaacct gctcaatctt ttgcgcttgt
    13561 ggaggatagt cggaatagct gctgacccca accggcgtga tcccggcggc aaaggcaagt
    13621 tcagtgttgg cgggagaaag cgtgatgacg cgcggcgcgg cgttgagcca cagtggcgcg
    13681 agaaaagaca gggcgaccag cgccctgaac agtgatttag ccatgtgcca gtttctgcac
    13741 cagtgactca accatcaggc tggactgttt agcggcaaca gccaggaact catcgaagct
    13801 aagatgagac tgttgatcgg ccacgtcgga gatggcgcgt acgacaacaa acgggacgtt
    13861 gaaattgtgg cagacatggg cgattgccgt cgcttccatc tctacagcaa tggcctgtgg
    13921 gaagttgtgg cggattttcg ccagaccaac agaaccgttg atgaaagcgt cgccgctaac
    13981 aatcaggcca cgtacagcgt taagattcag ttcggcaatg caggcctcag cggcagcgat
    14041 cagtttatcg tcagctttaa agcctgccgg acagcctggt aactgaccgt attcataacc
    14101 aaatgccgtg acatccgcgt cgtgataacg cgcttcgtcc gagacaacga tatcgcccac
    14161 tttcagcgtt ggtgccaggc caccggcaga accggtgtta ataatcacat ctggcttgca
    14221 gtgttccaac agcagagtgg cacccagcgc cgcagcgact ttaccgatgc ccgatttcag
    14281 aagcgcaacc tcggttccat tcagttggcc ggtatagatt tcgcaaccgc cgagactgat
    14341 agtttgacgg ttttcgattt tgtcacgcag cagcgtaact tcttcttcca ttgcaccaat
    14401 gatgccgatt ttcatagatt tactcgcgat aagcccgatt tgaaggcata gtttaccatg
    14461 cgcttacggg gaagcgtatt tctcacgcgg gagaggacat ggcacagatt gatttccgaa
    14521 aaaaaataaa ctggcatcgt cgttaccgtt caccgcaggg cgttaaaacc gaacatgaga
    14581 tcctgcggat cttcgagagc gatcgcgggc gtatcatcaa ctctccggca attcgtcgtc
    14641 tgcaacaaaa gacccaggtt tttccactgg agcgcaatgc cgccgtgcgc acgcgtctta
    14701 cccactcgat ggaagtccag caggtgggac gctacatcgc caaagaaatt ttaagccgtc
    14761 tgaaagagct taaattgctg gaagcatacg gcctggatga actgaccggc ccctttgaaa
    14821 gcattgttga gatgtcatgc ctgatgcacg atatcggcaa tccgccgttt ggtcattttg
    14881 gcgaagcagc gataaatgac tggtttcgcc aacgtttgca cccggaagat gccgaaagcc
    14941 agcctctgac tgacgatcgc tgcagcgtgg cggcactacg tttacgggac ggggaagaac
    15001 cgcttaacga gctgcggcgc aagattcgtc aggacttatg tcattttgag gggaatgcac
    15061 aaggcattcg tctggtgcat acattgatgc ggatgaatct cacttgggca caggttggcg
    15121 gtattttaaa atatacccgt ccggcgtggt ggcgtggcga aacgcctgag acacatcact
    15181 atttaatgaa aaagccgggt tattatcttt ctgaagaagc ctatattgcc cgcttgcgta
    15241 aagaacttaa tttggcgctt tacagtcgtt ttccattaac gtggattatg gaagctgccg
    15301 acgacatctc ctattgtgtg gcagaccttg aagatgcggt agagaaaaga atatttaccg
    15361 ttgagcagct ttatcatcat ttgcacgaag cgtggggcca gcatgagaaa ggttcgctct
    15421 tttcgctggt ggttgaaaat gcctgggaaa aatcacgctc aaatagttta agccgcagta
    15481 cggaagatca gttttttatg tatttacggg taaacaccct aaataaactg gtaccctacg
    15541 cggcacaacg atttattgat aatctgcctg cgattttcgc cggaacgttt aatcatgcat
    15601 tattggaaga tgccagcgaa tgcagcgatc ttcttaagct atataaaaat gtcgctgtaa
    15661 aacatgtgtt tagccatccg gatgtcgagc agcttgaatt gcagggctat cgggtcatta
    15721 gcggattatt agagatttat cgtcctttat taagcctgtc gttatcagac tttactgaac
    15781 tggtagaaaa agaacgggtg aaacgtttcc ctattgagtc gcgcttattc cacaaactct
    15841 cgacgcgcca tcggctggcc tatgtcgagg ctgtcagtaa attaccgtca gattctcctg
    15901 agtttccgct atgggaatat tattaccgtt gccgcctgct gcaggattat atcagcggta
    15961 tgaccgacct ctatgcgtgg gatgaatacc gacgtctgat ggccgtagaa caataaccag
    16021 tcttttgtaa agacgaacaa taaattttta ccttttgcag aaactttagt tcggaacttc
    16081 gggctataaa acgaatctga agaacacagc aattttgcgt tatctgttaa tcgagactga
    16141 aatacatgaa aaaaaacaca ttagcactga gtgcactggc tctgagttta ggtttggcgt
    16201 tatctccgct ctctgcaacg gcggctgaga cttcttcagc aacgacagcc cagcagatgc
    16261 caagccttgc accgatgctc gaaaaggtga tgccttcagt ggtcagcatt aacgtagaag
    16321 gtagcacaac cgttaatacg ccgcgtatgc cgcgtaattt ccagcagttc ttcggtgatg
    16381 attctccgtt ctgccaggaa ggttctccgt tccagagctc tccgttctgc caggggggcc
    16441 agggcggtaa tggcggcggc cagcaacaga aattcatggc gctgggttcc ggcgtcatca
    16501 ttgatgccga taaaggctat gtcgtcacca acaaccacgt tgttgataac gcgacggtga
    16561 ttaaagtcca gctgagcgat ggtcgtaagt tcgacgcgaa gatggttggc aaagatccgc
    16621 gctctgatat cgcgctgatc caaatccaga acccgaaaaa actgaccgca attaagatgg
    16681 cggattctga tgcactgcgc gtgggtgatt acaccgtagc gattggtaac ccgtttggtc
    16741 tgggcgagac ggtaacttcc gggattgtct ctgcgctggg gcgtagcggc ctgaatgctg
    16801 aaaactacga aaacttcatc cagaccgatg cagcgatcaa ccgtggtaac tccggtggtg
    16861 cgctggttaa cctgaacggc gaactgatcg gtatcaacac cgcgatcctc gcaccggacg
    16921 gcggcaacat cggtatcggt tttgctatcc cgagtaacat ggtgaaaaac ctgacctcgc
    16981 agatggtgga atacggccag gtgaaacgcg gtgagctggg tattatgggg actgagctga
    17041 attccgaact ggcgaaagcg atgaaagttg acgcccagcg cggtgctttc gtaagccagg
    17101 ttctgcctaa ttcctccgct gcaaaagcgg gcattaaagc gggtgatgtg atcacctcac
    17161 tgaacggtaa gccgatcagc agctttgccg cactgcgtgc tcaggtgggt actatgccgg
    17221 tgggcagcaa actgaccctg ggcttactgc gcgacggtaa gcaggttaac gtgaacctgg
    17281 aactgcagca gagcagccag aatcaggttg attccagctc catcttcaac ggcattgaag
    17341 gcgctgagat gagcaacaaa ggcaaagatc agggcgtggt agtgaacaac gtgaagacgg
    17401 gcactccggc tgcgcagatc ggcctgaaga aaggtgatgt gattattggt gcgaaccagc
    17461 aggcagtgaa aaacatcgct gaactgcgta aagttctcga cagcaaaccg tctgtgctgg
    17521 cactgaacat tcagcgcggc gacagcacca tctacctgtt aatgcagtaa tctccctcaa
    17581 ccccttcctg aatacgggaa ggggttctcc ttacaatctg tgaacttcgc cacaactcca
    17641 tacatcttca tcatcctttg ggcatttgca caatgccgta cgttacgtac ttccttatgc
    17701 taagccgtgc ataacggagg acttatggct ggctggcatc ttgataccaa aatggcgcag
    17761 gatatcgtgg cacgtaccat gcgcatcatc gataccaata tcaacgtaat ggatgcccgc
    17821 gggcgaatta tcggcagcgg cgatcgtgag cgtattggtg aattgcacga aggtgcattg
    17881 ctggtacttt cacagggacg agtcgtcgat atcgatgacg cggtagcacg tcatctgcac
    17941 ggtgtgcggc aggggattaa tctaccgtta cggctggaag gtgaaattgt cggcgtaatt
    18001 ggcctgacag gtgaaccaga gaatctgcgt aaatatggcg aactggtctg catgacggct
    18061 gaaatgatgc tggaacagtc gcggttgatg cacttgttgg cgcaggatag ccgtttgcgg
    18121 gaagaactgg tgatgaacct gattcaggca gaggagaata ctcccgcact tctgaatggg
    18181 cgcaacggct ggggatcgat ctcaatcaac cgcgagtggt ggctattgtt gaggtcgaca
    18241 gcggtcagct tggcgtggac agcgcaatgg cggagttaca acaactgcaa aacgcgctga
    18301 ctacgcccga gcgtaataat ctggtggcga ttgtctcgct aaccgaaatg gtggtgttga
    18361 aaccggcgtt gaactctttt gggcgctggg atgcagaaga tcatcgtaag cgagttgaac
    18421 aactgattac ccgcatgaaa gagtacggcc agctgcgttt tcgcgtttca ctgggcaact
    18481 attttaccgg tcctggcagt attgcccgat cctatcgtac ggcgaaaacg acgatggtgg
    18541 tgggtaaaca gcggatgcca gaaagtcgct gctattttta tcaggatctg atgttacctg
    18601 tgttactcga cagtttgcgt ggcgactggc aggccaacga actggcgcga ccgctggcgc
    18661 ggctgaaagc gatggacaat aacggcttgc tgcgacgaac gctggcggcg tggtttcgcc
    18721 acaatgtgca accgctggca acgtcaaagg cgttgtttat tcatcgtaat accctggagt
    18781 atcggcttaa tcgtatatcg gaactgaccg ggcttgattt gggcaatttt gatgacaggt
    18841 tgctgctgta tgtggcgtta caactggatg aagagcggta gattatgcgt taaggttgag
    18901 gcggcgctgg ctcattcccc tcagcccgaa ggggagaggg gaatgcccga gttaaccgtc
    18961 aattcgggat tatttacgcg ttagtttttc cagatcggct tcaatctcgc tgatcttatt
    19021 ggttacgact gactccaggt gacgtaagtc gtcgaggatc ttacgcttaa gatcaacttc
    19081 gctgcggtca cgctgacaga tttgatcaag ctcatcaatg atataccgta gattcgggct
    19141 gatttcctgg acttctttat aaccctgacc cacaccatca gcgacgaccg ttttacgctg
    19201 acgcggatat ttaaacttaa cgctcttggc gaaaaactcg cctttgtctt tctggaaata
    19261 gattttcaga atatcgttgt tggcttcctg ccggaggctg taacgatcaa tttcttcagg
    19321 attggtaata cccagacttt tcagattgtc gtacatagcg ttacctcaaa atgagtcagt
    19381 aaattgtgct tattttagca tttggcttgg cccgcccggc tcgactgtga tcgcagaaag
    19441 cctttttctt ccagacttct atcatggcgt aaaaatcaaa aaattacctg ctttattgtg
    19501 gcgatattcc atgggatgga ttagcacatt tatcttcgtt ttgcgaatta catgacagtc
    19561 cgaaacaaga tgaaaaagta tttaaaaaag ggggaggaag aatgagtagg acgaattatt
    19621 ctgagccaga aagcatacgg gcagcatcat gctgcccgta tatgtactct taatcgatgg
    19681 tacgcagcag ttcgttaatg ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga
    19741 taaccgcaca gtagaggctg tatttgccat cttttgacgg cagattacct gaaacaacca
    19801 cagaccccgc cggaacgcga ccgtagtgga tttcgccggt ttcacggtcg taaatacggg
    19861 tgctctgacc aatgtatacg cccatagaga tgacggaacc ttcttcgaca atcacccctt
    19921 caaccacttc agagcgtgcg ccaatgaagc agttatcttc aataatggtc gggttagcct
    19981 gcagcggctc cagcacgccg ccgatgccca cgccaccgga aaggtggacg tttttaccaa
    20041 tctgcgcaca agaaccgacg gtcgcccagg tatcaaccat ggtgccttca tcaacatatg
    20101 cgccgatgtt aacgtaagac ggcatcagca cggtgttacg ggcaataaac gcaccctgac
    20161 gtaccgccgc tggtggcaca acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt
    20221 cggcgaattt catcggcact ttgtcgaagt agcggctttc tgccccttcg atcacctgat
    20281 tatcattaat acggaaagag agcagcaccg cttttttcaa ccactgatgc gtcacccact
    20341 gaccgtcaat tttttccgct acacgcagtg cgccggaatc cagcagggcg atcacctgat
    20401 ttaccgcttc gcgggtaacg gtgtctgcat tggctggcgt gatctcggca cggcgttcaa
    20461 aagcggtttc aataatgttc tgtaactgct gcattgttaa actcttttca tatcagtaaa
    20521 cacatcaccc tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc acttcctgct
    20581 gcaactcgtt attaagcgca cgccggtcag cggtggcaat tatgaataaa tcttctactc
    20641 gctcgccaat ggttgtaatt cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt
    20701 tcccgactcg cgccagcagt ccaggttggt cgagggcgat cagttcgagg aacgatttgc
    20761 ggtcggtatg ggtcggcaaa aacgttactt cggtttcaac agtaaaatgg cgtaatttgg
    20821 cgggttggcg acggggctgc ggtggctgcc agctactttg cgtcagcacc tgctccagac
    20881 caaaccgaat aacctcatgg cgatctgcgg acagcgggct gccatcgggt tccagcacga
    20941 taaaggtatc catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta
    21001 aattgcggcg gtctaattcg gcacagacgg cggcaaacag ataggggcgg tccgggctcc
    21061 agataaaaat ctcggtgcct ccacgcgtag cctgcgggct aagcaatacc agcggtttgc
    21121 ttaaatcatg ctgtaataaa tggcgggcat gccaggccag ttgatttggg ctatggcgga
    21181 caaaatagtt agcacgacag cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat
    21241 ccatgcgcag tagtgccagt gcctggagtt ggtgatggcg aacccgttcg cgcatatccg
    21301 gcgtgttttg cattccgcgt cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca
    21361 gactttgctt ccagctattc cacagtgttt cgttggtggc gcaaatgtca gccactgtca
    21421 ggcataccag atagcgcaga cgattttccg tttgaacttc ttcggcaaac tgcttgatga
    21481 cttcagggtc ctgaatatcg cggcgttgtg ctgtcaccga catcagcaag tgctggcgaa
    21541 ccagccaggc aaccagctgt gtttcgcgtg agttcagccc gtggagttct gcaaaatgca
    21601 ctacatcctg agcaccgaga atggagtggt cgccgccgcg tcctttggcg atatcgtgaa
    21661 acagcgcggc gatgaaaatc agctcagttg acggcaggcg cggccagacg tccacacaca
    21721 acggatggcg ctggcgcgtt tcttcactgg caaaactctc cagtttcagc atcacgcgga
    21781 tagtatgttc atccaccgtg taggcgtgga acagatcaaa ctgcatctgc ccgacgatat
    21841 gcgaccattg cggcatatac gcgccgagca cgctatggcg atgcattggc aatagtccgc
    21901 gccgcaccgc tccggggtga cgcagaatgc tcaaaaacag ttttcgtgct tgcggaatat
    21961 tacacagcgg ttgttgcaga tggcgacggg catggcgtaa ctggcgcagc gtggtggagt
    22021 aaatgccggt gatcgcactg ttgcgcacca tggtgtagaa catacgcaag atggcttccg
    22081 gctggcgcat aaatagtgtt tcatcacgca ggtcgattag cgtaccgcgt agctgaaact
    22141 catcgtcgag tggacgtggt ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca
    22201 gttgcagcag catctggttg agttcactga cgcggcgtgt aacgcggaag taatccttca
    22261 tcatccgctc gaccggttcg ttgccttcac cactgtaatt cagacgctga gcgacgctaa
    22321 gttggcgatc gaataacaga cgattatcgt aacggctgac gaccagatgc agggcaaagc
    22381 gaatacgcca cagtatatgc agacattcgt ttaattccgc ccgctccgct gaggttaaga
    22441 agccaaaccc gaccatttca tccagcgatg ttgcgccaaa atgacggcgg gccacccatt
    22501 gcagagtgtg aatatcgcgc aagccgccag ggctactttt gatgtctggt tcaaggttgt
    22561 agctggtgcc atggtaacgc tgatggcgct ggttctgttc ttcaactttc gctgcgtaga
    22621 acttgtcgga aggccagaat ccttcgctaa aaatatgttt ttgcagttcg aggaagagcg
    22681 caacatcgcc aattaataag cgggattcga ttaaattggt ggcgacggtt aaatccgata
    22741 acccttccaa catgcactct tcaagcgtgc gcacactatg accgacttcc agctttacat
    22801 cccagagcag cgttaacagc tcgcccactt tttgcgcctg atcgtccggg agctttttac
    22861 ggcttaaaat cagtaaatcg atgtcagaaa gtggatgcag ctcgccacga ccgtagccac
    22921 cgacggcaac caatgccagg tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt
    22981 gcaggagctg gtctatgaac tcggtacgtg cctcaatcaa ctgttctgca gagatcccat
    23041 tgtcaaaggc atcacccagc caacgctgga aagtatcgat atgggctttt atcccacaga
    23101 cggttaattc atcacggggc caggcgcatg gattttgcgg ttgaccgggc agggtggaga
    23161 gagcggtgtt tgcgtactgt tctggaaggg tattcattgt gcgccaccca taagattaaa
    23221 ttatcgcatt aaaaaagccg gcattcgccg gcttcatctt attcgtcgtg cgagattatc
    23281 gccgggatgg tgtcatcctt gcgtagcgtc aaaatttcgc agccgttatc agtcaccaca
    23341 atagtatgct catattgtgc agacaagctg cgatctttgg tttttaccgt ccagccatct
    23401 ttcatggtgc ggatctcttt tttacccgcg ttgaccattg gctcaatggt gaacgtcatc
    23461 cccggtttca gcacgacgtt ggtttcacgg gagtcatagt gcagcacctg cggttcttca
    23521 tggaagccgc gaccaatacc gtgtccgcaa tattcacgaa cgacggagaa gccttctgct
    23581 tcgacaaatt tctgaatcgc cgcaccgatt tcgcgcagat taatgcctgg ttttaccatg
    23641 cgtagcgcca ggtacaggct ttcttgcgtg atgcggcaca ggcgttcgcc catgatggtc
    23701 ggcttaccga cgataaacat tttcgaggta tcgccgtgga aaccatcttt gattacggtg
    23761 acatcaatgt taacgatatc gccatctttc agcagcttag catcgtccgg gataccgtgg
    23821 cacaccactt cattaataga gatgcaaacg gatttcggat agccgtgata gccgaggcag
    23881 gcagaaaccg cgtgttgttc attaacaatg taatcattac agatgcgatc cagctcgccg
    23941 gtgctgacgc ccggtttaac atacggttcg atcatctcca gcacttcggc agccagtcgg
    24001 ccagcgacgc gcattttttc gatatcttct ggggtcttga ttgagatagc cattaattct
    24061 gtccatcagc gtcggtgata ccgacaatat atatgtaagt gccgtcaatg gtatcacacc
    24121 cgggcaaatt gagaatcatt ctgaatttcg ccaaacgtgc cactgaaggt tttctataat
    24181 agaaaattcg acgtctgatg ctgtacacag cgccaacaat tattggtgtc cacgacgtat
    24241 ttgtggtata aagcgcgccg gacttccgat ccatttcgta tacacagact ggacggaagc
    24301 gacaatctca ctttgtgtaa caacacacac gtatcggcac atattccggg gtgccctttg
    24361 gggtcggtaa tatgggatac gtggaggcat aaccccaact tttatataga ggttttaatc
    24421 atggcaactg tttccatgcg cgacatgctc aaggctggtg ttcacttcgg tcaccagacc
    24481 cgttactgga acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa agttcacatc
    24541 atcaaccttg agaaaactgt accgatgttc aacgaagctc tggctgaact gaacaagatt
    24601 gtttctcgca aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg
    24661 aaagacgctg ctctgagctg cgaccagttc ttcgtgaacc atcgctggct gggcggtatg
    24721 ctgactaact ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag
    24781 tctcaggacg gtactttcga caagctgacc aagaaagaag cgctgatgcg cactcgtgag
    24841 ctggagaaac tggaaaacag cctgggcggt atcaaagaca tgggcggtct gccggacgct
    24901 ctgtttgtaa tcgatgctga ccacgaacac attgctatca aagaagcaaa caacttgggt
    24961 attccggtat ttgctatcgt tgataccaac tctgatccgg acggtgttga cttcgttatc
    25021 ccgggtaacg acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc
    25081 gtacgtgaag gccgttctca ggatctggct tcccaggcgg aagaaagctt cgtagaagct
    25141 gagtaataag gcttgataac tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc
    25201 gagaattccc gggagcttac atcagtaagt gaccgggatg agcgagcgaa gacaacgcat
    25261 ctgcggcgcg aaatatgaag ggggagagcc cttatagacc aggtagtaca cgtttggtta
    25321 gggggcctgc atatggcccc ctttttcact tttatatctg tgcggtttaa tgccgggcag
    25381 atcacatctc cgaggatttt agaatggctg aaattaccgc atccctggta aaagagctgc
    25441 gtgagcgtac tggcgcaggc atgatggatt gcaaaaaagc actgactgaa gctaacggcg
    25501 acatcgagct ggcaatcgaa aacatgcgta agtccggtgc tattaaagca gcgaaaaaag
    25561 caggcaacgt tgctgctgac ggcgtgatca aaaccaaaat cgacggcaac tacggcatca
    25621 ttctggaagt taactgccag actgacttcg ttgcaaaaga cgctggtttc caggcgttcg
    25681 cagacaaagt tctggacgca gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag
    25741 cacagttcga agaagaacgt gttgcgctgg tagcgaaaat tggtgaaaac atcaacattc
    25801 gccgcgttgc tgcgctggaa ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg
    25861 gcgttctggt tgctgctaaa ggcgctgacg aagagctggt taaacacatc gctatgcacg
    25921 ttgctgcaag caagccagaa ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa
    25981 aagaatacca ggtacagctg gatatcgcga tgcagtctgg taagccgaaa gaaatcgcag
    26041 agaaaatggt tgaaggccgc atgaagaaat tcaccggcga agtttctctg accggtcagc
    26101 cgttcgttat ggaaccaagc aaaactgttg gtcagctgct gaaagagcat aacgctgaag
    26161 tgactggctt catccgtttc gaagtgggtg aaggcatcga gaaagttgag actgactttg
    26221 cagcagaagt tgctgcgatg tccaagcagt cttaattatc aaaaaggagc cgcctgaggg
    26281 cggcttcttt ttgtgcccat cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa
    26341 gcgacgtaca atgtcgctag tattaattca tttcaatcgt tgacagtctc aggaaagaaa
    26401 catggctacc aatgcaaaac ccgtctataa acgcattctg cttaagttga gtggcgaagc
    26461 tctgcagggc actgaaggct tcggtattga tgcaagcata ctggatcgta tggctcagga
    26521 aatcaaagaa ctggttgaac tgggtattca ggttggtgtg gtgattggtg ggggtaacct
    26581 gttccgtggc gctggtctgg cgaaagcggg tatgaaccgc gttgtgggcg accacatggg
    26641 gatgctggcg accgtaatga acggcttggc aatgcgtgat gcactgcacc gcgcctatgt
    26701 gaacgctcgt ctgatgtccg ctattccatt gaatggcgtg tgcgacagct acagctgggc
    26761 agaagctatc agcctgttgc gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa
    26821 cccgttcttt accaccgact cagcagcttg cctgcgtggt atcgaaattg aagccgatgt
    26881 ggtgctgaaa gcaaccaaag ttgacggcgt gtttaccgct gatccggcga aagatccaac
    26941 cgcaaccatg tacgagcaac tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat
    27001 ggacctggcg gccttcacgc tggctcgtga ccataaatta ccgattcgtg ttttcaatat
    27061 gaacaaaccg ggtgcgctgc gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac
    27121 ggaataattc ccgtgatgga taaataaggg taagattccg cgtaagtatc gcgggggcgt
    27181 aagtctggtt ataaggcgtt attgttgcag gcagtttggt cacggccagc gcgcagcaac
    27241 cggagcgtac aaaagtacgt gaggatggcg agcactgctc ggggccaaaa tggcaaataa
    27301 aatagcctaa taatccagac gattacccgt aatatgttta atcagggcta tacttagcac
    27361 acttccactg tgtgtgactg tctggtctga ctgagacaag ttttcaagga ttcgtaacgt
    27421 gattagcgat atcagaaaag atgctgaagt acgcatggac aaatgcgtag aagcgttcaa
    27481 aacccaaatc agcaaaatac gcacgggtcg tgcttctccc agcctgctgg atggcattgt
    27541 cgtggaatat tacggcacgc cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga
    27601 ttcccgtact ctgaaaatca acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc
    27661 gattatggcc tccgatctcg gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc
    27721 gctgccgccg ctgacggaag aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc
    27781 agaacaagcg cgtgttgcag tacgtaatgt gcgtcgtgac gcgaacgaca aagtgaaagc
    27841 actgttgaaa gataaagaga tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca
    27901 gaaactgact gatgctgcaa tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga
    27961 actgatgcag ttctgatttc ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc
    28021 ggctggcggc gttttgcttt ttattctgtc tcaactctgg atgtttcatg aagcaactca
    28081 ccattctggg ctcgaccggc tcgattggtt gcagcacgct ggacgtggtg cgccataatc
    28141 ccgaacactt ccgcgtagtt gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac
    28201 agtgcctgga attctctccc cgctatgccg taatggacga tgaagcgagt gcgaaacttc
    28261 ttaaaacgat gctacagcaa caggggagcc gcaccgaagt cttaagtggg caacaagccg
    28321 cttgcgatat ggcagcgctt gaggaggttg atcaggtgat ggcagccatt gttggtgctg
    28381 ctgggctgtt acctacgctt gctgcgatcc gcgcgggtaa aaccattttg ctggccaata
    28441 aagaatcact ggttacctgc ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc
    28501 aattgttacc ggtcgatagc gaacataacg ctatttttca gagtttaccg caacctatcc
    28561 agcataatct gggatacgct gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg
    28621 ggtctggtgg ccctttccgt gagacgccat tgcgcgattt ggcaacaatg acgcccgatc
    28681 aagcttgccg tcatccgaac tggtcgatgg gacgtaaaat ttctgtcgat tcggctacca
    28741 tgatgaacaa aggtctggaa tacattgaag cgcgttggct gtttaacgcc agcgccagcc
    28801 agatggaagt gctgattcac ccgcagtcag tgattcactc aatggtgcgc tatcaggacg
    28861 gcagtgttct ggcgcagctg ggggaaccgg atatgcgtac gccaattgcc cacaccatgg
    28921 catggccgaa tcgcgtgaac tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt
    28981 tgacatttgc cgcaccggat tatgagcgtt atccatgcct gaaactggcg atggaggcgt
    29041 tcgaacaagg ccaggcagcg acgacagcat tgaatgccgc aaacgaaatc accgttgctg
    29101 cttttcttgc gcaacaaatc cgctttacgg atatcgctgc gttgaattta tccgtactgg
    29161 aaaaaatgga tatgcgcgaa ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg
    29221 cgagtgaagt cgccagaaaa gaggtgatgc gtctcgcaag ctgaggataa tccggctaca
    29281 gagagtcgcg ctatttgtta gcgtagggct tcagtgatat agtctgcgcc atctgatcgt
    29341 aagtagttgg ctttataagg tcaggtatgc cgtggtttta cacggctttt ttttgtatag
    29401 gcttcagtat tcctgagtac cgtaaaccct gtcagggaat aaaaaacgcg tgatgttgtc
    29461 tgctactcaa ccacttagcg aaaaattgcc agcgcatggc tgccgtcatg ttgcgatcat
    29521 tatggacggc aatggtcgct gggcaaaaaa gcaagggaag attcgtgcct ttgggcataa
    29581 agccggggca aaatccgtcc gccgggctgt ctcttttgcg gccaacaacg gtattgaggc
    29641 gttaacgctg tatgccttta gtagtgaaaa ctggaaccga ccagcgcagg aagtcagtgc
    29701 gttaatggaa ctgtttgtgt gggcgctcga tagcgaagta aaaagtctgc accgacataa
    29761 cgtgcgtctg cgtattattg gcgataccag tcgttttaac tcgcgtttgc aagaacgtat
    29821 tcgtaaatct gaagcgctaa cagccggaaa taccggtctg acgctgaata ttgcggcgaa
    29881 ctacggtgga cgttgggata tagtccaggg agtcaggcaa ctggctgaaa aggtgcagca
    29941 aggaaacctg caaccagatc agatagatga agagatgtta aaccagcatg tctgtatgca
    30001 tgaactggcc cctgtagatt tagtaattag gactggggga gagcatcgca ttagtaactt
    30061 tttgctttgg caaattgcct atgccgaact ttactttaca gatgttctct ggcccgattt
    30121 cgatgaacaa gactttgaag gggcgttaaa tgcctttgct aatcgagagc gtcgtttcgg
    30181 cggcaccgag cccggtgatg aaacagcctg atgggggtcg cttttgctga agtatcgcct
    30241 gatatctgct tttgtgttaa tacccgtcgt catcgcggcg ttgtttctgt tgccgccggt
    30301 ggggttcgcc attgtaacgc tggtggtctg catgctggca gcgtgggaat ggggacagct
    30361 tagcggtttt accactcgtt cgcagcgagt atggttggcg gtgttatgcg ggttattgtt
    30421 ggcgctgatg ctttttctgt tgccggaata tcaccgaaat attcatcaac cgctggttga
    30481 aatctcactt tgggcttcgt tgggttggtg gattgtcgcg ctattgctgg tgctgtttta
    30541 cccaggttcc gcagcaatct ggcgtaactc taaaacattg cgccttattt ttggcgtgct
    30601 aaccattgtt cccttcttct ggggcatgct ggcgttacgg gcctggcact atgacgagaa
    30661 tcattacagt ggcgcaatat ggctgctcta tgtcatgatc ctggtatggg gcgctgactc
    30721 cggcgcatat atgtttggca aattgtttgg taaacataag ctggcaccga aggtttctcc
    30781 gggtaaaacc tggcaaggct ttataggtgg actcgctact gcagcggtaa tctcatgggg
    30841 ttatggcatg tgggcgaatc tcgacgtcgc tcccgtcacc ttactcattt gctctattgt
    30901 cgcagcgtta gcctcagtgc tcggcgatct gaccgagagt atgtttaagc gtgaagcagg
    30961 aattaaggac agcggtcatt taattccagg acacggtggt attttagatc gtattgatag
    31021 cctgacggct gcggtaccgg tctttgcttg cttgttgtta ctggtattca ggacgcttta
    31081 acggaaggta atatgctgag ttttctctgg gatttggctt cgttcatcgt tgcactgggt
    31141 gtacttatca ccgtgcatga atttggtcat ttctgggttg cccggcgttg tggtgttcgc
    31201 gttgagcgtt tctcaatagg gtttggtaag gcgctctggc ggcgaactga taagctcggc
    31261 accgaatatg ttatcgccct gatcccgttg ggcggttatg tcaaaatgct ggatgagcgc
    31321 gcagaaccgg tcgttccgga actccgccac catgccttca ataataaatc tgtcggccaa
    31381 cgagcggcga ttattgccgc aggtccggtt gcaaacttca tttttgctat ctttgcctac
    31441 tggctggttt ttattattgg tgtgcctggc gtacgtccgg tggttggcga aatagcagcc
    31501 aattcgatag ctgcggaagc acaaattgca ccaggtacgg aactaaaagc cgtagatggt
    31561 atcgaaacgc ctgattggga tgccgtgcgt ttgcagttgg tcgataaaat tggcgatgaa
    31621 agcaccacca ttacagtagc gccatttgac agcgaccaac ggcgggatgt aaagctcgat
    31681 ttacgtcact gggcgtttga gcctgataaa gaagatccgg tatcttcgct ggggattcgt
    31741 cctcgtgggc cgcaaattga acctgtactg gaaaatgtgc agccaaactc ggcggcaagc
    31801 aaggcaggtt tgcaagcagg cgacaggatc gttaaagtcg atggtcagcc cttaacgcag
    31861 tgggtgacct ttgtgatgct tgtccgggat aacccgggta aatccttagc gttagaaatc
    31921 gaaaggcagg ggagtccctt gtctttgaca ttaatcccgg agagtaaacc gggtaatggt
    31981 aaagcgattg gttttgtcgg tattgagccg aaagtcattc ctttgccaga tgagtataaa
    32041 gttgtacgcc agtatgggcc gttcaacgcc atcgtcgaag ccacggacaa aacgtggcag
    32101 ctgatgaagc tgacggtcag tatgctggga aaattgatca ccggtgatgt gaaactgaac
    32161 aacctcagtg ggccgatctc tatcgccaag ggggctggga tgacagcgga actcggggta
    32221 gtttattacc tgccgtttct tgcgcttatt agcgtgaact tagggataat taacctgttt
    32281 ccgttgcccg tacttgacgg ggggcatctg ctgttccttg cgatcgaaaa gatcaagggc
    32341 ggaccggtat ccgagcgggt tcaagacttt tgttatcgca ttggctcgat tctgctggtg
    32401 ctgttaatgg ggcttgcact tttcaatgat ttctctcggt tatgagagtt agttaggaag
    32461 aacgcataat aacgatggcg atgaaaaagt tgctcatagc gtcgctgctg tttagcagcg
    32521 ccaccgtata cggtgctgaa gggttcgtag tgaaagatat tcatttcgaa ggccttcagc
    32581 gtgtcgccgt tggtgcggcc ctcctcagta tgccggtgcg tacaggcgac acggttaatg
    32641 atgaagatat cagtaatacc attcgcgctc tgtttgctac cggcaacttt gaggatgttc
    32701 gcgtccttcg tgatggtgat acccttctgg ttcaggtaaa agaacgtccg accattgcca
    32761 gcattacttt ctccggtaac aaatcggtga aagatgacat gctgaagcaa aacctcgagg
    32821 cttctggtgt gcgtgtgggc gaatccctcg atcgcaccac cattgccgat atcgagaaag
    32881 gtctggaaga cttctactac agcgtcggta aatatagcgc cagcgttaaa gctgtcgtga
    32941 ccccgctgcc gcgcaaccgt gttgacctaa aactggtgtt ccaggaaggt gtgtcagctg
    33001 aaatccagca aattaacatt gttggtaacc atgctttcac caccgacgaa ctgatctctc
    33061 atttccaact gcgtgacgaa gtgccgtggt ggaatgtggt aggcgatcgt aaataccaga
    33121 aacagaaact ggcgggcgat cttgaaaccc tgcgcagcta ctatctggac cgcggttatg
    33181 cccgtttcaa catcgactct acccaggtca gtctgacgcc agataaaaaa ggtatttacg
    33241 tcacggtgaa catcaccgaa ggcgatcagt acaagctttc tggcgttgaa gtgagcggca
    33301 accttgccgg gcactccgct gaaattgagc aactgactaa gatcgagccg ggcgagctgt
    33361 ataacggcac taaagtgacc aaaatggaag atgacattaa aaagcttctc ggtcgctatg
    33421 gttatgccta tccgcgcgta cagtcgatgc ctgaaattaa cgatgccgac aaaaccgtta
    33481 aattacgcgt gaacgttgat gcgggtaacc gtttctacgt gcgtaagatc cgttttgaag
    33541 gtaacgatac ctcgaaagat gccgtcctgc gtcgcgaaat gcgtcagatg gaaggtgcat
    33601 ggttggggag cgatctggtc gatcagggta aggagcgtct gaatcgtctg ggcttctttg
    33661 aaactgtcga taccgatacc caacgtgttc cgggtagccc ggaccaggtt gatgtagtct
    33721 acaaggtgaa agaacgtaac actggtagct tcaacttcgg gattggttac ggtactgaaa
    33781 gtggcgtgag cttccaggct ggcgtgcaac aggataactg gttaggtaca ggttatgctg
    33841 ttggtatcaa cgggaccaaa aacgattacc agacctatgc tgaactgtcg gtaaccaacc
    33901 cgtacttcac cgtagatggc gtaagcctcg gtggtcgtct cttctataat gacttccagg
    33961 cagatgacgc cgacctgtcc gactatacca acaagagtta tggtacagac gtgacgttgg
    34021 gcttcccgat taacgaatat aactcgctgc gtgcaggtct gggttatgta cataactccc
    34081 tgtccaacat gcagcctcag gttgcgatgt ggcgttatct gtactctatg ggtgaacatc
    34141 cgagcacctc tgatcaggat aacagcttca aaacggacga cttcacgttc aactatggtt
    34201 ggacctataa caagcttgac cgtggttact tcccgacaga tggttcacgt gtcaacctga
    34261 ccggtaaagt gaccattcct ggatcggata acgaatacta caaagtgacg ttagacacgg
    34321 cgacttatgt gccgatcgat gacgatcaca aatgggttgt tctgggtcgt acccgctggg
    34381 gttatggtga tggtttaggc ggcaaagaga tgccgttcta cgagaacttc tatgccggtg
    34441 gttccagcac cgtgcgtggc ttccagtcca ataccattgg tccgaaagca gtttacttcc
    34501 cgcatcaggc cagtaattat gatccggact atgattacga atgtgcgact caggacggcg
    34561 cgaaagacct gtgtaaatcg gatgatgctg taggcggtaa cgccatggcg gttgccagcc
    34621 tcgagttcat caccccgacg ccgtttatta gcgataagta tgctaactcg gttcgtactt
    34681 cattcttctg ggatatgggt accgtttggg atacaaactg ggattccagc caatattctg
    34741 gatatccgga ctatagtgat ccaagcaata tccgtatgtc tgcgggtatc gcattacaat
    34801 ggatgtcccc attggggccg ttggtgttct cctacgccca gccgttcaaa aagtacgatg
    34861 gagacaaggc agaacagttc cagtttaaca tcggtaaaac ctggtaagtg ttctccacaa
    34921 aggaatgtag tggtagtgta gcgatgactt taggcgatca atataagatc gccgggccac
    34981 gcaaagaact gcaccctccg gtgcaaatgg gatggtaagg agtttattgt gaaaaagtgg
    35041 ttattagctg caggtcttgg tttagcactg gcaacttctg ctcaggcggc tgacaaaatt
    35101 gcaatcgtca atatgggcag cctgttccag caggtagcgc agaaaaccgg tgtttctaac
    35161 acgctggaaa atgagttcaa aggccgtgcc agcgaactgc agcgtatgga aaccgatctg
    35221 caggctaaaa tgaaaaaact gcagtccatg aaagcgggca gcgatcgcac caaactggaa
    35281 aaagacgtga tggctcagcg ccagactttt gctcagaaag cgcaggcttt tgagcaggat
    35341 cgcgcacgtc gttccaacga agaacgcggc aaactggtta ctcgtatcca gactgctgtg
    35401 aaatccgttg ccaacagcca ggatatcgat ctggttgttg atgcaaacgc cgttgcttac
    35461 aacagcagcg atgtaaaaga catcactgcc gacgtactga aacaggttaa ataagtaatg
    35521 ccttcaattc gactggctga tttagcgcag cagttggatg cagaactaca cggtgatggc
    35581 gatatcgtca tcaccggcgt tgcgtccatg caatctgcac aaacaggtca cattacgttc
    35641 atggttaacc caaaataccg tgagcattta ggcttgtgcc aggcgtccgc ggttgtcatg
    35701 acccaggacg atcttccttt cgcgaaaagt gccgcgctgg tagtgaagaa tccctacctg
    35761 acttacgcgc gcatggcgca aattttagat accacgccgc agcccgcgca gaacattgca
    35821 cccagtgcgg tgatcgacgc gacggcgaag ctgggtaaca acgtatcgat tggcgctaac
    35881 gcggtgattg agtccggcgt tgaactgggc gataacgtga ttatcggtgc cggttgcttc
    35941 gtaggtaaaa acagcaaaat cggtgcaggt tcgcgtctct gggcgaacgt aaccatttac
    36001 catgagatcc agatcggtca gaattgcctg atccagtccg gaacagtggt aggcgcagac
    36061 ggctttggtt atgccaacga tcgtggtaac tgggtgaaga tcccacagat tggtcgcgta
    36121 attattggcg atcgcgtgga gatcggtgcc tgcacaacca tcgatcgcgg cgcgctggat
    36181 gacactgtta ttggcaatgg cgtgatcatt gataaccagt gccagattgc acataacgtc
    36241 gtgattggcg acaatacggc ggttgccggt ggcgtcatta tggcgggcag cctgaaaatt
    36301 ggtcgttact gcatgatcgg cggagccagc gtaatcaacg ggcatatgga aatatgcgac
    36361 aaagtgacgg ttacgggcat gggtatggtg atgcgtccca tcactgaacc aggcgtctat
    36421 tcctcaggca ttccgctgca acccaacaaa gtctggcgca aaaccgctgc actggtgatg
    36481 aacattgatg acatgagcaa gcgtctgaaa tcgcttgagc gcaaggttaa tcaacaagac
    36541 taacgttcca tcttttgttc gccaaacttt acggcctgtc tcattcttac gattgcggca
    36601 ggccgtgtta ttattgtcgt ttcttatatt ttgacaggaa gagtatcttg actactaaca
    36661 ctcatactct gcagattgaa gagattttag aacttctgcc gcaccgtttc ccgttcttac
    36721 tggtggatcg cgtgctggat tttgaagaag gtcgttttct gcgcgcagta aaaaatgtct
    36781 ctgtcaatga gccattcttc cagggccatt tccctggaaa accgattttc ccgggtgtgc
    36841 tgattctgga agcaatggca caggcaacag gtattctggc gtttaaaagc gtaggaaaac
    36901 tggaaccggg tgagctgtac tacttcgctg gtattgacga agcgcgcttc aagcgcccgg
    36961 tcgtgcctgg cgatcaaatg atcatggaag tcactttcga aaaaacgcgc cgcggcctga
    37021 cccgttttaa aggggttgct ctggtcgatg gtaaagtagt ttgcgaagca acgatgatgt
    37081 gtgctcgtag ccgggaggcc tgatacgtga ttgataaatc cgcctttgtg catccaaccg
    37141 ccattgtgga agagggcgcg tcaattggcg cgaacgcaca cattggtcct ttttgtatcg
    37201 ttggacccca tgtcgaaatt ggtgagggta ccgtactgaa atctcacgtt gtcgtgaatg
    37261 gtcatactaa aattggccgc gataatgaga tttatcagtt tgcctccatc ggcgaagtta
    37321 accaggattt gaaatatgct ggcgaaccga cccgtgtgga aatcggcgat cgtaaccgca
    37381 ttcgcgaaag cgtcaccatt catcgtggca cagtccaggg cggtggattg acgaaggtgg
    37441 gcagcgacaa cttactgatg atcaacgcgc acattgcgca cgattgtacg gtaggtaacc
    37501 gctgtattct cgccaacaac gcaacgctgg cgggtcacgt atcggttgac gacttcgcga
    37561 tcatcggcgg catgaccgca gtccatcagt tctgcatcat tggtgcgcat gtgatggttg
    37621 gcggctgctc cggtgtggcg caggacgtcc ctccttatgt tattgcgcag ggtaaccacg
    37681 caacgccgtt cggtgtcaat atcgaagggc tgaagcgccg cggattcagc cgtgaggcga
    37741 ttaccgctat ccgcaatgcg tataagctaa tttatcgtag cggtaaaacg ctcgatgaag
    37801 tgaaaccgga aattgctgaa ctggcggaaa catatccgga agtgaaagcc tttaccgatt
    37861 tctttgcacg ctcaacgcgc ggtctgattc gttaatgact gaccagcgtc cattaacgat
    37921 tgccctggtc gccggagaaa cctccggcga tatcctgggg gccggtttaa tccgcgctct
    37981 gaaagaacgt gtgcccaacg cccgctttgt tggtgttgcc gggccacgaa tgcaggctga
    38041 aggctgcgaa gcctggtacg aaatggaaga actggcggtg atgggcattg ttgaagtgct
    38101 cggtcgtctg cgtcgcttac tgcatattcg tgccgatctg acaaagcgtt ttggcgaact
    38161 gaagccagat gtttttgttg gtattgatgc gcccgacttc aatattactc ttgaaggtaa
    38221 cctcaaaaag cagggtatca aaaccattca ttacgtcagt ccatccgtct gggcgtggcg
    38281 acagaaacgc gttttcaaaa taggcagagc caccgatctg gtgctcgcat ttctgccttt
    38341 cgaaaaagcg ttttatgaca aatacaatgt accgtgccgc tttatcggtc ataccatggc
    38401 tgatgccatg ccgttagatc cagataaaaa tggtgcccgt gatgtgctgg ggatccctta
    38461 cgatgcccac tgtctggcat tgttgccggg cagccgtggt gcagaagtcg aaatgcttag
    38521 tgccgatttc ttgaaaactg cccagctttt gcgccagaca tatccggatc tcgaaatcgt
    38581 ggtgccgctg gtgaatgcca aacgccgcga gcagtttgaa cgcatcaaag ctgaagtcgc
    38641 gccagacctg tcggttcatt tgctggatgg aatgggccgt gaggcgatgg tcgccagcga
    38701 tgcggcacta ctggcgtcgg ggacggcagc cctggagtgt atgctggcga aatgcccgat
    38761 ggtggtggga tatcgcatga agccttttac cttctggttg gcgaagcggc tggtgaaaac
    38821 tgattatgtc tcgctgccaa atctgctggc gggcagagag ttagtcaaag agttattgca
    38881 ggaagagtgt gagccgcaaa aactggctgc ggcgctgtta ccgctgttgg cgaacgggaa
    38941 aaccagccac gcgatgcacg ataccttccg tgaactgcat cagcagatcc gctgcaatgc
    39001 cgatgagcag gcggcacaag ccgttctgga gttagcacaa tgatcgaatt tgtttatccg
    39061 cacacgcagc tggttgcggg tgtggatgaa gtcggacgcg ggccgttagt tggcgcggtc
    39121 gtcaccgctg cggtgatcct tgacccggcg cgcccgattg ccgggctgaa tgattccaaa
    39181 aagctgagcg aaaaacgccg tctggcgctc tatgaagaga tcaaagagaa agcgttgagc
    39241 tggagtctgg gccgcgcgga accccacgaa atcgacgagc tgaacattct tcatgcgacc
    39301 atgctggcga tgcagcgtgc cgtcgctggg ctgcatattg cgccggaata tgtgttgatt
    39361 gatggtaacc gctgcccgaa attaccgatg cctgcgatgg ctgtggtgaa aggggatagc
    39421 cgcgtaccag aaatcagtgc cgcgtctatc ctggcgaaag tgacgcgtga cgccgaaatg
    39481 gcggcgctgg atattgtttt cccgcaatat ggttttgccc aacataaagg gtatccaacc
    39541 gcttttcatc tggaaaaact ggctgaatat ggcgcgactg aacatcatcg tcgcagcttt
    39601 gggcctgtca aacgcgcact gggacttgcg tcctgattct tgtgtcgaga ttaagtaaac
    39661 cggaatctga agatgtctga accacgtttc gtacacctgc gggtgcacag cgactactcg
    39721 atgatcgatg gcttggccaa aaccgcaccg ctggtaaaaa aggcggcggc gttgggtatg
    39781 ccagcactgg cgatcaccga tttcaccaac ctttgcggtc tggtgaagtt ctacggagcg
    39841 ggacatggcg cagggattaa gcctatcgtc ggggcagatt ttaatgtcca gtgcgacctg
    39901 ctgggtgatg agttaaccca cctgacggta ctggcggcga acaataccgg ctatcagaat
    39961 ctggcgttgc tgatctcaaa agcgtatcag cgcgggtacg gtgccgccgg gccgatcatc
    40021 gatcgcgact ggcttatcga attaaacgaa gggttgatcc ttctttccgg cgggcgcatg
    40081 ggcgacgtcg gacgcagtct tttgcgtggt aacagcgcgc tggtagatga gtgtgtcgcg
    40141 ttttatgaag aacacttccc ggatcgctat tttctcgagc tgatccgcac cggcaggccg
    40201 gatgaagaaa gctatctgca cgcggcggtg gaactggcgg aagcgcgcgg tttgcccgtc
    40261 gtggcgacta acgatgtgcg ctttatcgac agcagcgact ttgacgcaca cgaaatccgc
    40321 gtcgcgatcc acgacggctt taccctcgac gatcctaaac gcccgcgtaa ctattcgccg
    40381 cagcaatata tgcgtagcga agaggagatg tgtgagctgt ttgccgacat cccagaagcc
    40441 cttgccaaca ccgttgagat cgccaaacgc tgtaacgtaa ctgtgcgtct tggtgaatac
    40501 ttcctgccgc agttcccgac cggggacatg agcaccgaag attatctggt caagcgtgca
    40561 aaagaaggcc tggaagagcg tctggcattt ttattccctg acgaggaaga acgtcttaaa
    40621 cgccgcccgg aatatgacga acgtctggag actgaacttc aggttatcaa ccagatgggc
    40681 ttcccgggct acttcctcat cgttatggaa tttatccagt ggtcgaaaga taacggcgta
    40741 ccggtagggc caggccgtgg ctccggtgcg ggttcactgg tggcctacgc gctgaaaatc
    40801 accgacctcg atccgctgga atttgacctg ctgttcgaac gtttccttaa cccggaacgt
    40861 gtctccatgc ctgacttcga cgttgacttc tgtatggaga aacgcgatca ggttatcgaa
    40921 cacgtggcgg acatgtacgg tcgtgatgcg gtatcgcaga ttatcacctt cggtacgatg
    40981 gcggcgaaag cggtgatccg cgacgtaggc cgcgtgctgg ggcatccgta cggctttgtc
    41041 gaccgtatct cgaaactgat cccgcccgat ccggggatga cgctggcgaa agcgtttgaa
    41101 gccgagccgc agctgccgga aatctacgaa gcggatgaag aagttaaggc gctgatcgac
    41161 atggcgcgca aactggaagg ggtcacccgt aacgccggta agcacgccgg tggggtggtt
    41221 atcgcgccga ccaaaattac cgattttgcg ccgctttatt gcgatgaaga gggcaaacat
    41281 ccggtcaccc agtttgataa aagcgacgtt gaatacgccg gactggtgaa gttcgacttc
    41341 cttggtttgc gtacgctcac catcatcaac tgggcgctgg agatgatcaa caagcggcgg
    41401 gcgaagaatg gcgagccacc gctggatatc gccgcgatcc cgctggatga caagaaaagc
    41461 ttcgacatgc tgcaacgctc ggaaaccacg gcggtattcc agcttgaatc gcgcggcatg
    41521 aaggacctga tcaagcgtct gcaacctgac tgcttcgaag atatgatcgc actggtggca
    41581 ctgttccgcc ctggtccgtt gcagtcaggg atggtggata actttatcga ccgtaaacat
    41641 ggtcgtgaag agatctccta tccggacgtg cagtggcagc atgaaagcct gaaatcggta
    41701 ctggagccaa cctacggcat tatcttgtat caggaacagg tcatgcagat tgcccaggtg
    41761 ctttctggtt ataccctcgg tggcgcggat atgctgcgcc gtgcgatggg taagaaaaag
    41821 ccggaagaga tggctaagca gcgttctgta tttgctgaag gtgcagaaaa gaacggaatc
    41881 aacgccgaac tggcgatgaa aatcttcgac ctggtggaga aattcgcggg ttacggattt
    41941 aacaaatcgc actctgcggc ctatgctttg gtgtcatatc aaacgttatg gctgaaagcg
    42001 cactatccgg cggagtttat ggcggcggta atgaccgccg atatggacaa caccgagaag
    42061 gtggtgggcc tggtggatga gtgctggcgg atggggctga aaatcctgcc accagatata
    42121 aactccggtc tttaccattt ccacgtcaac gacgatggcg aaatcgtgta tggtattggc
    42181 gccatcaaag gggtaggtga aggtccgatt gaggccatca tcgaagcccg taataaaggc
    42241 ggctacttcc gcgaactgtt tgatctctgc gcccgtaccg acaccaaaaa gttaaaccgg
    42301 cgggtgctgg aaaaactgat catgtccggg gcgtttgacc gtcttgggcc acaccgcgcg
    42361 gcgctgatga actcgctggg tgatgcgtta aaagcggcag atcaacacgc gaaagcggaa
    42421 gctatcggtc aggccgatat gttcggcgtg ctggccgaag agccggaaca aattgaacaa
    42481 tcctacgcca gctgccaacc gtggccggag caggtggtat tagatgggga acgtgaaacg
    42541 ttaggcctgt acctgaccgg acaccctatc aaccagtatt taaaagagat tgagcgttat
    42601 gtcggaggcg taaggctgaa agacatgcac ccgacagaac gtggtaaagt catcacggct
    42661 gcggggctcg ttgttgccgc gcgggttatg gtcaccaagc gcggcaatcg tatcggtatc
    42721 tgcacgctgg atgaccgttc cgggcggctg gaagtgatgt tgtttactga cgccctggat
    42781 aaataccagc aattgctgga aaaagaccgc atacttatcg tcagcggaca ggtcagcttt
    42841 gatgacttca gcggtgggct taaaatgacc gctcgcgaag tgatggatat tgacgaagcc
    42901 cgggaaaaat atgctcgcgg gcttgctatc tcgctgacgg acaggcaaat tgatgaccag
    42961 cttttaaacc gactccgtca gtctctggaa ccccaccgct ctgggacaat tccagtacat
    43021 ctctactatc agagggcgga tgcacgcgcg cggttgcgtt ttggcgcgac gtggcgtgtc
    43081 tctccgagcg atcgtttatt aaacgatctc cgtggcctca ttggttcgga gcaggtggaa
    43141 ctggagtttg actaatacag gaatactatg agtctgaatt tccttgattt tgaacagccg
    43201 attgcagagc tggaagcgaa aatcgattct ctgactgcgg ttagccgtca ggatgagaaa
    43261 ctggatatta acatcgatga agaagtgcat cgtctgcgtg aaaaaagcgt agaactgacg
    43321 cgtaaaatct tcgccgatct cggtgcatgg cagattgcgc aactggcacg ccatccgcag
    43381 cgtccttata ccctggatta cgttcgcctg gcatttgatg aatttgacga actggctggc
    43441 gaccgtgcgt atgcagacga taaagctatc gtcggtggta tcgcccgtct cgatggtcgt
    43501 ccggtgatga tcattggtca tcaaaaaggt cgtgaaacca aagaaaaaat tcgccgtaac
    43561 tttggtatgc cagcgccaga aggttaccgc aaagcactgc gtctgatgca aatggctgaa
    43621 cgctttaaga tgcctatcat cacctttatc gacaccccgg gggcttatcc tggcgtgggc
    43681 gcagaagagc gtggtcagtc tgaagccatt gcacgcaacc tgcgtgaaat gtctcgcctt
    43741 ggcgtaccgg tagtttgtac ggttatcggt gaaggtggtt ctggcggtgc gctggcgatt
    43801 ggcgtgggcg ataaagtgaa tatgctgcaa tacagcacct attccgttat ctcgccggaa
    43861 ggttgtgcgt ccattctgtg gaagagcgcc gacaaagcgc cgctggcggc tgaagcgatg
    43921 ggtatcatcg ctccgcgtct gaaagagctg aaactgatcg actccatcat cccggaaccg
    43981 ctgggcggcg ctcaccgtaa cccggaagcg atggcggcat cgttgaaagc gcaactgctg
    44041 gcggatctgg ccgatctcga tgtgttaagc actgaagatt taaaaaatcg tcgttatcag
    44101 cgcctgatga gctacggtta cgcgtaattc gcaaaagttc tgaaaaaggg tcacttcggt
    44161 ggcccttttt tatcgccacg gtttgagcag gctatgatta aggaaggatt ttccaggagg
    44221 aacacatgaa catcattgcc attatgggac cgcatggcgt cttttataaa gatgagccca
    44281 tcaaagaact ggagtcggcg ctggtggcgc aaggctttca gattatctgg ccacaaaaca
    44341 gcgttgattt gctgaaattt atcgaacata accctcgaat ttgcggcgtg atttttgact
    44401 gggatgagta cagtctcgat ttatgcagcg atatcaatca acttaatgaa tatctcccgc
    44461 tttatgcctt catcaacacc cactcgacga tggatgtcag cgtgcaggat atgcggatgg
    44521 cgctctggtt ttttgaatat gcgctggggc aggcggaaga tatcgccatt cgtatgcgtc
    44581 agtacaccaa cgaatatctt gataacatta cgccgccgtt cacgaaagcc ttgtttacct
    44641 acgtcaaaga gcggaagtac accttttgta cgccggggca tatgggcggc accgcatatc
    44701 aaaaaagccc ggttggctgt ctgttttatg attttttcgg cgggaatacc cttaaggctg
    44761 atgtctctat ttcggtcacc gagcttggtt cgttgcttga ccacaccggg ccacacctgg
    44821 aagcggaaga gtacatcgcg cggacttttg gcgcggaaca gagttatatc gttaccaacg
    44881 gaatatcgac gtcgaacaaa attgtgggta tgtacgccgc gccatccggc agtacgctgt
    44941 tgatcgaccg caactgccat aaatcactgg cgcatctgtt gatgatgaac gatgtagtgc
    45001 cagtctggct gaaaccgacg cgtaatgcgt tggggattct gggggggatc ccgcgccgtg
    45061 aatttactcg cgacagcatc gaagagaaag tcgctgccac tacgcaagca caatggccgg
    45121 ttcatgcggt gatcaccaac tccacctatg atggtttgct ctacaacacc gactggatca
    45181 aacagacgct ggatgtcccg tcgatccact tcgattccgc ctgggtgccg tatactcatt
    45241 ttcatccaat ctaccagggt aaaagtggta tgagcggcga gcgtgttgcg ggaaaagttt
    45301 tcttcgaaac gcaatcgacc cacaaaatgc tggcggcgtt atcgcaggct tcactgatcc
    45361 acattaaagg cgagtatgac gaagaggcgt ttaacgaagc ctttatgatg cataccacca
    45421 cctcgcccag ttatcccatt gttgcttcgg ttaagacggc ggcggcgatg ctgcgtggta
    45481 atccgggcaa acggctgatt aaccgttcgg tggagcgggc gctgcatttt cgcaaagagg
    45541 tccagcggct gcgggaagag tctgacggtt ggtttttcga tatctggcaa ccgccgcagg
    45601 tggatgaagc cgaatgcagg cccgttgcgc ctggcgaaca atggcacggc tttaacgatg
    45661 cggatgccga tcatatgttt ctcgatccgg ttaaagtcac tattttgaca ccggggatgg
    45721 acgaacaggg caatatgagt gaggagggga tcccggcggc gctggtagca aaattcctcg
    45781 acgaacgtgg gatcgtagta gagaaaaccg gcccttataa cctgctgttt ctctttagta
    45841 ttggcatcga taaaaccaaa gcaatgggat tattgcgtgg gttgacggaa tttaagcgct
    45901 cttacgatct caacctgcgg atcaaaaata tgctgcccga tctctatgct gaagatcccg
    45961 atttctaccg caatatgcgt attcaggatc tggcgcaagg gatccataag ctgattcgta
    46021 aacacgatct ttccggtttg atgttgcggg cattcgatac tttgccggaa atgatcatga
    46081 cgccacatca ggcatggcag agacagatta aaggtgaagt agaaaccatt gcgctggaac
    46141 aactggtcgg tagagtatcg gcaaatatga tcctgcctta tccaccgggc gtaccgctgt
    46201 tgatgccagg agaaatgctg accaaagaga gccgcacggt actcgatttt ctactgatgc
    46261 tttgttccgt agggcaacat taccccggtt ttgaaacgga tattcacggc gcgaaacagg
    46321 acgaagacgg cgtttaccgc gtacgagtcc taaaaatggc gggataactt gccagagcgg
    46381 cttccgggcg agtaacgtgc tgttaacaaa taaaggagac gttatgctgg gtttaaaaca
    46441 ggttcaccat attgcgatta ttgcgacgga ttatgcggtg agcaaagctt tctactgcga
    46501 tattcttggt ttcacgctgc aaagcgaagt ctatcgcgaa gcgcgcgact cgtggaaagg
    46561 ggatttggcg cttaatgggc aatatgtgat tgagcttttc tcttttccgt tcccgccgga
    46621 gcgccccagc cgaccggaag cttgcggcct gcgtcatctg gcttttagcg ttgatgacat
    46681 cgatgcggca gtggcgcacc ttgaaagcca taacgtgaag tgtgaagcca tccgtgtcga
    46741 tccatacacg caaaaacgct tcaccttctt taacgatccg gacgggctgc cgttggaact
    46801 gtatgagcag taaggcttgt catcgccgca tttgcccggt aacgtgccgg gcattgctac
    46861 tgtaaaatcg caccatcatg acactcacgc tcaatagaca acttctcacc tcacgccaga
    46921 ttctggtggc ctttagcggc gggcttgact ccaccgttct gctgcatcag ttggtgcagt
    46981 ggcggacgga aaatccgggc gtcactctgc gcgctattca tgtgcatcac ggtttaagtg
    47041 ccaatgccga tgcctgggtt acgcattgcg aaaacgtctg ccaacagtgg caggtgccgc
    47101 tggtggtcga acgcgtacaa cttgcgcaag aggggctggg gattgaggcc caggcgcggc
    47161 aggcacgtta tcaggcattt gcccgtaccc tattgcccgg cgaagtgctg gtcaccgcgc
    47221 aacatctcga cgatcaatgt gaaacctttc tgctggcgct aaaacgcggt agcggccctg
    47281 ccgggctttc ggctatggcg gaagtttcgg agtttgctgg aacacggctt atccgcccgt
    47341 tgcttgcccg cacgcggggg gaactggagc agtgggcatt ggcgcatggt ttacgctgga
    47401 ttgaagacga aagtaatcag gacgactcat acgatcgtaa ctttctgcgc ctgcgcgtag
    47461 tgccgttatt gcagcagcgt tggccgcatt ttgcggaagc aacggcccgc agcgccacac
    47521 tttgtgctga acaagagagt ctgttggatg aactgctggc ggatgattta gcacactgcc
    47581 aaacgtcgca ggggacgctg cagattgcgc caatgctggc gatgagtgat gcccgtcgcg
    47641 cggcgattat tcgccgctgg ctggcagggc agaatgcacc gatgccttcc cgcgacgcgc
    47701 tggtgcgcat ctggcaggaa gtggcgctgg cgcgggaaga tgcttccccc tgtttacgtt
    47761 tgggcgcctt tgaaatccgc cgctatcagt cgcaactgtg gtggattaaa tccgtcaccg
    47821 ggcaaagcga aaccattgtg ctgtggcaga cgtggctaca accgctggaa ttaccggcgg
    47881 ggctgggaac cgtacagctt actgcgggag gcgatattcg ccctccgcgt gcagacgaag
    47941 cggtcagcgt gcgtttcaaa gcgccaggat tgctgcatat tgtcgggcgc aacggcggac
    48001 gtaagctgaa gaaaatctgg caagagttag gcgtgccgcc gtggctacgt gacaccacgc
    48061 cactgctgtt ttatggagaa acgctgattg cggcggcagg ggtatttgtg acgcaagaag
    48121 gtgtggctga aggtgagaat ggcgtaagtt ttgtctggca gaaaacgctt agttaagtga
    48181 aagccggata agacgcatca aacgtcgcat ccggcgaaag tcaatcagga ttcgcttacc
    48241 accaccgtac cgatttcagg gtggctaaag ctggtaattt tatccagacg cagctcgcgg
    48301 gtttcgccag cggcctcgac gaccagatac tccacatttt tgcgggagac taaatcactg
    48361 gcttttgcct gcaatttttc gccatctttc agctcaagtg tcagcattaa atgatgctgg
    48421 caggcgagct caagattatc gtaatcatca caattaattg gttgatacgt atcattcatt
    48481 gacataatcg ctcaccagta agtttgccgc agcgtatgct gctttttccc tgacagcctc
    48541 agaaagggcg tcgtcggcag ccatttcatt cagcactttc aaaacgcagc ccagcgcgtc
    48601 cggaacgtat cctaagtctc cgctggcgat ttccgcgtac cgcttgcgta ttaactcaca
    48661 atatttttcc acatgccctc ctgtcagcac tctgacttaa ccgtggatgc aagtctaagc
    48721 ctacgaagat aaactctgtt tcgcaaggtg actataccac actcatttct gcaatatcag
    48781 cgccgcaact gcacgtattc cgttacaatg gcctcctgat tcgaaaggag ttttcttatg
    48841 gcgcttaaag cgacaattta taaagcgacg gttaatgtgg ccgatctcga ccgcaaccag
    48901 tttctcgatg cctctctgac gctggcgcgc cacccttcag aaacccagga gcgtatgatg
    48961 ctgcgcttgc tggcgtggct gaaatatgct gatgaacgtc tgcaatttac ccgtggtttg
    49021 tgtgccgatg atgagccgga agcgtggctg cgtaacgatc acctgggcat tgatttgtgg
    49081 attgagctgg ggctgccgga tgagcgacga attaagaaag cctgcaccca ggccgcagaa
    49141 gtggcgctgt ttacctataa tagtcgggcg gcgcaaatct ggtggcagca aaatcagagc
    49201 aaatgtgtgc agtttgccaa tctttccgtc tggtatctgg acgatgaaca actggcgaaa
    49261 gtaagcgcct ttgccgatcg taccatgacg ctgcaggcaa cgattcagga tggtgttatc
    49321 tggttatcgg atgataagaa taatctggaa gttaacttaa ccgcctggca acaaccttca
    49381 tgattgtgat ttcccgacat gttgctattc ccgatggtga gcttgagatc accgccattc
    49441 gtgcgcaggg cgctggcggg cagcatgtta ataagacctc aacggctatt catctgcgtt
    49501 ttgacattcg ggcgtccagc ctgccagagt attacaaaga gcgtctgctc gccgccagcc
    49561 atcatttgat cagcagtgat ggcgtgattg tcattaaggc acaggaatac cgcagtcagg
    49621 aactgaaccg cgaagccgct ctggcccggc tggtggcagt gattaaagat ttaacaaaag
    49681 aacaaaaagc ccgacgaccc acgcggccca cccgtgcatc gaaagagcgc aggctggcat
    49741 cgaaagcaca aaaatcaagc gtgaaggcga tgcgcggcaa agtacgcagc ggtcgggaat
    49801 aaaaagaagg aatggatggt gaaaaaagcg atagtgacag cgatggctgt aatcagcctc
    49861 tttactctga tgggatgtaa taatcgggcc gaagtcgata cgctttctcc ggcgcaggct
    49921 gccgaactga aaccgatgcc gcaaagttgg cgcggcgtgc taccgtgtgc cgattgcgaa
    49981 ggaatcgaaa tctctctgtt cctcgaaaaa gacggaacat gggtgatgaa tgagcgttat
    50041 ctcggtgctc gtgaagaacc ttcctccttc gcttcctacg gtacatgggc gcgaaccgct
    50101 gacaagctgg tattaaccga cagcaaaggt gaaaagtcat attatcgggc gaaaggcgat
    50161 gcgctggaga tgctcgatcg tgaaggcaat ccaattgaat cgcagttcaa ctatacgctg
    50221 gaaccggcac aatccagttt acctatgacg ccgatgaccc tgcgaggcat gtatttttat
    50281 atggctgatg cggcgacctt cactgattgc gcgaccggaa aacgtttcat ggtagcgaat
    50341 aacgcagagc tggagcgtag ctacctggct gcgcgcggtc acagtgaaaa accggtgtta
    50401 ctgtcagtag aaggtcactt tacgcttgag gctaatccgg atactggtgc gccaaccaaa
    50461 gtattagcgc ccgatacggc gggtaaattt tacccgaata aggattgcag tagtttgggg
    50521 cagtaaacct gcttaaggca gaaacaaacg caaaattgcc tgatgcgctg cgcttatcag
    50581 gcctacagca tataatgcaa cattttgaat tagcacgatt ttgtaggccg gataaggcgt
    50641 ttacgccgca tccggcattt tgtgcacgag gctggcttta tcactgagtc agcccaacaa
    50701 agcgacccgc cttgatataa atcccaggct tcaggtgccc aacatactgt aactgcgttt
    50761 ctggcttaaa cgcagagaca tcaccaatac gcatatgcat cagatcggca gggctaatcc
    50821 agcccgattg cgccagtgta agtggatgcc cggatttggc aaatgcatca gtgacaaatt
    50881 ccgaacaaaa ccacgacttt ttgtctcctt cgcacacact gcttaactgc gctttcgcca
    50941 ggtcgctgac gcactgttgg cgaaaatcct cggagaacgg attcagtgag cacatctggc
    51001 gagtcaccat aaagggaata aattcgacaa tgccgcgata gttataaccg ctatctttga
    51061 ttttattggc aaacgcggtg atatctgtgg cttgttgcgg ggtaagatcc gggactcgta
    51121 agacgaaaag cttatcacta tgcttcatcg cttttttaag ggaaacaatc tggacgccag
    51181 cgcctgtcgc ttctgcaacg ttattatcac ccagaaagat tgcaacgtga ctcacagagg
    51241 aagtgctgaa gacgcggatt ccaaatgagg ttaccccaag gcttgaggag aacagcaaat
    51301 cgccgggttt gagatcaggt gccgttattt cttttattga ttgttcggtg aaagagcttt
    51361 gatgctggaa tttgacagcc catgtttttg cctcggcatc tactgctgtg gctgatgggt
    51421 ctggctggct gatatcaacg gtacaagccg aaagtaaaag aaagctgggg agaagcaggc
    51481 ggcagtacgc ctttggttta tccattttat acaatccatg taaaaaaggg ccctgaaatt
    51541 caggaccctt tctggcatca gcctttaatc tgtttcacca gatattcgac gatgtcacca
    51601 gtcttaatta actgtttctc gccgttgcga cgatatttat attcgatatc gtcgttgtcg
    51661 aggttacggt cgcccagcac aatagtgtgc ggaataccga tcagttccat atcagcaaac
    51721 atcacgcccg gacgctcttt gcggtcatcc agcagcactt cgataccttg tgcacgcagt
    51781 tcgctgtaca gtttctcagc aagctcttgt acgcggaagg atttgtgcat gttcatcggc
    51841 agaatcgcca cctggaacgg cgcgatagcg tcaggccata cgatgccgcg ttcgtcgtag
    51901 ttctgctcaa tcgccgcagc taccacacgc gttaccccga taccgtagca acccatcgtc
    51961 aggatttggt tacggccatc ttcaccctgt acggaggctt tcagtgcttc ggagtacttg
    52021 gtacccagct ggaagatgtg accaacttcg ataccacgtt tgatcagcag cgtaccctgg
    52081 ccatccgggc ttggatcgcc agccaccacg ttacggatat ctgcaacttc cggggtagcg
    52141 acatcgcgat cccagttgat accgaagtag tgtttaccat cgatgttagc accagcagcg
    52201 aaatcactca tcgccgcaac ggtacggtca atcaccaccg gaatcggcat gtttaccgga
    52261 cccagtgaac ccggaccggc tttaaccaca gcacgaattt cttcttcggt cgcgaaagtc
    52321 agcgggcttg caacctgcgg cagtttttct gctttaactt cgttcagctc gtgatcaccg
    52381 cgcaccagca gcgcaaccag cgggaagcta ctgccttcaa ccgctttaac cagcagagtc
    52441 ttaaccgttt tctcaatcgg cagattgaac tgttcaacca gttccgcgat ggttttcgcg
    52501 ttcggcgtat caaccagcgt catttcctgg gtagcagcag cgcgcggttc tttcggcgcg
    52561 atagcttctg ccagttcaat gttcgctgca tagtcagagg tgtcggagaa gaccacatcg
    52621 tcttcaccgc tctgcgccag cacctggaat tcgtgagagg cgctgccgcc gatagaaccg
    52681 gtgtcggcct gtacggcgcg gaaatccagc cccatgcggc tgaagatttt gctgtaggcc
    52741 gcatacattg catcgtaggt ttcctgcagg gattcctgag aagtatggaa agagtaagca
    52801 tctttcatca ggaattcgcg ggaacgcatg acgccgaaac gcggacgcac ttcgtcgcgg
    52861 aacttggtct ggatctgata gaagttcagc ggcagctgtt tgtaagagct aagctcgtta
    52921 cgaatcaggt cagtgataac ttcttcatga gttgggccga gtacgaacgg acgctcgcca
    52981 cggtcaacaa aacgcagcag ttccggaccg tactgttccc aacgaccact ctcttgccac
    53041 aaatcggctg gctgaaccac cggcatcgac acctcgatcg caccggcgtt gttcatctct
    53101 tcacgcacga tgttttcgac ttttttcaga acgcgcacgc cggtcggcag ccaggtatat
    53161 aacccggagg ccagcttgcg gatcatcccg gcgcgcagca tcagctgatg gctgatcacc
    53221 tcggcgtcgg caggtgtctc cttgagagtg gagagcaggt attggctagt acgcatgttg
    53281 ttacggttcc agttggaagg tagaacaggc tcaaggcgag cctgggacaa aaaaagtgat
    53341 ttagtttacc agtgcaaaag aaatgtcaaa agagaagggc gtgaatttaa cgcggttcca
    53401 gcgcaaagac ttcaaaacct gcgtcggtga cgcgccagcg aacgttaaaa tcatgtagcc
    53461 agacggcata ggttttgccc gtttcctcac ctttacgata ggccgggcgc gggtcctgcg
    53521 ccagtacttc gcggataaac agcgttaact gcggataacg cttctccagc gtcaaaagct
    53581 gcttttcgac ctctgcggta aaactcaccg ccatctctgc agctggcgca ctttgcgcat
    53641 agctggcact ggcatcggga agcgattcgg caaaggggag atacggtttg atatccacta
    53701 ccggcgtacc atcgaccaga tccagactgc cgagcttcag aatcacgctg cctttatggc
    53761 aaacaacctc tttcagctct accagcgaca tgccaattgg gttagggcgg aaagtagagc
    53821 gtgttgcgaa aacccccatt ctggcgttac cgccgaggcg cggtggacgc acagtcggac
    53881 gccagccgcc ttccatcgtt tgatgaaaga cgaaaaggat ccataaatgg ctgaatgctt
    53941 ccaggccgcg aacggcgtcg gcctggttgt aggaagctat gagatgcagt tcgccgttgg
    54001 cgctttttac cagacctggc tggcgcggaa cggcgaattt ttctttatag ggcgagcgaa
    54061 taacgcctat ttgctcgaac tggaaactgc tcatttcgcc gtaatgttaa gcgcagagcc
    54121 gatacataca gcctgacgat agcagcccgg cgtaccgctg gtgacttcgc agctatgcag
    54181 taatacagca ttggctttca ttttagaggc attgatttgc atctgcttac gtgcggttgg
    54241 aatgctcggc ggagagtcct gattagaggc ctggcaagag tcgccactga cttcaccgag
    54301 atcgcggaac ggtttgccga ctaattcttc tgcattggta taaattcgga ccggcgtggc
    54361 gcgcggcgct ttcggttttg caggctccgc tttcggctgg ggtgcagtgc tttgaacggg
    54421 ttcgacaggg gatctgctta acatggaaca gccgcttagc atgagtgcta ctaaacagat
    54481 cggtaaagca cgcatagtat ttcctcaatg tatgatcaaa acgtcaatat tgaatcagga
    54541 gcttgtaaaa atgacaagac gggcaagcgc ccgtcctgaa tgatattaca aattgtggaa
    54601 acagcctaaa aattaccagc ctttaacagc gccgccgtta aacactttgt ttgctgcttc
    54661 gtaaacttca tcagactgat aagcctggac gaatttcttc acgttctcgg cgtctttgtt
    54721 atcttcacgc gttacgatca ggtttacgta tggggagtct ttatcttcga caaagatacc
    54781 gtctttcgcc ggagtcaggc caatctggct ggcataggtg gtattgataa ctgccagagc
    54841 gatttgcgcg tcgtccagag aacgcggcag ttgcggtgct tccagttcaa caattttcag
    54901 attttttggg ttctcaacaa catcaagaac ggtcggcagc aggccaacgc catctttcag
    54961 tttgatcaag cccacttttt gcagcagcag cagtgaacga ccaagattag ttggatcgtt
    55021 tggcacggca acctgcgaac catcctgcag ttcatccagt gatttgattt tcttggagta
    55081 accagcaatc ggataaacaa aagtgttgcc tactgcgacc agtttgtagc cacgatcttt
    55141 cagttgctga tcaaggtacg gtttatgctg gaaggcgttg gcgtcgatat cgcctttgct
    55201 caatgcttcg tttggcagaa catagtcgtt gaaggttacc agctcaacgt ccaggccata
    55261 tttgtctttc gcaactttct gcgcgacttc tgctacctgc tgttcggcac caacaatcac
    55321 gccgacttta atgtggtttg gatctttttc atcctgaccg cagcctacca gtgccagtga
    55381 tccaatcagg gctcccactg ccgcaaaggt tttgaatttg aacgccatac cttattcctt
    55441 cttcaattat ttatgttgtg ttgaacgtta cttgcgagtg acagcccgga cgatgcggtc
    55501 gcctgcaaac tgaattaaat aaaccagaat gaccagcaat accagtaccg tattcatcac
    55561 cgtcgcgttg tagccgatgt agccatactg atagccaatc tgacctaaac caccggcacc
    55621 gactgcacca cccatcgcgg aataaccgac cagggtaatc agggtgatag ttgccgcatt
    55681 caccagaccc ggcaacgctt ccggtaacag cactttacag acgatctgca ttggcgtggc
    55741 acccattgcg cgggaagctt caattaaccc ggttgggatc tccagcagag cgttctcgac
    55801 catacgggca ataaacggtg ctgcaccaac ggttaacgga acaattgctg cctgcaaacc
    55861 aatcgatgta ccgacaataa cgcgggtaaa cggaatcatc cataccagca agataatgaa
    55921 cgggatggaa cggaaaatgt tcacaatcgc agaaacggta cgatacagct tcgcgttagc
    55981 aataatttgc cccggacgcg tgacataaag cagaacgcca accggcagac caatcacaaa
    56041 cccaaaaaat ccggatacga aggtcattgc cagcgtttcc catacgccac gaaccagcag
    56101 ccacatcatc ggctcagaca taacccagta cctctacttt tacatggtgt tcctgcagcc
    56161 aggcaatggc ggcttgcgta tcttgttgtg tgccgtgcat ttcagtcagc atgatgccga
    56221 atttaacgcc accggcgtag tccatctgcg cgctaataat gttgttgttg acgttgaaac
    56281 gacgcgcggt ttcagaaagc agtggggcat cgaccgattg accggtaaac tccagacgca
    56341 gcatcggcac gcagtcagta aatggctccg tttgcagacg ttcctggtaa tcttccggga
    56401 tatccagatg cagggtcgac tgaataaact tctgcgccag cggcgttttc ggatgcgaga
    56461 acacttcact taccgtgtcc tgctcgatca gttcgccatt gctgatgacc gccacgcaat
    56521 cacaaatacg cttcacaacg tccatttcgt gggtgatcaa cagaatcgtc aaacccagac
    56581 ggcggttaat gtctttcagc agttcgagaa tagaacgtgt cgttgccggg tccagcgcgc
    56641 tggtggcttc atcacacagc aatactttgg gattgctggc taatgcacgg gcaatcgcca
    56701 cacgttgttt ctgcccaccg gaaagattcg acgggtagct atcatgctta tcgccaagac
    56761 caaccaatga cagcaattcc gtcacgcgac gtttgatctc gtctttcggt gtgttgtcca
    56821 gctccagcgg cagagccacg ttgccaaaaa cagtacgcga agagagcagg ttaaaatgct
    56881 ggaaaatcat accaatctgg cggcgagctt tggtcaactc ggattctgac agcgtggtca
    56941 attcctggcc atcgaccagc acgctaccct cggttgggcg ctccagcaag tttacacaac
    57001 gtataagcgt actcttaccc gcgcctgagg caccgataac gccataaatt tgtccagctg
    57061 gcacatgcag gctgacgttg ttcaacgcct ggatggtgcg ggtgccctgg tggaacactt
    57121 tggtgatatt cgaaagtttt atcattgatt atttattatc gtcattaagt tagtcgtagc
    57181 atctcgaatg cctgaaacgg gcaacgccgt caatgaaatg gatgttaagg catccagacg
    57241 tctaaatcaa tcaggtttat gcgaagagca ctttcttgca ggtcgaaaca tgcgatacta
    57301 gcgtcccatg ccttattaag gagctataaa aggtggcgaa gagcgtaccc gcaatttttc
    57361 ttgaccgtga tggcaccatt aatgtcgatc acggctatgt ccatgagatc gacaactttg
    57421 aatttatcga cggtgttatt gacgccatgc gcgagctaaa aaaaatgggc tttgcgctgg
    57481 tggtagtaac caaccagtct ggcattgctc gcggtaaatt taccgaagca cagtttgaaa
    57541 cgctgaccga gtggatggac tggtcgctgg cggaccgaga tgtcgatctg gatggtatct
    57601 attattgccc gcatcatccg caggggagtg ttgaagagtt tcgccaggtc tgcgattgcc
    57661 gcaaaccaca tccggggatg cttttgtcag cacgcgatta tttgcatatt gatatggccg
    57721 cttcttatat ggtgggcgat aaattagaag atatgcaggc agcggttgcg gcgaacgtgg
    57781 gaacaaaagt gctggtgcgt acgggtaaac ctattacacc tgaagcagaa aacgcggcag
    57841 attgggtgtt aaatagcctg gcagacctgc cgcaagcgat aaaaaagcag caaaaaccgg
    57901 cgcaatgatt aaaagatgaa cggttgaaat aaaaatgcat ttttccgctt gtcttcctga
    57961 gccgactccc tataatgcgc ctccatcgac acggcggatg tgaatcactt cacacaaaca
    58021 gccggttcgg ttgaagagaa aaaatcctga aattcagggt tgactctgaa ataggaaagt
    58081 gtgatatacg ccacctcgcg acagtgagct gaaagctgcg tcgcaactgc tctttaacaa
    58141 tttatcagac aatctgtgtg ggcactcgaa gatacggatt cttaacgtcg caagacga
//
LOCUS       DAZPAB010000005        55418 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZPAB010000005 DAZPAB010000000
VERSION     DAZPAB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.005"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="ACHUPG_000264"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="ACHUPG_000264"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR0860036.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="ACHUPG_000265"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="ACHUPG_000265"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR0860037.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="ACHUPG_000266"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="ACHUPG_000266"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR0860038.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="ACHUPG_000267"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="ACHUPG_000267"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR0860039.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="ACHUPG_000268"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="ACHUPG_000268"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR0860040.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="ACHUPG_000269"
     CDS             7826..9559
                     /locus_tag="ACHUPG_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR0860041.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="ACHUPG_000270"
     CDS             9708..10559
                     /locus_tag="ACHUPG_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0860042.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="ACHUPG_000271"
     CDS             complement(10546..10887)
                     /locus_tag="ACHUPG_000271"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR0860043.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="ACHUPG_000272"
     CDS             complement(10889..11767)
                     /locus_tag="ACHUPG_000272"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR0860044.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="ACHUPG_000273"
     CDS             complement(11733..14030)
                     /locus_tag="ACHUPG_000273"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR0860045.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="ACHUPG_000274"
     CDS             complement(14081..14401)
                     /locus_tag="ACHUPG_000274"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR0860046.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="ACHUPG_000275"
     CDS             complement(14416..15495)
                     /locus_tag="ACHUPG_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR0860047.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..18305
                     /gene="ptsP"
                     /locus_tag="ACHUPG_000276"
                     /pseudo
     CDS             15804..18305
                     /gene="ptsP"
                     /locus_tag="ACHUPG_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="ACHUPG_000277"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="ACHUPG_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="ACHUPG_000278"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="ACHUPG_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20973
                     /locus_tag="ACHUPG_000279"
                     /pseudo
     CDS             20366..20973
                     /locus_tag="ACHUPG_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="ACHUPG_000280"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="ACHUPG_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR0860048.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="ACHUPG_000281"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="ACHUPG_000281"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR0860049.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="ACHUPG_000282"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="ACHUPG_000282"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR0860050.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            25474..25608
                     /locus_tag="ACHUPG_000283"
     CDS             25474..25608
                     /locus_tag="ACHUPG_000283"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0860051.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="ACHUPG_000284"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="ACHUPG_000284"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR0860052.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="ACHUPG_000285"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="ACHUPG_000285"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR0860053.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="ACHUPG_000286"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="ACHUPG_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR0860054.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="ACHUPG_000287"
     CDS             30120..30728
                     /locus_tag="ACHUPG_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR0860055.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="ACHUPG_000288"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="ACHUPG_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR0860056.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="ACHUPG_000289"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="ACHUPG_000289"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR0860057.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="ACHUPG_000290"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="ACHUPG_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR0860058.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="ACHUPG_000291"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="ACHUPG_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR0860059.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="ACHUPG_000292"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="ACHUPG_000292"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR0860060.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="ACHUPG_000293"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="ACHUPG_000293"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR0860061.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="ACHUPG_000294"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="ACHUPG_000294"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR0860062.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="ACHUPG_000295"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="ACHUPG_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR0860063.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="ACHUPG_000296"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="ACHUPG_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR0860064.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="ACHUPG_000297"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="ACHUPG_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="ACHUPG_000298"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="ACHUPG_000298"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR0860065.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="ACHUPG_000299"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="ACHUPG_000299"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR0860066.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="ACHUPG_000300"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="ACHUPG_000300"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR0860067.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="ACHUPG_000301"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="ACHUPG_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR0860068.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="ACHUPG_000302"
     CDS             complement(44843..45142)
                     /locus_tag="ACHUPG_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR0860069.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="ACHUPG_000303"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="ACHUPG_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="ACHUPG_000304"
     CDS             45895..46494
                     /locus_tag="ACHUPG_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR0860070.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="ACHUPG_000305"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="ACHUPG_000305"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR0860071.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="ACHUPG_000306"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="ACHUPG_000306"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR0860072.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="ACHUPG_000307"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="ACHUPG_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR0860073.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="ACHUPG_000308"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="ACHUPG_000308"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR0860074.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="ACHUPG_000309"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="ACHUPG_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR0860075.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="ACHUPG_000310"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="ACHUPG_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR0860076.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="ACHUPG_000311"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="ACHUPG_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR0860077.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="ACHUPG_000312"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="ACHUPG_000312"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR0860078.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="ACHUPG_000313"
     CDS             complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="ACHUPG_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR0860079.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13493 a        15017 c        14164 g        12744 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttat attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt tagtttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatcgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatatggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg gatcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgatgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcac gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaacacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtatg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggtgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttat tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    55081 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    55141 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    55201 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    55261 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    55321 ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    55381 tagcaaaccg gacgaacgtt agttcatccg gctgtggg
//
LOCUS       DAZPAB010000006        49829 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZPAB010000006 DAZPAB010000000
VERSION     DAZPAB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.006"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..1224
                     /locus_tag="ACHUPG_000314"
     CDS             <1..1224
                     /locus_tag="ACHUPG_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR0860080.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="glpX"
                     /locus_tag="ACHUPG_000315"
     CDS             1221..2186
                     /gene="glpX"
                     /locus_tag="ACHUPG_000315"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR0860081.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="ACHUPG_000316"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="ACHUPG_000316"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFR0860082.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="ACHUPG_000317"
     CDS             2714..3427
                     /locus_tag="ACHUPG_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR0860083.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="ACHUPG_000318"
     CDS             complement(3399..4076)
                     /locus_tag="ACHUPG_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0860084.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="ACHUPG_000319"
     CDS             complement(4070..4747)
                     /locus_tag="ACHUPG_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0860085.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5442)
                     /locus_tag="ACHUPG_000320"
                     /pseudo
     CDS             complement(4735..5442)
                     /locus_tag="ACHUPG_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
     gene            complement(5443..6021)
                     /locus_tag="ACHUPG_000321"
     CDS             complement(5443..6021)
                     /locus_tag="ACHUPG_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR0860086.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="ACHUPG_000322"
                     /pseudo
     CDS             complement(6044..6475)
                     /locus_tag="ACHUPG_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="ACHUPG_000323"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="ACHUPG_000323"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR0860087.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="ACHUPG_000324"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="ACHUPG_000324"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR0860088.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="ACHUPG_000325"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="ACHUPG_000325"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR0860089.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /gene="mscS"
                     /locus_tag="ACHUPG_000326"
     CDS             10564..11424
                     /gene="mscS"
                     /locus_tag="ACHUPG_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR0860090.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="ACHUPG_000327"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="ACHUPG_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR0860091.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="ACHUPG_000328"
     CDS             12291..13031
                     /locus_tag="ACHUPG_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR0860092.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14148
                     /locus_tag="ACHUPG_000329"
     CDS             13198..14148
                     /locus_tag="ACHUPG_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0860093.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACHUPG_000330"
                     /pseudo
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACHUPG_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACHUPG_000331"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACHUPG_000331"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR0860094.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACHUPG_000332"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACHUPG_000332"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR0860095.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACHUPG_000333"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACHUPG_000333"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR0860096.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACHUPG_000334"
     CDS             complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACHUPG_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR0860097.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="ACHUPG_000335"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="ACHUPG_000335"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR0860098.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /gene="serA"
                     /locus_tag="ACHUPG_000336"
     CDS             21915..23147
                     /gene="serA"
                     /locus_tag="ACHUPG_000336"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR0860099.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="ACHUPG_000337"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="ACHUPG_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR0860100.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="ACHUPG_000338"
     CDS             23768..23827
                     /locus_tag="ACHUPG_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0860101.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23928..24530)
                     /locus_tag="ACHUPG_000339"
     CDS             complement(23928..24530)
                     /locus_tag="ACHUPG_000339"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR0860102.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24551..24734)
                     /gene="ssrS"
                     /locus_tag="ACHUPG_000340"
     ncRNA           complement(24551..24734)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHUPG_000340"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="ACHUPG_000341"
     CDS             complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="ACHUPG_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR0860103.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25273..25851
                     /gene="ygfB"
                     /locus_tag="ACHUPG_000342"
     CDS             25273..25851
                     /gene="ygfB"
                     /locus_tag="ACHUPG_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR0860104.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25877..27202
                     /gene="pepP"
                     /locus_tag="ACHUPG_000343"
     CDS             25877..27202
                     /gene="pepP"
                     /locus_tag="ACHUPG_000343"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR0860105.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27199..28377
                     /gene="ubiH"
                     /locus_tag="ACHUPG_000344"
                     /gene_synonym="visB"
     CDS             27199..28377
                     /gene="ubiH"
                     /locus_tag="ACHUPG_000344"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR0860106.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28400..29602
                     /gene="ubiI"
                     /locus_tag="ACHUPG_000345"
                     /gene_synonym="visC"
     CDS             28400..29602
                     /gene="ubiI"
                     /locus_tag="ACHUPG_000345"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR0860107.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29676..29759)
                     /locus_tag="ACHUPG_000346"
     CDS             complement(29676..29759)
                     /locus_tag="ACHUPG_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0860108.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30050..31144
                     /gene="gcvT"
                     /locus_tag="ACHUPG_000347"
     CDS             30050..31144
                     /gene="gcvT"
                     /locus_tag="ACHUPG_000347"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR0860109.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31168..31557
                     /gene="gcvH"
                     /locus_tag="ACHUPG_000348"
     CDS             31168..31557
                     /gene="gcvH"
                     /locus_tag="ACHUPG_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR0860110.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31676..34549
                     /gene="gcvP"
                     /locus_tag="ACHUPG_000349"
     CDS             31676..34549
                     /gene="gcvP"
                     /locus_tag="ACHUPG_000349"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR0860111.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34816..35559
                     /locus_tag="ACHUPG_000350"
     CDS             34816..35559
                     /locus_tag="ACHUPG_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0860112.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35616..37049)
                     /gene="bglA"
                     /locus_tag="ACHUPG_000351"
     CDS             complement(35616..37049)
                     /gene="bglA"
                     /locus_tag="ACHUPG_000351"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR0860113.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37094..37404
                     /gene="yqfB"
                     /locus_tag="ACHUPG_000352"
                     /pseudo
     CDS             37094..37404
                     /gene="yqfB"
                     /locus_tag="ACHUPG_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
     gene            37568..38227
                     /locus_tag="ACHUPG_000353"
     CDS             37568..38227
                     /locus_tag="ACHUPG_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR0860114.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38423..39403)
                     /gene="ygfZ"
                     /locus_tag="ACHUPG_000354"
     CDS             complement(38423..39403)
                     /gene="ygfZ"
                     /locus_tag="ACHUPG_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR0860115.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39646..39912
                     /gene="sdhE"
                     /locus_tag="ACHUPG_000355"
     CDS             39646..39912
                     /gene="sdhE"
                     /locus_tag="ACHUPG_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR0860116.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39893..40300
                     /locus_tag="ACHUPG_000356"
     CDS             39893..40300
                     /locus_tag="ACHUPG_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR0860117.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40340..40861)
                     /gene="fldB"
                     /locus_tag="ACHUPG_000357"
     CDS             complement(40340..40861)
                     /gene="fldB"
                     /locus_tag="ACHUPG_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR0860118.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40973..41869
                     /gene="xerD"
                     /locus_tag="ACHUPG_000358"
     CDS             40973..41869
                     /gene="xerD"
                     /locus_tag="ACHUPG_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR0860119.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41894..42604
                     /gene="dsbC"
                     /locus_tag="ACHUPG_000359"
     CDS             41894..42604
                     /gene="dsbC"
                     /locus_tag="ACHUPG_000359"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR0860120.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42610..44343
                     /gene="recJ"
                     /locus_tag="ACHUPG_000360"
     CDS             42610..44343
                     /gene="recJ"
                     /locus_tag="ACHUPG_000360"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR0860121.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44434..45532
                     /gene="prfB"
                     /locus_tag="ACHUPG_000361"
     CDS             join(44434..44508,44510..45532)
                     /gene="prfB"
                     /locus_tag="ACHUPG_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR0860122.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45542..47059
                     /gene="lysS"
                     /locus_tag="ACHUPG_000362"
     CDS             45542..47059
                     /gene="lysS"
                     /locus_tag="ACHUPG_000362"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR0860123.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47102..47650)
                     /gene="idi"
                     /locus_tag="ACHUPG_000363"
     CDS             complement(47102..47650)
                     /gene="idi"
                     /locus_tag="ACHUPG_000363"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR0860124.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47705..47776
                     /locus_tag="ACHUPG_000364"
     CDS             47705..47776
                     /locus_tag="ACHUPG_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0860125.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47773..47898)
                     /gene="yqfG"
                     /locus_tag="ACHUPG_000365"
     CDS             complement(47773..47898)
                     /gene="yqfG"
                     /locus_tag="ACHUPG_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR0860126.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47900..49348)
                     /gene="uacT"
                     /locus_tag="ACHUPG_000366"
     CDS             complement(47900..49348)
                     /gene="uacT"
                     /locus_tag="ACHUPG_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFR0860127.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        12051 a        12449 c        13406 g        11923 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa actgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcaggcgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggggctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agccgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ttatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggtgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgatcacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc taggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgagta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcacttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ctggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcgcattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgcgattg ggctttctcg tttctcgccg ttatgaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggcgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgtg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagct
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgcaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg actgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttgttgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgatgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt tttctacggc taggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggt ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tcaattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttatcc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttagacat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caggcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gtcaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcagtcacag gggaggagca atcctccctt acccttactc actaaattag
    23881 gttaaagaat caacgatgtc aatcagggcg atgcaggtgt atcgccctta ccactcccag
    23941 actttcgacg gtgtaaccac cgcaggaaga gggatatccc actcttcaac ggggagtttt
    24001 tccaccaact gacaatcatg cgcataaccc accggttgcg ttttatagtg ctgccagttt
    24061 tgtaaggtcc gatcataaaa accaccgccc attcccagac gctgaccgta ctcatcaaag
    24121 gcgaccagcg gtgtgatcag cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc
    24181 agttttggct catggatctt caacctgttc atcaccagtt cgctttgcgg atggtaattc
    24241 aggaacagca aattaccggc actaaaggga tgcaaaactg gaagatatac gcgcttaccg
    24301 gcgcgccaga gttgttctat tagcggctgg gtgtcgagtt caccatcaaa agagaggaat
    24361 acagcgaccg tatgcgccat caccaccggg ggataagtca tcatccgggt agcggcttgt
    24421 tgacccattt ccagctgttg ttccggcgtt aacgcacgac gacgttgccg aatcattttg
    24481 cggatttctt gtcgggataa tgtcagtggg agctctggta gttgcgtcat ggctggtgcc
    24541 agataagaag ggaatctccg agatgccgcc gcaggctgta acccttgaac ccttggttca
    24601 aggtgaatgt gtcgtcgcag ttttaaggct tctcggacga accgagcatg ctcaccaacc
    24661 gcggagcgcc acattcttgt ggtatgaaat atcggctcag gggactggcc cgcttgcgaa
    24721 catctcagag aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt
    24781 caaagttttg gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg
    24841 tctgctgcag catccgaata cgctgttcca tacttgccgc gtagtcacga gtctttgctt
    24901 tttcttgcgc taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat
    24961 ttgtgactct agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgctgcct
    25021 gattcaacgc atccctttgg tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt
    25081 ggatatcgac gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt
    25141 ctttagaccc atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag
    25201 cccttttctg gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga
    25261 cgacgaatgc ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat
    25321 ctgaaccaac aagggacggg tctgacccct gccgagatgc atggtttaat cagtgggatg
    25381 atatgtggcg gtaacgatga cagctcatgg ctgccgctac ttcacgacct gacgaacgaa
    25441 ggcatggctt tcggtcatga gctggcacag gcactgcgta aaatgcactc tgccaccagc
    25501 gatgccctgc aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc
    25561 agcgttttcg atcgggctga tgcactggca ggttgggtca atcacttcct gcttggtctt
    25621 ggcgttacgc aaccgaagct ggacaaagtg accggcgaaa ccggtgaagc catcgacgat
    25681 ctgcgtaaca tcgcgcagct gggctacgac gaagacgaag atcaggaaga gcttgaaatg
    25741 tcgcttgaag agatcatcga atacgttcgt gttgccgcgc tgttatgcca cgacaccttt
    25801 actcatccgc aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa
    25861 acgtaaggag agtgttatga gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc
    25921 cctggtggag caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac
    25981 acgtagcgcc gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg
    26041 ctttaacgaa ccggaagcgg tgctggtgct gattaaaagc gatgacactc ataaccacag
    26101 cgttctgttt aaccgcgttc gcgacctgac ggcggagatc tggtttggcc gtcgcttagg
    26161 ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa
    26221 tcagcaactt tatcaactac ttaacggcct ggatgtggtt taccatgccc agggcgaata
    26281 tgcatatgct gatgaaatcg tgaacagtgc gctggaaaaa ctgcgtaaag gttcgcggca
    26341 aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct
    26401 gttcaaatcg ccagaagaga ttgccgtact ccgccgcgcg ggagaaatca ccgccatggc
    26461 acatacacgg gcgatggaaa aatgccgtcc gggaatgttc gagtaccatc tggaaggcga
    26521 aattcaccac gaatttaacc gccacggtgc gcgctatcct tcctataaca ccattgtagg
    26581 cagcggtgaa aacggctgca ttctgcacta caccgaaaac gagagtgaac tgcgcgacgg
    26641 tgacctggtg ttgattgacg cgggttgtga atacaaaggt tacgcgggcg atattacccg
    26701 caccttcccg gtcaacggca aattcaccca ggcccagcgt gaaatctacg acattgtgct
    26761 ggagtctctc gaaaccagcc tgcgcctgta tcgtccggga acttccattc tggaagtcac
    26821 tggtgaagtg gtgcgcatca tggttagcgg cctggtaaaa ctcggcatcc tgaaaggtaa
    26881 tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca
    26941 ctggttagga ctggatgtcc atgacgttgg cgtttatggt caggatcgct cgcgcattct
    27001 ggaaccgggg atggtactga ccgtagagcc tggtctgtat atcgcgccgg atgcggatgt
    27061 gccagaacaa tatcgcggta tcggcattcg tattgaagac gacattgtga ttaccgaaac
    27121 cggcaacgaa aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat
    27181 ggctgctgcg agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg
    27241 ctggcgctgg ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg
    27301 actgcgccag agtcacatgc tcatccgggc tttgatggac gagcgatagc gctggcggcg
    27361 ggtacctgtc agcaactggc gcgcatcggc gtctggcaat ctctggctga ttgcgcaacc
    27421 gccatcacta ccgtgcatgt cagcgatcgt ggtcatgctg gattcgtcac cctcgccgca
    27481 gaagattacc aactggcggc gctggggcag gttgtcgaat tgcataatgt cgggcaacgg
    27541 ctgtttgcat tgctgcgaaa agcacctggc gtaatgctgc attgccccga tcgcgtagct
    27601 aacgttgccc gtactcagag tcatgttgaa gtgacgctgg agagcggcga gatgctgacg
    27661 ggccgcgtgc tgatagccgc tgatggcacc cattcagcgt tagccaccgc gtgcggcgtt
    27721 gattggcagc aggagcctta cgaacaactg gcagtgattg ccaacgttgc tacttctgtt
    27781 gcgcacgaag gccgcgcttt tgaacgcttc acgcaacatg gcccgctggc gatgttgccg
    27841 atgtctgacg gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggtg
    27901 ctgtcgtgga gcgacgagaa gttttgccgt gaactccagt ctgcctttgg ctggcgactg
    27961 gggcaaatta cccacgccgg taaacgcagt gcttatccgc tggcattaac ccgcgccgcc
    28021 agaccgatta cccatcgcac tgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt
    28081 gccgggcaag ggtttaacct cggtatgcga gatgtcatga gccttgcgga aaccctgact
    28141 caggcgcatg agcgcggaga agacatcggt gattacggca tattgtgccg ctatcagcag
    28201 cgtcgacaga gcgatcgcga agcaaccatc ggcgtcacgg acagcctggt acatcttttt
    28261 gccaaccgtt gggcaccgct ggttgtcggg cgcaacatcg gactgatgac gatggaatta
    28321 ttcaccccgg cacgcgatgt gctggcgcag cgcaccctcg gttgggtggc gcgttgaagg
    28381 cggttttaag gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt
    28441 ggggctggcg gttgcctgcg gcttacaggg gagcggctta cgcgttgccg tactggagca
    28501 gcgcgtaccg gaacctctgg cggtagatgc actaccacaa ctgcgcgttt cggctatcaa
    28561 tgccgccagc gaaaaattac tcacccgtct tggcgtctgg caggacattc tcgctcgtag
    28621 ggccagttgt tatcacggca tggaagtgtg ggacaaagac agttttggtc acatctcgtt
    28681 tgacgatcaa agcatgggct atagccatct tgggcacatc gttgaaaact cagtgattca
    28741 ctacgcgctg tggaacaaag cgcagcagtc gtcagatatt actctgttgg cccccacaga
    28801 attacagcag gtcgcctggg gagaaaatga aaccttcctg acgctgaaag atggcagcat
    28861 gttaacggcg cgtctggtga ttggcgcgga cggtgctaat tcctggttgc gcaacaaagc
    28921 agatattccg ctgactttct gggattatca gcatcacgcg ctggtagcga ccattcgcac
    28981 ggaagaaccg catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt
    29041 tttaccgctt agcgatccgc atctttgctc gattgtctgg tcactgtcgc cagaggaagc
    29101 gcagcggatg cagcaggcaa gtgaagacga atttaatcgc gcgttaaata tcgcttttga
    29161 taatcgcctt ggcttatgca aggttgagag cgcgcgtcag gtgttcccgc tgacggggcg
    29221 ttatgcgcgc cagtttgccg cacaccgtct ggcgctggtg ggcgacgccg cgcataccat
    29281 tcacccactg gcggggcagg gcgttaatct cggatttatg gacgccgcag agctgattgc
    29341 tgaactgaaa cggttgcatc gtcagggtaa agacatcggg cagtacattt acctgcgccg
    29401 ctatgaacgt agccgcaagc acagcgcggc gctgatgctg gctggtatgc agggttttcg
    29461 cgatctcttt tccggcgcta atccggcgaa aaaactgctg cgtgatattg gtttgaaact
    29521 ggccgacacg cttcctggcg ttaagccgca acttatccgc caggcaatgg gattaaacga
    29581 tttgcctgaa tggctgcgtt aaaaatttct cctctgttgt ttatttgata cccatcacac
    29641 tttcatctcc cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc
    29701 tccgttttcg cattatattt tctaatacca tgattttttg atttagtgtt ttttgacatt
    29761 tttttagctc ttaatattgt cttattcaaa ttgactttct catcacatca tctttgtata
    29821 gaaattggtg tattttttgg ttttttattc tgtcgcgatt tttgcatttt ttaaccataa
    29881 gctaatgtga tgatcaattt taccttatgg ttaacagtct gtttcggtgg taagttcagg
    29941 caaaagagaa cgattgcgtt ggggaccggg agtggctccg atgctgggtt ttgtggtgat
    30001 aatttcacca tgaaaaagtt gtcagccccg cttattcaat gaggacaaga tggcacaaca
    30061 gactcctttg tacgaacaac acacgctttg cggcgctcgc atggtggatt tccacggctg
    30121 gatgatgccg ctgcattacg gttcgcaaat cgacgaacat catgcggtac gtaccgatgc
    30181 cggaatgttt gatgtgtcgc atatgaccat cgtcgatctc cgcggcagcc gcacccggga
    30241 gtttctgcgt tatctgctgg cgaacgatgt ggcgaagctc accaaaagcg gcaaagccct
    30301 ttactcgggg atgttgaatg cctctggcgg tgtgatagat gatctcatcg tctactactt
    30361 tactgaagat ttcttccgcc tcgttgttaa ctccgccacc cgcgaaaaag acctctcctg
    30421 gattacccaa cacgctgaac ctttcggcat cgaaattacc gttcgtgatg acctttccat
    30481 gattgccgtg caagggccga atgctcaggc aaaagctgcc acactgttta atgatgctca
    30541 gcgtcaggcg gtggaaggga tgaaaccgtt ctttggcgtg caggtgggcg atctgtttat
    30601 tgccaccact ggttataccg gtgaagcggg ctatgaaatt gcgctgccca atgaaaaagc
    30661 ggccgatttc tggcgtgcgc tggtggaagc gggcgttaag ccctgcggtc tgggggcgcg
    30721 tgacacgctg cgtctggaag cgggcatgaa tctttatggt caggagatgg acgaaactat
    30781 ttctccttta gccgccaaca tgggctggac tatcgcctgg gaaccgacag atcgtgactt
    30841 tatcggtcgt gaagccctgg aagtgcagcg tgagcatggt acagaaaaac tggttggtct
    30901 ggtgatgacc gaaaaaggcg tgctgcgtaa tgaactgccg gtacgcttta ccgatgcgca
    30961 gggcaaccag catgaaggca ttatcaccag cggtactttc tccccgacgc tgggttacag
    31021 cattgcgctg gcgcgcgtgc cggaaggtat tggcgaaacg gcgattgtgc aaattcgcaa
    31081 ccgtgaaatg ccggttaaag tgacaaaacc tgtttttgtg cgtaacggca aagccgtcgc
    31141 gtgatttact tttttggaga ttgattgatg agcaacgtac cagcagaact gaaatacagc
    31201 aaagaacacg aatggctgcg taaagaagcc gacggcactt acaccgttgg tattaccgaa
    31261 catgctcagg agctgttagg cgatatggtg tttgttgacc tgccggaagt gggcgcaacg
    31321 gttagcgcgg gcgatgactg cgcggttgcc gaatcggtaa aagcggcgtc agacatttat
    31381 gcgccagtaa gcggtgaaat cgtggcggta aacgacgcac tgagcgattc cccggaactg
    31441 gtgaacagcg aaccgtatgc aggcggctgg atctttaaaa tcaaagccag cgatgaaagc
    31501 gaactggaat cactgctgga tgcgaccgca tacgaagcat tgttagaaga tgagtaacgg
    31561 ctttattcct cttctgcggg agaggatcag ggtgaggaaa atttatgcct caccctcact
    31621 ctcttcgtaa ggagagaggt tcacaattca ctgcacgttt caggaaccat cgctcatgac
    31681 acagacgtta agccagcttg aaaacagcgg cgcttttatt gaacgccata tcggaccgga
    31741 cgccgcgcaa cagcaagaaa tgctgaatgc cgttggtgca caatcgttaa acgcgctgac
    31801 cggccagatt gtgccgaaag atattcagct tgcgacacca ccgcaggttg gcgcaccggc
    31861 gaccgaatac gccgcactgg cagaactcaa ggctattgcc agtcgcaata aacgcttcac
    31921 gtcttacatc ggcatgggtt acaccgccgt gcagctaccg ccggttatcc tgcgtaacat
    31981 gctggaaaat ccgggctggt ataccgcgta cactccgtat cagcctgaag tctcccaggg
    32041 ccgccttgaa gcactgctca acttccagca ggtaacgctg gatttgactg gactggatat
    32101 ggcctctgct tctcttctgg acgaggccac cgctgccgcc gaagcaatgg cgatggcgaa
    32161 acgcgtcagc aaactgaaaa atgccaaccg cttcttcgtg gcttccaatg tgcatccgca
    32221 aacgctggat gtggtccgta ctcgtgctga aacctttggt tttgaagtga ttgtcgatga
    32281 cgcgcaaaaa gtgctcgacc atcaggacgt cttcggcgtg ctgttacagc aggtaggcac
    32341 taccggtgaa attcacgact acactgcgct tattagcgaa ctgaaatcac gcaaaattgt
    32401 ggtcagcgtt gccgccgata ttatggcgct ggtgctgtta actgcgccgg gtaaacaggg
    32461 cgcggatatt gtttttggtt cggcgcaacg cttcggcgtg ccgatgggct acggtggccc
    32521 acacgcggca ttctttgcgg cgaaagatga atacaaacgc tcaatgccgg gccgtattat
    32581 cggtgtatcg aaagatgcag ctggcaatac cgcgctgcgc atggcgatgc agactcgcga
    32641 gcaacatatt cgtcgtgaga aagcgaactc caacatttgt acttcccagg tactgctggc
    32701 aaacatcgcc agcctgtatg ccgtttatca cggcccggtt ggcctgaaac gtatcgctaa
    32761 ccgcattcac cgtctgaccg atattctggc ggcgggcctg caacaaaaag gtctgaaact
    32821 gcgccatgcg cactatttcg acaccttgtg tgtggaagtg gccgacaaag cgggcgtact
    32881 gacgcgtgcc gaagcggctg aaatcaacct gcgtagcgat atcctgaacg ccgtggggat
    32941 cacccttgat gaaaccacca cgcgtgaaaa cgtaatgcag cttttcagtg tgctgctggg
    33001 cgataaccac gggctggaga tcgacacgct ggacaaagac gtggctcacg acagccgctc
    33061 tatccagcct gcgatgctgc gcgacgacga aatcctcacc catccggtgt ttaatcgcta
    33121 tcacagcgaa accgaaatga tgcgctatat gcactcgctg gagcgtaaag atctggcgct
    33181 gaatcaggcg atgatcccgc tgggttcctg caccatgaaa ctgaacgccg ccgccgagat
    33241 gatcccaatc acttggacgg aatttgccga actgcacccg ttttgcccgc cggaacaggc
    33301 tgaaggttat cagcagatga ttgcgcagct ggctgactgg ctggtgaaac tgaccggtta
    33361 cgacgccgtt tgtatgcagc cgaactccgg cgcacagggc gaatacgcgg gcctgctggc
    33421 gattcgtcat tatcatgaaa gccgcaacga agggcatcgc gatatctgcc tgatcccggc
    33481 ttctgcgcac ggaactaacc ccgcttctgc acatatggca ggaatgcagg tggtggttgt
    33541 ggcgtgtgat aaaaacggca acatcgatct gactgatctg cgcgcgaaag cggaacaggc
    33601 gggcgataac ctctcctgta tcatggtgac ttatccttct acccacggcg tgtatgaaga
    33661 aacgatccgt gaagtgtgtg aagtcgtgca tcagttcggc ggtcaggttt accttgatgg
    33721 cgcgaacatg aacgcccagg ttggcatcac ctcgccgggc tttattggcg cggacgtttc
    33781 gcacctcaac ctgcataaaa ctttctgcat tccgcacggc ggtggtggtc cgggtatggg
    33841 accgatcggt gtgaaagcgc atctggcacc gtttgtaccg ggtcatagcg tggtgcaaat
    33901 cgaaggcatg ttaacccgtc agggcgcagt ttctgcggca ccgttcggta gcgcctctat
    33961 cctgccaatc agctggatgt acatccgcat gatgggcgca gaagggctga aaaaagcaag
    34021 ccaggtggca atcctcaacg ccaactatat tgccagccgc ctgcaggatg ccttcccggt
    34081 gctgtatacc ggtcgcgacg gtcgcgtggc gcacgaatgt attctcgata ttcgcccgct
    34141 gaaagaagaa accggcatca gcgagctgga cattgccaag cgcctgatcg actacggttt
    34201 ccacgcgccg acgatgtcgt tcccggtggc gggtacgctg atggttgaac cgactgaatc
    34261 tgaaagcaaa gtggaactgg atcgctttat cgacgcgatg ctggctatcc gcgcagaaat
    34321 tgaccaggtg aaagccggtg tctggccgct ggaagataac ccgctggtga acgcgccgca
    34381 cattcagagc gaactggtcg ccgagtgggc gcatccgtac agccgtgaag ttgcggtatt
    34441 cccggcaggc gtggcagaca aatactggcc gacggtgaaa cgtctggatg atgtttacgg
    34501 cgaccgtaac ctgttctgct cctgcgtacc gattagcgaa taccagtaat tcactgattc
    34561 gagtattttc taaaggcgct tcggcgcctt tttagtcaga tgacaaagta caaaagtgct
    34621 cagacagtcc cctcgcccct ttggggagag ggttagggtg aggggaacag gccggcactg
    34681 gcgcgaatat ttaccctcac cccgaccctc tccctgaaag ggcgaggggg aaaagcgtgc
    34741 caacattgaa gattgagcca gtttgttagc aatctcaaag atacgtcaac gaattaattt
    34801 ttctcggaaa aacaaatggc tatagcactt gtgaccggtg gcagtcgcgg catcgggcgg
    34861 gcaactgcat tactgttggc gcaagaaggg tatacggtgg cggttaatta tcagcaaaac
    34921 ctccacgcgg cgcaggaagt gatgaactta ataatgcaag ccggtggcaa agcattcgtg
    34981 ctccaggcgg atatcagcga cgaaaaccag gtcgttgcga tgtttacagc aatcgatcag
    35041 cacgatgaac cgctagcagc gctggtcaat aacgccggga tcttgtttac ccagtgcacc
    35101 gttgaaaacc ttaccgcaga gcgaatcaac cgagtacttt ccaccaacgt gacgggatat
    35161 tttctctgct gccgcgaggc ggtaaaacgc atggcgctta aaaatggtgg cagtggcggc
    35221 gctatcgtca atgtctcttc ggtggcctca cggttgggtt cgccagggga atatgttgat
    35281 tacgcggcat cgaaaggggc gattgattcg ttaaccaccg gactatcgct ggaagtcgcc
    35341 gcgcagggga tccgcgttaa ctgcgtgcgg ccagggttta tttataccga aatgcacgcc
    35401 agcggcggcg agcctggacg cgtcgatcgc gttaagtcga acatccccat gcagcgtggt
    35461 ggacaggcag aagaggtcgc gcaggccatt gtctggctac taagtgataa agcctcttac
    35521 gtcacgggaa gttttatcga tttggcgggc gggaaataaa acagggaagt tgtctgaccg
    35581 gatgcaacaa gtattgcatc cggtacttca tcgacttaaa gcttctcgcc gttgctggca
    35641 atcacctctt tgtaccagtt aaagctcttc ttacgtgaac gcgacatatc gccagtaccg
    35701 tcgtcatgtt tattcacata gataaagccg tagcgtttgc tgtactgccc ggtagtgaat
    35761 gacacgcagt cgatgcagcc ccacggtgtg tagcccatca gatccacacc atcgtaagtc
    35821 actgcttttt tcatctcttc aatatgggcg cgcaggtagt caatgcggta gtcgtcgttg
    35881 atgctgccat cttcttccac tttgtcgtaa gcgccaaaac cgttttcgac aataaacagc
    35941 ggcctctgat aacgctcata cagttcgcaa agggcatagc gcagacctac tggatcaatc
    36001 tgccagcccc agtcagacgc tttaacatac gggttcggta cgctgccttc aaaaccagag
    36061 atcgcatcgc cggtgccgcc ttcggccttc actgcgttgg tcatgtaata gctgaaacca
    36121 agataatcgc aggtgccttc gcgcagtaca tcgagatcgc cgtcttccat tttgatatta
    36181 aatccgcgac gttcccactc gttcaataca taggacgggt aatagccgca tagctgcaca
    36241 tcggtaaaga cgtagcgttc gcgcatcgac tcctgagcga acatcacatc gtccgggtta
    36301 catgagtagg gatagagcgg caccatcgcc agcatacagc cgactttcat ctccgggtta
    36361 atgcgacgcg cggctttcac tgccagggcg ctggcgacaa actggtgatg cagcacctgg
    36421 tacatcgtct cttccgggtt gtcatgctcg gtatacacca cgccggagca gcagtaacca
    36481 aacagcggcg cacgccagtt acgctggttg tttatttcgt tgaaggtcat ccagtatttg
    36541 actttatgct tgtagcgttc gaataccact tccgcgaaac ggacaaagaa atcaacgaat
    36601 ttacggttgg tccagctacc gtattgctgc accagatgca gcggcatttc aaagtgggag
    36661 agggttatca ccggttcgat gttgtatttc agcagttcat cgaacagaga gtcgtaaaac
    36721 ttcagccctt cttcgtttgg ctgagcttca tcaccttttg gaaaaatacg cgtccaggcg
    36781 atggaagtcc gaaaacattt gaagcccatt tcggcaaata gcttgatgtc ttccttgtag
    36841 tgaccataaa aatcaacggc ttcatggttt ggatagtatt ttcctggcaa gacttctttg
    36901 gtgatttcgc gcggcacgcc gtgtgcgcca caggtcagaa cgtcacaaat gctcggtcct
    36961 ttgccgcctt tgttccagcc gccttcgacc tgatgagcgg caacggcgcc accccataag
    37021 aaatctttcg gtaaggtgag ttttttcact atcataggct ccagatcagt caattcttac
    37081 aggaaatagt accatgcagc caaacgacat cacttttttc aacgtttcca ggatgacatt
    37141 ctggctgggc gtaaaaccat caccatccgc gacgagtctg aatcgcactt caaaacgggt
    37201 gatgtgcttc gtgtcggacg ttttgaagat gacggttatt tttgcacgat tgaagtcacc
    37261 gcaacctcaa ccgtaacgct ggatacactg acggaaaaac atgcagagca ggaaaatatg
    37321 accctgcctg aactgaaaaa ggtcattgct gacatctatc ccggtcagac acaattttat
    37381 atgattgaat ttaaatgtct ttaaattgaa ctcacgcctg ttagttgaaa tttcgcattt
    37441 aatttcatga tttttaggag attagtctca aatcagaatt tattttagct aacaggtgtt
    37501 cactggaact attctcagtt acgctggaga ggtatacatg tgcgtgtacg tttctctgga
    37561 gtaagttatg gttcagaagc ccctcattaa gcagggatat tcgctggcag aggaaattgc
    37621 caacagcgtc agtcacggca ttgggctggt gtttggtatc gttgggctgg tgttgctact
    37681 ggttcaggcg gtggatctca atgccagcgc cacagcgata accagttaca gcctctatgg
    37741 cggcagtatg atcctgctgt tcctcgcttc gacgctctat cacgccattc ctcatcaacg
    37801 ggcaaaaatg tggctgaaga aatttgacca ttgcgctatt tacctgttga ttgccggaac
    37861 ctacacgccg tttttgctgg tggggctgga ttctccgtta gcgcgcgggt tgatgattgt
    37921 tatctggagc ctagcattgc tggggattct gttcaaactg accatcgcgc accgattcaa
    37981 aattttatct ctggtgactt atctggcgat gggctggctg tcgctggtgg taatttatga
    38041 aatggcagtt aagctcgcgg cgggcagcgt taccttactg gcggtaggcg gcgtggttta
    38101 ttcgctcggg gtgattttct acgtctgcaa acgcattcca tacaaccatg ccatctggca
    38161 cggcttcgtg ctcggcggta gtgtgtgcca ctttctggcg atctatttgt atattgggca
    38221 ggcgtaatgg gtatgccttt gtaggcctga taagacgcgt cagcgtcgca tcaggcatcg
    38281 gagcgcaacc gccggatgcg gcgtgaacgc cttatccgat ctacgggcct cgtccctgtt
    38341 gtgataaagg catcaaatcc cagacatgat aagacgcgtc agcgtcgcat caggcgataa
    38401 atgcctgatg cgatacagag atttactctt cgagcgaata cggcagcggc tcgatatgca
    38461 gtgtattcgc atcgtcgcgt acgcggaaga tgctgtccgg ttccatatcg ttattcatga
    38521 cgacctgtac cacgacctga ccatcttcca gttttaccgc agccagcacc gtaccggtac
    38581 gacgccagtt ctcgcccatt ttcagctcta agtcttcacc agcttccggc agtcggctgg
    38641 cactacctgc cagcaaccag agcgcgcgtt tgttggcacc acggaatttt gctcgcgcca
    38701 ccatctcttg tccggtataa cagcctttct taaagctgat accgcccagc gcctggagat
    38761 tggtcgcctg tgggataaac tgcccgctgt tggcggcatc aatcaccggg aaaccagctt
    38821 caatgtttaa tgccagccac tgttggctat tgttcagttc cgcttcaccg cgcagtttat
    38881 cggtcagcat attagcagta gcttcatcgg ttacgatcag gaaacgttct gccgggtgtt
    38941 caaaccatag caaagtggtc gcgccttctt tgactacctg tttttctttc gaaggcagtt
    39001 cgctaaagag atttgccagc gcggcgcgcg cctgaaaacc ggcaacacca agcagcacac
    39061 gctcgtcgtc tggcgcgatg gtcactttag agaataccgc atattttttc agttcagtca
    39121 gctgcggttc acgcacgctg cgccgttcaa tccatgcaaa gccatcgccg tcgcggaaca
    39181 gacgtaaatt gctccacatt ttacctttgg cgtcgcaatg ggcggcgagc aggtgctgat
    39241 cttctgccat ctggctgaca tctgctgtca cctgaccctg catatatttt tcgctgtccg
    39301 cgccagtaat ggtggcaagc gcccagtcat caagcgtcat cagcgtcagc ggtaaacggg
    39361 cagaagccgt aggctgacgt ggaggaaaag gtgtaaaagc cataatagag tcctgagttg
    39421 cttaacggcg tattaatgac taatggtaaa agagctatga ggcaatgcaa gtgctttcac
    39481 tacgcgatct gtgccttctg cgaggcgact gccaaaaaaa tgaagccctt gaattgttgt
    39541 gaattaattt cgcgatcgcg atggtgttta ctgatattcc cgtaaaagaa gtcgaaaaac
    39601 acgttacaat agccgggtac tggattttcg tgagacagga agaagatgga cattaacaac
    39661 aaagcccgaa ttcattgggc atgccgccgt ggtatgcgcg aactcgatat ttcaatcatg
    39721 tcgtttttcg aacatgagta cgacagctta agcgatgacg aaaaacgcat ctttattcgt
    39781 ctgctggaat gtgacgatcc ggacctgttt aactggctga tgaatcacgg taaaccggcc
    39841 gatgcagaac tggaaataat ggtccgactc atccagacac ggaaccggga acgtggtcct
    39901 gtggcaatct gatttgcgcg tctcctggcg cgcacagtgg ctttccttgc tgattcatgg
    39961 gctggttgcc gctgttattt tactcatgcc ctggccgctc agttacaccc cgttatggat
    40021 ggtgttactt tcgctggtgg tatttgattg cgttcgcagc cagcggtgta ttaatgctcg
    40081 ccagggggaa attcgcttgt tgatggacgg gcgtttgcgt tggcaagggc aggagtggag
    40141 catcgtcaaa gcaccgtgga tgattaagag cggcatgatg ctgcgtttac gttctgatgg
    40201 cggtaaacgg caacatttat ggctggcagc cgacagcatg gacgaagccg aatggcggga
    40261 tttacggcgg attttgttgc aacaagagac gcaaagataa agatgacggc aaagcatatg
    40321 cgctaagccg tcagtgagat caggcgtaat gctctgccat ttcgttgagg atttgctcgc
    40381 accagctctg aatacgctcg tcgctaaggt catactggtt agtttcatcc agcgccagac
    40441 ccacgaacag ttgcccgtca gcaatcaccg gtttcgggct ggtaaattca tatccttccg
    40501 ttggccagta gccgacgaac ttcacgcctt tggtcgagag tttgtcatgc agcataccga
    40561 gcgcatcgag gaaccactcg ccgtatccca gttgatcgcc aagcccatac agcgcaataa
    40621 ttttaccttc aaggttcagg tcgtcgagct gatcccagac ggcttcccag tcttcctgga
    40681 tttcaccaaa atcccaggtc gggataccca gaatgagcac atcgtactgc tccattaatt
    40741 tcggggagtc gtccttgagg ttatgtaagg tcaccagttc tgggccgata atatcgcgga
    40801 ttttttctgc cgccatttcg gtgtaacagg tgctggaacc gtaaaaaaga cccatattca
    40861 taacgtaagc ttctcgattc tgctgtggct tttgcagcta gtgtatcaga atcgctttag
    40921 tcacggcaca ataaggcata atggatcaaa tgagtgacca taaggggcgc aagtgaaaca
    40981 ggagctggca cgcatcgagc agtttcttga tgctctgtgg ctggaaaaaa atctggctga
    41041 aaatacgttg aacgcttacc gtcgcgatct gtcaatgatg gtggagtggt tgcatcaccg
    41101 cgggttgacg ctggcgacgg cgcaaagtga cgatttgcag gcattactgg cagaacggct
    41161 ggagggcggg tataaagcca ccagctcagc gcgtttgctg agtgcagtgc gccgattgtt
    41221 ccagtatctt tatcgcgaaa agtttcgtga agacgatccc agtgcgcatc tcgcttcacc
    41281 gaaattgccc cagcgtttgc caaaagattt aagcgaagcg caggtcgaac gtttattaca
    41341 ggcgccatta attgatcagc cactggagct acgcgataaa gccatgcttg aagtgttgta
    41401 tgctaccggg ctgcgtgtct ctgaactggt cgggctgaca atgagtgata tcagcctgcg
    41461 tcagggcgta gtgcgggtca ttggtaaagg caacaaagag cgcctggtgc cgttagggga
    41521 agaggcggtt tactggctgg aaacctatct ggaacatggg cgtccgtggc tgctgaatgg
    41581 tgtgtcaatt gacgtgttgt ttcccagcca gcgtgcgcag cagatgacgc gacagacctt
    41641 ctggcaccgc attaaacatt atgcagtgct ggcgggtatc gacagcgaaa agctgtcacc
    41701 gcatgtgttg cgtcacgctt ttgccactca tttattaaat catggtgcgg atttacgcgt
    41761 ggtgcagatg ctactgggcc acagcgatct ttccaccacg caaatttata cgcatgtcgc
    41821 taccgagcgt ctgcggcaac ttcatcaaca gcatcacccg cgggcgtgat gtctgaaaag
    41881 aacgggaaga tttatgaaga aaggttttat gttgtttact ttgttagcgg cgttttcagg
    41941 ctttgctcag gctgatgacg cggcaattca acaaacgtta gccaaaatgg gcatcaaaag
    42001 cagcgatatt cagcccgcgc ccgtagctgg catgaagaca gttctgacta acagcggtgt
    42061 gttgtacatc accgatgatg gtaaacatat cattcagggg ccaatgtatg acgttagtgg
    42121 cacggctccg gtcaatgtca ccaataagat gctgttaaag cagttgaatg cgcttgaaaa
    42181 agagatgatc gtttataaag cgccgcagga aaaacacgtc atcaccgtgt ttactgatat
    42241 tacctgtggt tactgccaca aactgcatga gcaaatggca gactataacg cgctggggat
    42301 caccgtgcgt tatcttgctt tcccgcgcca ggggctggac agcgatgcag agaaagaaat
    42361 gaaagctatc tggtgtgcga aagataaaaa caaagcgttt gatgatgtga tggcaggtaa
    42421 aagcgtcgca ccagccagct gcgacgtgga tattgccgac cattacgcac ttggcgtcca
    42481 gcttggcgtt agcggtactc cggcagttgt gctgagcaat ggcacacttg ttccgggtta
    42541 ccagccgccg aaagacatga aagaattcct cgacgaacac caaaaaatga ccagcggtaa
    42601 ataattcgcg tgaaacaaca gatacaactt cgtcgccgtg aagtcgatga aacggcagac
    42661 ttgcccgctg aattgcctcc cttgctgcgc cgtttatacg ccagccgggg agtacgcagt
    42721 gcgcaagaac tggaacgcag tgttaaaggt atgttgccct ggcagcaact aagcggtgtc
    42781 gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag gaacgcggat tattgtggtc
    42841 ggtgatttcg acgccgacgg cgcgaccagc acggctctaa gcgtgctggc gatgcgctcg
    42901 cttggttgca gcaatatcga ctacctggta ccaaaccgtt tcgaagacgg ttacggctta
    42961 agcccggaag tggtcgatca ggcccatgcc cgtggcgcgc agttaattgt cacggtggat
    43021 aacggtattt cctcccatgc gggagttgag cacgctcgct cgttgggcat cccggttatt
    43081 gttaccgatc accatttgcc gggcgacaca ttacccgcag cggaagcgat cattaaccct
    43141 aacttgcgcg actgtaattt cccgtcgaaa tcactggcag gcgtgggtgt ggcgttttat
    43201 ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct ggtttgatga gcgcggcatc
    43261 gcaattccta acctggcaga actgctggat ctggtagcac tggggacagt agcggacgtc
    43321 gtgccgctgg acgctaataa tcgcattctg acctggcagg ggatgagtcg catccgtgcc
    43381 ggaaagtgcc gtccagggat taaagcgctg ctggaagtgg caaatcgcga tgcacaaaaa
    43441 ctagccgcca gcgatttagg ttttgcgctg ggaccgcgtc tcaatgctgc cgggcgactg
    43501 gatgatatgt ccgttggtgt ggcgctgttg ctgtgcgaca acatcggcga agcgcgcgtg
    43561 ctggcaaatg aactcgatgc gctaaaccag acgcgaaaag agatcgaaca aggaatgcag
    43621 gttgaagccc tgaccctgtg cgagaaactg gagcgcagcc gtgacacgct acccggcggg
    43681 ctggcaatgt atcaccccga atggcatcag ggcgttgtcg gtattctggc ttcgcgtatc
    43741 aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag caggtgacgg tacgctgaaa
    43801 ggttccggtc gctccattca ggggctgcat atgcgtgatg cgctggagcg attagacaca
    43861 ctctaccccg gcatgatgct caagtttggc ggtcatgcga tggcggcggg tttgtcgctg
    43921 gaagaggata aattcgaact ctttcaacaa cggtttggcg aactggttac tgagtggctg
    43981 gccccttcgc tattgcaagg cgaagtggtg tcagacggtc cgttaagccc ggccgaaatg
    44041 accatggaag tggcgcagct gctgcgcgat gctggcccgt gggggcagat gttcccggag
    44101 ccgctgtttg acggtcattt ccgtctgctg caacagcggc tggtgggcga acgtcatttg
    44161 aaggtgatgg tcgaaccggt cggcggcggt ccactgctgg atggtattgc ttttaatgtc
    44221 gataccgctc tctggccgga taacggcgtg cgcgaagtgc aactggctta taagctcgat
    44281 atcaacgagt ttcgcggcaa ccgcagcctg caaattatca tcgacaatat ctggccaatt
    44341 tagcgtcatc ttctctataa aaaagagcgt ggattgggta caatcccgct cttatcaccg
    44401 cattttgact agctcaataa aagaaatcag accatgtttg aaattaatcc ggtaaataat
    44461 cgcattcagg acctcacgga acgctccgac gttcttaggg ggtatctttg actacgacgc
    44521 caagaaagag cgtctggaag aagtaaacgc cgagctggaa cagccggatg tctggaacga
    44581 acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc ctcgaagccg ttgtcgacac
    44641 cctcgaccaa atgaaacagg ggctggaaga tgtttctggt ctgctggaac tggctgtaga
    44701 agctgacgac gaagaaacct ttaacgaagc cgttgctgaa ctcgacgccc tggaagaaaa
    44761 actggcgcag cttgagttcc gccgtatgtt ctctggcgaa tatgacagcg ccgactgtta
    44821 cctcgatatt caggcggggt ctggcggtac ggaagcacag gactgggcga gcatgcttga
    44881 gcgtatgtat ctgcgctggg cagaatcgcg tggtttcaaa actgaaatca tcgaagagtc
    44941 ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa atctccggcg attacgctta
    45001 cggctggctg cgtacagaaa ctggcgttca ccgcctggtg cgtaaaagcc cgtttgactc
    45061 cggcggtcgt cgccacacgt cgttcagctc tgcgtttgtt tatccggaag ttgatgatga
    45121 tattgatatc gaaatcaacc cggcggatct gcgcattgac gtttatcgcg cgtccggcgc
    45181 gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt attacccaca tcccgaccgg
    45241 gatcgtgacc cagtgccaga acgaccgttc ccagcacaag aacaaagatc aggccatgaa
    45301 gcagatgaaa gcgaagcttt atgaactgga gatgcagaag aaaaatgccg agaaacaggc
    45361 gatggaagat aacaaatctg acatcggctg gggcagccag attcgttctt atgtccttga
    45421 tgactcccgc attaaagatc tgcgcaccgg ggtagaaacc cgcaacacgc aggccgtgct
    45481 ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa gcagggttat gaggaaccaa
    45541 catgtctgaa caacacgcac agggcgctga cgcggtagtc gatcttaaca atgaactgaa
    45601 aacgcgtcgt gagaagctgg cgaacctgcg tgagcaggga attgccttcc cgaacgattt
    45661 ccgtcgcgat catacctctg accaattgca cgcagaattc gacggtaaag agaacgaaga
    45721 actggaagcg ctgaacatcg aagtcgccgt tgctggccgc atgatgaccc gccgtattat
    45781 gggtaaagcg tctttcgtta ccctgcagga tgtgggtggc cgcattcagc tgtacgttgc
    45841 ccgtgacgat ctgccggaag gcgtttacaa cgagcagttc aaaaaatggg acctcggcga
    45901 catcctcggc gcgaaaggta aactgttcaa aaccaaaacc ggcgaactgt ctatccactg
    45961 taccgagctg cgtctgctga ccaaagcact gcgtccgctg ccggataaat tccacggctt
    46021 gcaggatcag gaagcgcgct atcgtcagcg ttatctggat ctcatctcta acgatgaatc
    46081 ccgtaacacc tttaaagtac gctcgcagat cctctctggt attcgccagt tcatggtgaa
    46141 ccgcggcttt atggaagttg aaacgccgat gatgcaggtg atcccaggcg gtgccgctgc
    46201 gcgtccgttt atcacccacc ataacgcgct ggatctcgac atgtacctgc gtatcgcgcc
    46261 ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag cgtgtattcg aaatcaaccg
    46321 taacttccgt aacgaaggta tttccgtacg tcataaccca gagttcacca tgatggaact
    46381 ctacatggct tacgcagatt acaaagatct gatcgagctg accgaatcgc tgttccgtac
    46441 tctggcacag gatattctcg gtaagacgga agtgacctac ggcgacgtga cgctggactt
    46501 cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc aagaaatatc gcccggaaac
    46561 cgacatggcg gatctggata acttcgactc tgcgaaagcg attgctgaat ctatcggtat
    46621 ccacgttgag aagagctggg gtctgggccg tatcgttacc gagatcttcg aagaagtggc
    46681 agaagcacat ctgattcagc cgaccttcat tactgaatat ccggcagaag tttctccgct
    46741 ggcgcgtcgt aacgacatta acccggaaat cacagaccgc tttgagttct tcatcggtgg
    46801 tcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg gaagatcagg cgcaacgctt
    46861 cctggatcag gttgccgcga aagacgcagg tgacgacgaa gcgatgttct acgatgaaga
    46921 ttacgttacc gcactggaac atggcttacc gccgacagca ggtctgggga ttggcatcga
    46981 ccgtatggta atgctgttca ccaacagcca taccatccgc gacgttattc tgttcccggc
    47041 gatgcgtccg gtgaaataag cattacgtta tgctcacaac cccggcaaat gtcggggttt
    47101 tttatttaag ctgggtaaat gcagataatc gtattctggc ttcgcgattt gtcgcctgca
    47161 tcaccatcca cggactgaac gcccacggcg tggcatcaat accgcgtaat acatcagcta
    47221 aatcacacca ttgataatcc atcacttcat catcgttgat ctgtaacgca ctggtggtgc
    47281 gtgcggcaaa taccggacac acttcatttt ccacaatgcc tctcggatcg gtggcgcggt
    47341 agcgaaagtc aggatagata gattcaggag gcgtaatttc cacgccaagc tcataacggc
    47401 aacggcggat caccgcgtct tcgctgcttt ctcccagttg tgggtgccca caaaccgagt
    47461 tagtccacac gccaggccac gcttttttgc tcagtgcgcg gcgggtaact aataattgcc
    47521 ccttggcatt aaacagccaa ctggagaacg cgagatgtaa gagggtgtct gccgtgtgtg
    47581 cggcatactt ttccagcgta cccgtgggaa ctccctgtgc attcaataaa atgacgtgtt
    47641 ccgtttgcat aatttctcac atgtaattct gatcactcat cattatagat aattgaagag
    47701 tgaaatgatt aaccaagaga gcgtttatcg acaaccgccc gttttgagcg gatgccgaca
    47761 ggtaaaaacc atttaaaaaa gtagcatcat atatttgaag cgactttcaa tggccattgc
    47821 aacacagtca gaaatgacag gaatagagag agtaaaaagc aacaacagac tgaatatagc
    47881 gcgcattaaa aaattcatat tattctccat gctcattttt cttcagcttg cgcatcagaa
    47941 gccacatacg tactgtcctg acttctaaat ctttatcaga gacggattcc ttcaccaggt
    48001 cagcatgatg ctgatagcca ttaaagaaga cgttcagcac aacggcgcta agggttgcga
    48061 gcataatgcc gctatgtagc agcggttgca gtacggccgg taacttagaa aagaaatcgt
    48121 gagagagcgt cggagtcatg ccaacgccga gactgatcgc cacaatatag aggttgtaac
    48181 ggttggtggt gtagttacag cgcgacagaa ttcgaatccc tgtcgccagt accatgccga
    48241 acatcactag accagcgccg cccagcacaa attgcggaat ggaggctacc agcaccgcca
    48301 tttttggcac catgccaaac aggattaata taattcccga agaaatacac acccagcggc
    48361 tatgaacgcg catcacgcta accaggccaa cgttttgaga aaatgacgtg tgggggaagc
    48421 tgttaaacgt gccgcctatc attgtcccta cgccatcgac acgcagcccg caaataatat
    48481 cgtgtgaaga gagtttacga ccgactattt cacccagtgc caggaacatc cccatcgact
    48541 cgataaacac gatgattaac acggcagtca tggtcagaat ggaaacggga tcgaaaatcg
    48601 gcataccaaa cgacatcggg gtgacaatcg caaaccacga ggcatcatgt agcccggata
    48661 aattgacttc attcatcatc cacgaaagta aaaagccaaa tacaatcccc agtaatacgg
    48721 cgacgttgga cataaatcct ttcgcatagc gagtaatgag caagataaaa attaagacgg
    48781 caaaggagat acctaaataa acgggattac catattgcgg attcccttta cctccagcgg
    48841 cccagtcaat acccacctga atgatgctaa gcccaataga agtaataacc acaccggtaa
    48901 ccagtggcgg gaataaaggc atcaagcgac caataagtgg cgctaataat gtggtgataa
    48961 aacccgcggc gatagtggca ccaaatatcc ccagcaggcc gatatccggg ttcatcccaa
    49021 tggctatcat tggtgttaca gcagcaaagg tcaccgacat aatcaccggc aggcggatcc
    49081 ccataaagcg gccgataccg atacattgca ataatgtgac gatcccgcag caaaagagat
    49141 ccgagctaat gagcatcgca atagcatctt ttgagaggcc cagtcggtca ccaatcataa
    49201 gaggaacagc gactgcacct gcgtacatga caaggacgtg ttgcagaccg agtatgatca
    49261 gctttcctgg tgataatatg cggtcaacct catcagttgg gcggtcttgc ccagaagatg
    49321 agggaagttg ggaatctatg gcgctcatca gtgtttctcc tttcgcccgc cagaataaaa
    49381 agcaaccgtt tacgttaatg aaactgtcta aacacatgct cgtggagtgc cagtttttgc
    49441 gaggaagggg agttagagac aggaattgcg gaggcaatca cataatcgtg cttaaaccac
    49501 gaaaatgaca agagaaactg acaatggaat tatctgtaat tttctcatat tgatatttca
    49561 ttaatcattc tggcatgtcg cactctcgca tttaatcgtt tttatctgga tagcgctctt
    49621 ttgatcggca atgttgaaat aatgaaagcg gcatagattg aggggcaata atttgaactc
    49681 agtcgcaaaa ttataattaa aacggtcatg ctacctaata tatgcccgtt tattgcatcg
    49741 cgaaatttga gaagacataa ctcattaaat gaaagggatg caaatgaata cgtttatcgc
    49801 tgctgaagct gcggaatgta taggctgtc
//
LOCUS       DAZPAB010000007        48784 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZPAB010000007 DAZPAB010000000
VERSION     DAZPAB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.007"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            380..1597
                     /gene="alaA"
                     /locus_tag="ACHUPG_000367"
     CDS             380..1597
                     /gene="alaA"
                     /locus_tag="ACHUPG_000367"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR0860128.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1681..2280
                     /gene="yfbR"
                     /locus_tag="ACHUPG_000368"
     CDS             1681..2280
                     /gene="yfbR"
                     /locus_tag="ACHUPG_000368"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR0860129.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2339..4171)
                     /locus_tag="ACHUPG_000369"
     CDS             complement(2339..4171)
                     /locus_tag="ACHUPG_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR0860130.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4258..4908)
                     /gene="hxpA"
                     /locus_tag="ACHUPG_000370"
     CDS             complement(4258..4908)
                     /gene="hxpA"
                     /locus_tag="ACHUPG_000370"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFR0860131.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4919..5413)
                     /locus_tag="ACHUPG_000371"
     CDS             complement(4919..5413)
                     /locus_tag="ACHUPG_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR0860132.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5496..5951)
                     /gene="yfbV"
                     /locus_tag="ACHUPG_000372"
     CDS             complement(5496..5951)
                     /gene="yfbV"
                     /locus_tag="ACHUPG_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR0860133.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6289..7491
                     /gene="ackA"
                     /locus_tag="ACHUPG_000373"
     CDS             6289..7491
                     /gene="ackA"
                     /locus_tag="ACHUPG_000373"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR0860134.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7566..9710
                     /gene="pta"
                     /locus_tag="ACHUPG_000374"
     CDS             7566..9710
                     /gene="pta"
                     /locus_tag="ACHUPG_000374"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR0860135.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9900..11420
                     /gene="yfcC"
                     /locus_tag="ACHUPG_000375"
     CDS             9900..11420
                     /gene="yfcC"
                     /locus_tag="ACHUPG_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR0860136.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11453..11995)
                     /gene="yfcD"
                     /locus_tag="ACHUPG_000376"
     CDS             complement(11453..11995)
                     /gene="yfcD"
                     /locus_tag="ACHUPG_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR0860137.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12053..12604)
                     /gene="yfcE"
                     /locus_tag="ACHUPG_000377"
     CDS             complement(12053..12604)
                     /gene="yfcE"
                     /locus_tag="ACHUPG_000377"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR0860138.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12660..13304)
                     /gene="yfcF"
                     /locus_tag="ACHUPG_000378"
     CDS             complement(12660..13304)
                     /gene="yfcF"
                     /locus_tag="ACHUPG_000378"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR0860139.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13440..14087
                     /gene="yfcG"
                     /locus_tag="ACHUPG_000379"
     CDS             13440..14087
                     /gene="yfcG"
                     /locus_tag="ACHUPG_000379"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR0860140.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14144..14506
                     /gene="folX"
                     /locus_tag="ACHUPG_000380"
     CDS             14144..14506
                     /gene="folX"
                     /locus_tag="ACHUPG_000380"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR0860141.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14527..15420
                     /locus_tag="ACHUPG_000381"
     CDS             14527..15420
                     /locus_tag="ACHUPG_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR0860142.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15468..16358)
                     /gene="rpnB"
                     /locus_tag="ACHUPG_000382"
     CDS             complement(15468..16358)
                     /gene="rpnB"
                     /locus_tag="ACHUPG_000382"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR0860143.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16555..17328)
                     /gene="hisP"
                     /locus_tag="ACHUPG_000383"
     CDS             complement(16555..17328)
                     /gene="hisP"
                     /locus_tag="ACHUPG_000383"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR0860144.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17336..18052)
                     /locus_tag="ACHUPG_000384"
     CDS             complement(17336..18052)
                     /locus_tag="ACHUPG_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0860145.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18049..18735)
                     /gene="hisQ"
                     /locus_tag="ACHUPG_000385"
     CDS             complement(18049..18735)
                     /gene="hisQ"
                     /locus_tag="ACHUPG_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR0860146.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18825..19607)
                     /gene="hisJ"
                     /locus_tag="ACHUPG_000386"
     CDS             complement(18825..19607)
                     /gene="hisJ"
                     /locus_tag="ACHUPG_000386"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR0860147.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19828..20610)
                     /gene="argT"
                     /locus_tag="ACHUPG_000387"
     CDS             complement(19828..20610)
                     /gene="argT"
                     /locus_tag="ACHUPG_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR0860148.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20876..21445)
                     /gene="ubiX"
                     /locus_tag="ACHUPG_000388"
     CDS             complement(20876..21445)
                     /gene="ubiX"
                     /locus_tag="ACHUPG_000388"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR0860149.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21540..23057)
                     /gene="purF"
                     /locus_tag="ACHUPG_000389"
     CDS             complement(21540..23057)
                     /gene="purF"
                     /locus_tag="ACHUPG_000389"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR0860150.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23094..23582)
                     /gene="cvpA"
                     /locus_tag="ACHUPG_000390"
     CDS             complement(23094..23582)
                     /gene="cvpA"
                     /locus_tag="ACHUPG_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR0860151.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23841..24503)
                     /gene="dedD"
                     /locus_tag="ACHUPG_000391"
     CDS             complement(23841..24503)
                     /gene="dedD"
                     /locus_tag="ACHUPG_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR0860152.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24493..25761)
                     /gene="folC"
                     /locus_tag="ACHUPG_000392"
     CDS             complement(24493..25761)
                     /gene="folC"
                     /locus_tag="ACHUPG_000392"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR0860153.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25831..26745)
                     /gene="accD"
                     /locus_tag="ACHUPG_000393"
     CDS             complement(25831..26745)
                     /gene="accD"
                     /locus_tag="ACHUPG_000393"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR0860154.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26901..27560)
                     /locus_tag="ACHUPG_000394"
     CDS             complement(26901..27560)
                     /locus_tag="ACHUPG_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0860155.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27643..28455)
                     /gene="truA"
                     /locus_tag="ACHUPG_000395"
     CDS             complement(27643..28455)
                     /gene="truA"
                     /locus_tag="ACHUPG_000395"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR0860156.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28455..29468)
                     /locus_tag="ACHUPG_000396"
     CDS             complement(28455..29468)
                     /locus_tag="ACHUPG_000396"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR0860157.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29534..30670)
                     /gene="pdxB"
                     /locus_tag="ACHUPG_000397"
     CDS             complement(29534..30670)
                     /gene="pdxB"
                     /locus_tag="ACHUPG_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR0860158.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30769..31764
                     /gene="flk"
                     /locus_tag="ACHUPG_000398"
     CDS             30769..31764
                     /gene="flk"
                     /locus_tag="ACHUPG_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR0860159.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31761..32939)
                     /locus_tag="ACHUPG_000399"
     CDS             complement(31761..32939)
                     /locus_tag="ACHUPG_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0860160.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33194..34435)
                     /gene="fabB"
                     /locus_tag="ACHUPG_000400"
     CDS             complement(33194..34435)
                     /gene="fabB"
                     /locus_tag="ACHUPG_000400"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR0860161.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            34594..36600
                     /gene="mnmC"
                     /locus_tag="ACHUPG_000401"
     CDS             34594..36600
                     /gene="mnmC"
                     /locus_tag="ACHUPG_000401"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR0860162.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36721..36999)
                     /locus_tag="ACHUPG_000402"
     CDS             complement(36721..36999)
                     /locus_tag="ACHUPG_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR0860163.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(37033..37581)
                     /gene="epmC"
                     /locus_tag="ACHUPG_000403"
     CDS             complement(37033..37581)
                     /gene="epmC"
                     /locus_tag="ACHUPG_000403"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR0860164.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37581..38390)
                     /locus_tag="ACHUPG_000404"
     CDS             complement(37581..38390)
                     /locus_tag="ACHUPG_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFR0860165.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38390..39214)
                     /gene="mepA"
                     /locus_tag="ACHUPG_000405"
     CDS             complement(38390..39214)
                     /gene="mepA"
                     /locus_tag="ACHUPG_000405"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR0860166.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39218..40303)
                     /gene="aroC"
                     /locus_tag="ACHUPG_000406"
     CDS             complement(39218..40303)
                     /gene="aroC"
                     /locus_tag="ACHUPG_000406"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR0860167.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40338..41270)
                     /gene="prmB"
                     /locus_tag="ACHUPG_000407"
     CDS             complement(40338..41270)
                     /gene="prmB"
                     /locus_tag="ACHUPG_000407"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR0860168.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            41436..41987
                     /gene="smrB"
                     /locus_tag="ACHUPG_000408"
     CDS             41436..41987
                     /gene="smrB"
                     /locus_tag="ACHUPG_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR0860169.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42091..42981)
                     /locus_tag="ACHUPG_000409"
     CDS             complement(42091..42981)
                     /locus_tag="ACHUPG_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR0860170.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(42968..43492)
                     /locus_tag="ACHUPG_000410"
     CDS             complement(42968..43492)
                     /locus_tag="ACHUPG_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0860171.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(43489..43959)
                     /locus_tag="ACHUPG_000411"
     CDS             complement(43489..43959)
                     /locus_tag="ACHUPG_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0860172.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(43956..44504)
                     /locus_tag="ACHUPG_000412"
     CDS             complement(43956..44504)
                     /locus_tag="ACHUPG_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0860173.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(44509..45231)
                     /locus_tag="ACHUPG_000413"
                     /pseudo
     CDS             complement(44509..45231)
                     /locus_tag="ACHUPG_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281619.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(45251..47891)
                     /gene="yfcU"
                     /locus_tag="ACHUPG_000414"
                     /pseudo
     CDS             complement(45251..47891)
                     /gene="yfcU"
                     /locus_tag="ACHUPG_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(47973..48536)
                     /locus_tag="ACHUPG_000415"
     CDS             complement(47973..48536)
                     /locus_tag="ACHUPG_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0860174.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        12131 a        13087 c        12013 g        11553 t
ORIGIN      
        1 gcttaacaat agtttcacac cttaccccca ttgcgacacc tcggaaatca tcacgctgtt
       61 aaactctccg taatgcggaa atatcatcat cgcgtttcat tagtgaattc ttaaatgagg
      121 cattttcaca caattatctt acagataaaa aaaccagact tgcaattaag aattagaaca
      181 attcactata taacattgca tgtaaagcat ataagcctaa ttatttgtca ttattaagtt
      241 attaacagca caatcgagcc ttcccctctg gcaaaatctt attctgcaga ccttcaaaac
      301 accgtcctgg gggagtacat tgttctaagc tgacttccac ggcagggagt ggcgataaca
      361 gcaaaaaagg tcaagattca tgtcccccat tgaaaaatcc agcaaattag agaatgtctg
      421 ttatgacatc cgtggtccgg tgctgaaaga agcaaaacgc ctggaagaag aaggtaacaa
      481 ggtactgaaa ctgaacatcg gcaacccagc cccattcggt tttgacgcgc cagatgaaat
      541 cctcgttgac gtgatacgca acctgcctac cgctcaaggg tattgcgatt ccaaagggct
      601 ttactccgcg cgtaaagcca tcatgcagca ctaccaggct cgtggcatgc gtgatgttac
      661 cgtggaagat atttacatcg gcaatggtgt atcggagctt atcgttcagg caatgcaggc
      721 attgctgaac agcggggacg aaatgttggt tcctgcacca gattacccac tctggaccgc
      781 ggcggtttcg ctttccagcg gtaaagcggt gcattatctt tgcgatgaat cctctgactg
      841 gttcccggac ctcgatgata ttcgcgctaa aattacgcct cgtacgcgtg ggattgttat
      901 tatcaaccca aataacccaa ccggcgcggt atattccaaa gagcttttaa tggagattgt
      961 ggagattgca cgtcagcata atctcattat cttcgccgat gaaatttatg acaaaattct
     1021 ctacgacgat gctgagcatc actcaattgc gccgctggca cctgacctgc tgaccattac
     1081 ctttaacgga ctgtcgaaaa cgtaccgcgt tgcaggcttc cgtcaggggt ggatggtgtt
     1141 gaacgggccg aaaaaacacg ccaaaggcta catcgaaggt ctggaaatgc tggcttcaat
     1201 gcgcctgtgt gctaacgttc ctgcgcaaca cgccattcag accgcgctgg gtggttatca
     1261 gagcatcagt gaatttatta cccctggcgg tcgtctttat gagcagcgta accgcgcgtg
     1321 ggaactgatc aacgatattc cgggcgtttc ctgcgtgaaa cctcgtggtg cgctgtatat
     1381 gttcccgaaa atcgacgcca aacgctttaa cattcatgac gatcagaaaa tggtgctgga
     1441 tttcctgttg caggaaaaag ttctgttggt gcaagggacg gcattcaact ggccgtggcc
     1501 ggatcacttc cgcattgtca cgctaccgcg tgtcgatgat atcgagctgt ctttgagcaa
     1561 gttcgcgcgt ttcctttctg gttatcatca gctgtaatct taatttcact gccggagatt
     1621 gcatccggca gtgttatccc gccacaatga cctgatgatg tcatcatacg taaggtcact
     1681 atgaaacaga gccatttttt tgcccatctc tcccgcctga aactcattaa ccgctggccg
     1741 ctaatgcgca acgtgcggac ggaaaatgtg tccgaacaca gtttgcaggt agcgatggtc
     1801 gcccatgcgc tggcagctat caaaaatcgg aaatttggcg gtaatgtcaa cgccgaacgt
     1861 atcgctttac tggcgatgta ccacgatgcc tcagaagtgc tcaccggcga tctccctact
     1921 ccggtgaaat acttcaatgc gcaaatcgct caggagtaca aggctattga aaaaatcgct
     1981 cagcagaaac tggtcgatat ggttccggaa gaactgcggg atatctttgc gccgttaatt
     2041 gacgagcatg catatagcga tgaagaaaaa tcgctggtga aacaggcaga tgcactgtgt
     2101 gcatatctga aatgtctgga agaactcgcg gccggaaata atgaattctt gctggcaaaa
     2161 acgcgactgg aagcgacgct tgaagcgcgt cgcagccagg agatggacta cttcatggaa
     2221 gtatttgttc ccagcttcca tctttcgctc gatgagatta gccaggattc accgctgtaa
     2281 gcagccggag tctgcgtcgc atcagtcaat aagcgccgga tgcgacatca ggctcttgtc
     2341 aaaacggaaa cagcatcggg atcatcacca cacaaaccgc catcacgata atggtgaacg
     2401 gtacccccaa cttcacaaag tcactgaagc tgtaatttcc cggacctaaa accagggtgt
     2461 taacaggtga agacaccggt gtcataaagg cggcggatgc tgccatcgcc acgaccatcg
     2521 caaatggata aggcgacact cccatcgttt tggcagcagc cagcgcaatc ggagccatca
     2581 acaccgccgt cgcggtatta gagataaaca gcccaataac cgccgacaag acaaacaaac
     2641 accccagcat catatgtggc ccataaccgc cgccaatatc catcagccct ttcaccgcca
     2701 gcgcgacacc tcccgttttc tgtaatgcca cagcaaacgg catcatccca acgatcaaaa
     2761 taatgctcgg ccagtgaatg gatttatagg cgctttcagc atctatacag cggaatttcc
     2821 ccatcagcag gcaggcgatg atagcggcaa caggattagg aatttcattt gtcagcatta
     2881 acgccaccat tagcaccaga cagaaaatgg catggggtgc ctggctgtgc gcgggcgatg
     2941 cttcactcac ctcttccggt aagttcagcg ctacgaagtc gcggccctgt ttggccagca
     3001 taccgatcag tttccagtta cccacaacca ggatgatatc gcccagcagc agaggctcat
     3061 ccgccagcga accttccagc gccacgccat tgcgcttgag ccccaccaca ttcagtccgt
     3121 agcgggtacg aaaaccaatt tcgcgcaccg atttaccaat cagttctgac tcaggaatta
     3181 atgaaatctc tgccatgccc acatcaaggg cctggtcaga aaaatactcg ccgcgcagta
     3241 ccatcggctc cagcaattgc tcactacaaa attgccggag atcgacatca gccgcagaca
     3301 tatcaataag caaaacgtca cgcgcgcgaa attcagaaac cccattaacg ttcacgataa
     3361 cgcgacgaaa acgccgccag cgttcaacac cgatgacgtt agcgccataa cgctcacgta
     3421 atttgagatc atccagccgt tgacctatca ttggcgatcc ggggcgaata gccagacgtc
     3481 gcgcacgccc ggtcagtcga tattcacgga taagatcgcg aaaggttcga cgcgtccagc
     3541 cttcgcgctg cggggtctgg gtatcccctt tcagcatgaa acgcatcact aacatataca
     3601 aaatacccag caccagcacg accaggccaa taggtgttac gctaaagaaa ctgaagccgt
     3661 gatagccttc acgcagcaat tcactgttga ctaccaggtt cggcggcgtc gccaccagcg
     3721 tcatcatgcc gctaatcagc ccggcaaaac ttaacggcat catcagacgt gacggcgaag
     3781 tttgcatacg catggcaacg cttaacacca cgggaataaa gatagcgaca acgctggttg
     3841 aactcataaa cgcgccaagc cccgcgacgg tcagcatcaa caaaaccaac attttgattt
     3901 cactgttgcc cgccactttg accagccatg ttcccattac ggtggcaaca ccggtacgga
     3961 ccaaaccatc gccaataata aacaaggcgg caatcaggac aacgttagga tcagaaaagc
     4021 cggaaaatac ttcggggacc gtcagcgttc cgcttaatgc aaacgcgaca ataacaaaca
     4081 aagcgaccgc atccatacgc actctgcccg tcgcaaacaa gacgatggca acggccagta
     4141 atgaaagaac ccaaatcaat tcaccgttca caacttatcc ttgttaattg agggggatga
     4201 cttgattctg ccataaaaaa gccccgacga gacggggcta aatcatgatc aggtgtttca
     4261 ctgaataata acatcgccat ttggctgttt ggtcacagta atttgctcca gactgtggag
     4321 gaccaaatcg acctcattca ggcgcggggt atctgccgga gcgttaaccg caatgacatg
     4381 gcaacccgcc gccaggccag aaagcacgcc agcgggagca tcttccacca ccacacactc
     4441 ctgcggcgca agccccagca gctgcgcacc taacagatac gcatcaggtt ctggttttcc
     4501 gcgcttcact cgctcagcgg ttacaaacac ctctggtgcg ggaagccccg ctattttatg
     4561 gcgcgctcgc gctaccggca tggagccaga agtcacaatg gcccacggaa tacctgcttt
     4621 attcaaatga ttgagtaagg cgattgcccc aggaagcgcg gtaatacctt cggtttccgt
     4681 ggcctcgatt tgctccagac gcgtaaactc ggcggcaata tcagcctcgg atttgcccgc
     4741 cataaaatgg cgcaaagagg tgatcgcctg tttaccgtga atgaaagcca gcacctcttc
     4801 cggcgctaac ccatgacgtc tggcccagtt gctccacgcc cgttctaccg caggcaggga
     4861 atccaccagc gttccatcaa gatcaaacag aaaacctttg caccgcacgc gggcctcctc
     4921 aggcgttgat aatttgatta atttcgttgg cgctcaaatg gtactgacgc gggcaggcat
     4981 gccacacatt aagcatgcgc tgatattttt cccacattgg cgtctgggcg ttaaaaccat
     5041 gagtcccagc gtcaaaatgg gtatagcgcc cttccacatt aaccataaag cggacataac
     5101 cgaggtaacg tgcttcagtg gcggcgtcaa agccgaggaa ggtgacacga cgttcatcga
     5161 tggattgctg gtcctgcaaa ttagaccaag aaacatacaa cgcatgatac atatccataa
     5221 tgtcgatgat agtgcggcag gtttcttctt tcagctcgcc aaactcgcga tccaattcac
     5281 gcatctgtaa tccgtaacca cgctcaataa ttgtttgcag gcgacggtaa cgttcagcat
     5341 ttgccggatc gagcatagtc atcatcttgt actgattaga caaaataaga cgttgcgcgt
     5401 tggtcatttc cattgttgac tcctgtatca ctctactacg gtgaaaaaaa agaaggctga
     5461 gtatgccttc ttttatatgc gtaatcaggg gtcaattaca aatcatcaag gaaagtttta
     5521 tccagttgtt tgaaggcgcg cttaagcgtg tcagctaatg cctggtaatc aggcttgcct
     5581 tcaacgggtg ccaacacctg tccagactcc tgcaatttac cgcgaacttc ataaaaccag
     5641 ttaaggattg cagggggtaa tggcgttaca gaacgcttgc ccagccacca caatccctgc
     5701 atgggtaaac ttaaggcgaa cagtgcagtg gcaactgccg gcccaagcag accgcccagg
     5761 gcaatctgcc agcagagagt aaatacggcg atcggcggca taaaacggat cgcataacgc
     5821 gtcatcttga taacgcgatt ttcgacaaag accggagcaa ggcgtttttc cagtggccac
     5881 gtctttgagt aatgctgtcc gcggcgaaac aagctaaaaa aattaacaga acgattatcc
     5941 ggcgttgaca tgcttcacct caacttcaca tataaagatt caaaaatttg tgcaaattca
     6001 caactcagcg ggacaacgtt caaaacattt tgtcttccat acccactatc aggtatcctt
     6061 tagcagcctg aaggcctaag tagtacatat tcattgagtc gtcaaattca tatacattat
     6121 gccattggct gaaaattacg caaaatggca tagactcaag atatttcttc catcatgcaa
     6181 aaaaaatttg cagtgcatga tgttaatcat aaatgtcggt gtcatcatgc gctacgctct
     6241 atggctccct gacgtttttt tagccacgta tcaattatag gtacttccat gtcgagtaag
     6301 ttagtactgg ttctgaactg cggtagttct tcactgaaat ttgccatcat cgatgcagta
     6361 aatggtgaag agtacctttc tggtttagcc gaatgtttcc acctgcccga agcacgtatc
     6421 aaatggaaaa tggacggcaa taaacaggaa gcggctttag gtgcaggcgc cgctcacagc
     6481 gaagcgctca actttatcgt taatactatt ctggcacaaa aaccagaact gtctgcgcag
     6541 ctgactgcta tcggtcaccg tatcgtacac ggcggcgaaa agtataccag ctccgtagtg
     6601 atcgatgagt ctgttattca gggtatcaaa gatgcagctt cttttgcacc gctgcacaac
     6661 ccggctcacc tgatcggtat cgaagaagct ctgaaatctt tcccacagct gaaagacaaa
     6721 aacgttgctg tatttgacac cgcgttccac cagactatgc cggaagagtc ttacctctac
     6781 gccctgccgt acaacctgta caaagagcac ggcatccgtc gttacggcgc gcacggcacc
     6841 agccacttct atgtaaccca ggaagcggca aaaatgctga acaaaccggt agaagaactg
     6901 aacatcatca cctgccacct gggcaacggt ggttccgttt ctgctatccg caacggtaaa
     6961 tgcgttgaca cctctatggg tctgaccccg ctggaaggtc tggtcatggg tacccgttct
     7021 ggtgatatcg atccggcgat catcttccac ctgcacgaca ccctgggcat gagcgttgac
     7081 gcaatcaaca aactgctgac caaagagtct ggcctgctgg gtctgaccga agtgaccagc
     7141 gactgccgct atgttgaaga caactacgcg acgaaagaag acgcgaagcg cgcaatggac
     7201 gtttactgcc accgcctggc gaaatacatc ggtgcctaca ctgcgctgat ggatggtcgt
     7261 ctggacgctg ttgcattcac tggtggtatc ggtgaaaatg ccgcgatggt tcgtgaactg
     7321 tctctgggca aactgggcgt gctgggcttt gaagttgatc atgaacgcaa cctggctgca
     7381 cgtttcggca aatctggttt catcaacaaa gaaggtaccc gtcctgcggt ggttatccca
     7441 accaacgaag aactggttat cgcgcaagac gcgagccgcc tgactgcctg atttcacacc
     7501 gccagctcag ctggcggtgc tgttttgtaa cccgccaaat cggcggtaac gaaagaggat
     7561 aaaccgtgtc ccgtattatt atgctgatcc ctaccggaac cagcgtcggt ctgaccagcg
     7621 tcagccttgg cgtgatccgt gcaatggaac gcaaaggcgt tcgtctgagc gttttcaaac
     7681 ctatcgctca gccgcgtacc ggtggcgatg cgcccgatca gactacgact atcgtgcgtg
     7741 cgaactcttc caccacgacg gccgctgaac cgctgaaaat gagctacgtt gaaggtctgc
     7801 tttccagcaa tcagaaagat gtgctgatgg aagagatcgt cgcaaactac cacgctaaca
     7861 ccaaagacgc tgaagtcgtt ctggttgaag gtctggtccc gacacgtaag caccagtttg
     7921 cccagtctct gaactacgaa atcgctaaaa cgctgaatgc ggaaatcgtc ttcgttatgt
     7981 ctcagggcac tgacaccccg gaacagctga aagagcgtat cgaactgacc cgcaacagct
     8041 tcggtggtgc caaaaacacc aacatcaccg gcgttatcgt taacaaactg aacgcaccgg
     8101 ttgatgaaca gggtcgtact cgcccggatc tgtccgagat tttcgacgac tcttccaaag
     8161 ctaaagtaaa caatgttgat ccggcgaagc tgcaagaatc tagcccgctg ccggttctcg
     8221 gcgctgtgcc gtggagcttt gacctgatcg cgactcgtgc gatcgatatg gctcgccacc
     8281 tgaatgcgac catcatcaac gaaggcgaca tcaatactcg ccgcgttaaa tccgtcactt
     8341 tctgcgcacg cagcattccg cacatgctgg agcacttccg tgccggttct ctgctggtga
     8401 cttccgcaga ccgtcctgac gtgctggtgg ccgcttgcct ggcagccatg aacggcgtag
     8461 aaatcggtgc cctgctgctg actggcggct acgaaatgga cgcgcgcatt tctaaactgt
     8521 gcgaacgtgc tttcgctacc ggcctgccgg tatttatggt gaacaccaac acctggcaga
     8581 cctctctgag cctgcagagc ttcaacctgg aagttccggt tgacgatcac gaacgtatcg
     8641 agaaagttca ggaatacgtt gctaactaca tcaacgctga ctggatcgaa tctctgactg
     8701 ccacttctga gcgcagccgt cgtctgtctc cgccagcgtt ccgttatcag ctgactgaac
     8761 tggcgcgcaa agcgggcaaa cgtatcgttc tgccggaagg tgacgaaccg cgtaccgtta
     8821 aagcagccgc tatctgtgct gaacgtggta tcgcaacttg cgtactgctg ggtaatccgg
     8881 cagagatcaa ccgtgttgca gcgtctcagg gtgtagaact aggtgcaggc attgaaatcg
     8941 ttgatccaga agtggttcgc gaaagctatg ttggtcgtct ggtcgagctg cgtaagaaca
     9001 aaggcatgac cgaaaccgtt gcccgcgaac agctggaaga caacgtggtg ctcggtacgc
     9061 tgatgctgga acaggatgaa gttgatggtc tggtttccgg tgctgttcac actaccgcaa
     9121 acaccatccg tccgccgctg cagctgatca aaactgcacc gggcagctcc ctggtatctt
     9181 ccgtgttctt catgctgctg ccggaacagg tttacgttta cggtgactgt gcgatcaacc
     9241 cggatccgac cgctgaacag ctggcagaaa tcgcgattca gtccgctgat tccgctgcgg
     9301 ccttcggtat cgaaccgcgc gttgctatgc tctcctactc caccggtact tctggtgcag
     9361 gtagcgacgt agaaaaagtt cgcgaagcaa ctcgtctggc gcaggaaaaa cgtcctgacc
     9421 tgatgatcga cggtccgctg cagtacgacg ctgcggtaat ggctgacgtt gcgaaatcca
     9481 aagcgccgaa ctctccggtt gcaggtcgcg ctaccgtgtt catcttcccg gatctgaaca
     9541 ccggtaacac cacctacaaa gcggtacagc gttctgccga cctgatctcc atcgggccga
     9601 tgctgcaggg tatgcgcaaa ccggttaacg acctgtcccg tggcgcactg gttgacgata
     9661 tcgtctacac catcgcgctg actgcgattc agtctgcaca gcagcagtaa tctcgtcatc
     9721 atccacagct ttgcgctgcg gatatctgaa ccggaaataa tcactatttc cggtttttta
     9781 ttctcttaat ttgcattaat cctttctgat tatcttgctt aactgcgctg catcaatgaa
     9841 ttgcgccatc tcactttgca tacttaccac tttgttttgt gcaagggaat atttgcgcta
     9901 tgtccgcaat cactgaatcc aaaccaacaa gaagatgggc aatgcccgat acgttggtga
     9961 ttatcttttt tgttgctatt ttaaccagtc ttgccacctg ggtagttccg gtggggatgt
    10021 ttgacagtca ggaagtgcag tatcaggttg atggtcaaac aaaaacacgc aaagtcgtag
    10081 atccacactc atttcgcatt ctgactaacg aagcaggcga acctgagtat caccgcgtac
    10141 agctgttcac gacgggcgat gaacgcccgg gcctgatgaa cttcccgttt gaagggttaa
    10201 cctcaggatc gaaatacggg acagccgttg gcatcatcat gtttatgctg gtgattggcg
    10261 gcgcgtttgg cattgtgatg cgtacaggaa ccattgataa cggtatcctg gcgcttattc
    10321 gccatacccg cgggaatgaa attctcttta ttcctgcgct gtttattctg ttttcacttg
    10381 gcggcgcggt atttggtatg ggagaagagg ccgtcgcctt tgccattatc atcgcaccgc
    10441 taatggtccg gctgggctat gacagtatta ccaccgtcct ggtgacttat attgccacgc
    10501 aaatcggttt tgccagttcg tggatgaacc cgttttgtgt ggttgttgct caggggattg
    10561 ccggcgttcc ggtgctttct ggctccgggt tgcgcatcgt ggtgtgggtt atcgccactc
    10621 tgattggcct gatctttacc atggtgtacg cctcacgagt gaaaaagaat cctcttctgt
    10681 cacgcgtgca tgagtccgac cgcttctttc gtgaaaagca ggcggatgtt gaacaacgtc
    10741 cgtttacctt tggtgactgg ctggtattga ttgtcctgac agccgtaatg gtctgggtga
    10801 tttggggcgt gatcgttaat gcctggttta ttccagaaat tgccagccag ttcttcacca
    10861 tgggtctggt gattggcatc atcggcgttg ttttccgcct taacggcatg acggttaata
    10921 ccatggcttc atcctttacc gaaggggcgc gaatgatgat cgcccctgcc ctgctggtgg
    10981 gtttcgccaa agggattttg ctgctggttg gtaatggtga agcaggtaat gccagcgtgt
    11041 taaatactat cctcaacagc attgccaatg ccattagcgg tctggacaac gcggtcgcgg
    11101 cctggtttat gttgctcttc caggctgtat ttaatttctt cgtgacgtcc ggttctggtc
    11161 aggcggcatt aactatgccg ttactggcac cgcttggcga tctggtcggt gttaaccgtc
    11221 aggttaccgt gctggcgttc cagtttggtg atggcttcag ccacatcatt tacccaacct
    11281 cagcttcgtt aatggcaacg ctcggtgtgt gccgggtgga cttccgtaac tggctgaagg
    11341 tgggcgcgac cctgcttgga ctgctgttta ttatgtccag cgtcgtagtg atcggcgctc
    11401 agttgatggg ctaccactaa aaatgttaag agccgcaatt gcggctcttt tttcattctg
    11461 ccgtttcagt ctctacggct tcatttttgg cattgcgctt catccacaac gccagcgctt
    11521 tcagcgagtc tggagtgaac tcatcgcagc gtgcggtgat ttcttccggc gtcagccagc
    11581 aaactccact gacttcatct tcctgtaggg cgaagggacc gtgagagacg caactgaaca
    11641 acgcgcccca gacacggcaa tttttatctt cgaaatagaa ctgcccgtgc tcggcaaagg
    11701 ggacaccggc aatgcccaac tcttcttccg cttcgcgacg cgcggattcc agcagttgct
    11761 catcggcctg aactacaccg cctgcggtcg catctaacat gccgggtaaa aagtcttttg
    11821 tctcggtacg acgctggacc agaattttgc ccatgccatc atgcacgacg atgtaagttg
    11881 cacgatgacg cagacactgt gcccgcattt gttcccggct ggcttgtgca atgacttcgt
    11941 tctcttcatt gacaatatcc acccattcag tacttgccaa acgacgctgt tccattatca
    12001 ggaaaccttc tttttctggc gctcttacgg cgcattttga gttgtgggta aattacggat
    12061 taatcgcgac ctgcgcaatg atactttgat cattgagtgc gattacacta agtacgtcat
    12121 tatccagcat gccataaccc gccggattac cgcctttcgg aatactcacc gagccggggt
    12181 tgaagtggaa aatctccccc cgctgttctg ccactggcag atgggtatga ccgtacacca
    12241 gcacatcgtt ctggtttaaa gcaggtagat tttccgggcc aaaaagatga ccatgcgtca
    12301 aaaacagacg ttgtttttcc agtaatacct gttgccacgg cgcggttatc gggaaatgca
    12361 gcatcatttg atccacttcg ctgtcgcagt tgccacgcac agcgataacc ttatgtgcca
    12421 cttcattaag ccgttcagcg actttggctg gcgcgtaacc ctccggtaaa gcattacgcg
    12481 ggccgtgatt caacacgtcg ccaaggatca ccagccactg ggcaccgctt tggacaaaca
    12541 actccacaac acgttccgtc gccggtaacg acccatgaat gtccgatgca aacatcagtt
    12601 tcatcactca ctcctcgtcg aaaaaacgta agctatgata ctggattctg tcgttgctat
    12661 cagccagatt gcttcgccga aagtgcaata aaacgctgga cagacgctcg ctgccactgg
    12721 aatgtcgcat aatccaccag gcgttctggc acctcatcgc catgtagtac caggcggtta
    12781 atcattagcg ccagatcagt atcagcaatg caccattcac caaataaatt cggctgaccc
    12841 agtgctaaca aatgttctgc catcgcgaac agtttctctg cactggcttt tccctcggcc
    12901 gttagtggcg cttttttcgc ccccgcaaag acaacatccg tcggacgctc ttcgcggatg
    12961 ggcatcagat cgctgcgcag ccaggcctga atctgtcgtg cacgcgcacg attttctaaa
    13021 tcaagcggat aaatacgttc ccaggtcggt ggcgcaaatc gatcttccag atactccgca
    13081 atggcagaag attcactcaa ctcaaaatca tcgatttgta ataacggcac acggcgtgtc
    13141 tgaccgtaac cttgccacgt cggctgcaaa tgttcaccgc tgtcgaggtc gatggtcttg
    13201 atatgaaacg acaggccttt ttcctgcaac gccacccagg cggataacac ataaggggag
    13261 aaaaagtggg catctgacca aagcgtgata gcgggtttac tcattatgtc ctcggcaata
    13321 aagggggctg gctatcaaac tatagagcct ttttgtcact gtcacgtgac gaatactcac
    13381 gtaagtcgcc gcgctttcta tacttgtggt gttcagatga ttaaacaacc ggagctgcaa
    13441 tgatcgatct ctatttcgcc ccgacaccca atggtcacaa aattacgctg tttctcgaag
    13501 aagcagagct tgattatcgc ttgattaagg tagacttggg gaaaggcggt cagtttcgtc
    13561 cggaattttt gcgcatttcg cccaacaaca aaattccggc aattgttgat cattctcctg
    13621 ccgatggcgg cgaaccgtta agcctctttg aggctgctgc cattttgttg tatctggctg
    13681 agaaaacagg actctttttg agtcatgaaa cgcgtgagcg cgccgccaca ttacagtggt
    13741 tattctggca ggtaggcgga ctggggccga tgcttgggca aaatcatcat tttaatcacg
    13801 cagcccccca aaccattcct tacgctattg aacgttatca ggttgaaact cagcgtcttt
    13861 accatgtact aaacaagcgg ctggaaaact cgccctggct gggaggcgag aactacagca
    13921 ttgcggatat tgcctgctgg ccgtgggtta atgcctggac tcgcctgcga attgacctcg
    13981 caatgtatcc ggcagtcaag aactggcatg agcggatccg ttcgcgccct gccaccgggc
    14041 aggcactgct aaaagcacaa ctcggtgatg agcgttcgga tagttaacag aaacaggttc
    14101 tcgtgtatta tttcatccta agtaaaacaa cggagaacct gcaatggcac aacctgccgc
    14161 tattattcgt ataaagaacc ttcgtttgcg tacgtttatc ggaattaagg aagaagaaat
    14221 taacaaccgt caggatattg ttatcaatgt gacgatccac taccccgccg ataaagcgcg
    14281 cactagcgaa gatatcaacg atgcgctgaa ttatcgcacc gtaacgaaaa acattattca
    14341 gcatgtagag aataaccgtt tctctttgct ggaaaaatta actcaggatg tgctcgatat
    14401 cgcacgtgaa catcactggg tgacgtatgc tgaagtggag atcgataaac tgcacgcgct
    14461 gcgctacgcc gattcggtat ccatgacctt aagctggcag cgttaatcgc catatcggga
    14521 ggctgcatga acatagtgat caccggaggg acgggattaa ttggtcgcca tttgattccg
    14581 cgtttgctgg agctgggcca tcaaattacg gtagtgacgc gtaacccgca gaaagccagt
    14641 tccgttctcg gccctcgggt gacactatgg caagggcttg ccgatcaaag caacctcaac
    14701 ggcgttgatg cggtaatcaa cctggccgga gaaccgattg ctgataaacg ctggactcac
    14761 aagcaaaaag agcgtctctg ccaaagccgc tggaatatca cgcaaaaact ggtcgatttg
    14821 attaatgcta gcgacacgcc gccgtcggta ctcatttccg gctcggcaac gggctattat
    14881 ggcgacttag gtgaagtggt ggttaccgaa gaggaaccgc cgcataacga atttactcat
    14941 aaactctgcg cccgctggga agagattgcc tgccgggcgc aaagtgacaa aacgcgagtg
    15001 tgcctgctgc gaaccggcgt ggtactggca ccggacggcg gtattctcgg taaaatgctg
    15061 ccaccgtttc gtcttggcct gggcgggccg attggttccg gtcggcagta tctggcctgg
    15121 attcatattg atgatatggt caacggcatt ctctggctgc tggataacga gctgcgcggg
    15181 ccatttaata tggtttcgcc ctacccggta cgcaatgaac aatttgccca tgcgctcggt
    15241 catgcgctgc atcgcccggc tattttgcgc gtccccacaa ccgccattcg gctgttaatg
    15301 ggcgaatctt cagtactggt attaggtgga caacgcgcgc tgcctaaacg gctggaagaa
    15361 gcgggttttg cgtttcgctg gtacgattta gaagaggcgc tggcggatgt cgttcgctga
    15421 tgtggtttac agcaaacatc cgccagttaa ctccctgtgt tacaggatta gtggctttgc
    15481 gcgataagat cgtctggtga aagtcgggtc accatcataa ctaactctct gtctaaacct
    15541 ctatccagca tctcctgagc aatacgcagg gcttcttcgt gtttgccctg cattgcgcct
    15601 tcttcacgta atctgtcagc aatggtcatc agtttctcct tttcttgtgg tgcgcgttct
    15661 gttatctcac caataaatgc acggaaacgc tgggcatccc ctgtttgtaa tacgtaatta
    15721 aacagggctt ttagctgtct gtcattagtg ttccctgtaa ctaacagcga aacaatttgg
    15781 tcgactaatc ccaacagatc gcgctgacga atatgtttct gaattaactc caacagcgcc
    15841 attttgcggt gttgcataat ctcgtcatcc ggcaccacgg taatatccac caacggaaac
    15901 gctgatgaat atattttgcg ggctatagca ggctcggcaa attcatcaag ccagcagagt
    15961 gaataaggat aaggacttct gcaaccatga taaaacagca tcgggatcac caatggaagc
    16021 tctttatagc ccgcatcaag atggttttgc attgccgcaa tggaataacg catcatgcga
    16081 aaagccatta attcttccgg cttactttgg tgctctatca ctacataaat ataacccact
    16141 ccctcctgcg ttttcacaga ccacaagagg tcggaataat attgccgcag gtcttcatca
    16201 ataaaactgt ttggttctag tttaagcgtc gttaaatcac acagtttgcg cagcggcgcg
    16261 ggaagatgaa tattaataaa attccgcgcg gtgtctggat ggcgtaaaaa agatttaaat
    16321 accgcatcat gcggcgtgga agttgtcgat attgtcatgg cgttccgtca cccctcaaat
    16381 ttaagatgac gcgacaataa ccacgattaa cacccaatgc agcaggcagt ttttatcttt
    16441 ggacagcgaa ttcagaagta tttttagcgg actgcaactt ttacatttca gcggaagctg
    16501 actcgcaaat tccccttgaa aatactccgg cagcgttagc caccggagag gaaattattt
    16561 cagcgatccc ttaaggaact gttgcagacg agggctttgc gggttgccaa ataactgctc
    16621 cggcgcgccc tcttcttcta ttttcccctg atggaggaaa atgacgtgag tagaaacatg
    16681 gcgagcaaag cccatttcgt gagtcactac caccatggtt ttcccctctt ctgccagttg
    16741 ctgcataata cgtaacactt cgcctaccag ttcaggatcg agcgccgagg taggttcatc
    16801 aaacagtaaa acttctggtt ccatcgccag cgcccgcgcg atagaaacac gctgttgctg
    16861 accgccggaa agatgcaccg gatatttccc ctgcgcacgt tcgtctatcc cgactttcgc
    16921 cagatacttc accgcccgct cgcgcgcttc ctgcttgctc aggcccaaca cctgaatcgg
    16981 cgcttccatg acgttttcca acaccgtcat atggctccag agattgaagt gctggaatac
    17041 catcgtcagg cgtgtgcgca gtaagcgcag ttgattttta tcggcgactt tgagttgacc
    17101 gtctttgtcg cgcaccagat tgatcgtctg gccattgacc acgatcgatc cttcactcgg
    17161 tttttcgagg aagttaatgc agcgcagaaa ggtacttttc cccgatcccg acgatccgat
    17221 gatgcttatt acatctccgg cattcgcttg cagtgagacc cctttcagca cttcgtgttc
    17281 gccatagcgt ttgtgcaaat cgataacgtt taatttattc tcggacatcg tattctcagt
    17341 gcgttgaaga aggtttcaca tgctgcaacc agcgtttttc cgctctgcga aagaggctga
    17401 tcaggacata agagatgatt aaatagagca ccgcggcaat gccgaaggcg gtaaaaggtt
    17461 gatacgtggc ggcgttaata tcgcgggcta ttttcagcag atccggcaca gtggccgtaa
    17521 acgccagcgc agtagagtgc agcatcagga tcacttcgtt gctgtatgcc ggtaacgcaa
    17581 tacgcagcgc agaaggcaaa ataatgcagc gatacatttt aaaggtcgag aagccatagg
    17641 ctctggcggc ttcaatttcc ccatgcggaa ccgaacggat tgccccagca aaaatctcgg
    17701 tagtgtaagc gcaggtgtta agcgtcagcg ccagcacggt acagttcagg ccactgcgga
    17761 agaaagcgtt aaggaattcg gttcccttaa caatctcaag cgtgtacatg ccggaataga
    17821 acaccagcaa ctgaacatac agcggcgtac cgcgaaaaat ataggtaaat aaccagattg
    17881 gaaactggat gtatttatta ctggagacac gaccaatcgc cagaaacagc gccaggactc
    17941 cgcctatcac taccgacaaa ataagcagcc acagagtgat cgccacacca gtaaagcgat
    18001 aaccgtcggt ccacagcagc ggtttccagt attcatgtaa gatttcgatc acaggtcagc
    18061 cctcttcaca cccacggagt agcggcgctc aaggaacagc agcacaccat tggaaacggt
    18121 ggtgaaaacc aggtaaatca cgccacagac gatggcgaaa tagaacggtt cccaggtact
    18181 tttgcctgcc agctgcgtgg ctttgaccac atcttccagg ccgagtaacg aaaccagtgc
    18241 ggtagatttg aggatcacct gccagttgtt gccaatgcct ggcagggcgt aacgcatcat
    18301 cgccggaaac atgatccgcc gaaacacttg cccgcgagta aaaccgaacg ccgtcgccgc
    18361 ctctatatgc cctttcggca ctgccataaa cgcgccacgg aacgtttcgg taaaataagc
    18421 accgtagata aaaccgagag tgataatacc agcgaccatc ggatcgatat caatctgccc
    18481 gacgcccatc gcctccgtca ccatgtttag cgcaatctgc aaaccataga aaatcagcag
    18541 catcaacacc aagtccggca cgcctcgaat cagcgtggtg tacccttcga aaatcagccc
    18601 cgaaagccga ttttgcgaga gcttaccgcc agcgccaatt aaaccgatga ttacagcgag
    18661 cactacagag ctgatagcca gctccagcgt gacgagcgca ccctgtaaaa taacacctga
    18721 aaacccatac aacatgctgc ctgtcctgtc gtgtgtggtg aattaccgcc tcttgtctcc
    18781 ctccggttac ccgaagggag cgggctttct ttcaccgatg gcgattaacc accataaaca
    18841 tcaaaatcga agtacttttt cgctaatttc tcgtaagtac cgtcagcgcg catttcggca
    18901 aaggctttgt tcagtgcctc gcgcagttcg ttatcttctt tacgcaggcc catgccggtc
    18961 cctacgccaa acagtttttc atctttaaca gacggaccac cgaatttgta atctttaccg
    19021 acgggttgtt tgaggaaacc ttcgctggca gcgacctcat cctggaacgc ggcatcaata
    19081 cgtccggcag tcaggtcaga ataaatgttg tcctgcccct gatacgagac gatttcaatg
    19141 ccttttggtg cccagtgttc attaccgaac gtctcctggg tggtgccctg caatacgcct
    19201 acccgtttgc ctttcagcga ctcgactgtc ggctgaatgt cagaattttt cgccaccacc
    19261 agacgagaat cggcagcgta cagtttgtcg gtgaaggcaa tttcttgctg acgtttttcc
    19321 gtaatggaaa gcgatgacat gatggcgtca atcttcttcg cttttaagga cgggattaac
    19381 gcatccagcg ggttttcgac aaacgtacat tgcgtattga tgcgcttgca caactccttc
    19441 gccagctcga tatcgaaacc aaccagttcg ccttgtgaat tttttgattc aaatggcgca
    19501 taggtcgggt cggtaccgat gcggatgttt tgcggaattg cagcaagtgc cgcagttgcg
    19561 ctggagaagg ccagaaccag agagagcgat agcaccagtt ttttcataat gtcctcaact
    19621 gacagtcttt ttatagggaa tatttacagg tttgttacac ttatcgtgcc ataaaattga
    19681 ccatcaaggc aaaatatccc gccccgttag acgtattatt tttcattaaa aatgcttaag
    19741 tatgcatttc aatgcaccac gatagtgcaa caaacctggc gtgcaccatg atgatcattc
    19801 catcaggtac agcttcccag cgacgtatca gtcaccgtag acattaaagt cgaaatactt
    19861 tttcgccatc ttgtcgtagg tgccgtcctg acgcagctcg ccaagcgcct tattgaaggc
    19921 agccgtcagt tcagcataat ctttacgtag ccctacaccg gtgccatcac cgaagtattt
    19981 tttgtctttt actgatgagc cagcaaaagc gaaatcttta ccagcaggtt gcttgaggaa
    20041 tccttcgctg gcagcaactt catcttgtaa cgcagcatcc agacgtcctg cagccagatc
    20101 ggaatagacc aaatcctggt tggcataggc caccacatcc acgcctttac tacgccaggt
    20161 ctcgttagcg taagcttcct gggttgatcc ctgcagcaca ccaacatgtt tacctttcag
    20221 tgaatccagc gttggctgaa tcggtgaacc tttggcagca atcaaacgag aatctgcggc
    20281 gtacagcttg tcggagaagg caatctcctg ctgacgttta tcggtaatgg aaagcgacga
    20341 aataatagcg tcgatttttt tcgctttcag tgaggggatc agcgcgtcaa agtcactggc
    20401 aacccaggta catttcacct gcatccgttt gcacatctcg ttaccgagat cgatatcaaa
    20461 gccaacaaaa tcacctttag catctttcga cgagaacagc gcataggtgg tatcggttcc
    20521 gatacgtacc gtctccggta gcgccgcata gctggaagcc gctgcggaga gaccgactaa
    20581 taaagacaga gcgagaatcg acttcttcat acataaccct caagtgaaaa tggctttttt
    20641 atgttgtgta ttgtgttgtg tgtttgcagg ctctttcatg caggtcttat gccatcttga
    20701 cgacaacagt aacattcaac gttaaatatg ttaataagac attgcattat tgtcctgaag
    20761 ttgaagatag caggtatagc ggttgaatcg cagcgtttcg attgaatggc agaaacaaaa
    20821 tgtcgaggat ttgatcgcgg ttgcaaaatt gccctgaaac agggcaacag cggagttatg
    20881 cgccctgcca gcgggcaaag agatcttcag gaagggttat tgcaaactgg tcaagaacac
    20941 gattaaccgt ctgatttatc acatcatcaa gggattgcgg gcgatgataa aacgccggaa
    21001 cgggaggcat aatcaccgca ccgatttctg ccgcctgagt cattaaacgc agatggccta
    21061 agtgcaatgg tgtttcacgc acgcagagca ccaacgggcg acgctctttc agcaccacat
    21121 ctgccgcacg ggtcagtaag ccatcagtat agctatggac aatgccggac agggttttga
    21181 ttgaacaggg taaaatcacc atccccagcg tctggaaaga accggaagag atgctggcgg
    21241 aaatatcgcg cgcatcgagc gtgacatcag ctaatgcctg cacttcgcgc agggaaaaat
    21301 ccgtttcgag ggataaggtc tggcgcgccg cctggctcat caccagatgc gtttcgatat
    21361 ctgtgacatc gcgcagaacc tgtaataagc gcacgccata aatcgcgccg ctggcaccgc
    21421 tgatgcctac aatgagtcgt ttcataaata gttgccctgt cagacttgca ggcagacttt
    21481 gcagtatttc ggcgggagtt gcaagtcagg gtgccagacc ggcaccctca gcgaaggcat
    21541 catccttcgt tatgcatttc gagattttcc acttcgttct gacgttgcac tgctttggcg
    21601 tcatcattac gtaacgtatc gaggaaatcg aggtagccct gatcaacatc tttggtgacg
    21661 tagacgccgt tgaacaccga gcattcaaac tgctggatat ccggattttc agcgcgaacg
    21721 gcgtcgatca gatcgttcag atcctggaaa atcaacccgt cagcaccgat gatctggcga
    21781 atttcatcaa cttcgcgacc gtgagcgatc agttccgtgg cgctcggcat atcaatacca
    21841 taaacgttcg ggaagcgaat ttccggtgcc gcagaagcga ggtacacttt cttcgctccg
    21901 gcttcgcgtg ccatctcgat aatctgctca gaagtggtgc cacggacgat ggagtcgtcg
    21961 accagcagga cgtttttatc gcggaactcg gcgcggttgg cattcagttt acggcgcacg
    22021 gacttacgac gcagctgctg gcccggcatg ataaaggtgc ggccaacata gcggttttta
    22081 acgaagccct ggcggtacgg tttgcccaga atacgagcaa tttccagcgc gatatcacac
    22141 gaggtttctg ggatcgggat caccacgtcg atatccagat cttcccattc gcgggcaatt
    22201 ttctcgccca gtttcgtgcc catattcaca cgcgcgctgt aaacggaaat tttgtcgata
    22261 aacgagtccg ggcgggcaaa gtatacatac tcaaacaggc acggattgct gaccggattg
    22321 tcagcacatt gacgggtaaa caactgccct tcttcagtga tgtaaatcgc ttcgcccggc
    22381 gcgacgtcac gcaggaaatc aaagcccagc gtatcgagcg ctacgctttc ggaagcgacc
    22441 atatattctg tacggttctc gtcaatatca cgttttccca gtaccagcgg acgaatcccg
    22501 tttggatcgc ggaaagcaac cataccgtgg ccgataatca tcgccacaca ggcatacgcg
    22561 ccgcggatta agcggtttgt ggcagcaatg gcagcgaaaa tattgtcggc ttccagcggg
    22621 tagtggcgga agttgtccag ctcgctggcg aagatattaa gcagaatttc cgagtcggaa
    22681 gtggtgttga tgtggcggcg tttttcttca aacagttttt tacgcaactc gtgagcgttg
    22741 gtcagattgc cgttgtgggc aagcgtaatg ccatacgggg agttaacgta aaacggctgc
    22801 gcttcagagg cgctggagct gccagccgta gggtaacgca catgaccaat gcccatattg
    22861 ccctgcaaac gctgcatatg gcgagcttca aatacatcgc tcaccagccc gttcgcttta
    22921 cgcaaacgga agcagttatt ggcatctatg gtgatgatgc cggcggcatc ctgaccgcga
    22981 tgctgaagca ccgttaaggc atcataaatc gactggttaa ccggcataac accggcgata
    23041 ccgacaatac cgcacatacg tctttttcct cgttaagcca catctcagag cacttacgct
    23101 ctgggcaaga aacttgacga gctttgcaga taatcaaaaa accatctgat gataaaactg
    23161 aattgcggga tcagctgtga tttgctccag tcttcacttt tcgacacccc ggtaaaggag
    23221 tcgagaaaga agagaatggc ggcaacaatc aacacaccgc gcaacgcacc gaaacagacg
    23281 cccagcaccc gatcggtgcc tgacaacccc gttttctcca ccaactggcc tatcacgaag
    23341 ttcacgatag caccaacgat cagggtagcg ataaacagta ccgcgatggc aatcccattt
    23401 cgaaccagtt cgtcttcaaa gcccgtaaac cagactgaca ggtaagtgta gtaatgactg
    23461 gcaacaaaga aagcacaacc ccatgtcacc agcgataacg cttcacgaac aaagccgtgg
    23521 atcaggctaa ccagagagga aaaagcaatc accgcgatta tggcgtaatc aatccagacc
    23581 atatgtgtcc cacgatttta cgccctgtca tcctgttcgg ggcgcattct aacagaaaaa
    23641 gaaaacgttt gcgtagggat ttccttcccg cgcatcaata aaaatggcgc tgaaaaaata
    23701 ttcaacgcca tcgacttttt atgcctttgc ggcatcgtgc aatgcgtgtc ggatgcggcg
    23761 taaacgcctt atccgaccta cggttctaac actgcccaag cctgataaga cgcgccagcg
    23821 tcgcatcagg caagactgta ttaattcggc gtatagccca ttaccacgcc acttaagcca
    23881 gaaagttgct tcaactcacc cagcgaacct ttcagcttat ctttcgaggc atccggccca
    23941 accagaatac gggtaatttt accctgcact ggcgtggatg gcgacgtata aacccgataa
    24001 ccggcaccgc gcagcttacc gacaatctca ttcactttat cggcattttt cagcgcaccc
    24061 agttgcacaa cataggcttt acccgttggc gctgcttttt cttccacgac cggcttcggt
    24121 tctggcttcg gcgcaggtgg tacttcaacc tttggtttag gcggctccac cggtttcggc
    24181 ttcggtgggg cgaccggtgc aggttccggt tcaaactcgg tgttattggc tgcaatagtg
    24241 gccggatcga gcgacggtgc tgcggcatca cctgcccgca cctcttccgc tgcgccttcc
    24301 ggcggctgcg tcggcaacgc ctgggtggcg gctggcatca tatcaggctc atcacgatcg
    24361 cccgctttcg gcaccagcgg gatagccgcg aactcatcct gataatgttt tttctgcccg
    24421 tccagcagcc ctggaagtac aatcaccccc agcgccacca gcacgatcgt gcccactaac
    24481 cgattctgaa acttacttgc caccgcttct cctcgcgtca atcacttcca tgacatgtgc
    24541 gaccgtgtgg aaagaaccac acaccagcac ggtgtcttcc gctttagcgt ccgccattgc
    24601 ggcatcccat gcctgcgcaa cgctatcaaa tgatttgccg ttacccaaat gctcaagcag
    24661 ttgttctgcc gtggcaccgc gcggcccttc cagtggcgca caataccagt catcaaccac
    24721 gcttttcaac caggccagag ttccggcaat atctttatca tgtagcatac cgataaccgc
    24781 cagcacgcgc ccgtttttcg gtagcgcttt catacgcccg gtgagatatt ccgccgcatg
    24841 tggattatgc gcgacatcaa aaataacgcg tggcgactcg ctcacaatct ggaaacgtcc
    24901 cggcaaaatt gcgctggcaa tcccgtcgcg aatggcattt tcactgactt ccagcccgct
    24961 ggcacgcagt gccgccagcg ccgttgcggc attcggttgc gggacaagcg gcaacggcag
    25021 attttccagc gtgccgtgag catcgctaaa cgcccagtca tgatcggtga cggaatagtt
    25081 ccactcaacg ccccgacgtt gtaacagtgc acctttttcc tgcgccacat cagcaatggt
    25141 agaaggcatt tccggctcac cgacaattgc cggtttttcg ctgcggaaga tgcctgcttt
    25201 ctcgagacca atactttcgc gatctggacc cagccagtcg gtatgatcca gcgcaatact
    25261 ggttactacc gcgacatcgg catcgacaat attggttgcg tccagacgac cgcccagccc
    25321 tacttccaga atcaccacgt caagttgtgc ctgcttgaac agccacaacg ccgacagcgt
    25381 accgtactcg aaataggtca gggaaatatc accgcgtgcc gattcaatct ccgcaaaaga
    25441 ggcggtgtgg gccgattccg gcaattcctg gccctgcaca cgtacgcgct cggtataacg
    25501 caccagatga ggcgaactgt agacgcccac tttgtaccct gccgccatca gaatcgactc
    25561 cagcgtacgg caggtggtgc ctttgccatt cgtacccgca acggtaaaca caaatggcgc
    25621 tggtttcagg acgccaagac gcgccgcgac ctggctcacg cgctcaaggc cgagatcgat
    25681 agttttactg tgcaggtttt ccagataaga aagccacgaa gccagaggcg acgcggcttg
    25741 aggagtgcgt ttgataatca tggtatccgc tgattcgtta cggtgagaat agcaaaaggg
    25801 cagagccagt ggccctgccc ttatcagtta tcaggcctca ggttcctgat ccggtaccgg
    25861 gggtaccact acgccttcac gcggcgcttc aggattcggc gctggcagat tcatcaactt
    25921 cgccagaatg ctcgccagtt tcaggcgcat ttccggacga cggacgatca tgtcgatcgc
    25981 gcctttctcg atcaggaatt cactgcgctg gaatctaggc ggcagttttt cgcgaacggt
    26041 ctgttcgata acacgcggac cggcaaagcc gattaacgct ttaggttcag cgatgttgag
    26101 atcgcccagc atggcgaaac ttgcagaaac accgcccatc gtcgggtcgg tcagcacgga
    26161 gatgtacggc aagccgcgct cctgcatttt tgccagtgcc gcagaggttt tcgccatctg
    26221 catcagcgac atcagtgctt cctgcatacg tgcgccacca gaggcggaga agcagatcag
    26281 cgggcagtta tcttcctgcg cctgctcaac ggcacgcacg aaacgtgcac ccacaacaga
    26341 ccccattgaa ccgcccataa aggcgaactc gaatgccgca gcgacaaccg gcattccata
    26401 cagagtgcct ttcatcacca ccagcgcatc tttttcgccg gtttctttct gcgcagatgc
    26461 cagacggtct ttatacttct tggagtcacg aaacttcagc tcatctttcg gctcaagctc
    26521 gctacccagc tccacaaggc ttccttcatc taacaggcta tgcaggcgat tacgcgctgt
    26581 catacgcatg tgatggtcac acttcggaca gacctcaaga ttacgttcca gctcagcgcg
    26641 gtataaaacc tgaccgcagc tatcacactt agtccacacc ccttcaggaa tgctcgcctt
    26701 gcgggtggga gtaatgttgc ttttaattcg ttcaatccag ctcattaggg acctttctgt
    26761 ctgaacctgg ttcgatgcca gttttatctt tggggacgca taatgccatt tttgccccca
    26821 acagaccatg aatgttgcac attaaaacat aacagcccga aactttggat aaaaaagtgg
    26881 tcgaaccgcg gagttacttt ttattttgcg gcgcgtgccg cagcgcgttt gtgacggatt
    26941 atttcgatga cgcccggcaa aatagaaacc acaataatcc cgacgatcag cagcttaagg
    27001 ttatcctgaa ccatcgggat tgtaccgaag aaatagcctg cgtaggtaaa aagcagtacc
    27061 cacaacagtg cgccgatcac gttataagca gcgaaatgac ggtacgacat gtggcccatt
    27121 cccgcaacaa acggggcgaa cgttctgacg atcggcacaa aacgggcgag aataatcgtt
    27181 ttgccgccat gtttctcata aaactgatgg gttttgtcga gataactgcg acggaaaatt
    27241 ttcgaattgg gattactgaa taacttttca ccgaacaacc gcccgatagt gtaattgacc
    27301 gcgtcaccca caatcgcggc gatcagcatc agcactacca tcatatggac gttgagatcg
    27361 ttggtttcca gtgatgccaa cgctccggca acaaacagca atgaatcacc cggtaaaaac
    27421 ggcattacca ccagaccggt ttcacagaac aaaattaaga acaaaatggc ataaacccag
    27481 acgccgtact ccgcgactag ctctgccaga tgcacgtcaa tgtgcaggat aaaatcgatg
    27541 aggaaataaa tcaggtccat atttgcctat gccttgtact cgtcatcttt caggctgtaa
    27601 ctacgttggc tacagcctga aatgttccga gcattattct cgttagtccg ccagaaatag
    27661 cgggcccatt ggcggttttg gaagatcata ccggtcaggg taatccaccg cgaccagata
    27721 cagcccttcc gcttttgccg ttgctgccgc cagcgttctg tcctttgccg ccagcaactc
    27781 tgctatccag ctctccggct ggttgtgggc accgacttcc atcaggctgc cgacaatatt
    27841 cctgaccata tgatgtacaa aggcattcgc tttgatatca accaccacat aaggaccgtg
    27901 acgcgtgaca ttaatatgca taacgttgcg ccacggggtt cgggactggc actgcaccgc
    27961 acggaacgag gtgaaatcat tctcgcccag caagcattgc gcagcccgat gcatccgttc
    28021 agcgtccagc ggttcgtaaa aatgggttac ccctttactc agtaccgccg ggcgcagccg
    28081 atgattgtag atgatgtagc gataacggcg agccgtggcg ctaaatcggg catgaaaatc
    28141 atcaggtaca gctttaaccc aacgcacggc gatgtcacca ggtaaattcg catttacgcc
    28201 caaggtccac gccgcgtctt tgcgctgcgc ggttgtttcg aaatgcacca cttgcccggt
    28261 accgtgtacc cctgcgtcag tacgcccggc gcagaagacg gtgatgggtt cgttcgccac
    28321 ctgggagagc gccttttcca gcttctcctg cacactgcgg acttcattct gccgttgcca
    28381 gccgtaatac ttactgccgt cgtactcaat gcccagcgca attttataaa ctggcagttg
    28441 ttgctggtcg gacattagta cagatactcc tgcaccagtt tctcggcgat ttttactgcc
    28501 atcagcgcgc cgccaaagcg aacgttatcg gccaccgacc agaactggac ttgctccggc
    28561 ataccgtagt cattacgcac gcagccaaca gaaagatgcg gcgtacccga agcatcccct
    28621 acctgagtcg ggaattcgtt ctcttcagag agcacaatat cttcgccctg agcaaacgca
    28681 tcacgcgctt cttctgccgc cagcggacgc agtgcttcaa agttgaccat ctgggcatga
    28741 ccgtagaata ccggtgcctg gacgacgctt gccgaaatca tcagcccttc gtcctgcagg
    28801 attttgcgta cttcgtcaac gatacgacgt tcttcacgca cgctaccttc gctatccggc
    28861 aacaacggca gcatattgaa cgccagttga cgaccgaaga aatcttcttc gtcaatcgga
    28921 atgccgttga gcaatttcgc actctgcccc gctaacgcat cgaccgcttt tttgccctgg
    28981 gcggaggctg aaatcaggct ggtaacactg atacgcgaca aaccgccctg atcgattaac
    29041 ggtttcagtg ccgccagcag ctggctggtc agactgtctg gtacggcgat gacattccgg
    29101 ttgcggtaat cggtcagtac aaacgggttt acttccggca ccaccagcgg tacgtcgggt
    29161 tcgagagcaa acagtccgct gctgtcgatc accaggcaac ctgagttggt cgcttcttca
    29221 acccaggcag cggtagcttc tttgcctgcg acaaaaaatg ccagctgcgc ctgcgtccag
    29281 tcgaattcag cggcatcctg cacggtgatt gttttaccac caaagcgcag ttgttcgcct
    29341 gcgctttcgt tacgtgccag tgcataaatt tccccaaccg ggaactgacg ttcagccagc
    29401 gtttcaagca gggcttcgcc catagcgcca gttgcgccca ggacggcaat gttccagcct
    29461 tcagacatgg tggtttactc cagaaatagc aaagctccct gccaatgtta cagcagagag
    29521 catgaagaag agattaacgt gccggatgat gaacggcgtt aaaacccagt ttacacagca
    29581 atgatgccgc actggcgtca tcacaaatta catacagaga tgaccattca cggcgctcaa
    29641 gatagttttt gcgcagttta tcgaactcac ccggtatccc ggcgacttta cgcagcggtg
    29701 cgtcatcgcg gcgcacatca tacaccaaat gcaccagcct tttcagcgtc ggttgatcga
    29761 gcggaccatg cagcgtaatg cggccaaact caggcgcggg cagcaacgta tccagcgcaa
    29821 cgtgttgttc atgcccaata aacttgctat aggcttcaaa cacttgcgtc gtgccacgcg
    29881 ctttaccctc cagggtatag cctgcgatat gcggcgtgcc gatatccact tttgtcagca
    29941 attccacatt aagttccggc tcgccttccc agacatccag caccacgctt aacttctggc
    30001 cttcattcag gcaggtcagc agcgcggtat tatcgacgac tgcgccacgg caggcgttaa
    30061 tcagaatcgc tccgggcttc aggctacgga tcagtttttc atccgccaga tgtagcgttt
    30121 tgtacggacc atctttaaag agtggcgtat ggaaagtcag aatatccgcg cgctggacta
    30181 actcatccag cgagcggaaa tcaccctcat ccccacggtc ggcgcgaggc ggatcgcaaa
    30241 gtaaggtttt aatccctaac gcttccagtc gcgcctgcaa tcgacgtcca acgttaccaa
    30301 cgcccacgat ccccaccgta cggtcgtgca gtgaaaatcc atcgcgttcg gcaagcatca
    30361 gcagggagga gaaaacatat tccaccaccg caatcgcatt acagccaggt gcagcggaaa
    30421 aaccaattcc cgcctgcttc aaccatgctt catcgacatg gtcggtcccc gcagtggcag
    30481 ttccaacaaa tttaatgggt tttcctgcca gcaaagattc attcactttc gtgaccgaac
    30541 gcaccatcag cgcatccgcg tctgccagtt gagcgacggg gattggacgc ccgggaaccg
    30601 cgatcacctc acccaaacgg ctaaataagt cgcgggcata aggcatattt tcatcaacaa
    30661 ggattttcac gtttgtgtta cctgtatgag acgagagtta accggacaag tatgccataa
    30721 tctcgcggcc aggcatactt gcgaagattt caggtataag gatacgtaat gatacaacct
    30781 atttccggcc ctcctcctgg gcagccacca ggtcagggag ataacctgcc gtctggcgcg
    30841 ggcaatcagc ctttatccag tcagcaacgt acttcgctgg aaagcttaat gacgaaagtg
    30901 acctcactga cgcaacaaca aagagcagaa ctgtgggcgg gtatcaggca cgatattggt
    30961 ctgtcgggag attcaccgct gctttcgcgt cacttccctg ccgctgagca taatctggcg
    31021 caacgtctgc tggccgcgca aaaaagccat tctgcccgcc agcttttagc gcaattaggg
    31081 gagtatttac gtctggggaa tcatcgtcag gcggtcacgg attatatccg tcataacttt
    31141 ggtcagacgc cgctgaatca gctctcaccg gagcaattaa aaaccattct caccctgttg
    31201 caggaaggga agatggttat tccgcaacca cagcagcgcg aggcgaccga ccgtccttta
    31261 ttaccggcgg agcacaatgc gctaaaacag ctggtgacca aacttgcggc ggcaacgggg
    31321 gaacccagca aacagatctg gcaatcgatg ctggaacttt ccggggtgaa agatggcgag
    31381 ttaattccag cgaaactgtt taaccatctg gtgacctggc tacaggcgcg tcagacgcta
    31441 agccagcaaa atacgccgac gctggaatca ctacagatgg cgctaaaaca acctttagat
    31501 gccagtgaac tggcggcgtt atcggcatat atccagcaaa aatatggcct ttctgcgcaa
    31561 tcatcgcttt cttctgccca ggccgaggat attcttaatc agctttatca acggcgggtt
    31621 aaagggattg atccgcgtga tatgcaaccg ctgcttaatc cttttccacc gatgatggac
    31681 acgttgcaaa atatggcaac gcgtcccgcg ctgtggatac tgttagtcgc gattatcctg
    31741 atgctggtct ggctggttcg ttaaccccga cgaaatgaca gtatcgtgac aataataccc
    31801 agcaccgcag agatcgcccc ggcaagaaat accgaagagt aaccaaacgt ggtcgccagc
    31861 attcccgcca gcggcccgga gacgccgagg gcgatatcct gaaacgcggc gtaaccgccc
    31921 agtgcggtgc cgcgaacttg tgaggggacg cgtttaacca cctccacgcc cagcgcagga
    31981 aagataagcg aacatccggc tccggttaac gccgcgcccg ctaatgcgac ccatgcacct
    32041 ggggcttgcc agagcagcaa caagcccacc gtttctacaa gcagagagac aatcgccact
    32101 ttcacgccgc caaaacggtc cggcatccag ccaaacatga cgcgcatcac gacaaatgcg
    32161 ccgccaaacg cggtaagagt aaagcccgcc atcgcccatc ctttgctggc aaagtagagc
    32221 gaaacgaaag tcccgataac cgcaaaacca acgccttgta gtgccagccc taaccctggt
    32281 ttccagataa gcccgacaac gctccacagc gatggacgtt ctcccgccag ggccggtact
    32341 ttgcgcactg tgccgttaca ggcccacgcc agtaagggta ataccattgt ggtgatcgcc
    32401 agtgcggcaa aaccgtaatg gctatgaatc aacaggccaa gcggcgcacc aacagcgagg
    32461 gcaccgtaaa tcgccattcc gttccatgac atcactttgc cagagtgttt tggccctacg
    32521 atgcctaacc cccaggtcag agcgcctgtc agtaactggc tttcaccaaa cccaagaatc
    32581 aaacgcccga tgaccaacag ggcaaatttg aacggtgcgg agacaggcaa aatcgccgcc
    32641 agcagcaacg cgccgccagc cagaccacaa gctaacatcc cctgaagcgc cgaacgtttt
    32701 gcgccatatt gatcggccag tcgcccggcg taaccacgcg tcagcaccgt agccagaaac
    32761 tgaatcccga cggcaatgcc gaccatggta ttgccatagc ccagatcatg atgaacaaac
    32821 agcgggataa ccggcaacgg caaccctacg gtcatgtagg tgagaaaaac cgcaaaagag
    32881 atgcggaaga gcgaaaaact ggcagaagat cgtgtttcgg tttggcttac agcagtcatg
    32941 cattactcca gaatgcagcg caaggcgagg agtatccccg tctcatctct ctggtttcag
    33001 ggttacggtg cgttggcagg atttaacgcg tacgtctttt cagaaggaaa tcgacaaagc
    33061 gggaagtttg cctagaactg gtggcgattg tcaatgatgt gaaaaaggga accatcaggt
    33121 tcccttttgc gttagtgccg gaggccgcat tggcgcgtaa cgtcggatgc gacgctggcg
    33181 cgtcttatcc gacctactcc ggcctactgc gaattaatct tttcagcttg cgcattacca
    33241 gcgtggcgtt ggtgccgccg aagccgaagc tgttagacat aacagtggtc agttcgcgat
    33301 cggtcgtttc ggtcacgatg ttcagacccg cagcctgctc gtccagctct tcaatgttga
    33361 tgctcggggc gataaagccg tgttccagca tcagcagaga gtagatagct tcctgcacac
    33421 ccgcagcgcc cagtgagtga ccggtcatgg ctttggttgc agaaatcgcc gggctcttat
    33481 cgccgaacac ttcacggata gctgccagct ctttcacgtc gccaaccgga gtcgaagtac
    33541 cgtgagagtt caggtaatcg attggggtat caacgccatg catcgccatc tgcatacaac
    33601 gtactgcgcc ttcgccagac ggagcaacca tgtctgcacc atcagaggtt gcgccgtagc
    33661 caacgatttc agcatagatg taagcaccac gcgccagcgc gtgttccagc tcttcaacca
    33721 ctaccatacc gccgccgcca gcgataacga aaccgtcacg gtgagcgtcg taagtacggg
    33781 aggctttttc cggggtgtcg ttgtatttag tagatagcgc acccatcgcg tcgaattcgc
    33841 aggccatttc ccagcacagc tcttcgccgc cgccagcaaa cacgatgtcc tgtttgccca
    33901 gttggatctg ctctactgcg ttaccgatac agtgtgcgga agtcgcacac gcggagctga
    33961 tggagtagtt aacgccatga attttaaacg gcgtggcgag acaggcagaa acgccggatg
    34021 ccatcgcttt agtgaccaca tacgggccaa ccgctttcag gccgcgcggg ccgcgcattg
    34081 cgtcagcgcc gaacacctgg aaacgcgggg aaccgccgcc ggaacctgca atcaggccaa
    34141 cgcgcgggtt attctggtaa gcttccggag agaggcccgc atcagcgatt gcctgctcca
    34201 tagaaaggaa tgcataaatg gatgcgtcgc tcataaagcg cacaactttg cggtcaatga
    34261 ggccagtggt atccagtttt atgttgcccc agacgtggct acgcatgccg gaatccttca
    34321 gctcctgaga gaaagtgatc cctgaacgtc cttcacgcag agatgccagg acttcctgct
    34381 ggttattacc gatgctggaa acaatgccca ggccagtaat cactgcacgt ttcattcaat
    34441 acctctgtaa gtcgcacata gagtaagttt cgaatgcaca atagcgtaca cttgtacgcc
    34501 gaacaagtcc gatcagccat ttaatagaga tatttgcgca gccttacaca catcgctaag
    34561 atcgagccac cgcctgtaag acgagtaact tacgtgaaac actactccat acaacctgcc
    34621 aacctcgaat ttaatgctga gggtacacct gtttcccgag attttgacga tgtctatttt
    34681 tccaacgata acgggctgga agagacgcgt tatgtttttc tgggaggtaa ccaattagag
    34741 gtacgctttc ctgagcatcc acatcctctg tttgtgatag cagagagcgg cttcggcacc
    34801 ggattaaact tcctgacgct atggaaggca tttgatcagt ttcgcgaagc gcatccgcaa
    34861 gcgcaattac aacgcttaca tttcattagt tttgagaaat ttcccctcac ccgtgcggat
    34921 ttagccttag cgcatcaaca ctggccggaa ctggctccgt gggcagaaca acttcaggcg
    34981 cagtggccaa tgcccttgcc cggttgccat cgtttattgc tcgatgaaag ccgcgtgacg
    35041 ctggatttat ggtttggcga tattaacgaa ctgaccagcc aactggacga ttcgctaaat
    35101 caaaaagtag atgcctggtt tctggacggc tttgcgccag ctaaaaaccc ggatatgtgg
    35161 acgcaaaatc tgtttaacgc catggcaagg ttggcgcgtc caggcggcac gctggcgaca
    35221 tttacgtctg ccggttttgt ccgccgcggt ttgcaggaag ccggattcac gatgcaaaaa
    35281 cgtaagggct ttgggcgcaa acgggaaatg ctttgtgggg tgatggaaca gacattacca
    35341 ctcccctgct ccacgccgtg gtttaatcgt acgggcagca gcaaacggga agtagcgatt
    35401 atcggcggtg gtattgccag cgcgttgttg tcgctggcgt tattacggcg cggctggcag
    35461 gtagcgcttt attgcgcgga tgaagccccc gcgctgggtg cttccggcaa tcgccagggg
    35521 gcgctgtatc cgttattaag caaacacgat gaggcgctaa accgcttttt ctctaatgcg
    35581 tttacttttg ctcgtcggtt ttacgaccaa ttacccgtta aatttgatca tgactggtgc
    35641 ggcgtcacgc agttaggctg ggatgagaaa agccagcata aaatcgcaca gatgttgtca
    35701 atggatttac ccgcagaact ggctgtagcc gttgaggcaa atgcggttga acaaattacg
    35761 ggcgttgcga caaattgcag cggcattact tatccgcaag gtggttggct gtgcccagca
    35821 gaactgaccc gtaatgtgct ggaactggcg caacagcagg gtttgcagat ttattatcaa
    35881 tatcagttac agaatttatc ccgtaaggat gactgttggt tgttgaattt tgcaggagat
    35941 cagcaagcaa cacacagcgt agtggtactg gcgaacgggc atcaaatcag ccgtttcagc
    36001 caaacgtcga ctctcccggt gtattcggtt gccgggcagg tcagccatat tccgacaacg
    36061 ccggaactgg cagagctgaa gcaggtgctg tgctatgacg gttatctcac gccacaaaat
    36121 ccggcgaatc aacatcattg tattggtgcc agttatcatc gcggcagcga agatacggcg
    36181 tacagtgagg acgatcagca gcagaatcgc cagcggttga ttgattgttt cccgcaggca
    36241 cagtgggcaa aagaggttga tgtcagtgat aaagaggcgc gctgcggtgt gcgttgtgcc
    36301 acccgcgatc atctgccaat ggtaggcaat gttcccgatt atgaggcaac actcgtggaa
    36361 tatgcgtcgt tggcggagca aaaagatgag gcggtaagcg cgccggtttt tgacgatctc
    36421 tttatgtttg cggctttagg ttctcgcggt ttgtgttctg ccccgctgtg tgccgagatt
    36481 ctggcggcgc agatgagcga cgaaccgatt ccgatggatg ccagtacgct ggcggcgtta
    36541 aacccgaatc ggttatgggt gcggaaattg ttgaagggta aagcggttaa ggcggggtaa
    36601 tctgctctcg cagtgtttgc cggatgcggc gtgaacgcct tatccaacct acgtgtgacg
    36661 tgtaggcctg ataagacgcg ataagcgtcg catcaggcat ggtgctccag acgccgcaac
    36721 ttacagctgt gacgcctgct gaaacaggtt ttcccacata tcggtcacca gcgcctggtc
    36781 acgcggcgac aattcaccgg caccaatggc tttttccaga ctctggctaa cggtcgtatg
    36841 caccgcctga gcggagtggt catcaccact ttccagttct gcgatggcta atgtcaggtg
    36901 gccacgcaaa tacccactgg caaacaactc atcatcactg gcatggtcaa ccatgccgtc
    36961 gattaatgcc agaatgcgtg attcaaactc cgcgatcatc ttctttcctc attgtaaaac
    37021 gtggcgctgg cttcagttga gcgcttccgg ccacgggaac tgttccgccg taagttccgg
    37081 cgtgtgataa taattttgta gtgctttaat gaagcgtgcc gggcgttccg gaatgccatt
    37141 tgtcagataa tccatcacct gcgcatgtac ccgccgttga aacaccacac gatccggttc
    37201 gaaatcccct tccagattgt cgcaactgac gttaaacgga tatccagccg ccacgcagaa
    37261 taaccagtcg aacgcctgcg gcttcacttc aacatcttca aactggcttt gcgtctgggc
    37321 atcgcgtccg tccgggcaat accagtagcc gaaatcaacc agctcacggc gcgcttttcc
    37381 ggcaatacac cagtgcgaaa tctcatgaat tgcgctggca taaaagccat gagcaaagac
    37441 gattcggtta tacggtactt ccgcatcagc aggaagatag atcggttcgt cgtcgccttt
    37501 aatcagacgg gtattaaatt catcggcaaa gcagctatta aaaatttcaa tcaactgctc
    37561 gtagtggtgt gtactgttca ttagttcatc cccaaccagt ggaggatctc ctgtccgtgg
    37621 ctatcgtaaa gtaatttggc actcatcacc gccgagacga taacaatcat cgggcggatc
    37681 agcttttgtc ctttgctcaa caccaaccgt gaacccatgc gcgcgccgag gaactgcccc
    37741 accagcatga caaagcccgt cgcccaaatc actttgccgc cgagaataaa cagcagcaga
    37801 ccgccaatgt ttgacgttgc gttgagtaat ttggcatgag ccgtggcttt ggcgaggttg
    37861 aatccgcaca gcataacgaa ggccagcgcg taaaacgatc cggcagccgg gccaaagaat
    37921 ccgtcataaa aaccgacgca gccaccggca attaacgcga agggtaaacc gtacatccgg
    37981 cgctggcgat cttcttcacc cagctttggc atcagcaaga aatagaggcc gatacaaatc
    38041 accagaatgg gcaaaatctg ccgtaagaca tcagcctgaa cgtactgtac caacagtgcg
    38101 ccgctcattg agccgacaaa ggtcatggca atattgagtt tctgatcgct taaactaacc
    38161 actttgcggc gaataaagta gatagtggcg gaaatagagc cgccgcaggc ttgcagttta
    38221 ttggttgcca gcgcgttagc gggagacatt cccgcagcca tcaatgccgg aatggtgagt
    38281 aacccaccgc caccggcaat cgagtcgata aatcccgcca gcatggcgac aaaaaagaga
    38341 actcccagca acagcggaga aaccataaac aggctattaa acgtttccat tagatcacgt
    38401 gctcatccag tagcgcctgg caggaaggcg gcaacggagg cggtgtcttc ttctcaggct
    38461 ttgttgttcc cggttttgga ggttcaaacc agctttgcag ttctgccccg caaccatcgc
    38521 ctgatggcgg taaaggttga tcttcacact ccagactatc ggcagggcaa cgtaatcgta
    38581 catgcatatg cgcgcgatgc tggaaccagg gtcgcacttt gcgcaaccag tcgcgatcgg
    38641 tgcccgcatc aaggcaaagt tgttgtttaa tcgccggatt aacaaaaatg cgcgtgacgt
    38701 ctttgtcctg ggcggcgagt ttgatcaagc tgaaaatttc tggcttccac agcgtggaga
    38761 caacgtgttt accgtcgcgg gaaactaagt ccagtgcttg cgggcgcaag agctgcgcgg
    38821 aggtccagcg agttttcggc agttgcagaa agatatcgac atccagtccg gtctggtggc
    38881 tggcatgacc gccgttgaaa cgcccaccag cgggcatccc catatcgcca atcagcaccg
    38941 tacccatgcc cagattgctc acctggctac tcagacgctg gataaacatc accagatccg
    39001 ggtgaccgaa atagcgacgc tgatcggtac gcatgacctg ataatgttcg gactgtatcg
    39061 gcaacgtgtc agcgccgaca atacagccat tagaaaaact gcctatcgat tgtgcgctac
    39121 ccggcacagg ttgggttatt ttttgccacg gcgtcgctgc caggctagca ctactggcaa
    39181 gcagagccag cagcgcaatc gcggttttgt tcatttttta ccagcgtgga atatcagtct
    39241 tcacatcggc attttgcgcc cgctgccgta acaggtgatc cattaaaacg atcgccagca
    39301 tcgcttcagc gatcggcact gcacggatcc cgacacaggg atcgtgacgg cctttggtga
    39361 tcatctcaac ttcttcgcca aagcggttaa tagtgcgccc cggcacggta atgctggagg
    39421 tcggtttcag cgccatatga gcaatgattt gctgcccgct gctgataccg ccgaaaatgc
    39481 cgcccgcatg gttgctctgg aaaccgtctt tggtgatttc gtcgcggttc tggctgccgc
    39541 gcagcgccac tacgtcaaaa ccgtcgccaa tttccacgcc tttcaccgcg ttgatgctca
    39601 tcagcgcatg ggcgatgtcg gcatccaggc ggtcaaagac cggctcgcca agtccggcgg
    39661 ggacaccact ggcaacaacg gtgactttcg cgccgatgga gtcgccctct tttttcagcg
    39721 cgcgcatcag ttcatctaac gcgtcgattt tgtcggggtc cgggcagaag aacggatttt
    39781 gttcaaccag cgaccagtct ttgatttcca gcggaatgtc gcccatctgg gtcaggcagc
    39841 cgcggatttc aataccaaat ttctcggcga gatatttttt ggcaatcgcc cctgccgcca
    39901 cgcgcatggc ggtttcgcgg gcggaagaac gtccaccgcc gcgataatcg cgcagaccgt
    39961 atttttgttc gtaggtgtaa tcagcatggc ctggacggaa gacgtcttta attgcgctgt
    40021 aatcctgaga acgctggtcg gtattttcga tcaacaagcc aatgctggtg ccagtagtaa
    40081 cgccttcaaa aacaccggag agtattttga cctgatccgg ctcgcggcgc tgggtggtat
    40141 agcgcgatgt cccagggcga cgacgatcaa ggtcatgttg caggtccgct tccgtcagcg
    40201 gaatgcctgg cggaacacca tcgacgatgc agccgagcgc cagcccgtgc gattcgccga
    40261 aggtggttac gcgaaagagt tgtccaattg tgtttccagc catcacggct ccgttattgt
    40321 tgtgtttgcg tgtttactta atctttataa atcgcgaaat gttctcgtgc ggcaataagc
    40381 tgctctttag tgagcataaa cacaccatcg ccgccgttat caaactccag ccaggtgaac
    40441 ggaacatcgg gatattgttc cataagatgt accatgctgt tgcccacttc acaaatcaac
    40501 acgccatcat cagcaaggta atctgccgcg ttaccgagaa tgcgacgcgt cagtttcagg
    40561 ccgtcggtgc cagatgccag gcccagttcc ggctcgtgac ggtattcgtt tggcaggtcg
    40621 gacatatctt ccgcgtcgac atacggcggg ttagtgacaa tcagatcgta ttgcactttc
    40681 ggcaaatcac ggaacagatc ggaacgaatc ggaatgacgt tgtggatcag accgtgttct
    40741 tcgatgttct gttcggcaac cgccagcgcg tcgggagaga tatctaccgc gtcgacttcc
    40801 gcttccggga aggcataagc acaggcaatg gcgatgcagc cgctaccggt acacatatct
    40861 aaaatatgct gcggttgctt gctgatgagt ccggcaaatt tattgttgat cagttcacca
    40921 atcggcgagc gcggcaccag cacgcgttca tcgacgtaaa attcatgacc gcagaaccag
    40981 gctttgttgg tcaggtaagc caccggaatg cgctcgttga cgcggcggat cacgcgttca
    41041 acaatacggt gtttttcgct ggaggtcaga cgcgcggtgc gcatatcttc cgggatatcc
    41101 agcggcaggt agagcgatgg taacaccagc tgtacggctt catcccacgg gttatcggtg
    41161 ccgtgaccgt accagatatt tgccgcgctg aagcggctta ccgaccagcg caatatgtcc
    41221 tgaatggttt gcagctcatt tactgcttca tcaacgaaaa ttttatccac gtattcctcc
    41281 agggcatgat cgcaataatt tcggcggcta gtttgccatg aagatgacga taaatcagca
    41341 ttcacgcgcg gtgagtgagg aaaaatacgt ttaaaacgat cgattgtgct acgagtcggg
    41401 taaactgtag gaaaattaga aatagagaca gataaatgaa aaagaaaaca acacttagcg
    41461 aggaggacca ggctctgttt cgccagttga tggcggggac tcgcaagatt aagcaggaca
    41521 cgattgtcca ccgaccgcag cgtaaaaaaa tcagcgaagt gccggtgaaa cgcttgatcc
    41581 aggagcaggc tgatgccagc cattatttct ccgatgagtt tcagccgtta ttaaataccg
    41641 aaggtccggt gaaatatgtt cgcccggatg tcagccattt tgaggcgaag aaactgcgcc
    41701 gcggcgatta ttcaccggaa ttgtttttgg atttacacgg tctgacgcag ctgcaggcca
    41761 agcaggaact gggcgcgttg attgccgcct gtcgccgcga acatgtgttt tgtgcctgcg
    41821 tgatgcatgg gcacgggaag catattttga agcaacaaac accgctgtgg ctggcacaac
    41881 atccgcatgt gatggcattt caccaggcac cgaaagagta cggcggtgat gcggctttgt
    41941 tggtgttgat tgaagtggaa gagtggttgc cgccggagtt gccctgaatg ctggcagcaa
    42001 atgccggagc agcgtaaacg ccttatccgg cctacaaata tgtgataatt caataaattg
    42061 caattcactt cgtaggcctg ataagacgca tcagagcaat actacgaact tcagttcatt
    42121 caatacatac tgaaaacgac acgtcgaatc aatcggttgc ccactactgg tattcacatt
    42181 caccacaata tcgtgtagta ctttatccgt taccggatga cgagttggcg taaccgttac
    42241 cgttgattta tcacaaccgt aagaggtaaa ggttaacaat tctgataccg cgcggcagta
    42301 tgctcctctg ccatcattat cagaaaaatt actgccatca cagagtctgc cgccttccat
    42361 caggtaggtc gaaagcgtgg tggtcactgt tgaccccgtg gactgaattc gtaacgcctt
    42421 attatggctg acataatttt cactccagga gatatcgtat gaagcgaccg gtatcgttgg
    42481 gcatattgtg ttatgcactg taggggccgt atagcttcca aatccgggta aatctgtata
    42541 ggaataaaag gatgcgatcc aagggcaacc ataatcatca gatgcaacgg taattttaaa
    42601 cgtcatatat gcacaacgaa aaattttcgt atctatgcca gggcaatttg agttattaat
    42661 tgtatcaaag tcagtcagta ccaaagttgg agctgaaccc gacccagacc aggaaataac
    42721 ccgcaacgta tcgcctttat acgcaaaaga atcatatttc ccatatgcaa cctcctttgg
    42781 cgttatcacc gtaacattga aaacactgct cgctacagca ccggttacct tgtccatggc
    42841 ataataaact ctcacactgt caaaactcgc actcataagt ttatctccag cataactctt
    42901 agatgcgccg acaacactca gaacgagcag cattaggctc gcgaaaatta tggtccattt
    42961 tttcatttca gaaatactca aaggttgccg tcactgtggc actaaagtct ccggacgtta
    43021 catctcgccc gctctttgcc tgtaaccagg tattaaaatt taggctgtta tttccctgat
    43081 tcagcgggag cgtgacgcct gttgtattgt tgattggcac cgatgtgcct gccgcagttt
    43141 caataccaat cccagcgccg gatgctgatg acgaggagtc aaaggccaga aagccgggta
    43201 gagcactatc ttctgttcct gtcaacgtta ctttgacatt aaacaatgta gaactatgac
    43261 aatctttcaa ttgaaacacc aatggcactc gctctgactg cccaaggttc attaaatcac
    43321 tggcggcaat ggcaggaaaa tgtacttctg ccaactggct accattaatc actggcgtac
    43381 acgatttact gattaaattg ccggtaaagt gcaaattatc tcctgctatt gtggttccac
    43441 tgcccagcaa catcaggcta ctaataaaaa acaacgtacg ctttttcttc attggtaatc
    43501 tacctgtaac gtggcccagg cctcaaagtc cgcctctggc agaatgacac cactcttttt
    43561 tactggcaca gccgttaaca ccggtaaatc ggtttcattc accacaagtg gcgtattgat
    43621 cgtaaaactc tgccctgcct gtttgagctg aatgcctaaa ccagtgatat tgctgcttac
    43681 tgcagcatta tcaaaatcgc ttggtgtgcc actccagctc aaggtcattt ccattaatgg
    43741 gtcgcgaacc gccgtatcgc aggtaatgga atacggcacg gtttgctcat aattgctgcc
    43801 attaatgtca tcaataagca catcctgaaa agttatttca atggtttgag cgttgctaat
    43861 cgtgcagtta ggcggctgca ctaaataacc gtgaaaagag acgtttgaga gtgccatcga
    43921 gcaaaagccc cacagcaaaa taagcgagat ccgtttcatt ggtaatccac cacaattgtg
    43981 gcactggcgt taaattcagc tgccgctaac gttgtaccac tctgtttcac cggcacagct
    44041 tccagaacgg gaactcccga ggagaaatta aatggcaacc atgagccaga ggttaagtcg
    44101 agtattgtgt gatcactact tttctgaacc cgaataccaa acccaggaat acccgtgctg
    44161 ataactgttt caccgttaat cacgaccgtt gtcgcctgca tctgtagacg caggtcatcc
    44221 attgccagat tgttacaaac aagcgagtag tcaatgggac gtttataatc aaccctattg
    44281 atcttattaa tgaatacatt accaaactca actgccgcac catttaccgt acaaggagga
    44341 ggggcatcga caagcacccg taaattcata cttactgact tactcgctgc aactgcaaag
    44401 ccgctaccaa cacaacatat cagtaaccca gttaccgccc agacagaaca agatcctttc
    44461 atactggctc tcccggcctc agcctttgcg gctctgctct tcatcgacct agcaactgtt
    44521 accgttgcaa gtgaaaaaga gcggcatccg agcgccatag tcgttgacat aggtcagtac
    44581 cggcacgcta cccatcttca cattcagcgg tgcagaggtt ttcggcgaca tcactattgg
    44641 cgagaaaccg gctgccggat tgccattttt ctgtgtgctg gcattactga aaatgacgta
    44701 atacgacgtc gggttattga cggtaaattc ctgaccgttg cgggttaaag taatcttata
    44761 ttgctagggg tttttcatgc tgacgttctc cagcgccttc ggtcgccaga acaacttaat
    44821 acgcgtttgt agtgctattt gcagcgtgtt ggctttgttt gattttggcg ggatctcacg
    44881 cacgttgaag tagaacaggc tttcacgatc ggcaggcaat ttgttgatat ccggcattcc
    44941 ctgcactttt acctgcccgt tcatcattga atcgatgcgt tgcaccggtg gcagaacagt
    45001 taacggtgaa ctgatcttgt tacccttttc atcttcgatc cagctttggg ccagataagg
    45061 cagtttcgga tcgttattgc gcaacgtcac gctgattgat ttgtcgcttt cattaaatat
    45121 caaacgggta cgatctggcg ttacagaagc ctgggcagac aaactggcag cgctaagcag
    45181 taacgctagc gtcgtagcac ccaatttggc agagcaaagt aaatcagaca tggatcattc
    45241 cttaatcacg ttattggtta gctgaaacag aaacaggggg attggtgggt gtcacctgtt
    45301 gcgtctgctc ctgaatgaca ggctttatgt catcaggaac aaccggcgcg acgttgcctt
    45361 tatgctggca tggcagtaac aagccgttaa acagatcggc aggcagcggg tccggcaggt
    45421 tgatatcgca atgcgcaaca ccactccaga acacactcat gtgttcgcca ggtttcactc
    45481 cagccagata aacattgccg tcatcatcga caaggcccac tgtctgctcg ttatcatttt
    45541 ttacttctgc gccaaacgga ggatggctgc cgtcttgtaa acgcagcacc gccatcgctt
    45601 tttgaccact gatgacggca aatttgcggt agccaatcgc cccttcagtt agcgtggctt
    45661 gtaccaccga ctgggttgct tcggcgtttt ccggcagttt gttgaggtcg atatacgcct
    45721 gattgcggta atagttattg acgtcagaaa cgacggcttt accaaacata ttggtataaa
    45781 cagcagcacc gttaccttct accggaacat cggcaacgcc atcggcatca atcaacaagc
    45841 gtgtcccgcc catattctgg gtacggtgaa gtgcgccacc gtgggcagta agcgttgcgc
    45901 cgccctgtaa cgacaagccc gcagaggtgt attgcccttc atggtagttc gcactgaggt
    45961 caacctgcgc cagcgaacca tcatggctgt aatagccgtc aacgctggtg tgtttgtcac
    46021 tggtgccgac gttcaactga tagtgagtcg cgtcatcgac acggctgaaa taaccgacct
    46081 gactgctgtc cgtcccactg ccatagtacc gttatagcta acggtactgt tgtcgcccca
    46141 cggcatactg agcgaaatgt acatgccttt gtcggcctgg ttgtcatact cgtaacggta
    46201 gccagtcatc gagatgctga cattacgaat actgcccata ttgaaatagt gcgagagcat
    46261 gatgttgtag tttgtctgct cctcgcgatc ccagtaggta tggcgggtat agttgagata
    46321 aaccgaaaca cccgcatcgc ggaagttctg gttataagtg gcggtgtaca tctctttgtc
    46381 gttgcccgtg cgtaccattc cgctgtcact ggcatccaga tactcgctca tggtcataaa
    46441 gttctcttcc gagaagcgat atccagcaaa agtaacgcgg ctgttgagct ggtcaaagtc
    46501 tttggaataa ctcacacgga aggagttacc gtccagcgaa cctttgccat aagcggtgtc
    46561 tttatccagt ttggtgtgcg agtgagtaac atcaaacgcg accgcgccga atgtagacaa
    46621 atcgcgaccg acaccaagcg ccgcagactg atagttttca tctcccagtg cgccgccata
    46681 taacgaccag ccgttagcga tcccccagga agcttcagca ccagaaaaaa attcaccctc
    46741 gacatggtgt ccccactctt gcggacggcc catcatgatc ttatagcgaa cctgacctgg
    46801 acgagtgagg tatggcatcg aggcggtgct gatgtcatat tcctgcacct ggccgttctg
    46861 ttcttcaatg cgaatatgca acgtaccgga gacagaatca ccaagatcct gaatacgaaa
    46921 cggtccagcc ggaacctgcg tttcgtaaat cacacgcccc atctggctga cggtcacttt
    46981 tgctgtggtg tgtgccacgc cggaaatgtc tggcgcgtag ccgcgcagat tgggaggcaa
    47041 catttgatcg tcagtactga cactgccacc aacatagtta aaaccatcaa aaatatcgga
    47101 tctgaggtaa tcctcgccca gcgccagttt ggcttttaat gatggtaacg cccgcctggc
    47161 atagtagcga ctccactccc atttttttga gtttcatcgc cgctgaattc atcgtcatca
    47221 ttactgcgag tatgttgata gttagtctgc cagtcagcac gcatacgcca cggccctagg
    47281 ttaaccccaa ccgtcccgtt gccgctgatc tcgttactat catcaccgcc attttcttcg
    47341 tgccgggttt gtgcgttgat gctgtagtcc gcgacgatcc cggagatgcc gtcatcccaa
    47401 cgtgaaggcg gatccctgtc gggataagta tattcgaggt aagcctgtgg cagtgaaatg
    47461 actaatgcgg actggcttaa atcagcctta atttccatgc cttccagttg accggatttc
    47521 aggcatttag catcgtggct ccattgcaga tttttcgcca cgtcttcttt taaaccaaac
    47581 tgcgctacca gttccggtgt cagacaagca tagcttttgc tcgcgtcatc ttcaccagca
    47641 taccagtaaa tatcgtactc ttccgccaat ggctgtttat ttagttgaac ctgtaaattg
    47701 tattttccgg gctcaacata tccctggctg gaaaaacgct tcatatcaat ttttgtgtcg
    47761 ccttttaatt ccagaaaacg ggaatcaaac tgaatgtcgt cttcagccca gacactgcta
    47821 taactccctg acattgccag cgcaatgcac cacggaagaa tccgtaatcg aaaaagagaa
    47881 tggtcaggca tacacgtttc tatccgtttt tattcttgtt cggtcaaaat acatttaaat
    47941 cccccatagc agtgtcgcta tgggggtatg ccttacaggt aagtaatctg gaaggtggta
    48001 tttgcttcaa aattacccag atctggcgca tcagctgcgc cgaccagcca ggcgttaaag
    48061 ttcagtgtct gcttcgcttt gcctttgttg gtagaagtgt ctttcactat tttctggtca
    48121 atacccatgc cgcttttgta agaggtaccc agagagtcac cgatcgccag gctaacattg
    48181 ttaaatgccg caccggtatc ggtattgaaa atggtgtaat aattgccgct atttgcagaa
    48241 gaaacggtac cagtaaaggt agtggtcatt gtttcctgag tatcaaatac gcaatcttgc
    48301 agacgaatct ggaaactttt cggcgtggtg gtgccgttat ttttcagttt ttcggcaccg
    48361 atatcaccca tatcaacttc cagtttgtga tcgtccggaa cgatagaaca cactgagtct
    48421 tcaatggtgc cgtaaaaacg tgcagtaccg ttgttacccg cagcgattgt tgccgtagag
    48481 gtgaacacgc ccatcaccat tgccgcagca atagctgttt taacaaactt actcattatt
    48541 tgtccttaaa ctataaataa ttaaaaatat aaacattgca atacattgat tcagtcaata
    48601 gccaatgttt atacccagcc attgaatacg gcatgcgacc caaaaagaac aggtcgtaga
    48661 aaattgtatt cattggtgtg gggccgcata gtgatgtttc cacacccctg atccaattta
    48721 attttactaa agagtaaatg tagatttaat taatatattg acagaagaaa ggttatctta
    48781 agga
//
LOCUS       DAZPAB010000008        48025 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZPAB010000008 DAZPAB010000000
VERSION     DAZPAB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.008"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(97..3246)
                     /gene="acrB"
                     /locus_tag="ACHUPG_000416"
     CDS             complement(97..3246)
                     /gene="acrB"
                     /locus_tag="ACHUPG_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR0860175.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3269..4462)
                     /gene="acrA"
                     /locus_tag="ACHUPG_000417"
     CDS             complement(3269..4462)
                     /gene="acrA"
                     /locus_tag="ACHUPG_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR0860176.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4604..5251
                     /gene="acrR"
                     /locus_tag="ACHUPG_000418"
     CDS             4604..5251
                     /gene="acrR"
                     /locus_tag="ACHUPG_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR0860177.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5379..8741
                     /gene="mscK"
                     /locus_tag="ACHUPG_000419"
     CDS             5379..8741
                     /gene="mscK"
                     /locus_tag="ACHUPG_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR0860178.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8953..9114)
                     /gene="rsmS"
                     /locus_tag="ACHUPG_000420"
     CDS             complement(8953..9114)
                     /gene="rsmS"
                     /locus_tag="ACHUPG_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR0860179.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(9128..9655)
                     /gene="priC"
                     /locus_tag="ACHUPG_000421"
     CDS             complement(9128..9655)
                     /gene="priC"
                     /locus_tag="ACHUPG_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR0860180.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9725..10102
                     /locus_tag="ACHUPG_000422"
     CDS             9725..10102
                     /locus_tag="ACHUPG_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR0860181.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10255..10806
                     /gene="apt"
                     /locus_tag="ACHUPG_000423"
     CDS             10255..10806
                     /gene="apt"
                     /locus_tag="ACHUPG_000423"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR0860182.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10935..12860
                     /gene="dnaX"
                     /locus_tag="ACHUPG_000424"
     CDS             10935..12860
                     /gene="dnaX"
                     /locus_tag="ACHUPG_000424"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR0860183.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    12193..12257
                     /gene="dnaX"
                     /locus_tag="ACHUPG_000424"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12913..13242
                     /locus_tag="ACHUPG_000425"
     CDS             12913..13242
                     /locus_tag="ACHUPG_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR0860184.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13242..13847
                     /gene="recR"
                     /locus_tag="ACHUPG_000426"
     CDS             13242..13847
                     /gene="recR"
                     /locus_tag="ACHUPG_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR0860185.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13957..15831
                     /gene="htpG"
                     /locus_tag="ACHUPG_000427"
     CDS             13957..15831
                     /gene="htpG"
                     /locus_tag="ACHUPG_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR0860186.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16012..16656
                     /gene="adk"
                     /locus_tag="ACHUPG_000428"
     CDS             16012..16656
                     /gene="adk"
                     /locus_tag="ACHUPG_000428"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR0860187.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            16788..17750
                     /gene="hemH"
                     /locus_tag="ACHUPG_000429"
     CDS             16788..17750
                     /gene="hemH"
                     /locus_tag="ACHUPG_000429"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR0860188.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17747..18706)
                     /gene="aes"
                     /locus_tag="ACHUPG_000430"
                     /pseudo
     CDS             complement(17747..18706)
                     /gene="aes"
                     /locus_tag="ACHUPG_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            18858..20162
                     /gene="gsk"
                     /locus_tag="ACHUPG_000431"
     CDS             18858..20162
                     /gene="gsk"
                     /locus_tag="ACHUPG_000431"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR0860189.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(20295..21971)
                     /gene="ybaL"
                     /locus_tag="ACHUPG_000432"
     CDS             complement(20295..21971)
                     /gene="ybaL"
                     /locus_tag="ACHUPG_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR0860190.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22209..23429)
                     /gene="fsr"
                     /locus_tag="ACHUPG_000433"
     CDS             complement(22209..23429)
                     /gene="fsr"
                     /locus_tag="ACHUPG_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR0860191.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23647..25299
                     /gene="ushA"
                     /locus_tag="ACHUPG_000434"
     CDS             23647..25299
                     /gene="ushA"
                     /locus_tag="ACHUPG_000434"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR0860192.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25336..25815)
                     /gene="ybaK"
                     /locus_tag="ACHUPG_000435"
     CDS             complement(25336..25815)
                     /gene="ybaK"
                     /locus_tag="ACHUPG_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR0860193.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(26019..26813)
                     /locus_tag="ACHUPG_000436"
     CDS             complement(26019..26813)
                     /locus_tag="ACHUPG_000436"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR0860194.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            26951..27292
                     /locus_tag="ACHUPG_000437"
     CDS             26951..27292
                     /locus_tag="ACHUPG_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR0860195.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27507..30011)
                     /gene="copA"
                     /locus_tag="ACHUPG_000438"
     CDS             complement(27507..30011)
                     /gene="copA"
                     /locus_tag="ACHUPG_000438"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR0860196.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30273..31205
                     /gene="glsA"
                     /locus_tag="ACHUPG_000439"
     CDS             30273..31205
                     /gene="glsA"
                     /locus_tag="ACHUPG_000439"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR0860197.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31208..32500
                     /locus_tag="ACHUPG_000440"
     CDS             31208..32500
                     /locus_tag="ACHUPG_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR0860198.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            32531..32628
                     /locus_tag="ACHUPG_000441"
                     /pseudo
     CDS             32531..32628
                     /locus_tag="ACHUPG_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32625..33032
                     /gene="cueR"
                     /locus_tag="ACHUPG_000442"
     CDS             32625..33032
                     /gene="cueR"
                     /locus_tag="ACHUPG_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR0860199.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(33033..33491)
                     /locus_tag="ACHUPG_000443"
     CDS             complement(33033..33491)
                     /locus_tag="ACHUPG_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR0860200.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(33488..34405)
                     /locus_tag="ACHUPG_000444"
     CDS             complement(33488..34405)
                     /locus_tag="ACHUPG_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR0860201.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            34551..35228
                     /gene="fetA"
                     /locus_tag="ACHUPG_000445"
     CDS             34551..35228
                     /gene="fetA"
                     /locus_tag="ACHUPG_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR0860202.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            35215..35994
                     /gene="fetB"
                     /locus_tag="ACHUPG_000446"
     CDS             35215..35994
                     /gene="fetB"
                     /locus_tag="ACHUPG_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR0860203.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(36057..36911)
                     /gene="cnoX"
                     /locus_tag="ACHUPG_000447"
     CDS             complement(36057..36911)
                     /gene="cnoX"
                     /locus_tag="ACHUPG_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR0860204.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(36972..37781)
                     /gene="ybbO"
                     /locus_tag="ACHUPG_000448"
     CDS             complement(36972..37781)
                     /gene="ybbO"
                     /locus_tag="ACHUPG_000448"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR0860205.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(37771..38393)
                     /gene="tesA"
                     /locus_tag="ACHUPG_000449"
                     /pseudo
     CDS             complement(37771..38393)
                     /gene="tesA"
                     /locus_tag="ACHUPG_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            38364..39050
                     /gene="ybbA"
                     /locus_tag="ACHUPG_000450"
     CDS             38364..39050
                     /gene="ybbA"
                     /locus_tag="ACHUPG_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR0860206.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQLFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            39047..41462
                     /gene="ybbP"
                     /locus_tag="ACHUPG_000451"
                     /pseudo
     CDS             39047..41462
                     /gene="ybbP"
                     /locus_tag="ACHUPG_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
     gene            complement(41680..41835)
                     /gene="dinQ"
                     /locus_tag="ACHUPG_000452"
     CDS             complement(41680..41835)
                     /gene="dinQ"
                     /locus_tag="ACHUPG_000452"
                     /inference="COORDINATES: protein motif:HMM:NF041472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR0860207.1"
                     /translation="MKKIIVIGNVRVSCDCLSPLREQRMKTFLDKVLIVLKALIALLE
                     LIRQFIE"
     gene            complement(42175..43269)
                     /gene="mnmH"
                     /locus_tag="ACHUPG_000453"
     CDS             complement(42175..43269)
                     /gene="mnmH"
                     /locus_tag="ACHUPG_000453"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR0860208.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(43338..44264)
                     /gene="allS"
                     /locus_tag="ACHUPG_000454"
     CDS             complement(43338..44264)
                     /gene="allS"
                     /locus_tag="ACHUPG_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFR0860209.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            44494..44976
                     /gene="allA"
                     /locus_tag="ACHUPG_000455"
     CDS             44494..44976
                     /gene="allA"
                     /locus_tag="ACHUPG_000455"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFR0860210.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            45054..45869
                     /gene="allR"
                     /locus_tag="ACHUPG_000456"
     CDS             45054..45869
                     /gene="allR"
                     /locus_tag="ACHUPG_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFR0860211.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            45959..47740
                     /gene="gcl"
                     /locus_tag="ACHUPG_000457"
     CDS             45959..47740
                     /gene="gcl"
                     /locus_tag="ACHUPG_000457"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFR0860212.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            47753..>48025
                     /locus_tag="ACHUPG_000458"
     CDS             47753..>48025
                     /locus_tag="ACHUPG_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFR0860213.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        11424 a        12502 c        12786 g        11313 t
ORIGIN      
        1 gtatgagatc ctgagttggt ggttcaatta ctccttaatg ttcgtaggtt atgcataaaa
       61 aggccgctta cgcggcctta gtgattacat gttgtatcaa tgatgatcga cagtatggct
      121 gtgctcgata tcttcattct tgcggctaaa gcggcggcga accaccacaa agaataccgg
      181 aacgaagaag attgccagta ccgttgcggt caccatcccg cccattacac cggtacctac
      241 tgcgttctgc gcgccggaac cagcaccagt actgataacc agcggcataa cgccgaggat
      301 aaacgccagc gaggtcatca ggatcggacg taaacgcatc cgcaccgcat caagcgtcgc
      361 ttcaatcaga cctttacctt ctttatccat caagtctttg gcgaattcga cgataagtat
      421 cgcgttcttc gccgacaacc caatggttgt gagcagtcct acttggaagt aaacgtcatt
      481 ggtcaggcca cggaaggtgg cagccagcaa cgcaccgata acccccagcg gaacgaccag
      541 cataacggag aacggagtcg accagctctc gtacagcgcc gccagacaca ggaatacgac
      601 aatcaacgaa atcgcgtaca gtgaaggtgc ctggttgccg gagagacgtt cctgatagga
      661 catccccgtc cagtcatagc caacaccggt aggcagtttg ctcgccagtt gttccatcag
      721 ctccattgct tcaccggtac ttttacccgg tgccgcctgg cctaagattt ccatggatgg
      781 caggccgttg taacgttcca gacgcggcga accgtactcc caacgagaag aggagaacgc
      841 cgagaatggc accatctgac catcagcagc acgaacatac cagtcgccga tatcatccgg
      901 cagcatacgg tatttcgctt ctgacatgac gtaaactttc ttcacacgac cgcggtcgat
      961 aaagtcgttc acatagctac cgccccatgc agcgcccaga gtggtgttaa tgtcgttgat
     1021 agaaacaccc agcgcctgcg ctttttcctg gtcgatatca atcttaaact gcggggtatc
     1081 ttccagaccg tttggacgta cgctggtcag catatcaggg tgcttcgctg cttctgcaag
     1141 cagctggtta cgcgcctgag tcagtttttc gtgaccaagg ccagcctggt caatcagctc
     1201 aaagtcaaag ccggttgcag tacccagttc cacgattgcg ggcaggttaa aggcgaaaac
     1261 catcgcatct ttgatttgcg agaaagcgcg tgttgcacgc atggtaatcg cttcaacttt
     1321 gttttcttcg cccggacgat cggcccagtc cttcaaggaa acgaacgcaa taccggtatt
     1381 ctgaccacgt cccgcaaagc cgaagccgtt aacggcgaac accgactcaa cgttgttctt
     1441 ttctttggtc agatagtaat gcgttacctc attgagcact ttctgcgtac gttcctgcgt
     1501 tgcacctgct ggcagctgaa ccatggtcat aaatacgccc tggtcctcat ctggcaagaa
     1561 ggagcttggc agacgcacga acagataggc catgccgacc acgatgatca gatacagcac
     1621 taggtaacgc cccgtactgc gcagaatacc gcctacgctg tcggtgtagt ggtgcgtgct
     1681 cttctcgaac atgcggttca accagccgaa gaagcctttt ttaccttccc cgtgatcgcc
     1741 tttggcaatc ggtttcagca tggtggcaca aagagccgga gtcaggatca acgctaccag
     1801 taccgacagc gccattgctg aaacaatggt aatagagaac tgacgataga tagcaccagt
     1861 agaaccgcca aagaaggcca tcggtacgaa taccgccgac agtaccatcg cgataccgac
     1921 cagagcgccc tgaatctgcc ccatcgactt acgggtagct tcttttggcg gcaaaccttc
     1981 ttccgccata acacgctcca cgttttctac cacaacgata gcgtcatcca ccaacaagcc
     2041 gatggcgagc accatcccga acattgttag cgtgtttatc gagaagccaa aggcggcaag
     2101 gacggcaaag gtcccgagca ataccaccgg tacggcaatg gtcggaatca acgtcgcgcg
     2161 gaagttctgc aggaacagat acataaccag gaacacgagg atgatcgctt cgaccagcgt
     2221 tttaaccact tcgtgaatag agattttcac gaacggcgtg gtgtcgtacg ggtaaacaat
     2281 tttcagaccc gacgggaaga acggttccat cttcgccagt tcagcacgga ttgccgcagc
     2341 ggtatccagc gcgtttgcac cggtcgccag cttgatcccc agaccggaag caggttggcc
     2401 gttaaactct gcgatgatgt cgtagttctc accacccagc tcaatcttcg ccacatcacg
     2461 cagcagtacg cgggaaccat cctgattcac tttcagcagg attttgccga actcttcagt
     2521 agaggtcaga cgcgtctgag caataataga ggcgttaagc tgttggcctt tcaccggcgg
     2581 cgtaccaccg agctgacccg ccgcaacctg ggcgttctgc gctttgatgg cggtaatgac
     2641 atcaaccggc gttagctgga atttgttcag ctcattcggg ttcatccaga tacgcatcgc
     2701 gtactgtgaa ccgaacaact gaacatcacc cacgcccgac gtacggctga tggcatcttt
     2761 catattcgcc gctacgtagt cagagatatc ctcctgcgtc atggtgccat cggtgttgat
     2821 aacgccgaca accatcagga agctgctgga tgatttctca acgctcaccc cttgctgctg
     2881 aacttcttgc ggcagcaacg gcatcgccag ctgcagtttg ttctgcacct gaacctgcgc
     2941 gatatccgca tcagtaccag actcaaaggt cagggtgatc tgcacggtac ccgtggagtc
     3001 actgttagag gacatgtaca tcaggttatc gataccgttc atattctgtt cgataacctg
     3061 tgtcaccgtg tcctgcactg ttttcgcatc agcgccgggg taggaggcgg agatcgttac
     3121 tgccggcggt gcaatcgtag gatattgcgc caccggcagt ttgaggatcg ccagcccccc
     3181 tgccaacatg atgataatgg cgatcaccca cgcaaaaatc gggcgatcga taaagaaatt
     3241 aggcatgtct taacggctcc tgtttaagtt aagacttgga ctgttcaggc tgagcaccgc
     3301 ttgcggcttg ctggttatta tcagcggtaa cttcttgtgc ttttacctgg acaccaggac
     3361 gcactttctg cagcccactt attactacgc gatcgcctgc tttcagacct tctgtcacca
     3421 gccacttatc gccaatagcc tggcttgcaa cgatcggacg ggtttccact ttgtcatccg
     3481 cgccaactac cagtacggtg gcatcaccac gcggcgtacg ggttacgccc tgttgcggga
     3541 ctaaaatagc gtttggatta agcccttctt ccagacgtgc gcgcacgaac atacccggca
     3601 gcagagtgtg atccgggttc gggaagatag cgcgtagggt gatagaccca gtggtctgat
     3661 caacggtaac gtcagagaat tccagcgtac cgtcctgcgg gaacttaatg ccgtcactgg
     3721 tgatcagcga cactttggct ttgccgttct cttgtttcag cgtgccattc gccagttcct
     3781 gtttcaggcg aaggaagtcg ttgctggact gggtcacatc aacgtagatc ggatcaagtt
     3841 gctgcacggt tgccagcaca gtcgcctgac cgttctgtac caatgcgcct tccgtcacgt
     3901 tcgacttacc aatgcgaccg ctaatcggag aggtgacttt ggtgtaagcc agattgatcc
     3961 gcgcagtttc aacggcagct ttcgccgcag ttaccgcagc attcgcctgt tgcgcatcag
     4021 ccagagcctg atcgtaccct tgcttactga tgtactgagt accgagcagt ttctgataac
     4081 gattcaccgt caattgcgcg atattggctg cagcctgggc tttcgccaga tcacctttcg
     4141 cactgtcgta tgtcgcctga taggtcgcag gatcaatctg atagagagag acaccagctt
     4201 cgatgtcgct accttctttg aaattacgct tcaggataat cccgctaact tgaggacgaa
     4261 cttctgcgat ccggtaggca ctggtgcgac ctggaagctc ggttgtgatc tgcagaggtt
     4321 cagttttgac tgttactacg ccaacggcgg gcatctgctg gccaccttgt tgggcctgtt
     4381 tgtcgtcaca tcctgttagg gctaagctgc ctgagagcat cagaacgacc gccagaggcg
     4441 taaaccctct gtttttgttc atatgtaaac ctcgagtgtc cgatttcaaa ttggtcaatg
     4501 gtcaaaagtt aataaaccca ttgctgcgtt tatattatcg tcgtgctatg gtacatacat
     4561 tcacaaatgt atgtaaatct aacgcctgta aattcacgaa catatggcac gaaaaaccaa
     4621 acaagaagcg caagaaacgc gccaacacat cctcgatgtg gctctacgtc ttttctcaca
     4681 gcagggggta tcatccacct cgctgggcga gattgcaaaa gcagctggcg ttacgcgcgg
     4741 tgcaatctac tggcatttta aagacaagtc ggatttgttc agtgagatct gggaactgtc
     4801 agaatccaat attggtgaac tagagcttga gtatcaggca aaattccctg gcgatccact
     4861 ctcagtatta agagagatat taattcatgt tcttgaatcc acggtgacag aagaacgacg
     4921 tcgattattg atggagatta tattccacaa atgcgaattt gtcggagaaa tggctgttgt
     4981 gcaacaggca caacgtaatc tctgtctgga aagttatgac cgtatagaac aaacgttaaa
     5041 acattgtatt gaagcgaaaa tgttgcctgc ggatttaatg acgcgtcggg cagcaattat
     5101 tatgcgcggc tatatttccg gcctgatgga aaactggctc tttgccccgc aatcttttga
     5161 tcttaaaaaa gaagcccgcg attacgtcgc catcttactg gagatgtatc tcctgtgccc
     5221 cacgcttcgt aatcctgcca ctaacgaata accctgaatc tgactccagg atttttcctg
     5281 gacattttcg tcgttgctat tctggttcac tgcgtcgtga tattcttgcg gtttgacttt
     5341 ttcaggtcgt tcttcaggtt cagaaacctt cattcatcat gactatgttc cagtattaca
     5401 aacgatcacg gcattttgtt ttttcagcat ttattgcttt tgtttttgtc ttgttatgcc
     5461 agaacacggc gtttgcgcgg gcgtcatcga atggtgatct gccgacaaaa gcggacctgc
     5521 aggcgcaact tgactcacta aataaacaaa aagatctttc tgctcaggac aaactggtgc
     5581 agcaggatct gacagataca ttagccaccc tcgataaaat cgatcgcata aaagaagaga
     5641 cagttcagct acggcaaaaa gtcgctgaag cgccggaaaa aatgcgccag gcgaccgcgg
     5701 cgttaacagc acttagcgat gtcgataacg acgaagaaac gcgcaaaatt ctgagtacgc
     5761 tttcgttgcg ccagctggaa actcgcgttg cccaggcgct ggacgatttg caaaacgcac
     5821 aaaacgatct ggcgtcttat aacagccagc tggtttcgtt acagacgcag cccgaacgcg
     5881 tgcaaaatgc gatgtataac gcttcgcagc agctgcaaca aattcgcagt cgtctggatg
     5941 ggactgatgt cggcgagact gccttacgtc ccagccagaa agtgttaatg caggctcagc
     6001 aggcgttgct gaatgcggag attgaccagc agcgtaaaag cctggaaggg aacaccgtct
     6061 tgcaggatac cttgcaaaag caacgtgatt acgtgacggc gaacagcgcc cgtctggagc
     6121 accagttgca gctgttgcaa gaagcggtaa acagcaagcg cctgacttta actgaaaaaa
     6181 cggcgcagga agccgtctcc ccggatgaag ccgcgcgtat tcaggctaat ccgctggtga
     6241 aacaggaact ggaaattaac cagcagttaa gtcagcgtct gattaccgcg actgaaaacg
     6301 gtaatcagtt gatgcagcaa aacattaaag tcaaaaactg gctggagcgg gcgctgcaat
     6361 cggaacgaaa tattaaagag cagattgccg tcctgaaggg cagcctgctg ttgtctcgta
     6421 tcctttacca gcaacaacaa acgctgccct cggcggatga actggaaaac atgaccaacc
     6481 gcatcgcgga tttgcgtctc gaacagtttg aagttaacca gcagcgtgat gcactcttcc
     6541 agagcgatgc gttcgtcaac aaactggaag aaggtcacac caacgaagtc aacagcgaag
     6601 ttcacgatgc gttattgcaa gtggttgata tgcgtcgcga attgctggat caactcaaca
     6661 aacagttggg taaccagctg atgatggcca ttaacctgca aatcaaccag cagcagttaa
     6721 tgagcgtatc gaaaaacctg aaatccatcc tgactcagca aatcttttgg gtgaacagta
     6781 accgtccaat ggactgggac tggatcaaag cgttcccgca aagcctgaaa gatgaattta
     6841 agtcgatgaa aatcacggtg aactgggaaa aagcctggcc tgccgttttt atcgctttcc
     6901 tcgccggttt gccgctgctg ttgattgccg ggctgatcca ctggcgtctg ggctggctga
     6961 aagcgtatca acagaaactg gcttccgctg tgggttccct gcgtaacgat agccagctca
     7021 acacaccaaa agcgatcctt atcgacctga tccgcgcgct gccggtgtgc ctgattattc
     7081 tcgcggttgg cctgattctg ttgaccatgc agctcaacat cagcgaactg ctatggtcgt
     7141 tcagtaaaaa actggcgatt ttctggctgg tgtttggcct gtgctggaag gtactggaga
     7201 aaaatggcgt tgccgtgcgt cacttcggca tgccggagca gcagaccagc cactggcgtc
     7261 ggcaaattgt ccgcatcagt ctcgcgttgc tgcctatcca tttctggtct gtggtggcag
     7321 aactttcccc gctgcatctg atggatgatg tgctggggca ggcgatgatt ttcttcaacc
     7381 tgctgctgat tgccttcctg gtatggccaa tgtgccgcga aagctggcgt gataaagagt
     7441 cgcacaccat gcgactagtc accattaccg tgctgtcgat tatcccgatt gcattgatgg
     7501 tgctgactgc cacgggttat ttctacacca cgctgcgcct ctccgggcgt tggattgaaa
     7561 ccgtttatct ggtgatcatc tggaacttgc tctaccagac ggtattgcgc ggcttaagcg
     7621 tagcggcgcg gcgtatcgcc tggcgtcgtg cgctggcgcg tcggcagaat ctggtgaaag
     7681 agggcgcaga aggtgccgaa ccgccggaag aacccaccat tgcactggag caagttaacc
     7741 agcagacgct gcgtattacc atgttgctga tgtttgcgct gttcggtgtc atgttctggg
     7801 caatttggtc cgatttgatc accgtgttca gctatctcga cagcatcacg ctctggcatt
     7861 acaacggcac tgaagctggc gctgcggtgg tgaaaaacgt caccatgggc agtctgttgt
     7921 ttgcgattat cgcctcaatg gtggcctggg cgttgattcg caacctgcct ggtttactgg
     7981 aagtgctggt gctctcgcga ctgaatatgc gccagggcgc gtcgtatgcc attactacca
     8041 tccttaacta catcattatt gccgttggtg cgatgacggt gttcggatcg ctgggcgtct
     8101 cttgggataa actccagtgg ctggccgcag cattatccgt aggtcttggt tttggtttac
     8161 aagaaatttt cggtaacttc gtctccggtt tgatcattct gttcgaacgt ccggtgcgta
     8221 ttggcgatac ggtaaccatt ggtagcttct cggggacggt aagtaagatc cgtattcgtg
     8281 cgacaacgat taccgatttc gatcgcaaag aagtgatcat cccgaacaaa gcgtttgtta
     8341 ccgagcgtct gatcaactgg tcgttgactg acactactac gcgtctggtg atccgtctcg
     8401 gcgtggccta tggctccgat ctggaaaaag tgcgtaaagt gttactgaag gcggcgactg
     8461 agcacccaag ggtgatgcac gaaccaatgc cggaagtctt ctttacggca tttggtgcca
     8521 gcacgttgga tcatgagctg cgtctgtatg tgcgtgaact gcgtgaccgt agccgtactg
     8581 tcgatgagct gaaccgtact atcgatcagc tgtgccgtga aaacgacatc aacattgcct
     8641 ttaaccagct tgaagtgcat ctgcacaacg agaagggcga tgaggtgacg gaagtaaaac
     8701 gcgactacaa aggcgacgat ccgacaccag cggttgggta ataaccaaag ggggcgacat
     8761 ttagttgccc cgagattact gacaaagtgc gcgttgttca tgccggatgc ggcgtgaccg
     8821 ccttatccgg cctacgaaac cgcaagaatt caatatattg caggagcggt gtaggcctga
     8881 taagcgtagc gcatcaggca gttttgcgtt tgcccgcaat cttaggggac atttagcggc
     8941 cccatttatt tctcactttt ccgcctcatc atcgtgcgtt aatttctttt cataatcacg
     9001 ctttacaata tccagcgcgc gcagcacggt actggcaggg atctgatttt cctccagcag
     9061 cacaatcaaa tcgacagcca gtttgacatc gtcaggggca ttttcaagtg acatactctt
     9121 tccattgcta gcgggttaag cgcgctagcc tgttttcgat tttttccagc gcatggcggc
     9181 agcgtgccag gcgcgcttca taggcttcca cttcacgatg cagcgtttgc tgttccacga
     9241 gatcggtcac ccgcgccagg cgggctctgc gttcggcaac catctcacgt agccgccgct
     9301 caaaatcctg atgctgaata cgtttacgct gccagcggga aattttcggt ggcgcactgt
     9361 cccactcgcg caatgaccag gcggaggctt cacgcgcgat ggcttccagt tgtgccgcca
     9421 gatgttccgc cagccaggcc acttgcggca gctgttgctg ctcaactgca tgacgaagcg
     9481 cagccagatt atcgcccgcc tcgtcgagac aagactgtag tgttgtcgca cgagtctgaa
     9541 aaagatgcct gtcgaaacga gcacttagcg tggcgaactg tgacaccggg gcacaacgct
     9601 gacgcagcgt agcgagctgt ccttccagtt tttccagcag cagggcggtt ttcacgataa
     9661 gacctcaatg aaaatgataa ttgttatgct aaagtagcca ctatgtaagc tgacaacact
     9721 caatatgcaa cgaatcattt taatcatcat tggctggctg gcggtagtgc tgggtacgct
     9781 gggcgtggta ttaccggtat taccgacgac gccgtttatc ctgctggcgg cctggtgctt
     9841 tgcccgttct tccccgcgct ttcacgcctg gttgctgtac cgctcatggt ttggcagcta
     9901 tctacgtttc tggcagaaac atcatgcgat gccgcgcggc gtcaaaccgc gggcgatttt
     9961 gcttattttg ctcacgtttg ccatttctct gtggttcgtc cagatgccat gggtgcgcat
    10021 catgttgctg gtaattctcg cctgtttgct tttctatatg tggcgaattc cggtgattga
    10081 tgaaaagcaa gaaaagcact gaagcacaac aatcgcagtt gcaattattg cgtacagcca
    10141 gtacattctg gcgttttcga gcacaggcgc aggcggtcaa aggttaaaca acagttactt
    10201 ttgatacgtt taaaacgcgc cgtgagtacc accgtaacaa gcaggcatac acttatgacc
    10261 gcgactgcac agcagcttga gtatctcaaa aatagcatca aaagcattca ggactaccca
    10321 aaacccggca ttcttttccg cgatgtcacc agcttactgg aagacccgaa agcttacgct
    10381 ctcagcatcg acttgctggt tgagcgttac aaaaatgcgg gcattaccaa agttgtcggc
    10441 accgaagcgc gtggcttctt gtttggcgct ccggtagctc tgggtctggg cgttggcttt
    10501 gtaccggtcc gtaaaccggg caaactgccg cgtgaaacca tcagtgaaaa ttacgacctg
    10561 gaatacggca ccgatcagct ggaaatccac gttgatgcca tcaaaccggg cgacaaagtt
    10621 ctggtggtgg acgatctgct ggcaaccggc ggcactatcg aagcgaccgt taaactgatc
    10681 cgtcgtctgg gtggtgaagt ggctgacgct gcgttcatta tcaacctgtt tgatctcggc
    10741 ggcgaacagc gtctcgaaaa acagggcatt accagctaca gccttgtccc gttcccgggc
    10801 cattaattat cgccagtctt gtgctgccca cgctacggac agcacaagat gtgcattcag
    10861 cctcgccgtt ctgacggggc tgtgttagca ttaccccttc gtgaatccac cttccagcgt
    10921 ttcagagcct gccaatgagt tatcaggttt tagcccgaaa atggcgccca caaacctttg
    10981 ctgacgtcgt cggccaggaa catgtgctga ccgcactggc gaacggcttg tcgttagggc
    11041 gtattcatca tgcttatctt ttttccggca cccgtggcgt cggaaaaacc tctatcgccc
    11101 gactgctggc gaaggggcta aactgcgaaa ccggcattac cgcgacgccg tgcggcgtgt
    11161 gcgataactg tcgtgaaatc gagcaggggc gctttgtcga tctgattgaa atcgacgccg
    11221 cctcgcgcac caaagttgaa gatacccgcg acctgctgga taacgtccag tacgctccgg
    11281 cgcgtggtcg tttcaaagtt tatctgatcg acgaagtgca tatgctgtcg cgccacagct
    11341 ttaacgcact gttaaaaacc cttgaagagc cgccggagca cgttaagttt ctgctggcga
    11401 cgaccgatcc acagaaattg ccggtgacga ttttgtcacg ctgtctgcaa tttcatctca
    11461 aggcgctgga tgtcgagcaa attcgccatc agcttgagca catcctcaat gaagaacata
    11521 tcgctcacga gccgcgggcg ctgcaattgt tggcgcgcgc cgctgaaggc agcctgcgag
    11581 atgccttaag tctgaccgac caggcgattg ccagcggtga cggccaggtt tcaacccagg
    11641 cggtcagtgc gatgctgggt acgcttgacg acgatcaggc gctgtcgctg gttgaagcga
    11701 tggtcgaggg caacggcgag cgcgtaatgg cactgattaa tgaagccgct gcccgtggta
    11761 tcgagtggga agcgttgctg gtggaaatgc tcggcttgtt gcatcgtatt gcgatggtac
    11821 aactttcgcc tgctgcactt ggcaacgaca tggccgccat cgagctgcgg atgcgtgaac
    11881 tggcgcgcac cataccgccg acggatattc agctttacta tcagacgctg ttgattggtc
    11941 gcaaagaatt accgtatgcg ccggaccgcc gcatgggcgt tgagatgacg ctgctgcgcg
    12001 cgctggcgtt ccatccgcgt atgccgctgc ctgagccaga agtgccacgc cagtcctttg
    12061 cacccgtcgc gccaacggca gtaatgacgc caaccctggt gccgccgcaa tcagcgccgc
    12121 agcaggcacc gactgtaccg ctcccggaaa ccaccagcca ggtgctggcg gcgcgccagc
    12181 agttgcagcg cgtgcaggga gcaaccaaag caaaaaagag tgaaccggca gccgctaccc
    12241 gcgcgcggcc ggtgaataac gctgcgctgg aaagactggc ttcggtcacc gatcgcgttc
    12301 aggcgcgtcc ggtgccatcg gcgctggaaa aagcgccagc caaaaaagaa gcgtatcgct
    12361 ggaaggcgac cactccggtg atgcagcaaa aagaagtggt cgccacgccg aaggcgctga
    12421 aaaaagcgct ggaacatgaa aaaacgccgg aactggcggt gaagctagcg gcagaagcca
    12481 ttgagcgcga cccgtgggcg gcacaggtga gccaactttc gctaccaaaa ctggtcgaac
    12541 aggtggcgtt aaatgcctgg aaagaggaga gcgacaacgc agtatgtctg catttgcgct
    12601 cctctcagcg gcatttgaac aaccgcggtg cacagcaaaa actggctgaa gcgttgagca
    12661 tgttaaaagg ttcaacggtt gaactgacta tcgttgaaga tgataatccc gcggtgcgta
    12721 cgccgctgga gtggcgtcag gcgatatacg aagaaaaact tgcgcaggcg cgcgagtcca
    12781 ttattgcgga taataatatt cagaccctgc gtcggttttt cgatgcggag ctggatgaag
    12841 aaagtatccg ccccatttga tcgtaagcac agcttacgtt cgtcatcctt aacgtgattg
    12901 agagagaaac ctatgtttgg taaaggcggt ctgggtaacc tgatgaagca agcccagcag
    12961 atgcaagaaa aaatgcagaa aatgcaggaa gagatcgcgc agctggaagt taccggcgaa
    13021 tctggcgcag gtctggtaaa agtgaccatc aacggtgcac acaactgccg tcgcgtagag
    13081 atcgacccga gcctgctgga agacgacaaa gagatgctgg aagacctggt ggctgcagca
    13141 ttcaacgacg cagcacgtcg tattgaagaa acgcagaaag aaaaaatggc ctctgtatcc
    13201 tccggaatgc agctgccgcc tggctttaag atgccgttct gatgcaaacc agcccgctgt
    13261 taacacagct tatggaagca ctgcgctgtc tgccgggcgt tggcccgaag tcggcgcagc
    13321 gtatggcgtt cacgctgctt cagcgcgatc gtagcggcgg gatgcgtctg gcgcaggcgc
    13381 tcacccgggc gatgtcggaa atcggccact gcgccgattg ccgcactttc accgaacagg
    13441 aagtctgtaa catctgttcg aatccgcgtc gtcaggaaaa cggtcaaatc tgtgtggtgg
    13501 agagtccggc ggacatctac gccattgagc agacggggca gttttctggt cgttattttg
    13561 tgttgatggg gcatctgtca ccgctggacg gcatcggtcc ggatgatatc gggcttgatc
    13621 gtctggaaca gcgtctggca gaggaaaaaa tcactgaagt gatcctcgcc accaatccca
    13681 cggttgaagg tgaagctacc gctaactaca ttgccgagct ttgcgcgcaa tatgacgtgg
    13741 aagccagccg aatcgctcat ggcgtaccgg ttggcggcga actggaaatg gtcgatggca
    13801 ccacgctgtc acactccctc gccgggcgtc ataagattcg tttttaagca aacgagagca
    13861 ggagcacctg ctctcgcttg aaattattct cccttgtccc catctctccc tcatcctgtt
    13921 tttaacctta aaatggcatt attgaggtag acctacatga aaggacaaga aactcgtggt
    13981 tttcagtcag aagtgaaaca gcttctgcac ctgatgatcc attctctcta ttccaataaa
    14041 gaaatcttcc tgcgtgagct tatctctaac gcctccgatg cggcggacaa gctgcgtttc
    14101 cgtgcgctat ctaacccgga cctgtacgaa ggtgatggcg aactgcgcgt tcgtgtctct
    14161 ttcgataaag acaagcgcac gctgaccatc tccgataacg gcgtggggat gacccgcgac
    14221 gaagtgattg accatctggg gactatcgct aaatccggta ccaaatcatt cctcgaatcc
    14281 ctgggttctg accaagcgaa agacagccag ctgatcggtc agtttggtgt tggtttctac
    14341 tctgcgttta tcgtggccga caaagtgacc gtgcgtactc gcgcggcagg cgaaaaacca
    14401 gaaaacggcg tcttctggga atcggctggc gaaggtgaat ataccgttgc cgatatcacc
    14461 aaagaagatc gtggtactga aatcaccctg catctgcgtg aaggcgaaga cgagttcctc
    14521 gatgactggc gcgtgcgttc catcatcagc aaatactccg accatatcgc gctgccggta
    14581 gagatcgaaa aacgcgaaga gaaagatggc gaaaccgtta tctcctgggc aaaaatcaac
    14641 aaagcgcagg cgctgtggac tcgtaacaag tcggaaatca ccgatgaaga gtataaagag
    14701 ttctacaaac acatcgccca cgactttaac gatccgctga cctggagcca caaccgcgtt
    14761 gaaggtaagc aggagtacac cagcctgctg tacatcccgt cccaggctcc gtgggatatg
    14821 tggaaccgtg atcataaaca cggcctgaaa ctgtacgttc aacgcgtgtt tatcatggac
    14881 gacgctgaac agttcatgcc gaactatctg cgcttcgtgc gtggtctgat tgactccagc
    14941 gatctgccgc tgaacgtttc ccgtgaaatc ctccaggaca gcacggtaac gcgtaacctg
    15001 cgcaatgcgc tgaccaagcg tgtgctgcaa atgctggaaa aactggcgaa agacgacgcg
    15061 gaaaaatacc agaccttctg gcaacagttt ggcctggtac tgaaagaagg tccggcggaa
    15121 gatttcgcta accaggaagc gatcgccaaa ctgctgcgtt ttgcttctac ttataccgat
    15181 tcttctgcgc agaccgtgtc tctggaagac tacgtttccc gcatgaaaga agggcaggag
    15241 aaaatctact acatcactgc agacagctat gcggcagcga agagcagccc gcacctggaa
    15301 ctgctgcgta agaaaggcat cgaagttctg ctgctttccg accgcatcga tgagtggatg
    15361 atgaactatc tgactgagtt cgacggtaaa ccgttccagt cggtgtctaa agttgacgag
    15421 tcgctggaaa aactggctga cgaagttgat gagagcgcga aagaagcgga gaaagcactg
    15481 actccgttca tcgaccgcgt gaaagctctg ctcggcgagc gcgtgaaaga tgtccgtctg
    15541 actcaccgtc tgaccgatac gccagcgatc gtttcgaccg acgcggacga aatgagcact
    15601 cagatggcga aactgttcgc tgcggcgggc cagaaagtgc cagaagtgaa atacatcttc
    15661 gaactgaacc cggatcacgt actggtgaaa cgtgcggcag atactgaaga tgaagccaag
    15721 ttcagcgagt gggtagaact gctgctggat caggcgctgc tggcagaacg cggcacgctg
    15781 gaagatccga acctgtttat tcgtcgtatg aatcagctgc tggtttcctg atgtcatgcc
    15841 ggatgacctt tgtgtcatcc ggcatttttc ttttcatcat ctgtactttc cgcaaattat
    15901 ctcgccatta accgtttcag cctcaggtgc ctttcttgag gcaatcgcct gttggtggta
    15961 tcgtttatcg ctttttcaaa aaaatcgaca cattttaagg ggattttcgc aatgcgtatc
    16021 attctgcttg gcgctccggg cgcggggaaa gggactcagg ctcagttcat catggagaaa
    16081 tatggtattc cgcaaatctc cactggcgat atgctgcgtg ctgcggtcaa atctggctcc
    16141 gagctgggta aacaagcaaa agacattatg gatgctggca aactggtcac cgacgaactg
    16201 gtgatcgcgc tggttaaaga gcgcattgct caggaagact gccgtaatgg tttcctgttg
    16261 gacggcttcc cgcgtaccat tccgcaggca gacgcgatga aagaagcggg catcaatgtt
    16321 gattacgttc tggaattcga cgtaccggac gaactgattg ttgatcgtat cgtaggccgc
    16381 cgcgttcatg cgccgtctgg tcgtgtttat cacgttaaat tcaatccgcc gaaagtagaa
    16441 ggcaaagacg acgttaccgg tgaagaactg actacccgta aagacgatca ggaagaaacc
    16501 gtgcgtaaac gtctggttga ataccatcag atgactgcac cgctgatcgg ctactactcc
    16561 aaagaagcgg aagcgggtaa caccaaatac gcgaaagttg acggtaccaa gcaggttgct
    16621 gaagttcgcg ctgatctgga aaaaatcctc ggctaatcaa gccgcagcag atctgctttc
    16681 gggcaggtct gcttatccac ctcaggcaat acctctcaca gcaattagtt cttcttcctc
    16741 acttttccgc tacaattatc aacaagttga atcgataaga ggcggtaatg cgtcagacta
    16801 aaaccggtat cctgctggca aacctgggta cgcccgatgc ccccacacct gaagcggtaa
    16861 aacgctatct gaaacaattt ttaagcgaca gacgcgtggt tgatacctca cggttgttat
    16921 ggtggccgtt gctgcgcggc gtgattttgc cgctgcgctc gccgcgtgtg gcgaagctgt
    16981 acgcctctgt ctggatggaa gacggctcgc cgctgatggt ttacagccgc cagcaacagc
    17041 aggcgttggc acaacgttta ccggatacgc cagtggcgct gggcatgagc tacggctcgc
    17101 catcactgga aagctccgtc gatgaactcc tggcagagca tgtagatcat attgtggtgc
    17161 tgccgcttta tccgcaattc tcctgttcta cggtcggtgc ggtatgggat gaactggcac
    17221 gcattctggc gcgcaaacgt agcattccgg ggatatcgtt tattcgtgat tacgccgata
    17281 accacgatta cattaatgca ctggcgaaca gcgtacgcgc ttcttttgcc aaacatggcg
    17341 aaccggatct gctgctgctc tcttatcatg gcattcccca gcgttatgca gatgaaggcg
    17401 atgattaccc gcaacgttgc cgcacaacga ctcgcgaact ggcttccgca ctggggatgg
    17461 taccggaaaa agtgatgatg acctttcagt cgcgctttgg ccgggaaccc tggctgatgc
    17521 cttataccga cgaaacgctg aaaatgctcg gagaaaaagg cgtaggtcat atacaggtga
    17581 tgtgcccggg ctttgctgcg gattgtctgg agacgctgga agagattgcc gagcaaaacc
    17641 gtgaggtctt cctcggtgcg ggcgggaaaa aatatgaata tattccagcg cttaatgcca
    17701 cgccggaaca tatcgaaatg atggctaatc ttgttgccgc gtatcgctaa agctgagcgg
    17761 taaagaactg agcgccgtcg cgaagagcct cgtcggcggt tttcatcatc cgtgaataat
    17821 gcaaaaaggc atgcagcgta cctgggtaga gtttgaactc acagggctgc tgatgcgccg
    17881 ctaacgtcta gtaaagcaga cggctgtcat ccagcagcgg atcgaactcc gccccggcaa
    17941 taaaacaggg cggaacttcg cgagtgagat cattattaaa cagacagtaa tacggtgact
    18001 cgcggtccgc gtcgttgctt aaatacgcct cttcatacat ctgcaaatcc tgttgcgtta
    18061 agccatccca gacaccgccc aacagacgac gagtcacgga atcccgtaat ccgtaaagcc
    18121 cataccacag caaaacaccc gcaattttac cgcaatcgat ctgtttatca cgcaaccaca
    18181 acgcactggc gagcgccagc atggcacctg cggaatcacc ggcaaagcca atgcgggaca
    18241 tattgatttg ataatcctcc gcctgctggt ggaaataaca acaagcagcc acaatttcct
    18301 ctatcgcttg tggaaaacgc gcttcaggtg aaagggtgta attaatacca atcaccgtac
    18361 attggctgta gcttgccagc aggcgcatga tgcgatcgtg ggtatcgaga ttgccgagaa
    18421 taaaaccgcc tccatgcaaa taaaatagcg tcgccgggct atctggctgc ggacaaaaga
    18481 gacgtgtttc cacctgccca tattttgttg gaaccatgta agctctggtt gccatttctg
    18541 gagcgcccgc attccagaat cggcgctcaa gcgtgtaata ctgtcgttgc tcagcaatcg
    18601 ttcccgttgc gggccaggac ggtaaatccg gctgaagagt attcacaacg gtcttcattt
    18661 cagcagaaat aaggtccaga acaggtagtt tgttttccgg cttcatacaa aaactccttt
    18721 caaattacgt cattgtaagg aaccactgcc atgaaaatgc gatcccgcct gctgatattg
    18781 aaactggctg cgtctcgcgc gctcccgtca gattgtgtta acattcgccg ctcagttaac
    18841 cacccgtaaa aacaaccatg aaatttcccg gtaaacgtaa atccaaacat tacttccctg
    18901 taaatgcacg cgatccgctg cttcagcaat tccagccaga aaacgaaacc agcgccgcct
    18961 gggtcgtagg tattgaccag acgctggtcg atattgaagc gaaagtggat gatgaattca
    19021 ttgagcgtta tggattaagc gccgggcatt cactggtgat tgaggatgac gtagccgaag
    19081 tgctttatca ggagctaaaa cagaaaaacc tgattaccca tcagtttgcg ggtggcacca
    19141 ttggtaacac catgcacaac tactcggtgc tcgcggacga ccgttcggtg ctgctgggcg
    19201 tcatgtgcag caatattgaa attggcagtt atgcctatcg ttacctgtgt aacacttcca
    19261 gccgtaccga tcttaactat ctacaaggcg tggatggtcc gattggtcgt tgctttacgc
    19321 tgattggcga gtccggggaa cgtacctttg ctatcagtcc aggccacatg aaccagctgc
    19381 gggctgaaag cattccggaa gatgtgattg ccggagcctc ggcactggtt ctcacctcat
    19441 atctggtgcg ttgcaagccg ggtgaaccca tgccggaagc aaccatgaaa gccattgagt
    19501 acgcgaagaa atataacgta ccggtggtgc tgacgctggg caccaagttt gtcattgccg
    19561 agaatccgca gtggtggcag caattcctca aagaccacgt ttctatcctt gcgatgaacg
    19621 aagatgaagc cgaagcgttg accggagaaa gcgatccgtt gttggcatct gacaaggcgc
    19681 tggactgggt agagctggtg ctgtgcaccg ccgggccaat cggcttgtat atggcgggct
    19741 ttaccgaaga cgaagcgaaa cgtaaaaccc agcatccgct gctgccgggc gctatagcgg
    19801 aattcaacca gtatgagttt agccgcgcca tgcgccacaa ggattgccag aatccgctgc
    19861 gtgtctattc gcacattgcg ccgtacatgg gcgggccgga aaaaatcatg aacactaatg
    19921 gagcggggga tggcgcattg gcagcgttgc tgcatgacat taccgccaac agctaccatc
    19981 gtagcaacgt accaaactcc agcaaacata aattcacctg gttaacttat tcatcgttag
    20041 cgcaggtgtg taaatatgct aaccgtgtga gctatcaggt actgaaccag cattcacctc
    20101 gtttaacgcg cggcttgccg gagcgtgaag acagccagga agagtcttac tgggatcgtt
    20161 aagttatcgt cggttcgtag gccagataag gcgttcacgc cgcatctggc atttggctct
    20221 cgatgcctga tgcgacgctg gcgcgtctta tcatgcctac atatttttca tattttacat
    20281 ccggcaacca ccgtttaccc cgtcaccact tcacccgccg gtggcgtttc cagcagttcc
    20341 agcatagtac gggcaatttc acgttcaccc atcactacct gattcgcgcc acgttcggtg
    20401 atatacgtca cttcatcgtc ataatgggcg cgggcgataa tctcaatatc cgggtttttc
    20461 gcgcgggcag acgccacaat ctcacccgct tcataaccgt tggggatcgt caggatcagc
    20521 cattttgcac attccagatg tgccagttgc ataatttctt cgttcgctgc attgcccaat
    20581 acagcgcgga ccccacgctc tcgcagctca tcaacacggg ttcgtgacgt ctcaatcacc
    20641 accagcggaa tatcagaggc gagcaatttc tcccccagca ggctgcctac acgaccgtaa
    20701 cccaccaata gcgcatggtt gcaaatatcc actgggatct gtttctcttc ttcgattgcc
    20761 tcttccagcg tctgctcttc cagcgtttcg gtcttcgcca gatatttctc cagtagtgcg
    20821 aacagtaccg ggttgagcat aatcgacagg atcgcccctg ccagtaccag gttttgtccg
    20881 gcctgcggca gtaaattcaa tgccattccc agtcccgcca ggataaacgc gaactcacca
    20941 atctgcgcca ggctggcggc gatggttaat gccgtacgtt gggagtgacc aaacagacgg
    21001 acaaggaaca gggccgcgac cgatttacca aacagaataa ttgccagcgt cgccagcact
    21061 gccagcggtt gctgaatcag aattaacgga tcaaacaaca tcccgactga gacaaaaaac
    21121 agcaccgcaa acgcgtcgcg caatggcaac gtatcgtggg cggcacggtg actcaattca
    21181 gactcgttca gtaccatccc ggcaaagaac gcaccgagtg caaaggagac atcaaacagc
    21241 tctaccgcac caaaggcaat ccctaacgcc agcgccagca ctgacagggt aaacagctcg
    21301 cgagaaccgg ttgccgcgct gcgtgccata atccacggca ccagacggcg acctaccagc
    21361 atcataatgg cgataaatgc gatcaccttg ccgatggtga tccccatatc gactgcaaga
    21421 gtggcaaagc ccacatcgcc ctgttccatc attcctgcca ctgcgggcag caacaccagc
    21481 gtcagaacca tcaccaggtc ttccacaatc aaccaaccga tggcgatttg cccacgctga
    21541 ctgtctatta attgccgttc ttcaagtgcg cgcagtaaca ccacggtact ggcggtggaa
    21601 agacataagc cgaacacgat accggtcatt aacgaccagc ccagcacggc ggagagcgcc
    21661 atacccagca gcgtcgccac ggctatctgg gcgatcgcgc cgggaatggc gatggacttt
    21721 accgccatca aatccttgag cgaaaaatgc aaaccgacgc cgaacatcag caaaataacg
    21781 cccagttcag ccagttccgg cgcaagcttg gtatcggcaa caaagcccgg agtgaatggt
    21841 cctgccagca cacccgctaa cagatatccc accagaggag aaatacgtag tttattggcc
    21901 agcatgccga ggataaaggc gagcacaagg ccgccaacaa tggtggtgat aagcggggtg
    21961 gcgtgatgca ttccgtctcc ttttcctggt ggttattgtc catttttggc tgggaaaacc
    22021 aaaattacag gtaatagttt atgacaattt cattgatgat gtttatgaat aattgttgaa
    22081 ttttgcagaa aaatggaatt agctgcaaaa aaagcacggt tcggaaaaca gaaggcggtt
    22141 taacagagga aaggcttatg gcgactgtaa aacgatgcag ccaaagtttg cctttggctg
    22201 caatgaaatc agtctttatg ccggttatca ggcaggaata tggtcaacat ccccaatagt
    22261 ggcaggaaag cacagatttt atagactaac tcgatgctgg tgtgatcggc gataagcccc
    22321 agaactgccg ctcccagacc tcccatgccg aaagcaaaac cgaaaaagag tccagaaacc
    22381 ataccgatac gtcccggaag tagctcctga gcgtagacca gaatggcaga gaatgccgaa
    22441 gcgaggataa atccaataat caccgttaaa acccccgtcc agtgcaggcg ggcgtagggt
    22501 aaaatcagcg taaacggcgc aacgccgagg atagagcccc aaatcacata tttccgtcca
    22561 attttatccc ctacaggccc gccgatcacc gtacctgccg caacggcaaa caggaaggca
    22621 aacagatgaa gctgggcatt ctggatagat aatccgaatt tttgcatcag ataaaaggtg
    22681 taatagctgc tgatgctcgc catatagaaa tatttcgaga aaatgaggat taacagaatg
    22741 ctgactgcca gtaccacttt gttgcgcggc agtggattga ttatcgtcgc tttgggtttt
    22801 cctttattca ttcggtgctg tgccgagtac caacggctaa tttgcgccaa caccacgatc
    22861 gccagcagtg ccgcaagcac aaaccaggca acgttgcctt tgccataagg ctcgataatc
    22921 accgccgcca gcaagggtcc cagggaactg ccaaagttgc cgccgacctg aaagatagat
    22981 tgcgccaggc catgccgccc gccggaagcc atacgggcta cgcgagaaga ttccggatga
    23041 aagaccgatg aaccggtacc gaccagcgcc gctgccagca gaactgcgcc aaaactgccc
    23101 gccagcgcaa gcagcaccag accacttaag gtaaagcaca tgccaattgg caacgaccac
    23161 ggcatcggat atttatcggt ccagtagccg accactggtt gcagtagcga agaggcgagc
    23221 tggaaggtga gggttatcat gccaatctgc ataaatgtca gagaaaattc tgactgaagc
    23281 agcggataaa tcgccagaat cagcgattgg atcatgtcgt tcagcagatg tgagaggctg
    23341 atagcaccta aaataccaaa cgatgttcgg gccttggtcg ttgacgcagc cgcgcccgcc
    23401 acaggctggg tttgttcact cattgccata ggaaagtcac tttttcaggg ttgcgatgta
    23461 aagaatgatc ttatttgtga ttattacccg actaacatac ctgtatgcgt cgtctgaagg
    23521 aagtctcaac gccgaataca gaatttctaa tctggatgca gatttatctt caccggacgc
    23581 agacttgtct atgatgttgc gtcatactat ttttcaacac gttgaaatca ggtcagggag
    23641 agaagtatga aattattgca gcggggcgtg gcgttagcgc tgttaaccac atttacactg
    23701 gcgagtgaaa ctgctctggc gtatgagcag gataaaacct acaaaattac agttctgcat
    23761 accaatgatc atcatgggca tttttggcgc aatgaatatg gcgaatatgg tctggcggcg
    23821 caaaaaacgc tggtggatgg tatccgcaaa gaggttgcgg ctgaaggcgg tagcgtgctg
    23881 ctactttccg gtggcgacat taacactggc gtgcccgagt ctgacttaca ggatgccgaa
    23941 cctgattttc gcggtatgaa tctggtgggc tatgacgcga tggcgatcgg taatcatgaa
    24001 tttgataatc cgctcaccgt attacgccag caggaaaagt gggccaagtt cccgttgctt
    24061 tccgcgaata tctaccagaa aagtactggc gagcgcctgt ttaaaccgtg ggcgctgttt
    24121 aagcgtcagg atctgaaaat tgccgttatt gggctgacaa ccgatgacac agcaaaaatt
    24181 ggtaacctgg aatacttcac tgatatcgaa tttcgtaagc ccgccgatga agcgaagctg
    24241 gtgattcagg agctgcaaca gacagaaaag ccagacatta ttatcgcggc gacccatatg
    24301 gggcattacg ataatggtga gcacggctct aacgcaccgg gcgatgtgga gatggcacgc
    24361 gcgctgcctg ccggatcgct ggcgatgatc gtcggtggtc actcgcaaga tccggtctgc
    24421 atggcggcag aaaacaaaaa acaggtcgat tacgtgccgg gtacgccatg caaaccagat
    24481 caacaaaacg gcatctggat tgtgcaggcg catgagtggg gcaaatacgt gggacgggct
    24541 gattttgagt ttcgtaatgg cgaaatgaaa atggttaact accagctgat tccggtgaac
    24601 ctgaagaaga aagtgacctg ggaagacggg aaaagcgagc gcgtgcttta cactcctgaa
    24661 atcgctgaaa accagcaaat gatctcgctg ttatcaccgt tccagaacaa aggcaaagcg
    24721 cagctggaag tgaaaatagg caaaaccaat ggtcgtctgg aaggcgatcg tgacaaagtg
    24781 cgttttgtac agaccaatat ggggcggttg attctggcag cccaaatgga tcgcactggt
    24841 gccgactttg cggtgatgag cggaggcgga attcgtgatt ctatcgaagc aggcgatatc
    24901 agctataaaa acgtgctgaa agtgcagcca ttcggcaatg tggtggtgta tgccgacatg
    24961 accggtaaag aggtgattga ttacctgacc gccgtcgcgc agatgaagcc agattcaggt
    25021 gcctacccgc aatttgccaa cgttagcttt gtggcgaaag acggcaaact gaacgacctt
    25081 aaaatcaaag gcgaaccggt cgatccggcg aaaacttacc gtatggcgac attaaacttc
    25141 aatgccaccg gcggtgatgg atatccgcgc cttgataaca aaccgggcta tgtgaatacc
    25201 ggctttattg atgccgaagt gctgaaagcg tatatccaga aaagctcgcc gctggatgtg
    25261 agtgtttatg aaccgaaagg tgaggtgagc tggcagtaat ccgaaagtgc cggatgtttg
    25321 catccggcac aatgcttaat cgcggcgggc gatatcagca aatttggcat cgaggatctt
    25381 tgccagatcg cctgccgcca gttcgatatc cagtccgcgc ttgccgccgg aaacataaat
    25441 agtggcaaat tcttgtgcgg gggcgtcgat aatcgttggc agacgttttt tctgccccag
    25501 tgggctaatc cccccaacca ggtatcccgt cgaacgctgc gcgaccatcg gatcggccat
    25561 ctcaactttc ttggcaccca gcgcttttgc tactttttta agatccagtt gacctgcgac
    25621 cggcgtaacg gccacggcaa ggtgtttcat atcaccgttc actgccacca gcagcgtttt
    25681 gtagacctga tccggattca aacctaattt tttgacgact tcatcgccaa aattggtttc
    25741 agccggatcg tgctcgtagg tatggatttg aaacgaaatc ttgttttttt cgagtaattt
    25801 aactgcgggt gtcatggtaa ttttcctgcc ttaaacaaaa aaacgaagca agcatacgcc
    25861 tgatggcggt ccaaaaaaaa gagtcatctt gcctaagagt attggcagga tggtgagatt
    25921 gagcgacaat cgagttacac cgtcgcttaa agtgacggca taataataaa aaaatgaaat
    25981 tcctctttga cgggccaata gcgatattgg ccattttttt agcgcaacat ttgcggcaaa
    26041 ttcccttctc catacaggtg tagtgcaccg accgcgacca catatcgccc cggcggcatg
    26101 gcgcgtaatt tatcccgcca ggcgagattt cgctgatgca tcagcacatc gtacagcgac
    26161 tgactgaacg tattgggcag cgttatatca ttattttgcg gcggtgcatt cagccaccag
    26221 ctcatcattt gttgcagcaa ccgtgcgttg gtatgccagt gggtcagcgt atcgtccagc
    26281 agcgccagtc ctttgtcagg gagctggagc aacatggcaa tctggttttc agccccttcc
    26341 agttcaatca cgggtttatg ttgttgcttc gccgcctgca atagctggta atcgataccg
    26401 tattctgccc gcagccccag tttttgtgcc tgcgtcgcct gaagaaccat cgcgatttgc
    26461 cacagcggtt gggtagaaaa gagtgaggga gaaatgccca tctcctggct aatgtgctgc
    26521 aggttttgga gttgctcctc gctaatgcgc tcttccagcg cctcgcaggc aggcaaatta
    26581 gcaaaaggcg tatcgctggt ggaaacatcc gcctcgacga tcagcgcatc ggcgtttttg
    26641 agctttttga gcaaacgggt gggcagggga gccatatcgt ggctacccat atgaatactg
    26701 ccaatcagat gaaaatggcg attgccgggg agggtgatat cgatggcagg ccaggtgtaa
    26761 tgattaccgc gcagcgcggc ccaaagcgtt tttacccggt acaacagatc catacgacct
    26821 ccctttgtga aatatcatgc tagcgcgcgg tgagggatgg cgcaaatgga gttggggact
    26881 tgtttaatgt gtttgtatga ttcagtatgt tcttgcatcg ctattcacag ggaagcaaca
    26941 gttaaaaacc atgaaacagg caacaagaaa accgacgaca cccggagata ttcttctcta
    27001 tgaatatctg gaaccgctcg atttgaaaat caatgagtta gcagagttgc tgcatgttca
    27061 tcgtaatcgc gtcagtgcac tgatcaataa caatcgtaaa ctcaccactg agatggcatt
    27121 ccgtctggcg aaagtttttg ataccacagt cgattttagg ctaaacctcc aggcggcagt
    27181 tgatctttgg gaagttgaaa acaacatgcg cacccaggaa gaattgggac ggattgaaac
    27241 agtggctgaa tatttggcac gccgtgaaga gcgtgcaaaa aaggtcgcgt aaggccaaaa
    27301 catcgcttca tctcgtaggc cggataaggc gttcacgccg catccggcat ccgagcacca
    27361 ttgcctgatg cgacgcttac gcgtcttatc aggcctacaa acctgtgcct gaaccgtagg
    27421 ccggataagg cgtttacgcc gcatccggca gtcatgcgtc gatgccaaat gcgccgccct
    27481 aaagcagcgc atccgcaatg acgtacttat tccttcggtt taaaccgcag caaccggttg
    27541 gcgttactta ctacggtaat tgacgagagc gccattgccg ctccggcaac taccgggtta
    27601 agcagtgttc cagtaaacgg ccacaaaata ccggcggcga ccggaatacc gatgctgttg
    27661 tagataaagg caccgagcag gttctgcttc atgttgcgca gcgttgcgcg ggaaatcgcg
    27721 agcgcatccg caacgcccat caggctatgg cgcatcaggg taatcgccgc ggtttcaatg
    27781 gcaacatcac tgccgccacc catcgcaatg ccgacatccg cctgggccag cgctggcgcg
    27841 tcgttaatgc cgtcgcccac cattgccacc tgacgtcctt cactttgcag acgtttgatc
    27901 gcttcggctt taccgtccgg cagcactccg gcaatcactt catcaattcc agcttctttg
    27961 gcgatcgcat tggcggtggt tgggttatcc ccggtcaaca tcaccagacg atatcccgct
    28021 ttatgcaggc gttgcagcgc cgccacgcta tcactacgca acggatcgcg tacagccagc
    28081 agggctactg ctttcccgtc aatcgccagc agcacaggcg ttgctccttg cgatgcctga
    28141 gctgaaatat cggcttcaat cgctttagta tcgacttgct gatcatttaa caacgcctga
    28201 ttacccagca ataacgcatg accttctgct tcaccgctca cgcccagccc gcgcaatgtg
    28261 cggaaaccgt tgacctgcgg tagctgcata tcgcctgctt tatcaaggat cgctcgtgcc
    28321 aatggatggc tggaaccttg ctccagcgcc gccgccagac gcagcgcctg cgcttcgtca
    28381 acatcagcaa atgttttcac tgcgacaacc tgcggcttcc cttcggtcag cgtcccggtt
    28441 ttatcgaaca cgacggtatc gagtgtactg gcgcgttgca gcgcgtcggc gtcccgcacc
    28501 agcacgccaa actcagccgc ccgcccgacg ccggaaataa tcgacatcgg cgtcgccagt
    28561 cccagcgcac acgggcaggc gataatcagc accgtagtgg caatcaccag ggtatagaca
    28621 atctgcggtg ccggaccaaa gaaataccag attgccgcac tgacaagcgc aataaccact
    28681 actaccggca caaatatggc tgagatttta tccgccagct gaccgatttc tggcttgctg
    28741 ctctgggcct ggcgcaccat gcgaatgatc cttgatagcg tggtatggct gccaaccgca
    28801 ctggcacgaa acagcacact gccgtcctgt accactgtcc cggcatggac gctatcgcct
    28861 tcgccttttt gctgcgggat tggttcgccc gtcagcatcg cttcatccag ccatgcttcg
    28921 ccctgggtaa tctcgccatc taccggcacg cgatcgccgg tcgtcaggcg cagcaacata
    28981 cctggctgca cttctgccag aggcacgctt ttttcacctt cgtcagtaac caggcgcgcc
    29041 gtcggtgggg ttaaatcgag taatttctcc agtgccttag aagaacgctg acgtgcgcgt
    29101 gcttccagca tatggccgag attgatcaga ccgataatca tcgcactggc ttcatagtaa
    29161 agatgtcgcg cttccatcgg gaaccactgc ggccacaggt tgacgctcat cgaatagagc
    29221 cacgccacgc ccgttcccag cgccaccagc gtatccatcg tcgctgcacc gttcagcagg
    29281 cttttccatg cactgcggta aaaatggccg ctggcgaaaa ccatcaccgc cagggttatc
    29341 aggccgataa ccaaccacag gctgcggttg tcggcggtga ctatcatgtt atcgccgatc
    29401 atcccccaga ccatcaccgg gatacccacc gccagtgcga caattgcctg ccagcggaag
    29461 cgcttcatcg tagcgacggc ggtttcttgc tggcgttcgc ggcgtttcgc gtcatcttca
    29521 atcgcttccg cgccgtagcc cgctttttcc accgcctgca ctaaatcttg tggggaggca
    29581 ctgcccatca ccagcgcagt acgctccgcc aggtttaccc gtgcctgagt gacgcccggt
    29641 acgctttgca gcgcattttg tacgcgggtg acacagctgg cgcagctcat gccgctcagc
    29701 agcaactgct ggctgtcatc gtcatcggcg gtcgctgccg gaagcgcctc agaaaccgct
    29761 gtcagtgctt ccgacgggat tgatgactcc gccagcggtt tagcctttgg gtggcttaca
    29821 gatgcgtcat aacccgcttg tttgatggtt tcgatcagct gttctgcact ggcagtcccg
    29881 gtaacgtgcg cttcagtgat agacacatcc gcttgttcca catccgggcg ctgttcaaga
    29941 ctttctttca cgcgtttaac gcagtgaccg caggacaggc cgtccaggtt caggtcgata
    30001 gtttgtgaca taaaacactc ctttaagaca gttttgactg gctgtgataa aggttaaacc
    30061 ttccagcaag gggaaggtca agaaattaat aaaccaggcg ggtaaaagtc cgtaaagatt
    30121 aaaaaatcgg ctcgatttgc atcaggatta gacatttatt tctttgtttt cctgtagtta
    30181 agttgcgggt gctaagttaa atcaggatgc ctgaaaatcg gcactggggt gaggaattac
    30241 ctcccgcatc tataaaaagg agttaacaaa agatgttaga tgcaaacaaa ttacagcagg
    30301 cagtggatca ggcttacacc caatttcact cacttaacgg cggacaaaat gccgattaca
    30361 ttccctttct ggcgaatgta ccaggtcaac tggcggcagt ggctatcgtg acctgcgatg
    30421 gcaacgtcta tagtgcgggt gacagtgatt accgctttgc actggaatcc atctctaaag
    30481 tctgtacgtt agcccttgcg ttagaagatg tcggcccgca ggcggtacag gacaaaattg
    30541 gcgctgaccc gaccggattg ccctttaact cagttatcgc cttagagttg catggcggca
    30601 aaccgctttc gccactggta aatgctggcg ctattgccac caccagcctg attaacgctg
    30661 aaaacgccga acaacgctgg cagcgaattt tacatatcca acagcaactg gctggtgagc
    30721 aggtagcgct ctctgacgaa gtcaaccagt cggaacaaac aaccaacttc cataatcggg
    30781 ccattgcctg gctgctgtac tccgccggat atctctattg tgatgcaatg gaagcctgtg
    30841 acgtgtacac ccgtcagtgc tctacgctca tcaatactgt tgaactggca acgcttggcg
    30901 cgacgctggc ggcgggtggt gtgaatccgt tgacgcataa acgcgttctt caggccgaca
    30961 acgtgccgta cattctggcc gaaatgatga tggaagggct gtatggtcgc tccggtgact
    31021 gggcgtatcg cgttggttta ccgggcaaaa gcggtgtagg tggcggtatt ctggcggtcg
    31081 tccctggcgt gatgggaatt gccgcgttct caccaccgct ggacgaagaa ggcaatagtg
    31141 ttcgcggtca aaaaatggtg gcatcggtcg ctaagcaact cggctataac gtgtttaagg
    31201 gctgatcatg atgaacacgg aaggtaataa cggtaacaaa cccctcggtc tatggaacgt
    31261 cgtttccatc ggtattgggg caatggtggg ggcggggatc ttcgcgctgc tggggcaggc
    31321 tgcgttgcta atggaagcct cgacctgggg cgcctttgct tttggcggta ttgtggctat
    31381 gttttccggt tatgcctatg cgcgtctggg ggcgagctat cccagcaatg gcggtattat
    31441 cgactttttt cgtcgcggat taggcaacgg cgtcttttcg ctagcgctct cgttgctata
    31501 cctgttgacg ctggcggtga gcatcgccat ggttgcccgt gcttttggcg cttatgccgt
    31561 gcagtttttg catgaaggca gccaggagga gcaccttatt ttgctctacg cgttggggat
    31621 cattgcggtg atgacgcttt tcaactcctt aagcaaccat gcggtagggc ggctggaagt
    31681 gatcctcgtc ggcattaaaa tgatgatcct gttattgctg attattgccg gtgtctggtc
    31741 gctgcaaccg gcgcatattt ccgtctctgc gccccccagc tccggtgcgt tcttctcctg
    31801 tattgggata accttccttg cctatgcggg ctttggcatg atggcgaacg cggcggataa
    31861 agtgaaagat ccgcaggtca ttatgccacg ggcgtttctg gtggcgattg gcgttaccac
    31921 gttgctttat atctcgctgg cgctggtttt gcttagcgat gtatcggcat tagagttaga
    31981 aaaatatgct gataccgccg tagcgcaggc tgcttctccg ctgctcgggc atgtgggtta
    32041 tgtgatcgtc gtcatcggcg ctttactggc gacggcttca ggcattaacg cgaacctgtt
    32101 cgccgtgttt aacatcatgg acaacatggg cagcgaacgc gaactgccga agctaatgaa
    32161 taaatccctg tggcggcaga gtacctgggg caatatcatc gtcgtggtgt tgataatgct
    32221 gatgacggcg gcgctaaatt tagactcact cgccagcgtt gccagcgcca cctttttgat
    32281 ctgctatctg gcggtgtttg tggtggcgat ccgcctgcgt catgatattc acgcctcgtt
    32341 gccgattctt atcgttggta cgttggtgat gttgttggtg atcgttggct ttatctacag
    32401 cctgtggtcc cagggcagcc gtgcgttgat atggattatt ggtgcaatct tactcagcct
    32461 tattgtggca atggtcatga agcgtaataa aaccgtataa cacctctctg tgcgcagtac
    32521 ttcctgtatt attgtggtgg cggtcgatat tcgcactggc aaaaaaacgt gcttgaatat
    32581 ctgttgaaac cctttaacaa agcacaggag gcgttgcgcg aacgatgaac atcagcgatg
    32641 tagcaaaaat taccggcctg accagcaaag ccattcgctt ctatgaagag aaggggctgg
    32701 tgacgccgcc gatgcgcagc gaaaacggtt atcgcaccta cacgcagcag catctcaacg
    32761 aactgacctt actgcgccag gcacggcagg tgggctttaa cctggaagag agcggcgagc
    32821 tggtgaatct gtttaacgac ccgcagcggc acagcgccga cgtcaaacgg cgcacgctgg
    32881 agaaggtggc ggagatcgaa cgacacattg aggagctgca atccatgcgc gaccagctgc
    32941 tggcactggc gaatgcctgc cctggcgatg acagcgccga ctgcccgatt atcgaaaatc
    33001 tctccggctg ctgtcatcat cgggcagggt gattaagacg agacggctcg gatatgtagg
    33061 gttatccctt ctatcgcaat gacttcaaca tgcgtacctg caccgagatc ctcgctggcg
    33121 ctgacaggcc atgaactgtc accgacgcgc atatgaccgc gcccgttgac cagcggagat
    33181 tccagcacaa aacgtcggcc aatcagctgc tgcccgcgct ggtttaaatg actatcgctg
    33241 tgcttttgtt cgcgcacccg ccgcgacaac catttccacc acagccaggc ggcgagcagc
    33301 gtcaggatgg caaacattac cccttgccac tcccaaccca gcggcaccag ccagaccacc
    33361 aggccggtaa tcaccgctgc cacgccgctc cacaacaaat aaccatttcc gcccagcatc
    33421 tcggctgcca gcagcaaccc gccgagactg agccagaaaa tatgtggatg aacgaccatt
    33481 aactccatca tggctgagtc cgtttgttgg cgctgtcttt caccagctcg gcaatcccgg
    33541 caatcgaccc catcaggctg ctggcctcta atggcatcat cactactttg ctgttactgg
    33601 aggaaccgat ctgctgtaac gcttcggtgt atttttgcgc tacgaagtag ttcaccgcct
    33661 gaatatcacc ggaggcgata gcttcagaca ccattttggt ggcgcgggct tctgcttcgg
    33721 cggaacgttc acgcgcttca gcctgtaaaa acgccgactg acgttcgcct tccgctttca
    33781 ggatttgcga ctgtttttca ccttcggctt tgaggatttc cgcctgacgg atcccttccg
    33841 cttcaagaat gtaagcgcgt ttggtacgtt ccgctttcat ctgcgcgttc attgaagaga
    33901 taagctctgc cggtgggcgc acgtcgcgaa tttcaatacg ggtgacttta atcccccacg
    33961 ggttggtggc ttcatcgaca atacgcagca ggcgtgagtt gatgctgtcg cgctgagaga
    34021 gcatttcgtc aagttccatt gaacccaaca cggtacggat gttagtcatg gtcaggttga
    34081 tgatcgccag ctccagattg ctgacttcat aggcagcgcg tggcgcgtca atcacctgaa
    34141 taaaacagac tgcgtcgatg gtaacgttgg cgttatcttt cgagataact tcctgggaag
    34201 ggatatcgag cacttgctcc atcatattga tcttgcgacc aatgcgatcc ataaacggca
    34261 ccaccagact gagccccggc tgtaacgttt tggtatagcg accaaaacgt tctaccgtcc
    34321 attgatagcc ctgcggcacg attttgacgc ccgcgccgac aatgaccagc gcgacaaaaa
    34381 tgagaatcgg gataaagata agcatcggaa aaacctcctg ttgtaccgtc cataatcagc
    34441 aaaattgctg cttgattaaa caaattatac ctgattactg aaagagagtt cccccttatt
    34501 cctgcgaagg ataaactgtt tttagtaaaa atcagaaaaa gggaacagcg atgcaggaaa
    34561 atagtccttt gcttcagcta caaaacgtag gatatctggc gggtgatacg aagattctta
    34621 ataacatcaa tttttcgctg cgtgctggcg aatttaagtt aattaccggt ccttctggtt
    34681 gtggcaaaag tacgctgcta aaaatagttg cttcattgat cagcccaacc agcggaacgt
    34741 tactgtttga aggtgaggat gtcagcacac taaaaccaga aatctaccgc cagcaagtct
    34801 cttactgcgc ccagacgccg acgctgtttg gcgatacggt atacgataat ctgatctttc
    34861 cctggcagat ccgtgaccag cagcctgacc cggccatttt tctcgatttt ctcgaacgct
    34921 tcgctttgcc ggacagcatt ttgacgaaga atatcgccga gctatctggt ggtgaaaaac
    34981 aacgcatctc attgattcgt aacctgcaat ttatgccgaa ggttttattg ctggatgaaa
    35041 taaccagtgc gctggatgaa agtaataaac ataacgtcaa tgagatgatc catcgttatg
    35101 tgcgcgagca aaatattgcc gtgctgtggg tgacacacga taaagacgaa attaatcatg
    35161 cggataaagt gattacactg caaccgcatg ccggagaaat gcaggaagca cgctatgaac
    35221 tcgcataata ttactaacga atcattagca ctggcattaa tgctggtggt ggtggcaatc
    35281 ttaattagcc ataaagaaaa actggcgctg gagaaagata ttctatggag cgtcgggcga
    35341 gcgataattc agctgattat tgtcggctat gtgctgaagt atattttcag cgtggatgat
    35401 gccagcctga cattattgat ggtgttattt atctgcttta atgcggcgtg gaacgcgcaa
    35461 aaacgcagta aatatattgc taaagctttt atctcatcgt ttattgccat tacggtcggg
    35521 gcgggaatta ccctggcggt gctgattctc tcaggggcga ttgaatttat cccgatgcag
    35581 gtgatccctg tcgccgggat gattgccggt aacgccatgg tagcggtggg gttgtgttac
    35641 aacaatttag ggcaacgggt cattagcgaa cagcaacaga ttcaggagaa actgagtctt
    35701 ggcgcgacgc cgaagcaggc ttcagcgata ttgattcgcg acagtattcg cgcggcttta
    35761 attccgacgg tcgattcagc aaaaacggtt ggcttagtga gtttaccagg aatgatgtcc
    35821 gggctgatat ttgccgggat tgatccggtg aaggcgatta aatatcagat tatggtgacc
    35881 tttatgctgc tctcaaccgc cagcttgtcg accattattg cctgctattt aacctatcgt
    35941 aagttttata attcgcgcca ccagttggtg gtgactcaat tgaagaagaa atgatgcgat
    36001 gccggatgcg cagcatcgca tccggcgttg tggttcatgt gccgaacaac ggaatatcaa
    36061 tacaacaatg catacagctg gcggcgatac ttcgacgcca gtgcatcacc cgtacccagc
    36121 gcagcgagga tctcctggaa cgttttacgc gtctgaccgt ctgcggcggt gagatcttta
    36181 cgcagatgcc cgaacagcaa ctccagcgcc tcttcattgc gcccaacctg atgcagttgc
    36241 agcgccagtt gcgtcgccag tgcggcatct tctggattct ccgccacctg ctgttgcaac
    36301 tgttgaattt ccggcttatc agccgcctgc ttcagcagtt cgatttgcgc taccagcccc
    36361 tggtagcggg tgtcctgatc ctgcaacgga atggttttca gcaccgcttc cgcatcttca
    36421 gaacggttca gcgcaatcag cgtttctgcc agcagcaggc cgatctcccc gttctgattc
    36481 gacaactgcc aggcgtcttt cagcaatggc agggcatcgg tgtaattgcc ttcctgcatc
    36541 agttgcatcg cctgctgcgc tttcagctct tcttcgcgcg gcagcacttt atccagcagg
    36601 gcgcggatcg cctcttccgg ttgcggcccc tggaagccat ctaccggttg cccgttctgg
    36661 aacagataca cggtcggaat cgcgcgcaga ccaaactggg cggcaatcat ctgctccgcg
    36721 tcgcagtcca gcttcgccag aataaattgc ccgttgtact gcgccgcgag gctttccagg
    36781 atcggggtta actgcaaaca gtgctggcta cgttcagacc aaaaatagaa cagcaccgga
    36841 gtggtcatcg actgttcaag aacctgttgc aggttagatt cgttaatgtt gacaatattt
    36901 tctacggaca tggagtcgct ctctgttgtc gatattttct ttgacatggg ggcttaagcg
    36961 cgcgcttcaa ctcacccctg caatattttg tccatcacgc gcccaggcag caggcgctcg
    37021 agcaccatta ctgcccaggt caccagcgtt accggatagc gcatcttcgg cttctcgcta
    37081 ataaaagcat ggcgcacttt gtcgaccacc gcttccggtc ccaacgtaaa gcgggcggcg
    37141 atgccgggat tttcgactgg tttatcactt tgcgtctggt tgacgttgtc ggtgaagcga
    37201 gtacgaatgg gaccgggttc gatcaggctg actttaattc cgctgtggcg tagctccatg
    37261 cgcagcgcgt ctgaccacgc ctccagcgca tatttactgg ccgcgtaagc gccacgaccc
    37321 ggcgtggaga ttaatcccat caccgatgat gtcatcacaa tacgcccttc accgtgcggt
    37381 aacatcgcgg gtaacaggcg catggtgagc tggtgtgcgc cgaaaaagtt ggcggaaaac
    37441 tgctgttcca tctgcgcacg gctgatggtg gaaagggggc catacatgcc gaatccggca
    37501 ttgttaaaga tcccatacag acaattatcg gtcagggcga tcacctcgtc tgctgcgcga
    37561 ttaacacttt ctggtgaatc cagatcgatc aacacgccgg taaatcccat gttgttcata
    37621 cgctcaacat cattcggttt tcggcaacct gccagcacat gaaaaccctg gcgttttaat
    37681 tcgagcgcgc tttccaggcc aattccactg gaacatccgg taattaagac cgatttttgc
    37741 ataactttac ctgtcaggat ctccgttgct ttatgagtca tgatttacta aaggctgcaa
    37801 ctgcttcgcc atccagtcgg caataaacgg ctgggcgtcg cggttgggat gaataccgtc
    37861 atcctgcatc cattgtggct tgaggtagac ctcttccata aaaaagggca gcagcggaac
    37921 atcaaactct ttggcgagtt tggggtaaat ggcgctaaag gcttcattat aacggcgacc
    37981 atagtttgca ggcagacgta tttgcattaa caatggttca gcgttggcgg ctttgacatc
    38041 ctgcaaaatc tggcgcagcg tttgctcggt ttgctgtggc tgaaaaccac gcaaaccatc
    38101 attgcgccca gttcaaccag cacccaacgc ggctgatgct gtttgagcag agccggaagg
    38161 cgcgccagcc cttgttgcga ggtgtcgccg ctgatgctgg cattaactac cgacgtttta
    38221 ctctgccact tatcattcaa caaggcaggc caggccgcgc tggcagacat tcgatacccg
    38281 gcgctcaggc tatcacccag aatcaataac gtgtccgctg cggcggcacg gaaggttaac
    38341 aggaccagga acaggaaggg caaatgccag cggaaaacat tgttgaagtt catcatctta
    38401 agaagtccgt cggtcagggg gagcatgaac tctccatcct caccggagtt gagctggttg
    38461 tcaaacgtgg cgagaccatc gcactggtgg gcgagtcggg atcgggtaag tcaaccttgc
    38521 tggcgatcct cgccgggctt gatgacggca gcagtggcga agtgagtctg gtgggacaac
    38581 cgctacataa tatggacgaa gaagcgcggg caaagttgcg cgcgaagcac gtcggctttg
    38641 tttttcagtt atttatgtta attcctaccc ttaacgcgct ggaaaacgtc gagcttccgg
    38701 ctctgctgcg cggtgagagt agcgcggaaa gtcgtaacgg ggcgaaagcg ttgctcgaac
    38761 agttagggct gggtaaacgt ctggatcatc ttccggcaca gctttccggc ggtgaacagc
    38821 aacgagtggc gctggcacga gcctttaatg gtcgacctga tgtgctgttt gccgacgaac
    38881 ccaccggcaa ccttgaccgc cagacgggcg ataaaattgc cgacctgctg ttttccctca
    38941 accgtgaaca tggcaccacg ttgattatgg tgacccacga cctgcaactg gcggcacgct
    39001 gcgaccgctg cttacggctg gtgaacgggc agttgcagga ggaagcatga ttgcacgttg
    39061 gttctggcgc gaatggcgtt cgccgtcgct attaattgtc tggctggcgc taagcctggc
    39121 ggtggcctgc gtgctggcgc tgggcaatat cagcgatcgc atggagaagg gcttaagcca
    39181 gcaaagccgt gagtttatgg cgggcgatcg ggcgttgcgc agttcacgcg aagtgccgca
    39241 agcgtggctg gaggaagcgc aaaagcgcgg cctgaaagtc ggcaagcagc tgactttcgc
    39301 cacaatgacc tttgcaggcg acacaccgca gctggcgaac gtcaaagcgg tggatgatat
    39361 ctacccgatg tatggcggtc tgcaaactaa tccccctggc ctgaaaccgc aggcgggcag
    39421 cgtattgctg gccccacgcc tgatggcact gcttaacctg aaaacgggcg acaccattga
    39481 cgtgggcgat gccaccttgc ggattgccgg agaagtgatt caggaaccgg attccggttt
    39541 taaccccttc cagatggctc cgcgtctgat gatgaatctg gcggatgtcg ataaaaccgg
    39601 agccgtgcag ccggggagtc gggtcacctg gcgttataaa ttcggcggca acgagaacca
    39661 gctcgacggc tatgagaaat ggttgttacc tcagcttaaa cccgaacaac gctggtacgg
    39721 tctggaacag gacgaaggcg cgctggggcg atcgatggaa cgctcgcaac agttcctgct
    39781 gctttcggcg cttctgacct tgctgctggc agtggcagcg gtggcggtag cgatgaatca
    39841 ttactgtcgc agtcgctacg atctggtggc gatcctcaaa acgctggggg cagggcgagc
    39901 gcaactgcgt aagctaatcg tcggtcagtg gttgatggtg ctgacgcttt cagccgttac
    39961 cggtggggcc ataggcctgt tgttcgaaaa cgtgttgatg gtgctgctca agcccgttct
    40021 gcctgctgca ctaccgccag ccagcctctg gccgtggctg tgggcgcttg gcaccatgac
    40081 ggtcatctcg ctgctggtgg ggctacgacc ataccgcttg ttgctggcaa cgcagccttt
    40141 acgcgtatta cgtaatgatg tggtcgcgaa cgtctggccg ctgaagtttt atctgccgat
    40201 tgtcagtgtg gtggttgtgc tgctgctcgc cggattaatg ggtggcagca tgctgctttg
    40261 ggcggtgctg gcgggcgcgg tagtactggc tttgctgtgc ggtgtgctgg gctggatgct
    40321 gctgaatgta cttcgccgca tgacgctgaa atcgctgcct ctgcgcctgg cggttagccg
    40381 cctgttacgt cagccgtggt caacgttaag ccagctttcg gcattttcgc tctcctttat
    40441 gctgctggca ctgctgctgg tgctgcgtgg cgatctgctc gaccgctggc agcagcagct
    40501 accgccagaa agcccgaact actttttaat taacatcgcc acagaacagg tagcaccgct
    40561 aaaagcgttc ctcgcggaac atcatatagt cccggaatca ttttatccgg tagtgcgggc
    40621 gcggctgacg gcgattaatg ataagccgac agaaggtaat gaagatgagg cgcttaatcg
    40681 cgaactcaat cttacctggc aaaatacgcg gcccgatcat aatccgattg tcgccggtaa
    40741 ctggccgcca aaagccgatg aagtgtcgat ggaagagggg ctggcgaaac gcttaaacgt
    40801 tgccctcggt gataccgtga cttttatggg cgatacccag gagttccgcg ctaaagtgac
    40861 cagcctgcgc aaagtggact gggaaagtct gcggcctaat ttctatttta ttttccctga
    40921 aggggcatta gacgggcaac cgcagagctg gcttaccagt ttccgctggg agaatggcaa
    40981 cggcatgttg acgcaactca accgccagtt cccgaccatt agcctgttag acattggcgc
    41041 gattttaaaa caggtcggtc aggtgctgga gcaggtaagt cgggcgctgg aggtgatggt
    41101 ggtgctggtc accgcctgcg gtatgttgct gttgctggca caggtgcagg tgggaatgcg
    41161 ccagcgtcat caggagctgg tggtatggcg cacactcggt gcggggaaaa aactgctgcg
    41221 cacgacattg tggtgtgatg ttcgccatgc ttgggtttgt ttccggcctg gtggccgcaa
    41281 ttggtgcgga aacggcactg gcagtgttgc aggcgaaagt gtttgatttc ccgtgggagc
    41341 cagactggcg attgtggatt gttctgccgt gcagcggtgc gctgctgctg tcgcttttcg
    41401 gcggctggct gggtgcgcga ctggttaagg gtaaggcgct gttcaggcag tttgtggggt
    41461 gatgacaggt gttagattgc ctgatgcgct acgcttatca ggcctacgaa gagcgtgcaa
    41521 tatattgatt ttgcaagatt gtgtaggccg gataaggcgt ttacgccgca tctggcatga
    41581 acagtgtgca ttttggcaat aaatatgaaa ccaactgtca tgtcggtttt tttaaaggcc
    41641 gaaggaggta aaccttcggc cttagctacg tttcctcagt tattcaataa actgacggat
    41701 cagttccagc agcgcgatta gcgccttaag aacgatgaga actttatcga gaaaagtttt
    41761 catccgttgc tcccttaagg gagaaagaca atcacagctt acccttacgt tgcctataac
    41821 tatgattttc ttcatgtgct agatgaggac tgtctctctc caaattagag caccagaatc
    41881 agcaccacct gtatcctgac caggacttaa aagtactttt ccgcactgcg gtaatccctt
    41941 gaggcagtat cactactagc gtcgggattg tatcgccaac atagctgttt gaatatgtag
    42001 gcatcctgac ttataattca cttgctagat gaggactgtt taatttctgc ccggaaccac
    42061 caagtcttgg gcggaaaaaa taaaaaaacc gactgtcatg tcggtttttt tatatctgta
    42121 ttctggtgca accattttgt tctcacgaca atcgccgttt cagaataccg cccatcactg
    42181 cgccttaacc cataccgcca cttccgccca ctcaccgcga aagacaactt tttccgcttt
    42241 tttctcaagc tgatagcgat acattgggtc gtaatattct ttaagtaacg gcaccagcca
    42301 ggccagatga ccgtcggtgc tgccggtggt gagttgcgtt gtcagcgccg tatccagctg
    42361 ggcagcgagt tcgttatagc gctgtaaccc cagccgacgc ttaatcgccg aaagtccgtg
    42421 atgcaggtat tcgcaatatt cctgccagcc ctgttcgtcg ccataagcgt gggtaaaatc
    42481 atgatgcata cgcaagaaat actcttcatt caggcgttca agacggatct caaacggatc
    42541 ttctaccacc gcaatcgccg cctgagtcat tcgctcgcgc agacattccg gcaggtgatt
    42601 cgaaccgatc atccggcttt catcttccag cacccacagg cgcaaatcct gacgggcgtc
    42661 ggtttttagc atttcggcag ccagcaggtt ttcaaaactc gcctggctaa gttgtggttg
    42721 taacgtgcga ccaaacgccg aaccgcgatg acgcgccagc ccttccagat caacaccgtt
    42781 tggctgttgt tgcactaata gcgttttacc gctgccggta caaccgccaa tcagcactat
    42841 cggtttttgt gccagttcaa tggtcgcctg aatcgcggtc tggcgcagtg ccttataacc
    42901 gccttccacc agcggataat caatccccgc ttcatgcagc cagcgttgca caatatgtga
    42961 acgttgaccg ccacgggcgc agcagagaat accttgcgga ttttgcaggc acgctgcccg
    43021 ccaggcgtac atacgctgct gacgaatttc acccgccacc agtttatgcc ccagcgccag
    43081 cgctgcgtct gagccttgct gtttatagca ggtgccaacg gaggcgcgtt catcgttatc
    43141 cattaacggc agattgatag cggcgggcat tgcgccttgc tcaaactcga taggggcgcg
    43201 aacatcaata atgggcgtat cagcaatcag cagggcacga tagtcctgtt ccgtgtgtct
    43261 ctcttgcata gttaaaagtg aacctcaaat cagcttgcgc gctattttac gcgccaacgc
    43321 gcaaggaaac ttgattttta actgcgtggg gtgccgaaaa tttctaaaaa tccgctgatt
    43381 tccggcctgt gttgggtaaa caaggtcaca atatcttcta ccgctttgca gctgccaaat
    43441 ttgcgccatg ccagactcaa tggcgaagga gggcgcatcg ttgggattac ccggctgacc
    43501 agttgttgat tatcgatcat tgactggcaa agtgattttg gcaaaaaacc aatgccaaca
    43561 cccgccagat gggcggcgat tttcgtttcc atatcgggaa caataatctc tttttgcccc
    43621 ggcaatcgcc aggcgacgcg tttggttaag gtgccggcgc tgtcttcaat attgaccgcc
    43681 ggaaagcgac gcaactgcgc ttctgttagc ggctcttcaa cgttcgccag cggatgatcc
    43741 gccgccatga caaatcgcca ttgcaccgat cctaagggat caagactaaa ggtatttgcc
    43801 agcgcctcag ttcccgtgac cccgatagcc agcgaaaaac cttcgtacaa tagcgagtcc
    43861 cagacgccca tatagatttg tcgggagatg tgaaactggg taaaggggta acgctcattc
    43921 agccacgcca gcaactgggc gacggcctgg gggttataga gcaggttgtt gatgacaata
    43981 ttcacctggc gttccacgcc atcattcacc tgttgcagct cgctgggcat actttccagc
    44041 cagctcagcc agtctctggc ctgggaaagt agatgctcgc cagccgctgt caacgttacg
    44101 ctacgagtcg tacggaaaaa cagcgctact ccggtattct cttccagaag tttaatgcga
    44161 taactgatcg tcgccgtggt tttacataat cgttctgccg cttttgaaaa acttcctgtt
    44221 tcagcaaccg caatgaaagt ccgcaaggtt tctggatcga acatcttcag gtatcccctt
    44281 ttaaatccgc aagttgcgtg attttcttat cctctgattt atcagtattt ttacatgata
    44341 atcctgttca atttgtggac taaatctagt tttggaaaaa cattccaact tttgtattaa
    44401 tgttgttctc ttaaggtttt agattgcctg ttattgaaac caagctgacc ggtcggcggt
    44461 ggttgaacgg aattatgtta caaggacaaa aagatgaaac ttcaggtatt accgttaagt
    44521 caggaagcct ttagtgctta tggcgacgta atcgaaacgc agcaacggga ttttttccat
    44581 attaacaatg gcctggtgga gcgttaccac gatttggcgc tggttgagat tcttgagcaa
    44641 gaccgtacgc ttatcagcat taaccgcgcg caaccggcga atctgccgct gaccattcac
    44701 gaactcgaac gtcatccgct gggcattcag gcctttatcc caatgaaagg tgaagtgttt
    44761 gtggtggtcg tggcgttagg cgacgacaaa ccagacctgt caacgctgcg ggcgtttatc
    44821 accaacggtg aacagggggt gaattaccat cgtaacgtct ggcatcaccc acttttcgcc
    44881 tggcagcgcg tcaccgattt tctgaccatc gatcgcggcg gcagtgacaa ctgtgatgtt
    44941 gaaagtattc ctgaacagga actctgtttt gcgtgacgcc tgcaaccgac ttgcataaga
    45001 taaactaatt gttcattgtt tatgctcact tgtaggtcgg agttaacgta ggtatgacgg
    45061 aagttagacg gcgcggcagg ccaggacagg cggagcctgt ggcacagaag ggcgcacagg
    45121 cgttagagcg gggaattgcg attctgcaat atctggaaaa aagtggggga agttcgtcgg
    45181 ttagcgatat ttctctcaat ctggatttgc cgctctccac gacctttcgc ttgctgaagg
    45241 ttttacaagc agcggatttt gtctatcagg acagtcaatt aggctggtgg catataggat
    45301 taggtgtctt taacgtcggt gcggcgtaca tccataaccg cgatgtcctc tccgtcgccg
    45361 ggccgtttat gcgccgcctg atgttacttt ccggcgaaac ggtcaatgtc gcgatccgta
    45421 acggcaatga agcggtatta attggtcagt tagagtgtaa atcgatggtc aggatgtgtg
    45481 cgccactggg cagtcgtctg ccgctgcatg cttccggtgc gggcaaagcg ctgctttatc
    45541 cgctggcgga agaggagttg atgagcatca ttctgcaaac cggtttgcag cagtttacgc
    45601 caactacgct tgtggatatg cccaccttgc tgaaggacct ggaacaagcg cgtgaactgg
    45661 gctataccgt agataaagaa gagcatgttg taggtctgaa ttgcatagct tcagcaattt
    45721 acgatgatgt cggtagagtt gttgccgcta tctccatctc cgggccttca tcaagactga
    45781 cagaagatcg ttttgtcagt cagggtgagc tggtcagaga caccgcccgc gatatcagca
    45841 cggcgttggg actgaaagca catccatagt gtctgtcgca tcccgctctg cggagcgggt
    45901 ttttttgaca aaatttgaaa gttggaaaat ttttccaact aatagaggta ggaataaaat
    45961 ggcaaaaatg agagccgttg acgcggcaat gtatgtgctg gagaaagaag gtatcaccac
    46021 cgccttcggc gttccgggag ctgcaattaa tccgttctac tcagcgatgc gtaagcacgg
    46081 cggtattcgt cacattctgg cgcgtcatgt ggaaggtgtt tcgcacatgg cggaaggtta
    46141 tacccgcgca acggcaggga atatcggcgt atgtctgggg acttccggtc ctgcgggcac
    46201 ggacatgatc accgcgctct attccgcttc tgctgattcc attcctattc tgtgcattac
    46261 cggccaggca ccgcgcgccc gtctgcataa agaagatttt caggccgtag atattgaagc
    46321 aattgctaaa ccggtcagca aaatggcggt tacagttcgt gaagcggcgc tggtgcctcg
    46381 cgtgctgcaa caggcatttc acctgatgcg ttctggtcgt ccgggtccgg tactggtgga
    46441 tttaccgttc gacgttcagg ttgcggaaat cgagtttgat cctgacatgt acgaaccgct
    46501 gccggtctac aaacctgctg ccagccgtat gcagatcgaa aaagctgtag aaatgttaat
    46561 ccaggccgaa cgtccggtga ttgttgccgg gggcggggta atcaatgctg acgcagctgc
    46621 actgttacaa cagtttgctg aactgaccag cgttccggtg atcccaacgc tgatgggctg
    46681 gggctgtatc ccggacgatc atgaactgat ggccgggatg gtcggtctgc aaaccgcgca
    46741 tcgttacggt aacgcaacgt tgctggcgtc cgacatggtg tttggtatcg gtaaccgttt
    46801 tgctaaccgt cataccggtt cggtagagaa atacaccgaa gggcgcaaaa tcgttcatat
    46861 tgatattgag ccgacgcaaa ttggtcgcgt gctgtgtccg gatctcggca ttgtctctga
    46921 tgctaaagcg gcgctgacac tgctggttga agtggcgcag gagatgcaaa aagcgggtcg
    46981 tctgccgtgt cgtaaagaat gggtcgccga ctgccagcag cgtaaacgca ctttgctgcg
    47041 caaaacccac ttcgacaacg tgccggtgaa accgcagcgc gtgtatgaag agatgaacaa
    47101 agcctttggt cgcgatgttt gttatgtcac cactattggt ctgtcacaaa tcgctgcggc
    47161 acaaatgctg catgtcttta aagaccgcca ctggatcaac tgtggtcagg ctggtccgtt
    47221 aggctggacg attccggcgg cgctgggggg ttgtgccgct gatccgaaac gcaatgtggt
    47281 ggcgatttct ggcgactttg acttccagtt cctgattgaa gagttagccg ttggcgcgca
    47341 gttcaacatt ccgtacatcc atgtgctggt gaataacgcc tatctcggcc tgattcgtca
    47401 gtcacaacgc gcttttgaca tggactactg cgtgcaactc gctttcgaga atatcaactc
    47461 cagtgaagtg aatggctacg gtgttgacca cgtaaaagta gcggaaggtt taggttgtaa
    47521 agctattcgg gtcttcaaac cggaagatat tgcgccagcc tttgaacagg cgaaagcctt
    47581 aatggcgcaa tatcgggtac cggtagtcgt ggaagttatt ctcgagcgtg tgaccaatat
    47641 ttcgatgggc agcgaactgg ataacgtcat ggaatttgaa gatatcgccg ataacgcagc
    47701 ggatgcaccg actgaaacct gcttcatgca ctatgaataa gggagataaa tgatgttacg
    47761 tttctctgct aatttatcga tgttatttgg tgaatatgat tttctcgccc gttttgagaa
    47821 agctgcgcag tgtggttttc gcggcgttga atttatgttt ccttatgact acgacattga
    47881 agaattaaaa caggtactgg cgagtaataa actcgaacat acgctgcaca atttaccggc
    47941 aggtgactgg gcggcgggcg agcgcggtat tgcctgtatt cctggccgtg aagaagagtt
    48001 tcgggatggc gtagcagcag cgatt
//
LOCUS       DAZPAB010000009        47363 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZPAB010000009 DAZPAB010000000
VERSION     DAZPAB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.009"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            69..551
                     /gene="sseA"
                     /locus_tag="ACHUPG_000459"
                     /pseudo
     CDS             69..551
                     /gene="sseA"
                     /locus_tag="ACHUPG_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
     gene            1213..1338
                     /gene="timP"
                     /locus_tag="ACHUPG_000460"
     CDS             1213..1338
                     /gene="timP"
                     /locus_tag="ACHUPG_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR0860214.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(1370..2146)
                     /gene="sseB"
                     /locus_tag="ACHUPG_000461"
     CDS             complement(1370..2146)
                     /gene="sseB"
                     /locus_tag="ACHUPG_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR0860215.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2288..3571)
                     /gene="pepB"
                     /locus_tag="ACHUPG_000462"
     CDS             complement(2288..3571)
                     /gene="pepB"
                     /locus_tag="ACHUPG_000462"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR0860216.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3630..3830)
                     /gene="iscX"
                     /locus_tag="ACHUPG_000463"
     CDS             complement(3630..3830)
                     /gene="iscX"
                     /locus_tag="ACHUPG_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR0860217.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3842..4177)
                     /gene="fdx"
                     /locus_tag="ACHUPG_000464"
     CDS             complement(3842..4177)
                     /gene="fdx"
                     /locus_tag="ACHUPG_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR0860218.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4179..6029)
                     /gene="hscA"
                     /locus_tag="ACHUPG_000465"
     CDS             complement(4179..6029)
                     /gene="hscA"
                     /locus_tag="ACHUPG_000465"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR0860219.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(6046..6561)
                     /gene="hscB"
                     /locus_tag="ACHUPG_000466"
     CDS             complement(6046..6561)
                     /gene="hscB"
                     /locus_tag="ACHUPG_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR0860220.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6657..6980)
                     /gene="iscA"
                     /locus_tag="ACHUPG_000467"
     CDS             complement(6657..6980)
                     /gene="iscA"
                     /locus_tag="ACHUPG_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR0860221.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6997..7383)
                     /gene="iscU"
                     /locus_tag="ACHUPG_000468"
     CDS             complement(6997..7383)
                     /gene="iscU"
                     /locus_tag="ACHUPG_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR0860222.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7411..8625)
                     /gene="iscS"
                     /locus_tag="ACHUPG_000469"
     CDS             complement(7411..8625)
                     /gene="iscS"
                     /locus_tag="ACHUPG_000469"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR0860223.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8737..9225)
                     /gene="iscR"
                     /locus_tag="ACHUPG_000470"
     CDS             complement(8737..9225)
                     /gene="iscR"
                     /locus_tag="ACHUPG_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR0860224.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9495..10235)
                     /gene="trmJ"
                     /locus_tag="ACHUPG_000471"
     CDS             complement(9495..10235)
                     /gene="trmJ"
                     /locus_tag="ACHUPG_000471"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR0860225.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10354..11157
                     /gene="suhB"
                     /locus_tag="ACHUPG_000472"
     CDS             10354..11157
                     /gene="suhB"
                     /locus_tag="ACHUPG_000472"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR0860226.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11302..12156
                     /locus_tag="ACHUPG_000473"
     CDS             11302..12156
                     /locus_tag="ACHUPG_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0860227.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12347..13627
                     /gene="csiE"
                     /locus_tag="ACHUPG_000474"
     CDS             12347..13627
                     /gene="csiE"
                     /locus_tag="ACHUPG_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR0860228.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13619..14758)
                     /locus_tag="ACHUPG_000475"
     CDS             complement(13619..14758)
                     /locus_tag="ACHUPG_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR0860229.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14918..15808)
                     /gene="hcaR"
                     /locus_tag="ACHUPG_000476"
     CDS             complement(14918..15808)
                     /gene="hcaR"
                     /locus_tag="ACHUPG_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR0860230.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15944..17305
                     /gene="hcaE"
                     /locus_tag="ACHUPG_000477"
     CDS             15944..17305
                     /gene="hcaE"
                     /locus_tag="ACHUPG_000477"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR0860231.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17302..17820
                     /gene="hcaF"
                     /locus_tag="ACHUPG_000478"
     CDS             17302..17820
                     /gene="hcaF"
                     /locus_tag="ACHUPG_000478"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR0860232.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17820..18140
                     /locus_tag="ACHUPG_000479"
     CDS             17820..18140
                     /locus_tag="ACHUPG_000479"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR0860233.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18137..18949
                     /gene="hcaB"
                     /locus_tag="ACHUPG_000480"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18137..18949
                     /gene="hcaB"
                     /locus_tag="ACHUPG_000480"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR0860234.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18959..20176
                     /gene="hcaD"
                     /locus_tag="ACHUPG_000481"
     CDS             18959..20176
                     /gene="hcaD"
                     /locus_tag="ACHUPG_000481"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFR0860235.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRSAAQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVV
                     DGEKVELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIF
                     AGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQ
                     FIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDA
                     KLLIDENIALKSL"
     gene            20273..20695
                     /locus_tag="ACHUPG_000482"
     CDS             20273..20695
                     /locus_tag="ACHUPG_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR0860236.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20743..21615)
                     /locus_tag="ACHUPG_000483"
     CDS             complement(20743..21615)
                     /locus_tag="ACHUPG_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR0860237.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21627..22721)
                     /locus_tag="ACHUPG_000484"
     CDS             complement(21627..22721)
                     /locus_tag="ACHUPG_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR0860238.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22754..23752)
                     /locus_tag="ACHUPG_000485"
     CDS             complement(22754..23752)
                     /locus_tag="ACHUPG_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0860239.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23777..25288)
                     /locus_tag="ACHUPG_000486"
     CDS             complement(23777..25288)
                     /locus_tag="ACHUPG_000486"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR0860240.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25311..26293)
                     /locus_tag="ACHUPG_000487"
                     /pseudo
     CDS             complement(25311..26293)
                     /locus_tag="ACHUPG_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26390..29671)
                     /locus_tag="ACHUPG_000488"
                     /pseudo
     CDS             complement(26390..29671)
                     /locus_tag="ACHUPG_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            29789..30982
                     /locus_tag="ACHUPG_000489"
     CDS             29789..30982
                     /locus_tag="ACHUPG_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFR0860241.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31180..32433)
                     /gene="glyA"
                     /locus_tag="ACHUPG_000490"
     CDS             complement(31180..32433)
                     /gene="glyA"
                     /locus_tag="ACHUPG_000490"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR0860242.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32760..33950
                     /gene="hmpA"
                     /locus_tag="ACHUPG_000491"
     CDS             32760..33950
                     /gene="hmpA"
                     /locus_tag="ACHUPG_000491"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR0860243.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33995..34333)
                     /gene="glnB"
                     /locus_tag="ACHUPG_000492"
     CDS             complement(33995..34333)
                     /gene="glnB"
                     /locus_tag="ACHUPG_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR0860244.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34394..35728)
                     /gene="glrR"
                     /locus_tag="ACHUPG_000493"
     CDS             complement(34394..35728)
                     /gene="glrR"
                     /locus_tag="ACHUPG_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR0860245.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35718..36431)
                     /gene="qseG"
                     /locus_tag="ACHUPG_000494"
     CDS             complement(35718..36431)
                     /gene="qseG"
                     /locus_tag="ACHUPG_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR0860246.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36596..38023)
                     /gene="qseE"
                     /locus_tag="ACHUPG_000495"
     CDS             complement(36596..38023)
                     /gene="qseE"
                     /locus_tag="ACHUPG_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR0860247.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38581..42468)
                     /gene="purL"
                     /locus_tag="ACHUPG_000496"
                     /gene_synonym="purI"
     CDS             complement(38581..42468)
                     /gene="purL"
                     /locus_tag="ACHUPG_000496"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR0860248.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42727..44283
                     /gene="mltF"
                     /locus_tag="ACHUPG_000497"
     CDS             42727..44283
                     /gene="mltF"
                     /locus_tag="ACHUPG_000497"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR0860249.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44280..44783)
                     /gene="tadA"
                     /locus_tag="ACHUPG_000498"
     CDS             complement(44280..44783)
                     /gene="tadA"
                     /locus_tag="ACHUPG_000498"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR0860250.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(44841..45476)
                     /gene="yfhb"
                     /locus_tag="ACHUPG_000499"
     CDS             complement(44841..45476)
                     /gene="yfhb"
                     /locus_tag="ACHUPG_000499"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR0860251.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45685..46533
                     /locus_tag="ACHUPG_000500"
     CDS             45685..46533
                     /locus_tag="ACHUPG_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR0860252.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46872..47363)
                     /locus_tag="ACHUPG_000501"
                     /pseudo
     CDS             complement(46872..47363)
                     /locus_tag="ACHUPG_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11295 a        12974 c        11919 g        11175 t
ORIGIN      
        1 gggggcagta atcatgccgg gtctggctgg ctaacacccc attataaggg gtacgaataa
       61 agtaacgggg acttgcaggc tggcagcgcg atgacctgct attagaagaa ggtgcagtag
      121 agctgccgga aggagagttt aacgccgcgt ttaatcctga agcagtggtg aaagtaaccg
      181 atgtgttatt ggcaagccat gaaaatacgg cgcaaattat tgatgcccgc ccggctgcac
      241 gttttaacgc agaagttgat gaacctcgcc caggtttacg tcgcggacat attcccggtg
      301 cattgaacgt tccgtggacg gaactggtgc gcgaaggcga actaaaaacg accgatgaac
      361 tggatgcgat attttttggt cgcggcgtca gctatgacaa accgattatc gtcagctgcg
      421 gctctggtgt aacggcagcc gtggttttgt tagcactcgc gacgctggat gtgccaaacg
      481 tgaaactgta cgacggcgca tggagtgaat ggggcgcgcg ggcagattta ccggttgagc
      541 cagtgaaata agtatattac aggcaataaa aaaccgccga atttggcgtt tttttattgc
      601 tggtctggtt cgcggccttt ccagcaggtt gacttgtgtt acatgagcaa cgcaggtgct
      661 tcacagcaaa acaatactca ccagtaactc tctttttgtc aagcaaaaga gagtaattat
      721 tgtttattta gcgtattatc gacaccggcc ctttccgccg tgttcggtaa taaaataacc
      781 tggcttatta gtccgaattc agacaaatat aaacaaatcc tgctcaaaat taaaaattct
      841 aaccggtaaa agatattact taaacatgta aatttacttt cctttaaaaa acaaaaaacc
      901 gccaaaatca ggcggttatt tgttgctggt ccggttcgcg gcctttccag caggttgtat
      961 taccgtagta atgcaagcgc gtctcagcgg agacaatact cgccagtaac tctctttttg
     1021 tcaagcaaaa gagagttatt attgttctgt tagtgtatta tccactgcgg ccctttccgc
     1081 cgtctcgcaa gcgggcgctg gcttaaggaa aggatgttcc gcgaccgtta gagcaagcgc
     1141 gtctcagcgc ttgctatcgc ggcaatatcg ccagtggtgc tgtcgtgatg cggtcttcgc
     1201 atggaccgca caatgaagat acggtgcttc tgtatcgtac ttattgtttc tggtgcgctg
     1261 ttttcccaag taaataataa ccggagtctc tccggcgaca atttactggt ggttaacaac
     1321 cttcagagca gcaagtaagc ccgaatgccg ccctttaggg cggcatattt tagattatcc
     1381 ggttttgttt aaagtcacgc aaaaaaccac cccagcgacg ttcatagaat ggcgcaatat
     1441 gttcggtaat aaagtggcta attccttttt cccctttttt cacctgacaa atatcgattg
     1501 gttcatcgcc aggtaatgta tcggtcgcta cacttcccgt cgcctgaata atttcttcga
     1561 tatcaccatc ggcttcaatg ccaataagta aattaggctg tgcctcttcg ttctctttaa
     1621 ttgaacaaat aaaagcacgc ttcaccggct taatggtttt aaataaggtg gtgagtgaat
     1681 caatcatttg tgctggcggc tctgcgactt ccgataatat cagcgattca ccgccttcca
     1741 ggatttcctg gctgctcagc ggatttccct cttcaccaat caacaaactg atttcacgcg
     1801 gcataaattc tttaccggtc ggcagtttgg cattaaggaa gagcgtttcg ccaagggtca
     1861 tctcaaacaa tgtgcgaacg ggcattacga caaatgcctg ttcgtcttca accgcctgtt
     1921 gaagcgcttc taacgaggtg aaaaaaggaa tgacgctggt gccgtcttct ttttcccagt
     1981 gctgtaaatc aagcgcacta tcttcaacca cagcctcgcc ctgcgccgcc gtaccaggca
     2041 cccagacggt ggattccagt agagtacgga aaaaggccgg gcggtgcgcc ggttcggttg
     2101 ctgctttttc cagcaggtct tcaagttcgt tttttgtttc ggacataaga accacaattc
     2161 attcaacgtt cggggcgcaa atgcctgatg cgctacgctt atcaggccta caagggattc
     2221 gcaatttgtt gaatttgcag aatttgtagg ccggataagg cgtttacgcc gcatccggca
     2281 tattagttta cgccgttaac agattagcta tcgtgcgcac accaagtccc gtagcgcccg
     2341 cagaccactg ttcaaccggc gctttacggt aagtcgccga gcagtcgata tgcagccagc
     2401 cttgctgata gttctcaaca aagtgcgaca ggaagcccgc cgccgtgctc gcgcctgccg
     2461 gatacgccgc gcttccggta ttgttcagtt cggcaaagtt agacggcagc tggctgcggt
     2521 ggaattccgc cagcggcaga cgccagaatg gttcgttctc ttgtgaggca ctcgccagta
     2581 aacgaccggc aagcgcatcg tcaaaactga acagcgcgtg ataatcatta cccagcgcag
     2641 ttttcgccgc cccggtgagg gtcgccgcat caatgatcat ttccggtttc tgcgcactgg
     2701 catcaatcag accatcggca agcaccagac gcccttcggc atcagtgttc atcacttcaa
     2761 cttttttacc gttgcgatag gtgatgatat cgcccagctt gaacgcattg ccgctaatca
     2821 ggttatccgc acagcagagg aacagcttca cgcgcttgtt cagtccgcgc ataatggcaa
     2881 atgccagcgc cccggtaacc gttgccgcgc cgcccatgtc cgacttcatc gagtccataa
     2941 acgcagtctg tttgatgctg tagccgccgg agtcaaaagt gataccttta cctaccaggc
     3001 acgcgtacac cggtgcttct ttatcgccag ttgggttgta atccagcgcc agcaataccg
     3061 gagaacgttc tgaaccgcgc ccgacagtgt gcagccccat ataaccttgc tcacgcagat
     3121 cttcgccttt ggtgatccga taagtcacac gatcgcccgc gacgttgctg atcagatcaa
     3181 cagcacgctg tgccagttgc gatggtccca attcttctgc cggtgcgttg atggtgtcac
     3241 gcacccagtc gatgatcatc aggcggttat ccagttcctg gcgctgggca tcgtccagat
     3301 ccggccactc cactttacgc gtgcctttcg ggcctttata accttgccag aatgcccagc
     3361 agcgatccgc atcccagcct tcaccgctta actgaacatg cttgatgccc agaccgtcaa
     3421 tcttgcgggc cgcacgctgg atcagcccca gatcgtctgc cccgttcaaa tgcagggtaa
     3481 tgccgtcatt attaatgctg taagttgctt tttctcccca gcgcgcgtcg gcaggttggg
     3541 tagagagggt aatcttcatc gcttctgtca ttttagttat ccttattcgt ttaacagcaa
     3601 aacgggctac ccccaggcag cccgttcatt cactcggctt cgtccagcca gactaacaaa
     3661 atcgcttcga ggattttctc gttggatgcc tgcgggtcgt cgtcgaaatc ttccagatcg
     3721 caaatccact gatgcatatc ggtgaatcga accgttttcg gatcaagatc gggatacgca
     3781 tcgtacagtg cttcgccaat ttcgcggcta tcggtccact taagtcccat actaacctct
     3841 gttaatgctc acgcgcatgg ttgatagtgt aacgcgggat ttcgaccact aaatcttcgt
     3901 cggtgacgcg cgcctggcag cttaaacggc tttccggctc cagtccccag gctttgtcca
     3961 gcatgtcgtc ttcctgctct gagctttccg gcagtgagtc aaaaccttca cgaacgatgc
     4021 agtggcaggt ggtgcaagca caggattttt cacaggcgtg ttcaatctca ataccgttac
     4081 gcagcgctgc gtcgagaatg gtttcaccgc tattagcttc cagaacagcg ccatcagggc
     4141 agaggtcctg atgaggcaaa ataacaatct ttggcatatt aaacctcgtc cacggaatgg
     4201 cctttcagcg cacgacgaac cgactggtcc atgcggcgag cggcgaaatc ctgggtttgt
     4261 ttgtctacgt ttttaatcgc ttgttcgatg gcgtcaacat catcgccctg cgccacttca
     4321 ctcaggtgag cggcagcatt gtcaatgacc tgacgctctg cggcgcttaa cagcgcggca
     4381 tcagcagcca gcgcgccgtg cagactttcc agcacacgcg ccgcttctac tttttgttct
     4441 gccagcatac gggcttttac gtcctgctcg gcatagctca ttgagtcttt gatcatcgaa
     4501 gcgatttcgc tatcggtcag accgtaagac ggtttgacct gaatagacgc ctcaacgccg
     4561 gtggatttct ccatcgccgt cacgctcaaa agaccgtcgg catcgacctg gaacgtcacg
     4621 cgaatatgcg caccgccagc cggtagcgcc ggaataccac gcagcgcaaa acgcgccagt
     4681 gagcggcagt cctgcaccag ttcgcgctca ccctgcatta catggataga catcgccgtc
     4741 tgaccatctt taaaggtggt gaaatcctga gcgcgggcca ccggaatagt ggtattacgc
     4801 ggaatcactt tctccaccag gccgcccatc gtttcgaggc ccagcgacag tgggatcaca
     4861 tcaagcagca gcatttcgct gtctggcttg ttacccacca gaatatccgc ctgaatcgcc
     4921 gcgccaatag cgacgacttt atccgggtcg atggaagtca gcggtggacg accgaaaaat
     4981 tcgcctaccc gttcacgcac cagcggcacg cgagtagaac cgcccaccat caccacttcc
     5041 agcacttcgt cagcttctac acccgcgtct ttcagcgcgc gacgacaagc cagtaaggtt
     5101 cgttttacca gtggcgcgat cagttcattg aattgttcac ggctgatttc gccctgccag
     5161 cccgcaacgt taacggtcac ggagtccgca tcgctcagcg cgattttggc tgcaatggtg
     5221 gcatccagca gttcacgctg aacgcggtta tcgctacgat caggaatgtc cgcctgctcg
     5281 cgaatgtaat ccgccagcag atggtcgaaa tcatcgccgc cgagcgcgga atcaccgccg
     5341 gttgccagca cttcaaacac gccgcgactt aagcgcagaa tggaaatatc aaacgtcccg
     5401 ccaccgaggt cataaacagc gatcacgcct tcctgaccgg aatccagccc gtaggcgata
     5461 gccgcagcag tcggttcgtt aagtaagcgc agaacatgca accccgccag acgcgccgcg
     5521 tctttggtgc cctgacgctg ggcatcgtca aagtacgccg gaacggtgat aactacacca
     5581 tccagctcgc ctgccagggc ttcagttgcc cgcgccgcca gtgctttgag gatgtccgca
     5641 gaaacgcgca ccgggttcag tagccccgcc gccgtttcaa tcatcggcag gccgttttcg
     5701 ctggcctgga attgataagg cagatgcgga tagcgttgct ggatatcagc cagcgagcgc
     5761 cccatcaggc gtttaacaga actaatggtg ttggcggtgt caagcgctgc gttagtacgc
     5821 gcgtcataac ccaccgaatg cccttgctgt tgatagtgaa caacagatgg cagcaggtga
     5881 cggccttcat ggtcggctaa cgtttcggcc tgaccgctgc gcactgtcgc caccagcgag
     5941 ttggttgtgc ccaggtcaat accagccgcc agacgacgct gatgcggcgc agcactcaaa
     6001 ccaggttcac taatttgtaa taaggccatg tttagcttcc agaaattaaa aatcgagcag
     6061 tttttcttcg agttgttcgg cactgctccg cagtttatcg agaaaacgca gcttacgcac
     6121 cgtatccgcc gccgcgtccc acgcctcgtt gtctaactgt tcaaccatca actgatggcg
     6181 ggtatcaaac atctttttca cacgtttgat aaagctttcc agccgcgctt catctttcgc
     6241 ctgttcgatc tcgtccagct cttcgcgcag ctccaactgt tccatcagga acgcggtgtc
     6301 gcgcacagta tgctgctcgc tggcgagatc aaaaccgtgt aaagaaagca aatattccgc
     6361 gcgcattaac ggatgacgca gcgtttgcca ggcctggtta atggttgcag attgctgtac
     6421 ggcggcgagt tgttccgcca ggcttccgct ggcgaattta tcaggatgat actgacgttg
     6481 tagatcctga aaacgcaggc tcagtgcctg ggtatcgagt tgataacggg caggcaagcc
     6541 aaagagggtg aagtaatcca taacattctc agggctgcga tccgccgcga taacggcgaa
     6601 tcaggtaaaa caaaccccac gcgcaggcga ccacggtggg gttatcggta tgcgcatcaa
     6661 acgtggaagc tttcgccgca accacactca tctttaacgt tcgggttggt gaatttaaac
     6721 ccttcattca ggccttcttt tacgaagtcc agctgcgtac cgtccagaaa ttgcaggctt
     6781 ttgccatcga ccacgacttt cacgcctttg tcttcaaaca cgatgtcttc cggcgtcggt
     6841 tcgtcaacaa attccagtac ataagccata cctgaacatc cggaggttct cacgcccaga
     6901 cgcaggccaa accctttgcc gcggttagcc agaaaggtat ttactcgcgc tgctgcactg
     6961 tcgctcagtg taatcgacat aaccaaacct caactcttat tttgcttcac gtttgctttt
     7021 atagtccgca atggcggctt tgatcgcgtc ttctgccaga atagaacagt gaattttcac
     7081 cggcggcagt tcaagttctt cagcaatatc ggtgtttttg atcgcctgcg cttcgtcgag
     7141 agacttccct ttcacccatt cggtgaccag ggagctggaa gcgatagcgg aaccgcagcc
     7201 gtaagtttta aaacgcgcgt cttcaatgat accttcatcg ttgactttaa tctgcaactt
     7261 cattacgtcg ccacaggccg gtgcacccac catgccgctg ccgacgttct cgtcgttgtt
     7321 atcaaaggaa cccacgttac gcggattctc gtaatggtcg ataacttttt cgctgtaagc
     7381 cattataaat tctcctgatt ccgataccga ttaatgatga gcccattcga tgctgttcag
     7441 atccacgccc tgcttgtaca tttcccacag cggagaaagg tcacgcagac gaccgatgga
     7501 tttacgaact aactcgatgg tgtagtcgat ctcttcttca gtagtaaaac gacctaaaga
     7561 gaaacggata gagctatgtg ccagctcgtc gttcagcccc agcgcgcgca gcacgtagga
     7621 cggttcgagg cttgctgacg tacaggcgga acctgaagaa actgcgaggt ctttcagcgc
     7681 cataatcagc gactcacctt caacgtagtt gaagctgacg ttgagaatgt tcggcgcacc
     7741 gtgttccagg tcaccgttca ggtaaacttc ttcgatatct ttgatgccgt tccacagacg
     7801 gttacgcagg ccgcgcagac gttccatctc ggtcgccatc tcttcttttg cgatgcgata
     7861 ggcttcgccc attccgacga tctggtgaac aggcagagtg ccggaacgca taccgcgctc
     7921 gtgaccgccg ccgtgcattt gcgcttcgat gcgtacacgc ggtttacgac gcacatacag
     7981 cgcaccgata cctttcgggc catagatttt gtgaccggag aaagacatca ggtcaacttt
     8041 caactggctc aggtcgattg gcagtttgcc cacgctctgg gttgcatcaa cgtgatagat
     8101 aatgccacga gcacggcaca tttcgccgat agccgcgata tcctgcacca cgccgatttc
     8161 gttatttacg tgcatgatgg aaacaaggat ggtgtcgtca cgcatcgccg cttcgagttc
     8221 tttcaggtcg ataataccgt tacgctgcgg tgccaggtag gtgacttcga aaccttcgcg
     8281 ctccagctga cggcaagtat ccagtaccgc tttgtgttcg gttttgctgg tgatgatgtg
     8341 cttgcctttt ttctgataaa agttggctgc acctttgatc gccaggttgt cagattcggt
     8401 tgcaccagag gtaaagacga tttcacgcgg atcagcgccg accagatcgg caatctgatt
     8461 acgggcgata tctaccgctt cttcagcctg ccagccgaaa cggtgagaac gagaggccgg
     8521 gttaccaaag gttccgtcca tcgtcatgaa ctgcatcatt ttctcggcaa cacgcgggtc
     8581 caccggcgtg gttgcggagt agtcgagata aatcggtaat ttcattgctc tataaactcc
     8641 gtacatcact caatgcaagg aatcaggcta ccggctggat gtacgaccgt gtttacggag
     8701 tatttagcac tccggcctga ttctgaattc tttttattaa gcgcgtaact taacgtcgat
     8761 cgcgtcttgt gtgcgggtgc gtggcgcgtc gtgagtatgc tgacgaccag acacatccag
     8821 cacttcctgg ttattaacca gttcgcctaa agtaatgttg ttgagaaaac cggtgagacg
     8881 gtcgctcaaa tcacgccaca gcgcgtgggt caggcattta tcgccgccct ggcagccgcc
     8941 tttaccctga caacgggtgg catctacaga ttcgtcaacg gcgctaatca cttcgccaac
     9001 ggcgatgctg ctggcatctt tgcctaacag ataaccaccg cctggtccac gtacgctgga
     9061 aaccagacca tttttacgca gacgggaaaa cagttgttcc agataagaaa gggaaattcc
     9121 ctgacgttcg gaaatatcag ccaacggtac cgggcccgct tcagagttga gcgcaacgtc
     9181 aagcattgcg gtcacggcat agcgcccttt agatgtcagt ctcatgtctt acttcacctc
     9241 aaactcgccc ctgcccgggg ttttttattg taaaagtggg ggtattgcat agcagggtca
     9301 agtctgacat tcccgagtaa attggtcaac tatttacttg actgatttag tcgggtattt
     9361 aaccttcagt gccatttttt tatcgtggcg tttgtacctg ttgtcggatg cggtgtgaac
     9421 accttatccg acctacgatt ctgtacattg taggcctgat aagacgcgtt aagcgtcgca
     9481 tcaggcaacc actgttattc ggccttgtta cctttattct gctgctcaat agaagccaga
     9541 atcccgcgca ggatattcaa ttcctggctt tccgggcgcg cacgggtaaa cagacggcgc
     9601 aatttattca tcacctgccc cggatggttt tcacggataa aaccggttgc cagcagcgtt
     9661 tgttccagat gaccataaaa acgctccaga tcatcgacca gcggatacgg cgtctcttca
     9721 tgctcgacct gctcgccatt ttcctgagtt gccagccagg ccatacgcac ttcataggca
     9781 ataacctgaa ccgccattgc caggttcagc gaactgtatt ccgggtttgc cgcaatcgcg
     9841 acatgataat ggcatttctg caactcttca ttggtcaagc cgacgcgctc acgaccaaac
     9901 accagcgcca ccggggtatt tgccgcttca gcgacgcttt tcagaccgca ttcgcgcggg
     9961 tcgagcatcg gccacggcag cgtgcgggag cgtgcgctgg tgcccaccac caggctacaa
    10021 ccggctaacg cttcgtcgag cgtatcgacg atgtgagcat taccaatcac atcgctggcc
    10081 cctgcagcca gggcaatcgc ctgggagtcg ggtttcacca gtggattaac cagccacaga
    10141 ttggttaatc ccattgtttt catggcacgg gcaacagaac ccatattgcc ggtgtgtgac
    10201 gtctccacca gcacaattcg aatattttgc agcattgtct ttcttcatct aaagattatt
    10261 cacgcatctt atcataaaac gaagacagat gccgatctcg ctgctatact ctgcgccgtt
    10321 ttcccgttct ttaacatcca gtgagagaga ccgatgcatc cgatgctgaa catcgccgtg
    10381 cgcgcagcgc gcaaggcggg taatttaatt gccaaaaact atgaaacccc ggacgctgta
    10441 gaagcgagcc agaaaggcag taacgatttc gtgaccaacg tagataaagc tgccgaagcg
    10501 gtgattatcg acacgattcg taaatcttac ccacagcaca ccatcatcac cgaagaaagc
    10561 ggtgaacttg aaggtactga tcaggatgtt caatgggtta tcgatccact ggatggtact
    10621 accaacttta tcaaacgtct gccgcacttc gcggtatcta tcgccgttcg tatcaaaggc
    10681 cgcaccgaag ttgctgtggt atacgatcct atgcgtaacg aactgttcac cgccactcgc
    10741 ggtcaaggcg cacagctgaa cggctaccgt ctgcgcggta gcaccgctcg cgatctcgac
    10801 ggtaccattc tggcgaccgg cttcccgttc aaagcaaaac agtacgccac tacctacatc
    10861 aacatcgtcg gcaagctgtt caacgaatgt gcagacttcc gtcgtaccgg ttctgcggcg
    10921 ctggatctgg cttacgtcgc tgcgggtcgt gttgacggtt tctttgaaat cggtctgcgt
    10981 ccgtgggatt tcgcggcagg cgagctgctg gttcgtgaag cgggcggcat cgtcagcgac
    11041 ttcaccggtg gtcataacta tatgctgacc ggtaacatcg ttgctggtaa cccgcgtgtt
    11101 gtgaaagcca tactggcgaa catgcgtgac gagttaagcg acgctctgaa gcattaatga
    11161 ctcaggcggg tgatatcact cacccgccct cgcctttcag gcgctattcc gaaatacttc
    11221 ctcaccgctt tactttcttt cgtcactctc ccaccatctt tccccgatta atggatgata
    11281 aagaagtcgc aaggaaaggt tatggcactg ccagtgaaca aacgcgttcc caaaattctg
    11341 tttattctct ttgttgttgc cttctgcgtt tatttagtgc cgcgcgttgc catcaacttc
    11401 ttctattatc ctgacgataa aatttacggt cccgacccct ggtcggcgga atccgtcgaa
    11461 tttacggcta aggacggtac tcgtctgcaa ggctggttta ttccttcttc gacgggccct
    11521 gctgacaacg ccatcgcaac catcattcat gctcacggca atgccggaaa tatgtccgcc
    11581 cactggccgc tggtcagttg gttacctgag cgtaatttca acgtttttat gtttgattat
    11641 cgcgggtttg gtaaatcaaa aggcactccg tcccaggccg gattgctgga cgatacgcaa
    11701 agcgccatca atgtggtgcg ccatcgcagt gatgtaaacc cacaacagct ggtgctgttc
    11761 gggcagagta ttggcggggc gaatattctt gctgttattg gtcagggtga tcgtgaaggc
    11821 atacgtgcgg tgatcctcga ctccacattt gcctcttatg caaccatcgc caaccagatg
    11881 atccccggca gtggctactt acttgatgag agttacagcg gcgaaaatta catcgccagc
    11941 gtcagcccga tcccgctttt actcattcac ggtaaagccg atcacgttat cccatggcag
    12001 cacagcgaaa agttgtatag cctggcaaaa gagccaaaac ggttgatcct aatcccggat
    12061 ggcgaacaca ttgatgcttt ttccgatcgt cacggcaatg tttatcgcga acagatggtg
    12121 gactttatcc ttagcgcgtt gaatccgcag aactaaccca tgatcgctag cacgataatc
    12181 attcacaaaa ccaccttaag acatgctaat ccactggtca gaacagttta aaatgagaaa
    12241 aattctgtga cgcttgccaa catttctgat gattagcatt cccttcgcca tttccctgag
    12301 caaactttag ctattcttat caattatgct tatgggagat ctacagatga tgcctacgct
    12361 tgctccacca tctgtccttt cggctcccca gcgccgctgc cagatcttgc tgacgctctt
    12421 tcagccgggg ttgaccgcca ccatggcaac cttcagcgag cttaatggtg tggatgatga
    12481 tattgccagt cttgatatca gcgaaacagg acgggagatc ctgcgctatc atcaactcac
    12541 actgacgact ggttatgacg gtagctaccg ggttgaaggt acagtgctta accaacgttt
    12601 gtgtttattt cactggctac gacgtggttt ccgtctgtgt ccgtcattta ttaccagcca
    12661 attcaccccc gccctgaaga gtgaactgaa gcggcgcgga attgcgcgta acttttacga
    12721 cgataccaat ctacaagcgt tagtgaatct ctgctcccga cggctgcaaa aacgctttga
    12781 atcgcgcgat attcatttcc tgtgtctgta tctgcaatat tgtttgctgc agcaccacgc
    12841 tggaattacg cctcagttta atccgctcca acgtcgctgg gcggaatcct gccttgaatt
    12901 tcaggtagcg caggaaattg gacgccactg gcagcgtcgg gcgctccagc atgtaccgcc
    12961 tgatgagcca ctgtttatgg cattactttt ttccatgttg cgggttcccg atccactgcg
    13021 ggatgcgcat cagcgggaca gacaattgcg tcagtctatc aaacgtctgg taaaccattt
    13081 tcgtgagctg ggaaatgttc gtttttatga tgaacagggg ttatgtgatc agctttatat
    13141 ccacctcgcc caggcgttaa atcgcagttt gtttgccatc ggtattgata ataccctgcc
    13201 ggaagagttc gccagactgt atccacgcct ggtacgcacc acccgcgcgg cgctggccgg
    13261 atttgaaagt gaatacggcg tccatctttc tgatgaggaa agtggtctgg tcgcggtgat
    13321 tttcggtgcc tggctaatgc aggaaaacga tctgcatgaa aaacagatta ttctactgac
    13381 cgggaatgat agcgagcgag aagcgcagat tgagcagcag ttacgcgaac taacgttact
    13441 gccgctcaac attaagcata tgtcggtaaa ggcatttttg cagacaggcg ctccgcgtgg
    13501 cgcggcactg attattgcgc cttataccat gccgttaccg ctcttttcac caccgctgat
    13561 ctatacggac ctgacgctga caacacatca acaggagcag atccgcaaaa tgctcgaatc
    13621 agcatgaggg aacaactttc gggcgcaaaa acattgccgg aagcgccacc agcgccatta
    13681 cccagaacac gccgtggccc agatattgat acaggaaacc ggcgaaaacg gtcatgatag
    13741 cgatactgcc gcccatcgcg acggcagagt aaaccgcctg taaacggatg acttcgctaa
    13801 cctggcgagc agcaatataa cgcatggcgg ccaggtggca gaccgtgaag gtgccgcaat
    13861 gcagaatttg caccactatc aaccacggca acgccgtagt tgctcccata atgccccagc
    13921 gcactacgcc gcaaatcgcc gagatcaaca gcatatcgcg agcactacaa cggcggaaaa
    13981 gtttattact cagcgcaaag ataatgactt ccgccaccac gcccagcgac cacaaatacc
    14041 ccaccgccga ggccgagtag ccagctgcct gccagtaaat ggcgctaaaa ccgtaatagg
    14101 ccgcatgtgc cccctgcaat aaacaaacgc aggccagaaa gcgccagttc tggcgaacca
    14161 gcgccaacca cgcagaccaa ccggtgctct cctgctggcg gcttgcccct tgtggctgaa
    14221 tcgtcggacg gatgagaaag ccgagcagca tggatgccac gcccaacgtc aacagcacga
    14281 ggatcacccg ataatcaaac atagtgacca gtttgcccgt cagcgccgag ccaatgacaa
    14341 acgccaccga gccccacagt cgcactttgc cgtaatcaag cgggaactgc ttttgccacg
    14401 tattcgccag tgcatcggtc aacggtacca gcggtgagaa aaagaggtta aagccaatca
    14461 tcaccagcat cagccacgct acgtgcgccc ccgcccagaa ggcgacagca aagagaagtg
    14521 gcagcagtgc cagcacgcgc aaggcggaaa tcaggcggga aggatcgctg acgcggggcg
    14581 cgatgagcaa actcccgaga aaacgggcaa ccagacctgc ccccaataac aggccgatgg
    14641 tttctggcgt taaaccaatc cctttaagcc agacgctcca gaaaggtaaa aaaatgccgt
    14701 aactaaaaaa gtatgtgaaa tagccgagcg ccaaccagcg cgtggattgc aaaaccatga
    14761 gtccctcccg tcaggaggcg ttagtctggg gttaatcgaa tgttggtgca agttgaaaaa
    14821 taagaatatt cgagcctgct tcccgatgtc tgtaacgcca gttcacaagc actaaaaaac
    14881 tgcgatccgc gacgcatgtt gcaaaaataa tgcgtaatta tgccgttacg cttgccaaac
    14941 gttcctgcac aatggcgatg aaatcgcgta atgccagctt catctcccct ttcttccacg
    15001 ccatcagtaa agcaatggaa ggcacatttc cggcaatggg acgaaaaaca acctgcccgg
    15061 tattaaaatt attcatataa ccgggtatca aagtgacgcc cagccccatg cccaccagat
    15121 tcatggtcac cagaatattc gttgccacct ggacgatatt tggctggctg ttttcttgcg
    15181 caaaccacgc tttaacgatc ggcgcaagcg aaccggaata cgccggatcg gtactgacga
    15241 aattcacgcc atccagttgc gcggcggtga tctctttttc atgtgctaaa gggtgatcaa
    15301 ccggtaacac caccactaat ggttcgtcaa aaagctccag ataatcaatc tccgggctat
    15361 aaacaggatg gcgcattaag ccgacgtcga gttcaccacg gcgaattttt tcctcctgtt
    15421 gcgtggtgat taaactcacc agctcaatca aggtgtctgg ctgtctgaga cgaaacatcg
    15481 gtaatacttt tggcaataaa ttcacttccg ccgatggcac aaagccaatg gttaattgtc
    15541 tgtcttcctg aacaattttc cgcgcccgta atttggcatt ttccgcttgt tcgaggattg
    15601 ccagcgcgtc ctggagaaaa cattctcccg ccgccgtcaa cgcgactttg cgcttatccc
    15661 tcaccagtaa cggaacaccg acacagtttt caagatcgcg gatctggctg cttaacgaag
    15721 gctgtgaggt atgcagtttt tccgccgcac gggtaaagtt cagtgcctgc gccactgcga
    15781 cgaaatagcg taaatgccgt agttccatca ccttcccctt gttatcgaaa aaacgtctca
    15841 gctggtagaa attaactatt tcacatatta gcaaccaacc agcaacatcc ttatggcaca
    15901 aaaatagaag gtcaatacat cttatctttc aggattaaaa aatatgacca caccctcaga
    15961 tttgaacatt taccaactga ttgacaccca aaacggtcgg gtcactccgc gtatttatac
    16021 cgacccggac atttaccaac tggagcttga gcgtattttc ggtcgttgct ggctatttct
    16081 cgcccacgaa agccagatcc caaaacccgg tgatttcttt aacacctaca tgggagaaga
    16141 tgcagttgtc gtggtgcgcc agaaagacgg cagcatcaag gcgtttctca accaatgccg
    16201 ccaccgggcc atgcgtgtga gttatgcaga ttgtggcaac agtcgcgcct ttacctgccc
    16261 gtatcacggc tggtcttatg gcattaacgg cgagttgatc gatgtaccgc tggaacctcg
    16321 cgcctaccca caagggttgt gtaaatccca ctgggggcta aacgaagttc cttgtgtgga
    16381 gagttataaa ggactgattt ttggcaactg ggataccagc gcaccgggcc tgcatgatta
    16441 cctgggtgac attgcctggt atctggatgg catgctggat cgtcgcgaag gcggcaccga
    16501 aattgtcggc ggcgtacaaa agtgggtgat caactgtaac tggaaattcc cggcagagca
    16561 gttcgccagt gaccagtatc atgctctgtt cagccatgct tctgccgttc aggtattagg
    16621 ggcgaaagat gatggcagcg ataagcgcct cggtgatgga caaaccgccc gcccggtgtg
    16681 ggaaaccgcc aaagatgcgc tgcaatttgg tcaggacggt cacggtagcg gtttcttctt
    16741 tactgaaaaa ccggatgcta atgtctgggt cgatggcgca gtttcaagct attaccgcga
    16801 aacctatgcc gaagcagaac aacgtttagg tgaagttcgc gccctgcgcc tggcgggtca
    16861 taacaatata ttccccacgc tttcatggct aaacggcact gccacactcc gcgtctggca
    16921 tccgcgcggc cctgatcaag ttgaagtgtg ggcgttctgt attactgaca aagccgcctc
    16981 cgatgaagtt aaagccgctt ttgaaaacag cgccactcgt gcttttggtc ctgctggttt
    17041 tctcgagcag gatgactcgg agaactggtg tgaaatccag aaattgctta aaggccaccg
    17101 cgcccgcaac agcaaactgt gtctggaaat ggggcttggt caggaaaagc gccgcgacga
    17161 cggcattcct ggcattacta actatatctt ttcagaaact gccgctcgtg gaatgtacca
    17221 acgctgggcc gatcttctga gtagcgaaag ctggcaggaa gtgctcgata aaaccgccgc
    17281 ttaccagcag gaggtgatga aatgagtgcg caagtttcac tggagttaca tcaccgcatt
    17341 agccagtttc tctttcacga agccagtctg ctggatgact ggaaatttcg tgactggttg
    17401 gcacagctcg acgaagagat tcgttacacc atgcgcacca cggttaacgc gcaaacacgc
    17461 gaccgtcgca aaggcgtcca gccgccgacg acctggattt tcaatgacac caaagaccag
    17521 ctggagcggc gaatcgcccg tctggaaacg ggcatggcct gggcagaaga gccgccgtca
    17581 cgcactcgtc acttaatcag caactgccag gtaagcgaaa ccgacatccc aaacgtattt
    17641 gccgtgaggg tgaattatct actttatcgg gcacaaaaag agcgcgatga aacattctat
    17701 gttggaacgc gtttcgacaa agttcgccgt ctggaagatg acaactggcg cttgctggaa
    17761 cgggatatcg tcctggatca agcggtgatc acttcccata acctgagtgt actgttctga
    17821 tgaatcgaat ttatgcgtgt cccgttgcgg atgtgccgga gggtgaggct ctccggatag
    17881 atacctcgcc cgtcatcgcc ttgttcaacg tgggcggcga gttttatgcc attaacgatc
    17941 gttgcagcca tggtaatgcg tcgatgtcag aagggtatct ggaagatgac gccacggtgg
    18001 agtgcccgct acacgccgcc agtttttgcc tgaaaacggg gaaagcgtta tgcctgcccg
    18061 ccaccgatcc gctcaccact tatccagtac acgttgaagg tggtgacatt ttcatcgact
    18121 taccggaggc gcagccatga gcgatctgca taacgagtcc atttttatta ccggcggcgg
    18181 atcgggatta gggctggcgc tggtcgagcg atttatcgaa gaaggcgcgc aagttgccac
    18241 gctggaactg tcggcggcaa aagtcgccag tctgcgtcag cgatttggcg aacatattct
    18301 ggcggtggaa ggtaacgtga cctgttatgc cgattatcaa cgcgcggtcg atcagatcct
    18361 gactcgttcc ggcaagctgg attgttttat cggcaatgca ggcatctggg atcacaatgc
    18421 ctcactggtt aatactcccg cagagacgct cgaaaccggc ttccacgagc tgtttaacgt
    18481 caacgtactc ggttacctgc tgggcgcaaa agcctgcgct ccggcgttaa tcgccagtga
    18541 aggcagcatg attttcacac tgtcaaatgc cgcctggtat cctggcggcg gtggcccgct
    18601 gtacaccacc agtaaacatg ccgcaaccgg acttattcgc caactggctt atgaactggc
    18661 accgaaagtg cgggtgaatg gcgtcggccc gtgtggtatg gccagcgacc tgcgcggccc
    18721 acaggcgctc gggcaaagtg aaacctcgat aatgcagtct ctgacgccgg agaaaattgc
    18781 cgccatttta ccgctgcaat ttttcccgca accggcggat tttacggggc cgtatgtgat
    18841 gttggcatcg cggcgcaata atcgcgcatt aagcggtgtg atgatcaacg ctgatgcggg
    18901 tttagcgatt cgcggcattc gccacgtagc ggctgggctg gatctttaag gaagcacgat
    18961 gaaagaaaaa acgatcatta ttgtcggtgg cgggcaagcg gcggcaatgg ctgcggcctc
    19021 gctacgccag caagggttca ccggtgagct gcatctgttt tccgatgagc gacatcttcc
    19081 ttatgaacga cctccactct cgaaatccat gttgctggaa gattccccgc agttacagca
    19141 ggtgttaccc gctaactggt ggcaggaaaa caatgttcat ctgcattccg gtgtaaccat
    19201 caaaacgctg ggtcgcgaca cacgagagtt agtgttaacc aacggcgaaa gctggcactg
    19261 ggatcagctt tttatagcaa ccggcgcggc agctcgaccg ctgccgttgc ttgatgcact
    19321 gggagaacgc tgctttaccc tacaccatgc cggtgatgcc gccagactgc gagaagttct
    19381 gcagcccgaa cggtcagtcg tgattgtcgg tgccggaact attggtctgg aactggctgc
    19441 cagcgccacg cagcgcagcg cagcgcagcg cagatgtaag gtgacagtga ttgaactggc
    19501 ggcaaccgtc atgggccgta atgcaccacc gcccgtgcaa cgctatcttt tacagcgcca
    19561 tcagcaggct ggtgtgcgca ttctgctcaa taatgccatt gaacatgtgg tcgatggtga
    19621 aaaagtagaa ctgacgctgc aaagtgggga aacgcttcag gctgatgtgg tgatttacgg
    19681 tattggtatc agcgccaacg agcaactggc tcgcgaggcc aaccttgata ctgccaatgg
    19741 cattgtcatt gatgaggctt gccgcacctg cgatcccgcg atctttgccg gtggcgatgt
    19801 ggcaatcact cgtcttgata atggtgcact acaccgctgc gaaagctggg aaaacgccaa
    19861 taaccaggcg caaattgccg ctgccgcaat gttagggcta ccgctaccgc tactgccgcc
    19921 gccgtggttc tggagcgatc agtacagtga taacttacag tttattggcg atatgcgtgg
    19981 cgatgactgg ctttgtcgtg gcaacccgga aactcagaag gcgatttggt ttaatctgca
    20041 aaacggcgtg cttatcggtg cggtcacgct gaatcagggc cgtgagattc gcccaattcg
    20101 caaatggatc cagagcggca aaacgtttga tgcgaaactg ctgatagatg agaacatcgc
    20161 gcttaaatca ctgtaatcag gataattagc gaatatctca atgcctgggg cgtggcgagg
    20221 tgcaagagtg tgtattacgt ttaaatcaca ttatcttgca aagggattgg ttatgaacac
    20281 actacgttat tttgattttg gagctgcccg ccccgttttg ttattaattg cccgtatcgc
    20341 cgtggtctta attttcatta tttttggttt tcccaaaatg atgggctttg acggtacggt
    20401 ccaatatatg gcctcgttgg gcgcgccaat gccgatgctg gcagcgatta ttgcggtagt
    20461 tatggaagtg cccgccgcga tattaatcgt gcttggcttt ttcacccgtc cgctggcggt
    20521 gctgtttatt ttctacacgc tgggtacggc ggtgattggt caccattact gggatatgac
    20581 cggcgatgcg gttgggccaa atatgattaa tttctggaag aatgtcagta tcgctggcgc
    20641 gttcttgcta ttggcaatta ccggaccggg ggcaatttct ctcgatcggc gttaggaaag
    20701 atgccggatg cggcgtgaac gccttatccg gcattttata aattacagcc actctacccg
    20761 caacgacatc tctgaggttg ttgattcccc tggtgctagc gcaatgagat cacccccttc
    20821 cgggcgatga tgctcatccg gcgcatggct catcggttcc agacagaaga aatcaaacgc
    20881 atatccttta tcaaacgcag ggtcggaaac aaagatgaaa taacacggtg caggtggcgt
    20941 cgtttccatg atgatggcat aaccttcctg cggctgctcg atgcgggcct gaccattcca
    21001 tccggcaaaa ccattgttca cccactgacg cggcaacggc gcgagctggt taaaatccag
    21061 ttcctgcggt agctgctcgc aaaactcacc cgccagccac tgctcccgtt ccagccagta
    21121 accgctcgcc tgcgcctgaa tccgcgtttg tggtgacaac ggaaaataag gatgccagcc
    21181 ggtaccaaat ggcagcgtct ctgccccttg attggtgaca gagagcgtca ccgtcagcgt
    21241 atccgccgtt aaatgaaacg cctgacttac ccgatagtga tagacaccgc tgcgatgttc
    21301 atacaccaga cacaaactat catcgctgtg cgagacacat tgccactcgc ccagccagcc
    21361 atcgccgtgt agatagtgcg catcccactc aacattcggt tgcagttgat actcacgccc
    21421 ctgccagaca aaacggttgc cgctcacccg gtttgcaaat ggcaccagcg gaaaacatga
    21481 ggcatcggtt gccacaccgc tttttttacc aggacgtaag agcggcgtcg tatcgcgcca
    21541 gaagccttcg attacgccgc cctggtcaga aacgtccagc tttagcgacc cgtgggataa
    21601 ggtatagatg gtcatccgct aactccttaa tccgggaagt taatcacaac tttcccgcat
    21661 ttgccgctcg ccatcagcac ataggcatca cctgcctgtt ctagcgagaa gcgatgggta
    21721 atggcgttac gcggccacag cttccagtca gtcaaatcgt gggcgcattt ttccatatgg
    21781 aacagactgg tcacccagga gccgataatc cgccgttgat ggtgcatcag atcagcgctg
    21841 acctcgaatt ccacttttcc ggtttcacca atgtaaacca cccgccccca gtcagcagtg
    21901 gattgcagtg ccagcaagcg acctgcggca ttaccggaac aatcgagcgc aacatccgcg
    21961 ccaccgtggg taagttcagc aattatctgc ggcagacctt cggtggttgc taaatagccg
    22021 tgatccatcc cccctaactg ttttgccatc gccagacgtt ccggcagcat atcaacgccg
    22081 atgatccgtt ttgcaccgcg acctttcgcc agcatcatcg ccatcatgcc gaccggcccc
    22141 agaccgacca ccagcacgtt atcgctgccg gaaacttcgc cgcgcaaaat cccttcatag
    22201 gccgtgccaa cgccgcaact gataaacgca ccatcttcgt agctcagcgc atccggcagg
    22261 aggatcagat ctttttcttc cgccagcaga tattcggcat gaccgccgtc acgctgccag
    22321 ccgtaagccg cttttccttt gccagtacaa gagataggaa agccgcggcg gcagttcggg
    22381 caaaaaccac agccagaaat gtgatacacc agcacgcggt cgccctcttt aaaatggcgg
    22441 cagccttgcc ccatcgccac aatctgcccg cacggttcat gaccgttgat aaatccctgg
    22501 tataacggtt tatcgggtgc cgccgccgtg gcacggtgtt gatgatagat atagtggaca
    22561 tcgcttccgc aaatcccgga ggatttcatt ttgatcagta cctggttaat ccccggcgtc
    22621 ggcaccgcaa cttcccgcag atcgacggtt gaatttccta gtaaataagc agccagcatc
    22681 gttttcataa atccctcatt aacaatacga ttaattttca tccctgcagc acccgcgcca
    22741 ggggcgttaa ggtttagcgt ttcgctttac tgcttcgctg ggtcagcaag atattcgcca
    22801 gcaccgccac cacgatgatg acgccgcgta ccacctgctg gaaaaaggag ttaataccga
    22861 gcagcaccag accgttaccg attagcgtaa tcaccagcac accaagcaat gtaccgaaca
    22921 gggaaccgcg accgccggaa agtgccgtac cgccgaccac gaccgcggcg atgacgtcaa
    22981 actccagacc gtttgcggca cctgcgttac cggaaccgag gcgtgccgcc aacaaaatgc
    23041 cggtcaccgc cgctaataat cccgaaaggg taaagataag aatgcgcact cgacgaacgt
    23101 taataccgca caactgcgcc gccgtggcat taccgccaac ggcaaaaacc gagcgcccga
    23161 aggcggtttt tcggctaatg aacacaaaca gcgcaaacaa caccatcatg atcagcgcgg
    23221 atactggcac accgagaaat tgtccgccca gccagtccag cacctcgttt tcgtcaatcg
    23281 gcactggcag tgcgttggtc ataaacagcc ccattccgcg cagagcgctc cacagcccca
    23341 gtgtggcaac gaaacttggt acgttaaaca cgccgcgcag caccccggcg agcgttccca
    23401 tcagcgcgcc taacagcaac accagcagac aagcaatcgc caacggaact tcaaattgca
    23461 gcaaaaatgc caggcacacc gagacaaaag ccaccatcgg cccaacgctg acatcaattt
    23521 caccggagat aataatcagc gtcatcgccc aggcggcaat cccaatggtg gcggcatcgc
    23581 gcagcacgtt catctggtta ttcaatgaga taaagccagg cgcgttcagg gagaagacca
    23641 gataaagaat ggcaatcacc accagcaaac cgatctcatt aatatggcga ctgacaaatt
    23701 gtttgagcga gacgctcttg ccctgcggca atggtaatga cgaagcatac atcttcaggt
    23761 tcctcggtaa aattcatcag tgcacagaca gaatggcgga catcagctca tccacattga
    23821 ccggcgagtg aaactcctgc gagaacgtgc cgtgctgtaa taacaggatg cggtcacaca
    23881 ccagcggcaa ttcctccact tcactggaga taaacaccac gctttttcct tcggcagcca
    23941 gctcacggac aatacggtaa atctgctgtt tggcttcgat atcgacgccg cgcgttggct
    24001 cgtcgagcaa caaaatctgg ctggcggcat agacccaacg accgatcacc actttttgct
    24061 gattgccacc agaaagcgtg ccgatgggtg tttcgctact ggcagccttg accgtcatcc
    24121 gctgcatcac ctcttcggtc aggcggcgga tggtggacca ttgcagcaca ccgttggtgc
    24181 tgattttttg ccgattggtc agcactgtat tttcgtcaac gcctagccag ggaatgatcc
    24241 ccgcttcttt gcggttttct ggcgtatagc caatgccgcg tttcagcatg tcgccgtaat
    24301 cggggcgcat gattttctcg ccgttgataa caatttcgcc ctgttcatac gtctccagcc
    24361 caacaatcgc cttcagcaat tcactgcgcc ctgcccccag caggccagca atgccaagca
    24421 cttcgccacg acgtagcgta aagctgatat cctccagctt gggcttatgg cgtaacgcac
    24481 ggacttccag tacggcctga tccacaattt cctgaggggc aaccggcgca atatcaacgt
    24541 gatcacgccc tagcattagc gacacaatat gatgcgtgga ggtgttttcg agcatcacat
    24601 cgcccgccac ctgaccatcg cgcataacgg tggcatagga ggcaatgcgg cggatttctt
    24661 ccatccggtg gctgacataa atcaccgcca cgcccagtgc cgacatcttt ttcaccgcac
    24721 tgatcaccag ttcaacttct gcactggcaa gcgagctggt gggttcatca agaatgacca
    24781 cgcgcggctc gcccttcatc acccgtgcaa tttccaccag ttgcttttgc gccgggctta
    24841 gcgttgaaac aagttgttca ggactaacgt caacgcccag cgcctgtaag caacgttggg
    24901 catcctgcgc catttgcagg taatcaatca tgccgttgcg gcgaggccat tgaccaaggc
    24961 agaggttttc tgccaccgtc agcccttcca ccagacttaa ttcctgataa accgcacgaa
    25021 cccccagttc agcggcacgg cgagtcaacg tagcttcgtc accttccagt cgcgtctcgc
    25081 caatccagat atcaccgcta tccgggcgtt cgctgccggt aagcattcga atgagagtcg
    25141 atttgcccgc gccgttttta cctaacagcg cacgaacttc gcctttattg agcgtgaagt
    25201 taacgttatc cagcgcaacg acgccggggt aacgcttatt tcctgccacc acttttgcta
    25261 ccgggactgc ctctgttgcc gtgaacatag ggacctctgc gaatcagcga ttagggcaga
    25321 ccatcaacgt gcgttgccag ccactgttta ccatcttccg ttttggtata gagatcgatt
    25381 ggcacctgaa tcactttttc accgtcggct tgtttattga taaccttcaa tgtttgcgcg
    25441 aaaacagcat tgcccatttt cttaccggaa atatccacta ccgctttcag cacctgattg
    25501 ttttccagct cctgagcaat ttcggttgtc atatccgaac cgaaaacagc aatttttccg
    25561 gcctgatttt gattacgtac cgcttttacc gcgccgagtg tcgcaccgcc cgactccccc
    25621 ataatggcgt tgagatccgg cgtggagata atcagttttt caccaacgga aatcgcttta
    25681 tctaaaacag tcccttcctg gttagcgaca atttgcgcgc cgggaacgcg ggattttaat
    25741 acttcttcaa atcctttacg tcgctgcaca caaacttcaa aggcttcgca attgatgacg
    25801 gcaattttcg gctggtcaat tttattggca ataaaataat cggcggcagc gttacccagt
    25861 ttttaccaaa ttccagtgga tcgccgacca gatacgccga gacatattta tcgacaccct
    25921 tttgattaat acaggtgttg tagcaaatca ccggaatacc cgcttcactg gcgcgacgaa
    25981 cggtacggct actgccattt tcagacactg ccgataaaat aatggcatcg acattacgcg
    26041 ccacgagggt atcaacaaag gtactttctt tcgaaatatc cccctgggcg ttagtttcaa
    26101 ttaactgtac ctgtactgaa gaatcttttg ccgcatcctg aacgccctga cgcactccag
    26161 cgtaatatcc ctgggtatca aggtatattg caccaatggt catttcttta tccgcagccc
    26221 tggcaaatag tgcgctacct aatagcgtag ccattaataa tagattacgg gttgttctca
    26281 tttttttagg catagtgcct cctgtagggt ttttattaac aacggcttat tctaattatt
    26341 ttgtgatgag cggcagcgcg tgccgctcaa atattacagt taatagggtt tagtgaacgt
    26401 agttaaaaat aaaaggcatc acagtggtga ataatgccgc tttcggccag gccggattaa
    26461 tcgccgtcaa ttcagcgcga gcagactcat attgcgcaac ctccccgagt cccgcggacg
    26521 ccagcatcgc caccatcagg catttttctt tatgctgctg ttgtaaatcg ccatacagcg
    26581 ataacaggtc aggttgtgaa acagcaaaga aatccgcctc aatactggtt ttcgccatct
    26641 cttgcgccca ctgtttcatt tcactaaaca gttgctgtgc ggtttgctgt tcacccagca
    26701 gtcgcagcgc cattccttgc cagaagagat aatcaaccgg ctgatcgttg taataactgt
    26761 ggatgttaat ggtgcgatcg ccggtcgccg ccagacgtaa acaacgcatc gcttcagttt
    26821 catcgccctg cgcgttggcg catatcgcct gccagaacca gatgtcgtta tcagtttgcc
    26881 ccggtaaacg gccttcgctt aaattctccg gataatacag cgcggcatga agcagttcgc
    26941 tggcctgctg cggctctctg gcatcaagat gctgccaggc gcgtaataac tggttgagga
    27001 taaactgact ggtgaccttc ccttccccgc cttcccacgg gtggaattta cgcgtggcga
    27061 gaatgtccgc cgctttgtct gcctgacccg tgagatgcca caaattgagc agttctgcgg
    27121 tcatgtcgtc gcgtttcagc gcaatttcca gattattttc cagacgcgcc agtcgtttct
    27181 ccggtgtggc tccacttaac ttatccagca aatcccgttc gaaaagcaga cgtgcatcct
    27241 gcggcgcaag ctgataagca ttatcaagat aacgcgcggc cagctcataa tcgtgtttct
    27301 tattccacgc atggatcgct aacccgcgcc agccgtcggc aaactccggc gacatctcta
    27361 cgcaacgttg ccataaggca atggctttgt tgtagctacg tttgttgtag tagaagcagg
    27421 ccagtaaatg gcgagcaaac caacactctt caatactctc cagcgccgcc acttcttcca
    27481 gcgtattcgg gaaacggaca aactgcggga agacatcaat ggcttttgcg accagttcgc
    27541 cacgttcggc tttcggcagc aagctggctt gcaggtaaag cggcagcgtg cgctggcagt
    27601 ccagtgcgtt cagcatctct gccgccaggg tgggcattcc ccagttaatc agttgcccgg
    27661 cggtcatcag agcgttaacg tcgcgtccct gacacagacc gcgccactgc gcgagggctg
    27721 attcgctacg accatcgaac cagttcagcc accacagagt ggcgttcagc ggataatcac
    27781 gcagcagttt ctcgcgctgc aaacgcgcgt tgtcctgacg accacttaac accagcagca
    27841 gattatgcaa gcaaagcact tcctgattgg ttgggcaggc acgaagactt tgttggcaaa
    27901 aatccagacc cgcgtcgaag ttatcattac gcgccgccag tcgtgccaga ccataatagc
    27961 cgcctgcttt actattgccg ctccagaccg cacgccagaa atcctcttcg gcttgttgat
    28021 attgtccctg acgttcgtaa gcactggcgc gaatcaaact tgcttgtcca cactgcggtt
    28081 ttttgttcag cgcatgtgca cgtttcagcg cctgactggc atacgccacc gcttgcggga
    28141 aatctgcgcg gttatattcc agcatcgcca gcgccaggtt acagcgataa tccagcgggt
    28201 ccagcgccac gccgcgcagg tagtaatcga acggtgaacg gctggcgtga tgatattgct
    28261 ccagatgctg accgataaac caggcttcat ctgtactggt aatgtcttgt gctgccagtg
    28321 gcgctttggc gacgtccggc aacggcaact cctgcggctg atgttcctga taactcagta
    28381 caatattgcc gtcggcatcg gagagttcaa tggtcagtct gtcaggattg ataccgtgca
    28441 acacgccctg gatggcggtc gctggtgtca gggccacggc atcatcgagc aacgcgttgc
    28501 atttgccgat ttcgcggatc gccagacggt atccgttcaa cggagagatg gcatacagcc
    28561 cccactcaat cccccgctca ctacgctgga gttttatcac cgcatcgcgg gaggcatttt
    28621 gcaccatgcc cagagaatga taaggcagga aatactgctc gaaacgcttc tcttcgtaag
    28681 catcaagcca ggtaaaatca ggctggttat cggcaaaaat accggtcatc agttcgatat
    28741 acgggccgtt attgtcggtc agactcttat cccacgcctg gccaaattca ctgtgtcccc
    28801 aactccactg ttttttacct ggcgcaatat ggtggttggc aacgtgcagc aaaccgccat
    28861 cttcatcgtg acaccacgcg ccgacaaaat cgtactgcga tttttcggcc atatatgagg
    28921 ttggaacagg cacattttta tagcgagaaa tgtccactcc ggcggagtag tccactttgt
    28981 agtaagtgcc ggtggcgatg gggaaagcgg agacggcccg tttgccgtga tcaaacaccg
    29041 cagtcacatc cggtgggaaa acgctctgat gcccttcacc ccctttcact gccgggttga
    29101 cccaccacaa gaaatgacgc ggcgtggcgt tgccgttata gacgcggctg gcgatttcca
    29161 gtgccgcccg gtcagggcgc agggtgaaac ctgtcatcac ctgtaaacca tgcatcggct
    29221 ccgtttcgcc gacccacacc gtctgtgcac cgtcttcatg ggcttcgagg gtgaaatcaa
    29281 cgggcataaa ggtggtcggg cgatggtgtt gcggccagtt aaactcaatc ccgccggaga
    29341 tccacggtcc cagcatcccc accagcgcag gtttaatgac ttcattgtga tagacaaaat
    29401 cgcgctgttt cactttatcc catgcgcgat gcacccgacc gcccagttcc ggcaggatca
    29461 tcactttgat gtagtcgttt tccagccaca ccgcctgcta ggatttcagg gttttctgct
    29521 cgctcagcgt atcggtcacg ccgtagggat aaaccgcgcc ggacgatccc tgataaacgc
    29581 gattttccag gaacatggga tgtatatcct gcggcccggt ttcataggtc gggatctcaa
    29641 cgcgctcttg ccacactttt actggagtca tggtgccctc aatattaaca agacatactg
    29701 aattaaaaga tttgtggcag tgtattgaac aatctggcaa tgttttcgcg gaataatcac
    29761 gcaattaact aaacaaggtt tagtgaagat gagagcctgc attaacaatc aacagattcg
    29821 ccaccataac aaatgcgtga ttctggaact gctgtaccgg caaaagcgcg ccaataaatc
    29881 aacgctggcc cggctggcgc aaatttcgat tccggcagtc agtaatattt tgcaggaact
    29941 ggaaagcgaa aaacgggtgg tgaatattga cgatgaaagc cagacgcgcg ggcatagtag
    30001 cggtacatgg ctgattgcgc cggaaggtga ctggacgctg tgcctgaacg tgacgcccac
    30061 cagtattgag tgtcaggttg ctaatgcttg tttaagtccg aaaggcgaat ttgagtattt
    30121 acagattgat gtaccgacgc cgcaggcgct gctgtccgaa atcgaaaaat gctggcatcg
    30181 ccaccgtaaa ttgtggccgg accgcaccat caatctggcg ctggcaatcc acgggcaggt
    30241 tgatccggtg accggcgtat cgcaaaccat gccgcaagcg ccgtggacaa cgccagttga
    30301 ggtgaagtat ctgctggaag agaagctggg cattcgggtg ctggtcgata atgactgcgt
    30361 gatgctggcg ctggcggaga aatggcaaaa taattcgcag gaacgggatt tctgcgtgat
    30421 caacgttgat tacggcattg gctcgtcgtt cgtgattaac gagcaaattt atcgcggcag
    30481 tttgtatggt agcggacaga ttggtcacac catcgttaat ccggatggag tcgtctgcga
    30541 ctgtggacgt tacggcagcc tggaaaccgt cgcctcgtta agtgcattaa aaaaacaggc
    30601 gcgggtatgg ctaaaatcac aaccggttaa tactcagctt gagcctgaaa aactgacaac
    30661 cgcgcagtta atcgctgcct ggcagagtgg agaaccgtgg atcaccagct gggttgatcg
    30721 ctctgccaat gccattggtt tgagtctgta taacttcctc aacatcctca atattaatca
    30781 gatttggttg tacggtcgca gttgtgcctt tggtgagaac tggcttaata ctattattcg
    30841 ccagacagga tttaacccgt tcgaccgcga cgaaggaccg agcgtgaaag cgacgcaaat
    30901 tggcttcggg caattaagcc gcgcacaaca ggtgctggga attggctatt tgtatgttga
    30961 ggcgcagtta cgacagattt gatggcgcga taacgtagaa aggcttcccg aaggaagcct
    31021 tgatgatcat aaacgaaaaa ttgcctgatg cgctacgctt atcaggccta cacggagatt
    31081 gcaatatatt gaatttgcaa gattttgtag gcctgagaag gtgttcatgc cgcatccaac
    31141 atgaacaacg agcacattgt cagcaaatca ccgtttcgct tatgcgtaaa ccgggtaacg
    31201 tgcgcagatg tcgagaactt tacctttgat gcgctcgata acggcttcat cattgatgct
    31261 gtccagcacg tcacacatcc agccagccag ttctttcgct tcggcttctt taaagccgcg
    31321 acgggtaatc gccggagtac ctacacgaat accggaggtc acaaacgggc tcttcggatc
    31381 gttcggtacg ctgtttttgt tgacggtgat gttagcacgg cccagagcgg cgtctgcttc
    31441 tttaccggtc aggtttttat caaccagatc aaccaggaac aggtggttat cagtgccgcc
    31501 ggaaaccact ttgtagccgc gctcgaggaa cacttctacc atcgctttag cgtttttcgc
    31561 gacctgctgc tggtaagttt tgaactcagg ctccatcgct tctttcagag caaccgcttt
    31621 accggcgatt acgtgcatca acggaccgcc ctgaccacca gggaaaacgg cagagttcag
    31681 ttttttgtac agctcttcgc taccaccttt cgccaagatc aggccgccgc gcggacccgc
    31741 cagggttttg tgagtggtgg tagtaacaac gtgagcatga ggaaccgggt tcgggtagac
    31801 gccagcagca accaggcccg caacgtgcgc catatcaacg aacagataag caccgatgct
    31861 gtcagcgatt tcacgcattt tcgcccagtc aaccacgccg gaatatgcag agaaaccacc
    31921 gataatcatt ttcggcttgt gttctttggc ttgtttttcc agatcggcgt aatcgatatg
    31981 accggtagca tcgataccgt aaggaacgat gttgtacagt ttaccggaga agttaaccgg
    32041 agaaccgtga gtcaggtgac cgccatgcgc caggttcata cccagaacgg tatcacctgg
    32101 ttccagcagc gcggtgtaga ccgcaaagtt agcctgggag ccggagtgcg gctggacgtt
    32161 agcgtagtca gcgccgaaca gttctttcgc acgatcgatc gccagttgtt caacgatatc
    32221 aacatactcg caaccgccgt agtagcgttt gcccggataa ccttcagcat atttgttggt
    32281 cagctgagaa ccctgcgcct gcattacgcg cgggctggtg tagttttcgg aggcgatcag
    32341 ttcgatgtgc tcttcctgac gtactttttc ctgctccata gcctgccaca gttcggcatc
    32401 ataatcggca atgttcattt cacgctttaa catccgcatc tcctgactca gctaacaata
    32461 aaatttttgg cctttatagg cggtcctgtt ggacaacggc gaacagtata accgaatcat
    32521 tgtgcgataa caggtcttga caaaggaatt tacgcaaacg attaccttca ggctacgcaa
    32581 ggctttggag aataaagagc ttgcaaccgg aaacggattt cttttcaggt ttgtgatgca
    32641 aatttttcac ttcatcacat tctttctgaa aaacaccaaa gaaccattta cattgcaggg
    32701 ctatttttta taagatgcat ttgagataca tcaattaaga tgcaaaaaag gaagaccata
    32761 tgcttgacgc tcaaaccatc gctacagtaa aagccaccat ccctttactg gtggaaacgg
    32821 ggccaaagtt aaccgcccat ttctacgacc gtatgtttac tcataaccca gaactcaaag
    32881 aaatttttaa catgagtaac cagcgtaatg gcgatcaacg tgaagccctg tttaacgcta
    32941 ttgccgccta cgccagtaat attgaaaacc tgcctgcgct gctgccagcg gtagaaaaaa
    33001 tcgcgcagaa gcacaccagc ttccagatca aaccggaaca gtacaacatc gtcggtgaac
    33061 acctgttggc aacgctggac gaaatgttca gcccggggca ggaagtgctg gacgcgtggg
    33121 gtaaagccta tggtgtactg gctaatgtat ttatcaatcg cgaggcggaa atctataacg
    33181 aaaacgccag caaagccggt ggttgggaag gtactcgcga tttccgcatt gtggctaaaa
    33241 caccgcgcag cgcgcttatc accagcttcg aactggagcc ggtcgacggt ggcgcagtgg
    33301 cagaataccg tccggggcaa tatctcggcg tctggctgaa gccggaaggt ttcccgcatc
    33361 aggaaattcg tcagtactct ttgactcgca aaccggatgg caaaggctat cgtatcgcgg
    33421 tgaaacgcga agagggtggg caggtatcca actggttgca caatcacgcc aatgttggcg
    33481 atgtcgtgaa actggtcgct ccggcaggtg atttctttat ggctgtcgca gatgacacac
    33541 cagtgacgtt aatctctacc ggtgttggtc aaacgccaat gctggcaatg ctcgacacgc
    33601 tggcaaaagc aggccacaca gcacaagtga actggttcca tgcggcagaa aatggcgaag
    33661 ttcacgcctt tgctgatgaa gttaaggaac tggggcagtc actgccgcgc tttaccgcgc
    33721 acacctggta tcgtcagccg agcgaagccg atcgcgctaa aggtcagttt gatagcgaag
    33781 gtctgatgga tttgagcaaa ctggaaggtg cgttcagcga tccgacaatg cagttctatc
    33841 tctgcggccc ggttggcttc atgcagttta ccgcgaaaca gttagtggat ctgggcgtga
    33901 agcaggaaaa cattcattac gaatgctttg gcccgcataa ggtgctgtaa tttgatgttg
    33961 ccggatggaa acatccggca acccttgacg cggtttaaat tgccgcgtcg tcctcttcac
    34021 cggtacggat gcgaatgacc cgagccacgt caaagacgaa gattttaccg tcaccgattt
    34081 tgccggtttg cgccgtgcga ataatggtat cgacacaggt atcgacaatg tcgtccggta
    34141 cgacaatctc aattttcact ttcggcagaa aatccaccat atactccgcg ccgcggtaca
    34201 gctcggtatg gcctttctgg cgaccaaagc ctttcacttc ggtcaccgtc atgccggtaa
    34261 taccgacttc ggccagtgct tcgcggacat cgtccagctt gaagggtttt ataatcgcat
    34321 caatcttttt catgctattc cttgaaaagg ttgcctgtct tttgatctgc taaacgtaac
    34381 acataacgcc aattcattcc ttgaaatcgt ttgcatccag ctcgtgtcgg gaaagcagtt
    34441 tataaaattc tgtccggttg cgccccgcca ttctcgccgc gtgggtgacg ttgcctttgg
    34501 tgatttgcag cagcttacgc aagtagttga gttcaaaatg attacgtgcc tcaacaaagg
    34561 ttggcagcgc cgtattttca ccctccagcg cctgctccac cagcgcatca ctaatcaccg
    34621 gagatgaggt cagcgccacg cactgttcaa tcacgttgac caactggcgc acattacccg
    34681 gccagctcgc ggtcatcagg cgtttcatcg catcggtaga gaacgcgcgg acaaacggtt
    34741 tatgtcgctc tgccgcctgg cgcaacaggt gatttgccag tagcggaatg tcttctgtgc
    34801 gctccgccag tgccggaatt ttcaggctga caacgttgag gcggtaatag agatcttcgc
    34861 ggaattcccc gcgcgccatc gcttttgaca gatcacggtg agtggcagaa ataatccgca
    34921 cattgatatc aatatcgcgg ttactgccca gcgggcgcac tttacgctcc tgcaacacgc
    34981 gcagcagttt gacctgtaac ggcgcaggca tatcgccaat ttcatcgaga aatagcgttc
    35041 cgccttccgc cgcctggaat aaaccttcgc gattgctgac agcgccagta aacgcgccac
    35101 gcgcatgacc aaacagctcc gactccagca attgctcggg caatgcgcca cagttaatag
    35161 caataaatgg tttgctgttg cgcgggctgg cgttgtggat agcctgggcg aaaatctctt
    35221 tcccggtgcc gctctgaccg ttaatcaaaa cgctgacgtc tgattgcgcc accagccgcg
    35281 cctgttccag caaacgcagc atcagcgggc tgcgggtgac aattgcctcg cgccagcgtt
    35341 catcggtggc tggcgcggat tgctccagcg catcgtcaat tgcctgatat agcgcatctt
    35401 tatcgacagg cttggtgagg aaactaaaaa cgccctgctg tgttgcagca acggcatcgg
    35461 ggatagaacc atgcgcagta agaataatta ctggcattcc cggctgcact ttctggattt
    35521 cagcaaatag ctgcataccg tccatttcat ccatccgcag gtcgctgata actaaatcta
    35581 ctttttcgcg attcagtacc cgtaatcctt cagcgccact ttccgccgtg accacactgt
    35641 agccttcgct ggtcaggcgc aggccaagca gtttcagcaa tcccggatcg tcatcgacca
    35701 ataataaatg cgcaggttta tggctcatca ggagtgacct catgggtgga tggcgcgggc
    35761 ttttcactct cgtgcggcgt atccggcgag aaatttccag ccggtttgcg ggtcgagagc
    35821 tggcgttcaa tatcggtcag gttttccagc ttgcgggtgg tgagttccag ttgctgctgt
    35881 agaacgtgat gttgctggcg caatgtatcc agctcgctgt cgctggactg ctggagttta
    35941 ctgtaacgtt ggcgctcttc cgccagttgc agttgcagcg cctgaccgtc gcgccagagt
    36001 tgatacagtg ggcgaacctg tgccgggatc tcggtactta acgcttcaat acgcgcgacc
    36061 agctggcggc gctcatacgg cgtaattttg gcgtcggcga gcaaaatccc ttgtttaaag
    36121 gtattttgcc agctgccgtc gtcatattgg cgagcttgct gacgcgactg cgcaggcatt
    36181 aaacgatcag cacaatccat cgcccgcagc cagtaaagcg gattggtttc ggttgattta
    36241 ccttgcagcg cccagatgtc gctacattca gtagaaagat agtcagccag ttgataaacc
    36301 ggaatttttt cttctgctgg cgtatcaatg gctggcttat tgtgattctg cacgcaaccc
    36361 agcaatgcca gacatggcag ccctgccagc cacagccgtc ggggcaataa tcgttgaaaa
    36421 atgtgtcgca tattcaccag acttaaagcc tatcccagtg ggcgtaattg ttgcagacag
    36481 tctggacatg gacagcgcgg agaaaccggg gcgtacatat cgtacgtgaa gatttcgagc
    36541 actacccggg gccgaactga caaataaaat agcctgatag gataggctgt tagcattatt
    36601 tcgtgttttt cgacgacggt aattcaatgc ggaaacaaac gtcttgcccg ctctcgtcga
    36661 ccagatacag ttccccttgc atacggcgaa tacaatccct ggcaatgctt aatcccagac
    36721 cgctgccctt caccgcccct tttcgctggt ggcttccctg aaaaaagggt tcgaagatca
    36781 tggcgcgttc ctcttgcgga atgggcgtgc ctgtattgat gacatcaata taaacccgcg
    36841 caccatgtaa actgctgcga aggcaaatgt taccggattc agccccgtag tgcaccgcat
    36901 tggagtaaag attatccagt acgctcatca gcagcattgg ctccgccagg caagctgttg
    36961 ctttgagatc gacgtcggta tgcatcattt tagcccgtgc gggcaggcta tgagcagaaa
    37021 ccactgtctc caccagcggt gctaattcaa cattctccag ttccaccgca ctgtccgcct
    37081 gtttacggtt gtaatcaagc agttgttcga tcagtttttg caaattgcag ctgctgctat
    37141 caagaatgct caccacctct ttttgctctg gcgtaagcgg cccgacaacc tgatcagcca
    37201 gtaattcagt gccctcgcgc atactcgcca gtggcgtttt taattcatga gataaatgtc
    37261 ttaaaaattg atggcgttgg gattccagcc atgacaggcg ctcacttaac caaagaatac
    37321 gttgcccaac cgagcgtaac tcgctcggtc cacagaacga aacgctattg cccagagaac
    37381 gcccttcccc cagacggttg atcatgcgct cgatattttt caccggcccg ataatcatcc
    37441 gcgtgaaaag tagcaccatc accagactca ccagaaatag caccagcgat tgccaaccaa
    37501 aatattgccc acgttcggcg atttcacgct gaagttgctg cccacgagag aacaccactg
    37561 tgcgcgtggc ctgcaccatt tcggtattgg cactggcaaa ggcttcaaga cgcgcggcgg
    37621 cggcagcatc gggaccactg ttgttacact gaagttgggc cagattgttc aaatcctgac
    37681 gcaatgcctg gtaaagttta tcgtctggca gtacgcctgc gtgggcatcg agcatttcgc
    37741 tgtaacgctt gcgctggctt tgataaacct tcgcaagcgt agggtcgtcc agcacgcaat
    37801 actgacggta gctacgctcc atctccagcg ccgcgttggt catcgcttca ctacgccggg
    37861 catcaataag cgtagtgcgg ttaaccagcg ccgcctgatc gctaagcgca ttcaggcttt
    37921 gccaggcttg ccatgccagc accaacaggg gtagtagaat tagcaaaaat gccagcatta
    37981 ccagttgtcg taatgagcgg ggaaaaacgg gccagcgttt caaggtgtta ctctcgtcag
    38041 acgcgaatag cctgatgcta accgagggga agttcagata caacaaagcc gggaattacc
    38101 cggctttgtt atggaataag gcggtgccta actcgacgtt tcgcccgatg gttgatatag
    38161 ctacgctgat atcagaagct ggacggcagg caccttgttg tgcgtcattc gaattttatg
    38221 tagcacgtcc cgaaggggct gacataagtc ggtgaatgag ccactgctta ctattatgca
    38281 gtaactgtgc caataaagaa aatagtttgg taacgtactg attatacgtt gctttgaggg
    38341 atttatggct tcttcgctat ttgaatgtaa atcagccact gtgtcgctaa aaagagacaa
    38401 cttaagataa acttattaga ttgcaataaa atcaatcatt taagtgtcgc cagaaagcga
    38461 cacggtaaac cacccattgt cgtgatttac agacacaaaa aagctcccgg agttgggagc
    38521 ttatgatagt ggttggtgct taatgccgca tccgggctgc aaaaccaatg aactgatgac
    38581 ttaccccaac tgcttacgcg cattgcggaa aatgcgcatc catgggccat cctcgcccca
    38641 gttttccgga tgccaggagt tgctgacagt acggaaaaca cgttccgggt gcggcatcat
    38701 aatggtgact cgaccacttt cagtcgtaac cgccgtaata ccgttcggtg aaccgttcgg
    38761 gttagccggg taggtttcag tgactttgcc gaagttatcg acatagcgca gtgccaccag
    38821 ccctttgctt tccagtgccg ccagatgcgc cgcatcacgc acttccacgc gcccttcacc
    38881 atgagagaca gcaatcggca tttgcgagcc caccatcccc tgcaacagca gagacgggct
    38941 ttgggttact tcaaccaggc tgaaacgcgc ttcaaagcga tcggaggtgt tacgcacaaa
    39001 acgtggccac aactcactcc ccgggatcag ttcacgcagg ttagacatca tctggcaacc
    39061 gttacatacc cccagggcca gcgtttgcgg acgatggaag aaggttgcaa actcatcgcg
    39121 tacacggtca ttgaacagga tagacttcgc ccaaccttca ccggcaccca gcacatcacc
    39181 gtaggagaaa cctccgcacg cgaccagggc gtgaaaatcc tccagtcccg tacgtccagt
    39241 cagcaggtcg ctcatatgca cgtcgatagc atcaaagccc ccacgttgga aagctgccgc
    39301 catttcaaca tgcgagttca cgccctgctc acgcagtacg gcaactttcg gacgtgcgcc
    39361 agtggcaata tacggtgctg ccacatcttc gttgatatcg aacgacagtt ttacattcag
    39421 gcccggatcg gcgtcgttag atttcgcctg atgctcctga tcggcacact ccgggttgtc
    39481 acgcaggcgc tgcatctgcc aggtagtttc tgcccaccag acacgcaacg tagtgcggct
    39541 ttcgctgaat acagtctgcc cgttggcggt aatcacaaaa cggtcaccgg aaaccgcctg
    39601 cccaacataa tggacgcaat ccgcaagccc atgctgtgcc agtacggact cgaccgcttc
    39661 acggtcagcg gcacgaacct gaatcaccgc acccagttct tcgttaaaca acgccgccag
    39721 gcgatcgtca cccagagagg cgatatccgc gtcaatgcca caatgacctg caaaggccat
    39781 ttccgccagc gttaccagca ggcctccatc agagcggtcg tgatacgcca gcagcttacg
    39841 ctgtgcaacc agcgcctgaa tcgcgtcata gaagcctttc agttggacaa catcgcgtac
    39901 atctgccggt ttgtcgccaa gctgacgata aacctgcgcc agcgccgttg cgcccagcgc
    39961 gttattgcct ttgcccaaat caatcagcag cagtgcgtta tcttcggtac aaagctgcgg
    40021 cgtgatggtg tgacgtacat cttccacgcg ggcaaatgca gaaatcacca gcgacagcgg
    40081 cgacgtcatt tcgcgctctt cgttaccttc ctgccagcgg gttttcatcg acatagagtc
    40141 tttacccacc gggatcgtca ggcccagcgc cggacaaagt tcttcaccca cggctttaac
    40201 ggcttcatac aggcccgcat cttcaccagg gtggcctgcc gccgccatcc agttggcgga
    40261 aagtttaatg cgtttgatat cgccaatctg tgttgcggca atattggtta acgcttcacc
    40321 gaccgccaga cgggcagagg cggcgaaatc cagcagcgca accggcgcac gctcgccaat
    40381 cgccatcgct tcaccgtagt agctgtcgag gctggcggta gtgaccgcgc agttagcgac
    40441 cggcacctgc cacggcccca ccatctgatc gcgcgccacc atgccggtta cgctgcggtc
    40501 accgatggtc accaggaaag ttttctccgc cacggtcggc agatgcagta cgcgtttcac
    40561 cgcgtctgca atggtgatcc cttcacggac cagcgcatcg cccttcgctt tcagcgtttg
    40621 tacatcgcgg gtcatcttcg gcgttttacc aagcaggacg tccagcggca gatcgatcgg
    40681 ctgattatca aaatgacgat cgtgcagaga aagatgcagt tcttcggtcg cttcaccaat
    40741 caccgcgtag ggtgcgcgct cacgcttaca cagttcgtca aacagcggta attgatcggc
    40801 agcaaccgcc agcacgtagc gttcctggga ttcgttacac cagatttcca gcgggctcat
    40861 gcccggttcg tcgcttagaa tatcgcgcag ttcaaattta ccgccgcgtc cgccgtcgct
    40921 caccagctcc ggcatcgcgt tagaaagacc gcccgcaccg acgtcgtgga taaacagaat
    40981 tgggttagca tcaccaagct gccagcaacg gtcgatcact tcctggcaac gacgctccat
    41041 ctccgggttg tcgcgctgta cggaagcaaa gtcgaggtcg gcatcagact gaccagacgc
    41101 catggaagac gccgcgccgc cgccaagacc gatgttcatt gccgggccgc cgagaacgac
    41161 cagcttcgca ccgacgttga tctcgccttt ttgtacgtga tcggcgcgaa tgttgccgat
    41221 cccgcccgcc agcatgatcg gtttgtgata accgcgcagc tcttcgccat tgtggctgtt
    41281 cactttttct tcataagtac ggaagtagcc gttcagtgcc ggacgaccaa attcgttgtt
    41341 aaacgccgca ccgcccagcg ggccttcggt catgatgtcc agcgcggtaa caatgcgctc
    41401 aggcttaccg aaatcttctt cccacggctg ttcgaagcca ggaattcgca ggttggatac
    41461 ggagaaacca accaaaccag ctttcggctt cgcgccgcgc ccggtggcac cttcatcgcg
    41521 gatttcaccg ccggaaccgg tcgccgcgcc cggccacgga gaaatcgccg tcgggtggtt
    41581 gtgagtttcg actttcatca ggatgtgcgc cggttcctga tggaaattgt agcggcccgt
    41641 ttcgtggtca gcaaagtagc ggcccacttc agaaccttcc attacggcgg cgttatcttt
    41701 ataagcagag agaacgtgat ctggcgtggt ttcgaaagta tttttgatca tcctgaacag
    41761 cgatttcggc tgttgttcac cgtcgataat ccagtcggcg ttaaaaattt tgtggcggca
    41821 gtgctcagag ttcgcctgag cgaacatata cagctcgatg tcgttcgggt tacgaccaag
    41881 ctttgtgaaa gcatcctgca gatagtcaat ttcatcttcc gccagagcca agccaagacg
    41941 caggttagcg tcgatcagcg cctgacggcc ctgccccagc aaatcaacgc ttgtgaccgg
    42001 agtcggttga tggtgggcaa acaactgctc tgcatcatct aaatcaaaaa agaccgtttc
    42061 catcatgcgg tcgtgcagtt cagcggtaac ctgctgccat tgttcattgg tcagcgtacc
    42121 ggcttctata tagtaagcaa cgccgcgctc aaggcggttt acctgttgta gcccgcagtt
    42181 atgggcaata tcggtcgctt tcgaagacca gggagagatg gtgccaggac gcggggtcac
    42241 cagcaggagt ttgccttgcg gggcgtggct ggcgagtgcc ggaccatatt tcagcaggcg
    42301 ttcaagttgt gcgtgctcat catcgtttaa cggcgcattg aggtcagcaa aatggacata
    42361 ctcggcgtaa atattgtgaa ccgggagcct ggcagcctga aaacgtgcca gcagtttgtt
    42421 gattcggaat gccgacagtg caggcgaacc acgcagaatt tccatcataa gtctctcgtc
    42481 ttctaagctt tcggtgtacc caaggggggg gaaacgggcg tcattataaa gaatctgatg
    42541 cgctgacgaa accgtttgcg tggaaataaa atcaccatcg tgaattagca acgcgtgccg
    42601 ccaatggctg taataagttg ccatctggcg caggtttacg caaaatgccg ctcatttatg
    42661 agtaaacctt tcactattat tacgtttttt caagctggga cgcgcacgac acagagaatt
    42721 aactaattga aaaaattaaa gattaattat ctgttcatcg gcattctggc actgctgctc
    42781 gcggtcgctc tctggccatc cattccctgg tttggtaaag ccgacaaccg tatcgccgcc
    42841 attcaagcgc ggggagagtt gcgtgtgagc accattcata ctcccctgac ttataacgaa
    42901 atcaacggga aaccttttgg cctggattac gaactggcga aacagtttgc cgattacctc
    42961 ggcgtaaaac tgaaagtgac cgtgcggcag aatatcagcc agctgtttga cgaccttgat
    43021 aatggtaacg ccgacctgct ggcggcagga ctggtctata acagtgagcg ggtaaaaaat
    43081 tatcagcctg gccctaccta ttattccgtg tcacaacaac tggtttataa agtgggtcag
    43141 tatcgcccac gtacgctggg caacctgacg gcggagcaac tcaccgttgc accgggtcat
    43201 gtggtagtta acgatctcca gaccctgaaa gaaacaaaat tcccggaatt aagctggaag
    43261 gtagacgaca aaaaaggctc tgcggaatta atggaagatg tcatcgaagg aaaactcgat
    43321 tacaccattg ctgattctgt cgccatcagc ctgtttcagc gcgttcaccc ggagctcgcc
    43381 gtagcgctcg atatcaccga tgaacaaccg gtgacttggt ttagcccgtt agatggcgat
    43441 aatacccttt ccgccgccct gctcgacttc ttcaacgaaa tgaatgaaga cggtacgctg
    43501 gcacgcattg aagagaaata cctggggcat ggcgatgatt ttgattacgt cgatacgcgc
    43561 acatttttac gcgccgtcga tgcggtactg ccgcagttaa agcccctgtt tgagaaatac
    43621 gccgaagaaa ttgactggcg tttgctggcc gctattgctt atcaggaatc gcactgggat
    43681 gcacaggcca cttcaccgac gggtgtgcgc ggcatgatga tgttaaccaa aaataccgcg
    43741 caaagcctcg gcattacgga tcgtaccgat gccgaacaga gcatcagcgg tggcgtgcgt
    43801 tatttgcagg atatgatgag taaagtgccg gaaagtgtgc cggagaacga acggatctgg
    43861 tttgccctcg ctgcgtacaa tatgggctat gcgcatatgc tggatgcccg cgccctgacg
    43921 gcaaaaacca aagggaatcc tgacagttgg gctgacgtaa aacagcgtct gcctttactt
    43981 agccagaaac cctattacag caagctgact tacggctacg ctcgtgggca tgaagcctac
    44041 gcttatgtcg aaaatattcg taagtatcag attagcctgg tgggttatct gcaagagaaa
    44101 gagaagcagg ctacagaagc ggcgatgcaa ctggcgcagg attatccggc ggtatcgcct
    44161 acggagttgg gcaaagagaa atttcctttt ctctcgtttc tttcccagtc gtcatcaaac
    44221 tatttgaccc attctccctc tctgctgttt tccaggaaag ggagtgaaga gaaacaaaat
    44281 taatccgtcg aggattgcgc ttttttctgc gctttaattt cctggcggcg catgcgaaag
    44341 aagtcactga gcaacgccgc gcactcatcc gccagtattc cttccgtaat ttccactcgg
    44401 tgattcatac ccggatgatg cagcacatcc attaaagatc ccgcagcgcc agttttcgcg
    44461 tcacgcgcac caaagaccac gcgaccaatg cgactgtgga tcatcgctcc ggcacacatt
    44521 acacatggtt caagcgtgac atacaacgtg gcgtcgatca gacgataatt ttgcatcacc
    44581 agaccaccct gccgcagggc catgatttct gcatgtgcgg tgggatcatg gcgaccaatc
    44641 gggcggttcc agccttcgcc gattacccga ttgttatgca ctaataccgc gccgaccggc
    44701 acttcccgct catcccaggc acgtttcgcc agcgtcagcg cgtgacgcat ccaatattcg
    44761 tggctaaatt cgacttcaga caaaaagaaa actccggtta taaaagcgcg gcgcattata
    44821 cacggacgct atgctttact ctattccagt tgctggagtt caccgcgcgg ggttactcgc
    44881 caacgatgct gacagaaata aagcagcgga ttgtcctgat tactgtcgct atagccactg
    44941 tacagccgca gcggcgtgcc gattttgcgc tccagttgtg cgaccttttc atgtcccaga
    45001 caacgcatcg tcaataccca accaccatag ccacgctgaa tttggctggc gataagatta
    45061 acccgcggca gccagggcgt atcgaaataa accgcttcaa ccagcggctg cggagagccg
    45121 gtaatcaacc agatatcagc atcggaactt aacaggtagg tggttaatcg ctcctgaacc
    45181 agcggaaagg cggtaacatt gtcgcgaaac cagcgcacga aatcggcctg caacgtctgt
    45241 aaacgtgctt cgctgtgacc aaaagtgcac ccccacagaa gcagactcat cggccagcgt
    45301 gccgcacgac cttttatcaa taacgcaatg gctataatcg gtaacaacgg caggacaagt
    45361 aacgcattca gcggttggcg acgcagtaaa tagcgcagaa aactgccgaa catatcctgc
    45421 tgatgcaatg ttccatctaa gtcaaaaaac accacacgac gctcgtgagt tgccaaacca
    45481 tactcctctg gactgaaaca tcctgattaa ttttcgctac tacatagcct aacagataga
    45541 tcatcacttt tccggcaaca atcgaccgca tcatggctaa ctggaatttt taattcatgg
    45601 caattagcgg caatggaata taaaattcac tcgcgtgtgt ctcatattgc ctgttgtcgc
    45661 cactatttca tcgcgaggaa agatatgaac ggcttacttc gtatccgtca gcgttaccaa
    45721 gggcttgccc agagtgataa aaaactggcg gattatctgc tgctacaacc tgatacggcg
    45781 cgccatttaa gctctcagca actggccaac gaagccggag tcagtcagtc cagcgtcgtg
    45841 aagttcgcgc aaaaactcgg ctataaaggt tttccggcgc ttaagttggc gttgagtgaa
    45901 gcgctggcaa gccagccgga atcgccctcc gtgcccattc ataaccaaat ccgcggtgat
    45961 gatccgttac ggctggtcgg cgaaaaactg attaaagaaa ataccgccgc tatgtacgca
    46021 acgctaaacg ttaatagtga agagaaactg catgaatgcg taacaatgtt gcgctctgcg
    46081 cggcggataa ttctgaccgg tattggcgct tcgggtctgg tggcgcaaaa ctttgcctgg
    46141 aagctgatga agattggctt caatgctgcc gcactgcgcg atatgcatgc gctactcgca
    46201 acagtacagg cgtcgtcccc tgacgatctg ttattagcca tttcctacac cggtgtacga
    46261 cgcgagttaa acctggcggc agatgagatg ctgcgagtgg gcggaaaagt gctggcgatt
    46321 accggcttta ctccgaatgc cctgcaacag cgtgcttctc attgcctgta taccattgcc
    46381 gaagaacaag cgacaaacag tgcttcaatc tctgcttgtc acgctcaggg aatgttaacg
    46441 gatttgctgt tcattgcgct gattcagcag gatctggaac tggcaccaga acgtattcgt
    46501 catagtgaag cgctggtgaa aaaactggtc tgagtaaaga atgcgcgtat aatgcccgcc
    46561 cggtttgtgt tgttttgaga gtttccttat ggcgctgtta atcactaaaa aatgcatcaa
    46621 ttgtgatatg tgtgaaatca aataaaaaca catttatttt catagtgata cagcaatgtg
    46681 ttattgaatg tgttgttgga tttatggtgc aaaaaaattt tgcataagtt cagcaacaca
    46741 aagaacacca ctttctttaa cgtcctccgc gccctaaagg acggggcttt acggcgcaaa
    46801 aggcctgtga catacaacat taacttttca gtcaaccgta gcacgttctt gtatacgccg
    46861 tgtctgcggt tttatataac cctggctgga aacctcttat acaaagtcga ttcgccaaca
    46921 tcatagatga tcgccacctt ctggcgagaa actcctgatg caattagtcg cccggcctgc
    46981 gcccattatt ctggtgaaag tttgggacgg cgaccgccaa ttcgtccctg tgcgcgagca
    47041 gcttccagtc ctgcttttgt tcgttcaaca atcagttcac gttccatttc agccagggca
    47101 cccatcacat ggaagaaaaa gcgtcccatt ggtgtgctgg tatcaattga atcagtcaga
    47161 ctacgaaagt tgatgcctcg ttcgcgcaac gcctccacca gcacgacaag atgccgcata
    47221 ctgcgcccca gccgatccag cttccagaca accagtgtgt cgcctgccga taatgtcttg
    47281 agcagttttt tcagccccgg tctttcggac tttgtaccac ttatcttgtc ttcaaaaatc
    47341 agctcgcatc ctgcacagtt caa
//
LOCUS       DAZPAB010000010        44653 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZPAB010000010 DAZPAB010000000
VERSION     DAZPAB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.010"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(315..857)
                     /gene="ycfP"
                     /locus_tag="ACHUPG_000502"
     CDS             complement(315..857)
                     /gene="ycfP"
                     /locus_tag="ACHUPG_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR0860253.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(880..1905)
                     /gene="nagZ"
                     /locus_tag="ACHUPG_000503"
     CDS             complement(880..1905)
                     /gene="nagZ"
                     /locus_tag="ACHUPG_000503"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR0860254.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(1916..2740)
                     /gene="thiK"
                     /locus_tag="ACHUPG_000504"
     CDS             complement(1916..2740)
                     /gene="thiK"
                     /locus_tag="ACHUPG_000504"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR0860255.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2721..3362)
                     /gene="lpoB"
                     /locus_tag="ACHUPG_000505"
     CDS             complement(2721..3362)
                     /gene="lpoB"
                     /locus_tag="ACHUPG_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR0860256.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3377..3754)
                     /locus_tag="ACHUPG_000506"
     CDS             complement(3377..3754)
                     /locus_tag="ACHUPG_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR0860257.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3757..4116)
                     /gene="hinT"
                     /locus_tag="ACHUPG_000507"
     CDS             complement(3757..4116)
                     /gene="hinT"
                     /locus_tag="ACHUPG_000507"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR0860258.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4449..6634
                     /gene="fhuE"
                     /locus_tag="ACHUPG_000508"
                     /pseudo
     CDS             4449..6634
                     /gene="fhuE"
                     /locus_tag="ACHUPG_000508"
                     /inference="COORDINATES: protein motif:HMM:NF007447.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(6694..8127)
                     /gene="ptsG"
                     /locus_tag="ACHUPG_000509"
     CDS             complement(6694..8127)
                     /gene="ptsG"
                     /locus_tag="ACHUPG_000509"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR0860259.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8422..9219)
                     /locus_tag="ACHUPG_000510"
     CDS             complement(8422..9219)
                     /locus_tag="ACHUPG_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0860260.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9230..10234)
                     /gene="holB"
                     /locus_tag="ACHUPG_000511"
     CDS             complement(9230..10234)
                     /gene="holB"
                     /locus_tag="ACHUPG_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR0860261.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10231..10872)
                     /gene="tmk"
                     /locus_tag="ACHUPG_000512"
     CDS             complement(10231..10872)
                     /gene="tmk"
                     /locus_tag="ACHUPG_000512"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR0860262.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(10862..11884)
                     /gene="yceG"
                     /locus_tag="ACHUPG_000513"
     CDS             complement(10862..11884)
                     /gene="yceG"
                     /locus_tag="ACHUPG_000513"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR0860263.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11887..12696)
                     /gene="pabC"
                     /locus_tag="ACHUPG_000514"
     CDS             complement(11887..12696)
                     /gene="pabC"
                     /locus_tag="ACHUPG_000514"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR0860264.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(12816..14057)
                     /gene="fabF"
                     /locus_tag="ACHUPG_000515"
     CDS             complement(12816..14057)
                     /gene="fabF"
                     /locus_tag="ACHUPG_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR0860265.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14144..14380)
                     /gene="acpP"
                     /locus_tag="ACHUPG_000516"
     CDS             complement(14144..14380)
                     /gene="acpP"
                     /locus_tag="ACHUPG_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR0860266.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14591..15325)
                     /gene="fabG"
                     /locus_tag="ACHUPG_000517"
     CDS             complement(14591..15325)
                     /gene="fabG"
                     /locus_tag="ACHUPG_000517"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR0860267.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15338..16267)
                     /gene="fabD"
                     /locus_tag="ACHUPG_000518"
     CDS             complement(15338..16267)
                     /gene="fabD"
                     /locus_tag="ACHUPG_000518"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR0860268.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16283..17236)
                     /gene="fabH"
                     /locus_tag="ACHUPG_000519"
     CDS             complement(16283..17236)
                     /gene="fabH"
                     /locus_tag="ACHUPG_000519"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR0860269.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17304..18374)
                     /gene="plsX"
                     /locus_tag="ACHUPG_000520"
     CDS             complement(17304..18374)
                     /gene="plsX"
                     /locus_tag="ACHUPG_000520"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR0860270.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18455..18628)
                     /gene="rpmF"
                     /locus_tag="ACHUPG_000521"
     CDS             complement(18455..18628)
                     /gene="rpmF"
                     /locus_tag="ACHUPG_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR0860271.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18680..19201)
                     /gene="yceD"
                     /locus_tag="ACHUPG_000522"
     CDS             complement(18680..19201)
                     /gene="yceD"
                     /locus_tag="ACHUPG_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR0860272.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            19400..19984
                     /gene="yceF"
                     /locus_tag="ACHUPG_000523"
     CDS             19400..19984
                     /gene="yceF"
                     /locus_tag="ACHUPG_000523"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR0860273.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20096..21055)
                     /gene="rluC"
                     /locus_tag="ACHUPG_000524"
     CDS             complement(20096..21055)
                     /gene="rluC"
                     /locus_tag="ACHUPG_000524"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR0860274.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            21628..24819
                     /gene="rne"
                     /locus_tag="ACHUPG_000525"
     CDS             21628..24819
                     /gene="rne"
                     /locus_tag="ACHUPG_000525"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR0860275.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(25016..25958)
                     /gene="flgL"
                     /locus_tag="ACHUPG_000526"
                     /pseudo
     CDS             complement(25016..25958)
                     /gene="flgL"
                     /locus_tag="ACHUPG_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(25970..27612)
                     /gene="flgK"
                     /locus_tag="ACHUPG_000527"
                     /pseudo
     CDS             complement(25970..27612)
                     /gene="flgK"
                     /locus_tag="ACHUPG_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(27678..28619)
                     /gene="flgJ"
                     /locus_tag="ACHUPG_000528"
     CDS             complement(27678..28619)
                     /gene="flgJ"
                     /locus_tag="ACHUPG_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR0860276.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28619..29716)
                     /gene="flgI"
                     /locus_tag="ACHUPG_000529"
     CDS             complement(28619..29716)
                     /gene="flgI"
                     /locus_tag="ACHUPG_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR0860277.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29728..30426)
                     /gene="flgH"
                     /locus_tag="ACHUPG_000530"
     CDS             complement(29728..30426)
                     /gene="flgH"
                     /locus_tag="ACHUPG_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR0860278.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30479..31261)
                     /gene="flgG"
                     /locus_tag="ACHUPG_000531"
     CDS             complement(30479..31261)
                     /gene="flgG"
                     /locus_tag="ACHUPG_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFR0860279.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(31399..31986)
                     /gene="flgF"
                     /locus_tag="ACHUPG_000532"
                     /pseudo
     CDS             complement(31399..31986)
                     /gene="flgF"
                     /locus_tag="ACHUPG_000532"
                     /inference="COORDINATES: protein motif:HMM:NF009280.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(32088..32150)
                     /locus_tag="ACHUPG_000533"
                     /pseudo
     CDS             complement(32088..32150)
                     /locus_tag="ACHUPG_000533"
                     /inference="COORDINATES: protein motif:HMM:NF012674.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(32170..33309)
                     /locus_tag="ACHUPG_000534"
     CDS             complement(32170..33309)
                     /locus_tag="ACHUPG_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR0860280.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGANNIVATTQNGYKPGDLVSYQINDDGTV
                     VGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTN
                     GALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR"
     gene            complement(33334..34029)
                     /gene="flgD"
                     /locus_tag="ACHUPG_000535"
     CDS             complement(33334..34029)
                     /gene="flgD"
                     /locus_tag="ACHUPG_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR0860281.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34041..34444)
                     /gene="flgC"
                     /locus_tag="ACHUPG_000536"
                     /pseudo
     CDS             complement(34041..34444)
                     /gene="flgC"
                     /locus_tag="ACHUPG_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(34448..34864)
                     /gene="flgB"
                     /locus_tag="ACHUPG_000537"
     CDS             complement(34448..34864)
                     /gene="flgB"
                     /locus_tag="ACHUPG_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR0860282.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35019..35678
                     /gene="flgA"
                     /locus_tag="ACHUPG_000538"
     CDS             35019..35678
                     /gene="flgA"
                     /locus_tag="ACHUPG_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR0860283.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            35754..36047
                     /gene="flgM"
                     /locus_tag="ACHUPG_000539"
     CDS             35754..36047
                     /gene="flgM"
                     /locus_tag="ACHUPG_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR0860284.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            36052..36468
                     /gene="flgN"
                     /locus_tag="ACHUPG_000540"
     CDS             36052..36468
                     /gene="flgN"
                     /locus_tag="ACHUPG_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR0860285.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(36508..38043)
                     /gene="murJ"
                     /locus_tag="ACHUPG_000541"
     CDS             complement(36508..38043)
                     /gene="murJ"
                     /locus_tag="ACHUPG_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR0860286.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38210..39076)
                     /locus_tag="ACHUPG_000542"
                     /pseudo
     CDS             complement(38210..39076)
                     /locus_tag="ACHUPG_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(39078..39725)
                     /locus_tag="ACHUPG_000543"
     CDS             complement(39078..39725)
                     /locus_tag="ACHUPG_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HFR0860287.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39736..40320)
                     /gene="rimJ"
                     /locus_tag="ACHUPG_000544"
     CDS             complement(39736..40320)
                     /gene="rimJ"
                     /locus_tag="ACHUPG_000544"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR0860288.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40556..41764
                     /gene="mdtH"
                     /locus_tag="ACHUPG_000545"
     CDS             40556..41764
                     /gene="mdtH"
                     /locus_tag="ACHUPG_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR0860289.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            41828..42475
                     /gene="grxB"
                     /locus_tag="ACHUPG_000546"
     CDS             41828..42475
                     /gene="grxB"
                     /locus_tag="ACHUPG_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFR0860290.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            42609..43169
                     /locus_tag="ACHUPG_000547"
     CDS             42609..43169
                     /locus_tag="ACHUPG_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0860291.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43275..44321
                     /gene="pyrC"
                     /locus_tag="ACHUPG_000548"
     CDS             43275..44321
                     /gene="pyrC"
                     /locus_tag="ACHUPG_000548"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR0860292.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            44395..44640
                     /gene="dinI"
                     /locus_tag="ACHUPG_000549"
     CDS             44395..44640
                     /gene="dinI"
                     /locus_tag="ACHUPG_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR0860293.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        10809 a        12067 c        11102 g        10675 t
ORIGIN      
        1 cgctattgag aataacatga atggtgcatt ggtcatacca aaattgatgt taatcaagtt
       61 ttgttgctta aaagataggc aactttaccg ggggagatca aaaaatagag tttattgttt
      121 attatttgag caaattcgga agggtaatat agggtgaagc gtatatcgag ggcgtttggt
      181 cgtcctgata agatgcgcag catcgcatca ggcattgatg ctcgactgtc ggatgcggcg
      241 tggacgcctt atccgactta cagaattcgg agcgcatcag gtaccggcaa ggttacctga
      301 tgcgacgagg gcatttaccc gagggttttg aacgctttaa tgcgctgtaa atgcggggag
      361 atattcttga atttgtgcgt ctgctcttcg tcccagacaa tctcgtaata atgatgcaac
      421 tcttcagatg tccgctggct gttaagtgct tcatcattac gcgacaaaat aaccagacaa
      481 cgatcgcgat tcttctcgcg gaagttggtc acacacttag tggcaatatc ggcatactct
      541 tccgggcgat caatcttgcc ttccatgttc tcataaggga acaaattagg gttgaagatc
      601 acctggcgga tatcgcaaag aaaaccaatc cgttccgccc agtatccgcc caggccaacg
      661 ccgcaaatta gcggacgctc gtcaacgttc agttgcaaca ttttgtccac ttctttaagc
      721 agatgctgca tatcatgttt cggatgccgc gtactgtagc ttatcaagcg tacatccggg
      781 tcaataaact gcaattgtaa gactttctcg tggttacccg gactgttaga gtcaaaaccg
      841 tgtaaataga taatcatcgc ttcctcacat aagccagggt tagtgaccta ctttctcttc
      901 ctgccagcgt tcatgtaact gattcagacg ggtgctgatc gctttccagc gagccgagtc
      961 catcagttcc tgtcgcgaaa atgaaccttt atgatacaaa cgtgtaacac gttctgcctt
     1021 gatcggtgac agattatcta acacgctgac ggccccttta cgattattgc agaccaggat
     1081 catatcgcaa cccgcatcca gtgatgcctg cccgcgttcg gcataactgc ccataatcgc
     1141 ggcaccttcc atcgataaat cgtcagagaa aatcacgccg tcaaacccca gttcctgacg
     1201 caaaacggtt ttcagccagt agggagaacc gctcgccgga cgcggatcaa catcactgta
     1261 gatcacatgc gcaggcataa tggcgtcgag tttattttcg cggattaagg aactgaagac
     1321 cgacatatct ttagcgcgaa tctccgcttg tggacgtgga tcgcacggtg tttctttgtg
     1381 tgagtctgcc gttactgcac cgtgtcctgg gaagtgtttc ccggtcgttt tcattccggc
     1441 ttcatgcata ccatcaataa accggctggc aattgccagg gctttttgtg gatcggcatg
     1501 ataagaacgc tcgccaatcg ccgcgctgat atgcccgaca tccagcactg gcgcaaagct
     1561 gatatcaata tccatagcga tcatttcgct ggccatcagc caacccgcct cttgcgccag
     1621 tttgccgccc tcttccattc ccaacagcgc agcaaaggat tgtgctgccg gtaagcgggt
     1681 aaaaccttcg cgaaaacgct gcacgcgtcc accttcctga tctaccgcca ccaccagatg
     1741 attgcgcgat gctgcgcgga tctggcgcac cagttcacgt aactgggcag gatcatgata
     1801 gttacgcgta aagagaatca gccctcccac cagcggatgc gccagtattt cacgctcttc
     1861 cgcgtccagt tcgtaacctt cgacatccaa cattactgga cccacactgc tctccttatt
     1921 gttttattaa tagctgccgc caggtgtcat cggccagcct gataaattgt tgatcgccgg
     1981 tttgtcgcca gcggtactca aaccaccctc ctttgagcat cagcagccag ggaaaccatc
     2041 gcctgacctg acgccataat tgcgccggat aaatcttcgc gcgagtggca tagtcattga
     2101 ccaattgccg gtgctgttca gtattttcca cccacaccgc cgccagttcc agcgcgatat
     2161 caccatctcc agcatactcc cagtcgatga gttttaaccc tgatgcgcta tgcactaaat
     2221 ttccggcgtg gacatccata tgcaatggac ttaagcgtaa aggtcgtggt tcccgcgctt
     2281 tgcgcagacg ttttaacatt cgcagccaac ccacagtccg ccgcgccgga tcgctttgct
     2341 gccagtacag ttccagtaag ggcaacagcg ttattcgcca gccaaaacgt ggttgttgat
     2401 gtagataata cagcaagcct gccagttcgt tggtatccgg caaatacgtt tttaccgcac
     2461 cgggcagata gtcgactacc atccagtcac ggagatataa atgcggcttc ggtgcaatgc
     2521 ttgcgggtag ttgtgataaa gcccgatact ggcgcaagaa cgcggactgc ggcgcatcag
     2581 gatcgtgcgg ctgacgcaca acaaaacgct ggccctgatg ttcaatgaga aaactaccgc
     2641 cactaagccc actattaaac gtcgtgacgg gatgaaactg cgggaaaaag cgcgacagca
     2701 attcgtcgcg cgtgatggga ttattgctgc gaaacggcac ctttacctga ccagataatt
     2761 tcgcccgtct gcaccagcat cagctgcatt tgtagggtcg gagcgttaac gttgccagag
     2821 gcgcaggagt acagcacgta atgagcgccg acattgcggg caatgcctat ggctttacta
     2881 cgggtgccta aactgtcctg cggcgacaaa cctaactgtt gcttagccat cgacagttgc
     2941 tgggcggaaa ccagggtaaa tttcccgtta ttagccagcg catttcgcag cgtttcggtc
     3001 gcttctgcgg cattcagcga accgttagta cggttgttaa cgctatcaac cagcaggacg
     3061 ctacccgcag tcaccccgtc agccccaagc atcttactga ccatcggctg cattgcgcca
     3121 ttccagtcat aatggcgaat atgcggcgca ggcggtgccg ttcgatcttc gtgctcaatt
     3181 gggcctggct gctgcgggat cgtcggcacc gagggcactg tggggacagg ctgttgtggc
     3241 tcggctggtt gttccggcgc tggtttcact tcttctaccg gtgcaggttc acgttgcccc
     3301 acacacccgg cgagaaacat cgccagcgcg gtaatcaagg cgtagcgact catttttgtc
     3361 atcaagattc acccctttac aaatatagat aaagtctgac tttgtgcgcc cccagaaaat
     3421 tggcgctgcc gtacagcgtt accgccgaat gtgcgggaat ggtaacgctg cgtggccttt
     3481 ccagaggatg catctccagc cctctggcgt cataccagta aaaacgataa tgaacggtaa
     3541 cgggttcttg cctttcgtta taaatcgttg aggatgctga aggttgaata tcagacgtcg
     3601 aaaggacggg cttttctgca ctgatgcccg cagccaataa agatgactcc atcaccagcg
     3661 attgctcatc attcaccgga atttccggat gtgaacgaca gcccaccagc agtaacaaca
     3721 ccagagacac cagcccaaag catccttttc tcatcgttac agacctttat gcgccagcat
     3781 tggtcccagc ggacggccac ccaacaagtg catatggatg tggtaaacct cttgtccgcc
     3841 atggcggttg gtattcatga tcagacgata gccatcttcg gcaatacctt cttgctcagc
     3901 aatttttgcc gctacggtga tcatgcgtcc cagcgcctgc tcatgctcag ctgacacgtc
     3961 gttcacagtc ggaatgagaa tattcggaat gatcagaata tgcgttggcg cttgcggcga
     4021 aatatcgcga aacgccgtta ccagatcatc ctggtagacg atatcggagg ggatctcacg
     4081 acgaataatt ttgctgaata tagtttcttc tgccacgaca ttttcctttt tcaaatagcc
     4141 atgcgttgcg ccatgctacg ccgcgccaca ctgcgagtat agagtatggt cgagttaaac
     4201 ctgctctttc aatcttaact cacgatttct taagcaaaaa atcgttttgc tgccgtatat
     4261 atcgccatta ttcccatttc tgcctgcgaa tgcgctgaaa agacattctc tgtttggttt
     4321 acacgacaaa tatgaatgcg tatatttctc atttgcattt gcagcaaaat tattcgcaca
     4381 ctaaatataa ccgctcatca ccctcctccg gcatgagcct gacgacaaca taaaccaaga
     4441 gatttcaaat gctttcaaca caatttaaca gggataatca atatcaagtc atcaccaaac
     4501 cgtcactact tgccggttgc atagcactgg cactattacc ttctgccgct tttgctgcac
     4561 cagccactga agaaacggtg attgttgagg gttcagccac agctccagat gatggcgaaa
     4621 atgattacag cgtaacgtct acctctgcgg gtaccaaaat gcagatgact caacatgata
     4681 ttcctcagtc ggtcactatt gttagccagc agcggatgga agatcagcag ttacaaacgc
     4741 tgggcgaagt gatggaaaac acgctgggga tcagcaaaag tcaggcggat tccgatcgtg
     4801 ctctttatta ttcccgcgga ttccagatcg ataactatat ggttgatggt atccccacct
     4861 attttgaatc gcgctggaat ctgggcgacg cactttctga tatggcactg tttgaacgcg
     4921 tagaagtagt gcgtggcgcg acaggactca tgaccgggac gggtaatcca tctgcggcaa
     4981 ttaatatggt tcgaaaacac gcgacctgtc gtgaatttaa aggcgatgtc tcggcggaat
     5041 acggtagctg gaacaaagaa cggtatgtgg cggatttaca aagcccactc accgaagacg
     5101 gtaaaatccg cgcgcgaatt gtcggcggct accagaataa cgactcatgg ctggaccgct
     5161 acaatagtga aaagaccttc ttctcgggca ttgtcgatgc tgatttaggc gatcttacga
     5221 cgctttcagc cggttacgaa tatcagcgca ttgatgttaa tagcccaacc tggggcggtt
     5281 taccgcgctg gaatactgat ggcagcagca acagttacga tcgcgcacgc agtaccgcac
     5341 ctgactgggc gtacaacgat aaagagatca acaaggtctt tatgaccctg aagcagcggt
     5401 ttgctgatac ctggcaagcg acactgaatg ccacccactc tgaagtcgaa tttgacagca
     5461 aaatgatgta tgtcgatgcc tatgtaaaca aagcggatgg tatgctggtt gggccataca
     5521 gtaattatgg acctggcttt gatcatgtcg gcggcaccgg ttggaacagt ggcaaacgta
     5581 aagttgatgc gctggatttg ttcgctgacg gtagttatga attgtttggt cgtcagcaca
     5641 atctaatgtt tggtggcagt tacagcaaac aaaacaatcg ttcagttcat gggccaacat
     5701 cttcccggat gaaattggca gtttctacaa ctttaatggc aatttcccac aagccgactg
     5761 gtcatcacag agcctggcgc aggacgatac cacacatatg aaatcgttat atgctgccac
     5821 tcgtgtcacc cttgccgatc cgctgcatct gatcctcggc gcacgttata ccaactggcg
     5881 ggttgatacg ctgacttaca gcatggagaa aaaccacacc acgccttacg ctggtctggt
     5941 gtttgacatc aatgacaact ggtcgaccta cgccagctat acctctattt tccagccgca
     6001 aaatgatcgt gacagttcag gcaaatatct ggctccaatc accggtaaca actacgagct
     6061 gggtctgaaa tcggactgga tgaatagccg tctgaccacc acgttagcca tcttccgtat
     6121 tgagcaggat aatgtcgctc agtccaccgg tacacctatc cccggcagca acggcgaaac
     6181 cgcctataaa gcggtggatg ggacagtcag taaaggggtg gaatttgaac tcaacggcgc
     6241 aattaccgac aactggcagc tgacatttgg cgcaacgcgc tatcttgcag aggataacga
     6301 aggaaacgcc gttaatccta atctgccacg caccacggtt aaaatgttca ccagctatcg
     6361 gttgcctgtc atgccagagt tgacagtcgg cggtggtgtt aactggcaaa atcgcgtgta
     6421 taccgacacc gtgacaccgt atggcacctt ccgcgccgag caaggtagct acgcgctggt
     6481 ggatctcttc acccgctacc aggtgacgaa aaacttctcg ttacagggga acgtcaataa
     6541 cctgttcgac aaaacctacg ataccaacat ggaaggttct atcgtctacg gcgcaccgcg
     6601 taatttcagc attaccggca cgtatcaatt ctgaataaca cctgtaaaaa aggcagccat
     6661 ctggctgcct tagtctcccc aacgtcttac ggattagtgg ttacggatgt actcatccat
     6721 ctcggttttc aggttatcgg atttagtacc gaaaatagcc tgaacaccag aaccagcaac
     6781 cactacgccc gctgcgccca gtttcttcag gccagcctga tccactttag acacatcagc
     6841 aacgctgacg cgcagacggg taatacatgc gtcgaggtta gtaatgtttt ctttaccacc
     6901 aaatgcagca accagagccg gtgccatttc gctggtacct gttgcttttg catcttcagt
     6961 cgcgtcttca cgacccggcg ttttcagatc cagtgcttta atcagcacgc ggaagatggt
     7021 gtagtaaaca atcgcataac cgataccgac gatcggtaac agccacagtt tgctgctgtt
     7081 accagacaga acgatgaagt cgatcagacc gtgcgagaac gacgtaccgt cacgcatccc
     7141 cagaagaata cagattggga atgccaggcc tgccagaatc gcgtggatga tgtacaggat
     7201 cggcgcaacg aacatgaaga agaactcgat cggctcggtg ataccggtca ggaacgaggt
     7261 cagcgccgcg gagatcataa taccgcccac tttcgcgcgg ttttctggtt tagcagagtg
     7321 ccagatagca attgcggcag ctggcagacc gtacattttg aacaggaagc caccagacag
     7381 tttacccgca gtcgggtcac ccgccatata acgcggaatg tcgccgtgga aaacctgacc
     7441 tgctgcgttg gtgtattcac caatctgcat ctggaaaggt acgttccaga tgtggtgcag
     7501 accaaacggt accaggcaac gttcgatgaa accgtaaatg ccaaacgcaa ctaccgggtt
     7561 ctggtaagca gcccactgag agaaggtctg gattgcagaa ccaatcggcg gccaaatgaa
     7621 ggacagcaca acgccagtaa agatggcagc caggccagaa atgatcggca caaagcgttt
     7681 accggcaaag aagccaagat actcaggcag cttaatacgg tagaaacggt taaacatgta
     7741 cgctgcaatc gcaccggaga taatccctcc gagtacgcca gtatccgcca agtgtttaga
     7801 ggcgatttct tcagcaggta aatgcagtac cagtggcgca accacggcca tggttttaac
     7861 catgatgcca taggcaacaa ctgcggccag cgcggatacg ccatcgttat tggtaaagcc
     7921 gagggcgaca ccgatcgcaa aaatcagtgg catgtttgca aagacggaac cgcctgcttc
     7981 tgccataaca tgcgatacaa cggcgggcag ccagctgaaa ttcgcggaac cgacgcccag
     8041 cagaatacct gcgataggta gtacggatac cggcagcatc agcgatttac cgaccttttg
     8101 caggttagca aatgcattct taaacataat tgagagtgct cctgagtatg ggtgcttttt
     8161 ttacgttctc acgcgtggca aggggggaga gcctcgccgt gtacagggca tctaagcgcc
     8221 ctttatttat tacacagagt aaaataattc agtgccaatt tgtttgacgg ctatcacgtt
     8281 tcaacttcag atcattggag aagttgcaca ttttgacaaa acatatgtca caatatttca
     8341 aacgcggtta ttcaccgccc aattttcggc ggtgaacttt actcgtttta gccattaatt
     8401 acgagcttta aaaaaactca ttcaacggat ggattgaagg cgggaagcgt cgatgtgaaa
     8461 cagacgggcg aagttatcgg tggttacctg cgccagttct tcaacggcaa cacctttcaa
     8521 cacagccatg tattctgcaa cgtcacgaac catcgcaggt tgattctctt ttcctcgatg
     8581 cggtaccggc gcaaggtaag gtgagtcagt ttccaccagt aatcgatcca gggggacata
     8641 acgcgcagca tcgcgcagtt gctccgcatt acggaaggtc acaatgccgg aaaaggagat
     8701 gtaaaatccg agatccagta atttacccgc cgtttctctg tcctctgtaa aacagtgtag
     8761 tacgccaccg caatccgtca ctttttcttc gcgcagaatt gccagcgtat cggcacgggc
     8821 gtcacgggta tggacgataa ccggcttgtt cagttcacga ccaatctgga tatgatggat
     8881 gaaggactct tgctgacgta ctttagtttc cggtgtgtaa taataatcca gcccggtttc
     8941 acccagcgct acaacaccct cttctgccgc cagacggcgt aaatcttcta catcgtaggg
     9001 atcattctgg ttgagtggat gcacgccaca agaaaatacg acgttgtcac gttcgcctac
     9061 cagatcccgc atatgtaaat aacccggtaa tgttgtggcg actgccagac aaaatttcac
     9121 atcgcgtgcg gcggctttcg ccagaacgtc atccacgtcc ttatgcaaag attcataatc
     9181 cagaccatcg agatggcagt gtgagtcgac taaaaacatg atgtctctct taaagatgag
     9241 gaaccggtaa cacaacgccc ggttgcaggt aatgctcaat acgcagtaaa agatcggtga
     9301 tgagaagctc gcggttgatg cctgtgacag acattaactg ttcacgaatg tggcaaacat
     9361 cccccagtat agcctgcagg cacgagggag aaagatggtt tgccagttcg gcgaccaggc
     9421 ccggcacatc aacattggtc acctgcgcag cgccatgatg gcgttttagc gcatccatca
     9481 gcaacgttgc cagccagtgt aaacgcgccg gagcttgttc atgattaagg gccgccagca
     9541 gcgaatacca gtcgcccgat ggcacgctat atgccaacgc ctgacacaat gtttcacgag
     9601 cctgccagtt atctccctga aacaacgcca gtgccgcgcc aggcgaaccg gcgcttaagc
     9661 gcaatgcggc aagtaatgca tcctgtgaca ttgtcacttc gcgtgaaagc caagtcacgg
     9721 cgtactgttc tggaggcggc gcaaggtaat gtaaccgaca acgactacgt aatgttgcca
     9781 gtaaacgttc aggctcgcgg gtagccagga aaaaccaggt ttctgctggt ggctcttcaa
     9841 gcgttttcag caatgcgtta gccgcggcgt cggtcagtaa ggcagcatcg gttacccaga
     9901 caactttcgc accacctagg cgtgcgtgct cattcagctt ttcggtgacc tcacgtaccg
     9961 catcaatgcc cagcgtattt ttcccttttt cgggagccag ggtgtagtaa tcgggatgcg
    10021 tgccagcctg catcaactga catccacgac agtgaccgca acttttgtgg ccctgcggtt
    10081 gttggcagag taaataacgg ctcagggcgt agattaaagc atcatcgccc atgcccggta
    10141 acgcctgaat gagtagcgca tggtgacctc ttccggcctg atagctggct accagttttt
    10201 cgaaatcagg tcgtaaccat ggataccatc tcatgcgtcc aactccttca cccagtgggt
    10261 cacggtagtg cggattgcat ccatcacggc ctccagcggc tgggtggcat caatggtatg
    10321 aatactttta tcttgtgcag ccagttccag atagcgggcg cgggtgcgat taaagaaatc
    10381 gaaagactct tgctcaatac gatccagctc gccgcgcgca cgcgcgcgtt ttaagccaac
    10441 ttccggggta acatcgagat agagcgttaa gtctgggcga aaatccccga gaacagcatc
    10501 acgcagtgtt gccagcatat gttggtcaat accacgtcct ccgccctgat acgcctgagt
    10561 ggaaagatcg tggcgatcgc caatcaccca ggtgccgtta gccagcgcag gtttgatgac
    10621 cgtttctacc agttgaacgc gcgcggcata aaacatcaga acttcggctt tatcggtaat
    10681 gacttcatcg cctaccgatt tgatatccag caccaggctt cttaactttt cggcaagttg
    10741 cgtaccgcca ggttcccgag tgaaaaccat gtcgcggata cccagttgct cgagcgtctc
    10801 aaccaccaca ttacgcgcgg tagttttgcc tgcgccttcc agcccctcaa tgacgatata
    10861 cttactgcgc atttttttcc ttaagcactt tcagataatc ctgcacagac ttgttatgac
    10921 tggcaagatt ggtattaaac gtgtgaccac ctttaccatc ggccacaaaa tagagatacg
    10981 gcgtttttgc cggatgcgca gcagccttca gcgaatccgc ccccggcgta gctatcgcac
    11041 ccggcggcaa accggtaatg gtataggtgt tatacgctgt cggcgtttcc aggtctgcac
    11101 gagaaagttt gccattataa cgctctccca tcccgtaaat cacggttggg tcggtctgca
    11161 ggcgcatacc aatgcgtaaa cggttgataa ataccgaggc aacctgatcg cgttcactgg
    11221 caacggcggt ttctttttcg ataattgatg ccatcgtcac cagctgattt ttatctttat
    11281 aaggcagacc gtccgcacgc ccttcccagg cgctatcgac cgctttcacc attttcttgt
    11341 gaactcgctt gagtaacgcg acatcggtgg tattggcggt atacatcaag gtgtctggcc
    11401 agaaccaacc ttcaatccac tccgggtttt ccagttcaag tgcctgcgct acggtggcgt
    11461 acttatcgtc actcagcgtg tgcttgatat acggagcctc acgcaattgc ttgaggtagt
    11521 cgctcagacg catcccttct accagtcgca gcgggaactg tgcttcttta ccgctttcca
    11581 acaatttcag catctcgcgc acggtcatct gcggtgtaaa gcggtaagtc ccggctttaa
    11641 agtgagaaag atccggttcg atacgcagca gccattgaaa aacccgtggg cgattgatga
    11701 tcttatcagc ataaagctgt tcaccgagcg ccagacgtcc ggtccctggc ttcagggtaa
    11761 atatcgtctc ttctttgata agcaatttgc tgtcggcaag atggcgaacc ttccagacgc
    11821 ccacaccagc ggcgataccc agtaccacca gcaataacaa gataatcaat aacacttttt
    11881 tcatgactaa ttcgggcgct cacaaagtgg ggctaaatat tcatataacg ttgctgacga
    11941 aaaggtgaca tcgccacagg cacgtacggg catcactggc attaacgcat tacaaataac
    12001 catctcatct gcctgcaacg cctcctccag cgaggcttgc acttcgacaa gctgataaga
    12061 ggattgtgcc agtaaacgga tacagaattg tcgcataatg ccgttaacac ctgcctgatc
    12121 cagtcgcggc gtataaacta cattgccctt ccgccagaac aaattagccg cacagcattc
    12181 cgtaacccac ccttcgctgt caaggaccag cgcctcatca gcgtttgtct gctcaagatg
    12241 agagcgaatc aatacttgct caagccgatt aagatgttta atacctgcaa gatgaggatt
    12301 gcgccccagc cgcaccgggc ttagcgccaa cgtcatccct tcgttacgca aacggtcgta
    12361 atgtgcagga taagccgtaa cggagagaat ccgcgttgct ggtccgctgt tcaatgtgct
    12421 gtaccctcgc ccgccactac cgcgactgat cacgactttc agcacaccat tttgctgttc
    12481 tgctgccagc gttttcatct cttgttcaag ctgaggccag aagtcacagg aaatcatcaa
    12541 ccgctgacaa gcatcctgta gtcgcaggat atgcgccgat aacaaactga ctttaccgtc
    12601 gataactctg gcggtggtaa aacaaccatc accaaactgc gttgcccgat cgcttactgc
    12661 cagcgattcc tgcttataac cgttaattaa gaacataccg gctccttatc agagaattgt
    12721 tagtgtggca gcatgttcac tacggaacaa gtcggaataa aagctaagaa aaaaggcccg
    12781 caagcggacc ttttatatgg gtggaaaatg acaacttaga tctttttaaa gatcaaagaa
    12841 ccattagtgc caccgaagcc gaaggagtta cacagcgtgt attccattcc gctgacctga
    12901 cgtgcttcat gcggtacgaa atccagatcg caaccttcat ccgggttatc caggttgatg
    12961 gttggcggaa cagcctgatc gcgcagcgcc aggatggagt agatagattc tactgcaccc
    13021 gccgcaccta acaggtgacc ggtcatagat ttcgtggagc ttaccaacac acggcttgca
    13081 gcttcaccga agatggtttt caccgcctgc gcttcagctt tatcgccagc cggcgtagaa
    13141 gtaccgtgcg cgttaacgta gccaatctga ctcgcttcaa tgcctgcatc acgcagagca
    13201 tttgccattg ccagagctgc gcctgcgcca ttttctggcg gtgacgtcat atgataagca
    13261 tcgctgctca taccaaagcc gacgagttca gcgtaaattt tcgcaccgcg ttttttcgcg
    13321 tgttcgtact cttcaagtac cagcataccg gcaccatcgc ccagtacgaa gccatcacgc
    13381 tctttatccc acgggcggct cgccgcttgc gggttatcat tgcgggtaga taatgcacgt
    13441 gccgcgccaa aaccaccaac gcccagcggc gtactggctt tctctgcgcc acctgcaacc
    13501 atcacgtcag catcgccata cgcgataata cgcgcagcat ggccaatgtt gtgcacgccg
    13561 gaagtacagg cggtcgcgat agagatgctt gggccacgca ggccatacat gatagtcaga
    13621 tgacctgcca ccatgttcac aatcgttgac ggaacgaaga atgggctgat cttacgtgga
    13681 ccaccgttca tcagagatgt gtggttttct tcgatcagtc cgaggccgcc aatcccggag
    13741 ccaattgcgg caccaatgcg ggttgcgttc tcttccgtta tttcaaggcc agaatcctgc
    13801 atggcctgaa cgccagcgac aattccatat tgaatgaagg catccatctt gcgctgttct
    13861 ttgcgcgaga taatgtcctc acagttaaaa tcctttacta agccagcaaa tttcgttgca
    13921 taggcgctag tatcgaaatg gtcgattagg ctgatgccac tctgaccggc aagcagagct
    13981 ttccaggtag actctacggt attgccgaca ggagacaaca tgcccagtcc ggtcacaact
    14041 acacgacgct tagacacgtt tgtcctccaa ggagggaaaa atgattctag tgggacaaaa
    14101 agataaaact caggcggtcg aacgaccgcc tggagatgtt cacttacgcc tggtggccgt
    14161 tgatgtaatc aatggcagcc tgaacggtgg tgattttctc agcttcttcg tccggaatct
    14221 cagtatcaaa ctcttcttcc agagccatta ccagctcaac ggtgtcaaga gaatccgcgc
    14281 ccaggtcttc aacgaaagaa gcattgttgg taacttcttc ctgcttaacg cccagctgtt
    14341 cgccgataat tttcttaacg cgttcttcga tagtgctcat actcttaaat ttcctatcaa
    14401 aactcgcttt cgcgatggtt ttcgtagtgt ataaaatgtt gaaaaatttg caactaaatc
    14461 ccggcaggtc ttaccacgat tttacgttat tttgaggcct taccccccaa taacgcaaat
    14521 cattttgcac taattattac tcataaccac gcagactgcg cgcaacatga gcattttgtg
    14581 caaatcgcgg tcagaccatg tacatcccgc cgttcacatg caaagtttca cccgtgatgt
    14641 aagctgcttc gtcggatgcc aggaatgcaa ccgcgttggc gatttcctgt gcgccgccga
    14701 ggcgacccgc aggaacctgc gccaggatac ccgcacgctg gtcatcgctc agcgcacgtg
    14761 tcatgtccgt ttcaataaag cccggagcaa caacgtttac agtaataccg cgtgacgcaa
    14821 cttcgcgcgc cagtgattta ctgaagccga tcaagcccgc tttcgccgca gcgtagttgg
    14881 cctgaccgcc atttcccatg gtaccaacca cagaaccgat agtgataata cgaccatgac
    14941 gctttttcat catagcgcgc attaccgctt ttgacagacg gaaaacagat gaaaggttag
    15001 tttcgataat atcgttccac tcttcatctt tcattcgcat taacaggtta tcacgagtga
    15061 taccggcatt attgaccagg atatccactt caccaaattc tgcgcgaatt ttttccagaa
    15121 cagattcgat agatgccggg tcggtcacat tcaacatcag acctttgccg ttggcaccta
    15181 aataatcact gatcgcctga gcgccatttt cactggtcgc agtgccaata actttcgcgc
    15241 cacgggctgc gagcgtttca gcaattgcgc ggccaattcc gcggcttgca ccggttacca
    15301 gtgcgatttt tccttcaaaa ttcatgattt tcctctttta aagctcgagc gccgctgcca
    15361 tcgctgaagg ttcgttcagc gccgaggcgg tcagggtgtc gacaatgcgt ttcgtcaggc
    15421 cagtaagcac cttgcccggg ccgacttcat agagatgttc tacgccttgc gctgccatgt
    15481 actcaacaga cttcgtccac tgaaccgggt tatacaactg acgtaccagt gcgtcacgga
    15541 tggcatcacc attggtttcg catttcacat caacgttatt cacaacagaa actgttggtg
    15601 cgttaaaggt gattttcgct aattctactg ccagtttgtc ggctgctggt ttcatcagcg
    15661 cacagtgaga cggtacgctc actggtaacg gcagcgcgcg ttttgcgcca gcagctttac
    15721 aggcagcgcc agcacgctca accgcttctt tatgaccggc aataaccacc tgtcccggag
    15781 agttaaagtt taccggagaa acgacctgac cttctgcagc ttcttcacac gctttcgcaa
    15841 taggcgcatc atccagaccg atgattgccg ccatagcgcc cgtgccttcc ggtacggctt
    15901 cttgcatgaa cttaccgcgc atctcaacca gacgcaccgc atcagcgaaa tcaatcacgc
    15961 cagcgcaaac cagcgcggag tattccccca ggctgtgacc ggccatcatt gccggtgctt
    16021 taccgccctg ctgctgccat acgcgataca gcgcaacaga tgcagtcaac agcgctggct
    16081 gggtttgcca ggttttattc agttcttcag ctgacccctg ctgggtcagc gcccacaggt
    16141 cgtagcccag cgccgcagaa gcttcagcaa acgtttcttc gacaattgga tagctcgccg
    16201 ccatatcagc cagcattcca acggtttgag aaccctgtcc agggaacaca aatgcaaatt
    16261 gcgtcatgtt ttaatcctta tcctagaaac gaaccagcgc agagccccaa gtgaatccac
    16321 cgccaaaggc ttcaagcaga accaactgcc ccggcttaat gcgcccgtcg cgtacagctt
    16381 catccagcgc gcacgggaca gaggccgcag aggtattacc gtggcgatcc agcgtcacca
    16441 cgacattgtc catcgacata ccgagttttt tcgccgttgc actgataata cgcaggttag
    16501 cctgatgcgg aaccagccag tccagttgag aacggtcaag attattcgcc gccagcgtct
    16561 catcaacgat gtgcgccagt tccgttaccg caaccttgaa gacttcgttg cccgccatcg
    16621 tcagatgaat tgaattctct ggattcacgc ggtcggcgtt tggcagcgtc agcaattcac
    16681 cataactacc gtcggcatgc agatgggtgg aaatgattcc cggctcttca gaggcagcca
    16741 gcaccgcagc gcccgcgcca tcgccaaaaa taataatagt cccacgatct gttggatcgc
    16801 aggtgcgcgc cagtacatcg gaaccgacga ccagagcata cttcaccgcc ccagatttca
    16861 cgtattgatc ggctacgctt aatgcatagg tgaaacctgc acaggctgct gcaacgtcaa
    16921 atgccgggca acctttaatg tccaacatgc tttgaatctg acaagctgcg ctcgggaaag
    16981 cgtgcgtagc agaagtcgtt gccacaacga tcaggccaat ctggtctttc tcaatgcccg
    17041 ccatctcaat tgcgcgtgtc gccgcttcaa agcccatggt tgaaacggtt tcgtttggcg
    17101 cggcaatgtg gcgttcgcgg ataccggtac gagtgacaat ccactcgtca gaggtgtcca
    17161 ccattttttc caaatcggcg tttgtccgca cttgttcggg cagatagctg ccagtaccaa
    17221 taatcttcgt atacatgtac gctcagtcac ttttcggtta tataccgtca cttgcaaact
    17281 gcgagttcgc tggcagcgtc ctgctaccgc agagttccgc ttttgccacc gtccagcagc
    17341 tcaaaaccag ctgggtatac agattccagg cgagcggcaa ttcgctgagg aacttgtcgc
    17401 tgcaccgcct gcactgcctg ttcaatcgcg accgcaaaag ctcgctgatt ggctgcacca
    17461 tgacttttta tcaccgtgcc gcgcaatcct aacagacagg cgccgttata ctggtcgggg
    17521 ttgaggtgac tgaatcgcct cgtcaggctc ttttgtagcc aacgctttaa taacagtagc
    17581 caccacgacc gttttttccc ttcaccctga gatttcagca gagaaaggaa catcctgaca
    17641 acaccttcca tcgtctttaa cgtgacattt cctgtaaagc cgtcacaaac cagcacatct
    17701 gtcttgccag ttaacaactc attggcttca agatagccga tataattgat agaagggatt
    17761 gttttaagca ccgctgaggc atcccgaata ctgtcaagac cctttacttc ttcttcacca
    17821 atattgagca acgccacgcg aggattggga atttccacca cctcttcagc cagaactgag
    17881 cccataatgg caaattgcac caacattgtg ctgtcacaat cgacgttggc ccctaaatcg
    17941 aggaccaccg ttttgccctt ttgctgatgt ggtaataccg tcaccagcgc cggacgctca
    18001 atcccctcca ggggcttgag taataatttt gccagcccca tcagtgcccc ggtattaccg
    18061 gcactgacac aggcttgcgc tcgaccttct ttcaccagct ccagggccat acgcattgaa
    18121 ctcccacggc tggcgcggat agcttgcgaa ggccgggcat cactggcgat aactgactgc
    18181 gcaggaataa tctgcagacg cgaacgttgt tcaaagtcag ctttagcaag taatggcgtg
    18241 atggcgtcgg gattgccgac taaaagaaga gtgagttgcg aattagaatt cagtgcctgc
    18301 aatgctgcag gcactgtcac ggaagggcca aaatcccctc ccatgacatc taacgccagg
    18361 gttagacgtg tcaaggtatc gtcgccgccc ggtttggtct ttccccagtt gcctggggaa
    18421 aatcctcact aagcttcatc acgcagacgc gtgattactt agcgatgacc ttgcggccgc
    18481 ggtagtaacc gtcggcagtg atgtggtgac gcaggtgttt ttcaccagaa gttttgtcta
    18541 cagacaggct ggtgactgcg gtcagcgcgt catgggaacg acgcatgcca cgtttggaac
    18601 gggttggttt attctgttgt acggccatgg accttactcc tcaattacgg tttatccccg
    18661 atccaacggg gagcaccaat tacttacgct ttaagctggc taatacggca aatgggtttg
    18721 gcttttgcac ttcttcaggc agttcaccaa agaccatgtc cgcttcggac acttcacagt
    18781 gttcagaatc atgcaccgga actaccggca aggcgaggat gatttcatct tcaaccattg
    18841 caagcagatc gatttcaccg aattcgttaa cctcaatcgg ttcatacgct tccggcagtg
    18901 cttcagcctg ttcgtctgaa cgcacaggac taaaacaata cgttgtgtag acctgatgag
    18961 taaacggctt cccgcaacgc tgacactcga gcgttaccgt caccttcgca tcgccgttta
    19021 acactgcgag acgttggtta tcgatagcga acgacatgga gcattccaca tcactgtcca
    19081 cactgactac ggattcggcg acgcgctcaa cctgatcagg ggtatagata ccctggtaat
    19141 caaggcgttt ttgagccgta cgaaccggat cgagagtcag gggtaatttt accttttgca
    19201 tagggcgcgc atattaactt tgtaacgtca tagagtcaaa gaaaaaggca gcctgtggtt
    19261 gccttttgcc aataattcgc acacattgcg ggttactgct ttattttcgt tcagtgctga
    19321 taacgaacat caacctgcaa aacgaagcgc atagtttaaa atggctatca tcaatacgct
    19381 atatctgatg gaaaaaaata tgcctaaact tattttagcc tccacctcgc catggcgtcg
    19441 cgctcttctg gaaaaactgc aaatctcttt tgaatgtgca gcacctgagg tcgacgaaac
    19501 cccacgcagc gacgaatcac cgcgacagtt ggtgcttcga ctggcacaag aaaaatcgca
    19561 atctctggcg tcacgttatc cggatcattt aattattggt tcagaccagg tatgtgttct
    19621 tgatggcgaa atcaccggta aaccgttaac ggaagaaaat gcccgtctgc aattacgcaa
    19681 agccagcggc aatatcgtca ccttctatac tggactggcg ttgtttaatt cggcgaatgg
    19741 gcatctacaa acagaagtgg agccttttga cgtccatttc cgtcacctaa gcgaggcgga
    19801 gattgataat tacgtgcgta aggagcatcc cctgcactgc gcgggtagct ttaagagtga
    19861 aggatttggc attacgctgt ttgagcgttt agagggacgt gaccctaaca cgctggttgg
    19921 tttaccgcta atcgcgctgt gtcagatgtt acgtcgggaa gggaaaaacc cgctgatggg
    19981 atagttgttt gcgccggatg cggttacgtc gcatccggca atcggtgcag gcggcctgat
    20041 gcggcgtaaa cgccttatca ggccaaccgc ccattatcag gcttatatgc ttgtattagc
    20101 gcgcgttacg cagcttttgc aaacaacgct tcaaaccttc atccatcggc gcttcgatac
    20161 gcatcacctc accggtcccc ggatgggtaa acttcaacgc tgcggcgtgc aggaacagac
    20221 gatttaatcc cgtgcctgct tcagtgagct gtctgtcaaa ttcacggtca ccgtagcgat
    20281 cgtcaaaggc aatcggatga cccgcatact gtgtatgcac acggatctga tgagtacgtc
    20341 cggttaccgg gctacaacgc accagggtgg caaatgcata gcgttcttcc actttaaagc
    20401 gtgtttccga cggtttgcct tcctgactga cacgcacgat acgttcgccg ctttgcagaa
    20461 tatttttcaa taacggcgct tgaacgctct tcacatgcga ctgccactga ccgcgcacca
    20521 gcgccaggta atctttttgc attccttttt cacgtaattg ctcatgcaga gaacgcaacg
    20581 ccgagcgttt tttcgctacc agcaacacac ctgaagtgtc ccggtcgaga cgatgaacca
    20641 gttcaaggaa ccgcgcttcc gggcgcaacg cccgcaaacc ttcgataacg ccgaagctta
    20701 aaccactgcc gccatgtacc gccgtaccgg aaggtttatt cagcaccagg atatgatcat
    20761 cttcatataa gatgacgtcc gccagcgccg ccaccttttg cagatgtggc gaaaccgcct
    20821 cttcttcccg ctcagcaacg cgaaccggtg ggatacgcac ctcatcaccc gcttcgagtt
    20881 tatattcagg cttaatacgt tttttgttca cccgcacttc acctttacgc aaaatacggt
    20941 aaatcatact ttttggtacg cctttcaatt gggtacgcaa aaagttatcg atacgttgcc
    21001 ccgcttcgtc agcggtgata gcaacaattt ttacggatgg agtctctgtt ttcatggtgg
    21061 gcgattctaa ttagccaaca ggattcgcgc cactcatttt tctatgctta tttttagttt
    21121 tgcaccttat tacttcacta cgtgatcact ttattgatgg ttattaaacc aatcaccagc
    21181 aagaagtgaa aaaactgtga gtaagcgggt gataaatggt aaaagtcatc ttgctataac
    21241 aaggcttgca gtggaataat gaggccgttt ccgtgtccat ccttgttaaa acaagaaatt
    21301 ttacggaata acccattttg cccgaccgat catccacgca gcaatggcgt aagacgtatt
    21361 gatctttcag gcagttagcg ggctgcgggt tgcagtcctt accggtagat ggaaatattt
    21421 ctggagagta atacccagtc tgtttctttg ataattgcgc tgtttttccg catgaaaaac
    21481 gggcaaccga cactctgcgc ctctttgagc tgacgataac cgtgaggttg gcgacgcgac
    21541 tagacacgag gccatcggtt cacacccgga aaggcgttac tttgcccgca gcttagtcgt
    21601 caatgtaaga ataatgagta agttacgatg aaaagaatgt taatcaacgc aactcagcag
    21661 gaagagttgc gcgttgccct tgtagatggg cagcgtctgt atgacctgga tatcgaaagt
    21721 ccagggcacg agcagaaaaa ggcaaacatc tacaaaggta aaatcacccg cattgaaccg
    21781 agtctggaag ctgcttttgt tgattacggc gctgaacgtc acggtttcct cccactaaaa
    21841 gaaattgccc gcgaatattt ccctgctaac tacagtgctc atggtcgtcc caacattaaa
    21901 gatgtgttgc gtgaaggtca ggaagtcatt gttcagatcg ataaagaaga gcgcggcaac
    21961 aaaggcgcgg cattaaccac ctttatcagt ctggcgggta gctatctggt tctgatgcca
    22021 aataacccgc gcgcaggtgg catttctcgc cgtatcgaag gcgatgaccg taccgaatta
    22081 aaagaagcac tggcaagcct tgaactgccg gaaggcatgg ggcttatcgt gcgcaccgct
    22141 ggcgtaggca aatctgctga ggcgctgcaa tgggatttaa gcttccgcct gaaacactgg
    22201 gaagccatca aaaaagccgc tgaaagccgc ccggctccgt tcctgattca tcaggagagc
    22261 aacgtaatcg ttcgcgcctt ccgcgattat ttacgtcagg atatcggcga aatccttatc
    22321 gataacccga aagtcctcga actggcacgt cagcatatcg ctgcattagg tcgcccggat
    22381 ttcagcagca aaatcaaact gtacaccggc gagatcccgc tgttcagcca ctaccagatc
    22441 gagtcgcaga tcgagtccgc cttccagcgt gaagtccgtc tgccatccgg tggttccatt
    22501 gttatcgaca gcaccgaagc gttaacagcc atcgacatca actccgcacg cgcgacccgc
    22561 ggcggcgata tcgaagaaac tgcgtttaac accaacctcg aagccgccga tgagattgct
    22621 cgtcagctgc gcctgcgtga cctcggcggc ctgattgtta tcgacttcat cgacatgacg
    22681 ccagtacgcc accagcgtgc ggtagaaaac cgtctgcgtg aagcggtgcg tcaggaccgt
    22741 gcgcgtattc aaatcagcca tatttctcgc tttggcctgc tggaaatgtc ccgccagcgc
    22801 ctgagcccat cactgggtga atccagccat cacgtctgcc cgcgctgctc cggtaccggt
    22861 accgtgcgtg acaacgaatc gctgtcgctc tctattctgc gtttgatcga agaagaagcg
    22921 ctgaaagaga acacccagga agttcacgcc attgttcctg tgccaatcgc ttcttatctg
    22981 ctgaatgaaa aacgttctgc ggtgaatgcg attgaaacgc gtcaggacgg tgttcgctgc
    23041 gtgattgtgc caaacgatca gatggaaacc ccgcactacc acgtgttgcg cgtgcgcaaa
    23101 ggggaagaaa cgccgacctt aagctacatg ctgccgaagc tgcatgaaga agcgatggcg
    23161 ctgccgtctg aagaagagtt cgctgaacgt aagcgtcctg aacaacctgc actggcaacc
    23221 tttgccatgc cggatgtacc gccagcgcca accccagctg aacctgccgc gactgtcgta
    23281 gccccagcac cgaaagctgc aacggcaaca ccagcagctc ctgcacaacc tgggctgtta
    23341 agccgcttct tcggcgcact gaaagcgctg ttcagcggtg gtgaagaagc caaaccgacc
    23401 gagcaaccaa caccgaaagc agaagcgaaa ccggaacgtc aacaggatcg tcgcaaacct
    23461 cgtcagagca accgccgtga ccgtaatgag cgccgcgaca cccgtagtga acgtactgaa
    23521 ggcagcgata atcgcgaaga aaaccgtcgt aatcgtcgcc aggcacagca gcaaactgcc
    23581 gagacgcgtg agagccgtca gcaggctgag gtaacggaaa aagcacgtac caccgacgag
    23641 cagcaagtgc cgcgtcgtga acgtagccgc cgccgtaacg atgataaacg tcaggcgcaa
    23701 caagaagcga aggcgctgaa tgttgaagag caatctgttc aggaaaccga acaggaagaa
    23761 cgtgtacgtc cggttcagcc gcgtcgtaaa cagcgtcagc tcaatcagaa agtgcgttac
    23821 gagcaaagcg tagccgaaga agcggtagtc gcaccggtgg ttgaagaaac tgccgctgcc
    23881 gaaccaattg ttcaggaagc tccagctcca cgcacagaac tggtgaaagt cccgctgcca
    23941 gtcgtagcgc aaactgcacc agaacagcaa gaagagaaca atgctgataa ccgtgacaac
    24001 ggtggcatgc cgcgtcgttc tcgccgctcg cctcgtcacc tgcgcgtgag tggtcagcgt
    24061 cgtcgtcgct atcgtgacga gcgttatcca acccagtcgc caatgccgtt gaccgtagcg
    24121 tgcgcgtctc cggaactggc ctctggcaaa gtctggatcc gctatccaat tgtacgtccg
    24181 caagatgtac aagttgaaga gcagcgcgaa caggaagaag tacaagtgca gccgatggtg
    24241 actgaggtcc ctgtcgccgc cgctgtcgaa cctgttgtta gcgcgccagt tgttgaagaa
    24301 atggctgaag tcgtagaagc ccccgtaccg gttgccgaac cgcaaccgga agtggttgaa
    24361 acgacgcatc ctgaagtgat tgccgccgcg gtaactgaac agccgcaggt gattaccgag
    24421 tctgatgttg ccgtagccca ggaagtcgca gaacacgcag aaccgatggt tgaaccgcag
    24481 gaagagacgg cagatgcaga tattgaagaa gttgccgaaa ctgctgaggt tgtggttgct
    24541 gaacctgaag ttgttgctca acctgccgcg ccagtcgtcg ctgaagtcgc agcagaagtt
    24601 gaaacggtgg ccgcggttga accagagatc accgttgagc ataaccacgc taccgcgcca
    24661 atgacgcgcg ctccggcacc ggaatatgtt ccggaggcac cgcgtcacag tgactggcag
    24721 cgccctactt ttgccttcga aggtaaaggt gccgcaggtg gtcatacggc aacgcatcat
    24781 gcctctgccg ctcctgcgcg tccgcaacct gttgagtaat aattagctca aagtaatcaa
    24841 gccctggtaa ctgccagggc ttttttattt catgtttgaa tcaacatatt aaaattcatc
    24901 aaatggcatc cttgctaacc aacaatgcaa aataggcaaa aaaaatgccc gccccggcga
    24961 tcccagagcg ggcagacaaa acatacccag tttcatgata tgttttaaag cgagcttatt
    25021 tgttgagctg gaagagcgac aatccctgca tatcggtaaa tgctttatac gatgcctgca
    25081 atgccgtttg ctgcatgatg taagatgaaa tagttgcatt ccagtccaca tcaaccagat
    25141 cgctcatctg ctgcgtttgc cctaaagcgc gatcgctacc taaggaatcc agcgactcca
    25201 gtttgttcag ctgcgtgcct aattccgcgc gaacggtaag gacattattc agcgagtttt
    25261 tcagtccacg gttggtttta tccaatgccg cagcggcgat ttctttatcc gcttcgctat
    25321 ccgcgaccgg cgttttcagg gctgcgatgg cgctatccag catggcaaaa agattggttt
    25381 cagaagcgct accgtctggt tccgctaccg cgttactggt aatactgtcg aaaattttgt
    25441 cgcccgtatg tcctatcacc atcgaacgcg aagcatcgac ctgttgctta atactttctg
    25501 ttccgccaac ataatcaccg ttagcctcac taaacggcgc agtctctgtt ttatatgtag
    25561 cgcccattac cgtcagtggt gttcgccaga ttcagcaact ggtcacgaag cccctgaata
    25621 tccgtagcca gcgaggcccg gtcatcgtca ctcaaggtgc cattgctggc gtagacaatt
    25681 ttttcctgag cattctggat agcagtggtg acctggctaa gtacactctc ttccagtgac
    25741 actttttgag tggcgaaagt acgtgccagc gtgtactggc tgttttgcgc ctgtgcctgg
    25801 gagagaacaa cagcttgtga tgcagcaatg ggatcgtctg aagggttaac gactcgctta
    25861 cccgtcgaca tctgctcgcc gtacttcatc cattctgcct gagaattggt gatgccgcgc
    25921 atgttttgct ggtacatcat ctgggtactg aaacgcattt tatctcccct tagcgaatgt
    25981 taatcagcgc atcaaaaatc gcgtttgccg tctgcagaac ctgcgcattc gccaggtaat
    26041 actgctgaaa acgttgcaga tttccgtact cctcatcgag attgacaccg gaaatcgact
    26101 gctgctgatt ggaaagctgc gtcaccacat taccttgcgt ggtgctactg gttttcaacg
    26161 tcgcggtttt attaccgata tcactcacta acgaggcata agcgtcgtta aaggatttcg
    26221 caccgcccac cgttttactg ttgctttgca gatccagcag ggcctgaccg ttgcggttgt
    26281 cgctatcacc cgcatcttct tcgctcgcca tcgcgatttt cgcttcgtcg gtgattaata
    26341 catccatgtt gacgatggcg tcacttactg gtttcagcgt gaagctgtcg ttaacggcag
    26401 gcgttcctgt aaacgtcaac tccagaccat caaatgccac tttaccgttg gcatctggtg
    26461 tcgccgtaaa agtggtattg ctggcaaggc gggtgacctg ccactgatta ttatcgaacg
    26521 agattttgta atctgtcgcc agtaccacgg aggcatcagt taccgtggca ccgatcgcaa
    26581 cgtcaccttt gtttttcgtg ttttgcagaa ccgcgggctt accgatagta aagaaatctt
    26641 caccagcatc gccgttagca tcaaatccgg ctttgtgttg gctgttgaaa gcctcggcaa
    26701 atgccagcgc cagttgtcca agcgtattac gcgtctggtc cagatcctga gaacggaatg
    26761 tcagaatgcc gcccagcgac ccggtattca gtaatttctc cgggatctca atattgcctg
    26821 ccgtcccatc aacgtaagcg acagtcgtac gagaagggtc ggcgctggaa ggaactgccg
    26881 ccagttgccg cgccgtactt ccctgaacca gtgagtaacc attggcatcg tgatgttata
    26941 agtaccgcca tcctgaacgc tgacttctac gccaacaatc tggtttaatt cgctcaccag
    27001 ttgatcgcgt tgatccagca gattgttagg tgacgcccct gcccccacgc ctgtcaggcg
    27061 cgagatttga tcgttcaggc tggcaatttg tttagcgtaa ttgttgatct gatcaacgct
    27121 ggcacctatc gcgatattga cctgtttgtc ctggtcgcgc agatattgat cggtggtttt
    27181 aaactgattc accaaccctt ctgatttccc aatcagcgcc tggcgcgccg ccgggtcttc
    27241 cgcgttactc accagcgttt gcaggctggt gaagaaatcc tgcatctgtg ttgccagcga
    27301 agaggtactg gtggagagca tattgtcgat tttcgacatc tgctcatagc gggcggtcag
    27361 gccgctactt tgcgtctgcg cagcacgtaa ctggttggta ataaacgtat catactcacg
    27421 ctgcacacca gaaacgtaga cgccattgcc aacccagccg ccagcgccca acgtgctatt
    27481 ggcctgcgcc ataatagtgg tttggcgggt atatccggca acgttatagc tggagatatt
    27541 attacttgcc gtatttaacg ccgcctgggc cgcgttcagt ccgctcatgg cgttattgat
    27601 caagctggac atggaggttc cttataagcc ttcaattaca gagtattatc ggcagcgacc
    27661 cgccggactt gagttattca gaacagatta tcaatgttca tgctgtaggt tttgctcacc
    27721 ttgtcgctta tcgatttcat ctgctggatc atgttggtga gtttacgggc atagtgagga
    27781 tcggtagcat agcccgcgtc ctgtaggacc tgcgccccct gttccgcact cgcggcggtc
    27841 gtcacggcgg cgtagcgcgg gttacgtgtt aacagcccaa cgtaatccga caatgcttcc
    27901 agatacgagc tgtagacccg aaacttcgct tttactttct tcgcttcgcc gttttcatat
    27961 tcagtcgtgg tgatttcagt gacttgccct ttccagttgc cagaggcttt gacaccaaac
    28021 aggttatagc ttggctcgcc gttttcgcgg cggatttgcc gttgccccca accagattca
    28081 agcgcagcct gagcgaggat caaatgatgt ggcacaccgc tttgctggct tgccagttgg
    28141 gcgggcagcg agagttgcgc gaggaatgct ctactgtcac ccggcagcga atcatcgtag
    28201 ttacgtggta cggccttttg caccagctgc gaaagcgtct ggttttgata acgcaccacg
    28261 gtttcgagcg ggaatttcat cggtgctgct ggcgtggact cctctggcaa tggttgttct
    28321 ggcgtcatct gtttaaccat catctctgca agccccagac ctttgcccgc cgtcatctgt
    28381 tgggcaattt gctggtcata catactggta tacagacgag tgtgctcgct gctgaacagg
    28441 ccatcttttg gtaaagcgtc gcgcatgctt ttcaacatca tctgcacgaa catcccttcc
    28501 acctgacggg ccaccggacg gatatttgcc gccggatctt cgctcgcttt cgcctttagt
    28561 tcgttgagtg attgcgcatc ccaggccgca cttgccagta gtttgctgtc gctgatcatc
    28621 agatgatttc cagttttgcc cgcagacatc ccgcactttg cattgattgc aaaatagaca
    28681 tcagatccat cggcgtagcg cccagcgcat tgagcgcacg caccacgtta ttgaggctgg
    28741 cgctggaacg tacgctttgc agcgaaccgc cgctctggcg taaatcgatc tgcgtttgtg
    28801 gagtaacaac agtctgtcca ccaccaaacg gtgtatctgg ctggctgaca ttggcctgac
    28861 gattaactgt tactgagaga tttccctgcg ctaccgcgca gctgtcgagg gtcacttcgc
    28921 gattcatcac caccgaaccg gtgcgcgagt taatcactac tttagcgtcc tgcggggtga
    28981 cattaacctg catattctgg atatcggcaa ggaaacggac ctgggaactg ttgccacttg
    29041 gtacgcgcac ctgaatagtc cgcgcatcta acgcggtggc gctgccatat ccacgcacgc
    29101 ggttgatggt gtcagcgatt tgctgcgcca tgctgaaatc ttcgtcgtta agttgcaaat
    29161 taagggtatt cccgacgcca aactggctgg gcaattcacg ttcaataacc gcaccattgg
    29221 tgatccgtcc accgttcagt tggttaacct gaacactgct accgcctgcg gaggctcctg
    29281 cgccgccaac cagaatattg ccctgcgcca gcgcatacac ctgactgtca acgcccttaa
    29341 gcggagtcat caacaacgta cctccacgca agcttttggc attacccatg gaagaaacca
    29401 ccacatcgat ggtttgcccc tgacgtccaa acggaggaag tgacgctgtc accattaccg
    29461 cagcgacgtt ttttagctgc atattggtgc ccgtcggaac ggtaattccc agctgtgaga
    29521 gcatgttatt aagcgtttgt gtggtaaacg gcgtctgggt tgtctggtca ccagtgccat
    29581 ccagccccac taccaagcca tagccaatca gtgagttttg ccttaccccc tgaacactgg
    29641 tgagatcgcg aatacgctca gcctgagccg ccgtcgtaac cagtagaaga attaatgcag
    29701 agagaaattt aatcaccaca gcctcactta cattggcgac aggttaagga agaaacgctg
    29761 caaccagccc atattttgcg cttcgttaat gtagccattg cctacgtatt caatacgcgc
    29821 atccgccacc tgagtagacg gtacggtatt gctgccgctg atagtgcgtg gattaaccac
    29881 gcccgagaag cgaataaatt cggtaccctg attaatggca atctgttttt cacccaccac
    29941 atgcaggttg ccgttgacca gtacctggtc aaccgtcacc gtcaacgtgc cgctaaaggt
    30001 attgctggca ttggccccgc cctttccgtt gaacgtgtta ccaccggagg cttcgacatc
    30061 ggcacgagcg ttaccaaaca gcccctgcaa atagcgcggc acagtatcaa agccaaaatt
    30121 agttttaccg tcacggctgg cattcgcaga ggagcttttg ctggcgctga cgttctcctg
    30181 caacacgatg gtcagcgtat cgccaatatt gcgtggtcga cgatcttcaa acagcggttg
    30241 atagccatag ttaatcggct gagcagactg gaaaatagaa ccgttggcga cgggcgtcgg
    30301 accgggaacc ggttgtgcac tggtcgcccc ctgcaccagc ggcgtggagg gtatccaggc
    30361 gcagccggtt agtgaaagca ccaacaagct ggaaatggca taagtatgcg cagcgttttt
    30421 ttgcatggct atcatcttca aaaatcagta aaccggtgaa cctgccaccg gttatgcctt
    30481 agagttgcgt cagtttttgc agcatctgat cggtggtgga caccgcttta ctgttgattt
    30541 cgtaagcgcg ttgcacctga atcatattga ccagttcttc cgccacgttg acgttagacg
    30601 tttcaacata cccttgatac agcagtcccg cgccgttcag gcccggcgtg ctttcgttcg
    30661 gtgcaccgga ggattgcgtt tcggtgtaga ggttttcgcc aatgctctcc agtccggtat
    30721 cattcataaa ggtggtgaga ttgagctgcc caacctgaac cggagctgcc tggccttgtt
    30781 gggttacgct gaccacgcca tcacgaccga tggtgatact taacgcattc gccggaatgg
    30841 tgatcgctgg ctgcacctga aaaccaccag ccgtcaccag ctgcccgttc tgatccacct
    30901 ggaaagagcc gtcacggata taggctgatg agccatctgg caacattacc tggaaaaagc
    30961 cctgcccttt aatcgcgaca tctttgctgt tattggtctg cgacagattg ccctggctgt
    31021 gtaagcgttc agtggcgacc gggcgtaccc ccgtgccgat ttgtaatccg gagggtaagg
    31081 tggtttgttc ggaagactgt gcccccggct ggcgaatggt ttgataaagc agatcttcaa
    31141 acaccgcgcg ctgacgctta aaaccgttag tactgacgtt tgccaggttg ttggcaatga
    31201 cgtccatatt ggtttgctgg gcgtcaaggc ccgttttggc gatccataat gaactgatca
    31261 tataatgtcc tgtagtggat tgctgacaag cagagcagaa gcacgcgaca gtgtgaattt
    31321 cccctcaccc taaccctctc cccaaagggg cgagggaata gtccgcgcgc ttttattgca
    31381 atgtcatcaa tcgttcaatt aactcatcga cagcagttgg ttggcacggc ctgcgttatc
    31441 atcgacgctg ctgatcacct tcatctgcat ttcaaaacgc cgcgcgctgg caatcatgtc
    31501 gctcattgcc gcaacggcat tgacgttact gccttccaga acccccgaca tcacacgcaa
    31561 ggttggatct gcctgcagta ccggccctcg cgtggcctgg gtttctgcgc ttaaacgaaa
    31621 aatgccgtcg tcaccgcgct gcacttcgct gcccgtggct ttcaccagtt tcagacgccc
    31681 tactggcgca actgtatttg ccggatcgcc cggattgagc gccgagattg tgccatcggc
    31741 agcaatcgtg atttccgccc cttccggcac agcaattggc cccgcctcgc ctatcaccgg
    31801 atgcccctga attgtcagtt gcccggtggg atcaacctga atgctgccat tacgcgtata
    31861 cccttcgctg ccgtcagcgg tctgcacggc cagccatcct gctgcaacgc aacgtccagc
    31921 gggcgagagg tgtaatccat cttgccgggc gtcatgtctg cacccggtgt tgacgccgtt
    31981 accagcgtgc gcgtgggcag tgaaagccct tccaccggca ctgcgcgcaa ggcgtttaac
    32041 tgcgcgcgaa aaccgggcgt agaggcattg gccagattac tggcggttac cgcctgttga
    32101 ttcagcgtct ggctggctgc tcccatcgca gtataaattg cgtgatccat tgagctatcc
    32161 cgtcagcgat tagcgtaagt taacccgcgt gttgaggatc tggtcctggg ttttgatggt
    32221 ctgggcgtta gacttatagt tacgctgggc aacgatcata ttgaccagtt ctttactgag
    32281 atcgacgttg gacgcttcca gcgcaccgtt ggtcagagtg ccaaagtttc ccgtcccggc
    32341 tgtccccaac agcgccacgc cagaagattg cgtcgcagac cagacgttgt cgccttcgga
    32401 tgccagacct tcgttgttgg caaagttcgc cagtacaatc tgccccagca gttgggtttg
    32461 ttcgttggaa ttgttgccga caaccgtacc gtcatcattg atttgataac tcaccagatc
    32521 gcccggtttg tagccattct gggtggttgc cacaatgttg ttagcgccgg ttgcgatatt
    32581 attcgccatc gcaccatcca ctaatgtgcc attagcatta aattccagtg ttgtcgctgt
    32641 cttcgcaatg ctgtttggat cactgctatc ctgggtgtag acctgccagt tattatcccc
    32701 ggtcttcaca aaatagacgc tcatgtcatg agcattaccc tgactgtcga aaacagtcac
    32761 cgaacctttt ttgttatagc tatccgcatt gctggcgctg aatggcgtaa cagtaggaag
    32821 cggatcactg gaattcaggt tgatctgcat cgacgccgtg gtggtagttt tcgctgccat
    32881 cagggtattc gggatcgaaa tattggtcgg attcgcccct tgctgaatag tcggcggcgt
    32941 accggttacc gggtaacccg tcagctgtaa accttgcgta ttcaacaggt tacggttttc
    33001 atccagctta aattgtccgt tacggctgta gaacaccgaa ccgttgctgt ctaccagacg
    33061 gaaaaaaccg ttctggctga tagcaacgtc cagacctcgc ccggtgttgg tggtcgtgcc
    33121 atcggtaaag tcctgagtga taccggcaac ttttaccccc agtcccactt tcgaaccggc
    33181 aaacatatcg gcaaaagagg ccgtgcctga tttaaagccg taggtggcgg agttggcgat
    33241 attgttgcca ataacatcga ggttggtggc ggcagcgttt aatccgctaa ccgcttgaga
    33301 aaaggccatg actgactcct gaaatgtgaa ggcttaaatt atctgccgta cttcgtcgag
    33361 ggtggtggtg ccgtaagtgc cgagatccag cgtattaccg ctgttgccgc ggatcacacc
    33421 ctgcaccaga gcaaactgca gcggctgggc aaccagttgt gtaccaccgt tactggcgct
    33481 aatcgctaca ttgtaagaac cgttcggcgc agtgctgcca tcagtcaacg aaccgtccca
    33541 ggtgaaactg tgaactccgg cggtcagttc accaatatca atggtgcgca caaccgcgcc
    33601 atttttatcg gtgatggtgg ccgtaacttt gtctgccgcc tgttgcagct caacaccaaa
    33661 cggcgtggtc gtggtcacag ccccttcttc actgccggtt cccgcaagaa cagttgtgcc
    33721 ggggatcatc acgccgtgac cgatcaggtt actggcctgt aacgactggc tgttatcaat
    33781 ctgtccggaa atagatccga gcgtggtatt gagtttttca atcccgctga ccgtgctgat
    33841 ttgtgccaat tgcgacgtca gctcgttgtt ttccattgga ttggtcgggt cctggttttt
    33901 cagctgcgcc accagcaaag tcagaaaact gctttgtaaa tctgcggcgt tgctgcccgt
    33961 gagcgaacta ctgctggtgg tactgacgcc ggtatttgtc ggatcggtgg tggttaccgc
    34021 aatggacata gctttctcct ttattgaccg agcgtaaggg ttttcagcat catgcttttc
    34081 accgtgttga gcacttcaac attggcctga tagctgcgtg acgccgacat ggtgttaacc
    34141 atctccccga cgacatcaac gttcggcatt tttatgtagc cctttgcatc tgccagcgga
    34201 ttacctggtt cataaaccag tttgtccggg gcctgacttt cttaacatcg gcaaccttta
    34261 cgccgcctgt cgcagcacct ggtgcagcgt taacctggaa taccacctgt tttgcccgat
    34321 atggctgtcc atcaggaccg gtcacgctat cagcatttgc cagattactg gccgccacgt
    34381 tcaggcgctg ggactgggca gttaacgccg acccggcgat atcaaaaata ttcagcagtg
    34441 ccatccgtta atttccgctc tgtaaaacgt tcatcatgcc tttgatttgc ccgctcaacg
    34501 cgctaaggct catctggtat tgcaggctgt tatcggcaaa ctgggtgcgt tcgcgatcca
    34561 tatcgacggt attaccgtca agcgaaggct ggtccggaat acggtattgc agttctgcgg
    34621 taggaggcgt cagcgcctgc gccgggatgt gctgcgttga ggtcatcgtc agtgcaacca
    34681 cactggttgc atcccgtcca cgttgcatga cttttttaag ttcactggca aaatcgatat
    34741 cgcgcgcctg ataaccaggg gtatcggcat tggcgatgtt tgctgccagc acttcctgac
    34801 gctgggcgcg cagattgagc gcctcttgtt gaaaacgtaa ggcggcgtcg agcttatcga
    34861 gcatatctcc tccgcaggta tcaaaattct gccatcagct taaatgcctt tacgcgcgcg
    34921 ttatcggcgg aataaacgca aaatgggtcg ctatttatgc cgttgatggt cattgcggac
    34981 aggtacaatt cacgttgtag aaatggctgg gggcgaaaat gctgacaata aaacgtagcg
    35041 tggcgatcat cgcgatactg ttcagtccgt taagtacggc gagcaatctc acatcgcaat
    35101 tgcacaactt ttttagcgcc caactcgcgg gggtaagtga tgaggttcgt gtttctattc
    35161 gtacagcgcc caatctacta ccgccatgcg agcagccatt gctttcgatg agcaataatt
    35221 cccgcctgtg gggcaatgtg aatgtgttgg cacgctgcgg taacgacaaa cgatatttac
    35281 aggttaatgt gcaggccaca ggaaattatg tggttgccgc gatgcctatt gtgcggggag
    35341 gaaagctgga agctggcaat gtcaaactga aacgtggacg cctggatacc ctgccaccgc
    35401 gtacggtgct ggatatcaat caacttgttg atgccgttag cctgcgcgat ctatcacccg
    35461 atcaacctat ccagttaacc cagtttcgcc aggcatggcg ggtaaaagcg ggacaacgcg
    35521 tcaatgtgat cgccagcggt gatgggttta gcgccaacgc agaaggtcag gcgctgaaca
    35581 atgcagccgt cgcacagaat gcgcgggtgc gcatggtatc ggaacaggta gtcagcggcg
    35641 ttgttgatgc agatgggaat attcttataa acctgtaagc tgttaaagat tacccgtccc
    35701 ttgccgataa ataagcaaca catgataaaa gcgccctcaa tgaggaataa accatgagta
    35761 ttgatcgcac ttcgcctctg aagcctgtaa gcaccgttca accgcgcgaa accaccgacg
    35821 cgccggtaac gaacagccgg gcggcaaaaa caaccgcctc caccagcacc agtgtgacgt
    35881 taagcgacgc gcaagcaaaa ctgatgcaac ccggcagcag tgatatcaat cttgaacgtg
    35941 tcgaagcgtt aaaactggcg attcgtaacg gtgaactaaa aatggacacc ggcaaaattg
    36001 ccgatgcgct gatcaacgaa gcgcagcaag acttgcagag taactgatcg tatgacacgt
    36061 cttgcagaga tcctcgacca gatgtccgct gtgcttaacg atctcaaaac ggtaatggat
    36121 caagagcagc aacatctctc tatggggcag atcaacggca gccagttgca atggattaca
    36181 gaacaaaaaa gctcactgct ggcgacgctg gattacctcg aacagttacg caagaaagaa
    36241 cccaatacag caaatagcgt tgatattagt caacgctggc aggaaattac tgtgaaaacg
    36301 cagcaactac gccaaatgaa tcaacataac ggctggttac tggaaggaca gattgagcgc
    36361 aatcaacagg cgctggaaat gttgaaaccg catcaggaac cgacgctata tggggcgaac
    36421 ggtcagacct caacacccca tcgcggcagt aaaaagattt cgatctgaat attatctccg
    36481 gcctgcactg caggccggaa tgcattgtta caccgtccgg cgggcaaatt ctttaacttt
    36541 gaagcccagt accgccagtg cagcgaagta cgcggcaatc cccgccagca cgaccgccat
    36601 taaacgcagt aagcgccagg gcatggtacc caatgaccac tccggcatga tatgtaacat
    36661 acctaaaagc acgccagaca tcaccagtac cgccaccacc agacgcaaca gaaacgccat
    36721 ccagccgggt tgcggagtaa agattttctg cttacgcaac tgccagtaaa gcagcgaagc
    36781 attcagacac gccgccagac caatagaaag tgacagcccg gcatgtttca acggaccaat
    36841 aaacgccagg ttcatcaatt gcgttaaaat cagcgtaacg atggcaattt tcactggcgt
    36901 tttaatgtcc tggcgggaat aaaagccagg agccaacact ttcactacaa tcaggccaat
    36961 caaacccacc gagtaggcaa ttaacgcccg ctgggtcatc agcgcatcaa acgcggtaaa
    37021 tttaccgtac tggaacagcg aaacggttaa cggacctgaa agaatgccca acgcaaccgc
    37081 actcggcagt gccaacagga aacaaagacg caacccccag tccatcaaac ggttgtattc
    37141 atcatgattg ccactggcaa aacttttcga cagcgacggc agcaaaatgg tgccaagcgc
    37201 cacgcccagc acaccggagg gaaactccat taagcggtcg gcgtaataca tccaagacac
    37261 cgaaccggaa gcaagaaacg aggcaaaaat ggtgttgatg attaaggaga tctggctcac
    37321 agagacgcca aggatcgccg gtcccatctg tttcaccacg cgcattgctc cagcatcgtg
    37381 gaagttaatg cgcggcagga ccagcatgcc gatcttcttc aggtgcggta gctgatacac
    37441 cagctgcaga atgccgccga ccgtaacagc ccacgccagc gccagcaccg gtgggttaaa
    37501 gtacggtgcg gcaaacagcg cgaaaccaat catgctgatg ttaagcagtg ttggcgcaaa
    37561 cgccggaatc gagaagcggt tccacgtatt cagaatcgct cccaccagcg acgccaggga
    37621 gatcagcaag atatagggaa aggtaatctt tagtagctgg ctggtcaggg caaatttgtc
    37681 agctgtgtca gcgaagcctg gcgcggtcac catgatcacc cacggtgcgg cgagcatgcc
    37741 agcgaccgtc acaaccgcca gcgcaagtgt cagcaggcca gaaacataag agacaaagac
    37801 ccgcgtggcg tcttcaccct gcttactttt atattccgcc agaatcggta caaatgcctg
    37861 ggaaaatgcc ccttcggcaa agatacggcg taacaagtta ggaagtttaa aagcgacgaa
    37921 aaaggcgtcg gttgccatcc ctgcgccaaa gattctggcg acaattgcgt ctcgtgcgaa
    37981 gccaagcaca cgcgaaaaca tggtcatcga gctgacggcg gccagcgatt ttaataaatt
    38041 catcggtgtt ctaatccgga cccacgggca aaacgcctgc aaggcaggcg ctgaaagagg
    38101 cgaatagtct acctagatta tggtgaattg ctaccgccag atgttacagg gtttattcac
    38161 tcatcgcatc gcgccagatc ttgtcaacga tacgttgcgc cagcactgcc tattcgccgg
    38221 cggtttgcgg aactgtctga ttttacacgc attcaatgaa gtgccgcgca cagccgacaa
    38281 atccacgttg ctcaagagtg ctctgccaac caggaatcgg tttattcact acaccctgcc
    38341 cgcgctcctc acgccattcg cgcatatccg taatgtcgat gagcgcaccg tcagtcacgg
    38401 cctgcacggt ttcacgctga cttccggcac ggcgatgcat acaagtggtg atctgcaaag
    38461 gaccggccga aaaatggtgc tcggcaaaca gcatttcgcc agcgtcgtta gtcagtagcg
    38521 taccgccatc cagagaggct ttgccgcccg acaaccacag cgcggtatcc accacatgca
    38581 gataatcatc cagcaacgtg aaataaagat cgtgtggccc gacgctattg ctacgatgtt
    38641 tatccattct tagcgaggct gcggtggcga gttgcgtttt taactcaccg tagagtggtg
    38701 cgaaacgacg gttaaaaccg accatcaacg tcagtttttt ccgcgccgcc agttccacca
    38761 gcctttcagc atcgcgcaga ttttctgcca gcggtttatc gacacagaca tgtacccccg
    38821 cattgagtaa cgtactgacc acgtcaaagt ggctggcggt gctggagtgc acaaaaaccg
    38881 catcacaact ggcggcaagg ctggataacg aatcggcata aggaatgcgc cagctgtcac
    38941 aaattggcag ggctttggcg cgcgtaggcg accaggctcc ttgtaacgtc cagtcagacg
    39001 ctgccgccag caccggtaac cacgcttttt gcgcaatgcc gcctaatccc actacgccga
    39061 tacgtaattt tttcacttta atctcccaga tgggccagca acgaatcaag acgttgtttc
    39121 agttctgcca cttcgatttc cagggcttcg acgcgggtct gtaaatcacc gtcaaccatg
    39181 ttcgacatag ccgttaccgc cggctgatct tcaacctcac cactgaaaag atgcatgtag
    39241 cggctttcgc gtttacccgg ttcgcgggcc agacgcacca caaaaggacc atcttcgcga
    39301 ttcgccagtt gttccagcgt cgactccact tccgccatat cgctgaattc atacattcgc
    39361 gcggcgcggc tgcgcagttc acccggcgtc tgggcaccac gcaataacaa cgtggtgatt
    39421 aacgccactt ctgcggcgct cagtttcaga tcgccaaatt ctgaattaca aaaacgttgc
    39481 tcatatttgg tgacccgatt accaaaaccg ctcactgtgc gtagataatg acgtttgacc
    39541 agattatcca gctgttcctg cacttcggat tcactcagat tcatgaccgg ttcacggttc
    39601 gttttctgat tacaggccgt gactacacca ttgactgaga gcgggtattg ctccggcgtg
    39661 gtcacctgct tttccagcaa gcaaccaatt acgcgagctt ccagggcagt aagttgatat
    39721 ttcatgagat tctccttagc ggccgggcgt ccagtctggg gtagttaatg ccgtcagtac
    39781 gtgatcgcgc cattgtccat caatcaacag atagtctttc gcatagcctt ctttttcaaa
    39841 acccagtcgc gccagtaaat caccgctgcg tttattgtgc ggcatataat tagccataat
    39901 gcgatgaata tgttgggtgc gctgcatata acgaatggct gcggtcaggg cttcaaacat
    39961 gagtcctttg ccctgccatt tttgcccaat cgaataaccg agatagcagg catgaaaaga
    40021 gccgcgaaca acattggaaa aattggcaac gccaataatc tctttttcgt ccgggtcgaa
    40081 taagccaaag tagaaagctg aaccttgttt atgaaattcg ttaatcatcc ctagcctggc
    40141 ctgccagcct gatggataac agtggctttc gtcgcgcact ggctcccagg gcttgaggaa
    40201 atggcgattc tctgcgtaat aatccgccag acgccaggca tcacgatcat gcaccagacg
    40261 cacgaccagt cggtctgtgg ttaagcgcac ttttggcacg ttactgcgat agccaaacat
    40321 tctataccta ctccttcccg taacgtcttt aatacgctgt aagtattact atacctgcgc
    40381 aaccagaccc tgtgaaaagt ccaaaatgcc attttctgca ttattcacat atttgataaa
    40441 aactctctat caaagcgacg ttttgataaa aaaatactgt ccccgaagtg atgggaaaag
    40501 tcttagcggc aatgcagaat agaaagtgct catcttttat ttcccgggag gggaaatgtc
    40561 ccgcgtatcg caggcgagga acctgggtaa atatttcctg ctcatcgata atatgctggt
    40621 cgtgctgggg ttctttgttg tcttcccgct gatctctatc cgcttcgttg atcaaatggg
    40681 ctgggccgcc gtcatggtcg gtattgctct cggtctacgc cagtttattc agcaaggtct
    40741 gggtattttc ggcggcgcaa ttgccgaccg ctttggtgcc aaaccgatga ttgttaccgg
    40801 tatgctgatg cgcgccgccg gattcgccac aatgggtatc gcccacgaac cgtggctatt
    40861 gtggttttca tgcctgctct cgggactcgg cggcacgttg tttgatccgc cgcgttcggc
    40921 gctggtggtg aaactaatcc gtccacaaca acgtggtcgt tttttctcgc tgttgatgat
    40981 gcaggacagt gccagtgcgg tcattggcgc attgttgggg agctggctgt tgcaatacga
    41041 ctttcgcctg gtctgcgcca caggggccgt tttgtttgtg ctatgtgcgg cgttcaatgc
    41101 gtggttgtta ccggcatgga aactctccac cgtacgcacg cccgttcgcg aaggcatgac
    41161 ccgcgtgatg cgtgacaagc gttttgtcac ctatgtcctg acgctggcgg gttactacat
    41221 gctggctgta caagtgatgc tgatgctgcc aattatggtc aacgacgtgg ctggcgcgcc
    41281 ctctgccgtt aaatggatgt atgccattga agcgtgtctg tcgttaacgt tgctctaccc
    41341 tatcgcccgc tggagtgaaa agcattttcg tctggaacac cggttgatgg ctgggctgtt
    41401 gataatgtca ttaagcatga tgccggtggg catggtcagc ggcctacaac aacttttcac
    41461 cctgatttgt ctgttttata tcgggtcgat cattgccgag cctgcgcgtg aaaccttaag
    41521 tgcttcgctg gcggacgcaa gagctcgcgg cagctatatg gggtttagcc gtctgggtct
    41581 ggcgattggc ggcgctattg gttatatcgg tggtggctgg ctgtttgacc tgggcaaatc
    41641 ggcgcaccag ccagagcttc cgtggatgat gctgggaatt attggcatct tcactttcct
    41701 tgcgctgggt tggcagttta gtcagaaacg cgccgcgcgt cgtttgctgg aacgcgacgc
    41761 ctgatttgat actcccccct gactcctcca tactgaaagt gaactgacag ccaaatggag
    41821 gagtcacgtg aagctataca tttacgatca ctgcccttac tgcctcaaag cccgcatgat
    41881 tttcggcctg aaaaatatcc ccgtcgaatt acatgttctg ctcaacgacg acgcagaaac
    41941 acccacccgg atggtcggtc aaaaacaggt tcccattctg caaaaagatg acagccgcta
    42001 tatgccagaa agcatggata tcgttcacta tgtcgataaa ctcgacggca aaccgttact
    42061 gaccggcaaa cgttcccctg ccattgaaga gtggctgcgc aaggtcaatg gctacgtcaa
    42121 caaactgctg ttgccgcgtt ttgccaaatc ggcatttgat gagttttcta ctcccgccgc
    42181 gcgcaaatat ttcgtcgaca agaaagaggc cagcgcgggt aattttgccg acctgctggc
    42241 ccactctgac ggtctgatta agaatatcag cgatgatttg cgtgcgctgg acaaactgat
    42301 cgtcaaaccg aacgccgtga atggcgaact ttcggaagat gatattcagc tatttccgct
    42361 actgcgtaat ctgacgctgg tagccggaat caactggcca agccgcgttg ctaattaccg
    42421 cgataatatg gcgaaacaga cacaaatcaa tttgttatca tcaatggcga tttaatcctg
    42481 acccgccgcg gcagcctgta ttcgcgggtt gccgccgttt tcagtttttt ctgctggcga
    42541 gtttatcgct ggtgacacat gctgcgttgt gtcaggatat tgacgcagat ttcatgtcga
    42601 ggactaccat gaacaagttt ttatttgccg ctgcattgat cgtcagtggc ctgctcgttg
    42661 gctgtaatca actcacccaa tacaccatca ccgaacaaga aattaaccag tcgcttgcga
    42721 aacataataa tttctcaaaa gatatcggtt tacccggcgt ggctgacgcc catattgttc
    42781 tgacaaactt gaccagccaa attggtcgcg aagagccgaa taaggttacc ctaaccggag
    42841 acgccaatct ggacatgaac tccctgttcg gtagtcagaa agcgaccatg aaactgaagc
    42901 tgaaagcgct gcccgtgttt gataaagaaa aaggtgcgat cttcctgaaa gagatggaag
    42961 tggtcgatgc gacggtacaa ccggaaaaaa tgcaaacggt gatgcaaacg ttgcttccct
    43021 atttgaacca ggcattacgc aattacttta accagcaacc tgcttacgtc ctgcgcgaag
    43081 atggcagtca gggcgaagca atggcgaaaa aactggcgaa aggcattgaa gtgaagccag
    43141 gcgaaattgt cattccgttt acggattaat gacgagggcg cattcgcgcc ctttattttt
    43201 cgtgcaaagg aaaacgtttc cgcttatcct ttgtgtccgg caaaaacatc ccttcagccg
    43261 gagcatagag attaatgact gcaccatccc aggtattaaa gatccgccgc ccagacgact
    43321 ggcaccttca cctccgcgat ggcgacatgt taaaaactgt cgtgccgtat accagcgaaa
    43381 tttatggacg ggctatcgta atgcccaatc tggctccgcc cgtgaccaca gttgaggctg
    43441 ccgtggcgta tcgccaacgt attcttgacg ccgtacctgc cgggcacgat ttcaccccat
    43501 tgatgacctg ttatttaaca gattcgctgg atcctaatga gctggagcgc ggatttaatg
    43561 aagccgtgtt caccgctgca aaactttacc cggcaaacgc aaccactaac tccagccacg
    43621 gcgtgacgtc aattgacgca atcatgccgg tacttgagcg catggaaaaa atcggtatgc
    43681 cgctactggt gcatggtgaa gtgacacatg cagatatcga catttttgat cgtgaagcgc
    43741 gctttataga aagcgtgatg gaacctctgc gccagcgcct gactgcgctg aaagtcgttt
    43801 ttgagcacat caccaccaaa gatgctgccg actatgtccg tgacggaaat gaacggctgg
    43861 ctgccaccat cactccgcag catctgatgt ttaaccgcaa ccatatgctg gttggtggcg
    43921 tgcgtccgca cctgtattgt ttacccatcc tcaaacgcaa tattcaccaa caggcattgc
    43981 gtgaactggt cgccagcggt tttaatcgag tattcctcgg tacggattct gcgccacatg
    44041 cacgtcatcg caaagagagc agttgcggct gcgcgggctg cttcaacgcc ccaaccgcgc
    44101 tgggcagtta cgctaccgtc tttgaagaga tgaatgctct gcagcacttt gaagcattct
    44161 gttctgtaaa cggcccgcag ttctatggct tgccggtcaa cgacacattc atcgaactgg
    44221 tacgtgaaga gcaacaggtt gctgaaagca tcgcactgac tgatgacacc ctggtgccat
    44281 tcctcgccgg ggaaacggta cgctggtccg ttaaacaata aaaatcgtag cttcctgttg
    44341 tcattaggtt attttacctg tataaataac cagtatattc aacagggggc tattatgcga
    44401 attgaagtca ccatagcgaa aacttctcca ttgccagctg gggctattga cgccctggct
    44461 ggcgaacttt cccgccgtat tcagtatgcg tttcctgata atgaaggcca cgtatcggta
    44521 cgttatgccg cagcgaataa tttatcggtt attggcgcaa caaaagaaga taaacagcgc
    44581 attagcgaaa ttctccagga aacgtgggaa agcgccgatg actggtttgt cagcgaataa
    44641 tatgcagtga ttt
//
LOCUS       DAZPAB010000011        44598 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZPAB010000011 DAZPAB010000000
VERSION     DAZPAB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.011"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            170..988
                     /gene="ybhA"
                     /locus_tag="ACHUPG_000550"
     CDS             170..988
                     /gene="ybhA"
                     /locus_tag="ACHUPG_000550"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFR0860294.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(989..2047)
                     /gene="modC"
                     /locus_tag="ACHUPG_000551"
     CDS             complement(989..2047)
                     /gene="modC"
                     /locus_tag="ACHUPG_000551"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR0860295.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2050..2739)
                     /gene="modB"
                     /locus_tag="ACHUPG_000552"
     CDS             complement(2050..2739)
                     /gene="modB"
                     /locus_tag="ACHUPG_000552"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR0860296.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2739..3512)
                     /gene="modA"
                     /locus_tag="ACHUPG_000553"
     CDS             complement(2739..3512)
                     /gene="modA"
                     /locus_tag="ACHUPG_000553"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0860297.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3679..3828)
                     /gene="acrZ"
                     /locus_tag="ACHUPG_000554"
     CDS             complement(3679..3828)
                     /gene="acrZ"
                     /locus_tag="ACHUPG_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR0860298.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3957..4745
                     /gene="modE"
                     /locus_tag="ACHUPG_000555"
     CDS             3957..4745
                     /gene="modE"
                     /locus_tag="ACHUPG_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR0860299.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4813..6285
                     /gene="modF"
                     /locus_tag="ACHUPG_000556"
     CDS             4813..6285
                     /gene="modF"
                     /locus_tag="ACHUPG_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR0860300.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6546..7562
                     /gene="galE"
                     /locus_tag="ACHUPG_000557"
     CDS             6546..7562
                     /gene="galE"
                     /locus_tag="ACHUPG_000557"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR0860301.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7572..8618
                     /gene="galT"
                     /locus_tag="ACHUPG_000558"
     CDS             7572..8618
                     /gene="galT"
                     /locus_tag="ACHUPG_000558"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR0860302.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8622..9770
                     /gene="galK"
                     /locus_tag="ACHUPG_000559"
     CDS             8622..9770
                     /gene="galK"
                     /locus_tag="ACHUPG_000559"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR0860303.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9764..10804
                     /gene="galM"
                     /locus_tag="ACHUPG_000560"
     CDS             9764..10804
                     /gene="galM"
                     /locus_tag="ACHUPG_000560"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR0860304.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11007..11759
                     /gene="gpmA"
                     /locus_tag="ACHUPG_000561"
     CDS             11007..11759
                     /gene="gpmA"
                     /locus_tag="ACHUPG_000561"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR0860305.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11926..12978)
                     /gene="aroG"
                     /locus_tag="ACHUPG_000562"
     CDS             complement(11926..12978)
                     /gene="aroG"
                     /locus_tag="ACHUPG_000562"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR0860306.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13294..13674
                     /locus_tag="ACHUPG_000563"
     CDS             13294..13674
                     /locus_tag="ACHUPG_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR0860307.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            13788..14729
                     /gene="zitB"
                     /locus_tag="ACHUPG_000564"
     CDS             13788..14729
                     /gene="zitB"
                     /locus_tag="ACHUPG_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR0860308.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(14726..15445)
                     /gene="pnuC"
                     /locus_tag="ACHUPG_000565"
     CDS             complement(14726..15445)
                     /gene="pnuC"
                     /locus_tag="ACHUPG_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR0860309.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15483..16526)
                     /gene="nadA"
                     /locus_tag="ACHUPG_000566"
     CDS             complement(15483..16526)
                     /gene="nadA"
                     /locus_tag="ACHUPG_000566"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR0860310.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(16775..16850)
                     /locus_tag="ACHUPG_000567"
     tRNA            complement(16775..16850)
                     /locus_tag="ACHUPG_000567"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16815..16817),aa:Lys,seq:ttt)
     gene            complement(16854..16929)
                     /locus_tag="ACHUPG_000568"
     tRNA            complement(16854..16929)
                     /locus_tag="ACHUPG_000568"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16894..16896),aa:Val,seq:tac)
     gene            complement(16981..17056)
                     /locus_tag="ACHUPG_000569"
     tRNA            complement(16981..17056)
                     /locus_tag="ACHUPG_000569"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17021..17023),aa:Lys,seq:ttt)
     gene            complement(17059..17134)
                     /locus_tag="ACHUPG_000570"
     tRNA            complement(17059..17134)
                     /locus_tag="ACHUPG_000570"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17099..17101),aa:Val,seq:tac)
     gene            complement(17270..17345)
                     /locus_tag="ACHUPG_000571"
     tRNA            complement(17270..17345)
                     /locus_tag="ACHUPG_000571"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17310..17312),aa:Lys,seq:ttt)
     gene            complement(17510..18301)
                     /gene="cpoB"
                     /locus_tag="ACHUPG_000572"
     CDS             complement(17510..18301)
                     /gene="cpoB"
                     /locus_tag="ACHUPG_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR0860311.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18311..18832)
                     /gene="pal"
                     /locus_tag="ACHUPG_000573"
     CDS             complement(18311..18832)
                     /gene="pal"
                     /locus_tag="ACHUPG_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR0860312.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(18867..20159)
                     /gene="tolB"
                     /locus_tag="ACHUPG_000574"
     CDS             complement(18867..20159)
                     /gene="tolB"
                     /locus_tag="ACHUPG_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR0860313.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20292..21533)
                     /gene="tolA"
                     /locus_tag="ACHUPG_000575"
     CDS             complement(20292..21533)
                     /gene="tolA"
                     /locus_tag="ACHUPG_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR0860314.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(21598..22026)
                     /gene="tolR"
                     /locus_tag="ACHUPG_000576"
     CDS             complement(21598..22026)
                     /gene="tolR"
                     /locus_tag="ACHUPG_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR0860315.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22030..22722)
                     /gene="tolQ"
                     /locus_tag="ACHUPG_000577"
     CDS             complement(22030..22722)
                     /gene="tolQ"
                     /locus_tag="ACHUPG_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR0860316.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(22719..23123)
                     /gene="ybgC"
                     /locus_tag="ACHUPG_000578"
     CDS             complement(22719..23123)
                     /gene="ybgC"
                     /locus_tag="ACHUPG_000578"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR0860317.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23273..23566)
                     /gene="ybgE"
                     /locus_tag="ACHUPG_000579"
     CDS             complement(23273..23566)
                     /gene="ybgE"
                     /locus_tag="ACHUPG_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR0860318.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(23566..23679)
                     /gene="cydX"
                     /locus_tag="ACHUPG_000580"
     CDS             complement(23566..23679)
                     /gene="cydX"
                     /locus_tag="ACHUPG_000580"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR0860319.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(23694..24833)
                     /gene="cydB"
                     /locus_tag="ACHUPG_000581"
     CDS             complement(23694..24833)
                     /gene="cydB"
                     /locus_tag="ACHUPG_000581"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR0860320.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(24849..26417)
                     /gene="cydA"
                     /locus_tag="ACHUPG_000582"
     CDS             complement(24849..26417)
                     /gene="cydA"
                     /locus_tag="ACHUPG_000582"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR0860321.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27266..29901)
                     /gene="mngB"
                     /locus_tag="ACHUPG_000583"
                     /pseudo
     CDS             complement(27266..29901)
                     /gene="mngB"
                     /locus_tag="ACHUPG_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
     gene            complement(29919..31835)
                     /gene="mngA"
                     /locus_tag="ACHUPG_000584"
     CDS             complement(29919..31835)
                     /gene="mngA"
                     /locus_tag="ACHUPG_000584"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR0860322.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32005..32727
                     /gene="mngR"
                     /locus_tag="ACHUPG_000585"
     CDS             32005..32727
                     /gene="mngR"
                     /locus_tag="ACHUPG_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR0860323.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(32830..33699)
                     /gene="sucD"
                     /locus_tag="ACHUPG_000586"
     CDS             complement(32830..33699)
                     /gene="sucD"
                     /locus_tag="ACHUPG_000586"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR0860324.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(33699..34865)
                     /gene="sucC"
                     /locus_tag="ACHUPG_000587"
     CDS             complement(33699..34865)
                     /gene="sucC"
                     /locus_tag="ACHUPG_000587"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR0860325.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(34959..36176)
                     /gene="odhB"
                     /locus_tag="ACHUPG_000588"
     CDS             complement(34959..36176)
                     /gene="odhB"
                     /locus_tag="ACHUPG_000588"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR0860326.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36191..38992)
                     /gene="sucA"
                     /locus_tag="ACHUPG_000589"
     CDS             complement(36191..38992)
                     /gene="sucA"
                     /locus_tag="ACHUPG_000589"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR0860327.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39293..40009)
                     /gene="sdhB"
                     /locus_tag="ACHUPG_000590"
     CDS             complement(39293..40009)
                     /gene="sdhB"
                     /locus_tag="ACHUPG_000590"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR0860328.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40025..41791)
                     /gene="sdhA"
                     /locus_tag="ACHUPG_000591"
     CDS             complement(40025..41791)
                     /gene="sdhA"
                     /locus_tag="ACHUPG_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR0860329.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(41791..42138)
                     /gene="sdhD"
                     /locus_tag="ACHUPG_000592"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(41791..42138)
                     /gene="sdhD"
                     /locus_tag="ACHUPG_000592"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR0860330.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42132..42536)
                     /gene="sdhC"
                     /locus_tag="ACHUPG_000593"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42132..42536)
                     /gene="sdhC"
                     /locus_tag="ACHUPG_000593"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR0860331.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            42917..43024
                     /locus_tag="ACHUPG_000594"
     CDS             42917..43024
                     /locus_tag="ACHUPG_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0860332.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43230..44513
                     /gene="gltA"
                     /locus_tag="ACHUPG_000595"
     CDS             43230..44513
                     /gene="gltA"
                     /locus_tag="ACHUPG_000595"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR0860333.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT        10929 a        11940 c        10980 g        10749 t
ORIGIN      
        1 gagcatttgc ttcatgaatg ctcctttgca tttagcaact gactgtaaca gctaaaatta
       61 gtcgcttttg gcggtgaatg ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg
      121 tgtaccattc gcgcagacga aaattcttaa ccttaatctg gaatacgcca tgaccacacg
      181 cgtgattgct ctcgacttag acggcacctt attgaccccg aaaaagacct tgcttccatc
      241 atcgatagaa gccctggccc gcgctcgcga agcaggctat caattaatca tcgtcacagg
      301 tcgccatcac gtcgctattc atccttttta tcaggcgctg gcgctggata cacctgctat
      361 ttgctgtaat ggcacctatt tgtatgatta tcatgcaaaa accgtgctgg aagcggaccc
      421 aatgcccgtt aataaagccc tgcaactcat tgagatgctg aatgaacacc acattcacgg
      481 tctgatgtat gtcgatgatg caatggtcta tgagcacccg accgggcatg tcattcgcac
      541 gtctaactgg gcgcaaaccc tgccgccgga acagcgtccg actttcacac aagtcgcttc
      601 tctggctgaa acgacgcaac aagttaacgc cgtatggaag ttcgccctga cgcacgatga
      661 cctgccgcaa ttgcagcatt ttggtaagca tgtcgaacat gaactgggac tggagtgtga
      721 atggtcctgg cacgatcagg ttgatattgc acacggcggc aacagcaaag gtaaacgttt
      781 gacgaaatgg gttgaggcgc aaggttggtc gatggaaaac gtcgtggcat tcggcgataa
      841 ctttaatgat atcagtatgc tggaagccgc tggtacaggc gtggcgatgg gcaacgccga
      901 tgacgcggta aaagcgcgcg ccaacattgt gattggtgat aacaccaccg acagcattgc
      961 ccagttcatt tatagccacc tgatttaatc aggcggttat cgacacactt ttaatttgcg
     1021 cgtacagcca caggccaggt ttgatcgcca gttcatccct ggcccacggg ctgatacgcg
     1081 cccacagcgt tttaccgccg acttccagct ccacttccac ctggccgttg tcgtcataac
     1141 tattaacaac ttttgcccgc aatacgttac gaatgctggt ttgctgcggc ggttgtaaaa
     1201 ccagagaaac atcggaagcc tgaatgcgga tacgtagcgc agcttgcagc ggttcgccca
     1261 gcttattgac ccacaaatgc tgatcgccca gcgccagcgc ggtcatcgcg taatgcggat
     1321 gatgctcaag caccgtcact ttcagaatgc tactttgttg ctctttcggc agccacggat
     1381 tcatcacgct actgccccac acttcctcca gcgcgccaaa ggctttcacc tgaccgtttt
     1441 ccagtaccat cactctgtct gccagatgga ggatctcatc cagcgaatgg ctgacataca
     1501 acatcggaat gttgatttcc cgtgtcagcc gttgcagata aggcaacagt tcgcgtttac
     1561 gcggaatatc cagtgacgcc agcggttcat ccagcaacag caattccggt gctgtcagca
     1621 aagcccgacc aatcgccacg cgctgttttt cgcctccgga caggctgcct ggtaaacggt
     1681 caagcaacgg ttcaatgcct aaaagcgcca ccagcttatc gaactgatcg accatacttt
     1741 tcgacatgcc gtagcgcaga ttgccacgca ctttgtaatg cgggaacaac cgcgcatcct
     1801 gaaaaacata gccaacacga cgcttttccg gcgtcaggca gatacctttt tcggcatcat
     1861 ttagtacccg cccattgagg acaatccgcc ctttttgcgg gcgcgtcagt ccactgatgg
     1921 cgttaatcag cgaagttttt ccggcaccgg agacgccaaa gatagcagtg atgccattgg
     1981 cgggcagcgt ttcattaatg gtcaggcaat ggttgcccaa cgtctgggaa aaattcagct
     2041 ccagcatgat tagcgccccg cccgttcacg gctgattctg gccagccatt ctgaaatcaa
     2101 cagggagatc atcgccagcg caatagaaat aatgcacagt ctcgccgcgc cactttcccc
     2161 gccgggggtc tggatcaggg tatacatggc agaaggaatg gttcgcgttt cgctaggaat
     2221 gttcgacaca aaggtgatgg ttgcaccaaa ctcaccgaga gaacgagcaa aagccagtac
     2281 cgtaccaaca ataattcccg gtaaggtcag cggtaacgtg atagtaaaga aaacgcgcca
     2341 gcgcccggca cctagtgttc ttgcggcctg ttccagtttg acatcaaccc cttccagcgc
     2401 cagacgaatt gcccgcacca tcagcggaaa cgacatgacg gcggcagcga gaaccgcgcc
     2461 gcgccagcta aaggcgaagg taataccaaa ccagtcatac agacgttcac cgataaatcc
     2521 gcgccgtccc atcgaaacta ataataagta accgacgacc acgggcggta acaccagcgg
     2581 tagatgcagt acgctgtcga gcagagcttt gcccggaaac gtgcaacgca ccagtaacca
     2641 ggcaaaaaag atcccaaacg gcaggctaaa cagcacagcc agggaagaaa ctttcaggct
     2701 taataaaact gcctgccatt ctggatcggt cagtatcatt acttggttgt aaatccgtaa
     2761 cgtttaaaga tttccgctgc ctgcggtccc ttcagataat cataaaatgc tttcactgtc
     2821 gcattgttat gcccttccac aacagcaacc ggatattcca cttttttatg tgaatcttcc
     2881 gggaaggtgg caaccacttt tacccctttg ctggcaactg cgtcagaacc gtagacaatg
     2941 cccagaggcg cttcgttacg ttcgaccagc gccagcgccc cacgaacatc ttccgctggg
     3001 gccagtttcg gagagagcgt atcccatgcg cccagttttt gcagtgcttc ttttgcataa
     3061 atgccagcgg gaacatgttc cggatcgcca accgccaggc gaccgccatt cagcagcgaa
     3121 gtccagttag ttttgctgtc gatggtgaaa tctttctgct cgctggcttt cggtgctaca
     3181 acgaccaggc tattgccgag cagtgtctga cgcgtagcag tatcgatcgc ttttttatca
     3241 accgcataat ccatccattt ctgatcggca gaaataaaca gatccgcagg ggcacccgct
     3301 tcaatctgac gggcgagagt agatgacgaa gcgaaagaag aaaccacatc cacgcctttc
     3361 tcttttttat actgcgtagc aatgtcctgc attgcgttag tcagtgatgc ggcggcgaac
     3421 accgtgattt tcccttcatc tgccagtgca ttgccagcaa ctgcgaaaga gagtgccgcc
     3481 ccggcaaaca agttcaacca tttacgagcc attggtaacc ccttaatgta acgttatgta
     3541 ggcgacaata taacgactaa ctcggaattt tcccagcagt tattgctaac ctttgtgagg
     3601 tagataagaa aaaatatcgg caggaaaagc aggaagttga gaaaaagaaa atgcccgact
     3661 aagcgggcac tcagggaatc aatgattttg tccgggctgg tcttttttac caacaccaga
     3721 aaagatgttg aatacttcac caagaccgta aatcagaccc aggatgatgg ccatcacgac
     3781 aggtaccatg attacggcga ataccagact ttttaataac tctaacatgg tcaactccag
     3841 atatagtcat gagactattc taaccgctaa gcacagaaaa gcactcccct tttgtgcggt
     3901 cagctttgcg tggcgttcgt tttccgtcac aataagactt ttgccaggac attgttatgc
     3961 aggccgaaat ccttctcacc cttaagctcc aacaaaaatt attcgccgac ccgcgccgca
     4021 tttcgctact aaaacacatt gcgctttccg gttccattag ccagggagcg aaagatgccg
     4081 gtatcagcta taaaagcgcc tgggatgcca ttaacgagat gaatcagtta agtgagcata
     4141 ttctggtcga gcgcgcaaca ggcggtaaag gtggcggagg cgcagtactg acccgctatg
     4201 gtcagcgact gattcagctc tatgacttac tggcgcaaat ccagcaaaaa gcctttgatg
     4261 tgttaagtga cgatgacgcc ctgccgctga acagcctgct ggccgcgatc tcacgttttt
     4321 cactgcaaac cagcgcccgt aaccagtggt tcggtaccat caccgcccgc gatcatgatg
     4381 acgttcaaca gcatgttgat gtcttactgg ctgacggaaa aacacgcctg aaagtcgcaa
     4441 ttaccgcaca aagcggcgca cgtctggggc tggatgaagg caaagaagtg ttgatattgc
     4501 taaaagcgcc gtgggtaggc attactcagg acgaagcggt cgcacaaaac gccgacaacc
     4561 aattaccggg tattattagt catattgagc gcggcgcaga gcagtgcgaa gtattaatgg
     4621 cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt aaatgaagcg acttctcttc
     4681 agcaagggca gaatgtcacg gcctacttta atgccgacag cgtgattatc gccacgctgt
     4741 gctaagcgtg ttgacaattt gttatgaaac acgtatccct gtcagtaatc gctgcacaaa
     4801 atgggatata aaatgtcatc gttgcaaatt ttgcaaggca cgtttcgtct tagcgacaca
     4861 aaaacgctgc aattgcctca gctaacgtta aacgcgggtg atagttgggc gtttgtcggt
     4921 tcgaatggaa gtggaaagtc ggcgctggcc cgcgcgctgg cgggggaact tccgcttttg
     4981 aaaggtgaac ggcaaagcca gttttcccac atcactcgtc tctccttcga gcaattgcaa
     5041 aagctcgtca gcgacgaatg gcaacggaat aacaccgata tgctcggccc tagcgaagat
     5101 tacaccggac gcactacggc tgagattatt caggatgaag taaaggatgc accgcgttgc
     5161 atgcaactgg cgcagcagtt cggtattacc gccctcctcg accgacgctt taaatacctt
     5221 tccactggcg agacgcgaaa aaccctgctg tgtcaggcgc tgatgtcgga gcctgacttg
     5281 ttgattcttg atgagccgtt cgatggcctg gatgttgcct cacgtcagca gctggctgag
     5341 cgactcgcct cgttacatca gtccggtatt actttggtac tggtgctcaa tcgcttcgat
     5401 aagatcccgg aatttgtcca gtttgctggc gtgctggcgg attgtacgtt agcggaaact
     5461 ggcgctaaag aggaactgct ccagcaagca ctcgtcgcgc aacttgcgca tagtgaacag
     5521 cttgaaggtg tgcaactgcc ggagccggat gaaccttcag cacgtcacgc cttacccgcc
     5581 aacgaatcgc gcattgtgct gaacaatggc gtggtttctt ataatgatcg ccccattctt
     5641 aataacctta gctggcaggt gaatccaggc gaacactggc aaattgtcgg gccaaatggc
     5701 gcgggaaaat cgacgttatt aagcctgatt actggcgatc atccgcaagg ttacagcaac
     5761 gatttgacgc ttttcggccg acgtcgcggc agcagcgaaa ccatctggga catcaaaaag
     5821 catatcggtt acgtcagcag tagtttgcat ctggattacc gggtcagcac taccgtgcgt
     5881 aatgtgattc tttctggcta ttttgattcg attggcattt atcaggtcgt ttcggatcgc
     5941 cagcaaaaac tggtgcagca gtggctggat attctcggca ttgataaaca cacggctgac
     6001 gctccgttcc atagtctttc ctggggacag cagcgtttgg cgctgatcgt ccgcgcactg
     6061 gtgaaacatc cgacgttgct tattctcgat gaaccactac aggggcttga tccgctcaat
     6121 cgccaactta tccgccgttt tgttgatgtg ctgattagcg aaggtgaaac gcaattgttg
     6181 tttgtttcgc accacgctga agatgcgcct gcctgtatta cccatcgcct tgagttcgtg
     6241 ccggacggtg gactctatcg ctatgtgctg acaaaaatat attgagtcgg tagtgctgac
     6301 cttgccggag gcggcctaag cgcactctcc ggccaacggt tcgacgcatg caggcatgaa
     6361 accgcttctt ttttcagata aaaagcgcaa tcattcataa accctctgtt ttataatcac
     6421 ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg attccactaa tttattccat
     6481 gtcacacttt tcgcatcttt gttatgctat ggttatttca taccataagc ctaatggagc
     6541 gaattatgag agttctggtt accggtggta gcggttacat tggaagtcat acctgcgtgc
     6601 aattactgca aaacggtcat gatgtcatca ttcttgataa cctctgtaac agtaagcgca
     6661 gcgtactgcc tgttatcgag cgtttaggcg gcaaacatcc aacgtttgtt gaaggcgata
     6721 ttcgtaacga agcgttgatg accgagatcc tgcacgatca cgctatcgac accgtgatcc
     6781 acttcgccgg gctgaaagcc gtcgccgaat cggtacaaaa accgctggaa tattacgaca
     6841 acaatgtcaa cggcactctg cgcctgatta gcgccatgcg cgccgctaac gtcaaaaact
     6901 ttatttttag ctcctccgcc accgtttatg gcgatcagcc ccaaattcca tacgttgaaa
     6961 gcttcccgac cggcacaccg caaagccctt atggcaaaag caaattgatg gtggaacaga
     7021 tcctcaccga cctgcaaaaa gcccagccgg actggagcat tgccctgctg cgctacttca
     7081 acccggttgg cgcgcatccg tcgggcgata tgggcgaaga tccgcaaggc attccgaata
     7141 acctgatgcc atacatcgcc caagttgctg taggccgtcg cgactcgctg gcgatttttg
     7201 gtaacgatta tccgaccgaa gatggtactg gcgtacgcga ttacatccac gtaatggatc
     7261 tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa caagccaggc gtacacatct
     7321 acaacctcgg tgctggcgta ggcagcagcg tgctggacgt ggttaatgcc ttcagcaaag
     7381 cctgcggcaa accggttaac tatcattttg caccgcgtcg cgaaggcgac cttccggcct
     7441 actgggcgga cgccagcaaa gccgaccgtg aactgaactg gcgcgtaacg cgcacactcg
     7501 atgaaatggc gcaggacacc tggcactggc agtcacgcca tccacaggga tatcccgatt
     7561 aaggaacgac catgacgcaa tttaatcccg ttgatcatcc acatcgccgc tacaacccgc
     7621 tcaccgggca atggattctg gtttcaccgc accgcgctaa gcgcccctgg cagggggcgc
     7681 aggaaacgcc agccaaacag gtgttacctg cgcacgatcc agattgcttc ctctgcgcag
     7741 gtaatgtgcg ggtgacaggc gataaaaacc ccgattacac cgggacttac gttttcacta
     7801 atgactttgc ggccttgatg tctgacacgc cagatgcgcc agaaagcaac gatcctttga
     7861 tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg cttttcaccg gatcacagta
     7921 aaacgttgcc agaactgagc gttgcggcat tgacggaaat cgtcaaaacc tggcaggagc
     7981 aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt ctttgaaaac aaaggtgcgg
     8041 cgatgggctg ctctaacccg catccgcacg gacagatttg ggcaaatagc ttcctgccta
     8101 acgaagctga gtgcgaagac cgcctgcaaa aagaatattt tgccgaacag aaatcaccaa
     8161 tgctggtgga ttatgttcag cgcgagctgg cagacggtag ccgtaccgtt gtcgaaaccg
     8221 aacactggtt agccgttgtg ccttactggg ctgcctggcc gttcgaaacg ctactgctgc
     8281 ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc ccagcgcagc gatttggcgc
     8341 tggcgttgaa aaagctgacc agtcgttatg acaacctctt ccagtgctcc ttcccctact
     8401 ctatgggctg gcacggcgca ccatttaatg gcgaagagaa tcaacactgg cagctgcacg
     8461 cgcactttta tccgcctctg ctgcgctccg ccaccgtacg taaatttatg gttggttatg
     8521 aaatgctggc agagacccag cgagacctga ccgcagaaca ggcagcagag cgtttgcgcg
     8581 cagtcagcga tatccatttt cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa
     8641 cacaatctct gtttgccaac gcatttggct accctgccac tcacaccatt caggcgcctg
     8701 gccgcgtgaa tttgattggt gaacacaccg actacaacga cggtttcgtt ctgccctgcg
     8761 cgattgatta tcaaaccgtg atcagctgtg caccacgcga tgaccgtaaa gttcgcgtga
     8821 tggcagccga ttatgaaaat cagctcgacg agttttccct caatgcgccc attgtcgcgc
     8881 atgaaaacta tcaatgggcg aactacgttc gtggcgtggt gaaacatctg caactgcgta
     8941 acaacagctt cggcggtgtg gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt
     9001 taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt attgcagcag ctttatcatc
     9061 tgccgctgga cggcgcacaa atcgcgctta acggtcagga agcagaaaac cagtttgttg
     9121 gctgtaactg cgggatcatg gatcagctaa tttccgcact cggcaagaaa gatcatgcct
     9181 tgctgattga ctgccgctca ctggggacta aagcagtttc catgccgaaa ggtgtggctg
     9241 tcgtcatcat caacagtaac ttcaaacgta ccctggttgg cagcgaatac aacacccgtc
     9301 gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc agctctgcgc gatgtcacca
     9361 ttgaagagtt caatgctgtt gcacatgagc tggacccaat cgtggcgaaa cgcgtgcgtc
     9421 atatcctgac tgaaaacgcc cgcaccgttg aagctgccag cgcgctggag caaggcgacc
     9481 tgaaacgtat gggcgagttg atggccgagt ctcatgcctc tatgcgcgat gatttcgaaa
     9541 tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa agctgtgatt ggcgacaaag
     9601 gtggcgtacg catgaccggc ggcggatttg gcggctgtat cgtcgcgctg atcccggaag
     9661 agctggtgcc tgccgtacag caagctgtcg ctgaacaata tgaagcaaaa acaggtatta
     9721 aagagacttt ttacgtttgt aaaccatcac aaggagcagg acagtgctga acgaaactcc
     9781 cgcactggca cccgatggtc agccgtaccg actgttaact ttgcgtaaca acgcagggat
     9841 ggtagtcacg ctgatggact ggggtgcgac tttactttcc gcccgtattc cgctttccga
     9901 tggcagcgtc cgcgaggcgc tgctcggctg tgccagtccg gaatgctatc aggatcaggc
     9961 tgcgtttctg ggggcctcta ttggtcgtta tgccaaccgc atcgccaata gtcgctatac
    10021 ctttgacggt gaaaccgtga cgctttcgcc aagccagggc gttaaccagc tgcacggcgg
    10081 cccggaaggg ttcgacaaac gtcgctggca gattgtgaac cagaacgatc gtcaggtgct
    10141 gtttgccctg agttcagatg atggcgatca gggcttcccg ggtaatctcg gcgcgacggt
    10201 gcaatatcgt ctgaccgacg ataaccgtat ctccattact tatcgcgcca cagttgataa
    10261 accttgcccg gtgaatatga ctaatcacgt ctatttcaat cttgacggcg agcagtctga
    10321 cgtgcgcaat cacaagttgc agattctggc ggacgaatat cttccggttg atgaaggcgg
    10381 cattccgcac gacggcttga aatctgtcgc cggaacgtct tttgatttcc gcagcgccaa
    10441 aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc aaagtgaaag gttacgatca
    10501 cgcattcttg ttacaggcca aaggcgatgg caagaaagtg gcggcgcatg tctggtcagc
    10561 agatgaaaaa ttgcagctga aggtctacac caccgctccg gctcttcaat tctactccgg
    10621 caacttcctc ggcggcacac cgtcgcgggg aaccgaacct tacgccgact ggcaagggct
    10681 ggcgttggaa agcgagtttc tgccggacag cccgaaccac cctgaatggc cgcaaccgga
    10741 ctgcttcctg cgtcctggcg aagagtattc cagcctgacg gaatatcagt ttattgctga
    10801 gtaatgttaa gccctcgtca tgagggcttt atcttcatat tgttcaaatc accagcaaac
    10861 accgacatat ttgcaactca atattcacaa caaccttaca ctgcgccact attttcgcta
    10921 tggttatgcg taagcattgc tgttgcttcg tcacggcaat ataatgagaa ttattatcat
    10981 taaaagatga tttgaggagt aagcatatgg ctgtaactaa gctggttctg gttcgtcatg
    11041 gcgaaagtca gtggaacaaa gaaaaccgtt tcaccggttg gtacgacgtg gatctgtctg
    11101 agaaaggcgt aagcgaagca aaagcagcag gtaagctgct gaaagaggaa ggttacagct
    11161 ttgactttgc ttacacttct gtgctgaaac gcgctatcca taccctgtgg aatgtgctgg
    11221 acgaactgga tcaggcatgg ctgcccgttg agaaatcctg gaaactgaac gaacgtcact
    11281 acggtgcgtt gcagggtctg aacaaagcgg aaactgctga aaagtatggc gacgagcagg
    11341 tgaaacagtg gcgtcgtggt tttgcagtga ctccgccgga actgactaaa gatgatgagc
    11401 gttatccggg tcacgatccg cgttacgcga aactgagcga gaaagaattg ccgctgacgg
    11461 aaagcctggc gctgaccatt gaccgcgtga tcccttactg gaatgaaacc attctgccgc
    11521 gtatgaagag cggtgagcgc gtaatcatcg ccgcacacgg taactccctg cgtgcgctgg
    11581 tgaaatatct tgataacatg agcgaagaag agattcttga gctgaatatc ccgactggcg
    11641 tgccgctggt gtatgagttc gacgagaatt tcaaaccgct gaaacgctat tatctgggta
    11701 atgctgacga gatcgcggcg aaagcagcag ccgttgcgaa ccagggtaaa gcgaagtaaa
    11761 cgtcattcgt ttaaaaaatg agaaagccga ctgcaagtga gtcggctttt ttgtttgtcg
    11821 ttgctaacaa tggagcacat tgcctgatgc gacgctgcgc gtcttatcag gcctgtggtg
    11881 attcatcgga tacgccactc tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc
    11941 ttttactgca ttcaccagtt gacgtaacag agcatcggta tcttcccagc caatgcaggc
    12001 atcggtgatg ctcttaccgt aggccagcgg ctccccgcta tcgaggctct gattgccttc
    12061 caccagatgg ctttccacca tcacgccaat aatggccttt tcgccaccgg caatctgctg
    12121 gcaaacgtca gcacaaacat ccatctgctt tttgaattgt ttggacgaat tagcatggct
    12181 gaaatcgatc atcacctgag ctggcagacc tgctttgttc agcccttctt tcacttcagc
    12241 aacgtgcttc gcgctgtagt taggctcttt accgccgcgc agaatgatat ggcaatcgcc
    12301 gttaccactg gtattcacaa tcgccgaatg cccccattta gttacggaca ggaagcagtg
    12361 cggcgcaccg gcggcattaa tggcatcgat agccacttta atcgtaccgt ctgtgccatt
    12421 tttaaaacct accggacaag aaagaccaga cgccagttcg cggtgcacct gcgattccgt
    12481 ggtacgtgca ccaattgcgc cccagctcat caggtcagcg agatactgag gagtgatcat
    12541 atcgagaaac tcacccgccg ctggcagacc gctgtcgtta atatcaagca gcaatttacg
    12601 ggctatacgc agaccgtcgt tgatctggaa gctgttatcc atatgcggat cgttaatcaa
    12661 ccctttccag cccaccgtgg tacgcggctt ttcaaaatag acgcgcatta cgatttccag
    12721 ctcatctttc agctcttcac gcagcgccag caagcgagtg gcatactctt tagccgcgac
    12781 aggatcatga attgagcacg ggccaatcac caccaacagg cgatcatcat tacctttcag
    12841 gatcttatgg atcgcttttc gggcatgggc aaccgtattc gcggcatttt cagttgcggg
    12901 gaatttttcc agcaatgcga caggaggaag taactctttg atttctttga tgcgtaaatc
    12961 gtcgttctga taattcatgt ctgttccagt gttgccatac ttatcttagt gaatgcaata
    13021 cttccaatct atatcttccg tcagaatgtg taaacggggt tttacactat gaacggatta
    13081 atcctggaat tagcaagaaa aacgccagat tgtcgcgaaa aacgagatct ctcctacaat
    13141 ttctaactgt aactcctttg tttgttaatt atttcaagat tctctgctgc gtttcataaa
    13201 ctggctgaaa tcttaaacca atgccttata ttcacctgca aatgcactgt tggaagaggt
    13261 tatccgacat aacgaccata acaggagcat cctatgaaaa tgacaaaact ggccacatta
    13321 tttctgactg ccactctaag ccttgccagc ggtgccgcac tggctgccga tagcggagcg
    13381 caaactaata acggccaggc aaacgccgca gctgatgcgg gccaggtagc ccctgacgcc
    13441 cgtgaaaatg tcgcgccaaa taacgtcgac aataacgggg taaataccgg ttctggcggc
    13501 acaatgctgc attcggatgg ttcttcaatg aacaatgacg gaatgaccaa agatgaagag
    13561 cacaagaaca ccatgtgcaa agatggtcga tgtccggata tcaataaaaa ggtacaaacc
    13621 ggtgatggca tcaataatga tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc
    13681 aaaatagcca ataatgggag agcggagtct ctccctttta atttttcact tctaaaagca
    13741 gtatgatgtc ataaagttaa gtgagaataa ttaagggatg actcgtcatg gcgcactcac
    13801 actcacacac gtcctcacac ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt
    13861 tcggcgtgac tgccgggttt atgctggtag aagtcgttgg tggttttctt tctggttctc
    13921 tggcattgct ggccgatgcg ggtcatatgt tgaccgatac tgccgccctg cttttcgccc
    13981 tactcgccgt gcaattttca cgtcgtcctc ccaccattcg ccacactttc ggttggctaa
    14041 gactgaccac gctcgcggcg tttgtgaatg ccatcgccct cgtggtaatt accattttga
    14101 ttgtctggga ggcgatagaa cgcttccgca cgccgcgtcc ggtcgaggga ggcatgatga
    14161 tggcaattgc cgtggctggg ttgctggcaa atatactttc tttctggtta cttcatcacg
    14221 gcagcgaaga gaaaaacctc aacgtacgag cagcggcact gcatgtgctc ggggacctgc
    14281 tgggttcggt tggggctatt attgccgcgt tgattattat ctggactggc tggacacctg
    14341 ctgaccccat tctctcgata ctggtgtcgc ttctggttct gcgtagcgcg tggcgactgt
    14401 tgaaagatag cgtgaatgaa ttacttgaag gtgcaccggt atcgctggat atcgctgaac
    14461 tgaagcgtcg tatgtgccgg gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat
    14521 ggatggtagg cgagaagccg gtgatgacgt tgcatgtgca ggtgatcccg ccacacgatc
    14581 acgatgcctt gttggatcag atccaacact acctgatgga tcactatcag attgagcaca
    14641 ccaccattca gatggaatat caaccttgtc ataggccaga ctgccacctg aatgaagggg
    14701 tgtctggcca ttcacatcat caccattaat gggacagcgc gcgtgagcct ctttcacgtg
    14761 cgctgttgat ccacatccgg ctgccgttga gcgcaataaa ggtcaggatg atgtactcca
    14821 gagacatggc gtaaacgccc tgaagtgcaa aaataacgac gctaatcacg ttaataatca
    14881 cccacaacag ccagttttcc acatacttac gcgtcatcag aatcattgcc acgatagata
    14941 acaccatcat gcatgaatcc cagaacggga aagcgtccgg ttgcagttca ggcatcacaa
    15001 cctgtaatcc taatgcttgc atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg
    15061 gattgataaa taccgtcatc agaccaatcg aaacaacgca aaccgccaac cagctgagtg
    15121 ccttcggcaa tggcaaccag cgaattttca actccgcctc gttctgactg gtttgtcgcg
    15181 accacgcata ccagccgtaa atattcgcgg caaagaaaaa cacctgtaat agcaggctgg
    15241 catacagctg aatctgaaag aaaataatgc caaacaaggt aacgttgatc aggccaaaga
    15301 agtagttgct gatcttctcc agactggcaa ggccaataca cagcaacccg gcgatcgtgc
    15361 ctaccgcttc gatccatgag agatcataac cgcccgcccc tattggtata tgtaccagga
    15421 tattctgcac actaaaaaaa tccatctttc ccccaaagcg taacgttacg ccttattatt
    15481 cgttatccac gtagtgtagc cgcaaaatcc agcatacgat tgagcggcac cagcgccctc
    15541 tctcgcagcc tctcatcgac atgaacctcg tgattacttc cttcctgttc taatgcctct
    15601 gcgatagcct gaaggtcatt catggccatc cacggacaat gcgcgcagct gcggcaggtt
    15661 gcgccctcac ccgcggttgg tgcttccagt aactctttat ctggcaccgc ctgctgcatt
    15721 ttgtagaaaa taccccgatc ggttgccaca ataagcctct gatgaggcaa tgcttttgca
    15781 gcagcgatca gttgactggt ggaaccgacc gcatccgcca tatcgacaat agcttgtggt
    15841 gattctggat gcaccagtat ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc
    15901 tgagtcttaa attcatcatg cacaatgcag gcaccctgcc agcataaaat gtctccaccc
    15961 gtctgttttt gcacgtaacg ccccagatgt ttgtcgggtg cccagatgat tttttcaccc
    16021 aaactatcaa gatgatcaat aagttcgacg gcaatgcttg aagttaccac ccaatctgcg
    16081 cgcgctttta ccgcagcaga agtgttggcg tagacgacga cagtacgatc gggatgggca
    16141 tcgtaaaatg cgttaaattc ttcaacaggg cagccgagat ccagtgaaca ctcaacctga
    16201 agtgtcggca tcagaattgt tttttccgga ctgagaattt tggcggtttc tcccataaat
    16261 ctcaccccag cgactaacaa agtagaagcg ggatgctttg caccgaagcg cgccatttcc
    16321 agagaatcag aaatacagcc accggtttct tctgccagtt gttgaatttc gggatcggta
    16381 tagtagtggg caaccatcac cgcattacgt tcttttagca gacgttttat cttctcgcgg
    16441 taatacgctt tttcatcaat gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc
    16501 gtgtctggat caaacattac gctcatctta ccatctcgtt ttacaggctt aacgttaaaa
    16561 ccgacattag cgtaacattc gctgttatcg gtgtaatttg ttttatatgc taaacaagat
    16621 agtcgaatgg tggggaaaag tcactcgaat tacgcctaat tccaccgacg tagagttgct
    16681 tgcacagtaa gctctgacga tgtcatcagg tttacaaagt gaaattgagg atgcctgaat
    16741 agcaaaaaag cgcctttagg gcgctttttt gcattggtgg gtcgtgcagg attcgaacct
    16801 gcgaccaatt gattaaaagt caactgctct accaactgag ctaacgaccc gagtggtggg
    16861 tgatgacggg atcgaaccgc cgaccccctc cttgtaaggg aggtgctctc ccagctgagc
    16921 taatcacccg atactgcgct ggatactaca aactacttcc accgaaccca ccgtaattag
    16981 tggtgggtcg tgcaggattc gaacctgcga ccaattgatt aaaagtcaac tgctctacca
    17041 actgagctaa cgacccggtg gtgggtgatg acgggatcga accgccgacc ccctccttgt
    17101 aagggaggtg ctctcccagc tgagctaatc acccgacact acgctggata ctacaactaa
    17161 ctatgttagt gggggtcgtg caggatgact cgacttcgtc tcgcccttcg gcgccgttgc
    17221 tgatgcaacg ttatccttca cgttttacta ccgcttccac cttagcgatt ggtgggtcgt
    17281 gcaggattcg aacctgcgac caattgatta aaagtcaact gctctaccaa ctgagctaac
    17341 gacccacttt ttcgttgctt tcggtttgtt tgatatcccg tggcaacggc ggcatatatt
    17401 actgatttca gacttgagcg caacaaaaat ttcgatgcag atcactcaac tgcttatgat
    17461 tcgcacgaca cgaccagaaa taatgcgact tctggtcgtg tgttatgcat tacatcgcgt
    17521 tcagacgttt ttgtgcctgt ttagcgccat cagtaccagg gtatttactg ataacctgct
    17581 ggtatacggc tttcgctttt gcggtgtcac ctttgtcctg catgatgacg ccgactttaa
    17641 acatcgcatc agcagccttt ggtgacttcg gatagttttt cactaccgaa gcaaaatagt
    17701 acgccgcatc atctttttta cccttgttgt agtttaactg acccagccaa taattggcgt
    17761 ttggcaggta agttgaatca gggtaatttt tgatgaaatt ctgaaatgcc accattgcgt
    17821 catcctggcg ggatttatcc tgcaccagcg caatagctgc attgtaatcc gtgtttgcat
    17881 cgccgctttt caccggcgcg ccagcattcg cagtaccagc atcagctgtc ggcgtcgttg
    17941 atgccgtcgc accgctttga tcgctgctgg ttgattgcgc cgctgcacca ccgctgctga
    18001 ggctgtcgat ctgcaacagg atctgctttt gccgctccac gacctgattc agttgatact
    18061 gattttcctg aatctgacca cgcagggaat caatatcgga ttgattatca gaaagttgtt
    18121 gctggagttg ggttaaaagc tggctgtgag cattagaaat acgctcaagt tgagtgacgc
    18181 ggtcttcgac cgagcctgag ctgacactac tgattggtgc ctgagcaaaa gcggcccagg
    18241 gggccgctat accaaccagt aacgacagac tcaatagttg atgtctgaag ttactgctca
    18301 tgcaattctc ttagtaaacc agtaccgcac gacggttttt ggagtatgcc gcttcgtcat
    18361 gacccagtac tgcaggtttt tctttaccgt aagaaacgat ggagatctgg tctgcagaaa
    18421 cgcctttacc ctgcaggtac atcttaacgg cgttcgcacg acgttcaccc agggagatgt
    18481 tgtattccgg agtaccacgt tcgtccgcgt gaccttctac ggtgactttg taagacgggt
    18541 tgctacgcag gaagtttgca tgtgcatcca gcatttgagc gaagtcagaa cggatatcgt
    18601 acttgtccag atcgaagtaa acgatgttgt tctgctgcag ctgttgcatt tgcagacgag
    18661 cctgctcttc ggaagacatg ttgccgttgc cgccgttcgc atccatacca gtgccggcac
    18721 ccagcatgcc ttcgctgccg tcattgctgg cgttcttgtt ggaagaacat gccgcaattg
    18781 ccataacagg cagagcaatc atcagccctt tcagcacttt gttcagttgc atttcaatga
    18841 ttcctttact attcaattaa ttattatcac agatacggcg accaggcagg gaatttgacc
    18901 tgtccatcag ttgccggaag acgcgctttg aaacgcccat ctgtagaaac caaattcagc
    18961 acggatccca tcccctgaga agagctgtag attaccatag tgccgttagg tgccagactt
    19021 ggcgtttcat ccaggaacgt ggacgacaga acttgtacgc ctcccgttgc cagatcttgt
    19081 ttggcaatgt gctgctgccc accattggag ctgaccatta ccataaattt accgtcgctg
    19141 ctgacatccg catcctggtt ctgcgaacct tcccaggtaa tacgttgtgg cgcaccgccg
    19201 ttgatattca ctttataaac ctgcggacga ccggcctggt cagaagtaaa tgccaggttc
    19261 tggctatccg ggaaccaggt cggttcggta ttgttactgc gaccatcagt cacctggcgg
    19321 atctgaccag aagccaaatc cattacgtac aggttcagac taccggtttt cgacaaggcg
    19381 aatgccagtt tgctgccgtc tggcgagaat gcaggtgcac cgttgtgacg cgggaatgaa
    19441 gccacctgac gtacagcgcc atttgccagc gtctgaataa ccagcgcgga acgaccgctt
    19501 tcgaaggtca cataagccag tttagaaccg tctggtgacc acgccggaga catcagcggc
    19561 tgcggtgaac ggtgaacgac aaactggttg taaccgtcat agtcagatac gcgcagttca
    19621 tacgggaact gaccgccgtt ggtctgaaca acgtaggcaa tacgggtacg gaacgcacct
    19681 ttaatgccgg tcagtttttc aaacacttca tcactggcgg tatgaccagc ataacgcagc
    19741 cactgcttgt tcactttgta cgagttctga gcaagtacag tacccggtgc gccgccagtg
    19801 tcaacaagtt gataagcaac attgtaagaa ccatccggat tcggagtgac ttgaccgaca
    19861 actacagcgt caatgcccag tgcggaccat gcagctggtt gtacttcctg cgcactaccc
    19921 ggctgctgtg gcagacgagc gcgatctaac ggattaaatt taccgctgtt acgcaagtca
    19981 gcagcaacga tgccgccaat atcttcaggt gccgcaccag gccccgccca ctggaaagga
    20041 acaacaccaa taggacgacc ggaatctaca ccgctgtcga tcacaatgcg gacttcagca
    20101 tgcagaactg atgcccacag tatgagaaaa ccaaatgcta ctcgtaatgc ctgcttcatc
    20161 atatctccct tatctggacc gatggcccac gataatttag cagaatgtta acaaactaaa
    20221 atacacaaaa ctaccagaac cccgtggcaa ccggtgcctg atgttgaccg tccgaacagt
    20281 caacatcgcg attacggttt gaagtccaat ggcgcgtttt tgaacacttc atatactgcc
    20341 tggcttggtg gtttcgggat cttcgcaagt ttagctgctg ccaacgcagc ctgacaaagt
    20401 gcgggatcgc caccttcagg tttgatatcc agtaacatac catcgggtgc cagttttatg
    20461 cgcagcgtac aggttttgcc tgcataggac gatgcgtcat agaacttact ttcgatagca
    20521 gatttaatct gcccggcata gttattgata tcggcccctg atgcgccatt gtttttagta
    20581 ttaccactcc cggcaggcga agcattgttc cctttcgccc ctccccccgt tttcggtgca
    20641 ttcttaccag agcttagctc accgaaaata tcatctgcct ctgcggcagc ttttgctgca
    20701 gcggcttttt cagctgctgc ttttgccgct gccgcttttt tgtctgcggc ggctttttct
    20761 gccgctgctt tcttatcagc tgctgcctta tcagcagccg ctttttctgc tgcttttttg
    20821 tcggctgcag ctttctctgc tgccgctttc ttatcagctg cagccttttc agcagccgct
    20881 ttcttctcag cttctgcctt ggctttttca gcagcttcag ttgccgcttt ctttcttgct
    20941 tcagctgcag ctgcttctgc cgcttccgct ttcttcttca gtgccgcagc ggctacctcg
    21001 gcttttttct gcgcttcggc tgcggctttg gcggcttctg cttctgcttt tttcttcgcg
    21061 tctgcagccg ctttcttcgc tgcttcttcc gcagctttag catctgccgc cgctttcgct
    21121 gccgccactt cagcttgctt ctgctttaac tcggcctgtt ttgcggcttc ttcagcctgc
    21181 tttttctgct cctgagccgc taaccgctct ttctcaagtt gcttcaggcg ttcctgttca
    21241 gccgcttgtt tctcacgcag ttcttcagca gcctgctgtt ccttcatctt gcgctgttca
    21301 tcagaacgct tcgcgcttga ttcctggctt tgcatgcgtt tgtactgctc aactaccgca
    21361 cctgaatcaa ccatgacagc gtcgatggac gaaccaccgc cgcctccggc tgaagcttct
    21421 atattctcat cgaacgaact ccagatcagc gccgcaaata agatgacatg cagcactgct
    21481 gaaataatta tcgcccgctt gagcttgtcg ttttgttcgg ttgcctttga cactctcggt
    21541 ttccaaaaac tgttcgcctg ttacccgctc tctttcaagc aagggaaacg cagatgttta
    21601 gataggctgc gtcattaaac caaccgattt cacacccgca ctatgtaaca agttcagtgc
    21661 tttaattatt tcatcgtaag gcacatcttt tgcgccaccg atcagaaaga ccgttttcgg
    21721 gttggccttg aaacggctgg acacttccgc caccacctgc tctggtggta aacgctccag
    21781 gcgatctttc tcaaccacca cggtgtactg accaatacca gacacttcaa caatcactgg
    21841 cggattatcg ttactgctca ccgcctgtga ttcagtagcg tctggcagat cgacctccac
    21901 gctctgggtg atgatgggcg ctgtcgccat aaagatcagc aacagcacca gcagtacgtc
    21961 cagcaacgga acaatgttga tttcggactt gagatcgcga cgacctcgtc cacgcgctct
    22021 ggccatggct taccccttgt tgctctcgct aacggtaaac gcctggcggt gcagaatcgc
    22081 ggtaaactct tccataaagt tgtcgtaatt cagttccagt ttgtttacgc gctggttgag
    22141 acggttataa gccattaccg ccggaattgc ggcgaacaga ccaattgccg tcgcaatcaa
    22201 cgcttctgcg ataccgggcg caaccatttg cagtgttgct tgttttaccg ccccgagggc
    22261 gataaaggcg tgcatgatcc cccagaccgt accaaacaga ccaatatacg ggctgatgga
    22321 gccaaccgta ccgaggaacg gaatgtgcgt ttccagattt tcaagttcac ggttcatgga
    22381 gatacgcata gcacgcgacg ccccttccac tacggcttcc ggcgcatggc tattggcacg
    22441 atgcaggcgc acaaactctt tgaacccgct gtaaaagatt tgttccgaac cagtcagatt
    22501 atcccgtttc ccctgactct cttgatagag gcgagaaagt tcgattccag accagaattt
    22561 atcttcaaac gcttcggctt cacgcgccgc tgcgttaaga atacgggttc gctggataat
    22621 aatggcccag gatgcgattg aaaaaccaat caaaatcaac atgataagtt taaccagaag
    22681 gctagccttc aggaacaaat caaggatatt catgtcagtc actgcttaaa ctccgcgaca
    22741 atagacttgg gaagcgcacg aggcttcatt ttgagtgggt caacgcaaac aaccagaacc
    22801 tctgcttcat tcagcaaagt attctcggcg ttgacaatac gttgcgtgaa aaccaaagag
    22861 gtgccacgca ttgatgttat ttcagtctgt atttcgagca tatcgtcgag ccgcgcaggt
    22921 gcgtaatatt ccaccgtcat tttacgtacc acaaaggcaa cgcgttcagc catcagcgcc
    22981 tgctgactga agtgatgatg acgcagcatc tctgtgcgtg ctctttcata aaaagcgacg
    23041 taactggcgt ggtacaccac accaccggca tcggtatctt catagtagac gcgaaccggc
    23101 catcgaaaca gcgttgtatt cactttacat cccggtaatg caacaaaagt tagagctttt
    23161 aaacgctgct actatacgcg cgcgattgag gtttgggaat gagggcaagt taagggcgcg
    23221 taaaaattta tgggcctaga gcggcccata attttttaaa gagaattctg atttaaaaga
    23281 agaagaaaat cagcccgaca atgagaacaa tatcggcaag caatgggcaa aaaatccctt
    23341 gccaaagaac cttctgcgga cgaaagccca cgccgtgaat cacaccagca cagacggccc
    23401 acatcagcaa taaaccatgc cagatttcca gttcactggt cttcgcggca aaacgagatg
    23461 ggtcccaaaa catacatcct gctaacagaa gcgccatcac gaaggaaagc gcccgcaggg
    23521 ggcgcttgtc cattaccgca tacaaggtcg cgataatctt actcatcaga tgtcttcttg
    23581 accggctttg cctgattcga cgtgttcaag cgccagcgcg gtgattaccc caaacgaaca
    23641 ggcaagaagc gttcccagaa tccatgcgaa ataccacatt tttagctcct tacttagtac
    23701 agagagtggg tgttacgttc aatatcttct ttggtgatac gaccgaacat tttccagtaa
    23761 caccaggcgg tgtagagcag aatgatcggt accagaacca ccgcaaccca ggtcatgacg
    23821 ttaagcgtca gctggctgga agttgcatcc cacattgtca gacttgcgtt catcatggtg
    23881 ctggacggca tcacaaacgg gaacattgcg ataccggctg tcaggatgat gcaggccagc
    23941 gtcagggagg agaacacaaa cgcccacgcg gctttatcca tacgtgcagt caggatggtc
    24001 agcagcagca gaaccacacc cagtgccgga atagcccaca gaattggcgt gttgttgaag
    24061 ttaaccagcc atgcgccagc ttcacgaacc acttctttat tcagtgggtt agaggctgcg
    24121 taatggtcca ttgtcgattt cacgacataa ccatcgatac cgtacatcac ccatacgcca
    24181 gccagtgcga aacagaccag tgtcaccagc gcagccacct gagccgttgc acgggtacgc
    24241 aggtgcagtt cgcccacggt acgcatttgc agataggttg cgccctgagt aatgatcatc
    24301 cctacgctca ccacgcctgc cagcaggccg aacgggttaa gcaactggaa gaagttaccg
    24361 gtgtagtaca gacgcagata ttcatcaacg ttgaacggta cgccctgcaa caggttaccg
    24421 aacgctacac caattaccag cggcggaacg aagctaccaa tgaagatgcc ccagtcccac
    24481 atgttacgcc agcgggtttc ttcaatcttg gagcggtagt caaaaccgac cggacggaag
    24541 aacaaagacg ccagcacgag gatcatcgcc acatagaagc cggagaacgc agcggcatag
    24601 accatcggcc aggcagcaaa gagtgcgccg cccgcggtga tcagccaaac ctggttaccg
    24661 tcccagtgtg gtgcaatgga gttaatcata attcgacgct cggtgtcgtt acgaccgagg
    24721 aaacgggtga gcatgcccac ccccatgtcg aaaccgtcag tgactgcaaa accaatcagc
    24781 agaacgccaa ccagcagcca ccagataaaa cgcaatactt cataatcgat catttgacga
    24841 ctcctgtctt agcgtgccgg ctgagtagtc gtggaagact gctcaaagtg atagcgaccg
    24901 gttttcaggc tgcttgggcc gaggcgtgca aacttgaaca ttaagaacaa ttctgccacc
    24961 aggaacaggg tatacaggcc gcaaatcagc accattgaga agatgagatc gcctgcggtc
    25021 agtgacgaat tcgccacagc tgtcggcagc acttcaccga tagcccacgg ttggcggcca
    25081 tattcagcca cgaaccagcc cgcttctaca gcaatccacg gcagcggaat accgtacagc
    25141 gcggcgcgca gaagccattt tttctcgcca atgcggttgc ggatgacact ccagaaagag
    25201 agcgcgatga ttgccagcag caggaagcca cacgccacca tgatacggaa cgcaaagtac
    25261 agcggcgcta cacgcgggat ggagtctttg gttgcctgtt gaatctgcgc ttcagtcgca
    25321 tcagccacgt ttggcgtata gcgtttcagc agcagaccgt aaccgaggtc tttcttcatg
    25381 ctattgaact ggtcacgaac cgtctggtcg gtagaaccag aacgcagttg ttcgagcaga
    25441 gagtacgcct tcatcccgtt acgaatgcgt tcttcatgct gcaccatcag ctctttcagg
    25501 ccgataaccg gggtatccac ggaacgcgtt gcaatgatgc ccagtgcgta agggatctga
    25561 atcgcaaatt tgttcgtctc ctcttcctga tcaggaatgc cgaacaaagt aaaggcagca
    25621 ggcgcaggtt gcgtttccca ctcggcttca atagcagcca gtttggtttt ctgcacgtcg
    25681 cccatttcgt agccggattc atcacccaga acaataacag acagaacagc agccataccg
    25741 aagctggcag cgatagcaaa ggagcgttta gcgaaggcga agtcacgacc tttcagcata
    25801 taccatgcgc tgataccgag gatgaacatc gcgccagtca cataaccaga cgctacagtg
    25861 tgaacgaatt tcacctgagc aaccgggtta agcaccagct cggagaagct caccatctcc
    25921 atacgcatag tttcaaagtt gaaatcggac gcgattgggt tttgcatcca gccgttcgca
    25981 accagaatcc acagtgcgga caggtttgaa ccgagcgcca ccagccaggt gacacacata
    26041 tgctgaactt tacccagacg atcccaaccg aagaagaaca gacctacaaa ggtggattcg
    26101 aggaagaagg ccatcagacc ttcgattgcc agcggcgcac cgaagatatc ccctacatag
    26161 tgggaatagt aagaccagtt agtcccgaac tggaactcca tggtcagacc ggtagccaca
    26221 cccagagcga agttgatacc aaacaacttg ccccagaact tggtcatatc tttataaatc
    26281 tgtttgccgg agaggacgta gaccgtttcc ataatggcca ggaggaacgc cataccgagc
    26341 gtcagtggca caaaaaggaa gtggtacatc gcggtcaagg caaactgtaa gcgcgacagt
    26401 tcgactatat ctaacatcat gactccttgc tcatcgcatg aagactccga gagtgaaccc
    26461 cgttagaaag ggtcacacgc atgccccaat agaaatttgt ttgaacactt tcatcatacc
    26521 atgtgaatgc acacaaaaac ggcgtaaaga aaggttgcaa acatgttaat aaaaactcaa
    26581 attgatccca cgtatatatt acgccgcaaa atccttacaa taaacaggtt tttgttgaag
    26641 caaatttgca tttttcgacg gtgatcaatt tatagctaaa ttgccgcctt tcagccaatt
    26701 tgatcgagaa caatttatct ctttttgatg cccatttcca agacttatac attgataaat
    26761 atcaatttct acaaattatg ttaatcatgt ttcaacaagg taactttagt gatatttatg
    26821 tttaaaatgt gtgaattata agactgaaat tagtttcggt tggcaattaa agataaaaag
    26881 agacgaagca aaaacagcac ggttgacctt aattcttttt ttggtcactg gctaatacga
    26941 cgcagagttt caggattatt aataaaggca taaatctccc tcccgccgat attaacattt
    27001 aattaactta taattatatt ataattaact cacccgtggt tattattttg ttaaaaataa
    27061 agccacatat acgtagcttg agagtgaaga taaacacaaa attacctgat gcgctacgct
    27121 tgccagtgtc tacatcatct ctgcaatata ttgaatttgc ctgcttttgt agaccgaata
    27181 aagcgttcat gccgtatccg gcatgaacaa agcgcccttt gtcaacaatc tggccgcgca
    27241 tatgtggctt cggtttgatt gctattcagg caagccggta actgaacgtc cgtgactggc
    27301 cgggtaaaaa aggccccgat agatttgaac cttgattttc ctcggtggta atgtgttcat
    27361 ccatcatcgt ttctgagcaa gaaatcacct cgcgactgaa agcaacagtc gcatcacagg
    27421 ttgctgattc agccggatta aacagccgta aaatcacttc ttgtcggtct tcagctttct
    27481 taagtgcgct tatcaggggc atcccactgg gggcattttc aacaaactat aactttccgg
    27541 cacgttgaat ccggctttgt tgagcttcat cgcatcccat gggattttgt tgtaacactg
    27601 tactggagtc agccatgctc gcgcctgctg agctacacca gcggcggttg gcgtaccggt
    27661 ataactcaat aaacttaggc gacaagaaag cagaccacgt agttgtgagt ccgggactgg
    27721 cattttaatt cccgaaggcc gcctaggcct taaaagcaga tcttctttcc ccagtaagcc
    27781 cacgccgcgc agcaacgtaa tggcaaaggt tttcttctct tcaccgatga cttcaaattc
    27841 acgtagccct tcgctaaaga cagccatgcc gttacgccct tcctgtaagg ctgcatagtt
    27901 gagcatattc cataccggaa ccggcgcttc tttccagctt tcttgctgcc agttgttcat
    27961 tgcactgtcg ttcacggggc gcgttagcga accaaactgg gtatctgcca gaacactatc
    28021 ggtgttaaaa gatgtaggga tcaggacgcg aaggcgatga tcgtcagcct ggttatcaag
    28081 gttgatatcc acatcaatgc gcctgttgtt atgactaaga gtgacaacca actcgacatc
    28141 tactctgcca gtggattgtc ttgcgctgcg ttgttgcaga ttacgcggca ccgccatttc
    28201 atagcggata acagccctgc tctgccaggc ctcatgaata atctcgcatt gcggtttcgc
    28261 attcgccgaa gtaattaccc actcttcttt tgcgggtgaa tagtcatatt catcaccatc
    28321 atcagagctt tcttcaattt gcaacacccg gtcatagcgc acaccgctat ctttatctac
    28381 cagttgtagc gttccatcct cattgagcgc aatttgccag aaagcatttt ccagtatccc
    28441 ttcagcgtca ctttttgccg caatcacgtt gccaggctga ttcgcttcga tataaagcgt
    28501 gcgatagccc atagaaggga caatctggtt gatctgtata tcaaactcca taaagggatc
    28561 gtaattaccg taatgaacta tttgccgatc gattaggcct ggatcgatct cacgggcatg
    28621 gcgaataaaa tacggtacag gctgaccgcg atcgtctcgt agattaaact ggctggcgcg
    28681 cagccgcaca gtggtgttga tcacttcttc ccgcggccag ggcatcaggt taaacaggac
    28741 gagtttgtcg gcgtcgctct gcggcatgtt gtcggcgatt ttacgcatat agaaacgcag
    28801 cagattatcc gccatgtctt cagccagttc gaagcgggcg acgatttcgc gatgaacttt
    28861 gtcacgacag cagcagccga tgctgtcgtg ggcatgattt tttaagatct ctttccacat
    28921 tttctccagc aagccgtggt ggtatccaaa acccaacgtc ctggccagcg ttgccagcgg
    28981 ttccagcaga ttaacaatct tattttcaat acgtgcgtgg gcaactttga tatccatgcg
    29041 cgtggaaccg atggtgcgat gcacgcgcat atatttgcca tcaataaatt cccctttcag
    29101 ggttgccaga ttatctcgct gcgcttcgat cttctcaaat acctcttcaa agcggctcat
    29161 cacaaacttt cgttgaggat aaatttcgcg cagtttctcc atcacttcga agatattttg
    29221 ctgcaatggc atctgatcat gcccattcgg cagcaaaatc tctttggtta cagacgcttt
    29281 ttccagcacg tcaaaataac tgtcgaggcg tttgcgtaat ccgttttcgt ccgcaggtaa
    29341 gtacttaccg atggcataac ccaacggcat cacctgcgcc gtcacttcgc taccgtcact
    29401 gctttgccac aaaaactcgg ttttatccgt accgtggcgc tcagaacatc cacgccagaa
    29461 catggtgcag gtaatgccaa atccattgta gatatgcggc agttgcccgg acatgccaaa
    29521 ggaatcgggt aaataaccta ttttcatcgg ctcgccaaac gcgaggcagt cacgcattcc
    29581 gtacatcaga ttacggacga tggattccgc agaaacaatc gtggtatcgg tctgggtata
    29641 ccaggggccg ataatcaact tgccggcttc taccagtttc ttcaagcggt ctttgttttc
    29701 cggtttcacc gcgaaataat cttcgaggat cgccgtttgc ccgtcgagta cgtaatattt
    29761 gtattcgttg tcctgttcca gtcggcacag gatctcttcc atattattga ccagcagaat
    29821 gcgtgactct tcggtggtga aataccactc ccgatcccag tgcatatgcg gggtgatgtg
    29881 aacgcgagat accgctttca tcgtcgtttc ctttttgttt atggcattac gccatcagtc
    29941 agatagttgc catgcttaac cgcgtgacgc cgccagatca ggagaattgc agtcgagatt
    30001 gcagccccca ccaatgccgc accaaaccag ccaattgccg ccataacacc gcccgcgcca
    30061 ttatcatgaa gtaaaaagag cgagaaaatg ccagcaccgg gtgtcgaaag gccgatattc
    30121 atcgcaccga caatagcgcc cgttaccata gagcccagca caaacgaacc aataacccgc
    30181 agcggatctt caatcgccat cgggatcgcc ccttcggtaa tacctgccag ccctaacagc
    30241 caggtggatt tcccggtctc aatttcaaac tctttaaaca ggcgcggtgc gagcatcgtg
    30301 gaagcggtta cggtaaatgc cgaaaccatt ttgacggagg cgaaaatggc atacgggccg
    30361 taaacgccgt tcgccattgc gcccaggcag aatgcataag cggctttatt cactggccct
    30421 ccaaggtcaa aggaacacat aaaaccgaga atggcaccca gcaacagcgc gttacttcct
    30481 gacagaccgt tcagccaggc ggtaagcgag ttattgatcc aggcgacagg ttcccccacc
    30541 acaaacagca tcagactgcc cgctcccaat gtaccgagca ccgggtagag ataaaaagtc
    30601 aggaagccat tgaatttact gctaagacgc aagtgatttt tcatccagcg tatcaagtaa
    30661 ccggctatca atccgccaac gaccgcgccg agaaacccgg agccgatcat gttggcggca
    30721 agtccagccg caaagcctgg cgctaacgcc ggtttatctg ccagcgaata ggcggtatag
    30781 gctgccagca ccggtaccat caaaattccg agcagcccgc cgcccagttt gcggtacatc
    30841 cacagccagg agttttcttc attaaacaga tcttgtagcc cgaagatttg cgacagtaat
    30901 accgcgaccg ccagcaccgt cccccccgcg acaatcaacg gtacggcaaa agagatcccg
    30961 ctcaacagtg cctgtttcag ctccgttttg acgcttttca ccggttgcgt atcttgctgt
    31021 aaggtacgcg tctcatcgcc acgctcgagg gtaagcgatt gttggatcaa cgcttctgca
    31081 tggcgaatcg gctcggcaac aggcacagaa agcgcaggaa tgccgttaaa acgctcactc
    31141 tccttgatgg cgacttcagc cgcaaaaata caggcagttg cactattgag ttgatccgcc
    31201 gttaaacgcc cttcaatacc gttagcacct tgcttttcaa cgtagacatt cacgccgagt
    31261 ttgcgtccgg ctttttccag atactccgca gccatgtagg tgtgagcaat acccgccggg
    31321 caggccgtta cgcaaacgat agttggcgcg ttggaaaaag aggcagaagg ttgcgtgcct
    31381 cccttgtcat ccagcgccga gagcaattca tcaggcgtcg tcgccgactg tatacgcgcc
    31441 cgaatctcat catccgccag gcgcgtggtc agcgctgtca gcagttgcat gtgcgtagta
    31501 cccgcttcat tgaggggaat cgccagcaac accactaaat caaccgcttc cgggccatcc
    31561 acgccttccc actgaagcgg ctcgctgagt gtggcgactg caaacgccgc ttcttttacc
    31621 gcagcagttt tgccatgcgg cacagccaac ccttcactta aggcggtcgg gccaaggctt
    31681 tcacggcgat acacttcttt cagaaattgc tcagtactgg aaattttccc cagagcagca
    31741 agacgttgag tcaacgcgtg aatggcctct tcacggctgg taaagcgcgc attcagacac
    31801 aacgcatcgc ggtgggtcag agtcgtcagg ttcatcatga tcctcacttg atcgttttta
    31861 tagtcgcgcc agtgtgcccg acaaaacaat accattgcgt aatcaatttc acttgattga
    31921 aattatttgc attttatttg tattgtttac aggatgagtt tatcgggcat aatggctaaa
    31981 ttcgtattaa tgagatgcta aataatggga cacaaaccct tataccgaca gattgctgat
    32041 cgcattcgtg agcaaattgc gcgtggcgag cttaaacctg gcgatgcgtt acctaccgaa
    32101 tcggcattac aaacagagtt tggcgtcagc cgggttacgg tgcgtcaggc tttgcgccag
    32161 ttagtcgagc aacagatcct cgaaagcatt cagggcagcg ggacttacgt caaagaagag
    32221 cgggtcaatt acgatatttt tcagttaacc agttttgatg aaaaactgtc ggaccgtcac
    32281 gtcgatacac acagtgaagt tctgatattt gaagtgattc cggctgacga ttttcttcag
    32341 caacagctac aaatcaccgt gcaggatcgt gtttggcatg tgaagcgcgt gcgctatcgc
    32401 aagcaaaagc caatggcgct ggaagaaacc tggatgccgc ttgcgttgtt cccggatctc
    32461 acctggcagg tcatggaaaa ttcgaaatat cactttattg aagaagtgaa gaagatggtt
    32521 atcgatcgta gcgaacagga aatcattcct ctgatgccaa ccgaagagat gagccgtctg
    32581 cttaatatca gccagacaaa gccgattctt gaaaaagtat cgcgtggata tttagttgat
    32641 ggtcgggtgt ttgaatatag ccgcaacgct tttaataccg atgactataa atttaccctg
    32701 atagcgcagc gaaaatcatc gcgataagca caaaaaggcc atcatacgat ggccaaccat
    32761 gtcgaaaccg acagcccttc gccattcctg tagagggaag cggcgagggc tatttcttat
    32821 tacagatatt tatttcagaa cagttttcag tgcttcaccg atatccgcca ggctgcgaac
    32881 ggttttcacg cctgcggctt ccagagcagc gaatttctca tccgcagtcc ctttcccacc
    32941 ggcaatgatc gcacccgcgt ggcccatacg tttgcctttc ggcgcagtca caccagcgat
    33001 gtaacccaca actggcttgg taacatgctc tttgatgtac gctgccgctt cttcttcagc
    33061 gctaccgccg atctcaccga tcatcacgat cgcttcggtc tgcggatctt tttcgaacat
    33121 ttcgagaatg tcgataaagt tagagccagg gatcgggtca ccgccgatac cgacacaggt
    33181 cgactgaccg aaaccgtaat ccgtggtctg tttaaccgct tcataggtca gtgtaccgga
    33241 acgggaaacg atacccactt tacccggttt gtgaatgtga cccggctgga taccaatttt
    33301 gcattcaccc ggggtgataa cgcctgggca gttcgggccg atcatacgaa cgcctgcttc
    33361 gtccagtttc actttcacgg tcagcatatc cagcgtcggg atgccttcag tgatggtgat
    33421 aatcagtttg atgcctgcat cgatagcttc cagaatggag tctttgcaga acggtgctgg
    33481 tacgtaaata acagacgcgg aagcgccagt agcagcaaca gcttcacgca cggtgttgaa
    33541 caccggcagg ccgaggtggg tagtgccgcc tttacctgga gttacgccac caaccatttt
    33601 agtgccgtat gcaatggcct gttctgagtg gaaagtcccc tggctaccgg taaagccctg
    33661 gcagataacc ttggtgtttt tatcaattaa aatggacatt atttcccctc cactgcggca
    33721 acaacctgct gagctgcatc cgtcagacct tttgctgcaa taatattcag gccgctgtca
    33781 gccagtttct tcgcgccgag ttcggcgttg ttaccttcca gacgtaccac gaccggtacg
    33841 ttaacaccca cttccgctac cgcgccgatg ataccgtcag cgatcaggtc gcaacgaacg
    33901 ataccgccga agatgttaac cagaacggct ttcactttgt cgtcagagag gatgattttg
    33961 aacgcttcgg ttacacgttc tttggttgcg ccgccgccaa cgtccaggaa gttagccggt
    34021 tcgccgccgt gcagtttaac tatgtccatc gtacccatcg ccagacctgc gccgttaacc
    34081 atacaaccga tgttaccgtc cagcgcaacg tagttcagtt cccactgtgc agcctgtgct
    34141 tcacgcggat cttcctgcga ctggtcacgc atttcgcgca gatcaggctg gcggaacagg
    34201 gcgttgccat cagcgcccag tttgccgtcg aggcaaatca gatcgccctg tttggtgatg
    34261 accagcgggt tgatttcgat cagcgccagg tcgcgctcca ggaaaatggt cgccaggccc
    34321 atgaagattt tggtgaactg ctgaaccagt ttaccttcca gacccagttt gaacgccagc
    34381 tcgcgtccct gatacggcat cgggccagtc agcggatcaa gcgcaacttt atggatcagg
    34441 tgcggagttt cttccgccac tttttcgatt tccacgccgc cttcggtgga ggccataaag
    34501 accacgcgac gggaactacg gtctacaacg gcaccgagat acagctcttt agcgatatcg
    34561 gtcgccgctt caaccagaat ctggttaacc ggttggccat tggcatctgt ttgatacgtt
    34621 accagacgct tgcccagcca gttttctgca aaagcacgga tgtcttcttt gctgtttaca
    34681 actttcacac cgcccgcttt accgcggcca ccagcgtgaa cctgacattt cactacccac
    34741 ggaccggcac cgatttttga agcggcttct tctgcttcgc gcggagtagt acaggcataa
    34801 cccaccggtg ctggtaagcc atagcgggca aacagttgtt ttgcctgata ttcatgtaag
    34861 ttcatgtgtt ctgtccatcc ttcagtaatc gttatctttt aaaccgtaga ccgacaccct
    34921 atccacgtcg gtctacagtg caggtgaagc ttaaactact acacgtccag cagcagacgc
    34981 gtcggctctt ccagtaactc tttgatcgtt accaggaagc ccacggattc gcgaccatcg
    35041 atcagacggt gatcgtagga cagcgccagg tacatcatcg gcaggatctc aacctgacca
    35101 ttcaccgcca tcggacgatc tttgatagcg tgcataccca gaattgcgct ctgcggcggg
    35161 ttgatgatcg gcgtagacat cagggaaccg aacacaccac cgttggtgat ggtgaagtta
    35221 ccaccggtca gatcttcaac cgtcagcttg ccatcacggc ctttgactgc cagctctttg
    35281 attttcttct cgatgtctgc catgccgagg gtatcgacat cacgcagaac cggcgtcacc
    35341 aggccgcgcg gcgtagaaac cgccatgctg acgtcgaaat agttgtggta aaccacgtca
    35401 tcgccgtcga tagacgcgtt cacttccggg taacgtttca gggcttcaac caccgctttc
    35461 acgtagaagg acataaagcc cagacggatg ccgtggcgtt tttcaaacgc ttcaccgtac
    35521 tgcttacgca gatccataat cggcttcatg ttgacttcgt tgaacgtggt cagcatggcg
    35581 gtggagtttt tcgcttccag cagacgctct gccacacgct tacgcaggcg agtcatcggg
    35641 acacgttttt cactacgtgc agccagagcc ggttgcgccg ccggagcagc cgctgccgga
    35701 gcagactctt tcgccggggc tttcgccaga tgtttttcca catcttcacg agtcagacga
    35761 ccacccacac cggtgccttt aatggcgctg gcgtcgagat tgtgttcagc cagcaggcga
    35821 cggatcgccg ggcttaacgc atcgttgttt tgctcttcca gagacgcctg ctggcgttgc
    35881 gccggagtgg acgctttctc ttcagatttg gcgctggttt ctttaccggc gctgttgcct
    35941 tcacgcaggc gaccaaggat ctgacgagac gttaccgttg taccttcatc ttccagaacc
    36001 gcatccagaa tgccgtctgc tgatgccggt acttccagta ccactttgtc agtttcgatt
    36061 tctaccagca cttcatcacg tacgactgcg tcgccgggtt ttttatgcca ggttgcgacg
    36121 gtggcatcgg ctacggattc aggcaggtca gggaccagaa tatctacgct actcattgtg
    36181 tatcctttat ttattcgacg ttcagcgcgt cattaaccag atcttgttgc tgtttctggt
    36241 gaacggacat ataccctacc gccggagagg cggaggccgg gcggcctgca taacgcagag
    36301 aagccccaaa cggaatcact tcacggaaat gatgctggct gcagtaccat gcgccctggt
    36361 tgagcggctc ttcctggcac cagacaaaat ccttgacgtg agcaaactgc tgcaacactt
    36421 cctgcatcgc tttatgcggg aacgggtaga gttgctcgat acgcacaatg gcgacatcgt
    36481 gttgattgtt cttacgacgc tgttccagca ggtcgtaata aaccttacca gaacacatca
    36541 ctacgcgctt cacgcccttc ggatcaagct cgtcgatttc accgatggct ggcaggaagg
    36601 tgccgttcgc cagttcttcg aggctggaaa ccgccagcgg atgacgcagc agggatttcg
    36661 gcgacatcac gaccagcgga cgacgcatcc cgcgcagcgc ctgacgacgc agcatgtggt
    36721 aaacctgtgc cggggtagac ggtacgcaaa cctgcatgtt ttgctcagca caaagttgca
    36781 gataacgttc cagacgcgcg gaggagtgct ccggcccctg cccttcgtaa ccgtgcggca
    36841 gcaacatcac cagaccacac atccggcccc atttctgctc gccagaggag atgaactggt
    36901 cgataaccac ctgcgcaccg ttggcgaagt caccgaactg cgcttcccag atggtcaggg
    36961 tgcgtggttc tgcggtggca taaccatatt caaacgccag cacggcttct tctgacagta
    37021 cggagtccca gacacggaac gcgccctgac cgttatggat gtgttgcagc ggcgtgtaag
    37081 tggaaccgtt agactggttg tggatcaccg cgtggcggtg gaagaaggta ccgcgaccgg
    37141 agtcttcacc cgacaggcga accggaatgc cttcatcaac cagcgtggcg taagcgaggt
    37201 tttccgcacc gccccagtcg aacagtttct caccggctgc catcgcctgg cgatcgccat
    37261 aaatcttggc aacgcgagac tgcatttcaa ctgcttctgg taccgtactg atacgtttag
    37321 ccagttcctg caggcgcttc atctcaactt tgttcgggta ctcttcgtcc cattcgtggt
    37381 tgaggtacgg cgaccaggtg aaagagtgca tgttcatcgg acgccactct gccactacgc
    37441 aatcgccagc atccagcgca tcgcggtaca ggttaaccat ctcggtggca tcttccagcg
    37501 tcgccacttt ttcctgctcc agcttgtcag cgtagatttt gcgcggtgtc ggatgttttt
    37561 tgattttctg atacatcagt ggctgggttg cgctcggctc gtcggcttcg ttgtggccgt
    37621 gacggcggta gcataccagg tcgatgaaga catcacgttt aaaggtgtta cggaaatcga
    37681 gcgccagacg ggtcacaaag gcaacggctt ccggatcatc cgcgttaacg tggaaaatcg
    37741 gtgcctgaac catcttaccg atatcagtac agtacggtgt agaacgggca tccagcgggt
    37801 tagaggtggt gaaaccaacc tggttgttga taacgatacg taccgtaccg ccaacttcat
    37861 aaccacgcgc tttcgacatg ttcagagttt cctgaaccac gccctgcccg gtcactgcgg
    37921 cgtcaccgtg gatggtgatt ggcagcactt tgttgctgtt cggctcatca agtctgtcca
    37981 gacgggcacg aacagagccg ataacgaccg ggcttacaat ctcaaggtga gacgggttaa
    38041 acgccagcgc caggtgcacc aggccgccat cggtctggaa gtcagacgag aagcccatgt
    38101 ggtatttcac gtcgcctgtg ccgaggtgtt ctttatgttt accggcgaac tcgtcgaaca
    38161 agtcttgcgg ttttttaccc agcacgttca ccagcacgtt cagacgacca cggtgcgcca
    38221 tcccgagaac cacttcgcgg gtgccgctgt tgccagcgtg gcggatcatc tctttaagca
    38281 tcgggattaa cgcgtcaccg ccttccagcg agaagcgttt tgcgccaggg aattttgcgc
    38341 cgaggtaacg ctcaaggcct tcagcggcgg tcagttcgct taagaagcgt tttttctctt
    38401 cgctattgaa agtcgcgcga ccagactcga tacgctgttg gatccagcgt ttttcttcgg
    38461 tgctggtgat gtgcatatac tcggcaccaa tcgggccgca gtaggtttgc ttgagggcgg
    38521 aaataagctc gccgagtttc atggtttctt tgccgctggc aaatgaaccg acgttgaagg
    38581 tttcctggaa gtctgcttcg gtcagatcgt ggaaagacgg atccagatcg gccactttat
    38641 cttgctgcca cagtcccagc ggatctagat tcgcatgctg gtgaccacgg aagcggtatg
    38701 cgttaatgag ctgcaggact ttaacctgct tcacattggt gtcagggtcg gagatcgttg
    38761 aagagtaacg tgaagcgtct ttcgccaggc ggcggaaata ttcacgcgtt tgagagtgga
    38821 attgatccgg tttgactccc gtaccaggta actgctggaa cgtcgaacgc cagttagcgt
    38881 caaccgagtc aggatcggtt aagaagtctt catagagctg ttctatccag ctctggtttg
    38941 cgccagagag gtaagaagag tccaaccagg ctttcaaagc gctgttctgc atcgtgatcc
    39001 cttaagcatc ttttttatgc ttacttcgcc gtggatacta ccacgcacag tgatgtgcgt
    39061 gccggggttc acttgcgagc tcttgtctac gtagtggctc gcgaaggaac ctttaaaaac
    39121 tgtcttagtg tcaggggcag tttttaaaga tttcctgcat ttctttgtta cgcctgatgc
    39181 gctgcgctta tcaggcctgc gggtaatgac ttgtaggccg gataaggcgt tcacgccgca
    39241 tccggcactg gttgcctgat gcaacgcttg cgcgtcttat caggcctaca gtttacgcat
    39301 tacgttgcaa caacatcgac ttgatatggc cgatggcgcg cgtcgggttc agccccttcg
    39361 gacatacact gacgcagttc atgatgctgt gacagcggaa tacgctgaat gcatcgctca
    39421 aaccgtcgag gcggctgtca gtctcggtat cacggctatc gatcaggaaa cgatatgccg
    39481 ctaacaagcc tgccgggccg ataaacttat cgggattcca ccagaaagac ggacaagagg
    39541 ttgaacaaca tgcgcagaga atacattcat acagcccgtc gagtttttcg cgctgctctg
    39601 gcatctgtaa atgctcgcga gctggcggat tttgtccatt attcaacagg taaggcttaa
    39661 ttttctcata ttgcgcatag aattgtccca tgtctaccac caaatcgcgg atcaccggta
    39721 aacctggcag cgggcgaatc acaatcttct tgcccggctg gttgagtgcc gaaatcgggg
    39781 taatacaggc cagaccattc ttgccgttca tgttcagacc gtcggaaccg cacacacctt
    39841 cacggcagga gcggcggaac gacaggctgg gatctttctc tttcagctgg ataagcgcat
    39901 ccagcagcat catgtcgcga ccttcttccg cttccagggt gtaatcctgc atacgcggag
    39961 catcatcaac atccgggtta tagcgataaa ttgaaaactc gagtctcatt ttcctgtctc
    40021 cgcattagta agtacgaatc ttcggcggga atgccgggcg cagtttcggt tccatgttga
    40081 cgcttcggcg cgtcatggat tccgactcag gcagatacag ggagtggcac agccagtttt
    40141 catcatcacg atccgggaag tcgaagcggc tatgtgcgcc acggctttcg gtacggaagt
    40201 tggcagaaac agccgttgca tacgccgttt ccatcaggtt atccagttcc aggcactcaa
    40261 cgcgctgggt gttgaactcg ctggaagtgt catccagacg ggcatttttc agacgctcgc
    40321 ggatcacttt caactgctca agccctttcg ccatcgcatc accttcacgg aagaccgaga
    40381 agttatgctg catacattct tgcagcgctt tacggatcgc caccggatct tcaccgttac
    40441 gattattgtt ccaccggttc aggcgatcca gagacgcttc aacatcagac tcgctggcat
    40501 cgtgcagtgc gccctgctcg gcgatagact cttgcagatg cagacctgcc gcgcgaccaa
    40561 agaccaccag gtccagcagc gagttgccgc ccagacggtt agcgccgtgt accgatacac
    40621 aagcgatttc accaacggca aacagtcccg gaacaaccac atcttcgcct ttctcattca
    40681 cagtcagcgc ctgaccggta actttggtcg gaataccgcc catcatgtag tgacaggttg
    40741 ggataaccgg aatcggctct ttcaccggat cgacgtgagc gaaggtacgg gaaagctcca
    40801 ggatacccgg cagacgggat tcgagaactt ctttacccag gtgatcgagt ttcagtttcg
    40861 cgtgtggccc ccacggacca tcacagccgc gaccttcacg gatttcaatc atgatggaac
    40921 gcgccaccac atcacggccc gccaggtctt tggcgttcgg cgcataacgc tccataaagc
    40981 gttcgccatg tttgttcagc agataaccgc cttcaccacg gcaaccttcg gtgaccagta
    41041 cgcccgcacc ggcaatgccg gtcgggtgga attgccacat ttccatatcc tgcaccggta
    41101 cgcctgcgcg gatagccatg ccgacaccgt cgccagtgtt aatgtgggcg ttagtggtgg
    41161 actgataaat acgccctgct ccgccagtcg ccagcacggt agcgcgggct ttgaaataaa
    41221 ccacttcacc ggtttcgatg cacagtgcgg tacaacccac caccgcgcca tcctggtttt
    41281 tcaccagatc cagcgcatac cactcggaga aaatggtggt gtggtttttc aggttctgct
    41341 gataaagcgt gtgcaacagt gcgtgaccgg tacggtcagc agccgccgca gtgcgtgccg
    41401 cctgctcgcc gccgaagttt ttcgactgac cgccaaacgg acgttgatag atacgaccat
    41461 catcgagacg cgagaacggc aggcccatgt gttcgagttc cagaatcgct tccggcccgg
    41521 ttttacacat atattcaatc gcgtcctggt caccgatata gtccgaccct tttacggtgt
    41581 cgtacatatg ccattcccag ttatcttcat gggtattacc cagcgcaacg gtaatgccgc
    41641 cttgcgcaga aacggtatgg gaacgggtcg ggaagacttt agagagcagg gcacaggtct
    41701 ggccgctctg ggaaatttgc agcgccgcgc gcatacctgc gccaccggca ccaatcacaa
    41761 ctgcatcaaa ttctctgact ggcaatttca tcacacaccc cacaccacaa cgaatccata
    41821 aatcacgtaa accaccagtg caacgacaat caccagttgc agcatcaggc gcaaggccag
    41881 cggtttaacg tagtcggtca acacctgcca catgccgatc caggcatgaa tcaagataga
    41941 aaacagcgcc agcagggtga acactttggt gaacgcagag gcgaagaaac cgatccagac
    42001 ttcatatgtc agttcgccac tggtggcgaa aaaaccgacc atataaatga tgtagagcgt
    42061 caggacgata gcggtagcgc gaacgaggat gaaatcatgt acgccattgc gtcctaatgc
    42121 ggaggcgttg cttaccatac gaggactcct gcgagaagtg aaagcacgac agtaataaca
    42181 aaggagattt tggcggagcg tttacccgct tcgaatgttt cttccagata gccaaaatcc
    42241 atcatcatgt ggcgaatacc tacgacgacg tgatacgcca gagcggtaag gatgccccac
    42301 atgataaatt tgacgaagaa gctgcccata atcgcggaag cttgctcgaa accttcaggg
    42361 gaagagaggc tggtacccag aagccactgc aggatgccca ctgcaacaaa ggtgatcaca
    42421 ccggaaacgc gatggagaat ggacgctatc gccgtgatgg ggaaccggat ggtctgtagg
    42481 tccagattaa caggtctttg ttttttcaca tttcttatca tgaataacgc ccacatgctg
    42541 ttcttattat tccctgggga ctacgggcac agaggttaac tttctgttac ctggagacgt
    42601 cgggatttcc ttcctccggt ctgcttgcgg gtcagacagc gtcctttcca taactgcgcg
    42661 tcatgcaaaa cactgcttcc agatgcgaaa acgacacgtt acaacgctgg gtggctcggg
    42721 attgcagggt gttccggaga cctggcggca gtataggctg ttcacaaaat cattacaatt
    42781 aacctacata tagtttgtcg ggttttatcc tgaacagtga tccaggtcac gataacaaca
    42841 tttatttaat ttttaatcat ctaatttgac aatcattcaa caaagttgtt acaaacatta
    42901 ccaggaaaag catataatgc gtaaaagtta tgaagtcggt atttcaccta agattaactt
    42961 atgtaacagt gtggaagtat tgaccaattc attcgggaca gttattagtg gtagacaagt
    43021 ttaataattc ggattgctaa gtacttgatt cgccatttat tcgtcatcaa tggattcttt
    43081 acctgcaagc gcccagagct ctgtacccag gttttcccct ctttcacaga gcggcgagcc
    43141 aaataacaaa cgggtaaagc caggttgatg tgcgaaggca aatttaagtt ccggcagtct
    43201 tacgcaataa ggcgctaagg agaccttaaa tggctgatac aaaagcaaaa ctcaacctca
    43261 acggggacac agctgttgaa ctggatgtgc tgaaaggcac gctgggtcaa gatgttattg
    43321 atatccgtac tctcggttca aaaggtgtgt tcacctttga cccaggcttc acttcaaccg
    43381 catcctgcga atctaaaatt acttttattg atggtgatga aggtattttg ctgcaccgcg
    43441 gtttcccgat cgatcagctg gcgaccgatt ctaactacct agaagtttgt tacatcctgc
    43501 tgaatggtga aaaaccgact caggaacagt atgacgaatt taaaactacg gtgacccgtc
    43561 ataccatgat ccacgagcag attacccgtc tgttccacgc tttccgtcgc gactcacatc
    43621 caatggcagt catgtgtggt attaccggcg cgctggcggc gttctatcac gactcgctgg
    43681 atgttaacaa tcctcgtcat cgtgaaattg ccgcgttccg cctgctgtcg aaaatgccga
    43741 ccatggccgc gatgtgttac aagtattcca ttggtcagcc atttgtttat ccgcgcaacg
    43801 atctctccta tgccggtaac ttcctgaata tgatgttctc cacgccgtgc gaaccgtatg
    43861 aagttaatcc gattctggaa cgtgctatgg accgtattct gatcctgcac gctgaccatg
    43921 aacagaacgc ctctacctcc accgtgcgta ccgctggctc ttcgggggcg aacccgtttg
    43981 cctgtatcgc agcaggtatt gcttcactgt ggggacctgc gcacggtggt gctaacgaag
    44041 cggcgctgaa aatgctggaa gaaattagct ccgttaaaca cattccggaa tttgttcgtc
    44101 gtgcgaaaga taaaaatgat tctttccgcc tgatgggctt cggtcaccgc gtgtacaaaa
    44161 attacgaccc gcgcgccacc gtaatgcgtg aaacctgcca tgaagttctg aaagagctgg
    44221 gcaccaaaga tgacctgctg gaagtggcta tggagctgga aaacatcgcg ctgaacgacc
    44281 cgtactttat cgagaagaaa ctgtacccga acgtcgattt ctactctggt atcatcctga
    44341 aagcgatggg tattccgtct tccatgttca ccgtcatttt cgcaatggca cgtaccgttg
    44401 gctggatcgc ccactggagc gaaatgcaca gttacggtat gaagattgcc cgtccgcgtc
    44461 agctgtatac aggatatgaa aaacgcgact ttaaaagcga tatcaagcgt taatggttga
    44521 ttgctaagtt gtaaatattt taacccgccg ttcaaatggc gggttaattt ttatatgcct
    44581 aaacacaaaa aattgtaa
//
LOCUS       DAZPAB010000012        44404 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZPAB010000012 DAZPAB010000000
VERSION     DAZPAB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.012"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            19..1785
                     /gene="adeD"
                     /locus_tag="ACHUPG_000596"
     CDS             19..1785
                     /gene="adeD"
                     /locus_tag="ACHUPG_000596"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR0860334.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(1831..3222)
                     /gene="uhpT"
                     /locus_tag="ACHUPG_000597"
     CDS             complement(1831..3222)
                     /gene="uhpT"
                     /locus_tag="ACHUPG_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR0860335.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3360..4679)
                     /gene="uhpC"
                     /locus_tag="ACHUPG_000598"
     CDS             complement(3360..4679)
                     /gene="uhpC"
                     /locus_tag="ACHUPG_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR0860336.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4689..6191)
                     /gene="uhpB"
                     /locus_tag="ACHUPG_000599"
     CDS             complement(4689..6191)
                     /gene="uhpB"
                     /locus_tag="ACHUPG_000599"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR0860337.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6191..6781)
                     /gene="uhpA"
                     /locus_tag="ACHUPG_000600"
     CDS             complement(6191..6781)
                     /gene="uhpA"
                     /locus_tag="ACHUPG_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR0860338.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6856..7146)
                     /gene="ilvN"
                     /locus_tag="ACHUPG_000601"
     CDS             complement(6856..7146)
                     /gene="ilvN"
                     /locus_tag="ACHUPG_000601"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR0860339.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7150..8838)
                     /gene="ilvB"
                     /locus_tag="ACHUPG_000602"
     CDS             complement(7150..8838)
                     /gene="ilvB"
                     /locus_tag="ACHUPG_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR0860340.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8944..9042)
                     /gene="ivbL"
                     /locus_tag="ACHUPG_000603"
     CDS             complement(8944..9042)
                     /gene="ivbL"
                     /locus_tag="ACHUPG_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR0860341.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9607..9696
                     /gene="tisB"
                     /locus_tag="ACHUPG_000604"
     CDS             9607..9696
                     /gene="tisB"
                     /locus_tag="ACHUPG_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR0860342.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9820..9894)
                     /gene="ysdE"
                     /locus_tag="ACHUPG_000605"
     CDS             complement(9820..9894)
                     /gene="ysdE"
                     /locus_tag="ACHUPG_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR0860343.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            9976..11160
                     /gene="emrD"
                     /locus_tag="ACHUPG_000606"
     CDS             9976..11160
                     /gene="emrD"
                     /locus_tag="ACHUPG_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR0860344.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11168..11665)
                     /locus_tag="ACHUPG_000607"
     CDS             complement(11168..11665)
                     /locus_tag="ACHUPG_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR0860345.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11662..12024)
                     /locus_tag="ACHUPG_000608"
     CDS             complement(11662..12024)
                     /locus_tag="ACHUPG_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR0860346.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12014..12361)
                     /locus_tag="ACHUPG_000609"
     CDS             complement(12014..12361)
                     /locus_tag="ACHUPG_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR0860347.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12470..12883
                     /gene="yidI"
                     /locus_tag="ACHUPG_000610"
     CDS             12470..12883
                     /gene="yidI"
                     /locus_tag="ACHUPG_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR0860348.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(12930..14372)
                     /locus_tag="ACHUPG_000611"
                     /pseudo
     CDS             complement(12930..14372)
                     /locus_tag="ACHUPG_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(14407..16122)
                     /locus_tag="ACHUPG_000612"
     CDS             complement(14407..16122)
                     /locus_tag="ACHUPG_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFR0860349.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            16289..17182
                     /locus_tag="ACHUPG_000613"
     CDS             16289..17182
                     /locus_tag="ACHUPG_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR0860350.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(17179..17993)
                     /locus_tag="ACHUPG_000614"
                     /pseudo
     CDS             complement(17179..17993)
                     /locus_tag="ACHUPG_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17993..19609)
                     /locus_tag="ACHUPG_000615"
     CDS             complement(17993..19609)
                     /locus_tag="ACHUPG_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR0860351.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            19904..20578
                     /locus_tag="ACHUPG_000616"
     CDS             19904..20578
                     /locus_tag="ACHUPG_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR0860352.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(20617..22278)
                     /locus_tag="ACHUPG_000617"
     CDS             complement(20617..22278)
                     /locus_tag="ACHUPG_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR0860353.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(22474..22902)
                     /gene="ibpB"
                     /locus_tag="ACHUPG_000618"
     CDS             complement(22474..22902)
                     /gene="ibpB"
                     /locus_tag="ACHUPG_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR0860354.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(23014..23427)
                     /gene="ibpA"
                     /locus_tag="ACHUPG_000619"
     CDS             complement(23014..23427)
                     /gene="ibpA"
                     /locus_tag="ACHUPG_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR0860355.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(23421..23493)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            23732..24064
                     /locus_tag="ACHUPG_000620"
     CDS             23732..24064
                     /locus_tag="ACHUPG_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR0860356.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(24066..25298)
                     /locus_tag="ACHUPG_000621"
     CDS             complement(24066..25298)
                     /locus_tag="ACHUPG_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR0860357.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTWTPDSQWLVFDVRPSGASFT
                     GETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHR
                     RGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPA
                     LDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVG
                     NHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQR
                     RLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTH
                     NKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDG
                     QWLAWMEGGQLWITETDR"
     gene            25399..26499
                     /gene="cbrA"
                     /locus_tag="ACHUPG_000622"
     CDS             25399..26499
                     /gene="cbrA"
                     /locus_tag="ACHUPG_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR0860358.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(26460..27797)
                     /locus_tag="ACHUPG_000623"
     CDS             complement(26460..27797)
                     /locus_tag="ACHUPG_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0860359.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(27872..29020)
                     /gene="dgoD"
                     /locus_tag="ACHUPG_000624"
                     /pseudo
     CDS             complement(27872..29020)
                     /gene="dgoD"
                     /locus_tag="ACHUPG_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(29017..29634)
                     /gene="dgoA"
                     /locus_tag="ACHUPG_000625"
     CDS             complement(29017..29634)
                     /gene="dgoA"
                     /locus_tag="ACHUPG_000625"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR0860360.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(29618..30496)
                     /gene="dgoK"
                     /locus_tag="ACHUPG_000626"
     CDS             complement(29618..30496)
                     /gene="dgoK"
                     /locus_tag="ACHUPG_000626"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR0860361.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(30493..31182)
                     /gene="dgoR"
                     /locus_tag="ACHUPG_000627"
     CDS             complement(30493..31182)
                     /gene="dgoR"
                     /locus_tag="ACHUPG_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR0860362.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            31460..32116
                     /gene="yidX"
                     /locus_tag="ACHUPG_000628"
     CDS             31460..32116
                     /gene="yidX"
                     /locus_tag="ACHUPG_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFR0860363.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(32162..32974)
                     /gene="yidA"
                     /locus_tag="ACHUPG_000629"
     CDS             complement(32162..32974)
                     /gene="yidA"
                     /locus_tag="ACHUPG_000629"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR0860364.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(33088..33486)
                     /locus_tag="ACHUPG_000630"
     CDS             complement(33088..33486)
                     /locus_tag="ACHUPG_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR0860365.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(33726..36140)
                     /gene="gyrB"
                     /locus_tag="ACHUPG_000631"
     CDS             complement(33726..36140)
                     /gene="gyrB"
                     /locus_tag="ACHUPG_000631"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR0860366.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(36169..37242)
                     /gene="recF"
                     /locus_tag="ACHUPG_000632"
     CDS             complement(36169..37242)
                     /gene="recF"
                     /locus_tag="ACHUPG_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR0860367.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(37242..38342)
                     /gene="dnaN"
                     /locus_tag="ACHUPG_000633"
     CDS             complement(37242..38342)
                     /gene="dnaN"
                     /locus_tag="ACHUPG_000633"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR0860368.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(38347..39750)
                     /gene="dnaA"
                     /locus_tag="ACHUPG_000634"
     CDS             complement(38347..39750)
                     /gene="dnaA"
                     /locus_tag="ACHUPG_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR0860369.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            40047..40127
                     /locus_tag="ACHUPG_000635"
     CDS             40047..40127
                     /locus_tag="ACHUPG_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0860370.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            40357..40497
                     /gene="rpmH"
                     /locus_tag="ACHUPG_000636"
     CDS             40357..40497
                     /gene="rpmH"
                     /locus_tag="ACHUPG_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR0860371.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            40514..40873
                     /gene="rnpA"
                     /locus_tag="ACHUPG_000637"
     CDS             40514..40873
                     /gene="rnpA"
                     /locus_tag="ACHUPG_000637"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR0860372.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            40837..41094
                     /gene="yidD"
                     /locus_tag="ACHUPG_000638"
     CDS             40837..41094
                     /gene="yidD"
                     /locus_tag="ACHUPG_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR0860373.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            41097..42743
                     /gene="yidC"
                     /locus_tag="ACHUPG_000639"
     CDS             41097..42743
                     /gene="yidC"
                     /locus_tag="ACHUPG_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR0860374.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            42849..44213
                     /gene="mnmE"
                     /locus_tag="ACHUPG_000640"
     CDS             42849..44213
                     /gene="mnmE"
                     /locus_tag="ACHUPG_000640"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR0860375.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        10772 a        12037 c        11352 g        10243 t
ORIGIN      
        1 aatcaaaatg agaaaaatat gaataattcc attaaccata aatttcatca cattagccgg
       61 gctgaatacc aggaattgtt agccgtttcc cgtggcgacg ctgttgccga ttatattatt
      121 gataatgtct ctattctcga cctgatcaat gccggagaaa tttccggccc aattgtgatt
      181 aaaggacgtt acattgccgg tgttggcgca gaatacgctg atactccggc tttgcagcgg
      241 attgatgctc gcggcgcaac ggcggtgcca gggtttattg atgctcacct gcatattgaa
      301 tccagcatga tgacgccggt cacttttgaa accgctaccc tgccgcgcgg cctgacgacc
      361 gttatttgcg accctcatga aatcgtcaac gtgatgggag aagccggatt cgcctggttt
      421 gcccgctgtg ccgaacaggc aaggcaaaac cagtacttac aggtcagctc ttgcgtgccc
      481 gctctggaag gctgcgatgt taacggtgcc agttttaccc ttgaacagat gctcgcctgg
      541 cgggaccatc cgcaggttac cggccttgca gaaatgatgg actaccctgg cgtaattaac
      601 gggcagaatg cgctgctcga taaactcgat gcatttcgct acctgacgct ggacggtcac
      661 tgtccgggtt tgggtggcaa agaacttaac gcctatattg ctgcgggtat tgaaaactgc
      721 cacgaaagtt atcagctgga agaaggacgc cggaaattac aactcggcat gtcgttgatg
      781 atccgcgaag ggtccgctgc cagcaatctc aacgcactgg caccgttgat caacgaattt
      841 aacagcccgc aatgcatgct ctgtaccgat gaccgtaacc cgtgggagat cgcccatgaa
      901 gggcatatcg atgccttaat tcgccgcctg atcgaacaac acaatgtgcc gctgcatgtg
      961 gcatatcgcg tcgccagctg gtcgacggcg cgccactttg gtctgaatca cctcggctta
     1021 ctggcacccg gcaagcaggc cgatatcgtc ctgttgagcg atgcgcgtaa ggtcacggtg
     1081 cagcaggtac tggtgaaagg cgaaccgatt gatgcgcaaa ccttacaggc ggaagagtcg
     1141 gcgagactgg cgctgtctgc cccgccatat ggcaatacca ttgcccgcca gccagtttcc
     1201 gccagcgact ttgccctgca attcacgccc ggaaaacgct atcgggtcat tgacgtcatc
     1261 cataacgaat tgattacgca ctcccgctcc agcgtctaca gcgaaaatgg ttttgatcgc
     1321 gatgatgtgt gctttattgc cgtacttgag cgttacgggc aacggctggc tccggcctgt
     1381 ggtttgctcg gcggctttgg cctgaatgaa ggcgcgctgg ctgcgacagt cagccatgac
     1441 agccataata ttgtggtgat cggtcgcagt gccgaagaga tggcgctggc ggtcaatcag
     1501 gtgattcagg atggcggcgg gctgtgcgtg gtacgtaacg gccaagtaca aagtcatctg
     1561 ccgttaccca ttgccgggct gatgagcacc gacacggcgc agtcgctggc ggaacaaatt
     1621 gacgccttga aagccgccgc ccgtgaatgc ggtccgttac ccgatgagcc gtttattcag
     1681 atggcgtttc tttctctgcc agtgatcccc gcgctaaaac taaccagtca ggggctattt
     1741 gatggcgaga agtttgcctt cactacgctg gaagtcacgg aataataaaa aaagcccggc
     1801 gtcatgccgg gcaaaagtca ccagttacgt ttatgccact gtcaactgct gaattttttt
     1861 ctcgcggcgg attttgcgtt cttccattac cgccactatc gccatcaggc agatacaacc
     1921 aatcgcggcg atatccagcg cggcgaaggt gcctgcccag ccggtaaggc cgaataccgg
     1981 cgtcccatcg gcaatcattc ccagacctaa cttggcaaag ctgtcaccaa tcaggtaagc
     2041 aaaggtgcct ttaataccat cggcagcgcc aatcgctttt ttaggtacaa agccaacagc
     2101 agccacacca atcaacaatt gcgggccaaa gaccaggaaa cccaacgcaa agagagaagc
     2161 cagatagata tattcgttac tggcatgttg atacacaccg agcgtggcga taatcagcgc
     2221 cagcgcgatg caggccacca ggccacggcg accgttcgcc aggtcagaga gccagcccca
     2281 cagcagcgta ccgaccagcg caccagcttc aaacagcgta aagccctgaa tcgccaccgc
     2341 tttagagagt ttcagttcct ggaacgcgta tacggttgac cactggtcga taccaatacg
     2401 taccacatag aggaaaatgt tggcgaagca cagcagccag atcactttgt ttttcagcac
     2461 atactcaaca aagatctgcc acttggtcat atcggtagat tctgtctctt tgtcctcttc
     2521 gctgatctcc tcgccgaaca gttcttcagc tttgccgagg ccataagatt ccggggagtc
     2581 gctgccgtaa cgcaggccga taaaaccgac aatcagcgcg ataatcgacg ggaagataaa
     2641 catgccgatg acatggccat cgaacaggta atttgccccg aacagcgcca cacctgctgc
     2701 gcctgcaccg ccaaggttgt gagaaatatt ccagaaaccg aggaatgtcc cgcgtttacg
     2761 acgcggcgtc catttggtga tggtggagta actgcacgaa ccgccggtac tctggaaaaa
     2821 gccgcttaag gcgtagaagg caatcatcag gaacaggcta accgagccgc tgcccatact
     2881 ggcactgaag cccagcatac aaatggcaga gaggataagc atgaacggca ggaattgttt
     2941 ggtgtttttg ccgtcggcgt agtatgaaac caacgtttta cccacgccat aagtgatgga
     3001 gaaacccagg ccgatcatcc ccagctgcgt catgctcaac ccgtaggtcg aaatcatatc
     3061 gttctgcgcg atgttaaagt tcttgcgaat caggtacatc gtcaggtagc cgataaagac
     3121 caccaggtag gattgcatga acggtttgaa ccacattttg cgccgcactt cgagcggaag
     3181 gtccagggtc ggcttgcgaa cttggtttag gaaagccagc atgggttact cctgaaatga
     3241 atacctgcgt gaggcatgca ttgtaaaaat cagcagcaaa tcaggcctga gatagcgtcc
     3301 aagaaaaacc tggaaaattt cttagttttg ccggatatga agtgaaaagg tgagatgcat
     3361 cacgcttcac gcggtgtctg ggcgttcaaa aagggcagta acagcagtgc ggaaatcccg
     3421 gcggcgatag agataaccac aaaaaatccg ctccagtgcc aggtatcgag cactttcgcc
     3481 agcggccaac cagcaagcga cgcccccaga taagcaaaca agccgacaaa ccccgtcgcc
     3541 gcccctgccg cctctttgtg ggaacactct gccgccgcca taccaattaa catctgcggg
     3601 ccaaagacaa aaaaaccaat ggtgaagaag caggttgcct gcatcacgta gctggcaaat
     3661 ggcatcagcc acagcgagcc gactgaaagc aaaattccgg cggcgaaaat caaattcatc
     3721 ggcccacggt tgccgttaaa caatttgtcc gagccccaac cggctaccag cgcaccgata
     3781 aatccgccca gttcaaacat cgtcactgcc gtattcgccg tgaccagatc gacgcccagc
     3841 gtctcggaca tatacaaatt gccccagtcg ttgatcgccg cccggaccac atagaccagc
     3901 acatagcaaa acgaaagcag ccagatatac ggattcagca acacatattt ggtgaggatc
     3961 tctttacgcg tcaaccctgc cccttcttgt tgttgagcaa tttccagcgc gtcgtgccgc
     4021 cattcaccga ccgccggtaa acctaacgcc tgcgggcgat cgcgtagccg ccagcagaga
     4081 aaaatcccca cgactatcgc catacaacca gcaatcatca tcccggcacg ccagccgtaa
     4141 tgcagcgcgc tcgctgccat cacaatggga atgagtgcgc cgccgacgtt atgcgccgtg
     4201 ttccataatg cccaccaacc gccgcgctcg gtacgtgaat accaggccgt taacagacgc
     4261 gcacacaccg gtgaacccca gccctggaaa aaggcgttca gcacccagag cacggcaaac
     4321 gcccatagcg acgtcgagaa gccaaacaga atgttcatga tgcccgtggc gataagccct
     4381 atccccataa aataacgggc atttgagcga tcgctgacaa tgccggagac aaacttcgac
     4441 acgccgtaag taatgtaaaa cagggtcgct aacaggccga tatcgctacg gctgagcacg
     4501 ccgttagcaa ggatttctgg taccgcggcg ttaaaacttt tccgcgtgaa gtaaaacagc
     4561 gcgtaaccca gccagatggt cagcagaata tgccgacgcc agtagcgata acgggcatca
     4621 atttcatgtt tatcagtcat taatggcgca tcggcaggcg ctttcagaaa cggcaacatc
     4681 atcgaacctt agacataacg ttgaggtaga gaaacgctga cacgcgtgcc gtgcagacag
     4741 gaaatgtgta atgtgccgcc cagcgccgtt acgcgctcgc gcattccggt gaggccaaaa
     4801 ccttgttgcc cggaacccgg cggcaaaccg ctgccatcgt cttcaatcac cagcatcaac
     4861 cgttcatcct gctgccagcc ttgcagcgta accgcgctgg catcggcatg tttcacaatg
     4921 ttgttcagcc cttcctggca gacacgaaac agcgtcacgc gctggttttc gcttaatgct
     4981 gattcatcga tacgccattc gagatggctg acaatgccgc gcccttccag ctccatttcc
     5041 cgcatcagtg agcggatggc ctgctccagg gtgagatcat ccagctggcg cggacgtaac
     5101 cgtcctaaca aacggcgcac cgcgtcgtaa acgcccagcg acagttgttc gatgagctgc
     5161 ccgctctgct tcacactggc gttatctgcc gccagccgct gaacaatgcc cgcctgagta
     5221 cgaatagcag tgatggtctg accgatatcg tcgtgcagct cacgcgccac gtcgcggcgc
     5281 acgctctctt cggtttctaa caaacgttca gccagatgct gattgcgcgc cagttccttt
     5341 tgtagcgact ggttaagttc acgcaaccgc tggatgcctg cgcccagtaa caaccctgtc
     5401 agactttgca ccagcaacga gagtaataaa tccaccagat gatcgcgcca ggtttgactg
     5461 gcgatcagcg cgatggcgtt catcaacgtc gcaatcagcg ctccttgcca gccatagtgc
     5521 caggccagcg cgataatcgg cagcgccaga cagaatggcg taaagcgcga cagttcgtcc
     5581 ggcaatccca actggagcca gagactgata acaaacagca gcaagtacca gaccagatgc
     5641 cgcccgcgcc agttgatggg ctgagaaacc agtgacgggc cgagcggcag ccaggtgtta
     5701 ttggcgagat agtgccagaa caccagacat atcggggcca gcgtcagacc gccagttaaa
     5761 gtcagcaaca gcgcattcca cgactctttg ccgtgccaaa gccagggcag cgactgcaac
     5821 aacgccgccg ccgttaacgc cgcaccctgt aacagcaagg tgcgccagtc acgctgatgg
     5881 cgatagcgcg agatcagcgc tacgggcagt aacgtcagca aactaccgat catcaataac
     5941 ggaaaatggg ttaaaccgac cgcctgcgtt agccagtaaa tcagcagcca ctccgcaccc
     6001 agcaatacgg gccagtatcc gcgcgggcat tgcagcatta gccccagacg cagaccaaac
     6061 ggaaataaca gcaccgccat atcagggcgc tcaaccagat gcaggctgat actccatagg
     6121 caaaaccatg cggcagagaa gataaaaaag caggcaataa cggtaattaa gcgggagaac
     6181 aacgtcttca tcaccagcca tcaaacatgc ggcgcgccag ctctacgtcg ttactgacgc
     6241 ccagtttttc catcagattg gcgcgatgga cgtgtaccgt tttcggtgac aagcccagtt
     6301 cggcggcaat ctctttcacc gccattcctt gcgccagttt ttccgccacc tggcgttcac
     6361 gtttggtcag cgggtcctga cgaccggatg ccagtttaat ggcaatatcc ggcgtcagat
     6421 aacagccgcc cgtggcaacc gtatgcaccg cagcaatgag ttcatccggg ctacagcgtt
     6481 tggaaagaaa gccgcgtgcc cccgcgttaa gcgcctgctc aaccagcgca ggactgtcgt
     6541 gaacggagag cataatcgtc gccatacctt tcggcagctg gcttagcagc tccagaccgg
     6601 agatatcggg catggagata tcgcaaatac acacctgcac accgcgcccc ggcagccccg
     6661 ccagcgcctc gcgccccgaa ccaaactcgg caactacctg caaatcaggt tccagcccca
     6721 gcagctgcgc aaagccggag cggacgatga ggtgatcgtc tataagggca acggtgatca
     6781 tggtcttgtc ctggcgggta aaaaaaaacg cgcttacctt aacgataagc gcgatgttgt
     6841 tcaagccttg agcggttact gaaaaaacac cgcgatcttg ttaaacatcg tcggatcgga
     6901 ctgattacgc tgcactttca cgacatcttc cagcttagcg atttggctta tcatctgctc
     6961 cagacgctgg tcgtcattga ccagtagcca gatatggctt ttgtcgctgt cctgaatcgg
     7021 cagacaaaga atgccttcaa cgttaaaagc gcggcgggca aaaaggccac aaacgtgggt
     7081 cattacgccc ggatggttgc gaacggtgag ctccagaatt acgttgttat gagttgtgtt
     7141 ttgcatggct tattccccca ccatttcagt attcgccgca cctggcggca ccatcgggta
     7201 aactttttct tcggcatcaa tgcgcacatg gatcagcgcc gggccagggc gattgatgat
     7261 ttcctgcaat gcagcctgcg gatcggcttc gttattcaaa tcacaggttt cgaggccgaa
     7321 tccggcggca atctgcataa agttgatttt gcccggatag gtggcggcaa aaacgccttg
     7381 ctcgtagaac agactctgtt gctgatgcac cagccccagc gcttcgttgt tcatcagaat
     7441 gattttgaca tccagctgat tttcactggc ggtcgccatc tcctgaatat tcatcatcag
     7501 gctgccgtcg ccggagaaac acaacacttt gcgatccggg ttcgccagcg ccgcgccaat
     7561 cgccgcaggc aggccaaaac ccatcgtgcc cagcccaccg gaggtcagcc actggcgtgg
     7621 gcgattgagc ggataagctt gcgcggtcca catctgatgc tgacccacat cggtggtgat
     7681 aatcgcattg tcatcgacac aggcggcaac ggcgttgatc aggccgtaat ggcttaatgg
     7741 atcgcacgct ttcgggattg gacacgggaa ttcgcgctgc aaatccgcta ccaactggtg
     7801 ccactctgca cgcggttgcg cttccacccg cgggatcaat tgcgccagca cgtcatcaac
     7861 atccgcctga atcgccacat gcggctgctt gattttaccc agctctgcgc gatcgatatc
     7921 gacatgaata attttggcat tcggacagaa ctgctcggtt ttgccaatcg cccggtcatc
     7981 aaaacgcgca ccgagcacaa tcaacaaatc cgcctcctgc aaaatatagt tggtactgcg
     8041 cacgccgtgc atccccagca tacccagcga caacggatgc gcttttggca gcatgcccag
     8101 cgccattaaa gtcatggtgg taggcagttg cgctttctcc gccagttcac gcacccgcgc
     8161 aggcgcattg atcacaccac cacccagata aagcaccggg cgtttggcag cgttaatcat
     8221 tgtagctgcg tcacgaatgc tttcttcgct aaaggcgggt gcaacggctt tttctgccac
     8281 cgcgggctga gcttcaatct caaaaaccgc cgtttgtaca tccttaggaa tgtctatcca
     8341 caccgggcta gggcggcctg attgcgcaat gcggaaggca tcgctcatga cctgtgggag
     8401 ctcttcgata tgtctgacca gatagttgtg tttggtgatg gggatagaga tgccgtaggt
     8461 gtccacttct tggaaggcgt cggtgccgat catcgaggcg ggaacctgac cagtgatgca
     8521 aatcagcggg atggagtcca gccgcgcatc agcaatggcg gtcaccaggt tagtcgcacc
     8581 cggcccgcta caggccatac agaccgccgg tttaccgtcg gtgcgcgcca ttccctgagc
     8641 gataaacccc gcgccctgtt catggcgagc cagaatatgg cggatttgcg tgctttggct
     8701 taaggcgtcg taaacaggca ggatagaacc gcccggaatg cccgtcacaa tcttaatgcc
     8761 ctgctgttcc aggaaatgaa cgataaattc tgcgccggta aagcgcttac gcgtcgatgt
     8821 tgtgcccgaa cttgccatgc tccagtcctt ttcttctggg ccgactttcc gggaggtgct
     8881 taaacgaaaa accccgcccg gtttgcgccg gcggggtttt ggaatcgtgt gttgttccag
     8941 tccctacggc gcattgccga cgaccaccac cacacgcacg acgaccactg cggcggatgg
     9001 cgcagttggt agtagttttg cgttgaacat ggaagtagtc attggggacc ttgttggttt
     9061 tgtgtttaac aatatttata caagcacagc tttacagggg agacaatgga aaatttttca
     9121 gcaagggaaa attgaggggt tgatcacgtt ttgtactgaa ttgcagataa caaaaaaccc
     9181 cgccggagcg aggtttcgtc agccgcctgc ggctggtaac cgcaaagcac actgtattat
     9241 gtcaacactg aaagtatacg tgttccgcgc agaacgcgca atttcggcac gaattttgac
     9301 gtatttagtg catagttgag tatcgttcac agtttgcgtt ttgtccaaat attactgttt
     9361 atttatacag taagcttcta taatatcact gtacgcaatg tgttatgcgg gggccgcatc
     9421 gttacccggc gcactaagtc ctggctgaaa cgggtggtgc cgtcagcgcc ttaaccccgc
     9481 gtgagcacac tgtgttatgt caacaagcac aacgtttctc cttgagatac cgcgtgcaca
     9541 acagctggca acaggcagcg gaaaggtacg tcagctggca gtgctcctga accacaggag
     9601 acgcgtatga gcctggtgga tatcgccatt cttatcctca aactcattgt tgcagcactg
     9661 caactgcttg atgctgttct gaaatacctg aagtaattca gattcaagtc gcaccaaagg
     9721 ggagcgggta accgctcccc ttttatattt agcgtgcggg ttggagtcgg atgcgatgct
     9781 gacgcatctt atccgcccta ccatccctcc cggcaacatt tattgccgct tttgtttaca
     9841 tattctgccg ctaaacaatt ccccattcct ggcgtatatc tggctaacat tcatcaatgt
     9901 gatagattcc tctcccgcat ttatgggaat gcgtagtgac ttattctaat tatttttata
     9961 aaagcatccg tgataatgaa aaggcaaaga aacgtcaatt tgttattgat gttggtatta
    10021 ctcgtggccg tcggtcagat ggcgcaaacc atttatattc cagctattgc cgatatggcg
    10081 cgcgatctca acgtccgtga aggggcggtg cagagcgtaa tgggcgctta tctgctgact
    10141 tacggtgtct cacagctgtt ttatggcccg atatccgacc gtgtcggtcg ccgaccagtg
    10201 atcctcgtcg gaatgtccat ttttatgctg gcaacgctgg tcgcggtcac gacctccagt
    10261 ttgacggtat tgattgccgc cagcgcgatg caggggatgg gcaccggcgt tggcggcgta
    10321 atggcgcgta ctttgccgcg tgatttatat gaacggacac agttgcgcca tgctaacagc
    10381 ctgttaaaca tggggattct cgtcagtccg ttgctcgcac cgctaatcgg cggtctgctg
    10441 gatacgatgt ggaactggcg cgcctgttat ctctttttgt tggttctttg tgctggtgtg
    10501 accttcagta tggcccgctg gatgccggaa acgcgtccgg tcgatgcacc gcgcacgcgc
    10561 ctgcttacca gttataaaac gcttttcggt aacagcggtt ttaactgtta tttgctgatg
    10621 ctgattggcg gtctggccgg gattgctgcc tttgaagcct gctccggcgt gctgatgggc
    10681 gcggtgttag ggctgagcag tatgacggtc agtattttgt ttattctgcc gattccggcg
    10741 gcattttttg gggcatggtt tgccgggcgt cctaataaac gcttctcaac gttgatgtgg
    10801 cagtcggtta tctgctgcct gctggctggc ttgctgatgt ggatccccga ctggtttggc
    10861 gtgatgaatg tctggacgct gctcgttccc gccgcgctgt tctttttcgg tgccgggatg
    10921 ctgtttccgc tggcgaccag cggcgcgatg gagccgttcc ccttcctggc gggcacggct
    10981 ggtgcgctgg tcggcggtct gcaaaacatt ggttccggcg tgctggcgtc gctctctgcg
    11041 atgttgccgc aaaccggtca gggcagcctg gggttgttga tgaccttaat gggattgttg
    11101 atcgtgctgt gctggctgcc gctggcgacg cggatgtcgc atcaggggca gcccgtttaa
    11161 gcgcacgtca ccgcagcatc gtcatcagct ccatgggaga acgatgctgc tttatcagat
    11221 cacgcatcac ccgcatatgc ggtgcggagt aagaataaaa cgcctgatag cccgcacaaa
    11281 gcacgctgtc tggcgtctcc tgccgagcgt gcgggcacag gcgatgccag tcgcacgcct
    11341 ggcagtcacg gcgcggttgc gggttttcgc tgccacacca tttttgtaag gtggcgtcaa
    11401 aaagctcaat cgagatacga ctgatatctg cccgcaccca acactcaaac agggcaatta
    11461 aaaatttccc ccacagctct gctgtcaccg aagcggccgt cagatcgccc cggtcatcaa
    11521 attcaagata gggaatgagc tgtacggagc ggatgccccg ctgtttgaaa ttgtgataaa
    11581 aatcggcagg ttgcagagca tccttgtact ctacaaccag cttatgcctg tcctcttcag
    11641 catcaatgac ctgacttcct gtcatgcgac tctctcaata aatacgataa gttgatgtat
    11701 atgcgttaca gcaaacagta ttgcgagaga taacaccgcg agggagatca aaaatttgtc
    11761 acggactacg tgaaattggg aaaaatcgct attcgtgaca tccattaaat tgcgattacg
    11821 ggtgtagtgc cagaggatca gcgccacgat ggcgaggatg ccaatcgaaa tccagaataa
    11881 catgcccgcc tggtgccagt tgtgtttgat agccaacgcc atcaacgcgc cgtagcccag
    11941 catggtacga aaccacgcca gtgatgtgcg ctccggctgc agccctgggt cggcaatgcg
    12001 tctggcttta cggctatccg gcatacaaca ccagtcccat aacgatcacc gccacaacca
    12061 tcaatattaa gctgatgatt aataagctgt tggtgtaggg caggtcctct ttcagacgca
    12121 tcgctttttc attgcgtaac cagcgcaggt agccatacat tgctaacccg ccggaaaaca
    12181 gacacaacaa caacgccagc agttcacgaa tgacgggcgt ggcgaaatct ggcgcaagtt
    12241 gatcaagccc aacccccgcc gccagaaatc ctagtgcggt acggatccac gccagaaaag
    12301 tacgctcatt agccagcgag aagcggtaat cgggcgcttc tccaaggcgg gaaattttca
    12361 ttgttgctcc tttgcaattt cactaacgct ctatctttag cggttttttt taagaatttc
    12421 gtccgcccgt ttcctgatat gcttttgcca tccgctacaa ggaggtcaga tgggtatcat
    12481 agcgcaaaat aaaatcagtt cattgggcat gctgttcggt gccatcgctt taatgatggg
    12541 gattatccac ttttcattcg gccctttttc cgcaccaccg ccaacgcttg aaagcatcgt
    12601 ggcggacaaa acggctgaaa ttaaaagagg tcttctcgcc ggaataaaag gcgaaaaaat
    12661 taccactgtg gagaaaaaag aggacatgga tatcgataaa atcctcgatc aaagcggtat
    12721 cgcactggcc attgctgcgc tactttgcgc atttattggc ggtatgcgta aggaaaatcg
    12781 ctggggcatt cgcggcgcgc tggtttttgg tatcggcatc gtgtgcagca ttttgctgat
    12841 attcctgatt ttttccttcc tgaccggcgg ttcgctggtt taagcagcct gaataatgcc
    12901 cgccgggtga tcatcagcgg gcattatcgt cagaatttct gttttttctc ctccaccttc
    12961 actccttgcg tcggtaggcc ggtgtcatag tcgcgtacca ctggcgaata gccatcttgt
    13021 gggcgtgggc gaaacgcccc catccagcgc ggtcgtgcat ctttacgcca cggacgcaga
    13081 ctccattggt aactgcggaa cggatcgcga attttgtcca tgtaatccaa taaggcgtca
    13141 tgcattttgc tgcgaacgtc tgcaaaacgg atatcatcga tcaggttatg catttcattt
    13201 gggtcattac ggcgatcgta aagttcatca ctggtgaaga ggttgagcac cagtttaaag
    13261 tcatccgtca cccagcaacg caccggaata aaaccgccaa agctgtcatg ctcaatctcg
    13321 tagcggttaa attccaccat cacgccgcgt ggctctttca cggcaaggat attttccccc
    13381 ggcagaatct ctggtttttc aatatctgcc agcgccatca ttgtcggcag taaatcaata
    13441 tgactgactg gcgtatcgac ctgtcgccgc tccccttgcg gcgaacggat gatcagcgga
    13501 atacgggtga tgtcgtcata catcgccgct cctttactga tcagcttatg tgcgcccatc
    13561 atttcaccgt gatcggaggt ataaatgacc cacgtatttt cacgttgctc tggcgttaag
    13621 gcattgatga cacgtccgat ttggtcatca acaaagtcat tgcaggcaaa atagagcgga
    13681 tggtgataaa gcccgtcatc accgactggc gatggcatcg cctgcgccca taagcggtga
    13741 tgttccggtt tgttcgccag gtcatcctgt gatttctcgc caagttcgta gtaaaaatca
    13801 gcgtatttct ctaaatactc caccggacag gtgaacgggt gatgcggctc atcatacgaa
    13861 accaccatca ggaagggttc ctcggcgcgc acgggctatt gcagaaaatc taccgctcga
    13921 ttgctgatgc ggtgcgccca ggtgaaggtt tcgtcaatat ggttcgcctg taaatcttcg
    13981 acgctgttta ggccattgcg ccacaggctg atctcttttt ccgtcagttc gctaagatag
    14041 ttcgccccat caaaccagta atcagcatcc cactccggcg gacactcgcc agtgccgaaa
    14101 tagtcatgac cgtcgagatg ccatttgccg atgtaacagg tgtgatagcc ggcatcctta
    14161 aagtagcgcc ccatagtgga gatgtttttg cctggcgcga cgttgttggt ccacgggccg
    14221 gactggttag cgtagatacc ggtgaatagt ccggcccgtg caggcgtaca aaccggtgaa
    14281 caagtgtagg cggaattaaa gcgaatacct tccgccgcca gactatcaat attttgcgta
    14341 ttcagcggtt taccgctata gcaaccgacc atattggtgg cctgggtatc ggtcaattgg
    14401 ggcgtttcat aggctatcct tcgcgtcggg agtaaaggtt acggctgggt cgtgacgatg
    14461 ccaccagctg tcaaaaatca ggtagacaat cactacggca gcgatgaaat aactgatcat
    14521 cgctaaccag cgcgtaccgt agccgccgaa ttcagccagc ccgacataga cgccaatcat
    14581 cgcgaacagg ataccaattg acgcgatctt gacgtttttc cacggtttca tgtccaccgc
    14641 aaacgcatct ttgaaggtga acggcgtggc gcgcggtttg ataaaaccga tcaccagcat
    14701 cacgaccacg ttgatgcaga acgtacaggc cagcacatag aggaaatgga agtcgaactt
    14761 caccagatag ttgatggtga tgtagctgat tatgccaatc cccatcgcca cttttgccgc
    14821 cagcgccggg atgcgcggaa agaaaaagcc cataatgatg atggtaacca gcggcacgtt
    14881 gtaaatgccg ttgagctgtt tcatccagct atacagcccc tgcggcgcgt tcgcgatcca
    14941 cggcgctacc aaaaccgaaa cgatagcgat aaagaaacca aatttgcgcc cgacggtgac
    15001 cagctgctgc ggctcggcat tctggttaat gatgcgacgg taaataccca tactgaataa
    15061 tgtactggcg ctattcagaa agccgttgaa ggtactgatc accgcaccaa ataacaccgc
    15121 gccgaagaaa ccctccagtg gcactggcag aacgttattg accagcgtcg ggtaggccat
    15181 gtcggctttc ggtagatcct gatacagatg aaacgcaatc aaccctggca gcaccagtac
    15241 cagcgggtcg agcattttca gcaccgccgt taacagcgcc cccttttgcc cttccgccag
    15301 gctttttgac gccagcgtgc gttgcacgat gccctgattt gtacaccagt aaaaggtgtt
    15361 caccagaatt aaaccggtaa atgccgcgcc aatcggcaag ggatcggtcg ggccaccgac
    15421 tgagtttaat ttctcggcgt gaacggtggt aatttgctca atgccctgca taaagctgcc
    15481 cttgcctatc gcgatcaggc caaataccgg caccatcaac ccgccgataa ccagcccaat
    15541 accgttgatg gagtccgcca ctgccattgc gcgcagtccg ccgatcaccg catacaaaat
    15601 tcccgccaga ccaagcaaaa ttaccagtag ccagatagcc gcaccgtgag aaatctgtag
    15661 cgattccccg acgtgaaaca ggctgttgag cgccaacgcg ccggagtaga gaacaatcgg
    15721 cagaaagcag acgccggtgg caatgaggaa gcagaagtcg atgataatac gcgtcgtttt
    15781 atcataacgt tcctccagaa aatcggggat ggtggcaatg ccgcgcttca gatagcgcgg
    15841 taaaaagatt agcgcgagga agatcagcgt caccgctgaa gtcacttccc agcccatcac
    15901 cgacatgccg cttttgtagg cctgcccgga aagaccaacc agttgttccg tggaaaggtt
    15961 ggttaacatt aacgaagcgg caataaccgg cgcttttagt gaacgtccgg caagaaaata
    16021 gccttgttgc gatccggtat ctgttttgcg aaccttccac caggtgatca ccgccaccag
    16081 cagcgtaaaa ccgacaaaac tcaagatttg taacgaattc atctcatagc ccttatttat
    16141 gtttatgtcc cggacgcatg aatataggga gaggtaaaat gcgtcgggtc aggcagtcag
    16201 attcttaaaa tacaaacgtc gtatccctga acggattgtt ttctgttaag ttcaggttgt
    16261 gagcataatt cgccggaggg atattaaaat gaatggaaaa ttgcaaagtt cagatgtaaa
    16321 aaacgaaact ccgtacaata ttcccttatt gattaacgaa aatgtgatct ccagcggaat
    16381 ttctctgatc tcgctctggc atacctacgc cgacgagcat taccgggtga tctggccgcg
    16441 ggacaagaag aaaccgctta tcgccaactc atgggttgcg gtgtataccg tacaaggatg
    16501 tgggaaaatt cttttaaaga atggcgaaca aataactctg catagcaact gtattatatt
    16561 tttaaagcca atggatattc actcttatca ctgtgaaggt ttagtctggg aacagtactg
    16621 gatggaattt acccccacca gtatgatgga tatttccgtt ggtcagcaaa gcgttattta
    16681 taatggcgaa atttataatc aggaactcac cgaagttgct gagttaataa cttcaccaga
    16741 agcaataaaa aataatctgg cagtcgcttt tctgacgaaa attatttatc agtggatttg
    16801 tcttatgtcc gcagatggta aaaaagatcc acaacggcgg caaattgaaa aattaattgc
    16861 cactttacat gccagtctgc aacaacgctg gagcgtagct gatatggctg ccacgatccc
    16921 ctgtagcgaa gcctggttgc gtcgtctgtt tttacgctat accggcaaga cgccgaaaga
    16981 atattacctc gatgcgcgtc tggatctggc gctatcgcta ttaaagcaac aaggaaactc
    17041 ggttggcgaa gtcgctgata cgctcaactt cttcgactcc tttcatttca gcaaagcctt
    17101 taaacataaa tttggttatg cgccgtcagc cgtgctaaag aatacggacc agcacccaac
    17161 ggatgccagt ccacacaatt aacgtaactc tggccagtac gctttgttgg cctccagcaa
    17221 ctcatccaga atagctttgg cgaccgaggc gctcggtacc gttttcgaca acgtaatcgc
    17281 ctgccacagg tgctgatatg aacgctgttc ccaggcgtcc acgaccagtt tttccaccgc
    17341 cacttgctga ctcattaacc ctttctgaaa ctgcgggata tcgccgacca ccagtggttc
    17401 aggccgtagt agcgcacgcg catctgtttg cgatcttgca gcccaacaat ttgcgccatc
    17461 cggctttcaa taccgattgg catgtcacag atgttgagga ttttcgcatt cgggcgcaga
    17521 cgacgcgtgg cttctgcgac aattgctgcc gggttggagt agttgagcat ctaggcgttt
    17581 ggtgaatatt tttccatata atccaccagt tccaggacgc cgccaatgga acgcatgccg
    17641 tacgctattc cgccgggtcc gcaagtttcc tgaccaacaa caccgtggcg cagtgggatt
    17701 ttttcatcca gttcgcgcat cgggtacttg ccgacgcgga tgtgcgccat gacaaaatca
    17761 acgtcgctga acgccacttc aggatcggtg gtgtaactaa acgcaatgtc cggcgctttt
    17821 tctttaagga tgactttaca ggcttcggca atcacttcct gccgcgcgcc atcgttatca
    17881 taaaatttca gtgcacgaag cgggaaacgg tcctgattcg ccaggagcat caacacgatg
    17941 cctggcgtaa aggtgcttcc accgcctgcg acaaccactg agaatttggt cattatactg
    18001 cctccgtaat ggcaacattt tctgctgatt gatgagaatt aattaagcta tcgagctgtt
    18061 cacgcaattg ggatacatgc agaccaatga tcacctgaat ggcatcgcca ctacggaaga
    18121 cgccgtgcgc tcccagcttt ttaaagactt cgtcatccag cgtttgtgac atgtcatgca
    18181 gtgcaatacg tagacgcgtc gcgcaattgt taatgctgga gatattgccg accccgccca
    18241 gggcttgcag gataccggca gcctgatcca gctctttttt tggctctgca gcggtggttt
    18301 ggcctcgcga ggctttgtat tcggcttttg agtagagttt cacttccgca tcttcacgtc
    18361 ccggcgtgca catgttgaac tgcagaatca gggtgcggaa aaccacgaag tacagcaggg
    18421 taaagcacaa cccaatggcg atttgggtca gcatcatatc cgcgtggttg ctgaacatcg
    18481 ggatccagtt ttgcggtaaa acctggtcaa tcagacctcc gcccatgttg cccaccacac
    18541 caaagagata cattacggtc gacattgagg ccgccagcac ggcgtgtacc gcaaacagca
    18601 acggtgaaat gaacaggaag gtaaattcca gcggttcggt aattcccacc agcatggcgg
    18661 ttaaggttgc gggaatcagc aagcccgcca cttttacccg attttccggt gcggcagtga
    18721 agtacatcgc taaagaaatg cccacggcac caaagatttt tgagttaccg tgcagggcaa
    18781 aaccgccttc cgggaacaac gatttcagtg gctcggcgct cagactgaac tcttgcagat
    18841 gctgcgccca gtacatctga atgccgcctt caacagctgc cggaccaaag ataaacggtc
    18901 cgtagatgaa gtggtgtaaa ccggttggga tcagaatacg ttcgaggaag gtgtaaaccc
    18961 acaccccaag cgcacccgcc gaacgcagga acgcttgcag agattcaatc cccatttgta
    19021 cttttggcca gccgagcaat gtcagccagg cacaggggat catcaccagg aaggcgataa
    19081 tcaccacata agacgtcccc tggaaaatgc cgagaaaaac cggcagtttt ttatcgaaca
    19141 gacggttatg cagcgccgtc acaatgccgg aaatgataat tgcgccgata atgctggtat
    19201 cgagggtttt aatcccggcc atcattgtca gaccgctacc tgccaccgcg tcctgagtga
    19261 aatcgacgcc gaagtagctt ccccaggtca ttcccatcgc attgatgaaa tagttccagg
    19321 tcaggaaact caccatcacc gccagacaag cacgtccctg cgcttgctta gcaaggccaa
    19381 tgggtaaacc gacagcaaaa atcagcggca tattacggaa taccgtccaa ccgccctctt
    19441 caataatgtg tacgatttgc gcgaataaac tgttcggatc ggtcagtgat tccccgacaa
    19501 acatcgggtt ttgcagcaag atggcaagac ccaccacaat cccggcgaag ggaaacagca
    19561 gcactggcgt gaacatcgcg ccgccaaagc gttgaatttg actgagcatt tccacatcct
    19621 cttttctcaa ttctgtaagt aggaagcctg attggttatg cggcagacct ggagcgagga
    19681 taggaagatg cgatcggcag aacatgacga cccaatggga ttcgtgatcg ctttcatgct
    19741 tttcgttggg cggtagagat ctacgttttc gcgaaaaagt agacatctgt gagcacaggt
    19801 acacagcatc gaacacatct ttaaaaaaaa gatttttttc aatcgattaa gcagaacttg
    19861 tgggcgcatt acccgtgctt gcaggcaaaa aagagagcta gagatgatct acaaaagcat
    19921 tgcggagcgg ttaagaattc gacttaattc cgcggatttc acgctaaaca gccttcttcc
    19981 cggtgaaaaa aagctggcgg aagagtttgc ggtatcgcgg atgactatcc gtaaagccat
    20041 tgacctgctg gtagcgtggg ggctggtggt ccgccgccac ggcagcggca cttacctggt
    20101 gcgcaaagat gtgctgcatc aaaccgccag cctgaccgga ctggtggagg tgttaaaacg
    20161 gcagggaaaa acggtcacca gccaggtgct gatttttgaa atcatgcctg cgcctccggc
    20221 cattgccagc cagttacgga ttcaaatcaa cgagcagatc tacttctccc gtcgcgttcg
    20281 ttttgtggaa gggaaaccgc taatgctgga agacagctat atgccggtaa aactgttccg
    20341 taacctttcg ctgcaacatc tggaagggtc gaagtttgaa tatattgaac aagagtgcgg
    20401 gattttgatt ggcggtaatt atgaaagcct gatgccggtg ctcgccgata gactgctggc
    20461 gcggcaaatg aaggtagcgg aacacacgcc actgctgcgg atcacctcgt tgtcatatag
    20521 cgagagcggg gagtttttga attattcagt gatgttcaga aatgccagcg aatactaggt
    20581 ggagtaccat ttacggcgac tccacccgga aaaaagttaa ccgatactcc agaagagcac
    20641 cgccagtaat tggggggtga taatgcgcag gaacatcact aacggataga cagtggcgta
    20701 agagagcgcc gccgcaccac tggttggatg aagattatta gcaaacgcca gcgccggagg
    20761 atcggtcatg gaacctgcca gcatcccgca catggtcagg taattcattt tggctaacat
    20821 ccgcgccaga atgccaacag taatcagcgg aacggcggtg atcagggcac cataaccaat
    20881 ccagcttagc ccttcgccat tgaccagggt attcacaaaa tccccaccag atttcagacc
    20941 aacgaccgag aggaacagca cgatccccag ctcccgcagc gcgaggttgg cacttggcgg
    21001 cataaaccag tacagcttgc cgatactgcc gatacgcccg aggatcaacg ccataatcag
    21061 cggaccgccc gccagcccca gtttcaacgc ggccgggaat cctggcacaa agacgggaat
    21121 agaacctaac aatacgccaa gcccgatgcc aataaacact ggcagcatct gaacctgttg
    21181 cagtttttgc tgcgcattcc ccagcacatt ggcaacggca tcaattgcgg acggacgccc
    21241 caccaggttg aggatatcgc cgaactgcag gctgatatcg ccactggcga ccagttcgac
    21301 cccggcacgg ttcaggcgcg agataacaac gtcatagcgt tctttaaagt gcaggtcgcg
    21361 aatacgtttt ccgagcacgt tttcattggt gaccaccaca cgctcgacgc gcaaatcagt
    21421 gcctttcgta gacagcgagg tatcgacctc ctgaccaatc accagttgcg cattatgtaa
    21481 atccgctggc tgccccacca gatgcagcaa atcgcccagt tggataatgg tatctggcga
    21541 aggtactttt agggtttctt ctcgtttcag acgcgagcag ataattttgt cgccgttgag
    21601 aatcggtaca tctttaatgg ctaaatcatg caggttaggg ttctcaacgc gaatattgat
    21661 agtcttgatc agcgcgccgc cattggtgcg tgaagactcg tgctgctgag cttctgtctc
    21721 gacattgacg cggaaaataa cccgcaacat ccacatggtg aacaaaatcc cgcaaatgcc
    21781 gaatggatac gccatcgcgt aactcatccc catctgatcg accatttcca ttggtgtacc
    21841 caggtcgcgc aagatctgct gccctgcccc cagcgctggc gtattggtta ccgcaccgga
    21901 gaaaatcccc agcactaccg gcagtggaat atcaaacagt ttatgcagga tggcggtaac
    21961 cagaccaccg atgatgacga tcagaacagc aaacaggttg aggcgtaatc cggagacgcg
    22021 caatgaggca aagaagcccg gccctacctg aatcccgatg gtataaacga acaggatcag
    22081 gccaaattcc tgaataacat gcagcatatc gctacttaat gtcatccccg cctgagaaac
    22141 aaaatggccg acgatgatcc caccaaacag cacgccgcca atacctaatc ctatgccgcg
    22201 aaatttgacg ttgccgataa acaaaccgac gactgccacc aaagccagaa tactgaccgt
    22261 taatgctata tcactcatcg tccatttctc ttgagataac attttaattg ctacggatta
    22321 tggctgaatc ggtgaagcag atttgcctta tagcgcacat tatcacggga aagaacgtgc
    22381 cgaaatatct taaacagtcg aaatttaacg cattgttgaa gaagaaagac aaaaaaagcc
    22441 ccaccagaat ggcggggcaa agagaatagc tagttagcta tttaacgcgg gacgttcgct
    22501 gatagcgata cgctgcgctg cgatgggttc aggctcatta cgaattaaat caatatgcag
    22561 taaaccgttt acgaaggttg cgccagagac ttccatattt tcagccagcg taaaactcag
    22621 gctaaatggc tgattcataa gcccttgatg cagccatttt ttctcttctt ttggctgctc
    22681 cggcgtgcct tttacgctca ggcgcgtgcc ttccagttga atctctaaat cttcctgacg
    22741 gaaacctgcc agcgcaaggg taatgcggta gtggttatcg tcgcttttct caatgttgta
    22801 cggcgggaag ctctggcttt caccggcgtt ttgcagtgcg ttggccagtt tgtcaaaacc
    22861 gatccattga cgcatcagtg gggataaatc gaagttacgc atagtcattt ctccttctaa
    22921 gaagcgagta agtacctgca aatccgaaga ttcgcatatg ctccctgacg gcgagcatgg
    22981 agatgtcagg ccgcgccagg cggccttagg gaattagttg atttcgatac ggcgcggttt
    23041 tttcgcttcc ggaatcacgc gttcgagatc gatatacagc aaaccattta ccaggttagc
    23101 cccacgaaca tgaatgttct cagctaactg gaatttgcgt tcaaagttgc gttcagcgat
    23161 gccctggtac agataggtgc gctctttttg ttcgtcggcg tgagcacctt tcaccaccag
    23221 cagattatcc tgggcggtaa tttccagttc gctctcagca aaaccagcca cagcgatagc
    23281 aatgcggtaa tggttttcgt ctaccagttc aacgttatac ggagggtagc cgccattact
    23341 ctggctctgg ttgttttcta agtggttaaa caaacggtca aatccaatag cagaacggta
    23401 aagcggggat aaatcaaagt tacgcataat caatagctcc tgaaatcagc gagaatgtaa
    23461 gaccttccac aatggacagg tcaggtagcc agaacaccca tcaggcgcgt cctcatcgac
    23521 tacgatgtaa aatgggtctg gaaatgaact tttcaagcct tatcagcgga cttttttttg
    23581 cagtttatgg tctattgcat agactgaggg ggcagcacga aacgttaaag tgcgatagcc
    23641 gccacagtgc gacgtaatga tgatgttatt ttcagcaagg gatcgctata actcatcagg
    23701 ccaacaccca cgatcaacag atgaatttat tatgataaga aatgtgttgt tagcgttcat
    23761 gatatgcagc ggaatgacat tactcggagg atgctccagc gtgatgtccc ataccggggg
    23821 gaaagaagga acgtatcccg gcacccgcgc cagcgccacc atgattggcg acgatgaaac
    23881 caactggggc actaagtcgc tggcgattct cgatatgccg tttaccgctg taatggacac
    23941 gcttttactg ccgtgggatg tgttccgcaa agacagctcg gtgcgctcgc gcgttgaaaa
    24001 aagcgaagcc aacgcgcaag ccaccaatgc ggttattcca ccggccagaa tgcctgataa
    24061 ctgatttagc gatcagtttc ggtgatccac agctggccac cttccatcca cgccagccat
    24121 tgaccatccg gcgagaagac cacggcgtcc gcagaaggtg gattggcgtg gttttcggtt
    24181 aaatactcaa cctcgccgct ttgcgcatgg gcacaaacaa ttcgattatc cagcacaaag
    24241 cccaaccatt ctcctgacgg atgccagtta aatgcagact gaatatccgt tttgttatgg
    24301 gttaacttgc gcggttcgcc gccctgtggc gagataagcc acagttgcac aatgccgtta
    24361 tcatcacgca ttaaaaacgc gatttgcgta ccctgcggat tacagcgcac ccagtggcgc
    24421 gggacgttga ctaaccccgg ataagcccga tgatgggtaa aggttaaacg tcgctgcacg
    24481 acgccacgcg gtggcgcggg tagggttgtt tccgttccac ttaacggcgc atcacctgcc
    24541 gatttccagc tagcttcatc ttgcggtaac tcaacgataa atagctccgg cactttctcg
    24601 ccctttggcg aaagtgtgtc gccaataaac gccagcgcgt gatttcctac ccatccttct
    24661 tcataagcac gattgatttc atcactgcca ggctgcggcg tgggcgtggt tttactcacc
    24721 agcacacacc agtggctacc gctgtattca cgcggatgtt gcttttgtac gttgaccggg
    24781 ccaaacggtg cagcaacccc gacgtttcgc aaatccagcg ccggatcgag ttgatgcatt
    24841 acatggtcgt tataggtaaa gctccccctt tcaccgttcg ggctaaagac atgcacatgg
    24901 ctgccaccgc gcagcgctcc tggcgtatac ggcgcggtaa tatccattgc atcgagattg
    24961 ctcaccttgc cgccttcagc aatgactccg cgtcgatgat ggaagtcgta atgccatgtt
    25021 tcatcaggat tttcagggcc gtgaataaag acatatttct ctgacttcgg atgaacggtc
    25081 accacgccga cgtgtgcgcc ctgtgacgcg cgatagataa cctcgacctc gcctgtatgg
    25141 atattcacac gctcaatggt ctcgccggta aacgacgccc cagaaggacg cacgtcaaat
    25201 accagccact ggctgtcggg cgtccaggta ttggtatttg tattggtatt ggtattggtg
    25261 agcaggtgat tacggggagc aaaggttatc tgtttcatgg cgcggtcctg atacaaaacg
    25321 gttcccgcca tcatacttca caaggacttc actttcagac aatagtcttt tgccatcgtg
    25381 atgaatgtga gtgaaaagat ggaacatttc gacgtggcga ttattggcct cggcccggca
    25441 gggtcggcgt tggcacgaaa gttagccggc aaaatgcagg tgatcgcgct ggataaaaag
    25501 caccagtgcg gtactgaagg tttcagcaaa ccttgcggcg gtctgctggc accggacgcg
    25561 cagcgatctt ttattcgcga tggactgacg cttcctgtcg atgtgatcgc caatccacag
    25621 attttcagcg tcaaaactgt cgacgtcgcc gcatcgctca cgcgtaacta ccagcgaagc
    25681 tatatcaata ttaatcgcca tgctttcgac ttgtggatga aatcgctgat ccccgccagc
    25741 gttgaggttt atcacgatag cctgtgccga aaaatctggc gtgaggatga taaatggcat
    25801 gtcatttttc gtgcagacgg ctgggagcaa catattaccg cccgctatct ggtcggtgcc
    25861 gatggcgcaa actcgatggt gcggcgacat ctctacccgg atcatcaaat ccgtaaatat
    25921 gtcgctatcc agcagtggtt cgcagagaaa catccggtgc cgttctactc ctgcatcttt
    25981 gataatgcga taactgactg ttactcatgg agtatcagca aagatggtta ttttatcttt
    26041 ggcggtgcct atccaatgaa agacggtcag acgcgtttca cgacgctgaa agagaaaatg
    26101 agcgcctttc agttccagtt tggcaaagcg gtgaaaagtg agaaatgcac ggtgttgttt
    26161 ccctcacgct ggcaggattt tgtctgtggt aaggacaacg cctttctgat tggtgaagcg
    26221 gcgggattta tcagcgccag ctcgctggag gggattagct atgcgctgga tagcgcagag
    26281 attctgcgtg cggtgttcct gaaacagccg gagaagagca acgccgctta ctggcgcgcc
    26341 acacacaaac tgcgtttaaa actcttcggc aagatagtaa aaagccgatg cctgaccgca
    26401 ccggctttaa gaaagtggat tatgcgcagt ggtgtggcgc atattccaca gttgaaagat
    26461 tatccaacgc gcttcacatc acccaccagc aggatgtaag agagcgcgcc aatcaacgcg
    26521 acggcggaga tataaaccag tgcaggcgcg aaaccgtaac cctgcgccag gtaccccacc
    26581 accagcggaa cggtgatgcc gcccagacca ccggcgaagt taaacacgcc gccggttaaa
    26641 ccaatcaggc gcatcggtgc cagagaagag accagcgacc aggtaatcga agcaaaaccg
    26701 ttgccgaaga atgccagcgc catcaggcac ataatcatca tcggatcgtt ggtgtagtta
    26761 gcgcccataa tgcaggtgga gatcagcaag ccgcagataa tcggcgtttt acgcgcaaag
    26821 cccagtgaaa agcccttacg taccagcaga tccgcgaccc agccagagag caggacgccg
    26881 acaaacgccg cgaggaatgg caccgtggtc ataaagcctg ctttcagcgc cgtgattcct
    26941 ttttcctggg ttaaatagtt cgggaaccag gttaagaaaa accacagtgc agaagccacc
    27001 gcaaattgcc caagataaac gccgatcagt ttacgatgga acaccagttt ccagtctttg
    27061 gctgttaacg gctgacgcgc ctctttcttc accggcgcat caccatccac cagaccgccg
    27121 ccatcacgaa tgtaatccag ttcagctttg ctgatacctt tggtcaggcg cggcggctga
    27181 taaaccttaa accaaatcag cgaccagata atgccgatac caccagtgac aatgaacacc
    27241 cagtgccagc tcaacatctc ctgaatccag atcagcagcg gagtcaaaaa cgccagaccg
    27301 acaaactgac cagacgtata aaaaccaacg gcggaagcgc gttcatgttc cgggaaccag
    27361 ctggtcacca tccggttatt ggtcgggaaa gcgggcgctt cgaaaatacc ggttatcgca
    27421 cgcagaccaa ttaatgacat taatcccgtg gcaaagcctt ggaataaagt cgccactgac
    27481 caaccaaata tcgcaataaa ataagtcacg cgagaaccta cgcgatctaa aaaccaaccg
    27541 ccggggatct ggcatagcgt ataaagccag gcgaaggccg aaaatacata gcccatttcc
    27601 gctttcgtaa tgccgaactc ttcctgaata tgggcggaag ccacggccag gttagcgcgg
    27661 tcaacataac aaatgaccac cgtaataaag atcatcacca gcgtcagata acgccgacgc
    27721 cccggctttg ctgcattaac gggaatatcc atagcgagct ttctccagat tttgggcata
    27781 gcgaagccgc tcaccatgcc ctgtaattta cagagggtta tatttttgta ttgctgtttt
    27841 agtgcccgat gaggggctta cgtggcagga attaccactc tgctacgctg ttatcttcat
    27901 gacgccagag cggattacgc cagtccgggg catttttact gaactcaata actttagctt
    27961 cgtcgatttc cacacctaag cccggtttcg ttaacggttt aaagaagccg ccaaccatgc
    28021 tgaagtcttc tttgtttttc acaaagtcga gtaactccgc gcctttgttg taatgaatgc
    28081 ccatactttg ttactgaagt accgcgttat aggaaacgaa gtcgatatgc aggcaagccg
    28141 ccagtgcaat cggtccgaac ggacagtgcg gcgcaagggt cacatcatag gcttctgcca
    28201 ttccagcaat tttgtagcat tcggtaatac cgcctgcatg ggagagatcc ggttgcagaa
    28261 tcgaaatacc gcctgcctcc agcacgcgtt taaaatcgaa acgtgagaac atgcgctcac
    28321 ccgccgccag tggaatatgc gtttgtgccg ccaatttcgg gtagtattcg gcttgttccg
    28381 ccagcaccgg ctcttcaata aacagcgggc gatacggctc cagttcttta atcagcactt
    28441 tcgccattgg cgcgctgacg cgaccgtgga aatcaagacc aaactcaatc tgattgccaa
    28501 aagcttcacg aatttgtgcc acggtgttga ctgccgcatc taccgcgcgg gagttatcaa
    28561 ttagccccag ttcttcacaa ccgttcagtt tgaaggtatc gaagccgatt tcgcgcaggg
    28621 ttttaatgcc gtcgataaca tccgccggac gatcgccgcc gacccaactg taggctttaa
    28681 ttttgtcgcg aaccaggccg cccatcagtt gccagactgg cgcattcagc actttgcctt
    28741 tgatatccca taacgcctgg tcgatcccgg cgatggcgct catcaggatt ggaccgccac
    28801 gataaaatcc ggcgcgatac atcacttgcc ataagtcgtt gatgcgcgaa ggatcctgac
    28861 caatcaaata gtcacccagc tcgtgaactg ccgcttccac cgtacgggcg cggccttcaa
    28921 tcacgggctc gccccaaccg accacgcctt catcggtttc aattttcagg aacatccagc
    28981 ggggaggtaa acgatacgtg gtaattttgg tgattttcat tgcactgcct ctcgatacgc
    29041 cttaacaaat gctgctgcct gctgcgcggt gcgttctacg gactgcccgg cgcgataaag
    29101 atcgctgcct aagcccgccc caacacaacc tgcgtctatc cactgcgcca ggttttctgg
    29161 cgtcacgccg ccaacggcaa agactgcgat gtccggtggt aataccgctt ttaacgcttt
    29221 gatgtattgc ggaccaaaag ccgatgacgg aaatattttc agcgcctgcg cgcccgcttc
    29281 gagcgcggta aaggcttcgg tcgccgtcgc gcagccgggg caaacggtca tgccgtagcc
    29341 caccgcgcgg cggatcactt cactatggat attgggcgta acgatgagct gacagcccat
    29401 cctggcgagc gcatcgacct gttcaggttt cagtaccgta cctgcgccaa tcaacgcctt
    29461 gtcgccatac gcatcaacga tggcgggaat gctctgctcc cattgtgggg aattcagcgg
    29521 gatttcaacc gcgtcgaacc cggcgtcaat caccgcgcca acatgcgcca gcgcctcgtc
    29581 gggcgtaatg ccgcgcaaaa tggcgatcag cgggagttta gtttgccact gcatgagcga
    29641 tgctccttat accagcctga aatgccgtgt cgcccgccac cgccgccacg tcgcaaccca
    29701 tcgcctgaaa ggcttgctgg tagcgcgcgg tcagcgatgt tccggcgaca agggtgatgg
    29761 cgtgttgatg ggtcacatag tcgcgcatac tggcaacctc tgcgccaatc aacaaaccag
    29821 agagaaattc gctgacctgt tcgcggggaa gtgttcccag cacatgcgag gcgcgaactt
    29881 caaaaagctg cggcaatatg gcgggcgcat taaggccgcg ctcaaggcca gccgcgaagg
    29941 catcggcaga gttttcctgc ggcggcaaac ctgcgccaat caatgagtga tttaacagta
    30001 aatgatgtaa ttcaccggtc atcacggtgc gaaaatcgtt gatttgctgg ctatcggcct
    30061 gcacccattt gcaatgcgta ccgggcataa cataaagaga ggaaggagcc agagctcgcg
    30121 cgccgatcaa ttgtgtttct tcgccgcgca tcacattgtg gttatcgtca tgagagacgc
    30181 ataatccggg aataatccag atattgtcgc caacagacgt taattgttcg ccaatagacg
    30241 aaaaacgggc aggaacagat aaatacggag caactttcca gccgacattg ctgccgacca
    30301 ttcctgccat caccactggc gtattttctt cacgccagtc ggtcgtgact tctgctaaca
    30361 ctgcagccgg agattttccg ttcaggcgcg tgacgcctgc ttctgattgc ctgctctcca
    30421 ggcagtggtc gccctgataa agccaggcgc gcagattggt cgatccccag tcaattgcga
    30481 tgtagcgagc tgtcatgtga tttcctttaa ccttcgtgtc gagctggcaa tcatggtaag
    30541 cgccgcctgc tctgccgcat cgccgtcctg atgccgtatc gcatcgaaca gcgccttatg
    30601 ttcctggagc gtttgcggca tgttggcctc atcgcccatc caggttcgtt caaaaaccgc
    30661 ccgctgcagc gaactgatcg caatgctaag ttgctgtaac accgggttat gcaccgactg
    30721 cagcaccgcc tcgtggtagc gaatatccgc ttcgttaaac gcttcgcggt cctgattgtt
    30781 ggcaatcatc tcgttcagcg ccgattcaat ctgcgccaga tcgctggagg tcgcgcgctc
    30841 tgctgcccaa cgggcaatcg ccggttccac cagatttcgc acttcgctca tggcactgat
    30901 aagccgtggg tcgtagtcgt tttccagcac ccattgcagt acgtcagtgt cgaggtaatt
    30961 ccactggtta cgcggtgcca caaacgcccc gcgataacgt ttcatttcaa tcagccgctt
    31021 cgccatcagc gaacggaaca cctcacggat gatgttgcgc gaggttgcaa actcttcaca
    31081 gagctccgcc tcagccgcaa gtggcgagcc aggtacgtat ttgccgtgaa cgatctgttt
    31141 acccagcgta atgacaatgc gatcggtttt attgagagtc atggagagtc cttgtgctct
    31201 gtatgttctt ctctacttta ccccgatcga tgcataacgc ggcaactttg tagtaccagc
    31261 gtgatgacgt tcgcgtttgc cgcgcgcgta atgtagtaca aacttatatt gttgcgctac
    31321 aatttagatc aaaaaaagaa caacacataa aaaaatgaca tgcgtcgggc agaaatctga
    31381 aaagggatat caggcgctaa acaggaggga aagaagagta tgctttcaac ggcttagcta
    31441 ctcgtttaaa ggattaatca tgaagttgaa ttttaaggga ttttttaagg ctgccggttt
    31501 attcccactg gcgctgatgc tttcaggctg tatctcgtat gctctggttt cccataccgc
    31561 aaagggtagt tcaggaaagt atcaatcgca gtcagacacc atcactgggc tatcgcaggc
    31621 aaaagatagt aatggaacaa aaggctatgt ttttgtaggg gaatcgctgg attaccttat
    31681 cactgatggt gccgatgaca tcgttaagat gctcaatgat ccagcactta accggcacaa
    31741 tattcaggtt gccgatgacg caagatttgt tttaaatgcg gggaaaaaga aatttaccgg
    31801 cacaatatcg ctttactact actggaataa cgaagaagaa aaggcactgg caacgcatta
    31861 tggttttgcc tgtggtgttc aacactgtac caggtcactg gaaaacctaa aaggcacaat
    31921 ccatgagaaa aataaaaaca tggattactc aaaggtgatg gcgttctacc atccatttaa
    31981 agtgcgattt tatgaatact attcacccag aggcattccg gatggtgttt ccgcagcatt
    32041 actgccagtg actgttacac tggacatcat tactgcaccg ctgcaatttc tggttgtata
    32101 tgcagtaaac caataatcag taagcgggca aacgcgttta tgctgtttgc ccgcccacag
    32161 attaattcag cacatacttc tcaatagcaa acgccacgcc atcttcaagg ttggattttg
    32221 tgacaaagtt cgccacttct ttcaccgaag gaatagcgtt atccatcgcc acaccgacgc
    32281 ctgcatattc aatcattgcg atgtcgtttt cctgatcgcc aatcgccatg atttcttccg
    32341 gtttaatacc taacacgtcg gccagtgatt tcacacccgt acctttgtta acgcgtttat
    32401 cgaggatttc gaggaagtac ggcgcacttt tcagcacggt atatttctct ttcacttcct
    32461 gcggaatacg cgcgatagcc tggtcgagga tggcgggttc atcaatcatc atcactttca
    32521 ggaactgggt attggggtcc attttctccg cttcgcagaa caccagcgga attgtggcaa
    32581 cgaaggattc atgcaccgtg tagtagctga tatcgcggtt agcggtgtac agcgtggtgc
    32641 ggtccagggc gtggaaatga gaaccgactt cgcgagagag tttttccagg aaacgatagt
    32701 cgtcatagct gagagcggtt tgcgccacgg tgctaccatc agcggccttc tgtaccagcg
    32761 cgccgttata agtaatgcag tagtcgcccg gctgttccat atgcagctct ttcaggtagt
    32821 tgtgcacacc tgcatacgga cgacccgtcg ttagcacgac attcacgcca cgggcgcgag
    32881 ctgcggcaat cgcattttta acggcgggtg aaatggtgtg atcgggcagc agaagggtgc
    32941 catccatatc gatagcaatg agtttgatag ccatgagttc ccaaggtaga ttagttcctg
    33001 acccatgcta acgcgattcc gctcaaaaat cagtacaaca cccgagggaa aaggggatgc
    33061 aacgcgggtt gcatgctccc catttgctta gcggaagagt ttccctttca gcagttccat
    33121 gcctgcggaa agcagatcgt tattggcttg tgctgacact tcaccttgcg gtgagagcgc
    33181 atcaataatc ttcggcaatt gctctgccag taaactggaa gccgtactgg tatccacgcc
    33241 aagtttttgc ccgagatcgg acaccgcatt tgtgccgagc gccgactcca gttgctcgcc
    33301 gctaactgat tgatttctct gttgattact caaccaggtt gagagaatgg cccctaagcc
    33361 gccactttgc agtttttcca gcagcacctg aatgccaccc tgctcctcaa cccaacttaa
    33421 aatcgcctga tatttccccg catcgccttt cagaaaggca ccgacaactt catcaaaaag
    33481 ccccatgata atcacctgta aagcgttacg tgttgaccca aaaagtatag atctgcggga
    33541 tgataattgc ggattgcaga aataaaaagg gcggagatga tctccgccct tttcttatag
    33601 cttcttgccg gatgcggcgt gaacgcctta cccggcctac aaaatcatgc aaaatcaata
    33661 tattgcagga gtctcgtagg actaataagc gtagcgcatc aggcacgctc gcatggttaa
    33721 cgccattaaa tatcaatatt cgccgctttc agggcgttct cttcaataaa cgcacgacgc
    33781 ggttctacgg cgtcgcccat cagcgtggtg aacaactggt cagcggcaat cgcatcctta
    33841 acggtaacgc gcagcatacg acggctttcc gggtccatgg tggtttccca cagctgttcc
    33901 gggttcatct cgcccagacc tttataacgc tggatggaga ggccacgacg ggactctttc
    33961 accagccagt ccagcgcctg ctcgaagctg gctaccggct gacgacgctc gccacgttcg
    34021 ataaacgcat cttcttccag caagccacgc agtttctcac ccagcgtgca gatacgacga
    34081 tattcgccac cggtgataaa ctcgtgatcc agcggatagt cagtatccac accgtgggta
    34141 cgcacgcgaa caatcggctc gaacaggttt tgttcggcat tggtgtggac atcaaacttc
    34201 cactggctgc cgtgctgttc tttgtcgttc agttcgctga ccagcgcgtt cacccagcgg
    34261 gtaacggtct gctcatcaga aaggtcagct tccgtcaacg tcggctgata gataagctct
    34321 ttcagcattg ctttcggata acgacgctcc atgcggttga tcattttctg cgtcgcgttg
    34381 tactcagata ccagtttctc taacgcttcg ccagccaatg ccggtgcact ggcgttggtg
    34441 tgcagcgttg cgccgtccag cgcgatagag atctggtact gatccatcgc ttcgtcgtct
    34501 ttaatgtact gttcctgctt gcctttcttc actttgtaca gcggcggctg agcgatgtag
    34561 acgtgaccgc gttcaacgat ttccggcatc tgacgataga agaaggtcaa cagcagcgta
    34621 cgaatgtgcg agccgtcgac gtccgcatcg gtcatgatga tgatgctgtg ataacgcagt
    34681 ttgtccgggt tgtactcgtc acgaccgata ccacagccaa gcgcggtgat aagcgtcgcc
    34741 acttcctgag aagagagcat cttatcgaag cgcgctttct cgacgttaag gattttaccc
    34801 ttcagcggca gaatcgcctg gttcttgcgg ttacgcccct gcttcgcaga gccgcccgcg
    34861 gagtcccctt ccaccaggta cagttcggaa agcgccggat cgcgttcctg gcagtctgcc
    34921 agtttgcccg gcaggccagc taagtcgagc gcacctttac ggcgggtcat ttcacgcgcg
    34981 cgacgcgccg cttcacgggc acgggcagca tcgataattt tgccaaccac gattttcgca
    35041 tcagttgggt tttccagcag gtattccgcc agcagttcgt tcatctgctg ttcaaccgcc
    35101 gatttcacct cagaagaaac cagtttgtct ttggtctggg aggagaattt cgggtccggc
    35161 acttttacgg aaacgaccgc aatcaggcct tcacgcgcat cgtcaccggt ggcgctgact
    35221 ttggcttttt tgctgtagcc ttctttgtcc atgtaggcat tcagggtacg ggtcatcgcc
    35281 gcacggaagc ctgccaggtg agtaccgccg tcacgctgcg gaatgttgtt ggtaaagcag
    35341 tagatgtttt cctggaagcc atcgttccac tgcagcgcca cttcaacgcc aataccgtct
    35401 ttttcggtgg agaagtagaa gatatttggg tggatcggcg ttttgttctt gttcagatat
    35461 tcaacgaacg ctttgatgcc gccttcatag tggaagtggt cttctttgcc gtcacgcttg
    35521 tcgcgcagac gaatggaaac gccggagttg aggaacgaca actcacgcag acgtttcgcc
    35581 agaatttcat attcgaactc ggtcacattg gtgaaggttt cgaggctggg ccagaaacgc
    35641 accatggtgc cggttttttc agtctcgccg gtaaccgcca gcggagcctg cggtacaccg
    35701 tgttcgtaga tctgacggtg aattttaccc tcgcgctgaa taaccagctc cagtttttgc
    35761 gacagggcgt ttactaccga aacaccaacg ccgtgcagac cgccggacac tttataggag
    35821 ttatcgtcaa atttaccgcc tgcgtgcaga acggtcatga tcacttccgc cgccgatacg
    35881 ccctcttccg ggtgaatacc ggtcggaatg ccgcgcccgt catcctgtac agagacagag
    35941 ttatcggcgt gaatggtgac gataatttct ttacagtgac ccgcgagcgc ttcgtcgata
    36001 gcgttatcta ccacctcgaa taccatgtgg tgcagaccgg tgccgtcatc cgtgtcgccg
    36061 atatacatac ccgggcgctt acgcaccgca tccagccctt tcaggacttt gatactggag
    36121 gagtcataag aattcgacat caacgtttct cgctcattta tacttgggtt aatccgttat
    36181 tttacccttt tccacggtaa acatcttcga attttcgtcc gacatgtcta taacgtgttc
    36241 agcactgatc gcgctgacaa agacctgtga ttgcgtcgct tttaagcggc tggcaagcaa
    36301 cccgcgacgc tcatcatcaa gctcagaggc aaaatcatct atcaggtaga gacaccgccg
    36361 cccgctttca cgagtgagga actctccttg cgccagacgt aaggcgcaca tcaacagctt
    36421 aagctgccca cgcgataagg tatcttccac cggcgcaccg tcggcgcgaa tgcgtaagtc
    36481 cgctttatgc gggccatgcg cggtgtaggt tagctggcga tcgcgttcaa aattacgttc
    36541 cagcacctca gcgtactctg tctctttctc ccagccgcgc tggaaagaga aagtcagaga
    36601 aaactcaggg agaaattgct tacaggtatc atccatatcg gccgcgatac cggcgctata
    36661 ctccgcgcgc caggtgctga tttgctccgc cagcgggatc agttctttat cccacgggcg
    36721 tagctgttcg taacgagtca cctggcgcag cgccgcattg cgctgcttga gcaatcgctt
    36781 gagattgctc caggcggtga aaaatccagg ttcgttgtga aagcatcccc agtcgaggaa
    36841 tgctcttctg tatttggggc cgccgttgag taaagtaaac ccttctggcg ttatcaactg
    36901 catcggcatc aggtgcgcca gttccgcgac tttatgcccg tccgtaccgt caatacggac
    36961 tttgctgtcg ccctgtttgt ctttggttaa gccaatcgcc gtctcgcgct cttcgccctg
    37021 taatcgcccg tggagaacaa atgcctcctg ctcatggcga atgacgcgac caatctgcaa
    37081 actgcgaaac gcccgaccat ggccgagcgt atagatggct tccagcacgc tggttttgcc
    37141 actgccgttg gcacctacca gaaagttaaa gccgggagat aaagcgagat ccgcggtttc
    37201 aatattgcgg aaatcgcgga tcaacaagcg ggtgagggac attacagtct cattggcatg
    37261 acaacatagg cagccgactg tgatgcggca tcttcaatct gcacgctgga aaccgaatcg
    37321 gtcagcatca tgcggacgtt ttcgcatttc agcgcgttca gaacatccag cacatagctg
    37381 acgttgaagc cgatttccat ctccgcaccg ctataggtaa cgtcgaggat ctcttccgct
    37441 tcttcctgtt ccgggttgtt ggcagtaatt ttcagctggt tttcgctgac atacaggcgc
    37501 acaccgcgga atttctcgtt agagagaatc gccgcgcgag caaacgcctg cttgagcaga
    37561 tcgcagcctg cttccagatg tttgtccggg ttcttcggca gaacgcgacg gtaatccggg
    37621 aagcgaccat ccaccagttt cgaggtgaaa ataaagtcgc caacgtgggc acgaatgtta
    37681 ttgctgccaa tctgcacacg cagcagattg tcgccgccgt cgagcatacg catcagttca
    37741 atcacgcctt tacgcggtac gatcaccgaa tggcttggca aagattgacc aattggcatt
    37801 gaacagaccg ccagacggtg gccgtcggtt gccacggtgc gcagttcttc accttcggtt
    37861 tcaaacagca taccgtttaa gtaatagcga acatcctgat gcgccatcga aaactgggtt
    37921 gcttcaatca gacgcttcat cgtcgcctgc ggcagggtaa attcgacttc gctctgccag
    37981 tcatcgaggt tcgggaaatc cgccgctggc agggtagaca gcgaaaaacg gctacgcccg
    38041 gagcgcacca gcatccgttc accttccagc tgcacggcaa tttccgcgcc ttcaggcaga
    38101 ccacggcaga tatcaaagaa tttgcgcgcc ggaacggtcg tcgctcctgg ctcgtgtggc
    38161 tgaaccagcg caacgcgtgc caccatttcc atctcgagat cagtaccggt cagcgacaac
    38221 gtaccgtcag caacctgtaa cagcagatta ccgagaatcg gtagcgtagg acgaccacct
    38281 aacggaccgc tcacctgttg tagcggtttt aataaatgct cacgttctac ggtaaatttc
    38341 ataggtttac gatgacaatg ttctgattaa atttgaaaaa tcttctttga tatcgtggct
    38401 ctcttcacgc aactgctcga tcttacggca ggcatgaagc accgtcgtgt ggtcacgccc
    38461 accaaacgca tcgccaatct ctggcagact gtggttagtc agctctttcg ctagcgccat
    38521 cgccatctgg cgcggacgcg ccaccgagcg ggatcgacgc ttggaaagga gatccgcgac
    38581 tttgatcttg tagtactccg ccaccgtctt ctgaatattg tcgatggtga ccagtttttc
    38641 ctgcaatgcc agcaagtcgc gcagcgcctc acgcacgaag tcgatggtga tcgcccgtcc
    38701 ggtaaagttg gcgttagcaa taacgcggtt cagcgcccct tccagctcac gtacgttaga
    38761 tcgtagacgc ttggcgataa agaacgccac ttcacccggc aaacgaatgt cattttcgtc
    38821 ggcctttttc atcaggatcg ccacacgggt ttccagctct ggcggttcga tcgccacagt
    38881 cagtccccaa ccgaagcggg atttcaaacg atcctcaacg ccgttgatct ctttcggata
    38941 gcgatccgag gtgagaatga tctgttgatt accttccagc agggcgttga aggtgtggaa
    39001 aaactcttcc tgagatcgtt ctttattagc aaaaaactga atatcgtcga tcagcagtgc
    39061 atctacggaa cggtagtagc gtttaaactc ttcgatcgcg ttgttttgca gggctttaac
    39121 catgtcctga acaaagcgct cggagtgcat ataaaccact ttggcattcg gcttgcgcgc
    39181 cataatgccg ttacccaccg catgcagcag gtgagtttta cccagacccg tgccgccata
    39241 aaggaacaac gggttatagg caccgccagg gttatccgcc acctggcaag ccgccgcgcg
    39301 cgccagttgg ttagatttac cttcaacgaa gttatcaaac gtgtgtttga cgtttacgtt
    39361 agaacgatag gtcggttctg ccggggccgg gacgttatcc caacctgagc gcgtagaagg
    39421 cgcagcacgt tgcggctgcg tttgcgccac ctgtgcaggg gccgcgacgt tgctcgtcac
    39481 tgccgcttgt ggcgtttgcg tcaccggttt ggtgccgact tcaaaacgca gctgtggggc
    39541 atccgctccg cagaaactgg ttagcagtcc attgatatta ttaaggtact tgtcccgtac
    39601 ccaatcgagg acaaaacggt ttggcgcgta cagggccagc gtgttatcgc tcagttccgc
    39661 ctgcaatggg cgtatccaca tactgaattc tgtggctggt aactcatcct gcaatcgggc
    39721 aagacactgc tgccaaagcg aaagtgacac ggcggactcc actcgaacaa aagtcgataa
    39781 tgactaaggc tgaaacattc atgattgttg acgtacgtcg aaaagaccct gcttgagggt
    39841 gacgcacgaa ccgctgtctg cggttatatg cccgatcaag atcctgcaaa acgatcggga
    39901 ccgcggatca tagcctaaac tgcgcaagag atcttctgtt tctcacagat tttccctatt
    39961 tatccacagg actttccaga actcgctaag tgtaaacgat cctgccgcga ggcgggcacg
    40021 atttacgccg catattggaa aatttaatga ccatagacaa aaattggctt aatcgatcta
    40081 ataaagatcc aggacgatcc ttgcgcttta cccatcagcc cgtataatcc tccacccggc
    40141 gcgccatgct ggtttccact ggtgtgaggt cgtacatttt ccctgcgaaa aggtgcggaa
    40201 aagcgcggta aataaggaaa gagaattgac tccggtgtgt acaattatta caatccggcc
    40261 tctttaatca cccatggctt cggtgtccat cgtttcattt ttcggcggat atccaataaa
    40321 gccattgaat ttattcaagt ttaggtagaa atcgccatga aacgcacttt tcaaccgtct
    40381 gtactgaagc gcaaccgttc tcacggcttc cgtgctcgta tggctactaa aaatggtcgt
    40441 caggttctgg cacgtcgtcg tgctaaaggc cgcgctcgtc tgaccgtttc taagtaataa
    40501 agctaacccc tgagtggtta agctcgcatt tcccagggag ttacgcttgt taactcccag
    40561 tcaattcaca ttcgtcttcc agcagccaca acgggctggc acgccgcaaa ttaccattct
    40621 cggccgcctg aattcgctgg ggcatccccg tatcggtctt acagtcgcca agaaaaacgt
    40681 tcgacgcgcc catgaacgca atcggattaa acgcctgacg cgtgaaagct tccgtctgcg
    40741 ccaacatgaa ctcccggcta tggatttcgt ggtggtggcg aaaaaagggg ttgccgacct
    40801 cgataaccgt gctctctcgg aagcgttgga aaaattatgg cgccgccact gtcgcctggc
    40861 tcgcgggtcc tgatagccct cattcgggtc tatcaacgcc tgattagtcc gctactcggg
    40921 ccgcattgtc gtttcactcc aacctgttca agctacggaa ttgaggcatt gcgcaggttt
    40981 ggagtgataa aaggcagttg gttgacggtg aaacgcgtat taaaatgcca ccctttacac
    41041 cctggtggtg acgatcccgt cccgcccgga ccatttgata ccagagaaca ctaacgatgg
    41101 attcgcaacg caatctttta gtcatcgctt tgctgttcgt gtctttcatg atctggcaag
    41161 cctgggagca ggataaaaac ccgcaacctc aggcccaaca gaccacgcag acaacgacca
    41221 ccgcagcggg tagcgccgcc gaccagggcg taccggccag tggccagggg aaactgatct
    41281 cggttaagac cgacgtgctt gatctgacca tcaacacccg tggtggtgat gttgagcaag
    41341 ctctgctgcc tgcttacccg aaagagctga actctaccca gccgttccag ctgttggaaa
    41401 cttcaccgca gtttatttat caggcacaga gcggtctgac cggtcgtgat ggcccggata
    41461 acccggctaa cggcccgcgt ccgctgtata acgttgaaaa agacgcttat gtgctggctg
    41521 aaggtcaaaa cgaactgcag gtgccgatga cgtataccga cgcggcaggc aacacgttta
    41581 ccaaaacgtt tgtcctgaaa cgtggtgatt acgctgtcaa cgtcaactac aacgtgcaga
    41641 acgctggcga gaaaccgctg gaaatctcct cgtttggtca gttgaagcaa tccatcactc
    41701 tgccaccgca tctcgatacc ggaagcagca acttcgcact gcacaccttc cgtggcgcgg
    41761 cgtactccac gcctgacgag aagtatgaga aatacaagtt cgataccatt gccgataacg
    41821 aaaacctgaa catctcttcg aaaggtggtt gggtggcgat gctgcaacag tatttcgcga
    41881 cggcgtggat cccgcataac gacggtacca acaacttcta taccgctaat ctgggtaacg
    41941 gcatcgtcgc tatcggctat aaatctcagc cggtactggt tcagcctggt cagactggcg
    42001 cgatgaacag caccctgtgg gttggcccgg aaatccagga caaaatggca gctgttgctc
    42061 cgcacctgga tctgaccgtt gattacggtt ggttgtggtt catctctcag ccgctgttca
    42121 aactgctgaa atggatccat agctttgtgg gtaactgggg cttctccatt atcatcatca
    42181 cctttatcgt tcgtggcatc atgtacccgc tgaccaaagc gcagtacacc tccatggcga
    42241 agatgcgtat gctgcagccg aagattcagg caatgcgtga gcgtctgggc gatgacaaac
    42301 agcgtatcag tcaggaaatg atggcgctgt acaaagctga gaaggttaac ccgctgggcg
    42361 gctgcttccc gctgctgatc cagatgccaa tcttcctggc gttgtactac atgctgatgg
    42421 gttccgttga actgcgtcag gcaccgtttg cactgtggat ccacgacctg tcggcacagg
    42481 acccgtacta catcctgccg atcctgatgg gcgtaacgat gttcttcatt cagaagatgt
    42541 cgccgaccac tgtgaccgac ccgatgcagc agaagatcat gacctttatg ccggtcatct
    42601 tcaccgtgtt cttcctgtgg ttcccgtcag gtctggtact gtactatatc gtcagcaacc
    42661 tggtaaccat tattcagcag cagctgattt accgtggtct ggaaaaacgt ggcctgcata
    42721 gccgcgagaa gaaaaaatcc tgattcggtg agttttcgct aaaataaggg cggtcagttg
    42781 accgcctttt ttcttttcgt agggcggata agcaccgcgc atccgccaca caaagcaaca
    42841 ggaacatcat gagcgataat gacactatcg tagcccaggc cacgcctccg ggacgtggcg
    42901 gcgttggcat cctgcgcatc tccggcttca aagcccgtga agttgccgaa accgtgctgg
    42961 gtaaactgcc taagccgcgc tacgccgatt atcttccgtt taaagacgcc gacggcagcg
    43021 tgctcgatca ggggattgcg ctatggttcc ctggcccgaa ctcgttcacc ggcgaagatg
    43081 tgctggaact gcaaggtcat ggcggtccgg tgatcctcga cctgctgtta aaacgcattc
    43141 tgaccattcc cggcctgcgg attgctcgcc ctggtgagtt ttccgaacgc gcgtttctta
    43201 acgataaact tgacttagcc caggccgagg cgattgccga tcttatcgac gccagttcgg
    43261 aacaggcggc ccgttcggca cttaactcgc tgcaaggcgc attctccgca cgggttaatc
    43321 atctggtaga agccctcacc cacttgcgca tttacgtcga agcggcaatt gatttccctg
    43381 atgaagagat cgatttcctc tccgacggca aaattgaagc ccagcttaat gacgtgatgg
    43441 ccgatctcga tgcagtgcgt gctgaagcac gtcagggtag tttgttgcgc gaagggatga
    43501 aagtagtgat tgccggacgt cctaatgccg gtaaatcgag cctgttaaac gcgctggcgg
    43561 gccgtgaagc ggcaatcgta accgatatcg ccggaaccac gcgtgacgtg ctgcgtgagc
    43621 atatccacat tgacggaatg ccgctgcata tcatcgacac tgccgggcta cgtgaggcca
    43681 gtgacgaagt ggaacgtatt ggtatcgagc gcgcgtggca ggaaattgaa caggccgacc
    43741 gcgtgctgtt tatggtcgat ggcaccacga cagacgccgt tgatccggca gagatctggc
    43801 cggaatttat cgcccgtctg cccgcgaaac tgccgatcac cgtggtacgc aataaagccg
    43861 atatcaccgg cgaaacgctg ggaatgagtg aagtgaacgg tcacgcgtta attcgtctct
    43921 cggcaaggac tggtgaaggc gtggacgtgc tgcgtaacca tctcaaacag agcatgggct
    43981 ttgacaccaa tatggaaggc ggcttcctgg cgcgccgtcg ccacttacag gcgctggaac
    44041 aggcagcgga gcatttgcag caaggtaaag cgcaactgtt gggagcctgg gcaggtgaac
    44101 tgctggcgga agagttgcgc ctggcacagc agaacttaag cgaaatcacc ggggaattta
    44161 cttcagacga cctgctgggg cggattttct ccagcttctg tattggtaag taaccgcgct
    44221 tacgaagccg tattctgact gtcagatgcg gcttcgcttc attgttagca ctcctgttat
    44281 gcctcaaccc ttttttaaac attaaaattc ttacgtaatt tataatcttt aaaaaaagca
    44341 tttaatactg ctccccgaac gattgtgatt cgattcacat ttaaacaatt tcagaataga
    44401 caaa
//
LOCUS       DAZPAB010000013        42940 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZPAB010000013 DAZPAB010000000
VERSION     DAZPAB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42940
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.013"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..1186
                     /locus_tag="ACHUPG_000641"
     CDS             <1..1186
                     /locus_tag="ACHUPG_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0860376.1"
                     /translation="IPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMFDKAIAVDS
                     SRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEGILLFYRNN
                     DMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRLAFLMTAPE
                     YACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDIIISNTGEK
                     FSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDTASANRVFH
                     QHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKNAECMAHIT
                     FTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYDRASGSASA
                     TQ"
     gene            complement(1246..1737)
                     /gene="yajQ"
                     /locus_tag="ACHUPG_000642"
     CDS             complement(1246..1737)
                     /gene="yajQ"
                     /locus_tag="ACHUPG_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR0860377.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            1905..2816
                     /gene="panE"
                     /locus_tag="ACHUPG_000643"
     CDS             1905..2816
                     /gene="panE"
                     /locus_tag="ACHUPG_000643"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR0860378.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            2779..3369
                     /gene="yajL"
                     /locus_tag="ACHUPG_000644"
     CDS             2779..3369
                     /gene="yajL"
                     /locus_tag="ACHUPG_000644"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR0860379.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3423..4871)
                     /gene="thiI"
                     /locus_tag="ACHUPG_000645"
     CDS             complement(3423..4871)
                     /gene="thiI"
                     /locus_tag="ACHUPG_000645"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR0860380.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5077..5319
                     /gene="xseB"
                     /locus_tag="ACHUPG_000646"
     CDS             5077..5319
                     /gene="xseB"
                     /locus_tag="ACHUPG_000646"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR0860381.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5319..6218
                     /gene="ispA"
                     /locus_tag="ACHUPG_000647"
     CDS             5319..6218
                     /gene="ispA"
                     /locus_tag="ACHUPG_000647"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR0860382.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6243..8105
                     /gene="dxs"
                     /locus_tag="ACHUPG_000648"
     CDS             6243..8105
                     /gene="dxs"
                     /locus_tag="ACHUPG_000648"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR0860383.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8160..9077
                     /gene="yajO"
                     /locus_tag="ACHUPG_000649"
     CDS             8160..9077
                     /gene="yajO"
                     /locus_tag="ACHUPG_000649"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR0860384.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(9188..9706)
                     /gene="pgpA"
                     /locus_tag="ACHUPG_000650"
     CDS             complement(9188..9706)
                     /gene="pgpA"
                     /locus_tag="ACHUPG_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR0860385.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9684..10661)
                     /gene="thiL"
                     /locus_tag="ACHUPG_000651"
     CDS             complement(9684..10661)
                     /gene="thiL"
                     /locus_tag="ACHUPG_000651"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR0860386.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(10739..11158)
                     /gene="nusB"
                     /locus_tag="ACHUPG_000652"
     CDS             complement(10739..11158)
                     /gene="nusB"
                     /locus_tag="ACHUPG_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR0860387.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11178..11648)
                     /gene="ribE"
                     /locus_tag="ACHUPG_000653"
     CDS             complement(11178..11648)
                     /gene="ribE"
                     /locus_tag="ACHUPG_000653"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR0860388.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(11737..12840)
                     /gene="ribD"
                     /locus_tag="ACHUPG_000654"
     CDS             complement(11737..12840)
                     /gene="ribD"
                     /locus_tag="ACHUPG_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR0860389.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(12844..13293)
                     /gene="nrdR"
                     /locus_tag="ACHUPG_000655"
     CDS             complement(12844..13293)
                     /gene="nrdR"
                     /locus_tag="ACHUPG_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR0860390.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13444..13983
                     /locus_tag="ACHUPG_000656"
     CDS             13444..13983
                     /locus_tag="ACHUPG_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR0860391.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14281..15165
                     /gene="tsx"
                     /locus_tag="ACHUPG_000657"
     CDS             14281..15165
                     /gene="tsx"
                     /locus_tag="ACHUPG_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR0860392.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15342..15689)
                     /gene="yajD"
                     /locus_tag="ACHUPG_000658"
     CDS             complement(15342..15689)
                     /gene="yajD"
                     /locus_tag="ACHUPG_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR0860393.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(15818..16789)
                     /gene="secF"
                     /locus_tag="ACHUPG_000659"
     CDS             complement(15818..16789)
                     /gene="secF"
                     /locus_tag="ACHUPG_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR0860394.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(16800..18647)
                     /gene="secD"
                     /locus_tag="ACHUPG_000660"
     CDS             complement(16800..18647)
                     /gene="secD"
                     /locus_tag="ACHUPG_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR0860395.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18675..19007)
                     /gene="yajC"
                     /locus_tag="ACHUPG_000661"
     CDS             complement(18675..19007)
                     /gene="yajC"
                     /locus_tag="ACHUPG_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR0860396.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19030..20157)
                     /gene="tgt"
                     /locus_tag="ACHUPG_000662"
     CDS             complement(19030..20157)
                     /gene="tgt"
                     /locus_tag="ACHUPG_000662"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR0860397.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLLEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20212..21282)
                     /gene="queA"
                     /locus_tag="ACHUPG_000663"
     CDS             complement(20212..21282)
                     /gene="queA"
                     /locus_tag="ACHUPG_000663"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR0860398.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21375..21948
                     /gene="acpH"
                     /locus_tag="ACHUPG_000664"
                     /pseudo
     CDS             21375..21948
                     /gene="acpH"
                     /locus_tag="ACHUPG_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(21953..23770)
                     /gene="malZ"
                     /locus_tag="ACHUPG_000665"
     CDS             complement(21953..23770)
                     /gene="malZ"
                     /locus_tag="ACHUPG_000665"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR0860399.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(23926..25299)
                     /gene="proY"
                     /locus_tag="ACHUPG_000666"
     CDS             complement(23926..25299)
                     /gene="proY"
                     /locus_tag="ACHUPG_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR0860400.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25375..26694)
                     /gene="brnQ"
                     /locus_tag="ACHUPG_000667"
     CDS             complement(25375..26694)
                     /gene="brnQ"
                     /locus_tag="ACHUPG_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR0860401.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27101..28396)
                     /gene="phoR"
                     /locus_tag="ACHUPG_000668"
     CDS             complement(27101..28396)
                     /gene="phoR"
                     /locus_tag="ACHUPG_000668"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR0860402.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28454..29143)
                     /gene="phoB"
                     /locus_tag="ACHUPG_000669"
     CDS             complement(28454..29143)
                     /gene="phoB"
                     /locus_tag="ACHUPG_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR0860403.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29333..30535
                     /gene="sbcD"
                     /locus_tag="ACHUPG_000670"
     CDS             29333..30535
                     /gene="sbcD"
                     /locus_tag="ACHUPG_000670"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR0860404.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            30532..33675
                     /gene="sbcC"
                     /locus_tag="ACHUPG_000671"
                     /pseudo
     CDS             30532..33675
                     /gene="sbcC"
                     /locus_tag="ACHUPG_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
     gene            33801..34982
                     /gene="araJ"
                     /locus_tag="ACHUPG_000672"
     CDS             33801..34982
                     /gene="araJ"
                     /locus_tag="ACHUPG_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR0860405.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(35125..36033)
                     /gene="mak"
                     /locus_tag="ACHUPG_000673"
     CDS             complement(35125..36033)
                     /gene="mak"
                     /locus_tag="ACHUPG_000673"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR0860406.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            36158..37069
                     /gene="rdgC"
                     /locus_tag="ACHUPG_000674"
     CDS             36158..37069
                     /gene="rdgC"
                     /locus_tag="ACHUPG_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR0860407.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37147..37602)
                     /locus_tag="ACHUPG_000675"
     CDS             complement(37147..37602)
                     /locus_tag="ACHUPG_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0860408.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37599..37934)
                     /locus_tag="ACHUPG_000676"
                     /pseudo
     CDS             complement(37599..37934)
                     /locus_tag="ACHUPG_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(38089..38373)
                     /gene="ppnP"
                     /locus_tag="ACHUPG_000677"
     CDS             complement(38089..38373)
                     /gene="ppnP"
                     /locus_tag="ACHUPG_000677"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR0860409.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(38445..39122)
                     /locus_tag="ACHUPG_000678"
     CDS             complement(38445..39122)
                     /locus_tag="ACHUPG_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR0860410.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(39380..39571)
                     /gene="yaiA"
                     /locus_tag="ACHUPG_000679"
     CDS             complement(39380..39571)
                     /gene="yaiA"
                     /locus_tag="ACHUPG_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR0860411.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(39621..40145)
                     /gene="aroL"
                     /locus_tag="ACHUPG_000680"
     CDS             complement(39621..40145)
                     /gene="aroL"
                     /locus_tag="ACHUPG_000680"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR0860412.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(40328..40786)
                     /locus_tag="ACHUPG_000681"
     CDS             complement(40328..40786)
                     /locus_tag="ACHUPG_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR0860413.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            40906..41715
                     /gene="proC"
                     /locus_tag="ACHUPG_000682"
     CDS             40906..41715
                     /gene="proC"
                     /locus_tag="ACHUPG_000682"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR0860414.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(41732..42847)
                     /gene="adrA"
                     /locus_tag="ACHUPG_000683"
                     /gene_synonym="dgcC"
     CDS             complement(41732..42847)
                     /gene="adrA"
                     /locus_tag="ACHUPG_000683"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR0860415.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        10861 a        11434 c        10678 g         9967 t
ORIGIN      
        1 tataccctat cagcaagtca ccatgttact tacagttgcg gagggttatc aaacaaaagg
       61 cgatataaaa aacgccattc attatgccga taaggcgctg aatatgtttg ataaagctat
      121 tgctgtagat agcagtcgtg aatcccatta tagtgatgag tatttgaaac tcatgaatat
      181 ctacgccgcc aatggcaaca aagaaaaagc ggtggtttta tctcaacgtc tacacaaagc
      241 cattaccgaa tctcaatcga attacttaag tactctggaa ggaattttat tgttttatag
      301 aaataatgat atgcagcaag actatcaaaa cagcctttca aattacatag tatttatcga
      361 taaaattttt gccttttccc catcaacaag aagtgaatta agcctcatta atttattaaa
      421 ttcattaaat gaggtggaat tgatgagaaa acgtctggcg tttctgatga cagcaccaga
      481 atacgcttgt tataatagtc agtattgtta tgaagacaaa gtcagcgcat taaaaatcct
      541 gtatcgtcat catgacaatg agctaatgga caaatatttc acgactcttc ttaaagagat
      601 cagtaatcaa ccattagaag actgggatat aataataagt aatactggtg aaaaatttag
      661 tgaagtgggt ctaaccaaat atgctcaaca tctcgcatct ttcgctgaag aattttatat
      721 aaaagagcaa aaatcacatt ctgaaagtga aatgagagga ctattttcat cacttgccga
      781 attatatagt tttggtaatg atactgccag cgcaaaccga gtgttccatc agcatgtccc
      841 tgccttcaat acagatcata tgattcaata ttttataaat gccaaagaat ggaacaatgc
      901 cagggaatta ttgataaaag aggaaatgtt gggtatgcat aatctgatat tgttggagaa
      961 tatttgtatg caaaaaaatg cagaatgcat ggcacacatt acctttacgc ttaataaatt
     1021 aacaacacaa ccagcaataa caaaaataga tagcgtgggt aatgagcaac tttatcagat
     1081 tggcaaaatc taccataagt tggaaattaa acctgagcca gagcaacagg ttttaattca
     1141 gtcgctttat gaccgggcat caggaagcgc ctctgcaact caataaatgc atggtcaaaa
     1201 gggccaccac ggtggccctg acaatcagta gctatatcca gaaagttaat cgcggaagtt
     1261 tttgaactgg aacggctgac cgagatcgcc accgcgcacc atcgccatga cagcctgcaa
     1321 atcatcacga gatttgccag tcacgcggat ctcatcgccc tgaatttgcg cctgcacttt
     1381 cagtttgctg tctttgatca tcttgacgat ttttttctga gtggcgctct caatgccctg
     1441 tttcagtttc gcttccacaa accaggtttt accgctatga acgatatttt ccggtacatc
     1501 cagtgaactg ccttcaatgc cgcgcttcag cagcttggca cgcagaatat ccagcaactg
     1561 attgacctgg aagtcggact cgctcaacac tttgatggtt ttgctggcgt cgttcagctc
     1621 aaatgaggct tcaacgttac ggaagtcaaa acgggactcc acttcgcgac tcgcgttatc
     1681 gaccgcgtta cgtgcttcct gaagatcaac ttcagagaca atatcgaaag atggcatctt
     1741 ttcctctccc ttcatttttg atgcgaagca taatacctgc aaagttaaat aaccaacagt
     1801 cctgcaccag gacgtatccc ttaattgtgc atagtcgcaa cagcatgcac aattaaggga
     1861 tagccttatt gaacacccgg agtggttgcg ggtgaggagg aacaatgaaa attaccgtat
     1921 tgggatgcgg tgccttaggg caattatggc ttacagcact ttgtaaacag ggtcatgaag
     1981 ttcagggctg gctgcgtgta ccgcaacctt attgtagtgt gaatctggtt gagacagatg
     2041 gttcgatatt taacgaatcg ctgaccgcca acgatcccga ttttctcgcc accagcgatc
     2101 tgctcctggt gacgctgaaa gcatggcagg tttccgatgc cgtcaaaagc ctcgcatcca
     2161 cactgcctgt aactacgcca atactgttaa ttcacaacgg catgggcacc atcgaagaat
     2221 tgcaaaacat tcagcagcca ttactgatgg gcaccaccac ccatgccgcc cgccgcgacg
     2281 gcaatgtcat tattcatgtg gcaaacggaa tcacgcatat cggtccggca cggcaacagg
     2341 atggcgatta cagttatctg gcggatattt tgcaaaccgt gttgcctgac gttgcctggc
     2401 ataacaatat tcgcgccgag ctgtggcgca agctggccgt caactgcgtg attaatccac
     2461 tgactgccat ctggaattgc ccgaacggtg aattacgtca tcatccgcaa gaaattatgc
     2521 aaatatgcga agaagtcgcg gcagtgatcg aacgcgaagg gcatcatact tcagcggaag
     2581 atttgcgtga ttacgtgatg caggtgattg atgccacagc agaaaatatc tcgtcgatgt
     2641 tgcaggatat ccgcgcgctg cgccacaccg aaatcgacta tatcaatggt tttctcttac
     2701 gccgcgcccg cgcgcatggg attgccgtac cggaaaacac ccgcctgttt gaaatggtaa
     2761 aaagaaagga gagtgaatat gagcgcatcg gcactggttt gcctcgcccc tggtagtgaa
     2821 gagactgaag ccgtcaccac gatcaacctc ttggttcgcg gcggtatcaa agtcaccact
     2881 gccagcgtcg acagcgatgg caacctggca attacctgct cgcgcggcgt gaagctgctg
     2941 gcggatgcgc cgctggtcga agtggctgat ggcgaatatg acgtgatcgt gctgcctggt
     3001 ggcattaaag gcacggagtg ttttcgcgat agcactctgc tggttgaaac cgttaaacag
     3061 ttccaccgtt ccgggcgtat cgtcgcggct atttgcgccg cgccagccac cgtgctggtg
     3121 ccgcacgata tctttccgat tggcaatatg accggcttcc cgacgctgaa agacaaaatt
     3181 cccgctgaac aatggcagga caagcgcgtc gtctgggatg cacgggtaaa attgctgacc
     3241 agccaggggc cgggtacagc tatcgacttt ggtctgaaaa ttatcgacct gctggttggg
     3301 cgcgagaaag cccatgaagt ggcatcacaa ctggtgatgg cggcagggat ttataattat
     3361 tacgagtagt gtcggatgcg gcaaacgtcg catctgacca gatgcgacgt aaaaactaca
     3421 aattacgggc gatatacctt cacattgtta aagccttgct cgcgcagata gagcgcctgc
     3481 agacggctca tcaccccgcg ctcacaccac agcagccagg ttttgttctg gtcgagatcg
     3541 ccaaatttgg tgctcagttt atagaacggc agagaaacca catcaatccc ttcgactttc
     3601 agtggcttat cttcctgttc atcgacagaa cggatatcga gaatcacgtc gttcgggccg
     3661 aagccgttga cggtttccac ttccaccact tcctgctcgg tctgctgggc gatttcgcgg
     3721 atatcaacgt tattcgcttc ctcaaccact ttatcgagaa tactgaagtc gaacttctct
     3781 tcttccgctt caatcttcga tttaaccgct ttcaccgtcg ggcttttgga gatcacgcca
     3841 caatattccg gcatcgtgcg agcaaagtct tcggtgccaa tctggcgagc caggttgatg
     3901 atgtgctctt tgtcgtaaga aatcagcgga cgcaggatca gcgtgtcaga gacgttatca
     3961 atcaggcgca ggttggtcag cgtctgactg gacacctggc cgagcgcttc gccggtgacc
     4021 agcgcctgta cgccgtaacg ttcagccact ttagatgcgg cacgcaccat catacgtttg
     4081 aggataacgc ccatctgacc gtcgtcgatt ttctcgagaa tttccccgac aaccggttcg
     4141 aaattaatgg cgacaaaacg cacgcggtgg gagctgccaa agcggttcca cagataatgc
     4201 gccacctgac gcacgccaat ttcatgcgct gcgccgccga ggttaaagaa gcagtaatgc
     4261 acgcggcagc cgcgacgcat caacatataa ctggaaacac cggagtcgaa accaccggaa
     4321 atgagcgaca gcacatcttc ctgggtgcca atcgggaaac caccaatacc ttcgtagcga
     4381 cctttaatca gcaggaggcg atcgtcttcc acttccagat gaacagttac atccgggtta
     4441 gtcagcttca cgcgcgcgga ttcaatatgc tgatttaaac cgccgccgac gtaacgttcc
     4501 acatcaatcg agctaaaatc atgtttgcca cggcgcttca cgcgtacgca gaaggttttg
     4561 ccttccagct gatcgcgata ctgaaccaac gctttctcaa aaatatcgtg catgtcggta
     4621 aacggcacgt cttcgacttc gagaatatgg tggatacccg gaatacgggt cagggcgtcg
     4681 cgaatagtca gacgctggtt ttcatctttc gcgcgaactt cgatgttatc ccagtggcgg
     4741 acgacagcga gcgtctcatc atagtgcttt aaaacgttac gaatgttccc ggtaaggatt
     4801 tttataaagc gcaagcgcac agattggctt ttgatggtga tttccgggaa caatttaatg
     4861 ataaacttca tggcggcaat ggttcgttgg caagccttaa gcgacttgta tagggaaaaa
     4921 tacagcagcc cacacctgcg gctgcatcca ggcgcggaag tataccacta acatcgcttt
     4981 gctgcgcaca tcaccttacc attgcgcgtt atttgctatt tgccctgagt ccgttaccat
     5041 gacggggcga aaaatattga gagtcagaca ttcattatgc cgaagaaaaa tgaggcgccc
     5101 gccagctttg aaaaggcgct gagcgagctg gaacagattg taacccgtct ggaaagtggc
     5161 gacctgccgc tggaagaggc gctgaacgag ttcgaacgcg gcgtgcagct ggcacgtcag
     5221 gggcaggcca aattacaaca agccgaacag cgcgtacaaa ttctgctgtc tgacaatgaa
     5281 gacgcctctc taaccccttt tacaccggac aatgagtaat ggactttccg cagcaactcg
     5341 aagcctgcgt taagcaggcc aaccaggcgc tgagccgttt tatcgcccca ctgccctttc
     5401 agaacactcc cgtggtcgaa accatgcagt atggcgcatt attaggtggt aagcgcctgc
     5461 gacctttcct ggtttatgcc accggtcata tgtttggcgt tagcacaaac acgctggacg
     5521 cacccgctgc tgccgtagag tgtatccacg cttactcatt aattcatgat gatttaccgg
     5581 cgatggatga tgacgatctg cgtcgcggtt tgccgacctg ccatgtgaag tttggcgaag
     5641 caaacgcgat tctcgctggc gacgctttac aaacgctggc gttctcgatt ctaagcgatg
     5701 ccaatatgcc ggaagtgtcg gatcgcgaca gaatttcgat gatttctgaa ctggcgagcg
     5761 ccagcggtat tgccggaatg tgcggtggtc aggcattaga tttagacgcg gaaggcaaac
     5821 acgtacctct ggacgcgctt gagcgtattc atcgtcataa aaccggcgca ttgattcgcg
     5881 ccgccgttcg ccttggtgca ttaagcgccg gagataaagg gcgtcgtgct ctgccagtac
     5941 tcgacaagta cgcagagagc atcggccttg ccttccaggt tcaggatgac atcctggatg
     6001 tggtaggaga tactgcaacg ttgggaaaac gccagggtgc cgaccagcaa cttggtaaaa
     6061 gtacctaccc tgcacttctg ggtcttgagc aagcccggaa gaaagcccgg gatctgatcg
     6121 acgatgcccg tcaggcgctg aaacaactgg ctgaacagtc actcgatacc tcggcactgg
     6181 aagcgctagc ggactacatc atccagcgta ataaataaac aataagtatt aataggcccc
     6241 tgatgagttt tgatattgcc aaatacccga ccctggcact ggtcgactcc acccaggagt
     6301 tacgactgtt gccgaaagag agtttaccga aactctgcga cgaactgcgc cgctatttac
     6361 tcgacagcgt gagccgttcc agcgggcact tcgcctccgg gctgggcacg gtcgaactga
     6421 ccgtggcgct gcactatgtc tacaacaccc catttgacca attgatttgg gatgtgggac
     6481 atcaggctta tccgcataaa attttgaccg gacgccgcga caaaatcggc accatccgtc
     6541 agaaaggcgg tctgcacccg ttcccgtggc gcggcgaaag cgaatatgac gtattaagcg
     6601 tcgggcattc atcaacctcc atcagtgccg gaattggtat tgcggttgct gccgagaaag
     6661 aaggcaaaaa tcgccgcacc gtctgtgtga ttggcgacgg cgcgattacc gctggcatgg
     6721 cgtttgaagc gatgaatcac gcgggcgata ttcgtcctga tatgctggtg gtcctcaacg
     6781 acaatgaaat gtcgatttcc gaaaatgttg gcgcgcttaa caaccatctg gctcagctgc
     6841 tttccggtaa gctttactct tcactgcgcg aaggcgggaa aaaagttttc tctggcgtgc
     6901 cgcccattaa agagctgctc aaacgtaccg aagaacatat taaaggcatg gtggtgcctg
     6961 gcacgttgtt tgaagagctg ggatttaact acatcggccc ggtggacggt cacgatgtgc
     7021 tggggcttat caccacgcta aagaacatgc gcgacctgaa aggcccgcag ttcctgcata
     7081 tcatgaccaa aaaaggtcgt ggttatgaac cggcagaaaa agacccgatc actttccacg
     7141 ccgtgcctaa atttgatccc tccagcggtt gtttgccgaa aagtagcggc ggtttgccga
     7201 gctattcaaa aatctttggc gactggttgt gcgaaacggc agcgaaagac aacaagctga
     7261 tggcgattac tccggcgatg cgtgaaggtt ccggcatggt cgagttttca cgtaaattcc
     7321 cggatcgtta cttcgacgtg gctattgccg agcaacacgc agtgaccttt gccgccggtc
     7381 tggcgattgg tggatacaaa cccattgtcg cgatctactc caccttcctg caacgcgcct
     7441 atgatcaggt gctgcatgac gtggcaattc aaaagctacc ggtcctgttc gccattgacc
     7501 gcgcgggcat tgttggtgct gacggtcaaa ctcaccaggg cgcttttgat ctctcttacc
     7561 tgcgctgcat accagaaatg gtcattatga ccccgagcga tgaaaacgaa tgtcgccaga
     7621 tgctctatac cggctatcac tataacgatg gcccgtccgc ggtgcgctac ccgcgcggca
     7681 acgcggttgg cgtggaactg acgccgctgg aaaaactacc aattggcaaa ggcattgtga
     7741 agcgtcgtgg cgagaaactg gtgatcctta actttggtac gctgatgcca gaaacggcca
     7801 aagtcgccga atcgctgaac gctacgctgg tcgatatgcg ttttgtgaaa ccacttgatg
     7861 aagcgttaat tctggaaatg gccgccagcc atgaagcgct ggtcaccgta gaagaaaacg
     7921 ccattatggg cggcgcaggt agcggagtga acgaagtgct gatggcccat cgtaaaccag
     7981 tacccgtgct gaacattggc ctgccggact tctttattcc gcaaggaact caggaagaaa
     8041 tgcgcgccga actcggcctc gatgccgccg gtatggaagc caaaatcaag gcctggttgg
     8101 cataatccct actccactcc tgctatgctt aagaaattat tcatggcagg agtggcagca
     8161 tgcaatacaa ccccttagga aaaaccgacc ttcgcgtttc ccgactttgc ctcggctgta
     8221 tgacctttgg cgagccagat cgcggtaatc acgcatggac actgccggaa gagagcagcc
     8281 gtcccataat taaacgcgcg ctggaaggtg gcataaattt ctttgatacc gccaatagct
     8341 attccgatgg tagcagcgaa gagatcgttg gtcgcgcact gcgggatttc gcccgtcgtg
     8401 aagacgtagt cgttgccacc aaagtgttcc atcgcgttgg tgatttaccg gaaggattat
     8461 cccgtgcaca aattttgcgc tctatcaacg acagcctgcg acgtctcggc atggattatg
     8521 tcgatatcct gcaaattcat cgctgggatt acaacacgcc gatcgaagag acgctggaag
     8581 ccctgaacga cgtggtaaaa gccgggaaag cgcgttatat cggcgcgtca tccatgcacg
     8641 cttcgcagtt tgctcaggcg ctggaactac aaaaacagca ctgctgggcg cagtttgtca
     8701 gtatgcagga tcactataat ctgatttatc gcgaagaaga gcgcgagatg ctgcctctgt
     8761 gttatcagga gggcgtggcg gtgattccgt ggagcccgct ggcgcggggg cgactgacgc
     8821 gtccatgggg agaaactacc gcacgactgg tgtctgatga ggtggggaaa aatctctata
     8881 aagaaagcga tgaaaatgac gcgcagatcg cagagcggtt aacgggcgtc agtgaagaac
     8941 tcggtgcaac acgagcacaa gttgcgctgg cctggttgtt aagtaaaccg ggcattgccg
     9001 caccgattat cggaacttcg cgggaagaac agcttgatga gctgctgaac gcggtggata
     9061 tcactttgaa gccataacag attgccgagc tggaaacgcc gtataaaccg catgcggtcg
     9121 taggatttaa ataagagtcc aggcctgatg agacgtgaca agcgtcacat caggcatcgg
     9181 tgcacaacta cgacagaata cccagcggcc agtgatgacc gataaagtac aggatgcctg
     9241 cggaaatcac cccggcgaca atatcgtcga tcatgatccc catgccgcca tgtacattgc
     9301 gatcaaacca gcggatcggc cacggcttcc acatatccag aatacggaaa atcacaaacc
     9361 cggcggcaac ccactgccag tcattggtcg gcagcgccat gagcgtgatc cacataccaa
     9421 taaattcgtc ccagacaatg ctgccgtgat cgtgcacgcc catatctttc gccgtttgat
     9481 gacaaagata gacgccgata cagatcccca acatcaccac cagcgagtag agctgccagg
     9541 gtaaaaaggt catcagatac cagaaaggga tcgccgccag cgatcccatc gagccaggaa
     9601 cgattgggct taatccactt ccgaatccga cagcaagcag atgccacgga ttactcatct
     9661 tcaggcgact tttcgcgaca tctttatggc gtgacaaaat ggtcatatcc tttccagtca
     9721 aacgtgacag gttcgccgtc acgaataaaa caaagccctt cgatatcggc ggtcatttgc
     9781 ccgatacagg taaacggtac gccaaggtgt ccgagagcaa catccagcgc gccacggttc
     9841 agttccggca ccgtgaaaca caactcgtaa tcttcaccgc cagagagcgc ccagcgcagc
     9901 gcctgttccg gttcaacatg gcgagaaagc gcatcagaaa acggcagcaa tgccaggtca
     9961 atacgtgcgc cgcagtcgct ggctttcacg atatgcccga gatcggaaat cagaccgtca
    10021 gagagatcga tagctgaatt tgccagatcg cgcagtgcct gcccctgtaa aatacgcggc
    10081 gatggacgga gatgacgttt gatcaagtag tcagcatctt tagcatcggc aacctgcaaa
    10141 cggttttgca aaatcgccag cccggcggcg ctatcgcccg gtgtaccggt cacatagatc
    10201 cagtcacccg gtttcgcccc agagcgcgtt aaggctcgtc ccatcggaac aaagccgtgg
    10261 atacccaacg tcattgataa tggcccacgc gtggtatcgc cgccaatgag ttgcatatcg
    10321 taataattga gaagatcaaa caaactgtcg ctgaaggact caagccacgc ttcgtctacg
    10381 tccggtaagg ttaatgccag cgtcagccag gccggatcgg cccccatcgc tgccagatcg
    10441 cttaggttca ccgccagtgc tttataagcc agatcagcag gatcgatatc agggaggaaa
    10501 tggttgcccg ccaccagcgt atcagtgctg atcgccaggg tctgtttctc ggggatattg
    10561 agaagagcgc aatcgtcgcc gatgcccagt tcgacatcaa gacgagaact tcttacacgg
    10621 tcaaaataac gggcaatcag ggagaactcg ccacatgcca tacgttatgc ctcagcaggt
    10681 aaaaaagaaa aggccggcga tcatggaaca gtcttccgtg aatctaccgg cctggatatt
    10741 actttttgtt agggcgaatc acaggtgctg ctttatcgag tacgccgttg acgaacttat
    10801 ggctgtcttc tgcgccgaac gatttcgcca gttcgatcgc ttcgttaatg gccactttgt
    10861 atggcacatc actacgttta gacagttcgt acagcgcaat gcgcagtact gctttttcta
    10921 cctggcccag ttcttccagc aggcgggaca ggtagggctt catcagtccg tcgaggtatg
    10981 cggtgttagt cgccaccccg gccagcagct cacggaagta caggacgtca acgtctttta
    11041 catcctgttc agccaggaac tggtattcaa catcagcgat gtcgttctgg gacaactgcc
    11101 aggagtagag cgcctggacg gcacactcac gagcgcggcg acgagcagca ggtttcacgg
    11161 atttcccctt actaatttca ggccttgatg gctttcaata cattaatcat ttcaagcgcg
    11221 gtcagtgcag cttctgcacc tttgttgcca gctttggtgc cagcacgttc gatcgcttgt
    11281 tcaatgcttt cagtggtcag aaccccaaaa gcaaccggaa tttcgctgtc ctgggcaaca
    11341 tgcgccaggc cgttgcttgc accaccagcg acatattcaa agtgggcagt gccaccacga
    11401 ataaccgtac ccagcgcaat caccgcgtcg tatttaccgg ttttagccag tgcacccgcc
    11461 gccagcggca gctcataggc accaggcacc caaacaacgg taatgttttc atcttttacc
    11521 tgaccgatac gtttcagtgc gtcaattgca ccttccagca ggctgtcatt gataaagttg
    11581 ttgaaacgcg cgatggtgat ggcgacgcga gcgtccgggg tagcaacgtt agcttcaata
    11641 atgttcattt tcttccttcg ggttcgagta tggccccgca ggggggcgga ttttagcata
    11701 atatttcgtg cgctgcttcc ctttcgagcc gggagatcat gcacccacta aatgcaggca
    11761 aacatccggg cctacatgac gtatctcttt gaatttaaat tggggggcgt cggctaattt
    11821 ctcaagccct ggcagcgagc ataatccacg ggcgtcgctg cctaatagtt taggtgcgat
    11881 atagacaatc agctcatcga ctaaacccgc ctgtagcaat acgccagcga gcgttggccc
    11941 cgcttccacc cagatgctgt taatctgctg tttacccagt tgcatcatca gtacaaccag
    12001 atccagatga cctttatgct ctggaatcag caaggtacgc accgtttccg gccactcacg
    12061 agaatcttcc tgcgtacgcg cgaaccaggt ttcgccgggc tgctgcacaa tgcgatgttc
    12121 cggcgtcacg cgattttggc tatcaatcac aatacgtact ggctggcgga gattttgttg
    12181 cgggtagagc gcctgagttt gttcatccag ttcagaccaa cgcactgtta aggctggatc
    12241 atccgccagc accgtggcgc tgctggttaa aatggcatga ctttgtgcgc gctgtcgttg
    12301 tacgtcgcgc cgcgcctgag gcgaagtgat ccactggctt tcgccgctcg ccatcgccgt
    12361 gcggccatca agcgatgcgc caagttttaa ctgaatataa ggaaagccgg tgcgcatccg
    12421 cttgagaaag cctttattca attgctcggc ttcactcatc atcagcccgt ggctgacgtc
    12481 aatgccagcc tgttgcagac ggtaaagtcc acgcccagcg acctgcgggt taggatcttg
    12541 catcgctgcg accacgcgcg ctacgccagc ggcgatgagt gcgtcacagc acggtggcgt
    12601 acggccatga tggctacagg gttcgagtgt cacatacgcc gttgcgcctt tggctttttc
    12661 acccgccata cgcaacgcgt gtacttcggc atgtggttca cccgctcgtt ggtggtaacc
    12721 ttcaccgaca atttcgccat ctttgacaat gacgcacccg acattcgggt tggggtgggt
    12781 ggtaaaacgt cctcgttgcg ccagctttag cgcccgcgcc atgtaatact cgtcctgcac
    12841 ggcttagtcc tccaggcgcg cgatctcttc gccaaattct ttgatatctt cgaaactgcg
    12901 gtagacagag gcaaaacgga tataggcgac tttatcgagc tttttcaatt gctccatcac
    12961 cagattgcca atcatcttgc tcggtacttc gcgctcaccg gtggcgcgca gctgcgattt
    13021 aatatgattg attgccattt caacgtaatc ggaactcacc ggacgttttt ccagcgcccg
    13081 cagcattccg ctacgcaatt tctcttcatt aaacggttca cgcacgtcgt tgctttttac
    13141 aacacgcggc ataaccagct ccgccacttc aaaggtggtg aaacgttcat tacacaccag
    13201 acactgccga cggcggcgta cggatgaacc ctcgcccacg agacgagagt caattacctt
    13261 agtgtccacg gcgaaacaga atgggcaatg catacggcgt cctgaccagg tggttaacgg
    13321 aaatctattt taccctgaac tgacgataca acaaaggcgc aacgcttttg agataaagga
    13381 tcgatgcttt cgctgctttt accctctacc attaggctaa ttgcgacgag aaagggacga
    13441 cctatgaata ctaacgtttt tcgactgctc ctgctgggaa gcctgttcag ccttagcgcc
    13501 tgtgtgcagc aaagtgaagt gcgacagatg aaacacagcg tcagcacgct gaaccaggag
    13561 atgacgcaac tcaatcaaga aacagtcaaa atcactcagc aaaacaggct gaatgcaaaa
    13621 tccagcagtg gggtttacct tctgcctgga gcgaaaacac cggcaagact ggaaagccag
    13681 atcggtactt tacgtatgtc gctggtgaat attacgcctg atacagatgg caccaccctt
    13741 acactacgta ttcagggtga gtcgaatgat cccttgcccg cattcagcgg aactgttgaa
    13801 tatgggcaaa ttcagggaac aatagacaac tttcaggaaa tcaatgtgca gaatcaattg
    13861 attaatgccc ctgccagcgt cctcgccccc agcgatgttg atattccgtt acaattaaag
    13921 gggatttctg tggatcagtt aggctttgta cgtatccacg acattcaacc tgttatgcag
    13981 taaacgtatt tcgggacgat tttgtgcgtc ccgcaacatc ttccccgtca ttttgttact
    14041 ctgcttacat cacctggatt gatagtaaaa gtttgcaaca agggcgaaag tcagtacaat
    14101 ccccgcccga atgtgtgtaa acgtgaacgc aatcgattac gtaaatgata gaactgtgaa
    14161 acgaaacata tttttgtgag caatgatttt tataataggc tcctctgtat acgaaatatt
    14221 tagaaacgca atttgcgcct ttttcactcc cgcaagggat tttcaaacag tggcatacat
    14281 atgaaaaaaa cattactggc agccggtgcg gtactggcgc tctcttcgtc ttttactgtc
    14341 aacgcagctg aaaacgacaa accgcagtat ctttccgact ggtggcacca gagcgttaac
    14401 gttgtcggaa gctatcacac ccgtttcgga ccgcagatcc gcaacgatac ctaccttgag
    14461 tacgaagcat tcgctaaaaa agactggttc gacttctatg gttatgcgga tgcgccggta
    14521 ttcttcggcg gtaactccga tgctaaaggt atctggaacc acggttctcc gctgtttatg
    14581 gaaatcgaac cacgtttctc catcgacaag ctgaccaata ctgaccttag cttcggtccg
    14641 ttcaaagagt ggtacttcgc gaacaactac atttacgaca tgggtcgtaa taaagatggt
    14701 cgccagagca cctggtacat gggtctgggt accgatatcg acactggcct gccgatgagc
    14761 ctgtccatga acgtctatgc gaaataccag tggcagaact atggcgcagc gaacgaaaac
    14821 gagtgggacg gttaccgttt caaaattaaa tactttgtgc cgattaccga tctgtggggc
    14881 ggtcagctga gctacatcgg cttcaccaac ttcgactggg gttccgattt aggggatgac
    14941 agcggtaacg caatcaacgg tattaagacc cgtactaata actctatcgc ttccagccat
    15001 attctggctc tgaactacga tcactggcac tactctgtcg tagctcgtta ctggcacgac
    15061 ggtggtcagt ggaacgacga tgcagaactg aacttcggca acggcaactt caacgttcgc
    15121 tctaccggct ggggtggtta cctggtagta ggttacaact tctgattatg aaaatgccgg
    15181 gatttattcc cggcatttct gattgttaac aacgtgtgct ttgttcatgc cggatgcggc
    15241 gtaaacgctt tgtccggccc acaaaagcac gtaaattcaa tatattgctg aaataacgta
    15301 ggcctgataa gcgtagcgca tcaggccatt ttacgtttta atcacttctt cttgttcatc
    15361 atcgctttca ggtcagcgaa tgggttgtac ttcgcttcac cgacatcttt ctgcgcatct
    15421 tcccctgcga taacggtcgt accatactga tccgcttcgg tatatttcga atgctcatga
    15481 tcgtggcaat agagacacaa caattcccag ttactgccat cttccgggtt attggtatgg
    15541 tcgtgatcaa tgtggtgaac ggtaagttca cgcaggttag aataaacaaa ctcgcgggaa
    15601 cagcgaccgc agacccacgg atagattttt aatgcttttt cgcgatagcc gctttctaac
    15661 cgtgcatagt tttttgggat gatagccatg aaaagttacc tggatgaaat gtagggttac
    15721 gcgcagtttc ccaagatgcg gggattttcg caatctccat acatcaaaaa aaatcccgat
    15781 cttctgaccg ggattttcaa catcaacggg aacttgatta aggcagaatt gacggctgat
    15841 ccgccccttc tttttccact ttctgctgca acatgtgttc gcgcttcata cccagtttca
    15901 gagccaacgc agatgctacg tagatggaag atgcagtgcc gatggaaaca ccgataagca
    15961 tggtcagcga gaagccttcc agtaccggac caccgaagag atacagcatc aggataacca
    16021 tcaaggtagt accggatgtg atcaaggtac ggtgcagcgt ctgggtcaag gacacgttaa
    16081 agatttcgta aggcgtaccg cgacggatct tgcggaagtt ttcacgaatt cggtccgata
    16141 ccacgatact gtcgttaagc gagtaaccga taaccgacat caacgatgcc acaatggtca
    16201 ggtcaatctc gatatggaat aacgacaaaa tacccagcgt aatgataacg tcgtgcgtca
    16261 gcgcaataac cacccctgcc gccagtcgcc actcaaagcg gaaacctacg tacacgagga
    16321 tagacagcag cgctgccatc aacgccatcg caccggtttg cgcaaggtca gcccccacgc
    16381 tcggaccgac gaactcaata cgcttcactg ctgcattctg attggtggat tcgttaatca
    16441 ccttcagaac ttggctgccc agcacctgac cgccggtttc gccttcagca ggcggcatac
    16501 ggaccatgat gtcgtggctg ctaccaaagt tttgcagcat tggctcttca aaaccggctt
    16561 tttgcaatgc atcacgcatt acgtcaattt cagccggttt ttcgagcgta atttcgataa
    16621 ccgtaccacc ggtgaaatcc agcccccagt taaagccgcg cacgcccata ataacgatag
    16681 cagcgattaa cagcagacca gagatgccga aagcccagta gtcccagcgc ataaagtcat
    16741 agactttacg gccgtggttt agttgttcaa cagtatattc ctgtgccaca tcgcactcct
    16801 cagattgaca gcttcttgac gcgcttgccg ccatatagca ggtttacgat ggcacgcgta
    16861 ccgacaatcg cggtaaacat cgacgtcgcc acaccgatac cggtagtaat cgcgaaccct
    16921 ttaattgccc cggtacccac tgcgtacagg atgatgactt taatcagcgt ggtgatgttc
    16981 gcatcgaaga tagaactgaa tgcgccacga taaccttcat caattgcctg ttgaacagta
    17041 cgtccgttgc tcaactcttc tttaatacgt tcgttgatca gtacgttcgc atcgaccgct
    17101 accgcaaggg ttaagacgat acccgcgata cctggcatac tcagtgttgc gcctggcaac
    17161 agcgacataa taccgacgat caagatcaag ttggcaatca gagcactggt cgcaatcaga
    17221 ccaaacttct tatagaagat gatcatgaac agaatagaaa ccagcagacc ggcaaggcaa
    17281 gcctccagcc cctgctcaat gttctgcata cccagggtcg ggccaattgt acgttcttca
    17341 acaatctgaa tcggcgcgat caacgcaccg gcacgcagca gcagtgacag ctgacgggct
    17401 tcgttcgggt tgttgatgcc ggtgatacgg aagctgttac ccagacgaga ctggatgttg
    17461 gcgatgttaa tcacctcttc ctgtttcacc agaaccgcac gaccatttgc atctttctta
    17521 ccgctgtctt tgtactccac aaacagggtt gccatcggtt tgccgatgtt gtccttggtg
    17581 aagttagaca taatgttgcc accagcgcta tcgagcgaga tgttaacctg cggctggttg
    17641 tattcgtcct ggctggaagt ggagtcggtg atatggtcac cagtcagaat tacgcgtttg
    17701 tacagcacaa ccggctgacc ttcgcgggtc tgtttcactt cagagtcgcc cggtacgcga
    17761 ccggatgccg cagcggcctg atcaacattg gtgtttacca gacggaattc cagcgttgcc
    17821 gtcgcgccca gaatctcttt cgcacgcgca gtgtcctgaa taccgggcag ttcaacaacg
    17881 atacggtcag caccctgacg ctgaaccacc ggctccgcca cgccaagttg gtttacacgg
    17941 ttacgcagga tattaatgtt ctgctgcacc gcatattcac gcgcttcact cagacgagca
    18001 tcgctcatta ccgcacgcag ctggttgctg ccctggctgc taatcaccag gtccggatgg
    18061 cgcttgctca gatacgcaat ggcttcatca cgagctttag catcgcggaa agtgatgctc
    18121 aggccgtagt tgttttcttt acgaacagtg gtatacggga tgcccttttc gcgcaggtca
    18181 ctgcgtaggc tatcgatatt ttgttcctgg agtttgccaa gcgcggtgtc catatccact
    18241 tccatcagga agtgaacgcc gccacgcagg tcaaggccga gcttcatcgg ctcagcatga
    18301 atagctgcca gccagcgcgg cgttgccggg gcaaggttaa gcgccacgac gtatttgtca
    18361 cccataacgc ccattaatgc ttcacgagcg cgcaactggg tgtcagtgga gtcgaagcgc
    18421 gcaagaatag cgccctcttc cagtgccaca gacttagcag ttattttttc ttcttgtaac
    18481 gttttctgga cctggatcag cgtttgctca ctggcggcga ctccgcgcgc accagtgatc
    18541 tgaacagccg gatcctcacc aaacaggttg ggaagcgcat acagcagacc gatgacaatc
    18601 accacgatca gcatgacgta cttccacaaa ggataacggt ttaacacggc aattccctta
    18661 gggaaaaatt ttaattacag cgccttcatg gtgcctttcg gcaggacggc agctacgaag
    18721 tcacgtttaa taactacttc agtggtgtca ttcagcgcga tagcaatgta gccgttttcc
    18781 gctactttgg ttacgcgacc aaccaggcca ccgttcgtca gaacttcatc acctttggca
    18841 atggagtcca tcagcttttt gtgttctttg gtgcgcttct gctgtggacg caggatcatg
    18901 aaatagaaaa tcagaccgaa taccaccagc atcaaaatca aagacatcgg gctaccttgc
    18961 gccggtgcac ccgttgccgc taccgcatca gaaataaaaa agctcattaa atttccctca
    19021 ttattaatat taatcaacgt tcaaaggtgg tacttctcgc ccctgacgct ggtaaaaatc
    19081 agttacgaag ctctctaatt taccctcttc aatagccttg cgtaaacccg ccatcaaacg
    19141 ctggtagtaa cgaaggttat gaatggtgtt gagtcgcgcg cctaatattt cgttgcaacg
    19201 gtcgagatga tgcaagtaag cgcgtgaata attgcgacag gtgtagcaat cacactcagg
    19261 atcgagtggg ccagtatcgc tcttatattt cgcattgcgg attttcacca cgccatcggt
    19321 cacgaacaaa tgaccatttc gggcgttgcg ggttggcatt acgcagtcaa acatatcgat
    19381 accgcgacgt acgccttcaa gcaggtcttc cggtttacca acgcccatca ggtaacgcgg
    19441 tttgtctgcc ggaatttgcg gacacacatg ctccagaata cggtgcatat ctgctttcgg
    19501 ctcacccaca gccagaccgc cgacagcgta gccatcaaag ccgatatcta ccagaccttt
    19561 aacagatata tcacgtaaat cttcgtaaat actgccctga attataccaa ataacgcatt
    19621 tttgttacca agactgtcaa aacgctcacg gctacgcttc gcccaacgca gagacatctc
    19681 catagagcgt tttgcgtaat cccagtcagc aggatacggc gtacactcat caaagatcat
    19741 gacgatatcc gaaccaagat cgtactgaat ctccatcgac ttttccgggt cgaggaaaat
    19801 cgggtcgccg ttgatcgggt tacggaagtg aacgccctgt tcggtgattt tacgaatatc
    19861 accaaggctg aagacctgga agccgccgga gtcggtaaga atcggtcctt tccactgcat
    19921 aaaatcgtgc agatcgccgt gcagtttcat gatttcctgg cccgggcgca gccacaggtg
    19981 gaaggtgttg ccgaggataa tttgcgcgcc agtggcttca acttcttccg gcgtcatccc
    20041 ttttacggtg ccgtaggtgc caacaggcat aaaacaaggc gtttccacta cgccacgatc
    20101 aaagaccagg cggccacggc gtgcgcgacc gtcggtggtg tccagttcaa atttcatttt
    20161 ttctccacta cgtcagaaaa acagtccaac gtttaaacca gcgccgcgga attactcccc
    20221 gacgcgctca ttaattgcct gcggattgta cgtgataaac atcgcatcac cgtaactaaa
    20281 aaagcgatat ttctcttcta ccgccgcttt ataggcattc atggtgtgtt gataaccggc
    20341 aaaggccgaa accagcataa tcagcgtcga ctctggcaag tggaagttcg tcaccagcgc
    20401 atcgaccact ttgtactgga agcccggata gataaagatt tgggtatcgt caaagaacgg
    20461 ttcgataaga tcgttttttg ccgcctgtgc cgcgctttcc agcgaacgta ctgaagtggt
    20521 gccaaccgca atcacccggt taccgcgcgc tttcgccgcc agtaccgcgt ctaccacatc
    20581 ctgcggtact tcagcgtatt ccgagtgcat gatgtgatct tcaatggtgt cgacgcgcac
    20641 cggctggaag gtgcccgcac caacgtgcaa cgtcacaaac gccatctcca cgcctttggc
    20701 gcgtaatttt tccagcaaag gctcgtcaaa atgcagacca gcggtcgggg ctgcaaccgc
    20761 gcccggtttt tcgctataaa cggtttgata aagttcgcgg tcagcgtctt cgtccggacg
    20821 atcgatatac ggcggcagcg gcatatggcc gatgctgttg aggatatcca gtaccgagcg
    20881 ttcatcatta aattcgactt caaacagtgc gccgtggcgc gcggtcattg ttgcgttgat
    20941 actttcgtca tcgcccagca acagttctgc gccaggtttt ggcgctttcg aggcgcgaat
    21001 atgcgcaaga atgcgtttgt cgtcgagcat ccgctcaacc agcacttcaa tcttgccgcc
    21061 gctggcttta cgcccaaaca gacgcgccgg gatcacgcgg gtattattaa aaaccagaag
    21121 atcgccgggg ttgagcttat cgagtaaatc ggtgaaagta ccgtgcgtca gcgcgcccgt
    21181 cggcccgtcc agcgacagta aacgacagct actgcgttca ggcatgggat agtgggcaat
    21241 cagggattcg ggcaattcaa aggagaaatc ggtaacgcgc atgacactga ctcagactaa
    21301 aataagagtc gggtagtcta gtgccggggc gctctccctg caacctttac ccccctttgg
    21361 atagaatata atcgatgaat tttttagctc acctgcattt agcccatctc gcggaaagct
    21421 cgctttctgg caatttactg gctgatttcg tacgcggaaa tcctgaagaa agttttccgc
    21481 ccgacgtcgt ggctggcatt catatgcatc gacgtattga ctgacaatct gccggaagtc
    21541 cgcgaagcac aggagtggtt tcgtagtgaa acgcgccgcg ttgcgcctat tacgctggat
    21601 gtcatgtggg atcactttct ttcccgccac tggtcgcagc tgtcgccgga ctttccgcta
    21661 caggaatttg tctgttatgc ccgcgagcaa gtgatgacga ttttgccgga ctcaccgcca
    21721 cgttttatca atctgaacaa ttacttgtgg tcagagcagt ggctggtgcg ctatcgcgat
    21781 atggatttca tccagaacgt gttaaacggc atggcaagcc gccgcccacg tctggatgcc
    21841 ctgcgtgact cctggtacga tttagacgct cattatgacg ccctcgaaac ccgcttctgg
    21901 cagttttatc cgcggatgat ggcgcaggcg tcacacaagg cgttataaaa cgttagttca
    21961 tccataccgt agccgaaatg gcaggcaaag ccagaatccc gtcagtcagt tgcccatgcc
    22021 cttctttgca ttgccattgc acggcattga gaaacggtga cgcgggtagc accacttcac
    22081 tggcctcgcc acggttgatt gccaccagta cacgttgctg attcagcacg cggacaaata
    22141 ccactacgtt atcttccgca tacagcacct gacagccgcc acgacgcagc gcctgacttt
    22201 tcttacgcag cacaatcatt cgccggtaca gcgcgaataa cgccgtatcc tgcttttcca
    22261 cctgccaggg gaacggttta cggcaaaacg gatcgttttt accatccagt cccacttcat
    22321 caccgtaata aatgcacggt acaccaggcc aggtgaacag ccagaccacc gccagcggca
    22381 ggcgcgcaat atcccgaccg agcagcgttt taaatcgcgc agtatcgtgg ctatcgagct
    22441 gattaaacat acgtaattgt tgttgatgag aaagccctgc gcggtaatta tccatccagg
    22501 ccatacaggt ttgggcgtca atttgctgcg gatcataaga gatgtcggta ttggcaagaa
    22561 atccccacaa cgggaatgtg aagccacgat agttcatggc agcatcttcc acatcggcct
    22621 gtaaccattg ccgtgcatcg ccaaaatgtt cgccgacaat atacgcttcc ggctgggttt
    22681 ctttcgccgc ttcggtgatc ccggcaacgt gctgcatatt attgcgcgcc ccacccgcct
    22741 cccccagcat atgcaccaca tccagccgcc agccgtccat attccacggc gctttcagcc
    22801 agtggcggac aatactgtct tccccgcgat aaatttcatt caccagactt tccgactgat
    22861 aatccagctt cgtcaagctg gcatagccaa gccagtcgag cgccgtgcca tcatcactaa
    22921 acgagtacca gtcgcgccag ggcgattcag agttgtgaca agctcccccc gtgccaagat
    22981 tgtgcctgtc aaaccaggca tgggaatcgc cactgtggtt aaacacgccg tccagcacca
    23041 gccgcattcc cagctgctgc gtattgtgtc gcaaacgcag caacgcccca tcaccgccaa
    23101 actgcggatc gacatggcga taatcctcgg tatcgtattt atgtacgctg ggagctttaa
    23161 acaccggatt gagatacagc gctgtcacgc caagcttttt cagatacggc agtttttcgc
    23221 ttatcccgtc cagatcgccg ccatagaacg ttgatccgcc cgcctgcgcc gtgaccggtt
    23281 catcccagtc acgcaagatg atctcttgtc cggctgcatg atggtaatag acatgatcct
    23341 gttcagcttc acgaggaaga ctacgcgcaa aacgatcagg gaagatctga taaaaaatct
    23401 gatccgcagc ccattgtggg ccgatatcag gtacatcgac ggcaaactgc tccagtcgtg
    23461 ccggcggcat tcggctgaag ccctgcggtg taaaccaacg ctggcgatcg tgccacagca
    23521 gtttgaaact gtaacgccgc cggggttgtc cgctggagag atcaatcgcc gcacgccatg
    23581 cggtgacgcc tggctgcggc tgactgcgct gcttatgcat tggtactgac atttcttcgt
    23641 tatcgtgttc tgtacgcagc ataatgcgct gcggtgggtc ttcgcccgtc agccacagtg
    23701 taatgagcag ttgatctttg ctttgtttaa caaatggggg caccggcagg tgccatgcat
    23761 ttaacatcat aattcccctg tgatgaaaat ctgatcacct tgccatagcc taatgcagga
    23821 tcactcatca tctgaaggat atttggggga ggaggacggg ggcggataac tttcggaata
    23881 gccggatcag gatttcctcc tgaaccggct tacagaacgc aagtattatt ggttttcagc
    23941 cagctgacga tcgtggcggc gtttaaacat ccagccaatc aacagcacaa caatccacgc
    24001 gaaaccgaca tacagcgaga tacgcgtatc cgggtgataa ccaatcaacc cgataataaa
    24061 gagcaggaaa atcagcccgc cgatggtcgt tgctacccca cccggcactt taaatttcag
    24121 cgccttaact tcttctggcg gcaaacggcg acggaaggcg atttgcgaca gcaggatcat
    24181 aatccacacc cataccgtgg cgaaggttgc cagcgaagcg atcaccagga agacgttttc
    24241 cggcatgatg tagttcagat acaccgcaaa cagcagcgcg gtagtcatca ccagcaccgt
    24301 aacccacgga ataccgcgac gcgacgtttt gctgaaaatt ttcggtgcgc tgccctgctc
    24361 tgccataccg tggagcatac ggcctacgcc aaatacgtca ctgttaattg ccgacagcga
    24421 agcggtcagc acaacaaagt taagaatgct ggcggcaaag gtaatgccca tatgctggaa
    24481 cgtcagtacg aacgggctac cggcagtgcc aacctgattc cacgggtaga tagacataat
    24541 gacgaacagc gtaccaacgt agaacaccag aatacgcatc ggcacggagt taatcgcacg
    24601 cggaatcgat ttctcaggat ctttcgcttc accggcggta atcccgataa tttcgatccc
    24661 accgtaagca aacatcacca tttgcaacga cattaccatg ccaagccagc cgttactgaa
    24721 gaagccgccg ttgctccaca ggttatgaat accggtcggt tgcccgccgt tgccaatccc
    24781 ccagatgatg atgccgaaac cggcgacaat catgatgatg atggtggcga ctttaaagaa
    24841 cgagaaccag aattccagct caccgaagac cttcacgctc atcaggttta cggcgcaaat
    24901 gatcagcacc acgctcagta cccaaatcca gtgcggcacc gtcgggaaca agacacccat
    24961 atagatacca aaagcggtca catcggcgat ggcgacaata aggatttcaa aacagtaggt
    25021 ccatccggta atgtaacctg ccagcgggcc aaggttttcc tgcgcataac gcgagaaaga
    25081 gctggcggcc gggttatgta ccgacatttc ccccagcgca cgcataatga tatacgccgc
    25141 gataccaccg ataatatagg ccaacaacac gctcggaccg gccattttga tggcgtctgc
    25201 cgaaccgtaa aacagcccgg tgccaattgc tgaacccagt gccataaagc gtatgtggcg
    25261 ggtacttagc ccacgcttta gcttattctt actttccatc aattcctaac ccatcaacac
    25321 aataaaaaac cacggggcat cgcagccccg tggttaaaac aaatgttcag tgatttagtg
    25381 agcgctggag gtcacctgac gacctgccgc gcgatcccag ataatggcca gaaccaccat
    25441 caccactgtt ggcattaacc acgccagacc ttgttcggcc agcggtaaac gctgcgccca
    25501 ggacggtaag atatcgctga atgcagatgc tttgatcccg tcgagaatac caaaaagcag
    25561 gctgataaac atcggcggag caatcacgcg ggacgaatta tgccaccatg agcgtgtaaa
    25621 acttaataca accagtgcga tacacggcgg ataaatagcg gtcagcaccg gtacggagat
    25681 ctggatcagc tggcttaagc cgaggttaga caccaccatc gagaagccgc cgaggataaa
    25741 caccagcgta cgataagaga gcggtacgta ctgggcaaag aattctgcac aagcacaggt
    25801 caggccaacc gccgtgacca gacaggcgat gaagattaac gccgccagca ggaagctacc
    25861 gccgccgcca aaggtgtgct gaacgtaagc atgcagaata gcagcgccgt ttgcagactg
    25921 atcgaccagc gacgcgctgt ctgaacccag acagaacagc gccaggtaca gcaaagtcag
    25981 accaacaccc gccatcaggc cagcccagac ggtataacgg gtcagcagac gcgcttcggt
    26041 aacgccacga gaacgcgccg cgttaacaat aacgatacca aacaccattg cgcccagcgt
    26101 atccatggtc agatagccgt taacgaagcc gttagaaaat gcagcgtttt gataagcctc
    26161 agtcgccgtg ctgatagaac ccgccggcca gacaatcgcg acaacagaca ggatgaccag
    26221 cgcgataatt ttcagcggcg caaggaagtt gcccacggta tccagcagct tgcccggata
    26281 gagcgaaacc agaataacga tagcgaaata gaccaggctg tagataaaca gcggcagcgc
    26341 ggaatcaccc gtcagcggcg caatccccac ttcaaaggaa acggtagctg tacgcggcgt
    26401 agcgaaaagc ggccccaccg ccaggtaaca aaccgttgcc agcagtacgc cagcgacttt
    26461 accgattggc gtgctgaggc tgtcaacacc gccgccaact tttgccagcg ccactaccgt
    26521 taataccggc aggccaacgg cagtaatgag gaagccgaat gccgcagtcc agacgtgttc
    26581 gcctgcctgt aagccgacca ttggagggaa aataatgtta cctgcgccga cgaacaacgc
    26641 aaatgtcata aagcccagag cgatgatatc gcgcgatctt aattgatggg tcataaatca
    26701 tactgcctgt ggatgtggtg ttgtaacgtt aaatttttgt gccaatccct ggggatgata
    26761 cagcggtata tttaaacaat tttagcggga aatactccca accaggcgcg gcgaagaata
    26821 gtttttcgat ttaccgcgtt aatgcagtca gtatgacagc acctgaagcg caatttaaac
    26881 gcttataacg ttttaaagca aggagggatc taaaaaccag aggtatatag cgataagaaa
    26941 tcaccaacca gtataaaaca ctatttccgt gaaaaagaca tggctaatca tgcgaacaaa
    27001 aaatcaccaa ccgttgtaaa atctaacgcc cgtattttga ctgcaaagaa aataagccag
    27061 catcggctgg cttatggcaa taaaagatga caaaggcgga ttaatcgctg tttttggcaa
    27121 ttaaacgttc cgggataaca aaactgaaac gtgttccttt tcctactgta ctctcaatat
    27181 tcaggcgact ttcgtgatga ttcaccgcat gtttcacgat cgctaaccct aatccgctac
    27241 cgccggtttg ccgggaacgc gctttatcaa cgcgataaaa acgctcggtc aggcgcggaa
    27301 tatgctccgg tgcaatgccc ggtccgttat cttcaacgct aaattcggca ccgtgcggca
    27361 ctcgctgcca gcgtacggtg atatgcgtgc cttccggcgt atgattcacg gcgttataga
    27421 ccaggttcga aatcgcactg cgtagctgat cttcgttgcc agacaccttg aggccgttat
    27481 ctatctcaaa ggtaaatgtc tgttttttct gactcagagt ctgagcctcg cgctcaacaa
    27541 cgcgcagcat catcggcaca tcaacctttt cattgagcaa ttgcgtcggc gcggcttcaa
    27601 ttttcgacag cgttagcaac tgcttcacca gcccttccat ccgctgggtc tgctcgcgca
    27661 tggtgtgcaa cgctttttcg cgtaccgcgc cttccagcgg ctgctcatcc atcatctcca
    27721 ggtaaccctg taacacggtc aatggcgtac gtaactcatg gctcacgttg gcaaaaaagt
    27781 tacgccgcgc cccttccagt tgatgcattt gcgtgacatc acgcgccacc atcagcaact
    27841 gtttgtgggt ataaggcatg acgcgaattt ccagatgccg cccggtgttg agcaccagat
    27901 tgagcgggcg agaaaaatca cgcgttttca gatattgcgt aaactccggg taacgcagta
    27961 ggttaaggat gttctgcccg ttatcttccg gccagcgcaa accaagaatt tgttgcgcca
    28021 gaccgttaca ccagaaaata ccgccctctt ccgtggtcag caccaccgcg tcgggcagcg
    28081 actccgcgcc gctacgaaag cgtttaatca gattgcccag ttcacggcgg cgttttttat
    28141 ttcgcagctg catctggtgt aagccgtata gtagcggttc ccagctacca cgccccggtg
    28201 gcggggtcat actgcgatcc acccacagcc accatgaaag gcgcaataaa ttccagaaat
    28261 gccagataag cagtcctgtt accgatgcca gcaaaaacca gggcaggtaa ccaaaaaatg
    28321 cacccaggat gaaagccggg aggcagcaaa gtagcagctc cagcaccagc cttttccacg
    28381 acagccgttc cagcacgtaa gatactccag ttaagaaatc ataagccctg ctctgcgtcc
    28441 gatgagcaag gcgttaaaag cgggttgaaa aacgatatcc tgtaccgcgc acggtctgca
    28501 ccatgcggtc atgcccgccg ggctccagtg ctttacgcag gcgacgaatg tggacatcga
    28561 ccgtgcggtc ttccacatac acgttagttc cccagacgtg gtttaacagc tgctcgcggc
    28621 tgtacacgcg ctcaggatgc gtcataaaaa agtgcagcag tttaaattct gtcggcccca
    28681 tctccagcgg ctcttcgccc gccatcactc ggtgagatgt cgggttgaga cttaatccct
    28741 gcatcttaat cacctcttcc accgccattg gcgaaatacg gcgcattacc gctttgattc
    28801 gcgccaccag ctccttcggc gaaaatggct tggtgatata gtcatccgcg ccggtttcaa
    28861 ggccgcgcac gcgatcttct tcttcccctc tggcggtcaa catcaccact ggaatatccc
    28921 gggtcatcga ctcgcgcttg aggtgtttga tgaactggat accggagccg ccaggtaaca
    28981 tccagtcaag gagaattaaa tccggccagg gttcattcag ttgattcaca gcactgtcat
    29041 aatcttccgc ttcgaccggc tgaaagccat tttgttcgag cacgaagcag accatttcgc
    29101 gaattggagc ttcatcttct acgaccagaa tacgtctcgc catgatttgc cctgttgtaa
    29161 taaataggtt gcgatcatta atgcgacgtc attatgcgtc agatttatga cagatttatg
    29221 aaaagctcgt cgcacatatc ttcaggttat tgatttccgt ggcgcagaaa aaagcaaatg
    29281 gcacatctgt ttgggtataa tcgcgaccat gctttttcgc cagggaaccg ttatgcgcat
    29341 ccttcacacc tcagactggc atctcggcca gaacttctac agtaaaagcc gcgaagctga
    29401 acatcaggct tttcttgact ggctgctgga gacagcacaa acccatcagg tggatgcgat
    29461 tattgttgct ggtgatgttt tcgataccgg ctcgccgccc agttacgccc gcacgttata
    29521 caaccgtttt gttgtcaatt tacagcaaac tggctgtcat ctggtggtac tggcaggaaa
    29581 ccatgactcg gtcgccacgc tgaatgaatc gcgcgatatc atggcgttcc tcaatacaac
    29641 cgtggtcgcc agcgccggac atgcgccgca aatcttgcct cgtcgcgacg ggacgccagg
    29701 cgcagtgctg tgccccattc cgtttttacg cccgcgtgac attatttcca gccaggcggg
    29761 gcttaacggt attgaaaaac agcagcattt actggcagcg attaccgatt attaccaaca
    29821 acactatgcc gatgcctgca aactgcgcgg cgatcagcct ctgcccatca tcgccacggg
    29881 acatttaacg accgtgggtt ccagtaaaag tgacgccgtg cgtgacattt atattggcac
    29941 gctggacgcg tttccggcac aaaactttcc accagccgac tacatcgcgc tcgggcatat
    30001 tcaccgcgca cagattattg gcggcatgga acatgttcgc tattgcggtt cccccattcc
    30061 actgagtttt gatgaatgcg gtaagagtaa atatgtccat ctggtgacat tttcaaacgg
    30121 caaattagag agcgtggaaa acctgaacgt accggtaact caacccatgg cggtgctgaa
    30181 aggcgatctg gcgtcgatta ccgcacagct ggaacagtgg cgcgatgtat cgcaggagcc
    30241 acctgtctgg ctggatatcg aaatcactac tgatgagtat ctgcatgata ttcagcgcaa
    30301 aatccaggca ttaaccgaat cattgcctgt cgaagtattg ctggtacgtc ggagtcgtga
    30361 acagcgcgag cgtgtgttag ccagccaaca gcgtgaaacc ctcagcgaac tcagcgtcga
    30421 agaggtgttc aatcgccgtc tggcactgga agacctggat gaatcgcagc agcaacgtct
    30481 gcagcatctt ttcaccacga cgttgcgtac cctcgccgga gaacacgaag catgaaaatt
    30541 ctcagcctgc gcctgaaaaa cctgaactca ttaaaaggcg aatggaagat tgatttcacc
    30601 cgcgagccgt tcgccagcaa cgggctgttt gccattaccg gcccaaccgg tgcggggaaa
    30661 accaccctgc tggacgccat ttgtctggcg ctgtatcacg aaactccgcg tctctctaac
    30721 gtttcacaat cgcaaaatga tctcatgacc cgcgataccg ctgaatgtct ggcggaggtg
    30781 gagtttgaag tgaaaggtga agcgtatcgc gcgttctgga gccagaatcg ggcgcgtaac
    30841 cagccggacg gtaatttgca ggtgcctcgc gtagagctgg cgcgctgcgc cgacggcaaa
    30901 attctcgccg acaaagtgaa agataagctg gaactgacag cgacgttaac cgggctggat
    30961 tacgggcgct tcacccgttc gatgctgctt tcgcaggggc aatttgctgc cttcctgaat
    31021 gccaaaccca aagaacgcgc ggaattgctc gaggagttaa ccggcactga aatctacggg
    31081 caaatctcgg cgatggtttt tgagcagcac aaatcggccc gcactgagct ggagaagctg
    31141 caagcgcagg ccagcggcgt cacgttgctc acgccggaac aagtgcaatc gctgacagcg
    31201 agtttgcagg tacttactga cgaagaaaaa cagttaatta ccgcgcagca gcaagaacaa
    31261 caatcgctaa actggttaac gcgtcaggac gaattgcagc aagaagccag ccgccgtcaa
    31321 caggccttgc aacaggcgtt agccgaagaa gaaaaagcgc aacctcaact ggcggcgctt
    31381 agtctggcac aaccggcacg aaatcttcgt ccacactggg aacgcatcgc agaacacagc
    31441 gcggcgctgg cgcatattcg ccagcagatt gaagaagtaa atactcgctt acagagcaca
    31501 atggcgcttc gcgcgagcat tcgccaccac gcggcgaagc agtcagcaga attacagcag
    31561 cagcaacaaa gcctgaatac ctggttacag gaacacgacc gcttccgtca gtggaacaac
    31621 gaactggcgg gttggcgtgc gcagttctcc caacaaacca gcgatcgcga gcatctgcgg
    31681 caatggcagc aacagttaac ccatgctgag caaaaactta atgcgcttgc ggcgatcacg
    31741 ttgacgttaa ccgccgatga agttgctacc gccctggcgc aacatgccga gcaacgccca
    31801 ctgcgtcagc gcctggtcgc gctgcatggg cagattgttc cccaacaaaa acgtctggcg
    31861 cagttaatgg tcactatcca gaatgtcact ctggaacaga cgcaacgtaa tgtcgcactt
    31921 aacgaaatgc gccagcgtta taaagaaaag acgcagcaac ttgccgatgt gaaaaccatt
    31981 tgcgagcagg aagcgcgcat caaaacgctg gaagcccagc gtgcacagtt acaggcgggt
    32041 cagccttgtc cactttgtgg ttccaccagc cacccggcgg tcgaggcgta tcaggcgctg
    32101 gagcctggtg ttaatcagtc tcgattactg gcgctggaaa acgaagttaa aaagctcggc
    32161 gaagaaggtg cggcgctgcg cgggcaactg gatgcattaa cgaagcagct tcagcgcgat
    32221 gaaaacgaag cgcaaagcct ccgacaagat gagcaagcac ttactcaaca atggcaagcc
    32281 gtcacggcca gcctcaatat caccttgcag ccacaggacg atattcaacc gtggctggat
    32341 gcacaagatg agcacgaacg ccagctgcgg ttactcagcc aacgacatga attacaaggg
    32401 cagattgccg cgcataatca gcaaattatc cagtatcaac agcaaattga acaacgtcag
    32461 cagcagctct taacggcatt ggcgggttat gcactgacat tgccacagga agatgaagaa
    32521 gagagctggt tggcgacacg tcagcaagaa gcgcagagct ggcagcaacg ccagaacgaa
    32581 ttaaccgcgc tgcaaaaccg tattcagcag ctgacaccga ttctggaaac gttgccgcaa
    32641 agtgatgatc tcccgcacag cgaagaaact gtggcgctgg ataactggcg gcaggttcat
    32701 gaacaatgtc tcgcattaca cagccagcag cagacgttac agcaacagga tgttctggcg
    32761 gcgcaaagtc tgcaaaaagc ccaagcgcag tttgacaccg cgctacaggc cagcgtcttt
    32821 gacgatcagc aggcgttcct tgcggcgcta atggatgaac aaacactaac gcagctggaa
    32881 cagctcaagc agaatctgga aaaccagcgc cgtcaggcgc aaacgctggt cactcagaca
    32941 gcagaaacgc tggcacagca tcaacaacac cgacctgacg ggttggctct cactgtgacg
    33001 gtgtagcaga ttcagcaaga gttagcgcaa actcaccaaa agttgcgtga aaacaccacg
    33061 agtcaaggcg agattcgcca gcagctgaag caggatgcag ataaccgtca gcaacaacaa
    33121 accttaatgc agcaaattgc tcaaatgacg cagcaggttg aggactgggg atatctgaat
    33181 tcgctaatag gttccaaaga gggcgataaa ttccgcaagt ttgcccaggg gctgacgctg
    33241 gataatttag tccatctcgc taatcagcaa cttacccggc tgcacgtgcg ctatctgtta
    33301 cagcgcaaag ccagcgaggc gctggaagtc gaggttgttg atacctggca ggcagatgcg
    33361 gtacgcgata cccgtaccct ttccggcggc gaaagtttcc tcgttagtct ggcgctggcg
    33421 ctggcgcttt cggatctggt cagccataaa acacgtattg actcgctgtt ccttgatgaa
    33481 ggttttggca cgctggatag cgaaacgctg gataccgccc ttgatgcgct ggatgccctg
    33541 aacgccagtg gcaaaaccat cggtgtgatt agccacgtag aagcgatgaa agagcgtatt
    33601 ccggtgcaga tcaaagtgaa aaagatcaac ggcctgggct acagcaaact ggaaagtacg
    33661 tttgcagtga aataactatt cagcaggata atgaatacag aggggtgaat tatctcttgg
    33721 ccttgctggt cgttatcctg caagctatca ctttattggc tacggtgatt ggtagccgtt
    33781 ctggtggttg tgatggtggt atgaaaaaag tcattttatc tttggctctg ggcacgtttg
    33841 gtttggggat ggccgaattt ggcattatga gcgtgctcac ggagctggcg cataacgtag
    33901 gaatttcgat tcctgctgcc gggcatatga tctcgtatta tgcactggtg gtggtggtcg
    33961 gtgcgccaat catcgcactc ttttccagcc gctactcact caaacatatc ttgttgtttc
    34021 tggtggcgtt gtgcgtcatt ggcaacgcca tgttcacgct ctcttcgtct tacctgatgc
    34081 tcgccattgg tcggctggta tccggctttc cgcatggcgc attttttggc gtcggagcga
    34141 tcgtgttatc aaaaattatc aaacccggaa aagtcaccgc cgccgtggcg gggatggttt
    34201 ccgggatgac agtcgccaat ttgctgggca ttccgctggg aacgtattta agtcaggaat
    34261 gctggcgtta caccttttta ttgatcgctg tttttaatat tgcggtgatg gcatcggtct
    34321 atttttgggt gccagatatt cgcgacgagg cgaaaggaaa tctgcgcgaa caatttcact
    34381 ttttgcgcag cccggccccg tggttaattt tcgccgccac gatgtttggc aacgcaggtg
    34441 tgtttgcctg gttcagctac gtaaagccat acatgatgtt tatttccggt ttttcggaaa
    34501 cggcgatgac ctttattatg atgttagttg ggctagggat ggtgctggga aatatgctaa
    34561 gtggcaggat ttcaggacgt tattcaccac tgcgcattgc agcagtgact gactttatca
    34621 ttgtactggc actgctgatg ctctttttct gcggcggcat gaaaacaacg tcgcttattt
    34681 ttgcttttat ttgttgcgcg ggattatttg ccctttcagc acctctgcaa atattgttac
    34741 tgcaaaatgc caaaggagga gagttattag gtgccgcagg tgggcaaata gcgtttaacc
    34801 tcggtagcgc cgtcggcgca tattgcggag gtatgatgct gacgctgggg ctggcatata
    34861 attacgtggc gctgcctgcc gccctgcttt cgtttgctgc gatgtcatcg ttgctgctgt
    34921 atggtcgcta taagcgccag caagcggcgg atagtccggt gctggcgaaa ccactggggt
    34981 agattactgt cacggtgcgc gttgcttatt gccggatgcg gcgtgaacgc cttatccgcc
    35041 ctacgcggtt ctggcacatt ttgtaggcct gataagacgc ggcaagcgtc gcatcaggca
    35101 tgatgcgcca attgcctacg ttttttactc ttgtggccat aaccacgcag cgccgcgtac
    35161 gccgctggaa tcaccgtgct tcgccttact caccggcgtt tcacattcgc cgccgaagac
    35221 aaattgttta atcaactgcc caaccgtttg atataaacgg tctacattgc tcatcccgcc
    35281 ccccaggaca atcacatccg gatcgagaat attcacgaca tgtgccagcg attttgccag
    35341 ccgcagctcg tagcgacgca atgccagttc cgctaccgga tcgctttctt caaccaggcg
    35401 gataatttca ctgcctttca gcgcatgtcc gctcaaacga cgataatccg tcgcgaatcc
    35461 cgtgcccgaa ataaaggttt caatacaacc ttgtttaccg caataacaag ggacttcctc
    35521 gcgataacgc agttcgtctt cgtccatcca cggtagcgga ttgtgtcccc actcacctgc
    35581 cgtgccattg ccgccgatat gcgtccgccc attgaatgcc acgcccgcgc cgcatcccgt
    35641 gccgataatc acggcaaata ccgtctgcgc tcccgctgcc gcgccatcta ctgcttctga
    35701 aaccgccaga cagttggcat catttgccag ccgcacttcc cgctgcaacc tcgcgcttaa
    35761 gtctttatcg aatggctgac cgttgagcca ggttgaattg acattcttca ccacaccggt
    35821 gtagggcgaa atcgaacctg gaatgcccat acctaccgtt ccgcgctgcc ctgtcgcctg
    35881 ctccgccata tcaaccaacg tggcgatcgt ttcaatagtc tgccggtaat catcacgcgg
    35941 cgtgggcaga cgatggcggt acaactgctc ccctgcatcg cccagtgcaa tcacttcagt
    36001 tttggtgccg cctaaatcga tacctatacg cactgtactc tccttatttt tttcaatatc
    36061 aatagcgtag agacggacaa ccggattggc aatgcaaggc cgccgacaat tcgttatcat
    36121 gcccgctaaa tttaacgaca aggccgtgga aattatcatg ctgtggttca aaaatttaat
    36181 ggtttaccgt cttagccgcg agatttcgct gcgtgcagaa gagatggaaa aacagctagc
    36241 ctcgatggca tttaccccat gcggcagcca ggacatggcg aagatgggct gggttcctcc
    36301 gatgggatcg cacagcgatg cgttaacgca cgttgccaat ggtcaaattg ttatctgcgc
    36361 gcgcaaagaa gaaaaaatcc tcccgtctcc ggtgattaaa caggcgctgg aagcgaaaat
    36421 cgccaaactg gaagcggaac aggcgcgtaa gctgaagaaa accgaaaaag attcgctgaa
    36481 agacgaagtg ctgcactctc tgctgccgcg tgctttcagc cgttttagcc agacaatgat
    36541 gtggctcgac acggttaacg gtttgattat ggtggactgc gccagtgcca aaaaagcgga
    36601 agatacgctg gcattactgc gtaaaagcct ggggtcgtta ccggttgtac cattgagcat
    36661 ggaaaacccg attgaactga cgctgaccga atgggttcgc tccggtagtg cggcacaggg
    36721 cttccagctg cttgatgaag ccgagctgaa atcgttgctg gaagatggcg gcgtgatccg
    36781 cgcgaagaaa caagatctga ccagcgaaga gatcaccaat cacattgaag ccggaaaagt
    36841 ggtgactaaa ctggcgctcg actggcagca gcgcattcag tttgtgatgt gcgacgatgg
    36901 ttcgctcaag cgtcttaagt tctgcgacga gctgcgcgat caaaacgaag atatcgaccg
    36961 tgaagatttc gcccagcgtt ttgatgccga tttcatcctg atgactggtg aactggcagc
    37021 gttaattcaa aacctgattg aaggattagg tggcgaagca caacgttaat tgctgatttt
    37081 cctttaatgc cggatgcgac gcctgccgcg tcttatccgg cgtacgaagc cacaccaggc
    37141 atataattat tcgctacggc gagcaataat ttttagcgca gcaatattat gcgttttacg
    37201 ccgtaactga tctaatcgcc tcatccctat tcgcctgtca gcagcacgca gaatattcac
    37261 taacccccat ttatcgctgg taaattgttt tgttaacagc tccgcttttg gggtatcaat
    37321 atattcacgc aataaatcag aaattgaaca aatatccgtc aggtaaggat accagcaagt
    37381 ttcaccgtga aaattacgca ctccagcgtt acctgcaata aagtctttcg ggtaatccca
    37441 gatcacgttt ttatccaacg tgatccaata acgcggtaaa tcggtacttc caccattttg
    37501 gctgcgcatg ggataacggg tacagtgaat ttgcaggttg atcgttggcg tcagtaagtc
    37561 atagatctcc cgctgtaact tgctccatgg acgttgtgtc attgtttttc ctcaagccgg
    37621 tgacgcgctt cactgcgaat tttccgcgtt gccccggttt tcactaaatc acgtaactgc
    37681 gaaagatcgg gttgttcgac aaataagagt aatgatggat cctcttttaa ccacgccgtt
    37741 ttcacatcgg cagccagctg tggattatta atagccagcg accgatcctg aggctcagtc
    37801 aatgtcgtta acaacgacgc cggggtatgt gcgttgcgta atgccgtttg gcgcaaatga
    37861 tcattgcgac tttgcagcaa tgcacgccac cattcatcat ctgatttatc ttttatcgta
    37921 gccagttccg caaaaaatac ctggagatct tcactgatat ttatatcaac cttgatgaat
    37981 tatgaatata tcggaaattg aaaaaatcgc agcaaaggaa atattattat gggttaaaga
    38041 gaggatattt cagaaaaatg ccctcccgtt caaggggaag gcgaggaatt acagatagcg
    38101 gcacagataa gaggtaggtt cggcaacttg aagatgaaac tcactgtgac cgggaacatt
    38161 aaacaccgaa ccggcttcat acacttgcca gtcggtcgcg tcaggcagta acacattcag
    38221 cgcgccactg attaccgtca tctcttccgg ctcagcggtg ctgaaggtgt attcgccttc
    38281 aaccataaca cccacgctgg cgcgaccagt gctgctgctg gaaaagccga tcgatttcac
    38341 tttgccggaa aagtactcat tactttgaag cataaactgg ccctgtctgg tagttgaaca
    38401 tctacttcac tataggggcc agaggcgctg gctgtcacgc aaaattacac cagtaattcc
    38461 gcagccagcc gtgcaatcaa tacgttagag agcaagacag gaacatcgag ctgtttttgc
    38521 agtaaatcgc gatgacgttg gtgaaatcct aaacaatcca gcatgatgac atcagcccct
    38581 tttgccagta attctttccc agcatcaatg attttttgtt ctgaatcatg aatggggtta
    38641 cccaatgaaa ataccggcga tttctgcaaa atttgccatt tttgcgcctg aacgggcagc
    38701 atctcctcaa ccggaacgat aacccccacc tgatgatctt caacaataga ggaaaccagt
    38761 ggaggcaata ttcgcgacgg ctcaagaaag atcgtattac gcgcagtcat actactaatg
    38821 tttgctgtac tcattaataa aatgacgtca taaccctgat tatcgagcac ttcaaccaca
    38881 ccttgcagat ctcgctccac tttacgacgc gaaacatggg ccagctggtt gtcatttaat
    38941 aatgtgagaa tggtgtcttc gcctgcttct ggcgcgtact ccgccatcac ttcttcacga
    39001 cttaacttcc ccagcaggct atgatgggaa atattatctt cgtcaatgta ttccgtcagg
    39061 agcggcaaaa cctcctgcat aggtacaatg ccgatggtca ggatcgccaa cgacgcactc
    39121 attgttcatc caccttactt ttctttcgtc gttaattacc gggcaagtgt gaagcaccac
    39181 tgctgacact tacttgtgct acaaatgaca aaaagcgtag cagcagacgt gcacggcata
    39241 atgtaaagat tccaaatgat tccagtaatg gaatgttatt gtttaatatt ctaattatta
    39301 gaaaaacata aattatgaaa aatgtgacgc agatcccttg tagtggcact cagaatccct
    39361 tccgagcagt ctgatgcggt cactctttgt cagggtcctc atagcgtttt ttcgcatcca
    39421 tcgcttcctg agccgtcgga tgttcactga tcaacgagtc tggtttagga tgatcggctc
    39481 tgagttggta ccaggttacc gtctgtcctg gctttccttt ttcaatcgtc actatatatg
    39541 cttcacgagg ataaggcggt ttcgttggca tcgttcttcc ttatttcacg ggatgaacgt
    39601 taagtatagg cgctctaaaa tcaacaattg atcgtctgtg ccagggcgct acgaatttca
    39661 gaaatcacct ggctgggttc gtttgttgcg tcgatgataa tatgcgcaac ttcgcgatat
    39721 agcgcatcgc gttcttccag cacttcctga acttcttcgc tcagcggttt tcccgttaag
    39781 gttggccgta aatcttcttc cggtgcagct tgcagtcggt taaccaggac tgatactggc
    39841 gcacacaaat aaaccacgat cccgttattt tgcatgaagt gacgattaaa ttccgtcaga
    39901 ataatgccgc cgcctgtagc gataacggtg gatgccgcag ttaccgcttc cagcgccgcc
    39961 gtttctctgg cgcgaaatcc cgcccactct tccctttcga cgatctccgc gaccgtcata
    40021 ttgagctgtg attgcaacca ctgatcggta tcgacaaacc gacggttaag cgaatcggca
    40081 agggccattc cgaccgttgt tttaccacag ccccgaggcc cgatcagaaa aagaggttgt
    40141 gtcatcgtgg gttttcccca ataggtcgca atgcggcgaa agccggtgtc atgagaatag
    40201 cgatcatacc atcaaactag tacaatttcg attgtaaaga aaaaattcca cttaaagtaa
    40261 aaatctcaat acatccctta ctataccaat aaatattcaa gaatgaagtg taaataataa
    40321 attacattta gccacgacta cgttgcactt ccagccacca cttctccagc tccgcggcaa
    40381 aggcctggcg gtcacgttgt gaaaggctat ctggtccgcc ggtctggatc ccactggcac
    40441 gtaaggtatc cataaaatcg cgcatcgtca ggcgctcacg aatggtcgct ggcgtgtaac
    40501 gttcgccgcg cgaattaagc gccgctgcgc ctttctcgat ggcttcagca gccaaaggta
    40561 tatctgcggt gatcaccaaa tcgcccgctt cacactgccg gacaatttcg ttatcggcaa
    40621 catcgaaacc tgccgcgacg cgcagcgtac gaataaatcg cgatggcggc acgcgtaaac
    40681 tctggtttgc taccagtacc agcggcatct gcatacgttc cgccgcgcga tacaaaatct
    40741 ctttaattac attgggacac gcgtcggcat ccacccaaat tgtcataaag tcatcctttg
    40801 ttaggtaatc ctctattgtg tcgcgctttt gccttccggc atagttctgt ttatgcttct
    40861 gccagcgatt atcaaaacaa tgaatttcac ggcaggagtg aggcaatgga aaagaaaatc
    40921 ggttttattg gctgcggcaa tatgggaaaa gccattctcg gcggtctgat tgccagcggt
    40981 caggtgcttc cagggcaaat ctgggtatac accccctccc cggataaagt cgccgccctg
    41041 catgaccagt tcggcatcaa cgccgcagaa tcggcgcaag aagtggcgca aatcgccgac
    41101 atcatttttg ctgccgttaa acctggcatc atgattaaag tgcttagcga aatcacctcc
    41161 agcctgaata aagactctct ggtcgtttct attgctgcag gtgtcacgct cgaccagctt
    41221 gcccgcgcgc tgggccatga ccggaaaatt atccgcgcca tgccgaacac tcccgcgctg
    41281 gttaatgccg ggatgacctc cgtaacgcca aacgcgctgg taaccccaga agataccgct
    41341 gatgtgctga atattttccg ctgctttggc gaagcggaag taattgctga gccgatgatc
    41401 cacccggtgg tcggtgtgag cggttcttcg ccagcctacg tatttatgtt tatcgaagcg
    41461 atggccgacg ccgccgtgct gggcgggatg ccacgcgccc aggcgtataa atttgccgct
    41521 caggcggtaa tgggttccgc aaaaatggtg ctggaaacgg gagaacatcc gggggcactg
    41581 aaagatatgg tctgctcacc gggaggcacc accattgaag cggtacgcgt actggaagag
    41641 aaaggcttcc gtgccgcagt gatcgaagcg atgacgaagt gtatggaaaa atcagaaaaa
    41701 ctcagcaaat cctgatgact ttcgccggac gtcaggccgc cacttcggtg cggttacgtc
    41761 cggctttctt tgctttgtaa agcgccaaat ctgccgattt caaccactca cgatagtgac
    41821 tcatttgtgg gttcagcggc gcaaccccca cactaatccg taaagttacc tgtggcgtat
    41881 tcggcaagcg taacgtgttt agcccttcac gcacccgtaa catggcggta atggcgctct
    41941 cagctggcgt accggacatg attactgcaa actcatcgcc gccaaaccga ccaatcacat
    42001 cgctaccgcg cagggtaatt tgtaactgtc gggtaagcgc cacaatcgct tcatcgccca
    42061 catcatgacc ccaggtatcg ttgatgctct tgaaatggtc gatatcgata atcagtaacg
    42121 ttgcatcgcg attatgccgc cgacagttat caaattcatt gcgtaacata gtttcccaat
    42181 gacgtcggtt atacacgccc gtcatgccgt cgcgagtact catgacctgc aacctgcgtt
    42241 tatgttccgc cagtttggtt gccgtctggt agctgaccca gccaaacatc agaggataaa
    42301 cgacaataat gggaagggag agccaccatt ccagcggagc actattgaac gacacggtaa
    42361 tgcccgtaag ctcgagggtg acaaggcagg aaaccaccat caacaccaga cccgcgacaa
    42421 acagacgggg gccgcctgcc cccatcaaat tcagacacag aatcatcaac atagcggtgg
    42481 aaggcagcac gtttacgccc attacgccta cccacattcc cgctaatact gcatcggttt
    42541 ttaagttgta aatttcccgg ctaagcggat cgacggccct gctcgctatc tgccaggcta
    42601 aatgcggcca gacgaacgcc cagccgacca acaccagcca ccaccagccc ggcggcgggt
    42661 gcgaaaccag cgttgaagca atcggtaaga acatgccagc caggccaacc gcacggggta
    42721 gtctgacgcg acgggcgaag cgcagcccgg accgctgatg ttcgttttga ggagttaaag
    42781 gaggttcctc cccatgcgcc gccgcttttt tgaaaaagtt ttcatcattc attatttttg
    42841 ggaacattca gaaacaattt tcccaaatta tagagacggg gctaaaggca gaagtatgaa
    42901 atttcgggtg agccactttc gtagctcacc cgttagcact
//
LOCUS       DAZPAB010000014        40371 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZPAB010000014 DAZPAB010000000
VERSION     DAZPAB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.014"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            324..410
                     /gene="leuL"
                     /locus_tag="ACHUPG_000684"
     CDS             324..410
                     /gene="leuL"
                     /locus_tag="ACHUPG_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR0860416.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            503..2074
                     /gene="leuA"
                     /locus_tag="ACHUPG_000685"
     CDS             503..2074
                     /gene="leuA"
                     /locus_tag="ACHUPG_000685"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR0860417.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            2074..3165
                     /gene="leuB"
                     /locus_tag="ACHUPG_000686"
     CDS             2074..3165
                     /gene="leuB"
                     /locus_tag="ACHUPG_000686"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR0860418.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3168..4568
                     /gene="leuC"
                     /locus_tag="ACHUPG_000687"
     CDS             3168..4568
                     /gene="leuC"
                     /locus_tag="ACHUPG_000687"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR0860419.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4579..5184
                     /gene="leuD"
                     /locus_tag="ACHUPG_000688"
     CDS             4579..5184
                     /gene="leuD"
                     /locus_tag="ACHUPG_000688"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR0860420.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(5233..6411)
                     /gene="setA"
                     /locus_tag="ACHUPG_000689"
     CDS             complement(5233..6411)
                     /gene="setA"
                     /locus_tag="ACHUPG_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR0860421.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(6513..6644)
                     /gene="sgrT"
                     /locus_tag="ACHUPG_000690"
     CDS             complement(6513..6644)
                     /gene="sgrT"
                     /locus_tag="ACHUPG_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR0860422.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6733..8388
                     /gene="sgrR"
                     /locus_tag="ACHUPG_000691"
     CDS             6733..8388
                     /gene="sgrR"
                     /locus_tag="ACHUPG_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR0860423.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8422..8521
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8552..9535
                     /gene="thiB"
                     /locus_tag="ACHUPG_000692"
     CDS             8552..9535
                     /gene="thiB"
                     /locus_tag="ACHUPG_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR0860424.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9511..11121
                     /gene="thiP"
                     /locus_tag="ACHUPG_000693"
     CDS             9511..11121
                     /gene="thiP"
                     /locus_tag="ACHUPG_000693"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR0860425.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11105..11803
                     /gene="thiQ"
                     /locus_tag="ACHUPG_000694"
     CDS             11105..11803
                     /gene="thiQ"
                     /locus_tag="ACHUPG_000694"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR0860426.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(11917..12681)
                     /locus_tag="ACHUPG_000695"
     CDS             complement(11917..12681)
                     /locus_tag="ACHUPG_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0860427.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12767..13645)
                     /gene="araC"
                     /locus_tag="ACHUPG_000696"
     CDS             complement(12767..13645)
                     /gene="araC"
                     /locus_tag="ACHUPG_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR0860428.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13984..15684
                     /gene="araB"
                     /locus_tag="ACHUPG_000697"
     CDS             13984..15684
                     /gene="araB"
                     /locus_tag="ACHUPG_000697"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR0860429.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15695..17197
                     /gene="araA"
                     /locus_tag="ACHUPG_000698"
     CDS             15695..17197
                     /gene="araA"
                     /locus_tag="ACHUPG_000698"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR0860430.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17397..18092
                     /gene="araD"
                     /locus_tag="ACHUPG_000699"
     CDS             17397..18092
                     /gene="araD"
                     /locus_tag="ACHUPG_000699"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR0860431.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLHKH
                     YLRKHGAKAYYGQ"
     gene            18167..20518
                     /gene="polB"
                     /locus_tag="ACHUPG_000700"
     CDS             18167..20518
                     /gene="polB"
                     /locus_tag="ACHUPG_000700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR0860432.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20683..23589
                     /gene="rapA"
                     /locus_tag="ACHUPG_000701"
     CDS             20683..23589
                     /gene="rapA"
                     /locus_tag="ACHUPG_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR0860433.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23601..24260
                     /gene="rluA"
                     /locus_tag="ACHUPG_000702"
     CDS             23601..24260
                     /gene="rluA"
                     /locus_tag="ACHUPG_000702"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR0860434.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24377..25192)
                     /gene="djlA"
                     /locus_tag="ACHUPG_000703"
     CDS             complement(24377..25192)
                     /gene="djlA"
                     /locus_tag="ACHUPG_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR0860435.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25447..27801
                     /gene="lptD"
                     /locus_tag="ACHUPG_000704"
     CDS             25447..27801
                     /gene="lptD"
                     /locus_tag="ACHUPG_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR0860436.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27854..29140
                     /gene="surA"
                     /locus_tag="ACHUPG_000705"
     CDS             27854..29140
                     /gene="surA"
                     /locus_tag="ACHUPG_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR0860437.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29140..30129
                     /gene="pdxA"
                     /locus_tag="ACHUPG_000706"
     CDS             29140..30129
                     /gene="pdxA"
                     /locus_tag="ACHUPG_000706"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR0860438.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30126..30947
                     /gene="rsmA"
                     /locus_tag="ACHUPG_000707"
     CDS             30126..30947
                     /gene="rsmA"
                     /locus_tag="ACHUPG_000707"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR0860439.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30950..31327
                     /gene="apaG"
                     /locus_tag="ACHUPG_000708"
     CDS             30950..31327
                     /gene="apaG"
                     /locus_tag="ACHUPG_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR0860440.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31334..32176
                     /gene="apaH"
                     /locus_tag="ACHUPG_000709"
     CDS             31334..32176
                     /gene="apaH"
                     /locus_tag="ACHUPG_000709"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR0860441.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32254..32733)
                     /gene="folA"
                     /locus_tag="ACHUPG_000710"
     CDS             complement(32254..32733)
                     /gene="folA"
                     /locus_tag="ACHUPG_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR0860442.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32924..34786)
                     /gene="kefC"
                     /locus_tag="ACHUPG_000711"
     CDS             complement(32924..34786)
                     /gene="kefC"
                     /locus_tag="ACHUPG_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR0860443.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWVKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34779..35309)
                     /gene="kefF"
                     /locus_tag="ACHUPG_000712"
     CDS             complement(34779..35309)
                     /gene="kefF"
                     /locus_tag="ACHUPG_000712"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR0860444.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35357..36751)
                     /locus_tag="ACHUPG_000713"
     CDS             complement(35357..36751)
                     /locus_tag="ACHUPG_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0860445.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(36810..37097)
                     /gene="fixX"
                     /locus_tag="ACHUPG_000714"
     CDS             complement(36810..37097)
                     /gene="fixX"
                     /locus_tag="ACHUPG_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR0860446.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37094..38380)
                     /locus_tag="ACHUPG_000715"
     CDS             complement(37094..38380)
                     /locus_tag="ACHUPG_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0860447.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38431..39372)
                     /locus_tag="ACHUPG_000716"
                     /pseudo
     CDS             complement(38431..39372)
                     /locus_tag="ACHUPG_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(39387..40157)
                     /locus_tag="ACHUPG_000717"
     CDS             complement(39387..40157)
                     /locus_tag="ACHUPG_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR0860448.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         9735 a        11146 c        10579 g         8911 t
ORIGIN      
        1 attcaccaaa tcaatatcaa aaaaaatcgc aaaacatata attcaataca aatcatcagg
       61 ataggttttg caacgcgtgc attttgtccc ctttttcctc gttgattaga tgcaaaaatt
      121 tatgctgaaa tatgtcaacc gatgaaaagc gtcggtagtt aagcagaaat taatatcgct
      181 tactttaacc accgtagcac aattagctaa ttttacggat gcagaactca cgctggcggg
      241 acgtttttat tgcgtcaggg ttgacatccg tttttgtatc cagtaactct aaaagcatat
      301 cgcattcatc tggagctgat ttaatgactc acatcgttcg ctttatcggt ctactactac
      361 taaacgcatc ttctttgcgc ggtagacgag tgagcggcat ccagcattaa gccagcacgc
      421 agtcaaacaa aaaacccgcg ccattgcgcg ggttttttta tgcccgaagc gaggcgctct
      481 aaaagagaca aggacccaaa ccatgagcca gcaagtcatt attttcgata ccacattgcg
      541 cgacggtgaa caggcgttac aggcaagctt gagtgtgaaa gaaaaactgc aaattgcgct
      601 ggcccttgag cgtatgggtg ttgacgtgat ggaagtcggt ttccccgtct cttcgccggg
      661 cgattttgaa tcggtgcaaa ccatcgcccg ccaggttaaa aacagccgcg tatgtgcgtt
      721 agctcgctgc gtggaaaaag atatcgacgt ggcggccgaa tccctgaaag tcgccgaagc
      781 cttccgtatt cataccttta ttgctacctc cccaatgcac atcgccacca agctgcgcag
      841 caccctagac gaggtgatcg aacgcgctat ctatatggtg aaacgcgccc gtaattacac
      901 cgatgatgtt gaattttctt gcgaagatgc cgggcgtaca cccattgccg atctggcgcg
      961 agtggtcgaa gcggcgatta atgccggtgc caccaccatc aacattccgg acaccgtggg
     1021 ctacaccatg ccgtttgagt tcgccggaat catcagcggc ctgtatgaac gcgtacctaa
     1081 catcgacaaa gccattattt ccgtacatac ccacgacgat ttaggcctgg cggtcggcaa
     1141 ctcactggcg gcggtacatg ccggtgcacg ccaggtggaa ggtgcaatga acgggatcgg
     1201 cgagcgtgcc ggtaactgtt cgctggaaga agtcatcatg gcgattaaag ttcgtaagga
     1261 tattctcaac gtccacaccg ccattaatca ccaagagata tggcgcacca gccagttagt
     1321 tagccagatt tgtaatatgc cgatcccggc aaacaaagcc attgttggca gcggcgcatt
     1381 cgctcactcc tccggtatcc accaggatgg cgtgctgaaa aaccgcgaaa actacgaaat
     1441 catgacacca gaatctattg gtctgaacca aatccagctg aatctgacct ctcgttcggg
     1501 gcgtgcggcg gtgaaacatc gcatggatga gatggggtat aaagaaagtg aatataattt
     1561 agacaatttg tacgatgctt tcctgaagct ggcggacaaa aaaggtcagg tgtttgatta
     1621 cgatctggag gcgctggcct tcatcggtaa gcagcaagaa gagccggagc atttccgtct
     1681 ggattacttc agcgtgcagt ctggctctaa cgatatcgcc accgccgccg tcaaactggc
     1741 ctgtggcgaa gaagtcaaag cagaagccgc caacggtaac ggtccggtcg atgccgtcta
     1801 tcaggcaatt aaccgcatca ctgactataa cgtcgaactg gtgaaataca gcctgaccgc
     1861 caaaggtcac ggtaaagatg cgctgggtca ggtggatatt gtcaccaact acaacggtcg
     1921 ccgcttccac ggcgtcggcc tggccaccga tattgtcgag tcctccgcca aagccatggt
     1981 gcacgtactg aacaatatct ggcgtgccgc agaagtcgaa aaagagttgc aacgcaaaac
     2041 tcaacacaac gaaaacaaca aggaaaccgt gtgatgtcga agaattacca tattgccgta
     2101 ttgccgggag acggtattgg tccggaagtg atgacccagg cgctgaaagt gctgaatgcc
     2161 gtgcgcaacc gctttgcgat gcgcatcacc accagccatt acgatgtagg cggcgcagcc
     2221 attgataacc acgggcaacc actgccgcct gcgacggttg aaggttgtga gcaagccgat
     2281 gccgtgctgt ttggctcggt aggcggcccg aagtgggaac atttaccacc agaccagcaa
     2341 ccagaacgcg gcgcgctgct gccgctgcgt aagcacttca aattattcag caacctgcgc
     2401 ccggcaaaac tgtatcaggg gctggaagca ttctgtccgc tgcgtgcaga cattgccgct
     2461 aacggcttcg acatccagtg cgtgcgcgaa ctgaccggcg gcatctattt cggtcagcca
     2521 aaaggccgcg aaggtagcgg acaatatgaa aaagcgtttg ataccgaggt gtatcaccgt
     2581 tttgagatcg agcgtattgc ccgcatcgcg tttgaatctg ctcgtaaacg tcgccacaaa
     2641 gtcacctcaa tcgacaaagc taacgtgctg caatcctcta ttttatggcg tgaaatcgtc
     2701 aacgagatcg ccacggaata cccggatgtc gaactggcgc atatgtacat cgacaacgcc
     2761 accatgcagc tgattaaaga tccatcacag tttgacgttc tgctgtgctc caacctgttt
     2821 ggcgacattc tgtctgacga gtgcgcaatg atcactggct cgatgggcat gttgccttcc
     2881 gccagcctga acgagcaagg ttttggactg tatgaaccgg cgggcggctc ggcaccagat
     2941 atcgcaggca aaaacatcgc caacccgatt gcacaaatcc tgtcgctggc actgctgctg
     3001 cgttacagcc tggatgccga tgatgcggct tccgccattg aacgcgccat taaccgtgca
     3061 ttagaagaag gcattcgcac cggggattta gcccgtggcg ctgccgccgt tagtaccgat
     3121 gaaatgggcg atatcattgc ccgctatgtg gcagaagggg tgtaatcatg gctaaaacgt
     3181 tatacgaaaa attattcgac gctcacgtag tgtacgaagc cgaaaacgaa accccgctgt
     3241 tatatatcga ccgccacctg gtgcatgaag tgacctcacc gcaggcgttc gatggtctgc
     3301 gcgcccacgg tcgcccggta cgtcagccgg gcaaaacctt cgccactatg gatcacaacg
     3361 tctctaccca gaccaaagac attaatgcct gtggtgaaat ggcgcgcatc cagatgcagg
     3421 agctgattaa aaactgcaaa gaatttggcg tcgagctgta tgacctgaat cacccgtatc
     3481 aggggatcgt ccacgtaatg gggccggaac agggcgtcac cttgccgggg atgaccattg
     3541 tctgcggcga ctcgcatacc gccacccacg gcgcgtttgg cgcactggcc tttggtatcg
     3601 gcacttccga agttgaacac gtactggcaa cacaaaccct gaaacagggc cgtgcgaaga
     3661 ccatgaaaat tgaagtccag ggcaaagccg cgccgggcat taccgcaaaa gatatcgtgc
     3721 tggcaattat cggtaaaact ggcagcgcag gcggcaccgg gcatgtggtg gagttttgcg
     3781 gcgaagcaat tcgtgattta agcatggaag gtcgtatgac cctgtgcaat atggcgatcg
     3841 aaatgggcgc aaaagccgct ctggttgctc ctgacgaaac cacctttaac tatgtcaaag
     3901 gccgtctgca tgcgccgaaa ggcaaagatt tcgacgacgc cgttgcctac tggaaaaccc
     3961 tgcaaaccga cgaaggcgca actttcgata ccgttgtcac tctgcaagca gaagaaattt
     4021 caccgcaggt cacctggggc accaatcccg gccaggtgat ttctgtgaac gacaatattc
     4081 ccgatccggc ttcgtttgcc gatccggttg aacgtgcgtc ggcagaaaaa gcgctggcct
     4141 atatggggct gaaaccgggt attccgctga ccgaagtggc tatcgacaaa gtgtttatcg
     4201 gttcctgtac caactctcgc attgaagatt tacgcgcggc agcggagatc gccaaagggc
     4261 gaaaagtcgc gccaggcgtg caggcactgg tggttcccgg ctctggcccg gtaaaagccc
     4321 aggcggaagc ggaaggtctg gataaaatat ttattgaagc cggttttgaa tggcgcttgc
     4381 ctggctgctc aatgtgtctg gcgatgaaca acgaccgtct gaatccgggc gaacgttgtg
     4441 catccaccag caaccgtaac tttgaaggcc gccaggggcg cggcgggcgc acgcatctgg
     4501 tcagcccggc aatggctgcc gctgctgctg tgaccggaca tttcgccgac attcgcaaca
     4561 ttaaataagg agcacaccat ggcagagaaa tttatcaaac acacaggcct ggtggttccg
     4621 ctggatgccg ccaatgtcga taccgatgca atcatcccga aacagttttt gcagaaagtg
     4681 acccgtacgg gttttggcgc gcatctgttt aacgactggc gttttctcga tgaaaaaggc
     4741 caacagccaa acccggactt cgtgctgaac ttcccgcagt atcagggcgc ttccattttg
     4801 ctggcacgag aaaacttcgg ctgtggctcc tcgcgtgagc acgcgccctg ggcattgacc
     4861 gactacggtt ttaaagtggt gattgcgccg agttttgctg acatcttcta cggcaatagc
     4921 tttaacaacc agctgctgcc ggtgaaatta agcgatgcag aagtggacga actgtttgcg
     4981 ctggtgaaag ccaatccggg gatccatttc gacgtggatc tggaagcgca agaggtgaaa
     5041 gcgggagaga aaacctatcg ctttaccatc gatgccttcc gccgccactg catgatgaat
     5101 ggtctggaca gcatcggcct gaccctgcag cacgacgacg ctattgcgtc ttatgaagca
     5161 aaacaacctg cgtttatgaa ttagacccct tgcccggtca aatgaccggg ctttccgcta
     5221 tcgtccacgt catcaaacgt ctttaacctt tgcggttaaa aataatgcga caacagaaat
     5281 aatcgcaatt acccagtaga cagcaaagtg cccccaactt tgtgcaattg ctccctgaat
     5341 aacaccagcc agaattaccc cggtagagat agtgttggta aataaggtgg tagctgcccc
     5401 cgctcttcca ggcattaaat cctgaaacca tagcatccca atacccgcaa caatgccgat
     5461 aaatacagcg ttaaaaagtt gcagcgtcat caacgccata cggctatgaa agaaaatcaa
     5521 tccagtgtaa aacagcactc ctgccaccac tgcaatgacc atcattcgcc gcttaccata
     5581 acgtttgaca tagtagccag ccagaatcat tgctggtatt tccagtccag ctgccgtccc
     5641 catcaggaaa cccgccagtt tgtctggcaa tcctaaatcg ctactgatcc acaatggcat
     5701 atcaataatg tacatagtgt tgcaggtcca cattaacgtc gaggcgacaa ataacatccg
     5761 tacgttacta tcctgccagt caccttgcat tgataaagca ttttccgacg gcagttctac
     5821 ccgcgccaca gacagaagca taaatgcaat caataccaga ctgagtgtga atatcccggc
     5881 ggcaatcgaa aacatcactg taaagccgta attcaacgcc agcataaagg ccagcggtgg
     5941 accgattacc catgccagag aaagctgcgc acgcatcacc gagctaaaca tcaccacttc
     6001 tcgcgccgag ttatccgcgt attcccgcgc cagagcaaat aactgtggca ttgccgtatt
     6061 ggccagagat gccagaagca caccacaggt gataagcgtc agataatgac gattaaatgc
     6121 aaacagtagc gcattgccaa tagccatcaa acagcaaaat ataatcagtt ttcgccgatc
     6181 gccctgactg tcagaacgtt ttgccaacca gaggcttacg ccgatcccag caatagcatt
     6241 caccgtataa aagagtccga tccagaaagg ttgcgcgcca acctcacgac tcagaaataa
     6301 gctcaatgta ggagcctgta gcgccccggc cacccccatc ataaaagcga ccagcataaa
     6361 tgccgcgtaa acaccgttca tacggcgagc catcgtcatt atccagatca tacgttccct
     6421 ttttagcgcg gcgagaataa aaaaaaccag caggtataat ctgctggcgg gtgattttac
     6481 accaatactc agtcacacat gatgcaggca agtcaacttt cagaattgcg gtcatcccac
     6541 tgcatcagtc cttcaagcat ggttaatcgt tgtgggacgc ttaaccaacg caaccagcac
     6601 aacttcgctg tcgcggtaaa atagtgctga taaaactgac gcatggggca cctccttgct
     6661 tcatcgttag gaattattga cttaatatag ggaaaataaa attgctgtct tttgcgcagg
     6721 agttcccctt ttatgccatc tgctcgtctg caacaacagt tcatccgcct gtggcaatgc
     6781 tgcgagggta aatcgcagga cacaacgctc aacgaactgg cagcgttatt gagctgctcg
     6841 cgtcgtcata tgcgtaccct gctcaacacc atgcaggatc gcggctggct gacgtgggaa
     6901 gcggaagtcg ggcgcggtaa acgctcgcgt ctgacattcc tctataccgg gctggcgctt
     6961 cagcaacagc gggcggaaga cctgctggag caggatcgta tcgaccaact agtgcagttg
     7021 gttggcgaca aagcgactgt gcggcaaatg ctggtttctc atctgggccg cagcttccgc
     7081 caggggcggc acatcctgcg cgtgctctac tatcgtccgt tgcgtaatct gctacctggc
     7141 agcgcattgc gccgttccga aacccatatt gcccggcaaa tcttcagttc gctaacgcgc
     7201 ataaatgagg aaaatgggga actggaagca gacatcgccc accactggca gcaaatatca
     7261 ccgcttcact ggcgtttctt tttgcgtcca ggagtccatt ttcatcatgg tcgtgaactg
     7321 gaaatggacg acgtgatcgc ctctttaaaa cgaatcaata cgctgccgct ctattcgcat
     7381 attacggaca ttgtgtcgcc gacgccctgg acgctggata tccatctcac gcagccggac
     7441 cgctggttgc cgttactgct ggggcaagtt ccggcgatga tcctgccgtg cgaatgggaa
     7501 accctcagta actttgccag ccatcctatt ggcaccggtc cgtatgcggt gattcgcaac
     7561 agcaccaatc aactgaaaat tcaggcattc gatgacttct tcggttaccg ggcattaatc
     7621 gacgaagtta acgtctgggt tctgccggaa attgccgacg agccagccgg tggtctgatg
     7681 ctaaaaggtc cacagggcga gaaaaaagag attgaaagcc gcctggagga aggttgctac
     7741 tatttactgt tcgacagccg cacccatcgc ggggcgaatc agcaagtcag ggactgggta
     7801 agctatgtgc tttctccaac taatctggtc tatttcgctg aggaacagta ccagcaactg
     7861 tggttcccgg cttatggact gctcccccgt tggcaccatg cccgcaccat aaagagcgaa
     7921 aaaccggctg gcctggaaag cctcaccctg accttttatc aggatcacag tgagcatcgg
     7981 gtgattgccg ggatcatgca gcagattctg gcaagtcacc aggtcacgct ggaaatcaaa
     8041 gagatcagct acgatcagtg gcatgaagga gagatcgaga gcgatatctg gcttaatagc
     8101 gccaacttta cgctgccgct ggatttttcg ctgttcgcgc acttgtgcga ggtgccgctg
     8161 ctccaacact gtattccgat cgactggcaa gccgacgccg cccgctggcg caatggcgaa
     8221 atgaacctgg cgaactggtg ccagcaactg gtcgccagta aagcaatggt gccgcttatc
     8281 caccactggc tgatcattca gggacaacgc agtatgcgcg gcctgcgtat gaataccctc
     8341 ggctggttcg attttaaatc agcgtggttt gcgccaccgg atccatgatt gctggtagat
     8401 aacaaaatca ctacactaac gccgttctca acggggtgcc acgcaaacgc gtgcgctgag
     8461 aaaatacccg tcgaacctga tccggataac gccggcgaag ggatttgagg ctccttctca
     8521 agtcctttgc cactcttttt tgaggtgcaa agtgttaaaa aaatgtcttc ccctgctgtt
     8581 gctgtgcaca gcgcccgttt tcgctaaacc cgttctgact gtttatacct acgattcctt
     8641 cgccgccgac tgggggcctg gtccaaagat caaaaaagct tttgaagcca actgtaactg
     8701 cgaactgaaa ctggtggcgc tggaagatgg cgtttcgctt ctcaaccgtc tacggatgga
     8761 aggcaaaaat agtaaagccg atgtggtgct ggggctggat aacaatctgt tagacgccgc
     8821 cagtaaaacc ggactatttg ccaaaagcgg tgtggcagcg gatgccgtta acgttcccgg
     8881 cggctggaat aatgacactt tcgtcccgtt tgattacggc tatttcgcct tcgtctatga
     8941 caagaacaaa ctgaaaaacc cgccgcaaag cctgaaagaa ttggttgaga gcgatcagaa
     9001 ctggcgggtg atttatcagg atccgcgtac cagcacgccg gggctgggtc tgctgttgtg
     9061 gatgcaaaaa gtctatggcg atgacgcccc acaagcctgg caaaaactgg cgaagaaaac
     9121 cgtcacggtc accaaaggct ggagcgaagc ctacggcctg tttttaaaag gtgaaagcga
     9181 tctggtactg agttacacca cctctccggc ttatcacatt ctcgaagaga aaaaagataa
     9241 ctacgccgcc gcgaacttta gcgaaggtca ctatctgcag gtggaagtcg ccgcccgcac
     9301 cgctgccagc aagcagccgg agctggcgca aaaattcctc cagtttatgg tttctccggc
     9361 ttttcagaat gcgatcccaa ccggtaactg gatgtatccg gtggcaaatg tcacgctgcc
     9421 tgccgggttt gaacaattga ccaaaccggc caccacgctg gagttcacac cagctgaagt
     9481 ggcggcacaa cgtcaggcat ggattagcga atggcaacgc gccgtcagcc gttaattccc
     9541 ggctggttaa ttccaggtgt aagcgccgcc acgctggtgg tagcggttgc gctcgcggcg
     9601 tttctcgccc tgtggtggaa cgcgccgcag ggtaactggg tggcagtctg gcaggacagc
     9661 tacctgtggc atgtggtgcg cttctccttc tggcaggcgt ttctctcggc gctactctct
     9721 gtcgtacccg cgatattcct cgcccgcgcc ctctatcgca ggcgctttcc gggtcggctg
     9781 gcgctgttgc gtctgtgcgc aatgaccttg atcctcccgg tgctagttgc tgttttcggc
     9841 attcttagcg tctatggtcg tcagggctgg ctggcatcgc tctgccaatc gctcggtctg
     9901 gagtggacct tttcgcccta cggcctgcaa ggcattttgc tggcgcacgt attttttaat
     9961 ctgccgatgg cgagccgctt attactccag gcactggaaa acattcccgg cgaacaacgt
    10021 cagcttgccg cccagcttgg gatgcgcggc tggcattttt tccgcttcgt cgaatggccg
    10081 tggctacggc gacaaatccc gccggttgcc gcgcttatct ttatgctctg tttcgccagc
    10141 ttcgccaccg tgctatcgct gggcggcggt ccgcaggcaa ccactatcga gctggctatt
    10201 tatcaggcgc tgagttacga ctacgatcct gcccgcgcgg cgatgctggc gctgctccag
    10261 atggtgtgtt gccttgggct ggtgctgttg agtcagcgac taagtaaggc cattgcgccc
    10321 ggcaccacgc tgctgcaagg ctggcgcgat ccggacgatc gtctgcatag ccgcatttgc
    10381 gacacggtgt taatagtgct ggcgctgctg ctgttgctgc caccgttgct ggcggtgatc
    10441 gtcgatgggg taaatcgcca gttgccggaa gtgctggcac aaccggtgct gtggcaggcg
    10501 ctgtggacct cgttgcgtat tgcgctggcg gcaggtgtat tgtgcgtagt gctgaccatg
    10561 atgctgctat ggagcagtcg cgaactgcgg gcgcggcaga aaatgctggc gggtcaggcg
    10621 ctggagatga gcggcatgtt gatcctcgcc atgccgggga ttgtgctggc taccggcttc
    10681 tttttactgc tcaacaacac tatcggcctg ccacaatctg ctgacggcat tgtgattttc
    10741 accaatgcgt taatggcgat cccttatgcg ctgaaagtac tggaaaaccc gatgcgcgat
    10801 atcaccgccc gctacagtat gttgtgtcag tcgctgggca ttgaaggctg gtcgcgctta
    10861 aaagtggtcg agctgcgcgc cctgaaacgt ccactggcgc aggcgctggc ttttgcctgc
    10921 gtgctgtcga ttggtgattt tggcgtagtg gcgttgttcg gtaacgatga tttccgtacc
    10981 ctgccgtttt atctctacca gcaaattggc tcctatcgca gccaggacgg cgcggtcacc
    11041 gcgttaattc tgctactact ctgttttctg ctgtttaccg tgattgaaaa actaccgggg
    11101 cgaaatgtta aaactgactg atatcacctg gctttatcac catttgccga tgcgttttag
    11161 cttaacggta gaacgcggcg agcaggtggc aatcctcggg ccaagcggcg cgggtaaaag
    11221 taccctgctg aatttgattg ccggttttct gacgccagcc agcggttcgc tgactatcga
    11281 tggtgttgat cacaccacga cgccgccgtc acgccgtccg gtgtcgatgc tgtttcagga
    11341 gaacaacctg ttcagccatc tgacggtcgc gcagaacatc gggctggggc taaatccggg
    11401 gttgaaactg aacgcggcac agcaggagaa aatgcacgcg attgcccgtc agatggggat
    11461 tgataattta atggcgcggt taccgggcga gctttccggc ggtcagcgac agcgcgtggc
    11521 gttagcgcgt tgtctggtgc gcgaacagcc gattttattg ctcgatgaac cgttctctgc
    11581 gctcgatccg gcgttacgtc aggagatgct gacgctggtg agcacgagct gccagcagca
    11641 aaaaatgacg ctattgatgg tgtcgcacag tgtggaagat gcggcgcgga tcgccacacg
    11701 ctcggtggtg gtcgccgacg ggcgcatcgc ctggcagggt aagactgaag aattgttgag
    11761 cggtaaggcg agtgcttcgg caatattggg gattacgggt tagtatgcac tgtcagatgc
    11821 ggcgtgaaca ccttatccga cctacggaca gtgcgttttg taggcctgat aagacgcggt
    11881 cagcgtcgca tgaggcaacc cgcacaagac aatcccctaa accccaacca ctttacgcaa
    11941 aatatcgata tacaccggca tcaacgggtg gcgaattaac accaccagcg ccaccacgcc
    12001 gatggcagaa atcaacggcg tcagccacaa caaacggccg cgcgacaaat aatgacttaa
    12061 acgatcggtt gctttaccgc tgcgccataa ccgccagcac agccagccac caacccacaa
    12121 aaataccgcc gtcgccagca gcaaccattt aaactcgccg ctctgcattc cggctggaat
    12181 atcgatcgcc gcgcccgcca gaatcccagg caggaagtaa aacggtggcc acagcaggca
    12241 gccgataata ttcggcgtaa tgaattttgc caccggcaga tccagcattc ccgccaccat
    12301 tggcaccagc ggacgcgtcg ggccaacaaa gcgaccgacc agaatggtga acatgctgtg
    12361 ttgatgcaac gcatgttcag ttttatcgag taatgctttg tttttcttca gaaatgacca
    12421 gcgatgcaac ggctttttaa aacgccagcc cagccagaag gagatccagt cgcccagcag
    12481 acaaccgaca atccctgcca gccaagcgtg ccagaaactt aactcgccgc tgccaatcag
    12541 cgctcccagc cccgccatca gcaccgtacc gggtagaatc aaaccgacca gcgccagcga
    12601 ctccagaaag gccaccaaca ccaccgccat caatgaatac acggtagatt gggtaataaa
    12661 gtgttccagc aatgcttgca taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa
    12721 ccctatgcta ctccgtcaag ccgtcaattg tctgattcgt taccaattat gacaacttga
    12781 cggctacatc attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg
    12841 tgcatttttt aaatacccgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga
    12901 cggtggcgat aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt
    12961 cctcgcgcca gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg
    13021 acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt
    13081 gatcgctgat gtactgacaa gcctcgcgta cacgattatc catcggtgga tggagcgact
    13141 cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag cagattcatc gccagcagct
    13201 ccgaatagcg cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat
    13261 acggctggtg cgcttcatcc gggcgaaaga aacccgtatt ggcaaatatt gacggccagt
    13321 taagccattc atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc
    13381 gagcctccgg atgacaaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac
    13441 ccggtcggca gacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga
    13501 gattgagaat ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt
    13561 tggcctcaat cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca
    13621 ggggatcatt ttgcgcttca gccatacttt tcatactccc gccattcaga gaagaaacca
    13681 attgtccata ttgcatcaga cattgccgtc actgcgtctt ttactggctc ttctcgctaa
    13741 cccaaccggt aaccccgctt attaaaagca ttctgtaaca aagcaggacc aaagccatga
    13801 caaaaacgcg taacaaaagt gtctataatc acggcagaaa agtccacatt gattatttgc
    13861 acggcgtcac actttgctat gccatagcat ttttatccat aagattagcg gatcctacct
    13921 gacgcttttt atcgcaactc tctactgttt ctccataccc gtttttttgg atggagtgaa
    13981 acgatggcga ttgcaattgg cctcgatttt ggcagtgatt ctgtgcgagc tttggcggtg
    14041 gactgcgcca gcggtgaaga gatcgccacc agcgtagagt ggtatccccg ttggcaaaaa
    14101 gggcaatttt gtgatgcccc gaataaccag ttccgtcatc atccgcgtga ctacattgag
    14161 tcaatggaag cggcactgaa aaccgtgctt gcagagctta gcgtcgaaca gcgcgcagct
    14221 gtggtcggga ttggcgttga cagtaccggc tcgacgcccg caccgattga tgccgacggt
    14281 aacgtgctgg cgctgcgccc ggagtttgcc gaaaacccga acgcgatgtt cgtattgtgg
    14341 aaagaccaca ctgcggttga agaagcggaa gagattaccc gtttgtgcca cgcgccgggc
    14401 aacgttgact actcccgcta tattggcggt atttattcca gcgaatggtt ctgggcaaaa
    14461 atcctgcatg tgactcgcca ggacagcgcc gtggcgcaat ctgccgcatc gtggattgag
    14521 ctgtgcgact gggtgccagc tctgctttcc ggtaccaccc gcccgcagga tattcgtcgc
    14581 ggacgttgca gcgccgggca taaatctctg tggcacgaaa gctggggcgg cttgccgcca
    14641 gccagtttct ttgatgagct ggacccgatc ctcaatcgcc atttgccttc cccgctgttc
    14701 actgacacct ggactgccga tattccggtg ggcaccttat gcccggaatg ggcgcagcgt
    14761 ctcggcctgc ctgaaagcgt ggtgatttcc ggcggcgcgt ttgactgcca tatgggcgca
    14821 gttggcgcag gcgcacagcc taacgcactg gtaaaagtta tcggtacttc cacctgcgac
    14881 attctgattg ccgacaaaca gagcgttggc gagcgggcag ttaaaggtat ttgcggtcag
    14941 gttgatggca gcgtggtgcc tggatttatc ggtctggaag caggccaatc ggcgtttggt
    15001 gatatctacg cctggtttgg tcgcgtactc ggctggccgc tggaacagct tgccgcccag
    15061 catccggaac tgaaagcgca aatcaacgcc agccagaaac aactgcttcc ggcgctgacc
    15121 gaagcatggg ccaaaaatcc gtctctggat cacctgccgg tggtgctcga ctggtttaac
    15181 ggtcgtcgca cgccaaacgc taaccaacgc ctgaaagggg tgattaccga tcttaacctc
    15241 gctaccgaca ctccgctgct gttcggcggt ttgattgctg ccaccgcctt tggcgcacgc
    15301 gcaatcatgg agtgctttac cgatcagggg atcgccgtca ataacgtgat ggcgctgggc
    15361 ggcatcgcgc ggaaaaacca ggtcattatg caggcctgct gcgacgtgct gaatcgcccg
    15421 ctgcaaattg ttgcctctga ccagtgctgt gcgctcggtg cggcgatttt tgctgccgtc
    15481 gccgcgaaag tgcacgcaga catcccatca gcccagcaaa aaatggccag tgcggtagag
    15541 aaaaccctgc aaccgcgcag cgaacaggca caacgctttg aacagcttta tcgccgctat
    15601 cagcaatggg cgatgagcgc cgaacaacac tatcttccaa cttccgcccc ggcacaggct
    15661 gcccaggccg ttgcgactct ataaggacac gataatggcg atttttgata attatgaagt
    15721 gtggtttgtc attggcagcc agcatctgta tggcccggaa accctgcgtc aggtcaccca
    15781 acatgccgag cacgttgtta atgcgctgaa tacggaagcg aaactgccct acaaactggt
    15841 gttgaaaccg ctgggcacca cgccggatga aatcaccgct atttgccgcg acgcgaatta
    15901 cgacgatcgt tgcgctggtc tggtggtgtg gctgcacacc ttctccccgg ccaaaatgtg
    15961 gatcaacggc ctgaccatgc tcaacaaacc gttgctgcaa ttccacaccc agttcaacgc
    16021 ggcgctgccg tgggacagta tcgatatgga ctttatgaac ctgaaccaga ctgcacatgg
    16081 cggtcgcgag ttcggcttca ttggcgcgcg tatgcgtcag caacatgccg tcgttaccgg
    16141 tcactggcag gataaacaag cccatgagcg tatcggctcc tggatgcgtc aggcggtctc
    16201 taaacaggat acccgtcatc tgaaagtctg ccgatttggc gataacatgc gtgaagtggc
    16261 ggtcaccgat ggcgataaag ttgccgcaca gatcaagttc ggtttctccg tcaatacctg
    16321 ggcggttggc tatctggtgc aggtggtgaa ctccatcagc gacggcgatg ttaacgcgct
    16381 ggtcgatgag tacgaaagct gctacaccat gacgcctgcc acacaaatcc acggcgaaaa
    16441 acgacagaac gtgctggaag cggcgcgtat tgagctgggg atgaagcgtt tcctggaaca
    16501 aggtggcttc cacgcgttca ccaccacctt tgaagatttg cacggtctga aacagcttcc
    16561 aggtctgccc gtacagcgtc tgatgcagca gggttacggc tttgcgggcg aaggcgactg
    16621 gaaaaccgcc gccctgcttc gcatcatgaa ggtgatgtca accggtctgc agggcggcac
    16681 ctcctttatg gaggactaca cctatcactt cgagaaaggt aatgacctgg tgctcggctc
    16741 ccatatgctg gaagtctgcc cgtcgattgc cgtagaagag aaaccgatcc tcgacgttca
    16801 gcatctcggt attggtggta aggacgatcc tgcccgactg atcttcaata cccaaaccgg
    16861 tccagcgatt gtcgccagcc tgattgatct cggcgatcgt taccgtctgc tggttaactg
    16921 tatcgacacg gtgaaaacac cgcactccct gccgaaactg ccggtggcga atgcactgtg
    16981 gaaagcgcaa ccggatctgc caactgcttc cgaagcgtgg atcctcgctg gtggcgcgca
    17041 ccataccgtc ttcagccatg cgctgaacct caacgatatg cgccagttcg ccgagatgca
    17101 cgacattgaa atcacggtga ttgataacga cacccgcctg ccagcgttta aagacgcact
    17161 gcgctggaac gaagtgtatt acggatttcg tcgctaagta gccacatcag gtatgtaacg
    17221 cctgatgcga cgctgacgcg tcttatctgg cctacacgct gcgattttgt aggccggata
    17281 agcaaagcgc atccggcatt caacgcctga tgcgacgctg gcgcgtctta tcaggcctac
    17341 gcgctgcgat tttgtagacc ggataagcga agcgcatccg gcacgaagga gtcaacatgt
    17401 taaaagatct caaacgccag gtattagaag ccaacctggc gctgccaaaa cacaacctgg
    17461 tcacgctcac atggggcaac gtcagcgccg ttgatcgcga gcgcggcgtc tttgtgatca
    17521 aaccttccgg cgtcgattac agcgtcatga ccgctgacga tatggtcgtg gttagcatcg
    17581 aaaccggtga agtggttgaa ggtacgaaaa agccctcctc cgacacgcca actcaccggc
    17641 tgctctatca ggcattcccc tccattggcg gcattgtgca tacgcactcg cgccacgcca
    17701 ccatctgggc gcaggcgggt cagtcgattc cagcaaccgg caccacccac gccgactatt
    17761 tctacggcac cattccctgc acccgcaaaa tgaccgacgc agaaatcaac ggcgaatatg
    17821 agtgggaaac cggtaacgtc atcgtagaaa cctttgaaaa acagggtatc gatgcagcgc
    17881 aaatgcccgg cgttctggtc cattcccacg gcccgtttgc atggggcaaa aatgccgaag
    17941 atgcggtgca taacgccatc gtgctggaag aggtcgctta tatggggata ttctgccgtc
    18001 agttagcgcc gcagttaccg gatatgcagc aaacgctgct gcataaacac tatctgcgta
    18061 agcatggcgc gaaggcatat tacgggcagt aatgactgta taaaaccaca gccaatcaaa
    18121 cgaaaccagg ctatactcaa gcctggtttt ttgatggatt ttcagcgtgg cgcaggcagg
    18181 ttttatctta acccgacact ggcgggacac cccgcaaggg acagaagtct ccttctggct
    18241 ggcgacggac aacgggccgt tgcaggttac gcttgcaccg caagagtccg tggcgtttat
    18301 tcacgccgat caggttcccc gcgctcagca tattttgcag ggtgaacaag gctttcgtct
    18361 gacaccgctg gcgttaaagg attttcaccg ccagccggtg tatggccttt actgtcgcgc
    18421 ccatcgccaa ttgatgaatt acgaaaagcg tctgcgtgaa ggtggcgtta ccgtctacga
    18481 ggccgatgtg cgcccgccag aacgctatct gatggagcgg tttatcacct caccggtgtg
    18541 ggtcgagggt gatatgcaca atggcactat cgttaatgcc cgtctgaaac cgcatcccga
    18601 ctatcgtccg ccgctcaagt gggtttctat agatattgaa accacccgcc acggtgagct
    18661 gtactgcatc ggcctggaag gctgcgggca gcgcatcgtt tatatgctgg ggccggagaa
    18721 tggcgacgcc tcctcgcttg atttcgaact ggaatacgtc gccagccgcc cgctgctgct
    18781 ggaaaaactc aacgcctggt ttgcaaacca cgatcccgat gtgatcatcg gctggaacgt
    18841 ggtgcagttc gatctgcgaa tgctgcaaaa acatgccgag cgttaccgtc ttccgctgcg
    18901 tcttgggcgc gataatagcg agctggagtg gcgcgagcac ggctttaaaa acggcgtctt
    18961 ttttgcccag gctaaaggtc ggctaattat cgacggtatc gaggcgctga aatccgcgtt
    19021 ctggaatttc tcttcattct cgctggaaac tgtcgctcag gagctattag gcgaaggaaa
    19081 atctatcgat aacccgtggg atcgaatgga cgaaattgac cgccgtttcg ccgaagataa
    19141 acctgcgctg gcaacttata acctgaaaga ttgcgagctg gtgacgcaga tcttccacaa
    19201 aactgaaatc atgccatttt tactcgaacg agcaacggtg aacggcctgc cggtggatcg
    19261 gcacggcggt tcggtggcag cgtttggtca tctctatttt ccgcgaatgc atcgcgctgg
    19321 ttatgtcgcg cctaatctcg gcgaagtgcc gccgcacgcc agccctggcg gctacgtgat
    19381 ggattcacgg ccagggcttt atgattcagt gctggtgctg gactataaaa gcctgtaccc
    19441 gtcgatcatc cgcacctttc tgattgatcc cgtcgggctg gttgaaggca tggcgcaacc
    19501 tgatccagag cacagtaccg aaggttttct cgatgcctgg ttctcacggg agaaacattg
    19561 cctgccggag attgtgacta acatctggca cgggcgcgat aaagccaaac gtcagggtaa
    19621 taagccgctt tcgcaggcgc tgaagatcat tatgaatgcc ttttatggcg tgctcggcac
    19681 caccgcctgc cgcttcttcg atccgcgcct ggcatcgtcg atcaccatgc gtggtcatca
    19741 gatcatgcgg cagaccaaag cattgattga agcgcagggc tacgacatta tctacggaga
    19801 taccgactcg acttttgtct ggctaaaagg tgcacattcg gaagaagagg cggcgaaaat
    19861 cggtcgtgca ctggtgcagc acgttaacgc ctggtgggcg gaaacgctgc aaaaacaacg
    19921 gctgaccagc gcattagaac tggagtatga aactcatttc tgccgttttc ttatgccaac
    19981 cattcgcgga gctgataccg gcagtaaaaa gcgctacgcc gggctgattc aggagggcga
    20041 caagcagcgg atggtgttta aagggctgga aaccgtgcgc accgactgga cgccgctggc
    20101 ccagcagttt cagcaggagc tgtacctgcg tatcttccgc aacgagccat atcaggagta
    20161 cgtccgccaa accatcgaca aactgatggc gggtgaactg gatgcgcggc tggtttaccg
    20221 taaacgcctt cgccgtccgc tgagcgaata tcagcgtaat gttccgcctc atgtacgcgc
    20281 cgctcgcctt gccgatgaag aaaatcaaaa gcgcggtcgc cccttgcaat atcagaatcg
    20341 cggcaccatt aagtacgtat ggaccaccaa cggcccggag ccgctggact accaacgttc
    20401 accactggat tacgaacact atctgacccg ccagctacaa cccgtggcgg agggaatact
    20461 cccttttatt gaggataatt ttgctacact tatgaccggg caacttgggc tattttgagc
    20521 aaaaaaaaga gttcgccaga taccattttg atgcgtgacg aatgctttgc catccagtac
    20581 catagcgccc tttccattcc tggacctgaa taacaccact acctcataag cacggtagcg
    20641 ggtggttatt gcctgcaatt aaagatatag agccgaacac atatgccttt tacacttggt
    20701 caacgctgga tcagcgatac agaaagcgaa ttgggacttg gaaccgttgt cgcggtggat
    20761 gcgcgaactg tcactttact tttcccatct actggtgaaa accgtctgta cgcacgcagt
    20821 gattcccccg tgacccgcgt gatgttcaac cctggtgata ccattaccag ccatgacggc
    20881 tggcagatgc aagtcgaaga agtaaaagaa gaaaatggct tgctgaccta tatcggtact
    20941 cgcctggata ctgaagagtc cggcgtagcc ctgcgtgaag ttttccttga tagcaaactg
    21001 gtgttcagca aaccgcagga ccgtctgttt gccgggcaga ttgaccgtat ggaccgcttt
    21061 gcgctgcgtt atcgcgcgcg taagtattcc agcgaacagt tccgtatgcc gtacagcggc
    21121 ctgcgcggtc agcgtaccag cctgatcccg catcagctca acatcgctca tgatgttggt
    21181 cgccgccacg cgccgcgcgt cctgctggct gacgaagtgg gtttagggaa aaccattgaa
    21241 gccgggatga tcctgcatca gcaactgctc tctggcgctg ctgaacgtgt gctgattatc
    21301 gtcccggaaa ccttacagca tcagtggctg gtagaaatgc tgcgccgttt caacctgcgc
    21361 tttgctctgt ttgatgatga gcgttatgcc gaagctcagc acgatgctta caacccgttt
    21421 gacaccgaac agctggtgat ttgctcgctg gattttgccc gtcgtagcaa acagcgcttg
    21481 gaacatctct gtgaagccga atgggacctg ctggtggtcg atgaagcgca tcacctggtg
    21541 tggagcgaag atgcgccaag ccgtgaatat caggccattg aacaactggc agagcacgtg
    21601 ccgggcgttc tgctgctgac cgcgaccccg gaacagctgg ggatggaaag ccacttcgcc
    21661 cgtctgcgtc tgctggaccc gaaccgtttc cacgattttg cgcagttcgt tgaagagcag
    21721 aaaaattatc gtccggttgc ggacgccgtt gccatgctgc tggcaggtaa caaactgagc
    21781 aatgacgaac tgaacatgct cggcgagatg atcggcgagc aggatatcga gccgctgttg
    21841 caggcagcaa acagcgacag cgaagatgcc cagagcgccc gtcaggagct ggtttcgatg
    21901 ctgatggatc gccacggcac cagccgcgtg ctgttccgta acacgcgtaa cggtgtgaaa
    21961 ggattcccga aacgcgagct gcacaccatt aagctgccgc taccgacgca gtatcagacg
    22021 gctattaaag tctccggcat tatgggcgca cgtaaaagtg cggaagatcg tgctcgcgat
    22081 atgctctacc cggagcgtat ttatcaggaa tttgaaggtg ataacgccac ctggtggaac
    22141 ttcgatccgc gcgttgagtg gctgatgggc tacctgacca gccatcgctc tcagaaagtg
    22201 ctggtgatct gcgccaaagc tgccactgcg ctgcaactgg agcaggtact gcgcgaacgt
    22261 gaaggtattc gcgctgcggt gttccacgaa ggtatgtcga ttatcgaacg tgaccgcgct
    22321 gccgcctggt ttgccgaaga agacaccggc gcacaggtac tgctgtgctc agaaatcggt
    22381 tctgaaggac gtaactttca gttcgccagt cacatggtga tgtttgacct gccattcaac
    22441 ccggatctac tggagcagcg tattggtcgt ctggatcgta tcggccaggc gcacgatatt
    22501 cagatccatg tgccttatct ggagaaaacc gctcagtcgg tgctggtgcg ctggtatcac
    22561 gaaggtctgg atgcatttga gcacacctgc ccgaccggac gcactattta cgatagcgta
    22621 tacaacgatc tgattaacta tctggcttca ccggatcaaa ccgaaggctt tgacgatctg
    22681 atcaaaagct gccgcgagca acatgaagcg ctgaaagcac agctggaaca gggtcgtgac
    22741 cgcctgctgg aaatccactc caacggtggc gaaaaagccc aggcactggc agaaagcatt
    22801 gaagagcagg atgacgatac caacctgatc gccttcgcca tgaacctgtt cgatattatc
    22861 ggtatcaatc aggacgatcg cggcgacaac atgatcgtgc tgacgccgtc cgatcatatg
    22921 ctggtgccgg acttccctgg cctgtcggaa gatggcatca ccatcacctt tgatcgtgaa
    22981 gtggcgctgg cgcgtgaaga tgcacagttt attacctggg agcatccgct gatccgcaac
    23041 ggtctggatc tgatcctttc tggcgatacc ggtagcagca cgatttcact gttaaaaaac
    23101 aaagcgttgc cggtaggtac gctgttggtg gaactgattt atgtggttga agcccaggct
    23161 ccgaagcagt tgcagctcaa ccgcttcctg ccaccgacgc cggtacgtat gctgctggat
    23221 aaaaacggca acaacctggc ggcgcaggta gagtttgaaa cctttaaccg ccagcttaac
    23281 gcggttaacc gtcacaccgg cagcaaactg gttaacgccg tgcagcagga tgttcacgct
    23341 atccttcaac tgggtgaagc gcagatcgag aaatctgccc gtgcattgat tgatgcagcg
    23401 cgtaacgaag ccgacgaaaa actgtctgcc gagctgtctc gtctggaagc tctgcgtgca
    23461 gtgaacccga acattcgtga cgacgaactg accgccattg agagcaaccg tcagcaggta
    23521 atggaaagcc tggatcaggc aggttggcgt ctggatgccc tgcgtttgat cgttgtaacg
    23581 catcagtaac ggagccgaaa atggggatgg aaaactacaa tccaccgcag gaaccctggt
    23641 tggttatcct gtatcaggat gaccatatta tggtggtcaa caagccgagc ggtttgttgt
    23701 cagtgccggg tcgtctggaa gagcacaaag acagcgtgat gacgcgcatt cagcgtgatt
    23761 atccgcaggc agaatcggtg catcgtctgg atatggctac cagcggcgtg attgtagtgg
    23821 cgctgaccaa agccgcggag cgggagttaa aacgccagtt ccgcgagcgc gagccgaaaa
    23881 agcagtatgt ggcccgcgtc tgggggcatc catcccccgc agaaggtctg gtggatctgc
    23941 cgctgatttg cgactggcca aaccgcccga aacagaaagt ctgttacgaa acgggtaaac
    24001 ctgcgcagac ggaatatgaa gtggtggagt atgcggcgga taacacggca agagtggtgt
    24061 taaaaccgat taccgggcgt tcgcatcaat tgcgtgtgca tatgctggcg ctggggcatc
    24121 cgattctcgg cgatcgtttt tatgcatcac cagaagcgag agcgatggca ccgcgtttgt
    24181 tgctacatgc agagatgttg acgattaccc atccggcgta tggcaatagt atgacgttta
    24241 aagcgccagc ggatttttaa tcgtcacgct ggcgcagttc ggtaggcgga taaggcgttc
    24301 acgccgcatc cggcaattgt gcgccgattg cctgatgcga cgcgggccgc gtcttatcag
    24361 gcctacagtt taaaacttat ttaaaccctt tctgctgctt tatcagctca tatgcctgct
    24421 gaatttcctg cgctttctgc ttcgccatct ccatcatctc aggcggcaaa cctttcgcca
    24481 ccagcttatc gggatggtgt tcactcatca gcttacggta ggcacgtttg atggtggtcg
    24541 catcatccgt cggcttcacg cccagcacat tacaggcatc ttccagcgtc gggccacgct
    24601 gcgcttgctg ccagttacca ccgccagatt gctgctgata accgccgcca aactgtgcac
    24661 cgccctgcat catgcgcaaa aactggtcaa actgggcgcg ggagatccct aattcttcag
    24721 caatgacata cagcaccgcc cgttcattcg ggtgcagtga accatcagca aacgccgcct
    24781 gaatctggat ctccagaaac atacgaatta agtcaaaacg accaaagcag acactgcgaa
    24841 actggcgcat cttttcgcgc agcgggtaat tatctgattt tcccacccgg aacgcgtttt
    24901 gcgccgcagt acgggaagcg ccatgaaggt tcattcggtc catcaactgg ctggcgatat
    24961 gaatatcagc ctccgtgacg cgacctttgg atttggttaa atgccccatc acttcaaaag
    25021 tggtggcaaa aaacagcgcc tgacgctcac gctggttggc gaaccacgcc attttacggc
    25081 tacgggcttt atcaaacata tggccaatta acaggcctaa cactacgccc caaaagccgc
    25141 cgcccatcag taaggccacg gccacgccaa tgatttttcc ccaatactgc atatattccc
    25201 caaatcgaca cacggatatc agggctatct cccacaatat aaaggtgctt ttaccgtttt
    25261 ccggctgcgg tcaattgtgg gacatcgcct ataatttgca ttatcatacc tgtcattcaa
    25321 tgccgtgcct aacaccactg acgctattcg gacaggatta acactagcgt agagatgacg
    25381 agtacgttag tctctgaccg tttgtcacgc gcaacgttac cgatgatgga acaataaaat
    25441 caacgtatga aaaaacgtat ccccactctc ctggccacca tgattgccac cgccctttat
    25501 agtcaacagg gactggcagc cgacctcgcc tcacagtgca tgttgggcgt gccaagctat
    25561 gaccgtcctc tggtacaggg cgataccaat gacttacccg tgactatcaa tgctgaccac
    25621 gcgaaagggg actacccgga tgacgccgtg tttactggca gcgtggatat catgcagggt
    25681 aacagccgtc tgcaggccga cgaagtgcag ctccatcaaa aagaggcacc aggacaaccg
    25741 gagccggtac gtaccgttga tgcgctcggt aatgtccatt acgacgataa ccaggtgatc
    25801 ctcaaagggc cgaaaggctg ggcgaatctg aacaccaaag ataccaacgt ctgggaaggt
    25861 gattaccaga tggtgggtcg ccagggtcgc ggtaaagcgg acctgatgaa acaacgtggc
    25921 gaaaaccgct ataccattct ggataacggt agctttacct cctgtctgcc gggttctgac
    25981 acctggagcg tggtaggtag cgaaattatt catgaccgcg aagaacaagt tgctgagatc
    26041 tggaacgccc gctttaaggt gggtccggta ccgatctttt atagccccta tttgcagttg
    26101 ccggtgggtg acaaacgtcg ctctggtttc ttgatcccga acgccaagta caccaccacc
    26161 aactactttg agttctacct gccatattac tggaacatcg cgccaaatat ggatgccacc
    26221 atcacgccgc attatatgca tcgtcgtggc aacatcatgt gggagaacga attccgctac
    26281 ctctcccagg cgggcgctgg cttgatggaa ctggactatc tgccttcaga taaagtctat
    26341 gaagatgaac acccgaacga tgacagttca cgtcgttggt tattctactg gaaccactcc
    26401 ggggtcatgg atcaggtgtg gcgtttcaac gtcgactaca ccaaggtcag cgatcctagc
    26461 tacttcaatg atttcgataa caagtacggt tccagtactg acggctacgc aacgcaaaaa
    26521 ttcagcgttg gctatgcggt gcaaaacttc aatgccaccg tttcaaccaa gcagttccag
    26581 gttttcagcg aacagaacac cagtagctac tcggcagagc cgcagttaga cgttaattac
    26641 taccagaatg atgttggtcc gtttgatacg cgtatttacg gccaggcagt gcactttgtt
    26701 aacaccagag acgacatgcc tgaagcaacc cgtgttcacc tggaaccgac catcaatttg
    26761 ccgctctcta ataactgggg cagcatcaat accgaagcga agtttctggc aacccattat
    26821 cagcaaacca atcttgactg gtataactcc agaaacacga ccaagctgga cgaatccgtt
    26881 aaccgcgtaa tgccgcaatt caaagttgac ggcaaaatgg tctttgaacg cgatatggaa
    26941 atgctggctc cgggttatac ccaaacgctg gaaccgcgcg cgcagtattt gtacgtgccg
    27001 tatcgcgatc agagcgacat ctataactac gactcgtctc tgctgcaatc tgactactct
    27061 ggcctgttcc gggaccggac ttacggcggt cttgaccgta ttgcctccgc taaccaagtg
    27121 acgaccggtg tcacatctcg catatatgat gatgctgccg ttgaacgttt taatatttcc
    27181 gttggtcaaa tctactattt cacggagtct cgcactggcg atgacaacat aacatgggag
    27241 aatgacgaca aaacgggctc actggtgtgg gcaggcgaca cctactggcg tatctccgag
    27301 cgttggggat tgcgtggcgg gattcagtac gatacacgtc tggataacgt agcgaccagt
    27361 aactccagca ttgaataccg tcgggatgaa gaccgtctgg tacagctgaa ttaccattac
    27421 gccagcccgg aatatattca ggctacgctg cctaagtact attccactgc tgagcaatat
    27481 aagaatggta tttcgcaggt aggtgctgtc gccagcaggc caattgccga tcgttggtcc
    27541 attgttgggg cctactacta cgacaccaat gcgaacaagc aagccgactc tatgttaggt
    27601 gtgcaataca gctcctgctg ctatgcaatt cgcgtcggtt acgagcggaa gctgaacggt
    27661 tgggataacg ataaacaaca tgcggtatat gacaacgcaa tcggctttaa catcgaactt
    27721 cgcggcctga gctccaacta cggtctgggt acgcaagaga tgctgcgttc gaacattctg
    27781 ccgtatcaaa acactttgtg atttgttgat ttaccacgta atccgcagtg cggttaattg
    27841 aaatggaaaa agtatgaaga actggaaaac gctgcttctc ggtatcgcca tgatcgcgaa
    27901 taccagtttc gctgcccccc aggtagtcga taaagtcgca gccgtcgtca ataacggcgt
    27961 cgtgctggaa agcgacgttg atggattaat gcagtcggta aaactgaacg ctgcgcaggc
    28021 aaggcagcag cttcctgatg acgcgacgct gcgccaccaa atcatggaac gtttgatcat
    28081 ggatcaaatc atcctgcaga tggggcagaa aatgggagtg aaaatctccg atgagcagct
    28141 ggatcaggcg attgctaaca tcgcgaaaca gaacaacatg acgctggatc agatgcgcag
    28201 ccgtctggct tacgatggtc tgaactacaa cacttatcgt aaccagatcc gcaaagagat
    28261 gattatctct gaagtgcgta acaacgaggt gcgtcgtcgc atcaccatcc tgccacagga
    28321 agtcgaatcc ctggcgcagc aggtgggtaa ccaaaacgac gccagcactg agctgaacct
    28381 gagccacatc ctgatcccgc tgccggaaaa cccgacctct gatcaggtga acgaagcgga
    28441 aagccaggcg cgcgccattg tcgatcaggc gcgtaacggc gctgatttcg gtaagctggc
    28501 gattgctcat tctgccgacc agcaggcgct gaacggcggc cagatggggt ggggccgtat
    28561 tcaggagttg ccggggatct tcgcccaggc attaagcacc gcgaagaaag gcgacattgt
    28621 tggcccgatt cgttccggcg ttggcttcca tattctgaaa gttaacgacc tgcgcggcga
    28681 aagcaaaaat atctcggtga ccgaagttca tgctcgccat attctgctga aaccgtcgcc
    28741 gatcatgact gacgaacagg cccgcgtgaa actggagcaa attgctgctg atatcaagag
    28801 tggtaaaacg acttttgctg ccgcagcgaa agagttctcg caggatccag gttctgctaa
    28861 ccagggcggt gatctcggct gggctacacc agatattttc gatccagcct tccgtgacgc
    28921 cctgacccgc ctgaacaaag gtcaaatgag tgcaccggtt cactcttcat tcggctggca
    28981 tttaatcgaa ctgctggata cccgtaatgt cgataaaacc gacgctgcgc agaaagatcg
    29041 tgcataccgc atgctgatga accgtaagtt ctcggaagaa gcagcaagct ggatgcagga
    29101 acaacgtgcc agcgcctacg ttaaaatcct gagcaactaa tggttaaaac ccaacgtgtt
    29161 gtgatcactc ccggcgagcc cgccgggatt ggcccggact tagttgtcca gcttgcacag
    29221 cgtgagtggc cggtcgaact ggttgtttgt gccgatgcca ctctccttac cgaccgggca
    29281 gcgatgctcg gtttgccgct caccctccgc ccttattccc ccaactcccc tgcacaaccg
    29341 caaactacgg gcacattaac gctacttcct gtcgcgctac gtgaatctgt cactgcgggg
    29401 cagttagcga ttgaaaatgg gcattacgtg gtggagacgc tggcgcgagc gtgtgatggc
    29461 tgtctgaacg gtgaatttgc tgcgctgatc acaggccccg tgcataaagg cgtcattaac
    29521 gacgcaagca ttccgtttac cggtcatacc gagtttttcg aagagcgttc gcaggcgaaa
    29581 aaagtggtga tgatgctggc gaccgaagaa cttcgcgtgg cgctggcaac gacgcattta
    29641 ccgctgcgcg atatcgcaga tgctatcacc cctgcacttt tgcacgaagt gattgctatt
    29701 ttgcatcacg atttgcgtac aaaatttggt attgccgaac cgcgcattct ggtctgcggg
    29761 ctgaatccgc acgcgggcga aggcggtcat atgggtacgg aagagataga caccattatt
    29821 ccggtgctcg acgagctgcg ggcgcagggg atgaaactca acgggccgct gcctgccgat
    29881 accctgtttc agccgaaata tctcgataac gccgacgccg tgctggcgat gtaccacgat
    29941 cagggtcttc ccgtgctaaa ataccagggc ttcgggcgcg gtgtgaacat tacgctgggc
    30001 ctgcccttta ttcgcacatc agtggaccac ggcaccgcgc ttgaactggc gggacgtggc
    30061 gaagccgatg tcggcagttt tattacggcg cttaatctcg ccatcaaaat gattgttaac
    30121 acccaatgaa taatcgagtc caccagggcc acttagcccg taaacgcttc gggcaaaact
    30181 ttctcaacga tcagttcgtg atcgacagca ttgtttctgc cattaacccg caaaagggcc
    30241 aggcaatggt cgaaatcggc cccggtctgg cggcattgac cgagccggtc ggcgaacgtc
    30301 tggaccagct gacggtcatc gaacttgacc gcgatctggc ggcacgtctg caaacgcatc
    30361 cattcttagg cccgaaactg acgatttatc agcaggatgc gatgaccttt aactttggcg
    30421 aactggccga gaaaatgggt cagccgctgc gtgttttcgg caacctgcct tataacatct
    30481 ccacgccgtt gatgttccat ctgtttagct atactgatgc cattgccgac atgcacttta
    30541 tgttgcaaaa agaggtggtg aatcgtctgg ttgcaggacc gaacagcaaa gcgtatggtc
    30601 gattaagcgt catggcgcaa tactattgca acgtgatccc ggtactggaa gttccgccgt
    30661 cagcctttac accaccaccc aaagtggatt ccgccgtcgt gcgcctggtc cctcatgcaa
    30721 cgatgcctca cccggttaaa gatattcgcg tgttgagccg catcaccacc gaagccttta
    30781 accagcgtcg taaaaccatt cgtaacagcc tcggcaacct gtttagcgtc gaggtgttaa
    30841 ccggaatggg gatcgacccg gcgatgcgag cggaaaatat ctctgtcgcg caatattgcc
    30901 agatggcgaa ctatctggcg gagaacgcgc ctttgcagga gagttaacga tgatcaattc
    30961 gccccgagtg tgtattcagg ttcaaagcgt ctacattgag gcacaatctt cacctgataa
    31021 tgaacgttac gtttttgctt ataccgtaac catacgcaat ctggggcgag cgccagtgca
    31081 gttgttgggg cgttactggc tgatcaccaa tggcaatggc cgtgaaaccg aagtccaggg
    31141 cgaaggagtg gttggcgtcc agccacttat cgcgcctggc gaagagtacc agtacaccag
    31201 cggtgcaatc attgaaaccc cgctgggcac catgcagggt cactacgaaa tgatcgatga
    31261 aaatggcgtc cctttcagca tcgacattcc cgtattccga ctcgccgttc ccacactcat
    31321 tcattaaaag aatatggcga cataccttat tggcgacgtt catggttgtt acgatgaact
    31381 gatcgcattg ctgcataaag tagaatttac ccccgggaaa gataccctct ggctgacggg
    31441 cgatctggtc gcgcgcggcc cgggttcgct ggatgttctg cgctatgtga aatccttagg
    31501 cgacagcgta cgtctggtgc tgggcaatca cgatctgcat ctgctggcgg tatttgccgg
    31561 gatcagccgc aataaaccga aagatcgcct gacaccgctg ctggaagcgc cggatgccga
    31621 cgagctgctt aactggctgc gtcgccagcc tctgctgcaa atcgacgaag agaaaaagct
    31681 ggtgatggcc cacgcaggga tcacgccgca gtgggatctg cagaccgcca aagagtgcgc
    31741 gcgcgatgta gaagcggtgc tgtcgagtga ctcctatccc ttctttcttg acgctatgta
    31801 cggcgatatg ccaaataact ggtcaccgga attgcggggg ctgggaagac tgcgttttat
    31861 caccaacgcc tttacccgta tgcgtttttg cttcccgaac ggtcaactgg atatgtacag
    31921 caaagaatcg ccggaagaag cccctgcccc actgaaaccg tggtttgcga ttcctggccc
    31981 tgtcgctgaa gaatacagca tcgcctttgg tcactgggca tcgctggaag gcaaaggtac
    32041 gccggaaggt atttacgcgc tggataccgg ctgctgctgg ggtgggtcat taacctgcct
    32101 gcgctgggaa gataaacagt attttgtcca gccgtcgaac cggcataagg atttgggcga
    32161 agcggcggcg tcttaaacac agcctgatac aggaaggccg gataagacgc gaccggcgtc
    32221 gcatccggca ctagccgtaa attctataca aacttaccgc cgctccagaa tctcaaagca
    32281 atagctgtga gagttctgcg catcggcgtc gtggaattcg ctgaataccg attcccagtc
    32341 atccggctcg taatccggga aatgggtgtc gccttccact tctgcgtcga tatgcgtcag
    32401 atacagtttt tgcgcttttg gcaggaactg ctcataaacg cgaccgccgc caatcaccat
    32461 gatttctggt acgtcaccac acgccgcaat ggcttcatcc accgatttca cccacgttac
    32521 gcgatcgtcc gtacccggtt gactgctgag gataatattt ttgcgtcctg gcaacggacg
    32581 accgattgat tcccaagtat ggcggcccat aatcacgggt ttatttaagg tgttgcgttt
    32641 aaaccaggcg agatcggcag gcaggttcca cggcatggcg ttttccatgc cgataacgcg
    32701 atctaccgct aacgccgcaa tcagactgat cattgagatt tcccgataaa aaaattgtcg
    32761 ccactatacg taaagcgtaa accgtcgtcg actggtgcga ggatgatgtt gaggaaaatt
    32821 ttatattctg ctggcgagtc cacgctctcc ccctggactc gccgcattac aatgaaacaa
    32881 aaacaaacag ttagctgtaa agtgtgattt acgtcactct ttattaagat gagggtttcg
    32941 tttccggttc atccgccatg ttgccggtat gtttaccttc ttctgttccc tgccagccat
    33001 gtcgttgaat taatgacaga tgttcgcggt cctcggtgat gatctcactt aacatcgcgc
    33061 tggtgcgttt atagaccgcc gcgcgggctt tggtgtcatt ctcaaccatt gccatctctt
    33121 ccaccatctg aatattaaag cggcggaaca catcggcacg ttctcgcgct tcatacggcc
    33181 ccagacctaa actttccagc gccagacgcc cggttttcag cgcaccttca aaggtttcac
    33241 gctccggctt ttcaacgcct gcctgacgca aacgaatgta gtgatcgaca tcgcgggcgc
    33301 gggcaataat ctgcaaatgc gggaaatgtt ctttcaccat ctccgtcagt tgcaggttgg
    33361 tttgcggatc gtcaatagcg ttaatcagca cttccgcttt cgccgctccg gcagattcca
    33421 gtaaatccat ccgcgtggca tcgccataaa acactttcat accaaattta cgcagcgttt
    33481 cgatatggtc cggatcgtga tcgagcacca ccattttcac cccgctggag agcagtaaac
    33541 gtccggtaat ctgtccaaaa cgaccgaatc cggcgataat cacgcgcggc tgttcttcat
    33601 cgatctcatc ggcttcacgc gcctcctccg tagaagattg ctcaaggcga ttgaggatca
    33661 ccagcagaat cggcgttgct gccatcgaca gcgccaccgc cagggtcagc gatttcaccc
    33721 actccggctc cagcacattc gccatctgcg ccgcaccaaa taccacaaag gcaaactcac
    33781 tgccctgccc taacaacacc gcaaaccagc gacgctgttt atttggcacc tgcaacggtc
    33841 gggcaatcag ccacagcatg gcgattttga tgatgaggaa accgagcagc aaaatgacaa
    33901 tgcgcaatgg gttttcaatc agcgtgccaa agtctatcga catgccaaca ccgatgaaaa
    33961 acagccccaa cagcaaacct ttaaacggtt cgatatcgct ttccaacgca tgacggtatt
    34021 ccgagcttgc cagcagtacg cccgccagaa acgcgcccat cgccatcgac aagccgacct
    34081 cttccagcag caaaccaaag ccaaacacaa ggaataacgc cacggcacta aacacttccc
    34141 gcaagccaga gcgggcaaca aaacgcagcg ccggacgcgt aacatagcgc cccagcaata
    34201 ccaccagtac cagcgcaccc gccactttta acgccgagag agcaaatgcg cccatcgtcg
    34261 tcgaggcact gctcgccgcc agtagcggga tcatcgccac cagcgggatt gccgcgatat
    34321 cctggaacag cagcactgca aaggcactgc ggcccatttg cgtcaccatc agattgcgtt
    34381 cattcatcgc ctgcatggca atcgccgttg atgagagcgc cagcgtcata ccgatcaatt
    34441 ccgcgacctg ccagcgcagc ccaagtaaca tacagaacag ccccagcagg ccaccgcaaa
    34501 tcaccatctg caatgcgcca ccgccgaaca ctgccgcacg cagcttccac agcctttgtg
    34561 gatcgagttc gaggccgata ataaacagca tcagcaccac cccaatctcg gcaaagtgca
    34621 gaatagattc ggcatcggtc accagtcgca gcccccacgg gccaataatg cagccggcga
    34681 tcaggtagcc aagtaccgat cccagaccaa gacgtaccgc aatgggtaca atcagcgccg
    34741 ccgatccgag gtagatcagc gcctgaacca gcgtatgact atccatgatg ggcctcctgc
    34801 cattccagca gacgttgctt atagtggcgc gcctgccctt cgagggtttc gtcgtcacaa
    34861 ataaaggtgc agtgcatggc aaacggtggc agccagttca gcccgcagta gattgccgtc
    34921 gcctgtagcg gctgcgacag cacatcaaag cccggatgcg caccaatttc aaaatggctt
    34981 tccccgccgc cggtcgtcac cgcccacagc aaatgtttgc catgcagcgc cgtgccgccg
    35041 tgaccgtaag cccagccgtg cgacaaaact ttatcgatcc aaagtttaag aagcggagga
    35101 atgctgtacc actgcatcgg gtgctgccag acgatcagat cggcgcgaga gagcgcctcc
    35161 tgctcggcgg caatatcgat attgaagtca ggatagagtt gataaagaga gcgaatttcg
    35221 acgccttcca gcgtccttgc ctgttcaagc atccgtttat tcgcatggga atgatgcgga
    35281 tacggatgcg cataaatgat gaggatcata gttattagcc cgcgtgttta cgcctttgtt
    35341 ttcccacaaa gtgtagtcat atattcacgc ggcttatagt caataatatt gattcagaaa
    35401 agagagaaat ctgaacaaat aatcccattc tctgcccgcg gatcgtcata ttgctggtct
    35461 gcgccagtga cattcctcga gtctccgggg caaacgctac ggaaatcaac aagccaaaca
    35521 gcgagatacc cgcccccatt agcatcgtgt tactgatacc gtaattattg ataaatatcg
    35581 gcagtgccca ggtcgaaaca atggtgccaa tacgacttaa ggacataatc acgcccacgg
    35641 cagaggcgcg gatatccgtc gggaagagtt cattaggata gagccactgc aaattacccg
    35701 gcccgccaga gaaaaaggca tacaccgcaa aggccatcac taccagccag atccccatat
    35761 ccgggattag ccccaaaacc gccaacgcca gcgtcatcat ggcaaagctg ccaatcaaca
    35821 atggacgccg tccggcagtg tttagccaca gcatcggcgg aatacagccg agcataaaga
    35881 acaggctaat caccacattc cctagtgccg cgtttttgcc aacccccaat cccaacgaac
    35941 caacgatttg cgggccaaag gtgtaaatgg cgaacattgg gatcacctgg caggtccaga
    36001 tggcggcaac aaacagaaca aaaggaaaat ggcggcgatt aaacagatcg cgaaaacggg
    36061 tttgctgcgg ctgctcttca tcgaaagcca ccggttcgcc aaacagtttt atcatcatct
    36121 cttcgcactc ttttactcgc cctttgcgta ataaccagcg gggagattct ggcagttcga
    36181 atcgaccaat caaaatcaac aaacaaggga tcgccgcgct acccagcatc cagcgccaac
    36241 caccttccac atcataaagc cagtagccga ccagatcggc gcaggtcgcg ccgacatacc
    36301 acatcgcggc aataaagctg atggaaaacg cccgctgacg ggtactggag aactcggtga
    36361 tcatcgaagt ggcgatggga taatctgcac cgatgacaat gccgatgagt acccgcatca
    36421 ccaacagttc gacgggggat gaaacacaca tcgtcgccac cgatatcacg ccgatggcga
    36481 tgatatcaat gaggaacatt ttgcgccgtc cgactttatc ggagatatag ccaaacagcg
    36541 atgtgccaac gaacagcccg gcgagcgttc ccgcgcccag caagccaatc cagtcagcgt
    36601 ccagtttcag cgccggagtc agttgctcca gcgccacgcc aatcattacc agtacataac
    36661 catccagaaa cggaccaccg cttccccaca gcaaaatgcg gcggtgaata gaggagaatt
    36721 tgagatcgtc aaagtttctg gacggttgca tgggttattc ctgttttttt gcaatgagag
    36781 tcagtacggc gttacgtatc aaaccaacat cagccgtaac ggaactccac accaaaggtg
    36841 ccgcgcgggt attcccactg ttccagcgcc gaccccagcc ccagaatgcg acaggtgccg
    36901 cactccagac atccggcgta atcgaagcgc acactgccgt catcctgctt cttgtacaga
    36961 cctgcggggc acgctttcac cagcagctcc agcacctgtt tatcagcatc ggccttcaca
    37021 acaatgtgcg gatgctcttc atcgacattg aatttattga cgcccagttt gacgtccaca
    37081 ttgacgggag aagtcataaa acggtcactc ctttcatgcc atccttgatc agattgatga
    37141 agcccacttt cttgccgtgg cggaggattt tcttgcgcat cagttccggc gcactgccgt
    37201 caatggtgaa caggtcacgc gccacgccca ccgccagttc cgggttgccg ctaaacatgc
    37261 gtgggttatc aaggaacgcc ggtagtttct ggtacatgcg catatcgcgc agcgggccac
    37321 tctccagatg ctgacgatat tccgcgagtt tttgcttact gaaatcgtcg cttttcatcg
    37381 ctgaaagcac ggtttttgct gcagcttccc cggcggcaat cgccagatcc ataccgcgaa
    37441 tggtaaaacc gaggttcata cacattccgg cggcatcacc ggcaatcaat acgccgtcac
    37501 cgaccagctc cggcagcatg ttgatgcctg cttccggcac tacgtgagcg gaatattcca
    37561 ccagcttgcc gcccgcgatc agcggtgcaa cggccggatg ctgtttgaaa tcttccagca
    37621 tttgcggcac cgattttttc gcgtcatgca gatgatgcag accacaaacc agccccagcg
    37681 acagggtatt ttcattggta taaaggaagc cgccgcccat caggccatcg gtgggtgatc
    37741 ccgcaaacag acaagccgcg ccctgattac cctgcaactg aaaacggtct tcgattaccg
    37801 acttcggtaa ctcgatcagt tccttcacgc caaccgccac atccgtcggt ttaacgcgtt
    37861 ttgccatccc caacttttcg gcaaggatgg agttcacccc atcagcaagg agcaccgttt
    37921 tcgcttcaat cacatcacca tcggcttcta caccgacgac tttgccatcg cgctgtacga
    37981 ggttatcgac gcggatcccg gtaattaact gcgcgcccgc ttcttcggcc tgctccatca
    38041 accaggcatc aaatttactg cgcaaaacgg agtaagaacg ctgggatggc gaggtttcgt
    38101 caccgttgca gtagtccata gtcatcgctg acttttccgt cataaacgcg agtttttcat
    38161 gggtgatcag gcgttctacg ggggcggaat cggcgaaacc tggaataatg tgttccagac
    38221 tgtgggcata gagacgcccg ccagtaacgt tcttggcacc tgcggaattg ccacgctcga
    38281 taactaacac ttgcgcccct tcgcgggcga gcaccagtgc ggcaaccgaa ccggcaagcc
    38341 ctgcaccgac gatgatggcg tcaaagatat cttcggacat aagagctccc tgtcagcggc
    38401 agagccgtaa tgcagccctg ccagagtgga tcaacgcgct aaagctgcgg tcagtgccgg
    38461 aaggatcttc acggcatcgc caacaatgcc gtaatccgcg tactggaaga tcggcgcatt
    38521 tttatcttta ttgatggcga aaatggtttg cgacgcgtta gcgccaacca tgtgctggat
    38581 ctacccggag atccccaccg ccaggtacag ttcaggtttc agcatcaggt tggagatacc
    38641 gacatagcgt tcgtgctcca tccatttttc gttttctgcc accggacgag aacaggccaa
    38701 ctccgcacct atcgccttgc aaagctgttc tgccagcgca atgttctctt tgctgccaat
    38761 gccgcgaccg acgctgacca ccagacgggc tttgtcgaga tcgacgctgt tgctctggcg
    38821 cgcctgggtt gccgtgcggg taatcgccac agccggagcc tgccactcca cggtgtgcgt
    38881 ttcgccagtg cgtgacgcgt ctggctgagc cgcatcgaac gtgccgctgc tgatggtcag
    38941 taccgcatac ggcgtggcaa tgcgttcttc gccaatcgcc agaccgccgt aaaccatgtg
    39001 tttcactgtt gctttaccgt cctgtacgct gacggtgctg gcatcgttag acaccgccgc
    39061 tttaaggcga taacccagtt ttgccgccag taatttgccg cgacgggtgt ttggcaacag
    39121 caccagaccg tcagcgccgt gctggcgaat agtgtcagcc atgacaccgg cgtaatcttc
    39181 gatcatccga tcgtccggtt tgccgtttaa tttccagaca tgattagcgc cgagctggat
    39241 tgcctgtgcg ccgtcggcat cattgaggac aaaggcattg atttgattag ctaaagcctg
    39301 cgcaccgttc atcagttccg gcagacgaga gggggtatcg ctgaataccc agacttgaga
    39361 aaacgtgttc atagcatccc ctgtaattaa atgactttgc gaagattttc agcaaatgcg
    39421 gcgatctgtt cttcgccgtc gccttcaatc acgatgcgct gacgctcgcg ctgtttcggc
    39481 gcggcaacct gttgttcaga ccaggcgtcc acggcgttaa aaccaatatc cgccgccgac
    39541 catacctgga ctggcttttt cgccgcgccg agaatggctt tcatcgaagg aatttgtggg
    39601 gagttgatat cagtggaaac agcaacaacc gcaggcagcg gaatgcttaa ggtttcggtt
    39661 tcatcttcca gttcgcgctc aacggtgagg gtatctgccg tcagggagat aattttgctg
    39721 acgccgttaa ctgccggaat attgaggatt tcgcccacca gcagaccaac ctgctgggca
    39781 taaaggtcgg aagaaccatc gccacagagg atcagatcaa agcctgcttt ctgggcggct
    39841 gcagccagtg cgctcgccgt ttgttgcggc agtgcctgct cgaactggtc atcaatcacc
    39901 acaatcagtt catccgggcc gcgcgatagc acatctttac gccctttggc gttggtcagg
    39961 gctttaccgc ccacacttaa ggctgtcacc tgcgcctctg ctgcctgttg ctttagctgg
    40021 caagccgctt caatagcgtt gagatcgtat tggcttattt tggcatcggc tttgctgaag
    40081 tctaatgaac catcagcatt attgaccgca atatcctgtt catcaggcac gcacttatag
    40141 caagtaataa tcttcattgc atctccagaa atcatgaagg aaacgtcgtt catatcggtg
    40201 gtgttaatta cagatatcac gccctttaat ttggaaataa aactttaatc accaatattg
    40261 aaagtcagat gctatttttt tacccacaaa agaatataac aaactgatta ataaccaaaa
    40321 ttaacgaaaa acgccctctg ttcactcgaa acaaaaatgt gataccaatc a
//
LOCUS       DAZPAB010000015        38775 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZPAB010000015 DAZPAB010000000
VERSION     DAZPAB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.015"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..155
                     /locus_tag="ACHUPG_000718"
     CDS             <1..155
                     /locus_tag="ACHUPG_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0860449.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /gene="uidR"
                     /locus_tag="ACHUPG_000719"
     CDS             384..974
                     /gene="uidR"
                     /locus_tag="ACHUPG_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR0860450.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3065
                     /gene="uidA"
                     /locus_tag="ACHUPG_000720"
     CDS             1362..3065
                     /gene="uidA"
                     /locus_tag="ACHUPG_000720"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR0860451.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="ACHUPG_000721"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="ACHUPG_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="ACHUPG_000722"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="ACHUPG_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR0860452.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="ACHUPG_000723"
     CDS             complement(5626..7134)
                     /locus_tag="ACHUPG_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR0860453.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="ACHUPG_000724"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="ACHUPG_000724"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR0860454.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /gene="fumB"
                     /locus_tag="ACHUPG_000725"
     CDS             8609..10255
                     /gene="fumB"
                     /locus_tag="ACHUPG_000725"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR0860455.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="ACHUPG_000726"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="ACHUPG_000726"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR0860456.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="ACHUPG_000727"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="ACHUPG_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR0860457.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="ACHUPG_000728"
     CDS             complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="ACHUPG_000728"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR0860458.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14283..15602)
                     /locus_tag="ACHUPG_000729"
     CDS             complement(14283..15602)
                     /locus_tag="ACHUPG_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HFR0860459.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="ACHUPG_000730"
     CDS             complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="ACHUPG_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR0860460.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16699..17034
                     /locus_tag="ACHUPG_000731"
     CDS             16699..17034
                     /locus_tag="ACHUPG_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR0860461.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17031..17753)
                     /gene="folM"
                     /locus_tag="ACHUPG_000732"
     CDS             complement(17031..17753)
                     /gene="folM"
                     /locus_tag="ACHUPG_000732"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR0860462.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17790..19172)
                     /locus_tag="ACHUPG_000733"
     CDS             complement(17790..19172)
                     /locus_tag="ACHUPG_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0860463.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19358..20302)
                     /gene="ydgH"
                     /locus_tag="ACHUPG_000734"
     CDS             complement(19358..20302)
                     /gene="ydgH"
                     /locus_tag="ACHUPG_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR0860464.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20826..22358
                     /gene="pntA"
                     /locus_tag="ACHUPG_000735"
     CDS             20826..22358
                     /gene="pntA"
                     /locus_tag="ACHUPG_000735"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR0860465.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22369..23757
                     /gene="pntB"
                     /locus_tag="ACHUPG_000736"
     CDS             22369..23757
                     /gene="pntB"
                     /locus_tag="ACHUPG_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR0860466.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="ACHUPG_000737"
     CDS             complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="ACHUPG_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR0860467.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25227..25592
                     /gene="mdtJ"
                     /locus_tag="ACHUPG_000738"
     CDS             25227..25592
                     /gene="mdtJ"
                     /locus_tag="ACHUPG_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR0860468.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25579..25908
                     /gene="mdtI"
                     /locus_tag="ACHUPG_000739"
     CDS             25579..25908
                     /gene="mdtI"
                     /locus_tag="ACHUPG_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR0860469.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25947..26768)
                     /locus_tag="ACHUPG_000740"
     CDS             complement(25947..26768)
                     /locus_tag="ACHUPG_000740"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR0860470.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26868..26951)
                     /locus_tag="ACHUPG_000741"
     CDS             complement(26868..26951)
                     /locus_tag="ACHUPG_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0860471.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27044..27352)
                     /gene="asr"
                     /locus_tag="ACHUPG_000742"
     CDS             complement(27044..27352)
                     /gene="asr"
                     /locus_tag="ACHUPG_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR0860472.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQPAKPAAQPAA
                     "
     gene            27623..27727
                     /locus_tag="ACHUPG_000743"
     CDS             27623..27727
                     /locus_tag="ACHUPG_000743"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR0860473.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(27776..29029)
                     /locus_tag="ACHUPG_000744"
                     /pseudo
     CDS             complement(27776..29029)
                     /locus_tag="ACHUPG_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29136..30038
                     /locus_tag="ACHUPG_000745"
     CDS             29136..30038
                     /locus_tag="ACHUPG_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0860474.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAKEPFVFFDPHVGTGLYDDILGLMRR
                     YHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWL
                     VWPKHHEQSPAARNFRIHLLNALR"
     gene            30173..31393
                     /gene="mlc"
                     /locus_tag="ACHUPG_000746"
                     /gene_synonym="dgsA"
     CDS             30173..31393
                     /gene="mlc"
                     /locus_tag="ACHUPG_000746"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR0860475.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31518..32213
                     /gene="bioD"
                     /locus_tag="ACHUPG_000747"
     CDS             31518..32213
                     /gene="bioD"
                     /locus_tag="ACHUPG_000747"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR0860476.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32166..33458)
                     /gene="clcB"
                     /locus_tag="ACHUPG_000748"
     CDS             complement(32166..33458)
                     /gene="clcB"
                     /locus_tag="ACHUPG_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFR0860477.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33617..34231)
                     /gene="dmsD"
                     /locus_tag="ACHUPG_000749"
     CDS             complement(33617..34231)
                     /gene="dmsD"
                     /locus_tag="ACHUPG_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR0860478.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34274..35128)
                     /gene="ynfH"
                     /locus_tag="ACHUPG_000750"
     CDS             complement(34274..35128)
                     /gene="ynfH"
                     /locus_tag="ACHUPG_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR0860479.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35130..35747)
                     /gene="ynfG"
                     /locus_tag="ACHUPG_000751"
     CDS             complement(35130..35747)
                     /gene="ynfG"
                     /locus_tag="ACHUPG_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFR0860480.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35758..36593)
                     /locus_tag="ACHUPG_000752"
                     /pseudo
     CDS             complement(35758..36593)
                     /locus_tag="ACHUPG_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
     gene            complement(36654..37688)
                     /locus_tag="ACHUPG_000753"
                     /pseudo
     CDS             complement(36654..37688)
                     /locus_tag="ACHUPG_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
     gene            37746..>38775
                     /locus_tag="ACHUPG_000754"
     CDS             37746..>38775
                     /locus_tag="ACHUPG_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR0860481.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRD
                     SMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVE"
BASE COUNT         9902 a         9391 c         9926 g         9556 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaac actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaggcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggtatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctag
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtga
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgtcag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    13261 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    13321 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg
    13381 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    13441 cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg
    13501 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    13561 gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    13621 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    13681 tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa
    13741 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    13801 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    13861 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    13921 agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    13981 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca
    14041 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt
    14101 tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt
    14161 aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc
    14221 cccggtgtat tcatattcag taaattcaat tttccaccgg ggcttgttta ttatgctaag
    14281 gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct
    14341 gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg
    14401 atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg
    14461 acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc
    14521 gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt
    14581 ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg
    14641 ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat
    14701 gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt
    14761 acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc
    14821 gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa
    14881 ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca
    14941 taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg
    15001 atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca
    15061 ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc
    15121 agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca
    15181 gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca
    15241 tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac
    15301 aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta
    15361 ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta
    15421 acataaccgt taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga
    15481 atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg
    15541 gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc
    15601 acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa
    15661 tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat
    15721 agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct
    15781 tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt
    15841 gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    15901 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    15961 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    16021 cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg
    16081 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta
    16141 acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca
    16201 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    16261 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    16321 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    16381 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    16441 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    16501 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    16561 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    16621 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    16681 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    16741 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    16801 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg cattgaagcc
    16861 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    16921 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    16981 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    17041 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa
    17101 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    17161 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    17221 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    17281 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    17341 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    17401 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    17461 agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga
    17521 ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    17581 tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc
    17641 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    17701 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc
    17761 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    17821 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    17881 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat
    17941 aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg
    18001 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    18061 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    18121 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg
    18181 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    18241 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    18301 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    18361 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa
    18421 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    18481 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    18541 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt
    18601 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    18661 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    18721 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    18781 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    18841 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    18901 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    18961 ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    19021 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    19081 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    19141 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    19201 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    19261 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    19321 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    19381 cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt
    19441 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    19501 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    19561 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    19621 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    19681 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    19741 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    19801 tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac
    19861 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    19921 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    19981 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    20041 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    20101 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    20161 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    20221 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    20281 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    20341 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    20401 atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt
    20461 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    20521 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    20581 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    20641 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    20701 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    20761 gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    20821 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    20881 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    20941 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    21001 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    21061 aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    21121 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    21181 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    21241 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg
    21301 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg
    21361 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    21421 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    21481 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    21541 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    21601 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    21661 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg
    21721 aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    21781 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    21841 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    21901 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    21961 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    22021 cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg
    22081 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    22141 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg
    22201 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta
    22261 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    22321 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    22381 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    22441 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    22501 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    22561 gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca
    22621 gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    22681 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    22741 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc
    22801 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    22861 ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc
    22921 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    22981 ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac
    23041 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    23101 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    23161 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    23221 gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    23281 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    23341 cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc
    23401 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    23461 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    23521 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    23581 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    23641 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    23701 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    23761 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    23821 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    23881 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    23941 tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    24001 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    24061 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    24121 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    24181 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    24241 actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    24301 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    24361 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    24421 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg
    24481 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    24541 ccgcgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    24601 gatgattgtc atcaaaatcg acactgccag tacacgcggc acacgccagc ggaccatatg
    24661 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    24721 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    24781 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt
    24841 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    24901 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    24961 ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat
    25021 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    25081 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
    25141 tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
    25201 aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
    25261 ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg
    25321 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
    25381 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
    25441 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
    25501 taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
    25561 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
    25621 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
    25681 aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag
    25741 gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc
    25801 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
    25861 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc
    25921 ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
    25981 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
    26041 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
    26101 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
    26161 acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga
    26221 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
    26281 aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac
    26341 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
    26401 gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac
    26461 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
    26521 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
    26581 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
    26641 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
    26701 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
    26761 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
    26821 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
    26881 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
    26941 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
    27001 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
    27061 cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg
    27121 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
    27181 gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg
    27241 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
    27301 cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac
    27361 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
    27421 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
    27481 taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct accaataata
    27541 gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca
    27601 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    27661 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    27721 ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg
    27781 cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    27841 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    27901 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc
    27961 gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg
    28021 agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac
    28081 caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca
    28141 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    28201 ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca
    28261 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    28321 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa
    28381 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    28441 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
    28501 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
    28561 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
    28621 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    28681 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    28741 catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca
    28801 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
    28861 gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga
    28921 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    28981 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    29041 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct
    29101 aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc
    29161 gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata
    29221 tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac
    29281 tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag
    29341 atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc
    29401 agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg
    29461 tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg
    29521 aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc
    29581 tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta
    29641 tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac
    29701 tggctgaact ggcgaaagaa ccctttgtct tttttgatcc gcacgtcggg actgggctgt
    29761 atgacgatat tctcggactg atgcgacgtt accatttgac gcccgtcatc actcaggagg
    29821 tgggcgaggc aatgaccatc atcggtctgg tttccgccgg tctgggtgtt tcaattttgc
    29881 ctgcgtcatt taaacgtgtt cagctcaacg aaatgcgctg ggtgccgatt gctgaagagg
    29941 atgcggtttc tgaaatgtgg ttggtctggc cgaaacatca tgaacaaagt ccagctgcgc
    30001 gtaactttcg tattcatctg ctgaatgctc tcaggtgagg gaaatttcag cgaaaaagcc
    30061 cgaaaaatgt gctgttaatc acatgcctaa gtaaaaattt gacgacacgt attgaagtgc
    30121 ttcaccatag cctacagatt atttcggagc gcgaaaatat agggagtatg cggtggttgc
    30181 tgaaaaccag cctgggcaca ttgatcaaat aaagcagacc aacgcgggcg cggtttatcg
    30241 cctgattgat cagcttggtc cagtctcgcg tatcgatctt tcccgtctgg cgcagctggc
    30301 tcctgccagt atcactaaaa ttgtccgtga gatgctcgaa gcacacctgg tgcgagagct
    30361 ggaaatcaaa gaagcgggga accgtggccg tccggcggtg gggctggtgg ttgaaactga
    30421 agcctggcac tatctttctc tgcgcattag tcgcggggag attttccttg ctctgcgcga
    30481 tctgagcagc aaactggtgg tggaagagtc gcaggaactg gcgttaaaag atgacttgcc
    30541 attgctggat cgtatcattt cccatatcga tcagtttttt atccgccacc agaaaaaact
    30601 tgagcgtcta acttcgattg ccataaccct gccgggaatt attgatacgg aaaatggtat
    30661 tgtacatcgc atgccgttct acgaggatgt aaaagagatg ccgctcggcg aggcgctgga
    30721 gcagcatacc ggcgtaccgg tttatattca gcatgatatc agcgcatgga cgatggcaga
    30781 ggccttgttt ggtgcctcac gcggggcgcg cgatgtgatt caggtggtta tcgatcacaa
    30841 cgtgggggtg ggcgtcatta ccgatggtca tctgctacac gcaggcagca gtagtctcgt
    30901 ggaaataggc cacacacagg tcgacccata tggtaaacgc tgttattgcg ggaaccacgg
    30961 ctgcctcgaa accatcgcca gtgtggacag tattcttgag ctggcacagc tgcgtctcaa
    31021 tcaatccatg agctcgatgt tacatgggca accgttaacc gtggactcat tgtgtcaggc
    31081 ggcattgcgc ggcgatctac tggcaaaaga catcattatc ggggtgggcg cgcatgtagg
    31141 gcgcattctt gccatcatgg tgaatttatt taacccacaa aaaatactga ttggctcacc
    31201 gttaagtaaa gcggcagata tcctcttccc ggtcatctca gacagcatcc gtcagcaggc
    31261 ccttcctgcg tatagtcagc acattagcgt tgagagtact cagttttcta accagggcac
    31321 gatggcaggc gctgcactgg taaaagacgc gatgtataac ggttctttgt tgattcgtct
    31381 gttgcagggt taacattttt taactgttct accaaaattt gcgctatctc aatttgggcc
    31441 aggaaagcat aacttagact ttcaaggtta attattttcc tagtttatat ttgtgaagca
    31501 taacggtgga gttagtgatg ctgaagcgtt tctttattac cggtacagac acttctgtag
    31561 ggaaaacggt ggtttcccgc gcattgctac aagcgttagc ctcccaggga aaaagggttg
    31621 cgggatataa acccgtagcg aaggggagca aagagacacc cgaagggctg cgtaataaag
    31681 atgccttggt gttgcagagt gtttcaacca tcgaactgcc ttatgaagca gttaatccta
    31741 tcgcgttaag cgaagaagaa agtagcgtgg cgcacagttg cccaatcaat tacaccctca
    31801 tttcaaacgg cctggcaaac ctgaccgata aagtcgatca tgtcgtggta gaagggactg
    31861 gcggctggcg cagtctgatg aatgatttgc gtccactctc tgaatgggta gtgcaggaac
    31921 aactgccggt gttgatggtt gtcggtattc aggaaggttg cattaaccat gcactgctaa
    31981 cagctcaggc gatcgccaac gacgggctgc cgctcattgg ctgggtggct aaccgaatca
    32041 acccaggact ggcgcattat gcggaaatca ttgatgtgct gggtaaaaaa cttccggcac
    32101 cgctcattgg tgaactgcct tatctgccgc gcgccgaaca gcgtgaactg gggcaataca
    32161 tccgcttagc tatgctgcgc agtgtgctgg cggtagatag agtcacggtg taacgtccgc
    32221 gaaattaccg acgcaattac gcaggcaatc aataaaccgg ggagtagctg atactccccg
    32281 gtcatttcac atatcatcaa cgtcgacata atcggcgcgt gcgtggttgc cgccaacagt
    32341 gtcgccatcc cggtcaatcc gagtaaaagc gtaatttctt cgccatcagg gaaccataat
    32401 cccaggctac gaccatacaa catgccaatg gcaagaccga taaatagcgt cggtgtaaag
    32461 acaccaccgg gtgcgccgga accgctactc gccagcacgg cacacagttt acaaaggaag
    32521 atcccggcaa tgatcattaa cagtggtggg gcggttaaaa aggattgtac ggtgctatag
    32581 ccgttgcccc acactgcagg tgtgaacagg gaaagcagac ccacgatcaa cccgcctagt
    32641 gccagttgcc agggcggcgc aagtttgaga ctcacaaatc cacgatgaca ggcgttcatt
    32701 aacgttaaca acagtggtcc gcacagacct gccagcacac ctgtactgat aatcagcgca
    32761 tagtcacgag cctgaaccgt cactgagagt tgtacgttgt agagtaacgc gtcgctatga
    32821 ttaatcagat tgctaaccag ccatgccacg atggcggaaa taatcaccgg gccgagagag
    32881 gccaacatca tagtgccaaa cagcacttcg gctataaata aactgccagc aagcggggca
    32941 cgataggccg cagccattcc cgccgcggcc ccacaggcga tccataattt ccattcctgg
    33001 cgtggcgtaa aacgttgggc aaaacaggag gcggcaaggg cagctaaaag aatcatcgca
    33061 ccttcgcgac caattgcact gccgctggtt actaccagca gcgaggcaag cgatttaacc
    33121 aggcttgctg cgtaatcgaa ctgtccatcg gtttgcaacg cttccatgta atcggtcggc
    33181 gcatgagggc gttgttgggt aaatttctgc cagcccatca gcaacaaacc cgccgccagt
    33241 ccgccgagcg ccggagttag cagccgtcgc caggaggaaa ggtttgtcgc tgcattgacc
    33301 agactgccgg agtcattatt gaggaacaac cactccagta gcagcatcgc atgacgaaac
    33361 ccggcaacgg caaagacagc gagaataccg acgactgttg cgataagcag acggcggaac
    33421 atagtgcgca gatccggata gatgtgtaga tgatgcatga gcagaaatgg gcaatgaaac
    33481 acgggcgact attttgcggc aaaagaggcc aaccgcaaaa gcagtgcgtg ctttaagaga
    33541 taaaaaaacg tgacattgta acgtattatt gccgaatcgt gcgtaatcgt caccatccgg
    33601 caatattacg gtgatcctat cgaaagagtg gtttaaccgc gacaggaatc aacagttgtg
    33661 attgccattg cgccagcgtt aagcgagcca gttcacccag tgcacggtag aaagggtgtt
    33721 ctgctttttc gataaaaaca tcaagaaaac gtgttgacca cggaaaaagg tgccatgcca
    33781 gcaattcttc gcattctgtc tggcgaccat tctccgccaa ccacgcagcc atcaacagca
    33841 gtgaaccaaa atgatcttcc ggttcgtttt gcttcatttc aaactgaatg cctttctcgc
    33901 gcatccactg acgaagtgcc aatgttgaat cgccaaacag cacagattcg cgatccagcc
    33961 agaccgaccc ccacggcgga gacggcagtg cccacgggcc gacaaacaaa cgctgccagg
    34021 cctgggcgtg agtctcttca cactgtgtct gaaaagccgt caccagcggc gctaatgacg
    34081 cttctggtag aggccactga gtttcccagc cgtcactggt gagtaccgca accagaggtg
    34141 cggcttccgc actctctgga gcgtaataaa acagcgcgcc caacacgcgc gccgcgacag
    34201 aaaaattatc ttgctgtgaa aaatgggtca ttcctaacat cctgaaatgt gcgggttgcc
    34261 ccgcaccaat ttgttaacct gcaatagcca ttcctactgt catgtgcagg ccataaaaga
    34321 gcacacgccc gatcatctca ccaacaagta tgaggattag ccccagaaca agtccggcaa
    34381 cgtgaggttc acggcgacga atgagcggac aaagccagca tccaagacct gcgcataaca
    34441 ataccacacg ccagacctgt aaggaggcgt aatctggtac cagtgcgctg gcttgttgca
    34501 cggatgagtg aatagacgcc agagacaaac cttgcagcac aatcacctcg gcacaagcga
    34561 tcagcgccag aacgctaatg atggcaaatg gtgtggtatt aaatgtaacg cgggctgccc
    34621 gcagaattgc ggcagcaagt attggaccgc tcaacaatac cgtcaggaag aaagccagcg
    34681 tagtgtaacc gttatgccag gttggcacgg tgtcgatttg atacacgcag gtcatcatcc
    34741 agacgaaaat gacgccgagc gccatgctga caagtaacca gagtttcccc aacgcttgcg
    34801 gcattttacc gatgaccgcc accagccacc atagtccgcc aacggcaaaa aatatggaac
    34861 cggcagcaat ctcgttactc agaccagaag ctccgattcg attcagcgag ttaaacgctc
    34921 gcagtggcga tcccaggtgc atgacagagg caataaaccc gatgcccatc aacagccaga
    34981 ggaaaaacat gccacgaaca atgtgctgac ggtctgcgtc atttttggcg gcgaaccagc
    35041 caattccgct aacaatcagc gcgcctacta cgcattgacc caatacggta aagataacca
    35101 gtggccattc atgccatcca tttcccattt tacacttcct ccggattagc cagataacca
    35161 gtggtatcgc cggtcgggcg gctgttggcg ttaggtttga taacgatatt gggttttgtg
    35221 aaatgcgcgc gcggcagtgg cgcgacggca gccagagtgc cgtgcttctg gcgcagctct
    35281 tcaattggac cgaactcaag tgcacgcagc gggcaggatt cgacgcatat gggttgtttc
    35341 ccctcggcga cgcgcgaata acaaccatcg cacttcgtca tgtgcccttt ttcagcattg
    35401 tactgtggtg cgccgtacgg acaagccatg tggcagtagc ggcagccgat acaaacatct
    35461 tcatcaacca ccacaaagcc atcttcacgc ttatgcattg cgccgctggg gcagactttt
    35521 gtacatgcgg ggtcatcgca atggttacag gagatggaga gataataagc gaacacgttc
    35581 tggtgccaga tgccattatc ttcctgccag tcgccgccag catattcata aatacggcgg
    35641 aaactgactt ccgggccaag atctttaaaa tctttgcacg ccagttcgca ggttttacag
    35701 ccagtacagc ggctggaatc gataaaaaat ccatattgtg tggtcatggg ctactcctta
    35761 aaccttttcg atctggacaa gattgctgtg cgacgggttt ccctttgcca gcggtgaagg
    35821 gcggtgagag gtcagaatat tgatactgcc gccgtgatcg acccggtcac caaacatatc
    35881 cgctttaagc cacgcacctt gcccgatggc ggtaacgcca ggcagaatac gcggagtcac
    35941 ttttgcggca atcagcattt ctccattatt gttaaatacc cgcacggtat cgccatgacg
    36001 gataccgcgt gcctgagcat caatggggtt gatccacacc tcttgtgggc aggcctgctg
    36061 taacacatca atattgccgt agctggagtg ggtactacgc ctgttccgta caggacatgt
    36121 acagcctcat tgccgtaatc tttcaggatc cgccgaagat tatcactgat ggtatccagg
    36181 gcttcgtccc aacttatccg ttcaaattta ccttcaccgc gctcgccgac gcgcttcatg
    36241 ggatatttca acctgtcagg atgattcatc cgtcggcgga tagagcgccc gcgtaaacat
    36301 gcccgaacct gatgattacc gtaaacgtcg tcacctgtcg tatcagactc cacccagtac
    36361 acggtgtcat ctttcacatg caaacgtagc agacagcggc tcccgcagtt aacggtgcag
    36421 gaactccaga ccgttttctc ttctaccgga gcctctgccg cccggaccat ttgggaaaat
    36481 ggcagagtga aagcactgct tgccagcgcg agactgccaa gtgcggaggt tttcatcaga
    36541 cttctatggc tgatttcagc cttcatgagc gcctctgtgg tatggatttt catcattact
    36601 cacttattgc ttttcaaaca aaatgtcatg ccagaattta tggttgtagt gggttatatt
    36661 ttttcgatct cgaccagatt agtgtgctgc gggtttccct tcgccagtgg tgaagggcgc
    36721 agagtggtta gcgtattcac acatccgcca tggtcgattt tatcgccaga catattgggc
    36781 tcgtgccagg ctccctggcc catagcgcta actccaggga gaatgcgtgg tgttactttg
    36841 gctggtagcc gaacttcgcc acgatggtta aacacccgca ccatatcgcc gttggcaatc
    36901 ccacgtttct gtgcatctat agggttgatc cacacctcct gacggcaggc tgccttcagg
    36961 acatcaatgt tgccgtaggt cgagtgagta cgggatttgt aatggaaacc aaacagttgc
    37021 agtgggaagg ttctacgttc aggggagtcc cagccttcaa aggttgaggc ataaactggc
    37081 agtgggctta tcacttcatc tttttccagt tcccaggtac gggcaatttc cgccagcttg
    37141 ctggaataaa tttcaatctt accggaaggc gttttaagtg gatttgcctc ggggtcgtca
    37201 cgaaatgctt tgtaggcgac aaaatggcca ttgggatctt tacgcttata gatacccatt
    37261 tttttcagtt cgtcgtaaga cggtaacgcc ggatctttgg caagcatttt ggcgtacaga
    37321 tgttgtaacc attgttcctg cgtgcgacct tctgtgaact tttgatagac gtcaggtcca
    37381 agacgtttcg cgacttcact caggatccag taaatcggtt tacgttcgaa tttttcgctg
    37441 gtgactggct ggaggaaaat gagatatccc atgttaccgg cgtagtcgtt aggaataata
    37501 tcttcctgct caacggtcat caggtcttcc agcagaatgt cggcatattt tgccgatgag
    37561 gtcataaagt tttcgatgac cacaatcatt tcgcatttcg attcgtcctg cagaatttca
    37621 tgcgttttgt tgatgtcaga atgctgatta acgagggtat ttcccgcgta gttccagatg
    37681 gactccattg ccagacggat ggatggactt gaagtagttg actcaatgct acttaaggtc
    37741 ctgatatgac tgacattaat catgataaca ctgtatatcc atccagttct ttctctgggt
    37801 ctccctatcg gtatcagcaa gtctacacct caagccctga cacaaggctg acacaaatca
    37861 atctactcag cgtagctaag cctcagttag tccgcagagc caatggtcga tatactatcc
    37921 gcttcagact caagggacag accacaccgt ttctttcagt atcgacccgc agcacagaca
    37981 ggagagtagc cacaatgcgt cagagagagc ttgcagccac cgctaaggcc ttcatgttgg
    38041 ataggcctga agtctcacta caggagctta cagagcatct ccggtctatg gctgagcagt
    38101 tcctgacaga cgctagtgac gattactgga atggacttga ggtcgctact ctggtcgacg
    38161 agaagtccaa cctgaaggag ctagccgcta cgcaagcctt gagtctggac cagcagaagg
    38221 gcataaggtt agcgctggaa gtcctgaccg cagcacagca gagagtcgac actggtgata
    38281 cctctggtct gattaagctg attgatgata ataaccttac tgatgactca actataggcg
    38341 actctacgtc aattctgaat aatgagcagg gggatagacc ggcagttttc acacaggagc
    38401 gccagtcatc tgtagttttt tcaagcctag tgtcctcgct gctggctgag aaggtccaga
    38461 ccctgaagac atccagctac aaggacctct cgtcctcact caatactgtc tcccggttcc
    38521 tgcctgaaga catggacctg atgtcccgct ctgggtggct ggctgtcagg gactctatgc
    38581 tggcttctga ggtgagacca tcgacaatca acaagctgct gaccaaagcg aagatgtgtc
    38641 tcgattatgg cctgatgaat gggcaacttg agggccgcaa ccctattgaa cggatgaagc
    38701 tgaccaagga tatcgactcc aagcgcagag cgttcacaga cgaggagctg gagagacttc
    38761 tggtccgtgt tgagt
//
LOCUS       DAZPAB010000016        38363 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZPAB010000016 DAZPAB010000000
VERSION     DAZPAB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.016"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            55..131
                     /locus_tag="ACHUPG_000755"
     tRNA            55..131
                     /locus_tag="ACHUPG_000755"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:89..91,aa:Met,seq:cat)
     gene            180..254
                     /locus_tag="ACHUPG_000756"
     tRNA            180..254
                     /locus_tag="ACHUPG_000756"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:212..214,aa:Gln,seq:ctg)
     gene            292..366
                     /locus_tag="ACHUPG_000757"
     tRNA            292..366
                     /locus_tag="ACHUPG_000757"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:324..326,aa:Gln,seq:ctg)
     gene            complement(520..1695)
                     /gene="ubiF"
                     /locus_tag="ACHUPG_000758"
     CDS             complement(520..1695)
                     /gene="ubiF"
                     /locus_tag="ACHUPG_000758"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR0860482.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1841..3265
                     /gene="miaB"
                     /locus_tag="ACHUPG_000759"
     CDS             1841..3265
                     /gene="miaB"
                     /locus_tag="ACHUPG_000759"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR0860483.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3418..4458
                     /locus_tag="ACHUPG_000760"
     CDS             3418..4458
                     /locus_tag="ACHUPG_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR0860484.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4455..4922
                     /gene="ybeY"
                     /locus_tag="ACHUPG_000761"
     CDS             4455..4922
                     /gene="ybeY"
                     /locus_tag="ACHUPG_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR0860485.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            5012..5890
                     /gene="corC"
                     /locus_tag="ACHUPG_000762"
     CDS             5012..5890
                     /gene="corC"
                     /locus_tag="ACHUPG_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR0860486.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5915..7453
                     /gene="lnt"
                     /locus_tag="ACHUPG_000763"
     CDS             5915..7453
                     /gene="lnt"
                     /locus_tag="ACHUPG_000763"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR0860487.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            7850..8758
                     /gene="gltI"
                     /locus_tag="ACHUPG_000764"
     CDS             7850..8758
                     /gene="gltI"
                     /locus_tag="ACHUPG_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR0860488.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8928..9668
                     /gene="gltJ"
                     /locus_tag="ACHUPG_000765"
     CDS             8928..9668
                     /gene="gltJ"
                     /locus_tag="ACHUPG_000765"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR0860489.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            9668..10342
                     /gene="gltK"
                     /locus_tag="ACHUPG_000766"
     CDS             9668..10342
                     /gene="gltK"
                     /locus_tag="ACHUPG_000766"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR0860490.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            10342..11067
                     /gene="gltL"
                     /locus_tag="ACHUPG_000767"
     CDS             10342..11067
                     /gene="gltL"
                     /locus_tag="ACHUPG_000767"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR0860491.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            11185..12120
                     /gene="rihA"
                     /locus_tag="ACHUPG_000768"
     CDS             11185..12120
                     /gene="rihA"
                     /locus_tag="ACHUPG_000768"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR0860492.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            12204..13874
                     /gene="hscC"
                     /locus_tag="ACHUPG_000769"
                     /pseudo
     CDS             12204..13874
                     /gene="hscC"
                     /locus_tag="ACHUPG_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(13934..15385)
                     /gene="djlC"
                     /locus_tag="ACHUPG_000770"
     CDS             complement(13934..15385)
                     /gene="djlC"
                     /locus_tag="ACHUPG_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR0860493.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(15382..16089)
                     /locus_tag="ACHUPG_000771"
     CDS             complement(15382..16089)
                     /locus_tag="ACHUPG_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR0860494.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            16191..16745
                     /locus_tag="ACHUPG_000772"
     CDS             16191..16745
                     /locus_tag="ACHUPG_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0860495.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(16755..18182)
                     /locus_tag="ACHUPG_000773"
     CDS             complement(16755..18182)
                     /locus_tag="ACHUPG_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR0860496.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(18179..18886)
                     /locus_tag="ACHUPG_000774"
     CDS             complement(18179..18886)
                     /locus_tag="ACHUPG_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR0860497.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            19050..20027
                     /gene="ybeQ"
                     /locus_tag="ACHUPG_000775"
     CDS             19050..20027
                     /gene="ybeQ"
                     /locus_tag="ACHUPG_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFR0860498.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(20097..20579)
                     /locus_tag="ACHUPG_000776"
     CDS             complement(20097..20579)
                     /locus_tag="ACHUPG_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR0860499.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            20814..23396
                     /gene="leuS"
                     /locus_tag="ACHUPG_000777"
     CDS             20814..23396
                     /gene="leuS"
                     /locus_tag="ACHUPG_000777"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR0860500.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            23411..23992
                     /gene="lptE"
                     /locus_tag="ACHUPG_000778"
     CDS             23411..23992
                     /gene="lptE"
                     /locus_tag="ACHUPG_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR0860501.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            23992..25023
                     /gene="holA"
                     /locus_tag="ACHUPG_000779"
     CDS             23992..25023
                     /gene="holA"
                     /locus_tag="ACHUPG_000779"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR0860502.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            25025..25666
                     /gene="nadD"
                     /locus_tag="ACHUPG_000780"
     CDS             25025..25666
                     /gene="nadD"
                     /locus_tag="ACHUPG_000780"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR0860503.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            25690..26301
                     /locus_tag="ACHUPG_000781"
     CDS             25690..26301
                     /locus_tag="ACHUPG_000781"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFR0860504.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            26561..26878
                     /gene="rsfS"
                     /locus_tag="ACHUPG_000782"
     CDS             26561..26878
                     /gene="rsfS"
                     /locus_tag="ACHUPG_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR0860505.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            26882..27349
                     /gene="rlmH"
                     /locus_tag="ACHUPG_000783"
     CDS             26882..27349
                     /gene="rlmH"
                     /locus_tag="ACHUPG_000783"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR0860506.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            27380..29281
                     /gene="mrdA"
                     /locus_tag="ACHUPG_000784"
     CDS             27380..29281
                     /gene="mrdA"
                     /locus_tag="ACHUPG_000784"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR0860507.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            29284..30396
                     /gene="mrdB"
                     /locus_tag="ACHUPG_000785"
                     /gene_synonym="rodA"
     CDS             29284..30396
                     /gene="mrdB"
                     /locus_tag="ACHUPG_000785"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR0860508.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            30407..31495
                     /gene="rlpA"
                     /locus_tag="ACHUPG_000786"
     CDS             30407..31495
                     /gene="rlpA"
                     /locus_tag="ACHUPG_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR0860509.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            31634..32845
                     /gene="dacA"
                     /locus_tag="ACHUPG_000787"
     CDS             31634..32845
                     /gene="dacA"
                     /locus_tag="ACHUPG_000787"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR0860510.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            32956..33219
                     /gene="ybeD"
                     /locus_tag="ACHUPG_000788"
     CDS             32956..33219
                     /gene="ybeD"
                     /locus_tag="ACHUPG_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR0860511.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            33320..33961
                     /gene="lipB"
                     /locus_tag="ACHUPG_000789"
     CDS             33320..33961
                     /gene="lipB"
                     /locus_tag="ACHUPG_000789"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR0860512.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            34220..35173
                     /locus_tag="ACHUPG_000790"
     CDS             34220..35173
                     /locus_tag="ACHUPG_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR0860513.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            35382..36347
                     /gene="lipA"
                     /locus_tag="ACHUPG_000791"
     CDS             35382..36347
                     /gene="lipA"
                     /locus_tag="ACHUPG_000791"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR0860514.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(36448..36651)
                     /gene="tatE"
                     /locus_tag="ACHUPG_000792"
     CDS             complement(36448..36651)
                     /gene="tatE"
                     /locus_tag="ACHUPG_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR0860515.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(36780..37568)
                     /locus_tag="ACHUPG_000793"
     CDS             complement(36780..37568)
                     /locus_tag="ACHUPG_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR0860516.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            37661..38044
                     /gene="crcB"
                     /locus_tag="ACHUPG_000794"
     CDS             37661..38044
                     /gene="crcB"
                     /locus_tag="ACHUPG_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR0860517.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(38098..38307)
                     /gene="cspE"
                     /locus_tag="ACHUPG_000795"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(38098..38307)
                     /gene="cspE"
                     /locus_tag="ACHUPG_000795"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR0860518.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
BASE COUNT         9613 a         9772 c         9634 g         9344 t
ORIGIN      
        1 taccggcatt ccctggttcg aatccaggta ccccagccat cgaagaaata atctggctac
       61 gtagctcagt tggttagagc acatcactca taatgatggg gtcacaggtt cgaatcccgt
      121 cgtagccacc aaattctgaa tgtgtcgaat acgttcggca aattcaaaaa ccaatttgtt
      181 ggggtatcgc caagcggtaa ggcaccggat tctgattccg gcattccgag gttcgaatcc
      241 tcgtacccca gccaatttat tcaagacgct tacatcgtaa gtgcacccag ttggggtatc
      301 gccaagcggt aaggcaccgg attctgattc cggcattccg aggttcgaat cctcgtaccc
      361 cagccacatt aaaaaagctc gcttcggcga gctttttgct tttctgcgtt cattcaatgt
      421 cgaatgcgat gttgacacgt cttatccttc aatgtcggat gcgacgctgc cgcgtcttat
      481 ccgacctacg gctggcacgc atccggcaat gttgtaaggc tacaacccta acgcatattt
      541 cagcgcctga cgtttcaaca cgccagcacg ctctgccgcc attaacccga gattacgcac
      601 aaaacgcagc ggcggcagat tattactgaa tccggcataa aacagatcca taccactttg
      661 cataatgaag ttatccgcca tgcgccgcat ctggtaacgc ttgaggacag gataactggc
      721 ccacgcttca ccgtagctgc gggcattcac cagaacatca atcagggcat cgacatcacg
      781 ataaccaaga ttcaccccct gccccgccag cggatggatg gtatgcgcgg catcgcccac
      841 cagcgcaagc cccggctgca catactgcaa cgcatgtcgg cgcgtcagcg gaaacgcacc
      901 agcggcaagc ggtgtaacgt aacccagacg cgacgggaaa tgcttcgcga tttccgcctg
      961 gagttgcgcc atattcatat tctgcaactg gcgaatacgc gtcggagagt cataccacac
     1021 cagcgatgcc cagttatcaa acaacggcag aaacgcacgc ggtccgtccg gggtaaattg
     1081 ctgccaggtg ctgtcgccgg gatcgttctc acactgtacg ctaatcaaca tgcacgactg
     1141 cgcatactgc caggcatgaa cgccaattcc cgccatctgc cgcacctgag aatttgcgcc
     1201 gtcggcacca atcaccagtt tcgcgcggat cgtttcaccg cccttcagtt ccagctcctg
     1261 aagatcatta tctcgatgta atgcaatcag cgaggttggc acacgtaacg ttactttcgg
     1321 atgcgcttcc agcgcctgcc acaacgcctg ttgcagcact gtgttttcca ccatgtagcc
     1381 aagcaacggc aactttaatt cggctgcgtc aaataccaca tgtgccgttt cccactccca
     1441 ggtttccagt ctgcggtaag gatggcaacg catagcctgt actgcatccc agacccctaa
     1501 ccctttaagc aatgataccg aagccgcgct gatcgccgag atccgcacgt ccggttggct
     1561 gtcagcgaca aacggtgcag gttctgcgtg ctcgatcacc gttaccgaaa atccgtgctg
     1621 tgccagcccc agcgccagtg cgccgccgac cattcctccg ccgacaatgg caatttccgt
     1681 tggttgattt gtcatggtca atcatcctgt tagcaatatg ctttaagttt acaggatttt
     1741 tacccccaag ggctgataac gctcatactg gtcacaacgg cagcaaagca ttacactatg
     1801 cgccctgcat tcctggctac tatttcgcaa gagcaagtcg atgaccaaaa aactccatat
     1861 taaaacctgg ggctgtcaga tgaacgagta cgattcatcg aagatggccg atctgctgga
     1921 tgccacccac ggctatcaac tgaccgacgt ggcggaagaa gcggatgtgc tactgctgaa
     1981 cacctgctca atccgcgaga aggctcagga aaaagtcttc catcagttgg gtcgctggaa
     2041 actgttaaaa gagaagaatc cagacctgat tatcggcgtc ggtggctgcg tggcatcgca
     2101 agaaggcgag cacattcgcc agcgcgccca ctatgtcgat attatttttg ggccgcaaac
     2161 gctgcaccgt ctgccggaga tgatcaactc cgtgcgcggc gaccgcagcc cggttgtaga
     2221 tatcagcttc ccggaaatcg agaagtttga ccgtctgccg gaaccgcgcg ccgaagggcc
     2281 gaccgcgttt gtctccatca tggaaggctg caataaatat tgcacctact gcgtggtgcc
     2341 ttacacccgt ggtgaagagg taagccgtcc gtctgacgat attctgtttg agattgccca
     2401 gcttgcggct cagggcgtgc gtgaagtcaa cctgctcggt cagaatgtga atgcctggcg
     2461 tggtgagaac tacgacggca ccaccggatc gtttgccgat ctgttgcgtc tggttgctgc
     2521 gatcgacggg atcgatcgta ttcgctttac caccagccat ccgatcgaat tcaccgacga
     2581 tatcatcgaa gtgtaccgcg acacgcctga gctggtgagc ttcctgcatc tgccggtaca
     2641 gagcggttcc gatcgcattc tgaacctgat gggacgtacc catacggcgc tggagtacaa
     2701 agcgatcatc cgtaaactgc gtgcggcgcg tccggatatt cagatcagtt ctgacttcat
     2761 tgttggcttc cctggcgaaa ccaccgaaga cttcgagaaa acgatgaagc tgattgccga
     2821 cgtcaatttc gacatgagct acagctttat cttctctgca cgtccaggaa cgcctgccgc
     2881 cgatatggtt gatgatgttc cggaagaaga gaagaagcag cgtctgtata ttctgcaaga
     2941 acgtatcaat cagcaagcga tggcatggag ccgtcggatg ctcggcacca cccagcgtat
     3001 tctggtagaa gggacttcgc gtaagagcat catggagctt tccgggcgta ccgaaaataa
     3061 ccgcgtggtc aacttcgagg gcacaccgga tatgatcggt aaattcgtcg atgtagaaat
     3121 taccgacgtc tacccgaact ctctgcgcgg taaagtggtg cgaactgaag atgaaatggg
     3181 tctgcgcatg gcagaaacac cggagtcggt gattgcccgc acccgcaaag aaaacgacct
     3241 tggcgtgggt tattatcagc cgtaattctc aggcctgccg ttccggtggg ccttgttttt
     3301 tctctcttta tccccatctt tatcgcaatg cttgcgcctt tttcctgtgg cgtgaataat
     3361 ttccttatag gccggtgaat gttcagcttc cggcggcata cagataagag gaacggtttg
     3421 aacatagaca ctcgcgaaat caccctggag ccagcagaca atgcgcgtct gttgagcctg
     3481 tgcggcccgt ttgatgacaa catcaagcag ctcgaacgcc gtctcggcat cgagatcaat
     3541 cgccgcgata accactttaa actgaccggc cgtccgattt gcgtcaccgc tgcggcagac
     3601 attctgcgta gcctgtatgt cgatactgcc ccgatgcgcg gtcagattca ggatatcgaa
     3661 ccggaacaga tccaccttgc gattaaagaa gcgcgggtac tggagcaaag cgcggagagc
     3721 gtgccggagt acggcaaagc ggtcaatatc aaaactaaac gcggcgtaat taagccgcgc
     3781 acgccaaacc aggcgcagta catcgccaat attctcgacc atgacattac cttcggcgtt
     3841 ggcccggcgg gtacgggtaa aacctacctg gcagtggctg cggcagttga tgccctggag
     3901 cgtcaggaaa ttcgccgtat tctgctgact cgtccggcgg tcgaagccgg tgagaaactg
     3961 ggcttcctgc ctggcgattt aagccagaaa gtagacccgt atctgcgccc actgtacgac
     4021 gcgctgtttg aaatgctggg ctttgagaaa gtcgagaaac tgattgagcg caacgttatt
     4081 gaagtcgcgc cgctggccta tatgcgtggt cgtacgctga acgacgcgtt tatcattctc
     4141 gatgagagcc agaacactac catcgaacag atgaagatgt tcctgacccg tatcggtttt
     4201 aactcaaaag cggttatcac cggcgacgtc acacagatcg acctgccgcg taatactaaa
     4261 tcaggcttac gtcatgccat tgaagtgctg gccgatgtcg aagagatcag ctttaacttc
     4321 ttccacagcg aagacgtggt tcgtcacccg gtggtggcgc gtatcgttaa cgcttatgaa
     4381 gcctgggaag aagcagaaca aaaacgtaaa gcggcgctgg cagcagaacg caagcgcgaa
     4441 gaacaggaac aaaaatgagt caggtgatcc tcgatttaca actggcatgt gaagataatt
     4501 ccgggttacc ggaagagagc cagtttcaaa catggctgaa tgcggtgatc ccgcagtttc
     4561 aggaagagtc ggaagtgact attcgcgtgg tcgataccgc cgaaagccac agtctgaatc
     4621 tgacctatcg cggtaaggat aagccgacca acgtgctctc cttcccgttt gaagtgccgc
     4681 ctggcatgga gatgtcgcta ctgggcgatc tggttatctg ccgtcaggtg gttgagaagg
     4741 aagctcagga gcaaggcaaa ccactggagg cgcactgggc gcatatggtg gtgcacggca
     4801 gtctgcattt gttaggttac gatcacattg aagatgacga agcagaagaa atggaagccc
     4861 tcgaaacaga gattatgctt gctctgggct atgaggatcc gtacattgcc gagaaagaat
     4921 aatttgccag gcatttgact ggcagacgtc cccgccgttg gtgattaacg acggcgtttt
     4981 gattaactaa catgagaacc catacgacgc catgagcgac gacaattcac acagtagtga
     5041 cacgataagc aacaagaagg gatttttctc cctgttactc agccaacttt tccacggtga
     5101 accgaaaaac cgtgacgaac tgctggcgct gatccgtgat tccgggcaga acgaccttat
     5161 cgacgaagat acgcgcgata tgctcgaagg ggtgatggac atcgcagacc aacgcgttcg
     5221 cgacatcatg atcccccgct cccagatgat taccctgaaa cgcaaccaga cgctggacga
     5281 atgccttgat gtcatcatcg agtccgccca ctcacgtttc ccggtgatta gcgaagacaa
     5341 agatcacatt gaagggattc tgatggcgaa agacttgctg ccgtttatgc gcagcgatgc
     5401 tgaagccttc agcatggaca aagtgttacg tcaggcggtt gtcgttcctg aaagtaagcg
     5461 cgtagaccgg atgctgaaag agtttcgctc tcagcgttac cacatggcga tcgttattga
     5521 cgaattcggt ggggtttccg gtctggtgac cattgaagac atcctggaac tgattgttgg
     5581 tgagattgaa gacgagtatg acgaagaaga tgatatcgac ttccgtcagc tgagtcgtca
     5641 tacctggacc gtgcgcgcac tggcttccat tgaagacttc aacgaagcgt tcggcaccca
     5701 ctttagcgat gaagaagtcg acactatcgg tggtctggtg atgcaggcgt ttgggcatct
     5761 tccggcgcgt ggcgaaacta tcgacatcga cggttaccag ttcaaagtgg cgatggccga
     5821 tagtcggcgt attattcagg ttcatgtcaa aatcccggat gactcacccc agccaaagct
     5881 ggatgaataa aaccgaaact ggatagataa ctacatggat tttgcctcat taattgaacg
     5941 ccagcgcatt cgcctgctgc tggcgttatt attcggtgcc tgcggaacgc tggccttctc
     6001 tccttacgac gtctggcctg cggcgattat ttcgctgatg gggcttcagg cgttgacctt
     6061 taaccgtcgc ccactccagt ctgccgctat tggcttttgc tggggatttg gcctctttgg
     6121 cagcggtatt aactgggtct atgtcagcat cgcgaccttt ggcgggatgc ctggcccggt
     6181 taacatcttc ctggtggtgc tgctggcggc gtatttgtcg ctgtataccg gactgtttgc
     6241 tggcgtgctg tcgcgtctgt ggccgaaaac cacctggctg cgcgtggcga ttgccacccc
     6301 tgctctctgg caagtgaccg agtttctgcg cggttgggtg ctgactggct tcccgtggtt
     6361 acagttcggc tatagccaga ttgatggccc gttaaaaggg ctggcaccgc taatgggcgt
     6421 ggaagccatt aacttcctgc tgatgatggt tagtggcctg ctggcactgg cgttggtcaa
     6481 acgcaactgg cgtccgctgg tggtggccgt cgtgctgttt gccctaccct tcccgctgcg
     6541 ttacatccaa tggtttaccc cgcaaccgga gaaaaccatt caggtttcga tggttcaggg
     6601 cgatattccg caatcgctga aatgggacga aggccagctt cttaatacgc taaagattta
     6661 ctacaacgca acggcaccgc tgatgggcaa atcatcgttg attatctggc cggagtcggc
     6721 gataaccgat ctggaaatta atcagcaacc gttcctcaaa gcactggacg gtgagttgcg
     6781 tgataaaggt agctcgctgg taaccgggat tgtcgacgcg cgtctcaata agcagaaccg
     6841 ctacgatacc tacaacacca tcatcacgct gggtaaaggt gcaccgtaca gctacgaatc
     6901 agccgatcgc tataacaaaa accatctggt gccgtttggc gagtttgtcc cgctggagtc
     6961 gattctgcgt ccgttagcac cgttctttga tctgccgatg tcgtcgttca gccgtgggcc
     7021 atatatccag ccgccgctgt cggcaaatgg tattgagctt actgcggcta tttgctacga
     7081 gattattctc gccgagcaag tgcgcgataa cttccgcccg gataccgact acctgctgac
     7141 tatctccaac gatgcgtggt ttggtaaatc tattggtcca tggcaacact tccagatggc
     7201 gcgaatgcgt gcgctggagc tggcgcgccc actgttgcgc agcaccaaca acggcattac
     7261 ggcggtgatt ggcccgcagg gtgagattca ggcgatgatc ccgcagttcg cccgcgaggt
     7321 gttaaccact aacgtgacgc cgaccaccgg actcacacca tacgcacgta ccggcaactg
     7381 gccgctgtgg gtgctgacgg cactgtttgg ttttgctgct gtgttgatga gtctgcgtca
     7441 gcgacgtaaa taatcccttc ttatcgtgcc ggaatacatc ccatatttcg gcacgctcac
     7501 cggatgcggc atcagtgcat tatccggata acaatattct cctatccaac tttggcacat
     7561 ctattgcttt gttatacaag gcaaagcttg aaccagcatc agtacaaccc ggttgcacca
     7621 cagcaatcca ccggtagcac caaaagagtg cattttaaga attttgcttc agaatggtgc
     7681 ggtgcacctc gcaaaaacaa acaataaaac atcaatatct ttacatttag ccaaactaaa
     7741 tgttacctaa caggcacaac actgcacaat aaagttgcag acgataacaa cacaaacact
     7801 cacaacgggt atccatgcgt tcttaacgca gaagataaag gtgttggata tgcaattacg
     7861 taaacctgcc acagcaatcc tcgccctggc gctttccgca ggactggcac aggcagatga
     7921 cgccgccccg gcagcaggca gtacgctgga caaaatcgcc aaaaacggtg tgattgtcgt
     7981 cggtcaccgt gaatcttcag tacctttctc ttattacgac aatcagcaaa aagtggtggg
     8041 ttactcgcag gattactcca acgccattgt tgaagcagtg aaaaagaaac tcaacaaacc
     8101 ggacttgcag gtaaaactga ttccgattac ctcacaaaat cgtattccac tgctgcaaaa
     8161 cggcactttc gattttgaat gtggttctac caccaacaac gtcgaacgcc aaaaacaggc
     8221 ggctttctct gacactattt tcgtggtcgg tacgcgcctg ttgaccaaaa agggcggcga
     8281 tatcaaagat tttgccgacc tgaaaggcaa agccgtagtc gtcacttccg gcactacctc
     8341 tgaagttttg ctcaacaaac tgaatgaaga gcaaaaaatg aatatgcgca tcatcagcgc
     8401 caaagatcac ggtgactctt tccgcaccct ggaaagcggt cgtgccgttg cctttatgat
     8461 ggatgacgct ctgctggccg gtgaacgtgc gaaagcgaag aaaccagaca actgggatat
     8521 cgtcggcaag ccgcagtctc aggaagccta cggttgcatg ttgcgtaaag acgatccgca
     8581 gttcaaaaag ctgatggatg acactatcgc tcaggtgcag acctccggtg aagcggaaaa
     8641 atggtttgat aaatggttca aaaatccaat tccgccgaaa aacctgaaca tgaatttcga
     8701 actgtcagac gaaatgaaag cactgttcaa agaaccgaat gacaaggcac tgaactaatt
     8761 acaagaacca ggggcggaaa ttccagccct ctcgattgtt acgtagcacg gacagactat
     8821 acgcctgatg gtcgttcccc atcgggcctg aaaaccgcaa tacgctgggt aacaatcttc
     8881 gagggtagca gttaacgctg ctaccctctt ttttctggag tagatttatg tctatagact
     8941 ggaactgggg tattttttta caacaagccc cgttcggcaa taccacctat cttggttggg
     9001 tctggagtgg ttttcaggtg acgatcgctt tgtcgatctg cgcctggatt atcgctttcc
     9061 tcgtcggttc atttttcggc attttacgta ccgttccaaa ccgttttctc tccggtctgg
     9121 gtacgttgta tgtcgaactg ttccgcaacg tgccgttgat tgtgcaattc tttacctggt
     9181 atctggtgat cccggagctg ctgccggaga aaatcggcat gtggtttaag gccgagctgg
     9241 atcccaatat tcagtttttc ctttcatcca tgctctgcct ggggctgttt accgccgccc
     9301 gtgtttgcga acaggtacgc gccgctattc agtcgctacc gcgtgggcag aagaatgccg
     9361 cgctggcgat gggcctgacg ctaccgcaga cttatcgata cgtactgctg cctaatgctt
     9421 atcgcgttat cgtcccgccg atgacttccg agatgatgaa cctggtgaaa aactccgcca
     9481 tcgcctcgac catcggtctg gtggatatgg cggcgcaggc gggtaaattg ctcgactact
     9541 cggctcacgc ctgggagtcg tttaccgcca tcacgcttgc gtatgtgttg attaacgctt
     9601 ttatcatgct ggtgatgacg ctggttgaac gtaaagttcg cctgcccggc aacatggggg
     9661 gcaaataatg tacgagtttg actggagttc cattgttcct tccctgccat atctgctcga
     9721 cgggctggtg atcaccctga aaatcaccgt cacggcagtc gtgattggta ttttgtgggg
     9781 taccatgctg gcggtgatgc gcttgtccag cttcgcgcct gtttcgtggt ttgccaaagc
     9841 ctacgttaac gtattccgct cgattccttt agtcatggtt ttgctgtggt tttacttgat
     9901 tgtgccggga tttctgcaaa atgtgctggg attatcgcca aaaaatgata ttcgtttaat
     9961 ttcggcgatg gtggcgtttt cgatgtttga agcggcctac tattcagaga ttatccgtgc
    10021 cggtattcaa agtatttccc gtggtcagtc gagcgccgcg ctggcgttgg ggatgactca
    10081 ctggcagtcg atgaaactga ttattctgcc gcaggctttc cgcgcgatgg tgccgctgct
    10141 gctcactcag ggcatcgtac tgttccagga tacctcgctg gtgtatgtgt tatctctggc
    10201 cgatttcttc cgtaccgcct caaccattgg tgagcgtgat ggtacgcagg tcgagatgat
    10261 cctgtttgcc ggatttgttt atttcgttat tagccttagc gcgtcgttgt tggtcagcta
    10321 cttgaaaaga aggacagcat aatgattacc ctgaaaaatg tttcaaaatg gtatggtcac
    10381 tttcaggtgc tgaccgactg ctcaaccgaa gtgaaaaaag gcgaagtggt ggtggtttgc
    10441 ggcccgtctg gttccggcaa atcaacgctg attaaaaccg tcaacggcct cgaaccagtg
    10501 cagcaaggtg aaatcaccgt cgatggtatc gtggttaacg acaagaaaac cgatctggca
    10561 aagctgcgct cccgcgtcgg gatggtattc cagcatttcg agctgttccc tcatctgtcg
    10621 attatcgaaa acctgaccct ggcgcaggtg aaagtgctta aacgcgataa agcgcccgct
    10681 cgtgaaaaag cattgaaatt gctggagcgc gtgggacttt ccgcacatgc caataaattt
    10741 ccggcgcagc tttccggcgg tcagcagcag cgcgtggcaa tcgctcgcgc attgtgtatg
    10801 gctcctattg cgatgctgtt cgacgaaccg acatcggcgc tggatccgga gatgatcaac
    10861 gaagtgctgg acgtgatggt ggaactggcg aacgaaggaa tgaccatgat ggtggtgacc
    10921 cacgaaatgg gctttgcccg caaagtggcg aatcgggtga tctttatgga cgagggtaaa
    10981 attgtcgaag actcgccgaa agacgctttc ttcgatgatc cgaaatcgga ccgcgcaaaa
    11041 gacttcctcg cgaaaatcct gcattaatca ctctggcgca catacccaat tgcgcgccat
    11101 ctcacgcttt atcgtttcgc ccctcgtatc tgttacatcc tggcgttacc cttgtcgcaa
    11161 agaagcacac aacaaggagc aacaatggca ctgccaattc tgttagattg cgacccaggt
    11221 catgatgacg ctatcgcaat agttctcgca ctcgcctcac cagagcttga tgtcaaagca
    11281 attacgtctt ccgccggaaa ccagacacca gaaaaaacct tacgcaatgt tctgcgtatg
    11341 ctgaccttgc ttaatcgcac cgatattccg gtggcaggcg gcgcggtaaa accgttaatg
    11401 cgtgagttga ttatcgcgga caatgtgcac ggcgaaagcg gtctcgacgg cccggcatta
    11461 ccggaaccga cgttcgcacc gcaaaactgt acggcggtgg agctgatggc gaaaacgctg
    11521 cgcgaaagtg cggaacctgt cacgattgtt tctaccggac cgcaaactaa cgttgccttg
    11581 ctgctcaata gccatccgga actgcatagc aaaattgccc gtatcgtgat tatgggcggc
    11641 gcaatggggc ttggtaactg gacgcctgcg gctgaattta atatttacgt tgacccggaa
    11701 gcggcagaaa ttgtcttcca gtcagggatc ccggtggtga tggccggtct ggatgttact
    11761 cataaagcgc aaatccacgt tgaagacacc gagcgtttcc gcgcgattgg caaccctgtt
    11821 tcaaccattg ttgccgaact gctggatttc ttcctcgaat atcataaaga cgaaaaatgg
    11881 ggctttgtcg gcgcaccgct gcatgaccca tgcaccatcg cctggctgtt gaaacccgag
    11941 ttgtttacca ctgttgagcg ctgggttggc gtggaaacac aggggaaata tacccagggt
    12001 atgacggttg ttgattatta ttatctgact ggcaataaac cgaatgccac tgtaattgtc
    12061 gatgttgatc gtcagggctt tgttgattta ctggctgatc gtctgaaatt ttacgcttaa
    12121 cactcgtcat tgattaagat caccacgcgt tacctgggtg gtcttaatct atttatctct
    12181 ttttctttca agatataaat gcaatggata atgcagaact cgccataggt atcgatctcg
    12241 gcactaccaa tagtttaatt gccgtctgga aagacggtgc cgcgcaatta attccaaata
    12301 agttcggtga atatttaaca ccatccataa ttagcatgga tgaaaataat catattttag
    12361 tcggaaaacc ggctgtatca cggcgtactt cgcatccgga taaaacggca gcgttattta
    12421 aacgtgcaat gggcagtaat accaactggc ggttaggcag cgacacattt aacgcgccag
    12481 aactgtcctc tttggtatta cgctcattaa aagaagatgc cgaagaattt ctgcaacgtc
    12541 cgattaaaga tgtggtgatc tccgttctgg cttatttcag cgatgaacaa cgcaagcata
    12601 cccgtttagc agcggactta gccgggttaa atgcggtacg cttaattaat gaacccacag
    12661 cagctgcgat ggcgtatggc ctgcataccc aacaaaatac ccgttcgctg gtgtttgatc
    12721 tcggtggcgg cacgtttgac gttacggtgc ttgagtacgc cacgccggtg attgaagttc
    12781 acgcctccgc tggcgacaac ttcctcggtg gcgaagattt tacccatatg ttggtcgatg
    12841 acgttttaaa gcgcgcggat gtcacaagga ccacgcttaa cgagagtgaa ctggcagcct
    12901 tgtacgcctg tgtggaagcg gcaaaatgta gcaatcaatc gccaatgcac attcgctggt
    12961 agtaccagga tgaaacgcgg gaatgcgaat tttacgagaa cgaactggaa gatttgtggt
    13021 tgccgctgct caatcgcttg cgagtgccga ttgaacaggc gttgcgcgat gcgcgtctga
    13081 agccgagtca aatcgacagt ctggtgctgg ttggcggcgc gtcacaaatg ccgctggtgc
    13141 agcgaatcgc cgtgcgtctg tttggcaaat taccgtatca aagttacgat ccgagcacca
    13201 ttgtcgcgct gggcgcagca atccaggccg cctgccgctt acgcagtgaa gatattgaag
    13261 aagtgatcct cactgatatt tgcccttact cgttgggcgt tgaggttaac cgccagggcg
    13321 tttccggcat tttctcgccg attattgaac gaaacaccac tgtgcccgtg tcgcgtgtag
    13381 aaacttattc aaccatgcac ccggaacagg attcaattac ggttaacgtc tatcagggag
    13441 aaaaccacaa agttaaaaac aacattctgg tggaatcctt cgatgtgccg ttgaagaaaa
    13501 ccggggctta tcagtcgatt gatattcgct ttagttatga tatcaacggg ttgcttgaag
    13561 ttgacgtgct tctggaagac ggcagcgtta agtccagagt gattaaccac agcccggtaa
    13621 cattgagcgc gcagcagatt gaagagagtc ggacgcggtt atccgcattg aaaatttatc
    13681 cgcgcgatat gctcatcaat cgcaccttta aagccaaact ggaagagttg tgggcgcggg
    13741 cgctgggtga cgagcgagaa gagattggcc gggtgatcac cgattttgat gcggcgctgc
    13801 agtcaaacga tatggcccgc gtcgatgagg tcaggcggcg ggcgagcgtt tatttagcca
    13861 ttgagacctc ataatcacgg ctgccggatg cggcgtaaat gctgcatccg gcatcggcgc
    13921 aaaaaacaca tcgtcaacta aacaacatcg cgattttgcc gagagcataa cctataacca
    13981 gcaccgttaa actgattttg ataatgctgg taatgctctg tggtttttca ccctcatcat
    14041 ttttttcttc atggttttca ggtgttggct catcgacatc ttcatcctcc ggctcgagtt
    14101 ttactttcgt caacagcatc cgggtgataa tggcctctaa tggatgagca tctccgcctt
    14161 ccagccttgt attaagacgg gccagcatct cttgtttcgg aaacgcttca agtactacct
    14221 gttccgctac ccgatacaaa tccagtaacg gtgaatcttc ttctgtctcg ctaccgactc
    14281 tcgatttcca ccacgcccat gctttgcgtt tgaagaataa cgactcaata aacgattgac
    14341 ctaacggcga taattttccg ctatgtaaaa tttccgccca taacagccgc gtccgggcgc
    14401 tctcaccagg aacatatttc agcggttgac cttgcgcatc taccatgctc cgcgcttcga
    14461 taaccatcac taataacgcg aaataatccg ggcaatgacg cttgctccac tgcaacaaca
    14521 aattatcagc ttgtgtagat gggtaacttt cccagtacgc gccgagatca gcaagcaggc
    14581 tttccccctc gccgcagtac aaacgttgtg cacactggta ataccgggca tcttcgcttt
    14641 ctggttcttc cgcttgccac tgtttggcta caggaatgag ttccaccata cctgtttgca
    14701 ccgagctgta ccagcgcaac agtttgcgat gtaactttgc atcatcagga atgaaccacg
    14761 gtccttccat cgccaaaaac gcagtgacat agtcaggatg gtaacggcag caacgttcca
    14821 gcgcgaagta atattcaaca gtctggttct gtaacgcgac gggatattcc agaaggctaa
    14881 ggaaatcgaa acaatcacca gcctgaatgg cctcaagaaa agcctccctt tcttcctcat
    14941 ccattccttc gctgtcatta aacgaatgcc agttcaaacg ctctgccaac aaagaagcac
    15001 aatttaatga cagatatcgc gcttctatag cgattgcaca cagcggccag cgtaattgat
    15061 cgacatcctc catgttccag gtatttaatt gctgaataaa tttctgccag gcggagggct
    15121 gaaaacgatc actttctgaa tccagtaatg tcctgaatgc acgtagtatt tcatgttcag
    15181 cggtggcatc tttttcttca tcatctgctt cctcgacagg atttaccgcc agtcgcagcg
    15241 cctcttcata ggcctggcgt aatgctttaa acccctgcgg atcggtttcg ggatgacaca
    15301 acggtaagcg agcaagataa gcctggcgga taatgtctat ttgcgtcgtg ctttcaattt
    15361 cgaggatttg ccaacatgtt ttcataggtc gctcacatcc ttaagcgtta ccgggcattc
    15421 aggctggaca gttaatggct gccagggaag cgtggcatag aaacggtcgc ttcccgctgc
    15481 cagctcttcg aacatccgac tcccggcgct atcgtatttg tacagggtta gcgcctcgcg
    15541 tagcgaagcg ttgtcttcgc actgagaaga ttgccagtag agacgcccta gtgaataggc
    15601 agaaaaatag tgcatccagc tgtgatagta atggtgagct cgtgcataga cgcgcgattg
    15661 cagccataaa ctctcttctt ccgtcagcca gttgttgagc acaccggccc gtgacagaaa
    15721 tcccattcga acataatccc aggctttgat tcccccttct ccacagcaca tagcggtttg
    15781 ggcaacgaat tgggtataaa tcaaccctct ctccgagcca ccagcaatta atgctttcca
    15841 ctcttcaggg gaagagcgtt gccatgtcag atataagcca gctaaatcat tggcatgacc
    15901 atcatcagtc atctgataaa tagtctgaat taaatcatca cgattattta ttccccagga
    15961 tgatgagaga tcgaattctt ttatcccggt atacattttt ggcgagtcat gcgcgggttt
    16021 gcaactctga ttaagtattt ccatgggcgc agaaagcgca aaaagaagat attgttcttc
    16081 cttattcatt ttttaataat cccttaagtg cattaactga ctactttttt attattgatg
    16141 agtataaact tgacgctttc aaaataaaaa gaaaatcgaa gcattcacac atgaataaaa
    16201 aattaatgta tatattcgca atttttatag ttgcagcaat tacctgtatt agccaaccca
    16261 agaaaacgat gttgcgtgat aaagccatgg tgaattatgc ctttgattat ttaagatcac
    16321 caggcagtct accgttaacc acggcagcca cggagctttc cgcgattcat ggtcactcaa
    16381 catcgcaata tcgccttggc gaattttatc ttcatggtag cgacggtaaa ccactggatt
    16441 atacacaggc gagatactgg tatgagcaat cagcagaaca ggaaaatccg cgcgcacaaa
    16501 gcaaactggg gtggatctac ctgaaaggcc tgggagtcaa acccgatacc cgtaaagcaa
    16561 ttctctggta taaggaagca gctgaacaag ggtatgctca tgctcaatat actttgggtt
    16621 tgatctacag aaatggcact ggtattaatg ttaaccatta tgaatctcaa aaatggttaa
    16681 aactggccgc caaacaacat tacaaaaatg cggaaagatt acttgccgga cttcccgcac
    16741 attaacgtcg actgtcaccc gaagagatgg agtattttcc ctatcaaacc ggcaaagata
    16801 aaaatgtaga aaataatttt gatgacgccc agacagccca gcccgccatc atcaacgggc
    16861 attggctgcg gttgcggctc cggctcgacg gtttcctgac cggtaaaatt cgccgcctga
    16921 attaataatc gacgaataat taatgcttcc agtggacaac gatcttctgc atcaaggcgg
    16981 ctcacaatcg gcattaatga ttcttctgtc ggttggctgg cctgcactaa ttgtttggca
    17041 actaaatata atggccataa gggatgttct gtatcacgcg tgagtgagat ccgcggctta
    17101 cgtcctttgt gcaaaaccat ctcgaccagt gaacgcccca gcgcagagag tttttcatcg
    17161 ctgagtattt cgtataaatg aaaacgcgta cgggcgctgt cgccggggac ataaagtagc
    17221 ggttttccct gatcgttaac cagatcacgc gcttcaatca tcatcaccac taatgggtaa
    17281 taatagaccc gatgctgacg gcaccattgc aacattaaag catccgcctg ggtggaggga
    17341 tactcgcgcc agtagtaaca aagttctgcc agcaggcttt cgccttctcc acaatagacg
    17401 cgttgtgcgt attcataata acgcgcatct tcattatcag gttcagcttc ccgccacgac
    17461 cgggcgtaat ccaggagttc ggggattccc aggcggcttg cgctatacca gcggagtaaa
    17521 tttctgtgca gttttgcatc atcggggata atcactgcgc gatgttgcgc taaatacaat
    17581 gtcagccatt ctggatgcga tgaccaaatc cgagcgaaca tttggtaaaa atcgatggtg
    17641 tcattttgca cggcaactgg cagatgcaga atggtctgga agttaaaaac attccccttc
    17701 gcaatggcat ataaaaagga ttccagttct tcttcgtcta tttccccgac gttttcctcc
    17761 tgcaaccacc gcaatctttc tgctaataac accacgcact cgaaggataa atgggcagtg
    17821 ttcatggcga ttgtgcacag cgaccagcgt aattcatcaa tctcttccat cgagcaataa
    17881 tttaattgct gaatgaatcg ctgccaggcg gagggcagaa aacgttcgct atcagaggca
    17941 agtaacgcgc gaaaggcgag cagaatttca tgttctgcta cctcatattc ttctggttgc
    18001 caaacagatt tagcaggcga ctgcgcaatc cgtagcgcct cctcatacgc ctgacgaagt
    18061 tgtttaaaac cctgcggatc ggtttctgga tgaaaggacg gtaacagcgc cagataagcg
    18121 cggcggataa tatcgacgtc agtcgtttcc tctatatcga ggatcttcca gcaatttttc
    18181 ataagtcgct catatcctta agcgtttccg ggcatgtggg gtagtcagcc agtggttgcc
    18241 aggacaaggt agggtaatac gcatcatcac tggcaaataa ttgataaaac atatcattgc
    18301 cggaatcgtc gtattcttta cgcgctaaga attcgcgtaa taaaggcaga tgatcatctt
    18361 cgggagactg ccaatattgc cgaccaaagg tataaccggc aaagtattgc cgccagttac
    18421 tataataacg tagagcccgt agatgaatac gcgactggat ccatagactt tcttcttcac
    18481 tcaaccagtt attaagtacg cccattcgac ttaaaaaacc catccggaca taatcccatg
    18541 ctttaattcc tccctcacca cagcattcgg ctgtgctggc gacaaattgc gcataagcct
    18601 gaccttgctc atttaattca gcaagatagt cacgccattc acatggagaa taacgaaacc
    18661 agcggtgata aaaccctgcc aaccgagcgg catgaccgtt ctcgatcatt cggtgaatta
    18721 tgcgcagcag gtcatcacgg tcatcaattc cccatgagga agaaaggtca aaaaagcatg
    18781 tacctaaata cattttgggg gatgaatgcg aaggtaaaca gcattcattg cgtatttcca
    18841 tcggagtgga aagtgcaaac aatatttttt gcgaccccat gtccatataa tttatcctta
    18901 aacattcatt taatgaatgt aaactacatt tcgaggctca gttatttata cttttctgac
    18961 gcaacataat acgcataaaa taaggcttac gcaaaaatgg aacacataaa aatataatat
    19021 attttatcta caaccataag gaaatgataa tgatttttac gtcaagttgc tgcgataatt
    19081 tatcaataga tgagattatc gaacgtgctg aaaaaggcga ttgcgaggct caatatattg
    19141 ttgggtttta ttataatcgc gatagcgcaa ttgactctcc ggacgacgaa aaggcctttt
    19201 actggctgaa gctggccgct gagcaaggtc attgtgaagc acagtattcc ttagggcaaa
    19261 agtataccga ggataaaagc cgtcataaag ataatgagca agccatcttc tggctgaaaa
    19321 aagctgccct acaaggccat actttcgctt ccaacgccct tggctggata ctggatcgtg
    19381 gagaagaccc caactataaa gaagcggtta cctggtatca gatagccgcg gagagcggaa
    19441 tgtcttatgc gcaaaataat cttggatgga tgtacagaaa tggcaacgga gtcgcacaag
    19501 actatacgct ggcatttttc tggtacaaac aagctgcatt acaaggccat agttacgcac
    19561 aagacaatct ggccgatctt tataaagacg gagaaggcgt tgctcaaaac aagacactcg
    19621 ccgcattctg gtatttgaaa agcgcacagc agggtaatcg gcacgcccag tttcaaattg
    19681 catgggatta taacgctggc gaaggggtgg accaggacta taagcaagcg atgtactggt
    19741 atctgaaggc tgccgctcag gaaagcgtcg gcgcttacgt caacatcggt tatatgtata
    19801 aacacggaca aggcgttgag aaagattatc aggctgcctt tgaatggttt acgaaagccg
    19861 ctgaatgcaa tgacgccact gcctggtata acctggccat tatgtatcac tacggagaag
    19921 gaagacctgt cgatctccga caggctctcg acctgtatcg taaagttcag tcatccggaa
    19981 ccagagatgt cagtcaggaa attcgtgaga ctgaagcttt actgtagaaa taaaaacaag
    20041 ggccggggaa tttcattctg cccatattct ctttattgct gtgcgaaatc agactattac
    20101 ggctcaaacg ggcgtctctg gaactggtca taaccacatt tcgggcatag cgtcagcact
    20161 tccggtgtgt agatcgggag atggaagtga catttctcgc agaccagatt tcccagcccg
    20221 accacttctc cgctgtgata aaccccatga tgattgaggt cctggaaaat ttcgcgccat
    20281 tcaagctgcg ttttatcggt gatgtctgcc agctcctgcc acaagctttc tttaatcacc
    20341 cgcataaaga cgctgtcaga ttcttctttc aggctctctt catagctcat ggcgaactct
    20401 tccaggtcac gtctgacagc tcgcgtcagc tcatcgatct cggttcgcgt taactcccct
    20461 gtttttatta cgcgctcgcg cgcctgttcc accagtgcgt cgatatcacg ttcgccattg
    20521 cgcaggcgtt cgttcagtga cgcgaccagt tcacggtaat attgagcaac cttgttcatc
    20581 gtttcatctc ccgggtcgtt aactgactat aataatagac gttctttgtt ccttgctttg
    20641 ctaatacgcc cacaaactct gtaaattaat gtacgaataa tccgggcatc atttcaacgt
    20701 cgagtgcgcg aaaggctgtt ttgacgcgga cgtttgggct atgctatgcg gatctgaaaa
    20761 accacctcaa cgctacattt gtagccgtat tgaaaacagg accactggct gccatgcaag
    20821 agcaataccg cccggaagag atagaatcca aagtacagct tcattgggat gagaagcgca
    20881 catttgaagt aaccgaagac gagagcaaag agaagtatta ctgcctgtct atgcttccct
    20941 atccttctgg tcgactacac atgggccacg tacgtaacta caccatcggt gacgtgatcg
    21001 cccgttacca gcgcatgctg ggcaaaaacg ttctgcagcc gatcggctgg gacgcgtttg
    21061 gtctgcctgc ggaaggcgcg gcggtgaaaa acaacaccgc tccggcaccg tggacgtacg
    21121 acaacatcgc gtatatgaaa aaccagctca aaatgctggg ctttggttat gactggagcc
    21181 gcgagctggc aacctgtacg ccggaatact accgttggga acagaaattc ttcaccgagc
    21241 tgtataaaaa aggcctggta tataaaaaga cttctgcggt caactggtgc ccgaacgacc
    21301 agaccgtact ggcgaacgaa caagttatcg acggctgctg ctggcgctgc gataccaaag
    21361 ttgaacgtaa agagatcccg cagtggttta tcaaaatcac tgcttacgct gacgagctgc
    21421 tcaacgatct ggataaactg gatcactggc cagacaccgt taaaaccatg cagcgtaact
    21481 ggatcggtcg ttccgaaggc gtggagatca ccttcaacgt taacgactat gacaacacgc
    21541 tgaccgttta cactacccgc ccggacacct ttatgggttg tacctacctg gcggtagctg
    21601 cgggtcatcc gctggcgcag aaagcggcgg aaaataatcc tgaactggcg gcctttattg
    21661 acgaatgccg taacaccaaa gttgccgaag ctgaaatggc gacgatggag aaaaaaggcg
    21721 tcgatactgg ctttaaagct gttcacccat taacgggcga agaaattccc gtttgggcag
    21781 caaacttcgt attgatggag tacggcacgg gcgcagttat ggcggttccg ggtcacgacc
    21841 agcgcgacta cgagtttgcc tctaaatacg gcctgaacat caaaccggtt atcctggcgg
    21901 ctgacggctc tgagccagat ctttctcagc aagccctgac tgaaaaaggc gtgctgttca
    21961 actctggcga gttcaacggt cttgaccatg aagcggcctt caacgccatc gccgataaac
    22021 tgactgcgat gggcgttggc gagcgtaaag tgaactaccg cctgcgcgac tggggtgttt
    22081 ctcgccagcg ttactggggc gcgccgattc cgatggtgac gctggaagac ggtaccgtaa
    22141 tgccaacccc ggacgaccag ctgccggtga tcctgccgga agatgtggtg atggacggca
    22201 ttaccagccc gattaaagca gatccggagt gggcaaaaac taccgttaac ggtatgccag
    22261 cgctgcgtga aaccgacact ttcgacacct ttatggagtc ctcctggtac tatgcgcgct
    22321 acacttgccc agagtacaaa gaaggtatgc tggattccga agcggctaac tactggctgc
    22381 cggtggatat ctacattggt ggtatcgaac acgccattat gcacctgctc tacttccgct
    22441 tcttccacaa actgatgcgt gatgcaggca tggtgaactc tgacgaacca gcgaaacagt
    22501 tgctgtgtca gggtatggtg ctggcagatg ccttctacta tgttggcgaa aacggcgaac
    22561 gtaactgggt ttccccggtt gatgctatcg ttgaacgtga cgagaaaggc cgtatcgtga
    22621 aagcgaaaga tgcggcaggc catgaactgg tttataccgg catgagcaaa atgtccaagt
    22681 cgaagaacaa cggtatcgac ccgcaggtga tggttgaacg ttacggcgcg gacaccgttc
    22741 gtctgtttat gatgtttgct tctccggctg atatgactct cgaatggcag gaatccggtg
    22801 tggaaggggc taaccgcttc ctgaaacgtg tctggaaact ggtgtacgag cacacagcaa
    22861 aaggtgatgt tgcggcactg aacgttgatg cgctgactga agatcagaaa gcgctgcgtc
    22921 gcgatgtgca taaaactatc gctaaagtga ccgatgatat cggccgtcgt cagaccttca
    22981 acaccgcaat tgcggcgatt atggagctga tgaacaaact ggcgaaagca ccgaccgatg
    23041 gcgagcagga tcgcgctctg atgcaggaag cactgcaggc cgttgtccgt atgctcaacc
    23101 cgttcacccc gcacatctgc ttcacgctgt ggcaggaact caaaggcgaa ggcgatatcg
    23161 acaacgcgcc gtggccggtt gctgacgaaa aagcgatggt ggaagactcc acgctggtcg
    23221 tggtgcaggt taacggtaaa gtccgtgcca aaatcaccgt cccggtggac gcaacggaag
    23281 aacaggttcg cgaacgtgct ggccaggaac atctggtagc aaaatatctt gatggcgtta
    23341 ctgtacgtaa agtgatttac gtaccaggta aactcctcaa tctggtcgtt ggctaagcgc
    23401 gggaggaagc gtgcgatatc tggcaacact gttgttatct ctggcggtgt taatcaccgc
    23461 cgggtgtggc tggcatctgc gtgacaccac gcaggttcct tccactatga aggtcatgat
    23521 cctggactca ggcgatccga acgggccatt aagccgtgcg gtgcgtaacc agttacgtct
    23581 gaatggtgtc gagttgcttg ataaagaaac cacgcgtaag gacgttccat ccctgcgttt
    23641 gggtaaagtg agcatcgcga aagataccgc atcggtattc cgtaacggtc aaacagcaga
    23701 gtatcagatg atcatgacgg tgaatgcgac cgtgttgatc cccggccgtg atatctaccc
    23761 gattagcgcc aaagtcttcc gttcattctt cgataacccg caaatggcgt tagcgaaaga
    23821 taacgaacaa gacatgatcg taaaagagat gtacgaccgt gctgccgaac agctgattcg
    23881 taagctgcca agcatccgtg ctgcggatat tcgttccgac gaagaacaga cgtcgaccac
    23941 aacggatact ccggcaacgc ctgcacgcgt ctccaccatg ctgggtaact gatgattcgg
    24001 ttgtacccgg aacaactccg cgcgcagctc aatgaagggc tgcgcgcggc gtatctttta
    24061 cttggtaacg atcctctgtt attgcaggaa agccaggacg ctgttcgcca ggtagctgcg
    24121 gcacaaggat tcgaagaaca ccacactttt tccattgatc ccaacactga ctggaatgcg
    24181 atcttttcgt tatgccaggc tatgagtctg tttgccagtc gacaaacgct attgctgttg
    24241 ttaccagaaa acggaccgaa tgcggcgatc aatgaacaac ttctcacact caccggactt
    24301 ctgcatgacg acctgctatt gatcgtccgc ggtaataaat taagcaaagc gcaagaaaat
    24361 gccgcctggt ttactgctct tgcgaatcgc agcgtgcagg tgacctgtca gacaccggag
    24421 caggctcagc ttccccgctg ggttgctgtg cgcgcaaaac agctcaactt agaactggat
    24481 gacgcggcga atcaggtgct ctgctactgt tatgaaggta acctgctggc gctggctcag
    24541 gcactggagc gtttatcgct gctctggcca gacggcaaac tgacattacc gcgcgttgaa
    24601 caggcggtga atgatgccgc gcatttcacc ccttttcatt gggttgatgc tttgttgatg
    24661 ggaaaaagta agcgcgcgtt gcatattctt cagcaactgc gtctggaagg cagcgagccg
    24721 gttattttgt tgcgcacatt acaacgtgaa ctgttgttac tggttaacct gaaacgccag
    24781 tctgcccata cgccactgcg tgcgttgttt gataagcatc gggtatggca gaaccgccgg
    24841 ggcatgatgg gcgaggcgtt aaatcgctta agtcagccgc agttacgtca ggccgtgcaa
    24901 ctcctgacac gaacggaact caccctcaaa caagattacg gtcagtcagt gtgggcagag
    24961 ctggaagggt tatctcttct gttgtgccat aaacccctgg cggacgtatt tatcgacggt
    25021 tgatatgaaa tctttacagg ctctgtttgg cggcaccttt gatccggtgc actatggtca
    25081 tctaaaaccc gtggaaacgc tggcgaattt gattggtctg acgcgggtca caatcatccc
    25141 taataatgtt cctccgcatc gtccccagcc ggaagcgaac agcgtgcagc gtaaacacat
    25201 gcttgaactg gcgattgccg acaagccatt atttactctt gatgaacgcg agctaaagcg
    25261 caatgccccc tcttacactg cgcaaacact gaaagagtgg cggcaggaac aaggaccgga
    25321 cgtgccgctg gcgtttatta ttggtcagga ttcattgctg acctttccga cctggtacga
    25381 atacgaaacg atactcgaca atgcacattt gatcgtctgt cggcgtccag gttacccact
    25441 tgaaatggcg caaccgcaat accagcaatg gctggaagat catttgacac ataacccgga
    25501 agatcttcac cttcagcctg ccggtaaaat ttatctggct gaaacgccgt ggtttaacat
    25561 ctcggcgacc atcatccgcg aacgtttgca aaacggtgaa tcgtgtgagg atttattgcc
    25621 ggaaccggta ttgacttaca ttaaccaaca aggcttgtat cgctgatacc tgtcgttacg
    25681 gagggcatga tgcgactgtg gttaattcgt catggcgaaa cacaagcgaa tgtcgatggt
    25741 ctgtacagcg gtcatgcgcc cacacccctg accgcacgcg gtattgagca agcgcaaaat
    25801 ctgcatacac tgctagatga tgtttccttt gacttggttt tatgcagtga actggaacgg
    25861 gcacagcata ccgcgcgact ggttctcagt gaccgccagc tccccgttca tatcatccct
    25921 gaactcaacg aaatgttttt tggcgactgg gagatgcgac atcatcgcga cctcatgcaa
    25981 gaagatgccg aaaactatag cgcgtggtgc aatgactggc agcatgctat ccccacgaac
    26041 ggtgaaggat ttcaggcatt ttcgcaacgt gtggaacgct ttatcgcaag gcttagtgaa
    26101 tatcagcact atcagaatat tttaatcgtc agccatcagg gcgtactgag tctgttaatt
    26161 gctcgtttaa ttggcatgcc tgccgagtct atgtggcatt ttcgcgttga ccaggggtgt
    26221 tggagcgcca ttgatatcaa ccaaaaattc gcgacgctac gcgtcctcaa tagccgtgcc
    26281 atcggggtcg agaacgcatg acttttctgt ttttttacgg gtaagccgca acgaccattg
    26341 acagccccgg gcaggctgat attctccgca gccagacttt ttccgccaga cacgactttg
    26401 tagaaattgt tttacaaaaa tggcgatgca atctgcggcg cggggtggga tgatagccca
    26461 ctttcgaaag ccgattcggc gacaattgtc ccgaaatcgc ctctggttca ggtatactga
    26521 cagaccattt ttatctattt gattcaccca gggggaaaac ttgcagggta aagcactcca
    26581 ggattttgtt atcgacaaaa ttgatgacct caaaggtcag gacatcatcg ccttagacgt
    26641 tcaaggcaaa tccagcatca ccgactgcat gatcatctgt acgggtacgt ccagccgtca
    26701 tgttatgtcc attgctgacc acgttgtgca ggagtctcgc gcagcgggcc tgttaccgct
    26761 cggcgtagaa ggtgaaaaca gcgccgactg gattgtcgtg gatttgggcg atgtgattgt
    26821 ccatgtcatg caggaagaga gccgtcgcct gtatgaactg gaaaaactct ggagttaatg
    26881 cgtgaagctg caacttgtcg ccgtgggaac gaaaatgccg gactgggtac aaaccggttt
    26941 taccgagtac ctgcgtcgtt ttccgaaaga tatgcccttc gagctgattg aaattccggc
    27001 cggcaaacgc ggcaagaatg cggacatcaa gcgcatactc gacaaagagg gtgagcagat
    27061 gttggcggcc gcaggcaaaa accgcattgt caccctcgat attccaggca agccctggga
    27121 tacgccgcag ttagccgctg agctggaacg ctggaagctg gatggtcgcg acgtaagtct
    27181 actgattggc gggcctgaag ggttgtcgcc tgcctgtaaa gcggcggctg agcagagctg
    27241 gtcgctgtcg gcgcttaccc tcccccatcc gctggttcgc gtgctggtcg cagagagtct
    27301 gtaccgggcg tggagcatca ccaccaacca tccttatcac cgtgagtgat aagggagctt
    27361 tgagtagaaa acgcagcgga tgaaactaca gaactctttt cgcgactata cggcagagtc
    27421 cgcgctgttt gtgcgccggg cgctggtcgc ctttttgggg attttgctgc tgaccggcgt
    27481 gcttatcgcc aacctgtata atctgcaaat tgttcgcttt accgactacc agacccgctc
    27541 taatgaaaac cgcattaagc tggtgcctat cgcgcccagc cgcggcatta tctacgaccg
    27601 taacggtatc cctctggccc tcaaccgcac tatctaccag atagaaatga tgccggagaa
    27661 agtcgataac gtgcagcaaa cgctggacgc tttgcgcagc gtggtagatc tgaccgatga
    27721 cgatattgct gcattccgaa aagagcgcgc acgttcacac cgtttcacct ctattccggt
    27781 gaaaactaac ctaaccgaag tacaagtagc tcgctttgcc gtcaatcagt accgttttcc
    27841 gggtgtcgaa gttaaaggct ataaacgtcg ttactatcct tacggttcgg cgttgaccca
    27901 cgtcatcggc tatgtgtcga aaatcaacga taaagacgtc gaacgcctga ataatgacgg
    27961 caaactggcc aactatgcgg caacgcatga tattggtaag ctgggcattg agcgttacta
    28021 tgaagacgtg ctgcacggtc agaccggtta tgaagaggtt gaagttaaca accgtgggcg
    28081 tgttatccgc cagttaaaag aagtaccacc gcaagccgga cacgatattt acctgacgct
    28141 ggatctcaaa ctccagcaat atattgaaac gctgctggcg ggtagccgcg cagctgtggt
    28201 tgttaccgaa ccgcgtacag gtggggtgct ggcgctggtt tccacgccta gttatgaccc
    28261 aaacctgttt gttgacggta tctccagcaa agattattcc gccttgctga acgacccgaa
    28321 tacaccgctg gtgaaccgcg ccacacaggg ggtttatcct cccgcgtcta cagttaaacc
    28381 ctatgtggcg gtttcggcat taagcgccgg ggtgatcacg cgcaatacga cgctgtttga
    28441 cccaggctgg tggcaactac cagggtcgga aaaacgttat cgtgactgga aaaaatgggg
    28501 ccacgggcgt ctgaatgtca caagatcgct ggaagaatct gcggatacct tcttctatca
    28561 ggtggcctac gatatgggga tcgatcgcct ctccgaatgg atgggtaaat tcggttatgg
    28621 tcattacacc ggtatcgacc tggcggaaga acgttccggc aacatgccta cccgcgaatg
    28681 gaaacagaaa cgctttaaaa aaccgtggta tcagggtgac accattccgg ttggtatcgg
    28741 ccagggttac tggacagcga ccccaatcca gatgagtaag gcactgatga tcctgattaa
    28801 tgacggtatc gtgaaggttc cgcatttgct gatgagcacc gccgaagacg gcaaacaggt
    28861 gccatgggta caaccgcatg aaccgcccgt cggcgatatt cattccggtt actgggagct
    28921 ggcgaaagac ggtatgtacg gtgttgctaa ccgccctaat ggtacggcga ataaatactt
    28981 tgccagcgca ccgtacaaaa ttgcggcgaa atctggtacc gctcaggtct tcggtctgaa
    29041 agcgaacgaa acctataatg cgcacaaaat tgccgagcgt ttacgtgacc acaaactgat
    29101 gaccgccttt gcgccataca acaatccgca agtggctgtc gccatgattc tggagaacgg
    29161 cggcgcgggt ccggcggttg gtacgctgat gcgccagatc ctcgaccaca ttatgctggg
    29221 tgataacaac accgatctgc ctgcggaaaa tccagcggtt gccgcagcgg aggaccatta
    29281 atcatgacgg ataatccgaa taaaaaaaca ttctgggata aagtccatct cgatcccaca
    29341 atgctgctga ttttactggc attgctggtt tacagcgccc tggttatctg gagcgccagc
    29401 ggtcaggata ttggcatgat ggagcgtaaa atcggccaaa tcgcgatggg tctggtcatc
    29461 atggtggtga tggcgcaaat tcctccacgc gtttatgaag gctgggcccc ctatctctat
    29521 atcatctgta ttattttgct ggtggcggta gatgctttcg gagccatctc taaaggcgct
    29581 caacgctggc tggacctcgg tattgttcgt tttcagccgt cggaaattgc caaaatagcc
    29641 gtaccactga tggttgcgcg ctttatcaac cgcgacgttt gcccgccatc gttgaagaat
    29701 actggcatcg cgctggtgct gatatttatg cccacgctgc tggtggctgc acagcctgac
    29761 ctgggaacat caatcctcgt tgcgctttcc ggtctgtttg tactgttcct ctctggcctt
    29821 agctggcgtc tgattggcgt cgcagtagtg ctggtagcgg cgttcattcc gattctgtgg
    29881 ttcttcctga tgcatgatta ccagcgccag cgcgtaatga tgctcctgga cccggaatca
    29941 gacccactcg gcgcgggcta tcacattatt cagtctaaaa ttgctattgg ctccggcgga
    30001 ttacgcggca aaggctggct gcacggcact cagtcacagc ttgaatttct ccccgaacgc
    30061 catactgact ttatcttcgc ggtactggcg gaagagctgg gattagtggg cattctgatt
    30121 ctgctcgctc tctacattct gctgatcatg cgcgggctgt ggatagccgc cagagcgcaa
    30181 accacctttg gtcgcgtcat ggctggtggc ttaatgctga tattattcgt ttatgtcttc
    30241 gtaaatattg gtatggtaag cggtattctg ccggttgtag gggttccgct cccactggtc
    30301 agttatggag gatcggcgct aattgtgctg atggctgggt tcgggattgt aatgtcaatc
    30361 cacacccaca ggaaaatgtt gtcgaaaagc gtgtaagagg tgcgcaatgc gtaagcagtg
    30421 gctcgggatc tgcatcgcgg caggaatgct cgcggcatgt acaagcgatg atggtcagca
    30481 acagacggta agtgtaccgc agcctgcggt atgtaacggc cccatagttg aaattagcgg
    30541 ggcggacccg cgtttcgaac cactgaacgc gacggcaaat caggattacc agcgcgacgg
    30601 taaaagctac aaaatcgtgc aggatccgtc tcgatttagc caggcgggac tggcggcaat
    30661 ctatgatgcc gaaccaggca gtaacctgac ggcctctggc gaagctttcg atccgacaaa
    30721 gctgacggcg gcccatccaa cgcttccgat ccccagctac gccagaatca ctaacctggc
    30781 taacgggcga atgatcgtgg tgcgcattaa tgatcgcggc ccttacggca acgaccgcgt
    30841 tatttcgctt tctcgcgcgg cagctgaccg tcttaacacg tcaaacaaca ccaaagttcg
    30901 tatcgatccg attattgtcg cccaggatgg ttcgctttct ggtcctggta tggcttgtac
    30961 cacagtcgcc aaacagactt acgccctgcc tgcacctccc gatttaagcg gtggcgcggg
    31021 aacaagttca gtgtctggcc cgcagggtga cattcttccg gtcagtaatt cgacgctaaa
    31081 aagcgaagat ccgaccggcg cgccggtaac cagtagcggt ttcctcggcg ctccaacgac
    31141 cttagcgcct ggtgtactgg aaggcagcga accgacgcct gcgccacagc ccgttgttac
    31201 agcttcgtcg acaacgcctg caacctcgcc tgcaatggtg acaccgcaag ccgcctcgca
    31261 aagcgccagc ggcaacttta tggtgcaagt cggggccgta agcgatcagg ctcgtgcgca
    31321 acagtaccaa cagcaactgg gacagaagtt cggcgtcccc ggtcgcgtaa ctcaaaatgg
    31381 cgcggtctgg cggatccagc ttggcccatt cgccagcaaa gccgaagcca gtaccttgca
    31441 gcaacgtttg caaaccgaag cccaattaca gtcatttatt actaccgcgc agtagcgtaa
    31501 agcaggcatc tgatgtgtca atttctgtaa atgacgttaa caaagtcacg cggaaagtca
    31561 gatgcctgcc ggtagtgcat ttgctatagt agggcacttt tttaattcca tcacggatgt
    31621 cgtagttcag accatgaata ccattttttc cgctcgtatc atgaagcgcc tggcgctcac
    31681 cacggctctt tgcacagcct ttatctctgc tgcacatgcc gatgacctga atatcaaaac
    31741 tatgatcccg ggtgtaccgc agatcgatgc ggagtcctac atcctgattg actataactc
    31801 cggcaaagtg ctcgccgaac agaacgcaga tgtccgccgc gatcctgcca gcctgaccaa
    31861 aatgatgacc agttacgtta tcggccaggc aatgaaagcc ggtaaattta aagaaactga
    31921 tttagtcact atcggcaacg acgcatgggc caccggtaac ccggtgttta aaggttcttc
    31981 gctgatgttc ctcaaaccgg gcatgcaggt tccggtttct cagctgatcc gcggtattaa
    32041 cctgcaatcg ggtaacgatg cttgtgtcgc catggccgat tttgccgctg gtagccagga
    32101 cgcttttgtt ggcttgatga acagctacgt taacgcactg ggcctgaaaa atacccactt
    32161 ccagacggta catggtctgg atgctgatgg tcagtacagc tccgcgcgag atatggcgct
    32221 gatcggccag gcattgatcc gtgacgtacc gaatgaatac tcgatctata aagaaaaaga
    32281 atttacgttt aacggtattc gccagctgaa ccgtaacggc ctgttatggg ataacagcct
    32341 gaatgtcgac ggcatcaaaa ccggacacac tgacaaagca ggttacaatc ttgttgcttc
    32401 tgcgactgaa ggccagatgc gcttgatttc tgcggtgatg ggtggacgta cttttaaagg
    32461 ccgtgaagcc gaaagtaaaa aactgctaac ctggggcttc cgtttcttcg aaaccgttaa
    32521 cccactgaaa gtaggtaaag agttcgcctc tgaaccggtt tggtttagtg attctgatcg
    32581 cgcttcgtta ggggttgata aagacgtgta cctgaccatt ccgcgtggtc gcatgaaaga
    32641 tctgaaagcc agctatgtgc tgaacagcag tgaattgcat gcgccgctgc aaaagaatca
    32701 ggtcgtcgga actatcaact tccagcttga tggcaaaacg atcgagcaac gcccgctggt
    32761 tgtgttgcaa gaaatcccgg aaggtaactt cttcggcaaa atcattgatt acattaaatt
    32821 aatgttccat cactggtttg gttaaaaatt gaacacttga aagtgtaatt tccgtcccca
    32881 tatactaagc atcagtaaaa aaactcccgc cttctggcgg gagttgctat ttaattacgt
    32941 tacgccggag ctgacatgaa aaccaaactt aacgaactgc ttgaattccc tactcctttt
    33001 acttacaaag ttatggggca ggcgttacct gagctggttg atcaggtggt tgaagtggta
    33061 cagcgccatg cgccaggtga ctacacccca acggtaaaac caagcagcaa aggcaactac
    33121 cactcggtat ctatcactat caacgccact catatcgagc aggttgaaac actgtatgaa
    33181 gaactgggca aaatcgatat tgtccgcatg gttctgtaac tcgcttctcc gttacccggc
    33241 tcctggtcgg gtaactcccc tctcctcgct gtgatatact tttcccccac ttttactcat
    33301 tctccacgga gatgccgttt tgtatcagga taaaattctt gtccgccagc tcggtcttca
    33361 gccttacgag ccaatctccc gggctatgca tgaattcacc gatacccgcg ataatagtac
    33421 ccttgatgaa atctggctgg tcgagcacta tccggtattc acccaaggtc aggcaggaaa
    33481 agcggagcac attttaatgc cgggtgatat tccggtgatc cagagcgatc gcggtgggca
    33541 ggtgacttat cacgggccgg gacaacaggt gatgtatgtg ttgcttaacc tgaaacgccg
    33601 taaactcggt gtgcgtgaac tggtgacctt gcttgagcaa acagtggtga ataccctggc
    33661 tgaactgggt atagaagcgc atcctcgggc tgacgcgcct ggtgtctatg tcggggaaaa
    33721 gaaaatttgc tcactgggtt tacgaattcg acgcggttgt tcattccacg gtctggcatt
    33781 aaacgtcaat atggatcttt caccattttt acgtattaat ccttgtgggt atgccggaat
    33841 ggaaatggct aaaatatcac aatggaaacc cgaagcgacg actaataata ttgctccacg
    33901 tttactggaa aatattttag cgctactaaa caatccggac ttcgaatata ttaccgctta
    33961 attccataca tcaatggccc aatttacact gggtcattac tcataatacc tgctaatgct
    34021 ttacatctcc ttcgcgtttg tatattttct ttatcacttg cagaaatgcg gtgccacgcc
    34081 ttgctccgta actcacccca ttgattaacc tattgaaata gttgattatg gtgagcaaaa
    34141 gcgttccgtt ccaattagac ataagattat tgtaattttt taaatattat ttttccatga
    34201 ataaaattgg agtaagtgcg tggatagtaa taatcaaatt gaaccctgtt taagcagaaa
    34261 gtcgtcagaa ggtaaaccac aaatatttac aaccttacga aatattgatc ttaaccttct
    34321 gactattttt gaagctgtat atgtacataa agggatcgtt aatgcagcga aagtgcttaa
    34381 tctgaccccc tcggcaatca gtcagtctat tcagaaactg cgcgttatat tccctgaccc
    34441 attgtttatt cgcaaaggcc agggtgtcac tcctaccgca tttgcgatgc atctacatga
    34501 gtatatcagt cagggccttg agtccattct tggcgcgctg gatatcgaag gaagctatga
    34561 taagcaacga acgataacta ttgcgaccac tccctcggtc ggagccctgg tccttcctgt
    34621 catctatcgg gcgattaaaa ctcactatcc gcagctttta ctgcgcaacc cgcccatcag
    34681 cgacgcggaa aaccaactca gtcagtttca aaccgatctc atcatcgata acatgttttg
    34741 caccaatcgt acggtgcaac atcatgttct gttcaccgac aatatggtgt taatttgccg
    34801 caagggaaat ccactactct ctttagaaga tgacagagag actatcgaca acgctgcgca
    34861 tgtacttctg ttaccggaag ggcaaaattt cagcggtctg cggcagagag ttcaagagat
    34921 gtttccggac cggcaaatca atttcaccag ctacaacatt ttgacaatcg ctgcactggt
    34981 tgccaacagt gacatgttag cgattattcc cagccgtttt tataacctgt ttagtcgttg
    35041 ctggccgctg gaaaaactgc cttttccgtc cttaaatgag gagcaaatag atttctctat
    35101 ccactacaac aaattcatcc tgcgtgatcc catcctgcac ggagtaatag atgttatccg
    35161 taatgcattt tgaaaaagta ttgttacagc cagatcacag cgcagaacga taaaatggca
    35221 caatccaaca acaattttac attttcgcga ccgctttggc tgctttcagg tccgtttcaa
    35281 tgatatactg ccagtcgtta attcaaaaat agttgataat tacaacaatc tattgaattg
    35341 aaacgctttc cttcgtaatt cgcaactgga acacgcacgc tatgagtaaa cccattgtga
    35401 tggaacgcgg tgttaaatac cgcgatgccg ataagatggc ccttatcccg gttaaaaacg
    35461 tggcaacaga gcgcgaagcc ctgctgcgca agccggaatg gatgaaaatc aagcttccag
    35521 cggactctac acgtatccag ggcatcaaag ccgcaatgcg caaaaatggc ctgcattctg
    35581 tctgcgagga agcctcctgc cctaacctgg cggaatgctt caaccacggc acagcaacgt
    35641 ttatgatcct cggcgctatt tgtacccgcc gttgtccgtt ctgtgatgtt gcccacggtc
    35701 gcccggtagc tcctgatgcc aatgaaccag tgaaactggc gcagaccatt gccgatatgg
    35761 cgctgcgtta tgtggttatc acctccgttg accgtgatga cctgcgcgat ggcggtgccc
    35821 aacactttgc ggattgtatt actgccattc gggaaaaaag cccgcaaatc aaaattgaaa
    35881 ctctggtgcc ggatttccgc ggtcgaatgg atcgtgctct ggatattctg actgcaacgc
    35941 ccccagatgt gttcaaccat aacctggaaa acgtaccgcg tatttaccgg caggtacggc
    36001 ctggcgcaga ttacaactgg tcgctgaagc tgctggaacg ctttaaagaa gcgcatccgg
    36061 aaatcccgac caagtctggt ctgatggtgg gtctgggtga aaccaatgaa gaaattattg
    36121 aggtaatgcg cgacctgcgc cgtcatggtg tgacgatgtt aacgctgggg caatatttgc
    36181 agccaagccg ccatcacctg ccggttcaac gttacgttag cccggatgag ttcgacgaaa
    36241 tgaaagccga agcgctggcg atgggcttta cccatgctgc atgcggtccg tttgtccgct
    36301 cttcttacca cgccgatttg caggcgaaag gaatggaagt taagtaatcc tgtaataatt
    36361 acttacatct gtctgataaa aaacccgctt tttgaagcgg gttttttgta tcgaacaaga
    36421 tattgaggga gcgtcctgct cgccacgtca ctctttatga gagagctttt cagcctgaag
    36481 atcaacgtct gcgccttttt tcgccgcagc atcgtcatca ttcatcgcct tcttgaaccc
    36541 tttaatggcc gctccaaggt ctccgcccag cgtacgtaac ttcttagtcc caaacagcag
    36601 aacgaccagc gccgcaacta ccagcagttt ggtaatacta atctcaccca tagatacctt
    36661 cttgacatat aaccggcaac agaacgcgcc ttaaaaacca gagaatatta acggtcattc
    36721 gacgcgcgca cacaatagca atctgtaaca aggtgaatca agcgccgagt aaaaaaacat
    36781 cataataatt gcggcggcgc aaagcgacgg ttgtttaaga tgggcagttg cgcgcgcacc
    36841 tgacgcacac gttcgggcgt cacttccgcc ataatgagtg caggcatttc tgacgctgcc
    36901 gcaatggtga cgccaaaggg atctataatc cggctttgcc cgatattttt gttcccgcac
    36961 tcccccgccg ccaccatata acaggtggta tccagcgcac gagcggcaag caacgttgac
    37021 cagtgatgct ctttgagcgg cccacgaacc caggcggcag gaagtaccag gatttcagct
    37081 ccctgtaaag cctgtgccag cgccagctct ggaaagcgta agtcataaca ggtcatcaga
    37141 ccgaccttca tcccttccac ctccagtaac ggagctattt cattaccagc atcaacatgg
    37201 cgtgattcct gaatggcaaa tgcatcatag agatgcagtt tggcataacg ggcgacgatg
    37261 tttcctgcct gaagtgccac cagcatattc catgcgcgcc ccggcgttga aggaagatga
    37321 atcgtcagaa ttgtcgtcat catgttacgt ttactttctc gccgtaaacg tccgaggaat
    37381 tcgccttcca gcagctgtgc tgatttaacc gatagatctg catcatgatc atcgcgcgcc
    37441 agcaatgctt ccggcaggac aaacagcgat acgtcgtttt ccgccgcctg cgccatcaac
    37501 gaggcacaaa tctcagcgtt cttttcccac acagatgtaa cagcaaactg tcctgcagca
    37561 accagcattg ccacctcctg tcagagtcgt aaaattcgcc aacgacgatt attcggcgtt
    37621 acacttatca ctcatacaaa tcaaatagca ggattttgca gtgttacaac ttcttttagc
    37681 agtttttatt ggcggtggta cgggaagcgt agcgagatgg ctgttaagta tgcgatttaa
    37741 cccgctgcat caggcgattc cgttggggac gctggcagca aacctgattg gggcattcat
    37801 cataggaatg ggattcgcct ggttcagcag gatgacgaac attgatccag tgtggaaagt
    37861 attaatcacc accggatttt gtggcggtct aacaaccttc tcaacatttt cggcagaagt
    37921 ggtgtttttg ttacaagagg gccgctttgg ctgggcatta ctgaacgttt tcgtcaacct
    37981 tctggggtct tttgccatga ccgcactggc attctggctg ttttcggcct caaccgtaca
    38041 ctaaagacca aaaaaaaccc gctgattaag cgggttttga attcttgctg acgtatctta
    38101 cagagcgatt acgtttgcag cagaagggcc tttggcaccg ttagtgattt cgaactctac
    38161 gcgctgacct tcagcaagag ttttaaaacc attagtctgg attgcagaga agtgtacgaa
    38221 cacgtctttg ctgccgtctt ccggagtaat gaaaccgaat cctttggact cattaaacca
    38281 cttaacgtta cctttaatct tagacatcaa aattaccttt acatgaaaaa tagacacaaa
    38341 tgctgtgtcg gttatcagta cac
//
LOCUS       DAZPAB010000017        37525 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZPAB010000017 DAZPAB010000000
VERSION     DAZPAB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.017"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(24..278)
                     /locus_tag="ACHUPG_000796"
     CDS             complement(24..278)
                     /locus_tag="ACHUPG_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR0860519.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="ACHUPG_000797"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="ACHUPG_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR0860520.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="ACHUPG_000798"
     CDS             1228..2694
                     /locus_tag="ACHUPG_000798"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR0860521.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="ACHUPG_000799"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="ACHUPG_000799"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR0860522.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="ACHUPG_000800"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="ACHUPG_000800"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR0860523.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="ACHUPG_000801"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="ACHUPG_000801"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR0860524.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="ACHUPG_000802"
     CDS             5709..7265
                     /locus_tag="ACHUPG_000802"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR0860525.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="ACHUPG_000803"
     CDS             7347..9161
                     /locus_tag="ACHUPG_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR0860526.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="ACHUPG_000804"
     CDS             9162..10256
                     /locus_tag="ACHUPG_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR0860527.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="ACHUPG_000805"
     CDS             10256..11281
                     /locus_tag="ACHUPG_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0860528.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="ACHUPG_000806"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="ACHUPG_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR0860529.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="ACHUPG_000807"
     CDS             complement(12876..13220)
                     /locus_tag="ACHUPG_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR0860530.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14749)
                     /gene="bcr"
                     /locus_tag="ACHUPG_000808"
     CDS             complement(13553..14749)
                     /gene="bcr"
                     /locus_tag="ACHUPG_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFR0860531.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVL
                     VWLSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVL
                     SYMLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVR
                     RIGALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVIL
                     DEFPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYA
                     SRPKKR"
     gene            complement(14777..15472)
                     /gene="rsuA"
                     /locus_tag="ACHUPG_000809"
     CDS             complement(14777..15472)
                     /gene="rsuA"
                     /locus_tag="ACHUPG_000809"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR0860532.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15622..17382
                     /locus_tag="ACHUPG_000810"
     CDS             15622..17382
                     /locus_tag="ACHUPG_000810"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR0860533.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17507..17791
                     /gene="rplY"
                     /locus_tag="ACHUPG_000811"
     CDS             17507..17791
                     /gene="rplY"
                     /locus_tag="ACHUPG_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR0860534.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17930..18937)
                     /gene="yejK"
                     /locus_tag="ACHUPG_000812"
     CDS             complement(17930..18937)
                     /gene="yejK"
                     /locus_tag="ACHUPG_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR0860535.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19119..19346
                     /locus_tag="ACHUPG_000813"
     CDS             19119..19346
                     /locus_tag="ACHUPG_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR0860536.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19366..21126
                     /gene="yejM"
                     /locus_tag="ACHUPG_000814"
     CDS             19366..21126
                     /gene="yejM"
                     /locus_tag="ACHUPG_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR0860537.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21201..21277
                     /locus_tag="ACHUPG_000815"
     tRNA            21201..21277
                     /locus_tag="ACHUPG_000815"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21235..21237,aa:Pro,seq:ggg)
     gene            complement(21380..23971)
                     /gene="yejO"
                     /locus_tag="ACHUPG_000816"
     CDS             complement(21380..23971)
                     /gene="yejO"
                     /locus_tag="ACHUPG_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFR0860538.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24291..24938
                     /gene="narP"
                     /locus_tag="ACHUPG_000817"
     CDS             24291..24938
                     /gene="narP"
                     /locus_tag="ACHUPG_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR0860539.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25149..26201)
                     /gene="ccmH"
                     /locus_tag="ACHUPG_000818"
     CDS             complement(25149..26201)
                     /gene="ccmH"
                     /locus_tag="ACHUPG_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR0860540.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26198..26755)
                     /gene="dsbE"
                     /locus_tag="ACHUPG_000819"
     CDS             complement(26198..26755)
                     /gene="dsbE"
                     /locus_tag="ACHUPG_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR0860541.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26752..28695)
                     /gene="ccmF"
                     /locus_tag="ACHUPG_000820"
     CDS             complement(26752..28695)
                     /gene="ccmF"
                     /locus_tag="ACHUPG_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR0860542.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28692..29171)
                     /gene="ccmE"
                     /locus_tag="ACHUPG_000821"
     CDS             complement(28692..29171)
                     /gene="ccmE"
                     /locus_tag="ACHUPG_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR0860543.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29168..29377)
                     /gene="ccmD"
                     /locus_tag="ACHUPG_000822"
     CDS             complement(29168..29377)
                     /gene="ccmD"
                     /locus_tag="ACHUPG_000822"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR0860544.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29374..30111)
                     /gene="ccmC"
                     /locus_tag="ACHUPG_000823"
     CDS             complement(29374..30111)
                     /gene="ccmC"
                     /locus_tag="ACHUPG_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR0860545.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30153..30815)
                     /gene="ccmB"
                     /locus_tag="ACHUPG_000824"
     CDS             complement(30153..30815)
                     /gene="ccmB"
                     /locus_tag="ACHUPG_000824"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR0860546.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30812..31435)
                     /gene="ccmA"
                     /locus_tag="ACHUPG_000825"
     CDS             complement(30812..31435)
                     /gene="ccmA"
                     /locus_tag="ACHUPG_000825"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR0860547.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHR
                     DGDAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31448..32050)
                     /gene="napC"
                     /locus_tag="ACHUPG_000826"
     CDS             complement(31448..32050)
                     /gene="napC"
                     /locus_tag="ACHUPG_000826"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR0860548.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32060..32509)
                     /gene="napB"
                     /locus_tag="ACHUPG_000827"
     CDS             complement(32060..32509)
                     /gene="napB"
                     /locus_tag="ACHUPG_000827"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR0860549.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32506..33369)
                     /gene="napH"
                     /locus_tag="ACHUPG_000828"
     CDS             complement(32506..33369)
                     /gene="napH"
                     /locus_tag="ACHUPG_000828"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR0860550.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33356..34051)
                     /gene="napG"
                     /locus_tag="ACHUPG_000829"
     CDS             complement(33356..34051)
                     /gene="napG"
                     /locus_tag="ACHUPG_000829"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR0860551.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34058..36544)
                     /gene="napA"
                     /locus_tag="ACHUPG_000830"
     CDS             complement(34058..36544)
                     /gene="napA"
                     /locus_tag="ACHUPG_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR0860552.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36541..36804)
                     /gene="napD"
                     /locus_tag="ACHUPG_000831"
     CDS             complement(36541..36804)
                     /gene="napD"
                     /locus_tag="ACHUPG_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR0860553.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36794..37288)
                     /gene="napF"
                     /locus_tag="ACHUPG_000832"
     CDS             complement(36794..37288)
                     /gene="napF"
                     /locus_tag="ACHUPG_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR0860554.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37397..>37525
                     /locus_tag="ACHUPG_000833"
     CDS             37397..>37525
                     /locus_tag="ACHUPG_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0860555.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPE"
BASE COUNT         9157 a        10149 c         9273 g         8946 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgacctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttatg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccatgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc gacagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgact atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgagg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccgcagcc agcccgtgga agaaacgcat cacaatcagc tgatcgatgg
    14461 tttgtgccaa cgcacacgcc accgcggcgg cggcaaacac cagcgtcccg ccaagaacca
    14521 ccggcttacg cccgaagctg tctgccatcg gcccgtagat taactgcccc aacgcaaagc
    14581 ccagaatata agtactgagg gtcatctgcg tactgcccgc cgatacgcca aactgcgctg
    14641 aaattaccgg tagcgcgggc agatacatat caatcgacag cggcatcaac atggccagca
    14701 ggccaaggat aaaaacaata gcaaacgacg aatgctgtcg ggtggtcaca acgggctcct
    14761 gaaagtcatt gaaaagttag acgacgctgg caatttcttc ttcagttaac ggacgatatt
    14821 caccgggggc taaatcagca tccagcgtaa taccgccaat acgttcacga tgcagctcaa
    14881 ccacgtggtt acccacggcg gcgaacatgc gtttcacctg atgataacgc ccttcgctga
    14941 tggtcagacg aacctgcgtt ggggtaatca cttcaagcac cgccggctta gtgagatctt
    15001 tttcgttatg cagctgcacg cctttagcaa attgctctgc cgtatcgtca gctacaggtg
    15061 attccagtgt caccagatag gtcttctcgc aatgatggcg cggagaagta atgcggtgcg
    15121 accactgacc atcatcagtc atcagcacca gaccggtggt atcgatatcc agacgtcctg
    15181 cggcgtgcag tttccacgct accggttcat ccagaaaata gagcaccgtc gggtgatccg
    15241 ggtcgtccgt ggagcaaaca tagccctgag gcttattgag catgaagtaa cgtggaccgt
    15301 gttgctgcgc cagcgggttg ccatcgtaag cgacatcatg ttcagggagc agtttgaacg
    15361 ctgcattacg aacgatttcg ccatcgacgg tgacacgatt gccgcggatt tcacgcccgg
    15421 caatagcacg gctaacgccg agttgctgtg cgataaattt atcaagtcgc atgtgtgtga
    15481 ttttgcctgt aaaaaacgga ggtcgggtaa tgacccgaaa atctgaactg ttgtctgccc
    15541 agtatagcgg tctaataact tccctcaagg gaaaaagaac cgtggcatac tattagcaga
    15601 ataatctacc tacgcgaaac catgattttt acactccgcc catatcagca agaagccgtg
    15661 gatgccacgc tcaaccattt tcgtcgtcat aaaacccctg ccgttatcgt gctgcccacc
    15721 ggcgcaggta aaagcctggt gatagcggaa ctggcacggc tggcgcgtgg tcgcgtactg
    15781 gtgctggcac acgttaaaga actggtggcg caaaaccatg caaagtatca ggcgctgggg
    15841 ctggaagccg atatttttgc cgccgggcta aagcgcaaag agagccacgg taaagtggta
    15901 tttggcagcg ttcagtcggt cgcccgtaat cttgatgcct ttcagggtga attttcgctg
    15961 ttgattgtcg atgaatgcca ccgtattggt gacgatgaag agagccagta tcagcaaatc
    16021 ctcactcacc tgactaaagt gaatccccac ttacgcctgc tggggctgac tgccacgcct
    16081 tttcgattgg gcaaaggctg gatctaccag ttccattatc acggcatggt acgcggcgat
    16141 gagaaagccc ttttccgtga ctgcatttat gagctgccgc tgcgttatat gattaaacac
    16201 ggctatctga cgccgccaga acgactggat atgccagtag tgcaatacga tttcagccgc
    16261 ttgcaggcac agagtaacgg gctgttcagc gaagctgatc tcaaccgtga gctgaaaaaa
    16321 cagcaacgca ttacgccaca tatcatcagc cagatcatgg agttcgccga aaagcgcaaa
    16381 ggggtaatga ttttcgccgc caccgttgaa cacgcaaaag agattgtggg attactgcct
    16441 gccgaagatg ctgcactgat tactggcgac aaccccggcg ctgagcgcga tgtgttaatt
    16501 gaagatttta aagcccagcg ttttcgctat ctggtcaacg tcgcagtact gaccaccgga
    16561 tttgacgccc cgcacgtcga tcttatcgcc attctgcgcc ctaccgaatc agtgagtctt
    16621 taccaacaaa ttgtcgggcg cggtctgcgt ctcgctccgg gcaagactga ttgtttaatt
    16681 cttgattatg cgggtaatcc tcacgatctc tacgcgccgg aagttggtac accgaaaggc
    16741 aaaagtgaca acgttccggt acaggttttc tgccctgcct gcggttttgc caacaccttt
    16801 tgggggaaaa cgaccgccga cgggacattg attgaacact ttggtcgccg ctgtcaggga
    16861 tggtttgaag atgacgacgg tcatcgcgaa cagtgtgact tccgtttccg ttttaaaaat
    16921 tgcccgcaat gtaacgcaga aaatgatatt gccgctcgcc gctgccgcga atgtgacacg
    16981 gtactggttg atccggatga tatgttaaaa gcggcgctac gactgaaaga tgcgctggta
    17041 ttacgctgta gcggcatgtc tttgcagcat gggcacgacg agaaaggcga atggttgaaa
    17101 atcacctatt acgatgaaga cggcgcggat gtgagtgagc gtttccgtct gcaaacgccc
    17161 gcccagcgta ccgccttcga gcagcttttt atccgcccgc atacgcgcac accgggcatc
    17221 cctctgcgct ggatcaccgc cgccgatatc ctcgcccagc aagccttact gcgacatccg
    17281 gattttgtcg tcgcccgcat gaaaggtcag tactggcaag tgcgtgaaaa agtgttcgat
    17341 tacgaaggtc gttttcgtcg ggcgcacgaa ttacgcggtt aatggtagtt ttgattgatg
    17401 tataaggcga ttgagtatag aatctcgccc gcttttgcat acgcaaagca gatcacttac
    17461 ctgttgctgg gtcgcctgta gcaggattaa tttaagagag aaagaaatgt ttactatcaa
    17521 cgcagaagta cgtaaagagc agggtaaggg tgcgagccgc cgcctgcgtg ccgctaacaa
    17581 gttcccggca atcatctacg gtggcaaaga agcaccgctg gctatcgagc tggatcacga
    17641 caaagtcatg aacatgcaag ctaaagctga attctacagc gaagttctaa ccatcgttgt
    17701 tgacggtaaa gaaatcaaag ttaaagctca ggacgtacag cgtcacccgt acaaaccgaa
    17761 gctgcagcac atcgacttcg ttcgcgctta attgctgaat aagttgtaaa aaaccccgct
    17821 ccggcggggt tttttgtatc tgcagattat gcctgattac ggtattgcta ttttttgcag
    17881 gccagataag gcgttcacgc cgcatctggc attcagaaca caaaaccgat taattgccgc
    17941 cagacgtccg gcgctgcaat tggtcgcgca aattcggcgg cgtgccttta atggtcaagg
    18001 tatcggttgc tggatcccag aagatgcgtt cacctaacag catcgcatca aagttaatag
    18061 tcaagccacc gccgctaccg gcaaacttcg tcaactgacg cagtgtgcta cggtctgccg
    18121 ggaagctctc ttccagttcg tagccttttt cagccgcaaa ctccgtgaag ctcacttcgc
    18181 ttacgcctgc cagctctttc gacagcgatt ccagttcgat ctcttcgcct gcctgcagct
    18241 gctcattaca gtagctataa acctgctggc gcacgttctg tcgctctgct ttatccagtt
    18301 gtgcttcggc agtgaaatca tcaaccgctt gcaacagccc acggttttgc gctttcgcgt
    18361 tcaatccttc gctggccccg aggaaatcca taaagaaatc cgccactttg cgccctactc
    18421 gccctttcag gaaagtgaga tagcgggtgg actctggatt ggtttcccat tcggttaaat
    18481 cgatacgcgc aacaatatcc gcatgattga tatcaagata atgggttggg ttgatgtcga
    18541 gattttcgtt aacgcgcata ctgctcaggt tgctcagaac tgccaccagc aaatactcca
    18601 ccgccagata acggtagtgg caaaacagca cgaatccacc gtcagcaaaa ggatatttcg
    18661 ctaattcgtc acgcaggcga ccggttgccg cgcggctaaa tgccagaaag tcctcttcgc
    18721 cctgacgctg taagcgcagc gtctgcgcca gttcactctc ttcgctaaac aagccgtaag
    18781 ctttattttt ggcgctatag acccgatgca gttctgccac catctcaacg acggtttctg
    18841 tcggttccag caatgaatcg cgcaacacca gctcaaggtt ctgctcatca cgcttgataa
    18901 gctggtgcag agcaatctgg ttgatatcca gactcatgat aaactctcct ttaagaccgg
    18961 gcggtattca accaccgcca gttaagcgac gcaataaaag atggcggtca ttttacagaa
    19021 ggcagcgtaa cgagaatgat ttaacgcata aaaaagcaga aaaaaaaccg ttgctacggt
    19081 aatatgttgc cctttcatca actaactgat ttcgatttat gccacaaatt tcccgctaca
    19141 gtaatgaaca ggttgaacaa ttgctcgcag agctgctcaa cgtactggaa aaacataagg
    19201 ctccgaccga cctttccctg atggtgttag gcaacatggt cactaacctt atcaacacca
    19261 gcattgcccc ggcccaacgc caggcaattg ccaactcttt tgccagcgcc ttacagtcct
    19321 ctatcaacga agacaaagcg cactaaggga aacagataac aggttatggt aactcatcgt
    19381 cagcgctacc gtgaaaaagt ctcccagatg gtcagttggg ggcactggtt tgcactgttc
    19441 aatattctgc tttcgctcgt cattggcagc cgttacctgt ttatcgccga ctggccgaca
    19501 acgcttgctg gtcgcattta ttcctacgta agcattatcg gccatttcag cttcctagtg
    19561 ttcgccacct acttgctgat cctcttcccg ctgaccttta tcgtcggctc ccagaggctg
    19621 atgaggtttt tgtccgtcat tctggcgacg gcgggaatga cgctattact gatcgatagc
    19681 gaagtcttta ctcgtttcca tctccatctt aatcccatcg tctggcagtt ggttatcaac
    19741 ccagacgaaa atgagatggc gcgcgactgg cagctgatgt tcatcagcgt gccggttatt
    19801 ttattgcttg aactggtgtt tgcgacgtgg agctggcaaa agctgcgcag cctgacgcgt
    19861 cgtcgacgct tcgcgcgccc gctggccgca ttcttattta tcgcctttat cgcctcgcat
    19921 gtggtgtata tctgggccga tgccaacttc tatcgcccga tcaccatgca gcgcgctaac
    19981 ctgccgcttt cgtacccgat gacggcgcga cgtttccttg agaagcatgg cctgcttgat
    20041 gcgcaggagt atcaacgccg tcttattgag caaggtaatc cagacgccgt ttccgtacag
    20101 tatccgttaa gcgaactgcg ctatcgcgat atgggcaccg ggcagaatgt gctgttgatt
    20161 actgtcgatg gcctgaacta ctcacgcttc gagaagcaga tgcctgcgct ggcaggtttt
    20221 gctgagcaaa atatttcgtt cacgcgccat atgagctccg gcaacactac agacaacggc
    20281 atctttggcc tgttctatgg catctcgccg agctatatgg acggcattct gtcgacccgt
    20341 acgcctgcgg cattaattac tgcgcttaat cagcaaggct atcagctggg gttattctca
    20401 tcagatggct ttaccagccc gctgtatcgc caggcattgt tgtcagattt ctcgatgccg
    20461 agcgtacgca cccaatctga cgatcagacc gccacgcagt ggatcaactg gctgggccgc
    20521 tacgcacaag aagataaccg ctggttctcg tgggtttctt tcaatggcac taacattgac
    20581 gacagcaatc agcaggcatt tgcacggaaa tatagccggg cggcaggcaa tgtcgatgac
    20641 cagatcaaac gcgtgctcaa tgcactgcgt gattctggca aactggacaa tacggtggtg
    20701 attatcactg ccggtcgggg tattccactg agcgaagagg aagaaacctt tgactggtcc
    20761 cacggtcatc tgcaggtgcc attagtgatt cactggccag gcacgccggc gcagcgtatt
    20821 aatgctctga ctgatcatac cgatctgatg acgacgctga tgcaacgcct gctgcatgtc
    20881 agcacacctg ccagcgaata ttcgcaaggt caggatttgt tcaaccctca acgccgtcat
    20941 tactgggtta ctgctgcgga taacgatacg ctggcaatta ccaccccgaa aaagacgctg
    21001 gtgctgaaca ataacggtaa ataccgcact tacaacttac gtggtgaaag agtgaaagat
    21061 gaaaaaccac agttaagttt gttattgcaa gtgctgacag acgagaagcg ttttatcgct
    21121 aactgattaa ttataaatca gttagcgaaa tatcttactt gcaatcggtg tggaaaacgg
    21181 tagtattagc agccacgagt cggcacgtag cgcagcctgg tagcgcaccg tcatggggtg
    21241 tcgggggtcg gaggttcaaa tcctctcgtg ccgaccaaaa atcccaagaa aaaaccaacc
    21301 cttacggttg gtttttttat atctgcaatt aattcgataa acagaccgtg acacatcaca
    21361 gcctgtttat tttctgttat cagaacgtcc agaccacacc cgcctgagta ttccacggcg
    21421 attctacccc tgcgccattg ccatagctga ctgacaaatg accgcttaac gtcggggtaa
    21481 acgatgacct tatcccagcc tgataaacgc cactggttcc agccacatca ttattaaatt
    21541 tgccatcgtc attaactttc acctggttag aatcggcgta ttcctgacgc acggcggctt
    21601 tcagccaggg ttccagcgtc gtaccgtttt gcaggtccat gtgatagctt accgccgttc
    21661 ccgcttcagc gcgtaatatc cgggtatttc ccacatccgc gcgcatgccg tttgataacg
    21721 tgtagtcctg accatctgtg gtaaagccgg taaaggccag atagggtcta acactccaca
    21781 atccgtcaac ccaacggaac ccgctctcaa catgagcacc cgcgccgtta ctattgtaat
    21841 cgccaaacgc tgttgcccca ttactcatct tgccatggat ggtgttggca aaacggtcaa
    21901 ctttcaccac cccatcaaca taggcaccgt tctgatgctc ccaaccggca taagccccca
    21961 gggtatagct atcgatatta ccttttccgc cgcgatcaaa accaatatca gaatgagagt
    22021 aaccaaagaa caagccgcga attgtactgc tttcttcacg ggagaaacgg ctatcgatac
    22081 cgagcgtcag gcccgtcaat gtttgctcaa aaccagctcc cgcatcagtg gtcacgttgt
    22141 tgcgggtgtt aattgccgaa ctccacatca ccgtatcgta actaacgcct tttacgctac
    22201 caagacgctc acgcacggtg tccagttctg catcaaatac cagcggttgt gcggctgcca
    22261 tgttcagcac atcagtggtt gaaggggtaa tttgcgcgcg attctctgcg aggctccagc
    22321 tatggttgcc attatccagc aaggtatatt catacgtacc gatatcaaca acgcctccgg
    22381 cattgcccaa cgtaaatgca gcatcaccgc cgcccgttgt taccagtgta aggctatctc
    22441 ctgctgccgg gctggcaccg gtgtccgtca cgaatatttt gaaatcacct gttgcctgac
    22501 cggtgacgtt gagctgatcg ctctgatgat tggccatatc ggtacgcata taaaaattgc
    22561 cgtttcccga cagggtattg gtggtcaacg taatgaaatt ttccgcactt gccgtcacag
    22621 atgaacgagt cactggctca gccatcataa tcacatttgc gccggacatc gcaaggttgg
    22681 ctaagttgag gcgagtgttt tggttcgtcg tagtgatatc ggcaatgatg gtccatgcgc
    22741 tattttcgag cgaaacgtcc gctttgctgg tatccacggc accatgcgtt ctaaaagaag
    22801 cgccctcaga aaccactacc ttacccacca acatagcggg tgcataattt atttgcggct
    22861 tcatgacctc aagaatgcca tcattccctc tgactgacac gttaaccatt gtgccagccc
    22921 agacattagc gcggccattg ttgattacca tattttcaga gatggctttt gaggaatacg
    22981 tgatacttcc attctggtct gatcgaccga gatcatatac cgcatttgcc tgcactttcg
    23041 taccagtatc ctttcccagc gattgcatag tggcatgctc atcaaggata gtgtcaatgg
    23101 cctgcgtgtc ctccataaca atgagcccgg aaccatcatc cagttcataa ttttttgaca
    23161 caccatcctt tatactaaat tggcctttac tgtttgtacc actcacttcc agcgcattcg
    23221 ttgagacaat taatttcccg cctgcttttt tatcaacgcc agttgcagtc ccgccatcca
    23281 taacctccag taagccgcca ctatccacag tagtattata agcgaagtca ttttcttcaa
    23341 ctcgcaaact tccgccgttt tccaacaaca tattattagc aataccattc ttgatatcga
    23401 actgaccgag acggtttgtt ccgaatacct ccatggcccg cgtggttgtt ttaatagcac
    23461 cgcctgtttt ctgatctacc gcaagcgcaa atcccccctc tttaacctcc agtagtcctc
    23521 ccttttcgat agcggcaaaa acggcccccc catctttttc aacgattaag cgcccgcctt
    23581 taatcgtggt atcattcgct gggccataaa cctgtagcac accattattg agggtagtct
    23641 ggtttgccgc gccacccatt tgaacaattt gataaccatc atttaatact gagtattctg
    23701 cggtaccatt catttcaatg tattgatacc ctccgttaat ttcagtatta ctacttacac
    23761 caaattcttt tatatgctgt tctccgccag gattgatttt cgtattattg gcggtcccat
    23821 aaaccagctg gatatctttt tccaggataa caccatcaac tgtctcacca tactcaactg
    23881 ttgatgccaa tgctatgggt gaatataacg ctgtagccac cagaacagat attgcggaac
    23941 gacaaagaga aacagaacca gattgatgca ttgagctttc atcctatgaa attaattgca
    24001 gttaaaagca ttgggtacag aaaataccca tagctccata cccggagtca gtttttaaaa
    24061 actgtttaaa gaaatgcaca agtattgtga ttgatttttt agttgttttt cttgatgaga
    24121 agctgatgca aaattccgtc tttataataa aaatgatgcc aaagcgaacg acaaggttgt
    24181 agttttcact acatgtccat acataaaatg gggtaacatt cacgcgcctg gtagcgttac
    24241 caacgctacg ctcaaacata atgattctaa taaaacctca ggagactact atgcctgaag
    24301 caacgccttt tcaggtgatg attgtggatg atcatccact tatgcgacgc ggtgttcgtc
    24361 agttactgga gcttgatcct ggctttgaag tggtcgccga agcgggcgaa ggcgcgagcg
    24421 cgatcgatct ggcgaataga ctggatatcg acgtgatctt gctggatctc aatatgaaag
    24481 gtatgagtgg cctggatacc ctcaatgcct tgcgcaggga tggcgttacc gcgcaaatta
    24541 ttatcctgac cgtatccgat gcctccagcg atgtctttgc gctgatagac gcaggcgcag
    24601 acggttatct gttgaaagac agcgacccgg aagtattgct ggaagcgatt cgtaccggag
    24661 cgaaaggcag caaagtcttt agcgaacgcg tcaatcagta cttacgtgaa cgtgaaatgt
    24721 ttggcgcgga agaagatccc ttcagcgtgc tgacggagcg cgagctggat gttctgcacg
    24781 agctggcaca ggggctgtca aataaacaga ttgcatcggt gttgaatatt tccgagcaga
    24841 cagtaaaagt acatattcgc aatctgctgc gtaaactcaa tgtccgctca cgcgtggcgg
    24901 ccaccattct gttcctgcaa caacgcgggg cacaataaaa atagcccgat ggatttatca
    24961 tcgggctgag atttatgaca aacgcaaaac tgcctgatgc gctacgctta tcaggcctac
    25021 gtggatcgat caatttattg aatttacaca attttgtagg ccggataagg cgttcacgcc
    25081 gcatccggca taaactaagc gcactttgtc atcagtctag cccgatggca tcaccatcgg
    25141 gcctcttttt atttactctc ctgcggcgac aaatgttgca tcgcctgcgc gatactacgt
    25201 tcaatcaccg cacggcgagt atcgttggca ggtaagagtt tcaacatcat ctcccacgcg
    25261 gcaacggctt cgccaaatcg ctgctgctca aaggcattaa acgcatacat gcttagcaca
    25321 cggatattgc tatggtccgt tctcaccagc tgacgtagca gttcaccgcc gaggcggttg
    25381 tcgttgggat cagatgaacg agtcaacgct tcagcgtatc ccagtgcggc atcgctgttc
    25441 ttcggatcga ggcgatacgc agtagcgtat gcatcggtgg cgatactggc gttacccagc
    25501 gccatgccaa cgcggcccaa cataatccag ccttctatat ctcccggatt tttttgcagt
    25561 tgagtacgca tccccagcgc aaggcgcgac atctcttctt cgttgagcgg atcggctttc
    25621 gggtccagcg ccctgtccag caacgccgga gcctgtgccg tggcctgctg ccagattttc
    25681 acctgctgat aattgccagt ctggtagtag ctgacgccag ccacaattaa cgccaccaca
    25741 atacccggca gataaacaac atatccggca cgcttacctt ccggcacgct ttgttcagga
    25801 aacgcttccg gcaccacgcg tacccgacgc cgcgagcggg cgtatatgac ccaaccgcca
    25861 atgccaatag ccactactgg cagcacccac agcagcacgg tcagcggcgt taacggcgga
    25921 tcgtaagtga cgaagttgcc gtaacgcgcc accatataat cgacaatctc ttttttactt
    25981 ttaccttcct gcatcagttc atacactttc tgacgcaggt cggtggcaat catcgagttg
    26041 gaatcggcaa tgctgttgtt ctggcatttc gggcagcgca gttcttcagt gagctgacgg
    26101 aactgttgtt cctgtgcttc atctttaaac tgcaacacgt cgatggtcgc cagcgctgag
    26161 ccggagatca tcagcatcag cacgcccaat aaaaacctca ttgtgcggcc tccttactgt
    26221 atttctccca cagcggcttg atctcttctt cccagacgcg aggattgaga tcgcccgcat
    26281 ggcgatagcg aatgataccg ttgccgtcaa taaggaaggt ttctggcgcg ccatagacac
    26341 cgagatccag ccctaacatg ccgtcgccat caaacaggct tagcgcgtaa ggattgccca
    26401 gctctttcag ccagctgatt gccttctggc gatcgtcttt atagttcatg ccgaccacgc
    26461 gaatgccctg cgctgaaagc tgattcagat attgatgttc cgcacggcag gtcggacacc
    26521 aagtcgccca gacgttaagc agtactggtt tgccctgagt cagcacatcc gcctgataaa
    26581 actgccccgg attgtccagt gattcgagac gaaacttcgg cacaggcttg ccaatgagcg
    26641 ccgattccag attggtcgga tcatcccctt cggcattacg cgccagctgc cacagcagcg
    26701 ccgcggcaat cgccaggaag ataatcaacg gaattaacaa tactttgcgc ttcatacggc
    26761 ctccggcgca gttttttgcg gactcacgcg cttacgatag cgaggatcaa acagacacag
    26821 cagtccgccc aacgccatca tcagcccgcc cgcccaaatc cagcgaacaa atggtttgta
    26881 gtaaagacgc acggcccacg cgccgttttc cagctcttca ccgagcgcgg cgtacaggtc
    26941 acgcgtgatg ccgccgtcaa ttgccgcttc ggtcatcatc gacccggcag tgttgtaata
    27001 acgtttttcc gcatacagca ccgtttccgg tttaccctct cgcgttacgc caatagtcgc
    27061 cacaccgccg cgccagttcg ggccagtcac ctctttgaca tcacggaagg tgaagcgata
    27121 ttcatgaata tcgacgctat cgccggactt catgcgcaca tcacgctcaa cgctataatt
    27181 ctggctaaag gcaatgccaa caattgtcac tgccagcccc aggtgagccg ccaccatccc
    27241 ccaataactg aaggtggttt tcgtgccgcg tgaaatacgc agcgcagctt ccgcagttgc
    27301 cagcaccgca atccagcagg ccattgccag gccgagcacc gtcatcgcca caactttgct
    27361 ttcgaacagc cacggcaaca gcagagacag caccagcgta gagatgaagg cgataatcaa
    27421 taaattgcgg attttacgtg ggcgatcgcg cccccagcgc accagaggac cgacaccaag
    27481 cagtagcgca aacggcacca tcagccaggt aaacatggtg ttgaagaacg gttcgccaat
    27541 cgaaatactg cccagtccaa gttgcttgtg caccagcggc agcagcgtcc ccagcaacac
    27601 caccagcatc gcggcgacca gcaaaacgtt gttcgctaac agcaaagatt cccgcgacca
    27661 cagcgcattg tttacgcgtg agcgaacttt gtgtccacgc gcggcaaaca gcagcagcga
    27721 accgccaatc accagcacca taaaggcgag gataaacata ccgcgcgccg gatcagacgc
    27781 aaacgcgtgt accgatacca gcacgccgga acgcacgagg aaagtcccca acagacacaa
    27841 cgagaaggca ctgattgcca gcagtaatgt ccacgctttg aagctggcgc gttgttcagt
    27901 gaccgccagt gagtgcatca gtgcagtccc caccagccac ggcataaacg aggcgttttc
    27961 taccggatcc cagaaccacc agccacccca gccgagttcg taataggccc atgcggaacc
    28021 gagcacaatg ccgagcgtta ggaaaatcca cgccgccagc gtccacgggc gagtaaaacg
    28081 cgcataagtg ctgtccagac gcccgctcag caaagaagca atggcaaaag caaacgccac
    28141 cgagaaaccc acgtacccca tataaagcag aggcggatgg aagatcagcc ccggatcctg
    28201 taacagcggg ttaagatcgc gcccttcaat cgggaagttc ggcagcgtgc gtgagaacgg
    28261 gttagaggta aagagaatga acagcaaaaa gccgacgctg accatcccca ttatcgccag
    28321 tacacgggcc acaatatcca gcggaatacg ctgactaaaa atcgccaccg caaaggtcca
    28381 gccgctcatc agcagcaccc acagcagaag cgaaccttca tgcgcgcccc aagtagccgc
    28441 cacgcgatac cacaccggaa gctgggtatt ggagttgctg gcaacatagg tgacggtaaa
    28501 gtcgttgacc acgaaagcat tgaccagcac cagaaatgcg ccagccacag acataaacag
    28561 cagccaggca aacaagcggg aagacgccat catgcgcgca tctccgcgcg ccacgcccca
    28621 tagcggatac acggacagca gcagcgcaat ccctaacgcc aagcacagca gcccgtttcc
    28681 aatttccggc atcatgatgc tgggtcctta taaacactcg ccgggcgacg gtggttagct
    28741 tccatcgctt tctcaacttc tggcggcgtg tagttttcgt cgtgtttcgc cagcacttct
    28801 ttcgcgagga tatgattgcc tttttccagc tcgccctgca ccacaacgcc ctgcccttca
    28861 cggaacagat ccggcaaaat gccttcgtaa gagacatcca ctgagccttc agcatcgtaa
    28921 atggtgaagg tcactttcag cgaattggga tcgcgctgca cgctacccgg catcaccatc
    28981 ccgccaacgc gcagacgctg accgacttcc ggcatctgct gagtttcacg cttgccgtag
    29041 agaatttccc ccggcgtata aaagagatcg atattcgagc gcagcgcata tagcaccaga
    29101 ccgatagtca gcgctagccc tgccaacacg gcacaggcaa tccacaagcg gtttttacgg
    29161 cgaatattca tgcagcctcc tgctgttgcg cagcacgtaa acgcgcctca cgcgcccgct
    29221 gttgcgccac accacgcaga attgcgcgat gttgcatcac cgagtgcacg accaaaacca
    29281 ccagcggaat aacggtcatc accaccgcca gccagacaaa aaaggcgtaa ccgcccattg
    29341 cgaaaaattc attccaggaa gcaaatgcag gggtcattta cggcctcttt tcagtatcag
    29401 ttcactcacc cacggacggc gtttttccat cagcaaaatc aaattacgca tccgcatcag
    29461 cgtcagcgtg gcagacagga gcaggaagcc aaaaatcgac cagcgcagcg gcgaacgcat
    29521 cgccggatcg atactttgct gcatccgcgt tgatccctga tgcagggtgt tccaccactc
    29581 cacggagtaa tgaataatcg gcagattcac cacgccaatc agcaccatga tacctgccgc
    29641 acggcccgcc agacggcggt cgtcgaaggc gtgccacagg gcaatcacac ccacatacaa
    29701 aaacagcagc accagttcag aagtcagacg tgcatcccat acccaccagg tgccccacat
    29761 cggttttccc catgcagagc cggtaaccag ggcaataaag gtaaacacgg caccaatggg
    29821 ggccatcgcc gccaccgcca ggttggccat tttcatctgc cagacaaggc caataaacgc
    29881 cgccaccgcc attgatgcat aaatgcccat cgaccagatc gccgcaggca catgcaggta
    29941 gataatgcgg tagctatttc cctgctgata atcagccgga gcaaagccga atccccagat
    30001 ccagccgacg gtaagcacga ccacactggc aattgccagc cacggtataa accagccaca
    30061 gatttgatac agccgtggtg ggatcgccag ttgatgcagt gttttccaca tagtttcgat
    30121 accagactcg aacaaaaatc agtaatccag cgttattgaa tgctgattcg taacgctgcc
    30181 gccgtcgcaa aaggacttaa tgtcgcggtg cctgccagca acgcgcctaa aattgccaga
    30241 tacccatcaa cgggcaaatg catagaagcc gcgtccatcg cggcggtggc aaagatgagt
    30301 aatgggatag tcagcggtaa caccagtatg ctgagcagca caccaccgcg cttaagtccc
    30361 actgtcagcg ccacgcccgg tgcaccgaga aagccaagcg taggcgttcc cagcagcagc
    30421 gtcagcgcca tcacttgcca gccataaaca tccattccca gtagcattgc taccagtggc
    30481 gaaaggatga gtaacggcag accggttacc atccagtgcg ccatcacctt cgccagcaca
    30541 acggcgggca agggtaacgg caacaacatc aattgttcaa gactgccgtc ctgcaaatcg
    30601 tcacggaaca gtcgttccag cgccagcaag gatgaaagca gcgcagcaac ccagataatg
    30661 cccggtgcaa tacgcgccag cagttgcggc tccggaccga tactgagcgg aaaaagggta
    30721 attacaatca ggaagaacca cagcgggttg gcgatttcgg cgctatggcg aaacgctacg
    30781 cgcagttcaa gacggaaaat acgccagaac atcatgcggc cctcgtttgc gtcagtgaaa
    30841 tgcggcgaat tttactttca gcaacgttga gcggctggtg ggtagtcaga atcacaatcc
    30901 ccccctgctc cgtatgctgc gccatacgct gggtcagacg atcgacgccg ttaacgtcaa
    30961 tcgcggtaaa aggctcgtcg aggatccata atgtggcacg ggtcaaccac agacgcgcta
    31021 aagcgacgcg gcgttgttgc ccggcagaaa gttgatttac cggaatatct tcgaatccgg
    31081 caagcccggc ctgcgccaag gcttccagac attgtgcggc atcgccatcg cgatgataaa
    31141 agtgcagatt ttctaacgcc gtcagccggg ttttgatccc cggctgatgg cctatccata
    31201 acagggtttg atggtagctg tcgcgtacct gatgcaaggg ctgcccttgc cagagaacct
    31261 cgccagcgtc agggcgagac aaccccgtca gcaaacggag aagcgttgtc ttccccgcgc
    31321 cgttgctacc ggtgatttgt acccactctc ctgcgttcag cgtaaatgac aagccactaa
    31381 ataaggttcg ttcatcccgc tcacaaagta actctctggc ttcaagcata cccacgcaat
    31441 aaccctgtta aaaacctggc tcgacttcac gcatatcggg cagcttgtgc gcgatccctt
    31501 tatggcaatc aatacaggtt tgcccatctt tcaccgcctg gtcatgcatc ttcgcggcaa
    31561 ccgatttctg ggcggttgta tccatatact cgaagttgtg acagttacgg cactcctgcg
    31621 agttattgtc cttcatgcgc cgccactcat tctgtgccat cgtcagacga tgagcttcaa
    31681 atttctgcgg cgtgtcaata acgccaaaaa ttttaccata cagctcttta cttgctttga
    31741 gcttgcgtat catcttcggc acaaactcgt gcggaacgtg acaatccgga caggtcgcac
    31801 ggacgccgct acggttgttg tagtgcacgg aatccatgta ttcctgatac accgtgttgc
    31861 gcatttcgtg gcagctaatg cagaactctt cggtattggc tttttccatc ccggtgttaa
    31921 agccccccca gaagacgatg ccgccaacaa aaccgatcaa cagcagcgtc cccagcgcca
    31981 gatggctggg ggtacgccac catttccaca ggcgcttaat cagaccaggc ttacggtcag
    32041 aatttcccat aataacctct tatttcccgt aaccttttga tggggtaaag gtattcccca
    32101 cgattggcgc ggtatcggcc tgcggtacgt gacattgcag acagaaataa cgacgcggag
    32161 ccacttccgc gcccactttg ccgtcgctgt ccataaagtg agtaggactg atacgcggcg
    32221 cgccagtggt gcgatagctt tcgacaccgt ggcactgcaa gcagcgattg gtattggtcg
    32281 ttacctgata accttcaacg ctatgcggga tcattggcgg ctgattcaca tagttcagcg
    32341 gcatccgatc ctgctctttt ggcatccgaa tggccccttc ctgcgtcccg gatacttccg
    32401 gcgactggct aaaatcgacg ccattagccg cccaaaccgc gccgcttacc accagggcca
    32461 gcatcgccgt ccattgacac agcgctttct tcaggtcatg gcttttcatg atttcgctcc
    32521 cgaactccat cgtgtagtta ttgtaaatac atcctcagaa cagacatcca cgcagcgacc
    32581 gcacgtcatg caatcgcggc tggtgacctg caccgggctt tgttcatcca gcaccggggc
    32641 acgtagcaca tgcggttccg ggcaaacatg aaaacaatcc atacagcggt tacatttctg
    32701 ccgatcgctg gccgcaacgg taatcacacc tttgctaccc agcacgccat acagcgcgcc
    32761 aaccggacaa atgtgcccgc accagccgtg ttcaacgacc agtagatcaa ataaaaacag
    32821 cgcgagaata agcagcgcgc cgctgccgaa gcccattacc aggctacgcc ccatcagaga
    32881 aacggggttg atccattccc aaatgagcgt gccagttaag gctgatccca ccagaatgac
    32941 caccaacagc acgtagcgaa tgtggcgagg gatcgtcgca gactgattga ggtcaaagcg
    33001 cctgcgtaac cagttcgcta agtcggtaat cgggttcagc gggcagaccc agctgcaaaa
    33061 taatcgcttt cccgccaggg cgtagagcac ggtgataatc accgcaccgg tcaacgccac
    33121 cgtggcaggc agatgaccac tggcaagact ttgcagcgtc atcagcggat cggtcagcgg
    33181 aacggtgtcg aataacaggc tactgctgta gttgccgtgc aagatccaca caccaaacca
    33241 tggaccgctc aaaaacatcc ccagcacgaa gaactgacaa aggcgacgta acaccagcca
    33301 acggtgactg cgccaccagc cttttttctc cagcgcctcg cgcccggcgt cacgtttacg
    33361 atttgccatt gttcccctcc agccagccga agcggtaatg gtgacctaac tcccctttcg
    33421 ccagtgacag cggtaacacc ttgattgccg gttgttccag cacgcatact ttttcgcact
    33481 taccgcaacc ggtacaggcg tcgctgtgaa ccgtcggcag aaagcgggcg tgcttaccgg
    33541 tacgcgtgtt gcgctccagc tccaaggtga tggcctcatc aattttcggg cattcgcgat
    33601 aacaaacatc gcagcgcagc ccctgaaagt tgagacagtt ttcctggtcc accagtaccg
    33661 ccagccccat ccgcgcgtcg tcgatcgatt caatctcacg atccagcgca ccgcttgggc
    33721 acactttggc gcacggaatg tcctcacaca tttcgcaagg aatatcccgt gcgacaaaat
    33781 atggcgtgcc cgccgacaga ccagaggcca gcgtcgccag ttttaaggtg tcgtaagggc
    33841 aagcctgaac acactgaccg caacgcacac aggcactggc aaaggcgttc tcgtttatgg
    33901 ctccgggcgg gcgcaaccgc acgccagatg cgcgtgcggt ttgctgttgt aaccccagcg
    33961 ccacacccac ggcagccagc ccgcctgctg tgcgaacgac atcgcgcaga aagcggcggc
    34021 gaccattttg aggtttcgct gaccgggaca taatggctta caccttctcc agtttgaccg
    34081 cgcacttctt gaagtccgtc tctttcgaga gcggatcggt cgcatccagc gtcagtttgt
    34141 taaccagctg tgcggcgtcg aagaacggca tgtacaccag gccctgtggc ggacggttac
    34201 gaccgcgcgt ttcaacaatc gagatcactt cgccacggcg agaaaccact ttcactttgt
    34261 cgccacggcg cagatcgcgc gctttcgcat ccagcgggtg aataaacaag accgcttccg
    34321 ggaaggcgcg gtgcagttcc ggcacacggc gagtcatact gccggtgtgc cagtgctcca
    34381 gaacgcgtcc ggtagagagc cacaggtcgt actcttcatc cggtgcttcc gccgccggtt
    34441 cgaacggcag tgcgaagatc accgctttgc catccggttt accgtagaac ttatagcctt
    34501 cgcccgcttt cacgtacggg tcgttacctt cgctgtaacg ccactgtgtt tctttaccat
    34561 tcaccaccgg ccagcgcaga ccgcgcgctt tgtggtagtc atcgaacggt gccagatcgt
    34621 gaccgtgacc gcgaccaaac catgcgtact cttcgaacag ccctttttgc agatagaagc
    34681 ccagctcgcg ggattcatcg ttcagctgat cttccgccag ttcggatacc gggaatttgc
    34741 tcacttcggg ggtggcatac agaacttcgt acagcgtttt gccacgcagt tccggtttct
    34801 tcgccagcag ctcttccggc catacttctt cagttttgaa gcggcgggag aactggacta
    34861 actgccagag atccgatttc gcttcgcctg gcgcctgtac ctgctgacgc cagaactgag
    34921 tacggcgttc ggcgttaccg taagcgccct ctttctctac ccacattgcg gtcggcagga
    34981 tcaagtcggc ggccagcgca ctgactgtcg gatacggatc ggagacgatg atgaagttgc
    35041 gcggatcgcg ccagcccggc atacgctctt cattgatgtt cggcccggcc tgcatgttgt
    35101 tggtacacat ggtccagtaa acattcagct tgccgtcttt cagcgcacgg tcttgtgcca
    35161 ccgcatgaag accgattttc gccggaatgg tgccgctcgg gatattccac ttcttctcac
    35221 agatatcacg atgtttctcg ttagttacca ccatgtccgc aggcagacgg tgagcaaagg
    35281 tgcccacttc acgcgcagta ccacacgcag aaggttgccc ggtcagggag aacggaccgc
    35341 aacccggctg ggaaattttg ccggtcagca ggtgcaggtt gtagaccagg ttgttagccc
    35401 acacaccacg ggtatgctgg ttgaagccca tcgtccagta ggagatgact ttcttgttcg
    35461 gatcggcata cagctgcgcc agttgttcta actggtcttt cggcacgccg gtcatttcag
    35521 cggttttttc cagcgtatac tcagcaacga aggctttgta atcttcaaag ctcatcggtt
    35581 cggaggcgtc agaacccgga ttcttcgctg ctttttccag cggatgggtc ggacgtaaac
    35641 cgtagccgat gtccgtcgcc cctttgcgca ggttaacgtg cttgctgaag aagtcctgat
    35701 ttatcgcatt gttttgaatg atatagttgg cgatgtagtt caggatcacc aggtcagatt
    35761 gcggcgtaaa gatgatgccg ttatccgcca gctcgaagct acgatgctgg taggtagaaa
    35821 gcaccgccac ggtgacgttc tggttagaga gacgactgtt agtgatgcgt gaccagagga
    35881 tcgggtgcat ctccgccatg ttagcgcccc acagcacaaa cgcgtcagcc tgctcgatgt
    35941 cgtcatagca gcccatcggc tcatccatac caaaggtacg cataaagcca actactgccg
    36001 acgccataca gtgacgtgcg ttcgggtcga tgttgttcga acggaagccc gctttgaaca
    36061 gcttggacgc ggcataacct tcccagatag tccactgacc agaaccgaac ataccgatcg
    36121 attccggccc tttttctttc agggcggttt tgaatttctc ttccatcaca tcgaaggcct
    36181 gatcccaggt gattggggta aattcgcctt ctttgtcata tttaccgttt ttcatacgca
    36241 gcagcggctg cgtcaaacgg tctttaccgt acatgatctt gggcaggaaa tagcccttaa
    36301 tgcagttcag gccacggtta accggtgcgt ccgggtcgcc ctgacaggcc accacacgcc
    36361 cctgctgcgt tccgaccaga acgccgcaac cagtaccgca gaaacggcac ggcgctttat
    36421 cccatttaat ggcttcctgc tgaccaacaa cggcgcgggc aacgcccggc acgctgagac
    36481 cggcagccgc cgcagcggcc gcaacggcgt tagctttcat aaagctacga cgactgagtt
    36541 tcatggtgtt tcctcacctt gctcttcctg ctggtgataa accagcgaca ccgccagcac
    36601 gccctctacg ttgcgtactg actcaatggt ttggatcagc gtttcgctgt cttctgcttc
    36661 caccaccaca atcaactgac cgctcggcgc gtcgctgaca gcaacttcac agccgggaaa
    36721 ggcgttcagt tgggtgctga tgtctgaaat tcgttcgctt ttggcctgca cgaccaggct
    36781 gcaaacttgc cagttagtgt gcatggagat actccgcagt tatggctgat accggacagc
    36841 tggctgcaca tgctccacat ccgttgcaaa gttggctatt aagttgcgcc tggtagatcc
    36901 cggagagcgt cgggcgaaag ataattgcca ttggctcaca gctatcctga cagcggcggc
    36961 attcaactga ctgatacgcc aggcacgcgt ccccgatggt gaactgcaaa tcccaggccc
    37021 tggtgtggcg cggagaaaat agcgattcgg ggcaagcctg cgcgcaggcg taacagaagc
    37081 tgcactcgtt atttttgaaa ttaacgctcg gatagccacc cgtgccgcgt tgcagaatgt
    37141 tgttttcaca agcattaata caggcgtcgc agcgggtgca atgggtcaga aaatgagatt
    37201 catcaccgga ccagggcgga cggataccgt tactggcttt gcgccagcaa ccagtgagta
    37261 tgccccgacg ggatgcatca atcttcacat tgaccttcca tcattaacgc gctctgaaaa
    37321 ttgagagcga ccaaatagac cgcataatta ataagccatt tttatagccg ctaagatatt
    37381 aaaggatgtg tcaaagatgc ataccccgat cggggtaaaa cctgtagcag gatcaaaaga
    37441 gtggcgggaa gcgtggcaaa aacgggcttt tgctcacatt tcaaatggtt ataaacatat
    37501 ttatatagcg attaattcac cagag
//
LOCUS       DAZPAB010000018        34986 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZPAB010000018 DAZPAB010000000
VERSION     DAZPAB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34986
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.018"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..805)
                     /locus_tag="ACHUPG_000834"
     CDS             complement(<1..805)
                     /locus_tag="ACHUPG_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0860556.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQR"
     gene            897..983
                     /locus_tag="ACHUPG_000835"
                     /pseudo
     CDS             897..983
                     /locus_tag="ACHUPG_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(993..1073)
                     /locus_tag="ACHUPG_000836"
                     /pseudo
     CDS             complement(993..1073)
                     /locus_tag="ACHUPG_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1388..1864)
                     /locus_tag="ACHUPG_000837"
     CDS             complement(1388..1864)
                     /locus_tag="ACHUPG_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR0860557.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1923..3212)
                     /gene="bioA"
                     /locus_tag="ACHUPG_000838"
     CDS             complement(1923..3212)
                     /gene="bioA"
                     /locus_tag="ACHUPG_000838"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR0860558.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3299..4339
                     /gene="bioB"
                     /locus_tag="ACHUPG_000839"
     CDS             3299..4339
                     /gene="bioB"
                     /locus_tag="ACHUPG_000839"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR0860559.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4336..5490
                     /gene="bioF"
                     /locus_tag="ACHUPG_000840"
     CDS             4336..5490
                     /gene="bioF"
                     /locus_tag="ACHUPG_000840"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR0860560.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5477..6232
                     /gene="bioC"
                     /locus_tag="ACHUPG_000841"
     CDS             5477..6232
                     /gene="bioC"
                     /locus_tag="ACHUPG_000841"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR0860561.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6225..6902
                     /gene="bioD"
                     /locus_tag="ACHUPG_000842"
     CDS             6225..6902
                     /gene="bioD"
                     /locus_tag="ACHUPG_000842"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR0860562.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7481..9502
                     /gene="uvrB"
                     /locus_tag="ACHUPG_000843"
     CDS             7481..9502
                     /gene="uvrB"
                     /locus_tag="ACHUPG_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR0860563.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9694..10602)
                     /gene="yvcK"
                     /locus_tag="ACHUPG_000844"
     CDS             complement(9694..10602)
                     /gene="yvcK"
                     /locus_tag="ACHUPG_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR0860564.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10867..11011
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10999..11988
                     /gene="moaA"
                     /locus_tag="ACHUPG_000845"
     CDS             10999..11988
                     /gene="moaA"
                     /locus_tag="ACHUPG_000845"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR0860565.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12010..12522
                     /gene="moaB"
                     /locus_tag="ACHUPG_000846"
     CDS             12010..12522
                     /gene="moaB"
                     /locus_tag="ACHUPG_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR0860566.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12525..13010
                     /gene="moaC"
                     /locus_tag="ACHUPG_000847"
     CDS             12525..13010
                     /gene="moaC"
                     /locus_tag="ACHUPG_000847"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR0860567.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13003..13248
                     /gene="moaD"
                     /locus_tag="ACHUPG_000848"
     CDS             13003..13248
                     /gene="moaD"
                     /locus_tag="ACHUPG_000848"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR0860568.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13250..13702
                     /gene="moaE"
                     /locus_tag="ACHUPG_000849"
     CDS             13250..13702
                     /gene="moaE"
                     /locus_tag="ACHUPG_000849"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR0860569.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13839..14543
                     /locus_tag="ACHUPG_000850"
     CDS             13839..14543
                     /locus_tag="ACHUPG_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR0860570.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14748..15461
                     /locus_tag="ACHUPG_000851"
     CDS             14748..15461
                     /locus_tag="ACHUPG_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR0860571.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15497..16453)
                     /locus_tag="ACHUPG_000852"
     CDS             complement(15497..16453)
                     /locus_tag="ACHUPG_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFR0860572.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16453..17694)
                     /gene="clsB"
                     /locus_tag="ACHUPG_000853"
     CDS             complement(16453..17694)
                     /gene="clsB"
                     /locus_tag="ACHUPG_000853"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR0860573.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17691..18452)
                     /locus_tag="ACHUPG_000854"
     CDS             complement(17691..18452)
                     /locus_tag="ACHUPG_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR0860574.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18585..18995
                     /locus_tag="ACHUPG_000855"
     CDS             18585..18995
                     /locus_tag="ACHUPG_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR0860575.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18957..20063)
                     /locus_tag="ACHUPG_000856"
     CDS             complement(18957..20063)
                     /locus_tag="ACHUPG_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0860576.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVLFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20074..21207)
                     /locus_tag="ACHUPG_000857"
     CDS             complement(20074..21207)
                     /locus_tag="ACHUPG_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0860577.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21200..22936)
                     /locus_tag="ACHUPG_000858"
     CDS             complement(21200..22936)
                     /locus_tag="ACHUPG_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0860578.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22929..23924)
                     /gene="hlyD"
                     /locus_tag="ACHUPG_000859"
     CDS             complement(22929..23924)
                     /gene="hlyD"
                     /locus_tag="ACHUPG_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR0860579.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23927..24598)
                     /gene="cecR"
                     /locus_tag="ACHUPG_000860"
                     /pseudo
     CDS             complement(23927..24598)
                     /gene="cecR"
                     /locus_tag="ACHUPG_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
     gene            24827..26047
                     /gene="rhlE"
                     /locus_tag="ACHUPG_000861"
     CDS             24827..26047
                     /gene="rhlE"
                     /locus_tag="ACHUPG_000861"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR0860580.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSVAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGIRLMNKRKTA"
     gene            complement(26041..26139)
                     /locus_tag="ACHUPG_000862"
                     /pseudo
     CDS             complement(26041..26139)
                     /locus_tag="ACHUPG_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26221..26703)
                     /gene="ybiA"
                     /locus_tag="ACHUPG_000863"
     CDS             complement(26221..26703)
                     /gene="ybiA"
                     /locus_tag="ACHUPG_000863"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR0860581.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26823..28973
                     /gene="dinG"
                     /locus_tag="ACHUPG_000864"
     CDS             26823..28973
                     /gene="dinG"
                     /locus_tag="ACHUPG_000864"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR0860582.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29001..29963
                     /gene="ybiB"
                     /locus_tag="ACHUPG_000865"
     CDS             29001..29963
                     /gene="ybiB"
                     /locus_tag="ACHUPG_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR0860583.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30104..31189
                     /gene="hcxB"
                     /locus_tag="ACHUPG_000866"
     CDS             30104..31189
                     /gene="hcxB"
                     /locus_tag="ACHUPG_000866"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR0860584.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            31256..31315
                     /gene="ybiE"
                     /locus_tag="ACHUPG_000867"
     CDS             31256..31315
                     /gene="ybiE"
                     /locus_tag="ACHUPG_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR0860585.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(31418..31678)
                     /gene="ybiJ"
                     /locus_tag="ACHUPG_000868"
     CDS             complement(31418..31678)
                     /gene="ybiJ"
                     /locus_tag="ACHUPG_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR0860586.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASPLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31943..32209)
                     /gene="ybiI"
                     /locus_tag="ACHUPG_000869"
     CDS             complement(31943..32209)
                     /gene="ybiI"
                     /locus_tag="ACHUPG_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR0860587.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32283..32960)
                     /gene="ybiX"
                     /locus_tag="ACHUPG_000870"
     CDS             complement(32283..32960)
                     /gene="ybiX"
                     /locus_tag="ACHUPG_000870"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR0860588.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33002..34986)
                     /locus_tag="ACHUPG_000871"
                     /pseudo
     CDS             complement(33002..34986)
                     /locus_tag="ACHUPG_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8292 a         8970 c         9450 g         8274 t
ORIGIN      
        1 atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata ttaacatctg
       61 cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg ttagggatat
      121 atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt tccccaacgt
      181 ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta tcaaaagcac
      241 ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg ttgccaacag
      301 aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg agaagggctg
      361 agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg ttaagtgaaa
      421 catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg ttatcgctgg
      481 ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca ttgctagaga
      541 tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg agtaaactta
      601 aattgagggc tggctcttga aaagccagac atattttcag taatcgaact gcctgtgttc
      661 ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga taatccgtga
      721 tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag gagcggaaaa
      781 aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata tattaatata
      841 ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt taagaacagg
      901 aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg ctcaatgtgc
      961 tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca gtagtctgcc
     1021 caccacagca tcatttctct gcgcttatcg agatactgag catggttgta aattcagcta
     1081 acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg cgctaatgct
     1141 ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga tatgttgtgt
     1201 tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat tgatattaat
     1261 gatttttact gtttctcggt cagctttttt atactaactt gagcgaaacg ggaaggtaaa
     1321 aagacaaaaa gttgttttta atacctttaa gtgataccag atggcattgc gccatctggc
     1381 agagtgatta actaaacatc gcggtaatcg atgcgcttgc cagagagtgg aaatgaacgt
     1441 taaacccgac catcgcgccg ctggctcctt catcgacatc aatacgctct acatccagcg
     1501 cgtgaacggt aaaaatgtag cgatgggttt cgcctttcgg cggcgctgcg ccatcgtatc
     1561 cggttttacc aaaatcggta cgcgtctgca aaacgccgtc tggcattgct accagaccag
     1621 agccaaaccc ttgcggtaat acgcgggtat cagcgggtaa attaacaact acccagtgcc
     1681 accagccgga gccggttggc gcatccgggt cgtagcaggt gacaacaaaa cttttcgttc
     1741 ccgcaggaac atcatcccac gccagatgcg gtgaaatatt atcgccatcg taacccatgc
     1801 cgttaaagac atgacgatgc ggcaacttat cgccatcgcg cagatcgtta ctgatgagtt
     1861 tcatgaaccc tcctttcttg tttgcagaaa gtgtagccag aaaccctcac gctgacttcc
     1921 cgttattggc aaaaaaatgt ttcatcctgt accgcgcggt taaccgctgc ggtcagacgc
     1981 tgcaactgtt gcgggagaat aatatagggc ggcatcaggt aaatcagttt gccaaaaggc
     2041 cggatccata ccccctgttc gacaaagaat ttttgcagcg ccgccatatt caccggacga
     2101 gtggtttcga ccacaccgat tgcccccagt acgcgcacat cggcaaccat ttcggcatca
     2161 cgggctggtg ccagttgctc gcgcagctgc acttcaatag ccgccacctg ctgctgccat
     2221 tcgccggatt cgataatcgc taggctggcg tttgctgccg cgcaggccag cggattgccc
     2281 ataaaagttg gcccatgcat aaagcagccg gcttcgccgt tactgatggt ttctgcaacc
     2341 tcgcgcgtgg taagtgtggc ggaaagggtc attgtgccgc cggttaaggc tttaccgagg
     2401 cacaaaatgt ccggcgcgat ttctgcatat tcacaggcaa acagtttgcc ggtacgacca
     2461 aatccggtgg cgatctcgtc ggcaatcagc aagatacctt cgcgatcgca cattttgcgg
     2521 attcgtttta accattccgg atggtacatg cgcatcccgc ctgcgccctg gacaatcggc
     2581 tcaatgatca ccgctgcgat ttcatgacga tgcgccgcca tcaggcgggc aaaaccaacc
     2641 atatcgcgtt catcccattc accatccatg cggctttgcg gggcgggagc aaacaagttt
     2701 tctggcagat agcctttcca caggctgtgc attgagttgt ccggatcgca caccgacatc
     2761 gcgccaaagg tatcgccatg ataaccattg cggaaggtca gaaaacgctg gcgcgcttcg
     2821 cctttggctt gccagtactg caacgccatt ttcatcgcca cttccaccgc tacggaaccg
     2881 gagtccgcga gaaaaacgca ctccagcggt tgcggcgtca ttgccaccag tttgcggcat
     2941 agctcgatgg cgggcgcatg ggtgataccg ccaaacatca catgcgacat ggcatcaatt
     3001 tgcgtcttca tcgccgcatt aagctgcggg tgattgtagc cgtggatcgc cgcccaccag
     3061 gacgacatac cgtcaaccag gcgtctgccg tcagacaaaa ttagctcgca accttcggcg
     3121 ctcgccaccg gataaaccgg cagaggggag gtcatggatg tgtatgggtg ccagatatgg
     3181 cgttggtcaa aggcaagatc gtccgttgtc ataatcgact tgtaaaccaa attgaaaaga
     3241 tttaggttta caagcctaca ccgaattaac aacaaaaaac acgttttgga gaagccccat
     3301 ggctcaccgc ccacgctgga cattgtcgca agtcacagaa ttatttgaaa aaccgttgct
     3361 ggatctgctg tttgaagcgc agcaggtgca tcgccagcat ttcgaacctc gtcaggtgca
     3421 ggtcagcacg ttgctgtcga ttaagaccgg agcttgtccg gaagattgca aatactgccc
     3481 gcaaagctcg cgctacaaaa ccgggctgga agccgagcgg ttgatggaag ttgaacaggt
     3541 gctggagtcg gcgcgcaaag cgaaagcggc aggatcgacg cgcttctgta tgggcgcggc
     3601 atggaagaat ccccacgaac gcgatatgcc gtacctggaa caaatggtgc agggggtaaa
     3661 agcgatgggg ctggaggcgt gtatgacgct gggcacgttg agtgaatctc aggcgcagcg
     3721 cctcgcgaac gccgggctgg attactacaa ccacaacctg gacacctcgc cggagtttta
     3781 cggcaatatc atcaccacac gcacttatca ggaacgcctc gatacgctgg aaaaagtgcg
     3841 cgatgccggg atcaaagtct gctcaggtgg cattgtgggc ttaggcgaaa cggtaaaaga
     3901 tcgcgccgga ttattgctgc aactggcaaa cctgccgacg ccgccggaaa gcgtaccaat
     3961 caacatgctg gtgaaggtga aaggcacgcc gcttgccgat aacgatgacg ttgatgcctt
     4021 tgattttatt cgcaccattg ccgtagcgcg gatcatgatg ccgacctctt acgtgcgcct
     4081 ttctgccgga cgcgagcaga tgaacgaaca gactcaggcg atgtgcttta tggcaggcgc
     4141 aaactcgatt ttctacggtt gcaaactgct gaccacgccg aatccggaag aagataaaga
     4201 cctgcaactg ttccgcaaac tggggctaaa tccgcagcaa actgccgtgc tggcagggga
     4261 taacgaacaa cagcaacgtc ttggacaggc gctgatgacc ccggacaccg acgaatatta
     4321 caacgcggca gcattatgag ctggcaggag aaaatcaacg cggcgctcga tgcgcggcgt
     4381 gctgccgatg ccctgcgtcg ccgttatccg gtggcgcaag gagccggacg ctggctggtg
     4441 gcggatgatc gccagtatct gaacttttcc agtaacgatt atttaggttt aagccatcat
     4501 ccgcaaatta tccgtgcctg gaagcagggg gcggagcaat ttggcatcgg tagcggcggc
     4561 tccggtcacg tcagcggtta tagcgtggtg catcaggcgc tggaagaaga actggccgag
     4621 tggcttggct attcgcgggc gctgctgttt atctctggtt ttgccgcgaa tcaggcagtc
     4681 attgccgcga tgatggcgaa agaggaccgt attgttgccg accggcttag ccatgcctca
     4741 ttgctggagg ctgccagttt aagcccgtcg cagcttcgcc gttttactca taacgatgtc
     4801 gctcatctgg cgcgactgct tgcttccccc tgtccggggc agcaactggt ggtgacagaa
     4861 ggcgtgttca gcatggacgg cgatagcgcg ccactggcgg aaatccagca ggtaacgcaa
     4921 cagcacaatg gctggttgat ggtcgatgat gcccacggca cgggcgttat cggggagcag
     4981 gggcgcggca gctgctggct gcaaaaggta aaaccagaat tgctggtagt gacttttggc
     5041 aaaggatttg gcgtcagtgg ggctgcggtg ctttgctcaa gtacggtggc ggattatctg
     5101 ctgcaattcg cccgccacct tatctacagc accagtatgc cgcccgctca ggcgcaggca
     5161 ttacgtgcgt cgctggcggt cattcgccgt gatgaggggg atgcacggcg cgaaaaactg
     5221 gtgtcactca ttgcgcgttt tcgtgccgga gtacaggatt tgccgtttac gctggctgat
     5281 tcatgcagcg ccatccagcc attgatcgtc ggtgataaca gccgtgcgtt acaactggca
     5341 gaaaaactgc gccagcaagg ctgctgggtc acggcgattc gcccgccaac cgtacccgct
     5401 ggtactgcgc gactgcgctt aacgctaacc gctgcgcatg aaatgcagga tatcgaccgt
     5461 ctgctggagg tgctgcatgg caacggttaa taaacaagcc attgcagcgg catttggtcg
     5521 ggcagccgca cactatgagc aacatgcaga tctacagcgc cagagtgctg acgccttact
     5581 ggcaatgctt ccacagcgta aatacaccca cgtactggac gcgggttgtg gacctggctg
     5641 gatgagccgc cactggcggg aacgtcacgc gcaggtgacg gcgttagatc tctcgccgcc
     5701 aatgcttgcg caagcccgcc ataaagatgc cgcagatcat tatctggcag gagatatcga
     5761 atccctgccg ttagcgactg cgacgttcga tcttgcatgg agcaatctcg cggtgcagtg
     5821 gtgcggtaat ttatccacgg cactccgcga gctgtatcgg gtggtgcgcc ccggaggcgt
     5881 ggtcgcgttt accacgctgg tgcagggatc gttacccgaa ctgcatcagg cgcggcaggc
     5941 ggtggacgag cgtccgcatg ctaatcgctt tttaccgcca gatgaaatcg aagagtcgct
     6001 gaacggcgtg cgttatcaac atcatattca gctcatcaca ctatggtttg atgatgcgct
     6061 cagtgccatg cgttcgctga aaggcatcgg tgctacgcat cttcatgaag ggcgcgaccc
     6121 gcgaatatta acgcgttcgc agttgcagcg attgcaactg gcctggccgc aacaacaggg
     6181 gcgatatcct ctgacgtatc atcttttttt gggagtgatt gctcgtgagt aaacgttatt
     6241 ttgtcaccgg aacggatacc gaagtgggga aaaccgtcgc cagttgtgca cttttacaag
     6301 ccgcgaaggc agtaggttat cggacggcag gttataaacc ggtcgcctct ggcagcgaaa
     6361 agaccccgga aggcttacgt aatagtgacg cgctggcgtt acagcacaac agcagcctgc
     6421 agctggatta cgcaacggta aatccttaca ccttcgcaga atccacctcg ccgcacatca
     6481 tcagcgccca agagggcagg ccgatagaat cattggtaat gagcgccgga ttacgcgcgc
     6541 ttgaacaaca ggctgactgg gtgttagtgg aaggtgctgg cggctggttt acgccgcttt
     6601 ctgacacttt cacttttgca gattgggtaa cacaggaaca actgccggtg atactggtag
     6661 ttggtgtgaa actcggctgt attaatcacg cgatgttgac tgcgcaggca atacaacacg
     6721 ccggactgac tctggcgggt tgggtggcga acgatgttac gcctccggga aaacgtcacg
     6781 ctgaatatat gaccacgctc acccgcatga ttcccgcgcc gctgctggga gagatcccct
     6841 ggcttgcaga aaatccagaa aatgcggcaa ccggaaagta cataaacctt gccttgttgt
     6901 agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga ctgcaattta
     6961 accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat gcggcgaatg
     7021 tgggaattgc ccaggcggcg ggggataggg gctggagaca gttatccact attcctgtgg
     7081 ataaccatgt gtattagagt tagaaaacac gaggcaagcg agagaatacg cggcttgcac
     7141 gcgaattggc gttaaagacg gctcaaagaa atatctttta ttttttaact ggttagataa
     7201 atgcaatggc agtcactgaa caggcatctc ttgccataaa actgtcatca ctcatcttga
     7261 caaatgttaa aaaagccgtt gctttgggga taacccggta aggctggagt tttatctcgc
     7321 cacagagtaa attttgctca tgattgacag cggagtttac gctgtatcag aaatattatg
     7381 gtgatgaact gtttttttat ccagtataat ttgttggcat aattaagtac gacgagtaaa
     7441 attacatacc tgcccgccca actccttcag gtagcgactc atgagtaaac cgttcaaact
     7501 gaattccgct tttaaacctt ctggcgatca gccagaggcg attcgacgtc tcgaagaggg
     7561 gctggaagat ggcctggcgc accagacgtt acttggcgtg actggctccg ggaaaacctt
     7621 caccattgcc aatgtcattg ctgaccttca gcgcccaacc atggtacttg cgcccaacaa
     7681 aacgctggcg gcccagctgt atggcgaaat gaaagagttc ttcccggaaa acgcggtgga
     7741 gtatttcgtc tcctactacg actactatca gccggaagcc tatgtaccga gttccgacac
     7801 cttcattgag aaagatgcct cggttaacga acatattgag cagatgcgtt tgtccgccac
     7861 caaagcgatg ctggagcggc gtgatgtgat tgtagtggcg tctgtttccg cgatttatgg
     7921 tctgggcgat cctgatttat atctcaagat gatgctccat ctcacggtcg gtatgattat
     7981 cgatcagcgc gcgattctgc gccgactggc ggagctgcaa tacgctcgta atgatcaagc
     8041 attccagcgt ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc cggcagaatc
     8101 ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat tgtcgttatt
     8161 tgacccgctg accgggcaga ttgtttccac tattccacgt tttaccatct acccgaaaac
     8221 gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg gaggagatca aagaagaact
     8281 ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc agcggctgac
     8341 ccagcgtacc cagtttgatc tggagatgat ggacgagctg ggctactgtt cggggattga
     8401 aaactactcg cgcttcctct ccggtcgtgg accgggtgag ccaccgccga cgctgtttga
     8461 ttacctgcct gccgatgggc tgctggtcgt cgatgaatct cacgtcacca ttccacaaat
     8521 tggcggcatg tatcgcggtg accgggcgcg taaagagaca ctggtggagt acggcttccg
     8581 cctgccatca gcgctggata accgtccgct taagtttgaa gagttcgaag cattagcgcc
     8641 gcaaaccatc tatgtttcgg cgacgccggg taattacgag ctggaaaaat ccggcggcga
     8701 tgtggtggat caggtggtgc gtccaaccgg attgcttgac ccgattatcg aagtgcggcc
     8761 ggtggcgaca caggttgatg atcttctttc ggagattcgt cagcgagcgg caattaacga
     8821 acgcgtgctg gtcaccacac tgaccaagcg aatggcggaa gatcttaccg aatatctcga
     8881 agaacacggt gagcgcgtgc gttatcttca ctcagatatc gacaccgtcg agcgtatgga
     8941 gattatccgc gacttgcgtc tgggtgagtt cgacgtattg gtagggatca acttactgcg
     9001 tgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg acaaagaagg
     9061 cttcctgcgt tccgaacgtt cgttgatcca gaccattggt cgtgcggcac gtaacgttaa
     9121 cggtaaagcg attctctacg gcgataggag cactccatca atggcgaaag cgattggcga
     9181 aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag gaacacggca ttacgccgca
     9241 aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg cagaacattg ccaaaaccaa
     9301 agcgaagggc agaggaaaat cgcgcccgat tgttgagccg gataatgtgc cgatggatat
     9361 gttgccgaaa gcgttgcagc agaaaatcca tgaactggaa gggctgatga tgcaacacgc
     9421 gcagaatctg gagttcgaag aagcggcaca aattcgtgac cagttgcatc agttgcgtga
     9481 gttgtttatt gccgcatcgt aacaggatag cgaagaagac tgatgacaaa cggaaaacag
     9541 tctgatgcgc tacgcttatc aggcctacat tttctccgca atatattgaa tttgcgcggt
     9601 ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgcgca ctttgtcttc
     9661 aataggaagc cggaattttc cttcggattt ccgttaacct aaagcctgta acgccttttc
     9721 cagcgcgtta tgtaacaact ggcggtcatg acgatacgga atatcgctgg cctccagtac
     9781 ctcctggatc acaatccgct ctttcaccgc cgagacatcc acttttggcc cgacgatgac
     9841 cgcatcaatg acctttttac caacatactg ctccataatt gccagcttgc tttccagctt
     9901 caaattagcc gcaggtaaac tcaactcacg ccccagattg ccgatataaa ccatcggcgc
     9961 tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc agaattggca tcaggctggt
    10021 ataaaaactg ccaggcccaa taatgatgag atccgcttca ttgatagcgt gaaccgcctc
    10081 acgcgttgcg ggtacattag gcgttaacaa taactcttga atcggcgtag ttaactggtc
    10141 gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca gatcaacagg
    10201 atgctctgac attggaatca aatgcgtatc cactttcagc agattacgaa ttaaattgat
    10261 ggcttccaga ggccgcacgc taaggtgatc cagcgccttt aacatcaagt ttccgagatt
    10321 atgaccggaa agttcgccat tgccgccaaa acggtattca aacatcgcgg aggcgacgtt
    10381 cggttccgtt atcagctggt tgaggcagtt gcgcatatcg ccccaagcaa tgccgccttc
    10441 tgaacggcga atacgccccg tcgagccacc attatccgtg gtggtgacga tacccgttaa
    10501 acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc ctccgccgag
    10561 agcaacgaca cgatcaagat cagccagcgt acgattgcgc atatagtttc ctggagtcag
    10621 attatccgcg ctacagtagc gcaaattgcg gggaaacagc aattaacctg ccaatattga
    10681 ggatacaaac tattttcttc tatctctaaa ggacgatgca cgctatgcct ccctgatgat
    10741 gtatatcaaa gtaaaaccgc cattttccct tattctgtag cgaaatagca cgatcatgac
    10801 gctatataca tgattacata gcgaaagtgt ggatggtaaa aatctcattt acacgctagt
    10861 atcggcttaa ccactaaaca ctctagcctc tgcacctggg tcaactgata cggtgctttg
    10921 gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg aagaaatgac ttcgcctccc
    10981 gtatctggaa aggtgtacat ggcttcacaa ctgactgatg tatttgcgcg taagttttac
    11041 tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt gcacctactg cctgccggat
    11101 ggctacaaac cgagcggcgt caccaataaa ggctttctta ccgtcgatga aattcgccgg
    11161 gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg aggagagccg
    11221 tctttacgcc gtgactttac cgatatcatc gccgctgtgc gggaaaacga cgctatccgc
    11281 cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa ctggcgcgat
    11341 gcgggactta ctggcattaa cgtcagtgtc gacagtttgg acgcccgcca gtttcacgct
    11401 attaccgggc aggataaatt caaccaggtc atggcaggga ttgatgctgc atttgaggcc
    11461 ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg atgttaatca tcaccagctc
    11521 gacacctttc tgaactggat ccagcatcgc cctatccagc tgcgtttcat cgaactgatg
    11581 gaaacgggcg agggcagcga gctcttccgt aagcatcaca tctctggtca ggttctgcgt
    11641 gacgagctac tgcgtcgcgg ctggatccac caattacgtc aacgcagcga cggtcccgcg
    11701 caagtctttt gccatccaga ttacgccgga gagattggcc ttatcatgcc gtatgaaaaa
    11761 gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact ccatctctgc
    11821 ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg aaggcgatac ccagcaacag
    11881 gcgctggaag cgcgtatttc agcggcgctg cgggagaaga aacagaccca tttcctgcat
    11941 caaaacaaca ccggtattac gcaaaactta tcgtacattg gcggctaaaa cgtcaaaagg
    12001 agagatcaga tgagtcaggt aagcactgaa tttatcccga cccgtattgc tattcttacg
    12061 gtttctaatc gtcgcggtga agaagacgat acctccggtc actatctgcg cgattcggcg
    12121 caagaagcgg gccatcacgt tgtcgatgaa gccattgtga aagaaaaccg ctacgctatt
    12181 cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac aagtggtatt gattacgggt
    12241 ggtactggcc tgacggaagg tgatcaggct cccgaagcgc tgctgccgtt gttcgaccgt
    12301 gaggttgaag gttttggtga agtgttccgt atgttgtcgt ttgaagagat tggcacttcc
    12361 acgttgcaat ctcgtgcggt agcgggcgtc gctaacaaaa cgctgatttt cgccatgccg
    12421 ggttcgacca aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca gctggatgcc
    12481 cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg caactgaccc
    12541 atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc cgccaaagcg gaaaccgtgc
    12601 gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg atgattattg
    12661 atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg tattgccggt attcaggcgg
    12721 caaaacgcac ttgggatctg atcccgctgt gtcacccgct gatgctcagc aaagttgaag
    12781 tcaatttaca ggccgagccg gagcacaatc gggtgcgtat agaaacctta tgccgcctga
    12841 ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg gcgctgacca
    12901 tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta cgtttgctgg
    12961 cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc ggatgattaa agttcttttt
    13021 ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc ggattaccca
    13081 accgttgaag cgttacgcca gcacctggct gcgcagagcg atcgctgggc gctggcgctg
    13141 gaagatggca aattgctggc tgccgtcaac cagacgctgg tgagttttga ccatccgctg
    13201 actgacggcg acgaagtagc tttcttcccg ccggtaaccg gaggttaaga tggcagaaac
    13261 caaaattgtt gtcggcccgc agccgttcag cgtaggagaa gagtacccgt ggctggcgga
    13321 gcgtgacgaa gacggtgcgg tagtcacctt caccggaaag gtgcgcaacc ataacctggg
    13381 cgacagcgtc aaagcattaa ccctcgaaca ctatccgggg atgactgaaa aagcactggc
    13441 agaaattgtt gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg tgattcaccg
    13501 catcggggaa ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca gtgcgcatcg
    13561 cagcagtgcg tttgaagccg ggcagtttat tatggattat ctcaaaaccc gcgcaccgtt
    13621 ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt gaagcccggg agagcgatct
    13681 gcaggcggca aaacgctggt agttttttgt tagccggata aggcaccgct gcgtccggca
    13741 atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc acagcctcat cattttgcag
    13801 tatccttaag atattcctta tatcttcagg agatcgtcat ggacagattc ccacgttctg
    13861 attcaatcgt acaaccctgg gctggcttgc aaacttatat ggcgcaagtc tatggctgga
    13921 tgaccgttgg cttgttgctg accgcatttg ttgcctggta tgcggctaat tccgcggccg
    13981 tgatggagct gttgttcact aaccgtgtct ttttaatcgg tctgatcatc gcgcaattag
    14041 cattggttat tgtgttatca gcgatgattc aaaagctgag cgcaggtgta acgacgatgc
    14101 tctttatgct ttattcggcg ctgacgggtc ttacgctttc cagtatattc attgtctata
    14161 ccgctgcttc tatcgccagt actttcgtcg ttactgccgg gatgttcggc gcaatgagcc
    14221 tgtacggtta caccacgaag cgcgatttaa gtggctttgg caatatgctg tttatggcgt
    14281 taatcggcat tgtgctggca tcgctggtca acttctggct gaaaagcgaa gcattgatgt
    14341 gggcagttac ctacatcggc gtgattgtct ttgtcggatt gacggcgtat gacactcaga
    14401 aattgaaaaa tatgggtgag cagattgata cccgcgacac gtcgaacctg cgcaaatatt
    14461 ccattcttgg tgcgttaacc ttgtatctgg acttcatcaa cctgttcctg atgttgttac
    14521 ggatcttcgg caaccgccgt taattctttt tcaccatcac tatcccggcc atgtgtcggg
    14581 atttttttct tactaaaaac gacacttaac aagtaccagg tataaaatat gccttatgag
    14641 cgagcggaat cgttttccgt atccataaat acaaaagtat tttgtatgcg tggttttaaa
    14701 tttattgatg gtgaatatta atgttttttc taaggacaac taaaagaatg gaatcatact
    14761 cgcaaaacag caataaatta gattttcagc acgaggccag gatattaaac ggtatatggc
    14821 tcattaccgc tttaggtttg gtggcaaccg caggactagc ctggggagct aagtatctcg
    14881 aaattacggc aaccaaatat gattcaccac caatgtatgt cgccataggg ttattattac
    14941 tttgtatgta tggcttaagt aaggatatca acaagataaa tgccgccatc gcgggcgtaa
    15001 tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt cctgtatatg
    15061 ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg ctggccggtt
    15121 tattatttaa tgttgatcct ggttctcacc gttttatcat tatgatgacg ttgacagggt
    15181 tggccctggt aatcatcgtg aatgcggcat taatgagtga acggcccatt tgggtaataa
    15241 gttgcttaat gattgtgtta tggccaggca ttatctcgca tggacgaaat aagctccttg
    15301 aactggcagg aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc gcttttacag
    15361 gtgcattaac actctattac tattttatcg gcttctttgg gatacttgcc gcgatagcta
    15421 taacgcttgt ctggcaaagg catacgcgtt tttttcatta gtcgtaaatg cagatactaa
    15481 agaacttaac taaacttcac atcgccgctt catttttcgc ccgtagcttc ttcgcttggc
    15541 tttccagtag caggtaacag atcagcgcca gcagcagcgg gataaagtaa tacaggacgc
    15601 gataagcgag cagggcggcg ataatgctcc ctttcgaggt atgctcccca gccagtagtg
    15661 cgataaacac cgcttccagc acaccgatcc ccgccggaat atgcacgatg acgccagcaa
    15721 tactactaac cagtaacacg cccagtacaa agaaatagtt cacgctttga ccaagtaaca
    15781 gccagataat cgcccccatt accatccagt taacgctgga aatcagcatc tgagcgaggg
    15841 cgaatttcca tgaaggcagc accagctttt gccctttgat ggtcatatgg cggtgcttcg
    15901 cgaaagcgca aaaccacaaa taaaccgcga taatcatcag taagccaatg ccgagaatac
    15961 gcagcgtagt ttgatcgaca taccagtaat ccggcaactc caccacgcct gcggtaaaga
    16021 taatccctgc cagtaaaata tagcccagcc agttggtggt aatactgagc gaggaaatcc
    16081 gcgtaatagt gctgcccggt aaccctagtc gggagtacaa acgatagcgc ataccaatgc
    16141 cgccgaccca ggtactgagc gtcaggttga aggcgtagca gataaacgac accagcatca
    16201 cctggcgctt cgccagtttg tgaccgcagt aaaagcgggc gagcaggtca tagcagccgt
    16261 aaatcagata gctgacgacc accagcccga ccgcactaag cagcgcaacg cgattgtagt
    16321 cgcggatgac cttccagacc tcttcccagt ccactttttt ggcgtagacc accagcaaca
    16381 caatcaccgc gataaaaaac agccaggtga ggatcttctt tgctaagcgc cagcgcgggt
    16441 gtgatttact catcaggggt ttacccccgt gttttcagtt tctacccgat cctgcgtttc
    16501 cattgtcggt tgtgcggggg gatcaacctg cgtcagacgt ggtgtgtgtg ccggaagcca
    16561 gccaaccagc gccgggaagt ggcgtaaaaa gtggaacgcc agcacgcttt tggtcaggtt
    16621 ccaccaggtg cgtttgggca gcatggtttc atccacctgc tgacaatctg cggcaataat
    16681 gccgttcaga ttatcgcgca gcgtctggtt aaaatgacga tcgtggatga tgacatttgc
    16741 ttcgagattc agtgacaaac tgagcggatc gagattactg gaccctactg tcgcccagtg
    16801 atcgtccatc aatgccactt tgccgtggag cgggcggcgg cggtactcaa aaacctgaac
    16861 gccgccttta accagatagt tatacagcaa gcgcgcaccg actctgacaa tcggcatatc
    16921 cggttcgccc tgaatgatca gtttgatccg caccccgcgc cgtgccgctt tacgcaaggc
    16981 gtgtaaaaat cgatagccgg ggaagaagta ggcgttggcg ataatcactt cccgctgcgc
    17041 ctgagtgagc attttcagat aatggcgttc aatatcatcg cgatgttctt cgttatcgcg
    17101 ccagaccagc aatacctgcg cttctcccgg ctggcggttc tcttccgctt tgtgatgacg
    17161 tcgccaccag cgtcgtgccg cgctctgtcc aggcaggttt tccagctcaa actggagaat
    17221 atcttcgaca atcggccctt caaggcgtac cgcgtaatcc tgtttagcct ctggaccgta
    17281 gctggacata tgctcggcgg agtaattcag cccgccaata aaggcgatac gcgcgtcgat
    17341 caccacaatt ttgcgatgca tccggcgaaa cacattggtg cgcataccaa aaaggcgagg
    17401 gcggggatcg tagtagcgga acactacgcc agctgccgtc agttcattga caaactcatc
    17461 gctgagatcc ggcgaaccgt agccatccag caagacttcc gctttaaccc cgcgttgcgc
    17521 tgctgccagt agtgccgcat gcagctgttt gccgacgtta tcctcaaacc agataaacgt
    17581 ttcaagaatg atgcgttctt gtgcctcgcc aatcgcctta aacaccgcgg gataatattg
    17641 ctcgccgttt tccagcaact ggatcttatt gccttcgcgc cagctacatt tcataaatga
    17701 atctccgcac ttaaaggggc atgatcagaa aggtgtcgcc atgtccgcag cggcaacgcg
    17761 gttggcgcgc tggcgctggc atttttgacg tagatcctgt ccagtcgtag tagaggaaat
    17821 tgcaccggaa acgtgcgcgc cgggcgtccg tgggcgcggg taaaaatctc atccagtccg
    17881 gcctgcactt ttaacggatg attagctttt tgccgccagt cattgaaatc acccgccacc
    17941 aataccggtt cgccgtccgg tagctcattc acccattcgg cgagcatcgc aagctgcgcc
    18001 tgacggtgcg cctcacgcag gcccagatgt acgcacatca catgaatcgc ttttccggtc
    18061 atcggcggca caatgcggca gtagagcacg ccgcgctttt ccgcaccatc gaccgaaaca
    18121 tcgcgattct cataatgttc aatgggataa cgcgacagta cggcgttgcc gtgatgccct
    18181 tccgggtata cggcattgcg accgtaggca aaatcgctcc acatagtgtc ggcgagaaac
    18241 tcgtagtgcg aggtatcggg ccagttttcc acatgcagcg gatgaacttc gtgcgcgccc
    18301 atcacttcct gcaggcaaac aatatcggcg ctgacggtac gcacggcatc gcgaagttcc
    18361 ggcaaaatga agcgtcggtt aaacgcggta aagcctttgt gaatgttgat ggtgagcacc
    18421 ttgaacgaaa attgttgtgt ttgatcgggc ataattttcc tgtctttgcc tcttatctca
    18481 ttgaaatagt gtagtcggcg tcacaaaaag gtgcggtctt acggaatttt ccgtaaagtt
    18541 cggtactctg agtaagtaga gataaattct tcaggagaga agccatgaag tggcaacaac
    18601 gtgttcgtgt cgcaacgggt ctaagttgct ggcagattat gttgcattta ctggtagtgg
    18661 cgctgctggt ggtgggctgg atgagtaaga ctctggttca cgtcggcgtg ggattatgcg
    18721 cactgtattg tgtcacggta gtgatgatgc tggtgtttca gcgccacccc gagcaacgct
    18781 ggcgtgaggt ggcagacgtg ctggaagagc tgaccacgac ctggtatttt ggcgcagcgc
    18841 tgattgtgct gtggctgttg tcccgcgtac tggaaaacaa ctttttgctg gcaattgcag
    18901 ggctggcgat ccttgctggc ccggcggtag tgtctttgct ggcgaaagat aagaagttac
    18961 atcaccttac gtctaaacat cgcgtacgcc gctgaccctg tcgtggccgt tatcaccagt
    19021 agcggccaca aactattcca cacaatatcc agactcgcat ccttcaaata aatctgcttg
    19081 gtaatgtccg taaagtggcg aatagggtta atccacgtca gattttgcag ccacactggc
    19141 atgttttcca ccggcgaaac gtaaccggaa aggagaatgg cgggcatcat aaaaacaaac
    19201 acgccgataa acgcctgctg ttgtgtcgag cagagtgatg aaatcaacag accgaatccc
    19261 accagcgata aaccgtaaat caccatcgta aagtagaaca gcgctagcga tccggcgaag
    19321 gggatttgat acgcccagat accaatcgcc agcacaatgg tggcctggaa ggtggcgaca
    19381 attaacgccg gtacggcttt gccgatgaag atctgccagg tggtgagcgg cgaaaccagt
    19441 agctgatcga gcgtaccttg ttcacgttcg cgggcgacgg aaagtgaagt gacgatcatt
    19501 acgccgatag tggtgatcat ggcgatcagc gacggcacca caaaccattt gtagtcgaga
    19561 ttcgggttat accagttgcg taccaccagc tcgctgttgt tgggtttcgg ttttccttcc
    19621 agcagctcct gctgataatt tttgacgatc tgttgcaggt agttggcggc aatttgcgca
    19681 ctgttggagt tacgcccgtc gaggatcaac tgcaacggcg cggtctggaa ggtatccagt
    19741 ttgcgcgaga agtcagccgg gaaaagcacc agtagtaacg ccttttgtgt gtcgatggtc
    19801 gggcggatct cctgtgggct tttcagcagc agcacatgtg taaaggcgct ggcgcgggca
    19861 aaacgttgag taagttccac cgaatgctcg ccgttatctt catcgtagat ggcgatggtg
    19921 gcgttagtca cttccagcgt ggcggcgaac gggaacagga tcacctgaat tagcacgggt
    19981 aaaatcagaa tcgcgcgggt ttgtggttcg cgcagcaacg actgcaactc tttgcggatt
    20041 aacgtccata agcgatgaaa catgctcttc tccctaatcc agccgacgtt tggttttcag
    20101 ccacgtcagg ccgataaaca tcaccgccga agcgatcaaa aacagcacgt ttaccaccag
    20161 caccactgga atattcccgg cgaggaacag gctttgcagg gtgctgacga aataacgagc
    20221 gggaataatg tacgtcaccg cgcggatcac cgcgggcata ctgtcgatct gaaaaataaa
    20281 gccggaaagc ataatcgacg gcagaaaagc ggcgttcagg gcgacctgag cggcattgaa
    20341 ctggttgcgg gtaatcgtgg aaatcagcag ccccattccc agggtactga gtaaaaacag
    20401 gctggagata aaaaacagaa tcagcagcga accgcgatac ggcacgccga gaataaacac
    20461 tgacaccagc atacacagca acatcgccag catcccgaga aagtaataag ggatcagctt
    20521 acacagcagc agttccgtgc gggtaatctc cgtagagagc agagcctcca tggtgccgcg
    20581 ttcccattct cgcgccacca ccagcgaggt gagaatcgcg ccgatgaccg tcatgataat
    20641 ggtcaccgca cccgggataa taaagtgctg gcttatcgcc gccgggttaa accagtagcg
    20701 ggtttgtaca tcaataagcg gttcaaaagt ctgcccgttg tcctccgctc gctgcatttg
    20761 ccagatctgc cagatccctt cgacataccc ctgcacaaag ttagcggtat tcggctcact
    20821 gccgtcggtg atcacctgaa tcggtgcggt ggcgttggcg cgctccatct gttcggcgaa
    20881 atccacggga ataaccacca gaccgcgaat tttccccgac tgcattttgg cgatcagttc
    20941 ctgacggtta tcgctgatgg tggcgtcgat gtagggcgaa ccggtcatgg tgtgggtgaa
    21001 atccagcgcc gcttcgctac gctgttccag caaaatcccg acccgcagct tgctggagtc
    21061 gaggttaatg ccgtaaccaa aaataaacag cagtagcagt gggatcacta ccgcaatcag
    21121 ccagctactc ggatcgcgaa cgatctgccg cgtctctttg acgcacagcg cccgcacgcg
    21181 atgccaggaa aggatcgggt tactcattgc tatgctcctt atcccagtcg tggatcaact
    21241 gaataaaggc ttgctccatg gtgggatcgg gttgctcatc gttagctgac tgtgctttca
    21301 aatcgtccgg cgtaccgctg gcgattaatt tcccacggta caccaggccg atgcggtcgc
    21361 aatattccgc ttcatccata aagtgggtgg tgaccatcac cgtgacgcct ttctctacca
    21421 tgctgttgat gtgcagccaa aattcacggc gggtgagggg gtcaacgccg gaagtcggtt
    21481 cgtcgagaaa gagaatatcc ggttcatgca tcagcgaaca ggccagcgcc agccgctgtt
    21541 taaaacctaa tggcagttca tcggtggcgt gtgaggcaat acttttcagg ccaaatgcct
    21601 cgctcatacg ggagattttt tcgttctgcg cccgaccgcg taagccatac acaccagaga
    21661 aaaagcgtaa attctgttcg accgtcaggt tgccgtagag cgaaaatttt tgcgccatat
    21721 agccgagatg ctggcgcgct ttaccggaac tctctttcag atccatcccc agcaccagcg
    21781 cctggccgga agtcggcacc agcaaaccgc acatcatctt aaaggtggtc gatttacccg
    21841 cgccgtttgg ccccagcaaa ccaaaaatct ccccacgttt aacggcaaag ttgacgtgat
    21901 cggtggcggc aaaatcacca aatttcttgg tcagttcttt cgcttcgatc accgtctcgc
    21961 cgggtgtgcc ttctaccgta tgcaatattg cacccagcgg cgattctgag gttccggcac
    22021 cgcccagcaa atcaataaac gcatcttcaa aacgcggcgt agtttcgttg atgttgattt
    22081 ccggcatccc gtcggcatgg cgaatatcgt ctggtgtggc ctcttttttg aggatcagac
    22141 gtaccgattt cccctgaatc atgccatcgc tgacctgcgg cagtttcaag gcgcgttgca
    22201 acagtttgcg gttgccctcg tgtggactgg tcatcagaaa gctgcgtccg gccatggttt
    22261 gtgtcagggc ttttggttct ccctgataca gcaactcgcc ttcgttcatc agtaacacgt
    22321 cacggcactg ctcggcttcg tcgagatacg aggtactcca gaggattaac atcccttcgc
    22381 ccgccagctc atgcaccatc tgccacagtt cgcgccgtga gatagggtca acgccgacgc
    22441 cgggttcatc gagcagcaac actttcggtt cgcccaccag ggtacaggcc aggccgagtt
    22501 tttgtttcat cccaccggag agcttgcccg ccaggcgtcc ggtaaacggc ccaagagacg
    22561 taaactccag caggcgagca aaagtttgct tacgtgcctc gccggtgacg ctgcgcaaat
    22621 ccgcgtacag attgaggttc tccatcaccg tgagatcttc atacagacca aatttctgcg
    22681 gcatatagcc gagcacggcg tgcagcgcgc cgtcgttttt gatcggatca aagccaatca
    22741 ccgtggcact gccgctgtcg ggtttcagta atcccgccaa catccgcatc agtgtggttt
    22801 tacctgcacc gtccggcccc accaaccccg tcacataacc ggcgtgaatg gtacaatcga
    22861 gcggcgcgac ggcgggcttg tccatgcccg gaaagcgttt ttccaggccg ttcagcgtga
    22921 taacggcatc attcatgtcc tgcctcgtca ccgaattgta ccgtcactgg cattccctgg
    22981 cgtaacgcat catcggcgtc ggtcaccaca atacgcaggc gatagacgag gtcggtacgc
    23041 agatccggtg tttcgacggt tttcggggta aattcagcag tcggcgaaac gaaaccaatc
    23101 tgcccgtgat acggcttgtc cgggcgacca tcggtataaa gcagcacttt gcgccccggc
    23161 tgggcctggt caagattccg ttcatcaaca taagcgcgca cccacaccgg acgcgttagt
    23221 gaaacggtaa acaccgtgcc accttcattg agaaccgtgc ctggctctac cgcgcgcgtt
    23281 aacagcgtgc catcagacgg tgctatcaac gttgagtcct gtaaattcag ctccgcctgc
    23341 gccagttgcg cctgcgcctg ttcgaggctg gctttcgcct gagcgatgtc ctgttcacgg
    23401 ttaccggaac ggtactgacg caatttatcc tgtgctgatt tcagcgttgc ctgtgcctgg
    23461 tcgcgcgagg agcgggcatt ttccaggtca tttgccgaaa tggtgcggct tttccacagt
    23521 ccttgctggc ggttatagaa gttctgcgca tagtcatagg cggcttgcgc ctgtttcacc
    23581 gctgcggcgg cctgagcgat ttcttcatcg cgatacccgg caagcatcag atcatactgc
    23641 gcctgtgcca ccgagacgcc cgctttcgcc tgcatcaggg caatctcata cggcttgtga
    23701 tccagttcgc ccagcacctg gcccgctttg atagcatcac cttcgtccac cgccagcgat
    23761 tcaacgcgcc ccccaacacg gaaactaaga ttcaccgtac gaatatccac gttgccatac
    23821 agcgtcaggc cgttatcctg gcggctttga taccaccagt agcctccggc aaccacggcg
    23881 gcaagtacca ctaccgccag tccgatcacg acaggttttt tcatcactac aaactccttt
    23941 gcgataatcc ttgcaaaatc aggtcgatat gacaggtcac cgtctggttg atcagttcgg
    24001 tcttttcttc atcgaacgcg gtccagccgg tacgtaacag aattgtttct ttgccaagac
    24061 ggaacgccag aatctcacca atcaacgcat gggtatgaag gatcatgcgg gtgtcattag
    24121 cgtcgcagcc ggtccaggcg gcaatcaggc gtgtcaggtg gctgtgtagc ggactaatca
    24181 cctgttcgtg caccaggtgg taggctgccg tgggagagag ctgctcacgg gagataaact
    24241 tgctgaggtt gacggtgtca tcctaggtga gcagcttaat catgttcctg caggcgcgaa
    24301 ggatcagttc acggatggca gcccgatcag gctgtggttg ggcgaacaag cgttcggctt
    24361 cctcggcatg cggacggaac tgctcgccaa taaaatcggc aatccactgg gcgcaggcga
    24421 ggtacaaatc ttctttcgaa ccgaagtagt aggtgatggc agcgatattc tgcccggcct
    24481 gggcggctat ctcgcgggtg gtggcgttca ttccatattc accaaactgc gccagtgcgg
    24541 cagcaatcag ctgttttttc gcctgttcac ccttgattgt catggcagga ttattcatcg
    24601 cacagtccat tcttaatcaa atgattgatt aagattatga ctccatatgg gagttgtcca
    24661 gtatggctaa gaattttagc tacgccagtc acagggataa tttatgcgct gcgtcacaaa
    24721 aactgctaca ctccgctccc tcatgacatt gtggtttttg tcattttcct tttcagtatc
    24781 tccctgaaaa ctacaccggt aacggtcggg gcggttcgga gtagttatgt ctttcgattc
    24841 tttgggttta agccctgata tcctgcgcgc cgttgccgag cagggttacc gtgaacccac
    24901 ccctattcag cagcaggcga tccctgctgt gctggaaggc cgcgacctga tggctagcgc
    24961 ccagaccggc accggcaaaa cagcgggctt tacgctgccg ctgttgcaac acctgatcac
    25021 tcgccagccg cacgccaaag ggcgtcgtcc ggtacgtgcg ctcattctta ccccgacccg
    25081 tgaactggcg gcacagattg gcgaaaacgt ccgtgattac agcaaatacc tgaacattcg
    25141 ttcgctggtg gtgtttggcg gtgtcagcat taacccacaa atgatgaagt tgcgcggcgg
    25201 cgttgatgtg ctggtggcaa cgccgggacg tttgctggac ctggaacatc aaaatgcagt
    25261 gaagctggat caggttgaaa tcctcgtcct cgatgaagct gaccgcatgc tcgacatggg
    25321 ctttatccac gatatccgtc gcgtgttaac aaaactgcca gcgaaacgcc agaacctgct
    25381 gttctctgcg accttctctg acgatattaa agccctggcg gaaaaactgt tgcacaaccc
    25441 gctggaaatc gaagtggcac gccgcaatac cgcgtctgat caggtgaccc agcacgttca
    25501 ctttgtcgat aagaaacgca aacgcgaatt gctgtcgcac atgattggta aagggaactg
    25561 gcagcaggtg ctggtgttta cccgtaccaa acacggcgct aaccatctgg ctgagcagct
    25621 caataaagat ggcatccgta gtgtggcgat ccacgggaat aaatcgcaag gtgcgcgtac
    25681 ccgtgcgctg gctgatttta aatcgggtga cattcgcgta ctggtggcga ccgatatcgc
    25741 tgcgcgcggt ctggatatcg aagagttgcc gcacgtcgtc aactatgaac tgccaaacgt
    25801 accggaagat tatgtccacc gtatcgggcg taccggtcgt gcggctgcta ccggtgaagc
    25861 gttgtcgctg gtgtgtgttg atgaacacaa actgctgcgt gatatcgaaa aactgctgaa
    25921 aaaagagatc ccgcgcattg cgattccggg ctatgagccg gacccgtcaa tcaaagccga
    25981 accgatccag aacggtcgcc agcaacgtgg cggcatcagg ctgatgaaca aacgcaaaac
    26041 tgcctgatgc gctacgctta tcaggcctac gtgaactctg caatatattg aatttgcatg
    26101 cttttgtagg ccggataagg cgtttacgcc gcatccggca tttcacaaca agcacttgtc
    26161 agcaatttga gaacacggga aaaaatttat ctgttttacc gcccataagc caccgagacg
    26221 ttacttctct atagccaatt gttcgcgcaa ctccattaaa aggtagccca gtcgattctt
    26281 gcccttacca ttaccaccgt ctccccagta agcatcgttt tccgtatgct caaccagttt
    26341 tgcgggcgcg gttgccagca agagcacacg cagttctgca tgctgttcga atttagcgcg
    26401 aagtgcttta cgcatcacct gttctttaac cgactcccag tttttacgaa gtggcttgga
    26461 acgattacgc cccatgcgag cggcgaccat gggggaagaa acccgacgga tctcttcgcg
    26521 gtatttttca tcaaggaatt tttgtgcctg aaaatagtgt tcggaggtgg gccaggtatt
    26581 cccgtcaacc ttgatgggcc tggcggcaaa gttagaaaaa tcaccgtagt catcgctggt
    26641 gctgtagaaa tttatgatgg tgtcttgcat gacgtgttgg atcctttgtg ctcgaacggg
    26701 cattaaaccg cattatgttg gtggttattg cgagccgctt tccagaaaca gaaaaaccat
    26761 tacccctgaa aaccgaaaaa tgccacaata ttggctgttt atacagtatt tcaggttttc
    26821 tcatggcatt aaccgccgcg cttaaagcgc aaattgccgc ctggtataag gcgcttcagg
    26881 aacagatccc cgactttatt ccccgtgcgc cgcagcggca gatgattgcg gacgtcgcca
    26941 aaacgctggc cggagaagaa gggcggcatc tggcgattga agcccccacc ggcgttggga
    27001 aaacgctctc ctatttgatc cccggcatcg ccattgcccg cgaagagcaa aaaacgctgg
    27061 tggtgagtac cgccaacgtg gcgttgcagg atcagattta cagcaaagat ttaccgctgc
    27121 tgaaaaagat cattcccgat cttaaattca ctgccgcttt cgggcgtggg cgctacgttt
    27181 gtccacgtaa tctgacggcg ctcgccagca ctgaacccac gcaacaggat ctgctggcgt
    27241 ttcttgacga cgaactgacg ccaaataatc aggaagagca aaaacgttgt gcgaagctga
    27301 agggcgatct cgacacttat aaatgggatg gtctgcgcga tcatacggat atcgctatag
    27361 atgatgatct ctggcgtcgt ttgagtaccg acaaagccag ctgcctcaac cgtaactgtt
    27421 actactatcg tgaatgcccg ttttttgtta ctcgtcggga gattcaggaa gcggaagtgg
    27481 tggtggcaaa ccatgcgctg gtgatggcgg cgatggaaag cgaagccgta ctgcctgacc
    27541 cgaaaaatct gctgctggtg ctggacgaag gtcatcacct gccagatgtg gcccgggatg
    27601 cgctggagat gagcgctgaa atcaccgtgc cctggtatcg actacagctg gacttgttca
    27661 cgaaactggt cgctacctgc atggagcagt ttcgcccgaa gaccatccca ccgctggcga
    27721 tccctgaacg tttgaatgcg cattgtgaag agttgtatga gcttatcgcc tcgttaaaca
    27781 acattctcaa tctctacatg cctgccgggc aggaggcaga gcaccgtttt gcgatgggcg
    27841 aactgcctga tgaattgctg gagatctgcc agcggctggc aaaactcacc gagatgctgc
    27901 gtggtctggc ggagttattt cttaacgatt taagtgagaa aaccggcagc cacgatattg
    27961 tgcgtctgca tcggttgatt ttacagatga accgcgcgct ggggatgttt gaggtgcaaa
    28021 gcaaactctg gcggctggcg tctctggcgc aatcttccgg tgcgccggtg accaaatggg
    28081 cgacgcggga agagcgcgaa gggcagctac acctctggtt tcactgcgtg ggaatacgtg
    28141 tcagcgatca gctggaaagg ctgctgtggc gcagtattcc gcacattatt gtcacctccg
    28201 caaccttgcg ttcgctgaat agtttttcgc gtttgcagga gatgagtggt ctgaaagaga
    28261 aagcgggcga ccgttttgtg gcgctggatt ccccctttaa ccactgcgaa cagggcaaaa
    28321 ttgttattcc ccggatgcgc tttgagcctt ccatcgacaa cgaagagcaa cacattgccg
    28381 aaatggcggc ctttttccgt gaacaggtgg agagcaaaaa atatctcggt atgttggtac
    28441 tgtttgccag cggacgggcg atgcagcgct ttctcgacta tgtgacggat ttacgcctga
    28501 tgttgctggt gcagggcgat cagccgcgtt atcgcttagt tgaactgcac cgcaaacgcg
    28561 ttgccaacgg tgagcgtagc gtgctggtgg gcttacagtc atttgccgaa gggcttgatt
    28621 tgaaaggtga tctgctcagc caggtgcata tccacaaaat cgcttttccg cccatcgaca
    28681 gcccggtggt gatcaccgaa ggggaatggc tgaaaagcct caaccgctat ccgtttgagg
    28741 tgcaaagcct gccgagcgcc tcgtttaacc tgattcagca ggttgggcga ctgattcgaa
    28801 gccacggttg ctggggcgaa gtggttatct acgacaaacg cttgctgaca aaaaattatg
    28861 gcaagcgact actggatgca ttaccggtat ttccgataga gcaaccggaa gtccctgaag
    28921 gtatagttaa aaagaaagaa aaaacgaaat ccccacgccg tcggcggcgt taatgatgtg
    28981 agtcaggtaa ggagtcgtaa atggactatc gcaaaatcat taaagagatc gggcgcggga
    29041 aaaaccacgc gcgcgattta gaccgggata ctgcgcgcgg tctgtatgct catatgctca
    29101 atggtgaagt ccctgacctt gagttgggcg gcgtattgat tgcgctgcgt atcaaagggg
    29161 aaggggaagc agagatgctc ggcttttacg aagccatgca aaatcacacc atcaagctga
    29221 cgccgcctgc aggcaagccg atgccgattg tcatccccag ttacaacggc gcgcgtaaac
    29281 aggctaacct gacgccatta ttggcgattc tgctgcataa actcggtttt cctgtggtgg
    29341 ttcacggagt tagcgaagat ccaactcgcg tgctgacaga aaccattttc gaactgatgg
    29401 gtattacacc aacgttgcac ggcgggcagg cgcaggcgaa gctcgacgaa catcaaccgg
    29461 tgtttatgcc tgtcggcgcg ttttgcccgc cgctggaaaa acaactggcg atgcgctggc
    29521 ggatgggcgt gcgtaacagt gcacataccc tggcgaaact ggcgacgcca tttgccgaag
    29581 gtgaggcact gtgtctttcc agcgtttcgc atccggaata cattggacgc gtcgcgaagt
    29641 tctttagcga tatcggcggg tgggcgttac taatgcatgg tactgaaggc gaagtgtatg
    29701 ctaatccgca gcgctgcccg cagatcaatc tcattgaccg tgaagggatg cgggtgctgt
    29761 atgaaaaaca ggaccctgct ggtagcgagt tactgccaca agcaaaagat ccggaaacca
    29821 cggcgcagtg gactgagcgt tgcctggctg gcagcgaagc gattcccgaa tcgctgaaaa
    29881 tccagatggc ttgctgcctg gtggctacgg gtgaagcggc aactatcagc gatggcctgg
    29941 cgcgcgttaa tcaggcattt taatttttat ctcagccgga tgagccacgt catccggctt
    30001 ttaccccacc gcaatatgaa attcctgcat ctttattgac cttcccgcgc ccggcgtgca
    30061 gcataaaaaa acaacaaaca cataacataa acaggagtta accatggaaa gtggtcatcg
    30121 ctttgatgct cagacgctgc acagttttat tcaggctgta tttcgtcaga tgggtagcga
    30181 ggaacaagaa gcgaaattag ttgccgatca tttaatcgcg gcaaacctgg cagggcatga
    30241 ttctcatggt attggcatga tcccaagcta tgtgcgttcc tggagtcagg ggcatctgca
    30301 aattaaccat catgccaaaa tcgttaaaga ggcgggggcg gcagtcacgc tcgatggcga
    30361 tcgcgcattc ggtcaggtcg cggcacacga agcgatggcg ctggggattg agaaagcgca
    30421 tcagcacggt attgctgccg tggcgctaca taactcgcat catatcggcc gtatcggtta
    30481 ctgggcggag cagtgttcag cggcggggtt tgtctctatc cactttgtta gcgtggtcgg
    30541 tattccaatg gtcgcgccgt tccacggtcg cgacagccgc tttggcacca atcccttctg
    30601 tgtggttttc cctcgtaaag ataatttccc gctgttgctt gattacgcca ccagcgccat
    30661 tgcatttggc aaaacccgcg tcgcctggca taaaggcgtc cccgtgccgc caggttgcct
    30721 gattgacgtt aacggcgtgc cgacgaccaa tccggcggta atgcaggagt cgccgttggg
    30781 gtcgctgttg acctttgccg aacataaagg ctacgccctt gcagcgatgt gtgaaattct
    30841 tggcggggcg ctttctggcg gtaaaacgac gcatcaggaa acgttgcaaa ccagccccga
    30901 tgccattctt aactgcatga ccactatcat catcaacccg gaactgttcg gcgcgccgga
    30961 ttgtaacgcg cagaccgaag cctttgccga gtgggtgaaa gcctcgccgc atgatgacga
    31021 taagccgatt ttgctaccgg gcgagtggga agtgaacacg cgtcgcgaac ggcaggagca
    31081 ggggattcca ttggatgcgg gaagctggca ggccatttgt gatgcggcgc ggcagattgg
    31141 tatgtcggaa gagacgttgc aggctttctg tcagcagtta gccagctaaa aaaaagcccg
    31201 tccagtggcg gacgggcaaa caagggtaac ataggatcaa tgagggttag agcatatgcg
    31261 tctgtcggca aacagacagg gaaatacttg tgctggacgt agcgtaaacg cctgatccgt
    31321 ccaacggttc ggattttgta ggcctgataa gacgcggtag cgtcgcatca ggcatctgtt
    31381 gccggatgcg gcataaacgc cttatccgtc ctacgaatta cttataaatt accgcagtac
    31441 cgcttaattt attgttgttg gtggcggaag tgatgctgta tccactggca ccggctgccg
    31501 ccgctttctc agccagtttc gcttccaggg catcgagggg ggatgcgcca tcggcagaaa
    31561 ccacgccgat tttattcatg ttctgtgcct gggatgccgt taccggttcc gccgcgaaca
    31621 cgccaaatga cagagttgaa agagccatag cagcaacaac agtattgata gttttcataa
    31681 ttaatctctc gcaggtgatt tttgtataag tgacgttgtt tcgtcgatgt gatgagtatc
    31741 acgttttttt acgagagata aaatcgaaga gaattgaccg ccttgttcaa ataaattgat
    31801 tgatatctaa catactgata aaccatttat ttgttgtact tatggacttg ttttatgccg
    31861 cgtggcagtc acagcagcgt taaaggcacg gaatgccacg caaaatacaa attatactcg
    31921 ctatgcatct cgccagttgt agttaacgta actgtctgtc tttcgaacct ctgcgattat
    31981 atcctgtata cgctggtttt tgtaaatctt tctcctgctg acaatgaata cataagcgca
    32041 cgccaggaat ggcttcccga cgggcttgcg ggatgggggc accgcactct tcacattcat
    32101 acaggctttc gccacgcgga atttcacccc gagcgcgggc aatcgcatct tcaattgtac
    32161 tgttgatctg ttcgttgacg gcgtcatcgt tagcccaagc ggatgccata tcgtcctccc
    32221 catatcaatg cttgtacagt taagtgtagc taatataggg acgaacctgg gcagttcaag
    32281 cgtcagatct ccgaccattc ccgcagcaga ttatgataaa gattgagcag cgacaggatc
    32341 tcttcatttt caccatagcg gcttttcagc gactgaatat ttttgtccag ttcaaacagc
    32401 atggcgcgct ttttatcatc gcggatcatc gactggatcc acataaatga tgccactcgt
    32461 acgccgcggg ttacgggtgt cacgcaatgc aggctgctgg agggatacaa caccagatca
    32521 cctgccggga gttttacccg atgttgtcca aaggtgtcat taacgaccag ttcgccgccg
    32581 tcgtagcttt gtggatcgct taaaaacagc gtggcagaaa ggtcagtgcg catccagccg
    32641 ttttgtggat ggctgcgtac tgcgccatcc acatggaaac cataggtttc attgttctga
    32701 tagcgattaa acagcggcgt ggaaagggta cgcggcaagg cagcggcaaa gaacaatgca
    32761 tgttggttaa ccgcgttcag cacctcattt tgcagtgcgg cgtataacgc gctgcgggtg
    32821 tctacttgct gattgttctt aacttgtgca ccttgtgctc cggtggtgac gcgtccatcc
    32881 acccattcgg cttgttccag ttgttcgcga aaatgggcga cgtcctgtgg cgataacacg
    32941 ccgggaatgt ggtacatcat acaagttctc caaaaagtgg ggcctgcgcc ccacatctga
    33001 atcagaaatg catattggct gtgagcaaga aggttcttgg ctcgcccggg tgataacggt
    33061 agccgctctt gttgattgag gcgacgtaat cggtatcaaa caggttgtaa acgtttagct
    33121 ggaagtcgag attgcgatta acccgatacc ccagtttggc atcggcgacc cagtaacctt
    33181 ctgtaaatgc tggcgttccc accgcgccgt ctgaaccttt atgtatactg ccgatatagc
    33241 gtgcgcccgc gccaacagag atatcatcgg ttgcctgata ttggctccat aaggtgaagg
    33301 cgtgctccgg ggtatacggc agcgatgagg aaccatcctg ggcaacatct ttgccgtttt
    33361 tgatggttgc tttttgctgg gtatagccgc caatcacctg ccacgcggga gtgatattcc
    33421 ctgccacgga tatctcatag ccttcgacgc gtttcttacc gtattgcgag taagtcccgt
    33481 catcattttg ctcaacttca ttttcgatat cagtacggaa cagcgcggcg gtgagcaaca
    33541 ggcgtttatc cagaacctgc catttggtgc caatctcgct ggtgttggct ttttgcggtt
    33601 taaaatcggt gcggttggca ctgttaccac tgccagactg cgcaagggcg aagttgttgc
    33661 cgcccggagg ctgctgggaa acggcatagt taatatagac attgccgttt tccgtcaggt
    33721 ggtacagcgc cccggctttc cagttcacca gattgcccga cttggcggtg tcgacggtgg
    33781 tgactggaga accttttgcc acaccagctg ggcaggtgat ggcaccgcgt ccgctgccgc
    33841 cgcaggcggt ggcactgtca tattcagtat gataattatc cagacggatc ccgccgttca
    33901 gctcaaaatc acgggtgatt tgcagcgtat cgaaggcgta aattgcgaag gtatccgtct
    33961 gaccattggc gtttgcgccg ttgcgcgtca ggccgccggg atgaattctg ctgtcaggat
    34021 gataaatatt taccgccggt aacgtcaccg gattaacgcc atagttagtc tgcgtttcac
    34081 gggtaaattc cacgccggta ctgacatcat gaccgataga accggtatag aacgtcgagg
    34141 tcaggttggt ctggttggtg agaattttat tactcacatc tttggtattc gccgtgcgtg
    34201 actaggtcca gctattcaca tcgctggtgg gctgagtaat attcgacgcc ccgcccataa
    34261 tcgccgtcat caggtaatcc tgctttacgc gcgaccaacg ggtagtattg cgaatggtgg
    34321 tgttatcgtt gatgtcgtgc tcaaaacgca tggtggcggt gtcggtggtc gaatcgtcgt
    34381 aatcggaatc cgtgccgtaa aagttatgag tatcaacttt tccggaatga ttcagggccg
    34441 ccgctcccgc agatggggca gaatagcccg gcaaaccgat ggtcggaatg ccgccgtctg
    34501 gcgtgttgtg ctgagtgaca tgcagataat taagatacaa acgatttgct gtaccaaggc
    34561 caaaagcgac agaaggggcg acgccgtaac gctcattttt gactttgtcg cgtccggcat
    34621 cgtgggtttt ttcgcccatc acattcaggc gcaccgcggt agtatcacca atgacctgat
    34681 tgacgtccag cgtgccgcgg cggaaccagg cgctgccaat actggcggag gcgtcaatgc
    34741 cggaatcatt gcgcggctgc ttgctgatca tattgatcga gcctgtcggt gcgctgcgcc
    34801 cgtagtcggt gccggacggc cctttaatca cttcgacctg ctcggtattg aaggtgtcgc
    34861 gcgagacgct gccgatatcg cgaataccat caatataaat actgttagag gtatcggcac
    34921 cacgcatata aatggcgtcg ccagtggtgg agttaccgtt ctcacccgca aaaaacgcac
    34981 ccacgc
//
LOCUS       DAZPAB010000019        34841 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZPAB010000019 DAZPAB010000000
VERSION     DAZPAB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34841
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.019"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(18..93)
                     /locus_tag="ACHUPG_000872"
     tRNA            complement(18..93)
                     /locus_tag="ACHUPG_000872"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:His,seq:gtg)
     gene            complement(152..228)
                     /locus_tag="ACHUPG_000873"
     tRNA            complement(152..228)
                     /locus_tag="ACHUPG_000873"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Arg,seq:ccg)
     gene            complement(331..1716)
                     /locus_tag="ACHUPG_000874"
     CDS             complement(331..1716)
                     /locus_tag="ACHUPG_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0860589.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1907..2647)
                     /gene="wecG"
                     /locus_tag="ACHUPG_000875"
     CDS             complement(1907..2647)
                     /gene="wecG"
                     /locus_tag="ACHUPG_000875"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR0860590.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="ACHUPG_000876"
     CDS             complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="ACHUPG_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR0860591.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="ACHUPG_000877"
                     /gene_synonym="wecF"
     CDS             complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="ACHUPG_000877"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR0860592.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="ACHUPG_000878"
     CDS             complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="ACHUPG_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR0860593.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="ACHUPG_000879"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="ACHUPG_000879"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR0860594.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="ACHUPG_000880"
                     /gene_synonym="wecD"
     CDS             complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="ACHUPG_000880"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR0860595.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="ACHUPG_000881"
     CDS             complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="ACHUPG_000881"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR0860596.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="ACHUPG_000882"
     CDS             complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="ACHUPG_000882"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR0860597.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="ACHUPG_000883"
     CDS             complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="ACHUPG_000883"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR0860598.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="ACHUPG_000884"
     CDS             complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="ACHUPG_000884"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR0860599.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="ACHUPG_000885"
     CDS             complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="ACHUPG_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR0860600.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="ACHUPG_000886"
     CDS             complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="ACHUPG_000886"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR0860601.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14927..16186)
                     /gene="rho"
                     /locus_tag="ACHUPG_000887"
     CDS             complement(14927..16186)
                     /gene="rho"
                     /locus_tag="ACHUPG_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR0860602.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="ACHUPG_000888"
     CDS             complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="ACHUPG_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR0860603.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="ACHUPG_000889"
     CDS             complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="ACHUPG_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR0860604.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16973..18238
                     /gene="rhlB"
                     /locus_tag="ACHUPG_000890"
     CDS             16973..18238
                     /gene="rhlB"
                     /locus_tag="ACHUPG_000890"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR0860605.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18372..19856
                     /gene="gppA"
                     /locus_tag="ACHUPG_000891"
     CDS             18372..19856
                     /gene="gppA"
                     /locus_tag="ACHUPG_000891"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR0860606.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(19903..21924)
                     /gene="rep"
                     /locus_tag="ACHUPG_000892"
     CDS             complement(19903..21924)
                     /gene="rep"
                     /locus_tag="ACHUPG_000892"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR0860607.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22011..22292
                     /gene="ppiC"
                     /locus_tag="ACHUPG_000893"
     CDS             22011..22292
                     /gene="ppiC"
                     /locus_tag="ACHUPG_000893"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR0860608.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(22338..23813)
                     /gene="ilvC"
                     /locus_tag="ACHUPG_000894"
     CDS             complement(22338..23813)
                     /gene="ilvC"
                     /locus_tag="ACHUPG_000894"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR0860609.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            23963..24853
                     /gene="ilvY"
                     /locus_tag="ACHUPG_000895"
     CDS             23963..24853
                     /gene="ilvY"
                     /locus_tag="ACHUPG_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR0860610.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(24850..26394)
                     /gene="ilvA"
                     /locus_tag="ACHUPG_000896"
     CDS             complement(24850..26394)
                     /gene="ilvA"
                     /locus_tag="ACHUPG_000896"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR0860611.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(26397..28247)
                     /gene="ilvD"
                     /locus_tag="ACHUPG_000897"
     CDS             complement(26397..28247)
                     /gene="ilvD"
                     /locus_tag="ACHUPG_000897"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR0860612.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(28312..29241)
                     /gene="ilvE"
                     /locus_tag="ACHUPG_000898"
     CDS             complement(28312..29241)
                     /gene="ilvE"
                     /locus_tag="ACHUPG_000898"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR0860613.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(29261..29524)
                     /gene="ilvM"
                     /locus_tag="ACHUPG_000899"
     CDS             complement(29261..29524)
                     /gene="ilvM"
                     /locus_tag="ACHUPG_000899"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR0860614.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(29521..31167)
                     /gene="ilvG"
                     /locus_tag="ACHUPG_000900"
     CDS             complement(29521..31167)
                     /gene="ilvG"
                     /locus_tag="ACHUPG_000900"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR0860615.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31170..31220)
                     /gene="ilvX"
                     /locus_tag="ACHUPG_000901"
     CDS             complement(31170..31220)
                     /gene="ilvX"
                     /locus_tag="ACHUPG_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR0860616.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(31306..31404)
                     /gene="ilvL"
                     /locus_tag="ACHUPG_000902"
     CDS             complement(31306..31404)
                     /gene="ilvL"
                     /locus_tag="ACHUPG_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR0860617.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            31756..33235
                     /locus_tag="ACHUPG_000903"
                     /pseudo
     CDS             31756..33235
                     /locus_tag="ACHUPG_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(33260..33598)
                     /gene="maoP"
                     /locus_tag="ACHUPG_000904"
     CDS             complement(33260..33598)
                     /gene="maoP"
                     /locus_tag="ACHUPG_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR0860618.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            33717..34556
                     /gene="hdfR"
                     /locus_tag="ACHUPG_000905"
     CDS             33717..34556
                     /gene="hdfR"
                     /locus_tag="ACHUPG_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR0860619.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     DVY"
     gene            complement(34652..34727)
                     /locus_tag="ACHUPG_000906"
     tRNA            complement(34652..34727)
                     /locus_tag="ACHUPG_000906"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34692..34694),aa:Trp,seq:cca)
     gene            complement(34736..34812)
                     /locus_tag="ACHUPG_000907"
     tRNA            complement(34736..34812)
                     /locus_tag="ACHUPG_000907"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34776..34778),aa:Asp,seq:gtc)
BASE COUNT         8499 a         9533 c         8798 g         8011 t
ORIGIN      
        1 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
       61 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
      121 ctaccaccgc agctcaagcg ctgggactaa atggcgcgcc cgacaggatt cgaacctgag
      181 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
      241 cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa ataaattgct
      301 cgtttggtta cggtttgtgc gttttgctgc ttattcctcc agtttatgcg ctttaccgtg
      361 gcgattaagc ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga tgatgccgac
      421 aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa
      481 tgccattgtt acgtagttgg cccacgggaa caggattgag cggaacggat ggctggcaat
      541 cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa accatggcac
      601 catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat
      661 gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca cacccgcaac
      721 cggtacgccg tgacgggaaa cttttgccat tgccgccggt aactgacggt ttttcgccag
      781 tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga gcgcagccgt
      841 cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg caaaagtcag
      901 tacgaacggg ctgccgttgc tgccaatttc attccacggg aagatggtga cgataacgaa
      961 aatcgcgcct acgtagaaaa tcaggatccg ccacagcact ttgcctacgg cactgcgcag
     1021 cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta
     1081 ggacgccacc acaatacaca gagcggtcag gaaccctttc cagccacccg caaagaaacc
     1141 gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa agccaaagaa
     1201 aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa
     1261 ccagaactcg atttcgccgt acaaccgtac cgccgccaga ttcgccaacg ccaccagcgc
     1321 caccgcgatc aatgcgggta tccactgcgc catctccggg gaccaaaact ggacataaac
     1381 gccaatggcg gtgatttctg agatccccac cgccatccac ataaaccagt aagaccaggc
     1441 ggtgagatag ccaaaaaacg ggctcatata acgatgcgca taaacggcga acgaaccggt
     1501 aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaaa cgaacagccc
     1561 ggcgatgata taggccaaca atacggatgg cccggcccat ttcagggtac tggcggcccc
     1621 cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc
     1681 cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg
     1741 ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat
     1801 tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag
     1861 acaggcgacg aagtgaccac tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg
     1921 tgtagtgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg
     1981 aaagcaggcg gtagagccac tccagtccca gcgtttgcca gattttcggt gcgcgtttta
     2041 cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca tctggatgca
     2101 ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga
     2161 tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat
     2221 aaccatcctg actgccaacg atattcacat tccattggtt gcgcagttta gcttcagttt
     2281 gcgccagcac ttcaggttta ccgcccacaa gaaataccgg cgtcccttct ttgcctgcgc
     2341 gcgccatcag ctcttcccag agatcggcac cggcaacgcg ggaaacctgc gcctgcgggt
     2401 actttttacg tactgaacgt acaatgctga tgccatccgc atatttaaat tcggcagcgt
     2461 taattaactc cctgacctcg gcgttatctt caatggtcag cattttttcg gcattaatgg
     2521 caaccagcgt tccctgctta agctgcccgt cagcaaacag ataatcgagg gcatgctgca
     2581 tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt
     2641 tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt tatgaatgag
     2701 tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac
     2761 cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgtg ccagcacgat
     2821 catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat agcgattagt
     2881 ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagtc caaccacaat
     2941 cgccccgagt gggatgaaca acgcgccgcc catcaccacc agtgagccta taagcgtagg
     3001 cgagatcgcc agtccggagt ggttattcag tacttcccag gtaaagtaat tggctgagtt
     3061 cagcaccata ctcgggcgcc ccggccacag ccaggaaggg ataaagacat agaaatcgcg
     3121 gacaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag
     3181 attctcccac ggtgaaaagg tgtcgcgagt gagatagaga aacgtataga acgcttcatc
     3241 gccgctcaca ttcattccat agcgttttaa cgccagccag aacatgccaa caatccccag
     3301 cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag
     3361 gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca tataagtcag
     3421 caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctgt cctggcgcag
     3481 aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc
     3541 ggagacttca ctggaaaaga tctgactgta ggagttaagc cggaacagca aaaagccgtt
     3601 gtgcatgaag aagatgccga cgcttaccag cgcgataccc atcaggatca cccacgtaag
     3661 attggtctcc acgcggttca tggtaaacag cggacggcgc ggtacatcag caacgcgttt
     3721 gcgtaggcgg gttttgtagg tgacatagta aaccgcgtag aagcagcccg cagaaagcaa
     3781 cacctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata ccagcacgct
     3841 ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga agacattgaa
     3901 gttaaagcgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag
     3961 cagccagacc acaaacaggc cactgaattg cagcagactc atgcgacctc cccggcggca
     4021 atcgccaacg cccgctgcca gccttgtaga tagttagggc taaagaaggc aatggtgttt
     4081 ttatccgccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg
     4141 gtagtaaaca gcactggcaa atgctgttcc gtcatatcct gccagaacgg attttccctg
     4201 ttaagcacac aaggaatgcc cgcctgaatc agtaagcaaa gcgtaccaat gccctgctgg
     4261 cgggcaaaaa taaagtaacc aagatcgcac tgacgaagta gcgccagata ggcgtcaaat
     4321 tccagttttt cgctcaaaat ttgcagattt tcttcgctga ataactccag ccccgcctga
     4381 cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac caccactttt
     4441 accgtatcgc caaattgctg atgaacggca cgtaaagcgg cgacatgctc attgctgcgg
     4501 tcgccggagt tccccaccag aatggtcatt tttccttcac gttgccgatc gttcgccatc
     4561 gtattgagag aagggtccat ccgcgtcggg aagtacagca gttctccccg cacctttggg
     4621 tgtgttttgg caaaaaagct caaatcgccg cgggtggcaa atacacagcc gactcgcttt
     4681 tgcgccaggc gacgtagtgg gtaaaaaagc ttatatctca agccactgga aagctcatac
     4741 aggtctgccc cccagatatg ccagaaaaac tggctgggct taatgccacc actcagcaga
     4801 gccagccaca gtgtgggatt gaactgaccg tggaaaaaaa atcgctgctg acggttagct
     4861 tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa
     4921 agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa ctcgcgcgcg
     4981 tgctcgctcg tcgcggccag cgcgtcattg aaaaaccgca aaacggttcg gttatggtga
     5041 gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa
     5101 acacgccaca acaaagagaa aaatagacga tataagttgc catatatgcc tgcgccgcgc
     5161 ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa gtgaactggc
     5221 tgacttccgc cagaatataa aaccgcagtg acgctttggc gatcaccaga taaccaaaga
     5281 cataagcgcc cacttttaac acatcaccca ctaactgcca ggcaaagaga tcgcgcatag
     5341 cggtaaattt attcgacaac agcaaccaga tagcaaaatc acgcagcaac cagacggtaa
     5401 aactcgctgc cgccactgcc ggtaagacga atttcagcga tttaaccact tcccgggtga
     5461 tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg aacgatgccg
     5521 taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc cccacttcat
     5581 cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca ggcaaggtca
     5641 ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga ccgttatccc
     5701 agctgggttt cagatagctt aacgggatga caccacgttt aatcaacatg atggcggcag
     5761 gaattaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca taaccgccca
     5821 aacggtaact gacgtagtac gcgagaacgc caatcaagct gccgacaatc agcgataacg
     5881 cattacctgc ggcatcgcga aagcctttca tcagcgccag taacaggttg ccccaggcga
     5941 tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcggta ttaccaaaca
     6001 atccctggct gattggcgca gctgccagca caaaaaccag cgccatcagc gtagagaaac
     6061 caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgcgga ttatcatggt
     6121 actgggcaac gtatttggtt acaccgttaa agatgccagc cccggcaagc acgccgagca
     6181 cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca aatgacaccg
     6241 ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg gccgccgtcc
     6301 acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaagt cgcaattacc
     6361 gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag gcgctcgctc
     6421 tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agtgttcccc cgcagggcaa
     6481 ccgtgcagcg gaatgtaatg aaacaccgcc atgatttccg cttctttcag aaagttaatc
     6541 aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc gttctgcacg
     6601 cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg cgctaacgta
     6661 tcgtagtagt tttgccacag cgccagacgt tgctggttga tacgatccgc tgcttccagt
     6721 tgcgcccaca ggtacgcagc ttgcagatcg gacatcaaat agctggagcc aatatcgcgc
     6781 caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc cttttcacgg
     6841 atgatctcgg ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc gccttcacca
     6901 cccgccgtgt agtttttggt ttcatggaag ctaaagcagc caatatgacc aatggttccc
     6961 agtgcacgcc ctttgtaagt ggacatcacg ccctgagcgg catcttccac cacaaacaaa
     7021 ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacacc cgcgtaatgg
     7081 accggcacga taacgcgcgt tttgtcggtg atcgctgctt caatcaacgt ttcgtcgatg
     7141 ttcatggtgt ccgggcgaac atccacaaag acgattttcg cgccacgcag cacaaaggca
     7201 ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg ctggatatcg
     7261 agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg
     7321 ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc atcgccacac
     7381 agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac caccggcggt
     7441 gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt ctacattcgc
     7501 accactttgt atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg ccacccgcaa
     7561 agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct cagcacctgc
     7621 accgcgtcca gccagcaggc caattcgcgc atctgtcgca ttgagttccc gtaaagagac
     7681 atagccgcga atatcgccgg acgccgcacg tagaatcaga cattgatgat caaaggtgcc
     7741 gcgcacggca ttttcaatcc actgtgcata aaagcgaccg ctggcgtcag gcgcatacca
     7801 cggcgcacga aaacggcttt gcgcaaatgc ggcgctagct aactgacgta atgcgggaat
     7861 atcggtctct tgtgccacga cagcaccgct atcactgaca ttgttcacgg gtagcgccaa
     7921 atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca gttcccccgt
     7981 atttgatgcc gcaattttgg cctgcacccg tgaccatggc gctaaggcgt ctgacgtcag
     8041 gagcggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga aggcgttttc
     8101 ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc agatattggc
     8161 cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca tcgagccagc
     8221 cgttacgcca ggcgatctct tccaggcagg caatcttaaa gccctggcgt ttttctactg
     8281 tctgcacaaa agtgctggcc tcaatcaggc tgtcgtgagt gccggtatcc agccaggcaa
     8341 atccgcgccc gagcagttca acggtcaggt tgcccgcctc gaggtacatc tggttgatgg
     8401 aggtaatctc cagttcaccg cgctccgacg gcttcacctg ctttgcgtac tccacgactt
     8461 tactgtcgta gaaataaagc ccggtcaccg cccagtttga cttcggctgt tttggctttt
     8521 cttcaagcga gatagcgcgg aaattgtcgt caaattccac cacgccaaag cgttccgggt
     8581 ccatcacctg atagccaaaa accgtcgccc cttccgtgcg cgccgcaaca tgacgcagct
     8641 tcggactgaa gccctgaccg aagaagatgt tatcgcccag caccagacaa gaaggttcac
     8701 cattaaggaa ggtttcaccg atgataaagg cctgcgccag accgtccggg ctgggctgtt
     8761 cggcatattc cagctggata ccgaactcac taccatcgcc cagcaggcgc tggaaataac
     8821 ctttatcttc cggcgtagtg atgatgagaa tttcgcggat accggccagc atcagcaccg
     8881 acagcggata gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc gatacgccgc
     8941 gcgtaatcgg atgcaatcgg gtaccggaac cgcccgccag gataataccc ttcatgtttg
     9001 cctccgctga aaattagcct ttcagaccta aacgctcacc ctgatagctg ccgtcctgca
     9061 cctgcttcca ccagctttca ttagccagat accactgaac cgttttacgc attccacttt
     9121 caaaggtttc ctgcggcagc cagccaagtt cacgggcaat tttcgaagca tcaatggcat
     9181 aacgcagatc atgccccgga cggtcagcga caaaggtgat caggtcacga tagtacgcca
     9241 cgccgtgcgg cttgttcgaa gccagttctt ccagcagctc gcaaatggtt tccacaacat
     9301 cgagattctt acgctcgttg tgaccaccaa tattataggt ttcaccgact ttcccggtgg
     9361 tcgccacgca atacagcgcg cgggcgtgat cttccacata cagccagtca cggatttgct
     9421 gcccgttgcc atataccggc agcggtttac ccgccagcgc gttgaggatc atcagcggga
     9481 tcagtttttc cggaaagtgg taaggaccgt agttattcga gcagttggtg atcagcgtcg
     9541 gcagaccgta ggtccgcagc caggcgcgca ccagatggtc gctgctggct tttgacgcgg
     9601 aataagggct gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc gtcgagtgca
     9661 ggtcaccata tacttcgtcg gtggagatat gatgaaaacg gaacgctgat tttttatctt
     9721 ccgtcagcgt attccagtaa gcccgcgccg cttcaagcaa tgtataagtc ccgacaatgt
     9781 tggtttcaat aaacgctgcc gggccgtcaa tagaacggtc aacatggctt tcggctgcca
     9841 gatgcatgac acagtctggc tgatgctcag tgaatacgcg tgccagttct gcccgatcgc
     9901 agatatcaac tttctcaaag gcaaagcgct cgctttgcgc gaccggtgcc agcgacatca
     9961 ggtttccggc gtaggtcagc ttatcgacca ctaccaccgc atcgctcgtt tcgttgatga
    10021 tataacgcac cagcgccgag ccaataaacc cggcaccacc tgttatcaga atttttctca
    10081 tcagcgccag actcctttgg catcgacgac atactgctga tggacattgt cgccattgat
    10141 aactttgaac tgactatgat cgaccagcat caccagcacg tctgccgttg ccagcgcctc
    10201 gtcaagctgc gccagagtac aaagcccggt cagttttttc ggcaactggt ggatgttagg
    10261 ctcaacaacc agcgtttcgc cgctgtgcca ctgggcgatc agttcagcga tttccatcgc
    10321 cgggctttcg cgcaggtcat caatattcgg tttaaacgcc agaccaaagc aggcgatttt
    10381 cagttcactg gctcgtttat cggtagcagc aaggcagtcg gcaaccgccg ctttcacctg
    10441 atcgataacc cagaacggtt tgtgatcgtt aacttcgcgc gcggtacgga taagccgcgc
    10501 ctgctggggg ttctgtgcca cgataaacca cggatcaaca gcaatgcagt gaccgcccac
    10561 gccagggcca ggctgaagaa tattaacgcg agggtgacga ttcgccaggc gaatcagttc
    10621 ccagacgtta atcccctgat cggcacaaat cagcgacaat tcattagcaa aagcgatatt
    10681 cacatcgcgg aagctgtttt cggtgagctt acacatttcc gccgtccgcg agttagtgac
    10741 gacacactca ccttcgagga aaattttgta caattcgctg gcgcgggccg aacaaaccgg
    10801 cgtcatacca ccaatcacgc gatcgttttt aatcagctcg accataacct ggcccggtaa
    10861 tacacgttcc gggcagtaag caatgttgac gtccgcctgc tcgcccacct gctgtgggaa
    10921 agtcagatcc gggcgcatct ctgctaacca ttctgccatc ttctcggttg accccaccgg
    10981 cgaggtggat tcaaggatca ccagcgcgcc ttttttcagc actggcgcaa tggagcgagc
    11041 agccgattca acgtaggtca tatctggctc atgatcgcct ttaaacggcg taggtacagc
    11101 aatcagccag gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac cgccttctac
    11161 ggcagttttt actacgctcg ccaaatcagg ctcgacgata tggatttcgc cacgattgat
    11221 ggtatcaacc gcgtgttggt tgatatcgac accaatcacc tgtttttgcc gtgaggcaaa
    11281 cgctgctgct gtaggcagcc cgatgtaacc cagtccgata acagaaatgg tcgcaaaact
    11341 catagtgata tccgattatt ttttaacgct tccagaatgc gagagcatgc ctgaccatca
    11401 ccatacgggt tatgggcgcg gctcatagct tgatattcgt tttcgtcttt taaaagacgc
    11461 gtcacttcct cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt acccgccgtc
    11521 accgcttccg gacgctcagt ggtatcgcgc atcaccagca ccggtttccc cagcgaaggc
    11581 gcttcttcct gaatgccgcc tgagtcagtc aaaatcagcc aggcgtggtt catcagccag
    11641 acaaacggta aatactcctg gggatcgatc agaatgacat ttttcacatg ccccagaatg
    11701 cgattgaccg gctctctgac gttcgggttg agatgcaccg gatagacaat ctggatgtcc
    11761 tggtgcgtgg tggcgatgtc tgccagcgcg tggcagattt cttcaaagcc acgaccgaaa
    11821 ctctcacgcc tatgaccggt caccagaatc atctttttat cgggggcgat aaacgggtaa
    11881 tttgccgcca gttctgaacg cagcgtgtcg ctgctcatca cctggtcacg cacccataac
    11941 agtgcatcaa tgactgtatt accggtaatg aagattcggc tatccgcaac gttttcacgc
    12001 agcaagtttt gccgggaagt ttcggttgga gagaagtgat acatcgccag atgcccggtc
    12061 aatgtacggt tagcctcttc cggccacggc gaatagagag cgcccgtgcg cagaccagcc
    12121 tcaacgtgac caacaggaat acgctgataa aacgccgcca ggctggttgc cagcgtcgtc
    12181 gtcgtatcgc cgtgaaccag cacgacgtct ggtttgaact cggcaagaat aggttttagc
    12241 ccttccagaa tccgacaggt tatctctgtc aggccctgtc ctggctgcat tatgttgaga
    12301 tcgtagtcag gtacaatgga aaagagtttc agtacctgat caagcatctc ccgatgctgc
    12361 gcagtgacgc aaactttagc ctcaaaaaaa ggatcttttg ccaacgcatg caccaacgga
    12421 gccatcttga tggcttccgg gcgcgtacca aatacagtca gtactttcac atcgattctc
    12481 ttcgaataag cggcgagcgc ctttgcgctc accgcagcag tgttgctatt tcgagcaacg
    12541 gcgggttaat gcgacaccag ccccgatcag ccccccgaca atgccccaca taatcatcag
    12601 gaaggcacga cgtgggctat cgcgttttac cggttcttcc ggcgtacgca aatagcgata
    12661 ggtctgaaaa cgcggatcca gggttggacc aacattcagg gtgtttaaca tggctcgatt
    12721 ctgatcatag tcgagatcaa aggccggacc gacggcctgt aaattttcca gtcgagcctg
    12781 gagcattgga cgcccaagca ggaacatttc tgaatcaggt aattcctcgg caggcacatc
    12841 tgtcgcactg cgcgaaatat tatgctgctc agcaattttc agcgcctgct caatactgtt
    12901 catccggcgg tcgtagatgg ctttcgccac ctcttcctga cgcttcacct gagctttcat
    12961 ctggatggtg cgtgccgccc atgcgccttt cagctcatca ttcagatggc tggctgcacg
    13021 ctggctggca aaagcaacat actgacgtaa caggttatta gcgtcaggcg cggtttcggc
    13081 aataagcttc acgctgtcat tgaccgcgcg agtaaagtct ccggggataa actggatgtt
    13141 gttaatcatt tcatccagca acgccgcatc ggctttgctg ttgcccacca tccgctgttt
    13201 gtaatagtcg gtttgcagcc agaactctct gcgggtatcc cacgaggcca gttgcataac
    13261 aaactcttta taggcttcgt ccatgaccga tggttgatcg gcagaagcca tgtttgaacg
    13321 gacatccagg ttacgcaaaa attgctgctg cgagtaatat ccccccagca tattcaccgt
    13381 tggacgatcg gtaatcgccg tcgagctcca ctcctgacga gcaaaaaaag tatacgccag
    13441 cgcgattaac gcaaacgcca gccccatgcc aataatccat agcttcccag cccacaaggt
    13501 acgaaacaac ccacgaatat ccagttcatt ttcagcgtct tcggccggtt tcccaggcat
    13561 tggttgtgtc atcacatcct catttatttg gttaaattgg ggctgccacc acgatttcta
    13621 cgcagtctgc gttttacgcg cttaataaag cgagcaactt tccaggcacg cttaatgcaa
    13681 tatccataga ggaagaatgc tagcaaaaag agcaccagca tgacccactc cgggacaaaa
    13741 tgagaatatt ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag ggtaatcagc
    13801 acaaacgcct gacgggaagt aaacccggca cgcatgatca aatggtgaat atgctgacgg
    13861 tcaggagaga atgggctcat gcctttacgc aggcgacggt acataatcgc caccatatcc
    13921 attagcggaa tggcgattat ccacaaagcg gtaaccgggc tgatgggatg ggttttgccc
    13981 tgggtcgttt cgagcaggat ccagataacg gtaaaaccaa tcagcgtact gcccgcatca
    14041 cccataaaga ctttgtagcg gcgacccagg ataccaaggt taagcatgat gtatggcagg
    14101 atggcggcga tcatcgcaaa gcaccagatt gcgaggctgg tttgcccgtc gaaccacaaa
    14161 atcataccga ttgctgcaaa cgagacgcag gacaacccgc ccagcaagcc atcaatgcca
    14221 tcaaccatgt tgaacgcatt aatggccgcc cagacggcaa atagcgtcag gaagtaacca
    14281 aacggtccga gcaccatctc ccaggagcca aagatataac ccaggctact gagataaagt
    14341 ttgccgaaca ccatcataac aatgccaaca gcggcctgta tggtggcacg gatttttacg
    14401 ctgatatcaa aacgatcatc cagcgcgcca atgaaaacaa gcacaccggc acaagcgaga
    14461 tagagagatg catgcggaat atagtaatcg acaattccga acgtgaagca aatccctgcg
    14521 taaaccgaaa tccccccaac gagaggtatc aatccctggt gacgtttgcg gaagtttggt
    14581 ttatccacta aaccgacttt ttttgccacc ttacgggcaa aaaacagaaa cagtgtcgtg
    14641 aataaaaaaa tactgatgag atcagtactc actgtcagta aattcacaat gcatgctctc
    14701 agagaaaatt attagcagaa gtataaccac gaagaccttt attcagaagg gaaagtccgt
    14761 tccgaaccca ccagccctgt gcaaaaatca accatttgat atgcaaattg cataacatgc
    14821 cagcaaattc caggaatttt cctaacatcg ccagcgcgac attaagatta cagatataaa
    14881 agcaaaacgc cacgtaaaca cgtggcgttt ttggcataag acaaatttat gagcgtttca
    14941 tcatttcgaa gaaatcgtca ttggtcttgg tcatggccag tttattaatg aggaattcca
    15001 ttgcatcgat ttcgcccatc gggtgaatga ttttgcgcag gatccacatt ttctgcagtt
    15061 cttcctgagt cgtgagcagc tcttctttac gggtaccgga acggttgtag tcgatagccg
    15121 ggaagacgcg tttttcagcg atcttacgag agaggtgcag ttccatgttg cctgtacctt
    15181 taaactcttc gtaaataact tcgtccattt tagaaccggt atcgataagc gccgtcgcga
    15241 taatggtcag gctgccgccc tcttccacgt tacgcgccgc accaaagaag cgtttcggac
    15301 gatgaagggc attggcgtcc acaccaccgg tcaacacttt acctgacgcc ggaacaacag
    15361 tgttgtaagc gcgcgccaga cgagtgatgg agtcgagcag aatgataacg tctttcttgt
    15421 gctcaaccag gcgtttggcc ttctcgatca ccatttccgc aacctgaacg tggcgagatg
    15481 cgggttcgtc aaaggtagaa gcaacaactt caccttttac cagacgctgc atctcggtta
    15541 cttcttccgg acgttcgtcg atcagcagaa ccatcagcac acaatccggg tggttgtaag
    15601 caatgctctg agcaatgttc tgcagcagca tagttttacc ggctttcggc ggtgccacaa
    15661 tcagaccacg ctgaccacga ccgataggtg atgccagatc cagtacgcga gcagttaaat
    15721 cttcagtaga accgttacca cgttccatac gcagacgaga gtttgcgtgc agcggggtta
    15781 agttctcaaa gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta acttcgttaa
    15841 ctttcagcag cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta ccagagatgg
    15901 tatcaccagt gcggaggttg aaacggcgga tttggctagg ggaaacgtag atgtcatcag
    15961 gaccggcgag gtaggagctg tctgcggaac ggaggaaacc aaatccatcc tgcaatatct
    16021 ccagtacgcc atcaccaaag atatcttcgc cactctttgc gtgctgcttc aggatggcaa
    16081 aaataatgtc ctgcttacgc atgcgagcca ggttttccag ccccatattt tcgccgagag
    16141 tgatcagctc agaaaccggc gtattcttta attcggtaag attcatagtg gtgtgagttc
    16201 ttaaacttgg ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga tcatccatgc
    16261 ctgtttacgg tcatcgactc atgtcttttc gctgcctggt cacaggagag tacgcagaac
    16321 tgaaacgaca agacggattg agtgacgagc cagaaatctg ttcacttcgc atttaagata
    16381 aaacgggaag cgttggaaat tacaagattc aaacttaata aggtatgttt aatacgaagt
    16441 caacactaag ttagcatgac tcacgccggg cgtccagttt ttagcgacgg ggcacccgaa
    16501 catgaaattc ccttacgcca ggttagcgtc gaggaactct ttcaactgac ctttagacag
    16561 tgcgcccact ttggttgccg ccacttcacc gtttttgaac agcagcagag tcgggatacc
    16621 acggatgcca tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt
    16681 cagtttgccc tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg
    16741 accgcaccac tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt
    16801 gtcaaaactg tcgtcagtca ggtgaataat tttatcgctc atatataact ccacaggaat
    16861 aagcctggcg tgttggtttc gttgttggtg taacattaac caactaaagg ttgactttat
    16921 ttcaccggat acgctttcgt aaagcaatag taagctgata ttctaccaca ctatgagcaa
    16981 aacacattta acagaacaga agttttccga cttcgccctg catccgaagg ttgtagaagc
    17041 ccttgaaaaa aaagggtttc ataactgtac gcccattcag gcactggccc ttccgctgac
    17101 gctggcgggt cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt
    17161 tcttacgtca acgtttcatt atcttctctc tcatcctgcg attgccgatc gcaaggtgaa
    17221 tcaaccgcgt gccttaatta tggcaccgac gcgtgaactt gccgtgcaga tccacgccga
    17281 cgcggaaccg ctggcggaag ctaccggcct gaagctgggc ctggcttacg gtggtgatgg
    17341 ctatgacaaa cagctgaaag tgctggaaag cggcgttgac attctgattg gcaccacggg
    17401 tcgtttaatt gactacgcca agcagaacca cattaacctc ggtgccattc aggtagtggt
    17461 actggacgaa gccgatcgca tgtacgatct aggctttatt aaagatatcc gctggctgtt
    17521 ccgccgtatg ccgcctgcaa atcagcgcct caacatgctg ttctccgcca cgctttcgta
    17581 ccgggtacgt gaactggcgt tcgagcagat gaacaatgcc gaatatattg aagtggaacc
    17641 ggaacagaaa acgggccacc gtattaaaga agagcttttc tacccttcta acgaagaaaa
    17701 aatgcgtttg ctgcaaacgc tgatcgaaga agagtggcca gacagagcga ttattttcgc
    17761 caataccaaa caccgttgtg aagagatctg gggccacctg gcggcagatg gtcatcgtgt
    17821 cggtttgttg acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac
    17881 ccgtggcgat ctggatattc tggttgccac cgacgttgcc gcgcgtggtt tgcatattcc
    17941 ggcagtgacg cacgtcttta actacgattt acccgatgac tgtgaagatt acgttcaccg
    18001 tattggtcgt acaggtcgcg caggcgcaag cggtcactct atcagcctgg cgtgtgaaga
    18061 gtatgcattg aatttgcctg ctattgagac ctatattggt cactcaattc cggtaagcaa
    18121 atacaatccg gacgcattga tgaccgatct gccaaaaccg ctgcgcctca cgcgcccgcg
    18181 cacaggcaat ggtccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt caggttaaat
    18241 aaaatgtcat actcaaaatc attcaaactg tatcaagatg gcaagagaat gaatcccgat
    18301 gagcttactg tagtaagtga ttcgggtgaa agaatgcagc caacacagag acagattgaa
    18361 ggatgaagag tatgggttcc acctcgtcgc tgtatgcagc cattgatctc ggttcgaata
    18421 gttttcatat gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg acgcgaatta
    18481 aacgcaaagt gcgtctggct gctggcctga atagcgaaaa tgacctctct aatgaagcaa
    18541 tggagcgcgg ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat atccctccct
    18601 cgcaaattcg cgttgtcgct acggcgacat tacgacttgc cgtcaatgcg ggtgatttta
    18661 ttgccaaagc gcaggaaatc ctcggttgtc cggtacaggt gatcagcggt gaagaggaag
    18721 cgcgtctgat ttatcagggc gttgctcaca ccactggtgg tgccgatcag cgcctggtgg
    18781 tggatatagg cggtgccagt actgaactgg taaccggcac gggtgcacaa accacctcgt
    18841 tgttcagcct gtcgatgggc tgcgtcacct ggctggaacg ctattttgcc gatcgtaatc
    18901 tggggcaaga aaattttgat gctgcagaaa aagcggcacg cgaagtgtta cgtccggttg
    18961 ccgatgaatt acggtatcac ggctggaaag tgtgcgttgg tgcttccggc accgtgcagg
    19021 cgttacagga aatcatgatg gcacagggga tggatgaacg cattaccctg gaaaagttgc
    19081 agcaattgaa acagcgagcc attcattgcg gtcggctgga ggaactggag attgacgggc
    19141 tgacgctgga acgtgcatta gtgttcccga gtggtctggc gatcctgatc gccattttta
    19201 ccgaactgaa tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa ggcctggtct
    19261 acggcatgtt gcatctggcc gtcgagcagg atattcgcag ccgtacactg cgtaatattc
    19321 aacgccgctt tatgatcgat attgatcagg cacagcgcgt agccaaagtt gcggctaact
    19381 tcttcgatca ggtggaaaac gaatggcatc ttgaagcaat aagccgcgat ttgctcatca
    19441 gcgcctgtca gcttcatgaa atcggcctga gcgttgactt caaacaagct ccgcaacacg
    19501 ctgcttatct ggtgcgtaac ctggatcttc ccggttttac ccccgcacag aagaaactgc
    19561 tggcgacgct actgctcaac cagactaacc cggtcgatct ctcatcgctg catcagcaaa
    19621 atgccgtacc accgcgcgtc gcagaacaac tctgccgttt actccgcctg gctatcattt
    19681 ttgccagccg tcgccgtgac gatctcgtgc cagagatgac attacaggct aaccatgaac
    19741 tgttgacctt gacgcttccg caaggttggc taacccaaca tccgctgggt aaagagatta
    19801 ttgctcagga aaaccagtgg cagagctatg tccactggcc gctggaagtg cattaatgag
    19861 taagtgccgg atgcgatgct gacgcatctt ctccggcctt gattatttcc ctcgttttgc
    19921 cgccatcatc gctttcagat tcgccagatg gctttgccct ttctgcatcc gttcttcggc
    19981 actgaccact ttgcgctcct gttcccaaat cagatcatcc tgcggcagct ccagcaaaaa
    20041 gcggctcggc tccgggcgca ccagttcgcc gtactgacgg cgttctttac acagcgtaaa
    20101 ggtcaattcc ttctgggcgc gggtaatgcc gacataggcc agccgccgct cctcatcgat
    20161 attatcttca tcgatactgc tctggtgcgg caaaaaccct tcttccatac cgaccatgta
    20221 gacataagga aactccagcc cttttgacgc gtggagagtc atcagttgca cctgatccag
    20281 ctcttcttca ctctcaccac gctccatcat gtcgcgtaaa gtaaagcgcg tcaccacctg
    20341 ggtgagcgtc atcggctcat ccagttcgct gccttccagc atctccgtca tccagctaaa
    20401 cagttggttg acgttcttca tgcgcatttc ggcagctttc gtgctgggcg atgtttcgta
    20461 cagccaggat tcataatcca tgccgtggat cagatcgcgc accgcggcaa tcggctcccg
    20521 ctccgccaga cgctggattt ctgccaacca gtgagtgaag cgggtcaatg cttcataacc
    20581 acgtccgcta agcgtctgac tcaggcccat atcaaagctg gcggtaaaca tgcttttatt
    20641 gcgcgtcatc gcccactcac ccagcttttt cagcgtcgcc gggccaatct ctcgcttcgg
    20701 cgtgttaacg atacgcagaa atgcgctgtc atcgtccggg ttagtcagca cgcgcagata
    20761 agccagcaag tccttgattt caggacgaga gaaaaacgac gtaccaccag aaattttgta
    20821 cgggatgcgg ttttgcatca ggaatttttc aaacacccgc gactgatagt taccgcgata
    20881 aagaatagcg taatctttgt actgcgtttt attgacgaag tgatgcgcaa tcagttcacc
    20941 agtgacgcgc tcggcttcat gttcttcgtt attcgcgctt aaaactttta gctctgtgcc
    21001 gtaacccagt tcggagaaca gacgcttttc aaagacgtgc gggttattgg cgatcaggat
    21061 gttcgccgct ttcaaaatac gtccggaaga gcggtagttc tgctcaagct taatcacctt
    21121 cagcgccgga aaatcctggc tcagcagcac caggttttgc ggacgtgcgc cgcgccagga
    21181 gtagatcgac tggtcatcgt cacccaccac ggtaaagcgc gcgcggctgc ccaccagcag
    21241 tttcaccagt tcatactggc tggtgttggt gtcctgatat tcgtccacca gcaggtagcg
    21301 aattttgttc tgccagcgct cgcggacttc ttcattgcgt tgcagaagta gcgtcggcaa
    21361 taaaatcaga tcatcgaagt cgagcacatt acaggctttc aggtgtgcat catacagccc
    21421 ataacaatgg gcaaaaatac ggtcccgctc gccaatcgca ctggctgccg cctgggacgg
    21481 tgttttgaga tcgttcttcc agttagagat ggtcgaaatc aactgttgca ggagaacttt
    21541 gtcatcttca atcagccctt cggtcaactc tttgagcaac gccagctgat cggtatcgtc
    21601 aaacagcgaa aagttcgcct tcatccccag cgccgcatac tcacgcttga tgatatccag
    21661 ccccagcgtg tggaaggttg agatcatcag cccatgcgcc tctttgcgac ccagcgtctg
    21721 tccgacacgc tctttcatct cgcgcgccgc cttattggta aaggtcaccg ccgcgatatg
    21781 tcgcgcctga taaccgcacc cgcggatcag atgggcgatt ttattggtga taacacgagt
    21841 tttaccggaa cccgcacccg ccagcaccag gcaggggccg gtaacgaatt cgacagcttg
    21901 ttgttggccg gggtttagac gcataggaaa atcgctcaat gaaagtcggg aggggaaaat
    21961 aacagcgtgg tagtatagcc agcctcatcc ttaccactca aggcacgatc atggcaaaaa
    22021 cagcagcagc actgcatatc cttgtaaaag aagagaaact ggctctggat cttctcgtgc
    22081 agattaagaa cggtgccgat ttcggcaagc tggcgaagaa acactccatt tgcccatcag
    22141 gcaaacgcgg cggtgattta ggtgaattcc gccagggtca gatggttccg gcgttcgata
    22201 aagttgtgtt ctcttgtccg gtactggagc caaccggccc gctgcacacc cagttcggtt
    22261 accacatcat taaggtgctg taccgtaact aatagcaagg ccttctccgg gagaaggcct
    22321 tgaactctga atcgctatta acccgcaaca gcaatacgtt tcatatccgt catatagccg
    22381 cgcagtttct tacctacttg ctcaatcgca tggctgcgaa tcgcttcatt tacatcacgc
    22441 agctgcgcgt tatctaccgc accttccgga atagctttac ccaaatcgcc cggttgcagt
    22501 tctgccataa acggtttcag cagcggcaca caagcgtaag agaacagata gttaccgtac
    22561 tcggcagtat cagagataac cacgttcatt tcatacagac gcttacggac gatggtgttg
    22621 gcaatcagcg gcagttcgtg cagtgattca tagtaagcag attcttcgat gatgccggaa
    22681 tcaaccatgg tttcgaacgc cagttcaacg cccgctttca ccatcgcgat catcagtacg
    22741 cctttatcga agtactcctg ctcgccgatt ttgccttcat actgcggcgc ggtttcgaag
    22801 gcggttttgc cggtctcttc acgccaggtc agcagtttct tatcatcgtt ggcccagtcc
    22861 gccatcatac cggaggagaa ttcgccggag atgatgtcgt ccatatgttt ctggaacagc
    22921 ggtgccatga tctctttcag ctgttcagac aacgcgtaag cacgcagttt cgccgggtta
    22981 gagagacggt ccatcatcag tgtgataccg ccctgcttca gcgcttcggt gatggtttcc
    23041 caaccgaact gaattagttt ttctgcgtat gccggatcgg tgccctcttc caccagcttg
    23101 tcgaagcaca gcagcgagcc agcctgcaac ataccgcaca ggatggtttg ctcacccatc
    23161 aggtcagatt tcacttccgc aacgaaggac gattccagca cgcccgcacg gtgaccaccg
    23221 gttgctgccg cccatgcttt agcgatcgcc atgccttcgc ctttcggatc gttttccggg
    23281 tgaacggcaa tcagcgtcgg tacgccgaat ccacgtttgt actcttcacg cacttcggtg
    23341 ccagggcatt tcggcgcaac catgactacg gtgatgtctt tacggatctg ctcgcccact
    23401 tcgacgatgt tgaaaccgtg cgagtaaccc agcgccgcgc cgtctttcat cagtggctgt
    23461 acggtgcgca ctacatcaga gtgctgcttg tccggcgtca ggttaaccac cagatccgcc
    23521 tgtgggatca gttcttcgta agtacccact ttaaaaccat tttcggtcgc tttacgccag
    23581 gacgcgcgct tctcggcaat cgcttcttta cgcagagcgt aggagatatc gagaccagaa
    23641 tcacgcatgt tcagaccctg gttcagacct tgtgcgccac agccgacgat gactactttt
    23701 ttaccctgaa ggtagctcgc gccatcggcg aattcatcgc gccccataaa gcgacatttg
    23761 cccagctgtg ccagctgctg acgcagattc agtgtattga agtagttagc catggtgatt
    23821 cctcgtgatg ttgtgcttct tattgttcgg ttcgcgtttg cgagattgaa ttcactatat
    23881 gacaggaaat ttattgcgga aattgatata ttcacaacgt cacattgcaa tttctgcaac
    23941 gtcaacattg agggctgcgc cattggattt acgcgatctg aaaaccttcc tgcatctggc
    24001 ggaaagccgc cattttggcc gcagcgcgcg ggcgatgcac gttagcccgt ccacgctctc
    24061 acggcagatt cagcgcctgg aagaagatct cggccagccg ctgtttgtgc gcgataaccg
    24121 cacggtgacg ctgactgaag cgggcgaaga actgcgcgtt ttcgcccagc aaacgctgtt
    24181 gcagtatcag cagctgcgcc acaccatcga ccagcaaggg ccgttgctct atggcgaatt
    24241 acatatcttc tgctcggtga ccgccgctta cagccatctg ccgccgattc tggatcgctt
    24301 ccgcgcggaa cacccgtcgg tggagatcaa actcactacc ggtgatgcgg cagatgcaat
    24361 ggaaaaagtc gtcacaggtg aagctgatct ggcgattgcc gggaagccag agactctgcc
    24421 cggcgcggtg gcgttttcga tgttggagaa tctggcagtg gtgttgattg cacccgcatt
    24481 gccctgcccg gtgcgtaatc aggtttctgt cgaaaagccg gactggtcaa cggtaccgtt
    24541 tatcatggcc aatcaggggc cggtgcgccg ccgcattgag ttgtggttcc gacgcaataa
    24601 aatcagtaac ccgatgattt acgccacggt tggcgggcat gaagcgatgg tatcgatggt
    24661 ggcgctcggc tgtggcgtgg cattgttgcc ggaagtggtg ctggaaaaca gccccgaacc
    24721 ggtgcgtaac cgcgtgatga ttttagagcg cagcgatgag aaaacgccgt ttgagctggg
    24781 cgtgtgtgcg cagaaaaagc ggctacatga gccgctaatt gaggcattct ggaagatttt
    24841 gccgaaccat taacccgcca aaaagaacct gaacgccggg ttattggttt cgtcgtggca
    24901 atcgtagccc agctcattca gccgggtttc gaaatccggt tcatggtcgc caagttcgaa
    24961 cgccgccagt acgcgcccgt agtcggtgcc atggctgcga tagtggaaca aagaaatgtt
    25021 ccagtgcgta cccagcgtgt tgaggaagcg cagcagcgcg cccggtgatt ccgggaattc
    25081 gaagctgtag aggcgttcct gcaacggatg tgacggacgc ccgccgacca tatagcgcac
    25141 atgcaacttc gccatttcgt cgtcggagag atccaccacg ctgtagccgc cgtcgttgag
    25201 catctgcaaa atttctttgc gctcttcgag gccgcggctc aggcgcacgc cgacaaagat
    25261 gcaggcgttt ttggcatcgg caaaacggta gttgaactcg gtgaccgatc gcccgccaag
    25321 cagttggcag aatttgagga agctgccttt ttcttccgga atggtcaccg ccaacaacgc
    25381 ttcacgctgt tcgcccagtt cgcagcgttc cgagacgtag cgcaggccgt ggaagttcac
    25441 gttggcaccg gaaagaatat gcgccagccg ttcgccgcga atgttgtgct gggcgatata
    25501 ttttttcatt cccgccagcg ccagcgcgcc agagggttcc gccaccgcac gcacatcttc
    25561 gaataaatcc ttcatcgccg cacagatcgc atcgctatcg acggtgatga tgtcgtcgag
    25621 atactcctgg cataaacgga aggtttcgtc accgatgcgt tttaccgcga caccttcggc
    25681 gaacagtccc acgcgtggca gatcaaccgg atgacccgca tccagcgctg ctttcaggca
    25741 ggcggagtct tccgcttcta cggcgatcac tttgatttgc ggcatcagtt gtttgatcag
    25801 caccgctacg cccgccgcca gaccgccgcc gccgactggc acaaatacgc ggtcgagatg
    25861 ggcgtcctgc tggagcagtt ccagcgccag cgtgccttgc ccggcaatca ccatcggatg
    25921 gtcgaacggc ggcacccagg tgaacccctg ctgctgtgac agttcgatcg ctttagcttt
    25981 cgcttcgtca aagttcgcgc catgaagcag cacttcgccg ccgaatccac gtaccgcatc
    26041 gactttgatg tcggcggtgg cggttggcat aacgatcagg gccttcacgc ctaaccgcgc
    26101 agaagaaaac gcgacgccct gcgcgtggtt acccgcagaa gcagtgatca cgccgtgcgc
    26161 tttttgttct tccgtcaggc ccgccatcat ggcgtatgcg ccgcgcagct taaagctgtg
    26221 cactggctgg cgatcttcgc gcttcaccag aatgacgtta tcaagacgcg acgacagttt
    26281 ttccattttt tgtagcggcg taacctgcgc cgcctcgtaa accggcgcgc gcagcactgc
    26341 tcttaaatat tcggcacctt ccggagcacc ggacaggggt tgcgagtcag ccattattaa
    26401 ccccccagtt tcgatttatc gcgcaccgca cctttgtcgg cgctggttgc caggctggcg
    26461 taggcgcaca gggcaaagga aacctgacgt tcacggtttt tcggcgtcca ggctttgtca
    26521 ccccgagctt cctgcgcttc acgacgcgcc gccagttcgg catcgcttac ctgtaactga
    26581 atgccacggt tcgggatgtc gatagcgatc aggtcaccat cttcaatcag accaatgctg
    26641 ccgccgcttg ccgcttccgg tgagacgtgg ccgatggaaa gaccagaggt gccgccagag
    26701 aaacgaccgt cggtgatcag cgcacaggct ttaccgagac ccattgattt caggaagctg
    26761 gttgggtaga gcatttcctg catgccaggg ccgcctttcg ggccttcata gcggatcacc
    26821 accacatcgc ccgcgacaac tttgccaccg aggatcgctt ctaccgcgtc gtcctggctt
    26881 tcgtacactt tcgccgggcc ggtgaatttg aggatgctgt catcgacgcc cgcggttttc
    26941 acgatgcagc cgttttcagc aaaattaccg tagagcaccg ccaggccgcc gtctttgctg
    27001 taggcgtgtt ccagcgagcg gatacagccg tttgcgcgat cgtcatcgag agaatcccaa
    27061 cggcaatcct gcgagaatgc ctgtgtagta cgaatgcccg cagggcctgc gcggaacata
    27121 ttttttaccg cgtcatcctg ggtcagcata acgtcgtatt gttccagagt ttgcggcaac
    27181 gtcaggccaa gtacgttttt cacatcacgg ttcagtaacc ccgcgcgatc cagttcaccg
    27241 agaataccga taacaccacc agcacggtga acatcttcca tatggtattt ttgggtgctc
    27301 ggcgcaactt tgcataactg cgggacttta cgcgaaagtt tgtcgatatc actcatggtg
    27361 aagtcgattt ccgcttcctg cgccgccgcc agcaggtgaa gtaccgtgtt ggtagagccg
    27421 cccatcgcga tatccagcgt catggcattt tcaaacgccg ctttgctagc aatattgcgc
    27481 ggcagtgcac tttcgtcgtt ttgctcgtag taacgtttgg tcaattcaac aatgcgttta
    27541 ccggcattga ggaacaactg cttacggtcc gcgtgggttg ccagcagcga gccgttgccc
    27601 ggctgcgaca ggcccagcgc ttcggtcagg cagttcattg agttagcggt aaacatcccg
    27661 gagcaggagc cgcaggtcgg acacgcggaa cgttcaacct gatcgctctg ggagtcagaa
    27721 actttcgggt ctgcgccctg gatcatcgca tcaaccagat cgagtttgat tatctgatcg
    27781 gacagtttgg ttttcccggc ctccatcggg ccgccggaaa caaagatcac cggaatattc
    27841 aggcgcaggg aagccatcag catccccggg gtgattttgt cgcagttaga gatgcagacc
    27901 atggcgtcgg cgcagtgggc gttgaccata tactcaacgg aatcagcgat cagttcgcga
    27961 gatggaagtg aataaagcat ccccccgtgg cccatggcaa tcccatcatc caccgcaatg
    28021 gtgttgaact ctttggcaac gccgccagcc gcttcaattt gttcggcgac cagtttaccg
    28081 agatcgcgca gatggacgtg acccggtaca aattgggtga acgagttcac aaccgcgata
    28141 atcggcttgc caaaatcggc gtcggtcatt ccggtggcgc gccacagcgc acgagcaccc
    28201 gccatattac gaccatgagt ggtggtggcg gaacggtact taggcatact ttatttactc
    28261 ccagtgtctg tctcgtaaat gggacggtgc gtgccgtccc attttttgta tttattgatt
    28321 aacttgatct aaccagcccc atttatcttc ggtttcgcca gtgaagaggc cgaagaaggc
    28381 ttgctgaatg cgtttggtaa ccgggccaca acggccttcg ccaatctgaa taccgtctac
    28441 gctgcgcact ggcgtgattt ctgccgccgt accggacata aagacttcat ccgccaagta
    28501 cagggattcg cgcgacagca cctgctcacg cacttcaatt cccagctctt tcgccagttt
    28561 gatgatggca tcacgggtaa tacccggcag cgcggaggag gtgaacggtg gggtgaacag
    28621 cacaccatct ttcacttcaa acaggttttc gcctgcgcct tcagagatat aaccgttcac
    28681 atccagcgcg ataccttcct gataaccgtg gcggcgcgct tcgctaccca ccagcaggga
    28741 agagagatag ttaccaccgg cttttgccgc agttgggatg gtgtttggtg ctgcgcggtt
    28801 ccaggaggaa accatcgcat cgatcccctg ctccagcgct tctgcgccca gatacgctcc
    28861 ccacgggaaa gcggcgataa tcacatcggt tgagtatccc gctggcgggt ttactcccat
    28921 gccaacatca ccgacgaaga tcagcggacg gatataggcg ctggtgagat tgtttttgcg
    28981 gatcacgtca cgacaagctt ccatcagctc atcaatgctc tgtgaaaccg ggaagcgata
    29041 gattttggcg gagtcatgca gacgctgcat atgctcacga tggcggaata caaccggtcc
    29101 tttgtgtgag tcgtagcaac ggatgccttc aaaaaccgag gtgccatagt gcagcgcgtg
    29161 cgacatcaca tgcaccttcg cgtcttccca gcgaaccatc tccccattga accaaatgta
    29221 atcagctttc ttcgtggtca tttttatatt ccttttgcgc tcaggcgcgg atttgttgtg
    29281 atgtggttgt gctctggcag atggcaacgt gtgcgacgtc caccagttta tttaactgac
    29341 taaacagtaa gtcgaccgac cgtgggctgg caacggtcaa ttcgatattt atattttgtg
    29401 catcgctggc ggcagccata ttcattgagc agacgtggaa accacgatga cgcaccacgc
    29461 gtaaaacacg ttctaaggtt tccggattga agcgagccga tacattgacc tgatgttgca
    29521 tcatgataat ttctccaaca tttctgaatt actggcacct ggcggcacca gcggccagac
    29581 gttctcaagt tcgtcgattg agacatgaag caggtatggc ccatcactgt tcagcatggt
    29641 gtcgagtgcc gcttcaacct ggtctttacg ggtgatgtgt tggccaggga tgccgaaggc
    29701 gctggctaac atgaggaaat cggggttatc ggtaagggtg gtttcgctgt atcgttcctg
    29761 aaaaaacagt tgctgccatt gtcgaaccat ccctaaccgt tggttatcga gtaagacgat
    29821 tttcaacggt aactgcttgc gttttacggt gcccagctct tgcacattca tcatgaaaga
    29881 gccgtcaccg gagatacaga caacggtatc gttcggtcgc gcgacttgtg cgccaactgc
    29941 cgccggtaaa ccaaaaccca tggtacctaa gccgctggag gtgatgaaat tttccgggcg
    30001 agtgtgggcg atgtgctgcg cagcccacat ctggtgctgc cccacatctg tggtcacgac
    30061 gcaatccgca ggtttacgat ccgacagttg ttttaacaac aacggagcgt agatagcgtc
    30121 accgggatgg tcgtaacgcc aggaatgttc atcacgcagc tgcgcgcagt gttgctgcca
    30181 gtcattgata tttaacggct gctgtaatgc tggtaacaga gcatttaaat caccttgtaa
    30241 tgccacatgt gcctgacgca gcttgttcat ttctgccggg tcgatatcca tatggataac
    30301 actggcgtgt ggtgcgaagg tgttcagttt gccggtcacc cggtcatcaa aacgtgcacc
    30361 cacggcgatc agcaggtcac actcctgcac cgcgaagttt gccgctttgg ttccgtgcat
    30421 ccccagcatg cccagatagt acggataatc tgcttctact gcgcccagcc ctttcagcgt
    30481 acaggtggca ggcatttttg tggcagcgag aaattcacgc aaagccggaa ctgcctgcgc
    30541 catccccacg ccaccgccaa cgtacagcat cggtttttgc gcttttgcca gcatctggcg
    30601 cgcttgctcg acttcggcat gtgggaaagt cacttcgttt tcaacggtgg tgaaccacgg
    30661 ttccagatcg ccgctggcta actggatatc ttttgggata tcgaccagaa ccggaccagg
    30721 acgacctgag ctggcaacgt cgaatgcttc agccatgatg cgcggcaact cttccagaga
    30781 ctgcaccaga aagctgtgct tggtacaggc taacgacaat cccaggacat ccacttcctg
    30841 aaatgcgtcg gtgccgataa acggtgcgga cacctgacca gtgatggcaa cgacagggat
    30901 ggaatctaac agtgcgtccg caagcccggt tatcaggttg gttgcgcccg gaccagacgt
    30961 ggcgatacat acgccagttt taccggtagc acgggcataa ccgatagccg ccattgccgc
    31021 accctgctca tgtcggcaca gcaagtgctc cacgccgccg tcatacaatg catcgtaaac
    31081 cggcataatt gcgccacccg gataaccgaa aacggtgttc acaccctgtg cccgcaacgc
    31141 atgtaccacc cactgtgcgc cattcatagt tagttccccg tcctgaatct tgagaaacag
    31201 aattttgtgc tgttattcat tgtctgctcc tcggttatgt ttttaaggtc aaaaaaaccc
    31261 ccggaccttt cggtgcgggg gtcttagttc gttaaggctt gatctctaag cctttcctcg
    31321 tccaagtgca gccccgcacg gtgggataat aatcaccacc acgctaatca cgaccaggct
    31381 aatcactcgt agaagggctg tcattttgtc ttttcttgca tcttgttcga aggaatgcct
    31441 aaagagttac catagatttt gcgaatagca caagatattt ttgggcctat ttttatgatg
    31501 gctttaataa tttcaataga aattgttgtt ttttaaaaag aaaatagtga gtgaaaaaat
    31561 tcaatttcag tataaatgtt gttcccttcc tcgtagttca tcctttgtcc gctgcatttt
    31621 tgcgagcatc attccaccgc cagaacaact caacttcact tgcacaaata aacaggaatc
    31681 agccactaaa attcacgaat tagcgggcta cagcagcgat atcttatgca cataatggtg
    31741 actcaaggag ggtttatgtc actgtcaatt gttcataccc gcgcagccct ggaagtaaat
    31801 gcgcccccaa tcactgttga ggtacatatc agtaaaggtc tacccggctt aacgatggtg
    31861 ggcttaacag aaacaacggt gaaagaagct cgcgatcgcg tgcgcagcgc cattatcaat
    31921 agcggatatg aatatccggc gaaaaaaatc accatcaacc ttgctccagc cgatctgcca
    31981 aaagaagggg gacgatatga tttacctatc gccattgcgt tgctggcggc ctcagaacag
    32041 cttacagcca ataagttaga tgaatatgaa ttagtcggag aactagcgct tacaggcgct
    32101 ctgcgtggcg ttcccggtgc aatctccagt gcaactgaag ctattaagtc gggcagaaaa
    32161 attatcgtcg cgaaagataa cgaagatgaa gtggggctaa ttaacggtga aggatgcctg
    32221 atagccgatc atctgcaggc tgtctgtgcg tttctggaag gtaagaacac tctcgaacgc
    32281 ccgaaaccaa ctgatgcagt atcccgggcg ctacaacatg atctcagtga tgttgtcggt
    32341 caggagcaag gaaaacgagg actggaaatt accgccgctg gtgggcacaa ccttttactg
    32401 attgggccgc cgggaacagg taaaacaatg ctcgccagcc gtattaatgg cctgttgcca
    32461 gatttaagca atgaagaggc actggagagc gctgcgatat taagtctggt aaatgctgaa
    32521 tcagtacaaa aacaatggcg gcagcgcccg ttccgctcac ctcatcacag tgcgtcgtta
    32581 actgcgatgg tgggcggtgg tgcaattcca gggccaggtg aaatttcgtt ggcgcataac
    32641 ggcgtgcttt ttcttgatga gctacctgaa tttgaacggc gtacactgga tgccttgcga
    32701 gagccgattg aatccgggca gatccatctt tcacgcacac gagcaaaaat aacctatcca
    32761 gcccgtttcc agcttgttgc ggcgatgaat cccagcccta ccggacatta tcagggaaac
    32821 cataaccgct gcacgccaga acagacatta cgttatctca ctggagatcc cattaccacc
    32881 ccccggcatt ttgagtaaaa cggtagtgcc gggagaaagc agcgccaccg ttaaacaacg
    32941 cgtaatggcc gccagagagc gccaatttaa gcggcagaat aaactgaatg cctggctgga
    33001 tagtccggaa atacgccaat tctgcaaact tgagagcgaa gatgcgatgt ggttggaaga
    33061 aacgctgatc catctggggt tatcgattcg tgcctggcag cggttactga aagttgcacg
    33121 aaccattgct gatattgatc agtctgacat tatcacacgt cagcatttgc aggaggcagt
    33181 tagctatcgt gcgattgacc gtttgctcat ccatctgcaa aaactactga cataaaaaaa
    33241 gggcatttcg ccctttttat taatcgtcag aatcggtgta gtcttcagca ccttcaacct
    33301 gcggtttacc gccggaaagg gtgtgaaaac gttttggacg cttaatacgc gtcatatact
    33361 tggaccacac gcgttctgct tctgtcactg gctcacgttc gccacggcat actgctacga
    33421 agagtttctc ttcctcggta accggctcgc gtttgccaag atccaactca ttgaaggcat
    33481 aaccatgacg ctcaagcagt tgtgcctctt tgatggtgaa atcaccatga cgagagaatc
    33541 cacgtggata atgtttattg tcgaaatatc gattagtcgt cgtaaagctt tccgccatcc
    33601 tgcacgctcc taattctttg accgagctag ttatggcgcg gagtattagt tacgcttgac
    33661 agagtgtaaa acaaaacatt taaatcataa cgacaaataa ttttgcggag agcactgtgg
    33721 atacggaatt gttaaaaact ttcctggaag ttagccgaac gcgtcacttt ggtcgagcgg
    33781 ctgaatcgct ctatctgacc cagtcagcag tgagctttcg aatcagacaa ctggaaaatc
    33841 aactgggtgt gaaccttttc acccgccaca gaaacaatat ccgtttaacc gctgccggtg
    33901 aaaaactact gccttatgca gaaacgctca tgagcacctg gcaggccgcc cgcaaggagg
    33961 tggcgcatac ctcacgacat aacgagtttt ctatcggtgc cagcgcctcg ttgtgggaat
    34021 gtatgcttaa tcagtggttg ggacgcttgt atcaaaatca ggatgcccat acaggcttac
    34081 agttcgaagc gcgaattgcc caacggcagt ctctggtaaa acagctgcat gaacgccagc
    34141 ttgatcttct tattaccact gaagcgccca aaatggacga atttagtagt cagttgctgg
    34201 ggtatttcac tttagcgctt tataccagtg ccccttctaa actaaaggga gatcttaact
    34261 atctgcgact ggagtggggg ccagattttc aacagcatga ggcaggtttg atcggtgctg
    34321 acgaagttcc cattctgaca accagttctg ctgaactggc acagcaacag attgcgatgc
    34381 ttaatggttg cacctggcta cccgtcagct gggcgcgtaa aaaaggcggc ctgcataccg
    34441 ttgtcgatag cacaacactt tcacggccgc tttatgccat atggctgcaa aatagcgata
    34501 aaaatgcgtt gattcgcgat cttttgaaaa ttaacgtgct ggatgatgtg tattaatctg
    34561 aatggctggc aaggatgccg gtagaaggat ttacttcgga gaggattatt tcagataaaa
    34621 aaaatcctta gctttcgcta aggatgattt ctggcagggg cggagagact cgaactccca
    34681 acacccggtt ttggagaccg gtgctctacc aattgaacta cgcccctaat tagggtggcg
    34741 gaacggacgg gactcgaacc cgcgaccccc tgcgtgacag gcaggtattc taaccgactg
    34801 aactaccgct ccaccgaatt cttttacaac caccggtttt a
//
LOCUS       DAZPAB010000020        34622 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZPAB010000020 DAZPAB010000000
VERSION     DAZPAB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34622
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.020"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(72..1325)
                     /gene="amiC"
                     /locus_tag="ACHUPG_000908"
     CDS             complement(72..1325)
                     /gene="amiC"
                     /locus_tag="ACHUPG_000908"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR0860620.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1557..2888
                     /gene="argA"
                     /locus_tag="ACHUPG_000909"
     CDS             1557..2888
                     /gene="argA"
                     /locus_tag="ACHUPG_000909"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR0860621.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2950..4776)
                     /gene="recD"
                     /locus_tag="ACHUPG_000910"
     CDS             complement(2950..4776)
                     /gene="recD"
                     /locus_tag="ACHUPG_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR0860622.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4776..8318)
                     /gene="recB"
                     /locus_tag="ACHUPG_000911"
     CDS             complement(4776..8318)
                     /gene="recB"
                     /locus_tag="ACHUPG_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR0860623.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8311..11199)
                     /gene="ptrA"
                     /locus_tag="ACHUPG_000912"
     CDS             complement(8311..11199)
                     /gene="ptrA"
                     /locus_tag="ACHUPG_000912"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFR0860624.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11375..14743)
                     /gene="recC"
                     /locus_tag="ACHUPG_000913"
     CDS             complement(11375..14743)
                     /gene="recC"
                     /locus_tag="ACHUPG_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR0860625.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14756..15106)
                     /locus_tag="ACHUPG_000914"
     CDS             complement(14756..15106)
                     /locus_tag="ACHUPG_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR0860626.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(15091..15498)
                     /locus_tag="ACHUPG_000915"
     CDS             complement(15091..15498)
                     /locus_tag="ACHUPG_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR0860627.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(15495..16058)
                     /locus_tag="ACHUPG_000916"
     CDS             complement(15495..16058)
                     /locus_tag="ACHUPG_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR0860628.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16049..16519)
                     /locus_tag="ACHUPG_000917"
                     /pseudo
     CDS             complement(16049..16519)
                     /locus_tag="ACHUPG_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(16703..17497)
                     /gene="thyA"
                     /locus_tag="ACHUPG_000918"
     CDS             complement(16703..17497)
                     /gene="thyA"
                     /locus_tag="ACHUPG_000918"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR0860629.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(17504..18379)
                     /gene="lgt"
                     /locus_tag="ACHUPG_000919"
     CDS             complement(17504..18379)
                     /gene="lgt"
                     /locus_tag="ACHUPG_000919"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR0860630.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18530..20776)
                     /gene="ptsP"
                     /locus_tag="ACHUPG_000920"
     CDS             complement(18530..20776)
                     /gene="ptsP"
                     /locus_tag="ACHUPG_000920"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR0860631.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20789..21319)
                     /gene="rppH"
                     /locus_tag="ACHUPG_000921"
     CDS             complement(20789..21319)
                     /gene="rppH"
                     /locus_tag="ACHUPG_000921"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR0860632.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(21672..21818)
                     /gene="ygdT"
                     /locus_tag="ACHUPG_000922"
     CDS             complement(21672..21818)
                     /gene="ygdT"
                     /locus_tag="ACHUPG_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR0860633.1"
                     /translation="MLSTESWDNCEKPPLLFSFTALTCDETPVFSGSVLNLVAHSVDK
                     HGIG"
     gene            22004..22693
                     /gene="mutH"
                     /locus_tag="ACHUPG_000923"
     CDS             22004..22693
                     /gene="mutH"
                     /locus_tag="ACHUPG_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR0860634.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22762..23475
                     /locus_tag="ACHUPG_000924"
     CDS             22762..23475
                     /locus_tag="ACHUPG_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR0860635.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23613..23831
                     /gene="ygdR"
                     /locus_tag="ACHUPG_000925"
     CDS             23613..23831
                     /gene="ygdR"
                     /locus_tag="ACHUPG_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR0860636.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23939..24979
                     /locus_tag="ACHUPG_000926"
     CDS             23939..24979
                     /locus_tag="ACHUPG_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR0860637.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25012..26202)
                     /gene="lplT"
                     /locus_tag="ACHUPG_000927"
     CDS             complement(25012..26202)
                     /gene="lplT"
                     /locus_tag="ACHUPG_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR0860638.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(26195..28354)
                     /gene="aas"
                     /locus_tag="ACHUPG_000928"
     CDS             complement(26195..28354)
                     /gene="aas"
                     /locus_tag="ACHUPG_000928"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR0860639.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28940..29971
                     /gene="galR"
                     /locus_tag="ACHUPG_000929"
     CDS             28940..29971
                     /gene="galR"
                     /locus_tag="ACHUPG_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR0860640.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29978..31240)
                     /gene="lysA"
                     /locus_tag="ACHUPG_000930"
     CDS             complement(29978..31240)
                     /gene="lysA"
                     /locus_tag="ACHUPG_000930"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR0860641.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31362..32297
                     /gene="lysR"
                     /locus_tag="ACHUPG_000931"
     CDS             31362..32297
                     /gene="lysR"
                     /locus_tag="ACHUPG_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR0860642.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32284..32976)
                     /gene="ygeA"
                     /locus_tag="ACHUPG_000932"
     CDS             complement(32284..32976)
                     /gene="ygeA"
                     /locus_tag="ACHUPG_000932"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFR0860643.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33105..34523)
                     /gene="araE"
                     /locus_tag="ACHUPG_000933"
     CDS             complement(33105..34523)
                     /gene="araE"
                     /locus_tag="ACHUPG_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR0860644.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8463 a         9504 c         8571 g         8084 t
ORIGIN      
        1 attgaacacc ctaacgggtg tttttttgtt tctgatctcc cataaaaaaa gcgccattca
       61 gcgccttttt atcatcccct tctcgccagc gttgccccat cggcaaaata cgctttaatc
      121 cccgcaagaa tagactccgc aacttcctgc tggaaagtcg ccgttttcag tttacgctct
      181 tcctcaacgt tactgataaa cgccgtttcg accagaatgg agggaatatc tggtgccttt
      241 agtacggcaa acccggcctg ttcaacttga tttttatgca gcttgttgat ttttccgagc
      301 ttattcagta ccgctttacc aaacttaagg ctgtcagcaa tagtcagcga ctgcaccata
      361 tcgaacatgg tgtggtcgac atagcggtca ccgcttttgc tcacgccacc aatcaagtcc
      421 gaggcgttct gggtttgtgc cagatatttt gccgcagtac tggttgcgcc tttggttgag
      481 agcgcaaaca ccgaggaacc gctcggctga cgactggtaa aggcgtcggc atggatagag
      541 acaaacaggt cagcacgctg tttctgggct tttgctacgc gcacttgcaa cggaatgaag
      601 atgtcttcat tgcgcgtcat gtacaccttc atattgccct cttcctcgat cagagagcgc
      661 aaacggcgag ctatttgcaa taccacatct ttttcgcgcg ttttgtattt ccccaccgca
      721 ccggagtctt cgccaccgtg gccagggtca agcataatga caatcggacg atcgcgccct
      781 gctttacccg gttgtggacc actttgcgct ggcggcacct gcttttcgag gtcgcctttg
      841 ttgtaatcct ccagcagcgc cagcagcggg tcctgcatat cctgtgcatt ggccggatag
      901 aggtccatca ccagacgctc tttaaacccg gcgaccggcg caagggcaaa cagctgcggt
      961 tttacgtttt gctttaattc aaaaaccatg cgtacggttt gcgggtcaaa ttgcccgacg
     1021 cgcgccgact tgatgaacgg gtcgtctgca cgaatttgtg ccgccatccc cttgagtacc
     1081 gagttcaggt ttacatcttc gatatccacc accacgcgtt cagggttact caacgcgaac
     1141 tgcttatatt tcagctgacg atttgattct accgtcacgc gggtgtagct ggacgcaggc
     1201 cagacgcgca ccgccacgac ctggctaacc gcagccaggc tgacctgact tacgctcaat
     1261 agccacatag cgcccgcgcc ttgcagtaaa cgacgacggc tgattgcagt gttggatcct
     1321 gacatgcctc tcccgagcaa aagaaatcta agctgtgtaa caagtaaacg actaatttga
     1381 ccggaaactt tagcgaaaga cgcataatct gtcatctaat aaacggtaaa cattcttttt
     1441 atattcacgg cattactgat aaaaaagtcg ctctcgcata aaatttacac ttgcaccctg
     1501 cgaaaaaaca gaataaaaat acaataattt caaataatca tgcaaagggg tgtgccgtgg
     1561 taaaggaacg taaaaccgag ctggtcgagg gattccgcca ttcggttccc tatatcaata
     1621 cccaccgggg aaaaacgttt gtcatcatgc tcggcggtga agccattgag catgagaatt
     1681 tctccagtat cgttaatgat atcgggttgt tgcacagcct cggcatccgt ctggtggtgg
     1741 tctatggcgc acgtccgcag atcgacgcaa atctggctgc gcatcaccac gaaccgctgt
     1801 atcacaagaa tatacgtgtg accgacgcca aaacactgga actggtgaag caggctgcgg
     1861 gaacattgca actggatatt actgctcgcc tgtcaatgag tctcaataac acgccgctgc
     1921 agggcgcgca tatcaacgtc gttagtggca attttattat tgcccagccg ctgggcgtcg
     1981 atgacggcgt ggattactgc catagcgggc gtatccggcg gattgatgaa gacgcgctcc
     2041 atcgtcaact ggaaagcggt gcaatagtgc taatggggcc ggtcgctgtt tcagtcactg
     2101 gcgagagctt taatctgacc tcggaagaga ttgccactca actagccatc aaactgaaag
     2161 ctgaaaagat gattggtttt tgctcttccc agggcgttac taatgacgac ggtgatattg
     2221 tctccgaact tttccctaac gaagcgcaag cacgggtaga agcccaggaa gagaaaggcg
     2281 attacaactc cggtacggtg cgctttttgc gtggcgcagt gaaagcctgc cgcagcggcg
     2341 tgcgccgctg tcatttaatc agttatcagg aagatggcgc gctgttgcaa gagttgttct
     2401 cacgcgacgg tatcggtacg cagattgtga tggaaagcgc cgagcagatt cgtcgcgcaa
     2461 caatcaacga tattggcggt attctggagt tgattcgccc gctggagcag caaggtattc
     2521 tggtgcgccg ttctcgcgag cagctggaga tggaaatcga caaattcacc attattcagc
     2581 gcgataacac gactattgcc tgcgccgcgc tctatccgtt cccggaagag aagattgggg
     2641 aaatggcctg tgtggcagtt cacccggatt accgcagttc atcacggggc gaggttctgc
     2701 tggaacgcat tgccgctcag gcgaagcaga gcggcttaag caaattgttt gtgctgacca
     2761 cgcgcagtat tcactggttc caggaacgtg gatttacccc ggtggatatt gatttactgc
     2821 ccgagagcaa aaagcagttg tacaactacc agcgtaaatc caaagtgttg atggcggatt
     2881 tagggtaagc gatgaaaatc accggatgcg gcaggcgtaa cactcgtgcg tcgcatccgg
     2941 caattacgtt tattcccgcg aactaaacaa cgccgccaga ccactgcgcc gctcagtacg
     3001 agtggcgatt gccgcactta atatacgctc atcggcatac agcgacagac gacgacgcgc
     3061 gcgggtcacc gcggtgtata ccagctctcg cgttactacc ggcgtgcgtt ggctcggtaa
     3121 aatcaacgcc gcatggtcga actccgatcc ctgcgattta tgtaccgtca tcgcccacgt
     3181 agtttcgtgc tctggcaagc gactcggttg cacagactta atattgccgt ccggcatcgc
     3241 aaaccagacg cgcgtcccct gcccgcgatc cagcgcaata ccgatatcgc cattaaacaa
     3301 cccaagcgcg ctgtcattac gggcaatcat caccggtcgg ccttcgtacc aacgagagtg
     3361 cggatgacga tgaattttgc gcttctgttg cataaactgt tcaattcgct cattcagtcc
     3421 agccacgcca aacggccctt cccgcagggc gcacaaaagc tggtactcat tgaacgcctg
     3481 aatgattaaa tccggctcgg cacgcgcttg cagcagatcc agataacgtc cgtaacccgc
     3541 aagagcttcc tcaagcatcg caatataatc ttcgccgctc tgtaaaagtc gtttttcgat
     3601 atcagtaaaa tcctgctgaa aaacggtttt cactgccgtt ttatcaccac ggttgatcgc
     3661 cgcagctaac tgaccaatgc cagaatcgct gccgaaacga tagctttttt gcagcaggca
     3721 gagactgtcg cgcaaagatg ccgcttctgt gccggttcct gccggaacgt gagtccccgt
     3781 caggcggctt agctgcctgg cacgctcggc ggtaaagccc gcgttggcat aagcgcagat
     3841 atcgcccagc acagccccag cctcaaccga ggccagttga tcacgatcgc cgagaaagat
     3901 cactcgcgca tgatctggca aggcgtcgat cagtctcgac atcataggca gatcgatcat
     3961 tgacgcttca tctaccacca gcacatcaag atgcagcggg ttaccggcat gatgacgtaa
     4021 acgctggcta cccggctgcg cgcccagcaa tcggtgcaaa gtgctggcat cttccggaat
     4081 gcgtttcttt tgttcatcgg tcagcggtaa ctgtcgcaaa gccttgccga gagattcggt
     4141 taagcgcgcg gcagctttac ccgttggtgc agccagacgg atacggcagc gttcgccgtc
     4201 ggccatttga attaacgctg ccagcaactt cgccacggtg gtcgttttac cggtgcctgg
     4261 gccgccggaa atcaccgaga tccgccgcgt cagcgccact gccgctgcaa ctttttgcca
     4321 gttaatttca tcgcttactg gaaaaagttt gtccagggtt tgcgccagta gagcttcatc
     4381 aacctcaatg gtatgattca cctcattgaa gaagcgcgct accgtccgct cgttacacca
     4441 catgcgattc aaataaagac gatcgccaca gaggatcatc ggcgtgggtt catctcccct
     4501 gctgaccgct tgagaagcca gcaagcattc ttcccaattt tgtagctcac cgatttcact
     4561 gacacaggtc gccaacagcg gattcgacgc ctcgttattt tccagtcgtg aaagcggcaa
     4621 acagacgtgt ccctctccgg catcatgact taacagtgcc gccgcgaggg tgacggcagg
     4681 atgttcatct cccgccacgg tcagggcaaa ttgcacatcc agcgggcgta gctgtttgtg
     4741 ctccacagct tccagtaatt gcttttgcaa tttcattacg cctcctccag ggtcataccg
     4801 gcaaacatct catccatcag ggcaatcaac ccggcgttgg gtcgggttgt gtaaatccct
     4861 tgttgcggat gttctttatc aacgccacgc aggaacagat agataacgcc gccaaagtga
     4921 agatcatagt cgtaatcagc aatgcgatgg cgcagataac gatgcagcgc cagggtataa
     4981 agctgatatt gcagatcata gcggtgtgcc tgcatcgctg ctgccatagc ctgttgggtg
     5041 taagccgaac tgtcttcacc cagccagttg gatttatagt cgagcaggta ataacgccct
     5101 tcgtggcgga ataccaggtc gataaagcct tttaacatgc cgcgtacctg catgaactcc
     5161 agcggcgggc agccagcgga tagcgggtca aactggcgaa ttagtgcatc aagctggctg
     5221 gcgatgagcg gttcgctaat cggcaggtaa aactccatct ccacctgttt atcgcggtcg
     5281 gaaagctgac tcaggctaac acccgtttca ttgagaggtg cctggaggac agccgtgatc
     5341 cactcggtca aaaccggttc ccactgcgat tcaaagccgc cgagttccag tttttcctgt
     5401 acccagttcg ggtcaaccgg ctgggtaaaa tcgaggtctt caaacaaact gtgcaagaat
     5461 gtccccggtg acgcaccgcg cgggaactga tgcggtgtta acgtcggttc ttcaacgacg
     5521 ctgaccacgc ctgcggcatc aacatccagc cgtggcatca gatcctgagc gataccgtga
     5581 ccacgctgct gcaagccgga gtagctggtg acacgccagt tatcgccggg caatcgttgt
     5641 agtgtcctcg cattcagttc tgcagttaaa gcatcattaa cctgccaggg ctggttatca
     5701 ccagtttgtg ccgtttgcca ggcaatatca tcatcgcata acgcttcaat acaggtgcga
     5761 agccctgccg catcttgcgg ttcccctttt tgcagcaaac gcccgagcgc actttggtgg
     5821 acgtcggtgt cacctttttt atcgccacga cggcgcacca gcggtgcaac gccgagactg
     5881 caatgccaaa ccgaacgcgt cagcgccacg taaagcaaac gcagatcttc cgccagacgt
     5941 tcgacctccg cgaggtcgac gctttctggc gcagcattaa gatccaaaac tgcctcaaac
     6001 gagtggcgat cgtgataaaa cgcctgatcc tggacgcgga aattggtaat aaatggcaac
     6061 cagaccagcg gatattccag ccctttcgat ttatggatcg tgacaatctg caccagatgt
     6121 ttatcacttt cgaggcgcat ttgttggctg gaggcattac tgtctggctc gaggatatgt
     6181 tgcgataacc agcgtaccag cgcatgttca ctttccagct gcgttccggc ttcttgaagc
     6241 agttcgctga tatgcaagat atcggtaaga cgccgctcac cgcctgccgt tgccagcaag
     6301 ttttctgcaa tgttacgcgc cgacatcagc gcccgcagca tcggcataac gccacgtttg
     6361 cgccagattt gccgataacc atcgaactct tcgactaccg catcccacgc atgttcgtca
     6421 ttattcagcg tttcaatatc cagcgcattc agccccatca ttgacgttgc cagcgcactg
     6481 cgcagggtgt tctcacgttc gggcgtcatc accgcctgca acaaccaaag catttcctgc
     6541 gcttccagag tttcaaaaac actgtcgcgg ttcgaaaggt aaacggaagg gatttccagc
     6601 aacgttaagg catcgcgcac ctgggcggcc tcctggcggc tgcgcaccag cacgctgatg
     6661 tccgaagcac gcaccggacg cgcgtcgtcg ccgttcatca gcaacgcttc gccccgctgt
     6721 ccggcttgta gccagtcgcg gatttgcgca gcacatacct gcgccatggt actttgatag
     6781 tcgccaacgc cgcagctttc gccttccatc agccacattt tcatcgcagg ctgcgtttca
     6841 cctttaaata caaaacgtaa cgcctgattt ttcccggctg atttcactgg aataaacggt
     6901 atttcgcgaa acatgaacgt gtcatcagtc tggctgaaaa gcttgttcac actgttcacc
     6961 attccaggtg cggagcgcca gttggtgtct aaagtgtagt gggcatgaac ttcactacgc
     7021 gccttcatat aagtgaagat atccgcaccc cggaatgcat atatggcctg cttcgggtcg
     7081 ccaatcagca acaatgcggt ttccggctga tggtgccaga tacggcgaaa aattcggtac
     7141 tgctgggggt cggtatcctg aaattcatcg atcattgcca ccgggaatcg cgtacggatc
     7201 gccgctgcca acacctcgcc gctttcgcta cgcagcgcgg aatcgagccg acttaacatg
     7261 tcatcaaaac ccaattcgcc acggcggcgt ttttcacgcg ctactgtttc gcggatctca
     7321 gccaatgcgc gggtgatcaa cagatcgcgg atcgacaatg gttctgcaag cagttgatcg
     7381 atcgcctcaa acagtggatg tcgcggggtt tcccccccgg ccttcgtgcg atcttctaag
     7441 aaacgctggg agaatttttc cagcgactcc ggcaactgat aactgtttgt ctcttcttct
     7501 gcccaggcgc tgatcttttc gatccattta gcctgattgc tacggttaaa cttgcgtcga
     7561 tcaataccag aagattcgat cagcgcatcc agttcaccca ctgcgtcgcg ccactgctgt
     7621 tttaccgtat caatacgcgc cacaatttgc gcgtggcggg aagccagcgt ttcatcatcg
     7681 ggcggcggtg ctttgataac cggcggttcg ccttgcagat aacgattaat atcgcgcagc
     7741 aacgcctgcg gccctttcca ggtttcaaag acgacctggg ctatttcacg cggcagcggg
     7801 tagcagtggc gacgccagaa atcggcgcag gcctggtagc gtagcagaga ctcatcttca
     7861 atcagctgct gctcaaacag catgccggat tcaaaggcat tcaggttgag catgcgctgg
     7921 caaaagccgt gaatagtaaa gactgccgct tcatccatct gccgttcggc taacaacaac
     7981 cactgcgcgg cttgcgcttt atcgtcgatc tcttccagca ggcgttcgta cagtggattg
     8041 tcggtggttt cacgcagaca ggcgatgcgc aactcgtgga tattgctacg gatacgaccg
     8101 cgcaattctg ccgtggcagc ctcggtaaag gtgaccacca gcagttcttc aacgatcagc
     8161 gggcggggaa aggcggcgga accgcctagt ccaagtaaca ggcgcaaata gagcgccgca
     8221 atcgtaaagg ttttgcctgt gccggcagag gcttcaatca ggcgctcgcc ctgtaagggc
     8281 aagcgcaaag gatctagtgt ctcggcgaca tcactcattc ttttcactca tcaggggcat
     8341 tgtttgctgc aacgcgctga cgttctccca cactttccag ccttcaggat gcacatattc
     8401 ggctttcccg ttctggctgc cggaaatctg cgacagaata gccatgcctt gcggctcgac
     8461 caccgcctga tggaagaaat cagcaagttt ttgcggcgtc agcagtttta tctgggccac
     8521 gattttatca cgcgaatcga agcgcatatt gccgcgatcg aaatctttac ttaacttcga
     8581 tgcttcttcg ccgagcgttt gcggtgcctg cagcatctgg gtaattaccg cctgctggat
     8641 ttgcgcaaac tcatctggct tcatcgctcg caattttgcc tctgcggttg ggaaaaacgc
     8701 cttgtaacgc tcccacaaga atgaaggctg tttatcattg ctttgcaaaa ggaagcccat
     8761 gccccactga cgccccacgc tcattggaaa cgcaaacacg gcatagccca attgttcttc
     8821 ggtacgcaac tgattgtaga accacggctg tacgatctgc cccaacagag agctataggc
     8881 tgagctggtg tattcatcgt agccagtcgg tacaaatacc gctgccagtg cggagtcggt
     8941 gctgttaccg gctttttcaa agatgacgga ttgtttttta tcgaccacta catctttgtt
     9001 tcgacaccac tctgaaccat cagcgcccaa ctgtttttgc acatcgcgtg ccagcgttgt
     9061 tgcctgggcc tcggtcatgt tgccgataac cataaactct ggtcgagccc ctgattttaa
     9121 ggcgtcgcga taggccagca cctctttcaa cgtaatggag ggcaaaattt tacgccgttc
     9181 atctcgcgag aagtacggca cttgcgagag catctgcgcg ggcataatcg cctgctcaaa
     9241 cgctttaccc ttttctgcgg aatccatcat ctggttatac caggacttcg cctgctcaag
     9301 ctgctcttcc gtagcggtat agctaaagta cccctcgagc aatgcctgga acagctgcgg
     9361 cagacgctgg gtgtaaccat tagcattaat cataaggccg ttgttagcgt tggtggaaaa
     9421 acttatgcca ccaaccgacg cctggttgct taactgatca agcgccagcc ctgcgagata
     9481 atcattgagc gcaaacatca cctgattgcg ggcgctgtcc atggctttcg gattacgcaa
     9541 aatcaggctg acatcagctt tgggctcgct ggcaaaataa cggcttggcg catacaccac
     9601 gcgcagattc gactcatcaa caatcagctc tggatggtcg tatttcttct ctgacttaat
     9661 cagcgagaaa tcatcaggaa tataagggtt aagctctggc aaagagagcg caatgtcggc
     9721 ggcttttttc tgccagtcgg cgaaagtttg tgcgctgatt ttatcgacct gatacggcgc
     9781 atcgacaaag taagccgttt tgttgtgcgg ctctttcggg ctgatatacc agatacgcgc
     9841 attctgcggc gtcatcatcg ccagacgttc ctttactgct ttagcatcgt accgatcggc
     9901 aatattgact gcatccagcg tatgctcaac aggaacgcga atcatggtat ctgccagcca
     9961 ttcgacgtaa tccatatcac gggtgatcga cggataacgg aagtcgatat ccagcacatt
    10021 cgccagttca tcgaagtatt gtttatcaat gcctttttca cgtaacagat tgagatagct
    10081 aaaaattgcc gccacaacct gatcgcgatt agccaggcct ttatcagtta aagacgcaga
    10141 gatcgctaat acgccgctgt tgccgttgac gataggatcg gagttggcgc taatgccctc
    10201 aactaatccc tgcttttgca gccagtcaga aagtgtacct gggctgcgat tgccaatcag
    10261 ataggtaatc aattcatcgg ttttactacg gaacttcgct gagttgttat cgatgcgaaa
    10321 ctcaacgcgc aacactttac gcggcagcgc agggacgtaa tgaatgataa tgcccttttg
    10381 cgcgtcggtg actaccggca cggtgatttc cggttttttg ctctctttgt tcggcacgcg
    10441 accaaaggtg tccgccgcca tttttgccaa ctccggcagc ggtttattac tgtaaataac
    10501 cgccttcatc aaattggcgg aatagtactt ctcgtggaaa tctttcagcg cctgctgcac
    10561 cggattacca ggtttgtcgc ttaaagtttc gaggttacca ccagaaaact ttgaaccggg
    10621 gtgtgccggg ttaatggttt ctgcgctgac ctgtgccatg cgcatcccgt cacgcgtacg
    10681 cgccatggtt aattcagcgt tcactgcatt acgctcacgt tcggcgtatt tcttgtcgag
    10741 caagggttct gcaatagcat cggccaggcg gtctaccgca ccgggcaagg cgtcgttctc
    10801 aacttccaga tagaaagccg tgcgatacgg cgcagtgctg gcattgtgac taccgccgtg
    10861 cattttgaga tattcggcca gactgtcagc ctgcgggtac tttttcgacc ccatcagact
    10921 catatgttca aggtaatgtg ccagcccctg gtacgcctcg ggatcttcca gcgacccaac
    10981 gggcactacc agcgccgaga gcgatttaac tgcctgcgga tcagaaacca gcaagaccac
    11041 cataccgtta tccagacgta tagcctgata ctggcggtta tctttatcac ttttacggat
    11101 ggtttcctga atcggctgcc atcccgtttc tgcctgactt aatggtgccc agagggcaac
    11161 aaacaacaat aatgctttga accaggtgct gcggggcatt cacggacctc ataatcaact
    11221 ttattttctg gacagattca acaggttaac gcaaatcatc tgccagaatt taatcttgtg
    11281 ctgcacgagt cagcctatgt ttatataacc atcagtccgt gactggtgcg catcataaag
    11341 taagcagata gattgcgcaa tttttataca acactcatga ctgattaaag cgaaacagcg
    11401 gtaacaggaa acgttgcgac tgttcaacga tggcctccat tgtctctggt gttaattgcc
    11461 gccagagccg ttgataccag atatcatcac cttcgccacg caccatcatg ttgccttcgt
    11521 aagcctgaag gaatttcgta cgggcttttt gcaacgtgga atcgtcatcc agcatggcat
    11581 cgttttgcgc gtcataacag gtttttagcc acgcgccgcc actttcaggt aacaccagca
    11641 atggcgcgga cattccttca cgatacccct caatcagttg tgagaggtaa tgcaaagcct
    11701 gttcggctgc aagcggcgga aaacgccact caccgtcttt gcgtagaaaa aggcgacttt
    11761 cgccattacc accgctggca cagtagacaa ggtgttccag ccaaagttgc attccctgcg
    11821 ccacacttaa taaagaggga cgccagcgca acaggccatc cggctgcacc tgcggcaacc
    11881 agccagttat ctgcacaccg ttgcaggcga gatcaatttc catactctgc cccggctggc
    11941 gacaggcaat gactctgtcg gcaagctgct gcatctcctg gcactgtgtt tcccagaaaa
    12001 tttcaccaaa agcgccatac ggtaaatccc ctgccgcccg gaagcggcgg aacaagcgtt
    12061 cggcatcatc ctgctcaacc agtgcattca ataactgctg attgatttga taacggctaa
    12121 gtccttccag aataaatggc tcggtgtctg ggatttcgct gtcttcagta tggaagttca
    12181 cctgcaaacg catctggaag aacgcccgca ccggatgtgc ccagaatcgt tgtagcgttt
    12241 ccagcggcac ggtttccggt aaggtaaacg gcagcggctg aacaaattca gaatgtgctt
    12301 taccagcctg gctggccgca ggtagccatt cacgagcata gctttgtcgt tcgcctggct
    12361 ggtagttttg cggatcaaac ggcatccggg tatggaggca agtaagatgc gcttttaccc
    12421 ttgcctcgct ttcatcacag ttgagcgctt catcgcccgg tagataatga ctttgcccga
    12481 tgtagtcgat cagttcctgc accagtaccg acgggaaacg ttcactgtta tcctgaatgg
    12541 aacgcccgat atagctgata tagagttttt gctgcgcgga aattaacgct tccaggaaca
    12601 ggtagcggtc gtcatcgcga cggctacggt cgccacgctt cggtttctgg ctcatcaggt
    12661 caaagcccaa tggcgcaagc tgacgtggat aaacgccgtc gttcattccc agcaggcaaa
    12721 ccactttgaa cggaattgaa cgcattggca tcagagtaca aatgttaacc ggtccggcga
    12781 gaaaacgctg gctgatacgt tcttgatcca gacgctgtgc cagttcatca cgcaatagtg
    12841 acagcggcac cgcgtcgcca tactgcgcac ctaaaccttc ggcgataatc gcctgccatt
    12901 gttgttcgat cagcgtcatc gccgcttcgg tttccgcatc cggcaggaag aaggcgttga
    12961 gcatatcgcg acaaaccggc aaccactctt ctagcggacg ctcctgtgcc agcccgcgac
    13021 gccagatgtt tagctgcatt agcagtgaag ccagatgccc caccagttct gcaattaagc
    13081 cgctcgattc atcataaggt agaaccgatt gccactcgcc ctgcgcgctc tccatcgcgt
    13141 agcccagcaa catacgcgtc aggccaaatc gccaggtgtg ttgtccggtg gcggggagtt
    13201 ccagctcgcg aacgttgtcg tcatctatcc cccaacgaat gccggattcg ttgacccact
    13261 ggcgtaaata acgcagccct tcttcggtga tgtcaaaccg cgccgccagc accggcacat
    13321 ccagcagcgc cagcacatcc tctgacacaa agcgactgtc aggcagtgat aacaggctga
    13381 taaacgcttc cagtaccgga tgtgactgcc gcgcacgacg gtcggaaatg gcgtaaggca
    13441 ggtaacgatc cgcaggtgcg ctaccaaaca ctgcctgaat aaacggactg tagctgtcga
    13501 tatcagccac catcacgatg atgtcgcgcg gagtaagtgt cggggcttcc tccagcatcg
    13561 ccagcaagcg atcgtgtaaa acttcaactt cacgctgcgg gctatggcaa acgtggaatg
    13621 tgatactgct atccagtgga tcaagcgggc gtttgttatc gctacgggaa aactcttcga
    13681 tgttcacacc cgcaacggcg cggttttcca gttccagaat gtcagactga atgttatgca
    13741 gcaggttatc tggcgtcaca tcgacaaaag cgtccagctc ctggctgctc tccaggtcag
    13801 aaaggagata aatgtagtcg cgcccaagct tcccccatga ggccagcagc gagttgccga
    13861 catcctgttc accatcgctg ttaaagagct gtccggcatt ttcgctatcg cgaaatagcg
    13921 gcaattcgcg atcttcaaaa ctgtgtcgac gctgacgagt cagcagtttc gccagataag
    13981 caggatcttt aatatcgccc cagtaataac ggcaggggtt ggtaaacagg agatggattt
    14041 caatatgttt acccagcgcc tggagcgcct ggagataaac aggcggtaac gcggaaatac
    14101 cgcatataaa gacgcgcgaa ggtaaccccg gcgggcaggt cgtcgcggac tccagcgttt
    14161 cgataaagcg ctgatagaga ttcgcgcggt gccagcgcgg ttgtccgagt tcatgggtat
    14221 attccaccag cgccttccac aacggggctt gccaggcctg tgcttctccc agcccttcaa
    14281 ccaaatgtcc tgtttcccac tgtgccagcc agtccggacg atagaccaga tactggtcaa
    14341 acaggtccgc cgcttttgag gaaagctgga acagttttcg cttgtcgcta tcgtcagtca
    14401 gataatgccg caacagggta aagtcttcgc gctccagcaa ttgcggcagc agagtcatca
    14461 gtttccagct catgctctgt ttgttaaagg cgctctcttt ggggatttcc ggtaacaccc
    14521 ggacgaacat atcccagata aagctcgctg gcagcggaaa atcaatgttt gccgcaatac
    14581 caaacttttg cgacagggtc atttgcagcc actgcgccat accggtactt tgcaccagaa
    14641 tcatctctgg ttcgaaagga tcgtccagcc gttcgcgttc gacaataaac tccatcaacg
    14701 cttccagcac gtccagacga ttggaatggt agacccttaa catagcggct cctgactact
    14761 gacaattcgg gcaatgcagg cgggtcatct cgccttctct gcccccgggt gaaactagcg
    14821 taacgctgat gctgacacat cccgcctgcg atgtctgcat tcggttgacc tgccagttgg
    14881 caggtggcga aatcgcgcgc agttgcgttt gctgccaacc atgccgccag agcttttggt
    14941 actggtttct gctggcgaga ctgttcattg atgttcgctg gatacccgat aacgcagtga
    15001 caatcatcac catcagcacc atcagcacca tcagcaccat cagcaccatc gcaaccatta
    15061 cctccggcag gctaaagcct tgttgattct tcagggaagc tgacataatg ccccctcttt
    15121 cagcggacaa aaatcgctcc agccgcgtgg cgaaaaaaca atttttccat caatgacttc
    15181 gcctgttcgc cacaacgaaa cgccttcata accggcaatc aataaggctt cattatctgc
    15241 cagtaaacgc aaacaaacct gggcatcggt tccagcgtac tgcgagcact gaactgctgg
    15301 ctgcgtctgc caggagtgca tttttcccca cgccagcgcc gactgaacga tggcctggcg
    15361 gcgcaatgac tgactttcca tgctcacgcg agaggcaaaa ctgcgatcct gctgactcat
    15421 tccttgcaat agcaagctac ccaaaaccag cagcatcagg accagagcca gtgacgaaac
    15481 acctttttcg cggttcacag gttgaatcct gtcacgctat agctggcatc caccacggtt
    15541 tgcggttcag acttactggc agcacgcata ttaaccgtca acaccggcga gaagccgctg
    15601 acatcctgac gtacgacctg aaaagtgtcg ataatgatgg catccggatt agtgacttta
    15661 tcccagccct taccttcaca ggatgtcgca ccgcgtagcg tttccagcac atgctccttc
    15721 agacgaaatc caatctggtc ggactctttt accggttcgc gatcccagat accgttactg
    15781 ttcgcatccc actgcacaat gatacagtca ccctgtccga caatttccag cccttcgccg
    15841 gtacagctgc catgacaata acccgccctc tggagatgct tcgcgacggt aaatacccgc
    15901 agccagattt catcttccag tgccagctta cgagtgctcg ttaaactttc acgctgtaac
    15961 gcaggcagaa agcgtgcagc ccccagcaac aatacgctac tgatcgccat agcaatcaac
    16021 acttccagca gagaaaaacc ttgctctttt acaggcatcc ttctgtttct ccttgctgat
    16081 aaagccggag tcttccccac ggcgaaacca ccagccacca ctcgcccgtt gagtttttga
    16141 agcgaatatg cacggcccat gcggtattgc gcaggccaaa gaaagcaagc gaaggtgtca
    16201 ggtcgctcat ttcgacttcg ggccagcgtg gcacaaagac caatggtgaa ctgccatgac
    16261 aggtattggc cccagcagcg gaactcacaa ggcaccataa cgtcccctcc ctgataacgc
    16321 tgatactgtg gtcgcggtta tgccagttgg catcttcacg taaatagagc aaatagtccc
    16381 gcgcctagct ggcggtttgc caaagccgtt gcgactgctg ccagtattgc cagccataga
    16441 gtccacttgc gcttagcatg accaaaatca gcatcgcgac cagcgtttca atcagcgtat
    16501 aaccacgttg tgttttcatg ccggcagtat ggagcgagga gaaaaaaaga cgagggccag
    16561 tttctatttc ttcggcgcat cttccggact atttacgccg ttgcaggacg ttgcaaaatt
    16621 tcgggaaggc gtctcgaaga atttaacgga gggtaaaaaa accgacgcac actggcgtcg
    16681 gctctggcag gatgtttcgt aattagatag ccaccggcgc tttaacgccc ggatgtggat
    16741 cgtagccttc aatctcaaag tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt
    16801 tgataatcaa cttcggcagc ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca
    16861 tatggttgct gtacaggtgc gtatcgccac cggtccagac aaaatcaccc acttccagat
    16921 cgcactgctg cgccatcata tgcaccagta acgcgtagct ggcaatgttg aacggcaggc
    16981 cgaggaagac gtcacaggag cgctgataaa gctggcaaga gagtttgccg tctgccacat
    17041 agaactggaa gaatgcatgg cacggtgcca gcgccatttt atccagttcg cctacgttcc
    17101 acgctgaaac aataatgcgg cgcgaatccg ggtcgttttt cagctggttc agtaccgtag
    17161 taatctggtc aatatgacga ccatctggcg ttggccaggc gcgccactgt ttaccataca
    17221 ctggcccgag gtcgccattt tcatcggccc attcgtccca gatggtgaca ttgttttcgt
    17281 gtagataagc aatgttagta tcgccctgca ggaaccacag cagttcgtgg atgatggaac
    17341 gcaggtggca acgtttggtt gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct
    17401 gatgaccaaa aatggaaagc gttccggttc cggtacggtc gtttttctgt gtgccttcgt
    17461 cgagcacttt ttgcatcagt tctaaatact gtttcatggt tcctcaggaa acgtgttgct
    17521 gtgggctgcg acgatatgcc cagaccatca tgatcacacc cgcgacaatc atcgggatgg
    17581 aaagaatttg ccccatgctg atgtactgca cccaggcacc ggtaaactgc gcgtcgggct
    17641 ggcggaaaaa ctcaacaatg atgcgaaacg cgccgtagcc aatcaggaac aagcctgaga
    17701 cagctcccat tgggcgcggt ttacgaatat acaggttgag gataataaac agcaccacac
    17761 cttccagcag cagttcgtaa agctgtgatg ggtggcgcgg cagcacaccg taagtgtcga
    17821 aaatggattg ccactgcggg ttggtttgca gcagcaaaat atcttctgta cgggagcctg
    17881 ggaacagcat ggcaaacggg aagttcgggt caacgcggcc ccacaattca ccgttaataa
    17941 agttgcccag acgcccggca ccaagaccaa acggaatgag tggtgcgata aaatcagaga
    18001 cctggaagaa ggaacgttta gtacggcggg cgaagataat catcaccacg ataacgccaa
    18061 tcaggccgcc gtggaaagac atgccgccgt cccagacacg gaacagatac agcggatcgg
    18121 ccataaactg cgggaaattg tagaacagaa cataaccaat acgtcccccg aggaagacgc
    18181 cgaggaagcc cgcatagagt aagttttcaa cttcattttt ggtccagcca ctgcccggac
    18241 gattcgcccg acgtgttgcc agccacattg caaaaatgaa ccccaccaga tacatcaggc
    18301 cgtaccagtg aagcgccacg ggtcctattg agaaaatgac cggatcaaac tccggaaaat
    18361 gcagatagct actggtcatc tgtcaccaca agttcttgtt atttcgctga aagagaacag
    18421 cgattgaaat gcgcgccgca ggtttcaggc gctccaaagg tgcgaataat agcacaaggg
    18481 gacctggctg gttgccggat accgttaaaa gatatgtata tgatccgcgc tataaccctc
    18541 cgcgaatcag cccgcccatg ccgcgacgct ccataaaggc tgcaacctga tgacgaactt
    18601 cggtcgccag ttgcgcttcc agactacgct gcgcaagatt ttctgcttcg gcaaaatcaa
    18661 tgcgccgcag caggtatttt acccgcgcta cagaacgtcc gttcatagac agatggcgat
    18721 acccaagccc aatgaggatt gccacgcaca tgggatcgcc cgccatttca ccgcacaaac
    18781 gaagatcgat accatgtatt tccgcttccc gggcgatcat cgccagagct cgtaacattg
    18841 caggatgaag actgtcgtaa atgttcgcca cccgggtatt gttgcgatca acggccagaa
    18901 tgtattgagt cagatcgttg gtgccaacag agatgaaatc gaccctcttt gccagatgcg
    18961 gcagcataaa taccattgac ggcacttcca gcatgatgcc gatacgtggt ttgggaattt
    19021 cgtaaccgat catctcctcg acttcacgtc cggcacgttc aatcaggcgg cgtgcttcgt
    19081 caacttcatc gaggctggtg accatcggca acagaatatt caggttgccc gtagcggcat
    19141 tagcacgcag catcgcccgc acctggatca agaagatctc cggctgatcg agcgtaatgc
    19201 gaatcccacg ccaacccagg catggattct cttcgctgat cggcatgtaa ggcagctgct
    19261 tatctgctcc gacatccagc gtacgcaagg tgacgggttt atcattgaac atttgcagca
    19321 tcccctgata ctgcgccacc tgttcttctt ccgacgggaa accactttgc agcatgaatg
    19381 ggatttcagt gcgataaaga ccgatgccat caatacagct gcccagtttt tcttcatgtt
    19441 ccgggcttaa accagcattg agcatgactt ttatacgctc accgcttttt aactgcgcgg
    19501 gtaaattgac gtcatcttcc gccagacggc taagctcaat ctcttcacta attagccgct
    19561 gatattcttg cagcagtacc ggctccggat cgaccagcaa ttcaccgcga tagccatcaa
    19621 cgatcagcgt cctacgatgc agcaccgaag gctgaatatc cgcgcccatc acggtaggga
    19681 tccccagcgc acgtaccatg atcgcagcat gggagttggc tgcgccatct cgcacgacaa
    19741 caccgactaa gcgatcctgg ggcagctcag caagagttgt cgctgacagt tcatctgcca
    19801 ccagaatgaa acgttccggc caggcgttcg gcccttgatt agcgtcatca agatgaaaca
    19861 gcaatcgctg acccagcgca cgtaaatcac cagcccgctc tttgagatag ttatcgctta
    19921 gcgcggcaaa ctgttcggca aatttttcaa tgaccgtttt taccgcccac tctgccaccg
    19981 agcctttatc aacctcggca aacaattcgc gacgcagccg ggtgtccgaa agcaggtgcg
    20041 agtaaagatc gaaaatagcc gccgtttctt tttgtgctcc ggcggcaaag cgtttgctgt
    20101 agcggcgaaa ctcgtttgcc gcttcttcca gcgccccggt cagtcgttcg cgttccagag
    20161 ccggatccag cgttgatgcc tgatacacct gttccattaa aggtaacgtg gcatcctgcc
    20221 agccttcggc aatcgccaca ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat
    20281 attgcccaaa cagggcagtc aactgcgact gagaaagaat agctgccatc tgggtggcaa
    20341 gcgtcaccag gaaggattct tcactttcgt catactggcg caactctcgt tgctgtacca
    20401 ccagtacacc aagcaactgg cgacgttgaa taattggtac gcctaaaaac gcgcggaaac
    20461 gttcttcttt tacggagggg atgtatttga agctggggtg cttttgcgca tctgcaaggt
    20521 ttatcggttc cgccagcctg ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta
    20581 cagtgcgacc gcgtggtttt ttcagccccc gggtcgccat caggtagtaa caacgtcgat
    20641 catgatcggc caggtagacc gaacagacct cggtatccat cgcaagacag atgtcggtaa
    20701 ccagaatatt taacgcctca ttcaggcgtg gtgcgctggc taccttttcg actatttcgc
    20761 gcaggcgagt gagcataatt ggcgtgactt aacctctttt acgtcgataa gcagatgcgt
    20821 tttgtggttt tggcgtattt tcctgcagtg acatcaccac actcgcgaac tctttcatta
    20881 ccctacggta gacatcacgt ttaaatgaca ccacctgtct gaccggatac cagtaactta
    20941 cccatcgcca gccgtcaaac tctggtgtac tgctggtttg catattgatt tctgcatcgc
    21001 cgcttaccag ctgcaagaga aaccattttt gtttttggcc gatacaaacc ggcttcgtgt
    21061 cccaacgcac caaacgtttc ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga
    21121 ttcgaacgtc tttgcggctt aatcctactt cttcaaacaa ttcacggtac atcgcctgct
    21181 ctgcggattc tccggggttg attccgcctt gcggaaattg ccaggagtgc tgaccaaatc
    21241 gccgggccca cattacctgc ccctggcgat tacaaatcac gatacctacg tttgggcggt
    21301 agccatcgtc atcaatcacc ggactacctc aaaataaagc tttatatacg aatgattgtt
    21361 tcatactcca ggaagacggt aaaccactct ctgcagggca ttacacacta ataacaattg
    21421 aataactcac agttatgtgc agagttataa acagaggaag gggtggatag ccgtttttat
    21481 tcaccttttc tgtggataga gttgtgaaga actacggaat tactacggga aaacccggag
    21541 cattctgaat aagcccgata tagacatttt aaaatattca ttattagtca atgcattaca
    21601 tgtaatttga gtaatgaaaa agagtaattc gtgacccagg tcacacctct catttacggg
    21661 ttggcgaaag atcaaccaat gccgtgttta tccacagaat gtgccactaa gttaagcact
    21721 gaaccactaa aaactggagt ttcgtcgcac gtcaaggctg taaatgaaaa cagtagtgga
    21781 ggtttttcac agttatccca gctttctgtg gataacatgg tgtaagatcc tgtttatttt
    21841 cagtgaccag atttggaaaa cctattgcag tgttgcgcaa ctcgattacc ggcagcctaa
    21901 aaagcaatat aaatcagaga attgaacaac acatgtggaa aaagttacac tgcgaatatt
    21961 cggcacataa ttgctgtttg ttttttaacc aaggtatcat gacatgtccc aacctcgccc
    22021 actgctctct cctcccgaaa ctgaagaaca gttgttagcg caagcacagc aactttctgg
    22081 ttatacattg ggagaactgg cggcacttga cgggctggtt acgccagaga atttaaaacg
    22141 cgataagggc tggattggcg tgttactgga gatctggcta ggtgccagcg cagggagtaa
    22201 acctgagcaa gattttgctg ctctgggcgt ggaacttaaa actatccctg tggatagtct
    22261 tggtcgtccg ctggaaacaa cattcgtttg tgttgcccgg ttaacgggta atagcggggt
    22321 gacctgggaa accagccatg tgcgccacaa gctcaaacgc gtactgtgga taccggttga
    22381 aggcgagcgc agcatcccgc tggcgcagcg tcgcgtagga tcaccgttgc tgtggagccc
    22441 gaatgaagag gaagaccgac agctacgcga agactgggaa gaattaatgg atatgattgt
    22501 tctcggtcag gttgagcgga tcaccgctcg tcacggggaa tatttacaga taagaccgaa
    22561 agcagcgaat gcgaaagcgc ttaccgaagc cattggtgcc cggggcgaac ggattctgac
    22621 gctgccacgc ggcttttatt tgaagaagaa tttcaccagt gcactactgg cccgtcattt
    22681 tctgatccag tagccatcgc tttgacctgc cgctttccgg gcatataatt accgcttcat
    22741 ttttttggca gggcttttta gatgttattt gcatggataa ccgatcctaa cgcttggctt
    22801 gcgctcggta cgctgacgct gctggagatc gttcttggga tcgacaatat tattttcctt
    22861 tctctggtgg tggcaaagct tcccacagca caacgtgctc atgcgcgccg tctggggttg
    22921 gcgggagcca tggttatgcg tctggcgctg ctggcatcaa tctcttgggt tacgcgcctg
    22981 acaaatccgc tttttacaat attcagtcag gaaatttctg cccgtgattt gattctgctt
    23041 ctgggtggct tgttccttat ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt
    23101 gaagaagaag ggctgaaaac acgcgtttca tcattcctcg gcgctatcgt gcagattatg
    23161 ctgctggata ttatcttcag cctcgactcg gtgattaccg ctgtgggtct gtcagatcac
    23221 ctgtttatta tgatggcagc cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc
    23281 tcgattggtg attttgtcga acgccatcct tcggtaaaaa tgctggcgct ctctttcctg
    23341 attctggtgg gctttaccct gattctggaa agtttcgaca tccacgtacc gaaaggttac
    23401 atctacttcg cgatgttctt ctctattgcg gttgaaagcc tcaacttgat tcgcaacaaa
    23461 aagaatccgc tctgatactc cgtacgctct cctgcgggag ggcgtactca ctccttttac
    23521 cctcacagtt taagattttc ctgctttcaa gatatataac gtcggtttat aaacagacta
    23581 ttatcatagg taggccaaat gaggataacc ggatgaaaaa atgggcagta ataatttctg
    23641 cagtcggact ggcgtttgct gtttccgggt gttccagtga ttacgtcatg gcgaccaaag
    23701 atggccgtat gattttgacc gatggaaaac ctgaaattga tgatgatacc gggctggtga
    23761 gttatcacga tcagcaaggt aacgcgatgc aaattaaccg tgatgatgtt tcgcaaatta
    23821 ttgaacgtta acaaataagg tcagcatccg gctggcctta agatttttct ctttcccttt
    23881 tcccttccct ctgccatttt tatattcctt atgtcgtgat tataaaaagg aaacggctat
    23941 gcaatatcac cgtatacccc acagttcgct ggaagtcagc acgctggggc ttggcacgat
    24001 gacgtttggt gaacagaaca gcgaagccga cgcccacgca caactcgact atgccgtcgc
    24061 tcagggcatt aaccttatcg acgttgccga aatgtaccca gtacctccgc gccccgaaac
    24121 gcaagggtta accgaaacct acgtcggcaa ctggctggcg aaacatggca gccgcgaaaa
    24181 gttaattatc gcctccaaag tgagcggacc gtcgcgcaat aatgacaagg gcatccgccc
    24241 ggatcaggcg ctggatcgga agaatatccg cgaagcgctg catgacagcc tcaagcgcct
    24301 acagactgat tacctcgatc tttatcaggt gcactggccg cagcgcccga ccaactgctt
    24361 cggcaaactc ggttatagct ggacggattc tgcgcctgcg gtttcgctgc tggatacgct
    24421 ggacgcactg gcagagtacc aacgcgcggg aaaaattcgt tatatcggcg tgtcgaacga
    24481 aactgcattt ggcgtaatgc gctacctgca tctggcggac aaacacgatc tgccgcgtat
    24541 tgtcaccatt cagaaccctt acagtctgtt aaaccgcagt tttgaagtag gtctggcaga
    24601 agtcagccag tatgaagggg tcgaactgct ggcctattcg tgcctgggtt tcggcacgct
    24661 gaccgggaaa tatctcaatg gtgcaaaacc cgctggcgca cgtaatacgc tctttagtcg
    24721 gttcacccgc tatagcggtg agcaaacgca aaaagccgtc gcggcgtatg ttgatatcgc
    24781 cagacgtcat ggccttgatc ctgctcagat ggcgctcgcg tttgtacgcc gtcaaccgtt
    24841 tgttgccagc actctgctgg gcgcaaccac gatggatcag ctgaaaacta acatcgaaag
    24901 tttgcatctg gagttaagcg aagacgtatt agctgaaatt gaagcggtac atcaggttta
    24961 tacttatcct gcaccataaa gattaacgcc ggttaaaacc ggcgttaaat attaatgacg
    25021 gcgctgccag atccacagcg ccgttattgc cagcgcaaat agcgcaccga agccaatgcc
    25081 aatgggcacg accgggatgc ctaccattac cgccagcgag taaatgccca gcatcaacaa
    25141 catggcgctg ttttcgccaa ggttttgtac tgcaatcgca ttccccgccc cgacgctttt
    25201 tttaccccgc tcctgtagca acgcattgag cggaacgaca aaaaagcccc ccagcacgcc
    25261 aatcagcatc aacaaggcat aggctggcag cagctcgtgt tgcagggaga aaatcggtac
    25321 caccacgcca atcaaaatcc cggctggcat acagcgtgac acggtttcca gcgtaaccag
    25381 cttcgctgcc gcacctgcgc caaccacgat accaatcgct accatcgcgt tgagataggt
    25441 cggcgtagcg ttatcggtaa tgcccagcgc caccggtacc cacagcacca acaggaaacg
    25501 cagcgtgaca cccgctcccc agaataaact ggtgcccacc agcgaaaaac gcgtttcacc
    25561 attgcaccat agcgaggtgc aggcattcag gaaactgcgg gtcatgttga tgagattcca
    25621 ggactgcccc ggacgcgccg ccagtttggg aatgtagata ttggcaacga ccgcaccacc
    25681 gtaggccagt gcgcatgcgg ccagagcgac caggacatgc cagtcagcca gcacaccacc
    25741 ggctacggaa ccgagtaaaa tcgccgctat ggcagaagct tccattaaac cgttagcttt
    25801 tactaactta ctacccgtgg ttaattcgcc gagaataccg tatttcgccg gtgaataggc
    25861 tgcagcacca acacccacca gcgtatagcc gagaaacgga ttgataccaa agcagatact
    25921 ggctgcgccc agcagcttca ggccgttggc aaacatcatc acccggcctt tggcgaagct
    25981 atccgccacc tgcccgacaa acggcgccaa aagaatgtaa gcacctacaa acaccatttg
    26041 caggatgggc tggctccact ccggatagaa ctgcgctttc agtaacgcca gagtggcaaa
    26101 cagtagcgcg ttatcgccaa acgcagagag aaactgcgcc acgataaccg ctttcatccc
    26161 cttcgaccac aacgaagtgt tagtgtgcac tgactcactc atcgtgttgt tccgcttcgt
    26221 ctacccagct tttcaacgtg acaaagtcag gtttgccgct gccaagtaat ggcatctgtt
    26281 tcagatagcg aatatcgcgc ggtacagcaa gctccggcac gccgtgctcg cgggcatact
    26341 gttgcaactt atcgcgcgtc agttcgttat ctgtggtgaa aagcaccagt gcctcgcctt
    26401 tgctggcatc gctcttaatc gcagtggcat ggactttatc tggcgaaaca ccaagtgcca
    26461 gttgttccac catttccagc gacaccattt cgcctgcaat tttggcaaag cgttttgcgc
    26521 ggccctgaat ctgcacaaag ccctgctcgt caaaacgcac aatatcgcca gtgtcatacc
    26581 agccgcgctc catttcgccg cgaacattct cggcggtggg cacttccagt acacctggct
    26641 tctccacccg cagatagccg ttcattatgt tcggcccttt cagttgcagg cgtccgccct
    26701 cttcgatacc agggaccgac aacaggcgtg catccattcc tggtagaata cgccctaccg
    26761 taccgggttt cgccgccatc ggtacgttga tagaaacgac aggcgcgcat tcggtcacac
    26821 cgtagccttc aaggatgcgc aggccaaatt tatcctgcca aagctgtttg gtactttctt
    26881 gtagtttttc tgcgcctgcc accacatagc gtagacgata gaagtcatac gggttggcga
    26941 aacgcgcgta gtgaccgagg aaagtcgagg taccgaacaa cacggtgcaa ctgcggtcat
    27001 acaccaagtc cggcacaatg cggtaatgca gcgggcttgg ataaaggaac acttctgcac
    27061 ctgtaagcag tggcgtaaac aggcctaccg tcagcccaaa ggagtgaaac agcggtaatg
    27121 ccgacataaa gcgatcgttg gtggtgaagt cggcaatcgt tttaatctgc tcgacgttcg
    27181 ccagaatgct tttatggcta tggacgacgc cttttggatg gccttcagaa ccggaggtaa
    27241 agaggatcag cgcctcttct tccggctgct gttttacctg cgccaggcgc ggcatcagca
    27301 aatgagcgaa gatccatact ttgtcggcag tggtgacatc tgcttttaaa tcttccagat
    27361 agacccagcg cacctgagta agttgctccg gcagatgcca gagtttgcct ttatcgagaa
    27421 actggcggga agtgaagatg gttttgattt cagccgccgt aatagcactg gtcagccctt
    27481 ttaccccggc agtgtagttc atcattgcgg gaatgcggcg acgcgcgatg gccccaaaaa
    27541 tcactgccgc actgatgcct gcattgggca gcattaagcc gatgcgttcg ccttcaacac
    27601 tgtatttttc aaggatgcgt ccaacaaaca gcgttttcgt aagcaatttg cgataggagt
    27661 ctggggtaaa gttgacgtct tcgacacatt tcttcccggc tccgaagcgg tacattgcac
    27721 tcagtaaaga ttcgtacaga gtttcacgcg ggcgcaccgc catacgcgct tccatcatta
    27781 tttgatgcag catttcgcca gcgattttgc gacggtcacg ggcacgcggc gcatccggca
    27841 tcgccacctg cgttggaggc aaaatatgca gagtaatttg cgggaacaag cgacgtttaa
    27901 ccagaccttt caggcggctg aagtgcgtaa gttccgcccc ttcaatacgc acaggaataa
    27961 ccgttgcacc agacttcgcc gcgacaaaac ccgcgccatc gtagattttc atcagcgagc
    28021 ctgtagtggt gatgcgtcct tcagggaaaa tcaccactgg tcggccctgt tccaccagac
    28081 gtaccagatg tttaatagcc ataggttgcg tcgggtcgag aggaacaaag tcgataaatg
    28141 atttcagcca acgcatatac cactgttggc ttattgaggt gtaaacggca aacactggac
    28201 gcacaggtaa aaacagtcca agcaaaatgc catcaataaa agagacgtga ttaggcgtaa
    28261 ttagtacgcg ctcgcccttc agtgcctggg tgtcacccgt aacgcgaacg cgatacaaaa
    28321 cacggcacaa atttcgaaaa aagctaaaaa gcattccaac tccctttgct ctgattcagt
    28381 aaaagcgaat ggagggagat tacacgagat aaagaacgcg agcgacagta aattaggtgc
    28441 gaaaaaaaac ctgcgcatcc gcgcaggttg gtgcaagaga cagggtacga agagcgtacc
    28501 gaataatctc accaatcaat acctctggga tcttgattgt ggtctgcacg acgactcttc
    28561 gccagcgaga aaacgcaaag gaatgaaggg aaatgcaacg aggtgtgtaa attgtcggtt
    28621 actgttacag attgatgacc ggcaaaaaaa aacctgcgca tctgcgcagg ctggtgtaat
    28681 tcatgtgctc aacacgaagt tgacttcacc tatcaatacc tctgggatca ccactttagc
    28741 aacctgaagc caaacgccac cagcggtcaa tcgcaacagc gtttcgcaaa gtgtaaccaa
    28801 aggtttgcaa tctctttttt tgtcttactt atttgcgttt ggctcacatt cccacgatga
    28861 aaacacgcca ccccttgaac caacgggcgt tttccgtaac actgaaacaa tgtaagcgtt
    28921 tacccactaa ggtattttca tggcgaccat aaaggatgta gcccgactgg caggcgtttc
    28981 agtcgccacc gtttcccgcg tcattaataa ttcacccaaa gccagcgaag cttcccggct
    29041 ggctgtgcat agtgcaatgg agtctcttag ctatcacccg aacgccaacg cccgtgcgct
    29101 ggcgcagcag accactgaaa cggtcggtct gatcgttggt gatgtttccg atccgttttt
    29161 cggtgcaatg gtgaaagcgg tcgaacaggt ggcttatcac accggtaatt tcttattgat
    29221 tggcaacggt taccacaacg aacaaaaaga gcgtcaggct attgagcaac tgatccgcca
    29281 tcgctgtgct gcgttggtcg tccatgccaa aatgatccca gatgccgatt tagcctcatt
    29341 aatgaaacaa atgcccggta tggtgctgat caaccgtatc ctgcctggct ttgaaaaccg
    29401 ttgtattgct cttgacgatc gttacggtgc ctggctggca acgcgtcatt taattcagca
    29461 aggtcatacc cgcattggtt atctgtgctc taaccactct atttctgacg ccgaagatcg
    29521 tctgcaaggg tattacgatg cccttgctga aagtggtatt ccggccaatg atcggctggt
    29581 gacatttggc gaaccagacg aaagcggcgg cgaacaggca atgaccgagc ttttgggacg
    29641 aggaagaaat ttcactgcgg tagcctgtta taacgattca atggcggcgg gcgcgatggg
    29701 cgttctcaat gataatggta ttgatgtacc gggtgagatt tcgttaattg gctttgatga
    29761 tgtgctggtg tcacgctatg tgcgtccgcg cctgaccacc gtgcgttacc caatcgtaac
    29821 gatggcgacc caggctgccg aactggcttt ggcactggcg gataatcgcc ctctcccgga
    29881 aattactaat gtatttagtc cgacgctggt acgtcgtcat tcagtgtcaa ctccgtcgct
    29941 ggaggcaagt catcatgcaa ccagcgacta accgcagtta aagcaattcc agcgccagta
    30001 attcttcgat ggtctggcga cggcgaatca accgcgcctg accattatca aacagaactt
    30061 ctggtaacag cggacggcta ttgtagttgg atgacattga tgcgccatat gcccctgtat
    30121 catgcagtac cagataatca cctgccttca cttccggcaa ggcgcgggtt tcaacatttc
    30181 ccccttcctg ctgggtaaag acatcgcccg attcacataa cggcccggcg acgacggttt
    30241 ccaccgttgg cgcgtgttcc agagaacgac catcagctgc cagggcactg atatggtggt
    30301 aactaccgta cattgccggg cgcatcagat cgttgaaccc ggcatcaacc agcacaaagt
    30361 ggcggctacc catttgtttg acgctccgca cctgagtaat taatacgcca gactgcgcta
    30421 ccaggaagcg acccggttca atttccagtt tcacagggtg gcccaaatgg cgagcgattt
    30481 gctcacgcgc ggcattccac agaccataat aatgttcggt atcaaccgcc tcttcaccct
    30541 gttgataagg aatagaaagc ccaccgcccg cagaaatagc ctgtaaatcc tgaccgaatt
    30601 cgataacctg acgcaccata gcaccacaca cctgctccag atgggcataa tcaacgccag
    30661 aaccaatgtg catgtgaatg ccgactagct gcagatgatg acgttgtatc acgtccagtg
    30721 cggcgggcag atcggtgtac cagataccgt gcttgctgtt ttcgccaccg gtattggttt
    30781 tttggctatg tccgtgacca aaccccggat taacgcgcag ccatacccga tgccctggcg
    30841 aaacctggcc cagttggtcg agcgtatcaa cagaacccgc attcaccgga atttgcaatt
    30901 cactgacgcg ttcaagcgtc gcctgatcga taacatctgc cgtaaaaaca atatcatcgg
    30961 ggtgcgtttg cggattgtaa cccgccgcca acgcacgctc tatttcgcct aacgagacgg
    31021 aatccacttt cacgccctgc tcacgcatta agcgcaaaat atgaatattg gaacaggctt
    31081 tctgtgcaaa gcgcaccact tcaaactgtt tcagcgctgc aatctgccga cgaataattt
    31141 gcgcatcgta gacccacacc gggcagccaa attcagcggg caaacgcagc agattttcgg
    31201 cggtgagatc ggtatcggtg ctgaacagtg aatgtggcat aacaaactcc agatgagtgc
    31261 ttttttatga ttacgccaca tcataaaaag aataaaaaat atcgatttat gtcgagtcta
    31321 tgcaaaaatg atatggatta ccggattgcg agagagcgct aatggccgcc gttaacttac
    31381 gtcatattga aatttttcat gcggtaatga ccgccggaag cctgactgag gcagcacacc
    31441 tgctacacac ctcacagcca accgtcagcc gcgaactggc gcgctttgag aaggtgatcg
    31501 ggctgaaatt gtttgagcgc gtacgtggac gattacatcc taccgtgcaa ggactgcgtc
    31561 tgtttgaaga agtgcaacga tcctggtacg gactggatcg cattgtcagc gccgcagaaa
    31621 gtctgcgcga gtttcgccag ggagaactgt ctattgcctg cctgccggtc ttttcgcaat
    31681 cttttttacc gcagctcctg caaccctttc tggcacgtta tcccgatgtc agcttaaata
    31741 tcgtgcccca ggaatcaccg ctacttgaag agtggctctc ggcccagcgt catgatttag
    31801 gactcactga gacactccat acgcctacgg gaacagaacg taccgaatta ctctctttag
    31861 atgaagtgtg tgtgttacct ccgggtcatc cgctggcggt aaaaaaggta ttaacgccgg
    31921 atgattttca gggtgagaat tacatcagcc tttcccgtac tgacagctat cgccagttgc
    31981 tggatcagct atttactgaa catcaggtta aacgacgcat gatcgtagaa acccacagcg
    32041 ccgcgtcagt ctgcgcaatg gtacgggcgg gggtaggtat ttcggtggtt aacccgctca
    32101 ccgcactaga ttatgcggca agcggtttag tggtgcggcg gttcagtatt gcggttccgt
    32161 tcaccgtcag cctgatccgc cccctgcacc gcccgtcatc agcgctggtt caggcgttta
    32221 gtgggcattt acaagcgggg ttaccgaaac tggtcacttc tcttgacgct attttgtcgt
    32281 cagctacgac agcataaaag cgacagcatc ctcggcatgg atcgccgcgg tatcaaacac
    32341 aggcaaaaca ctgcgctctt ctggcaccag taaaccaatt tctgtgcagc caaaaatgac
    32401 gccctgtgcg ccctgttctg caaggcgagc aatcacttgc gcataataag cgcgtgacgc
    32461 ttcggtaaat tgccccagac acagttcttc aaaaataatc tgattaattt tcgcccgttc
    32521 atccgcttca ggaataagac aattgatgga aaattgttcc gtcagccgcc cgcgataaaa
    32581 atcctgttcc atggtgtaac gcgtacccag cagcgccaca cgagtcattc cggccccggt
    32641 aattgcacgt ccggtggcat ccgcaatgtg taagaaaggc agagtgcaac gtgactcaat
    32701 ggcatccgcc actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg
    32761 ctgtaagcca agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg
    32821 ctggcactct tctatttcat gaaaatcgac gctatgtagc agcatttgcg cagagtgaag
    32881 cccaccaagc cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc
    32941 ccagctcatt cctcccagca aaccaattgt tttcattcct ctctcctgat gtgtgttacc
    33001 gcaacaaggc tgaataagga acgagacaaa cgcctcaacg gccaggtgcc caatctctat
    33061 taacgaaaaa agggccggat gtacagcaca tccggcccgt gaaatcagac gccgatattt
    33121 ctcaacttct cgcctgccat cagtttgcgt tcgatatgtt ccagcgtgac atttttggtt
    33181 tccggaatga gccagaaagt aatacccaca aacgcaatgt tcagcgcagt gtagagccag
    33241 aacgtaccgg cagcgccaat gctatcaagc agtgtcagga aggccgcgcc gataatcata
    33301 ttcgacaccc agtttgtcgt cgtcgaacag gtaataccga aatcgcggca tttcagcggc
    33361 tgaatttcag agcacaggat ccacaccact ggcgcggcgc tcatcgcata accggcaata
    33421 cacatcatcg tcatgccaac agagagccag gacaagccac tggaagccgt accgttatca
    33481 aactgcatca ggcaatagcc cagcaccaga gtgcctaacg ccatcacgct gaaaccaatt
    33541 ttcagagccg gtttacgacc tgctttatct accgtaaaga ccgcaatgaa ggtcgcgaac
    33601 ataaaggtca gtcccacgac cagagtcgca atcatctgtt gttctgtggt cgtaaagccc
    33661 gccattttga agatacgcgg cgcgtagtac atgatgatgt tcataccggt aaactgctgc
    33721 atcgcctgca acaacatacc gagaaacaca gcacgacgga cgttacggtt gatcttaaac
    33781 agtgcccaac cgccctgttt taacttcagg ctttcacgaa tttcgttgag ttcttctcgc
    33841 gctttttccg acgtatcgcg cagcatacgc aacacttctt ccgcctcaat atgacgcccc
    33901 ttttccgcca gccagcgcgg gctatttggc aggaagacca ccagaataat cagcagaact
    33961 gctggtaaag caagaacccc caacattgcg cgccagttac cgctataact gaacgctgta
    34021 tcggataaaa acgccagcac gatgccgagt gtgaccatca actggtacat actgatcatc
    34081 ttaccgcgaa cgttttcact tgccatttca gaaagataca gaggagcggt gtaagacgcg
    34141 atcccgacag caatgcccag caccacacga gcggcgatta acatctctac gctggtcgca
    34201 aaagcggacc ctatagaacc gagtacaaac aggatggccc ccgccatcag gctgtattta
    34261 cgccccaggc ggaacgacag ccaaccatta aacagcgcac caattgctgc gccgagcatc
    34321 atgctactaa ccacccattc ctgcaaacga ctggtcagca caaagtgatc ggtaatgaac
    34381 ggcaacgctc cggcgattac gccgatatca agaccaaata acaatcctgc gaccgcagca
    34441 gctaccgaaa caaacatatt catacgccgc gtatcacgca aagaacgtgg cgttaaagca
    34501 gattccgtat tgatagtaac cattttttcc tgccagcaga gagtaagaca ttgtgaaaaa
    34561 atacgtgaac aattcacaca ggtgtcaggt cggaaacagc ataaatatgg attaaattgc
    34621 tg
//
LOCUS       DAZPAB010000021        33211 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZPAB010000021 DAZPAB010000000
VERSION     DAZPAB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.021"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(22..1881)
                     /gene="gss"
                     /locus_tag="ACHUPG_000934"
     CDS             complement(22..1881)
                     /gene="gss"
                     /locus_tag="ACHUPG_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR0860645.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2086..2952
                     /gene="yghU"
                     /locus_tag="ACHUPG_000935"
     CDS             2086..2952
                     /gene="yghU"
                     /locus_tag="ACHUPG_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR0860646.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(2981..3062)
                     /locus_tag="ACHUPG_000936"
                     /pseudo
     CDS             complement(2981..3062)
                     /locus_tag="ACHUPG_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3075..3323)
                     /gene="hybG"
                     /locus_tag="ACHUPG_000937"
     CDS             complement(3075..3323)
                     /gene="hybG"
                     /locus_tag="ACHUPG_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR0860647.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3457..3798)
                     /gene="hypA"
                     /locus_tag="ACHUPG_000938"
     CDS             complement(3457..3798)
                     /gene="hypA"
                     /locus_tag="ACHUPG_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR0860648.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3791..4279)
                     /gene="hybE"
                     /locus_tag="ACHUPG_000939"
     CDS             complement(3791..4279)
                     /gene="hybE"
                     /locus_tag="ACHUPG_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR0860649.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(4272..4766)
                     /locus_tag="ACHUPG_000940"
     CDS             complement(4272..4766)
                     /locus_tag="ACHUPG_000940"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR0860650.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4766..6469)
                     /gene="hybC"
                     /locus_tag="ACHUPG_000941"
     CDS             complement(4766..6469)
                     /gene="hybC"
                     /locus_tag="ACHUPG_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR0860651.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6466..7644)
                     /gene="hybB"
                     /locus_tag="ACHUPG_000942"
     CDS             complement(6466..7644)
                     /gene="hybB"
                     /locus_tag="ACHUPG_000942"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR0860652.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7634..8620)
                     /gene="hybA"
                     /locus_tag="ACHUPG_000943"
     CDS             complement(7634..8620)
                     /gene="hybA"
                     /locus_tag="ACHUPG_000943"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR0860653.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8623..9741)
                     /gene="hybO"
                     /locus_tag="ACHUPG_000944"
     CDS             complement(8623..9741)
                     /gene="hybO"
                     /locus_tag="ACHUPG_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR0860654.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9930..10217)
                     /gene="yghW"
                     /locus_tag="ACHUPG_000945"
     CDS             complement(9930..10217)
                     /gene="yghW"
                     /locus_tag="ACHUPG_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR0860655.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10336..11223)
                     /gene="yghX"
                     /locus_tag="ACHUPG_000946"
     CDS             complement(10336..11223)
                     /gene="yghX"
                     /locus_tag="ACHUPG_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR0860656.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11380..12420
                     /gene="gpr"
                     /locus_tag="ACHUPG_000947"
     CDS             11380..12420
                     /gene="gpr"
                     /locus_tag="ACHUPG_000947"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR0860657.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12460..12954)
                     /locus_tag="ACHUPG_000948"
     CDS             complement(12460..12954)
                     /locus_tag="ACHUPG_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR0860658.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13145..14029
                     /gene="yghA"
                     /locus_tag="ACHUPG_000949"
     CDS             13145..14029
                     /gene="yghA"
                     /locus_tag="ACHUPG_000949"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR0860659.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14301..14726)
                     /gene="exbD"
                     /locus_tag="ACHUPG_000950"
     CDS             complement(14301..14726)
                     /gene="exbD"
                     /locus_tag="ACHUPG_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR0860660.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14733..15467)
                     /gene="exbB"
                     /locus_tag="ACHUPG_000951"
     CDS             complement(14733..15467)
                     /gene="exbB"
                     /locus_tag="ACHUPG_000951"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR0860661.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15719..16906
                     /gene="metC"
                     /locus_tag="ACHUPG_000952"
     CDS             15719..16906
                     /gene="metC"
                     /locus_tag="ACHUPG_000952"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR0860662.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            17046..17705
                     /gene="yghB"
                     /locus_tag="ACHUPG_000953"
     CDS             17046..17705
                     /gene="yghB"
                     /locus_tag="ACHUPG_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR0860663.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17745..18644)
                     /gene="yqhC"
                     /locus_tag="ACHUPG_000954"
     CDS             complement(17745..18644)
                     /gene="yqhC"
                     /locus_tag="ACHUPG_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR0860664.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18838..20001
                     /gene="yqhD"
                     /locus_tag="ACHUPG_000955"
     CDS             18838..20001
                     /gene="yqhD"
                     /locus_tag="ACHUPG_000955"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR0860665.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20106..20933
                     /gene="dkgA"
                     /locus_tag="ACHUPG_000956"
     CDS             20106..20933
                     /gene="dkgA"
                     /locus_tag="ACHUPG_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR0860666.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            21133..22059
                     /locus_tag="ACHUPG_000957"
     CDS             21133..22059
                     /locus_tag="ACHUPG_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR0860667.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            22110..22367
                     /gene="yqhH"
                     /locus_tag="ACHUPG_000958"
     CDS             22110..22367
                     /gene="yqhH"
                     /locus_tag="ACHUPG_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR0860668.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22410..24629)
                     /locus_tag="ACHUPG_000959"
     CDS             complement(22410..24629)
                     /locus_tag="ACHUPG_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR0860669.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24740..26152)
                     /gene="ftsP"
                     /locus_tag="ACHUPG_000960"
     CDS             complement(24740..26152)
                     /gene="ftsP"
                     /locus_tag="ACHUPG_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR0860670.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26227..26964)
                     /gene="plsC"
                     /locus_tag="ACHUPG_000961"
                     /gene_synonym="parF"
     CDS             complement(26227..26964)
                     /gene="plsC"
                     /locus_tag="ACHUPG_000961"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR0860671.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27198..29456)
                     /gene="parC"
                     /locus_tag="ACHUPG_000962"
     CDS             complement(27198..29456)
                     /gene="parC"
                     /locus_tag="ACHUPG_000962"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR0860672.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(30002..30484)
                     /locus_tag="ACHUPG_000963"
     CDS             complement(30002..30484)
                     /locus_tag="ACHUPG_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR0860673.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30537..30929)
                     /gene="ygiW"
                     /locus_tag="ACHUPG_000964"
     CDS             complement(30537..30929)
                     /gene="ygiW"
                     /locus_tag="ACHUPG_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR0860674.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            31081..31740
                     /gene="qseB"
                     /locus_tag="ACHUPG_000965"
     CDS             31081..31740
                     /gene="qseB"
                     /locus_tag="ACHUPG_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR0860675.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31737..33011
                     /gene="qseC"
                     /locus_tag="ACHUPG_000966"
     CDS             31737..33011
                     /gene="qseC"
                     /locus_tag="ACHUPG_000966"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR0860676.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(33057..>33211)
                     /locus_tag="ACHUPG_000967"
     CDS             complement(33057..>33211)
                     /locus_tag="ACHUPG_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2645 domain-containing protein"
                     /protein_id="HFR0860677.1"
                     /translation="AFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLFWG
                     RFIFCY"
BASE COUNT         8163 a         8887 c         8387 g         7774 t
ORIGIN      
        1 acggggttgc tacaacatgt attacttttt caccacaatt aacggttcaa tatcactctc
       61 ttttttgatg accagtgatt catcaccgcg caaacacgtc ccaccgtagt tgccgccaac
      121 ggtgaaggta catacctgaa tgtatttacc gtccactttc ggcaaacacc acagttgctg
      181 atagatgttt ttctgctcgg caaatttacc gctggttttg tccagcacct cttcatgatg
      241 gctgaccagg tcgatattgc taccacagcg accggcgatc ggtttcactg cgtaacctgt
      301 tttcaccaat tcatcattaa cagtgaaatc ggtatccagc aggtaacgat ggtgcgggaa
      361 cagcgaccag aggatcggca gaatcgcttt gttgccgggg atcaccgtcc acagcggctc
      421 aaagaccagc acttccgggc gcagcaatac gtcgataaga cgcacttcgt tttgcggatg
      481 accggtacgg attggcaccg cagcaaactc acggtcgcta acttcacgaa tctgatcaaa
      541 cgcggtttcc cacgcccagg ttttccacac gcagttaacc agtcgccctt ccccatcaat
      601 cagttgcccg gcagcatccc agcccagttc atccagcccg cgcaagatac gcgtttcaaa
      661 gcccgcctgg tgcagcgcct gctccataaa ctgcgcgtga tagttttcct cgatatcttt
      721 gtcctgcatg atatggacaa acggacgtgc acgactgtgt ttccaggcac cagccagttc
      781 gttaatcagc ccttccgccg gattgaagcc gttgccttta tagccctgct ccgcccaacg
      841 ttcgaggatc aagcccgctt cggtatgaca ggaggcggag tcggcgttgt attcgtaaac
      901 cttcaggcca cgctcatcca tgcagaaatc catacgacca gtgatcatat ggtgacggcg
      961 acgctgccag gagagacgca aacgtggcca gaggattttc ggaatgtcga acagcgccag
     1021 caggttgtca tctttcagca ccttgtcggt tgcgtgaaga tacatcagat gcagctcgtt
     1081 ggtggcttta attagctcct gctcggcact ctcggtaatg gtgtagtaat gatacggatc
     1141 ctgattgatc acctgaccgt tggcctgcac ataggcgttt tgtagcggat ctttttcatc
     1201 cagccattta ccgtcaaact ggcctttgtt ttccagacgc gctccactga ttttcagcag
     1261 ctcgcctgca atttccggct gcggtaagct gtattcagta tcttccgtct ggatcatcca
     1321 gcccagaatg gtggtgtcat caaaagtgtc tttaagggta tagcagccgt tttcgaccac
     1381 catctccagc tcgcgcgtcc actgttgccc ttgcggcaac ggggaatgaa tcacgttctg
     1441 ttccgcaata cggactttgt tgccatgcaa ttgggtaatg atggcgacat ggccagtgtc
     1501 tttaaattca ccgcctttat cccagataag aagcgcaccc gcgaccggcg cacgcggcga
     1561 gccgttagga aatgcctgca atggcaggat gttgtcatta accacttctc gcaggaagcg
     1621 cagcgagaaa atctcccacg ccatacccac gtcagtaaag accacaccgt aattcagaaa
     1681 gagaaaacgg cgagcaaatt caacgcattg ccacttgtgg cccatatatt cgtcgtcgat
     1741 atagctacgg aatacggcgt catcttcgta ttcctgcgga tcgagagaac tgtaatctga
     1801 agagtagatt gctaccccac ctggggcgta gcccaataat gtcccgaacg gggcatcctg
     1861 gctggtcgtt cctttgctca tcactttacc tttaacaata caacctaagc agttggcgta
     1921 attgttgtgc tctgattacc tgctcagcga cattaaccgg acagaggtca gactaatcat
     1981 acacctcatc gcagcggctg gcgcaaaggg ttaaaaaatt cacattctgt acagcaagtg
     2041 acctgctaca ctgcttcaac actaccactc agaaggcaac tcactatgac agacaatact
     2101 tatcagcccg cgaaagtctg gacgtgggat aaatccgctg gcggcgcgtt cgccaatatc
     2161 aatcgcccgt tttctggtcc gacgcatgaa aaaacgctgc ccgttggcaa acatccattg
     2221 caactttatt cgctgggaac gccgaacggt cagaaagtaa cgattatgct tgaggagcta
     2281 ctggcgctgg gcgttactgg tgcagagtac gacgcctggc tgattcgtat tggcgatggc
     2341 gatcaattct ccagcggctt tgtcgaagtg aacccaaact cgaagatccc ggcgctgcgc
     2401 gatcatacgc ataatccgcc gatccgcgtg tttgaatctg gttcgatcct gctttatctg
     2461 gcggagaaat ttggctactt cctgccgcag gatctggcaa aacggactga aacgttgaac
     2521 tggctgttct ggttacaggg tgccgcgccg ttcctcggcg gtggttttgg tcacttttac
     2581 cattacgcgc cggtgaaaat tgagtacgcc atcaaccgct ttaccatgga agccaagcgc
     2641 ctgctcgacg tgctggataa gcaactggcg cagcataagt ttgttgcggg cgatgagtac
     2701 accattgcgg atatggcgat ttggccgtgg tttggcaacg tggtgctcgg cggcgtgtat
     2761 gatgccgcag agtttctgga tgcgggaagt tataaacatg tgcaacgctg ggcgaaagaa
     2821 gtgggcgaac gtccggcagt gaaacgtggg cgtattgtta accgcaccaa cgggccgctg
     2881 aacgagcagt tgcatgagcg ccatgacgcc agtgattttg agacaaatac agaagataag
     2941 cgtcaggggt aagggttggt gttcgtcgca gcaagccatc caggccggat aaggcgttca
     3001 cgccgcatcc ggcaatcgtg cacaatgcct gatgcgacgc tgccgcgtct tatcaggcct
     3061 acaaaagcta atcatcacgc gctggtaatg tcgtaatcca tttggcgcag tgcgtctaat
     3121 gtggctttgg cttcatcttc gtcgatgatg ctcatggcaa atccgacgtg taccagcacc
     3181 cactggccca gcagatcggc agggttacct tcgcaaatca gggcgatatt cacgtcgcgc
     3241 ttgataccac atacttcaac ctgcgcaagc tggtgaatat cttcaccgac agccagcacc
     3301 tggcctggaa cgccaataca cattgttaac tccggttatt tcgattgccg gacaaacgcg
     3361 aaaaagaacc tttagcgggt gttccctctc accctaaccc tctccccgga ggggcgaggg
     3421 gaccgtccgt gcgcttttta ggcgttgtcc tcagtcttat tcaacttcaa tacttttgac
     3481 gatcagcgaa tcgccggtat cgacacgcaa ccgctcgccg tgacagagcg gacactgcgc
     3541 atcgtgctga tgaatctcca ccacctggct gcaatcccag caccaggcct gggcgggttt
     3601 atagacgata tgtaaatcgc acccttgcgc caccgttccg tggcagacaa tttcaaaact
     3661 aaaacggacg gcgctctcct caacgcagga gagcgcgcca atttccagcc acacggcggt
     3721 gacgcgctta acatcgtgct gctccgcctg ccgttggata atttcaacgg cgctctggca
     3781 aagcgacaac tcatgcattt tcgcccctcc tgcggccaaa cagcagggcg cgacgccctg
     3841 cgtgtggtac atccggattg gtgactggca acgaaaggat cattcgtgcg cagtcatccg
     3901 tcaggcgttg gccttcttca atcgacatgc tgtgcgaaag cggcgacatc agcgaacagg
     3961 agaggtattg cgaaacaccg tccagttcgc caacggtaaa ggtcatggtg ccatacggca
     4021 gttgcagacc aattttttca ctcactttgc gcagcggcca gagttgatcc gggccgggga
     4081 agataactgc actcagcatc cacggggtga tcacacaccc cgtccactga ccttcgaaca
     4141 gcgtaaaatc agaaacatac accggcattg aaggatgcag aaaagagaga tcgtgcatcg
     4201 aacgccgggc aatttcttca aacgctgcct gtacttgcgc cttcggggag gtctggaaac
     4261 ctgctatctc ttcagtcatg aatcgcctcc cgtgggatgg cttccacgcc tgattcgcgc
     4321 agcgcagcca gaacctgctc aagcgcaggt tcaatcattg cttcaaccgt cggcgttaag
     4381 ccgatgtgtg gctccagcga ttccgggatc acgccgacca gggtcagctt tttcggaaac
     4441 tcaccggtga agcgcagggc cgacaatacg tcggccaggc caagctgatg cggagagatt
     4501 ttgttggtaa acaacgccgg aacttcttca tcccgcagga tcatcatcgt tcccggagtg
     4561 ttctttttcg acacaatggc atccgcaata atcagatgat cgcgatttgc catgtcgcca
     4621 agcagctcca ttcccgccgt gccgccatcg aggatctcaa cataatccgg cagaatgtat
     4681 cgttgctcta acgcttcgac aatccgcacg ccgatggctt catcggtcag caaaatattg
     4741 ccgaccccta agactaaaat acgcattaca gaaccttcac tgaaaccact tcgttgccgt
     4801 cggcatccac tacgtgtacc gcacaggcca tgcacgggtc gaaggagtga atggtacgca
     4861 ccacttccag cggtttattc ggatcggcaa ccggtgtacc caccagcgac tgctcgtaag
     4921 gaccgacgtc atcattgaag ttacgcggac cagagttcca ggttgatgga acaaccgcct
     4981 ggtagttgct gatgataccg tctttaataa ccatccagtg agagagcata ccgcgcggcg
     5041 cttcgaggaa gccaacacct ttgaactcac ccgttgccgg aatgttcggc ttcacaaagg
     5101 tggtgtgatc gcctttgccg atattggtga tcagtgcact gtattggttt tgcaggatat
     5161 cctgcaattc acagcagtga acggtacgac caataatacg gcccagcgtg gagtgcagct
     5221 gtgccacttc cagcgtgttg ccagtcagtt tctgataaat cgcaacgatt tcattcagtt
     5281 tgttctgggt agattcgcga cctgccgcca gtttcaccag catattagcc agcggaccca
     5341 cttctaccgt tttgccgtag aaagtcggtg atttcaccca ggaatatttc ccgtcgtcag
     5401 accaaccatc ataagccgga atggtggtac cttcccacgg cgcctgtggc gcttcgtctt
     5461 tataccagga gtgcttcgcg ctttcctgaa tgcctttgat caggtattca tcggaatgag
     5521 aagtgatcgg acgatacgag gacagatccg cattctcaat gtagcctccc gggaacagga
     5581 agctgccgtt tttgctgtcg gtcgggaatt ccggcacgct caggtagttc accgcacctt
     5641 taccgcgcgt cagccattcc gggtagaacg cggcgataac cgcggtatca accttataaa
     5701 cctgctcaac aaagtcgctc agtttgtcga tgaaagactt gatgtacatc aggcgctcaa
     5761 ggttcaacac gcccaacccg tcgaggttga ttgggttcgc gacaccaccc acagccaggt
     5821 tctgaatgtg cggcgtttta ccgcccagca gcgccacgac gcggttagcg tcacgctggc
     5881 actccagcgc ctgcaggtag tgcgctaccg caatcaggtt cacttccggc ggcagtttca
     5941 tcgccgggtg accccagtag ccattggcga aaatacccaa ctgaccgctg gcaaccagat
     6001 ctttgatctt gttctgaact ttagtgaact cttccggact gttcagatgc caggtcgaaa
     6061 cgcctttcag catttcggag gctttggttg ggtcagcttg cagtgcagaa gtgatgtcca
     6121 cccagtccag cgccgaaagc tgatagaaat gaacgatatg gtcatgcgtg gtgtgcgcag
     6181 ccagaatgat gttacggatg tattgcgcgt taaccggaac gtcgatattc agcgcacttt
     6241 ctgccgcacg aacggaagac agcgcgtgag tggtagtaca tacgccacag atacgttgca
     6301 caatcatcca tgcatcgcgc ggatcgcggt ttttcacgat ctcttccatg ccgcgccaca
     6361 tggtaccgga agcccatgct ttcgaaacga cgccattttc gatttcgcaa tcgatgcgta
     6421 aatgcccctc aatacgggtt accggatcaa tagtaattct ctggctcatg ctttgctcgc
     6481 ctcatgacga ttatgatcgt tttgttttaa aggaggaagt atcggcagta gacgaatgag
     6541 tacgatgtaa gcgcaaatct caatagccac aaaaccaata gaaatcaaca gttcttccca
     6601 ggtcgggaag taggcgtaac cgccgcccgg gttgaatgcc accaacgaat aggtcagacg
     6661 ccaggttgca caacctaaca gtgcgctcag tgctgacagg aacagcatac gggaatcatt
     6721 acgcagcttc gccacacgca gaacgaccag cgggaagagc atcagcagga cttcaatcca
     6781 gaacatcacg gagtagaagt caccggcaaa cgctaacgac agcttgtcgc gatagataag
     6841 ctcgccaaag cgcagcccga tgaaaatcgc cagcaacaca ctgatggtgt tggtcagctt
     6901 gacgaacagg ctcttttcat ccggaccgtt gccacgcaga cccgcctgca ccagtgaacc
     6961 ttcaaagatg acaatcgaga agcccatgat gaacgccgtc agcagcgaga acagcggcaa
     7021 catttcatag ctctgccaga gcggatgcac cttgtagccc gccgagatca tcagcgaccc
     7081 cattgaagac tggtgcatgg tcggcagcag cgcaccgagc gcgatgatga agaacatcac
     7141 cttgttcaga cgttgtagcg acaccttcca gcccagacgt tcaaacagtg ccggagcaaa
     7201 ctccagagcc atcacgccga tgtagatggt catacagacc gccgtctcga acagtaccga
     7261 gttcacgttg aagtgacccg gaatgtagaa gtacggcagg ttccagtagc gacccacgtc
     7321 gatagtgatc gacaagccac ccagtgagta accaaacaga ctcgccaaca gcgccggacg
     7381 caccagcgga tggtattgcc cacggttaaa gacgtatacc gcccacgcca gcgcccagcc
     7441 gccacaggca aagccggtgc caatcagcag gtcaaacgcg atccacacgc cccacgggaa
     7501 gccgccgttc aggtcagaga cagagcccag accgaacacc agacgcttca caatcaggag
     7561 catacagatg acgattaacg gtccaaaaat catgaccggt ttactgatga ttttgccgcc
     7621 cagcggttgt ggatcatgac tcatgatcgt ctcctccgtc gtgatggtcg tttttggtgt
     7681 tgcgacgaac cagcacggtt aagcccgcca gcacagccag tggtagcatc atgcctttat
     7741 acagggtgtg ttgaatattt tcggaacgcg caccggtaga aagatcgtcc agtttcggca
     7801 ggtcgagatt ttcataaggc acacccgtca gtaccagaac ctgagtaccg ccgccctctt
     7861 tctcgccgta cagatgcgga taatatttcg gcaccgtatg caggtaagtg tcgccagatt
     7921 tcagcgtctg acgcggatag tggtattcgc tgccaggctt cagcgccaga cgttttttcg
     7981 cctccgccat cagctcttca cgcgtaccga aaatcaccgc gcccgccggg cacacttcta
     8041 cgcagccagg cagaccgcct ttatcgagac gttccacacc tttctggttg cacagctcgc
     8101 acttatgcag cgcaccaaac gggttgttgt agtcgtactt cggcacgttg tacggacagg
     8161 cgaccatgca gtaacggcaa ccggtgcaca cgtctttgtc gtaatggaca atgccggttt
     8221 tcggatcttt tttcagtgca gagaccgggc acacagagac acagttcgga tcgacgcagt
     8281 gcatacactg tttcttaatg tacgcgtagc cgttctcctc ctggtctttg ttgacccctg
     8341 tgccgctggt ccacacctga atgatgttat tggtatacgg cgacagtttg tcgttgttcg
     8401 accaggtctg ttccccttgc gggttacgtt cagggaagtt gatatcctga cacttggtga
     8461 cgcaagcctg gcagcctacg cacaaggtcg agtcgtacaa catccccagc gatcccggaa
     8521 ttggcgggcg gttttcagca gccgcatgac tgacagacgg cagcgcgccc gtcagcaatg
     8581 ccccgcagga ggctgcttta ataaaattac gtctgttcac ggttattctc cccgtgagtc
     8641 agcgttatct ttcttttgct gacgacccag ttcacgcacc gccatcacgc tgacaccggc
     8701 aaccagccca accacaccgc cgagcaaacc aatagcgcct gcagagacgt tgccgccctc
     8761 tttagcgtta acatccggtt tctgtgaacg cggagtctga ttttcgacgt tggcaagctg
     8821 atggatgcct ttatggaagc cgataccttt ttcgttacag ccatagcacg ggtgaccaat
     8881 cgccaccggc cacacaccgc caacatcgca gaattgcagc gttgagcagt tgccgtaagt
     8941 ttctggccct ttacagccga ggtggtacaa gcaccagcct tcgcggtggc cttcatcacc
     9001 gaactctttg gcaaaacgac cagcatcgaa gtgcgggcga cgttcgcagt gttcgtgaat
     9061 cagacggcca taggcgaagg tcggacggtt tttgtcatcc agtttcggcg gtttgccgta
     9121 agtgatgatg tgcgcaacgg tcgcgaggaa gttgtgcggg ttcggcgggc agcccggaat
     9181 attgataacg gttttgcctg gcagaacttc ttgcaagctg actgcgccag ttgggttaac
     9241 tccagctgcg gcaacaccgc cccacgcgga gcaggaacca atagcgataa tggctgctga
     9301 gccttccgcc gctttgcgga tgtgatccac aatcggctca ccggcaacca tgcaataaat
     9361 accgttatct tttaatggga tggaaccatc caccactaac acatactgcc ctttgtactt
     9421 ctcgagagcg ttatgtttgt tctcttcgac ctgatgaccg aaggcggcgg aaagcacttc
     9481 gtgatattcc agagagatag tctccagcac gaggttttct accgttggat gcgttgcacg
     9541 aagcagagat tccgtacaac cggtgcactc ctgcgcgcca atccagataa ctggcggacg
     9601 ctgcgggtta gtaaccgatt cggccatctc tgcagcggct ttgctactta accccatggt
     9661 ggcggctaat gctgcacaaa gcttcatgaa atcacgacgg ttaatgccgt gagaatggat
     9721 gagggtgtta tctccagtca tttatagtta ttccgttgcg agaacctggc atatattttg
     9781 cctcaatcgc aaaatcaata atgcgatcga tgcgccattt accacacatt tattatggtt
     9841 atcgatatca tgatactgcg gcggggcgct aaaacgaagg gaagaagcat tagtgtaatt
     9901 aattaaagca agataatacg tatgtttgat caattttcga ggcgataacc ggccataaaa
     9961 aagcgcaacg tagaacagga attattctca cgaaagaaat ccattaccat ctctttatcc
    10021 agtccatagc ggcgcgtcag aatacccgct tcccaggcgc taagctggcg atctccggtc
    10081 ttttcgtaca tcctgtgtga gtagcctaat acaaaaccgc gtttataatc ggcacagaat
    10141 tttgtcacat taaccgcact gtcggcatgc gttgctttta atcccgccat taagccttta
    10201 ccaaaatggt tattcatcgt tcacctcatt cattatatat cgccatatat aacgatattt
    10261 taggcaaaga acgacttttg cgcacgctat tttttcatac ttgtttaaga cgtaatcccg
    10321 taagagataa acctatcagg agagatattt atcgaaccat ttcagtgtcc tttgccaggc
    10381 aagatcggcg gcagatttgt cataacgggg cgtggaatca ttatggaatc cgtgattaac
    10441 ccccggatag atataagcct cataaacctt attattgtct ttcaacgccg cctcgtaagg
    10501 aggccagccc tcgttgattc gggtgtccag ttccgcgaag tggagtagta aaggcgcttc
    10561 aatcttcgcc acatcggcag tgggtgcctg acgaccataa aacggcaccg cgcaggccag
    10621 ttccggatac gcgacagccg ccgcgttcga tacgccaccg ccatagcaaa atccggtaat
    10681 acccacttta cctgtcgctt gcggatagcg ttgcataaac tcaattgcgg caaagaaatc
    10741 attcatcagt ttggttggat caacctgctg ttgcagctca cgacctttat catcatttcc
    10801 cggataacct ccaacggaac ttaatccgtc aggtgccagg gcgatatacc ccgccttcgc
    10861 cactcgccgt gccacatctt cgatatacgg attcagtcca cgattctcat gcaccacccc
    10921 cacggctggc gttttgccgc tcatttttgc gggtttcacc agataacccc gtacctcgcc
    10981 gtgaccattt ggcgaaggat acgtgatgta ctcagcaaca atctccgggt cggtaaactc
    11041 tacctgagtc gccagcgcat aatttggctt gagcaaatca aacaacgcta atgccgtcat
    11101 cccgcccacc gcatacttcg ccgcaaggtt gaggaactca cgtttcgaaa ttttcccgtg
    11161 aacgtaatag tcgtagtaat ccaacaactc ttgtgggaaa tctttggcgg ttaaacgcgg
    11221 catcgttgca ttcctcaact ggtgtttttt taagcaaagc ataagcgcgt gtttttgccc
    11281 agtttttcgt cactctgtga gccagactac gggatacgcg ctggcgaatc gctaaactag
    11341 aaacattgtt tcgaaattga acggtggaaa ggagaggtca tggtctggtt agcgaatccc
    11401 gaacgttacg ggcagatgca gtaccgctat tgcggaaaaa gtggtttacg cctgcccgca
    11461 ttatcgctcg gtttatggca caatttcggt cacgttaacg cgctggaatc acagcgtgca
    11521 atcctgcgta aagcgtttga tttaggcatt acgcactttg atttagccaa caattacggg
    11581 ccgcctccag gaagcgcaga agagaacttt ggtcgcctgc tgcgcgagga ttttgctgct
    11641 tatcgcgatg aactgattat ctctaccaag gctggctacg atatgtggcc cggcccgtac
    11701 ggctctggcg gttcacgtaa atacctgctc gccagcctcg accaaagcct gaagcgtatg
    11761 gggcttgagt atgtcgatat cttttactct catcgcgtcg atgaaaatac gccgatggaa
    11821 gaaaccgcct ctgcgctggc tcatgcggta caaagcggta aggcgctgta tgtcgggatc
    11881 tcctcttact cgccagagcg gacgcaaaaa atggtcgagt tgctgcacga gtggaaaatt
    11941 ccgcttttaa ttcatcaacc ttcgtacaat ttactgaacc gctgggtgga taaaagcggc
    12001 ctgctggata ccctgcaaaa taacggcgtg ggctgtattg cctttactcc tctggctcag
    12061 ggattgctga ccggaaaata tctcaacggc attccgcaag attcacggat gcatcgtgaa
    12121 gggaataaag ttcgtggtct gacgccgaaa atgcttaccg aagccaacct caacagcctg
    12181 cgcttattga atgaaatggc acagcagcgt ggacaatcaa tggcgcaaat ggcgttaagc
    12241 tggttgctga aagatgatcg cgtgacgtcg gtattgattg gtgccagccg cgcggagcaa
    12301 ctagaggaga acgtgcaggc gctgaataat ctgacattta gcaccgagga gctggcgcag
    12361 attgatcagc atatcgccga tggcgagctg aatctgtggc aggcgtcgtc cgataaatga
    12421 cctgttaata acgggcgcgc gaaccgcgcc cgaatcagat cagtgattat gacgagtcag
    12481 tcggtcaaga tagcccatca caaatgcaga aagcacaaac gtcagatgga taatgacgta
    12541 ccacatcagt ttgttatccg ggacattttt cgcatccata aagacgcgca gtaagtgaat
    12601 ggaagaaatt gccacaatcg acgctgctac tttgtttttc agcgacgttg cgtccatttt
    12661 ccccagccac ttcagcttct ctttgttctc ggagatatcc agctgtgaga cgaaattctc
    12721 ataaccggaa aacatcacca tcaccagcaa accgccaacc agcgtcatat ccaccagcga
    12781 cagcaacacg aggatcaaat ctgattccgc catcgagaag atattcggca gtacgtgaat
    12841 aatctcctgg aagaacttca gcgccagggc aactaacgcc agcgaaaggc caaagtacac
    12901 gggggcaagc agccagcgag aagcatacat tgcattttca agaaaacgtt ccatacattc
    12961 ctgtcgtgta gttaaaacgg gcaacagtat atcgcaattg agtaaacagc acgcaacagc
    13021 aaatcaacat atacgttaaa cctttgtcgc ataattctga ggtttaggga tcttcctggt
    13081 tcccttatca acgcatcgag agcgtctacc cttaaaggac gttgaacctg aagggagaaa
    13141 aacgatgtct catttaaaag acccgaccac gcagtattac actggtgaat atcccaaaca
    13201 gaaacaaccg acgccaggca tccaggcgaa gatgacaccg ataccggatt gcggcgagaa
    13261 aacctatgtt ggtagcggtc gcctgaaaga tcgtaaagca ctggtgacag ggggcgattc
    13321 cggaataggt cgcgctgccg ccatcgctta cgcgcgtgaa ggggctgacg tggcgatcag
    13381 ttatcttccc gtggaagaag aagacgctca ggatgtgaaa aagatcattg aagaatgcgg
    13441 acgcaaagcc gttctgctgc caggcgattt aagcgatgag aaattcgccc gttcgctggt
    13501 tcacgaagcg cacaaggcgt taggcgggct ggatattatg gcgctggtcg ccgggaaaca
    13561 ggttgccatt ccggatattg cagacctcac cagcgaacag tttcaaaaga cctttgccat
    13621 taacgttttc gcgctgttct ggctaaccca ggaagcgatc cccctgctac cgaaaggtgc
    13681 aagtgtcatc accacttcgt caatccaggc ataccagcca agtccgcatt tactggacta
    13741 tgcggctacg aaggcggcga ttctgaacta cagccgtggc ctggcaaaac aggtcgcgga
    13801 gaaaggtatt cgggtgaata ttgtcgcgcc aggccctatc tggacggcac tgcaaatttc
    13861 cggcggacaa acgcaggata agatcccgca gtttggtcag aaaacgccga tgaagcgtgc
    13921 ggggcaaccg gcggaactgg cccctgtata tgtttatctg gcaagtcagg agtcgagcta
    13981 cgtcaccgca gaagtgcacg gcgtgtgcgg cggcgagcat ttaggttaaa aaaatgcccg
    14041 gttgtgaaaa gcaaccgggc atcattgtga attcactctt acctgatgtg gcgaaaacgc
    14101 cttatccgcc tacaaacgca tgaagattca acatgttgca atagattttg taggcctgat
    14161 aagcgtagcg catcaggcat tgagcaccta atgccggatg cgtcgtgaac gccttatccg
    14221 gcctacaaac gcacgaagat tcaacatgtt gcaatagatt ttgtaggcct gatgagcgta
    14281 gcgcatcagg caattctact ttacttcgct ttggcggttt cttcgccgac cagacctatc
    14341 ttcaggtaac ccgcctgatg cagcgtatcc attaccttca tcaacgtctc gtaatcgacg
    14401 gttttgtccg ctcggaagaa gatggtggtg tctttcttgc cttcggttaa cgcattcaac
    14461 gccgtaatca ttgtttcatc ggtgaccgga tcgttaccga taaacatcga gttgtctgcc
    14521 ttcaccgaca gataaaccgg tttttccggg cgcggctgcg gcgtgctggt agaagcaggc
    14581 aagttcacct tcacatctac cgtcgctaac ggtgccgcca ccataaagat aatcagcaga
    14641 accaacatca cgtcgataaa cggcgtcacg ttgatatcat gcatttcgcc gttatcgtcg
    14701 aggttttcgt taagatgcat tgccatcgga cattatcctg cgcgtaattt ttgtgcgaca
    14761 cgaaccggat gcgcagcggc gctggcttcc agatctaggt cacggctttg cagcaacaat
    14821 acctgcgctg caacatcacc cagcatcgct ttaaagccgc caatctggcg tgcaaatacg
    14881 ttatagataa cgaccgcagg aatcgctgcc acgaggccga ttgccgttgc taacagagct
    14941 tctgcgatac ccggcgcaac gactgccagg ttagtggtct gcgtttgcgc gataccaata
    15001 aagctgttca tgatgcccca gaccgtacca aacagaccaa cgaacggaga aatcgcgccg
    15061 atggttgcca gatagccgtt accgcggccc atttgacgac ccactgcggc gacccgacgc
    15121 tccagacgga agctggtacg ttctttaata ccttcgttat cgtcgctgcc ttctgacagt
    15181 tccagctcat tctgcgcttc attgagcaaa tgcaggctta agcttttgct accaaaatca
    15241 gcggcgatat cgttggcctg gtttaaggaa cgtgcttcag ccagcagttg ctgctcgcgc
    15301 ttaaggcgac gcttctgatt gaagaactct acgctcttac tgaagaagat tgcccaggtg
    15361 actacagagg ccaaaataag cccaatcatc acgcacttaa cgacaatatc ggcgtactga
    15421 tacatacccc agacggaaag gtccgtctgc attaaattat tacccactgt gtatctccag
    15481 gacgcaagtc acaaaatctg cgcataataa tatcaaaacg acgtcgaatt gatagtcgtt
    15541 ctcattacta tttgcatact gccgtacctt tgctttcttt tccttgcgtt tacgcagtaa
    15601 aaaagtcacc agcacgccat ttgcgaaaat tttctgcttt atgccaattc ttcaggatgc
    15661 gcccgcgaat attcatgcta gtttagacat ccagacgtat aaaaacagga atcccgacat
    15721 ggcggacaaa aagcttgata ctcaactggt gaatgcagga cgcagcaaaa aatacactct
    15781 tggcgcggta aatagcgtga ttcagcgcgc ttcttcgctg gtatttgaga gtatggaagc
    15841 caaaaagcac gcgacgcgca atcgcgccaa tggcgagttg ttctatggac ggcgcggaac
    15901 gttaacccat ttttccttac aacaagcgat gtgtgaactg gaaggtggcg caggctgcgc
    15961 gctatttccc tgcggggcgg cagcggttgc taattcgatt cttgcttttg tcgaacaggg
    16021 cgatcatgtg ttgatgacca acaccgccta tgaaccgagt caggatttct gtagcaaaat
    16081 cctcagcaaa ctgggcgtaa cgacatcgtg gtttgatccg ctgattggtg ccgatatcgt
    16141 taagcatctg cagccaaaca ctaaaatcgt gtttctggaa tcgccgggtt ccatcactat
    16201 ggaagtccac gacgttccgg cgattgttgc cgccgtgcgc agtgtggtgc cggatgccat
    16261 cattatgatc gacaacacct gggcagccgg tgtgctgttt aaggcgctgg attttggcat
    16321 cgatgtttct attcaggccg ccaccaaata tctggttggg cattcagatg cgatgattgg
    16381 cactgccgtg tgcaatgccc gttgctggga gcagctacgg gaaaatgcct atctgatggg
    16441 ccagatggtc gatgccgata ccgcctatat aaccagccgt ggcctgcgca cattaggtgt
    16501 gcgtttgcgt caacatcatg aaagcagtct gaaagtggcc gaatggctgg cagaacatcc
    16561 gcaagttgcg cgagttaacc accctgccct gcctggcagt aaaggacacg aattctggaa
    16621 acgagacttt acaggcagca gcgggctatt ttcctttgtg cttaagaaaa aactcaatga
    16681 tgaagagctg gcgaactatc tggataactt cagtttattc agcatggcct actcgtgggg
    16741 cgggtatgaa tcgttgatcc tggcaaatca accagaacat atcgccgcca ttcgcccaca
    16801 aggcgagatc gattttagcg ggaccttgat tcgcctgcat attggtctgg aagatgtcga
    16861 cgatctgatt gccgatctgg acgccggttt tgcacggatt gtataacatt gccacttttg
    16921 gacaattttg cagacatttt attgtgaaaa gtcttaaatt gttgcgtccg ggatcaaggc
    16981 gtcccggacg attcaggagt acaataggca gataaaggct taaacgctgt tccacaggaa
    17041 agtccatggc tgttattcaa gatatcatcg ctgcgctctg gcaacacgac tttgccgcgc
    17101 tggcggatcc tcatattgtt agcgttgttt actttgtcat gtttgccacg ctgtttttag
    17161 aaaacggcct gctgcccgcc tcatttttgc caggcgacag cttgttgata ctggcaggcg
    17221 cattgattgc ccaaggggtt atggattttc tgcctacgat tgcgattctg accgccgcag
    17281 caagtctggg ctgctggcta agttatattc aggggagctg gttagggaat accaaaacgg
    17341 tgaaaggctg gctggcacag cttcctgcta aatatcacca gcgcgccacc tgcatgtttg
    17401 accgccacgg tctgctggcg ctgctggctg gacgttttct tgcatttgtc cgtacgctgc
    17461 tgccaaccat ggcgggaatt tccggtctgc caaaccgccg cttccagttt ttcaactggt
    17521 taagtggatt gctgtgggtc agcgtggtaa ccagttttgg ctatgcctta agtatgattc
    17581 cgttcgttaa acgccatgaa gatcaggtaa tgacgttcct gatgatcctg ccaattgcct
    17641 tgttaaccgc tggcttgtta ggcacgctgt ttgtggtgat taaaaaaaaa tactgtaacg
    17701 cctgacgatt ttccccgttc ccggctgctg taccgggaac gtatttaatt cccctgcatc
    17761 gcccgcattc ttgccgcatc ttcccccggc gtcacaccga agtaacgttt aaactcacgg
    17821 ctaaattgcg atgcgctttc atagccgacg cgcatcgctg ctgcgctggc cttcatgccg
    17881 tcatggatga tcatcatccg cgccttatgc agacggtaat tcttcaaata ctgcaacggc
    17941 gaggtgctgg tgacagactt aaaattatgg tggaacgccg atacgctcat gttggcttct
    18001 gccgccagtt gctcgacgct caggttttcg gtgtatttat tctcaattcg tttcagcacg
    18061 cggctaatca gactgaagtg agtctggcga ctgaccagcg ccagtaacgc gccgccgcaa
    18121 ggtccggtca gcacgtagta cagaatttcg cggatgatct gtttgccgag aatacgcgca
    18181 tccagtggtc gctccatcac gtcgagtaac cgctccgccg cgcataaaat ctcttctgat
    18241 aacgtggcgg agttaatccc gctggctgcc atcgacggct ggaaatgctc atcttcgcca
    18301 atgtccatca acagttcctg taactgcaaa atatcgacat tgagacgcaa ccctgccagc
    18361 ggcacctctg acgtggcata ggtttcgcac tcaaacggca acggcaccgt cagcagcagg
    18421 tattcattgg catcataacg aaacacgcgt tcattgatat aaccgatttt atgcccggaa
    18481 aagagaatta tgatgccagg ctcgtacatc accggtgtac gtgcgaaagg cgtctcgcca
    18541 tacaacaaac gcacatcggg caacagttct gacaaactat tttctttatt tttcagttta
    18601 ttaactttat ccgccagcaa gcgacaaatc tcttcacgtt tcatatcgcg taatttctta
    18661 ggaataatgc ggcaatttga ttgtgcgcaa ttttgtagca tttctccagc actctggaga
    18721 aataggcaag acattggcag aaatgagcat tgagagccag ggcgctggcg atcacaatga
    18781 aaaacatcag gcagatcgtt ctctgccctc atattggccc agcaaaggga gcaagtaatg
    18841 aacaacttta atctgcacac cccaacccgc attctgtttg gtaaaggcgc aatcgctggt
    18901 ttacgcgaac aaattcctca cgatgctcgc gtattgatta cctacggtgg cggcagcgtg
    18961 aaaaaaaccg gcgttctcga tcaagttctg gatgccctga aaggcatgga cgtgctggaa
    19021 tttggcggta ttgagccaaa cccggcttat gaaacgctga tgaacgccgt gaaactggtt
    19081 cgcgaacaga aagtgacttt cctgctggcg gttggcggcg gttctgtact ggacggcacc
    19141 aaatttatcg ccgcagcggc taactatccg gaaaatatcg atccgtggca cattctgcaa
    19201 acgggcggta aagagattaa aagcgccatc ccgatgggct gtgtgctgac gctgccagca
    19261 accggttcag aatccaacgc aggcgcggtg atctcccgta aaaccacagg cgacaagcag
    19321 gcgttccatt ctgcacatgt tcagccggta tttgccgtgc tcgatccggt ttatacctac
    19381 accctgccgc cgcgtcaggt ggctaacggc gtagtggacg cctttgtaca caccgtggaa
    19441 cagtatgtta ccaaaccggt tgatgccaaa attcaggacc gtttcgcaga aggcattttg
    19501 ctgacgctaa tcgaagatgg tccgaaagcc ctgaaagagc cagaaaacta cgatgtgcgc
    19561 gccaacgtca tgtgggcggc gactcaggcg ctgaacggtt tgattggcac tggcgtaccg
    19621 caggactggg caacgcatat gctgggccac gaactgactg cgatgcacgg tctggatcac
    19681 gcgcaaacac tggctatcgt cctgcctgca ctgtggaatg aaaaacgcga gaccaagcgc
    19741 gctaagctgc tgcaatatgc tgaacgcgtc tggaacatca ctgaaggttc cgacgatgag
    19801 cgtattgacg ccgcgattgc cgcaacccgc aatttctttg agcaattagg cgtgccgacc
    19861 cacctctccg actacggtct ggacggcagc tccatcccgg ctttgctgaa aaaactggaa
    19921 gagcacggca tgacccaact gggcgaaaat catgacatta cgttggatgt cagccgccgt
    19981 atatacgaag ccgcccgcta agctttttac gcctcaaact ttcgttttcg ggcatttcgt
    20041 ccagacttaa gttcacaaca cctcaccgga gcctgctccg gtgagttcat ataaaggagg
    20101 aacgtatggc taatccaacc gttattaagc tacaggatgg caatgtcatg ccccagctgg
    20161 gactgggcgt ctggcaagca agtaatgagg aagtaatcac cgccattcaa aaagcgttag
    20221 aagtgggtta tcgctcgatt gataccgccg cggcctacaa gaacgaagaa ggtgtcggca
    20281 aagccctgaa aaatgcctca gtcaacagag aagaactgtt catcaccact aagctgtgga
    20341 acgacgacca caagcgcccc cgcgaagccc tgctcgacag cctgaaaaaa ctccagcttg
    20401 attatatcga cctctactta atgcactggc ccgttcccgc tatcgaccat tatgtcgaag
    20461 catggaaagg catgatcgaa ttgcaaaaag agggattaat caaaagcatc ggcgtgtgca
    20521 acttccagat ccatcacctg caacgcctga ttgatgaaac tggcgtgacg cctgtgataa
    20581 accagatcga acttcatccg ctgatgcaac aacgccagct acacgcttgg aacgcgacac
    20641 acaaaatcca gaccgaatcc tggagcccat tagcgcaagg agggaaaggc gttttcgatc
    20701 agaaagtcat tcgcgatctg gcagataaat acggcaaaac cccggcgcag attgttatcc
    20761 gctggcatct ggatagcggc ctggtggtga tcccgaaatc ggtcacacct tcacgtattg
    20821 ccgaaaactt tgatgtctgg gatttccgtc tcgacaaaga cgaactcggc aaaattgcaa
    20881 aactcgatca gggcaagcgt ctcggtcccg atcctgacca gttcggcggc taacgtgcaa
    20941 attctcccgg tggcggtaat gttccgctac cggacttttc agaaatcatt tattcccctc
    21001 gcgtcccgcc cgttgttact cttccttgtt caggaatgcc aaatataagg acatcatcat
    21061 gcagagccgg aagctcttaa aagaacaact catctatatc cgggataaac gcaacggaga
    21121 ggtgaaaaac agatgaaaat aatacttctg tttttagcag ccctggcaag ttttaccgta
    21181 cacgcgcagc ctccttcaca gaccgtagaa caaacagtcc ggcagattta tcagaactat
    21241 aaatcagatg ccagtacgcc ttatttcggt gaaaccggag agcgggcgat aacttcagcg
    21301 cgtattcaac aggcgcttac cctgaacgac aatcttacac taccgggcaa tattggctgg
    21361 ctggattatg atccggtttg tgattgtcag gattttggcg atctggtgtt agaaagcgta
    21421 gcgataaccc aaactgacgt cgatcatgcc gatgccgttg tgcgctttcg tatctttaaa
    21481 gatgataaag aaaagaccac acagacactg aaaatggtgg cggaaaatgg tcgttgggtc
    21541 attgatgata ttgtcagcaa tcatggcagc gtcttacaag cagttaatag cgagaatgaa
    21601 aaaacgctgg ccgctttagc ttcgttgcag aaagaacagc cagaatcctt tgttgctgaa
    21661 ctctttgaac atattgctga ttatagctgg ccgtggacgt gggtggtttc cgactcttac
    21721 cgccaggcgg ttaatgcctt ctataaaacc accttcaaga cggccaataa tcccgatgaa
    21781 gatatgcaaa tagaacggca atttatttac gacaatccga tctgttttgg cgaagagtcg
    21841 ctattttcac gcgttgatga aattcgagtc ctggagaaaa ccgccgattc cgcccgcatt
    21901 catgttcgtt ttacgctgac caatggcaac aatgaagagc aagagctggt tttacagcgg
    21961 cgcgaaggta agtgggaaat cgctgatttt atccgcccga acagcggcag cctacttaag
    22021 cagattgagg caaaaaccgc cgccagatta aagcaatgag ctgaattaaa taacaattag
    22081 ccggaacaat aaataaaagg gaacactata tgaaaacgat tttcaccgtg ggagctgttg
    22141 ttctggcaac ctgcttgctc agtggctgcg tcaatgagca aaaggtcaat caactggcga
    22201 gcaatgtaca aacattaaat gccaaaatcg cccggcttga gcaggatatg aaagcactac
    22261 gcccacaaat ctatgctgcc aaatccgaag ctaacagagc caatacgcgt cttgatgcac
    22321 aggactattt tgattgcctg cgctgcttgc gtatgtacgc agaatgataa aaaaatcccc
    22381 ggcagcatgt cagttgccgg ggattttttt taacgtccaa ccgccgcttt agggcgtttc
    22441 ttcgcaccag cattcaccgg acgagattgc gtagacgacg ctttttttgc cgtagcaggc
    22501 gtctgacgct gagtcgccat cggcgtatgt ttagtcaacg ccggacgggt attgcggttc
    22561 tggcgacgag cttcacgcat ctcttcaatg gttggcgcag gcactaagca atcgcgacgg
    22621 ctgccaatca gatgcttttt gcccatcgct tccagcgcct ggcggattaa cggccagttt
    22681 gccggatcgt ggtaacgcaa caacgcttta tgcaaacgac gctgtttgtc gcccttcggt
    22741 acgaagacgc cttcgctctt ataaccaatc ttcgccagcg gttttttccc ggtgtaatac
    22801 atggtggttg agttcgccag cggcgatggg tagaagttct gtacctggtc gagacggaaa
    22861 cgatgctttt tcagccacag cgccaaattc accatatctt catcacgcgt accggggtgc
    22921 gcagagatga aatacgggat cagatactgc tctttacctg cctgtttcga gtaggtatcg
    22981 aacagctctt taaagcggtc atagctgccc atgcccggct tcatcatctt cgacaacggc
    23041 ccttcttcgg tatgttccgg agcaatcttc agataaccgc cgacgtgatg ggtcgccaac
    23101 tctttgatat agcgcggatc ttccacggcg atgtcataac gtacgcctga ggcgataagg
    23161 atttttttaa tgcctttcag atcacgcgca cggcgataga ggttgatcgt cggttcgtgg
    23221 ttagtgtcca tgtgcggaca aatatccgga taaacgcacg acaaacggcg acaagtttgt
    23281 tcagcgcgtg gcgatttgca gcgcaacata tacatgttgg cagttggccc accgagatcg
    23341 gaaatcacgc ccgtaaaacc tggaacggtg tcgcggatcg cttcgatctc attaatgatc
    23401 gaatcttccg aacggctctg aataatgcgc ccttcgtgct cggtaataga acagaaagaa
    23461 cagccgccaa agcagccgcg cataatgttg accgaaaaac ggatcatttc gtaagccgga
    23521 atacgggcat tgccataggc cggatgtggc acgcgcttgt acggcagcgc aaaaacgctg
    23581 tccatctctt cggtagaaag cgggatagca ggcgggttga tccacacata gcggtcaccg
    23641 tgtttttgca tcaatgcgcg ggcgcagcct gggttggttt cgtggtgcag aatgcgcgaa
    23701 gcatgggcgt acagcacttt atcgcccttc actttctcga aagaaggcag caatacgtag
    23761 gttttttccc acggtttctg gcgcggtggc tgcacgatta cagctttcgc ttcctgcttt
    23821 ttcggcgcaa caggtttgtt atccgcacac ggcaaatctt cgccatacgg atgcgggatt
    23881 gggtcgattt tccctagggt atcaagacgg gtggaatcca caccgctcca gccaggcagc
    23941 gcctctttta cgataattgc ggtattacgc acatcgcgga tttcactaat cggctcgccc
    24001 atcgccagac gatgcgctac ttccaccagc ggacgttcac cgttaccaaa catcagcatg
    24061 tcggctttcg aatctaccag aacggaacgg cgcacggtat cggaccagta atcataatgc
    24121 gcggtacggc gcaggctagc ctcaataccg ccgaggatca ccggcacatc tttccacgcc
    24181 tctttacaac gctgggtata aaccagcgtg gcgcgatccg gtcgcttacc cgcgacatta
    24241 tctggcgtgt aggcatcatc atgacgtaaa cggcgatcgg cggtatagcg gttgatcatc
    24301 gaatccatgt tgccagcagt aacaccaaaa aacagattcg gtttacccag acgcataaag
    24361 tcgtctttgc tgctccagtc cggctgggcg atgatcccga cgcgaaagcc ctgcgcttcc
    24421 agcatacgac cgcaaatcgc catcccgaag cttgggtgat cgacatacgc gtcgccagta
    24481 accaaaatga tgtcgcagct atcccagcca agttgatcca tctcttcacg agacatcggc
    24541 aaaaacggtg ccggtccaaa acaggcggcc cagtactgcg gccaggagaa caggtcgcga
    24601 tccggttgga tcagggagat agagctcatt ttgcttccag aaatgataaa aaaataatca
    24661 aaggccgggg attataagcc ggaacgaaag agaaatcgaa aggtattcca tactcgccct
    24721 cctcgggcga gtatgaagat tacggtaccg gattgaccaa cagttgccca atcgagccac
    24781 ggtccgccat ttccagcgtc tgactgttga agtagaacgg gaagtgcgcc caggaaggct
    24841 gaccgaaata aacaagcagc tccacttgtc cgtcaaccca aacggtatct ttccagcctc
    24901 tgtcttccgg gaacggcatc gcgccattca cgttacggat ctggaacatt acgccttcaa
    24961 tatggaacgc ttgcggctcg tccgcgcgta ccgtccagcg ttcccacgtt ccttgctgcg
    25021 cggtgacatc aatacggttg acgtcccaca gctgtccatt aatacccggg tcatcaccca
    25081 gactgatatc gcgactgcga attggcgaac cagccatgat ttcagttggc agcaagcgca
    25141 tcggaagact gtctgtgacc agcggcagaa ggccggttgg gcgcagcgtt agcaccaagg
    25201 tagaaaccag aatactggat ggctcaaaga agccgcgaat acgatcaaca atgctcgccg
    25261 cttcaccaca ggtgatcgac acttcatcgc cgttgctcat atccaccaga atctcgcggc
    25321 gctcgcccgg tgccagcgaa agttgcttca ccgatacagg agcaggcagg aatccctgat
    25381 cgccagaaat cacatgtaac gggcgaccat cgctcatctg tagttgatag cgacgagagt
    25441 tcgacgcgtt cagcagtcgc aagcgcaccc agccacgcga gacttcaacg tacgggcttt
    25501 gtacaccgtt aaccagcagc gtatcaccaa caaagccgcc gcttcccggt tcgttgtatt
    25561 ctggcgtacc aaagttatcc agccgtttat cctggataat gaccggaaaa tcatccacac
    25621 cataatggtt ggggataggc agcgacttgc tgacttcatc ttccaccagc cacattccgg
    25681 caaggccgtt atagacctgc tgagccgtgc ggttgggagt attggcgtga taccacagag
    25741 tagctgcgtt ctggcgaata ggcagtacgg gtgcccagtc agcgtttggc gacatcatcc
    25801 gtgccggacc gcccatcagc gggcctggta cctgtagccc ggccaccgtc attgagacat
    25861 tttctgtcag gcggttgctg taaataagct taacatcgtc gcccttccag acgcggatag
    25921 tcggccccag gtaacgacca ttgattcccc agaccgacgc gcgtgtccct ggcgtaaatg
    25981 accagtgcgc acgttgtaca gtcataaaca gcggttgccc acggcgagat tcaagtagcg
    26041 gcggaacggg tagcggttgc tgttgcccgg ctgcgctggc cttcagggga acagcgcctg
    26101 cacaaagtgc aatccccgat gcctgaatga actgacgccg actgagtgac atatttgctc
    26161 catgtaaaac tggctaaagt aataccgtgc aggaaagtgt tccccgcaaa aatacagatt
    26221 gccgacttaa acttttccgg cggcttcgcg ttctgcgact tctttatcga gctcggcgat
    26281 tttttgttcc attatcgaac gacaatgggc agccagctca cgaacctgat ctttgccata
    26341 ctgactgacg tcaattggcg gcagcatttc gacaatcacc agaccgttgt gcagtcgatt
    26401 aagattaatc ttattcgaag ttgtagagac gcacacggga ataatcggga cgcccgccgc
    26461 aattgccgcg tgaaatgctc cagtcttgaa cggtagcagg ccgcgaccac ggctgcgggt
    26521 tccttccggg aacatccaga tggaaatacg gcgttttttg aaatgattca cgacttccgc
    26581 aatggtgccg tgagctttag tgcgattgtt tctgtcgatc aataagttgc cggttaacca
    26641 gtacaactgc ccgaagaagg ggatccacag caagctcttt ttacctaccg tcaccgtcgg
    26701 cggttgcaca atgttcgatg ctgtcaccat gtcatagttg ttctggtggt tagcgatata
    26761 gatagcattg ccgtagcttt cagcatctgc aggtttgcgg cactcaactt tcaggccgaa
    26821 cagtggcgca agacggccaa acatatgccc aaaggtggcc acatgtttcg ggttacgcgg
    26881 gctgaaaagg cagtaaatgg agccgaatac acagactaag atgctgtaaa tcacggtaat
    26941 aataagacga aagatatata gcataacacc ctctgaaggt ctgagagact ggcattgtac
    27001 gtcacctgaa atcaggttag ggcattgtta aaagccgctc tctggaaaag cgtattgtta
    27061 atcgcaacgc atgaataaac aacggtttca agggaatttt tatcgtttcc gggggttgaa
    27121 tacgctgccg gatgacgact taacgtttca tccggcgttc cttgctagcg ggaggaaaca
    27181 gcgccctccc cggcatatta ctcttcgcta tcaccgctgc tggcacggcg aggagagtcg
    27241 atctcaacac gatcgatacg ctgcaaaccg cgcatcaacg taccgcggcg tccacgttcg
    27301 ccagtgactt tctgtagctc ttccggacgc agtttaattt tgcgtttccc aacatgaatg
    27361 gtcagcgtgc tttgcggcgg cagaacgtac agttgcgcca gaccatcctc gccacgcgcg
    27421 gcttctgccg atggaatgtt gataatcttg ttgcctttgc ccttcgacag ctgcggcaga
    27481 tcgcttaccg ggaacatcaa catacggcct gcctgagtga ttgccagcag catatcggaa
    27541 gcatcttcaa tcaccaccgg cggcataaca tgggcatttt ccggtaaggt gatcaaagcc
    27601 ttacccgcac ggttacgcgc taccagatcg ttaaaggtgc agacgaaacc gtaacccgca
    27661 tcggaagcca tcagcagttt ctgatcgtcg ctttccatca gcatatggtc aacggtcgcc
    27721 ccaggcggca acgttaattt gccggtgagt ggttcgccct gaccacgcgc cgacggcagc
    27781 gtaatcgggt cgatggcata gctacgaccg gtggaatcaa caaacactac cggttggtta
    27841 ctcttacctt tcaccgccgc tttgaagcta tcacccgctt tataattcag gcccggcgcg
    27901 tcgatatcat ggcctttagc gctgcgtacc cagcccatct gcgacagcac aatggtgaca
    27961 ggttcagacg gcagcatgtc gtgctcgctc atcgctttcg cttcttcgcg ttcctgcaac
    28021 ggcgaacgac gatcgtcacc gtaggcttgc gcgtctgcct gcagttcttt cttcagcagg
    28081 ttattcattt tacgctcgga agccaaaatg ccctgcaact ggtcgcgctc tttttccagc
    28141 tcactctgct caccgcgaat cttcatctct tccagtttgg caagatgacg cagtttcagt
    28201 tcgaggatcg cttccgcctg ggtttccgta aggccaaacc gcgacatcag cgccggtttc
    28261 ggttcatctt cgttacgaat gatctcaatc acttcgtcga tgttgagaaa cgccaccagc
    28321 aaaccttcca ggatatgcag gcgcttgagg actttctcca gacgatagtt cagtcggcgg
    28381 cgcacggtat cgcgacggaa caccagccat tcggagagga tttccagcag gtttttcacc
    28441 gccggacgac catccagacc gatcatattg aggttaatgc gatagctctt ttccagatcg
    28501 gtggtagcga agaggtggtt catcacctga tccatatcca cgcggttgga acgcggcaca
    28561 atcaccagac gggtcgggtt ctcgtggtca gattcatcgc gcagatcgtc aaccatcggc
    28621 agctttttgt tgcgcatttg cgcagcaatt tgctccagta cgcgcgcacc tgaaacctga
    28681 tgcggtaatg cgctgataac caccgcgcca tcttctttct tccacaccgc gcgcatacgc
    28741 actgaaccac gtccgttctc gtagatttta cgaatctcgg cgcgcgaagt gataatttcc
    28801 gcttcagtcg gataatccgg cccctgcacg atatccagca gctgatcgag cgtagttttc
    28861 ggctggtcga ttaatgcgat tgccgcctga gctacttcac gcaggttatg cggtggaata
    28921 tcggtcgcca tgccgacggc aataccggtg gtgccgttaa gcaaaatgtt tggcagacgg
    28981 gcaggtagca ttttcggctc ctgcaaagtg ccgtcgaagt ttggcaccca gtcagccgtc
    29041 ccctgcccca gctcgctcaa tagcagctcg gaatatttcg acaaccggga ttcggtgtaa
    29101 cgcattgccg cgaacgattt cggatcgtcc ggcgcgcccc agttcccctg accatcaacc
    29161 agcggataac ggtaagagaa cggctgcgcc atcaggacca tcgcttcata acaggcgcta
    29221 tcgccgtgcg gatggtattt acccagtacg tcaccgacgg tacgggccga ttttttaaat
    29281 ttggcgctgg cattcaggcc cagttcagac atcgcataca caatgcggcg ctgaacaggt
    29341 ttcagaccat caccaataaa cggcaacgca cggtccatga tcacgtacat ggagtagttt
    29401 aagtaggcgt tttccgtaaa ttcatgtagc gcaaggcgct ctgccatatc gctcattaat
    29461 tctgattcct caacttattc gccagccttc aaaggggcaa tattgccgga gatactacat
    29521 tatctgccgc gtcgagtcac aggagatcag cgtgaatagc gccgccggac gtgctccgac
    29581 ggcgataaag gcatcaatgt gccttgatat acaactcttt gctgtagact tgatcggcat
    29641 agagggcagc acaaggttgc gcccatgcca caccactcgg catgggggtc cgctccgggc
    29701 gattcgagaa gttgaggtta tgcgagtcca gggaagccta aagcatcaaa gtagtcgact
    29761 gtaagtatac ggttcagccc agtttagctg tgtctccacc agtgccccgc gcctcctgcc
    29821 ataagtgcgg cttgctcatg gatatccccg agacggctta cggcgcaccg gataataaat
    29881 ttcgcgtata catggtattc cttaccggag aaattataag cccaacaaca atacgcctgt
    29941 gacattgttc gctcaaactt atcgcgagtg atttggctca cactccggta aagattcccc
    30001 atcacgccaa cggcacataa acatccgttt ccagtcgctg ctctgtcacc ccttcggcaa
    30061 gattggtgta gtgaaacaga atcggtgctt tacgcatttt ctcgccgctt gcaggcagcc
    30121 agtggcgaat aatgccccat atcgtgtgtg aaatatcgtc cagctcgcca acgtggcggg
    30181 ccacggcata acgtccaccg gtaagttcac cattgctcac accataacga ttatcgggaa
    30241 tcggttcgct aacgctgccg cagatatcaa agcgaaacgc ttccggtgcg gtggtggcag
    30301 gggcgtccca ggcgacgcca aaagtctggc tttggttaac aggagaaagc cccgtctctt
    30361 tacgccacat gataaatttc gccacactgt agttgagcaa ctcagggcta cagcggtgcg
    30421 gcaacatcgc cacagggatt gatgggaaat cgataatgtt tacatccagt gtcaggtttg
    30481 tcattttgcc tccctgcgaa attattcccg ggaacgcgtc ccgggagcgg taacaattac
    30541 ggatttactt tgcggatctg tttgacgtca atttcaacag aattccagtc tttatcgact
    30601 tcaccctgaa tctcaaccgt atctttcggc gtcaccgtca caccgttcca gcgtttgtga
    30661 tcgatatcaa cattgatagt accgctggca tctttaaaca cgtagagatc gtcagagatg
    30721 cgttcaacga tattgccgcg cagggttacc caggtgtcgt cacgcaggga ttttgcgctt
    30781 tctacagtcg ttacgctgcc gttcggcccc tgaaatcctc cggcctgact ttgcgttacc
    30841 gatgggccag aaaaaccgcc ctgctctgct gccatcaccg gtgcactgca cagggccatt
    30901 actgcgatta ctgctgcgaa ttttttcatg tttattactc cctttaatgt ctgtttccga
    30961 gcatttaaca agatagtcct taacaacttc ttaagggaaa aaaataaaat ttaatgctgt
    31021 acagagcgcg ttacaacacg gtttactggc agcaaatacg gttatcgcag ggatgaaaaa
    31081 atgcgaattt tactgataga agatgacatg ctgattggtg acggcatcaa aactggcctt
    31141 agtaaaatgg gttttagtgt cgactggttt acacaaggtc gtcagggaaa agaggcgctt
    31201 tatagcgcac cttatgatgc ggtgatcctg gatttaacct taccaggcat ggatggtcgc
    31261 gatattttgc gcgaatggcg agagaaaggt cagcgtgagc cggtactgaa cctgaccgcg
    31321 cgtgatgcgt tagcggaacg tgtagaaggg ctgcgtctgg gagctgacga ttatctgtgt
    31381 aaaccttttg cgttgataga agtcgccgcc aggctggagg ctctgatgcg ccgaaccaac
    31441 ggccaggcca gcaacgagct gcgccacggt aacgtcatgc tcgaccccgg caaacgtatc
    31501 gccacgctgg ctggcgaacc cttaacactg aaaccaaaag aatttgccct gctggaatta
    31561 ctgatgcgta acgctggtcg ggtactgccg cgcaaactga ttgaagagaa actgtatacc
    31621 tgggacgaag aggtcaccag taatgccgtt gaagtgcatg tgcatcatct gcgacgcaaa
    31681 ctcggtagtg attttattcg taccgtgcat ggtattggct acacattagg tgagaaatga
    31741 aatttaccca acgtcttagt ctgcgcgtca ggctgacgct aatcttttta attctggcct
    31801 cggtgacctg gctgctttcc agctttgtcg cctggaaaca aacaacggat aacgtcgatg
    31861 aattgttcga cacccaactg atgctgtttg ccaaacggtt aagtacgctc gatctcaacg
    31921 aaatcaacgc ggcggatcgc atggcacaga cgccaaataa attaaaacac ggtcatgttg
    31981 atgacgatgc gctgaccttt gccatcttta cccacgacgg cagaatggtc cttaatgatg
    32041 gcgataacgg agaagatatt ccctatagct atcaacggga aggttttgct gacgggcaac
    32101 tggtcggtga agacgatcct tggcgttttg tctggatgac ctcacctgat ggcaaatatc
    32161 gcatcgttgt tggccaggaa tgggaatacc gtgaagacat ggcgctggcg attgttgccg
    32221 ggcaattgat cccgtggctg gtcgcactgc cgattatgtt aatcatcatg atggtactac
    32281 tgggtcgtga actcgcgccg ctgaacaaac tggcgctggc actacgtatg cgtgaccctg
    32341 actcggaaaa accactaaac gcgactggcg tacccagcga agtgcggcca ctggttgagt
    32401 cgctaaatca actgttcgcc cgcacacatg cgatgatggt tcgagaacga cgctttacct
    32461 ccgatgcagc tcacgaacta cgtagcccgt taacagcgct gaaagtgcaa accgaagttg
    32521 cgcagctctc tgacgatgat ccgcaggcgc ggaaaaaagc actgctccaa ttacattccg
    32581 ggatcgatcg cgctactcgt ctggttgatc aactgctcac gctatcgcgg ctggactcac
    32641 tggataacct tcaggacgtc gcggagatcc cgcttgaaga tctcctacaa tcgtcggtga
    32701 tggatattta ccacacggcg cagcaggcga aaattgacgt gcgactgaca ctcaatgccc
    32761 acggcatcaa acgcaccgga caaccgctat tgctaagttt gttggtgcga aatttgctgg
    32821 ataacggccc cggtgtgaca ccagaggcac tggcgcgaat tggcgaacgc ttctatcgcc
    32881 cacccggaca aaccgctacc ggcagtggac ttgggctatc gattgtccag cgaatcgcca
    32941 agctgcatga catgaatgtt gagtttggga atgcggaaca aggtggattt gaggcgaagg
    33001 taagctggta acttctctgt ctatgagagc cgttaaaacg actctcatag attttactaa
    33061 tagcaaaata taaaccgtcc ccaaaaaagc caccaaccac aaaccagaca aataacgagc
    33121 gagaatacat atagccaaaa ctcgaacatt tcccgcataa agagttttct taagataaga
    33181 ataataagtg gcgtaagaag aaaaaatgct g
//
LOCUS       DAZPAB010000022        32346 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZPAB010000022 DAZPAB010000000
VERSION     DAZPAB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.022"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..368)
                     /locus_tag="ACHUPG_000968"
     CDS             complement(<1..368)
                     /locus_tag="ACHUPG_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0860678.1"
                     /translation="MLRMFIPTSNGKISRHRYIFLFILINFIFAFLIIFFNDGEAGFL
                     VIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITMIAEDFACSGNGS
                     MIFLICYFSTFSMLMLAPTDS"
     gene            complement(463..1353)
                     /gene="rarD"
                     /locus_tag="ACHUPG_000969"
     CDS             complement(463..1353)
                     /gene="rarD"
                     /locus_tag="ACHUPG_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR0860679.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(1405..1872)
                     /gene="yigI"
                     /locus_tag="ACHUPG_000970"
     CDS             complement(1405..1872)
                     /gene="yigI"
                     /locus_tag="ACHUPG_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR0860680.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            2037..2906
                     /gene="pldA"
                     /locus_tag="ACHUPG_000971"
     CDS             2037..2906
                     /gene="pldA"
                     /locus_tag="ACHUPG_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR0860681.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3039..4868
                     /gene="recQ"
                     /locus_tag="ACHUPG_000972"
     CDS             3039..4868
                     /gene="recQ"
                     /locus_tag="ACHUPG_000972"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR0860682.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4932..5552
                     /gene="rhtC"
                     /locus_tag="ACHUPG_000973"
     CDS             4932..5552
                     /gene="rhtC"
                     /locus_tag="ACHUPG_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR0860683.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5614..6234)
                     /gene="rhtB"
                     /locus_tag="ACHUPG_000974"
     CDS             complement(5614..6234)
                     /gene="rhtB"
                     /locus_tag="ACHUPG_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR0860684.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6345..7367
                     /gene="pldB"
                     /locus_tag="ACHUPG_000975"
     CDS             6345..7367
                     /gene="pldB"
                     /locus_tag="ACHUPG_000975"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR0860685.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7375..8175
                     /gene="yigL"
                     /locus_tag="ACHUPG_000976"
     CDS             7375..8175
                     /gene="yigL"
                     /locus_tag="ACHUPG_000976"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR0860686.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8251..9150
                     /gene="bioP"
                     /locus_tag="ACHUPG_000977"
     CDS             8251..9150
                     /gene="bioP"
                     /locus_tag="ACHUPG_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR0860687.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(9038..9991)
                     /gene="metR"
                     /locus_tag="ACHUPG_000978"
     CDS             complement(9038..9991)
                     /gene="metR"
                     /locus_tag="ACHUPG_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR0860688.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10227..12488
                     /gene="metE"
                     /locus_tag="ACHUPG_000979"
     CDS             10227..12488
                     /gene="metE"
                     /locus_tag="ACHUPG_000979"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR0860689.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12527..13342)
                     /locus_tag="ACHUPG_000980"
     CDS             complement(12527..13342)
                     /locus_tag="ACHUPG_000980"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR0860690.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13604..14365
                     /gene="udp"
                     /locus_tag="ACHUPG_000981"
     CDS             13604..14365
                     /gene="udp"
                     /locus_tag="ACHUPG_000981"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR0860691.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            14506..15933
                     /gene="rmuC"
                     /locus_tag="ACHUPG_000982"
     CDS             14506..15933
                     /gene="rmuC"
                     /locus_tag="ACHUPG_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR0860692.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            16028..16783
                     /gene="ubiE"
                     /locus_tag="ACHUPG_000983"
     CDS             16028..16783
                     /gene="ubiE"
                     /locus_tag="ACHUPG_000983"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR0860693.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16797..17402
                     /gene="ubiJ"
                     /locus_tag="ACHUPG_000984"
     CDS             16797..17402
                     /gene="ubiJ"
                     /locus_tag="ACHUPG_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR0860694.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17399..19039
                     /gene="ubiB"
                     /locus_tag="ACHUPG_000985"
     CDS             17399..19039
                     /gene="ubiB"
                     /locus_tag="ACHUPG_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR0860695.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            19118..19387
                     /gene="tatA"
                     /locus_tag="ACHUPG_000986"
     CDS             19118..19387
                     /gene="tatA"
                     /locus_tag="ACHUPG_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR0860696.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19391..19906
                     /gene="tatB"
                     /locus_tag="ACHUPG_000987"
     CDS             19391..19906
                     /gene="tatB"
                     /locus_tag="ACHUPG_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR0860697.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19909..20685
                     /gene="tatC"
                     /locus_tag="ACHUPG_000988"
     CDS             19909..20685
                     /gene="tatC"
                     /locus_tag="ACHUPG_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR0860698.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20727..21509
                     /gene="tatD"
                     /locus_tag="ACHUPG_000989"
     CDS             20727..21509
                     /gene="tatD"
                     /locus_tag="ACHUPG_000989"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR0860699.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21506..21994)
                     /gene="rfaH"
                     /locus_tag="ACHUPG_000990"
     CDS             complement(21506..21994)
                     /gene="rfaH"
                     /locus_tag="ACHUPG_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR0860700.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            22161..23654
                     /gene="ubiD"
                     /locus_tag="ACHUPG_000991"
     CDS             22161..23654
                     /gene="ubiD"
                     /locus_tag="ACHUPG_000991"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR0860701.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23700..24401
                     /gene="fre"
                     /locus_tag="ACHUPG_000992"
     CDS             23700..24401
                     /gene="fre"
                     /locus_tag="ACHUPG_000992"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR0860702.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24593..25756)
                     /gene="fadA"
                     /locus_tag="ACHUPG_000993"
     CDS             complement(24593..25756)
                     /gene="fadA"
                     /locus_tag="ACHUPG_000993"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR0860703.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25766..27955)
                     /gene="fadB"
                     /locus_tag="ACHUPG_000994"
     CDS             complement(25766..27955)
                     /gene="fadB"
                     /locus_tag="ACHUPG_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR0860704.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            28145..29476
                     /gene="pepQ"
                     /locus_tag="ACHUPG_000995"
     CDS             28145..29476
                     /gene="pepQ"
                     /locus_tag="ACHUPG_000995"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR0860705.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29476..30090
                     /locus_tag="ACHUPG_000996"
     CDS             29476..30090
                     /locus_tag="ACHUPG_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR0860706.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            30129..31580
                     /gene="trkH"
                     /locus_tag="ACHUPG_000997"
     CDS             30129..31580
                     /gene="trkH"
                     /locus_tag="ACHUPG_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR0860707.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31592..32137
                     /gene="hemG"
                     /locus_tag="ACHUPG_000998"
     CDS             31592..32137
                     /gene="hemG"
                     /locus_tag="ACHUPG_000998"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR0860708.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7581 a         8193 c         8807 g         7765 t
ORIGIN      
        1 gaatcagtag gtgcaagcat tagcatactg aaagtggaaa aataacaaat caaaaaaatc
       61 atcgaaccat tgcctgaaca ggcaaaatct tcggctatca ttgtgatgat agagatgata
      121 tatactgcta atgtaccaaa aacataaatt tttatatata tgaaaccact atcacggagt
      181 cgttggcaat tcatgttgat gacgagataa tggagtgcga tggtagagac tataacaaga
      241 aaacctgctt ccccatcgtt aaaaaagata ataagaaagg caaaaatgaa atttattaaa
      301 ataaataaaa atatatatcg gtgtctggaa atcttaccgt tagatgttgg gataaacata
      361 cgtaacataa attttacatc cttgtgtgag tctcctgtca gcatggcaat atgcccactc
      421 tcatacaaga gtcggcatat tttttcagaa tatatttatt ttttatttgg acgttctacg
      481 ctgcgtataa atcgcgtcca tcacaaaaat tgccagcgcc acccaaataa aggtgaaagt
      541 caccatctta tcggcacccg gtttttcacc ataaaacgtc acagccagca ggaacatcag
      601 cgtcgggcca atgtactgaa aaaagcctaa cgttgagaga cgcaggcgcg tggcagcggc
      661 agtaaaacac aacagcggta cggtagtgac aataccggcg gcgatcagca gtaaattcag
      721 cgacatcggg ttttgcccca tatggctggt tgaactgtcg gcaatagcaa acaggtaaat
      781 cgccgccacg ggcagcagcc acatggtttc aattaacatg ccggtttgtg cttctaccgc
      841 aatcttcttg cgcaccaggc cgtagaaggc aaaactaaat gccagcccca gcgcgataat
      901 aggtagcgaa ccaaaagtcc acagctggac caacacgcca catatcgcca gaatcaccgc
      961 cagccattgc atccggcgga atcgctcgcc gaggaaaatc atccccagca caatgttcac
     1021 cagcgggtta ataaagtaac caaggctcgc ttccagcata tggtgattgt tcaccgccca
     1081 gataaacagt agccagttgc caccaatcag tacggcagag actgccagca taaaaatttt
     1141 ctgtggcgtc tgaatcagcg tttttaaata ggaccactgg cggcaaatgc tcatcagcac
     1201 caccataaag aaaaacgacc agatcacgcg atgcgtcagg atctcatcgg cgggcacgta
     1261 gtaaatcagc ttgaagtatg ctggcgcaat accccaaata aaataagcgg caagagcgag
     1321 taatacgccc tgccgcgttt gttttgcatc catcgggaat actcattttt aattggtaac
     1381 agcagtttac ctgcttttat gtcttcaacc taccatatag gtggcggtgg cactggcaat
     1441 ataaagctgt tcttcattgt gtaattcaac gcgggcgacg gcgactttat tgcctgcacg
     1501 caacaggcta ctggtagcag taaaacgctc gcccctgcct gggcgcagat aatcaacgcg
     1561 aagatcaatg gtccccatcc gcgatagccg ctggcgtagt tcatcttcac tgatggtttc
     1621 gtggcgggtt aaggtacttc ccacgcacac cagaccggcg gcgacatcca gcgccgacgc
     1681 aatgactccg ccgtgcaaaa tgctttgcgc ccagttaccc accatcatcg gctgattttt
     1741 aaaggccagc tgtgcgaact ctttttcgta acgctccagt tccatcccca atgcgcggtt
     1801 aaatggcatg tgataaacaa acatctcacc cactaatttc agggcttgtt cagcggtcag
     1861 tacggcagac atatcatcct tacacttcat tggttaatga aatgttgatt ttatgcttct
     1921 ttgttgttgg tttctacttt aggaagggat aactaacggc aatggagtta agtatgtaaa
     1981 atagcccgca gaaaaacatt caccttatca ataattcgtt acggagaaca cgaccgatgc
     2041 ggactctgca gggctggttg ttgccggtgt ttatgttgcc tatggcagta tatgcacaag
     2101 aggcaacggt gaaagaggtg catgacgcgc cagcggtgcg tggcagtatt atcgccaata
     2161 tgctgcagga gcatgacaat ccgttcacgc tctatcctta tgacaccaac tacctcatct
     2221 acacccaaac cagcgatctg aataaagaag cgattgccag ttacgactgg gcggaaaatg
     2281 cgcgtaagga tgaagtaaag tttcagttga gcctggcatt tccgctgtgg cgtgggattt
     2341 taggcccaaa ctcggtgttg ggtgcgtctt atacgcaaaa atcctggtgg caactgtcca
     2401 atagcgaaga gtcttcaccg tttcgtgaaa ccaactacga accgcaattg ttcctcggtt
     2461 ttgccaccga ttaccgtttt gcaggttgga cgctgcgcga tgtggagatg gggtataacc
     2521 acgactctaa cgggcgttca gacccgacct cccgcagctg gaaccgcctt tatactcgcc
     2581 tgatggcaga aaacggtaac tggctggtag aagtgaagcc gtggtatgtg gtgggtaata
     2641 ctgacgataa cccggatatc accaaatata tgggttacta ccagcttaaa atcggctatc
     2701 acctcggtga tgcggtgctc agtgcgaaag gacagtacaa ctggaacacc ggctacggcg
     2761 gcgcggagtt aggcttaagt tacccgatca ccaaacatgt gcgcctttat actcaggttt
     2821 acagcggcta tggcgaatcg ctcatcgact ataacttcaa ccagacccgt gtcggtgtag
     2881 gggttatgct aaacgacttg ttttgatgaa cggttgagtg gttggcaaat ctggaatcca
     2941 gcatccagga ttaccctctc agagactaaa agcattgcag tttctcgcgc aggcgctgaa
     3001 aatagcgcct gtttttattt caggcaatcg gggtgaatgt ggcgcaggcg gaagtgttga
     3061 atctggagtc cggagctaaa caggttttac aagaaacctt tggctaccaa cagtttcgcc
     3121 ccggccagga agaaattatc gacactgtgc tttccggtcg cgattgcctg gttgttatgc
     3181 ccactggtgg cggaaaatcc ctttgctatc aaattcctgc cttattgcta aacggcctta
     3241 ccgtggttgt ttcaccgctg atttcgttga tgaaagatca ggtggatcaa ctacaggcca
     3301 acggcgtggc ggcggcgtgc cttaactcga cgcaaacccg cgaacagcaa cttgaagtga
     3361 tgacaggctg ccgcaccggg caaattcgct tactgtatat cgccccggaa cgcctgatgc
     3421 tggataactt tcttgagcat ctggcgcact ggaatccggt gttattagcc gtcgatgaag
     3481 cgcactgtat ctcccaatgg ggtcacgatt tccgcccgga atatgccgcg ctcggtcagt
     3541 tgcgccagcg gttcccgacg ctgccgttta tggcgctgac cgccacagcc gacgacacca
     3601 cgcgccagga tatcgtgcgc ctgctggggc tgaacgatcc gctgattcaa atcagcagtt
     3661 ttgaccgtcc gaatattcgc tacatgctga tggagaaatt caaaccgctc gatcagttga
     3721 tgcgctacgt gcaggaacag cgcggtaagt ctggcattat ctactgcaac agccgggcga
     3781 aagtagaaga caccgctgcg cgcctgcaaa gcaggggtat tagcgcggcg gcctatcatg
     3841 ccgggctgga aaataatgtc cgcgccgacg tgcaggagaa attccagcgc gatgatctgc
     3901 aaattgtggt ggcgacggtg gcgttcggca tgggcatcaa taaaccaaac gttcgcttcg
     3961 tggtccactt tgatattccg cgcaatatcg aatcctatta tcaggaaacc ggtcgcgccg
     4021 ggcgtgatgg cctgcctgcg gaagcgatgc tgttttacga tccggctgat atggcgtggc
     4081 tgcgccgttg tctggaagag aagccgcagg ggcagttgca ggatatcgag cgccacaaac
     4141 tcaatgcgat gggcgcgttt gccgaagcgc aaacttgccg tcgtctggta ttgctgaact
     4201 attttggcga agggcgtcag gagccgtgcg ggaactgcga tatctgcctc gatccgccga
     4261 aacagtacga cggttcaacc gatgctcaga ttgccctttc caccattggt cgtgtgaatc
     4321 agcggtttgg gatgggttat gtggtggaag tgattcgtgg tgctaataac cagcgtatcc
     4381 gcgactatgg tcatgacaaa ctgaaagtct atggtatggg ccgtgataaa agccatgaac
     4441 attgggtgag cgtgatccgc cagctgattc atctcggcct ggtgacgcaa aatattgccc
     4501 agcattctgc cctacaactg acagaggccg cgcgcccggt gctgcgcggc gaatcctctt
     4561 tgcaacttgc cgtgccgcgt atcgtggcgc tcaaaccgaa agcgatgcag aaatcgttcg
     4621 gcggcaacta tgatcgcaaa ctgttcgcca aattacgcaa actgcgtaaa tcgattgccg
     4681 atgaaagtaa tgtcccgccg tacgtggtgt ttaacgacgc aaccttgatt gagatggctg
     4741 aacagatgcc gatcaccgcc agcgaaatgc tcagcgttaa cggcgttggg atgcgcaagc
     4801 tggaacgctt tggtaaaccg tttatggcgc tgattcgtgc gcatgtcgat ggcgacgacg
     4861 aagagtagtc agcagcataa aaaagtgcca gtatgaagac tccgtaaacg tttcccccgc
     4921 gagtcaaatg tatgttgatg ttatttctca ccgtcgccat ggtgcacatt gtggcgctta
     4981 tgagccccgg tcccgatttc ttttttgtct ctcagaccgc tgtcagtcgt tcccgtaaag
     5041 aagcgatgat gggcgtgctg ggcattacct gcggcgtaat ggtttgggct gggattgcgt
     5101 tgcttggcct gcatttgatt atcgaaaaaa tggcctggct gcatacgctg attatggtag
     5161 gcggtggcct gtatctctgc tggatgggtt accagatgct acgcggtgca ctgaaaaaag
     5221 aggctgtatc tgcgcctgca ccacaggttg agctggcgaa aagcgggcgc agtttcctga
     5281 aaggcttact gaccaatctc gccaatccga aagcgattat ctattttggt tcggtattct
     5341 cattgtttgt cggcgataac gttggcacta ccgagcgctg gggcattttc gcgttgatta
     5401 ttgtcgaaac actggcgtgg tttaccgtcg ttgccagtct gtttgccctg ccgcaaatgc
     5461 gccgtggtta tcaacgtctg gcgaagtgga ttgatggttt tgccggggcg ttatttgccg
     5521 gatttggcat tcatttgatt atttcgcggt gatgccagac gcgtcttcag agtaagtcgg
     5581 ataaggcgtt tacgccgcat ccgacattat ttttcacgca tgcctcgccg atgctaacag
     5641 cgctcccacc agcataaaca acgagccgaa aatcttattc agcgccttca tctgctttgg
     5701 tcctttaatc catagagcaa tcctttgagc aagggtggcg taaccgatca tcacaataat
     5761 atcgaccaca atagtggtga cgccgagcac gatatactgc atcagttgcg gctgttgcgg
     5821 catgatgaat tgcggaaata gcgccgccag aaacacaata cttttgggat tggtgagatt
     5881 cacaaaaact gcgcgctgga acaaatgtcg acgcgattga gtagaggcca gcgatttaag
     5941 atcaattgcg ccagcggcgc gccactgctg gattcccagc caaatcaagt aagccgcgcc
     6001 tgcccacttt aacacttcaa acgcaatcac tgagcgggaa aatagcgtcc ccaaccccac
     6061 gccaaccagc acaatatgaa tcgccagtcc ggtctgaagc ccagcaatag acgccaccgc
     6121 gccgcgataa ccgtggttga gcgaggtggt catagtgttg attgcaccag agcctggcga
     6181 caggcttaaa atgatcgatg tcagcaggta ggcaaaccac cattctaagg tcatgatgaa
     6241 ctcccggtgt gtctattttt gtgccacaat acgctactgt cgcagcgttg tgtcaggcac
     6301 gcttaaaaaa acgattttac gtggtttaag aggcagatta cccgatgttt cagcagcaaa
     6361 aagactggga aacaagagaa aacgcgtttg ctgcttttac catgggaccg ctgactgatt
     6421 tctggcgtca gcgtgatgaa gcagagttta ctggtgtgga tgacattccg gtgcgctttg
     6481 tccgttttcg cgcacagcac catgaccggg tggtagtcat ctgcccgggg cgtattgaga
     6541 gctacgtaaa atatgcggaa ctggcctacg acctgttcca tttggggttt gatgtcttaa
     6601 tcatcgacca tcgcgggcag ggacgttccg gtcgcctgtt agccgatccg catctcgggc
     6661 atgttaatcg ctttaatgat tatgttgatg atctggcggc attctggcag caggaggttc
     6721 agcccggtcc gtggcgtaaa cgctatatac tggcacattc gatgggcggt gcgatctcca
     6781 cattatttct gcaacgccat ccaggtgtat gtgacgccat tgcgctaact gcgccaatgt
     6841 ttgggatcgt gattcgtatg ccgtcattta tggcacggca gatcctcaac tgggccgaag
     6901 cgcatccacg tttccgtgat ggctatgcaa taggcaccgg gcgctggcgc gcgttgccgt
     6961 ttgctatcaa cgtactgacc cacagcagac agcgatatcg acgtaactta cgcttctatg
     7021 ctgatgaccc aacgattcgc gtcggtgggc cgacctacca ttgggtacgc gaaagtattc
     7081 tggctggcga acaggtgtta gccggtgcgg gtgatgacgc cacgcaaacg cttctcttac
     7141 aagctgaaga ggaacgcgtg gtggataacc gcatgcacga ccgtttttgt gaactccgca
     7201 ccgccgcggg ccatcctgtc gaaggaggac ggccgttggt aataaaaggt gcttaccatg
     7261 agatcctttt tgaaaaggac gcaatgcgct cagtcgcgct ccacgccatc gttgattttt
     7321 tcaacaggca taactcaccc agcggaaacc gctctacaga ggtttaaatt tcttatgtac
     7381 caggttgttg cgtctgattt agatggcacg ttactttctc ccgaccatac gttatcccct
     7441 tacgccaaag aaactctgaa gctgctcacc gcgcgcggca tcaactttgt gtttgcgacc
     7501 ggtcgtcacc acgttgatgt ggggcaaatt cgcgataatc tggagattaa gtcttacatg
     7561 attacctcca atggtgcgcg cgttcacgat ctggatggca atctgatttt tgctcataac
     7621 ctggatcgcg acattgccag cgatctgttt ggcgtagtca acgacaatcc ggacatcatt
     7681 actaacgttt atcgcgacga cgaatggttt atgaatcgcc atcgcccgga agagatgcgc
     7741 ttttttaaag aagcggtgtt caaatatgcg ctgtatgagc cgggattact ggagccggaa
     7801 ggcatcagca aagtgttttt cacctgcgat tcccatgaac aactgctgcc gctggagcag
     7861 gcgattaacg ctcgctgggg cgatcgcgtc aacgtcagtt tctctacctt aacctgtctg
     7921 gaagtgatgg cgggcggcgt gtcgaaaggc catgcgctgg aagcagtggc gaagaaactg
     7981 ggctacagcc tgaaggattg tattgcgttt ggtgacggga tgaacgacgc cgaaatgctg
     8041 tcgatggcgg ggaaaggctg cattatgggc agtgcgcacc agcgtctgaa agatctgcat
     8101 ccggagctgg aagttattgg tactaatgcc gacgacgcgg tgccgcatta tctgcgtaaa
     8161 ctctatttat cgtaatcgtt ctttatttgg tcagttgtca acctgatact tcgctacaat
     8221 ggatactcgt taatcaaaga gttttccatt gtggcgttac ttatcatcac cacgattctg
     8281 tgggccttct cctttagctt ttatggcgag taccttgcgg ggcacgtcga tagctatttt
     8341 gcggtgctgg tgcgcgttgg cttggcggca ctcgtttttc tgccgtttct gcgtacccgt
     8401 agcaatagcc tgaaaacggt cggcctgtat atgctggtgg gcgcgatgca gctcggcgtg
     8461 atgtatatgc tgagtttccg cgcttatctc tacctgacgg tctccgaatt gttgctgttc
     8521 accgtactga cgccgctcta catcacgctg atttatgaca tcatgagtaa gcgccgtctg
     8581 cgctggggct atgcttttag cgccttgctg gcggtgattg gtgccgggat tattcgctat
     8641 gatcaggtca ccgaccattt ctggactggc ttgttgctgg tgcaactctc caatatcact
     8701 tttgccatcg gcatggtggg ttacaaacgc ctgatggaaa ctcgcccgat gccgcagtat
     8761 aacgcctttg cgtggttcta tcttggcgcg ttcctggtgg cggtaattgc atggttcttg
     8821 ctgggaaatg cgcagaaaat gccgcaaacc acgttgcaat ggggcattct ggtgtttctt
     8881 ggcgtggtgg cttccgggat tggctacttt atgtggaact acggcgcgac gcaggtggac
     8941 gccggaacgc tgggcattat gaataatatg cacgttccgg cagggctgct ggtaaacctg
     9001 gctatctggc accaacagcc gcactggcca acgtttatta caggcgcgct ggtgatcctg
     9061 gcctcactgt gggtgcatcg taagtgggtc gctccgcgct cttcacaaac ggcagatgat
     9121 cgcaggcgtg attgcgcgct gagcgaataa acgcttccgt aactggctga cgctgctcgc
     9181 catcgcgcac ggcggcgtac agtcggctcc acaagccttc gcccagggtt ttggtcacca
     9241 ccagaccctg gcgctcaaaa ctctctacta cccaatgcgg tagcgcggca atacccatcc
     9301 gcgcggcaac catttgaatc aacaataagg tgttatcgac gcttttcagt gacgggctga
     9361 cgcctgccgg ctgaagaaaa tgccgccaga catccagtcg actacgctgc accggataaa
     9421 ttaatagcgt ctcgctggcg agatcttccg gtgtaatacg cgtttttgcc gccagtggat
     9481 ggtcaggtgc taacaccagg cgtacttcat aatcgaacat cggcgaataa tgcaggccac
     9541 tgcgcggcag gatatcggag gtcatcacca gatccagctc gccctgttgc aacgcgggct
     9601 gcgggtcaaa cgtcacgccc gatttaaaat ccatctctac ctgcggccag ttcttatgga
     9661 aatttcctaa agcgggtgtc agccactgaa tacagctatg gcactcaatg gcaatgcgca
     9721 gacgcgtctg ctgcggttca ttgcaggctt gcagtgcctg gctgatttgc ggcagcacct
     9781 ggtttgccag ttgcaacagg atttctccct gcggtgtaaa gcgtagcggc tggctcttac
     9841 gcacaaatag ccggaagcca aggcgttgtt ccagatcgct aaactggtga gacagggcgg
     9901 attgcgtctg atgcaacgtt gccgcagcgg ctgcgagcga gccgcagttc cgcaacgctt
     9961 gtagcgtttt caggtgtttt acttcgatca tgaaagtcct tcacttcggc atgaataatt
    10021 tgcgcttgag gaatatacag taaccgccaa ttatggatgt gtaaacatct ggacggctaa
    10081 aatcattcgt cttttaaatt tatggtgcgt tggctgcgtt tctccacccc ggtcacttac
    10141 ttcagtaagc tcccggcgat gaataaactt gccgccttcc ctaaattcaa aatccatagg
    10201 atttacatat aattagagga agaaaaatga caatattgaa tcacaccctc ggtttccctc
    10261 gcgttggcct gcgtcgcgag ctgaaaaaag cgcaagaaag ttattgggcg gggaactcca
    10321 cgcgtgaaga actgctgaca gtagggcgtg aactgcgtgc tcgtcactgg gatcaacaaa
    10381 agcaagcggg tatcgacctg ctgccggtgg gcgattttgc ctggtacgat catgtactga
    10441 ccaccagttt gctgctgggt aatgttccgc cacgtcatca gaacaaagac ggttcggtag
    10501 atatcgacac cctgttccgt attggtcgtg gacgtgcgcc gactggcgaa cctgcggcgg
    10561 cagcggaaat gaccaaatgg tttaacacca actatcacta catggtgccg gagttcgtta
    10621 aaggccaaca gttcaaactg acttggacgc agctgctgga ggaagtggac gaggcgctgg
    10681 cgctgagcca caacgttaag cctgtgctgc tggggccggt tacttacctg tggctgggga
    10741 aagtgaaagg tgaacagttt gaccgcctta gcctgctgaa cgacattctg ccggtttatc
    10801 agcaagtgct ggcagaactg gcgaaacgcg gcatcgagtg ggtacagatt gatgaacccg
    10861 cgctggtact ggaactacca caggcgtggc tggacgcata caaacccgct tacgacgcgc
    10921 tccagggaca ggtgaaactg ctgctgacca cctattttga aggcgtaacg ccaaatctcg
    10981 acacgattac tgcgctgcct gttcaggggc tgcatgtgga ccttgtacat ggtaaagata
    11041 acgttgttga actgcacaag cgcctgcctt ctgactggct gctgtctgcg ggtctgatca
    11101 atggtcgtaa cgtctggcgc gccgatctta ctgagaaata tgcgcaaatt aaggacattg
    11161 tcggcaaacg tgatttgtgg gtggcatctt cctgctcgtt gctgcacagc cccatcgacc
    11221 tgagcgtgga aacgcgtctt gatgcagaag tgaaaagctg gtttgccttc gccctacaaa
    11281 aatgccatga actggcactg ctgcgcgatg cgctgaacag tggtgacacg gcagctctgg
    11341 cagagtggag cgccccaatt caggcgcgtc gtcactctac tcgtgtacat aatccggcgg
    11401 tagaaaagcg tctggcggcg atcaccgccc aggacagcca gcgtgcgaat gtctatgaag
    11461 tgcgtgctga agcccagcgt gcgcgtttta aactgccagc gtggccgacc accacgattg
    11521 gttccttccc gcaaaccacg gaaattcgta ccctgcgtct ggatttcaaa aagggcaatc
    11581 tcgacgccaa caactaccgc acgggcattg cggaacatat caagcaggcc attgttgagc
    11641 aggaacgttt gggactggat gtgctggtac atggcgaggc cgagcgtaat gacatggtgg
    11701 aatactttgg cgagcacctc gacggatttg tctttacgca aaacggttgg gtacagagct
    11761 acggttcccg ctgcgtgaag ccaccgattg tcattggtga cattagccgc ccggcaccga
    11821 ttaccgtgga gtgggcgaag tatgcgcaat cgctgaccga caaaccggtg aaagggatgc
    11881 tgacggggcc ggtgaccata ctctgctggt cgttcccgcg tgaagatgtc agccgtgaaa
    11941 ccatcgccaa acagattgcg ctggcgctgc gtgatgaagt ggccgatctg gaagccgctg
    12001 gaattggcat catccagatt gacgaaccgg cgctgcgcga aggtttaccg ctgcgtcgta
    12061 gcgactggga tgcgtatctc cagtggggcg tagaggcctt ccgtatcaac gccgccgtgg
    12121 cgaaagatga cacacaaatc cacactcaca tgtgttattg cgagttcaac gacatcatgg
    12181 attcgattgc ggcgctggac gcagacgtca tcaccatcga aacctcgcgt tccgacatgg
    12241 agttgctgga gtcgtttgaa gagtttgatt atccaaatga aatcggtcct ggcgtctatg
    12301 acattcactc gccaaacgta ccgagcgtgg aatggattga agccttgctg aagaaagcgg
    12361 caaaacgcat tccggcagag cgcctgtggg tcaacccgga ctgtggcctg aaaacgcgcg
    12421 gctggccaga aacccgcgcg gcactggcga acatggtgca ggcggcgcag aatttgcgtc
    12481 gggggtaaat ccaaaccggg tggtaatacc acccggtctt ttctcattac agcgacttct
    12541 tcccaccata ctgcttaaac cattccagca tacgctgcca gccatctttc gcagattcgg
    12601 catgatagct cgggcgataa tcagcgttga atgcatgccc ggcatccggg tacacgataa
    12661 tctctgcttt cgcattagca gcccgcagcg cctggcgcat cgtttcaacg ctctcctgcg
    12721 gaatgctgtt atcctgacca ccatataagc cgagaaccgg cgcgttaaga tcggttgcga
    12781 tatcaacagg ttgtttcggt gaattcagcg acttgtcgcc cgtcagtttg ccgtaccacg
    12841 ccactgcggc ttttagctgt ggattatgcg cggcatacag ccaggtgata cgtccacccc
    12901 agcagaatcc ggtgatcatt aaacgatgaa catcgccgcc gttgcgggaa gcccaactgg
    12961 cgacatgatc gagatcggcc agcacctgcg agtcaggcac ttttgctacc agaccgctaa
    13021 gcaacgtggg gatatcggca aaatcattcg gatcgccttc gcggaagtaa agttcaggtg
    13081 cgatagccag atacccctcc agcgccagac ggcgacaaat gtcgcggata tgttcatgca
    13141 cgccaaaaat ttcctgcact acaatgacca ctggcagtgg gccatcgctt tgctttggtc
    13201 tggcatggta agcaggcatg ttatcccctt gtgaagggat agaggtgaag cccgccacaa
    13261 tcgcgtcgtc cggggtctga acgatggtcg aagcgagagg cgatgcagca ggtgcaaatc
    13321 cagattgttg tgttgttgcc atggtattct ccgtaccctt ataaaaatgt tgcgcaatgt
    13381 taactatagt cagcatgcaa caaatcacat tgcctgaatc ggctcatctt ttatgcagtc
    13441 ctgcagaatg aagagtgatt tatgtgatat gaatcacttt tggtgggtaa atttatgcaa
    13501 cgcatttgcg tcatggtgat gagtatcacg aaaaaatgtt aaacccttcg gtaaagtgtc
    13561 tttttgcttc ttctgactaa accgattcac agaggagttg tatatgtcca agtctgatgt
    13621 ttttcatctc ggcctcacta aaaacgattt acaaggggct acgcttgcca tcgtccctgg
    13681 cgacccggat cgtgtggaaa agatcgccgc gctgatggat aagccggtta agctggcatc
    13741 tcaccgcgaa ttcactacct ggcgtgcaga gctggatggt aaacctgtta tcgtctgctc
    13801 taccggtatc ggcggcccgt ctacctctat tgctgttgaa gagctggcac agctgggcat
    13861 tcgcaccttc ctgcgtatcg gtacaacggg cgctattcag ccgcatatta atgtgggtga
    13921 tgtcctggtt accacggcgt ctgtccgtct ggatggcgcg agcctgcact tcgcaccgct
    13981 ggaattcccg gctgtcgctg atttcgaatg tacgactgcg ctggttgaag ctgcgaaatc
    14041 tattggcgcg acaactcacg ttggcgtgac agtttcttct gataccttct acccaggtca
    14101 ggaacgttac gatacttact ctggtcgcgt agttcgtcac tttaaaggtt ctatggaaga
    14161 gtggcaggcg atgggcgtaa tgaactatga aatggaatct gcaactctgc tgaccatgtg
    14221 tgcaagtcag ggcctgcgtg ccggtatggt agcgggtgtt atcgttaacc gcacccagca
    14281 agagatcccg aatgctgaga cgatgaaaca aaccgaaagc catgcggtga aaatcgtggt
    14341 ggaagcggcg cgtcgtctgc tgtaattctc ttctcctgtc tgaaggccga cgcgttcggc
    14401 cttttgtatt tttgcgtagc gcctcgcagg aaatgccttt ccaactggac gtttgtacag
    14461 cacaattcta ttttgtgcgg gtaagttgtt gcgtcaggag gcgttgtgga tttctcaatc
    14521 atggtttacg cagttattgc gttggtgggt gtggcaattg gctggctgtt tgccagttat
    14581 caacatgcgc agcaaaaagc cgagcaatta gctgaacgtg aagagatggt cgcggagtta
    14641 agcgcggcaa aacaacaaat tacccaaagc gagcactggc gtgcagagtg tgaattgctc
    14701 aataacgaag tgcgcagcct gcaaagtatt aacacctctc tggaggccga tctgcgtgaa
    14761 gtaaccacgc ggatggaagc cgcacagcaa catgctgacg ataaaattcg ccagatgatt
    14821 aacagcgagc agcgcctcag tgagcagttt gaaaacctcg ccaaccgtat ttttgagcac
    14881 agcaatcgcc gggttgatga gcaaaaccgt cagagtctga acagcctgtt gtcgccgcta
    14941 cgtgaacaac tggacggttt ccgccgtcag gttcaggaca gcttcggtaa agaagcacaa
    15001 gaacgccata ccctgaccca cgaaattcgc aatctccagc aactcaacgc gcaaatggcc
    15061 caggaagcga tcaacctgac gcgcgcgctg aaaggcgaca ataaaaccca gggcaactgg
    15121 ggcgaggtag tattgacgcg ggtgctggag gcttccggtc tgcgtgaagg gtatgaatat
    15181 gaaacccagg tcagcatcga aaatgacgcc cgctcgcgga tgcagccgga tgtcatcgtg
    15241 cggctgccgc agggaaaaga tgtggtgatc gacgccaaaa tgacgctggt cgcctatgaa
    15301 cgctatttta acgccgaaga cgactacacc cgcgaaagcg cgctacagga acatatcgcg
    15361 tcggtgcgta accatatccg tttgctggga cgcaaagatt atcaacagct gccggggctg
    15421 cgaactctgg attacgtgct gatgtttatt cccgttgaac ccgctttttt actggcgctt
    15481 gaccgccagc cggagctgat caccgaagcg ttgaaaaaca atatcatgct ggttagcccg
    15541 actacgctgc tggtggcgtt gcgtactatt gccaacctgt ggcgttatga gcatcaaagc
    15601 cgtaacgccc agcaaatcgc cgatcgtgcc agcaagctgt acgacaagat gcgtttgttc
    15661 atcgatgaca tgtccgcgat tggtcaaagt ctcgacaaag cgcaggataa ctaccgccag
    15721 gcaatgaaaa aactctcttc aggtcgcgga aatgtgctgg cgcaggcaga agcgtttcgc
    15781 ggtttaggag tagaaattaa acgcgagatt aatccggatt tggctgaaca ggcggtgagc
    15841 caggatgaag agtatcgact tcggtcggtt ccggagcagc cgaatgatga agcttatcaa
    15901 cgcgatgatg aatataatca gcagtcgcgc tagcccatta ggggtagtta agccgggtag
    15961 aaatctaggg catcgacgcc caatctgtta cacttctgga acaatttttt gatgagcagg
    16021 cattgagatg gtggataagt cacaagaaac gacgcacttt ggttttcaga ccgtcgcgaa
    16081 ggaacaaaaa gcggatatgg tcgcccacgt tttccattcc gtggcatcaa aatacgatgt
    16141 catgaatgat ttgatgtcat ttggtattca tcgtttgtgg aagcgattca cgattgattg
    16201 cagcggcgta cgccgtgggc agaccgtgct ggatctggct ggtggcaccg gcgacctgac
    16261 agcgaaattc tcccgcctgg tcggagaaac tggcaaagtg gtccttgctg atatcaatga
    16321 atccatgctc aaaatgggac gcgagaagct gcgtaatatc ggtgtgattg gcaacgttga
    16381 gtatgttcag gcgaacgctg aggcgctgcc gttcccggat aacacctttg attgcatcac
    16441 catttcgttt ggtctgcgta acgtcaccga caaagataaa gcactgcgtt caatgtatcg
    16501 cgtgctgaaa cccggcggcc gcctgctggt gcttgagttc tcgaagccaa ttatcgagcc
    16561 gctgagcaaa gcctatgatg catactcctt ccatgtgctg ccgcgtattg gctcactggt
    16621 cgcgaacgac gccgacagct accgttatct ggcagaatcc atccgtatgc atcccgatca
    16681 ggataccctg aaagccatga tgcaggatgc cggattcgaa agtgtcgact actacaatct
    16741 gacggcaggg gttgtggcgc tgcatcgtgg ttataagttc tgacaggaga ccggaaatgc
    16801 cttttaaacc tttagtgacg gcaggaattg aaagtctgct caacaccttc ctgtatcgct
    16861 cacccgcgct gaaaacagcc cgctcgcgtc tgctgggtaa agtattgcgc gtggaggtaa
    16921 aaggcttttc gacgtcattg attctggtgt tcagcgaacg ccaggttgat gtactgggcg
    16981 aatgggcagg cgatgctgac tgcaccgtta tcgcctacgc cagtgtgttg ccgaaacttc
    17041 gcgatcgcca gcagcttacc gcactgattc gcagtggtga gctggaagtg cagggcgata
    17101 ttcaggtggt gcaaaacttc gttgcgctgg cagatctggc agagttcgac cctgcggaac
    17161 tgctggcccc ttataccggt gatatcgccg ctgaaggaat cagcaaagcc atgcgcggag
    17221 gcgcaaagtt cctgcatcac ggcattaagc gccagcaacg ttatgtggcg gaagccatta
    17281 ctgaagagtg gcgtatggca cccggtccgc ttgaagtggc ctggtttgcg gaagagacgg
    17341 ctgccgtcga gcgtgctgtt gatgccctga ccaaacggct ggaaaaactg gaggctaaat
    17401 gacgccaggt gaagtacggc gcctatattt catcattcgc acttttttaa gctacggact
    17461 tgatgaactg atccccaaaa tgcgtatcac cctgccgcta cggctatggc gatactcatt
    17521 attctggatg ccaaatcggc ataaagacaa acttttaggt gagcgactac gactggccct
    17581 gcaagaactg gggccggttt ggatcaagtt cgggcaaatg ttatcaaccc gccgcgatct
    17641 ttttccaccg catattgccg atcagctggc gttattgcag gacaaagttg ctccgtttga
    17701 tggcaagctg gcgaagcagc agattgaagc tgcaatgggc ggcttgccgg tagaagcgtg
    17761 gtttgacgat tttgaaatca agccgctggc ttctgcttct atcgcccagg ttcataccgc
    17821 gcgattgaaa tcgaatggta aagaggtggt gattaaagtc atccgcccgg atattttgcc
    17881 ggtcattaaa gcggatctga aacttatcta ccgtctggct cgctgggtgc cgcgtttgct
    17941 gccggatggt cgccgtctgc gcccaaccga agtggtgcgc gagtacgaaa agaccttgat
    18001 tgatgaactg aatttgctgc gggaatctgc caacgccatt cagcttcggc gcaattttga
    18061 agacagcccg atgctctaca tcccggaagt ttaccctgac tattgtagtg aagggatgat
    18121 ggtgatggag cgcatttacg gcattccggt gtctgatgtt gcggcgctgg agaaaaacgg
    18181 cactaacatg aaattgctgg cggaacgcgg cgtgcaggtg ttcttcactc aggtctttcg
    18241 cgacagcttt ttccatgccg atatgcaccc tggcaacatc ttcgtaagct atgaacaccc
    18301 ggaaaacccg aaatatatcg gcattgattg cgggattgtt ggctcgctaa acaaagaaga
    18361 taaacgctat ctggcagaaa actttatcgc cttctttaat cgcgactatc gcaaagtggc
    18421 agagctacac gtcgattctg gctgggtgcc accagatacc aacgttgaag agttcgaatt
    18481 tgccattcgt acggtctgtg aacctatctt tgagaaaccg ctggccgaaa tttcgtttgg
    18541 acatgtactg ttaaatctgt ttaatacggc gcgtcgcttc aatatggaag tgcagccgca
    18601 actggtgtta ctccagaaaa ccctgctcta tgtcgaaggg gtaggacgcc agctttatcc
    18661 gcagctcgat ttatggaaaa cagcgaagcc tttcctggag tcgtggatta aagatcaggt
    18721 cggtattcct gctctggtga gagcatttaa agaaaaagcg ccgttctggg tcgaaaaaat
    18781 gccagaactg cctgaactgg tttacgacag tttgcgccag ggcaagtatt tacagcacag
    18841 tgttgataag attgcccgcg agcttcagtc aaatcatgta cgtcagggac aatcgcgtta
    18901 ttttctcgga attggcgcta cgttagtatt aagtggcaca ttcttgttgg tcagccgacc
    18961 tgagtggggg ctgatgcccg gctggttaat ggcaggtggt ctgatcgcct ggtttgtcgg
    19021 ttggcgcaaa acacgctgat tttttcatcg ctcaaggcgg gccgtgtaac gtataatgcg
    19081 gctttgttta atcatcatct accacagagg aacatgtatg ggtggtatca gtatttggca
    19141 gttattgatt attgccgtca tcgttgtact gctttttggc accaaaaagc tcggctccat
    19201 cggttccgat cttggtgcgt cgatcaaagg ctttaaaaaa gcaatgagcg atgatgaacc
    19261 aaagcaggat aaaaccagtc aggatgctga ttttactgcg aaaactatcg ccgataagca
    19321 ggcggatacg aatcaggaac aggctaaaac agaagacgcg aagcgccacg ataaagagca
    19381 ggtgtaatcc gtgtttgata tcggttttag cgaactgcta ttggtgttca tcatcggcct
    19441 cgtcgttctg gggccgcaac gactgcctgt ggcggtaaaa acggtagcgg gctggattcg
    19501 cgcgttgcgt tcactggcga caacggtgca gaacgaactg acccaggagt taaaactcca
    19561 ggagtttcag gacagtctga aaaaggttga aaaggcgagc ctcactaacc tgacgcccga
    19621 actgaaagcg tcgatggatg aactacgcca ggccgcggag tcgatgaagc gttcctacgt
    19681 tgcaaacgat cctgaaaagg cgagcgatga agcgcacacc atccataacc cggtggtgaa
    19741 agataatgaa gctgcgcatg agggcgtaac gcctgccgct gcacaaacgc aggccagttc
    19801 gccggaacag aagccagaaa ccacgccaga gccggtggta aaacctgctg cggacgctga
    19861 accgaaaacc gctgcacctt ccccttcgtc gagtgataaa ccgtaaacat gtctgtagaa
    19921 gatactcaac cgcttatcac gcatctgatt gagctgcgta agcgtctgct gaactgcatt
    19981 atcgcggtga tcgtgatatt cctgtgtctg gtctatttcg ccaatgacat ctatcacttg
    20041 gtatccgcgc cattgatcaa gcagttgccg caaggttcaa cgatgatcgc caccgacgtg
    20101 gcctcgccgt tctttacgcc gatcaagctg acctttatgg tgtcgctgat tctgtcagcg
    20161 ccggtgattc tctatcaggt gtgggcattt atcgccccag cgctgtataa gcatgaacgt
    20221 cgcctggtgg tgccgctgct ggtttccagc tctctgctgt tttatatcgg catggcattc
    20281 gcctactttg tggtctttcc gctggcattt ggcttccttg ccaataccgc gccggaaggg
    20341 gtgcaggtat ccaccgacat cgccagctat ttaagcttcg ttatggcgct gtttatggcg
    20401 tttggtgtct cctttgaagt gccggtggca attgtgctgc tgtgctggat ggggattacc
    20461 tcgtcagaag acttacgcaa aaaacgcccg tatgtgctgg ttggtgcatt cgttgtcggg
    20521 atgttgctga cgccgccgga tgtcttctcg caaacgctat tggcgatccc gatgtactgt
    20581 ctgtttgaaa tcggtgtctt cttctcacgc ttttacgttg gtaaagggcg aaatcgggaa
    20641 gaggaaaacg acgctgaagc agaaagcgaa aaaactgaag aataaattca accgcccgtc
    20701 agggcggttg tcatatggag tacaggatgt ttgatatcgg cgttaatttg accagttcgc
    20761 aatttgcgaa agaccgtgat gatgttgtag cgcgcgcttt tgacgcggga gttaatgggc
    20821 tactcatcac cggcactaac ctgcgtgaaa gccagcaggc gcaaaagctg gcgcgtcagt
    20881 attcgtcctg ttggtcaacg gcgggcgtac atcctcacga cagcagccag tggcaagctg
    20941 cgactgaaga agcgattatt gagctggccg cgcagccaga agtggtggcg attggtgaat
    21001 gtggtctcga ctttaaccgt aacttttcga cgccggaaga gcaggaacgc gcttttgttg
    21061 ctcagctacg cattgccgca gaattaaaca tgccggtatt tatgcactgt cgcgatgccc
    21121 acgagcggtt tatgacattg ctggagccgt ggctggataa actgcctggt gcggttcttc
    21181 attgctttac cggcacacgc gaagagatgc aggcgtgcgt ggcgcgtgga atttatatcg
    21241 gcattaccgg ttgggtttgc gatgaacgac gcgggctgga gctgcgggaa ttgttgccgt
    21301 tgattccggc ggagaaattg ctgatcgaaa cagatgcgcc gtatctgctc ccgcgcgatc
    21361 tcacgccaaa gccatcatcc cggcgcaacg agccagccca tctgccccat attttgcaac
    21421 gtattgcgca ctggcgtgga gaagatgccg catggctggc tgccaccacg gatgccaatg
    21481 tcaaaacact gtttgggatt gcgttttaga gtttgcggaa ctcggtgttc ttcacactgt
    21541 gcttaatctc tttattaata agattaagca atagcatgga gcgagcctca ccatcgggtt
    21601 cggtgaaaat ggcctgaaag ccttcgaacg cgccttcggt aataatcacc ttatcacccg
    21661 gataaggggg ggccggatcg acaatgtctt tcggtttata taccgatagc tgatgaatta
    21721 ccgccgatgg gactatcgct ggcgacgcgc caaagcgcac aaagtggctg acgccacgcg
    21781 tagcgctgat ggtcgtggta tgaatcactt ctggatcaaa ttccacaaac aggtagttag
    21841 ggaataacgg ttcactgact gcggtacgtt taccccgtac gattttttcc agggtgatca
    21901 tcggagcgag gcaattaacc gcctgtctct caaggtgttc ctgggcacgt tgaagttgcc
    21961 cgcgcttgca gtacagtaaa taccaggatt gcataatgac tcttatccgt ttaatcgggg
    22021 cgcaaggata gcaaaagctt tacgctaagt taattatatt ccccggtttg cgttataccg
    22081 tcagagttca cgctaattta acaaatttac agcattgcaa agatgaacgc cgtataatgg
    22141 gcgcagatta agaggctaca atggacgcca tgaaatataa cgatttacgc gacttcctga
    22201 cgctgcttga acagcagggt gagctaaaac gtatcacgct cccggtggat ccgcatctgg
    22261 aaatcactga aattgctgac cgcactctgc gtgctggtgg gcctgcgctg ttgttcgaaa
    22321 accctaaagg ctactcaatg ccggtgctgt gcaacctgtt cggtacgcca aagcgcgtgg
    22381 cgatgggcat ggggcaggaa gatgtttcgg cgctgcgtga agttggtaaa ttattggcgt
    22441 ttctgaaaga gccggagccg ccaaaaggtt tccgcgacct gtttgataaa ctgccgcagt
    22501 ttaagcaggt gttaaacatg ccgacaaagc gactgcgtgg tgcgccctgc caacaaaaaa
    22561 tcgtctctgg cgatgacgtc gatctcaatc gcattcccat tatgacctgc tggccggaag
    22621 atgccgcgcc gctgattacc tgggggctga ccgtaacgcg cggcccgcat aaagagcggc
    22681 agaatctggg catttatcgc cagcagctga ttggtaaaaa caaactgatt atgcgctggc
    22741 tgtcgcatcg cggcggcgcg ctggattatc aggagtggtg tgcggcgcat ccgggcgaac
    22801 gtttcccggt ttctgtggcg ctgggtgccg atcctgccac gattctcggt gcagtcaccc
    22861 ccgttccgga tacgctttca gagtatgcgt ttgccggatt gctacgcggc accaaaaccg
    22921 aagtagtaaa gtgtatttcc aatgacctcg aagtgccagc cagtgccgaa atcgtcctgg
    22981 aagggtatat cgatcctggt gagatggcgc cggaagggcc gtatggtgac cacacaggtt
    23041 actataatga agtcgataat ttcccggtgt ttaccgtgac gcatattacc cagcgtgaag
    23101 atgcgattta ccattccacc tataccgggc gtccgccaga tgagcccgcg gtactgggcg
    23161 tggcgttgaa cgaagtgttt gtaccgattc tgcaaaaaca gttcccggaa attgtcgatt
    23221 tttacctgcc gccggaaggc tgttcttatc gtctggcggt agtgacgatc aaaaaacagt
    23281 acgccggaca cgcgaagcgc gtcatgatgg gcgtctggtc gttcttacgc cagtttatgt
    23341 acactaaatt tgtgatcgtt tgcgatgatg acgtcaacgc acgcgactgg aacgatgtga
    23401 tttgggcgat taccacccgt atggacccgg cgcgggatac tgttctggta gaaaatacgc
    23461 ctattgatta tctggatttt gcctcgcctg tctctgggct gggttcaaaa atggggctgg
    23521 atgccacgaa taaatggccg ggggaaaccc agcgtgaatg gggacgtccc atcaaaaaag
    23581 atccagatgt tgtcgcgcat attgacgcca tctgggatga actggctatt tttaacaacg
    23641 gtaaaagcgc ctgatgcgcg tttgttttgc cctatttatc gatccgacag agaaagcgca
    23701 tgacaacctt aagctgtaaa gtgacctcgg tagcagctat cacggatacc gtatatcgtg
    23761 tccgcatcgt gccagacgcg gccttttctt ttcgtgctgg tcagtatttg atggtagtga
    23821 tggatgagcg cgacaaacgt ccgttctcaa tggcctcgac gccggatgaa aaagggttta
    23881 tcgagctgca tattggcgct tctgaaatca acctttacgc gaaagcagtc atggaccgca
    23941 tcctcaaaga tcatcaaatc gtggtcgacc ttccccacgg agaagcgtgg ctgcgcgatg
    24001 atgaagagcg tccgatgatt ttgattgcgg gcggcaccgg gttctcttat gcccgctcga
    24061 ttttgctgac agcgttggcg cgtaacccaa accgtgatat caccatttac tggggcgggc
    24121 gtgaagagca gcatctgtat gatctctgcg agcttgaggc gctttcgtta aagcaccttg
    24181 gtctgcaagt ggtgccggtg gttgaacaac cggaagcagg ctggcgtggg cgtactggta
    24241 ccgtgttaac ggcggtattg caggatcatg gtacgctggc agagcatgat atctatattg
    24301 ccggacgttt tgagatggcg aaaattgccc gtgacctgtt ttgcagtgag cgtaatgcgc
    24361 gggaagatcg cctgtttggc gatgcgtttg catttatctg agatataaaa aaacccgccc
    24421 ctgacaggcg ggaagaacgg caactaaaac tttattcagt ggcatttaga tctatgtcgc
    24481 atccggcaat tggtgcacga tgcctgatgc gacgctggcg cgtcttatca ggcctacatt
    24541 ggtgccggat cggtagaccg gataaggcgt tcacgccgca tccggcaagt ggttaaaccc
    24601 gctcaaacac cgtcgcaata ccctgaccca gaccgataca catcgtcgcc agaccaaact
    24661 gaacgtcttt gcgttccatc agattcagca gcgtggtgct gatacgcgca ccggaacaac
    24721 ccagcggatg acccagcgcg atcgcaccac cattgaggtt gatcttctcg tcaatctgct
    24781 ccattagccc gagatcttta atgcacggca ggatctgcgc ggcaaaggct tcgttcatct
    24841 caaacacgcc gatatcgctg gcagaaagcc ccgctttttt cagcgccagc ttcgaggccg
    24901 gaaccgggcc gtaacccata atcgatgggt cacaaccaac gaccgccatc gaacgcacac
    24961 gagcgcgcgg cttaagacct aattcatggg cgcggctttc actcatcacc agcatggcag
    25021 ctgcgccatc ggaaagtgca gaagatgtgc ccgccgttac cgtaccgttt actggatcaa
    25081 acgccggacg cagcgtggcg agggcttcca cggtggtttc cgggcgaatc acttcgtcgt
    25141 aattaaactg cttcaggacg ccgtcggcat cgtgaccacc agttgggatg atttcatttt
    25201 taaatgcggc cgactgcgtg gcggcccagg cgcgggcgtg tgaccgcgcg gcaaaggcat
    25261 cctgcatttc acggctgata ccgtgcatac gcgccagcat ttctgccgtt aagcccatca
    25321 tgcccgccgc tttggcgaca ttgcggctca ggccggggtg aaaatcgacg ccgtgactca
    25381 tcggcacatg gcctatatgc tccacgccgc caaccagaca tgcctgcgta tcgccagtca
    25441 tgatcattcg tgctgcgtca tgcagtgcct gcatggatga accacacaag cgattgacgg
    25501 taaccgccgg gacagagtgc ggcacttctg ccagcagcgc cgcgttacgg gcgatgttaa
    25561 aaccctgctc cagcgtctgt tgcacacaac cccagtaaat atcgtcgagg gccgccgctt
    25621 ccagcgccgg gttacgcgcc agcaggctac gcattaaatg agcggagaga tcttccgcac
    25681 gcacgttacg aaaagcaccg cccttcgaac ggcccatcgg ggtgcgaatt gcatcgacaa
    25741 tgacaacctg ttccattgtg actccttaag ccgttttcag gtcgccaacc ggacgggctg
    25801 gctcaaccgg aggatagtac ggttcgttat gacgcgcttt attacgcaga ccttccggca
    25861 cttcatacag cgggccgagg tgctgatatt gctgtgccat atcgaggtat tttgcgctac
    25921 cgagggtgtc cagccagcgg aacgcgccgc cgtggaacgg agggaagccc aggccgtaga
    25981 ccagcgccat atccgcttcc gccggagtgg cgataatgcc ctcttccaga cagcgcacca
    26041 cttcgttgac catcgggatc atcatgcggg cgataatctc ttcttcgctg aaatcgcgct
    26101 tcggttggct cacttctgcc agcaggtctt caacggcggc gtcttcttct ttcttcggct
    26161 tacctttgct gtcttcttta taacgccaga aaccgaggcc gttcttctga ccaaagcggt
    26221 tggcatcaaa cagcgcgtcg atggcatcgc ggtaatcttt ctgcatccgc tgcgggaagc
    26281 ctgctgccat gacagcctga gcgtgatgcg cggtatcaat gcccacaacg tccagcagat
    26341 atgccgggcc catcggccag ccaaactgtt tttccatcac tttgtcgatc ttgcggaaat
    26401 ccgcgccgtc gcgcagcagc tggctgaaac cggcgaaata cgggaacagc acgcggttaa
    26461 caaagaagcc ggggcagtcg ttaaccacaa tcggcgtctt gcccatcttg ctcgcccagg
    26521 cgacaacttt cgcgatggtt tcgtcggagc ttttctcgcc gcgaataatt tctaccaacg
    26581 gcattcggtg gaccgggtta aagaagtgca tcccgcagaa gttttccggg cgttccagcg
    26641 cgttggccag ttcgctgata ggaatggttg aagtgttaga cgccagcacg gtatcctggc
    26701 gtactttttg ttcggtttct gccagtacgg cttttttcac tttcgggttt tcaacaaccg
    26761 cttctaccac aatatccacg cggtcaaatc cggcgtagtc gagcgttggg tggatcgtgg
    26821 agatcacgcc agccagtttc agaccatcga ttttgccgcg ctcaagctgc ttgttcagca
    26881 gtttcgcggc ttcggtcatg ccgagggtta acgacttgtc gttgatatct ttcatgacaa
    26941 ccggcacgcc tttccacgcg gactggtaag cgataccgcc acccatgatg cctgcgccca
    27001 gcacagcagc ctgtttcggg gtttcaacgt ctttggtgag tttcttcgct ttgcctttta
    27061 catattgatc gttaaggaaa atgccgacca gtgcgcgagc ttcgttggta tgcgccagcg
    27121 ggacaaaact tttgttttcc agatttaagg cttcttcacg accaaaacgg gccgcagctt
    27181 caatggtttt tactgcggtg atgggggccg gataatgttt ccccgccgtt tgcgcgacca
    27241 ttcctttagc gatggtgaag ctcatggtgg cttcaatctt gctcagtttt agcggttcct
    27301 gcttcggctg acgtttcgct ttccagtcga ggtcgccgtt aatggcctgg cgtaaaatcg
    27361 ccatcgcccc ttcaatcagt ttttctgctt tgactacgcc atccaccaga ccgattttca
    27421 gcgcctgatc cgcgccgaca tctttaccgg cggcaatgat ttccagtgca ctgtcagcgc
    27481 ccagcatacg tggcatacgt acagaaccgc caaagcccgg catgatgccc agtttggttt
    27541 ccggcagacc gatgcgcaga tccggcgtcg ccagacgata atcggtcgcc agcacgcatt
    27601 cgcagccacc acccagcgca tagccgttga cggcagcaat ggtcggcacc ggcagatctt
    27661 ccaggcgatt aaacacgcta ttggcaaagt gcagccactg acttaactgt tcttcaggaa
    27721 cgaggaacag ggacaaaaat tcggtgatat cagcaccgac gataaaggct gctttgttcg
    27781 aacgcagcag cagccctttt agatctgatt gctgttccag cacgccgatg gcctcgccga
    27841 ggctggcgac ggtcgcagtg tcgagtttat taactgaacc tggggcatca aataccagtt
    27901 cggcaatgcc atcttccagc cagtcaaggt acagggtgtc gcctttgtaa agcatgtcag
    27961 tctcctgaat ccgcaaggta atctggtcgt accagatgca tcggagtgtg tgttttgtgt
    28021 taaaaatatg caaataaaag attaaagaaa tgccgatctg atcacgctcg gcagaaatca
    28081 cgctctggat gaacgatgtg ctaagatgcg gagacttaag gtcaaaaaaa cagaagggta
    28141 aaaaatggaa tcactggcct cgctctataa aaatcatata gctaccttac aagaacggac
    28201 tcgcgatgcg ctggcgcgct tcaagctgga tgcattactt attcactccg gcgagctgtt
    28261 caacgttttt ctcgacgatc atccctatcc gtttaaagtg aacccgcaat tcaaagcgtg
    28321 ggtgccggta actcaggtgc caaactgctg gttgctggtg gatggcgtga acaagccgaa
    28381 actgtggttc tatctgccgg ttgattactg gcacaacgtc gaaccgctgc cgacctcctt
    28441 ctggactgaa gatgtggaag tgatcgcgct gccgaaagcc gatggcattg gtagcctgct
    28501 gcctgctgcg cgcggcaata tcggttatat cggtccggtg ccggaacgtg cgctgcaact
    28561 gggtattgag gccagcaaca tcaacccgaa aggggtgatc gactacctgc attattaccg
    28621 ctccttcaaa accgagtacg aactggcctg tatgcgtgaa gcgcagaaaa tggcggtcaa
    28681 tggtcatcgt gcggcagaag aagcgttccg ttctggcatg agcgagttcg atatcaatat
    28741 tgcctatctg accgcgaccg gtcatcgtga taccgacgta ccttacagca acattgtggc
    28801 gcttaacgaa cacgctgcgg tgttgcatta caccaaactg gaccaccagg caccggaaga
    28861 gatgcgcagc ttcctgctgg atgccggggc cgaatataac ggctatgcgg ctgacctgac
    28921 ccgtacctgg tcggcaaaaa gcgacaacga ctacgcacag ctggtgaaag acgtaaatga
    28981 tgaacaactg gcgctgatcg ccaccatgaa agcaggcgtc agctatgtgg attaccacat
    29041 ccagttccat cagcgcatcg ccaaactgct gcgtaaacat caaatcatca ccgatatgag
    29101 tgaagaagcg atggtcgaaa acgatctcac cggaccgttt atgccgcacg gtattggcca
    29161 tccgctgggc ctgcaggtgc atgacgtcgc cggttttatg caggatgata gcggtacaca
    29221 cctcgcggca ccggcaaaat atccgtacct gcgctacacc cgtattctcc agccgggcat
    29281 ggtgttaacc atcgaaccgg gtatctactt cattgaatcg cttctggcac cgtggcgtga
    29341 agggcagttc agcaagcact tcaactggca gaaaattgaa gcactgaaac cgttcggcgg
    29401 cattcgtatc gaagacaacg tggtgatcca cgaaaacaac gtggaaaaca tgacccggga
    29461 tctgaaactg gcgtgatgga aagctggtta attcctgcgg caccggtcac ggtcgttgaa
    29521 gagatcaaaa agagccgctt cataacgatg ttggcgcata ccgatggcgt tgaggcggcg
    29581 aaagcgtttg ttgaatcggt gcgggcagaa caccccgatg cccgccacca ttgcgtggcg
    29641 tgggtggcgg gtgcgccgga tgattctcaa cagctgggtt tctctgacga cggggagccg
    29701 gcgggaacgg caggtaaacc gatgctcgcc cagctaatgg gcagcggcgt cggggaaatt
    29761 accgctgtgg tagtgcgcta ctacggcggc atattgcttg gcaccggtgg gttagtgaaa
    29821 gcgtatggcg gcggcgtgaa tcaggcgctg cgccagctaa cgacccaacg caagacgcca
    29881 ttaaccgaat atactttgca atgtgaatat agtcagttaa ccggcattga agcgttgctg
    29941 gggcagtgtg acggcaaaat tatcaacagt gattatcagg cattcgttct gctgcgggtg
    30001 gcgcttccgg cggcgaaagt ggctgaattt tccgcaaagc tggcggattt tagccgtggt
    30061 tcattgcaat tgttagcgat tgaagaataa tccccacttc gttttgcaga actaaggaag
    30121 cggcagagat gcattttcgc gccattaccc gaatcgttgg actactggtc atcttatttt
    30181 cagggaccat gattatccct gggctggtag cactcatcta ccgggatgga gcgggccgcg
    30241 cttttaccca gacctttttt gtcgccctcg ccattggctc tatgctgtgg tggccgaacc
    30301 gcaaagagaa gggcgaactg aaatcccgtg aggggtttct gatagtggtg ctgttttgga
    30361 ccgtgctggg tagtgtcggt gcgctccctt ttatcttctc ggaaagcccg aacctcacga
    30421 ttaccgatgc gttttttgaa tctttctctg gcctgaccac caccggggcc actacgctgg
    30481 tggggctgga ttcgctccct catgccatcc tcttttatcg ccagatgctg caatggtttg
    30541 gcgggatggg gatcatcgtg ttagcggttg cgatactgcc tatcctcggc gtgggtggga
    30601 tgcagctcta tcgcgcagaa atgcccggcc cgctgaaaga taacaaaatg cgcccgcgaa
    30661 ttgcggaaac ggcgaaaact ctgtggttga tttatgtctt gctgaccgtc gcctgtgcgc
    30721 tggcgttgtg gtttgccgga atggatgcct ttgatgccat cggccatagc tttgcgacta
    30781 tcgctattgg cggcttctcg acacatgatg ccagtatcgg ttatttcgac agtccgacta
    30841 ttaacactat cattgctatc ttcctgctga tctccggctg taactacggt ctgcactttt
    30901 cactgttaag tgggcgtagt ctgaaggttt attggcgcga tccggaattt cgcatgttta
    30961 tcggcgtaca gtttacgctg gtggttattt gtacactcgt actgtggttt cataatgtct
    31021 acagttcggc gctgatgaca attaaccagg cgtttttcca ggtggtatcg atggcgacaa
    31081 ccgccgggtt tacgactgac agcattgccc gctggccgct ctttttgccg gtactgcttt
    31141 tatgttcagc ttttatcggc ggttgcgccg ggtcaacggg cggtggcctg aaagtgatcc
    31201 gcatcctgct gctgtttaag caggggaacc gtgagctgaa acgactggtg catccgaacg
    31261 ccgtctatag cattaagctg gggaatcgcg cactgccgga acgtatcctc gaagccgttt
    31321 ggggattttt ctccgcctat gcattggtgt ttattgtcag tatgctggcg attatcgcca
    31381 cgggcgtgga tgatttttct gcctttgcct cagttgttgc gacattgaat aacctggggc
    31441 cagggcttgg cgtggttgct gataacttta ccagtatgaa cccggtggct aaatggatcc
    31501 tgattgccaa catgctgttt ggtcgtctcg aggtctttac attactggtg ctctttaccc
    31561 cgactttctg gcgtgaatga tggagtaata cgtgaaaaca ttaattcttt tctcaacaag
    31621 ggacggacaa acgcgcgaga ttgcctccta cctggcttcg gaactgaaag aactggggat
    31681 ccagacggat gtcgccaatg tgcaccgcat tgaagaacca caatgggaaa actatgaccg
    31741 tgtggtcatt ggtgcttcta ttcgctatgg tcactaccac tctgcgttcc aggaatttgt
    31801 caaaaaacat gcgacgcgac tgaattcgat gccgagcgcc ttttattccg taaacctggt
    31861 ggcgcgcaaa ccggagaagc gtactccaca gaccaacagc tacgcgcgga agtttctgat
    31921 gaactcgcaa tggcgtcccg atcgctgcgc ggtcattgcc ggggcgctgc gttacccacg
    31981 ttatcgctgg tacgaccgtt ttatgctcaa gctgattatg aagatgtcag gcggtgaaac
    32041 ggatacgcgc aaagaagttg tctataccga ttgggagcag gtggcgaatt tcgcccgaga
    32101 aatcgcccat ttaaccgaca aaccgacgct gaaataagca taaagaataa aaaatgcgcg
    32161 gtcagaaaat tattttaaat ttcctcttgt caggccggaa taactcccta taatgcgcca
    32221 ccactgacac ggaacaacgg caaacacgca gccgggtcag cggggttctc ctgagaattc
    32281 cgacagagaa agcaaaaata aatgcttgac tttgtagcgg gaaagcgtat tatgaacacc
    32341 ccgcgc
//
LOCUS       DAZPAB010000023        31735 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZPAB010000023 DAZPAB010000000
VERSION     DAZPAB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.023"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(205..1209)
                     /gene="trpS"
                     /locus_tag="ACHUPG_000999"
     CDS             complement(205..1209)
                     /gene="trpS"
                     /locus_tag="ACHUPG_000999"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR0860709.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(1202..1960)
                     /gene="gph"
                     /locus_tag="ACHUPG_001000"
     CDS             complement(1202..1960)
                     /gene="gph"
                     /locus_tag="ACHUPG_001000"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR0860710.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(1953..2630)
                     /gene="rpe"
                     /locus_tag="ACHUPG_001001"
     CDS             complement(1953..2630)
                     /gene="rpe"
                     /locus_tag="ACHUPG_001001"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR0860711.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(2648..3484)
                     /gene="dam"
                     /locus_tag="ACHUPG_001002"
     CDS             complement(2648..3484)
                     /gene="dam"
                     /locus_tag="ACHUPG_001002"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR0860712.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(3591..4883)
                     /gene="damX"
                     /locus_tag="ACHUPG_001003"
     CDS             complement(3591..4883)
                     /gene="damX"
                     /locus_tag="ACHUPG_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR0860713.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(4975..6063)
                     /gene="aroB"
                     /locus_tag="ACHUPG_001004"
     CDS             complement(4975..6063)
                     /gene="aroB"
                     /locus_tag="ACHUPG_001004"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR0860714.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(6120..6641)
                     /gene="aroK"
                     /locus_tag="ACHUPG_001005"
     CDS             complement(6120..6641)
                     /gene="aroK"
                     /locus_tag="ACHUPG_001005"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR0860715.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(7042..8280)
                     /gene="hofQ"
                     /locus_tag="ACHUPG_001006"
     CDS             complement(7042..8280)
                     /gene="hofQ"
                     /locus_tag="ACHUPG_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR0860716.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVMPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(8192..8598)
                     /gene="hofP"
                     /locus_tag="ACHUPG_001007"
                     /pseudo
     CDS             complement(8192..8598)
                     /gene="hofP"
                     /locus_tag="ACHUPG_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(8588..9028)
                     /gene="hofO"
                     /locus_tag="ACHUPG_001008"
     CDS             complement(8588..9028)
                     /gene="hofO"
                     /locus_tag="ACHUPG_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR0860717.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(9012..9551)
                     /gene="hofN"
                     /locus_tag="ACHUPG_001009"
     CDS             complement(9012..9551)
                     /gene="hofN"
                     /locus_tag="ACHUPG_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFR0860718.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(9551..10330)
                     /gene="hofM"
                     /locus_tag="ACHUPG_001010"
     CDS             complement(9551..10330)
                     /gene="hofM"
                     /locus_tag="ACHUPG_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR0860719.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            10450..13002
                     /gene="mrcA"
                     /locus_tag="ACHUPG_001011"
     CDS             10450..13002
                     /gene="mrcA"
                     /locus_tag="ACHUPG_001011"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR0860720.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(13167..13727)
                     /gene="nudE"
                     /locus_tag="ACHUPG_001012"
     CDS             complement(13167..13727)
                     /gene="nudE"
                     /locus_tag="ACHUPG_001012"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR0860721.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            14047..16182
                     /gene="igaA"
                     /locus_tag="ACHUPG_001013"
     CDS             14047..16182
                     /gene="igaA"
                     /locus_tag="ACHUPG_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR0860722.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            16247..16915
                     /gene="yrfG"
                     /locus_tag="ACHUPG_001014"
     CDS             16247..16915
                     /gene="yrfG"
                     /locus_tag="ACHUPG_001014"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR0860723.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            16926..17327
                     /gene="hslR"
                     /locus_tag="ACHUPG_001015"
     CDS             16926..17327
                     /gene="hslR"
                     /locus_tag="ACHUPG_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR0860724.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            17352..18221
                     /gene="hslO"
                     /locus_tag="ACHUPG_001016"
     CDS             17352..18221
                     /gene="hslO"
                     /locus_tag="ACHUPG_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR0860725.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(18365..20123)
                     /locus_tag="ACHUPG_001017"
                     /pseudo
     CDS             complement(18365..20123)
                     /locus_tag="ACHUPG_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            20502..22124
                     /gene="pckA"
                     /locus_tag="ACHUPG_001018"
     CDS             20502..22124
                     /gene="pckA"
                     /locus_tag="ACHUPG_001018"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR0860726.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(22200..23552)
                     /gene="envZ"
                     /locus_tag="ACHUPG_001019"
     CDS             complement(22200..23552)
                     /gene="envZ"
                     /locus_tag="ACHUPG_001019"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR0860727.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(23549..24268)
                     /gene="ompR"
                     /locus_tag="ACHUPG_001020"
     CDS             complement(23549..24268)
                     /gene="ompR"
                     /locus_tag="ACHUPG_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR0860728.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            24496..24972
                     /gene="greB"
                     /locus_tag="ACHUPG_001021"
     CDS             24496..24972
                     /gene="greB"
                     /locus_tag="ACHUPG_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR0860729.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            25069..27390
                     /locus_tag="ACHUPG_001022"
     CDS             25069..27390
                     /locus_tag="ACHUPG_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR0860730.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            27828..28055
                     /gene="feoA"
                     /locus_tag="ACHUPG_001023"
     CDS             27828..28055
                     /gene="feoA"
                     /locus_tag="ACHUPG_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR0860731.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            28072..30393
                     /gene="feoB"
                     /locus_tag="ACHUPG_001024"
     CDS             28072..30393
                     /gene="feoB"
                     /locus_tag="ACHUPG_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR0860732.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            30393..30629
                     /gene="feoC"
                     /locus_tag="ACHUPG_001025"
     CDS             30393..30629
                     /gene="feoC"
                     /locus_tag="ACHUPG_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR0860733.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            30832..31719
                     /gene="rpnA"
                     /locus_tag="ACHUPG_001026"
     CDS             30832..31719
                     /gene="rpnA"
                     /locus_tag="ACHUPG_001026"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFR0860734.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
BASE COUNT         7631 a         8593 c         8102 g         7409 t
ORIGIN      
        1 atgtggaaac ggcgaggcgc ttcacatttt ttcgggttgt tgcgaaaaga ggataaaaaa
       61 accgggtttc cccggtttca gagtgatgac gaagtgattt attcgacaat gcgagcaaag
      121 ggtaattccc actggcgctt gcctgctccc ctcaccctaa ccctctcccc aaaggggcga
      181 ggggaccgtc cgagcgtttt gatattacgg cttcgccaca aaaccaatcg cttcgtacac
      241 cgctttcagc gtacgggaag cgtgtacact ggctttttct gcgccgtctt tcatcacctg
      301 ttgcaggaag gcttcatcgt tgcggaaacg gtgatagcgt tcctgcaatt cagtcagcat
      361 accggaaacg gcatcagcca cttcaccttt cagatgacca tacatcttgc cttcgaactg
      421 tttttccagt tctgggatgc tctggcccgt taccgcagaa aggatatcca gcaggttgga
      481 aacgcccgct ttgttctgca catcgtagcg aactaccggc ggctcgtcgg agtcagtgac
      541 cgcgcgtttg attttcttca ctaccgattt cggatcttcc agcaggccga taacgttatt
      601 gcggttatcg tcagacttgg acatcttctt ggtcggctcc agcagcgaca ttacgcgcgc
      661 gccagatttc ggaataaacg gctccggcac cttaaagatc tcgccataca gcgcgttgaa
      721 acgctgggcg atatcgcgac tcagttccag gtgctgtttc tggtcttcac ccaccggtac
      781 cagattagtt tgatacagca ggatgtccgc agccatcagc accggatagc caaacagacc
      841 agcgttgatg ttctcggcat aacgcgcaga tttatcttta aactgcgtca tgcgactcag
      901 ttcgccgaag taggtatagc agttcagtgc ccagcctaac tgcgcatgtt ctggtacgtg
      961 ggactgaaca aaaatggtgc ttttctcagg atcgatacca caagccagat acaaggccag
     1021 cgtatccaac gtcgctttac gcagcttctg tgcatcctgg cgcacggtga tcgcgtgttg
     1081 gtcaacgata cagtaaatgc aatggtagtc atcctgcatg tttacccact gacgcagcgc
     1141 acccatgtag ttaccaatgg tcaattcacc tgagggctgt gcgccactaa aaacgatggg
     1201 cttagtcatt tttcgattcc tgattttcgc tatgcggaag ccctaatgcg ggcagaaggt
     1261 catttataga ctgataaatt acatcaggct ggctgagatc gatagcctcg ccgtagttgt
     1321 atccgtaggt taagccaact gatgggcaac ctgccgcttt tgccgcctga atatcattcc
     1381 gtgagtcgcc gacaaacagc atctgttgtg gggcaattcc catccgctca gccaccagta
     1441 acagcgggtc cggatgcggt tttttgtttt gcacatcatc gccgccgata accacgctaa
     1501 agtatttggc aatatctaag gcttcgagca gcggcgcgac gaacggcgtc ggtttgttgg
     1561 tgaccaagcc tagcggcagg cctttagcct gcaacgcgcc caacgtatcg gcaacgtgcg
     1621 ggaacaaaaa cgtcccctct tcggcaacct cgccatagta gcgatcgaac agtttacgca
     1681 gaatacgtac ctgttcttct gccggaatgt cgtcatcaac gggcggtttc cccattgttt
     1741 tacgcagagt cgcacgttcc tgacgcgccc aggtcaatgc gcgctccatc agaacatctg
     1801 cgccgttacc aatccaggta ataacgcgtt cttcacctgc gacgggcaac tccagcgcat
     1861 acagcgccat atctaccgca gcagcaagac caggagcact gtcgaccagc gtaccatcaa
     1921 gatcaaaagc gacgccgcga atatcttcaa acttattcat gacttacctt tgccagttca
     1981 ctgcgcattt catcaatgac ttttttatag tctggctggt cgaagattgc cgaaccggcg
     2041 acgaacatat ccgcgcccgc cgcagcgatt tcgccaatgt tgttcacctt cacgccaccg
     2101 tccacttcca gacgaatgtc aaagccagac tcgtcgatac ggcgacgtac ttcgcgcagt
     2161 ttatccagtg tttgaggaat gaaagactga ccgccgaaac cagggttgac ggacatcagc
     2221 aggatcacat ccagcttatc catcacgtaa tccagatagc tcagaggtgt cgccgggtta
     2281 aataccagac ccgctttaca gccattttct ttaatcagtt gcagcgtgcg gtcaacatgc
     2341 tcggaggctt ctggatgaaa ggtaatgatg ctggcaccag cggcagcgaa atcaggcaca
     2401 atgcgatcga cgggtttcac catcagatgt acgtcgatag gggcggtaat gccgtagtta
     2461 cgcaaggatt tcagcaccat tggcccaatc gtcagattgg gaacatagtg gttatccatg
     2521 acgtcaaaat gcacgacatc agcgccagct gccagggctt ttgcggtatc ttcacccagg
     2581 cgggcaaaat cagccgacag aattgagggg gcaatcaaat actgtttcat ccgcttctcc
     2641 ttgagaatta ttttttcgcg ggtgaaacga ctcctggttt gtacaaagcc agcagttcgt
     2701 ccaccttttt acgtgtgccg ccgttgctgc ttatactgcg tcgaactttg acgacatgca
     2761 attttgcgcg ctgataccac tcacgcgtta acatcgtatc gtgattggag atcagcactg
     2821 gaatatggcg ctcaaccaga ccttcggcga tctccgccag atgcgcttgt tgttcaagcg
     2881 taaaactgtt tgtgtgatac gccgtaaagt tggcggtcgc agacagcggt gcataaggcg
     2941 gatcgcaata gacgacggat gcatcatctg cgcgctccat gctatcggcg taagactcac
     3001 aatagaaaaa ggcattctgc gctttttcag cgaagtgata caactctgct tccgggaaat
     3061 agggtttttt gtagcggcca aacggcacgt taaactcacc gcgcagattg taacgacaca
     3121 ggccgttgta accgtagcgg ttcaaatata aaaacagtac cgcccgacgg aacggatcct
     3181 ggcttttgtt gaactcttcg cggaactgat agtaaacctc ggcgcaattt gtttcgggaa
     3241 caaacagctc gcgtgcggcc tgtacgtact catcagtacg catcttcaca atgttataga
     3301 gactgatcag gtcgctattg atatcggcaa ggatataacg agaaaagtcg gtgttgagaa
     3361 acaccgaccc ggcacctaca aaaggctcaa ccagacattc gcccttgggc aaatgccgtt
     3421 taatatcatc aagcagggga tacttgcccc ctgcccactt caaaaaagcg cgatttttct
     3481 tcatgccgat taactaatta caccttctcc ggctgtggag aaagctccga cagcatccag
     3541 cgcttcaggc agctcccgca acgataaacc attgcgggag ataaccttga ttacttcaga
     3601 tcggcctgta cctgacgcag cggtttcgcc cacgggtttt tggcctgaac atctgctggc
     3661 aatgtagata ccgctttttt cgcctcttct ttcgaagcgt acacgccaga aaccaggaca
     3721 taccacggct gaccattacg cgtcgtttca tagacaacgt agtttttcag attctctttc
     3781 ttcgcccaac cgttcaggtt gtcgtagtta gaggaactgc tcagctgcag agtgtaatgg
     3841 ctggacggtg ccgatttcaa cgaaccaaca ttacctgcgg tcttccctcc agcggctggt
     3901 gttgccgtgg tttgcgccgg ggatgccgtc tgtactggag ccgtagtcgc cgtctctttt
     3961 ggtgctggcg tagaagtcgc agccggtgcc ttcgtgctcg ccacaggggc agctggttca
     4021 ggttcagtac gcttcggcgt ctgtgctacc ggcttcggct ccgttttcac ggttgcttgc
     4081 ggttttttcg gttcaatcac cgcctgctga cgaactgggc gcgtagtgga cggacgttca
     4141 gcggtttgcg ttttcgccgt gtcacgcgat gcattgccat tgcgaacagg cgcaaccgtt
     4201 gcgggttcag tcggcaacgt ggaattgacc gccacattgt tcagctgttg ctgattttgt
     4261 ggctgggtca gcgcattgtt caggtcaccc tgcacttcaa cacgttgttg accatccgtt
     4321 gccaccgggg tttgcccttg agtcggcgta gaagagatcg gtggcagaga aacatcctgc
     4381 tgagtatttt ccgcagacgt ggttcccggc gctggctgca cgccattcgc ctgatcggtc
     4441 gcattgccag caagatcaat actcttctcg ccagacgcgg tttgatcgct ggaagtggtc
     4501 gagggggctt ttagcgcaga accgataccg atgatcaaca gcagtagaac cagaatgccg
     4561 acgcccatca tcatatactg acgagaagcg ggtttactgg ctgctttttt gcgcttacgc
     4621 ggacgacgct ctacgcgctc ttcatccacg gtttcatctt cggattcgtc aatttcttct
     4681 tcgatttccg gttcctcatt gcgctctttt tgcgcacgag tcggacggcg atcgtcagtg
     4741 tcatcaagtt caatatcatc aaaattgatc tgcggttcgc cacgttcagt acgctcagaa
     4801 cgttcagaag attgacgaga acgaccagta cgacgatcgc tgggatcggg tttcagctcg
     4861 tcttctggtt tgaattcatc catttaacac cccactaaaa ggttaatgct taccacgttg
     4921 caattaacct gaagctaata gaccgcttga taagcggcct gacctttctt gttgttacgc
     4981 tgattgacaa tcggcaatgg cgttaagaac aagctcgtgc gaaacgccgc tgcgaacttc
     5041 actcttacca attgccaacg gaagaattaa gcgcatctct cccgcaagga ctttcttgtc
     5101 acgcagcata tgcggtaaat acgcctgcgc ggacatttcg cgcggcccat tgaccggtaa
     5161 cccagcccgc gtgagcaggg ttataatacg ctgcgtttcg gcagaactaa actgcccgag
     5221 acgttccgac gtccgcgccg ccatcaccat acccgcagcg accgcttcac catgtaacca
     5281 attgccatac cccatttcag cttcaatggc atgaccaaag gtgtgtccca gattcagtaa
     5341 agcacgtaac ccggtttcgc gctcgtcggc ggcgacaact tctgccttca gttcacaaca
     5401 acggcgaata cagtacgcca ttgccggacc gtccagacgc aacaacgcat ccagattctc
     5461 ttccagccag ttaaaaaacg caccgtcaag aataatgccg tatttgatga cttctgccag
     5521 ccccgacgct aactcacgcg ggggaagcgt tttcagacag tcgagatcca ccaccactga
     5581 agcaggctgg tagaacgcgc caatcatgtt tttaccgagg ggatggttga ccgcagtttt
     5641 gccgccaacg gaggaatcga cctgcgacag taacgtcgtc gggacttgaa tgaaacgaac
     5701 accgcgctga taactcgccg ccgcgaagcc ggtcagatcg cccactacgc cgccgccaag
     5761 cgccaccagc gtagtatcgc gaccatgcgg cttttgtaac aacgccgtaa agacggtatc
     5821 gagtacagct aggcttttat actgctcgcc gtcagggagg ataacgctat cgacgttaac
     5881 acccgcctgt tcaagtacgc cgcggacctt atcgagatac agaggagcca gggtttcgct
     5941 ggtgaccaac atgacctgct cgcccgattt cagcggtaag aatgaagctg gttcattaaa
     6001 caaaccagat gcgatggtaa ttgggtaact acgttccccg agagtaacga caatcctctc
     6061 cataacgcga catccacctt aattactgta cccgcagacg agtgtatata aagccagaat
     6121 tagttgcttt ccagcatgtg aataatctgg tttgcaacca ctttagcgct ttgatcatca
     6181 gtacgaatgg tcacgtcggc aatctcttca tacagcggat tgcgttcatt ggccaacgct
     6241 tccagaactt cacgcggcgg tgtttcaacg tgcagcaacg ggcgtttttt atcacgctgc
     6301 gtgcgtgcaa gttgcttttc gatggtcgtt tcaagataaa cgacaacgcc acgagcggaa
     6361 agacggttac gcgtttcacg ggatttcaca gagccgccgc cagtagccag cacaataccc
     6421 tgtttctcgg tcaactcatt gatgaccttt tcttcgcgat cgcggaagcc ttcttcgcct
     6481 tctaaatcga aaacccagcc cacatcagct ccggttcgtt tctcaatctc ttgatcggaa
     6541 tcgtaaaatt ccatattgag ttgttgagct aactggcgcc caatagtgct ttttccggca
     6601 cccataggcc caaccagaaa gatattgcgt ttctctgcca ttttttcggt actactaaga
     6661 ctattcgtta atgataaacc cgcttcgctc agagagcgcc gcaggacatg aactgaaacc
     6721 tcataagata ttgcgagagt cagactgaaa attatctcaa tactccagcg ggtttggcaa
     6781 ctgaataaat cacccatccc tctgcatatc tggtcgctgc aagcgcgctg ccttgctacc
     6841 accgctctgg cgataaatca ccgggtaaga ttagcgtaaa aaagacagca aaatgccgcc
     6901 tgaatgataa atcatcatca tggaacgcag gcagcgaaga gcaccaactc tcaaatcggt
     6961 actccttgta tgctaaatac ctgcgcgcgt caaatagatg aaacacgttc agcataaaaa
     7021 cattaccgct tttacggctg tttactcact ggaaaccagt cgtggcgtga taaacaccac
     7081 taactcgcgt cgttcatctt cttttccgtc atgacgaaat aattgcccga accagggaat
     7141 gtcgccaagc aacggtacgc tatcctgacc cgatttattt ttacgggtaa aaatgccgcc
     7201 cagcgccaac gtttctccgc tttttacctc gacctgcgtt tcgatctcct gcttatcaat
     7261 cgccagcact tcgccatcgg cctgctgtag cacctgcccc ggaacgttct ggctgatgtg
     7321 taatttcagc cggatgcgac ctttttgtaa caccgtgggc atgacctcca tccccaggac
     7381 ggcctcttta aattccaccg acgtcgcgcc actttccccg ctggaaacct gatatggaat
     7441 ttcgctctcc tgtttaatgc tggcaggctg aagatgtgag gccagcagac gcggactggc
     7501 gataatatcc agctgctgtt tttgttcgag cgcggaaagc tcaagatcca gcaagcgtcc
     7561 gttgatgcgc ccaatgttaa aaccgacatg cgttgtcgcc gtcgctacgg agaggtcgct
     7621 accaagcgtg gtgacttgcc caacgccacc agcgtgttgc gcatcggcca gcgtccattt
     7681 cacgcctaac tcacgcaaac ttttttcatt aatggtgaca atatgcgccg acagctcaac
     7741 ctgcccgacc ggcagatcca tttgcgctac ccactgttca agcgcgctta acgccgtttt
     7801 gttatctcgt agcaaaaggc gattggtgcg tttatcgacg gtcatactcc ctttggcact
     7861 cagtagcttc tcccccgctt tcgccagttc tcccgcgtcg gcgtattgca gggttatact
     7921 gcgattttcc agcggcagat ttgcctgcgc ccgcgcctgc tccgcctcct ggcgggcgat
     7981 attgtcattc tgccaggcaa tggaatgcac tgagagaatg ttgccttcct gccgcgttat
     8041 cagtccggcg cttttcacta cagtttgtag tgcctgcttc cagggaacat ctgttagatg
     8101 taacgacacc gtaccgctga cgtctggcga cacgaccagg ttcaacttct cctgttcagc
     8161 cagcgcctgc aacacctgag ctaccggaac gtcatccacc atcagcgtca ctttttgcgg
     8221 ctttgccgcc tgtacgccgg gtatcagcat caacagtagt gcggctatcc attgcttcat
     8281 ttgtatctcc ttgccgttgc cacaaccatt gtggcggttc gcagtttgtc ccggtaccca
     8341 gcgttagtac gtctggcgtc agctgtaaaa ttgtccagcc gttttccagc acatcgtttt
     8401 gctgcacccg tcgccatttc ttttgcccgt cttttattac accgatgatg cgctcgcctc
     8461 gccctaccat cccctgatag cgccactggc taagttcgct aatccggcat atagatcttc
     8521 cggcgattta aaagggtcac gcataccggt taaaaggcac aatgcaatac ctgccaacaa
     8581 ccagcgttta accctcatgc ggcatctcca gttgtagcgt gaacaaaaga tcatcacctt
     8641 ccacgcttaa cgaaaaacgg ctcacgctga cgttgcgctc tgccagccgt gtaaatgccg
     8701 acggcactgc ttcccacagc gttttcaacg ccaactcacc tccctgcgcg gatggatgcc
     8761 aggaaaccag ttgcgcgccg gataactgaa aatccagtgg cgaaaagggc agcgtttttt
     8821 cctcgctgaa gggagtggtg tctaccaggc gatacagtgc ggcccactgc tggtgatgac
     8881 ttgcccgtag tcgaattaat gcgtcgcgct cttcatggtg tgtcgatgat agaaaaatga
     8941 gcgtaactaa catcagcaac cagaatgccc agcaaaactg gcggaggcgg ggtgatgtgg
     9001 cgaaccacca gtcaaagaac atgttcatcg ctaaccttcc ttgttaactg atactcaaat
     9061 tgccagcgtc cctgcgcatc ctgctgcgtg gctccccgct gattgagatg aaaagacgca
     9121 tcctggcgga gtgacgtttc tagtgcgttt aacgcggtaa tgcttgttgt cagccccttg
     9181 atctccagcg ttccctgctg ccagcttatc gttgtcagcc aggcgtgctc gggtaaaagc
     9241 gccgccagtg cttccagcgc agattgccag tcgcgggtaa attgtcgctg gcgctgccgt
     9301 tgcgaacgct gttcgcgtaa ctgctgttgc tccagcaaac gtggcttcgt tatctgtaag
     9361 ctgcgggcga gttgttgttc cgcctgaagc aaaactgcgt ctatgcgcgc ttcggcgctg
     9421 cctgtcagac gcagtattag cgttatcccg acggccagca gcagaggcgc aacgaacatc
     9481 agcaaccaga aacgcagaaa agcggtccgg cgttgctgtc gccagggcaa aaaattaatt
     9541 ggcggattca tcagtacgcc ttcccaagcg ccagccccag cgcgatggca aagtctccac
     9601 caggcggcgg tagcggcggc tgacgaacag aaacggcctc ccaggggtca aatccgcctt
     9661 cgccacatat cgcgacgctg tcgggatcaa cggataacgc tgccgccagc tccttcgcgc
     9721 tagtcatccc caccgccagt ttgcgccccc agctatagcg cgtcgcccac agccactgtt
     9781 cgttatcacg ccaggccaga cattgctgat gagaaggtaa aaaaggcagg aatcgctgta
     9841 atgcactggc atccggggtg atcgcactaa catgaacacg caacctttct gccagcgtaa
     9901 gcagcgttgc cagctctttg ctttgcgcgg cagtcacgtt ataagcgggg ctgagtgagt
     9961 cttcgctgta atcgaagcgc agggagtccg gatccatatc cagctcgcgg gccatcgtcc
    10021 ctgacagcca ggccgtttgc tcccgctcac caagggacat cgacgggcgc ggaaatgacc
    10081 gctgtaatgt gcgactggcg ggaaacgcca acataatgtg atgacgctgc ggcagttcgc
    10141 gactccacgg taacaacgtt ttagccagct gctgcgcatc aacaatccgc ccatctttga
    10201 taatgtcgtg cgccagcggc aaccgccacc agcgttgcaa aaagcattct tttgcgcccc
    10261 gcacgatcgc aaccgctgcc gcttcttgct gttgtaaatg caaaccaatt tgccagatct
    10321 taaatgtcat tgtgatgatc tccttatcac ccgtcactct gacgggtata tcaatgcgtc
    10381 tggcttgcct ttatactacc gcgcgtttgt ttataaactg cccaaatgaa actaaatggg
    10441 aaatttccag tgaagttcgt aaagtatttt ttgatccttg cagtctgttg cattctgctg
    10501 ggagcaggct cgatttatgg cctataccgc tacatcgagc cacaactgcc ggatgtggtg
    10561 acattaaaag atgttcgcct gcaaattccg atgcagattt acagcgccga tggcgagctg
    10621 attgctcaat acggtgagaa acgtcgtatt ccggttacgt tggatcaaat cccaccggag
    10681 atggtgaaag cctttatcgc gacggaagac agccgcttct acgagcatca cggcgttgac
    10741 ccggtgggga tcttccgtgc ggcaagtgtg gcgctgttct ccggccacgc gtcacaaggg
    10801 gcgagtacca ttacccagca gctggcgaga aatttcttcc tcagtccaga acgcacgatg
    10861 atgcgtaaga ttaaggaagt cttcctcgcg attcgcattg aacagttgtt gacgaaagac
    10921 gaaatcctcg agctttatct gaacaagatt taccttggtt accgcgccta tggtgtcggt
    10981 gctgcggcac aagtctattt cggtaaaacg gtcgaccaac tgacgctgaa cgaaatggcg
    11041 gtgatagccg ggttgccgaa agcgccttcc acctttaatc cgctctactc aatggatcgt
    11101 gccgtcgcgc ggcgtaacgt cgtactgtcg cggatgctgg atgaagggta tatcacccag
    11161 caacagttcg atcagacacg cactgaggcg attaacgcta attatcacgc gccggagatt
    11221 gctttctctg cgccgtacct gagcgaaatg gtgcgccagg agatgtataa ccgttatggc
    11281 gaaagtgcct atgaagacgg ttatcgcatt tacaccacca tcacccgcaa agtgcagcag
    11341 gccgcgcagc aggcggtacg taataacgtg ctggactacg acatgcgcca cggctatcgc
    11401 ggcccggcaa atgtgctgtg gaaagtgggc gagtcggcgt gggataacaa caagattacc
    11461 gatacgctga aggcgctgcc gacctatggt ccgctgctgc ctgccgcagt caccagcgcc
    11521 aatcctcagg gagcgacggc gatgctggcg gacgggtcga ccgtcgcatt gagtatggaa
    11581 ggcgttcgct gggcgcgtcc ttaccgttcg gatactcagc agggaccgac gccgcgtaaa
    11641 gtgaccgatg ttctgcaaac gggtcagcaa atctgggttc gtcaggttgg cgatgcatgg
    11701 tggctggcac aagtgccgga agtgaactcg gcgctggtgt cgatcaatcc gcaaaatggt
    11761 gccgttatgg cgctggtcgg tggctttgat ttcaatcaga gcaagtttaa ccgcgccacc
    11821 caggcactgc gtcaggtggg ttccaacatc aaaccgttcc tctacaccgc ggcgatggat
    11881 aaaggtctga cgctggcaag tatgttgaac gatgtgccaa tttctcgctg ggatgcaggt
    11941 gccggttctg actggcagcc gaagaactca ccaccgcagt atgctggtcc aattcgctta
    12001 cgtcaggggc tgggtcagtc gaaaaacgtg gtgatggtac gtgcaatgcg ggcgatgggc
    12061 gtcgactacg ctgcagaata tctgcaacgc ttcggcttcc cggcgcaaaa cattgtccac
    12121 accgaatcgc tggcgctggg ttcagcgtcc ttcaccccaa tgcaggtggc gcgcggctac
    12181 gcggtcatgg cgaacggcgg cttcctagtg gacccgtggt ttatcagcaa aattgaaaac
    12241 gatcagggcg gcgtgatttt cgaagcgaaa ccgaaagtag cctgcccgga atgcgatatt
    12301 ccagtgattt acggtgatac gcagaaatcg aacgtgctgg aaaataacga tgttgaagat
    12361 gtcgctatct cccgcgagca gcagaatgtt tctgtaccaa tgccgcagct ggagcaggca
    12421 aatcaggcgt tagtggcgaa gactggcgcg caggagtacg caccgcacgt catcaacact
    12481 ccgctggcat tcctgattaa gagtgctttg aacaccaata tctttggtga accaggctgg
    12541 cagggtaccg gctggcgtgc aggtcgtgat ttgcagcgtc gcgatatcgg cgggaaaacc
    12601 ggtaccacta acagttcgaa agatgcgtgg ttctcgggtt acggtccggg cgttgtgacc
    12661 tcggtctgga ttggctttga tgatcaccgt cgtaatctcg gtcatacaac ggcttccgga
    12721 gcgattaaag atcagatctc aggttacgaa ggcggtgcca agagtgccca gcctgcatgg
    12781 gacgcttata tgaaagccgt tcttgaaggt gtgccggagc agccgctgac gccgcctccg
    12841 ggtattgtga cggtgaatat cgatcgcagc accgggcagt tagctaatgg tggcaacagc
    12901 cgcgaagagt atttcatcga aggtacgcag ccgacacaac aggcggtgca cgaggtggga
    12961 acgaccatta tcgataatgg tgaggcacag gaattgttct gattaaaaag gcgcttcggc
    13021 gccttttcag ttggctgaca aagtgcattt gtttatgccg gatgcgggtg aacgcgttat
    13081 ccggctaaca aaatcgtgaa aaatcaatat attgcaggaa ccccataagc ctgataagcg
    13141 ttgtgcatca ggcaaacttc acgcatttac actcgcccct gccctttcaa ccattcgcgc
    13201 acgaggaaca gcgcgctgac attacgcgct tcgttgaagt cagggtcttc cagcaaatcc
    13261 atcatatgcg ccagcggcca gcgcacctgt ggtagcggct ctggctcatc gccttccagc
    13321 gattccgggt agagatcttg cgcgaccacg atattcattt tgctggaaaa gtaagacggt
    13381 gccatgctga gcttcttcaa aaaagtcaga tcgttcgcac caaatccaac ctcttctttt
    13441 agctcgcggt tagcggcttc gtagacgctt tcacccggat caattaatcc tttcgaaaaa
    13501 cctaattcgt aggattcagt tcccactgcg tattcgcgga tcaggatcag gtgatcgtcc
    13561 acaatcggca caatcatcac tgcttcccgg ttggttggac gcattcgttc ataaacacgc
    13621 cgcacgccat tgctgaactc cagatccacg ctctcgacgg taaacagtcg ggaacgggct
    13681 acagtttcaa cattcagaat ggtgggtttt tgtaatgatt tgttcatcgt gggatctatg
    13741 ctgtgaaatc agcggttatt gtgcgatatc ggacacgctt tcggcaatgt gaattgcatg
    13801 ttatttacat ttatgtaact taataaataa tcgtcctcaa atcaaattaa aagtcaatag
    13861 gttgaaataa ctccaggaat ttgctgatat tccgccttca gagggtttgc tatgatcagc
    13921 ggttactgtg atgtgcttaa tgatgctcaa gttaagctcc acgcttgccg atagccaacc
    13981 gcagaatcat gtattgtgtc cggtgcgact gaccacgcct gacagactaa gtaagatggg
    14041 gaaagcatga gcaccattgt gattttttta gctgctttgc tggcctgctc actacttgcg
    14101 ggatggctga taaaagtgcg atccagacgg cgtcagctgc cctggaccaa cgccttcgcg
    14161 gatgcgcaaa cgcgtaaact cacacctgaa gaacgtagcg ccgttgaaaa ttatcttgag
    14221 agcctgacgc aggtattaca ggtgcctggc ccaacggaag ctagcgcggc accgatctct
    14281 ctggcgctga atgccgaaag caacaacgtc atgatgctga cacacgctat cacacgttac
    14341 ggcatctcta ccgacgatcc gaataaatgg cgttactacc tcgattcggt agaagtccac
    14401 ctgcccccct tctgggaaca gtacatcaac gatgagaata ccgttgaact gattcatacc
    14461 gattcgctgc cgttggttat ttcactcaac ggtcatacgc tgcaggagta catgcaggaa
    14521 acgcgcggct atgccttgca acctgttccg tccacgcagg cgtcgattcg cggggaagag
    14581 agtgagcaaa tcgagctact caatattcgc aaagaaacgc atgaagaata tgcgctgagt
    14641 cgtccgcgcg ggctgcgtga agcgttgctg atcgtcgcct ccttcctgat gttctttttc
    14701 tgcctgatta ccccggatgt atttgttccg tggctggcag gcggcgcatt actgctgctg
    14761 ggcgcaggtc tgtgggggct attcgcgccc ccggcaaaat cctccctgcg agaaattcat
    14821 tgtctgcgcg gtacaccccg tcgttgggga ttgtttggcg aaaacgatca ggaacagatc
    14881 aacaatattt cactcggtat tatcgacctg gtctatcccg cacactggca gccatacatt
    14941 gctcaggatc tcggtcaaca aaccgatatt gatatctatc tcgaccgcca cgtagtgcgt
    15001 caggggcgat atctttcgct gcatgatgaa gtaaaaaact ttccgttaca gcactggctg
    15061 cgcagtacga ttatcgctgc gggctcgctg ctagtgctgt ttatgctgtt attctggatc
    15121 ccgctggata tgccgctgaa attcactctc tcatggatga aaggcgcgca gaccattgaa
    15181 gccaccagcg taaaacaact ggctgacgct ggcgtgcggg taggcgatac attgcgtatt
    15241 agcggtacgg gaatgtgtaa tattcgcact tccggaacct ggagcgcgaa aaccaattca
    15301 ccttttttac cgtttgactg ctcgcagatc atctggaatg acgcccgctc attaccatta
    15361 ccagaatctg aactggtcaa caaagcgacg gcattgactg aagcagttaa tcgccagctg
    15421 caccctaaac cggaagatga atctcgcgtc agtgcctcat tacgttcagc gattcaaaaa
    15481 tccggcatgg tattgcttga tgattttggc gacattgtac tgaagacagc ggatttatgt
    15541 tctgccaaag atgactgtgt gcgactgaaa aatgcgctgg tcaatctcgg caacagtaaa
    15601 gactgggacg cgctggtaaa acgcgccaac gccgggaagc tcgatggcgt gaatgtgtta
    15661 ttacgcccgg tgagtgctga atcgctggat aacctggtgg caacctccac cgcgccgttt
    15721 atcacgcatg aaacggcgcg agcggcacaa tcactaaaca gtccggcccc cggcggattc
    15781 ctgattgtca gcgacgaagg cagcgatttt gttgatcagc cctggccttc ggcatcactt
    15841 tacgactacc cgccgcaaga acagtggaac gctttccaga aactggcaca aatgctgatg
    15901 catacgccgt ttaacgccga aggtatcgtc acaaaaatct tcactgacgc caatggtacg
    15961 cagcatattg gccttcatcc gatcccggat cgttccggcc tgtggcgcta tctcagcacc
    16021 acattgctgc tactgacgat gctgggtagc gccatttaca atggcgtaca ggcctggcgt
    16081 cgttaccagc gtcatcgcac tcgcatgatg gagattcagg cctattatga aagctgcctg
    16141 aacccgcaac tgatcacccc ttcagaaagc cttatcgaat aacacgtttg cgcggcaggt
    16201 tatgctaccc tgtcgcgcaa attgcttcac tctggagatt tccctcatgc atatcaacat
    16261 tgcctggcag gacgtagata ccgttctgct ggatatggac ggcacgttgc tcgacctcgc
    16321 cttcgataac tatttctggc aaaagctggt gcctgaaaca tggggcgcga aaaacggggt
    16381 tacgccacag gaagcgatgg aatatatgcg ccagcaatat cacgacgtac agcatacgct
    16441 aaactggtac tgtcttgatt actggagtga gcaactgggt ctggatatct gtgcgatgac
    16501 caccgagatg ggaccgcgcg ccgtactgcg tgaagatacc attccgtttc ttgaggcact
    16561 gaaagccagc ggtaagcagc gaattttgct caccaatgct catccgcaca acctggcggt
    16621 aaaacttgag catacgggtc tggacgcaca ccttgattta ttactttcca cccacacatt
    16681 tggttatccg aaagaggatc agcggttatg gcatgcggtg gccgaagcta cgggtctgaa
    16741 agctgaaaga acgctgttta ttgatgacag cgaagcgatt ctcgatgctg ccgcgcaatt
    16801 tggtattcgt tactgcctcg gcgtgactaa tcctgattcc gggattgccg agaaacagta
    16861 tcaacgccat ccgtcactga atgactaccg ccgcctgatc ccctcgctaa tgtgaaggag
    16921 acgccatgaa agagaaacct gctgttgagg ttcgactgga taaatggcta tgggctgccc
    16981 gtttttataa aacccgcgcg ctggcccgtg aaatgattga aggcggtaag gtgcattaca
    17041 acgggcagcg cagcaagccg agcaaaatcg tcgagctgaa tgccacgctc actctgcgcc
    17101 agggaaatga cgaacgcacg gtgattgtaa aggcgattac tgaacagcgt cgccccgcca
    17161 gcgaggcagc cttgctgtat gaagagactg cggaaagtgt agagaaacgc gaaaaaatgg
    17221 cgatggcacg taaacttaat gccttaacca tgccgcaccc ggaccgacgc ccggacaaaa
    17281 aagagcgccg cgacctgtta cgatttaaac acggcgacag tgaataactg tcacctgcaa
    17341 gagagatgat tatgccgcaa catgaccaat tacatcgcta tctgtttgaa aattttgccg
    17401 ttcgcggcga actggtaacc gtttcggaaa ccctgcaaca gatccttgag aaccacgatt
    17461 atccgcagcc cgttaaaaac gtgctggcag aactgctggt tgcgaccagc ctgttaaccg
    17521 ctacgctgaa gtttgatggt gatatcaccg tacagctgca gggcgacggt ccgatgaatc
    17581 tggcggttat taacggtaac aataaccagc aaatgcgcgg tgtggcgcgc gtgcagggcg
    17641 aaattccaga aaatgccgac ctgaaaacgc tggtcggcaa tggttacgtg gtgatcacca
    17701 ttaccccgag cgaaggcgaa cgctatcagg gcgtagttgg tctggaaggt gataccctgg
    17761 cggcctgcct agaagattac tttatgcgtt ctgaacagct gccgacgcgc ctgtttattc
    17821 gcaccggcga cgtagacggc aaaccggctg caggcggtat gttgttgcag gtaatgcctg
    17881 cgcaaaatac ccaacaggac gactttgacc acctggcgac gctaaccgaa accatcaaaa
    17941 ccgaagaact gctgacctta ccggcaaacg aagtgttgtg gcgtttgtat cacgaagaag
    18001 aggtgacggt ttacgatccg caggatgtgg agttcaaatg cacctgctcg cgtgaacgtt
    18061 gcgccgatgc gctgaaaacg ctgcctgatg aagaagttga tagcatcctg gcggaagatg
    18121 gcgaaattga catgcattgt gattactgcg gtaaccacta tctgttcaat gcgatggata
    18181 ttgctgaaat ccgcaacaac gcgtctccgg cagatccgta agttcattaa tgagtatgtc
    18241 cggcagagag ggctctgccg gattttacga cttaatggcc tgatgtgctg cgcttaccag
    18301 cgcctatgaa ataaatcgca aattattttg gtcagataca gcgtcgtatc cgataaaagc
    18361 gttgtcactt acttaagttt atttccagcg tttcaccatc taccgtcaga tcgcgccacg
    18421 ctttcggcct cgtccccagt tttccatcct tcgcagctgt gagtaatttt tctttcgtta
    18481 tttcgttcgg aagtaaactc atatcaagcg cgtcccattc ttttctgtct gagtcaaagc
    18541 catagacaat gactctgtca tcattaaaag caaacaggat ccgctccgcc tgaccatcac
    18601 tattcagatc ttgctcgacc agtacgcagg cgtctttttc aatacaggtc atcacgttat
    18661 agcgatcctg gattaaggcc gaccagaatg tcgcatccgg tttaacagaa ccaggggcaa
    18721 ttaacacatt ttcggcgaat actttttccg atacctgttc ctgaagatgt tgctctccat
    18781 ctaacgccat cagcagatcc cgcgcgcgtt tcgggtcttt cataaacccg gcatcgcttt
    18841 tcagcgactc aagcgccgca cgtccatagc gaccgctctg ttcgagcatg tagatagtta
    18901 cctggtctgg cgtgtttttg ccgctctgat aacgcgccat atgactgttc acgctaatac
    18961 gcatactgtc cagcaccggc gaattaagaa gcaccagtat caccaacacc aataacgaaa
    19021 ccgcaaggtt cactttgccc tgaagaacaa ggggattttg cccattacgc cagacgatac
    19081 tgacaaaata ccccagcgac cacaccagta acaccagctc cgccagcgcg ccctgcaagc
    19141 ggtcgacagt ccagccgtac tgagcgaccc gcagccataa cccccaggtg gcgacgaaca
    19201 catacagcgg agcaaccagc aaagcggttt taatcaggca acgcaacggc cccgtccagg
    19261 gaagtgacgc tttttgcgga tcgcggacaa tagccattaa gatcaattgc aaaaaggcca
    19321 gcgttaacag caacccggcg gcggagatgt ggcgagaaat cgcgctcagg cccgtaaacg
    19381 gcagggtgat gataaacatc agggttagca atgatactaa cggcagcaac cccgtggcga
    19441 ttagcgtgaa caacttttga atagagtcga ttaaacgtga ctgtgtacgc gcgaggatca
    19501 ccgccagtgc ggtaaccaga ccaaatgtaa gatacataaa ccagtcggtt gcaaaaaaga
    19561 gcgtattgaa aaacgtgatg ccaacaagtt taaacaactc accccacagt aaaagcacca
    19621 gccacgtcag gccattggag agaaaaatca ccagtaatat aagtacgtta tgccatactg
    19681 actggtaaaa gtagctatag cggctactgc cattgcggat acgaaggctt tgttgtatcc
    19741 acggtagcaa caacattgcc atcaacagca gatagcagcc aaaatcccaa atcgcctttt
    19801 cagctctcca ggggttcatg ccatcagttt gccacttcag ccaaccgctc atccctgtcg
    19861 tggcaataaa caccagcgcc agccatccct gtcgtggcaa taaacaccag cgccagccat
    19921 ccccaaaggc gcttctgttt aaaagagatc acggagaaga gtaaaacgga tgaaaacgcg
    19981 acggtcgcag gcacgccata aacaatccaa ctgtgatttt ttccggcaag ccaggcgata
    20041 agcaggtagc aaaccagccc ctgaagtaat ccggtagcaa tcatccccca gcgtgttgcc
    20101 ggtgaaagtt caacgttatc catgttggtt atccagaatc aaaaggtggg ttaattatcg
    20161 catccgggca gtagtatttt gcttttttca gaaaataatc aaaaaaagtt agcgtggtga
    20221 atcgatactt taccggttga atttgcatca atttcattca ggaatgcgat tccactcaca
    20281 atattcccgc catataaacc aagatttaac cttttgagaa cattttccac acctaaaatg
    20341 ctatttctgc gataatagca accgtttcgt gacaggaatc acggagtttt ttgtcaaata
    20401 tgaatttctc cagatacgta aatctatgag ccttgtcgcg gttaacaccc ccaaaaagac
    20461 tttactattc aggcaataca tattggctaa ggagcagtga aatgcgcgtt aacaatggtt
    20521 tgcccccgca agaactcgag gcttatggta tcagtgacgt acatgatatc gtttacaacc
    20581 caagctacga cctgctgtat caggaagagc tcgatccgag cctgacaggt tatgagcgcg
    20641 gggtgttaac taatctgggt gccgttgccg tcgataccgg gatcttcacc ggtcgttcac
    20701 caaaagataa gtatatcgtc cgtgacgata ccactcgcga tactttctgg tgggcagaca
    20761 aaggcaaagg taagaacgac aacaaacctc tctctccgga aacctggcag catctgaaag
    20821 gcctggtgac caggcagctt tccggcaaac gtctgttcgt tgtcgacgct ttctgtggtg
    20881 cgaacccgga tactcgtctt tccgtccgtt tcatcaccga agtggcctgg caggcgcatt
    20941 ttgtcaaaaa catgtttatt cgcccgagcg atgaagagct ggcaggtttc aaaccagact
    21001 ttatcgttat gaacggcgcg aagtgcacta acccgcagtg gaaagaacag ggtctgaact
    21061 ccgaaaactt cgtggcgttt aacctgaccg agcgcatgca gctgattggc ggcacctggt
    21121 acggcggcga aatgaagaaa gggatgttct cgatgatgaa ctacctgctg ccgctgaaag
    21181 gtatcgcttc tatgcactgc tccgccaacg ttggcgagaa aggcgatgtt gcggtgttct
    21241 tcggcctttc cggcaccggt aaaaccaccc tttccaccga cccgaaacgc cgcctgattg
    21301 gcgatgacga acacggctgg gacgatgacg gcgtgtttaa tttcgaaggc ggctgctacg
    21361 caaaaactat caagctgtcg aaagaagcgg aacctgaaat ctacaacgct atccgtcgtg
    21421 atgcgctgct ggaaaacgtc accgtgcgtg aagatggcac tatcgacttt gatgatggtt
    21481 caaaaaccga gaacacccgc gtttcttatc caatctatca catcgataac atcgttaagc
    21541 cggtttccaa agcgggccac gcgactaagg ttatcttcct gactgctgat gctttcggcg
    21601 tgttgccgcc ggtttctcgc ctgactgccg atcaaaccca gtatcacttc ctctctggct
    21661 tcaccgccaa actggccggt actgagcgtg gcatcaccga accgacgcca accttctccg
    21721 cttgcttcgg cgcggcgttc ctgtcgctgc acccgactca gtacgcagaa gtgctggtga
    21781 aacgtatgca ggcggcgggc gcgcaggctt atctggttaa cactggctgg aacggcactg
    21841 gcaaacgtat ctcgattaaa gatacccgcg ccattatcga cgccatcctc aacggttcgc
    21901 tggataatgc agaaaccttc actctgccga tgtttaacct ggcgatccca accgaactgc
    21961 cgggcgtaga cacgaagatt ctcgatccgc gtaacaccta cgcttctccg gaacagtggc
    22021 aggaaaaagc cgaaaccctg gcgaaactgt ttatcgacaa cttcgataaa tacaccgaca
    22081 cccctgcggg tgccgcgctg gtagcggctg gtccgaaact gtaatgattt gaagctggag
    22141 aatatctatc cagtatctta tagaaagcaa aacgggaggc accttcgcct cccgtttatt
    22201 tacccttctt ttgtcgtgcc ctgcgcccgc gttaccggca ctggcagcca ggcgcgaatg
    22261 gaaagcccgc cccgctcgct ggtgccaagc tccagcatcc cgttatggtt atccacgata
    22321 cgctgcacaa ttgccagccc taatcccgtg ccgctaatgg tgcgcgcact gtcgccgcgg
    22381 acaaacggct ggaacaggtg cttacgttgt tccggcgcaa ttcccggacc gtcatcttcc
    22441 acctggaacc aggcgcgatt cggctccgtt ccgctgctga ctttgatcca gccattgcca
    22501 taacgggcgg cgttgaccac catattcgcc accgcgcgtt tgatcgacag cgggtgcatt
    22561 ttcacttcaa tgctgccggg gtaaagcgcg gtttcaattt cccgctcata gccactttcg
    22621 gcagcaatca cctcaccgag cactgcatta agatccgcca tttccatcgg catctcctgc
    22681 ccggtgcgca ggtagtcgat aaactgctca atgatggcgt tgcactcttc gatatcttta
    22741 ttgatcgatt ctgccagata gccatcctgc tcgctcatca tctcagtcgc caggcgaata
    22801 cgcgtcagcg gcgtgcgcaa gtcgtgactt acccccgcca tcagcagcgt gcggtcatcc
    22861 gccagttgct taacaccagc cgccatatgg ttaaaggcac gggtaacgga acgcacctcc
    22921 gaagcgccat attcacgcag cggcggcgga ataatccctt taccaacctg caaggctgcg
    22981 tgttcgagat cgaccaacgg tcggttctgg atacgaataa acagccacgc cccgcctatc
    23041 gccaatagca taatcgccag cgtatagcgg aacagcggag agaaatcgcc ctgatgaatt
    23101 tcggtcagcg gcacgcgtac ccagatattg ggcgacagcc aggttttcag ccagacgaca
    23161 ggcgaacttt tgttgacctc aacgcgcact tccgtcgggc cgcccagttg ctgtgccatc
    23221 tgatggctta agaattcata gtgttgcgcc caacgcagac ctgcctcttc ggcagcctcg
    23281 ttggagtaga gagagatccc cagctcacgg tagatctccc gacggaaagc gggaggcaca
    23341 accaactgcg tgccgtcctc cagttgcagt ttgtcggtca tcaacatacg cacttcgtac
    23401 gcgaggactt tattaaactg ctggaggctc ggcaaaatcg cgaagttcag caccaccaga
    23461 taagtcgtca ccaggctggc gaacagcaag gtgacgatga gcaataacgt acgggcaaat
    23521 gaacttcgtg gcgagaagcg caatcgcctc atgctttaga gccgtccggt acaaagacgt
    23581 agcccaggcc ccaaacggtc tgaatgtaac gcggatgcgc tggatcttct tctaccatgc
    23641 ggcgcagacg cgaaatctgc acgtcgatgg agcgttccat tgcggaatat tcacgaccac
    23701 gggcaaggtt catcagctta tcgcgggaga gcggctcacg cggatggctg accagtgcct
    23761 tcagtaccgc aaactcaccg ctggtgagcg gcatcggctc atcttcgcgg aacatttcgc
    23821 gcgtaccgag gttaagtttg aacttaccga aagcaattac cgcctcttcc tgtgacggtg
    23881 cgcctggcag ttcgttcgcc tgacgacgca gcaccgcacg aatacgggcc agcagttcac
    23941 gcgggttaaa cggttttgga atgtagtcgt cagcgccaat ctccaggcct acgatacggt
    24001 ccacttcttc ccctttcgcc gtcaccataa tgatcggcat cgggttgctc tgactacgaa
    24061 gacgtcggca aatcgacaag ccatcttcac caggtaacat taaatccagt accataagat
    24121 ggaaagattc acgagtcagc aggcgatcca tctgttctgc attagcgacg cttcgaacct
    24181 ggaagccttg ttcggtgaga taacgttcca gcagcgcacg caggcgcatg tcgtcatcga
    24241 ccaccagaat cttgtagttc tcttgcattg tttgtactcc caaaggttcg caacaatttg
    24301 taagcgtgta ttcttaaaaa agctcacgtt cgtcaccagc taaatctggt atgaatttca
    24361 gcctaaattg ttacaaagca tattaaacag cagcttaagt atacaattta ttcggcgaaa
    24421 cattattgat tctgttgata tgatcacgtt atacccaatg tgcgcattat caaacagaca
    24481 aagggaatca acgagatgaa aacgcccctg gttacccggg aagggtatga aaaactcaaa
    24541 caagagctta attatctctg gcgtgaagaa cgcccggagg tcacaaaaaa ggtgacctgg
    24601 gccgcaagtc tgggcgaccg cagcgaaaat gctgactatc agtataataa aaagcgtctg
    24661 cgtgaaatcg accgtcgcgt gcgctatctc actaaatgcc tggaaaatct caaaatcgtc
    24721 gattactccc ctcagcagga aggcaaagtc ttttttggcg cgtgggtgga gattgaaaac
    24781 gacgatggcg tgactcaccg tttccgtatt gtcggctacg atgaaatttt tggccgtaaa
    24841 gattacatct ctatcgattc cccgatggcc cgcgcattgc tgaaaaaaga agtcggcgat
    24901 ctggcggtgg tgaatacccc tgccggggaa gcgaactggt atgttaatgc tatcgagtac
    24961 gtgaaaccgt aaggaagagt cttaacctcc tgccgatggc tggcattttt gccagccagt
    25021 ccgtataact atcccctgat ttttgatccg aaaagatgaa ctcaaaccat gatgaatgat
    25081 tcgttctgcc gcattattgc gggtgaaatt caggcgcgcc cggaacaggt tgacgctgcc
    25141 gttcgcctgc ttgacgaagg gaataccgtg ccgtttatcg cacgttatcg taaggaaatc
    25201 accggcggtc tggatgacac gcagctgcgt aatctggaaa cgcgtctgag ctatctgcgc
    25261 gagctggaag agagacgtca ggcgatcctc aaatccattt ccgagcaagg caaactcacc
    25321 gatgatctgg cgaaggccat caacgccacc ctaagcaaaa ccgaactcga agacctctac
    25381 ctgccctaca aacctaaacg ccgcacccgc gggcaaatcg ccattgaagc agggcttgag
    25441 ccgttggctg acctgctgtg gagcgatccg tcacacacgc cagaagtcgc cgctgcacaa
    25501 tatgttgatg ccgataaagg cgtggcagat accaaagccg cgctggatgg cgcgcgctat
    25561 atcctgatgg aacggtttgc cgaagatgcc acgctgctgg cgaaagtgcg tgattatctg
    25621 tggaagaacg cgcatttggt ttctacggtg gtgagcggta aagaagagga aggggcgaaa
    25681 ttccgcgact atttcgatca tcacgaaccg ttgtccacgg tgccttctca ccgcgcgctg
    25741 gcgatgttcc gtgggcgtaa cgaaggcgta ctccagcttt cgctgaatgc cgatccgcag
    25801 ttcgatgagc cgcccaaaga gagctattgc gagcaaatca tcatggatca ccttggcctg
    25861 cgcctgaaca atgccccggc ggatagctgg cgcaaaagcg tagtgagctg gacctggcgc
    25921 atcaaggtgc tgatgcatct ggaaaccgaa ctgatgggta ccgtgcgcga acgcgcggaa
    25981 gatgaagcaa tcaacgtctt tgcccgtaac ctgcacgatc tgctgatggc ggcccctgcc
    26041 ggactgcgtg caacgatggg cctcgatccg ggtctgcgta ccggggtaaa agtggcggtg
    26101 gtcgatgcca ctggcaaact ggtggcgacc gataccattt acccgcacac cggacaagcc
    26161 gcaaaagcag cgatgaccgt tgctgccttg tgtgaaaaac ataacgttga actggtggcg
    26221 atcggcaacg gtacagcttc ccgcgaaact gaacgtttct atctcgacgt gcagaagcag
    26281 ttcccgaaag tgaccgcaca gaaagtgatc gtcagcgaag ctggcgcgtc ggtttactca
    26341 gcttccgagc tggcagcgca ggagttcccg gatctcgacg tttcgctgcg tggtgcggta
    26401 tctatcgccc gccgtttgca ggatccgctg gcggagctgg tgaaaatcga tccgaaatct
    26461 atcggcgtag gtcagtatca gcatgacgtc agccagacgc aactggcccg caaactggat
    26521 gcagtagtag aagactgcgt aaacgccgtt ggcgtcgatc tcaacaccgc ttctgttccg
    26581 ctattaaccc gcgtggcggg cctgacgcgc atgatggcgc aaaacatcgt ggcctggcgc
    26641 gatgagaacg gccagttcca gaaccgtcag caactgctga aagtcagccg tctggggccg
    26701 aaagccttcg agcagtgcgc gggcttcctg cgcattaacc acggtgataa cccgctggac
    26761 gcctctaccg ttcatccgga agcctatccg gtggtggaac gcattctggc agcaacacag
    26821 caggcactga aagatctgat gggtaacagc agcgaactgc gtaacctgaa agcgtctgac
    26881 tttactgatg aaaaattcgg tgtgccgaca gtaactgaca tcatcaaaga gctggaaaaa
    26941 ccgggtcgcg atccgcgtcc ggaatttaaa accgctcagt ttgccgatgg cgtcgagaca
    27001 atgaacgacc tgcaaccggg tatgatcctc gaaggcgcag tgaccaacgt caccaacttt
    27061 ggcgcgtttg tcgatattgg cgtgcatcag gacggcctgg ttcacatctc ttcattgtcg
    27121 aacaagtttg tggaagatcc gcataccgtg gtgaaagcgg gcgacattgt gaaggtgaaa
    27181 gtgctggaag tggatcttca gcgtaaacgt atcgccctga ctatgcgtct ggacgagcag
    27241 cctggcgaaa ccaactctcg tcgcggcggc ggtaatgaac gcccgcaaaa caaccgcccg
    27301 gcagccaaac cacgcggtcg tgaagcgcag cctgccggta atagcgcgat gatggatgcg
    27361 ctggcggcag cgatgggtaa aaaacgttaa acgcccggac tggcccctaa acctgcgcga
    27421 taaggcgttc acgccgcatc tggcaacgaa cgctttgcct gacataaaag tgccggagaa
    27481 tatctccggc acttttattc cacagccaaa ctcacaatat actccgttaa tatttatcac
    27541 ttcattaaca actgaaacct taattaaaca ttagccagtc cgggtaactc actattcgaa
    27601 ttatattttc gctgcgatat aaccttgagc cacatcaaca ttgagtcaga ttattattca
    27661 aaccaacatt cgcacacatt ttaagtattg ctgatagaaa ccattctcat tatcattgtg
    27721 ttgttgatta tttaatctct cctttgttgg caaatcatct ggtctcatgt cgcggtcaaa
    27781 cgccccatga ggtagttatc cagttaatga gaaacaagta ggcacctatg caatacactc
    27841 cagatactgc gtggaaaatc actggctttt cccgtgaaat cagcccggca tatcgccaaa
    27901 aactgctttc tctaggcatg ttacctggct cctcttttga tgtggtgcgc gtcgctccac
    27961 tcggcgaccc cattcatatc gaaacccgtc gtgtgagcct ggtattacgc aaaaaagatc
    28021 tggccttatt agaagtggaa gcggtttcct gttaatacgg tgataacaac aatgaaaaaa
    28081 ttaaccattg gcttaattgg taatccaaat tctggcaaga caaccttatt taaccagctc
    28141 actggcgcac gtcagcgtgt aggtaactgg gctggcgtta ccgtcgaacg taaagaaggg
    28201 caattctcca ccaccgatca tcaggtcacg ctggtggacc tgcccggcac ctattctctg
    28261 accaccatct catcgcagac ctcgctcgat gagcaaatcg cctgtcacta cattttgagt
    28321 ggcgacgccg acctgctgat taacgtggtg gatgcgtcta accttgagcg taacctgtac
    28381 ctgacgctgc aactgctgga actcggcatt ccctgcatcg tggcgctgaa catgctcgac
    28441 attgccgaga agcaaaatat tcgtattgaa attgatgctc tatcggcacg tcttggctgc
    28501 ccggtgatcc cgctggtttc aacccgtggt cgcggtattg aagcgctcaa gctggcgatt
    28561 gatcgctata aagctaacga gaatgtggaa ctggtgcatt acgcacagcc gctactcaac
    28621 gaagcagatt cactggcaaa agtgatgcct tccgacatcc cgctgaaaca acgtcgctgg
    28681 ctgggcctgc aaatgctgga aggcgatatc tacagccgcg cctacgccgg tgaagcgtcg
    28741 cagcatctgg atgccaccct cgcccgtctg cgtaatgaaa tggatgatcc ggcgctgcac
    28801 attgccgatg cgcgttacca gtgcattgct gccatctgtg atgtggtaag caacaccctg
    28861 acggcagaac ccagccgttt caccactgcg gtagataaaa tcgtgctcaa ccgtttcctc
    28921 ggtctgccga ttttcctctt tgtgatgtac ctgatgttcc tgctggctat caacatcggc
    28981 ggggcgttac agccgctgtt taacgtcggc tccgtggcgc tatttgtgca tggtattcaa
    29041 tggattggct acacgctcca cttcccggac tggctgacta tcttcctcgc ccagggcctg
    29101 ggtggcggta ttaacaccgt gctgccactg gtgccgcaga tcggcatgat gtacctgttc
    29161 ctctccttcc ttgaggactc cggctatatg gcgcgtgcag cgtttgtgat ggaccgcctg
    29221 atgcaggcgc tgggcttgcc ggggaaatcc tttgtgccgc tgatcgtcgg tttcggttgt
    29281 aacgtaccgt cggtaatggg cgcacgtacg cttgatgcac cgcgtgaacg tctgatgacc
    29341 atcatgatgg caccgtttat gtcctgcggc gcgcgtctgg ctatcttcgc agtattcgcg
    29401 gctgccttct tcgggcagaa cggtgccctg gcggttttct cgctgtatat gctcggtatc
    29461 gtgatggcgg tgctgactgg cctgatgctc aagtacacca tcatgcgcgg tgaagcgacg
    29521 ccgtttgtca tggagctgcc ggtctaccat gtaccacacg ttaaaagcct gattatccag
    29581 acctggcagc gtctgaaggg cttcgttctg cgtgctggta aagtgattat catcgtcagc
    29641 attttcctga gcgctttcaa cagcttctcg ttgagcggga aaatcgtcga taacatcaac
    29701 gactcggcgc tggcttccgt cagccgggtg ataacccctg tcttcaagcc gattggcgta
    29761 catgaagata actggcaggc aacggttggc ctgtttacgg gcgcaatggc aaaagaagtg
    29821 gtggtgggta cgctcaacac cctctacacc gcagaaaata ttcaggacga agagttcaat
    29881 ccggcggaat tcaacctcgg tgaagagctg ttcagtgcgg tagatgaaac ctggcagagc
    29941 ctgaaagaca ccttcagcct tagcgtactg atgaacccca ttgaagctag caaaggcaac
    30001 ggtgaaatgg gtaccggggc gatgggcgtg atggatcaga aattcggtag cgcagctgcc
    30061 gcatacagct acctgatttt cgtcctgctg tatgtaccat gtatctcggt gatgggagcc
    30121 atcgcccgtg aatcaagccg tggctggatg ggcttctcca tcctgtgggg gctgaatatc
    30181 gcttactcac tggcaacatt gttctatcag gtcgccagct acagtcagca cccaacttac
    30241 agcctggtgt gcattctggc ggtaatcctg tttaacatcg tagctatcgg tctgctgcgc
    30301 cgcgcgcgta gccgggtgga tatcgaactg ctggcaaccc gcaagtcggt aagcagttgc
    30361 tgcgcagcca gcaccaccgg tgattgccat taatggcttc acttattcag gtacgtgatt
    30421 tgctggcgtt acggggccgt atggaagcga cccagataag ccagacattg aacactccgc
    30481 agccaatgat taacgccatg ctgcaacagc tggaaagcat gggcaaagcc gtgcggattc
    30541 aggaagaacc tgacggctgc ctctctggca gttgtaaaag ctgcccggaa ggcaaagcct
    30601 gtctgcgcga gtggtgggcg ctgcgttaac catactccat cgcctgtttt gaaaagcccg
    30661 gtatgcgttt gcatccgggc ttttttgcgt gcggctttcc ataaaaatgc aattcttgca
    30721 gcgcaacgta agttcctctg aaagcgtctc gcagggatga aaactcgcta atgcacagat
    30781 gtggagtggc gcgtagagtc gcggcattca aacaacaggt gaaggaacgc catgagtaaa
    30841 aagcagagtt ccaccccaca tgatgcgctg ttcaaactct ttttacgcca accggagacg
    30901 gcacgcgatt ttcttgcgtt tcatttaccg gcacccattc acgcgctctg tgatatgaaa
    30961 accctcaagc tggagtcgag gagctttatt gatgacgatc tgcgtgaaag ctattccgat
    31021 gtgctgtggt cggtgaaaac ggaacaagga ccaggataca tctattgtct gattgaacat
    31081 caaagcacct caaacaaact gatcgcattt cgcatgatgc gttacgctat tgccgcaatg
    31141 caaaatcacc ttgatgctgg atacaaaacg ttgccgatgg tggtgccatt gttgttttac
    31201 cacggtattg aaagccccta tccctattcg ctgtgttggc tggattgttt cgccgatccc
    31261 aaactggcaa ggcagcttta tgcctccgca tttccgctga ttgatgtcac cgtcatgcct
    31321 gatgatgaaa tcatgcagca ccgacgtatg gcgctgctgg agttaattca aaaacatatt
    31381 cgtcaacgcg atctgatggg gctggtagag caaatggcct gcttattaag tagtggatac
    31441 gctaatgaca gacaaatcaa agggctgttt aattacatac tgcaaactgg cgacgctgta
    31501 cgttttaacg attttatcga cggcgttgcc gaacgttcac cgaaacacaa ggagagttta
    31561 atgactattg cggaaagatt gcggcaggag ggggaacaat ccaaagccct gcatatagcc
    31621 aaaataatgc ttgaatccgg agtccctctt gcagacatca tgcgatttac cgggctgtca
    31681 gaagaagagt tggctgcggc gagtagactg gccccctgaa tctccagaca accag
//
LOCUS       DAZPAB010000024        31186 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZPAB010000024 DAZPAB010000000
VERSION     DAZPAB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.024"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            389..2047
                     /locus_tag="ACHUPG_001027"
     CDS             389..2047
                     /locus_tag="ACHUPG_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR0860735.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="ACHUPG_001028"
     CDS             complement(2044..3000)
                     /locus_tag="ACHUPG_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR0860736.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="ACHUPG_001029"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="ACHUPG_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR0860737.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="ACHUPG_001030"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="ACHUPG_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR0860738.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="ACHUPG_001031"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="ACHUPG_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR0860739.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6656..6730
                     /gene="yljB"
                     /locus_tag="ACHUPG_001032"
     CDS             6656..6730
                     /gene="yljB"
                     /locus_tag="ACHUPG_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR0860740.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="ACHUPG_001033"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="ACHUPG_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR0860741.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="ACHUPG_001034"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="ACHUPG_001034"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR0860742.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9859..9946)
                     /locus_tag="ACHUPG_001035"
     tRNA            complement(9859..9946)
                     /locus_tag="ACHUPG_001035"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Ser,seq:gga)
     gene            complement(10200..10418)
                     /gene="infA"
                     /locus_tag="ACHUPG_001036"
     CDS             complement(10200..10418)
                     /gene="infA"
                     /locus_tag="ACHUPG_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR0860743.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10703..11407)
                     /gene="aat"
                     /locus_tag="ACHUPG_001037"
     CDS             complement(10703..11407)
                     /gene="aat"
                     /locus_tag="ACHUPG_001037"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR0860744.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="ACHUPG_001038"
     CDS             complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="ACHUPG_001038"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR0860745.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="ACHUPG_001039"
     CDS             complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="ACHUPG_001039"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR0860746.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="ACHUPG_001040"
     CDS             complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="ACHUPG_001040"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR0860747.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16569..17063
                     /gene="lrp"
                     /locus_tag="ACHUPG_001041"
     CDS             16569..17063
                     /gene="lrp"
                     /locus_tag="ACHUPG_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR0860748.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17198..21226
                     /gene="ftsK"
                     /locus_tag="ACHUPG_001042"
     CDS             17198..21226
                     /gene="ftsK"
                     /locus_tag="ACHUPG_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR0860749.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21381..21992
                     /gene="lolA"
                     /locus_tag="ACHUPG_001043"
     CDS             21381..21992
                     /gene="lolA"
                     /locus_tag="ACHUPG_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR0860750.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22003..23346
                     /gene="rarA"
                     /locus_tag="ACHUPG_001044"
                     /gene_synonym="mgsA"
     CDS             22003..23346
                     /gene="rarA"
                     /locus_tag="ACHUPG_001044"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR0860751.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23437..24729
                     /gene="serS"
                     /locus_tag="ACHUPG_001045"
     CDS             23437..24729
                     /gene="serS"
                     /locus_tag="ACHUPG_001045"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR0860752.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24969..27412
                     /gene="dmsA"
                     /locus_tag="ACHUPG_001046"
                     /pseudo
     CDS             24969..27412
                     /gene="dmsA"
                     /locus_tag="ACHUPG_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27423..28040
                     /gene="dmsB"
                     /locus_tag="ACHUPG_001047"
     CDS             27423..28040
                     /gene="dmsB"
                     /locus_tag="ACHUPG_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR0860753.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28042..28833
                     /locus_tag="ACHUPG_001048"
     CDS             28042..28833
                     /locus_tag="ACHUPG_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009390 - dimethyl sulfoxide reductase
                     complex [Evidence IEA];
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HFR0860754.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28869..29495)
                     /gene="ycaC"
                     /locus_tag="ACHUPG_001049"
     CDS             complement(28869..29495)
                     /gene="ycaC"
                     /locus_tag="ACHUPG_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR0860755.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29810..30958
                     /locus_tag="ACHUPG_001050"
     CDS             29810..30958
                     /locus_tag="ACHUPG_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0860756.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            30976..>31186
                     /locus_tag="ACHUPG_001051"
     CDS             30976..>31186
                     /locus_tag="ACHUPG_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0860757.1"
                     /translation="MNLLFHTLLFCFFYLTCFFPNFIRLKIKCAAGYNFAFRYFRTSY
                     LPRIIPRFTDAPLILAKDLSMAGNVQ"
BASE COUNT         7689 a         7644 c         8374 g         7479 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgt cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcaggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
     9721 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9781 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9841 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9901 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9961 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
    10021 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10081 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10141 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10201 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10261 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10321 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10381 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10441 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10501 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10561 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10621 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10681 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10741 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10801 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10861 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10921 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10981 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    11041 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11101 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11161 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11221 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    11281 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11341 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11401 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11461 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11521 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11581 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11641 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11701 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11761 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11821 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11881 cagtgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11941 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    12001 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12061 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12121 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12181 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12241 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12301 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12361 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12421 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12481 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12541 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12601 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12661 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12721 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12781 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12841 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12901 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12961 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    13021 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13081 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13141 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13201 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13261 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13321 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13381 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13441 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13501 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13561 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13621 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13681 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13741 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13801 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13861 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13921 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13981 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    14041 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14101 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14161 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14221 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14281 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14341 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14401 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14461 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14521 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14581 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14641 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14701 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14761 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14821 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14881 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14941 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    15001 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15061 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15121 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15181 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15241 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15301 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15361 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15421 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15481 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15541 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15601 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15661 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15721 ccgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15781 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15841 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15901 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15961 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    16021 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16081 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16141 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16201 taaatgatga ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg
    16261 ttttgctttg acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16321 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16381 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16441 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16501 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16561 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16621 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16681 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16741 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16801 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16861 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16921 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16981 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    17041 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17101 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17161 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17221 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17281 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17341 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17401 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17461 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17521 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17581 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17641 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17701 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17761 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17821 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17881 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17941 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    18001 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18061 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18121 tccattatta aacagtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18181 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18241 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18301 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18361 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18421 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18481 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18541 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18601 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18661 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18721 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18781 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18841 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt
    18901 tgccgcgcca gtcttcagtc tggcaaatag cggtggaccg cgtccgcagg tcaaagaggg
    18961 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    19021 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19081 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19141 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19201 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19261 tcagtttgcg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19321 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19381 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc
    19441 acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagcagcagt atcagcagcc
    19501 acaacagcag gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca
    19561 gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca
    19621 acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc
    19681 gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa
    19741 accgacgacg ccactgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc
    19801 ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt
    19861 ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact
    19921 taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg
    19981 ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg
    20041 tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc
    20101 caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga
    20161 gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc
    20221 cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga
    20281 agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat
    20341 tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg
    20401 gtgtgttaac gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct
    20461 ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc
    20521 gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata
    20581 cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga
    20641 agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc
    20701 aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg
    20761 tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc
    20821 ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt
    20881 acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa
    20941 agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg
    21001 tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt
    21061 tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta
    21121 taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg
    21181 gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg
    21241 gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc
    21301 tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg
    21361 acgtaatttg aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct
    21421 tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag
    21481 tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc
    21541 aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac
    21601 aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg
    21661 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga
    21721 ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact
    21781 ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac
    21841 gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc
    21901 agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc
    21961 aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc
    22021 gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca
    22081 cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa
    22141 gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc
    22201 gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct
    22261 ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc
    22321 cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt
    22381 ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg
    22441 tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt
    22501 acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt
    22561 ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc
    22621 gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat
    22681 agcggtaagc gggtcctgaa gcctgaatta ctgaccgaaa tcgccggtga acgtagcgcc
    22741 cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta
    22801 cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc
    22861 gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc
    22921 gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg
    22981 gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc
    23041 aacgctgtct acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg cccggattat
    23101 gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg
    23161 caggaatatc gttatgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc
    23221 ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag
    23281 attggtgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac
    23341 cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc
    23401 acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt
    23461 aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat
    23521 aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa
    23581 gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc
    23641 gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag
    23701 ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca
    23761 gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggtacc
    23821 ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat gcactctggc
    23881 ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag
    23941 attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat
    24001 ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt
    24061 cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac
    24121 accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt
    24181 gaaatcatcg atgaagatga tctgccaatt aagatgaccg cccacacccc atgcttccgt
    24241 tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac
    24301 aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa tggcggcgct ggaagagatg
    24361 actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt
    24421 tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg
    24481 gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt
    24541 cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg
    24601 aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag
    24661 gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat
    24721 attggctaat acccaatttt tctgaatcta aaaagcgcct ttagggcgct ttttttgtct
    24781 ccctttgata ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa
    24841 atctacctat ctctttgatt ttcaaattat tctatgtata caagcctata tagcgagctg
    24901 ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgatc aagcttgagt
    24961 gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggag agtcgccgtg
    25021 gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac
    25081 cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg
    25141 ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg
    25201 tggacggtga aatcaaatat gtcgaaacga caataccggc gatgacaatt acgacggcct
    25261 acactaggtt cgcgcctgcc tgcgtgggcg ttccatgcgt caccgtgtct acaatccgga
    25321 ccgcctgaaa tatccgatga aacgagtcgg ggcgcgcggt gaaggcaaat tcgagcgcat
    25381 tagctgggaa gaagcctacg acatcatcgc gaccaatatg cagcgcctga tcaaagagta
    25441 cggcaacgag tctatctatc tgaactatgg caccggtacg ctgggcggca ccatgacccg
    25501 ctcctggccg ccgggaaata ccctggtcgc gcgcctgatg aactgctgcg gcggttatct
    25561 taaccattac ggtgactact cttccgcgca aattgcggaa ggtttgaacc atacctacgg
    25621 cggctgggca gatggtaaca gcccgtcgga tatcgaaaac agtaaattgg tagtgctgtt
    25681 tggtaataac ccaggcgaaa cgcgaatgag tggcggtggg gtgacttact atcttgaaca
    25741 ggcacgccag aaatctaatg ctcgcatgat catcatcgat ccgcgctata ccgacaccgg
    25801 tgccgggcgc gaagatgagt ggatccctat tcgtccagga acagatgccg cactggttaa
    25861 cggtctggcg tacgtcatga tcactaaaaa cctggtggat caggcattcc tcgataaata
    25921 ttgcgttggc tatgacgaga aaaccctgcc cgccagtgcg ccgaaaaatg gtcattataa
    25981 agcttatatt ctgggtgaag ggccagatgg cgtggctaaa acaccggaat gggcctcgca
    26041 aatcaccggt gttccggcag acaaaatcat caaactggct cgtgaaatcg gcagtaccaa
    26101 accggtgttt atcagccagg gatggggccc gcagcgtcac gctaacggtg aaatcgcaac
    26161 ccgtgctatc tcgatgctgg cgattctgac cggtaacgtt ggtattaatg gagggaacag
    26221 cggcgcgcgt gaaggttcat acagcttacc gtttgtccgt atgccgacct tggaaaaccc
    26281 gatccagacc agcatttcga tgtttatgtg gaccgatgcc attgaacgtg gcccggaaat
    26341 gacggcgctg cgtgatggtg tgcgcgggaa agataagctg gatgtgccga tcaaaatgat
    26401 ctggaactat gccggtaact gcctgattaa ccagcattct gaaatcaacc gtacccatga
    26461 aatcctccag gatgataaga agtgcgagct gattgtggtt atcgactgcc acatgacctc
    26521 atcggcgaaa tatgctgaca tcctgctgcc tgactgcacc gcttccgaac agatggactt
    26581 tgcactggat gcatcctgcg ggaatatgtc ttacgtgatt ttcaacgatc aggtgattaa
    26641 accgcgcttt gaatgtaaga ccatctatga aatgaccagc gaactggcaa aacgtcttgg
    26701 cgttgagcaa cagtttactg aaggccgtac ccaggaagag tggatgcggc atctgtatgc
    26761 ccagtcgcgg gaagcgattc ctgaactgcc aacgtttgaa gagttccgca agcaggggat
    26821 ctttaaaaag cgcgacccac aagggcatca cgttgcttat aaagccttcc gtgaagatcc
    26881 gcaggcaaac ccactgacta cgccatcggg caaaattgag atttattcgc aggcgctggc
    26941 tgacattgcc gctacctggg aattgccaga aggcgatgtg atcgatccac tgccgatcta
    27001 cacgccgggc tttgaaagtt atcaggatcc gctgaacaaa cagtatccgc tgcagcttac
    27061 aggtttccac tataaatctc gcgttcactc aacttacggc aacgttgatg tgctgaaagc
    27121 ggcttgccgt caggaaatgt ggatcaaccc gcttgatgcc caaaaacgcg gtatccacaa
    27181 cggcgataaa gtcaggatct ttaacgatcg tggtgaggtt catattgagg cgaaagtgac
    27241 gccacgaatg atgccgggcg tggtcgcact gggtgaaggc gcctggtatg acccggatgc
    27301 aaaacgtgtc gataagggcg gttgtatcaa cgtactgacc actcaacgtc cgtctcctct
    27361 cgctaagggg aatccgtcac atacaaacct tgttcaggtt gaaaaggtgt aaggagtaac
    27421 cgatgacaac ccagtatgga ttttttattg attccagccg ttgcaccggt tgcaaaacct
    27481 gcgagctggc ttgtaaagac tacaaagatt tgacgccaga agtcagtttc cgccgcattt
    27541 atgaatatgc tggcggcgac tggcaggaag ataacggtgt ctggcaccag aacgtgtttg
    27601 cctactatct gtcgatttcg tgcaaccact gcgaagatcc ggcttgtact aaagtctgcc
    27661 cgagcggtgc gatgcataaa cgcgaagatg gttttgttgt ggtcgatgaa gatgtatgca
    27721 ttggctgccg ttactgtcat atggcttgcc cgtatggcgc accgcaatat aacgaaacga
    27781 aaggccatat gaccaaatgc gatggttgtt atgaccgtgt tgcggagggt aaaaagccga
    27841 tctgtgttga atcttgcccg ctgcgggcgc tggatttcgg ccctatcgac gagctgcgta
    27901 aaaaacatgg cgatctggcg gccgttgcgc cgttgccgcg agctcacttt accaaaccga
    27961 atattgtgat caaacccaat gccaatagcc gcccgaccgg ggataccact ggctatctgg
    28021 caaacccgaa ggaggtgtga gatgggaagt ggatggcatg aatggccgct gatgatcttc
    28081 acggtcttcg ggcaatgtgt ggcgggtggt tttatcgttc tggctttggc gctgctcaaa
    28141 ggcgacctgc gagcagaagc ccagcagcgt gttatcgcct gcatgtttgg cttatgggta
    28201 ctgatgggca ttggctttat cgcctctatg ctccatcttg gttcaccgat gcgcgccttt
    28261 aactcgctca accgggtagg ggcttcagca ctcagtaacg aaatcgccag cggttcgatc
    28321 ttttttgctg taggcggcat cggctggttg ctggcaatgc tgaaaaagct gtcaccggca
    28381 ttgcgtacgc tatggctggt ggtgacgatg gttcttggcg tcgtctttgt ctggatgatg
    28441 gtgcgtgtgt ataacagcat tgataccgtt ccgacctggt acagcatctg gacgccgatg
    28501 ggctgggcga tgcgtctgct gccagccatt tctgtactgg cactggtggt gagtggcgtg
    28561 atgtcagtga tgcagggcac agagctggca actattcata gttctgtgca gcaggccgca
    28621 gcgctggtgc cggactatgg tgcgctgatg tcctggcgga tcgtgctttt ggccgttgcc
    28681 ctgtgcttgt ggattgcacc acagctaaaa ggttatcagc ctgcggttcc gctactatcc
    28741 gtctcattca ttctgcttct ggcaggggaa ttaatcggtc gcggcgtatt ctatggtttg
    28801 catatgaccg tggggatggc cgtcgcaagc taatcagtaa caaccggggt ttcggccccg
    28861 gttctctttt atttctgctt cgttaacgtg tcataactgg tcatcaaatt acgataatca
    28921 ggaatatggt tagagaacaa cgtcgccagt ccttcaatat cattacgcca gtcacggtgc
    28981 agctcgcaag ccacgccgaa ccatgtcatc aattgcgcac cagcttgcga catgcgatcc
    29041 cacgccgaat gccgggtaat ttcattaaag gtccctgatg catcagtcac gacaaagaca
    29101 tcaaaccctt cttcaatggc tgaaagcgcc gggaatgcca cgcaaacttc ggttaccaca
    29161 ccggcaataa ttaactgttt tttacctgtc gctttgactg cttttacaaa atcttcgtta
    29221 tcccaggcgt taatattgcc cgggcgagca atgtaaggtg catcggggaa ttgtgctttc
    29281 agttctagaa ctaatgggcc gttaggaccg gtttcaaaac tggtggtaag aatagttggt
    29341 aagttgaaat acttagccag gtcacccagc gccagcacgt tatttttaaa cttatcgggt
    29401 tcgatatccc gtacaaggga aagtaaacca gcctggtgat caacaagcaa aacggcagca
    29461 tcatttttat caagacgaac atacggtttg gtcatcctct tctcctttcg agagtcagtg
    29521 gtacgtctga aatcggtcag acgcgatgca ttgctctgaa agcatagacg ggaaatatga
    29581 gtttgctgtg accatgaaat ttttcgactg aacgaagcgt atgaaatttg tgttagatca
    29641 ataaaaacaa tcagatgaga ttaatcatct cttcagcatt agcgttatga atgagtaata
    29701 taaatcgata ataccgccgc tgcgatatgc gcgtattttc acccattgac aatgtttttg
    29761 gcggtggcat gatgcgcatg aaatttgaac ttcctcacgg ttttaattca tgtccacgta
    29821 tacccggcct gtcatgcttt tgctgtctgg cctgcttttg ttgactctgg cgatcgcggt
    29881 gttaaataca ctcgtgccgc tttggctcgc ccaggaacac atgtccacat ggcaggtagg
    29941 cgttgtcagc tcatcctatt ttaccggcaa ccttgtcggt acattgctga cagggtatgt
    30001 cattaaacgc attggcttta accgcagcta ttatctgtcc tccttcattt ttgctgctgg
    30061 ctgtgccggc cttggcctga tgattggttt ctggagctgg ttggcgtggc gttttgtcgc
    30121 gggcgtcggc tgtgccatga tttgggtggt tgttgagagc gcgctgatgt gcagtgggac
    30181 gtcacgtaac cgtgggcgtt tgcttgctgc gtatatgatg gtttattacg tgggaacgtt
    30241 tttaggccag ttactggtca gcaaagtctc aaccgagctg atgagcgtat tgccgtgggt
    30301 tacaggtttg acattggcag ggatcttacc gctgttgttt acgcgtgtcc tgaatcagca
    30361 ggctgaaaac catgattcga cgtcaattac ggcaatgcta aaactccgtc aggcgcggct
    30421 tggcgtgaat ggctgcatta tctcaggaat cgttctggga tctctatatg gcctgatgcc
    30481 tctgtacctc aatcacaaag gggtgagcaa tgccagtatt ggtttctgga tggcggtact
    30541 ggtcagtgcg ggtatcctcg gacaatggcc gattggacgt ctggcggata agtttggtcg
    30601 actgctggtt ttgcgtgttc aggtctttgt cgtcattctc ggcagtattg cgatgcttag
    30661 ccaggcggcg atggccccag cgttattcat cctcggtgcc gctggcttta cgctatatcc
    30721 ggtggcgatg gcctgggctt gtgagaaagt tgaacatcat caactggtgg cgatgaacca
    30781 agccttactg ttgagctata ccgtgggaag tctgcttggc ccgtcattta gcgctatgct
    30841 aatgcagaat ttctccgata atttattgtt tatcatgatc gccagcgtat cgtttatcta
    30901 tttgctgatg ctgcttcgca acgccggtca tacgccgaaa cccgttgctc acgtgtaaat
    30961 gaattcaagc aaagtgtgaa cttactgttt cacactctgc ttttttgttt cttctatctg
    31021 acttgctttt ttccaaattt tattcgttta aaaataaaat gtgcagcagg ttataatttt
    31081 gcatttcgct atttccgcac ttcttatttg ccgcgcataa tccctcgttt tacagatgcc
    31141 cctttaattt tggcgaagga tttgtctatg gctgggaatg ttcagg
//
LOCUS       DAZPAB010000025        30804 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZPAB010000025 DAZPAB010000000
VERSION     DAZPAB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.025"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..1150)
                     /gene="setB"
                     /locus_tag="ACHUPG_001052"
     CDS             complement(<1..1150)
                     /gene="setB"
                     /locus_tag="ACHUPG_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR0860758.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIAT"
     gene            1518..2648
                     /gene="fruB"
                     /locus_tag="ACHUPG_001053"
     CDS             1518..2648
                     /gene="fruB"
                     /locus_tag="ACHUPG_001053"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR0860759.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2648..3586
                     /gene="fruK"
                     /locus_tag="ACHUPG_001054"
     CDS             2648..3586
                     /gene="fruK"
                     /locus_tag="ACHUPG_001054"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR0860760.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3603..5294
                     /gene="fruA"
                     /locus_tag="ACHUPG_001055"
     CDS             3603..5294
                     /gene="fruA"
                     /locus_tag="ACHUPG_001055"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR0860761.1"
                     /translation="MKTLLIIDANLGQARAYMVKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5718..6659
                     /locus_tag="ACHUPG_001056"
     CDS             5718..6659
                     /locus_tag="ACHUPG_001056"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR0860762.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6647..7585
                     /gene="psuG"
                     /locus_tag="ACHUPG_001057"
     CDS             6647..7585
                     /gene="psuG"
                     /locus_tag="ACHUPG_001057"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR0860763.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7679..8929
                     /locus_tag="ACHUPG_001058"
     CDS             7679..8929
                     /locus_tag="ACHUPG_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR0860764.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9000..9694)
                     /locus_tag="ACHUPG_001059"
                     /pseudo
     CDS             complement(9000..9694)
                     /locus_tag="ACHUPG_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9825..10766
                     /gene="rihB"
                     /locus_tag="ACHUPG_001060"
     CDS             9825..10766
                     /gene="rihB"
                     /locus_tag="ACHUPG_001060"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR0860765.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10866..12116
                     /locus_tag="ACHUPG_001061"
     CDS             10866..12116
                     /locus_tag="ACHUPG_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR0860766.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12172..13260)
                     /locus_tag="ACHUPG_001062"
     CDS             complement(12172..13260)
                     /locus_tag="ACHUPG_001062"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR0860767.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="ACHUPG_001063"
     CDS             complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="ACHUPG_001063"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR0860768.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14194..15243)
                     /locus_tag="ACHUPG_001064"
     CDS             complement(14194..15243)
                     /locus_tag="ACHUPG_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR0860769.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15342..16223
                     /gene="yieE"
                     /locus_tag="ACHUPG_001065"
     CDS             15342..16223
                     /gene="yieE"
                     /locus_tag="ACHUPG_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR0860770.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16429..17898
                     /gene="lysP"
                     /locus_tag="ACHUPG_001066"
     CDS             16429..17898
                     /gene="lysP"
                     /locus_tag="ACHUPG_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR0860771.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18190..20181
                     /gene="cirA"
                     /locus_tag="ACHUPG_001067"
                     /pseudo
     CDS             18190..20181
                     /gene="cirA"
                     /locus_tag="ACHUPG_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="ACHUPG_001068"
                     /pseudo
     CDS             complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="ACHUPG_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21306..21974
                     /gene="folE"
                     /locus_tag="ACHUPG_001069"
     CDS             21306..21974
                     /gene="folE"
                     /locus_tag="ACHUPG_001069"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR0860772.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21991..23067
                     /gene="yeiB"
                     /locus_tag="ACHUPG_001070"
     CDS             21991..23067
                     /gene="yeiB"
                     /locus_tag="ACHUPG_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR0860773.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23209..24249
                     /gene="galS"
                     /locus_tag="ACHUPG_001071"
     CDS             23209..24249
                     /gene="galS"
                     /locus_tag="ACHUPG_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR0860774.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24529..25527
                     /gene="mglB"
                     /locus_tag="ACHUPG_001072"
     CDS             24529..25527
                     /gene="mglB"
                     /locus_tag="ACHUPG_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR0860775.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25588..27108
                     /gene="mglA"
                     /locus_tag="ACHUPG_001073"
     CDS             25588..27108
                     /gene="mglA"
                     /locus_tag="ACHUPG_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR0860776.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27124..28134
                     /gene="mglC"
                     /locus_tag="ACHUPG_001074"
     CDS             27124..28134
                     /gene="mglC"
                     /locus_tag="ACHUPG_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR0860777.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28170..29383)
                     /gene="preA"
                     /locus_tag="ACHUPG_001075"
                     /pseudo
     CDS             complement(28170..29383)
                     /gene="preA"
                     /locus_tag="ACHUPG_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29377..30615)
                     /gene="preT"
                     /locus_tag="ACHUPG_001076"
     CDS             complement(29377..30615)
                     /gene="preT"
                     /locus_tag="ACHUPG_001076"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFR0860778.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
BASE COUNT         7478 a         7477 c         8157 g         7692 t
ORIGIN      
        1 gagtggcgat aatcatcacc atcgcaaacc agaacacagc gtgataattc cagatctcgg
       61 cgacgatgcc cgccactgat cctgcgatga tccagcccac gcgcgacgtg ttggtataga
      121 gcgtggtggc tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc atcccgatgc
      181 cgcccagaat gccaataaaa atagcattta gcagctgcaa gcccaacaga atggcaggtg
      241 aatgcgccat cagcattcct gcgtaaaaac agacgccacc cacggcagca acgcgcatta
      301 agaaacgctt acccagacgt ttggcgaaat atccggcaat caacatcgtc gggatttcca
      361 gcccggcggc ggtccccatc atcacaccgg ccagtttctc gggaagatgc agttcgttga
      421 taataaatag cggcatgttg atgatgtaca ggctgttcga gccccacatc aatgtacaaa
      481 tgacaaacag cagtagcgta tcgcgacggt tacggcgcgg cgcttcaacc gtgccggtcg
      541 ccagcggaag ctctttttgc atcgacggta aaaacagcca caccatcacg ccgcaaacaa
      601 taaacgctac cgctgcgctc agatacatta ccgtaaagct gaaacccatc gctaacgcat
      661 aagccagcgg tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa aaagagctga
      721 acatcaccgc ctcacgtccg gttttgtcgg catgttcacg ggcaagggca aacatttgcg
      781 ggttagcggt cgagccaaag ctgctaagaa agacgccaac gaatagcaaa acaaagtagt
      841 tgcgattcca agcaaaaagg gtgcaggcca gcacgcctaa caggcagcaa aagacaatca
      901 gcgatttgcg atcgccgcgc ttatcagagc gcccagcgag aaactgactt accagaatcc
      961 caatgacagc gctgccggta aagaagaatc ccaccatcgc cggacgggca tgtacttcat
     1021 cggtaaggaa aatactgagt gtcggggttt gcagagcacc cgcaataccg gtgagaaagg
     1081 caacgattaa aaacgccgtc gaggtcaggt caaacgattt cgcgctggag actgcggggg
     1141 agttatgcat gctggtcgat cactggaaga ggaagacgcg gagtttacgc tgcttgtcag
     1201 aaaataaaca gacagcgaaa tcaaaagcgc cacgaataat caaaatgcca tttcactgtt
     1261 gctgattggc tgctgacgct gctgaaatga aattgctgat aacggatttt cccatcagca
     1321 attaggaaaa atgacaaaaa attgtgcagc acatcaaact tttgctcata actttacggc
     1381 tttccttgcg tgctgaaaca gaagtattat gctttcttga aacgtttcag cgcgatcttg
     1441 tctttaaccc taagctaggt tggcgctttt tttctcatag aggctgaatc gtttcaattc
     1501 agcaagagag gagaactatg ttccagttat ccgtacagga catccatccg ggcgaaaagg
     1561 ccggagacaa agaagaggcg attcgccagg tcgctgcggc gctggtgcag gccggtaatg
     1621 tagcagaagg ctacgtcaat ggcatgctgg cgcgcgaaca gcaaacctca acgttcctcg
     1681 gcaatggtat tgctattcca cacggcacta ccgacacccg cgatcaggtg ctgaaaaccg
     1741 gcgttcaggt atttcagttc ccggaaggcg tcacctgggg tgacggtcag gtagcgtacg
     1801 tggcaatcgg tattgctgcc agctcggata aacatctggg cctgctacgc cagctgaccc
     1861 acgtactgag cgatgattcc gttgctgaac aactgaagtc agcaacaaca gcagaagaac
     1921 ttcgcgcatt actgatgggc gaaaagcaga gtgagcagct gaagctcgac aacgaaatgc
     1981 tgacgctgga tatcgtcgcc agcgatctgc tgactcttca ggcgctgaac gctgcgcgtc
     2041 tgaaagaggc gagggcagtt gacgccactt tcgtcaccaa agccatcaat gaacaaccgc
     2101 tgaatctcgg acagggtatc tggctgagcg atagcgccga aggcaatctg cgtagtgcga
     2161 ttgcggtaag ccgtgcggca aatgcttttg atgtggacgg cgaaacggca gccatgctgg
     2221 tgagtgtggc gatgaatgac gatcagccca tcgcggttct taagcgtctc gctgatttgt
     2281 tgctcgacaa taaagctgac cgcttgctga aagcggatgc ggcaacgttg ctggcgctgc
     2341 tgaccagcga tgatgcgccg accgacgacg tgttaagcgc ggagtttgtg gtgcgcaatg
     2401 aacacggcct gcatgctcgt ccaggtacca tgctggtcaa taccattaaa caatttaaca
     2461 gtgatattac cgtgacaaac cttgatggca ccggcaaacc ggcaaacgga cgtagtctga
     2521 tgaaagttgt ggcacttggc gttaagaaag gtcatcgcct acgctttacc gcccagggtg
     2581 cagatgctga acaggcgctg aaagccattg gcgacgctat cgctgctggt cttggggagg
     2641 gcgcataatg agcagacgtg ttgctacgat cacccttaat ccggcttatg accttgttgg
     2701 tttctgcccg gaaattgaac gcggcgaagt gaacctggtg aaaaccaccg gtctgcatgc
     2761 ggcgggtaaa ggcatcaacg tggccaaagt attaaaagac ctgggaattg atgtcaccgt
     2821 tggcggcttc ctgggtaaag acaatcagga tggttttcag caactgttca gcgagctggg
     2881 cattgccaac cgtttccagg ttgtacaggg gcgcacccga attaacgtta agctgacgga
     2941 aaaagacggc gaagtgaccg acttcaactt ctcgggtttc gaagtcaccc ccgccgactg
     3001 ggaacgcttt gtgactgatt ctctgagctg gctcggtcag ttcgatatgg tctgtgtcag
     3061 cggaagctta ccgtcaggcg tcagcccgga agcgttcacc gactggatga ctcgcctgcg
     3121 tagtcagtgt ccttgcatta tctttgatag tagccgtgaa gcgttagtag caggtttgaa
     3181 agcggcaccg tggctggtga aacctaaccg ccgcgagttg gaaatctggg caggccgtaa
     3241 actgcctgaa atgaaagatg tgattgaagc tgcacatgcg ctacgtgaac aaggtattgc
     3301 gcatgttgtt atttcactgg gtgccgaagg cgcgctttgg gttaatgcct ccggcgaatg
     3361 gatcgctaaa ccaccgtcag tcgatgtcgt aagcaccgtt ggcgcagggg attctatggt
     3421 tggtggcctg atttatggct tgctgatgcg tgaatccagt gaacacacac tgcgtctggc
     3481 gacagctgtt gcagccctgg cggtaagtca aagcaatgtg ggtattaccg atcgtccgca
     3541 gttggccgca atgatggcgc gcgtcgactt acaacctttt aactgacagc aggagaggca
     3601 taatgaaaac gctgctgatt attgacgcta atctcggtca ggcacgcgcc tatatggtga
     3661 agaccctgct gggcgcggcg gcgcgaaaag caaaactgga aatcatcgac aatccgaacg
     3721 acgcagaaat ggcgattgtt ctcggtgatt ccatcccgaa tgacagcgcg ctgaacggta
     3781 aaaatgtctg gctgggtgat atttcccggg cagttgcgca ccctgagctg ttcctgagtg
     3841 aagccaaagg ccatgcgaaa ccttacactg cgccggtcac tgcgacagca ccggttgccg
     3901 ccagcggtcc gaaacgcgta gttgcggtga ctgcttgccc gactggcgta gcacacacct
     3961 ttatggcggc tgaagccatt gaaaccgaag cgaaaaaacg tggctggtgg gtgaaagttg
     4021 aaacccgtgg ttctgttggc gcgggtaatg caatcactcc cgaggaagta gccgcagcgg
     4081 atctggtgat tgtggcggca gatatcgaag tggatctggc gaaatttgct ggtaaaccga
     4141 tgtatcgtac ctctaccggt ctggcgctga agaaaacctc gcaggaactg gataaagcgg
     4201 ttgctgaagc aacgccgtat gaaccggcgg gcaaagctca aacggcgacc actgaaggta
     4261 agaaagagag tgcaggcgct tatcgtcact tgctaacggg cgtctcttac atgctgccga
     4321 tggtcgttgc tggtggtctg tgtatcgcgc tttcttttgc ttttggtatc gaagcgttta
     4381 aagagccggg tacgttggca gcggcgctga tgcagattgg tggtggttca gcctttgcgc
     4441 tgatggtgcc ggtactggca ggttatattg ccttctccat tgccgatcgt ccgggcctca
     4501 cgccgggtct gattggcggt atgctggcgg tcagcaccgg ttctggcttc attggcggta
     4561 ttattgcggg cttcctggct ggttacattg cgaagttaat cagtacgcaa ctgaaactgc
     4621 cgcagagtat ggaggcgctg aaaccgatcc tgatcattcc gctaatttcc agtctggtgg
     4681 tcggtctggc gatgatctac ctgatcggta aaccagttgc tggcattctc gaagggctga
     4741 ctcactggct gcagaccatg gggactgcga atgcggttct gctgggggcg atcctcggtg
     4801 gcatgatgtg tactgacatg ggcggtccgg taaacaaagc agcgtacgca ttcggtgtgg
     4861 gtctgctgag tactcaaacc tatggcccga tggcggcgat tatggcggca ggtatggtgc
     4921 caccgctggc aatgggcctg gcaacaatgg tggcgcgtcg caaattcgac aaagcgcagc
     4981 aggaaggtgg caaagccgct ctggtattgg gactgtgctt catttcggaa ggtgcaattc
     5041 cgtttgctgc tcgtgatccg atgcgtgtgc tgccgtgctg tatcgtgggt ggggcgctga
     5101 ctggcgcaat ctcaatggcg attggtgcga aactgatggc accgcacggt ggtctgtttg
     5161 ttctgctgat ccctggcgct attacgccgg tattgggtta cctggtagca attattgccg
     5221 gtacgctggt ggcgggtttg gcctatgcct tcctgaaacg tccggaagtg gacgcagtag
     5281 cgaaagcagc gtaataaaag gtgtgttaca gggcagaaat ttcctgccct gtaacatttg
     5341 atgagcgcaa agagaatgac cagcttgtga atgtaatttt atgaatttaa atggttttat
     5401 ctttcagtag aaacttcaaa cgttcatcaa acactaaggt tatttcctca agggcagatt
     5461 attttctgcc cttttttagt gttcctgttc tgtgcatccc gtcacaaatt cctcgctttt
     5521 gtaattcagg catttaatcg cattttgtga gcctgctcaa atctgcccat tttgcctctc
     5581 tggcggtaat ttactttacg taaaacatgc atactatgag cacatgttta aatatgaaca
     5641 catgtttaaa ccgtctttgc agtatctata ttcttgtgga ttgacagggt tggtaatttt
     5701 ttaccagagg acgtgctatg cgcgaaaagg attatgtcgt aattataggt tcggcgaata
     5761 ttgatgtcgc cggatattca catgaatcat taaattatgc ggattcaaat ccaggtaaaa
     5821 taaaatttac gcctggtgga gtagggcgca atattgcaca aaacctggcg ttgctgggta
     5881 acaaagcctg gctactgagc gccgtaggca gtgattttta tggtcaatcg ctgctaacgc
     5941 aaaccaatca atctggtgtt tatgtcgata aatgcctgat tgtgccggga gaaaatacgt
     6001 cgagttattt atcattactc gataataccg gtgaaatgct ggttgctata aatgacatga
     6061 atattagcaa cgctattaca gctgaatatc tcgcacagca ccgtgaattt attcagaggg
     6121 caaaggtcat tgtcgcggac tgtaatatca gtgaagaggc actggcatgg attctggata
     6181 atgccgccaa cgtacccgta tttgtcgatc cggtttcagc gtggaaatgt gtcaaagtgc
     6241 gcgaccgtct aaatcagaac cacactctga agccaaaccg ccttgaagcg gaaaccctga
     6301 gtgggattgc gctgtcaggg cgtgacgatg tggcaaaagt tgctgcctgg ttccatcaac
     6361 atggcctgaa ccgactggta ttgagcatgg gcggcgacgg cgtttattac agcgatatca
     6421 gcggtgaaaa tggctggtct gcgccgatca aaaccaatgt tattaatgtt accggagcgg
     6481 gcgatgccat gatggcggga cttgcttcgt gttgggtaga cggaatgccg tttgccgaat
     6541 ctgttcgttt cgcacaggga tgttcgtcaa tggcgctctc ctgtgaatac accaataacc
     6601 ccgatttatc gattgccaac gttatatcgt tagtggagaa cgcagaatgt ctgaattaaa
     6661 aatttcccct gaattattac aaatttctcc ggaagtgcag gacgctttaa aaaacaaaaa
     6721 accggttgtg gcgctggaat cgaccattat ttctcacggg atgccgttcc cacaaaatgc
     6781 ccagaccgca attgaagtag aagaaactat tcgtaaacag ggcgcagtac ctgccactat
     6841 cgccattatt ggcggcgtga tgaaagtggg tttaagcaaa gaaaaaattg aattactggg
     6901 tcgtgaaggg cataacgtga ccaaagttag tcgtcgcgat ttaccttttg ttgttgccgc
     6961 cggaaaaaat ggcgcaacca ctgtggcttc aacgatgatt attgcggcgc ttgccggaat
     7021 taaagtattt gccaccgggg gaattggtgg tgtgcatcgc ggggcggaac ataccttcga
     7081 tatttctgcc gatttgcaag aactggcaaa tactaatgtc accgttgttt gtgccggggc
     7141 gaaatctatt ctcgatttag gattaaccac tgagtattta gaaaccttcg gtgtgccgtt
     7201 aattggctat cagactaaag cgctgcctgc gtttttctgc cgtaccagct cgtttgacgt
     7261 cagcattcgt ctcgacagcg ccagtgaaat tgcccgtgca atggcggtga aatggcaaag
     7321 cgggctgaac ggtggcctcg tggtagcgaa cccgatcccg gaacagtttg cgatgccgga
     7381 ggaatctatc aatgcagcca tagatcaagc cgtcgccgaa gccgaagagc agggcgttat
     7441 tggtaaagaa agtacaccgt tcctgctggc gcgcgttgct gagctgacca gcggtgacag
     7501 cctgaaatcc aatatccagc tggtgttcaa caacgccatt ctggcgagtg aaattgccaa
     7561 agaataccag cgtctcgcgg gttaatttct gacgggcaga aagccgtcgc ccgtactcat
     7621 caagcatacc aaaacctggc gctgacgccg ggtttactgg catgaaggga aaatcattat
     7681 ggatataatg agaagtgttg tggggatggt ggtgttactg gcaatagcat ttctgttgtc
     7741 agtgaataaa aagagcatca gtttgcgcac ggttggagcc gcactgctgc tgcaaatcgc
     7801 tattggtggc atcatgctct acttcccacc gggaaaatgg gcagtagaac aggcggcatt
     7861 aggcgttcat aaagtgatgt cttacagtga tgccggtagc gccttcattt ttggttcgct
     7921 ggttgggccg aaaatggatg tcctgtttga cggtgcgggt tttatcttcg cctttcgcgt
     7981 acttccggcg attattttcg ttactgcgct catcagtctg ctgtactaca ttggcgtgat
     8041 ggggctgctg attcgcatcc ttggcagcat tttccagaaa gccctcaaca tcagcaaaat
     8101 cgaatctttt gttgcggtta ccactatttt cctcgggcaa aatgagatcc cggcgatcgt
     8161 taaaccgttt atcgatcgcg tgaatcgcaa cgagttgttt accgcaattt gtagcgggat
     8221 ggcgtccatt gctggttcgg tgatgattgg ttatgccgga atgggcgtac caattgacta
     8281 cctgttagcg gcatcgctga tggcgatccc cggcggtatt ttgtttgcac gtattcttag
     8341 cccggcaacc gagccttcgc aggtcacatt tgaaaatctg tcgttcagcg aaacgccgcc
     8401 aaaaagcttt atcgaagcgg cggcgagcgg tgcgatgacc gggctgaaaa tcgccgctgg
     8461 tgtagcgacg gtggtaatgg cgtttgtcgc aattattgcg ctgatcaacg gcattatcgg
     8521 cggaattggc ggctggtttg gtttcgccaa tgcctctctg gaaagtattt ttggctatgt
     8581 gctggcaccg ctggcgtgga tcatgggtgt ggactggagt gatgccaacc ttgctggtag
     8641 cctgattggg cagaaactgg cgattaacga attcgtcgct tacctgagtt tctccccata
     8701 cctgcaaacg ggcggcacgc tggaagtgaa aaccgtggcg attatatcct ttgcgctttg
     8761 tggttttgct aactttggtt ctatcggtgt tgtcgttggc gcattttcgg ctatttcgcc
     8821 aaaacgtgct ccagaaatcg cccagcttgg tttacgggcg ctggcagcag caacgctttc
     8881 caacctgatg agtgcgacta ttgccgggtt ctttattggt ctggcgtaat gctcgatttg
     8941 tagaccggat aaaacgtatt aagcgttgca tccggcaatc gcgctattgc aaataatttt
     9001 tactgcatca tcccggagaa tttattctcc gggtccatct ccagcgccag tcctgacaac
     9061 tgctttctgt ttttaattag atatcctttc ttttaattaa taaaccgtcg tgaatgaatt
     9121 gcgcgagaac atataaaaga tgtcgataag aaacgcctaa atactctgcc gcttgcgtat
     9181 gcttttcgtg ataaagatca ccttcttgtg agagtaatat aaatgctgcc aggcgattca
     9241 ctaacggaaa tgattgattc tgagttaaag aaacaatatt acgataattt ttatgactta
     9301 aggtgacgca aagttttcgt aaaaataggg tgtcgtttaa taacagcgga cggtaatgtt
     9361 tcatagggag cgcaaggcac caacactctt caatagcctg caccgcacgc ggttcatggt
     9421 ctttgtcgat taactcaatc tcgccaataa aacagggagc ggcaaagaaa tcgatcagcg
     9481 acacgcgacc attggcaagc gtggcgtaaa gcctggcgcg gcctcgcgtc agataaaaca
     9541 gccatgacgg ctgttgccct tcctgcacaa tgtagtcacg cgctaaaaaa tgaaacagcc
     9601 gcgtatcggc tgaaacatcc gttgaaaagc aatccttaaa cgcagattca ctcatcaatt
     9661 gctgcttaag gtcattattg tggatttctt tcacgatgtt tatccgctat gagatttctc
     9721 atatttagca tacgtacgct gttgttaaga ttcaatcatt ggcccggtcg aattttcttt
     9781 gctgtagaaa aaaataacgc aactggaaac agaggaaata aacaatggaa aagagaaaaa
     9841 ttattctgga ttgtgagccg ggtcatgatg atgctattgc aatgatgatg gcagcgaaac
     9901 atccggcaat agatttatta ggcatcacta ttgtagcggg taatcagacg cttgataaaa
     9961 cattaattaa tggcctgaat gtttgccaga aactggagat taatgttccg gtttatgcgg
    10021 ggatgccgca acccattatg cgtaaacaaa tcgttgccga taatattcac ggtgaaaccg
    10081 gactggatgg cccggtattc gagccgctga cccgccaggc agaaagtacc catgcggtga
    10141 aatatatcat cgataccctg atggcaagcg atggcgatat cactctggtg ccggttggtc
    10201 cgctttcaaa tatcgcggtg gcaatgcgta tgcaacccgc aatcctgccc aaaatccgtg
    10261 aaattgtgct aatgggcggc gcttacggta caggcaactt caccccatct gccgagttca
    10321 atatctttgc cgacccggaa gccgcccgcg tggtgttcac ctccggcgtt ccattagtga
    10381 tgatgggcct cgatcttacc aaccagaccg tttgcacccc ggatgtaatt gctcggatgg
    10441 aaagggtagg cggccctgcc ggagagctgt tcagcgacat catgaacttc actctcaaaa
    10501 cgcagttcga atactacggc ctggctggcg gcccggtgca tgacgccacc tgcattggtt
    10561 atctgatcaa ccctgatggc attaaaaccc aggatatgta cgtcgaagtg gacgtcaaca
    10621 gcggcccttg ctacgggcgt acggtctgcg acgaactggg cgttcttggc aagcccgcta
    10681 ataccaaagt cggcatcact attgataccg actggttctg gggcttagtc gaagagtgcg
    10741 tgcgcggcta catcaaaacc cattaagtgt tgataacaag ccgggacagt atcgcaagat
    10801 gcaggcccgg ctatttctga tgggcagaaa gccgtcgccc aaaatgacat gaagagataa
    10861 tcactatgga tgtcatgaga agtgttctgg gaatggtggt actgctggca attgcgtttt
    10921 tactgtcagt aaacaagaag aagatcagcc tgcgtaccgt tggcgcggcg ttagtgttac
    10981 aggtcgtgat tggcgggatt atgctttggt taccgccagg gcgttgggtc gcagaaaaag
    11041 tcgcttttgg cgtccacaaa gtgatggcgt acagcgacgc aggtagcgca tttatcttcg
    11101 gttctctggt cggaccgaaa atggatacct tatttgatgg cgcaggattt atctttggtt
    11161 tcagggtatt accggcaatt atcttcgtca ctgcactggt gagtattctc tactacatcg
    11221 gtgtgatggg gatcttaatt cgcattctcg gcggtatctt ccagaaagct ttaaatatca
    11281 gcaagatcga gtcattcgtc gcggtcacca ccattttcct cgggcaaaac gaaattccgg
    11341 cgatcgtcaa accctttatc gatcgtctga atcgcaatga attatttaca gcgatttgta
    11401 gtggcatggc ctcgattgct ggttcgacaa tgattggtta cgccgcactg ggcgtacctg
    11461 tggaatattt gctggcggca tcgttaatgg cgatccctgg cgggatcttg tttgcccgcc
    11521 tgttaagccc ggctacggaa tcttcgcagg tttcttttaa taacctctct ttcaccgaaa
    11581 caccgccaaa aagcattatt gaagccgatg cgacaggggc aatgaccggg ctgaaaatcg
    11641 ccgccggtgt agcgacagtt gttatggcat ttgtcgccat cattgcgtta attaacggta
    11701 ttatcggcgg cgttggcggc tggtttggtt ttgaacatgc ctcgctggag tccattttag
    11761 gttacctgct ggccccactt gcgtgggtaa tgggtgttga ctggagtgat gcaaatcttg
    11821 ccgggagttt gattggacag aaactggcaa taaatgaatt tgtcgcttat ctcaatttct
    11881 caccctatct gcaaacggct ggcactctgg atgctaaaac cgtggcgatt atttccttcg
    11941 cgttgtgcgg tttcgctaac tttggttcta tcggggtggt ggtgggggcg ttttctgcgg
    12001 ttgcgccaca ccgtgcgccg gaaatcgccc agcttggttt acgcgcgctg gcggcggcga
    12061 cactttctaa tctgatgagt gccaccattg ccgggttctt tattggttta gcgtagtgtg
    12121 caatttatga gccggattag atgcggtcaa gcgtcgaatc cggcaacatt gttacaccat
    12181 tggcactaat gacagcgcgt tatcggcaga cagggtaggg ttgtttaagc tgccgctggc
    12241 gcgtgaaatt gccgcgcagg ccatcgcaaa acgggcgctg tcacggaaat tgtttccctc
    12301 cagaaagcta tataccagac ccgccataaa accatcgtca gcaccaaaac tgtctaccgt
    12361 cgtatgcgct ggcacagtca gcaaaaattg ttcaccatcc ttttcgctgc aataaactga
    12421 ctcatcgggc aaataaacaa acagttgctg aacaccttgc tgatgcaatg cattcactgc
    12481 ggcattacgg tcagcatcgc tggtgatcgc ctgtccccat aaaatttcca gctccggtaa
    12541 agtgggtttc agggtgtgaa tatgcgccag ccagtgtttg attttgcccg ctttgaattc
    12601 tgaaacggta tcgacaaaca ccgggatctc atcagcgagg gtaaagaccc attccagcgc
    12661 ctcggctgtc aggttgcaat cagccagtac cacgcccgca tgacgaagta aatcgcgcga
    12721 cccgttcagt agttgcggtg tcaactgttc cagcagatgg gtatcgttaa ttgccagcac
    12781 ggtttgatcg tctcgattgg caattgccag atacgtcgat gtgctttgac catgcaaacg
    12841 aacgcagccg gagacattca cgccggcacg gcgcgtttct tccaggagca tttcgccata
    12901 aaagtcatcg ccaatcactg aaagcaaatg gacgtcacgg cctaacagcg ccagattgtg
    12961 ggcgatattg cgtccaacgc cgcctgccga acaatgaatt gtaccgggat gagaagccgc
    13021 ttgcgggtaa tggatatccg ccatcccgcg aatatccata ttgattgtcc ccaccactac
    13081 gcagtattcc tgctcggtga gaatgtaacc tttgtcttta atccggcctt tgcgcattaa
    13141 atccataata tgcgccgcaa cgcgcgaacg actgatttgc agcatgtccg caatttcgtt
    13201 ctgctgaatc agcgggttac gccgtaaaat tgcaaggatc tccttttccc ggttatttat
    13261 cttcaggcta ccgctttttc agtttgttgc gctttcagcc aggcgatctc ttctgcccag
    13321 atatccgggt tgatggtttc aaggatcagc ggaatgccgt cgaaacggtc gttctgcata
    13381 atccagcgga acgcatcatg accgatattg ccttcaccga ggctatgatg gcggtcaacg
    13441 cggctgccaa aggtactttt cgcatcgtta aggtgcatcc cgcgcaaata cttaaagccg
    13501 acaatacggg caaaatcagc gaacgttttt tcgcattcgg ctggagtacg caaatcatac
    13561 ccggcagcga aagcatggca ggtatcaatg cagacgccga cgcgggattt atcttccacg
    13621 ccgtcgataa tcgccgcgag atgttcgaat ttaaacccta agttactgcc ctgaccggcg
    13681 gtgttttcaa tcaccgctgt tacaccttga gttttatcca gcgcaatgtt gatggattcg
    13741 gcaatacgcg caaggcaatc ctcttctgaa atctgcatca gatggctgcc agggtggaag
    13801 ttgagcaaag aaagccccag ttgttcgcaa cgctgcattt catctataaa ggcatcgcgc
    13861 gatttttcca gagcttcagt gaccggatgt ccgaggttga tcagataact gtcgtgggga
    13921 agaatttgcg ccgatgtgta gtgatatttt tcacaggcgg ctttgaattc atcgatggtt
    13981 tgcgtcgtga gcggtgcggc acgccactga cgttggtttt tggtgaacaa ggcaaacgcg
    14041 gttgcgtcga tttcggcggc gcgaattgcg gcatttgcca gaccgccagc agcactaacg
    14101 tgcgctccaa tgtatttcat gcgaggactc ctgttaaacc cgctggagga aaacggtaat
    14161 gatagcgggt taacaggagc aagaagtagt ggtttatgcg atgacgcttt gaatcacata
    14221 gttaatcgca ccaccaccaa caatcagcca ggcaaacagt accagtgcca tcagcagagg
    14281 tttcgcccca gcttttttca gcgcgctgac gtgagtggtc agacccagcg ccgccatcgc
    14341 cattgccagc aggaaggtat ccagcgttac cagcatgttc accacgctct gcggtaacag
    14401 gtggaacgag ttaaagatgg caactacgat gaacaagatg gcaaaccacg gaatagtgat
    14461 tttgcttttc tcgccgctgt tcgccccaga cagctgttta acacgcgccg ccagcaggat
    14521 gaggaacgga gccagcatca tcacgcgcag cattttggaa ataactgctg cgttttctgc
    14581 atccgggctg atggcatgac ctgccgccac cacctgcgcc acttcgtgca cagtagaacc
    14641 aatgtagata ccgaaagttt ccggactaaa ccattgagac atcagcggat atatcgccgg
    14701 gtagaggaaa atcgcgacgg tcccgaagat aacaacggtt gcaacagcca cggttacttt
    14761 actggcttcc gctttcacta ccggctcagt cgccagtacc gcggcagcac cacagatact
    14821 gctaccggca ccgatcaacc aactggtgtg cttatccaga ccaaacactt tctgccccag
    14881 gaagcaagcc agcaggaagg tactggacag cgtcagcacg tcaatgatga tcccactgat
    14941 accgacatcg gcaatttgcg agaacgtcag acggaagcca taaagaatga tacccagacg
    15001 taataaatat tgcttggcaa acagcacacc accgtcacag cttttccaga tgtgcggata
    15061 gatggtgttg cctaaaacca tccccaacaa gattgcgagg gtgagggcac taaacccggc
    15121 acccgcaacc gcgggaatgg aaccacccca cagggcgacc ccggtgataa ctgcactcag
    15181 ggctaacccc ggaataaaat gccacagtgt acgatgttgt ttctgtaagg tgatattcgt
    15241 cataaccctc tcctttaacc gggcataagg ttacgactaa ctggtttaaa aataaaattg
    15301 attatatatt tataattaat ctttataagt ggtaagcgac tatgcacatc accctccggc
    15361 agttggaagt ttttgcagaa gtattgaaaa gtggatcaac cacccaggcg tcggtgatgc
    15421 tggcgttgtc gcaatcagca gtgagcgcag ccttgactga cctggaaggg cagcttggcg
    15481 ttcaactgtt tgatcgcgtg gggaaaagac tggttgttaa tgaacacggg cggctgctct
    15541 atccgcgagc gttggcactg cttgaacagg cggttgaaat cgaacaactg tttcgcgaag
    15601 acaacggcgc gattcgtatc tatgccagta gtaccatcgg taactacatt ctgcctgcag
    15661 ttatcgcccg ttatcgccat gattatccgc agttgccgat tgaacttagc gttgggaaca
    15721 gccaggacgt gatgcaagcg gtgctggatt tccgcgttga tattggcttt attgaaggac
    15781 cgtgccacag cactgaaatc atttctgaac cgtggctgga agacgagctg gtggttttcg
    15841 ccgcgccgac ttcgccgttg gctcgtggtc cggtcacctt agaacagctg gccgctgcgc
    15901 cgtggatcct gcgtgaacgc ggttccggca cgcgggatat tgtcgattat ctgttgctgt
    15961 cacatttacc gaagtttgag atggcgatgg aattaggtaa ctccgaggca atcaaacatg
    16021 cggtgcgtca tgggttggga attagttgcc tgtcgcgacg tgtgattgaa gatcaattgc
    16081 aggcaggcac attaagtgaa gttgcggtcc ctctgccgcg cctgatgcgt acgttgtggc
    16141 gtatacatca tcggcaaaaa cacctttcca acgcgctacg gcgctttctg gactattgcg
    16201 atcccgcaaa tgtgccgcgt taagttgctg tacaagaaca tgctggtgct gtgtcgattt
    16261 cgtgacgcag cgccttcagc atgcattcgc cagaaaagag attggctgct ttacttataa
    16321 tccctgggcg atcatgaagg tgtcttataa ccgtgtattt ctgccggaag gattgccaat
    16381 cgtctgctac aatcgcgcca tcatttttaa gatggatagc atttttgtat gggttccgaa
    16441 actaaaacta cagaagcgcc gggcttacgc cgtgaattaa aggcgcgtca cctgacgatg
    16501 attgccattg gcggttccat cggtacaggt ctttttgttg cctctggcgc aacgatttct
    16561 caggcaggtc cgggcggggc attgctctcg tatatgctga ttggcctgat ggtttacttc
    16621 ctgatgacca gtctcggtga attggctgca tatatgccgg tttccggttc gtttgccact
    16681 tacggtcaga actatgttga agaaggcttt ggcttcgcgc tgggctggaa ctactggtac
    16741 aactgggcgg tgactattgc cgttgacctg gttgcagcac agctggtcat gagctggtgg
    16801 ttcccggata caccgggctg gatctggagt gcgttgttcc tcggcgttat cttcctgttg
    16861 aactacatct ctgttcgcgg ctttggtgaa gcggaatact ggttctcact gatcaaagtc
    16921 acgacagtta ttgtctttat catcgttggt gtgctgatga ttatcggtat cttcaaaggc
    16981 gcgcagcctg cgggctggag caactggaca atcggcgaag cgccgtttgc tggtggtttt
    17041 gcggcgatga tcggcgtagc tatgattgtc ggcttctctt tccagggaac cgagctgatc
    17101 ggtattgctg caggcgagtc cgaagatccg gcgaaaaaca ttccacgcgc ggtacgtcag
    17161 gtattctggc gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag cctgattatt
    17221 ccgtataccg atccgagcct gctgcgtaac gatgttaaag acatcagcgt cagtccgttc
    17281 accctggtgt tccagcacgc gggtctgctc tctgcggcgg cggtgatgaa cgcagttatt
    17341 ctgacggcgg tgctgtcagc aggtaactcc ggtatgtatg cgtctactcg tatgctgtac
    17401 accctggcgt gtgacggtaa agcgccgcgc attttcgcta aattgtcgcg tggtggcgtg
    17461 ccgcgtaatg cgctgtatgc gacgacggtg attgccggtt tgtgcttcct gacctccatg
    17521 tttggcaacc agacggtata cctgtggctg ctgaacacct ccgggatgac gggttttatc
    17581 gcctggctgg ggattgccat tagccactat cgcttccgtc gcggttacgt attgcaggga
    17641 cacgacatta acgatctgcc gtaccgttca ggtttcttcc cactggggcc gatcttcgca
    17701 ttcattctgt gtctgattat cactttgggc cagaactacg aagcgttcct gaaagatacc
    17761 attgactggg gcggcgtagc ggcaacgtat attggtatcc cgctgttcct gattatttgg
    17821 ttcggctaca agctgattaa aggaactcac ttcgtacgct acagcgaaat gaagttcccg
    17881 cagaacgata agaaataagt ttcctccctt ccttgctaag ccctctcaac cgagagggct
    17941 ttttcaattc catttccctg acaaatcatg cggatataaa atttaacatt tggattgata
    18001 attgttatcg tttgcattat cgttacgacg caatcaaaaa aggctgacaa atcagaggct
    18061 gttccggctt tctgggatga tcacctgcat acaaataagt ccaccgcgat gctgccgtac
    18121 gcaaggggac gtaaagaaga tgtgagcgat acccatttta ttttcgtagt tacctcatgg
    18181 agatatggaa tgtttaggtt gaaccctttc gtacgggtcg ggctgtgttt gtccgctatt
    18241 tcttgtgcat ggcctgtgtt agcggtcgat gatgatggcg aaacgatggt tgtcactgca
    18301 tcttccgtgg aacaaaatct taaagatgca cccgccagta tcagcgtcat tacccaggaa
    18361 gacctgcagc gaaaaccggt acagaatctg aaggatgtcc tcaaagaagt gcctggcgta
    18421 caactgacga acgaagggga taaccgtaag ggcgttagta ttcgtggtct ggacagcagc
    18481 tacaccctga ttcttgtcga cggtaaacgc gttaactccc gcaatgccgt cttccgccac
    18541 aatgatttcg atctgaactg gatcccggtc gattccatcg aacgtattga agtggttcgt
    18601 ggcccgatgt cgtcgctata cggctccgat gcgctcggcg gtgtagtgaa tatcatcacc
    18661 aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg ataccaccat tcaggaacat
    18721 cgcgatcgcg gtgataccta taacggtcag ttctttacca gtggaccatt aattgatggt
    18781 gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg aaaaggatga cccgcaaaac
    18841 tcaacgacca ccgataccgg agaaacgccg cgtattgaag gattctccag ccgcgacggc
    18901 aatgtcgaat ttgcctagac accgaatcaa aatcacgatt ttactgccgg atacggtttc
    18961 gaccgtcagg atcgtgattc cgattcgctg gacaaaaacc gcctggaacg ccagaactac
    19021 tgcgtcagcc ataatgggcg ttgggattac ggcaccagcg aactgaaatc ctacggtgag
    19081 aaagtcgaga acaaaaaccc tggcaacagc agcccgataa cttccgaaag caatacggtc
    19141 gacggcaaat acacgttgcc gctgacggcg attaatcagt ttctcacggt tggcggtgaa
    19201 tggcgtcacg acaaacttag cgatgcggtg aacctgaccg ggggaaccag ctccaaaacg
    19261 tctgccagcc agtacgcgct gtttgtggaa gatgaatggc ggatcttcga gccgctggcg
    19321 ctgacgaccg gcgtgcgtat ggacgatcac gaaacctacg gtgaacactg gagtccgcgt
    19381 gcctacatgg tttataacgc caccgacacc gtaacggtga aagggggctg ggcgacggca
    19441 tttaaagcac cttctctgtt gcaacttagc cctgactgga cgagcaattc ctgccgtggc
    19501 gcatgtaaga ttgtgggtag cccggatctg aaaccagaaa ccagcgaaag ttgggagctg
    19561 gggctttact acatgggtga agaaggctgg ctggaagggg gtgaatccag cgttatcgtt
    19621 ttccgtaacg atgtgaaaga tcgtatcagc atcagccgta cgtctgacgt caacgctgca
    19681 ccgggctacc aaaactttgt tggttttgag acgggcgcta acggacggcg cataccggta
    19741 tttagctact acagcgttaa caaagctcgt attcagggcg tggaaaccga actgaaaatt
    19801 ccgttcaacg atgaatggaa actgtcgatc aactacacct acaacgatgg tcgtgatgtc
    19861 agcaacggcg aaaacaaacc gctatccgat ctgccgttcc atactgctaa cggtacgctg
    19921 gactggaaac cgctggcgct ggaagactgg tcattctatg tttctggtca ctataccggg
    19981 cagaaacgcg ccgacagcgc gacggctaaa acaccgggcg gttataccat ctggaatacc
    20041 ggcgcggcct ggcaggtgac taaagacgtc aaactgcgcg caggcgtgct gaaccttggc
    20101 gacaaggatc tcagtcgtga cgactacagc tataacgaag acggacgtcg ttactttatg
    20161 gcagtggatt atcgcttctg atgagaagat gcccggcgaa ccgggcggac tttcacttca
    20221 gtaaatactg cgcatggaag cgcaggtgat cctctataaa agaggcgatg aagtagtaac
    20281 tgtgatcata tcccggctga atacgcagcg tcatcggcca cgctttctgg cgcgcggctt
    20341 ctgccaatac cgcaggttgc aactggtcgg caagaaactg atcattatcg ccctgatcga
    20401 taagcgtcgg gatcgcatcc tgcgcgttac tggcatacat cagcgcgcaa ctatcccatt
    20461 ccagcatgca tttttgtcct cacctaaata ggtgctaaac gctttgattc cccacgggac
    20521 gctgcacgga ttcacaatgg gcgcaaaggc cgaaacgctg gtgtatttac ccggattttt
    20581 cagcgccata atcagcgcac cgtgaccacc cattgagtga ccgctaatgg cgcagcggtc
    20641 gctgacatta aattgcgact gaaccagcgc cggtaattca tcgcgcagat aatcatacat
    20701 ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg cgccctggcc
    20761 taaatcgtag ccatcgtcgt tggcaacctt ttcgccgcgc gggctggtgt ctggcatcac
    20821 cagtacaatc cccagttccg ccgctacccg ctgggcaccc gccttggtgg tgaagttctc
    20881 gtcattgcag gttaatccgg aaagccagta cagcactggt ggcggggtgt gatcacgagg
    20941 gggggggaga aagatactga acgtcatcgg gcagtttaag gtactggagt cgtgtcgcca
    21001 gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc atttccatgc gtggctcctt
    21061 gttgtgttgt ttgcaatttc ttcatcatac cgtttattca atgtgctgtg agtaactttc
    21121 acttccgtat ttgcataacg atgttttaac atctgctgat gaaaggcagc ggcaattaca
    21181 ataattatcg ctgtgaatac tggattatgt gcgccgcctc acgcacaata atcaagctgt
    21241 aaatcagctt aataactttg cccccacgca gggtggaggc gtcacacctg caggagaaat
    21301 cataaatgcc atcactcagt aaagaagcgg ccctggttca tgaagcgtta gttgcgagag
    21361 gactggaaac accgctgcgc ccgcccgtgc atgaaatgga taacgaaacg cgcaaaagcc
    21421 ttattgctgg tcatatgacc gaaatcatgc agctgctgaa tctcgacctg gctgatgaca
    21481 gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt cgatgaaatt ttctccggtc
    21541 tggattacgc caatttcccg aaaatcaccc tcattgaaaa caaaatgaag gtcgatgaaa
    21601 tggtcaccgt gcgcgatatc actctgacca gtacctgtga acaccatttt gttaccatcg
    21661 atggcaaagc gacggtggct tatatcccga aagattcggt gatcggtctg tcaaaaatta
    21721 accgtatcgt gcagttcttt gcccagcgtc cgcaggtgca ggaacgtctg acgcagcaaa
    21781 ttcttattgc gctacaaacg ctgctgggca ccaataacgt ggctgtctcg atcgacgcgg
    21841 tgcattactg cgtgaaggcg cgtggcatcc gcgatgcaac cagtgccacg acaacgacct
    21901 ctcttggtgg attgttcaaa tccagtcaga atacgcgcca cgagtttctg cgcgctgtgc
    21961 gccatcacaa ctgattaaaa ggcaggaacc atggagcgca acgtcacgct cgattttgtt
    22021 cgcggcgtcg ccattctggg gatcctgcta ttaaacatca gcgcctttgg gctaccaaag
    22081 gcggcttatc tcaatcccgc ctggtacgat gctattacgc cgcaggatgc atggacctgg
    22141 gcatttctcg atctcatcgg ccaggtgaaa ttcctcacgc tttttgcgct gctgtttggt
    22201 gctggcctgc aaatgttgct gccccgtggc agacgctgga tccagtcgcg gttaacgctg
    22261 ttagtcttgt tgggctttat tcacggttta ttgttctggg acggcgatat tctgctggct
    22321 tacgggctgg tggggcttgg cgttttgctg ttattggggc tgatttccga cagccagacc
    22381 agccgcgcct ggacgccgga tgcatcggct attttgtatg aaaaatactg gaagcttcac
    22441 ggcggcgttg aagcgatcag taatcgtgcc gatggtgttg gcaacagttt actggcactg
    22501 ggcgcacagt atggctggca actggcaggg atgatgctca ttggtgcggc gctgatgcgc
    22561 agcggctggc tgaaagggca gttcagctta cgtcactatc gtcgtactgg ttttgtgctg
    22621 gtggcgattg gggtgaccat taaccttcct gccatcgccc tgcaatggca gctggactgg
    22681 gcgtatcgct ggtgtgcctt cttacttcaa atgccgcggg aactgagtgc gccgtttcag
    22741 gcgattggct atgcgtcgct gttttatggc ttctggccgc aattgagccg ctttaagctg
    22801 gtgcttgcga tcgcctgcgt cggacggatg gcgctgacca actatctatt gcaaacgctg
    22861 atttgtacca cgctttttta ccacctcggt ttgtttatgc attttgaccg cctggagctg
    22921 ctggcgtttg ttattccggt atggctggcg aatatcctct tctctgttat ctggctgcgt
    22981 tacttccgcc aggggccggt ggaatggctc tggcgtcagt taactttgcg tgctgccgga
    23041 ccggcaatat ctaaaacatc aagataacga tctggattac attcgttaac aaaacggctg
    23101 taaccgtttc cattgctgtg actcgattca cgaagtcctg tattcagtgc tgacaaaata
    23161 gccgccagca agcagtcatt tactgcaatc tcataacagg tagtgaatat gatcaccatt
    23221 cgtgatgtag cgcgtcaggc tggcgtctct gtggcaacgg tttcccgggt gctcaataac
    23281 agcacgctgg tcagtgccga cacgcgtgaa gcagtaatga aagccgtgag tgagctggat
    23341 tatcggccaa acgccaatgc ccaggcgctg gcaactcagg ttagcgacac cattggcgtg
    23401 gtggtgatgg acgtttctga tgcgtttttc ggcgcgctgg taaaagcggt ggatctggtc
    23461 gcccagcagc atcagaaata cgtgctaatc ggcaatagct atcatgaagc ggaaaaagag
    23521 cgtcacgcca ttgaggtgtt aattcgccag cgttgtaatg cgttgattgt tcactcaaaa
    23581 gcattgagtg acgatgaact ggcgcaattt atggataaca ttcccggtat ggtgttaatc
    23641 aaccgcgttg tgccggggta cgcccatcgt tgcgtttgcc tggataatct cagcggtgcc
    23701 cgaatggcga cgcgcatgtt gctgaataac ggtcatcaac gtattggtta tctttcttcc
    23761 agccacggca ttgaagatga cgccatgcgt aaagcaggct ggatgagtgc gttgaaagag
    23821 caggatatta ttccgccgga aagctggatt ggcactggta cgccggacat gccgggcggt
    23881 gaggcggcga tggttgaact gctggggcgc aatctacaac ttaccgctgt atttgcttat
    23941 aacgacaata tggccgctgg cgcactgaca gcattaaaag ataatggcat tgcgattccg
    24001 ttacatctct caatcatcgg tttcgatgat attcccatcg cccgttacac cgacccgcaa
    24061 ttaacgaccg tgcgttatcc cattgcttca atggctaaat tagccaccga actggccttg
    24121 cagggggcag caggcaatat tgatcctcgt gccagccact gttttatgcc gacgttagtg
    24181 cgtcgccatt ctgtcgcaac gcgccagaat gcggcggcga tcactaactc aacaaatcag
    24241 gcgatgtaac cgctttcaat ctgtgagtga tttcacagta tcttaacaat gtgatagcta
    24301 tgattgcacc gttttaatgt tgtaacccgt atgtaacagt gaataatcac tttcaccgag
    24361 gtaatagcgt cataacaaca attaaagccg ttttctggag cgttaccggg catggaagaa
    24421 cgaattttaa aaagtgagct tcggcgttca gtaacacttc attaactcta ctgccccgcc
    24481 gagcatttat ctcaagcact accctgcata agaaaaaccg gagataccat gaataagaag
    24541 gtgttaaccc tgtctgctgt gatggccagc atgttattcg gtgccgctgc acacgctgct
    24601 gatactcgca ttggtgtaac aatctataag tacgacgata actttatgtc tgtagtgcgc
    24661 aaggctattg agcaagatgc gaaagccgcg ccagatgttc agctgctgat gaatgattct
    24721 cagaatgacc agtccaagca gaacgatcag atcgacgtat tgctggcgaa aggggtgaag
    24781 gcactggcaa tcaacctggt tgacccggca gctgcgggta cggtgattga gaaagcgcgt
    24841 gggcaaaacg tgccggtggt tttcttcaac aaagaaccgt ctcgtaaggc gctggatagc
    24901 tacgacaaag cctactacgt tggcactgac tccaaagagt ccggcattat tcagggcgat
    24961 ttgattgcta aacactgggc ggcgaatcag ggttgggatc tgaacaaaga cggtcagatt
    25021 cagttcgtac tgctgaaagg tgaaccgggc catccggatg cagaagcacg taccacttac
    25081 gtgattaaag aattgaacga taaaggcatc aaaactgaac agttacagtt agataccgca
    25141 atgtgggaca ccgctcaggc gaaagataag atggacgcct ggctgtctgg cccgaacgcc
    25201 aacaaaatcg aagtggttat cgccaacaac gatgcgatgg caatgggcgc ggtagaagca
    25261 ctgaaagcac acaacaagtc cagcattccg gtgtttggcg tcgatgcgct gccagaagcg
    25321 ctggcgctgg tgaaatccgg tgcactggcg ggcacagtac tgaacgatgc taacaaccag
    25381 gcgaaagcga cctttgatct ggcgaaaaac ctggccgatg gcaaaggtgc ggctgatggc
    25441 accaactgga aaatcgacaa caaagtggtc cgcgtacctt atgttggcgt agataaagac
    25501 aacttggctg aattcagcaa gaaataagac tgattgggcg cagtcgttta ctgcgccctt
    25561 attgtatggc caacaaggta aaattatatg gtcagctcaa cgactccgtc ttccggggaa
    25621 tacttgttgg aaatgagcgg tatcaacaag tcctttcctg gtgttaaggc acttgataac
    25681 gttaatttaa aagtccggcc acattctatc catgcattaa tgggggaaaa cggtgcagga
    25741 aaatcgacat tattaaaatg cctgtttggt atttatcaaa aagactccgg caccatttta
    25801 ttccagggta aagagatcga tttccattcc gccaaagagg cactggaaaa tggtatttcg
    25861 atggtacacc aggagttaaa cctggtatta caacgttcgg tgatggataa catgtggctg
    25921 gggcgatatc ccaccaaagg catgtttgtc gatcaggaca aaatgtaccg cgaaaccaaa
    25981 gcgatttttg atgaactgga tattgatatc gatccgcgtg cgcgcgtcgg cacattatcc
    26041 gtttcgcaaa tgcagatgat cgaaatcgcc aaagcgtttt cctataacgc gaaaattgtg
    26101 attatggatg aaccgacttc ttcgttaacc gaaaaagagg tcaatcatct gttcactatt
    26161 attcgtaaat taaaagagcg cggctgcggt attgtttata tctcgcataa aatggaagaa
    26221 atcttccagt tatgtgatga agttaccgta ttgcgcgacg gtcagtggat cgccaccgaa
    26281 ccgctggcag gactgacgat ggacaagatc atcgccatga tggttgggcg ttctcttaat
    26341 cagcgttttc ctgacaaaga aaacaagccg ggcgaagtca tcctcgaggt gcgtaaccta
    26401 acgtcactgc gccagccgtc gattcgcgat gtctcgtttg atctgcataa aggggagatc
    26461 ctcggtattg ccgggctggt gggggcgaaa cgtaccgata ttgttgagac gttatttggt
    26521 attcgcgaga aatcggctgg caccattacg ttgcacggca aacagatcaa taaccataat
    26581 gccaacgaag ccataaacca cggatttgca ctggtaactg aggagcgccg ctcaacggga
    26641 atttatgcct atctggatat tggttttaac tcgttaattt ccaatattcg caactacaaa
    26701 aataaagttg gtttactgga taactcgcgg atgaaaagcg atacccagtg ggtgattgat
    26761 tcgatgcggg taaaaacgcc gggtcatcgg acgcaaattg gttcgctctc cggtggtaat
    26821 cagcaaaagg tgattattgg tcgctggcta ctaacgcaac cagaaatatt aatgctcgat
    26881 gaaccgacgc gcggtattga tgtcggggcg aagtttgaaa tttatcagtt aattgccgaa
    26941 ctggcgaaga aaggcaaggg gattattatt atctcctctg aaatgcctga gttgttaggg
    27001 ataacagacc gtattctggt catgagcaat ggtctcgttt ccggaattgt cgatacaaaa
    27061 acaacaacgc aaaacgaaat tctgcgtctt gcgtctttgc acctttaaga ttaggggctt
    27121 cccatgagtg cgttaaataa gaaaagtttt cttacttacc tgaaagaggg cggtatttac
    27181 gtcgttcttt tagttttgct ggcgattatt attttccagg acccaacatt tttaagtctg
    27241 ttgaacttaa gtaatattct cacccagtca tcggtgcgta ttattatcgc gctcggtgtg
    27301 gcagggttaa ttgtcaccca ggggaccgat ctttctgctg gtcgtcaggt agggctggcg
    27361 gcagtggtgg ctgcgacatt attgcagtcc atggataacg ccaacaaagt gttcccggaa
    27421 atggcgacga tgccgattgc gctggttatt ctgattgtct gtgccattgg tgcggtgatc
    27481 ggtttgatca acggtctgat tatcgcttat ctcaacgtga cgccgttcat taccacgctc
    27541 ggcacgatga tcatcgtcta tggcatcaac tcgctctatt acgactttgt cggggcgtcg
    27601 ccaatttctg gttttgacag tggcttctct acctttgctc agggctttgt cgcgctgggg
    27661 agtttccgtc tctcttacat caccttctac gcgttgattg cggtggcgtt cgtctgggtg
    27721 ttgtggaaca aaacccgctt cggtaagaac atttttgcca ttggcggtaa cccggaagcg
    27781 gcaaaaatat ctggtgtcaa cgtcggcctg aacctgctga tgatctacgc gttgtctggc
    27841 gtgttctatg cctttggcgg gatgttagaa gccggacgta tcggctctgc caccaacaac
    27901 ctcggcttta tgtatgagct ggatgctatc gcggcgtgcg tggtaggcgg tgtatcgttc
    27961 agcggcggtg tggggacggt gattggcgtg gtgaccgggg taattatttt taccgtcatc
    28021 aactatggcc tgacgtatat cggcgtaaac ccatactggc agtacatcat caaaggggcg
    28081 attattatct tcgccgtagc gctggattca ctgaaatacg cgcgtaagaa gtgatgtgat
    28141 tgcggatatt gccggatgcg tctctggttt tacaacgtta ccgggtgttc tttctcgcct
    28201 ttcttaaact tcacttcccc gagctcaata caacccaccg ggcagacgtg accacaaagc
    28261 agacaaccca cacatttctc ggtattacaa tgcggtgtgc gggttttctc gctccattcc
    28321 atcgcctggt gaccgccgtc gtaacaggaa atataacagc gtccacagcc aacacatttg
    28381 tcaagattga tacggggata gacaatataa ctgcggtcta aatcttccgc cgggacaata
    28441 ttgttattcg ccagacctac catctcctgc aacgaatcaa atccctgatc ggcgagataa
    28501 tgcgataacc cgctcgccat atcttctacg atgcgatagc cgtactgcat aatgccggtg
    28561 gtgacctgta acgttgctgc gccgagcaat aaaaactccg ccgcatcttc ccaggtttca
    28621 atgccgccga taccgctgat tgggaaatcg cgcagttctg gatgggtgcg catttgctga
    28681 atgaagcgca gggcgatcgg ttttaccgct ttaccggaat atccggaaat actcgattta
    28741 ccgttaacga tcggcatacc gattttctga ttaagatcga tattggtgat ggatttaacg
    28801 gtgttaattg ccgcaatgcc atctgcgccg ccgcgcttcg ccgccagcgc cacttcgcac
    28861 atatcaccga tattcggcgt catcttcgcc agcattggca gcgtggaacc ccgtttcact
    28921 gcccgacaat atttttctac cagctccggg ctttgcccga catcgctacc catcgcatga
    28981 gaagtcattt gcggacagga gaagttacac tcgatcatat ccgcgccagc tgctcccatt
    29041 gctgctcatt ttcccccatg atcgaagcga tcaatacttt gtccgggtaa tcttccttca
    29101 gccgacgcag ggcggtcaga ttctcttcca acggatgttc agcaatctgc tccatatttt
    29161 tgaagccgat aaaaccggta tcttctttca ccagatgatc aaaacgcggc gagacttcgt
    29221 tggcgataaa aaagccgatc gttttaaaca ccacaccgcc ccaacctgtg tcgtaggctt
    29281 tggcacacat ctcatagcag ttgcctaccg gcgaagaaga gaggcagaac gggttgggaa
    29341 agttcacgcc gcaaaaagta atcgaaagat ctttcgttaa catgagcaag ctccctctaa
    29401 atagtgatga atcgcctcgg cggcttcttt cccggttttc acggcataga ccacggtttt
    29461 gtcaccctca acaatatcgc cagcagcaaa gacttgcgga tcgcggatct gataattttg
    29521 tgttttgatg gtgttacgct gctgctctaa ctccgcaaag gcatccagtc tggcatgctg
    29581 accgacggcg agaataattt tatctgccgc catcgtcagt tcgcctgata accgtacatg
    29641 cttaaacgtg actttattgc cttccacggc tactggcgtg aatccatcga tgatcgaaac
    29701 gcccagctcc cgggcgctgg caaactcttt ttcgcttgcc ggaaactcat ctaactcttc
    29761 acgtgctacg caagttaccg cctgacagcc gagaactttc agcgtgctgg ctacgtccat
    29821 cgcgacatca ccgccgccga taattaatgc gctttgtgga atgctgatat cgccttgcgc
    29881 ctgtcgtaca cgttgcagga agtcgacggc aatctcaacg tcgctatgct caaacagcgg
    29941 tagcccggaa ccgcttgata accccacggt gaccagtacc gcgcggtttt ccgctttcag
    30001 ttgctccagg gtgagtgttt taccgatttc gttattgcac ttaatggtca cgcccatttt
    30061 ttcaatacgg gcgatctctg catccagcac tgattgcggt aaccggaatt gcggaatacc
    30121 gttacgcaac cagccaccgg ggtgcgcttc tttctcataa atcgtgacgt cataaccctg
    30181 gtttgtcagt gtcacactgg cctgcaatcc ggcaggacct gcgccaataa tcgcgacttt
    30241 gccgagcgtt ttagtaccgg gctgataaat ttccatcccg gtttgttgtt caaaatcagt
    30301 aacaaaacgc tgtaagcggc cgatatcaat gggagcatca acaccggcac gggtacaacc
    30361 gctttggcat aatttctccg tcgggcacac tctggcacaa acggcaccga gggcattatt
    30421 ttcgcgaatt gtctcggcag cgcctttaaa attacgaaag tagattgagc gaataaattt
    30481 ccccggatcg gtctgtgccg ggcaagcctg actacaggga gcgtcgtgac ataataaaca
    30541 gcgagaggct tctttaatcg ccagtaacgg cgtaaaagcc ggagtgagtt catccagata
    30601 attttgttgc ggcatgtgat cttccttaat gctatgagta attatggcga acgctaaata
    30661 aatattgcgt gctgatatta aggctatgcg cgtggggaaa tttaagctgt gaaaggattc
    30721 acaaatagct gcggttcaga atgttatatc cgcagcttat tgagtgaagt aataattatt
    30781 ctcaataatt aacagaaaag agaa
//
LOCUS       DAZPAB010000026        30453 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZPAB010000026 DAZPAB010000000
VERSION     DAZPAB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30453
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.026"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..459
                     /locus_tag="ACHUPG_001077"
     CDS             <1..459
                     /locus_tag="ACHUPG_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFR0860779.1"
                     /translation="VEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREE
                     IERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIEL
                     PEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            689..1072
                     /gene="secE"
                     /locus_tag="ACHUPG_001078"
     CDS             689..1072
                     /gene="secE"
                     /locus_tag="ACHUPG_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR0860780.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1074..1619
                     /gene="nusG"
                     /locus_tag="ACHUPG_001079"
     CDS             1074..1619
                     /gene="nusG"
                     /locus_tag="ACHUPG_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR0860781.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1778..2206
                     /gene="rplK"
                     /locus_tag="ACHUPG_001080"
     CDS             1778..2206
                     /gene="rplK"
                     /locus_tag="ACHUPG_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR0860782.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2210..2914
                     /gene="rplA"
                     /locus_tag="ACHUPG_001081"
     CDS             2210..2914
                     /gene="rplA"
                     /locus_tag="ACHUPG_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR0860783.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3327..3824
                     /gene="rplJ"
                     /locus_tag="ACHUPG_001082"
     CDS             3327..3824
                     /gene="rplJ"
                     /locus_tag="ACHUPG_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR0860784.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3891..4256
                     /gene="rplL"
                     /locus_tag="ACHUPG_001083"
     CDS             3891..4256
                     /gene="rplL"
                     /locus_tag="ACHUPG_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR0860785.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4576..8604
                     /gene="rpoB"
                     /locus_tag="ACHUPG_001084"
     CDS             4576..8604
                     /gene="rpoB"
                     /locus_tag="ACHUPG_001084"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR0860786.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8681..12904
                     /gene="rpoC"
                     /locus_tag="ACHUPG_001085"
     CDS             8681..12904
                     /gene="rpoC"
                     /locus_tag="ACHUPG_001085"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR0860787.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13146..14279)
                     /gene="thiH"
                     /locus_tag="ACHUPG_001086"
     CDS             complement(13146..14279)
                     /gene="thiH"
                     /locus_tag="ACHUPG_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR0860788.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14276..15046)
                     /gene="thiG"
                     /locus_tag="ACHUPG_001087"
     CDS             complement(14276..15046)
                     /gene="thiG"
                     /locus_tag="ACHUPG_001087"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR0860789.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(15048..15248)
                     /gene="thiS"
                     /locus_tag="ACHUPG_001088"
     CDS             complement(15048..15248)
                     /gene="thiS"
                     /locus_tag="ACHUPG_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR0860790.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15232..15987)
                     /gene="thiF"
                     /locus_tag="ACHUPG_001089"
     CDS             complement(15232..15987)
                     /gene="thiF"
                     /locus_tag="ACHUPG_001089"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR0860791.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15980..16615)
                     /gene="thiE"
                     /locus_tag="ACHUPG_001090"
     CDS             complement(15980..16615)
                     /gene="thiE"
                     /locus_tag="ACHUPG_001090"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR0860792.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16615..18510)
                     /gene="thiC"
                     /locus_tag="ACHUPG_001091"
     CDS             complement(16615..18510)
                     /gene="thiC"
                     /locus_tag="ACHUPG_001091"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR0860793.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18591..18691)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18744..19220)
                     /gene="rsd"
                     /locus_tag="ACHUPG_001092"
     CDS             complement(18744..19220)
                     /gene="rsd"
                     /locus_tag="ACHUPG_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFR0860794.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19315..20088
                     /gene="nudC"
                     /locus_tag="ACHUPG_001093"
     CDS             19315..20088
                     /gene="nudC"
                     /locus_tag="ACHUPG_001093"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR0860795.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20128..21192
                     /gene="hemE"
                     /locus_tag="ACHUPG_001094"
     CDS             20128..21192
                     /gene="hemE"
                     /locus_tag="ACHUPG_001094"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR0860796.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21349..22020
                     /gene="nfi"
                     /locus_tag="ACHUPG_001095"
     CDS             21349..22020
                     /gene="nfi"
                     /locus_tag="ACHUPG_001095"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR0860797.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22063..22653
                     /locus_tag="ACHUPG_001096"
     CDS             22063..22653
                     /locus_tag="ACHUPG_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR0860798.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22840..23112
                     /gene="hupA"
                     /locus_tag="ACHUPG_001097"
     CDS             22840..23112
                     /gene="hupA"
                     /locus_tag="ACHUPG_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR0860799.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23125..23820
                     /locus_tag="ACHUPG_001098"
     CDS             23125..23820
                     /locus_tag="ACHUPG_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR0860800.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23822..24247)
                     /gene="zraP"
                     /locus_tag="ACHUPG_001099"
     CDS             complement(23822..24247)
                     /gene="zraP"
                     /locus_tag="ACHUPG_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR0860801.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24485..25861
                     /gene="zraS"
                     /locus_tag="ACHUPG_001100"
     CDS             24485..25861
                     /gene="zraS"
                     /locus_tag="ACHUPG_001100"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR0860802.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25858..27183
                     /gene="zraR"
                     /locus_tag="ACHUPG_001101"
     CDS             25858..27183
                     /gene="zraR"
                     /locus_tag="ACHUPG_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR0860803.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27180..28469)
                     /gene="purD"
                     /locus_tag="ACHUPG_001102"
     CDS             complement(27180..28469)
                     /gene="purD"
                     /locus_tag="ACHUPG_001102"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR0860804.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28481..30070)
                     /gene="purH"
                     /locus_tag="ACHUPG_001103"
     CDS             complement(28481..30070)
                     /gene="purH"
                     /locus_tag="ACHUPG_001103"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR0860805.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7134 a         7929 c         8382 g         7008 t
ORIGIN      
        1 gttgaaatcg ttggtatcaa agagactcag aagtctacct gtactggcgt tgaaatgttc
       61 cgcaaactgc tggacgaagg gcgtgctggt gagaacgtag gtgttctgct gcgtggtatc
      121 aaacgtgaag aaatcgaacg tggtcaggta ctggctaagc cgggcaccat caagccgcac
      181 accaagttcg aatctgaagt gtacattctg tccaaagatg aaggcggtcg tcatactccg
      241 ttcttcaaag gctaccgtcc gcagttctac ttccgtacta ctgacgtgac tggtaccatc
      301 gaactgccgg aaggcgtaga gatggtaatg ccgggcgaca acatcaaaat ggttgttacc
      361 ctgatccacc cgatcgcgat ggacgacggt ctgcgtttcg caatccgtga aggcggccgt
      421 accgttggcg cgggcgttgt agcaaaagtt ctgagctaat tgccgataac atttgacgca
      481 atgcgcacta aaagggcatc atttgatgcc ctttttgcac gctttcgtac cagaacctgg
      541 ctcatcagtg attttctttg tcataatcat tgctgagaca ggctctgttg agggcgtata
      601 atccgaaaag ctaatacgcg tttcgatttg gtttgcctcg cgatcgcggg gtgaaaatgt
      661 ttgtagaaaa cttctgacag gttggtttat gagtgcgaat accgaagctc aaggaagcgg
      721 gcgcggcctg gaagcgatga agtgggtcgt tgtggtggca ttgctcctgg cggcgattgt
      781 cggcaactac ctttatcgcg acattatgct gccgctgcgt gcgctggccg tagtaattct
      841 gattgctgca gcgggtggtg tcgcgctgtt aacgacaaaa ggtaaagcta ccattgcttt
      901 tgcccgtgaa gcgcgtaccg aagtccgtaa ggtcatttgg ccgactcgcc aggaaacatt
      961 gcacaccacg ctgattgtgg ctgcggttac cgcagtaatg tcactgatcc tgtggggact
     1021 ggatggtatt ctggttcgcc tggtatcctt tatcactggc ctgaggttct gagatgtctg
     1081 aagctcctaa aaagcgctgg tacgtcgttc aggcgttttc cggttttgaa ggccgcgtag
     1141 caacgtcgct gcgtgagcat atcaaattac acaacatgga agatttgttt ggtgaagtca
     1201 tggtaccaac cgaagaagtg gttgaaatcc gtggcggtca gcgtcgcaaa agcgaacgta
     1261 aattcttccc tggctacgtc ctcgttcaga tggtgatgaa cgacgcgagc tggcacttgg
     1321 tgcgcagcgt accgcgtgtg atgggcttca tcggcggtac ttccgatcgt cctgcgccaa
     1381 tcagcgataa agaagtcgat gcgattatga accgcctgca gcaggttggt gataagccgc
     1441 gtccgaaaac gctgtttgaa ccgggtgaaa tggtccgtgt taatgatggt ccgttcgctg
     1501 acttcaacgg tgttgttgaa gaagtggatt acgagaaatc tcgtctgaaa gtgtctgttt
     1561 ctatcttcgg tcgtgcgacc ccggtagagc tggacttcag ccaggttgaa aaagcctaac
     1621 ccagcgatca aaaaagcggc gatttaatcg ttgcacaagg cgtgagattg gaatacaatt
     1681 tcgcgccttt tgtttttatg ggccttgccc gtaaaacgat tttttatatc acggggagcc
     1741 tctcagaggc gttattaccc aacttgagga atttataatg gctaagaaag tacaagccta
     1801 tgtcaagctg caggttgcag ctggtatggc taacccgagt ccgccagtag gtccggctct
     1861 gggtcagcag ggcgtaaaca tcatggaatt ctgcaaagcg ttcaacgcaa aaactgattc
     1921 catcgaaaaa ggtctgccga ttccggtagt aatcaccgtt tacgctgacc gttctttcac
     1981 tttcgttacc aagaccccgc cggcagcagt tctgctgaaa aaagcggcag gtatcaagtc
     2041 tggttccggt aagccgaaca aagacaaagt gggtaaaatt tcccgcgctc agctgcagga
     2101 aatcgcgcag accaaagctg ccgacatgac tggtgccgac attgaagcga tgactcgctc
     2161 catcgaaggt actgcacgtt ccatgggcct ggtagtggag gactaagaaa tggctaaact
     2221 gaccaagcgc atgcgtgtta tccgtgagaa agttgatgca accaaacagt acgacatcaa
     2281 cgaagctatc gcactgctga aagagctggc gactgctaaa ttcgtagaaa gcgtggacgt
     2341 agctgttaac ctcggcatcg acgctcgtaa atctgaccag aacgtacgtg gtgcaactgt
     2401 actgccgcac ggtactggcc gttccgttcg cgtagccgta tttacccaag gtgcaaacgc
     2461 tgaagctgct aaagctgcag gcgcagaact ggtaggtatg gaagatctgg ctgaccagat
     2521 caagaaaggc gaaatgaact ttgacgttgt tattgcttct ccggatgcaa tgcgcgttgt
     2581 tggccagctg ggccaggttc tgggtccgcg tggcctgatg ccaaacccga aagtgggtac
     2641 tgtaacaccg aacgttgctg aagcggttaa aaacgctaaa gctggccagg ttcgttaccg
     2701 taacgacaaa aacggcatca tccacaccac catcggtaaa gtggactttg acgctgacaa
     2761 actgaaagaa aacctggaag ctctgctggt tgcgctgaaa aaagcaaaac cgactcaggc
     2821 gaaaggcgtg tacatcaaga aagttagcat ctccaccacc atgggtgcag gtgttgcagt
     2881 tgaccaggct ggcctgagcg cttctgtaaa ctaatgcctt tacgtgggcg gtgattttgt
     2941 ctacaatctt acccccacgt ataatgctta atgcagacgt atatccgaga tattcgggtt
     3001 gtggcaaggc ggcaactgag tgagtcgcca agagcatagc taactatgtg actggtgcga
     3061 atgaaggaag ccaacgccgc cacaagttga atagcgacgg atagaaaaga tttgttcgtt
     3121 ggagcctggc ctatccaggc ctccgtcgaa gaccgcagga gtttcgcaag aaacttaatc
     3181 ccctgcgtag acggtgacag aacgctaaga ttattctttt atattctggc ttgtttctgc
     3241 tcaccgtaat taagacgctc tctccgtttg gaggagtgaa gtgagttcca gagattttct
     3301 ctggcaaaca tccaggagca aagctaatgg ctttaaatct tcaagacaaa caagcgattg
     3361 ttgctgaagt cagcgaagta gccaaaggcg cgctgtctgc agtagttgcg gattcccgtg
     3421 gcgtaactgt agataaaatg actgaactgc gtaaagcagg tcgcgaagct ggcgtataca
     3481 tgcgtgttgt tcgtaacacc ctgctgcgcc gtgctgttga aggtactccg ttcgagtgcc
     3541 tgaaagacgc gtttgttggt ccgaccctga ttgcatactc tatggaacac ccgggcgctg
     3601 ctgctcgtct gttcaaagag ttcgcgaaag cgaatgcaaa atttgaggtc aaagccgctg
     3661 cctttgaagg tgagctgatc ccggcgtctc agatcgaccg cctggcaact ctgccgacct
     3721 acgaagaagc aattgcacgc ctgatggcaa ccatgaaaga agcttcggct ggcaaactgg
     3781 ttcgtactct ggctgctgta cgcgatgcga aagaagctgc ttaatcgcag ttatcttttt
     3841 aacgcattcg cttacgtata aacttattct gatattcagg aacaatttaa atgtctatca
     3901 ctaaagatca aatcattgaa gcagttgcag ctatgtctgt aatggacgtt gtagaactga
     3961 tctctgcaat ggaagaaaaa ttcggtgttt ccgctgctgc tgctgtagct gtagctgctg
     4021 gcccggttga agctgctgaa gaaaaaactg aattcgacgt aattctgaaa gctgctggcg
     4081 ctaacaaagt tgctgttatc aaagcagtac gtggcgcaac tggcctgggt ctgaaagaag
     4141 ctaaagacct ggtagaatct gcaccggctg ctctgaaaga aggcgtgagc aaagacgacg
     4201 cagaagcact gaaaaaagct ctggaagaag ctggcgctga agttgaagtt aaataagcca
     4261 acccttccgg ttgcagcctg agaaatcagg ctgatggctg gtgacttttt agtcaccagc
     4321 ctttttgcgc tgtaaggcgc cagtagcgtt tcacactgtt tgactactgc tgtgcctttc
     4381 aatgcttgtt tctatcgacg acttaatata ctgcgacagg acgtccgttc tgtgtaaatc
     4441 gcaatgaaat ggtttaagcg tgatagcaac aggcattgcg gaaagtgttc cattttccgg
     4501 tcaacaaaat agtgttgcac aaactgtccg ctcaatggac agatgggtcg acttgtcagc
     4561 gagctgagga accctatggt ttactcctat accgagaaaa aacgtattcg taaggatttt
     4621 ggtaaacgtc cacaagttct ggatgtacct tatctccttt ctatccagct tgactcgttt
     4681 cagaaattta tcgagcaaga tcctgaaggg cagtatggtc tggaagctgc tttccgttcc
     4741 gtattcccga ttcagagcta cagcggtaat tccgagctgc aatacgtcag ctaccgcctt
     4801 ggcgaaccgg tgtttgacgt ccaggaatgt caaatccgtg gcgtgaccta ttccgcaccg
     4861 ctgcgcgtta aactgcgtct ggtgatctat gagcgcgaag cgccggaagg caccgtaaaa
     4921 gacattaaag aacaagaagt ctacatgggc gaaattccgc tcatgacaga caacggtacc
     4981 tttgttatca acggtactga gcgtgttatc gtttcccagc tgcaccgtag tccgggcgtc
     5041 ttctttgact ccgacaaagg taaaacccac tcttcgggta aagtgctgta taacgcgcgc
     5101 atcatccctt accgtggttc ctggctggac ttcgaattcg atccgaagga caacctgttc
     5161 gtacgtatcg accgtcgccg taaactgcct gcgaccatca ttctgcgcgc cctgaactac
     5221 accacagagc agatcctcga cctgttcttt gaaaaagtta tctttgaaat ccgtgataac
     5281 aagctgcaga tggaactggt gccggaacgc ctgcgtggtg aaaccgcatc ttttgacatc
     5341 gaagctaacg gtaaagtgta cgtagaaaaa ggccgccgta tcactgcgcg ccacattcgc
     5401 cagctggaaa aagacgacgt caaactgatt gaagtcccgg ttgagtacat cgcaggtaaa
     5461 gtggttgcta aagactatat tgatgagtct accggcgagc tgatctgcgc agcgaacatg
     5521 gagctgagct tggatctgct ggctaagctg agccagtctg gtcacaagcg tatcgaaacg
     5581 ctgttcacca acgatctgga tcacggccca tatatctctg aaaccttacg tgtcgaccca
     5641 actaacgacc gtctgagcgc actggtagaa atctaccgca tgatgcgccc tggcgagccg
     5701 ccgactcgtg aagcagctga aagcctgttc gagaacctgt tcttctccga agaccgttat
     5761 gacttgtctg cggttggtcg tatgaagttc aaccgttctc tgctgcgcga agaaatcgaa
     5821 ggttccggta ttctgagcaa agacgacatc attgatgtta tgaaaaagct catcgatatc
     5881 cgtaacggta aaggcgaagt cgatgatatc gaccacctcg gcaaccgtcg tatccgttcc
     5941 gttggcgaaa tggcggaaaa ccagttccgc gttggcctgg tacgtgtaga gcgtgcggtg
     6001 aaagagcgtc tgtctctggg cgatctggat accctgatgc ctcaggatat gatcaacgcc
     6061 aagccgattt ccgcagcagt gaaagagttc ttcggttcca gccagctgtc tcagtttatg
     6121 gaccagaaca acccgctgtc tgagattacg cacaaacgtc gtatctccgc actcggccca
     6181 ggcggtctga cccgtgaacg tgcaggcttc gaagttcgag acgtacaccc gactcactac
     6241 ggtcgcgtat gtccaatcga aacccctgaa ggtccgaaca tcggtctgat caactctctg
     6301 tccgtgtacg cacagactaa cgaatacggc ttccttgaga ctccgtatcg taaagtgacc
     6361 gacggtgttg taactgacga aattcactac ctgtctgcta tcgaagaagg caactacgtt
     6421 atcgcccagg cgaactccaa cctggatgaa gaaggccact tcgtagaaga cctggtaact
     6481 tgccgtagca aaggcgaatc cagcttgttc agccgcgacc aggttgacta catggacgta
     6541 tccacccagc aggtggtatc cgtcggtgcg tccctgatcc cgttcctgga acacgatgac
     6601 gccaaccgtg cattgatggg tgcgaatatg caacgtcagg ccgttccgac tctgcgcgct
     6661 gataagccgc tggttggtac tggtatggaa cgtgctgttg ccgttgactc cggtgtaact
     6721 gcggtagcta aacgtggtgg tgtcgttcag tacgtggatg cttcccgtat cgttatcaaa
     6781 gttaacgaag acgagatgta tccgggtgaa gcaggtatcg acatctacaa cctgaccaaa
     6841 tacacccgtt ctaaccagaa cacctgtatc aaccagatgc cgtgtgtgtc tctgggtgaa
     6901 ccggttgaac gtggcgacgt gctggcagac ggtccgtcca ccgacctcgg tgaactggcg
     6961 cttggtcaga acatgcgcgt agcgttcatg ccgtggaatg gttacaactt cgaagactcc
     7021 atcctcgtat ccgagcgtgt tgttcaggaa gaccgtttca ccaccatcca cattcaggaa
     7081 ctggcgtgtg tgtcccgtga caccaagctg ggtccggaag agatcaccgc tgacatcccg
     7141 aacgtgggtg aagctgcgct ctccaaactg gatgaatccg gtatcgttta cattggtgcg
     7201 gaagtgaccg gtggcgacat tctggttggt aaggtaacgc cgaaaggtga aactcagctg
     7261 accccagaag aaaaactgct gcgtgcgatc ttcggtgaga aagcctctga cgttaaagac
     7321 tcttctctgc gcgtaccaaa cggtgtatcc ggtacggtta tcgacgttca ggtctttact
     7381 cgcgatggcg tagaaaaaga caaacgtgcg ctggaaatcg aagaaatgca gctcaaacag
     7441 gcgaagaaag acctgtctga agaactgcag atcctcgaag cgggtctgtt cagccgtatc
     7501 cgtgctgtgc tggtagccgg tggcgttgaa gctgagaagc tcgacaaact gccgcgcgat
     7561 cgctggctgg agctgggcct gacagacgaa gagaaacaaa atcagctgga acagctggct
     7621 gagcagtatg acgaactgaa acacgagttc gagaagaaac tcgaagcgaa acgccgcaaa
     7681 atcacccagg gcgacgatct ggcaccgggc gtgctgaaga ttgttaaggt atatctggcg
     7741 gttaaacgcc gtatccagcc tggtgacaag atggcaggtc gtcacggtaa caagggtgta
     7801 atttctaaga tcaacccgat cgaagatatg ccttacgatg aaaacggtac gccggtagac
     7861 atcgtactga acccgctggg cgtaccgtct cgtatgaaca tcggtcagat cctcgaaacc
     7921 cacctgggta tggctgcgaa aggtatcggc gacaagatca acgccatgct gaaacagcag
     7981 caagaagtcg cgaaactgcg cgaattcatc cagcgtgcgt acgatctggg cgctgacgtt
     8041 cgtcagaaag ttgacctgag taccttcagc gatgaagaag ttatgcgtct ggctgaaaac
     8101 ctgcgcaaag gtatgccaat cgcaacgccg gtgttcgacg gtgcgaaaga agcagaaatt
     8161 aaagagctgc tgaaacttgg cgacctgccg acttccggtc agatccgcct gtacgatggt
     8221 cgcactggtg aacagttcga gcgtccggta accgttggtt acatgtacat gctgaaactg
     8281 aaccacctgg tcgacgacaa gatgcacgcg cgttccaccg gttcttacag cctggttact
     8341 cagcagccgc tgggtggtaa ggcacagttc ggtggtcagc gtttcgggga gatggaagtg
     8401 tgggcgctgg aagcatacgg cgcagcatac accctgcagg aaatgctcac tgttaagtct
     8461 gatgacgtga acggtcgtac caagatgtat aaaaacattg tggacggcaa ccatcagatg
     8521 gagccgggca tgccagaatc cttcaacgta ttgttgaaag agattcgttc gctgggtatc
     8581 aacatcgaac tggaagacga gtaattctcg ctcaaacagg tcactgctgt cggggtaaaa
     8641 cccggcagcg gattgtgcta actccgacgg gagcaaatcc gtgaaagatt tattaaagtt
     8701 tctgaaagcg cagactaaaa ccgaagagtt tgatgcgatc aaaattgctc tggcttcgcc
     8761 agacatgatc cgttcatggt ctttcggtga agttaaaaag ccggaaacca tcaactaccg
     8821 tacgttcaaa ccagaacgtg acggcctttt ctgcgcccgt atctttgggc cggtaaaaga
     8881 ttacgagtgc ctgtgcggta agtacaagcg cctgaaacac cgtggcgtca tctgtgagaa
     8941 gtgcggcgtt gaagtgaccc agactaaagt acgccgtgag cgtatgggcc acatcgaact
     9001 ggcttccccg actgcgcaca tctggttcct gaaatcgctg ccgtcccgta tcggtctgct
     9061 gctcgatatg ccgctgcgcg atatcgaacg cgtactgtac tttgaatcct atgtggttat
     9121 cgaaggcggt atgaccaacc tggaacgtca gcagatcctg actgaagagc agtatctgga
     9181 cgcgctggaa gagttcggtg acgaattcga cgcgaagatg ggtgcggaag caatccaggc
     9241 tctgctgaag agcatggatc tggagcaaga gtgcgaacag ctgcgtgaag agctgaacga
     9301 aaccaactcc gaaaccaagc gtaaaaagct gaccaagcgt atcaaactgc tggaagcgtt
     9361 cgttcagtct ggtaacaaac cagagtggat gatcctgacc gttctgccgg tactgccgcc
     9421 agatctgcgt ccgctggttc cgctggatgg tggtcgtttc gcgacttctg acctgaacga
     9481 tctgtatcgt cgcgtcatta accgtaacaa ccgtctgaaa cgtctgctgg atctggctgc
     9541 gccggacatc atcgtacgta acgaaaaacg tatgctgcag gaagcggtag acgccctgct
     9601 ggataacggt cgtcgcggtc gtgcgatcac cggttctaac aagcgtcctc tgaaatcttt
     9661 ggccgacatg atcaaaggta aacagggtcg tttccgtcag aacctgctcg gtaagcgtgt
     9721 tgactactcc ggtcgttctg taatcaccgt aggtccatac ctgcgtctgc atcagtgcgg
     9781 tctgccgaag aaaatggcgc tggagctgtt caaaccgttc atctacggca agctggaact
     9841 gcgtggtctt gctaccacca ttaaagctgc gaagaaaatg gttgagcgcg aagaagctgt
     9901 cgtttgggat atcctggacg aagttatccg cgaacacccg gtactgctga accgtgcacc
     9961 gactctgcac cgtctgggta tccaggcatt tgaaccggta ctgatcgaag gtaaagctat
    10021 ccagctgcac ccgctggttt gtgcggcata taacgccgac ttcgatggtg accagatggc
    10081 tgttcacgta ccgctgacgc tggaagccca gctggaagcg cgtgcgctga tgatgtctac
    10141 caacaacatc ctgtccccgg cgaacggcga accaatcatc gttccgtctc aggacgttgt
    10201 actgggtctg tactacatga cccgtgactg tgttaacgcc aaaggcgaag gcatggtgct
    10261 gactggcccg aaagaagcag aacgtctgta tcgctctggt ctggcttctc tgcatgcgcg
    10321 cgttaaagtg cgtatcaccg agtatgaaaa agatgctaac ggtgaattag tagcgaaaac
    10381 cagcctgaaa gacacgactg ttggccgtgc cattctgtgg atgattgtac cgaaaggtct
    10441 gccttactcc atcgtcaacc aggcgctggg taaaaaagca atctccaaaa tgctgaacac
    10501 ctgctaccgc attctcggtc tgaaaccgac cgttattttt gcggaccaga tcatgtacac
    10561 cggcttcgcc tatgcagcgc gttctggtgc atctgttggc atcgatgaca tggtcatccc
    10621 ggagaagaaa cacgaaatca tctccgaggc agaagcagaa gttgctgaaa ttcaggagca
    10681 gttccagtct ggtctggtaa ctgcgggcga acgctacaac aaagttatcg atatctgggc
    10741 tgcggcgaac gatcgtgtat ccaaagcgat gatggataac ctgcaaactg aaaccgttat
    10801 taaccgtgac ggtcaggaag agaagcaggt ttccttcaac agcatctaca tgatggccga
    10861 ctccggtgcg cgtggttctg cggcacagat tcgtcagctt gctggtatgc gtggtctgat
    10921 ggcaaagccg gatggctcca tcatcgaaac gccaatcacc gcgaacttcc gtgaaggtct
    10981 gaacgtactc cagtacttca tctccaccca cggtgctcgt aaaggtctgg cggataccgc
    11041 actgaaaact gcgaactccg gttacctgac tcgtcgtctg gttgacgtgg cgcaggacct
    11101 ggtggttacc gaagacgatt gtggtaccca tgaaggtatc atgatgactc cggttatcga
    11161 ggggggtgac gttaaagagc cgctgcgcga tcgcgtactg ggtcgtgtaa ctgctgaaga
    11221 cgttctgaag ccgggtactg ctgatattct cgttccgcgc aacacgctgc tgcacgaaca
    11281 gtggtgtgac ctgctggaag agaactctgt cgacgcggta aaagtacgtt ctgttgtatc
    11341 ttgtgacacc gactttggtg tatgtgcgca ctgctacggt cgtgacctgg cgcgtggcca
    11401 catcatcaac aaaggtgaag caatcggtgt tatcgcggca cagtccatcg gtgaaccggg
    11461 tacacagctg accatgcgta cgttccacat cggtggtgcg gcatctcgtg cggctgctga
    11521 atccagcatc caggtgaaaa acaaaggtag catcaagctc agcaacgtga agtcggttgt
    11581 gaactccagc ggtaaactgg ttatcacttc ccgtaacacc gaactgaaac tgatcgacga
    11641 attcggtcgt accaaagaaa gctacaaagt gccttacggt gcggtactgg cgaaaggcga
    11701 tggcgaacag gttgctggcg gcgaaaccgt tgcaaactgg gacccgcaca ccatgccggt
    11761 tatcaccgaa gtaagcggtt ttgtacgctt tactgacatg atcgacggcc agaccattac
    11821 tcgtcagacc gacgaactga ccggtctgtc ttcgctggtg gttctggatt ccgcagaacg
    11881 taccgcaggt ggtaaagatc tgcgtccggc actgaaaatc gttgatgctc agggtaacga
    11941 cgttctgatc ccaggtaccg atatgcctgc gcagtacttc ctgccgggta aagcgattgt
    12001 tcagctggaa gatggcgtac agatcagctc tggtgacacc ttggcgcgta ttccgcagga
    12061 atccggcggt accaaggaca tcaccggtgg tctgccgcgc gttgcggacc tgttcgaagc
    12121 tcgtcgtccg aaagagccgg caatcctggc tgaaatcagc ggtatcgttt ccttcggtaa
    12181 agaaaccaaa ggtaaacgtc gtctggttat caccccggta gacggtagcg atccgtacga
    12241 agagatgatt ccgaaatggc gtcagctcaa cgtgttcgaa ggtgaacgtg tagaacgtgg
    12301 tgacgtaatt tccgacggtc cggaagcgcc gcacgacatt ctgcgtctgc gtggtgttca
    12361 tgctgtgact cgttacatcg ttaacgaagt acaggacgta taccgtctgc agggcgtaaa
    12421 gattaacgat aaacacatcg aagttatcgt tcgtcagatg ctgcgtaaag ctaccatcgt
    12481 taacgcgggc agctccgact tcctggaagg cgaacaggtt gaatactctc gcgtcaagat
    12541 cgcaaaccgc gaactggaag cgaacggcaa agtgggtgca acttactccc gcgatctgct
    12601 gggtatcacc aaagcgtctc tggcaaccga gtccttcatc tccgcggcat cgttccagga
    12661 gaccactcgc gtgctgaccg aagcagccgt tgcgggcaaa cgcgacgaac tgcgcggcct
    12721 gaaagagaac gttatcgtgg gtcgtctgat cccggcaggt accggttacg cgtaccacca
    12781 ggatcgtatg cgtcgccgtg ctgcgggtga agctccggct gcaccgcagg tgactgcaga
    12841 agacgcatct gccagcctgg cagaactgct gaacgcaggt ctgggcggtt ctgataacga
    12901 gtaatcgtta atccgcaaat aacgtaaaaa cccgcttcgg cgggtttttt tatgggtgtt
    12961 attcagaggg caaattcttc acggaaaata ttaagaaagg cagggcgaaa cgaggaagaa
    13021 gcttttcgcc ctggacaaca ttcctgctga cggcactacc ataaaaaaac acaacgcttt
    13081 caacaacctt gcgtttttca tcttccgtag cggctcggaa taaatttacg ttcttacatt
    13141 ccgtctcata gtctttgcga ggcgcgtccc agatagctgt cccagtcttt ccataccggc
    13201 tgcaaaccct gagcggttaa cacggcagca accgcttccg gtctgcgatc gtcgtgcggt
    13261 gagaactgtt ccaactcggg gtgattatcg gcatagccac ctggctgcgt tttcgagaag
    13321 gcgctgacgt tattgatcgc cagcggaata acgcgatcgc gaaaccacgg tgattcccgc
    13381 gtggagagtg acagttcaat ctccggtgca agcagtcgga aggcgcagat ggtttgcact
    13441 aactggcgtt catccataat cgacgcaggc tcaatgccgc cagtacacgg gcgcaggcgc
    13501 ggaaaggaga cagagtaacg gctttgccag taatgctgtt gcagccatag caaatgttcg
    13561 gcaaccatat agcagtcaac gcgccagttg tcggaaaggc caattagcgc gccgaggcct
    13621 atcttatcaa tccccgcacg ccccagccga tccggcgttt ccagccgcca gaagaagtcc
    13681 tgttttttgc ctttcaaatg atggcgggta taagtcgcct cgtgatatgt ctcctgataa
    13741 accatcaccc catccagacc aagttgcttt aactcggcgt attccgtctc cgccagcggt
    13801 tgcacttcca tttgtagtga agagaactgt tcacgcaggg cggggagatg acgacgaaag
    13861 taatccatcc ccactttcgc ctgatgttca ccagtgacta atagcagatg ttcaaagccc
    13921 atctcccgta tagcggcgct ttccctggca atatccgctt catccagcgt tttgcgcttg
    13981 atgcgattac tcatggaaaa tccgcagtac gtgcagtcgt tagcgcaaag attggaaaga
    14041 taaagcggga cgtagaaact aactgtgttg ccaaatcgct gacgggtcag acgctgcgcc
    14101 tgttgggcca gttgttccag atagccactg gcggcaggcg ataacagcgc catcatgtcg
    14161 tcgcgggtga gttgcgaggc atttagcgcc cgctctacgt cagcagccgt tttgccgttg
    14221 atacgcaggc ggatgtcgtc ccagtccagt tgtcgccagc gattgctgaa ggttttcatg
    14281 ccgatgcctc cagaaatccg gtcagcgggc tggtggcatg agcaaaataa ctgcggctgc
    14341 ccggtccgga ctgacgtgcc agcaggccta cttctaccgc cagacgaaat gccttcgcca
    14401 tgttgacggg atcgtccgcg acggcaatcg ccgtattcac taacaccgcg tcggccccca
    14461 tttccagcgc ctgcgtggca tggctgggaa cgccgatgcc agcatcgaca accaccggaa
    14521 ctgtggcctg ctggataata atctccagca tggcacgggt ttccagtccc tgattcgagc
    14581 caatcggcgc gccgagcggc atcaccgctg cacagccgac ttcttccaga cgtttacaca
    14641 ataccggatc ggccccgcag taaggcagca cgacaaatcc ctgttgtacc agcgtttcgg
    14701 cggctttcag ggtttcgatg ggatcgggca acagccagcg ggcgtcaggg tgaatctcca
    14761 attttagcca gtttgtgcct aacgcttcac gtgccagatg ggcggcgaaa atggcttctt
    14821 ccgctgtttt cgccccggaa gtattcggca gcagggccac acccgccgcg ataagcggtt
    14881 cgaggatagc gtcgttgtgc tggcgcaagt cgacacgttt catcgccagt gtcaccagct
    14941 ggctgccgga agcgcggatc gcctccacca tcagttgtga agaagcgaat ttccctgtgc
    15001 cggtaaacag atgtgaatca aacgttttgt ccgcaatacg taacatctca accccctgca
    15061 ataacctgaa aaagcaggat ctggtcgcca tcctgcacga tatgttgcgt ccactgctca
    15121 cgcgggacga tttgctgatt aatcgccaga gccgcgcccg cttgtcgttg gtcgagttgc
    15181 tccagtagtt cgtgaacagt ttgcccggcg gcgcactgca tcggttgatc gttaaacagg
    15241 atctgcattg cttcctccgc ataccgggca accactggcg cggcgcaacg ccaggctgcg
    15301 ccactggctc gacttaccgt cgaacagtcg gagttctccc gcaggcgtct ctataccgct
    15361 taataactta atggcttcca gtgcctgcaa agtgcccatc accccgacca ccgggccaac
    15421 cacgcccgcc gtgcggcagt tgcgttctgg ctgctggtta tctggccaca ggcagcggta
    15481 acacccctgc tcccagggcg gtgtcagtac catcaactga ccgccaaatc cgaccgcgct
    15541 ggcggtgata agcggcgtgt tgagtgccac gcaggcggca ttaatctcct ggcgagtcgc
    15601 catattgtcg gtacagtcga gcaccacatc ggcttgtgca accgcatctt tcaacgcctc
    15661 acccgttaac cgttgttgta atgctgtcag ttgaatgtcg ggattcaact gtgtcagtcg
    15721 ctgttggctg acctgcgatt tcgggctgtt gatatcttca gtggtaaaga ggatttgtcg
    15781 ttgcagattg cttaaatgca catcgtcgtc atctgccagt accagcgtcc cgacgccagc
    15841 ccctgccagg tatagcgcag caggtgtacc cagcccgcca agaccaataa tcagcacctg
    15901 gctgtcgagc agtttttgct gcccgtcaag agcgatatcg tcgagcagaa tttggcggct
    15961 ataacgcata aagtcacggt cattcatcgc caactcctgc aatttccagc aactgtgccg
    16021 ttgccaaacg ccagtctgcg gcttgagtaa tggcgctgac gacggcgata ctgccgacac
    16081 ccgttgctat caccgcaggc gcgcgtgcca gactgatacc gccaatcgcc acggtgggat
    16141 aatccgccag gcgctcaaca tgccgtgcca actgttccag cccctgcggt gcagaaggca
    16201 tctgtttggt ttgcgtcggg aacacatgtc ccagcgcgat ataagagggg cgtgctgcca
    16261 gcgcgacgtc gatttccata tcgtcatgtg tcgaaacgcc cagccgcagg cctgccgcgc
    16321 ggatggcatt gagatcggtg gcttgcaaat cttcctgccc caaatggacg ccatacgcct
    16381 gatgcttgat cgcgagccgc cagtaatcgt tgataaacaa tcgcgcgtta tagcggcggc
    16441 ccagcgcaat tgccgccacg acatcggctt ccacctcttc atcgcgccga tctttgatgc
    16501 gtagctggag agtacgtacg cctgcatcca acagacgttc gatccactgt acgctgtcca
    16561 ccaccgggta cagtcctaaa cgaaaaggta caggaggaaa atcaggctga tacatcacgc
    16621 ttcctcctta tgcaggtaga tttctccgcc tctggcacgg aagttctccg acatatccgc
    16681 cattcccact tcaatggttt gcgcggcggc gtaatcgcgt acttcctggc tgattttcat
    16741 cgagcagaat tttggcccac acatggagca aaaatgggcg actttgccgg actcttgggg
    16801 cagggtttca tcgtgataag cgcgggcggt aaacgggtcg agggccagat taaactggtc
    16861 ttcccagcga aattcgaagc gggctttcga catggcgtta tcgcgaattt gcgcgcccgg
    16921 atgccctttt gccaggtcag cagcgtgggc ggcaatctta taagtgataa gcccctgctt
    16981 aacatcttct ttattgggca gacccagatg ctcttttggc gttacgtaac agagcatcgc
    17041 gcagccaaac cagccaatca tcgccgcacc aatccccgac gtgaagtggt catagcccgg
    17101 tgcaatatcg gtagttagcg gccccagagt gtaaaacggc gcttcgtggc agtgctctaa
    17161 ctcctcggtc atattgcggc ggatcatctg catcggcaca tggcctgggc cttcaatcat
    17221 cacctgtacg tcatattccc aggcgatttt ggtcagttcg cccagcgtat gcaattcggc
    17281 aaactgcgct tcatcgttgg cgtcctgaat agaaccgggg cgcagaccgt cgcccagcga
    17341 cagcgaaacg tcataagcgg cacagatttc acaaatttcg cggaagtgtt gatagaggaa
    17401 attttcctga tgatgggaga ggcaccattt cgccataatc gaaccgccgc gagagacgat
    17461 accggtcagg cgtttcgcgg tcatcggcac atagcgcagc agtacgcccg catggatagt
    17521 gaagtaatcc acaccttgct cggcctgttc cagcagcgtg tcgcggaacg cttcccaggt
    17581 aagatcttcg gcgatcccgt taaccttctc cagcgcctgg tagatcggca ctgtaccgat
    17641 cggcaccgga ctgttacgca aaatccactc gcgggtttcg tgaatatagc gaccggtgga
    17701 gagatccatc accgtatccg ctccccagcg cgtggaccat accagctttt ccacttcttc
    17761 ttcgatggaa gaggtgaccg ccgagttgcc gatattggcg ttaactttta ccaggaagtt
    17821 acggccaata atcatcggct ccgattccgg atgattaatg ttggccggga taatcgcacg
    17881 tccggcagca acttcatcac ggacaaattc cgcagtgata ttttccggca gatgtgcgcc
    17941 aaagctcatt cccggatgct ggtggcgtaa aacctcgcta cggatgcgct cgcggcccat
    18001 attctcgcgg atggcgatga attccatttc cggcgtgatg atgccctggc gggcgtagtg
    18061 cagttgggtg acacggcgtc ctgttttggc gcgttttggc gttaataagc cgctaaaacg
    18121 tagctcgtcg agaccatcat ctgccagccg cgttctggtg taatcggaac tgcgcacggt
    18181 aagttcttcg gtatcggcgc gcgcatcgat ccacggctgg cgtagttttg ccagcccttg
    18241 ctgcacgtta atggcgatct gcggatcacc atacgggccg gaggtgtcgt agaccggaat
    18301 cgcttcgttt tcttcgtact gcggctgttc tttgctaccg ccaattagcg tcgggctaag
    18361 ctggatctca cgcatcggca cgcgcacgcc ggggtgtgtg ccagtgatat aaatgcgttt
    18421 tgagttggga aaggcggtgc cttccagggt gtcgataaaa tgttgggccc gggcgcgttg
    18481 ttcgcggcgg gtcagttttg ttgcagacat agctcattcc aaaaagttaa ggacgtggct
    18541 tgtcagacga cggatgaagc aagagacgat cgccgcaaag gcgatgcgat agcagattaa
    18601 ctcttgttcc cttcgcaggt attagcctga tcaggttccg cggatcccga ataaacggtc
    18661 tcagccagtt aaggcactcc gacaagaaat tagcccccac aaacggggcg ttgaatgtaa
    18721 attacgcgtt aacagcgcag aactcaagca ggatgtttga cgcgggcggc gtcaagcacc
    18781 agcagaatca gcttatcttc cagcacaaag cgcgcttcca gcgcctcgcc gatgtcagat
    18841 aaaacctgtt gaaactcaag gtaattatca tgatcgatgg cggtttccag gctggaatcg
    18901 tagtaatcca taatctgttg ggtattggct tcgagttgcg gccaaatctt cgcggcgcgc
    18961 gcgagttgcc cgttgccttc cagcttatga agaatgcgct cataaatact gaaatgtccg
    19021 gcagacaagt aatcgaccag gctctgacaa aaatcatcaa gggctttttc gtttagcctc
    19081 atgtacgatt ctttgccagg cttaatgcca accagattgt agtaagccac gagcagatgc
    19141 ttacgtacat gtagccagcg atcaaccagt ttgttacttc ctctgacgcg ctccgtcagg
    19201 ttatcgagct ggttaagcat gattgactcc gcaagtttgt attcaaaaac tgctcagtga
    19261 gaaatgtaaa aaccatgtta aacatgccag tgatgcaaag gtagtgcaag agctatggat
    19321 cgtataattg aaaaattaga tcacggctgg tgggtcgtca gccatgaaca aaaattatgg
    19381 ttgccgaagg gagaattgcc atatggcgaa gcggcaaatt tcgatcttgt gggtcagcgc
    19441 gcactacaaa tcggcgaatg gcagggggaa cctgtttggt taatacaaca gcagcggcgt
    19501 cacgatatgg ggtcggtacg tcaggtcatt gatctcgatg ttgggctgtt tcaactggcc
    19561 ggacgtggcg tgcaactggc ggagttttac cgatcgcata aatactgtgg ttactgcggg
    19621 catgaaatgt atccgagcaa aaccgaatgg gcgatgctgt gtagccattg ccgtgagcgt
    19681 tactacccgc aaatcgcccc ctgcattatt gttgccatcc gtcgcgatga ttcgctcctc
    19741 ctcgcccagc atacccgcca tcgtaacggt gtccatacag tacttgccgg attcgtcgaa
    19801 gtgggcgaaa ccctcgagca ggcagtcgcg cgggaagtga tggaagagag cggaattaaa
    19861 gttaaaaact tgcgttacgt gacttctcag ccgtggccgt ttcctcagtc tttaatgacc
    19921 gcgtttatgg cggaatatga cagcggcgac atcgtgatcg acccgaaaga attgctcgag
    19981 gcgaactggt atcgctatga cgatttgccg ttactcccgc cgcccggcac cgtagcgcgc
    20041 cgtctgatag aagatactgt ggcgatgtgt cgggcagagt atgagtgatg atacactgac
    20101 cgcctgacgc actaaggaac agccaaaatg accgaactta aaaacgatcg ttatctgcgg
    20161 gcgctgctgc gccagcccgt tgatgtcact ccagtatgga tgatgcgcca ggcgggtcgc
    20221 tatctaccgg aatataaagc cacgcgcgcc caggcgggcg attttatgtc gctgtgcaaa
    20281 aacgccgagc tggcgtgcga agtgactttg caaccgctgc gtcgctaccc gctggatgcg
    20341 gcgatcctct tttccgatat cctcaccgtg ccggacgcga tggggttagg gctctatttt
    20401 gaagccggag aaggtccgcg ttttacctcg ccagtcacct gcaaagccga cgtcgataaa
    20461 ctgccaattc cggacccgga agatgagctg ggttacgtga tgaacgcggt gcgtaccatt
    20521 cgtcacgaac tgaaaggcga agtgccgctg attggttttt ccggcagccc gtggacgctg
    20581 gcaacctaca tggtggaagg cggcagcagc aaagccttca ccgtgatcaa aaaaatgatg
    20641 tatgccgatc cgcaggcgct gcacgctctg ctcgataaac tggcgaaaag cgtcactttg
    20701 tatctgaatg cgcagattaa agccggtgct caggcagtga tgattttcga cacctggggc
    20761 ggcgtgctta ccgggcgcga ttatcaacag ttctcgctct attacatgca taaaattgtt
    20821 gatggtttac tgcgtgaaaa cgacggtcgc cgcgtgccag tcacgctgtt taccaaaggc
    20881 ggcggacagt ggctggaagc catggctgaa accggttgcg atgcgctggg cctcgactgg
    20941 acaacggaca tcgccgatgc gcgccgccgt gtgggcaata aagtcgcgtt gcagggtaat
    21001 atggatccgt cgatgctgta cgcgccgcct gcccgcattg aagaagaagt agcgactata
    21061 cttgcaggtt tcggtcacgg cgaaggtcat gtctttaacc ttggtcacgg cattcatcag
    21121 gatgtgccgc cagaacatgc tggcgtgttc gtggaggcag tgcatcgact gtctgaacag
    21181 tatcaccgct aagagcctga tccatcaggc tattttattt gccattttga ggccgggcag
    21241 tgctcaaaat cctcacgtac tacgtgtacg ctccggtttt tccgcgctgt ccggcctcaa
    21301 actgccttca acaattacgc ctgttgaacc aggctctaag gagcgattat ggatctcgcg
    21361 tcattacgcg ctcaacaaat cgaactggct tcttctgtga tctgcgagga tcgactcgat
    21421 aaagatccac cggatctgat cgccggagcc gatgtcgggt ttgagcaggg cggagaagtg
    21481 acgcgagcgg cgatggtgct gctgaaatat ccctcgcttg agctggtgga gtataaagtt
    21541 gcccgcatcg ccaccaccat gccttacatt ccaggttttc tttccttccg cgaatatcct
    21601 gcgctgctgg cagcgtggga gatgctgtcg caaaagccgg atttagtgtt tgtcgatggt
    21661 catgggatct cgcatcctcg ccgtcttggc gtcgccagcc attttggctt attggtggat
    21721 gtgccgacca ttggcgtggc gaaaaaacgg ctctgcggta aattcgaacc gctctccagc
    21781 aaaccgggcg cgctggcccc actgatggat aaaggcgagc agctggcctg ggtctggcgc
    21841 agcaaagcgc gctgtaaccc gttgtttatc gctaccggcc atcgggtcag cgtggacagc
    21901 gcgctggcgt gggtacaacg ctgcatgaaa ggctatcgtc tgccggagcc aacgcgctgg
    21961 gcggacgcgg tggcctcgga acgtccggcg ttcgtgcgct atacagcaaa tcagccctaa
    22021 ttcaggtaaa ctgcggataa tttccgtatt tgagaactca acatgttaca aaacccaatt
    22081 catctgcgtc tggagcgcct ggaaagctgg cagcatgtca ctttcatggc ttgcttatgc
    22141 gaacgcatgt accccaatta cgccatgttc tgccagcaaa ccggttttgg tgatgggcaa
    22201 atttaccgtc gtattctcga tctcatctgg gaaacgctga ccgttaaaga cgcaaaagta
    22261 aatttcgaca gccaactgga gaaatttgaa gaagcgattc cttcagccga cgatttcgat
    22321 ctgtacggcg tttatccggc aatcgatgcc tgcgtggcgt taagtgaact ggtccattcg
    22381 cgtttgagtg gtgaaacgct cgaacacgcg gtggaagtga gtaagacctc catcacgacc
    22441 gttgcgatgc tggaaatgac tcaggctggt cgcgaaatgg gcgatgaaga gcttaaagaa
    22501 aacccagctg tagagcaaga atgggacatt cagtgggaaa tattccgact tttagctgag
    22561 tgcgaagaac gcgatatcga gctgataaaa ggccttaggg cagacctgcg tgagtcgggt
    22621 gagagcaata ttggtataat ttttcagcaa taagaccaga aaacgtgatt taacgcctga
    22681 tttgtcgtac ctggagtctt ccctttcgcc ccccgtctgg tctacatttg gggggcgaaa
    22741 aaaagtggct atcggtgcgt gtatgcagga gagtgctatt ctggcatttc cgtcgcactc
    22801 gatgcttagc aagcgataaa cacattgtaa ggataactta tgaacaagac tcaactgatt
    22861 gatgtaattg cagagaaagc agaactgtcc aaaacccagg ctaaagctgc tctggagtcc
    22921 actctggctg caattactga gtctctgaaa gaaggcgatg ctgtacaact ggttggtttc
    22981 ggtaccttca aagtgaacca tcgcgctgag cgtactggcc gcaacccgca gaccggtaaa
    23041 gaaatcaaaa ttgccgcagc taacgtaccg gcatttgttt ctggcaaggc actgaaagac
    23101 gcagttaagt aagattgcgt ggcagtgaac agttttaacg aaggggtggt ttcacccctt
    23161 ttgtctttct ggcgtcgatc attgatgctg gcaggtgcgc tgtttctcac tgcctgtagc
    23221 cacaactctt cacttcctcc ctttaccgcc agtggatttg ctgaagacca gggcgcggta
    23281 cgcatctggc gaaaagacag cggcgataat gtgcatctgc ttgccgtgtt tagcccgtgg
    23341 cgcagtggcg ataccacgac gcgagagtat cgctggcagg gcgataacct cacgctcatc
    23401 aatatcaatg tttacagcaa accgccggtg aacattcgcg cgcgttttga cgatcgcggc
    23461 gatctgagct ttatgcaacg tgaatccgat ggggaaaagc agcagctttc taacgaccaa
    23521 atcgatttat accgttatcg tgctgatcag atccgccaga ttagcgatgc cttacgtcag
    23581 gggagagtcg tgctgcgcca ggggcgctgg catgcgatgg aacagaccgt gaccacctgc
    23641 gaagggcaaa ccattaaacc tgatttagat tcgcaggcga tagcgcatat cgagcgccgc
    23701 cagagccgct cttctgttga tgtcagcgtg gcatggctgg aagcgcccga aggttcgcaa
    23761 ttactgttag tggcaaactc tgatttctgt cgctggcaac ccaacgagaa aacgttctga
    23821 tttaccagtg gcccataccc atatgaccgc caccaccgca gccgccgtag cccatgccca
    23881 ttccggctcc gcgcggaata cccgcttcag ccatcgcgat atctcgtttc acccgtaact
    23941 catctaacga ctgacgcaaa ttctccatct ctttggcgac cgcgttaatt ttgctgctat
    24001 ccggtgggtt cgcggctaac agggcattgt attcataacg cttcgtcacc agttgctgtt
    24061 gcagtgcgct gctttgagcg taaaagtcat tatggatttt ctgccacgct gtctgttgtt
    24121 cgctggtcaa aggcgcggca ttttgctgcc acataccgtg tccgccgtga gcaaatgcag
    24181 atgtcgatcc catcgccatt gctgaaagcg ccatcattac cagggcaatt ttcgtgttcc
    24241 gtttcatggt taatcctcca gtggttgtct tacttcgggt attgcatctt ccgtgccaac
    24301 gatgaaacgc tgatatgacg ggtaatctgg catgataaac gagtaaaaat gactcgcctg
    24361 ctgcgggtag cgagtcattt ttactcattg aaacttaagc ctttgtgtta cagcgcaggg
    24421 taagcgctga taaaagatgg catgatttct gctgtcagaa agggatgagc aggcaaagaa
    24481 gaagatgcgt tttatgcaac gttctaaaga ctccttagct aaatggttaa gcgcgatcct
    24541 ccccgtggtc attgttgggc tggtagggtt gtttgcggtg acggtgattc gcgattatgg
    24601 gcgcgagact gccgccgcca gacaaacgct gctggaaaaa ggcagtgtac ttattcgcgc
    24661 tcttgaatcc ggctcgcgcg tcggcatggg gatgcgcatg catcatgcgc agcagcaggc
    24721 cttactggaa gaaatggccg ggcagcctgg agtacgttgg tttgcggtca cggatgaaca
    24781 aggaacaatc gtgatgcata gcaactccgg catggtggga aaacagcttt attcccctca
    24841 ggaaatgcag cagttacatc cgggagatga agaagcgtgg cggcggatcg atagcgcaga
    24901 cggcgagcct gttctggaaa tttatcgcca gtttcaaccg atgtttgctg ctggaatgta
    24961 ccggatgcgc catatgcagc agtatgccgc gacaccacaa gcaattttca tcgctttcga
    25021 cgccagtaat attgtgagtg ccgaagatcg tgagcagaga aacaccctga ttattctctt
    25081 cgccctggcg acggtcttgc tggcaagcgt attgtcattc ttctggtatc gccgctatct
    25141 gcgctcgcgc cagcttctac aagatgaaat gaagcgcaaa gagaagctgg tggcgctggg
    25201 gcatcttgcg gcaggcgttg cccacgaaat ccgtaaccca ctttcctcga ttaaaggact
    25261 ggcgaaatac tttgccgagc gcgcgcctgc agggggagaa gcgcatcaac tggcgcaggt
    25321 gatggcgaaa gaggccgacc gtttaaaccg cgtggtaagc gagttgctgg aactggttaa
    25381 gccaacgcat ctggctttgc aggcggtgga tctcaacacg ctgattaacc actcattaca
    25441 gctggtaagc caggatgcaa acagccggga gatccagtta cgctttaccg ccaacgacac
    25501 attaccggaa attcaggccg atccggacag gctgactcag gtcctgttga atctctatct
    25561 caatgctatt caggcgattg gtcagcatgg cgtgattagc gtgacggcca gcgaaagcgg
    25621 cgcgggcgtg aaaatcagcg ttaccgacag cggtaaggga attgcggcag atcagcttga
    25681 agccatcttc actccgtact tcaccaccaa agccgaaggc accggattgg ggctggcggt
    25741 cgtgcataat attgttgaac aacacggtgg tacaattcag gtcgcaagcc aggagggaaa
    25801 aggctcaacg ttcaccctct ggcttccggt caatattacg cgtaaggacc cacaaggatg
    25861 acgcacgata atatcgatat tctggtggtg gatgatgaca ttagccactg cactattttg
    25921 caggctttac tgcgcggctg gggctataac gtcgcgctgg cgaacagcgg gcgacaggcg
    25981 ctggagcagg tgcgggaaca ggtttttgat cttgtgcttt gcgatgtgcg aatggcggag
    26041 atggacggca tcgccacgct gaaagagatc aaagcgttaa acccggcaat tccggtgctg
    26101 attatgactg cgtactccag cgtcgagacg gcggtagagg cactgaaaac tggggcgcag
    26161 gattatctca tcaagccgct ggatttcgat aacctacagg cgacgctgga aaaggcgctc
    26221 gcgcatacgc acagtattga tgctgaaaca cctgcggtga ctgccagcca gttcggtatg
    26281 gtcggtaaaa gcccggcgat gcaacacctg ctcagtgaaa tcgccctcgt cgcgccatcg
    26341 gaagccacgg tgctgatcca cggcgattcc ggcaccggta aagagctggt cgccagggcg
    26401 attcacgcca gtagcgcacg tagcgaaaaa ccgctggtaa cgctcaactg tgcggcactc
    26461 aacgaatcct tgctggaatc tgaattgttc ggtcacgaaa aaggggcgtt tactggagcc
    26521 gataaacgcc gggaagggcg ctttgttgag gcggacggcg gcacgctgtt tctcgatgaa
    26581 attggcgata tctcgccgat gatgcaggtg cgtctgctgc gtgcgattca ggagcgcgaa
    26641 gttcagcgtg tcggtagcaa ccagactatc tcggttgatg tccggctgat tgcggcgacc
    26701 catcgcgatc ttgccgcaga ggtgaatgcc gggcgttttc gccaggatct ctactatcgc
    26761 ctgaatgtgg tggcgattga agtaccatcg ctgcggcaac ggcgggaaga tatccctctg
    26821 ctggctggcc attttcttca gcgctttgcc gagcgtaatc gcaaggcggt aaaaggcttt
    26881 acgccacagg cgatggattt gctgatccac tacgactggc cgggaaatat tcgtgaactg
    26941 gaaaacgcgg tggaacgggc ggtggtgctg ctgaccgggg aatatatttc cgaacgcgag
    27001 ctgccgctgg cgattgccag tacgccgatc ccgctggggc aaagtcagga tattcagccg
    27061 ctggtggaag tggaaaaaga ggtgattctg gcggcgctgg agaaaacggg cggcaacaaa
    27121 accgaagccg cccgtcagtt agggatcacg cgcaaaacgc tattggcaaa actgtcgcgt
    27181 tagttctgct cgcgttcgat ggcgcgccaa ccgatatctt tccggcagaa gcaatcatcc
    27241 cagtggatat cggtcattaa ggcgtaggcg cgtttctgcg cttctgccac ggtatgaccc
    27301 agcgcggtga cgcacagtac gcgcccgccg ctggttacca cctgctcgtc atccgccagt
    27361 tttgtgcccg cgtggaacac tttgccgtca gccacttctt ccagcggcag gccgtggatc
    27421 acatcgccgg tgcggtaatc gcccggatat ccacccgcag ccatcaccac gctgagagaa
    27481 gcacgttcat cccactctga cgttttctcg tccagtttgc cttcacaggc cgccaggcag
    27541 agctcaacca gatcggactt catgcgcagc ataatcggct gggtttccgg atcgccaaag
    27601 cggcagttaa attcaataac cttcggattg ccctgtttgt cgatcatcag gcccgcatag
    27661 agaaaaccgg tgtaggtgtt gccttccgcc gccatgcctt tcacggttgg ccagatgata
    27721 cgttccatgg tgcgctgatg aacttcatcg gttactaccg gcgcggggga gtaagcgccc
    27781 atcccgccgg tgtttggtcc gttatcttta tcgcctacgc gtttgtgatc ctggctggta
    27841 gccatcggca gcacatgctc gccgtccacc atcacgataa agctcgcttc ttcgccatcg
    27901 aggaactctt cgataacgat gcgatgaccc gcgtcgccaa aagcgttgcc cgccagcata
    27961 tcgtgaacag ccgcttccgc ttcttccagc gtcatcgcca cgataacgcc tttcccggca
    28021 gccagaccgt ccgctttaat gacgattggc gcgcctttct cacgcagata caccagcgca
    28081 ggttctacct cggtgaagtt ctggtattcc gccgtaggga tcttatggcg ggccaggaaa
    28141 tctttggtaa acgcttttga gccttccagt tgggccgcac ctgcggttgg gccgaagatt
    28201 ttcagcccgg cggcgcggaa ggtatcgacc acgcctttca ccagcggcgc ttccgggccg
    28261 acgatggtca gatcaatctt ttcgttttgt gcgaaatcca acagcgccgg gatatcggtc
    28321 acgccgatag caacgttttg cagcgcgggt tccagcgcag tgcctgcatt acccggagca
    28381 gcaaaaacag tctcaaccag cggcgactgg gccgctttcc aggccagcgc gtgctcgcgc
    28441 ccgccgttac caatcactaa tactttcatc tattgctcca ttaatggcgg aagtggcgca
    28501 tgtcggtgaa gagcatcgca ataccgtgct cgtcggcggc ggcaatcact tcgtcatcac
    28561 ggatggaacc gccaggctgg attacgcagg tcacgcccgc agcggcggcg gcatcaatac
    28621 cgtcgcggaa cgggaagaac gcgtcagaag ccatcgagga acctttcact tccaggcctt
    28681 catcggcagc tttaataccg gcgattttcg cggagtacac gcggctcatc tggcccgcgc
    28741 caataccgat ggtcatattg tttttggcat agacgatagc attggatttc acgaatttcg
    28801 ccactttcca gcagaacagc gcatcacgca gttcctgttc ggtcggctga cgttgggtga
    28861 cgacgcgcag ttcttccgca ccgaccatcc ccaggtcacg atcctgaacc agcagaccgc
    28921 cgttcacgcg tttgaaatca agacccggaa cacgctcgcc ccactgaccg caggtcagaa
    28981 cgcgtacgtt ctgcttggcg gcggtgattt tcagggcttc ttcgctggcg gaaggcgcaa
    29041 taatcacttc gacaaactga cgagaaatga tggcctgtgc ggtttccgca tccagctcgc
    29101 ggttaaaggc aatgatgccg ccgaatgcgg aggttgggtc ggttttgtac gcgcgatcgt
    29161 aagcatcaag aatggaattg ccgatagcta caccgcaagg gttggcgtgc ttcacaatca
    29221 cacatgccgg ctcggcgaac tctttcacgc actccagcgc cgcatcggta tcggcgatgt
    29281 tgttataaga gagggcttta ccctgaacct gggttgcggt agcaacggag gcttctttca
    29341 cattctcttc tatatagaag gcagcctgct ggtggctgtt ctcgccgtaa cgcatatcct
    29401 gcttcttaat gaagttcagg ttcagcgtgc gtgggaagcg accggcggct tctttgcttt
    29461 caccgtggta cgccggaacc atgctgccga agtagttggc aatcatgctg tcgtaggcgg
    29521 cagtgtgttc gaaggctttg atggcgaggt cgaaacgggt tgcaagcgtc agcgatcctt
    29581 cgttgtcatc catctcttta ataatggcgt cgtagtcgct gctcttcacc acgattgcga
    29641 catctttatg gttcttagcg gcggagcgca ccatggtcgg gccgccgata tcgatgttct
    29701 caaccgcatc ttccagcgag cagccttcac gagcaacagt ctgggcgaac ggatacaggt
    29761 taacaaccac catatcgata ggctggatct gatgttcttc cataatggca tcgtcctggc
    29821 cgcgacggcc cagaatgcca ccatgtactt tcggatgcag ggtcttcacg cgtccatcca
    29881 tcatctccgg gaaaccggtg taatcggaaa cttcggttac cggcagacct ttttctgcta
    29941 acagacgggc agtgccccct gtagacagca gctccacacc gcgtgcggaa agtgcctggg
    30001 cgaattcgac gataccggct ttgtcagaaa cactgagcag agcgcggcgg actggacgac
    30061 gttgttgcat ggtaaatccc ctgggtttga ctattacaga gagcgttagc tgaatttttc
    30121 gcgaaaaact cagctaacgc ccctaacggg gcatccttat ttttcgcccg cattgtaacg
    30181 aaaacgtttg cgcaacgctc gcgaattttt ctctttcaat ggtgatcaca attttgacag
    30241 tggttaccgt gggaaaaata cagaaattac attgatgatt gtggataact ctgtgcgtaa
    30301 aaaggtataa agcggggttt tgctggggaa tgcagcagtc agtcattttt ctgcaatttt
    30361 tctattgcgg cctgcggaga actccctata atgcgccttc atcgacacgg cggatgtgaa
    30421 tcacttcaca caaacagccg gttcggttga aga
//
LOCUS       DAZPAB010000027        30223 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZPAB010000027 DAZPAB010000000
VERSION     DAZPAB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30223
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.027"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="ACHUPG_001104"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="ACHUPG_001104"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR0860806.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="ACHUPG_001105"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="ACHUPG_001105"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR0860807.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="ACHUPG_001106"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="ACHUPG_001106"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR0860808.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="ACHUPG_001107"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="ACHUPG_001107"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR0860809.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="ACHUPG_001108"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="ACHUPG_001108"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR0860810.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="ACHUPG_001109"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="ACHUPG_001109"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR0860811.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="ACHUPG_001110"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="ACHUPG_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR0860812.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="ACHUPG_001111"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="ACHUPG_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR0860813.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="ACHUPG_001112"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="ACHUPG_001112"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR0860814.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="ACHUPG_001113"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="ACHUPG_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR0860815.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="ACHUPG_001114"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="ACHUPG_001114"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR0860816.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="ACHUPG_001115"
     CDS             complement(15665..16702)
                     /locus_tag="ACHUPG_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR0860817.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="ACHUPG_001116"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="ACHUPG_001116"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR0860818.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="ACHUPG_001117"
     CDS             complement(17582..19324)
                     /gene="ggt"
                     /locus_tag="ACHUPG_001117"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR0860819.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19444..19884
                     /locus_tag="ACHUPG_001118"
     CDS             19444..19884
                     /locus_tag="ACHUPG_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR0860820.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNSDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="ACHUPG_001119"
     CDS             complement(19871..20614)
                     /gene="ugpQ"
                     /locus_tag="ACHUPG_001119"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR0860821.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20611..21544)
                     /locus_tag="ACHUPG_001120"
                     /pseudo
     CDS             complement(20611..21544)
                     /locus_tag="ACHUPG_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="ACHUPG_001121"
     CDS             complement(21603..22448)
                     /gene="ugpE"
                     /locus_tag="ACHUPG_001121"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR0860822.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="ACHUPG_001122"
     CDS             complement(22445..23332)
                     /gene="ugpA"
                     /locus_tag="ACHUPG_001122"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR0860823.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="ACHUPG_001123"
     CDS             complement(23430..24746)
                     /gene="ugpB"
                     /locus_tag="ACHUPG_001123"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFR0860824.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25143..25856)
                     /gene="livF"
                     /locus_tag="ACHUPG_001124"
     CDS             complement(25143..25856)
                     /gene="livF"
                     /locus_tag="ACHUPG_001124"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR0860825.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25858..26625)
                     /gene="livG"
                     /locus_tag="ACHUPG_001125"
     CDS             complement(25858..26625)
                     /gene="livG"
                     /locus_tag="ACHUPG_001125"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR0860826.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26622..27899)
                     /gene="livM"
                     /locus_tag="ACHUPG_001126"
     CDS             complement(26622..27899)
                     /gene="livM"
                     /locus_tag="ACHUPG_001126"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR0860827.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27896..28822)
                     /gene="livH"
                     /locus_tag="ACHUPG_001127"
     CDS             complement(27896..28822)
                     /gene="livH"
                     /locus_tag="ACHUPG_001127"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR0860828.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28870..29979)
                     /gene="livK"
                     /locus_tag="ACHUPG_001128"
     CDS             complement(28870..29979)
                     /gene="livK"
                     /locus_tag="ACHUPG_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR0860829.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
BASE COUNT         7310 a         8424 c         7626 g         6863 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcaca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctctaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagctgcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt caacgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctacttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattgc cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgatacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cacccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgtcgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc
    19261 taaaacaact tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta
    19321 tcatcgttat tctccagaga ttaaggggca accccagcta agcctggtat ataactctga
    19381 attaatcatc gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac
    19441 gcgatgaaac gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc
    19501 attaatactc tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg
    19561 caaacccaga tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa
    19621 acgcaaactc agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg
    19681 gtgttgcagt cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc
    19741 aacggcggga tgttaaacag caaccgtaat ccggatagtt cgttgaatca gcagcatatg
    19801 ctgccggaga ggagaaacag cgacatgctg aatcagccca gcacgccgca gcctgacatt
    19861 ccgttgaaaa ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga
    19921 tgcaatccac gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa
    19981 ccagaatccg taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat
    20041 gattgagatg aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc
    20101 actcatcaag caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag
    20161 catcaatctc aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg
    20221 ccagcgccac cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat
    20281 tcgccatcat cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct
    20341 cacctttaaa cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt
    20401 tcagttcacc cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca
    20461 ggaagatctc gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt
    20521 ttgccccgac gtcgattgcc gccagggtgt tttccggggc cagcttaccg ccgccacgat
    20581 gagcgacgat gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa
    20641 aagatgcagc tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc
    20701 ctgatgcgcc agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc
    20761 gaatgccgag ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca
    20821 cgataccgcc gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt
    20881 tcatcgccgg gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt
    20941 ccaccggcgt gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga
    21001 gcgtcatcgc ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt
    21061 gcagctgttg cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg
    21121 gctcatcaaa caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct
    21181 ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa
    21241 tgcgcgccgc ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca
    21301 gcccccacgc catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca
    21361 ccatcgcaat cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga
    21421 tatcgccttc ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc
    21481 agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt
    21541 taatgaccta ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca
    21601 tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc
    21661 actactggtg ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg
    21721 ccttcgccag tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg
    21781 gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac
    21841 agcgccgcca gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt
    21901 gacgcaccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg
    21961 cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg
    22021 tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc
    22081 agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag
    22141 acaatggcaa atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc
    22201 atcacaaagc tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc
    22261 acccagatgt tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg
    22321 gcgtagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg
    22381 atcaccgcga tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc
    22441 tcaatcattg gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga
    22501 tgacgaggaa catcagcacc atcgactgtg cggcagacga agccagatcc agtccggtaa
    22561 aaccttcgcg gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg
    22621 tggcggcgtc gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca
    22681 ggaagaaact caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc
    22741 ctgcaccgtc gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat
    22801 agaagaacag aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca
    22861 tcgcctgacc gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg
    22921 tgatcagccc gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag
    22981 ccacggcata cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact
    23041 ccaccagcgc cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga
    23101 attttatcgt cgtccagaag gagtcgagat aatagctgtc atgaaacagc gtgacgaagt
    23161 tatccagacc gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc
    23221 acaacgcttc gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga
    23281 ccagcagata aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta
    23341 attcctgaac tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga
    23401 taagcgaaac gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc
    23461 agtaactggt tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg
    23521 gtccacacgc tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga
    23581 cgaagccctt tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc
    23641 gcacctgggt ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta
    23701 attggcagat aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag
    23761 tcgagaaact tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg
    23821 ctggctccgc cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc
    23881 atacctacgc cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag
    23941 gcggtggtca tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg
    24001 acgtagctga agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc
    24061 tccggcttgt tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca
    24121 aacggcagac cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg
    24181 gcgtagccgc acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc
    24241 caggttttcg gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa
    24301 tagagaacgg aagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg
    24361 gagtagtaac cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct
    24421 ttaaacacgt catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact
    24481 tcataaacct gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt
    24541 aaattctgtt cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg
    24601 ttaaaacgtt gggccagaga atccacctct ttacccagtt ccccttccat agaatgccag
    24661 aacggaatgg tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct
    24721 gaagctgtat aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat
    24781 tcacgcgttt tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt
    24841 ttgttacatt tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt
    24901 gagggaaaat gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc
    24961 tacagattac tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga
    25021 tcgtgcaaat tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg
    25081 caattttgca tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt
    25141 tattacccgc ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta
    25201 tcggagagca ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc
    25261 tggttggcgt tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg
    25321 gtgtcgaaaa tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac
    25381 aacaaacgcg gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg
    25441 gacatggtgc ccgcccgctg aacacggcgc tcatgcagac gtggaaacag ttcatacacc
    25501 cactttatgc gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc
    25561 tcttccaccg tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg
    25621 cgcatgattt tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg
    25681 ctggtggcgc gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc
    25741 gcgccaatga gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc
    25801 tggattttgc cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta
    25861 tgcctcacct aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc
    25921 gtttgccagt ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag
    25981 cttcatatcg tgttcaatca acaaaatagt ggtgttgtga tgattgcgca gttcggcaat
    26041 cagctcatcc agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat
    26101 taaaatctcc ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata
    26161 ggccaggtta ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt
    26221 ggcggcgcgg tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc
    26281 agagaacagc ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt
    26341 catttcacgg aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg
    26401 ctgccccggt aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt
    26461 gtagaaaccg gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa
    26521 cgagacgatt tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc
    26581 gaagcgcatc atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc
    26641 gctgcgccgt ttttcagctt cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc
    26701 cagatcatca tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca
    26761 cgcatcaatt cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag
    26821 cccataccgc cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat
    26881 tccgggctga caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg
    26941 gcgctgatgg taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag
    27001 gcgatctcgt cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg
    27061 ttaatgacaa acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg
    27121 cgatcggacg gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca
    27181 cgagcggtac ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg
    27241 ttcgggccgc cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa
    27301 ccaagggtaa cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg
    27361 aaacccgccg ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg
    27421 taatagtgat tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc
    27481 agcaccagca gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag
    27541 atcatggtca gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc
    27601 acaagcacca gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg
    27661 ggcagaataa acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa
    27721 agctggaaga aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa
    27781 gccgtgtcga ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc
    27841 gccaggacaa agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata
    27901 ctttctctac ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca
    27961 gcagggcgaa tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct
    28021 ccgcaatccc caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga
    28081 gcaccgcagc ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc
    28141 agtagaactg accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg
    28201 tcaaagcaat cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg
    28261 cgcaggcacg acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgccagca
    28321 tggcgaggaa ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt
    28381 tttcgctatg gcccaccacc cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg
    28441 aaccttcggt caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa
    28501 gcgcaatcag gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc
    28561 cgtaggcgct ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg
    28621 tatcaatgcc catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca
    28681 tataaacctc gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc
    28741 cgatggctat cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga
    28801 aatacaaaaa ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg
    28861 gtgggatgat cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa
    28921 atcaaatccc ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc
    28981 accgttagct tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag
    29041 ggcagttgcc agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc
    29101 tttcttgtct gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg
    29161 ttttggcata gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc
    29221 gttaccgaca ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg
    29281 cagcatctgc cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt
    29341 tttcaggcgg gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa
    29401 gacgacgttg gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc
    29461 atactgttgt ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta
    29521 ttttgccgcc gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata
    29581 accgcgttgg gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc
    29641 ttcgtagata tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt
    29701 aatgccgtcg ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc
    29761 gtcatattcc acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt
    29821 aatcgcctga cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat
    29881 cgcgccgaca acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc
    29941 aatcatccct gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg
    30001 atgttgtgtt gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca
    30061 ctgtttttgc ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa
    30121 tccctatttt tcagtcgatt aagaacagat aatattctga atttattgat agataaacag
    30181 aaaaaagtgc ctttgtcagc ataaaataac ggcacaaagg gcg
//
LOCUS       DAZPAB010000028        29946 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZPAB010000028 DAZPAB010000000
VERSION     DAZPAB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29946
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.028"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            128..484
                     /locus_tag="ACHUPG_001129"
     CDS             128..484
                     /locus_tag="ACHUPG_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR0860830.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            488..1615
                     /gene="dapE"
                     /locus_tag="ACHUPG_001130"
     CDS             488..1615
                     /gene="dapE"
                     /locus_tag="ACHUPG_001130"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR0860831.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            1643..1843
                     /locus_tag="ACHUPG_001131"
     CDS             1643..1843
                     /locus_tag="ACHUPG_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR0860832.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(1953..2651)
                     /gene="ypfH"
                     /locus_tag="ACHUPG_001132"
     CDS             complement(1953..2651)
                     /gene="ypfH"
                     /locus_tag="ACHUPG_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0860833.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(2725..4740)
                     /gene="tmcA"
                     /locus_tag="ACHUPG_001133"
     CDS             complement(2725..4740)
                     /gene="tmcA"
                     /locus_tag="ACHUPG_001133"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR0860834.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(4755..5618)
                     /locus_tag="ACHUPG_001134"
     CDS             complement(4755..5618)
                     /locus_tag="ACHUPG_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR0860835.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(5786..6499)
                     /gene="purC"
                     /locus_tag="ACHUPG_001135"
     CDS             complement(5786..6499)
                     /gene="purC"
                     /locus_tag="ACHUPG_001135"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR0860836.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(6712..7746)
                     /gene="bamC"
                     /locus_tag="ACHUPG_001136"
     CDS             complement(6712..7746)
                     /gene="bamC"
                     /locus_tag="ACHUPG_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR0860837.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(7763..8641)
                     /gene="dapA"
                     /locus_tag="ACHUPG_001137"
     CDS             complement(7763..8641)
                     /gene="dapA"
                     /locus_tag="ACHUPG_001137"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR0860838.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            8787..9359
                     /locus_tag="ACHUPG_001138"
     CDS             8787..9359
                     /locus_tag="ACHUPG_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR0860839.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            9359..9829
                     /gene="bcp"
                     /locus_tag="ACHUPG_001139"
     CDS             9359..9829
                     /gene="bcp"
                     /locus_tag="ACHUPG_001139"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR0860840.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            10082..10699
                     /gene="hyfA"
                     /locus_tag="ACHUPG_001140"
     CDS             10082..10699
                     /gene="hyfA"
                     /locus_tag="ACHUPG_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR0860841.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            10699..12716
                     /gene="hyfB"
                     /locus_tag="ACHUPG_001141"
                     /pseudo
     CDS             10699..12716
                     /gene="hyfB"
                     /locus_tag="ACHUPG_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            12727..13674
                     /gene="hyfC"
                     /locus_tag="ACHUPG_001142"
     CDS             12727..13674
                     /gene="hyfC"
                     /locus_tag="ACHUPG_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR0860842.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            13691..15130
                     /gene="hyfD"
                     /locus_tag="ACHUPG_001143"
     CDS             13691..15130
                     /gene="hyfD"
                     /locus_tag="ACHUPG_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFR0860843.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            15142..15792
                     /gene="hyfE"
                     /locus_tag="ACHUPG_001144"
     CDS             15142..15792
                     /gene="hyfE"
                     /locus_tag="ACHUPG_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR0860844.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            15797..17389
                     /gene="hyfF"
                     /locus_tag="ACHUPG_001145"
     CDS             15797..17389
                     /gene="hyfF"
                     /locus_tag="ACHUPG_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR0860845.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMA
                     KPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQ
                     RSTWHDFLPSGTASVSEKHSER"
     gene            17379..19093
                     /gene="hyfG"
                     /locus_tag="ACHUPG_001146"
                     /pseudo
     CDS             17379..19093
                     /gene="hyfG"
                     /locus_tag="ACHUPG_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            19103..19648
                     /gene="hyfH"
                     /locus_tag="ACHUPG_001147"
     CDS             19103..19648
                     /gene="hyfH"
                     /locus_tag="ACHUPG_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR0860846.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            19645..20403
                     /gene="hyfI"
                     /locus_tag="ACHUPG_001148"
     CDS             19645..20403
                     /gene="hyfI"
                     /locus_tag="ACHUPG_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR0860847.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            20396..20809
                     /gene="hyfJ"
                     /locus_tag="ACHUPG_001149"
     CDS             20396..20809
                     /gene="hyfJ"
                     /locus_tag="ACHUPG_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR0860848.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            20839..22851
                     /gene="hyfR"
                     /locus_tag="ACHUPG_001150"
                     /pseudo
     CDS             20839..22851
                     /gene="hyfR"
                     /locus_tag="ACHUPG_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            22873..23745
                     /gene="focB"
                     /locus_tag="ACHUPG_001151"
                     /pseudo
     CDS             22873..23745
                     /gene="focB"
                     /locus_tag="ACHUPG_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
     gene            complement(23839..24900)
                     /locus_tag="ACHUPG_001152"
     CDS             complement(23839..24900)
                     /locus_tag="ACHUPG_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR0860849.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            25113..26576
                     /gene="bepA"
                     /locus_tag="ACHUPG_001153"
     CDS             25113..26576
                     /gene="bepA"
                     /locus_tag="ACHUPG_001153"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR0860850.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            26597..26956
                     /gene="arsC"
                     /locus_tag="ACHUPG_001154"
     CDS             26597..26956
                     /gene="arsC"
                     /locus_tag="ACHUPG_001154"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR0860851.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(27089..27790)
                     /locus_tag="ACHUPG_001155"
     CDS             complement(27089..27790)
                     /locus_tag="ACHUPG_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR0860852.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(27885..29174)
                     /gene="uraA"
                     /locus_tag="ACHUPG_001156"
     CDS             complement(27885..29174)
                     /gene="uraA"
                     /locus_tag="ACHUPG_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR0860853.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(29260..29886)
                     /gene="upp"
                     /locus_tag="ACHUPG_001157"
     CDS             complement(29260..29886)
                     /gene="upp"
                     /locus_tag="ACHUPG_001157"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR0860854.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
BASE COUNT         6803 a         8028 c         7822 g         7293 t
ORIGIN      
        1 taatattatt taatagttgt taatttgaat acttcgataa tgttatattt tctgataatc
       61 atttgcaggc aaaatgtttt cacccttaaa tgagtattta ttctcataaa tcgaaaaagg
      121 attcattatg gttacacttt acggcatcaa aaattgtgac accattaaaa aggctcgccg
      181 ttggctggaa gccaataaca tcgactatcg ttttcatgat taccgcgtcg atgggctgga
      241 cagcgaatta ttgaacggtt ttatcaacga attaggctgg gaagcgttac tcaacacccg
      301 tggtacaacc tggcgtaaac tggacgaaac tacccgcaat aaaatcaccg atgcggcctc
      361 tgcggcggca ttaatgactg aaatgcctgc aattatcaaa cgtccattgc tctgcgcgcc
      421 tggtaagcct atgctgctgg gtttcagtga ttccagttat cagcaatttt tccatgaggt
      481 gtagtctatg tcgtgcccgg ttattgagct gacacaacag cttattcgcc gcccttccct
      541 gagtcctgat gacgcaggat gtcaggcttt gttgattgaa cgtttgcagg cgatcggttt
      601 taccgttgaa cgcatggatt ttgccgatac gcagaatttt tgggcatggc gtgggcaagg
      661 tgaaacgtta gcctttgccg ggcataccga cgtggtgccg cctggcgacg ccgatcgttg
      721 gatcaatccg ccgtttgaac ccaccattcg tgacggcatg ttattcgggc gcggtgcggc
      781 agatatgaaa ggctcgctgg cggcgatggt ggtagcagca gaacgttttg tcgcacaaca
      841 tcccaaccat acggggcgac tggcatttct gatcacttct gatgaagaag ccagtgccca
      901 caacggtacg gtaaaagtcg tcgaggcgtt aatggcacgt aatgagcgtc tcgattactg
      961 cctggtcggc gaaccgtcga gtatcgaagt ggtaggtgat gtggtgaaaa atggtcgtcg
     1021 cggatcgtta acctgcaacc tcaccattca tggcgttcag gggcatgttg cctacccaca
     1081 tctggctgac aatccggtac atcgcgcagc acctttcctt aatgaattag tggctattga
     1141 gtgggatcag ggcaatgaat tcttcccggc gaccagtatg cagattgcca atattcaggc
     1201 gggaacgggc agtaacaatg ttattccggg tgaactgttt gtgcagttta acttccgctt
     1261 cagcaccgaa ctgactgatg agatgatcaa agcgcaggta cttgccctgc ttgaaaaaca
     1321 tcaactgcgc tatacggtgg attggtggct ttccgggcag ccatttttga ccgcgcgcgg
     1381 taaactggtg gatgcggtcg ttaacgcggt tgagcactat aatgaaatta aaccgcagct
     1441 actgaccaca ggcggaacgt ccgacgggcg ctttattgct cgcatggggg cgcaggtggt
     1501 ggaactcggg ccggtcaatg ccactattca taaaattaat gaatgtgtga acgctgccga
     1561 cctgcagcta cttgcccgta tgtatcaacg tatcatggaa cagctcgtcg cgtgatgagt
     1621 ggttctgcaa gaggaaataa gcatggactg gctggctaaa tattggtgga ttctggtgat
     1681 tgtctttttg gtaggcgtcc tgctgaacgt gattaaagat ctcaagcgcg tcgaccataa
     1741 gaaatttctc gccaacaagc cggagcttcc cccgcatcgt gatttcaacg ataagtggga
     1801 cgatgacgac gactggccga aaaaggatca accgaagaag taagctgagt gctcaaccgt
     1861 aggccggata aggcgttaac gccgcatccg gcaatggtga acgatccctg atgcgacgct
     1921 cacgcgtctt atcaggccag gtaatctgtc ttttacatca tttcaatcac atcatcatcg
     1981 ccaggcttac cgccacttag cgcttggtcg aaataatgtt tcggaatggt ataacgcaag
     2041 tgatccaggg caaattgcat actgcgattg tcgatcgcat ggcctaaatc ctccacgata
     2101 tccagcgtca cgtcaccacc ggcactgatt aacgcttcct gcgccgcaac tgcatgcgcc
     2161 agatcgataa ccggatcttc accaccatga atcaagtgaa ttgtcgtggc tgtagacgcg
     2221 gtctccggca ggctcgcata gcgtccgtta aaggcaatga cgcgcgaagc aaggcccggt
     2281 tcggctttaa tgctctccag cgccataatc gcgccttgtg agaaaccaat tagcgccgtg
     2341 gcgttagccc ctactccgct ctgtttctgc cagtagcgta ccgtttcaat aaacgtcggc
     2401 atgatggcat ccacacgcgc ctggcgatta tcttccgtaa taccctgcac tgaaaaccac
     2461 tgacgccccg ccggattacc gctcggctcc gcgccaccga cactcaccac cagcgcatca
     2521 ggaaacagcg gtgcaaacca gctgcctatt tcccccattg ccaccgggtt atccccgaca
     2581 ccgtggaaaa gcagtaacaa ctgttgtgca ggtttatccg ggctttgaac aacaaaatgg
     2641 tcatgtttca tggcgaactc cttaactgat gtcattaatt ttacgccgct gactcacaat
     2701 gaccatggga gaaaattgaa ggagtcagtg aaaaaattgc cattgcgtta tgcgatcgcg
     2761 cagacgctca gtgcgaacct catttagtgc gaacagcgcc tgcgcggcct cttcccgctg
     2821 gcgaaccagt aacatcttgc ggcctgaaag tttaagtgtg gtacataact gcgcgtcact
     2881 ggcgtttttc tgtaaacgcc cacgcagcgc cggtaatgcc agttcactgg tttgtagcag
     2941 acgcaataag caacctaacg atgttaatag cggacgatga gcgaaagcaa aaccggccag
     3001 ttcaagccag tcgtcgtcag aaaggatggc atcgtttagt ggatcaacag gaagcatttc
     3061 gccattccac tgcgcgagag cttgcgcatc gcgacgtaaa cggtaatgct cacgttcagc
     3121 cagctgttta cccgcatcac tcatcggtaa cagcgccatc gccgtatagc aaccgctgct
     3181 ggcttcccga tgattaccca tccgcaccag cacaaaaccg cagcgttgcc agaaacgcca
     3241 taactcccca gtgtaaccaa aactcaccga aagatagtcg aggtcatgcg tatattgcaa
     3301 agctccagca ataagttgtc gccctgtgcc ttcccgctga cgagccggat gaactgctat
     3361 ccggctaacc cgccttccgc gcaatgtcgc cgccagtgga ttgctgccgt gcgccgccag
     3421 cgactgggcc accagattac cccgcgggcg acgaaaacct gcccataccg cctgactgag
     3481 ttcttgagat aatccgccct catccaccag ccacaacgcc ccggcaatct cgttttcgcc
     3541 agccgcctgt aaaaaatgtt gccctggtgc atccatcatc cggcgtaaat ccagcggcga
     3601 ggtccggtag tgcgcaccag acaataactg ataaaccttt aacggcgttt ctggctcgct
     3661 tcgccataac gtttgttcaa atgcggaaat gacgatattg ccttgtggtg tatgggtgaa
     3721 gttttcatcg ttaaaaacca gtgcctcact aaccattttt tccagcgggc atccctgtgc
     3781 ccagcggatc ggctgttgca gttcaaaacg gtgtaaatgc ggaaagcgag cgcaaaattt
     3841 cagcaaaaaa ccacgtccgg taccttcgta gccctgcacc gtagtggtta acaacgttcg
     3901 aggaaaacgc gataccagtt gatgcaacaa cggcgcaggt atggctgcgg cttcgtcgac
     3961 caccagccag tcggcttgct catcgctggc taacaaggca tccggtgcaa taaagcgaaa
     4021 cttctcgccc gcaaattgtg ccagtacatc cgttgccgct tttgcgggcg cggtgacaat
     4081 cgcactgccc gcaatacgag aaatgagttg ccctgccaac gccgacttac cgcgcccacg
     4141 cgcagccgtt accgctgcca cgcccggcgg catggtcagt agctgctgta agagttgctg
     4201 ttgttctggt tgtggtgcgc cggtcgcggg gtgccagtca gtacggggag taaaatgcgc
     4261 caacgagaac ggctggtttt gccgccagag gatagcgtcg ttatccgccg taagtacgcg
     4321 tttgagatgc tggacaaaat gcggcgtcgc aatagggtca gggcaatcac tccagcgcag
     4381 cgagtcggca tcaggttggt tttcccactc ttcccataca gggagtaaca aaaccagcca
     4441 gcttcccgct ttcaacgttc cgctaagtgc tgcaaaggca gcggcatcaa agccgtggcg
     4501 ggcgtcgaat accgcatgcc ggaactcgcg cccaagtaaa gtttgtagcg ccgagggtga
     4561 acagtggttt tcagcatcta gctgcggcga aatccacagc cagtcgccag gtaaggcatc
     4621 acgcaactta agagcatgat caaaacacca accctcttcc ccgctcaaca ccagcaagcg
     4681 gcagatccct tcacgtttca tttgcgctgt taatgtgtaa agcgcagtca gttcagccat
     4741 ccctgccccg gaaattaaat gcttttacca aaagtattgc attgtgccgg atcgccgctg
     4801 tcgaaaccac gtttaaacca gctgtagcgt tgctgagaag tgccatgtgt gaaactgtct
     4861 ggtactactc gcccctgact ttgctgttgt aaacggtcat cgccgatggc ctgcgccgca
     4921 ttcagcgcct cttccagatc gccagtttcc agaacgcctt gctgctgcat actatgcccc
     4981 cagacaccgg caaaacagtc ggcctggagt tccatacgca cagataagcg gtttacttcc
     5041 gcctgcgtcg cgttttgttg cagttgacga actttcggct cgatgcctaa cagtttctgc
     5101 acatgatgac cgacttcatg ggcgataacg tacccctggg caaaatcgcc atccgcgcca
     5161 agtttgtctt tcatgtcatc atagaaggag agatcgatat aaactgtgcc atccgccggg
     5221 caatagaacg gccccataat ggactggccc gccccgcagc cggtgcgcgt cattccacgg
     5281 tacatgacca gcttcggttg ctgataggtc ttacccatct tttcgaactg ttgtccccag
     5341 gtgtcttccg tggttgcctg aatcaccgag gtgaattttg cggcttcgtc ttcattaggg
     5401 ctaattgacc gcgttgattg ttgttgagaa accggctgcc cggtcatcaa cccggttaaa
     5461 tcaacgccat agtagcctgc aaccagcacg actatcagta aaatcagccc gcctttaccg
     5521 ctcggcagac ggaaaccggg accgcccatt gacggaccgc cagagctgtt gcgcctgtct
     5581 tcaacattgt cactttcacg tcgcccttgc caacgcatag atacctcaac aatatattca
     5641 ttatagaaat gatcgtaggt ggttcagagg aagattacca caggaaagcg ggatgaaatg
     5701 gcggaggaat gagcgttcaa tgcatatatt ccaagagcgg ttgtcttgcc gtgccagctg
     5761 cacggcaaga tgatgaacag aaaaatcagt ccagctgtac acccaggcgg cgggcgacgg
     5821 cttcataggc ttcgatcagg ccaccgaggc tctggcggaa acggtctttg tccattttct
     5881 ccagtgtttc tttgtcccac aggcggctgc cgtccgggga gaactcatcg cccagtacca
     5941 cttcaccttt gtacagacca aactccagct tgaagtcgac cagaatcaga ccagcatcat
     6001 cgaacagttt tttcagcacg tcgttcgctt tgtaggtcag ctctttcata cgcgccaggt
     6061 tctctttgct cacccagcca aaggtttcgc agtaagattc gttgaccatc ggatcgtgca
     6121 tggcatcgtt tttcaggaac agatcgaaca gcggcgggtt cagctcaata ccttcttcga
     6181 ttccaagacg tttcactaga gagccagcag cacggttacg cacgacacac tcaaccggca
     6241 ccatatccag ctttttcacc agacattcgg tatcggagag cagacgctcc atttgagtcg
     6301 ggatacccgc ttcagccagt ttgctcataa tgaagtagtt gaacttgttg ttcaccatac
     6361 ctttgcgatc aaactgctca atgcgcgcgc catcccctgc tgacgtatca ttgcggaatt
     6421 cgagcaccaa caggtccggg ttttccgtgc tgtatacggt tttcgcttta ccacgataca
     6481 actcagcttg cttttgcatc tttatcactc ctgggtgtga attaacgttt taaaatcttt
     6541 tgctgtctgg tgtgccggat gttttgtcgg atgcggcgtg aacgccttat ccgaactacg
     6601 tccgacaaac aaatttcgtg cgaattaccg ctaaaatcgc gttttcctgc cgacgcacac
     6661 gtttgcgtat catatcagaa aaaagggccg gatgattcca gccctgtatt tttacttgct
     6721 aaacgcagcc tggaagacag ctaccagcgc gtcgttctga ctctgagtca gagtatgacc
     6781 tttcggatcg atgaactgta ggctgctgcg gttatctaaa tcgccaacct gcagtttata
     6841 gtcaccggat gccaggcctg gatcgctcgc gcccagttcc tgccagtcgc tgtcagacag
     6901 cggcttataa gttacggcca tgttgccctg cgaacgggtg ctgtcggtca ctttcatgcc
     6961 cactttttcc agcgccgctg gcagacgttg ccaaaccaca ttgaacggcc cgcgtacgac
     7021 cagcattggt aaaccggtgt catcagctgc actttgtacg tccatagtgg tggaggcacg
     7081 attttgcgca gcgttcgcgg cgtcagtggc agatttatcc agaccggcgg aaataacgtt
     7141 catcatctcc gtgctgtaac gctgcatgga agccgcgtct gcaaccggtt tgcccgcctg
     7201 ttccaggttc agcagtttaa ccgtaaccgc ctgctgataa ccctgcggct taacagagat
     7261 ttgataacga ccacgatact gctcgtcttc gtccagacgg ttccattgta cccaatcggt
     7321 ggtcagtgtc tgaccagcat catcacgttg ggtgatggtg tagtttttcg cctgcagcac
     7381 gctaaccacc tgcggccaca gagtattgcc acgaccattt tccaccagca atgaagcggt
     7441 atcgcccgtg aactgggtac gcgcgccaga aaccagtgcc aacggctggg ctggtggacg
     7501 aatgtccagc gccttaccga cagcaccact accgttggtc accgggattg cataatcacc
     7561 ggaggtcacc ggcaaaatca ttccagccgg ggcatgaagc tccgcaagcg gtgccgcttc
     7621 caggtaggct tcatcaccac tgacctgacg cttatagcgt gagtcagaac tacaggcagc
     7681 gagtaataaa acaagcgaaa cacccgcaac ctttgccagg cgcgactttt gaacagagta
     7741 agccatcaaa tctccctaaa ctttacagca aaccggcatg cttaagcgcc gctctgaccg
     7801 tctcacgacc actgtcggtg attggtgtca ttggcaggcg cagcgtatcg gtcgccacaa
     7861 gacccagttc cttacatgcc catttcaccg ggattggatt gggttcgaca aatagtttgt
     7921 tgtgtaatgg catcagacgc tgattaataa cgcgtgcctc ggcaaaatgc ccttctgctg
     7981 ccagtttgca catctgggcc atatcacgcg ctgcgacgtt agccgtaacg gaaataactc
     8041 catgaccgcc caattgcatg aagtccagcg cgctcgcatc atcgccgctc agcagaacaa
     8101 aatcatctga aaccagctct ttgatctggt ttacacgcgt taagttccct gttgcctctt
     8161 tgattccgat aatatttttt actttcgcca gacggcccac cgtttccggg agcagatcgc
     8221 agccagtacg ggacggcaca ttatacagaa tttgcggcag gtcagtatgc tcagcgatgg
     8281 ctttgaaatg ctgatacaaa ccttcttgcg acggacgatt gtagtaaggg gttaccgtca
     8341 ggcagccgac gataccactg tcattgaagc gctgcgtcag gctaatggct tccgcagtag
     8401 cgttagcgcc ggtcccggca attaccggaa tgcgcccatc agccagatcc agcgtcatca
     8461 tcaccacatc agcatgttcg tcatgattta aggtagcgga ctcgccagtg gtgccaacag
     8521 aaacgatcgc cgaagtaccg ctggcgacat gataatcaat cagttttttc aagctagccc
     8581 gacagacatt acctttttca tccatcggag taacaatcgc gacaatactt cccgtgaaca
     8641 tgggccatcc tctgtgcaaa caagtgtctc aatggtacgt ttggtatggc attaaaagca
     8701 agcagacaga accgttctga ttgttgtatg catgattttt ttatgctttc cttaagaaca
     8761 actcacccct taaaggaata accagtttga cactgtcatc gcaacattat ctggtgatca
     8821 ctgcgttggg tgccgatcgc cctggaattg tgaacaccat cacccgtcat gtcagtagtt
     8881 gcggctgtaa tattgaagac agtcgcctgg cgatgctggg agaagagttc acgtttatca
     8941 tgctgctttc cggttcatgg aatgccatca ctctgattga atcaacgtta ccgttgaaag
     9001 gtgccgaact ggatctttta atcgtgatga agcgcacgac ggcgcgtccg cgtccgccaa
     9061 tgccagcatc tgtctgggtt caggtcgatg ttgcagactc cccgcattta attgaacgct
     9121 tcacagcact tttcgacgcg catcatatga acattgcgga gctggtgtcg cgcacacaac
     9181 ctgctgaaaa tgaacgggct gcgcagttgc atattcagat aaccgcccac agccccgcat
     9241 ctgcggacgc agcaaatatt gagcaagcgt tcaaagccct atgtacagaa ctcaatgcac
     9301 aaggcagtat taacgtcgtc aattattccc aacatgatga acaggatgga gttaagtaat
     9361 gaatccactg aaagccggtg atatcgcacc gaaatttagc ttgccggatc aagacggaga
     9421 acaagttaat ttgaccgact tccagggaca gcgtgttctg gtttatttct acccgaaagc
     9481 catgaccccc ggctgtaccg tacaggcctg cggcttacgc gataacatgg atgagttgaa
     9541 aaaagcgggc gttgatgtgc tgggtatcag caccgataaa cccgaaaaac tctcccgttt
     9601 tgcggaaaaa gagctgctta actttacgct cctgtctgat gaggaccacc aggtgtgcga
     9661 acaattcggc gtctggggtg aaaagtcctt catgggcaaa acctacgatg gtattcatcg
     9721 catcagcttc ctgattgacg ctgatggcaa aatcgaacat gtctttgacg atttcaaaac
     9781 cagcaatcac cacgacgttg tgctgaactg gctgaaagaa cacgcctgat tactttgctc
     9841 cattccgtgc tggctgcgct tgcggccagc atacctcact tctcgtgatc aagatcacat
     9901 tctcgctttc ccctgcgaca cgggtgtcga atccattttt tgctgaacgt taatgaccat
     9961 catttttgta ccgttcagaa tccagttaat acataactta ttgaatagat tgagttaatc
    10021 agaatggcat cctttataca atatgaaatg caatgtttca tatcattttc aaggagccga
    10081 catgaaccgc tttgtggtgg ccgaaccact gtggtgtaca ggatgtaata cctgtctcgc
    10141 tgcctgttcg gacgtgcata aaacgcaagg tttacagcaa cacccgcgcc tggccctggc
    10201 gaagacgtca acaatcactg cccctgtcgt gtgtcatcac tgtgaggaag ccccttgcct
    10261 gcaggtctgc ccggtcaatg ccatctctca gagggatgat gcgatccaac tcaacgaaag
    10321 cctctgtatt ggctgcaagc tttgcgccgt ggtctgccca tttggcgcaa tcagcgcttc
    10381 aggaagccgt ccggtgaatg cccatgcgca atatgttttt caggctgaag gctcactcaa
    10441 agacggcgaa gaaaacgtgc cgccccaaca tgcgttgctg cgctgggaac cgggtgtcca
    10501 gaccgtcgcg gtgaaatgcg acctgtgtga tttcttgcca gaaggtccgg cctgcgttcg
    10561 cgcttgcccg aatcaggcgt tacggctgat caccgacgat agcctgcaac gtcagatgaa
    10621 agaaaaacag cgccttgccg caagctggtt tgccaatggc ggggagtctc ccctttccct
    10681 cactcaggag caacactaat ggatgccctg caattattaa cctggtcgct gatcctctat
    10741 ctgtttgcca gtctggcttc gctgttttta ctcggtctgg acagactggc tattaagctt
    10801 tccggcatca catcgctggt gggcggcgtg attggcatca tcagcggaat tacgcaatta
    10861 catgctggtg taactttagt cgcccatttt gccacccctt ttgactttgc cgatttaacc
    10921 ctgcgaatgg atagcctctc ggcatttatg gtgctagtta tctccttgct ggtggtggtt
    10981 tgttcgctct attcattgac ttatatgcgc gaatacgagg gcaaaggcgc ggcggcgatg
    11041 ggcttcttta tgaatctttt catcgcatcg atggttgccc tgctggtgat ggacaacgct
    11101 ttttggttca tcgtgctgtt tgaaatgatg tcgctgtctt cctggtttct ggtcattgcc
    11161 aggcaggata aaacgtcgat caacgctggc atgctctact tttttatcgc ccacgccgga
    11221 tcggtgctga ttatgatcgc cttcttgctg atggggcgcg aaagcggcag cctcgatttt
    11281 gccagtttcg cacgctttca ctttctccgg ggctggcgtc ggcggtgttt ctgctggcct
    11341 ttttcggttt tggcgcgaaa gccgggatga tgccgttgca cagctggttg ccgcgcgctc
    11401 accctgccgc accatcgcac gcttcggcgt tgatgtctgg cgtaatggtc aaaatcggta
    11461 ttttcggcat cctgaaagta gcgatggatc tgctggcgca aacgggtttg cctctgtggt
    11521 ggggcattct ggtgatggcg atcggcgcaa tctccgcgct cctgggcgtg ctatatgcgc
    11581 tggcggaaca ggatatcaaa cggctgctgg cctggagtac cgtcgaaaac gtcggcatta
    11641 ttttgctggc agtcggtgtg gcgatggacg gtctgtcact gcacgacccg ctgctcaccg
    11701 tggttggact gctcggcgca ctgtttcatc tgctcaacca tgcgctgttc aaagggctgc
    11761 tatttctcgg cgcggacgcg attatttcgc gtttgcatac ccacgacatg gaaaaaatgg
    11821 gggcactggc gaaacggatg ccgtggacag ccgcagcatg cctgattggt tgcctcgcga
    11881 tatcagccat tcctccgctg aatggtttta tcagcgaatg gtacacctgg cagtcgctgt
    11941 tctcactaag tcgtgtggaa gccgtagcgc tacaacttgc gggtcctatt gctatggtaa
    12001 tgctggcagt cactggtggg ctggcagtaa tgtgcttcgt caaaatgtac ggtattactt
    12061 tctgtggtgc gccgcgcagt acacacgctg aagaggcaca ggaagtgcca aatacgatga
    12121 tcgtcgccat gctactgctc gcggcactct gcgtattaat tgcgcttagt gccagttggc
    12181 tggcaccgaa gataatgcat attgcccatg cgtttaccaa tacccctccc gtcactgtcg
    12241 ccagcggaat agcacttgta cccggcacgt ttcatacacg ggtcactcct tcattactgt
    12301 tgctgttact actggcgatg cctttgctgc caggccttta ctggctgtgg tgtcgttcgc
    12361 gccgcgcagc gtttcgtcgc acaggagatg cctgggcatg cggctacggc tgggaaaacg
    12421 cgatggctcc gtcaggcaat ggcgtgatgc agccgctgcg tgtggtcttc tctgcgttat
    12481 ttcgtctacg acaacagctc gaccctacgc tgaggctaaa taaaggtctt gcgcacgtca
    12541 ccgccagggc tcagagcaca gaacccttct gggatgagcg ggtgatccgc cccatcgtga
    12601 gcgccaccca acggctggtc aaagaaatac agcatctgca aagcggcgac tttcgtctct
    12661 attgcctgta tgtggtcgcc gcactggttg tgctgctaat cgctattgcc gtctaaggaa
    12721 atcaccatga gacaaactct ttgcgacgga tatctggtca tttttgcgtt agcacaggcc
    12781 gtgattctgc tgatgctaac cccacttttt acgggtattt cccggcagat acgcgcgcgt
    12841 atgcactccc gccgcgggcc ggggatctgg caggattatc gcgatatcca caaactgttt
    12901 aaacgccagg aagttgcgcc gacatcttca ggtctgatgt tccgcctgat gccgtgggta
    12961 ttaatcagca gcatgctggt gctggcgatg gccttaccac tatttattac cgtttcccct
    13021 tttgcgggcg gcggcgatct gatcaccctt atctatcttc ttgccctgtt tcgttttttc
    13081 tttgctcttt ccgggctgga taccggaagt ccgtttgcgg gagtcggtgc cagtcgcgag
    13141 ttgacgctcg gcattctggt cgaaccaatg cttattctct cactgctggt attggcgctg
    13201 atagcaggtt ccacgcatat cgagatgatc agcaatacgc tggctatggg ctggaactcg
    13261 ccgctaacca ccgtactggc gttactggcc tgtggttttg cctgcttcat tgagatggga
    13321 aaaattccct ttgatgttgc tgaagcagaa caggaattac aggaaggccc gctgaccgaa
    13381 tattccggtg ccgggctggc gctagcgaaa tgggggctgg ggctgaaaca ggtcgtgatg
    13441 gcagcactgt ttgtggccct gtttctgccc tttgggcgcg cgcaagaact ttctctcgcc
    13501 tgcctgctga cttcacttgt cgttacgctg ctcaaggttt tgctgatttt tgtactggcc
    13561 tcaatcgcag aaaacacgct ggcacgcggg cgttttttac tcattcacca tgtgacctgg
    13621 cttggcttca gccttgctgc gcttgcatgg gtcttttggt taaccggtct gtaaggagca
    13681 ctgacggaat atggaaaatc ttgctctgac gacgttaatg ctgcctttta tcggcgcact
    13741 ggtcgtttcg ttttcgccac aacgtcgggc cgccgaatgg ggggttttgt tcgccgcgct
    13801 gaccacgctg tgcatgttgt cgctgatctc cgcgttttat caggccgata aagttgccgt
    13861 cacgttgacg ttggtcaacg tgggggatgt ggcattattt ggcctggtca ttgatcgcgt
    13921 gagtacgctg attctgtttg tggtggtgtt tctcggtttg ctggtcacga tctactccac
    13981 gggttatctg acggataaaa atcgcgaaca cccgcataac ggcacgaatc gttattacgc
    14041 atttttgctg gtgtttatcg gcgcgatggc gggactggta ctctcctcga cgctgctcgg
    14101 tcagttgttg ttttttgaaa ttacgggcgg ctgctcctgg gcgttgatca gttattacca
    14161 gagcgataaa gcgcagcgtt cagcactaaa agcgttgctt atcacccata tcggttcgtt
    14221 ggggttgtat cttgccgccg ccacgctgtt tttgcagacc ggaacgtttg tgcttagcgc
    14281 gatgagcgag ttacatggcg acgcacgtta tctggtttat ggcggcatcc tgtttgccgc
    14341 gtgggggaaa tcggcccagc taccgatgca agcgtggcta ccggacgcaa tggaagcgcc
    14401 aacaccgatc agcgcctatc tccacgccgc atcgatggtg aaagtgggcg tttacatttt
    14461 tgcccgcgcc attatcgacg gcggcaatat cccgcatgtg attggcggcg ttggcatggt
    14521 catgacactg gtcaccattc tttatggctt tctgatgtat ttgccacagc aggatatgaa
    14581 gcggttgctg gcctggtcga ccatcactca acttggctgg atgttcttcg gcttgtcgct
    14641 ctccatcttc ggctcgcggc tggcgctgga gggtagcatc gcctacatcg tcaaccacgc
    14701 gttcgctaaa agcctgtttt tccttgtagc aggtgcgctg agttacagct gcggcacgcg
    14761 cttgttgccg cgtctgcgtg gcgtattgca caccctgccg ttgccaggcg tgggtttctg
    14821 cgtggcagcg ctggcaatta ccggtgtgcc gccgttcaac ggcttcttca gtaaattccc
    14881 gctgtttgcc gccggttttg cgttgtcagt ggagtactgg atcctgctgc ccgccatgat
    14941 tctgctgatg attgaatcgg tcgccagttt cgcctggttt attcgctggt ttggtcgcgt
    15001 cgtgccgggc aaaccgagcg aggccgtcgc cgatgccgca ccgctgccag ggtcaatgcg
    15061 cctggtgttg attgtactga ttgtgatgtc gctgatttcc agcgtaatcg ccgcgacctg
    15121 gttgcagtaa ggagatgatg aatgaccggt tctatgatcg taaataatct ggcgggactg
    15181 atgatgctga catcgctgtt tgtgattagc gtcaaaagct atcgcctgtc atgcggattt
    15241 tacgcctgcc agtcgctggt gctggtgtct attttcgcca ctctctcgtg cctgttcgcc
    15301 acagagcaac tgctgatctg gtccgccagc gcctttatca ccaaagtgct gctggtaccg
    15361 ttaatcatga cttacgctgc acgaaatatt ccccagaaca tcccggaaaa agcgttattc
    15421 ggtccggcaa tgatggcact gctcgcggcg ttaattgtcc tgctttgcgc atttgtcgtt
    15481 cagcccgtga agctaccgat ggctaccggg ctgaaaccgg cgctggcggt agcgttaggt
    15541 cattttctgc ttggcctgct gtgcattgtc agccaacgca atatcctgcg gcaaattttt
    15601 ggttactgcc tgatggaaaa cggctcccat ctggtgctgg cgcttcttgc ctggcgagca
    15661 ccggaactgg tggaaatagg tatcgctacc gacgccatct tcgccgtcat tgtgatggtg
    15721 ttactggcaa gaaaaatatg gcgtacccac ggcacgctgg acgtgaacaa cttgaccgcg
    15781 ctgaagggat aatgagatga gttattctgt gatgttcgct ttactcctgc tcacgccgct
    15841 gcttttttcg ctgctctgtt ttgcctgccg gaaacggggg ctctctgcga cttgtacggt
    15901 gactgtatta catagcttag ggatcacact gctgctgatt ctagcactct gggtggtcca
    15961 aactgccgct gatgcaggag aaatattcgc tgcgggactg tggcttcata ttgatggtct
    16021 gggcggtttg ttcctcgcca ttcttggtgt gattggcttt ctcaccggtg tttactcgat
    16081 tggctacatg cgtcatgaag tggcacacgg cgagctttca cccgttacac tgtgcgatta
    16141 ctacggtttc ttccatctgt ttttgttcac catgctgctg gttgttacca gcaataacct
    16201 gattgtgatg tgggcggcga tcgaagccac caccttaagc tcggcgtttc tggtaggcat
    16261 ttacggtcag cgttcatcgc tggaatcagc atggaagtac atcattattt gtactgttgg
    16321 tgtcgctttt ggtctgttcg gtaccgtgct ggtatacgcc aacgccgcca gcgttatgcc
    16381 gcaggcagaa atggcgatat tctggagcga ggttcttaag caatcgtcct tgcttgaccc
    16441 aacattaatg ctgttggcct ttgtgtttgt gctaattggc tttggtacca aaaccgggct
    16501 atttcccatg cacgcctggc tgccggatgc tcacagtgaa gcgccgagtc cggtcagcgc
    16561 cctgctctcc gccgtattgc tgaactgcgc gctgttggtg ctgattcgct attacatcat
    16621 tatttgccag gccatcggca gcgatttccc caaccggttg ttgctcatct tcggcatgtt
    16681 gtcggttgcc gtggcggcat ttttcattct ggtacagcgg gacattaagc gtctgctggc
    16741 gtactccagc gtggagaaca tggggctggt cgcggtggcg ctaggcattg gcgggccgct
    16801 gggaattttt gccgcgctgc tgcacacctt aaaccacagt ctggcaaaaa cgctgctgtt
    16861 ctgcggttcc ggcaatgtac tgctcaagta cggcacgcgc gatctcaacg tcgtctgtgg
    16921 gatgctcaaa atcatgccat ttaccgccgt gctgtttggc ggcggtgcgc tggcgctggc
    16981 gctggcgctg gcagggatgc cgcccttcaa catttttctt agcgaattta tgaccgttac
    17041 cgcaggactg gcacgtaatc acctgctgat tatcgtcctg ctgttattgc tgttaacgct
    17101 ggtgctggcg ggcctggtac ggatggctgc gcgggtgtta atggcgaaac cgccgcaggc
    17161 cgttaaccgg ggtgatctcg gctggttgac cacctcgcca atggtgattc tgctggtcat
    17221 gatgctggcg atgggaacgc atattccaca acctgtcatc aggatcctgg cgggcgcttc
    17281 cactatagtc ctctcaggga cgcacgacct gcctgcacaa cgtagcacct ggcatgattt
    17341 tttgccttca ggcaccgcat ctgtttcgga gaaacacagt gaacgttaat tcatcgtcaa
    17401 atcgtggcga agcgattctc gccgccctga aaacgcagtt ccccggcgcg gtgctggatg
    17461 aagagcgaca aacgcctgaa caggtcacca ttacggtgaa aatcaatctg ctgcctgacg
    17521 ttgtacatta tctttattat caacatgatg gctggcttcc agtcctgttt ggcaacgacg
    17581 agcggacact taacggtcat tacgcggttt attatgccct ttcaatggaa ggggccgaaa
    17641 aatgctggat cgtggtgaag gcactggtcg atgccgacag tcgggagttt ccgtcagtca
    17701 caccgcgcgt ccctgccgcg gtctggggcg agcgagaaat tcgcgatatg tacgggctga
    17761 ttccggttgg cctgccggat cagcgtcgcc tggtgttgcc cgatgactgg ccggaagata
    17821 tgcatccgct gcgcaaagat gcgatggatt atcgactgcg ccctgaaccg acgacagatt
    17881 ccgaaacgta tgcgtttatc aatgagggca acagcgatgc gcgggtgatc cctgtcggcc
    17941 cgctgcatat cacctcctat gaaccgggtc acttccgctt gtttgtggat ggcgagcaaa
    18001 ttgtcgatgc tgattaccgc ctgttttatg tccatcgcgg catggagaaa ctggcagaaa
    18061 cgcggatggg ctacaacgaa gtgaccttct tatcggaccg cgtgtgtggg atttgcggtt
    18121 ttgcccacag tgtggcctat accaactcgg ttgaaaatgc actggggatt gaggtgccgc
    18181 aacgagcaca tactattcgc tcgattctgc tggaagtcga gcggctacac agtcatttgc
    18241 tcaaccttgg cctctcctgc cattttgttg gttttgatac cggctttatg caatttttcc
    18301 gcgtgcggga aaagtcgatg acgatggcgg aattgctgac cgggtcgcgt aaaacctacg
    18361 gtctgaatct gattggtggt gttcgccgcg atattctcaa agagcaacgt ctgcaaacgc
    18421 tgaaactggt gcgcgagatg cgcgccgacg tgtcggagct ggtagagatg ctgcttgcta
    18481 cgccgaatat ggaacaacgc actcagggca ttggcattct cgaccgacaa atcgcccgtg
    18541 attatagccc tgtagggccg ctgatccgcg cagtggtttt gcccgtgatt tgcgctttga
    18601 tcacccctac gccgactacg gcaatattcc aaaaacactg tttaccttta ccggcggcga
    18661 tgttttctcc cgcgtgatgg tccgtgtcaa agagacgttt gattcgctgg caatgctgga
    18721 atttgccctc gacaacatgc cggatacccc actgctgacc gaaggcttta gctataaacc
    18781 tcacgcattc gcgctgggct ttgttgaagc gccacgcggt gaagacgtgc actggagcat
    18841 gctcggtgat aaccaaaaat tgttccgctg gcgctgtcgt gccgccacct acgccaactg
    18901 gccggtgttg cgttacatgc tgcgcggcaa taccgtttct gacgcaccgc tgattatcgg
    18961 tagccttgat ccctgctact cctgtaccga ccgtgtgacg ctggtagatg tgcgcaagcg
    19021 ccagtcaaaa accgtgctgt ataaagagat cgaacgctac ggcattgatc gtaaccgttc
    19081 gccgctgaag taaggacaga agatgctgaa gttactgaaa actattatgc gcgccggaac
    19141 cgcgacggtg aaatatccct tcgcgccact ggaggtcagc ccaggctttc gcgggaaacc
    19201 ggacctgatg cccagccaat gtattgcctg cggtgcctgc gcctgtgctt gtccggcaaa
    19261 tgcgctgact atccagaccg acgaccagca aaattcgcgc acctggcagc tctatctggg
    19321 gcgttgtatt tactgcggac gttgtgaaga agtgtgcccg accagagcca tccagcttac
    19381 caataacttt gaactgaccg tcacccataa agccgatctc tatacccacg cgacgttcca
    19441 tctacaacgt tgcagccgtt gcgaacgccc gtttgccccg caaaaaaccg tcgcactggc
    19501 tgctgaattg ttggcacagc aacaaaatgc tccacaaaac cgcgaaatgt tatgggcgca
    19561 agcgagcgtc tgcccggagt gcaaacaacg cgcaacgctg atcaacgacg atacagatgt
    19621 accgctggtg gctaaggagc agctatgagt ccagtgctta cgcaatatgt cagccagccc
    19681 atcacgctgg acgagcaaac gcaaaagatg aagcggcatt tgctacagga tatccgtcgc
    19741 tcggcttacg tttatcgcat cgattgcggc ggctgcaacg cctgtgaaat cgaaattttt
    19801 gctgccatca cccccgtatt cgacgcagaa cgttttggca ttaaggttgt ttcatcaccg
    19861 cgtcacgccg atattttgtt atttactggc gcagtcaccc gggcgatgcg tatgcctgca
    19921 cttcgggcgt atgagtctgc ccccgatcat aaaatctgtg tttcctacgg cgcgtgcggt
    19981 gtcggcggcg gtattttcca cgatctctac agcgtctggg gcggcagcga caccattgtc
    20041 cccattgatg tttggatccc cggctgcccg ccaacaccgg ccgccaccat tcacggtttc
    20101 gccgtggcgc tcggtttgct gcaacagaag attcacgcgg tggattatcg cgatcccacc
    20161 ggggtgacta tgcaaccgtt gtggccgcag atcccgccat cacagcgtat cgccattgag
    20221 cgagaagcgc ggcggctggc gggctatcgt caggggcgag aaatttgcga tcggctcctg
    20281 cgccatttaa gcgacgatcc tacaggaaat cgggttaaca cctggttgcg cgatgccgac
    20341 gatccacgtc tcaatagtat cgttcagcaa ctctttcgcg tactccgggg gttacatgac
    20401 tgaagagtgc ggggaaattg ttttctggac gctgcgaaaa aagtttgtcg ccagtagcga
    20461 cgagatgccg gaacacagct ctcaggtaat gtattactcg ctggctatcg gtcatcacgt
    20521 tggcgtgatt gattgtctga atgtcgcctt ccgctgccca ctgacggaat acgaagattg
    20581 gcttgcactg gtcgaagagg agcaagcccg acgtaagatg ctgggggtga tgacttttgg
    20641 tgagattgtt attgacgcca gccacaccgc cctgttgacc cgggcattcg cgccactggc
    20701 agatgacgcg acgtctgtgt ggcaagcgcg tagcattcaa ttcattcatc tgttggatga
    20761 aattgtgctg gaaccggcta tctatctgat ggccagaaaa attgcgtgag aaggatttct
    20821 cattaataag gactgttgat ggctatgtca gacgaagcga tgtttgcccc gccgcaagga
    20881 ataacaattg aagcggtaaa cggaatgctc gcggagcggt tagcgcagaa acacggcaag
    20941 gcgtctttat tacgcgcctt catcccgctg ccgccgccgt tcagcccggt acaacttatt
    21001 gaactgcatg ttctcaaaag caacttctat taccgctacc atgatgatgg cagcgatgtg
    21061 acggcaacaa cagagtatca gggcgagatg gtcgattatt cgcgttacgc cgtccttctc
    21121 ggcagtagtg gaatggcgga gctacgcttt attcgtaccc acggcagccg ttttactccc
    21181 caggattgca cactgtttaa ctggctggca cgcattatca ccccggttct gcaatcatgg
    21241 ctcaatgatg aagcacagca ggtggcgctg cgtttgctgg agaaagatcg cgatcatcat
    21301 cgggtactgg tggatatcac taatgcagtg ctgtcacatc ttgatctcga cgatctgatc
    21361 gctgacgtcg ctcgtgagat ccatcatttt ttcggtctgg cttcagtcag tatggtactg
    21421 ggcgatcatc gaaagaacga gaagttcagc ctgtggtgca gcgatctttc tgcctcacat
    21481 tgtgcgtgtc tgccacacaa tatgcctggc gacagtgtat tgctgacaca aacgctacaa
    21541 acccgacaac cgaccttgac gcaccgtgca gatgatctgt ttctctggca acgcgacccg
    21601 ttattactct tacttgcatc taacggctgc gaatctgcgc tccttatacc gcttaccttt
    21661 ggcaaccata caccgggtgc attgttgctg gcgcatacct cttccactct ctttagtgag
    21721 gaaaactgcc agctactaca acacatagcc gatcgcatcg ctattgccgt tggcaatgcc
    21781 gatgcctgcc gtagaatgac cgatttgcag gaaagtttgc agcaagaaaa ccaccagctt
    21841 agcgagcagc tcctttcgaa tctgggcgtc ggtgacatta tctatcaaag ccaggcaatg
    21901 gaagacctgc tccagcaggt agatattgtg gcgaagagcg acagtacggt gttgatttgc
    21961 ggtgaaaccg gaaccggcaa agaggtgatc gccagagcga tccatcaact tagcccgcga
    22021 cacgacaagc cgctggtcaa aatcaactgc gctgccatcc ccgccagtct tctggaaagt
    22081 gagttattcg gtcatgacaa aggggcgttt actggtgcga ttaataccca tcgtggtcgt
    22141 tttgaaattg ccgatggcgg cacgttgttt ctcgatgaaa ttggcgatct gccgttagaa
    22201 cttcagccta aactgctgcg cgtattgcag gaacgggaga ttgagcgtct cggcgggagt
    22261 agaacgatcc cggtaaatgt cagagtcatt gccgccacca accgtgattt gtggcaaatg
    22321 gttgaagatc gccagtttcg cagcgatctc ttttatcgcc tgaatgtctt cccactggaa
    22381 ttgcctccgc tgcgcgaccg tccggaagat atccctcttt tagcaaagca tttcacgcaa
    22441 aaaatggcgc gccatatgaa tcgttcaatt gacgccatcc cgaccgaggc gctacgccag
    22501 ttgatgtcgt gggactggcc gggcaacgtg cgcgagctgg aaaacgtgat tgagcgagcg
    22561 gtactgctga ctcgcggtaa cagtctgaat ttacatctta atgtccgaca aagccgttta
    22621 ctgccgacgc taaatgaaga ttcagcgctt cgcagttcaa tggcgtagtt gctgcacccg
    22681 acgacgccag agaatgacga agaagaacgt cagcgcattg ttcaggtatt gcgagaaacc
    22741 aatggcattg ttgccgggcc ccgtggcgcg gcgacacgat tagggatgaa gcgcaccacg
    22801 ctgctgtcac gaatgcagcg tctgggaatc tcggttcgcg aggtgttgta atctgctttt
    22861 gcaggagtat gcatgagaaa caaactctct ttcgacttgc agttgagcgc cagaaaagcg
    22921 gcaatcgctg aacggattgc cgcccataaa attgcccgca gtaaagtgtc ggtcttttta
    22981 atggcgatgt ccgctggcgt gtttatggcg atgtccgctg gcgtgtttat ggcgatcgga
    23041 tttacttttt acctttccgt tatcgccgat gccccgtctt cataggcatt aacccatctg
    23101 gtgggcggcc tttgctttac acttggcttt attttgctgg cggtttgcgg caccagcctg
    23161 ttcacctcgt cggtaatgac ggtaatggca aaaagtcggg gcgttattag ttggcgaact
    23221 tggctgatta acgcacttct ggtggcctgc ggtaatctgg caggtattgc ctgtttcagt
    23281 ttgttaatct ggttttccgg gctggtgatg agtgaaaacg cgatgtgggg agtcgcgatt
    23341 ttacactgcg ccgagggcaa aatgcatcat acatttactg aatctgtcag cctcggcatt
    23401 atgtgcaatc tgatggtttg cctggcgctg tggatgagtt attgcggacg ttcgttatgc
    23461 gacaaaatcg tcgccatgat tttgtccatc accctgtttg tcgccagtgg ctttgagcac
    23521 tgtatcgcca atttgtttgt gattccgttc gccattgcca ttcgccattt cgcccctacc
    23581 tccttctggc aactggcgca cagtagcgca gacaattttc cggcactgac ggtcagccat
    23641 tttattaccg ccaatctgct cccggtgatg ctgggtaata ttatcggcgg tgcagtgctg
    23701 gtgagtattt gttatcgggc tatttattta cgtcaggaat cctgaaaaat cagcccggcg
    23761 aaacagttcg tcgggctatt tatttacgtc aggaatcctg aaaaatcagc ccggcgaaac
    23821 agttcgtcgg gctgaagatt attcttgcgc gatttgccca tcgggccagg cgtggatcac
    23881 ggctttgatc agcgtcgcca atggaatggc gaagaatacg ccccagaatc cccacaaacc
    23941 accgaagatc accaccgata aaataatcac cagcggatgc aggttaaccg cttcggagaa
    24001 caacaccggt actagcaggt tgccgtccag cgcctgaata atcagataca ccgcgaagca
    24061 gctccagaat tcagtacctg cgccaaactg gaatagcgcc acgccaacca ccggaatggt
    24121 caccacaaat gcgccaatgt acggaataag aaccgagaag ccgaccagca ctgccagcag
    24181 cagcgaatag ttcagcccga agagcaagaa ccccagccag gtggcgatcc ccaccacgat
    24241 catctccagc actttgccgc ggatatagtt ggtgatttgt tgattcatct ccttccacac
    24301 ctgtcctgcc agcccacggt tacgcggcag cacccggcga acggcgttca gcatctgctc
    24361 tttgtctttc agcaggaaga agaccatcaa tggcaccagc accagataga cggctatggt
    24421 cagcaaaccg accagcgagg cgagggaaat tttcaccacc gaatcgccca tggtcagcat
    24481 ccgactgcgc atattttcgg ccattgcatc aataatgccc gcgtccatta acaccggata
    24541 gcggcgcggc aacgtggcgg caaagtcaga aagcttattg agcatccccg gcatatcgcg
    24601 gattaagtag atgccctgtt gccaggcgat aggcagtacc acgaacgcca tcagtagcaa
    24661 tataccgaca aaaaccacca atacaatcga cgtcgcccag cggcgggagc agccaataga
    24721 ttgcaggcgc acggttggcc attccagcaa ataagccagc acaatagcca ccagcaacgg
    24781 agcaagcagg ccactaaaga aaaagataat gccaaatccg gcaactaaaa taaccagcaa
    24841 ggcaatcgct tccgggtcgc taaaacggcg gcgataccat tgcatcaaca tttcgagcat
    24901 aaagaccttc cctgaacctc aagagcggga ttgcgatccg caattgtatc gaaatgtcac
    24961 aaaaaagact tcgcttttta tgacggattc aggaaactga aaagtcattt gagtgggcta
    25021 atcttcgccg ttacactcaa aggcggcgag gtgggaacga tatttcacag tatcggtcaa
    25081 atgactatct tcagaaatac aggatagagg ttatgttcag gcagttgaaa aaaaacctgg
    25141 ttgcaaccct cattgctgct atgaccattg gtcaggtagc cccggcgttt gccgacagcg
    25201 cagacacctt gccggatatg ggaacctccg caggaagcac gctttccatt ggtcaggaaa
    25261 tgcagatggg cgactattat gtccgccagc tacgcggcag cgcgccgtta attaatgacc
    25321 cgctgttaac gcaatatatt aattcgctgg ggatgcgtct ggtttcgcat gccaattcgg
    25381 ttaagacacc gtttcatttt tttctgatca acaacgacga aattaacgcc tttgctttct
    25441 ttggcggcaa cgtggtgctg cactctgccc tgttccgtta ttccgataac gaaagtcaac
    25501 tggcttcagt tatggcgcac gaaatctccc acgtcaccca acgtcacctg gcgcgagcga
    25561 tggaagatca acaacgcagc gcgccgctga cctgggtcgg cgcgttaggt tctattttac
    25621 tggcgatggc cagtccgcag gcggggatgg cggcgctgac cggtacactg gcgggaacgc
    25681 gccaggggat gatcagtttc acccagcaaa atgaacagga agcggaccgc attggtattc
    25741 aggtgctgca acgctcggga ttcgatccac aggcgatgcc gaccttcctg gaaaaattgc
    25801 tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt attgactcac ccgttgccgg
    25861 aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat gcgcccgatg gtggtgcagt
    25921 cgtcggaaga tttctatctg gcgaaagcgc gcacactggg gatgtataat tccggacgta
    25981 accagctcac cagtgatttg ctggatgaat gggcgaaagg aaacgttcgt cagcaacgag
    26041 cggcgcaata tggtcgtgct ttacaggcga tggaagccaa taaatacgac gaggcgcgta
    26101 aaacgctgca accgttactg gcggcagaac ctggcaacgc atggtatctc gatctggcta
    26161 ccgatatcga tcttgggcaa aacaaagcca atgaggcaat caatcgcctg aaaaatgccc
    26221 gcgatttacg caccaatccg gtgttgcagc tcaacctggc gaacgcttat ctgcaaggcg
    26281 gtcaaccaca agaagcggcc aatattctga atcgctacac ctttaataat aaagatgaca
    26341 gcaacggctg ggatttgctg gcacaggcgg aagccgcgct aaataaccgc gatcaggagc
    26401 tggctgcgcg agcagaaggt tatgcgctcg ccggacgact cgatcaggcc atttcgctgt
    26461 tgagtagcgc cagttcgcag gtgaaattag gcagcctgca acaagcgcgt tacgatgcgc
    26521 gcatcgacca gttgcgccag ctgcagaaac gctttaagcc ttataccaag atgtaattcg
    26581 cattaaagga agaacaatga ccaaacaggt aaaaatctac cataacccac gctgttcaaa
    26641 gagccgggaa acgctgaacc tgctgaaaga aaacggcgtg gagccagaag tggtgctcta
    26701 ccttgagaca cccgccgatg cggcaacgct gcgcgatttg ctgaaaatgc tggggatgaa
    26761 tagcgcccgt gaattgatgc gccagaaaga ggatctttat aaagaactaa acctggctga
    26821 cagttcgctt agcgaagagg cgctgattca ggcaatggtg gataatccga agctgatgga
    26881 gcgcccgatt atggtcgcga atggcaaagc gcggattggt cgtccaccgg agcaggtact
    26941 ggaaatcgtg ggttaagatg tgctccatcg ctagttgaag cacattgccg gatgcgacgc
    27001 ttgccacgtc ttatccggcc tacgaatact atggattaac ttcgcgtagg tcaggcgttc
    27061 gcgccgcatc cgacaataaa caccttatct acaacttcag aatttctttc acaaacggaa
    27121 tggtcagctt acgttgcgcg gtaatcgacg cacgatccaa ctgatccaac gtcataaata
    27181 gcgtgcgcat ttctctgtcg agccgcttca gcaagaaacg ccccacatct tccggcagtt
    27241 caaaaccacg caaacgcgcg cgtaactgta gcgcctgcaa cttatcttca tcagaaagtg
    27301 gctgcaattt gtagatctgc ccccagtcga gacgcgacgc gagatccggt aatcccagat
    27361 tcaactgccg cggtggacga tcgccggtga tcaacaaccg tgttttgccc gattccagaa
    27421 ttcgattgta gagatcgaaa atcgccatct cccacaactc atcgcctgca atacactcaa
    27481 tgttgtcgat acagaccagc gacaaatgct ccataccgtc gagcacttcc ggaacaaacc
    27541 aggtgcgttt atccagcggg acatagccca ccgcatcgcc acgctgcgac aattccgcgc
    27601 aagccgcgtg cagcagatgg ctgcgccccg cgccttcgcg tgcccagaga tagatgtaac
    27661 cgctatgttc ctgacgcagc acgttttgca gcgcggccag taaagaggag ttatcccccg
    27721 gccagaaact tgcaaaggtt tcgtcgtcag gaagataaag tggcaaagag agctgtgccg
    27781 gtgtgttcag agatacctca accaggattt cacaaaatcg cgagaagttt accacagaat
    27841 cccatgatgt ttgaaccggg cagcaacact gcccggtcgg tacattattt gtctgttata
    27901 tccgcgtctt ctgcgtccag caccacttct tcagggcgca gcacactgat cagtttgaaa
    27961 atcaaactta agccgatacc gacgatggtc gccagcgcca taccttttaa ctccgccgcg
    28021 ccgatgttta ccttcgcgcc actgacgccg atgatcaaaa tcacggaagt caggatcagg
    28081 ttctgtgctt tgttgtaatc cactttcgat tcgatcaaaa cacgaatacc ggaagcacca
    28141 atgacaccat aaagcagcag cgaaacgccg cccatcaccg gcaatgggat catctggata
    28201 gcggcagcca gtttaccgac gcaggaaagc aggatagcga aaatcgccgc cccgccgata
    28261 acccaggtac tgtaaacacg ggtgatcgcc atcacgccaa tgttttctcc gtaagtggta
    28321 tttggcgtag agccaaagaa gccggagatc acggtcgaca ggccgttagc aaacatcgaa
    28381 cggtgcagac ctggatcgcg cagcagatct tttttgacga tattagccgt tactaccagg
    28441 tgccctacgt gttcggcaat aaccactaac gccgctggca gaatagtcag aatggcaaac
    28501 cactcgaagc gcggcgtata gagggttggc agtgcaaacc agtgagcatt aataatcggc
    28561 gtggtatcga caattcccat tgcgaaagag agcgcgtacc ccaccagcac gccaattaaa
    28621 atcgggataa ttgccaggaa accacgaaac agcacggaac ctaaaaccgt gaccgccagg
    28681 gtggtaatag agatgatgat ggttttggag tttggcgttt gcccttcagc cgggagtaaa
    28741 cccgccatac cggcagctac gcccgccagc tccagaccga tgacggcaac gattgcgccc
    28801 attgccgcag gtggaaacag cacgtccaac cagccggtcc ccgctttctt cacgataaaa
    28861 gaaaccaggc agaacagcac gccgcacata ataaagccgc ccagcgcgac ttcataccct
    28921 aacggcaaca gtaacaatac cggtgaaata aaggcaaagc tggaaccaag ataagccgga
    28981 attttccctt tacagatgaa gagatacagc agcgttccaa taccgttaaa taacagtaca
    29041 gtcgccgggt taatatgaaa taagacgggc accaggacgg ttgcaccaaa catggcgaac
    29101 aaatgttgca aactaagcgg gattgtctgt aaaagtggcg gtctttcact caccccgata
    29161 gcacggcgcg tcatagtatt atcctctgta ttatgtgtta taggcgcttt actcaaaaaa
    29221 aagccgactc ttaaagtcgg ctttaattat ttttattctt tatttcgtac caaagatttt
    29281 gtcaccggca tcgccgaggc ccggaataat gtatccgtgc tcgttcagtc cctggtcaat
    29341 cgatgcagta tacagttcga cgtccgggtg cgctttttcc agcgcagcga taccttctgg
    29401 cgcagcgacc agcaccagaa ctttgatgct gctgcagccc gcttttttca gcaggtcgat
    29461 ggtcgcgata acggaaccac cggttgccag cattgggtca acgatcagcg ccatacgctc
    29521 atcgatgtta gaaaccagtt tctggaagta cggtaccggc tccagcgttt cttcattacg
    29581 gtacataccg acaacgctga tgcgcgcgct cggaacgttt tccagcacac cgtccatcat
    29641 accaagaccc gcacgcagaa ttggcacaac ggtaattttc ttacctttga tctggtcgat
    29701 ttctaccggg ccgttccagc cttcgatagt tactttttcc gtttcgaggt cggcggtcgc
    29761 ttcgtaagtc agcaggctac ccacttcgga agcgagttcg cgaaagcgct tggtgctgat
    29821 atcttgctca cgcatcagtc ccagcttgtg tttgacgagt gggtgtttga cttccacgat
    29881 cttcatactc tttctccttt gaggggcagc cacaaaaaaa tcgacggatt atacctcctt
    29941 tcttca
//
LOCUS       DAZPAB010000029        29396 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZPAB010000029 DAZPAB010000000
VERSION     DAZPAB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.029"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(1..441)
                     /locus_tag="ACHUPG_001158"
                     /pseudo
     CDS             complement(1..441)
                     /locus_tag="ACHUPG_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(416..1216)
                     /gene="otsB"
                     /locus_tag="ACHUPG_001159"
     CDS             complement(416..1216)
                     /gene="otsB"
                     /locus_tag="ACHUPG_001159"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR0860855.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1383..2369)
                     /gene="araH"
                     /locus_tag="ACHUPG_001160"
     CDS             complement(1383..2369)
                     /gene="araH"
                     /locus_tag="ACHUPG_001160"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR0860856.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2384..3898)
                     /gene="araG"
                     /locus_tag="ACHUPG_001161"
     CDS             complement(2384..3898)
                     /gene="araG"
                     /locus_tag="ACHUPG_001161"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR0860857.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3968..4957)
                     /gene="araF"
                     /locus_tag="ACHUPG_001162"
                     /pseudo
     CDS             complement(3968..4957)
                     /gene="araF"
                     /locus_tag="ACHUPG_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5754..6257
                     /locus_tag="ACHUPG_001163"
     CDS             5754..6257
                     /locus_tag="ACHUPG_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR0860858.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6337..6588)
                     /gene="yecJ"
                     /locus_tag="ACHUPG_001164"
     CDS             complement(6337..6588)
                     /gene="yecJ"
                     /locus_tag="ACHUPG_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR0860859.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="ACHUPG_001165"
     CDS             complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="ACHUPG_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR0860860.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7052..7375
                     /locus_tag="ACHUPG_001166"
     CDS             7052..7375
                     /locus_tag="ACHUPG_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR0860861.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7546..8043
                     /gene="ftnA"
                     /locus_tag="ACHUPG_001167"
     CDS             7546..8043
                     /gene="ftnA"
                     /locus_tag="ACHUPG_001167"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR0860862.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8080..8319)
                     /locus_tag="ACHUPG_001168"
     CDS             complement(8080..8319)
                     /locus_tag="ACHUPG_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR0860863.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8509..9720
                     /gene="tyrP"
                     /locus_tag="ACHUPG_001169"
     CDS             8509..9720
                     /gene="tyrP"
                     /locus_tag="ACHUPG_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR0860864.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="ACHUPG_001170"
     CDS             complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="ACHUPG_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR0860865.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10643..10729)
                     /locus_tag="ACHUPG_001171"
     tRNA            complement(10643..10729)
                     /locus_tag="ACHUPG_001171"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10693..10695),aa:Leu,seq:taa)
     gene            complement(10742..10815)
                     /locus_tag="ACHUPG_001172"
     tRNA            complement(10742..10815)
                     /locus_tag="ACHUPG_001172"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10781..10783),aa:Cys,seq:gca)
     gene            complement(10870..10945)
                     /locus_tag="ACHUPG_001173"
     tRNA            complement(10870..10945)
                     /locus_tag="ACHUPG_001173"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10910..10912),aa:Gly,seq:gcc)
     gene            complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="ACHUPG_001174"
     CDS             complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="ACHUPG_001174"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR0860866.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="ACHUPG_001175"
     CDS             complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="ACHUPG_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR0860867.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="ACHUPG_001176"
     CDS             complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="ACHUPG_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR0860868.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14329..14400
                     /locus_tag="ACHUPG_001177"
                     /pseudo
     CDS             14329..14400
                     /locus_tag="ACHUPG_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            14483..14659
                     /gene="yecU"
                     /locus_tag="ACHUPG_001178"
     CDS             14483..14659
                     /gene="yecU"
                     /locus_tag="ACHUPG_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR0860869.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            14646..14870
                     /gene="yecF"
                     /locus_tag="ACHUPG_001179"
     CDS             14646..14870
                     /gene="yecF"
                     /locus_tag="ACHUPG_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR0860870.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="ACHUPG_001180"
                     /pseudo
     CDS             complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="ACHUPG_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(15753..16505)
                     /gene="tcyN"
                     /locus_tag="ACHUPG_001181"
     CDS             complement(15753..16505)
                     /gene="tcyN"
                     /locus_tag="ACHUPG_001181"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR0860871.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="ACHUPG_001182"
     CDS             complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="ACHUPG_001182"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR0860872.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="ACHUPG_001183"
     CDS             complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="ACHUPG_001183"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR0860873.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="ACHUPG_001184"
     CDS             complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="ACHUPG_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0860874.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="ACHUPG_001185"
     CDS             complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="ACHUPG_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFR0860875.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="ACHUPG_001186"
                     /pseudo
     CDS             complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="ACHUPG_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="ACHUPG_001187"
     CDS             complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="ACHUPG_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HFR0860876.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22563..23969
                     /gene="fliD"
                     /locus_tag="ACHUPG_001188"
     CDS             22563..23969
                     /gene="fliD"
                     /locus_tag="ACHUPG_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR0860877.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            23994..24404
                     /gene="fliS"
                     /locus_tag="ACHUPG_001189"
     CDS             23994..24404
                     /gene="fliS"
                     /locus_tag="ACHUPG_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR0860878.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            24404..24769
                     /gene="fliT"
                     /locus_tag="ACHUPG_001190"
     CDS             24404..24769
                     /gene="fliT"
                     /locus_tag="ACHUPG_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR0860879.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            24847..26334
                     /gene="amyA"
                     /locus_tag="ACHUPG_001191"
     CDS             24847..26334
                     /gene="amyA"
                     /locus_tag="ACHUPG_001191"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR0860880.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26368..26781)
                     /gene="yedD"
                     /locus_tag="ACHUPG_001192"
     CDS             complement(26368..26781)
                     /gene="yedD"
                     /locus_tag="ACHUPG_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR0860881.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            26968..28173
                     /gene="yedE"
                     /locus_tag="ACHUPG_001193"
     CDS             26968..28173
                     /gene="yedE"
                     /locus_tag="ACHUPG_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR0860882.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            28170..28403
                     /gene="yedF"
                     /locus_tag="ACHUPG_001194"
     CDS             28170..28403
                     /gene="yedF"
                     /locus_tag="ACHUPG_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR0860883.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            28510..29180
                     /locus_tag="ACHUPG_001195"
                     /pseudo
     CDS             28510..29180
                     /locus_tag="ACHUPG_001195"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(29220..>29396)
                     /locus_tag="ACHUPG_001196"
     CDS             complement(29220..>29396)
                     /locus_tag="ACHUPG_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR0860884.1"
                     /translation="RLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGR
                     SLKQEPTEMIQATA"
BASE COUNT         7320 a         7587 c         6997 g         7492 t
ORIGIN      
        1 caatgcgatt attcacccca cgtttgcgta attcatgcgc aaagggcaac aggtgataat
       61 cgtggatcca gataatgtca tcgtcttgca acagcggcag taatttatct gccagcaacg
      121 catttacgcg tagatagccg tcccaggcag gacgctgaaa ttgcaccaga tcgagccgat
      181 aatgaaaagc gggccagaga acggcattgg agaattggtt gtagtattcg tcaaggtcct
      241 gttcgctgag gttaaaagag gcccacgtaa tgttaccttt tttagcggct gatcctcatt
      301 ccctgtttca ccactccagc caaaccacag tccgcctgcg gctttcagtg cccccagtat
      361 gccaacggca aggccaccgg cactggcggc gtgctcgtct ggtggtgcaa tccggttaga
      421 tactacgact aaacgactca tagtcatcac tcctgttatt ttctcttttt tgttgtaatg
      481 cggcggttat catttcaagc cagctccaga catccggcac acccgccagt cgccatgatg
      541 cctgagttgc acctgtgcca atttttactg acattccgcc cagtcggtta acgactgcga
      601 agccagattc atcggttaaa tcatcgccca gaaatacggg cgttcgcccg ataaaaggag
      661 cttcctgcat aaaggctgca atcgcttcac ctttactggt acctctcggt ttgatctcga
      721 caacacactt tccctgctgt aacgccattt gtggccagat ctgagtaata cgttgcgcta
      781 atgtcattaa tgcgtcttca tgctgcggag cctgacgata atgcagcgca aaagccatcc
      841 ctttcgcctc cagctccgcg ccgggatact gagcgatgac cgtatgcagt tgcacgctaa
      901 tatcacgcgc aatcgcatcc ggcagatgaa cgatatgtgt tttaccattg atgtcacggc
      961 gctccgcccc atgcacgccc gctaacggga agcgataagg ttttgccagt gcgtcaagct
     1021 ccaccattga gcgccctgat atcaatgcca atgcaccatc acttgcggtt gccagtagct
     1081 gtagtccttg cagaatattg tcaggcacga cgacctgatc gggatgcggt ttgatttccg
     1141 ccagcgttcc atcaagatca aaaaaccagg catatttcgc ggatagttca ggggtttcgg
     1201 ttaacggttc tgtcacccgg ctctcctcct tctcttttct tattgtgttg cgcagaccgc
     1261 gctttacaga catgtaagta tagacagtgt gacggaggtc gccatttgca acgaagaaca
     1321 gccaaccgtt gtggcggctg gctgtggtgg gaaaaaacgt taagttgttg tggaaaaaag
     1381 catcagacag tgcgcttcgc tttttgcttg taacggtcga agatcaccgc tgccagcagg
     1441 attaagccgc gaaccacgta ctgcgcgaaa ggagaaatat taagcaggtt catggcgttt
     1501 tccacggtgc ctaaaattaa gatacccgcc accacatatg agatttttcc gatgccacct
     1561 ttcagagaaa cgccacctaa aacgcaggcg gagataacaa taagctcata accaatcgac
     1621 gtcattggct gcccactagt catacgtgaa gccagaataa ttccggctat cgcagatacc
     1681 aggccagaga gaacaaagat aataattttg gtgcgaacaa ccggtacacc cgccagacgc
     1741 gcagcctctt cgttcccgcc aatcgccagg gtattacgac caaaggtggt tttattcagc
     1801 agcaaaccaa agataatcag acacgcgacg gtgagccaga ttggcgcagg cagaccgaac
     1861 cagttagcgt aaccaagggc aaagaagctt tcatcttcga taccgaccgc tttaccgtct
     1921 gaaatgatgt acgccagacc tcgaacaatc tgcatcgttg ccagtgtcgt gatcagagca
     1981 tttattttca gtttggcgat aacaaagcca ttgaccaggc cacagagaac gcccagcaac
     2041 aaacccgctg ccacgccaat ccacaggctt tcagtcaggt tgataaccac cgccgtggtg
     2101 acacccgcac aggcaattac ggaggcgaca gaaaggtcaa agtcaccgga agcaaggcag
     2161 aacaacatgc cacacgccac catccccgac atggaaattg ccaggcccaa ccctttcata
     2221 ttaatgaagg tggcaaaatt tgggacaaaa atggcacagg cgataaagag caccgcaaac
     2281 accaccagca tgccgtactg atcccagata cgcccgaagc tgaatgacga cttaggtgcg
     2341 ccagaccccg atgtagaaac agaagacatc atactctcct tactcaggca actgcctggc
     2401 tgactttagg catcgcaagg ctcagtgcct gacgctcatc tgcctgctcg tgtaacaatt
     2461 caccggcgat ttcgccttcc cgcatcacca caatccggtc ggcaacgccg aggacttcag
     2521 gtaagtcgct ggaggcaaac agcaccgcca cgccctgcgc cgccagcgca taaattacgt
     2581 tatatatttc gtgcttagcg ccaacatcaa tgccgcgcgt aggttcatcc agcaaaatga
     2641 ccttcatctc ttccgataac cagcggccca gaatggcttt ttgctgattt ccgcctgaga
     2701 gattcatgat cagttgctcc gcgcccggcg ttttgatgtt gagcgaacga atgtgctgat
     2761 cggcattgtt ttcttcccaa ctgttgttga ttacacaacc gccgagcaca tgtttgcgtc
     2821 tggcactgat gttgatattg tcgcgaacgg agtgcacggg aataatgcct tccgctttgc
     2881 gagcttccgg gcagagcatc atgcctgcgg caatggcgtg gctcggttta cgaatatcga
     2941 tcggctgttg gtcgatataa acctgaccgg cggtgatttg cgtcccgcca aacaagcctt
     3001 tcattaattc gctacgcccc gcccctacca gaccaaacag cccaacaatt tcaccactgc
     3061 gaaccgccag acttattggc gtacgcacgc ctagtgcttt cacagcatca agacgcaggc
     3121 gctcctcgcc ataactacga ggttgccagc cgtagatatc gccaatgtcg cgcccgacca
     3181 tcgcctgcac cagcgcgtcg tggtcaacct gctgcatatc ggtaaaggtt ttgacataac
     3241 gtccatcttt aaagacagtg atggcatcgc tgagggcaaa tatttcttcc atacggtgag
     3301 aaacgtataa gatcacccgc ccctcttttc gcagttcacg aataacgcgg aaaagattgc
     3361 cgatttcgcg ggcagaaagg gagctggttg gctcatcaaa ggcgataatt ttagcgttac
     3421 gcgccagcgc tttggcgatt tcaaccatct gccactgacc aatggcgaga tatttcagcg
     3481 gcgtgtccgg gtcaatatcc ataccaagat gtttaagttg taaacccgcc tcataattca
     3541 gcaatgagcg attcacaatg ccgcctttat gcggcagctg gccgagatag atgttttccg
     3601 cgacggtcat ttccggcacg agatgcagtt cctggtaaat aatcgccaca cccgcgttaa
     3661 gtgctgcggt cgtgtcggaa aaggacattt cctgcccatt aatcactaca gaacccgtgg
     3721 ttggcgcata gttgccgctg aggattttta agagagttga ttttcctgcg ccattttcac
     3781 ccatcaacgc atgaacctga ccggcatagc agtcaaaact aatatccgtc agcgccttaa
     3841 cgccgggaaa cgttttaccg atgccgcgaa atgagagata cggggtagac tgttgcataa
     3901 cgtctccgtg aatcactggt cgttcacacc ccctctgcat catgcagagg ggaatttttc
     3961 cggcaaatta cttaccgcct aaaccttttt tctccagttc ttctttaaag ttgtcacgcg
     4021 tgatcagtac cacgtctgta acttcggtaa attttggcgt ttcaacgtct tttgctaccc
     4081 agttgtaaag catttcgctg gatttatagc catgtacgtc cgggcttggc agcagggaac
     4141 cgtagaagcc ggttgcctgt gctttagaca gttcgcttac tgcatccaca ccgttaatac
     4201 caataccgat gatatcggct gctttaaagc cctgaccttc ggttgcacgt acgccgccaa
     4261 gtacggtgct gtcgttcata ccgacgatca gccaatgttt aacttccgga tgttgaacca
     4321 gcattgagtt ggcagcgtca aatgcccccg ggatgtcgtt agatttggta ggtacctgat
     4381 aaatttgttt ttccgggaat cctgcagctt tcagtgcgtc catagaaccc gttgtacggc
     4441 ggcgggcggt atccagttcg ttggcggtaa tcgccatcac tgcgctttct ttgacatccc
     4501 agccacgttt ctgcatctct ttatacagtt cctagccctg acgttcgcca attttagtcg
     4561 ccgccatcat caccagagga acggtatcca ttggcttacc tttggcgtta acaaactggt
     4621 catccactgc aatgactttc atatcgtagc cacgcgcttt cgcgacgatg gcagagccga
     4681 gtttcgggtc cggagtacaa ataacgaaac cttttgcgcc actggctgcc aggctgtcga
     4741 tcgcgttcaa tgttttttcg ccatccggca cggcaatctt aataacctca aaccctaaat
     4801 ccttcccggc tttatcggca aacttccatt cggtctggaa ccacggctct tccggttgct
     4861 tcaccagaaa accgagcttc aggttctccg ccatagcgga ttgtgacata acggctgcca
     4921 gaccaatggc tgccagggct ttagtaaatt tgtgcatggt tctctccagc tttagtgtcg
     4981 ttttgtgtag ggcaaaaacg aatgacattc gttaaattaa tcggaaaaca aagcattacc
     5041 ttttatctaa aagataagtg accgtgttga catagtttta gcgagaaatt aattctccat
     5101 aggagagcaa tatcacatcg cagaattaca gtgagaacgt gcataaattt agcgggaaaa
     5161 gacataagga aaagccaatt tgtcagacaa attgttgaat gcacagcaga ttaatccata
     5221 agattagcct ggaaatcctt gttgtcttcg gtacccatgc gggatgtctt ctttttaacc
     5281 agtcaatagg ccgcattacc tggcgttgag tttttgaaat ggtgtaataa ccacaactca
     5341 aagatgtgga aaatgcgcgt cattcatttc gccattaatt atcactgtgc tcattaatta
     5401 acagaacacg tataatgaga gccatctcgc aaaaatgaaa aaacgtttta taaaatcatc
     5461 acttcatcat gaattcaaat tcatcgatta atatcaacaa gatacaaaaa gcactatcat
     5521 taaaattcat tgcatttaca ttgatttcac caatgaaatg taaaaatata taaacttgat
     5581 gatttaagca ttttcttata accgttcaga cgttattctt atttcagatc atcgtcagaa
     5641 ttgactccac gatcacattt cggaccggca gaaaggaatt attctgcaaa cagtaattat
     5701 ggtgttttga tttatcttgc acctctccac ttctggatat aaggatatta ggtatggcaa
     5761 ccgctggaat gcttctcaaa ctcaactctc aaatgaaccg cgagttttac gcatccaatc
     5821 tctaccttca cctgagtaac tggtgttctg aacagagtct gaacggcacc gccactttcc
     5881 ttcgcgccca ggcacagagt aatgtgaccc aaatgatgcg catgtttaac tttatgaaga
     5941 gtgtcggcgc tacccccatc gttaaagcca ttgatgttcc aggtgaaaaa ctgaactctc
     6001 tggaagaact gttccaaaaa acgatggaag aatacgagca acgttcaagc actctggcgc
     6061 agttagccga tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc ctgcgcgatc
     6121 tggaaaaaga acagcagcat gatggcctgt tgctgcaaac cattcttgat gaagtgcgca
     6181 gtgcgaaact tgcgggtatg tgccctgtgc agaccgacca acatgttctg aatgtcgtgt
     6241 cacaccagct gcattgatca tacatcggcg ctaatacatt gcgccgatga agtttttgag
     6301 aaaccgctgc ctcatctgtt tgaagcagcg gtttttttaa tgggattcac cctgtggggt
     6361 aaacttgagt tcaatcagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc
     6421 ggcagcggga tgatgagtaa agtcgatatt cttcagctga ctggacattt tttcacgaac
     6481 ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc
     6541 taccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg
     6601 atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac
     6661 cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc
     6721 tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg
     6781 cagtttcatc gcggcactcc gaccatttgt tatacaggtg ttattgtctt tgcgcttata
     6841 atatgacaac catcacaaaa atcaatcttt atgtgataca aatcacataa ataccccttt
     6901 aatgttataa aaatgataat caaaaaacag cccccctatt tctgacacct acagatggca
     6961 agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcgctgta acgcgtaaaa
     7021 tagtgctttc tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt
     7081 aataacgctg gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc
     7141 aacaggtatt gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg
     7201 ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcgat
     7261 cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac
     7321 ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt
     7381 ttaatcagcg aggggatctt cgctgattaa agaaatagcg gtaatgcgtt ttattcccat
     7441 tcgtattttt aataattgaa gtttatattt taccttttgc aaataataaa ataacaaatt
     7501 atagtgacgc cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa
     7561 atgattgaaa aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag
     7621 caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt
     7681 cacgcccagg aagagatgac gcatatgcag cgtctgtttg attacctgac tgataccggc
     7741 aatttaccgc gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa
     7801 ttattccagg aaacctataa acacgaacaa ttaatcaccc agaaaattaa cgaactggct
     7861 catgctgcaa tgaccaatca ggactaccca acatttaatt tcctgcagtg gtatgtttct
     7921 gagcagcatg aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc
     7981 aaaagcggcg aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac
     8041 taatgctctc cggcggcagg tttctgccgc cgtttcaaat cagtgacggc aaatcttact
     8101 ctgatcggtc gaaaaacctt cttctgaagg tataaatttg ccttttgctg ccagaaacgc
     8161 taccagctct cccgctgtca ccccttctgc cgagcacgtg tgaaaacgtg cctgttcacc
     8221 aaaacgcgct ttaatcgcag cttccagact ggcatgcgta tattgctcgc ctgattcaat
     8281 catcatattt aacacttcat gaccgtgaat agagtccatc gttcctcctc aaaaaaagcc
     8341 tagcgtagcg attaccgctt atgaagactt tgcgccagcg caggactgaa tgcttttatt
     8401 gtacatttat atttacacaa tatgtaacgt cggtttgacg aagcagccgt tatgccttaa
     8461 cctgcgccgc agatatcact cataaagatc gtcaggacag aagaaagcgt gaaaaacaga
     8521 accctgggaa gtgtttttat cgtggcggga accacaattg gcgcaggcat gctggcaatg
     8581 ccgctggctg cggccggtgt tggttttagc gttacgttaa tcttgttgat tgggctttgg
     8641 gcgttgatgt gctacacggc gctattactg ctggaggtgt accagcatgt tccggcagat
     8701 accggtctgg gcacgctggc aaaacgctat ctgggacgct acggtcaatg gctgacgggc
     8761 ttcagtatga tgttcttaat gtatgctctg actgcagcat acatcagcgg tgccggtgaa
     8821 ttgttggcct ccagcatcag cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg
     8881 ctgttgttca cttttgttgc cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta
     8941 tttaaccgtt ttctgttcag cgccaaaatt atttttctgg tggtgatgct ggtattgctg
     9001 ctaccgcata ttcacaaagt gaatctttta accctgccgt tgcaacaggg gctggcactg
     9061 tctgcaatcc cggtgatttt tacgtcgttt ggttttcacg gtagcgtgcc gagtattgtc
     9121 agctatatgg atggcaacat tcgtaagcta cgctgggtgt ttataatcgg tagtgccatc
     9181 cccctggtgg catatatttt ctggcaggtg gcgacgcttg gcagcattga ttcaacaacc
     9241 tttatgggat tgctggctaa tcatgctgga ttaaacgggc tgttacaggc gttacgcgaa
     9301 atggtggcct ctccgcatgt tgagctggcg gtgcatttat ttgctgattt agccctcgcc
     9361 acgtcatttc tcggcgttgc gttaggctta tttgattatc tggctgattt gtttcagcgt
     9421 tcaaataccg ttggtggacg attgcaaact ggtgcaatta cctttctgcc gccgttggcg
     9481 tttgcactgt tttatccacg aggatttgtg atggcgctgg gttacgccgg tgtggcgctg
     9541 gcggtactgg cattgattat cccttcacta ttgacctggc aaagcagaaa gcacaatcct
     9601 caggcgggtt accgggtcaa aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt
     9661 attgctgtga ttggcgtgca atttttgatt gcggcagggt tgttaccaga agtggggtga
     9721 tcagatagcc tcaaattcct tattgggtgc cagaattaac gctgacaccc aatgtgacct
     9781 cttaatgcag gcagcactgc ttaaatttct taccactgcc gcacggacaa ggatcgttac
     9841 gccccggttt ctcttctgct ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca
     9901 tccagtatgc atgtaaatca agcgccgcca gtcgaatggc atctacgctc tcttcaaacg
     9961 cttctggcga cattttctct acccgctcga agttttcctc agtaccgtgc agcgcaatcg
    10021 cctccagcgc tggttttaaa gaatcgggca acgttgacca gtcagaaagt gccacgcccc
    10081 gcatatagcc aaagcaccac tcctcaacaa tcgtcagctc gctgccatca acttctcgca
    10141 agccgaataa cggctcaaac tgctccggga attcgttcag acgctctgcg gtatcggcca
    10201 tatgttgaaa agccaggttc ataaagcgcg tcatctcttt ctctgacgcc caacgcggca
    10261 catagtcagc cccaccccac acggcaacca gccactgttc tggttcaatc tcttgcggag
    10321 aactcaacac cgccgtcaat aaaccgtcca gctccgccac atcaaggatg gcgtggtcag
    10381 tgttgtattt ggtcagaata tcgtccagcc attccaactc actttcgttt aacggtcccg
    10441 ttttcatacg cttttccttg tggatctcaa ctcgccagca cctatcttac atgccggtcc
    10501 gtatcagaga tactttttga gtggctttgc cggtgattaa aaattaagga gggtgtaacg
    10561 acaagttgca ggcacaaaaa aaccacccga aggtggtttc acgacactgc ttattacttt
    10621 gattttattc ttatctttcc catggtaccc ggagcgggac ttgaacccgc acagcgcgaa
    10681 cgccgaggga ttttaaatcc cttgtgtcta ccgattccac catccgggct cgggaagaaa
    10741 gtggaggcgc gttccggagt cgaaccggac tagacggatt tgcaatccgc tacataaccg
    10801 ctttgttaac gcgccaaatt cttcaggcct ttcagccaga catccgcttg acgccgatgt
    10861 cttttaaact ggagcgggaa acgagactcg aactcgagac ctcgaccttg gcaaggtcgt
    10921 gctctaccaa ctgagctatt cccgcattca tcaagcaatc agttaatcac ttgattttat
    10981 tatcgtctgg caatcagtgc cgccgttcga tgcgttgcat tctacttacc tggcgcgatg
    11041 agtcaacgat atttttcacc acttttgatc gtttgctgaa aattacgccg aaacgatcac
    11101 tgatcaagca aatctgcacg cgcagcgctc aaatattgca acattgacca cagagtcagt
    11161 accgcagcca caaagaaaag tgcaataccg acgtactcaa cccaaatgtt cggacgccac
    11221 agcagccagg ccaacgccac catctgggca gtggttttca ctttcccaat ccaggagacg
    11281 gccacgctac tgcgtttacc caactccgcc atccattcgc gtagcgcaga aataataatt
    11341 tcacgggcga tcatcgttgc cgccggtaat gtcacccacc agctgtgata atgctcggtt
    11401 accagcacca tggcgatagc cacgagaact ttatctgcca cagggtcaag gaaagcacca
    11461 aaccgggtac tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc
    11521 gcgacgcaga aaatgagcgc ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc
    11581 aatacaaaga atgggataag gatgacacgg aacagtgtaa gcaacgtagg gatattaaat
    11641 tgcataatga cgggtaacta tctgttgtca gtaagattac ccctatgttg ctacagagac
    11701 atcaatgttt caacgaccag aagatctttt ctgccagacc ttgcgaaata cccggcactt
    11761 ttgcaatttc ctcgacgctg gcgttacgta aaccttgcaa accgcccata tatttcaaca
    11821 acatttgccg acgttttggc ccgacgcctt caatggtttc cagggaactg gtatttttga
    11881 ccttcgcccg ttttttacgg tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat
    11941 gctggataac atgcagcgcg ggtgaatctg gcggcaaact aaatccctca ccttccggct
    12001 caaagaacag cgtttccagt ccagccttac gatctgctcc tttggcaacg ccaagtagca
    12061 gcggatgatt tttatcccat gagacatcca gttcggcgaa gacatttttc gcctgcgcaa
    12121 gctggccttt gccgccgtcg ataaggatca catccgggat cttactgtcg tcaatggctt
    12181 taccataacg ccgacgcagc acctgattca tcgccgcata atcatcgccc ggcgtgatgc
    12241 ctgtaatgtt atagcgccga tactccgcac gcagcgggcc gttagcatca aacaccacac
    12301 aggaagcgac ggtttgttcg cccatggtat ggctgatgtc aaagcactcc atccgcttca
    12361 cttccggcaa tttcaacacg ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct
    12421 gcgaaagttt gctggttaag gccgtcgccg cattggtgcg cgcgagtttc agataacgcg
    12481 ccctatcgcc gcgaggtttg gtttgaacat taatcttgcg tcccgccagt tctgaaaggg
    12541 aatcggcgag cagcgtttta tcgctaagat taaaatcgag caggatctca cccggtaagg
    12601 tgcgcatctg gctgccttgt aaatagaact gacctacgaa ggtttctacc acctcgctca
    12661 gttccgtacc gccaggcact ttcgggaaat agctgcggct gccgagcact ttgccctgac
    12721 gaatgaacaa tacgtggaca caagccatgc ccgcatcgaa cgccacacca ataacgtcaa
    12781 ggtcgtcgcc ggtattggaa acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt
    12841 ggtcgcgaat acatgccgct tcttcaaact ccagattctg gctggcagtt tccatacggc
    12901 taatgagttg cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc acatactcga
    12961 cctgctgagc gtattcttct tcactcacca gtccttcaac gcacggtccc agacagcgcc
    13021 ctatctggta ttgcagacac ggacgcgagc gattgcgata aacactattt tcgcactggc
    13081 gaatggggaa aatcttttgc agtagcgcca gtgtttcacg tacggcatag ccattcggga
    13141 acgggccgaa atattcacct ttggcatgct ttgcaccacg atgcatcgcc agacgcgggt
    13201 gggtatcgcc actcaggaag ataaaaggat aagatttatc atcgcgtagc aaaacgttgt
    13261 aacgcggctg atagagtttg atgtagttgt gttccagcaa cagcgcttcg gtttctgtat
    13321 gcgtaaccgt tacatcaatt tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag
    13381 cgaggttgct gcggaaatag ctggaaagcc gttttttcag gtctttcgct ttgccgacat
    13441 agataaccgt accaccagca tcgtacatgc gataaacgcc tggctggctg gttacggttt
    13501 ttaaaaacgc ttttgcgtca aactgatcac tcactgactt gataatgtct ccgcattaca
    13561 cagaccatgg cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact
    13621 gaacatacga tagcggtagc tgttcaccgt tttcggactg agattgagct gttctgagat
    13681 ctcattgacc ttctggccct tagtgatcat cagcataatc tgcaattcac gttcagacaa
    13741 actggcaaat gggctttctg ttttttctgg ttcgatctgg cttaacgcca tttgttgagc
    13801 gatatcagaa gcaatgtaac gctgccctga atagacagaa cgaatcgcac tcacgacttc
    13861 ctgcggagcc gcgcctttgc tgaggtagcc agcagcacct gcctgcatga ctttcgctgg
    13921 taaagggttt tctgtatgga ccgtaagcat gatgatttta acatcagctg tggaacgcgc
    13981 gattttacgc gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca ttagcaccac
    14041 gtcaacggca tttgtccggc accacttaac ggcgtcttca ccgcacgatg cctctccgac
    14101 gacttttata cccttaatat cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg
    14161 gtcatcaaca agtagaacgt tgatcaaagg aatatctcca gaaataggga taacgctact
    14221 gatagttagt cattcgtata ttagcggttt ttattgcaac tttgaaacgt taaaaatggt
    14281 gggtattcga tttttctctc gtttttgtca attcgattgt ccacagttat ggtcacgccc
    14341 gtcaccaacg gcttaccgga tcgtttcaca gtaagcccta cgatgaaatg tgacaaaaat
    14401 tgactttatt cagcaaaaat gaaaatcagc ccgctgaata tgtaacatta attaacccga
    14461 acaatacggg caaaaaacat tcatgataaa aatatttatt ggtcattata ttaacgtatt
    14521 ttatagcact gccgatatca cgctcaaaaa acaaccactg ctatttttag caaagcttat
    14581 ggtatactcc gccgccttaa catttttcac cacaaatttt cattgcaaca tgacgaggaa
    14641 aataaatgag tacgcctgat ttttctactg ccgagaataa tcaagaactg gcaaatgaag
    14701 tctcctgcct gaaagcgatg ctgacgctga tgctgcaggc gatgggacaa gctgacgcgg
    14761 gccgcgtgat gttaaagatg gaaaaacagc ttgcgctgat cgaagacgaa acccaggctg
    14821 cagtattttc caaaacggtt aagcaaatta aacaagccta ccgtcagtaa tataaaaccg
    14881 gctgagagcg tgcctttcag ccggttattg acaaaatcat cgctatctct gctgatgtct
    14941 tcccttccgc cgtccatttc agaatttctt tttcgcgctt gctgaaattc atctctggcg
    15001 tcatcactat ttcatcattt aaacgcatca gagccatcag actttcgcgc accagtaact
    15061 gcatttttaa ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg caacgggaaa
    15121 aggacaaaaa gcccagcgcc cgatttggca gcattaaata ctgagtgaca ccgcggcgta
    15181 aaccatgtgc gtgcgcggct tcccataatg gctgtgcttc gctgaataag tcatcattcc
    15241 acattaaatg gccctgacta aagttttcag ggttcagcac aggatcaatt gcgagaaaat
    15301 tttttgcctg ataataacta acccacgact cagggtaatt ggtgtagaaa gccactttag
    15361 gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata gtaatcgtac tccagctgct
    15421 gagcctgaag ctctatttca tggtaggcct cttctgcggc ctccatcctc tgaaaacgca
    15481 acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc
    15541 ctgagagtga gtatcattta taatgataat tcaaactatc acataaataa gatttatata
    15601 taatttatat tattcaggca atgaattact tttgcaagcc atcgcattct cttatgttat
    15661 taatgagtta tgctgatttg ttaagcagtt ttatcacgct tgaaatggcg tcccgccccg
    15721 acaggtgaat cgtcggggct gattttttct tattattgca gcagaagctt ctcgaggaac
    15781 tggcgggtgc gaggctgctg gggatcggca aataacgctt ttgctgcccc ctgctcgact
    15841 atccgcccct ggtccataaa gatcgcccgg tccgcaacat cccgggcaaa gctcatttcg
    15901 tgtgtcacaa tcaccatcgt gcgcttttcc tgcgccagct ggcggatggt gttcaggact
    15961 tcgcccacca gctctggatc cagcgccgac gttggctcgt caaacaaaat cacctcagga
    16021 cgcattgcca gtgcacgcgc aatcgcaaca cgctgctgtt gaccgccaga caaacgacgt
    16081 ggatagctgg tttctttacc ggccagccca actttcgcca gcagctcgcg agcgcgcgcc
    16141 gtggcctctt ctttcggttc gcctttgacg atcaccggcc cttcaataat gttttccagc
    16201 accgtacgat gcggaaacaa attaaagttc tggaagacaa acccgacgtg ctgacgcaac
    16261 tggcgaatca gcgatttttg ctgacttaat gaacgtgcag tatcaatagt gatatcgcca
    16321 acggtgatcg ttcccgcttc cggttgttcc agcagattta tgctacgtag caatgtggtt
    16381 ttgccggaac cactcggacc gataattgcc accacttcgc caggctttac ctcgaggtcg
    16441 ataccgtgca gcaccgtctg accgtggaat tttttcacca ggttcttaac ttcaatggca
    16501 ctcattttgg ttctctctcc tggcgattaa gttgattctc aaaatggttc tgcaacgtcg
    16561 ataacacagt cgccatgatc cagtagatga gcgaagccgc caggtacatg gtgaacacct
    16621 ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag ctccggcacc tggattgtcg
    16681 cggccagcga cgtatctttt accagactga taaagctgtt cgacagcggc ggcaacgcca
    16741 cccgcgccgc ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc ataccaatac
    16801 tggcagcggc ttcccactga cctttatcaa tagaagaaat ggcggcgcgc agcgtttcgg
    16861 cggcataggc ggcagtgttc agcgacagac caatcattgc tgacggaatg ggatctaatt
    16921 cgataccaaa ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg agcggcgtac
    16981 cacgaaaaat agagatataa aaacgcgcca gccagcgcac gggccagatt ggcgacaggc
    17041 gcatcagcgc gagaataaac ccgagtagta agccaaaaaa catgccgccg atactgagtt
    17101 gcagggtata cccagcccct ttcaacagga acggcaagga atcaataacc agttgtatac
    17161 tttcttgcat gagcgttccc ggagctaaac gtggggatga taggcgaaca gcgcaggcgc
    17221 gccgccggta tgaataaaca gaatcggccc ttcatctttg aagcgtttct gactgatacc
    17281 gtcaatcagc cccgccatcg cttttccggt atacacagga tcaagcagaa tgccttccag
    17341 acgcgccagc aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc
    17401 aaaatagtca tcccagagta aaatttccgc tgatgcggtc agctccagtt ctttcgcaat
    17461 cgcctgttgt aggttaacca ctttcggcaa ttgatcggca acggaacgcg acacggtcac
    17521 gccaatcagt tcgctttcag gcatcaggtg ttccagccca acagccagtc cggcgtgagt
    17581 tccggcactg cccgatgcga ctaccaccga cgaaatatta accgcccctt cacactgttg
    17641 cgcgatttcc agcgcactct ccacataacc tagcgcgcca agagcattag aaccgccaac
    17701 cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc gtcgccagtt cttccagttg
    17761 ggcattggga tcggtcagtg cgtcgcacat ttcaatctgg gtattgaaca gatccagcaa
    17821 caaacgattg ccgttggtta aatagttttc tgcggttgtg ccaataggat tttccagcag
    17881 cgccacgcag tgcagaccga gtttcgccgc gactgcggca gtctggcgca catggttaga
    17941 ctggatcgcg ccggcagtaa tcagcgtatc ggcaccttcg cgcagggcat ctgccgcgag
    18001 aaattccagc ttacgtaatt tattgccgcc cattgccatg ggggtgacct catcccgttt
    18061 gatgaaaatt tcccgtccaa gataatcaga aaagcgcggc agatattcga gcggcgttgg
    18121 cgcgccgata aactccaacc gtggaaaacg ggttaaatta tgcagtggca taacagcctc
    18181 cgatgtgtgt tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc
    18241 gctagtgaaa gcgccctttt ttgtcattat gctgattatt tggtcacatc agcaccaaac
    18301 catttttcgg aaagagcttg cagagtgccg tctttttgca tttccgcaat tgcatcattc
    18361 accgctttca gcaggtcttc atttccttta cgcagcgcca cgccagactc ctgacgggag
    18421 aatgcttcac cggttactgc cagcgtatcg ttggttttct tcaccagatc cagcgccgcc
    18481 agacgatcaa cgaggatcgc atcgatacgc cctacgcgca gatcctgata tttggtcggg
    18541 tcatcatcat aggtacgcac atcgacaccc tgaacattct gccgcagcca ctcttcatag
    18601 ttggtgccca gaccgacacc cacttttttg cctttcagat cagcagccgt tttaatggtg
    18661 ccttcgttac cttttttcac cagcgcctga ataccggaaa tggtgtaagg ggtagagaaa
    18721 tcgtattttt tcttgcgctc gtcagaaatg gtgacctgat taatcaccac atcaatgcgt
    18781 ttagagtcca gcgacgccag cataccgtcc catttggtcg gttttagtga cgcctcaacg
    18841 ccaagatgtt ttgccagctg ttgggcaaat tccacttcaa aaccggttaa tttgccgtca
    18901 tccccctgaa aactgaacgg cggataagtg ccttccagcc ctaccagcag cgtgccgctc
    18961 tctttgactt tattaagcag accttcatct gcaaaacttt taacgctcat gcccgcaacc
    19021 agcgccacgg ccatcacacc catcaatgcc tgacgtccca gatgtgctaa tttcatattc
    19081 accccgaatg ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca
    19141 actccgctac atcttattct tatttaatat atatcagaag caggcaggct ggaggatttc
    19201 tgcacaagtc cggtacaggt ttggcgctga tagtgttgat acttccgtaa tgcgatgcgg
    19261 taattgttgg tgctggtggt gggcagccac ggggcgagga tttcatcaag gaaaccgtcc
    19321 tgcagctgat cgagggaaat attttgctca tgtagatgat tacccaaacg gcgcagacga
    19381 acgacatatt cacgcaccgt acctgggctc atttcagttt gctcaaaaag aaatttctta
    19441 aagccgataa tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg
    19501 cacaatgccg cccatgattg ttgttccgtt tgccagcctt tttcatcaag cagcgtgtcc
    19561 aggcgggaaa tctcgatttt attcactatt ttgccgtcgc gaactaaggt aatgcgatcg
    19621 agtaatttac ggcaatgcgc gcaatgggtc tggctgtgtt taaagtcttt aagatagcgg
    19681 cttaatggcc gtcttttcag gtgctgcacc atcattaaga actcctggta gtcaaagtta
    19741 aagtgcggca tttactgacg ttataactta cccagtttag tgcgtaaccg tttaatgtcc
    19801 tggctgtgta actgactgac ccgcgattcc ccgacctcca gcaccgcgcc aatctctttg
    19861 agattcagct cttcctggta atagagggtt aataccagtt tttcgcgctc cggcaacgtt
    19921 tcgatggctt ccatcacccg ctggcgcaga ttactgtcca gcagttgttg tagcgggttt
    19981 tctcgctgat gatcatcagt aaccagttcg atgctatcgc cgtgctcttc acgccactca
    20041 tcgtaggaga agagctggct gttattggtg tcgagcagca tttgacgata atcggcaata
    20101 tcgatcccta aacgttccgc tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc
    20161 agttgcccta ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag
    20221 tcacggctgc gaagttcatc cagcatagcg ccacggatac gctacactgc gtaagttgta
    20281 aatgccgttc cttgtagggc gtcatagcgt tcgacagcat taagtaaccc aatgccgccc
    20341 gcctgtagca gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct
    20401 tcgtgacgca ccagcgggac ataacgctgc cacagcgagt gtttatccat tacaccttca
    20461 gcggtataga gtgaattcac gataaacagc cctgcgttat atgagttatc ggcatgatta
    20521 tccgtttctg cagggttttt aatcggacga ttagtgggtg aaatgagggg ttatttgggg
    20581 gttacgcaaa aagccgaatg taaaaaaccc cgccggtggc ggggtttgag aggcattcag
    20641 acgattaacc ctgcagcaga gacagaacct gctgcggtac ctggttggct tttgccagca
    20701 cggagttacc ggcctgctgg ataatctgcg ctttcgacat gttggacact tcggtcgcat
    20761 agtcggcgtc ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg ttgttcaggt
    20821 tggtgactgc ggaatccaaa cggttttgca ccgcacccag ggaggagcgg aatttgtcga
    20881 tggagctgat ggcttcatcc agagctttca ggggatcggc tgtcgtagct gcttttgttt
    20941 cagcatctgt cgtcaattta ccatcagcat ctttataaac tgccttaccg ctgccgttag
    21001 tcactgaacc atcatcctgt aggtaaaaat ctacagtatc agatgtggtc aacgcctcgt
    21061 tgttatcaac ataaaccgaa tcaacacctt ttgttacagc gccagttgtg ccgttgacgc
    21121 tataacctgc agtagtgaaa tcaacattgt ttcccgaggt tgatacagct ttatcgaaag
    21181 ctgcctgatc aacttttgca cctgttactg aataagtaat agtgtccggc gtagtactat
    21241 taccggtaaa ggagatagag gtaccgccaa tgtcaatggt tgtcgctttg gtattattgg
    21301 ctgcaagtgt ggacagacta gcctcagtca aagaagcacc agagccgttt ttcgttaagc
    21361 gcccagttgt atcaatgtag agtttatctc cattgcttgc cgtaatttta ccatcactgc
    21421 tgatctgaac atcctgtgca gaaccatcga tagtaatagt gcccttagcg gtatcaccaa
    21481 ctcccggggt caaatatttc tgaacatcgg cagctgaagc cgttgtggta tcaaaagagt
    21541 atgacttact tgcactgtca tacttgtaat tcgttgcact agccgccgtt ccgaagccgt
    21601 tattaatggt tgcctgaaca gtatcaccat cactaactcc agccagcaaa tcagacgctt
    21661 tagcagcatc gtaacccgca ctcacagtat atttgttgcc gaccactgtt gcattagcag
    21721 ctaccaagtc agctttagat gcagcagtat tagccacagc cccgccacca ttcacattaa
    21781 acccattcag ccccagcgta tcagagtcaa ttttcttcag atcaatagtg atagtctggc
    21841 cgtcattcgc accaacctga attttcatcg aaccgtcttt tgccagtacg ttcacgccgt
    21901 tgaactgggt ctgaccggat acgcggtcaa tttcgtcgag acgggatttg atttcgtcct
    21961 gaatggagtc cagatccgaa tcagagttag tcccggtaga agcctgaacc gtcagctcac
    22021 ggatacgctg taagttgttg ttgatttcgg acagcgcgcc ttcagtggtc tgtgcaacag
    22081 aaataccgtc gttggcgtta cgtgcagcct gagtcaggcc tttaatgtta gaagtaaaac
    22141 ggttagcaat cgcctgaccc gcggcgtcat ccttcgcgct gttaatacgc aagccagaag
    22201 acagacgctc gatagaactc gacagcgcag actggttctt gttgatatta ttttgagtga
    22261 tcagcgagag gctgttggta ttaatgactt gtgccatgat tcgttatcct atattgcaag
    22321 tcgttgatta cgttttgggt ttccacccgt cggctcaatc gccgtcaacc ctgttatcgt
    22381 ctgtcgtaaa acaaccttta gaattttttt cacaaacagc cattttttgt tagtcgacga
    22441 aatactcttt tctctgcccc ttattcccgc tattaaaaaa aacaattaaa cgtaaacttt
    22501 gcgcaattca ggccgataac cccggtattc gttttacgtg tcgaaagata aaaggaaatc
    22561 gcatggcaag tatttcatcg ctgggagtcg ggtcaggtct ggatttaagt tcaatccttg
    22621 atagcctcac cgccgcgcaa aaagcgacgc taacccctat ttcaaatcag caatcgtcgt
    22681 ttaccgctaa acttagcgcc tacggtacgc tgaaaagcgc gctgacgact ttccagaccg
    22741 ccaatactgc attgtctaaa gccgatcttt tttccgccac cagcaccacc agcagcacca
    22801 ccgcgttcag tgccaccacc gcgggtaacg ccatcgccgg gaaatacacc atcagcgtca
    22861 cccatctggc gcaggcgcaa accctgacca cgcgcaccac cagagatgat acgaaaacgg
    22921 cgatcgccac cagcgacagc aaactcacca ttcaacaagg cgacgacaaa gatccgatta
    22981 ccattgatat cagcgcggct aactcatcgt taagcgggat ccgtgatgcc atcaacaacg
    23041 caaaagcggg cgtaagtgcg agcatcatta acgtgggtaa cggtgaatat cgtctgtcag
    23101 tcacatcaaa tgacaccggc cttgataatg cgatgacact ctcggtcagc ggtgatgatg
    23161 cgctacaaag ttttatgggc tatgacgcca gtgccagcag caacggtatg gaggtctcgg
    23221 tcgccgccca gaatgcgcag ctgacggtca acaacgtcgc catcgagaac agcagcaaca
    23281 ctatcagcga cgcgctggaa aacatcactc tgaacctgaa cgatgtcacc acgggcaacc
    23341 agacgctaac catcactcag gacacctcca aagtgcaaac ggcgattaaa gactgggtga
    23401 atgcctataa ctcgctaata gataccttca gcagcctgac caaatacacc gccgtagatg
    23461 cgggagctga tagccagagt tctagcaatg gcgcactgct cggcgactcc acgctgcgga
    23521 cgattcagac gcagttgaag tcgatgctga gtaataccgt cagttcttcc agctataaaa
    23581 cgctggcgca gattggtatc acgaccgatc ccagcgatgg caaactggaa ctggatgccg
    23641 acaaactcac cgctgcactg aaaaaagatg ccagcggcgt aggtgcattg attgttggcg
    23701 atggtaaaaa aaccggcatc acaaccacca tcggcagcaa cctgaccagt tggctttcga
    23761 caacgggcat tattaaagcc gctaccgatg gcgttagtaa aaccctgaat aagttaacta
    23821 aagactacaa cgccgccagc gatcgcattg atgcgcaggt cgcgcgctac aaagaacaat
    23881 ttacccaact ggacgtttta atgacctcgt taaacagcac cagcagctac ttaacgcagc
    23941 agttcgaaaa caacagtaat tccaagtaat cattattcat cgggagacag gtcatgtacg
    24001 cggcaaaagg cacccaggcc tatgcacaaa ttggcgtcga aagcaccgta atgagcgcca
    24061 gccaacagca gttggtcacc atgctatttg atggagtgct gagcgcactg gttagagcgc
    24121 gactgtttat gcaggacaac aatcagcaag gcaaaggcgt ctctttgtca aaagcgatca
    24181 acatcattga gaacggactg cgggtgagtc ttgatgaaga gagcaaagac gaattaaccc
    24241 aaaacttaat tgctctttat agctatatgg tcaggcgttt gctgcaagcc aatttacgca
    24301 acgatgtctc agcagtcgaa gaggtggaag cattaatgcg taatattgcc gatgcctgga
    24361 aagagtcttt actctcccct tcttcgattc aggacccagt ctgatgaaca atgcaccgca
    24421 tttgtatttc gcctggcaac aactcgtcga aaaaagccag ctcatgttac gcctggcaac
    24481 ggaagaacaa tgggacgaac tcatcgctag cgaaatggcg tatgtgaatg cggtgcagga
    24541 gatcgcacac ctgactgaag aggttgcccc gtccactacg atgcaggagc agctccgccc
    24601 gatgctgcac ctgattctcg acaacgaaag caaggtaaag cagttattac agattcggat
    24661 ggatgaactg gcgaaactgg tcggtcagtc atcggtgcaa aaatcggtgt taagtgccta
    24721 tggcgatcag ggcggctttg tgctggctcc gcaggataac ctcttttgaa tcttaatgag
    24781 ccgatggctc gcgaataatc cgattacggc tacgcttcta atattcccct tgaatggagt
    24841 cgaagaatgc gtaatcccac gctgttacaa tgttttcact ggtattaccc ggaaggcggt
    24901 aagctctggc ctgaactggc cgagcgcgca gacggtttta atgatattgg tatcaatatg
    24961 gtctggttgc cgcccgccta taaaggcgca tcgggcggct attcggtcgg ctacgactcc
    25021 tatgatttat ttgatctagg tgagtttgat cagaaaggca gcatccctac taaatatggt
    25081 gataaagcac aactgctggc cgccattgac gccctgaaac gtaatgacat tgcggtgctg
    25141 ctggacgtgg tagtcaacca caaaatgggc gcggatgaaa aagaagctat tcgcgtgcag
    25201 cgtgtaaatg ctgatgaccg tacgcaaatt gacgaagaaa tcattgagtg tgaaggctgg
    25261 acgcgttaca ccttccccgc tcgtgccggg caatactcgc agtttatctg ggatttcaaa
    25321 tgttttagcg gtatcgacca tatcgaacac cctgatgaag atggcatttt taaaattgtt
    25381 aacgactata ccggagaagg ctggaacgat caggttgatg atgaactcgg taatttcgat
    25441 tatctgatgg gtgagaatat cgattttcgc aatcatgccg tgacggaaga gattaaatac
    25501 tgggcgcgct gggtgatgga acaaacgcaa tgcgacggct ttcgtcttga tgcggtcaaa
    25561 catattccgg catggtttta taaagagtgg atcgaacacg tacaggaagt tgcgccaaag
    25621 ccgctgttta ttgtggctga atgctggtcg catgaagtcg ataaactgca aacgtatatt
    25681 gatcaggtgg aaggcaaaac catgctgttt gatgcgccgc tgcagatgaa attccatgaa
    25741 gcatcgcgca tgggtcgcga ctacgacatg acgcagatct tcactgggac gctggtggaa
    25801 gccgatcctt ttcacgccgt aaccctcgtt gccaaccacg acacccaacc gttgcaagcc
    25861 ctcgaagcac cggtcgaacc gtggtttaaa ccgctggcgt atgccttaat tttgttgcgg
    25921 gaaaatggcg ttccttcggt gttctatccg gacctctacg gtgcacatta cgaagatgtc
    25981 ggtggtgacg ggcaaaccta tccgatagat atgccaataa tcgaacagct tgatgagtta
    26041 attctcgccc gtcagcgttt cgcccacggt gtacagacat tatttttcga ccatccgaac
    26101 tgcattgcct ttagccgcag tggcaccgac gaatatcccg gctgcgtggt ggtcatgtcg
    26161 aacggagatg atggcgaaaa aaccattcat ctgggagaga attacggcaa taaaacctgg
    26221 cgtgattttc tggggaaccg gcaagagagc gtagtgacag acgaaaacgg cgaagcgacc
    26281 ttcttttgca acggcggcag cgtcagcgtg tgggttatcg aagaggtgat ttaaatccat
    26341 ccccggcggc aagccgggga gatttcatta cggcagtggc gtcggtaacg gcgctttatc
    26401 cagcgcggcg gcacactctg ccgtcgggcg atccacacgc tccatcgtca taccatcgta
    26461 ctcaatggtg ttaccgtcac gctcaacctc gtacagctca cgttttacgg taacgttagt
    26521 gagatcatca gacatcaatg tcaacttacc cggcacggca atcccccgct gccactgacg
    26581 gcaatccaag gtatcgccct ctttagtgac aatcaggctg cctatcgctt ccggactgac
    26641 cagcgcacgt tgcggccctt tggtctgcca gtagcccgcc agccagtccg gcgcgggggt
    26701 tttcacgaca ttgttatagt tttcgacttc ggcacagccc gccagcaaca tgagcgcgcc
    26761 tgcaattgcg agttttttca tcattcgtcc tgcatgagaa gaaaagagga ttgtggcatt
    26821 aaagctctga tgtcgccagc ctttccaggt tcagagagaa atttgatcta actattattt
    26881 ttgtgaatta ttaccccgaa aataaatttg catggtgtat gattcgcggc tcttgtttca
    26941 aaccttctga gttcagaggc tacattcatg tcatggcagc aattcaaaca cgcctggttg
    27001 attaaattct gggcacccat ccctgcggtc atcgcggcgg gtattctctc cacttactat
    27061 tttggcatta ctggcacctt ttgggctgtc acgggtgaat ttacccgttg gggcggtcag
    27121 ctcctgcagc tgtttggcgt ccatgctgaa gagtggggtt attttaaaat tatccatctg
    27181 gaaggatcgc cattaacccg gatcgacggc atgatgatcc tcggtatgtt tggcggctgc
    27241 tttgctgctg cgctgtgggc caacaatgtc aaactacgca tgccacgcag ccgtatccgc
    27301 attatgcagg ccatcattgg cggcattatc gccggttttg gcgcgcgtct ggcaatgggc
    27361 tgtaacctgg cggcgttctt taccggcatt cctcagttct cgctgcatgc ctggttcttt
    27421 gccatcgcca ctgccattgg ttcatggttt ggcgcgcgct ttaccctgct gcccatcttc
    27481 cgtattcccg tgaaaatgca gaaagtttct gccgcctcac cgctgacgca aaaaccggat
    27541 caggcgcggc gtcgttttcg tctcgggatg ctggtctttt tcggcatgct gggctgggcg
    27601 ctgctcacgg cgatgaacca accaaaactg gggctggcaa tgctgtttgg cgtcggcttt
    27661 ggtttactga ttgaacgtgc gcaaatctgc tttacttcgg cgttccgcga tatgtggatc
    27721 accggacgta cccatatggc gaaagcaatc attatcggta tggcggtgag tgccatcggg
    27781 atcttcagtt acgtacagtt aggcgttgaa cctaaaatca tgtgggcggg accaaatgcg
    27841 gtaattggtg gtttactttt tggttttggc atcgtgctgg caggcggctg cgaaaccggc
    27901 tggatgtacc gcgcggtaga aggccaggtg cactactggt gggtcggtct gggcaacgtg
    27961 atcggctcaa cgattctggc gtattactgg gatgatttcg ctccggcgct ggccaccgac
    28021 tgggacaaaa tcaacctgct gaaaactttt ggtccgatgg gaggcctgct ggtgacatat
    28081 ttgctgctgt ttgctgcgct gatgttgatt atcggctggg aaaaacgctt cttccgccgt
    28141 gaggcaccgc agactgctaa ggagatcgca tgaaaaatat cgttcctgat taccgccttg
    28201 atatggtggg tgaaccctgc ccttatccgg cagtcgcaac ccttgaggcg atgccgcaat
    28261 taaaaaaagg tgagatcctg gaagtggtga gcgactgtcc gcagtcgatc aataatattc
    28321 cactggatgc gcgtaatcac ggctacacgg tgctggatat tcagcaagac gggccgacca
    28381 ttcgttattt aattcagaag taatccctca ttcccgctgt ggcccacagc gggaacatcg
    28441 ttacctctga cacatagatt ttacccgccc cctctcctat tcttttcagt atgatctcag
    28501 gaggttgcta tgtgtggacg ctttgcccaa tcccaaacgc gtgaggatta ccttgcgctt
    28561 ctcgcggaag atattgaacg cgatattccc tacgatcccg aacccattgg cagatacaat
    28621 gtcgcgccgg gaaccaaagt tcttctgctc agtgaacgtg atgaacacct tcatctggat
    28681 ccggttttct ggggatatgc tcccggatgg tgggataaac cgccgctgat taacgcccgc
    28741 gtagaaactg cctcaaccag tcgcatgttt aaaccgctct ggcaacatgg tcgggcaatc
    28801 tgttttgccg atggctggtt tgagtggaaa aaagaaggcg acaaaaaaag ccttatttta
    28861 tctatcgcgc tgacggacaa cctgttttta tggccgcgat aggtagcata ccgtttgagc
    28921 gtggtgacga agccgaagga tttttgatcg tcactgcggc ggcagatcaa ggtctggtag
    28981 atattcatga ccgccgcccg ctggttctgt cgccagaagc tgcacgggaa tggatgcggc
    29041 aagacattgg tggtaaagaa gcctcagaaa tcgctaccag aagctgtgtg ccagcaaacc
    29101 agttcacctg gcaccctgtg tcgcgcacgg tgggtaatgt caaaaaccag ggcgcggagt
    29161 taattcaacc tgtttgttga catcctccac gccctgaatg acgaggaccc ctgctacgtt
    29221 caggctgtcg cctgaatcat ttcggtgggt tcctgcttca acgagcggcc tgactgcacc
    29281 atctctccac aggcaagaac ggcgtgcccc gccgctaaaa tgttacgagc tccatttaca
    29341 tcggcgttcg ctgtatatcc acatacctgg catctgaatt tactttgtga caggcg
//
LOCUS       DAZPAB010000030        29283 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZPAB010000030 DAZPAB010000000
VERSION     DAZPAB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29283
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.030"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="ACHUPG_001197"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="ACHUPG_001197"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR0860885.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="ACHUPG_001198"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="ACHUPG_001198"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR0860886.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACHUPG_001199"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="ACHUPG_001199"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR0860887.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACHUPG_001200"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="ACHUPG_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR0860888.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACHUPG_001201"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="ACHUPG_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR0860889.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACHUPG_001202"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="ACHUPG_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR0860890.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="ACHUPG_001203"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="ACHUPG_001203"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR0860891.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="ACHUPG_001204"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="ACHUPG_001204"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR0860892.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="ACHUPG_001205"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="ACHUPG_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR0860893.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACHUPG_001206"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="ACHUPG_001206"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR0860894.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACHUPG_001207"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="ACHUPG_001207"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR0860895.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="ACHUPG_001208"
     CDS             8212..9075
                     /locus_tag="ACHUPG_001208"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR0860896.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="ACHUPG_001209"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="ACHUPG_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR0860897.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="ACHUPG_001210"
     CDS             10411..11028
                     /locus_tag="ACHUPG_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR0860898.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="ACHUPG_001211"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="ACHUPG_001211"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR0860899.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="ACHUPG_001212"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="ACHUPG_001212"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR0860900.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="ACHUPG_001213"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="ACHUPG_001213"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR0860901.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="ACHUPG_001214"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="ACHUPG_001214"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR0860902.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="ACHUPG_001215"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="ACHUPG_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR0860903.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="ACHUPG_001216"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="ACHUPG_001216"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR0860904.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="ACHUPG_001217"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="ACHUPG_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR0860905.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="ACHUPG_001218"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="ACHUPG_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR0860906.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="ACHUPG_001219"
     CDS             21859..23568
                     /locus_tag="ACHUPG_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR0860907.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="ACHUPG_001220"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="ACHUPG_001220"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR0860908.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="ACHUPG_001221"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="ACHUPG_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="ACHUPG_001222"
     tRNA            27625..27719
                     /locus_tag="ACHUPG_001222"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            27991..29202
                     /locus_tag="ACHUPG_001223"
     CDS             27991..29202
                     /locus_tag="ACHUPG_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0860909.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
BASE COUNT         7235 a         7841 c         7349 g         6858 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    29101 atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg
    29161 tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc
    29221 tgatgttttc ctcaattaag tattttatcc tctggcctac aaggctggag gatttttgtt
    29281 att
//
LOCUS       DAZPAB010000031        28914 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZPAB010000031 DAZPAB010000000
VERSION     DAZPAB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.031"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="ACHUPG_001224"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="ACHUPG_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR0860910.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="ACHUPG_001225"
     CDS             complement(2138..2584)
                     /locus_tag="ACHUPG_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR0860911.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="ACHUPG_001226"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="ACHUPG_001226"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR0860912.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="ACHUPG_001227"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="ACHUPG_001227"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR0860913.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="ACHUPG_001228"
     CDS             complement(5347..6009)
                     /locus_tag="ACHUPG_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR0860914.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="ACHUPG_001229"
     CDS             6182..6595
                     /locus_tag="ACHUPG_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR0860915.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="ACHUPG_001230"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="ACHUPG_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR0860916.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="ACHUPG_001231"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="ACHUPG_001231"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFR0860917.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="ACHUPG_001232"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="ACHUPG_001232"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR0860918.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="ACHUPG_001233"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="ACHUPG_001233"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR0860919.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="ACHUPG_001234"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="ACHUPG_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR0860920.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="ACHUPG_001235"
     tRNA            complement(9861..9948)
                     /locus_tag="ACHUPG_001235"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="ACHUPG_001236"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="ACHUPG_001236"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR0860921.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="ACHUPG_001237"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="ACHUPG_001237"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR0860922.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="ACHUPG_001238"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="ACHUPG_001238"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFR0860923.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /gene="hyaD"
                     /locus_tag="ACHUPG_001239"
     CDS             14005..14592
                     /gene="hyaD"
                     /locus_tag="ACHUPG_001239"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR0860924.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="ACHUPG_001240"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="ACHUPG_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR0860925.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="ACHUPG_001241"
     CDS             14984..15841
                     /locus_tag="ACHUPG_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR0860926.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="ACHUPG_001242"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="ACHUPG_001242"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR0860927.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="ACHUPG_001243"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="ACHUPG_001243"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR0860928.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="ACHUPG_001244"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="ACHUPG_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR0860929.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="ACHUPG_001245"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="ACHUPG_001245"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR0860930.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="ACHUPG_001246"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="ACHUPG_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="ACHUPG_001247"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="ACHUPG_001247"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR0860931.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="ACHUPG_001248"
     CDS             complement(22884..24023)
                     /locus_tag="ACHUPG_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR0860932.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="ACHUPG_001249"
     CDS             complement(24069..26165)
                     /locus_tag="ACHUPG_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR0860933.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="ACHUPG_001250"
     CDS             complement(26165..26911)
                     /locus_tag="ACHUPG_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR0860934.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /gene="gfcB"
                     /locus_tag="ACHUPG_001251"
     CDS             complement(26908..27552)
                     /gene="gfcB"
                     /locus_tag="ACHUPG_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR0860935.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="ACHUPG_001252"
     CDS             complement(27659..27964)
                     /locus_tag="ACHUPG_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0860936.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="ACHUPG_001253"
     CDS             complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="ACHUPG_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR0860937.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7003 a         7320 c         7600 g         6991 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgtctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaattgtt
    28201 acgaaagcta ttaattattg ttggtaatat agtttcaagt ggtactataa ctggctgtta
    28261 aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg
    28321 gaaaagtaac tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag
    28381 tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc
    28441 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
    28501 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
    28561 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
    28621 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
    28681 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa
    28741 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
    28801 tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtgg tttctgtatt
    28861 aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta ataa
//
LOCUS       DAZPAB010000032        28238 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZPAB010000032 DAZPAB010000000
VERSION     DAZPAB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.032"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..1164
                     /locus_tag="ACHUPG_001254"
     CDS             <1..1164
                     /locus_tag="ACHUPG_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR0860938.1"
                     /translation="KPIWNWEISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTR
                     PTYFGDTDGSVGAVLYALFATGHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLD
                     TNVNTIISILNRRDNVLESLQPYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLL
                     KRIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIM
                     ANHHGITFDDQRKRYIIGPITVSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRA
                     ISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNA
                     RDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            1354..1467
                     /gene="yhiY"
                     /locus_tag="ACHUPG_001255"
     CDS             1354..1467
                     /gene="yhiY"
                     /locus_tag="ACHUPG_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR0860939.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1530..2597
                     /locus_tag="ACHUPG_001256"
     CDS             1530..2597
                     /locus_tag="ACHUPG_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR0860940.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2594..5329
                     /gene="rbbA"
                     /locus_tag="ACHUPG_001257"
     CDS             2594..5329
                     /gene="rbbA"
                     /locus_tag="ACHUPG_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR0860941.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIQARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5329..6453
                     /locus_tag="ACHUPG_001258"
     CDS             5329..6453
                     /locus_tag="ACHUPG_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0860942.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6512..6787
                     /locus_tag="ACHUPG_001259"
     CDS             6512..6787
                     /locus_tag="ACHUPG_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFR0860943.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            6784..7143
                     /locus_tag="ACHUPG_001260"
     CDS             6784..7143
                     /locus_tag="ACHUPG_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR0860944.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(7263..7664)
                     /gene="nikR"
                     /locus_tag="ACHUPG_001261"
     CDS             complement(7263..7664)
                     /gene="nikR"
                     /locus_tag="ACHUPG_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR0860945.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7670..8476)
                     /gene="nikE"
                     /locus_tag="ACHUPG_001262"
     CDS             complement(7670..8476)
                     /gene="nikE"
                     /locus_tag="ACHUPG_001262"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR0860946.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            complement(8473..9237)
                     /gene="nikD"
                     /locus_tag="ACHUPG_001263"
     CDS             complement(8473..9237)
                     /gene="nikD"
                     /locus_tag="ACHUPG_001263"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR0860947.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(9237..10070)
                     /gene="nikC"
                     /locus_tag="ACHUPG_001264"
     CDS             complement(9237..10070)
                     /gene="nikC"
                     /locus_tag="ACHUPG_001264"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR0860948.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(10067..11011)
                     /gene="nikB"
                     /locus_tag="ACHUPG_001265"
     CDS             complement(10067..11011)
                     /gene="nikB"
                     /locus_tag="ACHUPG_001265"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR0860949.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(11011..12585)
                     /gene="nikA"
                     /locus_tag="ACHUPG_001266"
     CDS             complement(11011..12585)
                     /gene="nikA"
                     /locus_tag="ACHUPG_001266"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0860950.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12696..13283)
                     /gene="acpT"
                     /locus_tag="ACHUPG_001267"
     CDS             complement(12696..13283)
                     /gene="acpT"
                     /locus_tag="ACHUPG_001267"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR0860951.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(13338..14387)
                     /locus_tag="ACHUPG_001268"
     CDS             complement(13338..14387)
                     /locus_tag="ACHUPG_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR0860952.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            14521..15738
                     /locus_tag="ACHUPG_001269"
     CDS             14521..15738
                     /locus_tag="ACHUPG_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0860953.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15742..16299)
                     /gene="dcrB"
                     /locus_tag="ACHUPG_001270"
     CDS             complement(15742..16299)
                     /gene="dcrB"
                     /locus_tag="ACHUPG_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR0860954.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(16372..17037)
                     /locus_tag="ACHUPG_001271"
     CDS             complement(16372..17037)
                     /locus_tag="ACHUPG_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR0860955.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            17258..17503
                     /gene="tusA"
                     /locus_tag="ACHUPG_001272"
     CDS             17258..17503
                     /gene="tusA"
                     /locus_tag="ACHUPG_001272"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR0860956.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(17605..19803)
                     /gene="zntA"
                     /locus_tag="ACHUPG_001273"
     CDS             complement(17605..19803)
                     /gene="zntA"
                     /locus_tag="ACHUPG_001273"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR0860957.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19877..20503)
                     /locus_tag="ACHUPG_001274"
     CDS             complement(19877..20503)
                     /locus_tag="ACHUPG_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR0860958.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            20644..21003
                     /locus_tag="ACHUPG_001275"
     CDS             20644..21003
                     /locus_tag="ACHUPG_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR0860959.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(21006..21275)
                     /locus_tag="ACHUPG_001276"
     CDS             complement(21006..21275)
                     /locus_tag="ACHUPG_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR0860960.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(21265..21861)
                     /gene="rsmD"
                     /locus_tag="ACHUPG_001277"
     CDS             complement(21265..21861)
                     /gene="rsmD"
                     /locus_tag="ACHUPG_001277"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR0860961.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            22011..23507
                     /gene="ftsY"
                     /locus_tag="ACHUPG_001278"
     CDS             22011..23507
                     /gene="ftsY"
                     /locus_tag="ACHUPG_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR0860962.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            23510..24178
                     /gene="ftsE"
                     /locus_tag="ACHUPG_001279"
     CDS             23510..24178
                     /gene="ftsE"
                     /locus_tag="ACHUPG_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR0860963.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            24171..25229
                     /gene="ftsX"
                     /locus_tag="ACHUPG_001280"
     CDS             24171..25229
                     /gene="ftsX"
                     /locus_tag="ACHUPG_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR0860964.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            25474..26328
                     /gene="rpoH"
                     /locus_tag="ACHUPG_001281"
     CDS             25474..26328
                     /gene="rpoH"
                     /locus_tag="ACHUPG_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR0860965.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            26599..27702
                     /gene="livJ"
                     /locus_tag="ACHUPG_001282"
     CDS             26599..27702
                     /gene="livJ"
                     /locus_tag="ACHUPG_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR0860966.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(27901..28236)
                     /gene="panM"
                     /locus_tag="ACHUPG_001283"
     CDS             complement(27901..28236)
                     /gene="panM"
                     /locus_tag="ACHUPG_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR0860967.1"
                     /translation="MDLQKIWPEYSPSSLQVDDNHRIYAARFNERLLAAVRVTLSGTE
                     GALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGVEDRGVMTAFMQALGFT
                     AQQGGWEKR"
BASE COUNT         6737 a         7298 c         7796 g         6407 t
ORIGIN      
        1 aaacccatct ggaactggga aatatcgcaa tacaatgtat ttgatattaa attcgaaaca
       61 attataaaac cccagttcgc tgacagaacc tatttctcca attgttcacc tgttgatccc
      121 acaagtacca ggccgaccta ttttggcgat accgatggct cagtcggtgc agttttgtac
      181 gcgctttttg caacaggaca tttgggaatc atggctgaag gagaaaattt ccttagccag
      241 ttgcttaata tagaagatga agttctcaac gtattgctaa gggagaactt taatgagcaa
      301 cttgacacta atgttaatac aataatcagt atactgaaca gaagagataa tgttttagaa
      361 agtcttcagc cttatttggt tattaataaa gacgcagtca cgccatgtac gtttcttggc
      421 gaccaaaccg gcgatcgatt tagtaatatc tgtggcgatc aatttatcat cgatttgtta
      481 aaacgcataa tgagtatcaa tgaaaatgtt catgtattag ctggaaatca tgaaacgaat
      541 tgcaatggta actatatgca aaacttcacg cgcatgaaac cacttgatga ggatacctac
      601 gacgggataa aagattatcc tgtatgtttc tatgacccca aatacaaaat aatggcaaat
      661 catcatggga taacttttga tgatcagcga aaacgttaca ttataggacc gataactgtc
      721 tctattgatg aaatgaccaa tgctctcgat ccggtcgaat tagcggaaat tattaataaa
      781 aagcatcatg ctataattaa tggcaaaaag tttaaaacta gccgggctat ctcttgtcga
      841 tcctttaatc gttatttttc tgtttcgaca gattacagac ctaaacttga agcactatta
      901 gcatgttcac aaatgttagg tataaatcaa gttgttgcac ataacggaaa tggtgggaga
      961 gaacgcatag gtgaaacggg gacagtttta ggtcttaacg cccgtgacag taaacatgct
     1021 ggaagaatgt tcagcatgca taattgccaa attaaccctg gtgcagggcc agagataacc
     1081 actccctgga agtcttacca gcatgaaaaa aacagaaatg gacttatgcc tttgattaga
     1141 aggcgcacaa tgttacagct ctgaaccata gacaacatga atattaatgt tactatctgg
     1201 ttgagactgt tgataaaacg taaaaaggaa tgctctgttc ctgcaactga acaggcgtga
     1261 aatgcgatcc aacaggatgg ataccgtttt gtcagccagt tttatagatc acacttatga
     1321 gtatgcgtgt ttcatttacg gccaggaatg ctgatgatga caacattact acccgttttc
     1381 actaagcctt ccccgttggc gctcaatgct ctgcgcgctg gtcgtatttg tcgtttcctt
     1441 cttatcccgg atggaagaat ccgctgaacg tcgttcggcg gcagttgtaa cacgcgtcgt
     1501 ttgcttacag ggattatggc gggtcgatta tggataagag taagcgccat ctggcgtggt
     1561 gggttgtcgg ggcactggcg gtggctgccg tcgtggcgtg gtggctgttg cgcccggcgg
     1621 gtgtgccgga aggctttgct gtcagcaatg ggcgcattga agcgacggaa gtggatatcg
     1681 ccagcaaaat tgccgggcgt atcgacacca ttctggtgaa agaaggccag tttgttcgcg
     1741 aaggcgaagt gctggcgaag atggatactc gcgtgttgca ggaacagcga ctggaagcca
     1801 tcgcgcaaat caaagaggca caaagcgccg ttgccgccgc gcaggctttg ctggagcaac
     1861 gacaaagtga aactcgtgcc gcacagtcgc tggtcaatca acgccaggcc gaactggact
     1921 ctgttgccaa acgtcatacg cgttcccgct cgctcgccca acgaggggct atttctgcgc
     1981 aacagctgga tgacgaccgc gccgccgctg agagcgcccg agctgcgctg gaatcagcga
     2041 aagcccaggt ttcggcttct aaagcggcta tagaagcagc acgcaccaat atcattcagg
     2101 cgcaaactcg ggtcgaagca gcgcaagcca ctgaacggcg cattgccgca gatatcgatg
     2161 acagtgaact gaaagccccg cgtgacggac gcgtgcagta tcgggttgcc gagccagggg
     2221 aagtgctggc ggcaggcggt cgggtgctga atatggtcga tctcagcgac gtctatatga
     2281 ccttcttcct gccaaccgaa caggcgggca cgctgaaact gggcggtgaa gcccggctga
     2341 tcctcgatgc cgcgccagat ctgcgtattc ctgcaaccat cagttttgtc gccagtgtcg
     2401 cccagttcac gccaaaaacc gtcgaaacca gcgatgaacg gctgaaactg atgttccgcg
     2461 tcaaagcgcg tatcccaccg gaattactcc agcagcatct ggaatatgtc aaaaccggtt
     2521 taccgggcgt agcgtgggtg cgggtgaatg aagaacttcc gtggcctgac gacctcgtgg
     2581 tgaggttgcc gcaatgacgc atctggaact ggttcccgtc ccgcctgtcg cgcaactggc
     2641 gggcgtgagc cagcattatg gaaaaaccgt tgcgctgaac aatatcactc tcgatattca
     2701 ggcccgttgc atggtcgggc tgattggtcc ggatggcgtc gggaagtcga gtttattgtc
     2761 actgatttcc ggtgcccgcg tcattgagca gggcaatgtg gtggtgctgg gtggcgatat
     2821 gcacgacccg aagcatcgcc gagacgtctg cccgcgcatc gcctggatgc cgcaggggct
     2881 gggcaaaaac ctctaccaca ccttgtcggt gtatgaaaat gtcgattttt tcgctcgcct
     2941 gttcggtcac gataaagcgg agcgggaagc gcgaattaat gagctgctga ccagcaccgg
     3001 gttagcaccg tttcgcgatc gtccggcagg taaactctcc ggcgggatga agcaaaaact
     3061 tgggctgtgc tgcgcgttaa tccacgaccc ggaactgttg atccttgatg agccaacaac
     3121 gggggttgac ccgctctccc gcgcccagtt ctgggatctg atcgacagta ttcgccagcg
     3181 gcagagcaat atgagcgtgc tggtcgccac cgcctatatg gaagaggccg aacgcttcga
     3241 ctggctggta gcgatgaatg ccggagaagt gctggcaacc ggcagcgccg aagagctgcg
     3301 gcagcaaacg caaagcgcca cgctggaaga agcatttatt aatctgttac cgcaagcgca
     3361 acgccaggcg catcaggcgg tagtgatccc accgtatcaa cctgaaaacg cagagattgc
     3421 catcgaagcg cgcgatctga ccatgcgttt tggttccttc gttgccgttg atcacgtcaa
     3481 tttccgcatt ccacgcgggg agatttttgg attccttggt tcgaacggct gcggtaaatc
     3541 caccaccatg aaaatgctta ccggactgct gcccgccagc gaaggtgagg cgtggctatt
     3601 tgggcaaccg gttgatccaa aagatatcga tacccgccgt cgtgtgggct atatgtcgca
     3661 ggcgttttcg ctctataacg aactcaccgt gcggcaaaac ctggagttac atgcccgttt
     3721 gtttcacatc ccggaagcgg aaattcccgc gcgagtggct gaaatgagcg agcgttttaa
     3781 gctcaacgac gttgaagatg ttctgccgga gtcattgccg ctcggcattc gccagcggct
     3841 ttcgctggcg gtggcggtga ttcatcgccc ggagatgtta atcctcgatg agcctacttc
     3901 tggtgtcgat ccggtggcga gggatatgtt ctggcagttg atggtcgatc tctcgcgcca
     3961 ggacaaagtg actatcttca tctccaccca ctttatgaac gaagcggaac gttgcgaccg
     4021 catctcactg atgcacgccg gaaaagtgct tgccagcggt acaccgcagg aactggttga
     4081 gaaacgcgga gccgccagtc tggaagaggc atttatcgcc tatttgcagg aagcggcagg
     4141 gcagagcaac gaagccgaag cgccgcccgt ggtacacgac accacccacg cgccgcgtca
     4201 gggatttagc ctgcgccgtc tgtttagcta cagccgccgc gaagcgctgg aactgcgacg
     4261 cgatccagta cgttcgacgc tggcgctgat gggaacggtg atcctgatgc tgataatggg
     4321 ttacggcatc agtatggatg tggaaaacct gcgctttgcg gtgctcgacc gcgaccagac
     4381 cgtcagtagc caggcgtgga cactcaacct ctccggttcc cgttacttta tcgaacagcc
     4441 gccgctcacc agttatgacg agcttgatcg tcggatgcgt gcgggcgata tcacggtggc
     4501 cattgagatc ccgcccaatt tcgggcgcga tatcgcgcgt ggtacgcctg tggaactcgg
     4561 cgtctggatc gacggagcga tgcccagtcg tgccgaaacg gtaaaaggtt acgtgcaggc
     4621 aatgcaccag agttggcttc aggatgtggc gagccgacaa tcgacacccg ccagccaaag
     4681 cgggctgatg aacattgaga cgcgttatcg ctataacccg gacgtaaaaa gcctgccagc
     4741 gattgttccg gcggtgatcc cgcttctgct gatgatgatc ccgtcaatgc taagcgccct
     4801 tagcgtggtg cgggaaaaag agctagggtc gattatcaac ctttacgtga cccccaccac
     4861 ccgtagcgaa tttttgcttg gcaaacagct gccgtacatc gcgctgggga tgctgaactt
     4921 tttcctgctc tgcggcctgt cggtgtttgt gtttggcgta ccgcataaag gcagtttcct
     4981 gacgctcacc ttggcggcgc tgctgtatat catcattgcc actggaatgg ggctgctgat
     5041 ctccaccttt atgaaaagcc agattgccgc cattttcgga acggcgatta tcacattgat
     5101 cccggcgaca cagttttccg ggatgatcga tccggtagct tcgctggaag ggcctggacg
     5161 ttggatcggc gaggtttacc cgaccagtca ttttctgact atcgcccgcg gaacgttctc
     5221 gaaagcgctg gatctgactg atttgtggca actttttatc ccgttgctga tagccatccc
     5281 gctggtgatg ggcttaagca tcctgctgct gaaaaaacag gagggatgat gcgccattta
     5341 cgcaatattt ttaatctggg tatcaaagag ttgcgcagtc tgctcggtga taaagcgatg
     5401 ctgacgctga ttgtcttctc gtttacggtg tcggtgtatt cgtcagcgac cgttacgcca
     5461 ggatcgttga acctcgcgcc gatcgccatt gccgatatgg atcaatcgca gttatcgaac
     5521 cggatcgtta acagcttcta tcgtccgtgg tttttgccac cggagatgat caccgccgat
     5581 gagatggatg ccggactgga cgccggacgc tataccttcg cgataaatat tccgcctaat
     5641 tttcagcgtg atgtcctcgc cggacgccag ccggatattc aggtgaacgt cgatgccacg
     5701 cgcatgagcc aggcatttac cggcaatggg tatatccaga atattatcaa cggtgaagtg
     5761 aacagctttg tcgcgcgcta ccgtgataac agcgaaccgt tggtatcgct ggaaacccgg
     5821 atgcgcttta acccgaacct tgatcccgcg tggtttggcg gggtgatggc gatcatcaac
     5881 aacattacca tgctggcgat tgtattgacc ggatcggcgc tgatccgcga gcgtgaacac
     5941 ggcacggtgg aacatttact ggtgatgccg ataacgccgt ttgagatcat gatggcgaag
     6001 atctggtcga tggggctggt ggtgctggtg gtatcgggat tatcgctggt gctgatggtg
     6061 aaaggtgtac tgggcgtacc gattgaaggc tcgatcccgc tgtttatgct gggcgtggcg
     6121 ctcagtctgt ttgccaccac gtcaatcggc atttttatgg ggacgatagc gcgttcaatg
     6181 ccgcaactgg ggctgctggt gattctggtg ctgctgccgc tgcaaatgct ttccggtgga
     6241 tccacgccgc gcgaaagtat gccgcagatg gtgcaggaca ttatgctgac catgccgacg
     6301 acacactttg ttagcctcgc gcaggccatc ctctaccggg gtgccggatt cgaaatcgtc
     6361 tggccgcagt tcctgacgct gatggcaatt ggcggcgcgt ttttcaccat cgcactgctg
     6421 cgattcagga agacgattgg gacaatggcg taaggaaggg aaacttgact tcatactgtg
     6481 atgtcataaa gtgaagtcat aaggacatgc catgagaacc aaggtacagg ctttacggaa
     6541 gaaacaaaaa aatacattgg atcaaatatt taaaactcct gttcctcaag ggatcaagtg
     6601 gtctgatata gagtcactgg ttaaagcatt aggcggagaa attaaggaag gaagaggttc
     6661 gcgttgcaag ttcatactaa atatgagcgt tgcgtgtttc catcggcctc atccgtcgcc
     6721 agataccgat aaaggcgctg tagaaagcgt gtgtgactgg ttactaagta taggggtaaa
     6781 accatgatca aactgaagac gccaaactct atggagattg ccgggcaacc agctgttatt
     6841 acctacgtgc cagaactgaa tgcatttcgt ggcaaattcc tgggcttgtc aggttattgt
     6901 gattttgtct ctgacagtat tcaggggctg caaaaagaag gagagctttc cctgcgagag
     6961 tatcttgagg attgtaaagc tgcgggtatt gagccgtatg cgcgtacaga aaagattaag
     7021 acctttacgt tacgctatcc agaatcgctg agtgaacgcc ttaataatgc ggctgcgcag
     7081 caacaagtct ctgttaatac ctatattatt gaaacactga atgagcgttt gaatcattta
     7141 taaattttag cgacgtacat tacgcagaat atctgccttt acaggatcta tctatgcaac
     7201 tgaacttaat tttataaatt aagttcagtt aatatctttt ctttccttgt tttagaaata
     7261 actcaatctt ccttcggcag gcactgcaaa tgcccgtgcc gcacgccgcg ctgggcgata
     7321 acgtcatcgg caaaatgctg cacgtcaccc atgtcacctt tcaacacggc gatttccaga
     7381 cagtcgtcgt ggttgatgtg cacatgtagc gtggcgacgg agaggtcgtg gtgatgatgc
     7441 tgggtggaaa caatacggct ggctaagtcg cgtttttcgt gttcatacac atacgacagc
     7501 accgcgaaac cttgtgtgcc gtgctgctgg gtagcttcct gcgccagggc actacgcaga
     7561 atatcgcgga tagcttcgga acggttgtta taaccacgac gctggctcag gctgtccagc
     7621 gtctccagta aatcgtcatc aagcgtgatg gtgactcgtt gcattggcgt taaacctttt
     7681 ctgaggtgcg acggcgcacg gggaatgcgg gtaataccgc gttttgtagc acacgtccgg
     7741 cgtcagagga aaaggttaat ttatctccca ccgcctgggt ttcggcgatt tgtccgttgt
     7801 ccatcaccat cactcgctgg caaaagcgtt ccaccaggcg taagtcatga gtgatgaaca
     7861 ggcaggcagt gccaaactgt tgttgtagct ttttcagcag gcgaatgacg cccgcctgta
     7921 acacgagatc aaggttagaa acggcttcat ccagaatcag caatttcggt tcgaccgcca
     7981 gcgcgcgtgc caggcagacg agctggagct ggccgccgct taactgcggt gggcgtttgt
     8041 cgagaacgct gtcatcgaga tcgaccgcct tcagcatttc gctggcgcgc gccagttgtt
     8101 cggatttttt cagtgatagc aggtggcgca tcggttcacg caggatctcg cgcacggttt
     8161 tgcgcggatt cacggcactg atggagtcct gaaacaccat ctgaatatca cggcggaagg
     8221 ctttacgttg ggcgcgatta agtttcgcca gcgattcgcc gtgccagcta atattccctt
     8281 gtgagggaga ctctaagcct accagcagcc gcgcgagggt gctttttccg cagccgctgc
     8341 gtcccagcag ggcgacggtt tcgccgcttt tcagggtcag ggaaacgtta ttcagcaccg
     8401 cctgatgttg atgttttccg ctaaatccac cgtgggcata gtgatgggaa aggccgcaga
     8461 cgttaagtaa ggtcatgatg ccagctccat accgtagagg gcgagatgag cggaaaccag
     8521 gctgcgcgtc accgtatgtt tgggggcgtt aaacagcgtt tctacatcgc cctgttcgac
     8581 aattttacct tgtgacatca ccgccacgtc atccgccaga cgcgccacaa cgcccatatc
     8641 atgggtgacc agcagcattc ccggcgcttg tttttgcata atgctttcca gcagatcgag
     8701 gatgcgcgcc tgtgctacca cgtcgaggtc ggtggtcggt tcatcggcga tgataaacgg
     8761 tgattcacac agcaccgcca tcgcaatcat catgcgctgc aacatgccgc cgctcatctc
     8821 gaacgggtac agcttcagca cgcgcgcggc gttttccagc cccaccgctt ctatggcagc
     8881 ggtaagcgta gcgtcatcgg cgggtttccc taacgccagg caggtttccc gcgcgtgggt
     8941 gtgcatggtg tgcagtggat taaaggcgct gcgcgggttc tgcatgatgg tggcaatttt
     9001 gatgccgcgc agggcgtaag gcgaaaccgg tttgccatcg gctaaaattt cccccgccgt
     9061 ctggcgaacg ccagcgggca aaatgcccag cgtcgcggcg caggttaatg atttcccgct
     9121 gccgctaccg ccgactaacg ccagcacgcg cccgcgttgc agggttaacg atacaccgtg
     9181 taccagcggc tgcgcggcct gtagcgtgat attacgtagt tcaatctgtt gcggcattag
     9241 tgtgcgtgct ccgtcaccag atgaggatcc agatgatcgc gcagtgcgtc acccaccagg
     9301 ttaaaggcca tcacgctgat aaacagcgcc agccccggcc agaacatttg cagcggctgg
     9361 gtccagatat actggcgcgc gtcgttaatc atcacgcccc attcggcggt cggcgcggtc
     9421 acaccgaggc caaggaaaga catccccgcg acgtgcagca tcatatggcc gatatccagc
     9481 gttgccagca ccagcagcga agggatcacc gcgcctgcca gatgatcgac aaacacccgc
     9541 acatggcccg caccggaaag ccgtgacgcc agcacaaact cgcgttggcg tagtgaaatc
     9601 accaagctgc gcaccatgcg tgcataccac gcccagtgcg acagggcgat ggcgataatt
     9661 acgttggtca gcccggtgcc gagcacgcca accataaaga acgacagaat cgaggtcggg
     9721 aaggtcataa acatatcggc gacgcgcatg gtggcctgat caacgtgccc gccaatcaac
     9781 ccggcgctgc cgccaataac cagccctaat gtcagcacca gcagcaggca ggccattacc
     9841 gaaccgagcg acacgcgggt cgctgccatc agccgcgaga aaatatcgcg acctaagtga
     9901 tcggtgccca gccagtgctg cgcatccggc gaaagcaggc acgacggcaa atcaattgcc
     9961 tgtggatcgt acggctgcca ccactggctg gtgagcgcaa tcagcgccag cagggcgata
    10021 atgatcagcg ccaggcgtac cgaccagcgg gaagagagga aaaagttcac gcgtgcgctc
    10081 cttcatgacg acgaatgcgc gggtccagcg cggcgttgag caaatcgaca atcaaattac
    10141 agaccacaaa aaccaccacc atcatcagcg taaagcactg aatcaccgga tagtcacggt
    10201 taaaaatcgc cgacaccgca tagcgcccga cgccaggcca ggcaaagatg ttttcgataa
    10261 tcatcgtccc gccaatcaat tcgccaatgt gcatccccac ggcggtgatc atcggcagcg
    10321 aggcattgcg cagaatatgg cggcgttcgg tctgtttgtc attcaggccg cgtagccgcg
    10381 cccaggtgac gtgacgctga ccggcgacgt ccagcatact ggcgcgcagc agtcgcgcgt
    10441 taatcgccag cgacataaag gcgatggaaa ccgcaggtaa aatgatgtgc tgccagctgc
    10501 cgtagcccat cgcgggtagc cattgcagat acaccgaaaa cgccatcact agcaaaaatg
    10561 ccagccagaa gttgggcatc gacacgccaa gaaacgcgat aaagcgcacg gcgaaatccg
    10621 gcaaacgatc gcggtggcgc gccgcccaga taccgagcgg tacggaagtg agcagaatta
    10681 ataccagcgc cgcacctgca agttccagcg tggcgggcag gaagttcagc atatcgtcca
    10741 gtaccgggcg ttggctggcg aatgagatac caaagtcaag atgcagcgcc ttccacaacc
    10801 aggtaccgta ctggacgtac agcggctgat ccagcccgag catggtgcgg gtagaggcca
    10861 gcatctccgg cgtcggcggc aggttagata aacgcagata atcgagcgcc gggtcgccgg
    10921 taccgaggcg cagcatcaga aaaatgatca ccgaggcggc gagcaccatc gggatcagca
    10981 gcagaaagcg gcgtaatacg taacgcaaca ttaaggtttc accggtttaa tctgttcgaa
    11041 cggaatttcg gtggcgatcg gcgcgtaggg gatattaccc agctccggtt ttgataccac
    11101 catcatcgag atgtagctga taggcagata aaccgcctcg tcatgcagac gggtcagaat
    11161 gtcgcgatac agcgcctgac gttgcgtttc gtcatgggtc gccagcactt cgccgatctc
    11221 tttatcaatc agcggcttgt cggctaatcc ttgctgtgcc tggaagtcag cgtgcgacgg
    11281 tacgcgcatt gaactcagga aggcgtgtgg atcgtatggc gcgccccagg tgcggtggaa
    11341 aatcatgcca aaacgaccgt cgcgctgacg agcatagata ctgctctctt cttcgccaat
    11401 cagcgagaca tctgcgccaa tctggcgcat atcagcctga atgatttccg ccatcgattt
    11461 gcttaacgca tcggtgccga tgaacgacag ttcaatgcgc agcggctgac catttttctc
    11521 gcggatgtct ttgcccgcag gcagcgtcca accggctttt tccagcaact ctttcgcttt
    11581 ctgcggatcg tactggcgcg gtttcaggcc gagattagcg taaggcacag aaggggcaaa
    11641 cagggtgtcg gcgacctgct gggtgccata taacgcgtta tcaatcagcg attttttgtt
    11701 taccgcgtaa ttaagagctt cacgtactgc cagctcgttg gtgggggctt tggcggtatt
    11761 gagcgccagc atcacggttt cgatcggctg tgacagttgg gtgtggtaag ccggattctg
    11821 gctaaagcgg gcgaaggtat cgagcggtaa taacccttcg tttccgtaca gcaggtcgat
    11881 atcgccagtt tcaaacgcca ccgcgcgggt agtcgggtcc gggatgacgt taaaggtgat
    11941 ctttttaatc gctggctttt cgccccagta gttttcgtta cggacgaaga catcgtactg
    12001 atttagtttc gattcctgca aaatccacgg tccggtgcca atcggcgctt taattccgtt
    12061 catggtttca tggtttttaa actgcgaggg agcgataaaa cggaaaggac ggggcagggc
    12121 cagttcttgc aggaaaggat agtaggcgct tttcagggta atttgcagct ctgttttatt
    12181 gagtgcttta acatcaacaa tctggtttgc cagctccagc caggcgtgac gttgacggtt
    12241 atcgagcact gcgcggaagt tttctgccgc cgcctcggca tcgaacggtt caccgttgga
    12301 gaatttcacg tcatcacgca gggtgaaggt ccaggtttta ccatcttctg aatgagtcca
    12361 gctttttgcc agccacggga tcaccgaacc gtctgcctga tatttcacca atggttcata
    12421 aaccatgctc tgggcgaaca tctggttagg cgtgtaaagg tgcgggttta gtggcccgac
    12481 attcaccggc caggcggtgg tgatttcatc tggtgcagcg gcatggacga taaaagacgc
    12541 acaagccagc agcgcaaata gagtgcggcg gagtgtggag agcatggtaa ccccaatgga
    12601 ttaaaataga tggcggaaat aagtatgacg attttaagta ttcgtcatac tgattacctg
    12661 ttctggatca atagatgggc agtcgggtgg gcggatcagt taactgaatc gatccattgc
    12721 acactgtcgg cggtgagcgt aaagggggtg ggggtgcaga tcgcaaggct taaattttct
    12781 agctgacaat ggctgactga cagcgaggag tgataggtgc tatctacgct gacgatttgc
    12841 caggcgctgc cgccacgctg tttaacgatg gcttctttgc gcgtccagat gcgccagaac
    12901 atttccagtt gctgatcagg atgcacggcg tccatttcag cgtgttcccc gaggctgaac
    12961 acggcgttcg ccagccagcg ccagttgacg cgcgggcgaa tcacttcgat atcgcagccg
    13021 acttcgcctt catcgctcaa cagcagagcg atatcgtcac cgctatggct taagttgaac
    13081 catagcggcg tttccggcgc gaatgcaggt ttgccttgtt cgccatagat gatctccggt
    13141 agcggggaaa gcgtgtgcga aagcaatgta cgccccgcca gccagcgttc gcgtcgtgga
    13201 ccttgcggtg cttgctcgcg taaacccggt ggcagtggag ctgcgcttaa ggtcgaaact
    13261 ttccccagaa ctatccgata catatcaggg ccaacgttta atggaaaatg aaagtgcgta
    13321 tcgtatcact tgtcgcctca tcccggtaac cgacttttcg gtctgcccgg ccccagtaaa
    13381 atcgccagtt tgctgccacc tttcgtcgtt tccatccaga ttttacacac gctggttaat
    13441 ggcaccgaaa gtagcatccc taccgggccg agcagccatc cccagattaa caatgaaaga
    13501 aataccacca tggtggacat ccccaggcga tggcccatca tccgtggttc taaaatattg
    13561 ccgatgacca tatggaccac taaaaacaat gcgccgacca gaatacattc gtaaacacca
    13621 ttaaacagca gcacctgaat cattggcggt acggcggaaa ttaccgcgcc gatattgggc
    13681 acgtagttga gcaaaaacgc cagtactgcc cacatcagcg caaactgtac gcccattagc
    13741 gccagcccca gccagacgat gacacctgtc cataaactga gtagcgtctt taacgccaga
    13801 taatgcgaaa cgcctttaag tgcgcggtgt aatcctgcga tgtgaatctg tgggttattc
    13861 agcgcaaaac gcattttgta agggacgtgg cgcacttcaa acagcataaa aactacggtc
    13921 atcaccagca aaagcacgct cgccattgcc ccggaaagcc cggtcattag cgctgtggtg
    13981 aaggtaacca ctttttccga gtccatccgc tgcagcattc gctccggcga catatgcaaa
    14041 ttaagaaaag gcaacatctc ctgcaattta aaaagtttgc gcgtcagctc cttattaaac
    14101 ttcggcagca tagagataaa ttcgttaaac gatgccgcca gtacgccgac cagcgcggtt
    14161 agtgcgatca gcatcaccac cactacaatc gtaatggcaa cggggcgttg tactccccga
    14221 cgaataaacc aggtaacgag cgggttgagg acgatggcaa aaaacagcgc cagtaacagc
    14281 tgcacaatga tatctgccgc tgcgtgaatg cccgcgagga tcactaccag cgaggccaac
    14341 ttgagcagaa tgtgcatgcc cgttttatcg ggttgagggg tttccattgg ggcttccttg
    14401 tgactttttg tgttaagtgt agcgcgggag tcacgccagc aatattctga ttcttactgc
    14461 tgattttcac gtcagcacat ggtaaaaatg aaacactgtt gtaaaaatgt ggtgatcctc
    14521 atgcccgaac ccgtagccga acccgcgcta aacggattgc gcctgaattt gcgcattgtc
    14581 tccattgtca tgtttaactt cgccagctac ctcaccatcg ggttgccgct cgctgtatta
    14641 ccgggctatg tccatgatgt gatgggcttt agcgccttct gggcaggatt ggttatcagc
    14701 ctgcaatatt tcgccacctt gctgagccgc cctcatgccg gacgttacgc cgatttgctg
    14761 ggacccaaaa agattgtcgt cttcggttta tgcggctgct ttttgagcgg tctggggtat
    14821 ctgacggcag gattaaccgc cagtctgcct gtcatcagcc tgttattact ttgcctgggg
    14881 cgcgtcatcc ttgggattgg gcaaagtttt gccggaacgg gatcgaccct atggggcgtt
    14941 ggagtggttg gctcgctgca tatcgggcgg gtgatttcgt ggaacggcat tgtcacttac
    15001 ggggcgatgg cgatgggtgc gccgttaggc gtcgtgtttt atcactgggg cggcttgcag
    15061 gcgttagcgt taatcattat gggcgtggcg ctggtggcca ttttgttggc gatcccgcgt
    15121 ccgacggtaa aagccagtaa aggcaaaccg ctgccgtttc gcgcggtgct tgggcgcgtc
    15181 tggctgtacg gtatggcgct ggcactggct tccgccggat ttggcgtcat cgccaccttt
    15241 atcacgctgt tttatgacgc taaaggttgg gacggtgcgg ctttcgcgct gacgctgttt
    15301 agctgtgcgt ttgtcggtac gcgtttgtta ttccctaacg gcattaaccg tatcggtggc
    15361 ttaaacgtag cgatgatttg ctttagcgtt gagataatcg gcctgctact ggttggcgtg
    15421 gcgactatgc cgtggatggc gaaaatcggc gtcttactgg cgggggccgg gttttcgctg
    15481 gtgttcccgg cattgggtgt agtggcggta aaagcggttc cgcagcaaaa tcagggggcg
    15541 gcgctggcaa cttacaccgt atttatggat ttatcgcttg gcgtgactgg accactggct
    15601 gggctggtga tgagctgggc gggcgtaccg gtgatttatc tggcggcggc gggactggtc
    15661 gcaatcgcgt tattactgac gtggcgatta aaaaaacggc ctccggaaca cgtccctgag
    15721 gccgcctcat catcttaaaa cttactgaat aaccagcgta ttaatgatgt tttctgcggt
    15781 ggtttgcgct ttttgctgat cgtcagctgg cagcgtaatt tgcatggtca gcagttgatt
    15841 acccacgtta cccagaataa cggaagagta cgccgtctgg cctttcgcgg agataatact
    15901 gtctaactgc tgcattttgt gacctttcag ctcaatggct ttattggtta ccacttgcag
    15961 ctgcggatcg cggctacgtt gctgatcttc cagacgcttc gccaacaccg ccagatcttc
    16021 tttcggatca tcgcccatga tgacgatgac tgctttctgc ccggtggcgt cggaccagac
    16081 atgcatgtta ttggcctgcg ttcccagctt accgctctgg tcggtcatat ccgctggcag
    16141 cgagaaactt aacttgccat caagcaggtt gacgggattc ccggtagcgt tactttccgc
    16201 aaccgaaccc tgcgccgtag cgttagtgtc tttatcatca caggccgcaa gccccataac
    16261 cagcaggcca attccgacat atttaaccag attgcgcatt gacttcttcc tttcgataaa
    16321 cggccataac ggctcattca tccatcttat cacaactctg ataacgaacc tttaactcgc
    16381 ctgcaaagcg ttgatttcgg atttatctgc cagtcttttc aacagcatat tgagtaatac
    16441 gccatacatt ggcaggaaga aaacgatact gattaacact ttgaaacagt aatcgaccag
    16501 cgcgatttcc atccagtgtt cagccataaa ggcatccggg ctacgccaga aggcaatgaa
    16561 gaaaaaggcc agcgtgtcgc tgacgttacc gaacagtgtg gacgctgtcg gtgccagcca
    16621 ccagcggcga ctctgacgca ggcggttaaa aacgtgcacg tcgaggattt gccccagcgc
    16681 gtaggccatg aaactggcgg tggcgatacg ggcgacaaac aggttgaagt gggcgagtgc
    16741 gccgaatccc tgccaggaac ccatatagaa tagcgacgag atgacgtagg agattaataa
    16801 cgcagggatc attaccgcga agataatgcg tcgggccagc ggtgcgccaa aaatacgcac
    16861 ggtcaggtcg gtagcaagaa aaataaacgg aaagctaaac gcgccccagg tggtatggaa
    16921 accaaaaatg gagacgggaa gctgaaccag atagttactg gaggtgatca ccagaagatg
    16981 aaataacgat aaccagaaca acgccttata gcgttgagtt tgcgagaaaa cgttcatatt
    17041 gtaccttttt gattaaccat tggggtgagg gaacccaata cgtacgacac gtctgttacc
    17101 ttaacataat aacgtgctta cccttttttc gagccgccgc atgatactgc tttacgttga
    17161 caatgcaatg gttaattttc acgcaatcgt taacctggtt tgcttacgga ctcacagggc
    17221 gtaaactagc gccgtttttt taagtgatga gaagaaaatg accgatctct tttccagccc
    17281 tgaccacaca ctcgacgcgc ttggcctgcg ctgcccggaa ccggtgatga tggtgcgcaa
    17341 aaccgtgcgc aatatgcagc ctggcgaaac gttgctgatt atcgccgacg atccggccac
    17401 tacccgcgat attcctgggt tttgtacctt tatggaacac gaactggttg cgaaagagac
    17461 ggatggactg ccttatcgtt atttgattcg taaaggcggt tgataggggc tgattggctt
    17521 cgatgccgcc ttttcctcac cccctaaccc tctccccaga ggggcgaggg gaccgatcgc
    17581 gctcaatgtt gcgatcggtt tgccttatct cctgcgcaac aaccttaacg cattcgccgt
    17641 caccagcacc gtcgcacccg tatctgccag cactgccagc cacaacccgg tcatccctaa
    17701 aagcgtggtg acgaggaaga tccctttcag tcccagcgca atggtgatgt tctggcggat
    17761 attggcgtga gtggcgcgtg ccagttcaat catttgcacc aggccgcgca ggtggttatg
    17821 ggttaatgct gcgtcggcgg tttccagcgc cacgtctgtg ccgctaccca tagcaatccc
    17881 gatggcggca gctttcatcg ctggcgcgtc gttaatacca tcaccgacca tcgccagggg
    17941 cgcatgttga ttcagcttgg tcaccgcttt gactttatct tccggcaata ggcccgcttt
    18001 aaactccagc cccagctccc cggcaattgc cgccgctgcg cgtggattat cgccggtgag
    18061 gatcactcct ttaaccccca gcgcgttcag ttcactgatg gcagttgtag cgtcggcgcg
    18121 cagggtatcc tgcaatgcaa tgatacccag cacgtcatca ttacgtacta ccagcacaac
    18181 cgtttgcccg gcgctttcca gttcattaat caaaccagca aatgcatcag cgggatgttt
    18241 cccggcagcg catatcaaca cgcgctcacc attaacctgc gcttcaatgc cagacccgac
    18301 cagtgcccgc tgtgattcgg cggtgggaat ggcgagttca gcaacctgtg cttcgcgtac
    18361 gatggcttgc gccagtggat gcgtcgcgcc ttgctcgacc gccgccgcca gtgtcagcag
    18421 ttcagattca ctaatacccg ttgccggatg aatcgcggta acgcgcggtt taccgacggt
    18481 cagcgtaccg gttttatcaa acgccacttg ggtaacacga cctagttgtt ccagcgccgc
    18541 accgccttta attaacgccc cacgacgcgc tgctgctgcc agcccggagg ttatcgccgc
    18601 aggcgttgag ataactaacg cacacgggca accaatcagc agcagcgtca gacctttata
    18661 aatccactcc tgccagctgg cggcaaacag cagcggcggc actagcgtta ccagcagggc
    18721 gacggccata atcgctggcg tataaatacg gctgaaacgg tcgataaacc gctcaatggg
    18781 ggcgcggcgc tcttcggctt cttcgatcag tttcagaatc cggtcaatgg cgctggcacc
    18841 cggttctgac agcacttcca gcgttaccag acggtctacg ctggtggctc ctgcaggaac
    18901 tttatcgccc gtcgcgcgct ccaccggaat ggattctccg gttagggcgc tttcatcaaa
    18961 actggcaaac ggtgagagca gtttaccgtc ggcaggcaaa cgcccacctg cggcgacttc
    19021 aatcacatcg ccaggccgca ggctgttaat cgccacctct tcccgctcac cgttacgcag
    19081 gcgcgtggcg gtttctggtt tcagcgccat taacgcgcta acgccctgac gcgcgcggct
    19141 ggcggcccag ccttccagtc gttcaccaat caaaaacagc agcaacacca tcgcagcttc
    19201 agccgttgcg ccaataaaca gtgcaccaat agcggctacg ctcattaagg tttcaatggc
    19261 gaagtagctg ccggatttga tcagccgtaa tgcctgacga gcaatcgggt acagcccaac
    19321 tagcgtggtc gcgataaacg ccagttgccc gaacggatga ttgaactgct ccagacccca
    19381 gctgattgcc atcatcacga taagcgtaat cagcggcaga ttctctttca ggcgcgatgc
    19441 ttgtggctcg tcggcggcct gctcatcgcg cagggaatag cccgcttttt gcaccgcaga
    19501 ttcaacctgg gcacggatgt cattgtcggc atcaaccacc agtttttcgg tggcgaacaa
    19561 cacctgcacc tgattcacgc ctgcaagctg gcgcacggca ttttctacct tgcgcgcaca
    19621 ggcggcgcag tccatgccgc tgactttcca gctatagcgg gtgccggaga cgttttcaga
    19681 gagcgttggc gagctggagc atgcgccgtc gcagcaacag tcgttggcgt tctgtaccgt
    19741 ggttagcggt ttgaacgcag caaattgagg ggctttcttg ccgtgattgt caggtgtcga
    19801 catggcatcc tccggttaag ttttttctca ttaaccgaag gatacactct ggagtcgact
    19861 ccagagtcaa gttttatcag agatacagtg agcggacgat gaggaagtga ccggcaaagt
    19921 agcaggccgc agcaatcgcg ttatccgcac ggaagcgtcg gcgatagtgg ctccccagcc
    19981 agacaaagtt actgataaac agcaacgaag cgccaacaaa cgcagagagc gccggagcgg
    20041 tcggacggaa gaaccacagt tcacctgcca gccacaccat caccagcgtc atgccgataa
    20101 acgtgcagat aggccaacgg tactcttcca gccgcgtcca gataatcgcc agtagcagcg
    20161 cacccagaac cagcaacacc agtggcagag gccagaagaa agagagcgtc atctgactgg
    20221 cgaaatagat ggtgtacagc aggtgcgaga ggaaaaacgc gccgatggcg tacatcagac
    20281 gttgacgtgg caacagggtt agcgcatcgc ccagcagtga ggcgcacagt cccgccagca
    20341 ccagatagct aatggcgtcg aacatcggtg cttgccaggc cagtaacagc aggagaagaa
    20401 gggttaacgg tttaaacacc cagcgttgcc aggttggccc acgatacgat gcatccacag
    20461 atagccatgc ggaaagacag acagcgataa acgaccaaag catcttagtt ccttgaattc
    20521 gttatttttt cgtaagcaga atgcctacgg tcttgcttcc agtttagtgg cgagggtgga
    20581 cggatgacaa caccataact ggcagggtat gcttaattcc gcattttccg atgagggata
    20641 aagatgagta aaccacctct tttctttatt gttatcattg gcttaattgt cgtcgccgca
    20701 tcgtttcgtt ttatgcaaca gcgacgggaa aaagctgata atgatatggc tccgctccag
    20761 caaaagctgg tggtggtgag caacaagcgg gaaaaaccga ttaacgatcg ccgttcgcgc
    20821 cagcaggaag tgactccggc aggcaccagt atgcgctatg aggcaagctt caaaccgcaa
    20881 agcggaggaa tggagcagac gtttcgcctc gacgcccagc agtaccacgc cctgacagtg
    20941 ggcgataaag gtacgctgag ctataaagga acgcgctttg ttagctttgt tggcgaacaa
    21001 taaggttatt ttttaacttt aaatttcttc tgccagacca gcagttcaaa cacgccgaaa
    21061 aggaaaatcc gcaccttttc ggcggtggtc atctgcgggc catctttcgg taaagtggat
    21121 ttcaatagcg ccagctgcat accatgcatc agcacggtaa aaatcagcgc cacgttaacg
    21181 aaaatattta gcgggcgcgg gaagggatgc accagattga ggattaaaaa tccccaaacg
    21241 cagagcatta acaaacgacc aatattaatc agcatcactt tctccttgtg cttcgtgttg
    21301 atacagccga taagccacct gacccgccac tttttcccga tgtaatgacc agtttgccgg
    21361 aacagtgggc agaccgtttt cgacttcgct ttcgacataa atcagggctt cgtcagccag
    21421 ccagccgtta tcttccagta aatttatcgt ctcttctaac aagccacggc ggaacggtgg
    21481 atcgacaaac acgatattat gcggtgtgcc tttttgcgcc aggaatgaca ttgcgttgct
    21541 gttcaccacg cgtgcattgc ctgcttttag tgtcgccaga ttcttaatta actgctgaga
    21601 aaccacgcga tccatctcaa tcaacgttgc ccccgcagcg tagcgcgata acgcttccag
    21661 ccccagcgcg ccgctcccgg cgaagcaatc cagacattgg gcgtcaacaa tgaccggagc
    21721 cagccagtta aacaacgttt cgcgtacgcg gtcggtggtg gggcgcagac ctgggctatc
    21781 aggaaccggg agtttacggc ctcgccactg cccgccaata atgcggattt ggccgctgcc
    21841 ggaatgattc ggttttttca tgataaattg ctcaatccgc cagataacac atacttgcag
    21901 gcggtgatgt gaattaagtt aagtgataga ctatttcatc atttttttag ctgctatgta
    21961 catagcgtta acgctgtgcc atgaagcaac agcgaggagt gtagtcgcaa atggcgaaag
    22021 aaaaaagacg tggctttttt tcctggctgg gctttggtca aaaagagcag accccggaaa
    22081 aagagacaga agttcagaat gaacaaccgg ttgtagaaga aatcgttcag gcgcaagagc
    22141 ctgcgaaggc ttctgaacaa gccgttgaag agcagccgca ggcgcatact gaagccgagg
    22201 cggaaacttt tgctgccgac gttgtggaag tcactgaaca ggttgttgaa agtgaaaaag
    22261 cgcagcctga agcggaagtt gttgcacagc cggaaccggt cgtagaagaa acgccggagc
    22321 cagtggctat cgaacgtgaa gagctgccgt tgccggaaga cgtcaacgcc gaagaggttt
    22381 cgtcagaaga gtggcaggct gaagcggaaa ccgtagagat tgtcgaagcg gcagaagaag
    22441 aagcggctaa agaagaaatc accgacgaag agctggaagc acaggcgctg gctgccgaag
    22501 cggcagaaga ggcggtgatg gtagttcctc cggcagaaga agagcagccg gttgcagaaa
    22561 ttgcccagga gcaagaaaaa ccgaccaaag aaggcttctt cgcgcgcctg aaacgcagcc
    22621 tgttaaaaac caaagaaaat ctcggttccg gatttatcag cctgttccgc ggtaaaaaaa
    22681 tcgacgatga tctgtttgaa gagctggaag aacagctgtt gatcgccgat gtgggcgtgg
    22741 aaaccacacg taaaattatc accaatctga cggaaggcgc atcccgcaag cagcttcgtg
    22801 atgccgaggc gctctatggc ctgctgaaag aagagatggg cgagattctg gcgaaagtcg
    22861 atgagccgct gaatgttgaa gataaaacgc cgttcgtgat cctgatggtg ggcgtcaacg
    22921 gtgtgggtaa aaccacgacg attggtaagc tggcgcgtca gtttgagcag cagggtaaat
    22981 cggtgatgct ggcggcgggc gatactttcc gtgcagctgc ggttgaacag cttcaggtct
    23041 ggggtcagcg caacaatatt ccggtgattg cccagcatac cggtgcggat tccgcctctg
    23101 ttatcttcga cgccattcag gcagctaaag cgcgtaatat cgacgtcctg attgccgata
    23161 ccgccggacg tctgcagaac aaatcgcatc tgatggaaga gttgaagaaa atcgtccgcg
    23221 tgatgaagaa actcgacgtt gaagcgccgc atgaagttat gctgactatt gatgccagca
    23281 ccgggcagaa cgcggtaagc caggccaaac tgttccacga agctgttggc ttaaccggca
    23341 tcacgctaac gaaactggac ggcacggcga aaggcggggt aattttctcg gtggctgacc
    23401 agtttggtat ccctatccgc tacattggtg tcggcgaacg tattgaggat ttgcgtccgt
    23461 ttaaggcgga cgactttata gaggcacttt ttgcccgaga ggattaacaa tgattcgctt
    23521 tgaacatgtc agcaaggctt atctcggtgg gagacaggcg ctgcagggcg ttacgttcca
    23581 tatgcagccg ggtgagatgg cgtttctgac cggtcattcc ggcgcaggga aaagtaccct
    23641 cctgaagctg atctgtggga ttgagcggcc cagcgccggg aaaatctggt ttagcggcca
    23701 tgacatcacg cgtctgaaaa accgtgaagt tccgtttctg cgccgccaga ttggcatgat
    23761 tttccaggat caccatctac tgatggaccg tactgtctac gataacgtgg cgatcccgct
    23821 gattatcgct ggtgccagcg gtgacgatat tcgtcgccgg gtgtcggcgg cactggataa
    23881 agtcgggcta ctggacaaag cgaagaactt ccctattcag ctttccggcg gtgaacaaca
    23941 gcgtgttggc attgcccgcg cggtggtgaa taagcccgcg gtactgctgg cggacgaacc
    24001 gaccggtaac ctggacgacg cgttgtcgga agggattcta cgtctgtttg aagagtttaa
    24061 ccgcgttggg gtaaccgtat tgatggcaac gcacgacatt aatctgattt cacggcgttc
    24121 ctatcgcatg ctcaccctga gcgatggtca cttgcatgga ggcgtgggcc atgaataagc
    24181 gcgatgcaat caatcatatt cggcagtttg gtgggcgtct ggatcgcttc cgtaaatcgg
    24241 tcggcggctc aggcgacggc ggtcgtaacg caccgaaacg cgcgaaatcc tcgccaaaac
    24301 cggtaaatcg caaaaccaac gttttcaacg aacaggtgcg ctatgccttc cacggcgcat
    24361 tgcaggatct gaaaagcaaa ccgttcgcca cgtttttaac ggtgatggtt atcgccatct
    24421 ccctgacgct gcccagcgtc tgttatatgg tgtacaaaaa cgttaatcag gcggcgacgc
    24481 agtattatcc gtcaccgcaa atcactgttt acttgcaaaa aacgctggac gatgacgccg
    24541 ctgcgggcgt ggtggcacag ttgcaggccg agcaaggcgt ggagaaagtg aactatcttt
    24601 ctcgtgaaga cgcactgggc gagttccgta actggtctgg ttttggtggt gcgctggata
    24661 tgctggaaga aaacccgctt ccggcagtgg cggtggtgat cccgaaactc gattttcagg
    24721 ggacggaatc actcaatacg ctgcgtgatc gtatcacgca gattaacggt attgacgaag
    24781 tgcggatgga tgacagctgg tttgcccgtc tggcggcgtt gaccgggctg gttgggcgcg
    24841 tttcggcgat gatcggcgtg ttgatggtgg cggcagtgtt cctcgtcatc ggcaacagcg
    24901 tacgtctgag catctttgct cgccgtgact ccatcaacgt gcagaaactg attggtgcga
    24961 cggatggttt catccttcgc ccgttcctgt acggcggtgc gctgctggga ttttctggcg
    25021 cattgttgtc attaattttg tcagaaattc tggtactgcg tttgtcgtca gcggtggcgg
    25081 aagtggcaca ggttttcgga acgaagtttg atatcaatgg tttatcgttc gatgaatgcc
    25141 tgctattgct gctggtatgc tcgatgattg gctgggtggc agcgtggctt gccacggtac
    25201 aacatttacg ccactttacg cctgaataat aaaagcgtgt tatactcttt ccctgcaatg
    25261 ggttccgtag cagggaaaga gaccccgttg tctcttcccg gtatttcatc tctatgtcac
    25321 attttgtgcg taatttattc acaagcttgc attgaacttg tggataaaat cacggtctga
    25381 taaaacagtg aatgataacc tcgttgctct taagctttgg cacagttgtt gctaccactg
    25441 aagcgccaga agatatcgat tgagaggatt tgaatgactg acaaaatgca aagtttagct
    25501 ttagccccag ttggcaacct ggattcctac atccgggcag ctaacgcgtg gccgatgttg
    25561 tcggctgacg aggagcgggc gctggctgaa aagctgcatt accatggcga tctggaagca
    25621 gctaaaacgc tgatcctgtc tcacctgcgg tttgttgttc atattgctcg taattatgcg
    25681 ggctatggcc tgccacaggc ggatttgatt caggaaggta acatcggcct gatgaaagca
    25741 gtgcgccgtt ttaacccgga agtgggtgtg cgcctggtct ccttcgccgt ccactggatc
    25801 aaagcagaga tccacgaata cgttctgcgt aactggcgta tcgtcaaagt tgcgaccacc
    25861 aaagcgcagc gcaaactgtt cttcaacctg cgtaaaacca agcagcgtct gggctggttt
    25921 aaccaggatg aagtcgaaat ggtggcccgt gaactgggcg taaccagcaa agacgtacgt
    25981 gagatggaat cacgtatggc ggcacaggac atgacctttg acctgtcttc cgacgacgat
    26041 tccgacagcc agccgatggc accggtgctc tatctgcagg ataaatcatc taactttgcc
    26101 gacggcatcg aagatgataa ctgggaagag caggcggcaa accgtctgac cgacgcgatg
    26161 catggtctgg acgaacgcag ccaggacatc atccgtgcgc gctggctgga cgaagacaac
    26221 aagtccacgt tgcaggaact ggctgaccgt tacggtgttt ccgctgaacg tgtgcgccaa
    26281 ctggaaaaga acgcgatgaa aaaacttcgc gccgctattg aagcgtaatt tccgctatta
    26341 agcagagaac cctggatgag agtccggggt ttttgttttt tgggcctcta caataatcaa
    26401 ttccccctcc ggcaaaacgc caatccccac gcagattgtt aataaactgt caaaatagct
    26461 attccaatat cataaaaatc gggtatgttt tagcagagta tgctgctaaa gcacgggtag
    26521 tcatgcataa aacgaaataa agtgctgaac aacaacatca caacacacgt aataaccaga
    26581 agaatgggga ttctcaggat gaacataaag ggtaaagcgt tactggcagg atgtatcgcg
    26641 ctggcattca gcaatatggc tctggcagaa gatattaaag tcgcggtcgt gggtgcaatg
    26701 tccggtccgg ttgcgcagta cggtgaccag gagtttaccg gcgcagagca ggcggttgcg
    26761 gatatcaacg ctaaaggcgg cattaaaggc aacaaactgc aaatcgtaaa atatgacgat
    26821 gcctgtgacc cgaaacaggc ggttgcggtg gcgaacaaag tcgttaacga cggcattaaa
    26881 tatgtgattg gtcacctctg ttcttcatca acgcagcctg cgtctgacat ctacgaagac
    26941 gaaggcattt taatgatcac cccagcggca accgcgccgg agctgaccgc ccgtggctat
    27001 cagctgatcc tgcgcaccac cggcctggac tccgaccagg ggccgacggc ggcgaaatat
    27061 attcttgaga aagtgaaacc gcagcgtatt gctatcgttc acgacaaaca gcaatacggc
    27121 gaaggtctgg cacgagcggt gcaggacggc ctgaagaaag gcaatgctaa cgtggtgttc
    27181 tttgatggta tcaccgccgg ggaaaaagat ttctcaacgc tggtggcgcg tctgaaaaaa
    27241 gagaatatcg acttcgttta ctacggcggt tatcacccgg aaatggggca aatcctgcgt
    27301 caggcacgcg cggcagggct gaaaactcag tttatggggc cggaaggtgt ggctaacgtt
    27361 tcgctgtcta acattgcggg cgaatcagcg gaagggctgc tggtgaccaa gccgaagaac
    27421 tacgatcagg ttccggcgaa caaacccatt gttgatgcga tcaaagcgaa aaaacaggac
    27481 ccaagcggcg cattcgtctg gaccacctac gccgcgctgc aatctttgca ggcgggcctg
    27541 aatcagtctg acgatccggc tgaaatcgcc aaatacctaa aagcgaactc cgtggatacc
    27601 gtgatggggc cactgacctg ggatgagaaa ggcgatctaa agggctttga gttcggcgta
    27661 tttgactggc acgccaacgg cacggcgacc gatgcgaagt aattgttaat cggcaacttt
    27721 gggttgccgc caaattgcta atatcgaata cgttgcttca tgccggatgc ggcgtaaacg
    27781 ccttatccgg cctacaagaa ccaaagaaat cagtaaattg cagcacacat tataggcctg
    27841 ataagcgtag cgcatcaggc aatttctcgt tggtcatcac ttttgaaatc aacttgacga
    27901 ttaacgcttc tcccagccgc cctgctgtgc cgtaaatccc agtgcctgca taaacgccgt
    27961 catcacaccg cgatcttcca cgcctgcatc cgccatccac cagcatgaaa cgccaggatt
    28021 gttacgcaaa acctcttcca gcagatattg ccccacaccg cgacggcggg tgacttcccg
    28081 cacgcgtaga gaatccagtg ctccctcggt gccgcttaag gttacccgca cggccgcaag
    28141 cagacgctcg ttaaaacgcg cggcgtagat acggtggtta tcgtcaacct gtaaagagga
    28201 aggggaatac tccggccaga tcttttgcag gtcaatcc
//
LOCUS       DAZPAB010000033        28197 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZPAB010000033 DAZPAB010000000
VERSION     DAZPAB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28197
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.033"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..209)
                     /locus_tag="ACHUPG_001284"
     CDS             complement(<1..209)
                     /locus_tag="ACHUPG_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR0860968.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHUPG_001285"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHUPG_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR0860969.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHUPG_001286"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHUPG_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR0860970.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHUPG_001287"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHUPG_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR0860971.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHUPG_001288"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHUPG_001288"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR0860972.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHUPG_001289"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHUPG_001289"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR0860973.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHUPG_001290"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHUPG_001290"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR0860974.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="ACHUPG_001291"
     CDS             complement(4598..5320)
                     /locus_tag="ACHUPG_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0860975.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHUPG_001292"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHUPG_001292"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0860976.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="ACHUPG_001293"
     CDS             6520..6738
                     /locus_tag="ACHUPG_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR0860977.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHUPG_001294"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHUPG_001294"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR0860978.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="ACHUPG_001295"
     CDS             complement(7472..7672)
                     /locus_tag="ACHUPG_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR0860979.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACHUPG_001296"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="ACHUPG_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACHUPG_001297"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="ACHUPG_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR0860980.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="ACHUPG_001298"
     CDS             10101..12014
                     /locus_tag="ACHUPG_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0860981.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="ACHUPG_001299"
     CDS             12014..13036
                     /locus_tag="ACHUPG_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR0860982.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="ACHUPG_001300"
     CDS             13030..13248
                     /locus_tag="ACHUPG_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR0860983.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="ACHUPG_001301"
     CDS             13302..14171
                     /locus_tag="ACHUPG_001301"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR0860984.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="ACHUPG_001302"
     CDS             complement(14226..14630)
                     /locus_tag="ACHUPG_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR0860985.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="ACHUPG_001303"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="ACHUPG_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR0860986.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="ACHUPG_001304"
     CDS             15615..17705
                     /locus_tag="ACHUPG_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR0860987.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD"
                     /locus_tag="ACHUPG_001305"
                     /gene_synonym="dapC"
     CDS             complement(17772..18992)
                     /gene="argD"
                     /locus_tag="ACHUPG_001305"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR0860988.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="ACHUPG_001306"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="ACHUPG_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR0860989.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="ACHUPG_001307"
     CDS             complement(19673..20275)
                     /locus_tag="ACHUPG_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR0860990.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="ACHUPG_001308"
     CDS             complement(20265..20432)
                     /locus_tag="ACHUPG_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR0860991.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="ACHUPG_001309"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="ACHUPG_001309"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR0860992.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22569
                     /gene="tsgA"
                     /locus_tag="ACHUPG_001310"
                     /pseudo
     CDS             21389..22569
                     /gene="tsgA"
                     /locus_tag="ACHUPG_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
     gene            22831..25374
                     /gene="nirB"
                     /locus_tag="ACHUPG_001311"
     CDS             22831..25374
                     /gene="nirB"
                     /locus_tag="ACHUPG_001311"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR0860993.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25371..25697
                     /gene="nirD"
                     /locus_tag="ACHUPG_001312"
     CDS             25371..25697
                     /gene="nirD"
                     /locus_tag="ACHUPG_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR0860994.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25823..26629
                     /gene="nirC"
                     /locus_tag="ACHUPG_001313"
     CDS             25823..26629
                     /gene="nirC"
                     /locus_tag="ACHUPG_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR0860995.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26648..28021
                     /gene="cysG"
                     /locus_tag="ACHUPG_001314"
     CDS             26648..28021
                     /gene="cysG"
                     /locus_tag="ACHUPG_001314"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR0860996.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6845 a         7538 c         7101 g         6713 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaaga gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgctaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa gaaaagtggg ggatcggcgt actgtttctc
    22021 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22081 tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg
    22141 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22201 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22261 acaccagtac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22321 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22381 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcacgatt
    22441 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22501 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22561 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22621 cgctacgtgc atctatttct ataaacccgt tcattttgtc cattttttgc acaaacatga
    22681 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22741 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22801 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22861 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22921 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctca
    22981 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23041 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23101 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcaaccggt
    23161 tcctacccgt ggatcccacc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23221 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23281 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23341 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23401 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23461 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23521 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23581 acccagtgtg gtctggacgt tgctccgcgt ggggggattg tcattaatga ttcctgccag
    23641 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23701 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctgggtagc
    23761 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23821 ggtggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctatgt ttacctcgac
    23881 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    23941 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaacgct
    24001 atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg tagcggtaag
    24061 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24121 actaaaggtg atctgattgc tgccatcaac aaaggctgcc acacagttgc ggcgctgaaa
    24181 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24241 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24301 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24361 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg
    24421 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24481 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgt
    24541 tctccgggcg gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat cgcgcgtgaa
    24601 tttaatctct acaccaaaat caccggctcc cagcgcctgg cgatgtttgg cgcacagaaa
    24661 gacgatctgc cggaaatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24721 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggt
    24781 gttggcgata gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24841 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24901 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    24961 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25021 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25081 gcgccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25141 gacaaactgg ggctgaacgc tcatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25201 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25261 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25321 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25381 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcactgttag
    25441 gtgacaagca ggtagcgatt ttccgcccgt atcacagcga ccaggtgttt gcgatcagca
    25501 acattgatcc gttcttcgag tccagcgtgc tatcacgcgg gctgattgcg gaacaccagg
    25561 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25621 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25681 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25741 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25801 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaatgc
    25861 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25921 ggcgggcgca tatgtgggtc ttgggatcat cctgattttc acgctcggta atttgctcga
    25981 cccgtccgta cgtccgctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26041 tatcgccggt tctgaactgt tcaccggaca caccatgttc ctcacctttg gggtaaaagc
    26101 gggcagcatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26161 ggtcggttcc gtcttcgttg ccatgctcta tagctggggc ggcggtagcc tgctgccggt
    26221 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26281 gttcttcaaa ggtgcattgt gtaactggct ggtttgcctg gcaatctgga tggcgctgcg
    26341 cactgaaggg gcggcgaaat ttatcgctat ctggtggtgt ctgctggcat ttatcgcgtc
    26401 cggctacgag cactctatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26461 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26521 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26581 tcgtccggtt gcggacaaat ttaatcaaac tgaaacagct gccggttaat tactaagggg
    26641 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26701 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26761 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26821 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26881 agcgacggat gatgacacgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    26941 cttctgtaac gtagttgatg cgccgaaagc cgccagcttt attatgccgt cgattattga
    27001 ccgctcaccg ctaatggtag cggtctcctc tggcggcacc tctccggttc tggcgcgcct
    27061 gttgcgcgaa aaactcgaat cactgctgcc gttgcatctg ggccaggtgg cgaaatatgc
    27121 cgggcaatta cgcgggcgag tgaaacaaca gttcgccacg atgagtgagc gtcgccgttt
    27181 ctgggagaaa ttgttcgtta acgaccgcct ggcgcagtcg ctggcaaaca acgatcagaa
    27241 agccattact gaaacgaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27301 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acgctgaaag gactgcaaca
    27361 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27421 ggtacgccgc gatgctgatc gcgttttcgt cggcaaacgc gcgggatacc actgtgtacc
    27481 gcaggaagag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg
    27541 gctgaaaggt ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27601 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctctgc
    27661 ctattcgggt attccactca cgcatcgcga ttatgcccag agcgtacgct taattaccgg
    27721 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27781 ggtgttctat atggggttga atcaggccgc gactattcag caaaagctga ttgaacacgg
    27841 tatgcctggc gaaatgccgg tggcaattgt cgaaaacgga acggcagtca cgcagcgcgt
    27901 gattgacggt acgctcacgc aactcggtga acttgctcag caaatgaaca gtccatcgct
    27961 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaatcatta
    28021 atttaacccg ggccagagaa ttctggcctt cttaatgact ctttttatat attcagcaaa
    28081 taaaacataa ccccttaata aatattatct gctaatgggt tatattcacc gttacagcga
    28141 tttacaaaat taagtcattc gctcactgac gccaattaaa tattcctgta tcgttct
//
LOCUS       DAZPAB010000034        27601 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZPAB010000034 DAZPAB010000000
VERSION     DAZPAB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.034"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="ACHUPG_001315"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="ACHUPG_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR0860997.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="ACHUPG_001316"
     CDS             complement(1614..1937)
                     /locus_tag="ACHUPG_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR0860998.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACHUPG_001317"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="ACHUPG_001317"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR0860999.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACHUPG_001318"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="ACHUPG_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR0861000.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACHUPG_001319"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="ACHUPG_001319"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR0861001.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACHUPG_001320"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="ACHUPG_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR0861002.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="ACHUPG_001321"
     CDS             7537..8574
                     /locus_tag="ACHUPG_001321"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR0861003.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVLDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACHUPG_001322"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="ACHUPG_001322"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR0861004.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="ACHUPG_001323"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="ACHUPG_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR0861005.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACHUPG_001324"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="ACHUPG_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR0861006.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="ACHUPG_001325"
     CDS             10745..11626
                     /locus_tag="ACHUPG_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0861007.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACHUPG_001326"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="ACHUPG_001326"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR0861008.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="ACHUPG_001327"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="ACHUPG_001327"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR0861009.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACHUPG_001328"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="ACHUPG_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="ACHUPG_001329"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="ACHUPG_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR0861010.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="ACHUPG_001330"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="ACHUPG_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR0861011.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="ACHUPG_001331"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="ACHUPG_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR0861012.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="ACHUPG_001332"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="ACHUPG_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR0861013.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="ACHUPG_001333"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="ACHUPG_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR0861014.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="ACHUPG_001334"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="ACHUPG_001334"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR0861015.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="ACHUPG_001335"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="ACHUPG_001335"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR0861016.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="ACHUPG_001336"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="ACHUPG_001336"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR0861017.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="ACHUPG_001337"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="ACHUPG_001337"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR0861018.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="ACHUPG_001338"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="ACHUPG_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR0861019.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACHUPG_001339"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="ACHUPG_001339"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR0861020.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /gene="acpS"
                     /locus_tag="ACHUPG_001340"
     CDS             24893..25273
                     /gene="acpS"
                     /locus_tag="ACHUPG_001340"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR0861021.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="ACHUPG_001341"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="ACHUPG_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR0861022.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="ACHUPG_001342"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="ACHUPG_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFR0861023.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27538
                     /locus_tag="ACHUPG_001343"
                     /pseudo
     CDS             26442..27538
                     /locus_tag="ACHUPG_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6806 a         6866 c         7237 g         6692 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 ctggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcggcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagccactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaaaatg
    27541 aggtgtactg gcaatagcgg acactaccat ttgttctttt tttaagcagc catctgatga
    27601 t
//
LOCUS       DAZPAB010000035        26563 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZPAB010000035 DAZPAB010000000
VERSION     DAZPAB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.035"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACHUPG_001344"
     CDS             complement(<1..106)
                     /gene="pliG"
                     /locus_tag="ACHUPG_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR0861024.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGH"
     gene            348..641
                     /locus_tag="ACHUPG_001345"
     CDS             348..641
                     /locus_tag="ACHUPG_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR0861025.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="ACHUPG_001346"
     CDS             713..1372
                     /locus_tag="ACHUPG_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR0861026.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="ACHUPG_001347"
     CDS             1464..1910
                     /locus_tag="ACHUPG_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR0861027.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="ACHUPG_001348"
                     /pseudo
     CDS             complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="ACHUPG_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3390..3809
                     /locus_tag="ACHUPG_001349"
     CDS             3390..3809
                     /locus_tag="ACHUPG_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR0861028.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3809..5077
                     /gene="umuC"
                     /locus_tag="ACHUPG_001350"
     CDS             3809..5077
                     /gene="umuC"
                     /locus_tag="ACHUPG_001350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR0861029.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="ACHUPG_001351"
     CDS             complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="ACHUPG_001351"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR0861030.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="ACHUPG_001352"
     CDS             complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="ACHUPG_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR0861031.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7561..8280
                     /gene="fadR"
                     /locus_tag="ACHUPG_001353"
     CDS             7561..8280
                     /gene="fadR"
                     /locus_tag="ACHUPG_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR0861032.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8332..9864)
                     /locus_tag="ACHUPG_001354"
     CDS             complement(8332..9864)
                     /locus_tag="ACHUPG_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR0861033.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10194..11492
                     /gene="dadA"
                     /locus_tag="ACHUPG_001355"
     CDS             10194..11492
                     /gene="dadA"
                     /locus_tag="ACHUPG_001355"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR0861034.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11502..12572
                     /gene="dadX"
                     /locus_tag="ACHUPG_001356"
     CDS             11502..12572
                     /gene="dadX"
                     /locus_tag="ACHUPG_001356"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR0861035.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12740..14154)
                     /locus_tag="ACHUPG_001357"
                     /pseudo
     CDS             complement(12740..14154)
                     /locus_tag="ACHUPG_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14765..16501)
                     /locus_tag="ACHUPG_001358"
     CDS             complement(14765..16501)
                     /locus_tag="ACHUPG_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR0861036.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="ACHUPG_001359"
     CDS             complement(16596..17510)
                     /gene="ldcA"
                     /locus_tag="ACHUPG_001359"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR0861037.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17610..18221
                     /gene="emtA"
                     /locus_tag="ACHUPG_001360"
     CDS             17610..18221
                     /gene="emtA"
                     /locus_tag="ACHUPG_001360"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR0861038.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18223..18957)
                     /locus_tag="ACHUPG_001361"
     CDS             complement(18223..18957)
                     /locus_tag="ACHUPG_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR0861039.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19158..19412
                     /locus_tag="ACHUPG_001362"
     CDS             19158..19412
                     /locus_tag="ACHUPG_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR0861040.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19462..21416)
                     /locus_tag="ACHUPG_001363"
                     /pseudo
     CDS             complement(19462..21416)
                     /locus_tag="ACHUPG_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            complement(21442..22296)
                     /gene="modD"
                     /locus_tag="ACHUPG_001364"
     CDS             complement(21442..22296)
                     /gene="modD"
                     /locus_tag="ACHUPG_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFR0861041.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22296..23105)
                     /locus_tag="ACHUPG_001365"
     CDS             complement(22296..23105)
                     /locus_tag="ACHUPG_001365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0861042.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23115..23873)
                     /locus_tag="ACHUPG_001366"
     CDS             complement(23115..23873)
                     /locus_tag="ACHUPG_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0861043.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23870..24850)
                     /locus_tag="ACHUPG_001367"
     CDS             complement(23870..24850)
                     /locus_tag="ACHUPG_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFR0861044.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24850..25872)
                     /locus_tag="ACHUPG_001368"
     CDS             complement(24850..25872)
                     /locus_tag="ACHUPG_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFR0861045.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26222..>26563
                     /locus_tag="ACHUPG_001369"
     CDS             26222..>26563
                     /locus_tag="ACHUPG_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861046.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
BASE COUNT         6674 a         6471 c         6855 g         6563 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgcga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcttgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc tgcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgttttatct
     3001 gcaacgattt cagtcataat cattcgcctc tttaaatata taaattgtaa tgaaactcct
     3061 gttttacaac tattaataaa ttttacttca tctaattcat agttagccgg gcgggatgcg
     3121 tcaatgtctt tatttctatt aatatgataa atatcaaaca atgtttaatg tcattatggc
     3181 gaatgcttct attctatttt tttgccgggt gatatttttc atttctgctg gatgagcgtc
     3241 gtcgccagaa ggccacgtga gcacaggata agagaacgaa aaatcagcag tctatgcagc
     3301 gacaaatatt gatagcctga atcagtattg atctgctggc aagaacagac cactgtatat
     3361 aaaaacagta taacttcagg cagattatta tgttgtttat caagcctgcg gatctccgcg
     3421 aaattgtgac ttttccgcta tttagcgatc ttgttcagtg tggctttcct tcaccggcag
     3481 cagattacgt tgaacagcgc atcgatctga atcaactgtt gatccagcat cccagcgcga
     3541 cttacttcgt caaagcaagt ggtgattcta tgattgatgg tggaattagt gacggtgatt
     3601 tactgattgt cgatagtgct attaccgcca gccatggtga tattgtcatc gctgctgttg
     3661 acggcgagtt tacggtgaaa aaattgcaac tacgcccgac ggtacagctt attcccatga
     3721 acagtgcgta ctcgcccatt accatcagta gcgaagatac gctggatgtc tttggtgtgg
     3781 tgatccacgt cgttaaggcg atgcgctgat gtttgccctc tgtgatgtaa acgcgtttta
     3841 tgccagctgt gagacggtgt ttcgccctga tttatggggt aaaccggtgg ttgtgctatc
     3901 gaataatgac ggttgcgtta tcgcccgaaa cgctgaggca aaggcgctgg gcgttaaaat
     3961 gggcgatccc tggttcaaac aaaaagatct gtttcgtcgc tgtggtgtgg tttgctttag
     4021 cagcaattat gagctttacg cagacatgag caatcgggtg atgtcgacgc tggaagaact
     4081 atcgccccgc gtcgagattt acagtattga tgaagcattc tgcgatctga caggtgtgcg
     4141 taattgtcgc gatctgactg attttggcag agaaattcgc gcaacggtgc tacaacgtac
     4201 ccatcttact gttggtgtgg ggatcgccca gaccaaaacg ttggctaagc ttgccaatca
     4261 tgcggcaaaa aaatggcagc ggcagacggg tggggtggtg gatttatcaa atctggaacg
     4321 ccagcgtaaa ttaatgtctg ctctccccgt ggatgacgtc tgggggattg gacggcggat
     4381 cagcaaaaaa ctggacgcga tggggatcaa aaccgttctc gatttggcgg atacagatat
     4441 ccggtttatc cgtaaacatt ttaatgtcat gctcgaaaga acggtgcgtg aactgcgcgg
     4501 cgaaccctgt ttgcaactgg aagagtttgc accgacgaag caggaaatta tctgttcccg
     4561 ctcgtttggt gaacgcatca cggattatac gtcgatgcgg caggccattt gtagttacgc
     4621 tgcccgggcg gcggaaaaac ttcgcagtga gcatcaatat tgtcggttta tctccacatt
     4681 tattaagacg tcaccatttg cgctcaatga accttattac ggcaatagcg cgtcggtaaa
     4741 actgctgacg cccactcagg acagcaggga tatcattaac gctgctacgc gatctctgga
     4801 tgccatctgg caagcgggcc atcgctacca aaaagcgggc gtgatgctgg gggatttctt
     4861 cagtcaggga gtcgcgcagc tcaatttatt cgatgacaac gcaccgcgcc ccgggagtga
     4921 gcaattgatg gcggtaatgg atactctgaa tgctaaagag ggcagaggaa cactctattt
     4981 tgccgggcag gggatccaac aacaatggca gatgaagcga gctatgcttt caccacgtta
     5041 tacaacgcga agttctgatt tactgcgggt caaataaata tagcggcagg aaaaaagcgc
     5101 tcccgcagga gcgccgaatg gattagcgac cgaacagatc acgttttttc gctttaaacg
     5161 gctgggaaat caccaccagc accgcgacaa tcaggtaagc gataaaaata ccgagcagcc
     5221 actgcggcat ttccatacct aaaaaatccc actgacgctc ggcgcaatcg ccagaggcga
     5281 caaacacttg cggcacccat ttatccagcg gcagccattc cgggaaacga accataaaat
     5341 cacaggtggc aaatggcgaa ggatagagct gaagcatggt gtgctcgtaa gttaactgca
     5401 caccgcggaa cgcactatac aaccagataa ccatcgctac ataacgcagc ggagttttcg
     5461 gggcgatcgc gccaatcagc gcagcaccca gaacgccgaa taacgcgcag cgttcataaa
     5521 tacagagcac gcaaggtttc agtaacatca catgctggaa ccacagcgcc gtcagttcca
     5581 gtgccagagc agtaaacgcc atcaacagcc acgcgccccg gccttgtgaa cattggttca
     5641 aaaatcgcaa cataatcatt tccctgcaat atgcatagag tgcgcagttt aaaccaattc
     5701 attcgctgcg ccaccaggag gcagcaaaaa taggacgcaa ttggacaatt atcgggcgaa
     5761 aaggcaaacc gggcatttat atgcccggta agttgttatc aaagcgttgc tatccagccc
     5821 atttgcataa accattcggt tacaggggca agcgtaaact cgacgcagag caagccgacg
     5881 agtgtcagta cgagggtgta aggcagggcc atccacacca tgcggccata agagaggcga
     5941 atcaatggcg cgagtgcaga ggtcagcagg aacaggaacg cagcctgacc gttcggcgta
     6001 gcgacagagg gcagattggt accggtatta atggcgaccg ccagcagctc gtattgcttc
     6061 aacgtgatag cgccactttc cattgccgct ttcgcttcgt tgatataaat cgtccccacg
     6121 aagacgttat ccgaaatgga tgacagcaga ccgttgaaaa tatagaacag cgacagctga
     6181 gcatgttcag atgcctgcaa cacaaactga ataatcggcg aaaacagttg ttggtcgata
     6241 atcaccgcga ctaccgagaa aaacaccgtc aacagtgcgg tgaatggcag agattcggtg
     6301 aaggctttac cgatagcatg ttcatcggtg acaccggtca atgatgttgc cagaataatg
     6361 actgacaaac caatcaagcc aacttccgcc agatgcaacg ccagcgcagt caccagccag
     6421 acgccaataa tcgcctggac aatcagacgg attttatcct gacgggtacg ctggtggcgg
     6481 ctttgatcgt caaactgttg caacacttcg cggactttct ccggcagcgt ttcaccgtag
     6541 ccaaaccaac gcagcttctc taccagcagg caggttaaca ggccacaaat cagaaccgga
     6601 acggtcaccg gcgacatgcg caggaagaaa tcgccaaaat gccagccagc cgctttagcg
     6661 atgatcaggt tctgtggttc gcccaccatg gtcattacgc cgcctaatgc ggtgccgaca
     6721 ccggcatgca tcatcaggct acgcagaaag ccacggaact gttccagaac cacgttgtaa
     6781 tgcttgtcga tatgactatc gtcttgcagg tcggtgtctt cggtacggga agaggctacg
     6841 cgatgataaa taccataaaa accgactgca acgctgatca ccaccgccac gacggttaag
     6901 gcatcgagga acgcggagag gaacgctgcc gccacgcaaa aagagagcga cagcagcatt
     6961 ttggagcgaa tgcttaacag caaacgggta aatatgaaca gcaacagctg tttcataaaa
     7021 tagatacccg ccaccataaa catcaataac agcaagactt caagatttgc cgccacctct
     7081 tcacggacgt gttccgcgct ggtcatgccg atgaataccg cttcgatagc caacagacca
     7141 ccggggagta gcgggtagca tttcagggcc atcgccagag tgaaaataaa ttccgctacc
     7201 agcaaccagc ccgcgacgaa agggctgatg aggaaaatta acgggtttac gattaagaaa
     7261 atgatgaggg cgagtttgta ccagtcgggg gactggccca aaaaattgcg ccatagcgcg
     7321 cggccccagg agatctccat gatggtttcc cttaccttac aaataatcaa tgatgttttt
     7381 atgtttaaac gcaaagctta acggtcaggc aggagtgagg caagtcttga tagtcaaggg
     7441 gaaagagatg cggaaaatga agccttgatc cctttttctt ctttttgtct gctatcagcg
     7501 tagttagccc tctggtatga tgagtccaac tttgttttgc tgtgttatgg aaatctcact
     7561 atggtcatta aggcgcaaag cccggcgggt ttcgcggaag agtacattat tgaaagtatc
     7621 tggaataacc gcttccctcc cgggactatt ttgcccgcag aacgtgaact ttcagaatta
     7681 attggcgtaa cgcgtactac gttacgtgaa gtgttacagc gtctggcacg agatggctgg
     7741 ttgaccattc aacatggcaa gccgacgaag gtgaataatt tctgggaaac ttccggttta
     7801 aatatccttg aaacactggc gcgactggat cacgaaagtg tgccgcagct tattgataat
     7861 ttgctgtcgg tgcgtaccaa tatttccact atttttattc gcaccgcgtt tcgtcagcat
     7921 cccgataaag cgcaggaagt gctggctacc gctaatgaag tggccgatca cgccgatgcc
     7981 tttgccgagc tggattacaa catattccgc ggcctggcgt ttgcttccgg caacccgatt
     8041 tacggtctga tccttaacgg gatgaaaggg ctgtatacgc gcattggtcg tcactatttc
     8101 gccaatccgg aagcgcgcag tctggcgctg ggcttctacc acaaactgtc ggcgttgtgc
     8161 agtgaaggcg cgcacgatca ggtgtacgaa acagtgcgtc gctatgggca tgagagtggc
     8221 gagatttggc accggatgca gaaaaatctg ccgggtgatt tagccattca ggggcgataa
     8281 tcccttccgt ttaaagagca accccctcaa acgaggggtt ttttgttgtt tttacagatt
     8341 tcccattctt ggcgggcaac gttccagcaa ctcgatgctg ccgtcttcgt tttgctgttc
     8401 gagcatcaca tcaaatcccc acaggcgatg cacatgcttc aggacttctt tgcgcccccg
     8461 atccagcggt gcgcgattat gtggaatata acgcagcgtc agcgaacggt cgccgcgcaa
     8521 atccacgttc cagatctgaa tattcggctc cagattactt aagttatatt gcgacgataa
     8581 ccggttacgg atctcccgat aaccttcttc attatgaata gcggaaatct ccagataatt
     8641 atgccgatca tcatccagca cggtgaagaa gcggaaatca cgcatcactt tcggtgacag
     8701 aaactggctg ataaaactct catctttgaa atcacgcatc gcgaaatgca gcgtttccag
     8761 ccagtcggaa ccggcgatat ccgggaacca gtatttgtct tcttccgttg gcgactgaca
     8821 aatccgttta atatcctgga acatggcgaa cccgagggca tacgggttga tgccgctgta
     8881 ccacgggctg ttataggggg gctggaagac cacattggtg tggctgtgca aaaactccag
     8941 cataaaacgt tccgttactt tcccttcatc atacagatgg ttaaggatgg tatagtgcca
     9001 gaaggtcgca cagccttcgt tcatcacctg agtctgtttt tgcggataaa aatactggct
     9061 caccttacgc acaatacgca ggatttcacg ctgccatgat tccagcagtg gcgcattttt
     9121 ctccataaaa tagagcaggt tttcttgtgg ttcggacgga tagcggcgcg cttcagcaac
     9181 cgttttctct tcctcgcgct tcggcagggt acgccagagc atattgacct gactttgtag
     9241 atactcttcg cgacttttct gccgggcttt ctcttcttgc agcgagattt tttgcgggcg
     9301 tttgtaccgg tccacgccgt agttcatcag cgcgtggcac gagtccagaa gccgttctac
     9361 ttcatcaacg ccataacgtt cttcgcactc ggtaatatat ttacgggcaa aaatcagata
     9421 atcgacaatc gaactggcgt cggtccaact gcggaataag caattgtttt taaagaaaga
     9481 gttatgtcca tagcaggcgt gtgccatcac cagcgcctgc atagtaatgg tgttctcttc
     9541 catcaagtaa gcgatacacg ggttagagtt aatgacgatt tcataggcca gcccttgctg
     9601 accgtgctta tacagccgtt cagtctcgat aaacttttta ccgaatgacc agtgcggata
     9661 gttaattggc atgccgacgc tggaataggc atccatcatc tgttctgagg ttatcacttc
     9721 aatctggtgc gggtaggtat ccagccggta gagtttcgcc acccggtcta tctctgccag
     9781 ataaacatcc agcaggtcga acgtccagtc gggtccatcg ctcaaacgtg tggtgtcctt
     9841 attcatagaa tcgatcgtcg ccatacgcgc acctcattgt tgtcggcgct ctctgtgtgg
     9901 agcacctcat ttcaagcata gaacacctgt taaaaaccgc gtcgccggag aatttttttc
     9961 tttgcgattt cttattatca gagtgccact aatccgcttc tgaacggaat tttatgctgg
    10021 ataaaaaggg cgttcagcag gagatactaa agacgccata ttgccgcaga gtcagggaga
    10081 tgtgagtcag ctcaccataa aaaagccgta tgttgaataa tattttcaac tgagttatca
    10141 agatgtaatt agattattat tcttttactg tatctaccgt tatcggagtg gctatgcgag
    10201 ttgtcatact gggaagtggt gtggtaggcg ttgccagcgc ctggtactta aatcaggcag
    10261 gacatgaggt caccgtcatt gatcgggagc cgggggccgc cctggaaacc agtgccgcga
    10321 atgccgggca aatctccccc ggatatgctg caccgtgggc agcaccaggt gtgcctttaa
    10381 aagcgattaa atggatgttc cagcgccatg cgccgctggc ggttcgtctc gacggtacgc
    10441 agtttcagtt gaaatggatg tggcaaatgt tacgtaactg cgacaccagc cactacatgg
    10501 aaaacaaagg gcggatggtg cgtctggcgg aatacagccg tgattgcctg aaagcattgc
    10561 gcgccgaaac caatattcag tatgaagggc gtcagggtgg gacgctgcaa ctgttccgta
    10621 ccgaacaaca gtatgaaaat gcgacccgcg atatcgccgt gctggaagat gccggcgtac
    10681 cgtatcagct gctggaatcc agccgcctgg cggaagtgga tcccgcgctg gcagaagtag
    10741 cgcacaaact gacgggcggc ctgcagttac ccaatgatga aaccggagac tgccagctgt
    10801 ttacccagaa tctggcgcgg atggcagagc aggcgggggt taaattccgc tttaatacgc
    10861 ccgttgacca actgctttgc gacggcgagc aaatctacgg cgtgaagttt ggcgatgaag
    10921 tgattaaggc cgatgcgtat gtgatggcgt ttggttctta ctcgacggcg atgctcaaag
    10981 gcattgttga tattccggtg tatccgctga aaggctactc gctgactatt ccaattgcgc
    11041 aggaagatgg tgcgccagta tccaccattc ttgatgaaac ctacaaaatc gccattaccc
    11101 gtttcgataa ccgcattcgt gttggcggaa tggcggaaat tgttggtttt aacaccgagc
    11161 tgttgcaacc gcgtcgtgaa acgctggaaa tggtggttcg cgatctctat ccacgcggcg
    11221 gtcatgtcga gcaggcgact ttctggacgg gtttgcgccc gatgacgcca gacggtacgc
    11281 cggttgtcgg gagtacacgc tttaaaaatc tgtggctgaa taccggtcac ggcacgctcg
    11341 gctggacgat ggcttgcggt tccggtcagt tgttaagcga tctgctctct ggtcgcacgc
    11401 cagcgatccc atatgaggat ctaagcgtag cgcgctacag ccgtggattt acgccatcac
    11461 gtccgggcca tttacatggc gcacacagct aaggaaacga gatgacccgt ccgatacagg
    11521 ccagcctcga tctgcaggca ttaaaacaga atctgtccat tgtccgccag gccgcgccgc
    11581 acgcgcgcgt ctggtcggtg gtaaaagcga acgcttacgg gcatggtatt gagcgtatct
    11641 ggagcgcgct cggggccacc gatggctttg cattacttaa cctggaagag gcaataacgt
    11701 tacgtgagcg cggctggaag gggccgatcc tgatgctgga aggatttttc catgctcagg
    11761 atctggagat ttatgaccag caccgcctga ccacctgcgt acacagcaac tggcagctca
    11821 aagcactgca aaatgcgcgg ctaaaagcac cgttggatat ttatcttaaa gtgaacagtg
    11881 ggatgaatcg gttgggcttc cagtccgatc gcgtgcttac cgtctggcag cagttgcggg
    11941 caatggcgaa tgttggcgaa atgaccctga tgtcgcattt tgctgaagcg gaacatcctg
    12001 atggaatttc cggcgcgatg gcgcgtattg agcaggcggc ggaggggctg gagtgtcggc
    12061 gttcgttgtc caattcggcg gcgactctgt ggcacccgga agcgcatttt gactgggttc
    12121 ggcctggcat tattttgtat ggcgcttcgc cgtccggtca gtggcgtgat atcgccaata
    12181 ccggattacg tccggtgatg acgctaagca gtgagattat tggtgtccag acgctaaaag
    12241 cgggcgagcg tgtgggctac ggcggtcgct atactgcgcg cgatgaacag cgaatcggca
    12301 ttgtcgccgc aggatacgcc gacggttatc cgcgccatgc gcctaccggt gctcctgttt
    12361 tagtggacgg cgtgcgcacc atgacggtgg ggaccgtctc gatggatatg ctggcggttg
    12421 atttaacgcc ttgcccgcag gcggggattg gtacgccggt tgagctgtgg ggcaaggaga
    12481 tcaaaattga tgatgtcgcc gccgctgccg gaacggtggg ctatgagttg atgtgcgcac
    12541 tggcgttacg cgtcccggtt gtgacagtgt aacttgttgt aagccggatc ggaggcagcg
    12601 tcttctggtt gcgaaaaaat catccatccg gctggtcagc aactgtagtt gttaatgtga
    12661 cagagccatt gcccatgata gtgtccatta aaagaatgga cactatttcc ccggaacctg
    12721 aactcgccgc acaggcgttc tacataaaac gcttacgctt cattgttgac tcggagtcga
    12781 cttcagataa atcacgcttc acccttgatg cagattcggg ttcatgttca gattgttgat
    12841 taacaggctg cgagtcctgt tgtctttcca gactcaacag gcccaactgt tgatccagga
    12901 gctccaggtc ctcctgtacc ttcacgcgct ccaggagttc cgggtcatcc aggaactcca
    12961 gatccataag gtcctccagc tcctcgagtc cttccaggtc aacctagcct tccttgtgca
    13021 aaaagtccaa aaggtccttc aggccctgct gttttttcag gccctcgacg gctttgagca
    13081 gcgtcggtaa attgtctttg tcccacgggt taccattaca gtcaaacacc ttcaggctct
    13141 ggggctgcgg cacctgtacc gttttaaact tgttttctcc acagtagtaa attattagtt
    13201 tgggcggcag ccgtggtgaa gagatcaaat cgttactaga acagtcaaat actttcaggc
    13261 gagtcaagtt ctccagcatc tcatcaggta ttgagttaat attgttgccg gcacaggata
    13321 gtttatacag gtactggggc atcgacggcg gtaaaaattg caagaagttt tcagaacagt
    13381 taatcactct caggttagtc gacagctcag gcagccagat aatactgttt tcatagcact
    13441 tcagtaccat cagttttgag ggctgcacct tcggtaacaa gatcaagacg ttatctgtac
    13501 agtcaagcac cagcagccac tttggcatca ccttcggtaa cgcggtcaac acgtttttac
    13561 tgcagtaaaa ttcaatcaga tgtggaggca aattctcagg gagtgaagtc aatcccaggt
    13621 tgctaagatt gagcatactc cgtcttgtaa tgagacattc cttcattctg cgcaatgctt
    13681 ctgcccggtt ttcaatccgg cttgctggca ggttttcttc caggatattt tccttgattt
    13741 cttccagact ttctttcagg tcttcttggg gaatttctga ctgtttgttt tgctggtttt
    13801 cttccaggat ttcgtacagg tttttttgca ggttttcttg caaaccatcc tgctgtatag
    13861 cccattcgca ccatttggcg accatcatga actcgttatg ttgctgattc agacgagtcc
    13921 ccaacgtgat gatggtattg ttgtccacct gtatgttcac tttgcccgcg atactgaggt
    13981 caatgatctg ccagggggca tcaccccagc tgaatttttg cgggctgaga tacttcatgt
    14041 tctgcaaaat ttgattcttg agaagagccg ccctggcggg cgtcgtacgc ctgggttaac
    14101 ggaagatggg ggaatggcgg gggcgaatgg gcagatgtct ttaaagctag ccataatgct
    14161 acgtttcctt gaaataaata tcctctggca gcgccggaga cgtgaatgtg gtcaccgcaa
    14221 aggagccaca ggacgtcatt tgcgctgata tcactactca tcgttgactt ttttcctggt
    14281 cagaaatgcc gtgtcgggtc tggtacatgc tgtattacgg cctaggtagc tctgacgtat
    14341 taaagaaaat acgagagtga acgacccgga aataatcgtt attaagcaaa ggaataacta
    14401 taagaaatcg ctcatgcatc cggtgctaca ggcggatgaa acgcctattg tgtgggctat
    14461 gtcagtgcgg caggttgcgt gggggaatat gttcaggcga tgtcaggcag gggcccctga
    14521 tggttgaccg gtaatgcggg gctggttttt attacctgtt acagtcggaa aaagccctta
    14581 cgctgacttt cttgagccgg tgttttgagt tcattagcca gagcctgcaa tggtttgtca
    14641 tccataattt aaccttcttc tgatgttggg atgagcatat caaaatcagg aaatgacaag
    14701 tttattacag gctcaggctg cgttcagtaa agcatcgcat ccggcgatag atgctgcatt
    14761 aaatttaaga ttcagcttcc tcttcagcca cccgaacgcc aatcttcaac acttcattat
    14821 cttctttctc ggccaccgtc cagatcatcc cggcaaactc tacctggtca ccgacaaccg
    14881 gtgccgcgcc tagcaactgc tggacaattt cacccagcgt ttgctgttta tcacgatatt
    14941 ctcgcccgtc ttccaggcca tatatcagcg caacatcagc atatttggcg ctggcttcga
    15001 gaatgaagtc accaaagaag cgttgatcca gcgcgaccgg tggcgactgg ctgaacagtt
    15061 taccgagcgc cgggagatcg cgttcccgac caattacaca caatacatcg ccttcgcgca
    15121 gtcgggtgct gccggtagga tgaagcaact ggttatcacg aaacagggct gcaatacgcg
    15181 tctcttttgg catatgcaaa tcgcgcagtg ccgcacctac acaccattta tcagcactca
    15241 attgataaac aaactgctcc cacggatttt ccggatgaat atccaggcca acgcgtgaca
    15301 ccggacgtcc gacgggcgga accaccactt tggctttttt cgccgcccac gagagtgatg
    15361 ttccctgcaa gagcagtgaa accagaacca caaagaaggc gacattaaag aacagtcgcg
    15421 cattctccag ccccgccatc atcgggaaca cggctaggat gattggcacc gcgccgcgta
    15481 atcccaccca gctgataaac acgcgctcac gcagattgaa accgcggaaa gggagcaatc
    15541 cagcaaatac cgaaagggga cgggcgaaga atatcatcca tgccgacaaa atgagcgccg
    15601 gaatggcaat aggcagcaga tcgcttgggt taaccaatag ccccagcacc aggaacatgg
    15661 cgatttgcgc cagccaggcg aggccgtcga aattttgcag gatgccgtag cggttgcgaa
    15721 tcggccgatt acccagcaga aaaccgcaca gataaacagc cagaataccg ctgccttcca
    15781 gcgccgtggt taaagcaaaa attaaaatac cgccgcttaa tgccagcaat ggatataatc
    15841 cggcaggcag ggcgatgcga ttaatcattt gcagcagtaa ataaccaccg ccaagaccga
    15901 tgacaatccc gaggccaaat tgctgcagaa tatcgaccac gaacatccaa ctgacactgc
    15961 tttcatgctg ctggatcatt gcaatcaggg taatcgtcag aaagaccgcc attggatcat
    16021 tactgccgga ttcaatttcc agcgtcgagc caacacgttc gttaagcccc ttaccaccca
    16081 gcagagaaaa gaccgctgca gcatcggtgg agccgacgat agcgccgatt aataagcctt
    16141 caatcaaatc aagattaaac agccacgccg ccatcatgcc ggttaaacca gaggtgataa
    16201 gtacgcccag cgtcgccagc gacagcgccg gtcctaacgc cacacgaaag gaactggcct
    16261 gagtgcgcat cccgccgtcg agcaaaataa tcgccagcgc caggttactt accatgtagg
    16321 cgaaggggta attatcaaac gggataccgc cgacgccatc gactcccgcc agcatgccga
    16381 tcgccagaaa aataaccaga ataggaatgc caagacggga agaaaatgaa ctaagtaata
    16441 tactgctggt gacaaggatg gatcctaaga tgaaaaggct aattattgtt gtggcatcca
    16501 atggtcggtt actcctgatt acgctgtctc ttatataaac cctaccatat tagcggcaga
    16561 gacagcgttt tacttagccc tgaagcgtga tttttttaca ttttaagaac aggatgaccg
    16621 ctaatcgtca actgagcgcc ttcccgggtg ttattcagaa tggcatgtgc gcccaacggc
    16681 agcgtaaccg tgcgttgttc atgaccaaaa tcgaggccgg taatgagtgg aatcgacagg
    16741 cgggaacgca aaaacgcgta cactgactcc aggttgtaac ctgcgtcata atcattgggc
    16801 gtgctgccgc taaagctacc gagaataatc gccttctgac gtggcaaaat tcccgcatga
    16861 tagagctgca acagcatacg ttcgacgcgg aaagggtgca cgttaatatc ttccagcacc
    16921 agaataccgt tctcaatttt tggcatccac ggtgtaccaa tcagtgaaat cagcatcgca
    16981 agattgcctc cccacaacgt gccttcggtc tgacatgtcg ggccttcccc ttgccattca
    17041 atggtgaagg tttcattgcg taacgccagc cagaaatggt gctcggtaaa ggcgttcagt
    17101 tcatccgcac caaagtttgc taccagcatg gggccgctaa aggtgatgac attgccttgc
    17161 gccagaagac cgcactgaat ggcggtaaaa tcgctatgtc cgcaaatgag taacgggtca
    17221 tgttgttggc gagccaccag tgcctgccag tcaatatctg ccagtaaacg actggcaccg
    17281 taaccgccgc gtacagccag cacgatggtg ttgggggtag tcagtctggc aagggaattg
    17341 agatcttcca gacgctccgt ttccgtaccc gcaaaacgct cacaacgacg ggcaataacc
    17401 tcgacgttat tgacttgatg ccccgcgtcg gtaaggcgct ggataccacg cagcgcggcg
    17461 tgctgtttaa tgctgtaacc cgatggggca attaagtgaa acagagacat ggcaattcct
    17521 tgctgacaac agaaacgaaa tgtatatcat gccgcttagg tgtgccgttg tcacctcaac
    17581 ggcgattcca ggctataagg atagaagaag tgaaattgag atggtttgcc tttttgattg
    17641 tgttattagc gggttgttca tcaaagcatg actatacgaa cccgccgtgg aacgcgaaag
    17701 ttccggtgca acgtgcgatg cagtggatgc caataagcca gaaagccggt gcagcctggg
    17761 gcgtcgatcc acaattgatc acggcgatta tcgctatcga atcgggtggt aatccgaacg
    17821 cggtgagtaa atcgaatgcc attggtttga tgcagttaaa agcttcaacc tccggacgtg
    17881 atgtttatcg ccgtatgggc tggagtggtg agccgacgac cagcgagctg aaaaatccag
    17941 agcgtaatat ttcaatgggg gcggcttacc tgaatattct ggaaaccggc ccgctggcag
    18001 gcattgaaga tccgaaggta ctgcaatatg cgctggtggt gtcatacgct aacggggcag
    18061 gtgcgctgct acggactttc tcgtcagatc ggaaaaaggc gatcagcaaa atcaacgatt
    18121 tggatgctga cgagttcctc gatcacgtag cgcgaaatca tcctgcgccg caggctccgc
    18181 gctatatcta caaacttgag caggcactgg acgcgatgta aatcagtcgc gcactttgtc
    18241 cgctttttcc cgggcttctc gctcgagaga gaaaataatc cgctgtaatt gccgctccac
    18301 cgtcgggctg acgttaagaa aacggaagct cagacgggga gtggtgatgg tttcattctt
    18361 gccatcaatc actttgcgct cgctggtgga gattaactgg gcgtcaaagt gaaaaacacc
    18421 ccattgcccc atgttgactt caatttgagc gaagcgcata ccttcttgta attcggcagg
    18481 ctttgctgtt tccagtaatg cgcccatgcc gcctaacgac aaatcataca agcggaaacg
    18541 taacgtactg ttatccgcca gtttggtctg gcaaaaataa ggcggatgga gtggggcgga
    18601 gatgcggaaa tatcggcgtc gttgtacaaa ccataaggtg ggaggcggta cggtaataaa
    18661 tgccggaagc tgcaagtatt cactctgctg tagttgttca acagtaaact cgactttcgc
    18721 accctgagtt tcggcggtaa tggtaatgtg ctgtgccttt agcacggcga tgttgtcttc
    18781 ggcttgactg ccgaaatcca gcaccagttt atccggggtt attgccagta ttttgctgat
    18841 cagctgcccg ccattccaac tgagacgcaa aggaattgcg gctttgtgca aatcgcgtaa
    18901 cacgcccagg acggctaacg gattttgttt caggaactgc tcatggtaat gactcacgcg
    18961 gaaaaactcc tgactgacaa actgtctttg gtttatcggt catagttaac aaaacttaat
    19021 acaaatgcgt gaatattttt tacatgttgt tcttaaatca gccgacaaac gcccagcatc
    19081 gatcccgccc ttgcctatac ttagagcgtt gacgtaagca tttcttgcga ccgattcatc
    19141 gaaacgaggg cctgaacatg ggaattattg cctggattat ttttggcctg atagccggca
    19201 ttatcggcaa gctaatcatg ccggggcgtg atggtggtgg atttttcctg acctgtattc
    19261 tcgggatagt cggtgcggtg gtcggcggct ggctggcgac catgtttggc attgacggct
    19321 ccatcagcgg ttttaatttg cacagcttcc tggtggcggt ggtgggcgct atcctggtgc
    19381 tgggcgtatt ccgcctcctg cgaagagaat aagattttca taaggcggat agcgatacag
    19441 atgccgctat ccgctttcac atcagaacgt atactcgaca cctgcccaga aattgcgacc
    19501 ttcttcaata aacccttcac tgtaggcata ttgggtatca aacaggttat tgaccgacgc
    19561 attgacgctg aagccatgac ctaaggtgta atcggctcga atgtgggtca ccgcaaaacc
    19621 ggcggctttt tgtgaaccgt cactgttgct gtagctggag gaacgcgcct cttccgacag
    19681 cgttacgctt aacggctccc atggtttgag tgtcatccat gcggtcattg tctgcgttgg
    19741 aagatcggtt atcttgccga tgtctttgcg tttggcgtca gcgtggatca gagcgtagct
    19801 caatcctaca tccagtatat tgctgatttt ccccttaata ccggcatcca gaccgctgta
    19861 atccaccgtg ccgctgttct gattttgaat ggtatcggca ttgatattgt gcgagaggat
    19921 ggcatcactc acccggttat agtaaacgct gacctcaaat ccccagtcgc gcgtgaaggc
    19981 accattccag gttaaatcca ccccgcgagc gcgttccggc ttgagctgcg ggttaacaat
    20041 cgctatctgg ttatacgcag gtttggatgt ggtatagcgt tctttcagcg tcggaaagcg
    20101 tgtgcggtca tagtacgaaa gcgccagagt gtcttcattg gcaaagtggt atttccccat
    20161 cacctgccag ttaaaagctg actgattgtt gtcgtcataa tgggtgatgc tgccatcttt
    20221 ctcgtgtttt ttcgcttcta cgctatcgcg ccagtcatag ctgattcccg ccacgacatc
    20281 gacattatca gcagcagccc attgatattc actggcgagc gaccaggtac gatcttcata
    20341 gcgatcgtaa gcggcgtgcg gcgcaccttt ttcccggtgt acgtcatctt tccagttaac
    20401 ggcaaacgac agcagatcgt tttcacgcac atcggctgcc agttgtaatc cggcaccgtc
    20461 gctgtaatcg gaataatggc tgtagctgcc ttttttattt ttcaaatcag ccagcgagtt
    20521 gtacatcatc agcgtatttt caaaggtgtc gcgatacagc cgacttttca gggtaaaacg
    20581 gtcgtttagt tgggtcgctc cctgataata aaaactttct ttgtcatact ctggccactg
    20641 ccagtagcgt gatttttgac cacaatttcc gctgtatggc gggttatctt tttcaccatc
    20701 ctgcttaatg taagtcagcg tgtattcatc gttttcgcgc ggtgtaaaac cgagcttcac
    20761 aatgccgcgt ttatcatcag ccgatgaatt aatcatcttg ccgtgtttgc ctgcaatatc
    20821 attatttaca ccatgcggca ggccgagaaa atcctgcttt agctggctac cgctgacttg
    20881 caaataccca agatcgctgc tggcggcaaa tgaagcatgc atatcgtagg cattgtcctg
    20941 actacggctc catccctggc gatatcccag acttgcttct tcagggtggt gatattaatg
    21001 gctccgccca tctgattagg cccatgaagc agcgacgaat accctttgga aacttcgact
    21061 gcccccagat tgttggtcag aatccgcgcc agatcgaggt tgccgtcata ggggacataa
    21121 atgggcacac cgtcgaaata gaccggcacc tgccgactat caaagccacg aacttttacc
    21181 tgttcttcgt tgcggctgcc tgacttttgc agcaccacgc cggggacgac acttaatgcc
    21241 tgagcgacat tctgtttatc caaggccttc atggtgggtt gatcgagaac ggtggtcgtc
    21301 gtcgatgata ccggactgga ccatacggtc agcgtgtcgc tctcctgagc cgctacggcc
    21361 tgctgggtaa aggcaagtga taatgctgtg cagagataac gttttttgag tctcatagaa
    21421 taaattccgt ttaacaaaga attaaataga ggctgtgggt tgcagactga ctttaatgtc
    21481 agctggtgcc gcgtaataag gcgcggaggt aataaaaagg cgaatgccgc agtcgaggta
    21541 atttttgagt gtcgtcagat taattccgcc ggtcagagcc agcgtgcagt gaggagccag
    21601 cgacggcgct atttgcgcaa tttcagttgc ctgttgcgga ctaaatttgt cgagctgaag
    21661 tacgtctggt tgtgcgcgta acgcggcgat agcctctttc ggcgtgtcgg cttcaacaac
    21721 aattttcttc tcgggtgcgt ggcgacgtaa ctgagcgatt gcgcctgacc agccctgatt
    21781 gtcatgaaga aaatggcggt ggttggcaaa caataatatg gtttccgcac atccggcacg
    21841 atgaattagt cctccggcag ccagaattgc ctgcgaagcc agtaagcggg tgcccggaat
    21901 tgcttttcgg gtgcaggcga tattgccgtc aggataacgt tcacgaagca atgccagcat
    21961 ttgagcgaga taatcagaaa caccgcaact ccactccagc acattctgga ctgccttcca
    22021 tccctgatga agtgctgcgg cattaccctg cgcacggatt agccgttgac ccgcgttcgc
    22081 ttgtgaaccg tcgctgaccg cgtcatcaat agttaatccc agcgtagtta acatcttaca
    22141 cgcgacagaa acgccgctga cgcagccgcc ctgacggtga aaaaacgcta tatagccagg
    22201 ctggtgtcca atatttaatg ctcgcgtggt caggtcacct ccctggatat cttccagcag
    22261 taacgcatcg atttgtgcct gagaaaggaa aatcatcaca gtgcctcctg cggtacgcta
    22321 tcccaccaga tgagcgccca gtcgcggctg gcaggggcga gggcacgagc atcttgttgt
    22381 tgataccagt gatataaccg ctcacgctca tcgtcgttaa gcgcgccaag actccagctg
    22441 acgttctgct caaaacgttc ccaggtgctg ttatcctgtt ggcaattttg tccacggata
    22501 aaatcaacgt gggcataaat tcccatctga tacaggacgt tgagcgcaaa gatatagttg
    22561 ggtaattcga tcacttcccg accagccgcc cgctgaatgg ctggagagac gaacgaggtg
    22621 ctgaccagat gagttgtata gacgcgaagc cgtgcctgat tattcagttt gctcattgca
    22681 tggcgcatat cagccaccag agtggagcgg gaagccacgg caatatcgca gcgtggcagg
    22741 tcactccagt cctcttccca ggcgcgctgg atccagtgga cattgtcagc cttcagtgcg
    22801 gcggcgcggc gggcggcaac gttcagcatc ccctgactgt aatcaacgcc ataaaccgta
    22861 gtgagtttgt cagccagcgc cagcgatacg gtgcctggtc cgcagcccat atcgaataag
    22921 gtctgagcgc cttgcaggtc aattttagcg atcagttgtt gcaggtagct gtccgttggc
    22981 gaggcgcaat tttccgccat tttctccgcc cgttgatccc agtgatcggg cgttttttct
    23041 gtgcggtgag ccagtttgag ttgctgcaga taaaggtctg caaaatcaat atcatcaatg
    23101 agcatgattg ttccttagcg gctaattgca gagaggtgat gatgaatttg gtcggcagaa
    23161 acccgataca gggccgccag tttgttcgtt gttaactgtt cggtcggtaa gccttgtgtt
    23221 actcgtccat ctggctctac ttggataatg ctgtcggcaa tggcgttggc atggagcgta
    23281 tggtgagtcg acattaacat tgtcatgccg ttatttttga gctgcgccag cgtgtccaga
    23341 agttggatct gatgaccgaa atcgaggctg gaggcgggct catccagtaa caacaagcgc
    23401 ggttgttgga cgagtgcacg ggcgattaaa gccagctgac gttcaccgcc gctaagcgta
    23461 ttccagcgtc gtgtcgccag atgcaaaatg cccagctttt ccagttgctc cgtggctttc
    23521 aggcgttctt gcttaccggg aacggaaaaa gcgcctatgg tgggggccag gcccatcaac
    23581 accatatcca gcacactgaa agcaaaaata ccgtcgtgcg cctgcggcac ccaggcaata
    23641 gcctgtgcgc gctggcgatg gctgagtgtc tgcacagaga ggctatccag taggatctcg
    23701 ccttttaata acggtataac gccaagaatg gagcgcatga gtgtggtttt gccgcaacca
    23761 ttcgcgccaa gcagacaaca aattgttcct tgcgacaggg tgaaggacac gtcgcgtagc
    23821 actggatggc gggagtaacc cagagacgca tgacgaacgg caagcaaagt cactggcgac
    23881 ctccgcgtaa caacaaaagc aggaaaaacg gtgcgccaac aaaagcggtc agaatgccaa
    23941 gtgggatttc tgttgttcca atgctgcgcg ccagcgtatc ggtaagcagg agcagaatag
    24001 cgccagtaca cattgccata ggcaacagct gctgatggtt atgaccggtc aacaagcggg
    24061 caatgtgcgg aacgaccaga ccgatccaac caattatgcc cgctattgct accgtgctgg
    24121 cggtgattag tgtggcgcag acaatcaatc caaagcgcag acgcgtcacg ttcagtccca
    24181 gcgaacgggc ttcgtcgtcg gaaagcgtga gtaaattcat gcgccagcgg agaaaaaaca
    24241 ggggcaagga gcctatgaga atgatcggtg cagcatagca gagatcgcgt aaggtcacgg
    24301 tggacagtcc ccccagcaac cagaaggtaa ttgacggtaa ctgggtgtaa ggatcggcca
    24361 gcgttttaat tagcgatatc cccgctccgc acaacgttcc taacgcaatg cctaccagca
    24421 ccagggttaa gatcggatcg tggcgggtca cccgacgcgt aatcaaccag acgcccgcga
    24481 ccaccatcag cccgccgcaa aatgcgtaca gctggatgta aacaatgggt aatccccaca
    24541 ggattgccgc gcaggcacca agtccggcac cggcggagac gcctaaaata tccggtgaaa
    24601 ctaagggatt acgcaacatt ccctgatagg tggttccggc tcctgccagc gctgcgccca
    24661 gtaacagcgc agcaagaata cgcggcacgc ggatttgcca gaagacaatt tgttcctgca
    24721 cagactcatt ttcttgacga agaattagcg ccagcagttg ctgcatatcg agatgataag
    24781 ccccacttac ggcggcaaaa gtgatactga tagctgccag cagcaaaata catccgttta
    24841 cgattctcat tagctcgcca ccaatttttg atagtcggca tcactgagtg aacaatgcca
    24901 gaacagttgg gcgtaatgct gcatgtcact tttaaactgg cgattgatgg cgggatccag
    24961 ccaggcgtga agtctgcgta atcccagaag acggttgatt cccggcgggg catcgagcca
    25021 gccaaagggc aagccactta aaaataagat gcgttgttcc gcaacggctt tcacgccctg
    25081 ccagagagga tcacgacgaa taaaatctgc agtaacggcc tcctgaacca gaataatatc
    25141 cggctgccag cgcaggagat tttccatgga aacctgtgtc aggccgtggc gatcggctat
    25201 ttgcgcgacg ttgtgcaaac ctaataattc cgccgcctcg gtatgcaacg aaccctgtaa
    25261 gcccgtttcc agccctcggg gaccgcgcgc agcataaaag cggaggttag cggcgggtga
    25321 ggtggcgaat gcctgagctt caccaacgaa gtggctggcg agattggctt gttctgcggc
    25381 gcggtgctct tcgcctaacg ttttgccaag cgttgttaac tgttctgctg attgcgccag
    25441 ttttccgtta agcaataacc aggggatttg tgtctgttcg ctaacctgcc gtgcctggga
    25501 gatcaaggtt tcatccgtat tgccgcaatc aacgaccaaa tcgggatgta acgccataag
    25561 cccttccagc gatagtgtgc tggcgcgccc ggcgagtcgt cccagcctgg gtaactggcg
    25621 aatgtgctct ggcaatggaa ttaatgcctg acgggcaaaa tcaaaagagg aaaaaccgac
    25681 cattttttct ggcgcgacag ccagcaagag caaatcggcg ggcgcgcctg cgctgacgat
    25741 gcgtgtaatg ttttgggctt cgggaagaga ttgcgaagca aaacgattaa ctgtttgtga
    25801 aaaggacggt agagactgca ggagcagggt agatgcaagt gcctgcgcaa acgtccgtct
    25861 ggttatgggc atggtgaaca tcctgaaaag taaaatcgat atatactttc atatatatcg
    25921 ataaaaatca tgttcaatat caaagtttag ctattgaaaa catgaagatg tgacgacttc
    25981 cgcaagcctg ttgaccctct ctatcattaa aaatgtattt atcaggtggc taatattatt
    26041 taaatcattt aaggctaaat tatttaaata tgaatgacat tattgttttt ttatcgtcca
    26101 cggttacgcg acgttgaagt tacttgtttt ataatgagcg atttttgata tctcgccgag
    26161 aaaattatca gcattctctc gtttcagtgt cgtgaatata ctgttaacgg aggtgaaaag
    26221 aatgctgggg gttatacaaa acctatgttg ttgtcgtccg gattcaccgc ttgctgatag
    26281 taagcagcgt aaccagaagt taccgcaaca agactttgag gattttaaga acgacgttat
    26341 taatgtcatt aacaacaatg aagatattta ttttcttaga gagagtaatt tactgaatgc
    26401 gtttattcgg acgtgcaaaa aaagagatga taacagttac ggaaatatga tatttacgct
    26461 ttattttgat aacgatatct atgggaacag aattatcaaa gcatcgctga acggcggaga
    26521 gttgcagtta ttaagcacag ggtgtagcaa tgataacatt ctc
//
LOCUS       DAZPAB010000036        26184 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZPAB010000036 DAZPAB010000000
VERSION     DAZPAB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.036"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..213)
                     /locus_tag="ACHUPG_001370"
     CDS             complement(<1..213)
                     /locus_tag="ACHUPG_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HFR0861047.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="ACHUPG_001371"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="ACHUPG_001371"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR0861048.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="ACHUPG_001372"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="ACHUPG_001372"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR0861049.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="ACHUPG_001373"
     CDS             2894..3634
                     /locus_tag="ACHUPG_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0861050.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="ACHUPG_001374"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="ACHUPG_001374"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR0861051.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4192..4794)
                     /locus_tag="ACHUPG_001375"
     CDS             complement(4192..4794)
                     /locus_tag="ACHUPG_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR0861052.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4864..4929
                     /gene="yobI"
                     /locus_tag="ACHUPG_001376"
     CDS             4864..4929
                     /gene="yobI"
                     /locus_tag="ACHUPG_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFR0861053.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5068..5679
                     /gene="ruvA"
                     /locus_tag="ACHUPG_001377"
     CDS             5068..5679
                     /gene="ruvA"
                     /locus_tag="ACHUPG_001377"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR0861054.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5688..6698
                     /gene="ruvB"
                     /locus_tag="ACHUPG_001378"
     CDS             5688..6698
                     /gene="ruvB"
                     /locus_tag="ACHUPG_001378"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR0861055.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6845..7630)
                     /gene="znuB"
                     /locus_tag="ACHUPG_001379"
     CDS             complement(6845..7630)
                     /gene="znuB"
                     /locus_tag="ACHUPG_001379"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR0861056.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7627..8382)
                     /gene="znuC"
                     /locus_tag="ACHUPG_001380"
     CDS             complement(7627..8382)
                     /gene="znuC"
                     /locus_tag="ACHUPG_001380"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR0861057.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8461..9393
                     /gene="znuA"
                     /locus_tag="ACHUPG_001381"
     CDS             8461..9393
                     /gene="znuA"
                     /locus_tag="ACHUPG_001381"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR0861058.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9409..10731
                     /gene="mepM"
                     /locus_tag="ACHUPG_001382"
     CDS             9409..10731
                     /gene="mepM"
                     /locus_tag="ACHUPG_001382"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR0861059.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10851..11822
                     /gene="lpxM"
                     /locus_tag="ACHUPG_001383"
                     /gene_synonym="msbB"
     CDS             10851..11822
                     /gene="lpxM"
                     /locus_tag="ACHUPG_001383"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR0861060.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11953..13395)
                     /gene="pyk"
                     /locus_tag="ACHUPG_001384"
     CDS             complement(11953..13395)
                     /gene="pyk"
                     /locus_tag="ACHUPG_001384"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR0861061.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13523..14392)
                     /gene="hexR"
                     /locus_tag="ACHUPG_001385"
                     /gene_synonym="yebK"
     CDS             complement(13523..14392)
                     /gene="hexR"
                     /locus_tag="ACHUPG_001385"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR0861062.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14729..16204
                     /gene="zwf"
                     /locus_tag="ACHUPG_001386"
     CDS             14729..16204
                     /gene="zwf"
                     /locus_tag="ACHUPG_001386"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR0861063.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16439..18250
                     /gene="edd"
                     /locus_tag="ACHUPG_001387"
     CDS             16439..18250
                     /gene="edd"
                     /locus_tag="ACHUPG_001387"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR0861064.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18287..18928
                     /gene="kdgA"
                     /locus_tag="ACHUPG_001388"
                     /gene_synonym="eda"
     CDS             18287..18928
                     /gene="kdgA"
                     /locus_tag="ACHUPG_001388"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR0861065.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(18984..20162)
                     /gene="purT"
                     /locus_tag="ACHUPG_001389"
     CDS             complement(18984..20162)
                     /gene="purT"
                     /locus_tag="ACHUPG_001389"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR0861066.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20296..20586
                     /gene="yebG"
                     /locus_tag="ACHUPG_001390"
                     /pseudo
     CDS             20296..20586
                     /gene="yebG"
                     /locus_tag="ACHUPG_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            20653..21009
                     /gene="yebF"
                     /locus_tag="ACHUPG_001391"
     CDS             20653..21009
                     /gene="yebF"
                     /locus_tag="ACHUPG_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR0861067.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            21336..21995
                     /locus_tag="ACHUPG_001392"
     CDS             21336..21995
                     /locus_tag="ACHUPG_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR0861068.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22205..24265
                     /gene="ptrB"
                     /locus_tag="ACHUPG_001393"
     CDS             22205..24265
                     /gene="ptrB"
                     /locus_tag="ACHUPG_001393"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR0861069.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(24262..24924)
                     /gene="exoX"
                     /locus_tag="ACHUPG_001394"
     CDS             complement(24262..24924)
                     /gene="exoX"
                     /locus_tag="ACHUPG_001394"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR0861070.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24948..25604)
                     /gene="yobB"
                     /locus_tag="ACHUPG_001395"
                     /pseudo
     CDS             complement(24948..25604)
                     /gene="yobB"
                     /locus_tag="ACHUPG_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
     gene            complement(25706..25936)
                     /gene="holE"
                     /locus_tag="ACHUPG_001396"
     CDS             complement(25706..25936)
                     /gene="holE"
                     /locus_tag="ACHUPG_001396"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR0861071.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26075..>26184
                     /locus_tag="ACHUPG_001397"
     CDS             26075..>26184
                     /locus_tag="ACHUPG_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861072.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAA"
BASE COUNT         6652 a         6485 c         6818 g         6229 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcaa cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cagcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagat atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggttaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtcct acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctga cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tccggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga
     4441 acagaactgg cgcgaagatt cgtatttaaa acccgtgtgg taaagtttgc gtagccggga
     4501 gggaacctgt tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt
     4561 tatagcatag cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga
     4621 aaggggaacc cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc
     4681 gacgtgatta ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat
     4741 acatgtaaag acgatatctc caatttcata ttcagcaggg taattaatat tcacgttgtc
     4801 attccattga aatagataca aatatacgta atagcataaa aaaattattt ttcaggagtc
     4861 gcaatgtata tttttattac gcatttcttc actgaatatg taatattcaa atatttgctt
     4921 ccaatataac ctgttgaata aattatactg tgccattttt cagatcatcg ggacacctcg
     4981 caagttttct tcatccttcg ctggatatct atccagcatt tttttatcat acagcattat
     5041 ctttgattca ttacgcagga gcgtcatgtg ataggcagac tcagaggcat catcattgaa
     5101 aaacaacccc cgctggtgtt aattgaagtg ggcggcgtag gctatgaagt gcatatgccg
     5161 atgacctgtt tttatgaact ccctgaagcg ggtcaggaag cgatcgtttt cacccacttt
     5221 gtggtgcgtg aagacgcgca actgctgtac ggttttaaca ataaacaaga acgcacgttg
     5281 ttcaaagagt tgatcaaaac caacggcgtc ggcccgaagt tggccctggc gatcctctcc
     5341 ggaatgtcag cgcagcagtt cgttaatgcc gttgagcgtg aagaagtggg ggcactggtg
     5401 aaactgccgg gtattggcaa aaaaaccgcc gaacgcttga ttgttgaaat gaaagaccga
     5461 tttaaaggtt tgcatggcga tctctttacg ccagccgccg acctggtact cacgtctcct
     5521 gctagcccgg cgaccgacga tgctgaacaa gaagcagttg ccgcgctggt ggcgctaggc
     5581 tataaaccac aagaagcaag ccgcatggtg agcaaaatcg ctcgccctga caccagcagt
     5641 gaaacattaa ttcgcgaagc cctacgcgcc gcgttatgag gtaaaggatg attgaagcag
     5701 accgtctgat ttctgccggt accactttgc cggaagatgt agcagatcgc gccattcgcc
     5761 ctaaattact ggaagagtat gttggtcagc cgcaggttcg ttcacagatg gagattttca
     5821 tcaaagcagc gaaactgcgc ggcgatgccc tcgatcattt gttgattttt ggtcctccgg
     5881 ggttgggtaa aactacgctt gccaacattg tcgccaatga aatgggcgtt aatttacgca
     5941 cgacttctgg tccggtgctg gaaaaggcgg gcgatttggc tgcgatgctc actaaccttg
     6001 aaccgcatga cgtgctgttt attgatgaga tccaccgttt atcgccagtt gttgaagaag
     6061 tgctgtaccc ggcaatggaa gactaccaac tggatatcat gattggtgaa ggtccggcgg
     6121 cacgctccat taaaattgat ttgccgccgt ttaccctgat tggcgcaacc acgcgcgcag
     6181 gttcgctgac atcaccgttg cgcgaccgtt ttggtattgt gcaacgtctg gagttttatc
     6241 aggtgccgga tctgcaatat atcgtcagtc gcagtgcacg ctttatgggg cttgagatga
     6301 gtgatgacgg tgcgcaggaa gtagctcgtc gcgctcgcgg tacgccacgt attgccaacc
     6361 gtctgctgcg tcgagtgcgt gatttcgccg aagtgaagca cgatggcacc atctcggcag
     6421 atatcgctgc tcaggcgctg gatatgttga atgtcgatgc tgaaggtttc gattatatgg
     6481 accgcaaatt gttgctggcg gtaatcgata agttctttgg tgggccggta ggtctggata
     6541 acctggcggc agccattggc gaagaacgtg aaaccattga agatgtactg gaaccttatt
     6601 tgattcagca aggctttttg cagcgtacac cgcgcgggcg tatggcgacg acgcgggcgt
     6661 ggaatcactt tggcataacg ccgccagaaa tgccgtaagt cggattggtc gctcgcgccg
     6721 catccgacac tggcagtgtg tctgatgcga cactgttcgc gtcttatcag gccagccaga
     6781 gatagcgaac cgtaggtcgg ataaggcgct cgcgccgcat ccgacaaatg tgttcagcga
     6841 tagattagct ggcctgcttt ttcatcatac tgataataaa taacagtgcc gcacatagca
     6901 ccaccgacgg acccgccggc gtatcgtaaa acgcggaaaa ggttaaaccg ccagttactg
     6961 ccaccatccc caccaaaaca gcgacaccag ccatctgttc cggcgtacgg gcaaagcgac
     7021 gcgcagtagc tgcaggaata atcagcaacg aagtaataat caacgcaccg acaaatttca
     7081 tcgctacacc aatcgtcaat gccgtcacca gcatcaacaa caatttcact tgctgtaatt
     7141 tcacaccatc aacaaacgcc agatccgggc taatcgtcat cgacagcaaa ttgcgccatt
     7201 gccagaacaa aatagccacc acgatgacca cgccaatcgc aatagagatg agatcttctg
     7261 gcgtcactgc cagtaaatca ccgaacaggt aagccatcaa atcaacacga atgttagaca
     7321 tcagactaac gaccactagg cccagcgaca gggcactgtg cgccataatc cctaataacg
     7381 tgtcgatcgc cagctgtgga cgcttctcca gccataccag accgcccgcc agcagcagcg
     7441 taacggcaat caccgcatag aatggattca cgtccagcaa caaaccaaac gcgacaccaa
     7501 gtaatgaggc atgagccagc gtatcaccga aataagacat acgacgccag actacaaacg
     7561 aacccagcgg acccgcggca caggcgagca tgatcccggc taaccaaccg ggaaataata
     7621 attcaatcat gagcgatcat ttccccgacg caaaacaatt cgtccctgta aatcgtgacg
     7681 atgattatga tgatggcgat agatacccag ttgttcagca ccacgaggac caaacattga
     7741 aataaactcc ggatgcaggg aaacaacttc cggtgtgccg gaacaacaaa tgtggtgatt
     7801 caggcacagc acttcatcgg tttttgccat caccagatgc agatcgtgag aaaccattaa
     7861 aacgccacaa tccagttcac gacgcaactg gtcaataagg tcatataacg ccacttggcc
     7921 atttacatcc acgccttgag tgggttcatc cagcactaat aattgcgggc aatttaacaa
     7981 tgctcgcgct aatagtacac gctgcgtttc gccaccagag agcttttgca tcggtgcgtt
     8041 aatcaggtgc ccggcctgga cacgtttcag tgcaggcaaa atatcttctt tatgtgtacc
     8101 agggcgtaag cgtaaaaaac ggtttacggt cagtggcaac gtggtgtcga gatacagctt
     8161 ctgcggtaca tagccgatgc gcagttttcc gttgcgcttg ataacccctt catcgggtgt
     8221 taccagcccg agcactaccc gtaccagtgt cgacttacct gcgccatttg gcccaagtaa
     8281 agtcaaaatt tttccaggtt taagttccag cgacacatca gagaggacgc ggcgttggcc
     8341 aaaagaaacc gagacatttt ccagggaaac cagacttgtc atgttaattt tagtcttgca
     8401 gtagtcatga aatgttataa tatcacactt ctcatattca ttacgatgat tggtcgcatt
     8461 atgttacata aaaaaacgct tcttttcgca gcattatccg ccgctctctg gggaggtgca
     8521 acacaggccg cagatgccgc cgttgtcgct tcgcttaaac ccgttgggtt catcgcttct
     8581 gccattgctg atggtgtaac agaaacggag gttttactgc ctgacggcgc ttcagaacat
     8641 gattattcac tgcgtccatc ggatgtaaaa cgcttacaga acgcggactt agtcgtttgg
     8701 gttggcccgg agatggaagc gtttatgcaa aaaccggtaa gcaaattacc agaagcgaag
     8761 caggtaacga ttgcgcagct tgaagatgtg aaaccgctac tgatgaaaag tattcacggc
     8821 gatgatgatg atcacgacca cgcggaaaaa agtgacgaag atcaccatca cggcgatttc
     8881 aacatgcatc tttggctttc cccagagata gcgcgggcta cagcggttgc aatccatgga
     8941 aaattagtgg aacttatgcc gcaaagtcga gccaaacttg acgccaacct gaaggatttt
     9001 gaggcacaat tggcctcaac cgaaacgcag gttggtaacg agctcgcgcc gctcaagggg
     9061 aaaggttatt tcgtttttca cgatgcttac ggctatttcg aaaaacagtt cggactgaca
     9121 ccgcttggtc attttaccgt taaccctgag attcaacctg gcgcgcagcg tttacatgaa
     9181 ataagaacac agttggttga gcaaaaagca acctgcgttt ttgctgagcc acagttcagg
     9241 ccagcggtcg ttgagagcgt cgcacgaggg acatccgttc gtatgggaac gttggatccc
     9301 ctggggacga atatcaaact gggtaaaaca agctattcag aattcctgag tcaattagcc
     9361 aaccagtatg cgagctgcct gaaaggagat taatgaggaa gtgattacgt gcaacagata
     9421 gcccgctctg tcgccctggc gtttaataat ttaccgcgac cacaccgcgt tatgttgggg
     9481 tcgctcaccg ttcttactct ggccgtcgct gtctggcggc cctatgttta tcaccgtgat
     9541 gccacgccaa ttgtcaaaac cattgagctg gaacagaacg aaattcgttc gctcttacct
     9601 gaagccagtg agccgattga tcaagctgca caagaagatg aagccattcc ccaggacgaa
     9661 ctggatgaca aaatcgccgg tgaagcgggc gtgcatgaat atgttgtttc cactggcgat
     9721 acgctaagca gcattctcaa tcagtatggt attgatatgg gtgatatcac ccaacttgct
     9781 gctgccgaca aagaattgcg taacctgaaa atcggtcaac aactctcctg gacattaacc
     9841 gcggacggcg aactgcagcg cctcacctgg gaagtgtctc gtcgtgaaac ccgaacctat
     9901 gaccgtactg ccgctaacgg ttttaaaatg accagcgaaa tgcagcaagg agagtgggtt
     9961 aacaatctgc tgaaaggtac cgtcggaggg agctttgttg ccagcgccag aaacgccggt
    10021 ttaaccagcg ccgaagtgag cgcagtgatt aaagccatgc agtggcaaat ggatttccgc
    10081 aaactgaaaa aaggcgatga atttgcggta ttaatgtctc gagaaatgct tgatggtaaa
    10141 cgtgagcaaa gccagctgct gggcgtacgt ttgcgttcag aaggtaaaga ttattacgca
    10201 atccgcgctg aggatggcaa attctacgat cgtaacggta ctggtctggc gaaaggattc
    10261 ttgcgattcc cgacggcgaa acagttccgt atctcgtcta actttaaccc gcgtcgaact
    10321 aatccggtga caggtcgcgt tgcaccacac agaggtgttg atttcgccat gccacaaggt
    10381 acgccagtgc tttcagtggg tgacggtgaa gtggtggttg ccaaacgcag tggcgcagca
    10441 ggttattatg tggctattcg tcatggtcgc agctacacca cgcgttatat gcacttgcgc
    10501 aagattctgg tgaaaccggg acagaaggtg aaacgtggcg accgtatcgc gctttccggt
    10561 aataccggac gttcaactgg gccgcatctg cactatgaag tatggataaa ccagcaggcc
    10621 gtaaacccgc tgacggcaaa actgccgcgt accgaagggc tgaccggctc cgatcgtcgc
    10681 gaattcctgg cgcaggccaa agagattgtg ccgcagctac ggtttgatta attaacatcc
    10741 attcgcagcc ggtacgcagt cagtaccggc tttttttatt tggtgcgggg caagttgtgc
    10801 cgctacacta tcaccagatt gatttttgcc ttatccgaaa ctggaaaagc atggaaacga
    10861 aaaaaaataa tagcgaatac attcctgagt ttgataaatc ctttcgccac ccgcgctact
    10921 ggggagcatg gctgggtgta gcagcgatgg cgggtatcgc tttaacgccg ccaaagttcc
    10981 gtgatcccat tctggcacgg ctgggacgtt ttgccggacg actgggaaaa agctcacgcc
    11041 gtcgtgcgtt aatcaatctg tcgctctgct ttccagaacg tagtgaagct gaacgcgaag
    11101 cgattgttga tgagatgttt gccaccgcgc cgcaagcgat ggcaatgatg gctgagttgg
    11161 caatacgcgg gccggagaaa attcagccgc gcgttggctg gcaagggctg gagatcatcg
    11221 aagagatgcg gcgtaataac gagaaagtta tctttctggt gccgcacggt tgggccgtcg
    11281 atattcctgc catgctgatg gcctcgcaag ggcagaaaat ggcagcgatg ttccataatc
    11341 agggcaaccc ggtttttgat tatgtctgga acacggtgcg tcgtcgcttt ggcggtcgtc
    11401 tgcatgcgag aaatgacggt attaaaccat tcatccagtc ggtacgtcag gggtactggg
    11461 gatattattt acccgatcag gatcatggcc cagagcacag cgaatttgtg gatttctttg
    11521 ccacctataa agcgacgttg cccgcgattg gtcgtttgat gaaagtatgc cgtgcgcgcg
    11581 ttgtaccgct gtttccgatt tatgatggca agacgcatcg tctgacgatt caggtgcgcc
    11641 caccgatgga tgatctgtta gaggcggatg atcatacgat tgagcggcgg atgaatgaag
    11701 aagtcgagat ttttgttggt ccgcgaccag aacaatacac ctggatacta aaattgctga
    11761 aaactcgcaa accgggcgaa atccagccgt ataagcgcaa agatctttat cccatcaaat
    11821 aaaaaagcct ctcgcgagga gaggccttcg cctgatgata agttcaagtt tgcttcagaa
    11881 tattcgaaat ctgttgaact atcattgaac tataggccgg atgtggcgtt ttcgccgcat
    11941 ccggcaacgt acttactcta ccgttaaaat acgcgtggta ttagtagaac ccacggtact
    12001 catcacgtcg ccttgggtga caatcaccag gtcaccagac atcaagtaac ctttatcgcg
    12061 cagcagatta accgcttcgc tggcagctgc tacgccgtca ttagcgctat caaagggcac
    12121 cggcgtaacg ccacgataga gagcagtcag gttcagcgta cgttcatggc gcgacatggc
    12181 gaaaattggt agaccagagc tgatacggga ggtcatcagc gcggtacgac ccgattcggt
    12241 catggtaatg atcgccgtaa cgcccttcag gtggttagct gcgtacattg ccgacatggc
    12301 aatagcttct tccacattgt cgaactgaac gtccagacgg tgtttagaaa cgttgatgct
    12361 cgggattttt tccgcaccca ggcaaacgcg cgccatggct gcaacagttt ctgacggata
    12421 ctgcccagcg gcagtttctg cagacagcat cacagcgtca gtaccatcca gaacggcgtt
    12481 tgctacgtcc atgacttctg cacgcgtcgg catcgggtta gtaatcattg actccatcat
    12541 ctgggtcgcc gtgattaccg ctcggtttag ctgacgcgca cgacggatca acgctttctg
    12601 aatgccgacc agttccgggt cgccaatttc cacaccgagg tcgccacgtg caaccattac
    12661 cacgtcagag gcgaggatga tgtcatccat tgcatcctgg ctgcaaacgg cttccgcacg
    12721 ttcaaccttg gcaacaattt tcgcatcaca tcctgcatcg cgtgccaggc gacgggcata
    12781 gttcagatct tcgccacagc gtgggaagga gacagccagg taatctacgc caatcaacgc
    12841 cgcagtctta atgtctgctt tgtctttttc ggtcagcgct tcagccgaca aaccgccgcc
    12901 aagtttgttg atacctttat tgttggagag gggaccaccg acggtgactt cggtgaacac
    12961 tttcatgccc tgaacttcca gtacttttaa ctggacgcga ccatcgtcca gcagcaggat
    13021 gtcaccaggc acgacgtcag taggcaggcc tttgtagtcg ataccgactt tttctttgtc
    13081 gccttcacct ttacccaggt tggcgtcgag caggaattta tccccaatat tgaggaaaac
    13141 tttgccttct ttaaaggtgg atacacggat tttgggcccc tggaggtcac ccagaatagc
    13201 cacatgacgc cccagttttg cggcaatctc acgaacttta tccgcgcgca ttttgtgatc
    13261 ttcaggcgag ccgtgagaaa agttcatacg tacaacgttg gcacccgccg cgataacttt
    13321 ttcaagatta ttatcgcgat ctgttgctgg gcctaacgtg gtaacgattt ttgttctgcg
    13381 aagccttttg gacatgtaat actccgttga ctgaaacaac caggtgttgc ttgaacatga
    13441 aatccgaatg aaataacgcc gcgatggcga taaattaatc tcattcggat gtcgtataag
    13501 attaggacag tgacagtcgc ttttagcgat cgtcacttaa attaagtaac tgcttatcaa
    13561 aacgcgattc cttcagcgct tccttgaccc gcttcaagtt atctctgaat tttgcaccgc
    13621 gtcgcaaagt aaatcctgtc gccagcacat ctatcacggt cagctgtgca agtcgagaaa
    13681 ccatgggcat ataaatgtca gtatcttccg gtacgtcgag ggtaattgcc agcgttgctt
    13741 cccgggcgag cggggtaccc gcagaggtga gggcaatcac catggcgtcg ttttcgcgtg
    13801 ccagctgcgc cagctcgacc agattttttg ttcttcctgt gtgagaaatc agcaccacca
    13861 cgtctccgtc gctacaattc atacaactca tgcgttgcag cacgatatca tcggagtaca
    13921 ccaccggaac attaaaacga aagaacttat tcatcgcatc gtgggcaacg gcggcagaag
    13981 agcctaatcc gaaaaaggcg attttttttg cctgagtgag caagtcgacg gcgcggttga
    14041 tggcagattt atccagtgaa tgacggacat gatcgagcgt tgccattgcg gactcaaata
    14101 ttttccccgt gtatgattca acgctgtcat cttcattgac gttgcgatta acatagggag
    14161 tgccattcgc cagactttgt gccagatgaa gtttaaaatc aggaaaaccg cgcgtgtcca
    14221 tgctgcgaca gaaacgattc accgtcggtt cgctaacatt ggcttccagc gccagagcag
    14281 caatactcga atggatcgcg ttatcgggcg aagccagaat gacctcggca actttgcgct
    14341 ctgatttgct caaatgttcc agctgagact ggattttttc cagcatattc atgatgtaaa
    14401 gagactcacg ggtaatgacg atttccgcac tgaaagaaat cgaaatgcag ttttgtcaga
    14461 tattacgcct gtgtgccgtg ttaatgacaa aagcagataa aaaagttgtt atttttttca
    14521 taacatgatc agtgtcagat ttttaccgaa tggaaaacga tgattttttt atcagttttg
    14581 ccgcgctttg cgcgcttttc ccgtaatcgc acgggtggat aagcgtttac agttttcgca
    14641 agctcgtaaa agcagtacag tgcaccgtaa gaaaattaca agtataccct ggcttaagta
    14701 ccgggttagt taacttaagg agaatgacat ggcggtaacg caaacagccc aggcctgtga
    14761 cctggtcatt ttcggcgcga aaggcgacct tgcgcgtcgt aaattgctgc cttccctgta
    14821 tcaactggaa aaagtcggtc agctcaaccc ggacacccgg attatcggcg tagggcgtgc
    14881 tgactgggat aaagcggcat ataccaaagt tgtccgcgag gcgctcgaaa ctttcatgaa
    14941 agaaaccatt gatgaaggtt tatgggacac cctgagtgca cgtctggatt tttgtaatct
    15001 cgatgtcaat gacactgctg cattcaaccg tctcggcgcg atgctggatc aaaaaaatcg
    15061 tatcactatt aactactttg ccatgccgcc cagcactttt ggcgcaattt gcaaagggct
    15121 tggcgaggca aaactgaatg ctaaaccggc acgcgtagtc atggagaaac cgctggggac
    15181 gtcgctggcg acctcgcagg aaatcaatga tcaggttggc gaatacttcg aggagtgcca
    15241 ggtttaccgt atcgaccact atcttggtaa agaaacggtg ctgaacctgt tggcgctgcg
    15301 ttttgctaac tccctgtttg tgaataactg ggacaatcgc accattgatc atgttgagat
    15361 taccgtggca gaagaagtgg ggatcgaagg gcgctggggc tattttgata aagccggtca
    15421 gatgcgtgac atgattcaga accacctgct acaaattctc tgcatgattg cgatgtctcc
    15481 gccatctgac ctgagcgcag acagcatccg cgatgaaaaa gtgaaagtac tgaagtccct
    15541 gcgccgcatc gaccgctcca acgtacgcga aaaaaccgta cgcgggcaat atactgcggg
    15601 cttcgcccag ggcaaaaaag tgccgggata tctggaagaa gagggcgcga acaaaagcag
    15661 caatacagaa accttcgtgg cgatccgcgt cgacattgat aactggcgct gggccggtgt
    15721 gccattctac ctgcgtactg gtaaacgtct gccgaccaaa tgttctgaag tcgtggtcta
    15781 tttcaaaaca cctgaactga atctgtttaa agagtcgtgg caggatctgc cgcagaataa
    15841 actgactatc cgtctgcaac ctgatgaagg cgtggatatc caggtactga ataaagttcc
    15901 tggccttgac cacaaacata acctgcaaat caccaagctg gatctgagct attcagaaac
    15961 ctttaatcag acgcatctgg cggatgccta tgaacgtttg ctgctggaaa ccatgcgtgg
    16021 tattcaggca ctgtttgtac gtcgcgacga agtggaagaa gcctggaaat gggtagactc
    16081 cattactgag gcgtgggcga tggacaatga tgcgccgaaa ccgtatcagg ccggaacctg
    16141 gggacccgtt gcctcggtgg cgatgattac ccgtgatggt cgttcctgga atgagtttga
    16201 gtaatatcag cgcttatcct ttatgggtat tttaccggta acatgatctt gcacagattg
    16261 tagaacaatt tttacacttt caggcctcgt gcggattcac ccacgaggct ttttttatta
    16321 cactgactga aacgtttttg ccctatgagc tccggttaca ggcgtttcac tcataaatcc
    16381 tctgaatgaa acgcgttgtg aatcatcctg ctctgacaac tcaatttcag gagcctttat
    16441 gaatccacaa ttgttacgcg taacaaatcg aatcattgaa cgttcgcgcg agactcgctc
    16501 tgcttatctc gcccggatag aacaagcgaa aacgtcgacc gttcatcgtt cgcagttggc
    16561 atgcggtaac ctggcacacg gtttcgctgc ctgccagcca gaaaacaaag cctctttgaa
    16621 aagcatgttg cgtaacaata tcgccatcat cacctcctat aacgacatgc tctccgcgca
    16681 ccagccttat gaacactatc cagaaatcat tcgtaaagcc ctgcatgaag cgaatgcggt
    16741 tggtcaggtt gcgggcggtg ttccggcgat gtgtgatggt gtcacccagg ggcaggatgg
    16801 aatggaattg tcgctgctaa gccgcgaagt gatagcgatg tctgcggcgg tggggctgtc
    16861 ccataacatg tttgatggtg ctctgttcct cggtgtgtgc gacaagattg tcccgggtct
    16921 gacgatggca gccctgtcgt ttggtcattt gcctgcggtg tttgtgccgt ctggaccgat
    16981 ggcaagcggt ttgccaaata aagaaaaagt gcgtattcgc cagctttatg ccgaaggtaa
    17041 agtggaccgc atggccttac tggagtcaga agccgcgtct taccatgcgc cgggaacatg
    17101 tactttctac ggtactgcca acaccaacca gatggtggtg gaatttatgg ggatgcagtt
    17161 gccaggctct tcttttgttc atccggattc tccgctgcgc gatgctttga ccgctgccgc
    17221 tgcgcgtcag gttacacgca tgaccggtaa tggtaatgaa tggatgccga tcggtaagat
    17281 gatcgatgag aaagtggtgg tgaacggtat cgttgcactg ctggcgaccg gtggttccac
    17341 taaccacacc atgcacctgg tggcgatggc gcgcgcggcc ggtattcaga ttaactggga
    17401 tgacttctct gacctttctg atgttgtacc gctgatggca cgtctctacc cgaacggtcc
    17461 ggccgatatt aaccacttcc aggcggcagg tggcgtaccg gttctggtgc gtgaactgct
    17521 caaagcaggc ctgctgcatg aagatgtcaa tacggtggca ggctttggtc tgtctcgtta
    17581 tacccttgaa ccatggctga ataatggtga actggactgg cgggaagggg cggaaaaatc
    17641 actcgacagc aatgtgatcg cttccttcga acaacctttc tctcatcatg gtgggacaaa
    17701 agtgttaagc ggtaacctgg gccgtgcggt tatgaaaacc tctgccgtgc cggttgagaa
    17761 ccaggtgatt gaagcgccag cggttgtttt tgaaagccag catgacgtta tgccggcctt
    17821 tgaagcgggt ttgctggacc gcgattgtgt cgttgttgtc cgtcatcagg ggccaaaagc
    17881 gaacggaatg ccagaattac ataaactcat gccgccactt ggtgtattat tggaccggtg
    17941 tttcaaaatt gcgttagtta ccgatggacg actctccggc gcttcaggta aagtgccgtc
    18001 agctatccac gtaacaccag aagcctacga tggcgggctg ctggcaaaag tgcgcgacgg
    18061 ggacatcatt cgtgtgaatg gacagacagg cgaactgaca ctgctggtag acgaagcgga
    18121 actggctgct cgcgaaccgc acattcctga cctgagcgcg tcacgcgtgg gaacaggacg
    18181 tgaattattc agcgccttgc gtgaaaaact gtccggtgcc gaacagggcg caacctgtat
    18241 cactttttaa gacgacaaat ttgtaatcag gcgagagaaa actctgatga aaaactggaa
    18301 aacaagtgca gaatcaatcc tgaccaccgg cccggttgta ccggttatcg tggtaaaaaa
    18361 actggaacac gcggtgccga tggcaaaagc gttggttgct ggtggggtgc gcgttctgga
    18421 agtgactctg cgtaccgagt gtgcagttga cgctatccgt gctatcgcca aagaagtgcc
    18481 tgaagcgatt gtgggtgccg gtacggtgct gaatccacag cagctggcag aagtcactga
    18541 agcgggtgca cagttcgcaa ttagcccggg tctgaccgag ccgctgctga aagctgctac
    18601 cgaagggact attcctctga ttccggggat cagcactgtt tccgaactga tgctgggtat
    18661 ggactacggt ttgaaagagt tcaaattctt cccggctgaa gctaacggcg gcgtgaaagc
    18721 cctgcaggcg atcgcgggtc cgttctccca ggtccgtttc tgcccgacgg gtggtatttc
    18781 tccggctaac taccgtgact acctggcact gaaaagcgtg ctgtgcatcg gtggttcctg
    18841 gctggttccg gcagatgcgc tggaagcggg cgattacgac cgcattacta agctggcgcg
    18901 tgaagctgta gaaggcgcta agctgtaaaa gtcaaaatgc ccgatctagg atcgggcatt
    18961 tttgtagcgt ttttttgccg ggtttaaccc tgtactttta cctgtccggc ggcgtgcttc
    19021 gcgcgttcaa tggcgtcaac aacactctct gaagtagcca gtaccacccc cagatgacgg
    19081 ctgccatcaa tttccggctt accaaataaa cgaatctgca aatctgcgcc tacggcattc
    19141 tgcacattat caaacgtgac attctgactg gtcagttgtg gcagaataac ggcagaagct
    19201 gcaggaccat actgacggat cccgccaaca ggaagtccga ggaaggcacg tacatgcagg
    19261 gcaaactctg agagatcttg agaaattaac gtcaccatcc cggtatcatg tggacgaggg
    19321 gagacctcac tgaaaatcac ctcatcacca cagacaaaca gctcgacacc aaacagacca
    19381 taaccgccca gtgccagcac cactttacgg gcaatctcct gcgcacgttc aagggcaagc
    19441 gggctcattt gctgtggttg ccaggattca cggtagtcgc catcttcctg gcgatgacct
    19501 actggtgcac agaaatggac gccatccacc gcgctgacgg ttagcagggt aatttcgaag
    19561 tcaaacttaa cgacgccttc aacaattacg cggcccgctc cggcgcgacc gccttgctga
    19621 gcgtattccc atgcctgagc aagttgctct gcagagcgaa taaacgtctg ccccttgccg
    19681 gaagagctca tcaccggttt tacaatgcag ggatagccaa tggcggcaac cgcctcgcgg
    19741 aaaaggcttt tgctatcggc aaaacgataa gtggaagtgg gcagctgcag ctcttctgcc
    19801 gccaggcgac ggataccctc gcgattcatc gttaatttcg ttgcgcgagc gcaggggaca
    19861 acattcagtc cttcctcttc aagttggatc agcatatcgg tggcaatagc ttcgatctcc
    19921 ggcacgatat aatgtggttt ttccagttca accacacggc gtaatgcatc accatcaagc
    19981 atattaatga catgggagcg atgcgcgaca tgcatggctg gtgcgtcggc atagcgatcg
    20041 acggcaatca cctctacgcc gagacgctga cactcgattg ccacttcttt acccagttca
    20101 ccggagccta ataacatcac gcgagttgct gccggacgca gcgcagtgcc taataacgtc
    20161 ataacgaata tcccctgatt aattccgcgc gcagtataaa cgaaaacgtt tgcgtgtgtc
    20221 tttattgctg atgttgattt ctcaaccgaa aagaaatata ctgtataaaa tctcagttat
    20281 tatgagaggc tgcaaatggc ggttgaagtt aaatacgtag tcattcgtga gggcgaagag
    20341 aaaatgtcgt ttaccagcaa aaaggaagcc gatgcttatg acaaaatgct tgatacggcg
    20401 gatctgcttg acacctagct gacaaattct ccagtgcaaa tggaagacga gcaacgtgaa
    20461 gccctttcgc tatggctggc agaacaaaaa gaggtgctga gcaccattct gaaaaccggc
    20521 aaattaccgt ccccacaggt cgtcggtgct gaatccgaag aggaagatgc ctcacacgcg
    20581 gcctgaagca acttgcgctc ctgacaattt gtaccatgct ttctcctgaa tcattgtgct
    20641 atggagaaaa acatgaaaaa aagaggggcg tttttagggc ggttgttggt ttctgcctgc
    20701 gcatcagttt tcgctgccaa taatgaaacc agcaagtcgg tcactttccc aaagtgtgaa
    20761 ggtctggatg ctgccggaat tgccgcgagc gtaaaacgtg attatcaaca aaatcgcgtg
    20821 gcgcgctggg cagatgatca aaaaattgtc ggtcaggccg atcccgtggc ctgggtcagt
    20881 ttgcaggaca ttcagggtaa agatgataaa tggtcagtac cgctaaccgt gcgtggtaaa
    20941 agtgccgata ttcattacca ggtctgcgtg gactgcaaag cgggaatggc ggaatatcag
    21001 cggcgttaat ggcttctgat aattcgattg accgttggcg gatgcggcgc gagtgccttg
    21061 tccgtcccag aacgcaccac aatcgtaggc ctgagaagac gcaacagcgt cgcatcgggc
    21121 aatcagcatc aaacaatgcc actcaaaacg acaaactact ttcacctaaa atacccttat
    21181 cacctgatat acagtaaata ccttacctgg cgcgaatttc tttgaataac ggcctaccgc
    21241 cccgcttatt tgcgcatctt ttgtctcgat atacattttc tgacatcctc atccgctatg
    21301 ctacaggcac taaaaatggt tattgaggag caaatatggc taactggtta aatcaactgc
    21361 aatccctgct tgggcaaagc agttcttcta cctcctcgtc tgcggatcag ggattgggca
    21421 aactgttagt gtcaggcgca ttaggcggac tggctgggct gctggtcgca aataaatcag
    21481 cacgtaaact tcttacgaaa tatggcacca acgcgttact ggttggcggc ggagcggtag
    21541 cgggtacggt gctgtggaat aaatacaaag ataaaattcg cgcggcgcat caggacgaac
    21601 cgcagtttgg cgcgcaaagt acgccgctgg atgagcgtac ggaacgtttg atccttgcgc
    21661 tggtctttgc cgctaaaagt gatggtcata ttgatgccaa tgaacgtgcg gcaatcgacc
    21721 agcaattgcg tgaagccggc gtggaagagc aggggcgtgt actcattgag caggcaatcg
    21781 aacaaccgct ggatccacaa cgcctggcta ccggggtccg caatgaagaa gaggcgctgg
    21841 aaatctattt cctgagctgc gcggctattg atattgacca ttttatggaa cgcagttacc
    21901 tgaatgcact gggtgacgcg ctgaaaatcc cccaggatgt tcgtgaaggc attgaacgcg
    21961 atctcgaaca gcaaaaacgc actttggcgg aataattccg ctctctggat agcctgaagt
    22021 tgactatttc gggctatcca gaaccgcaca gccatttgaa caggctgatt ttctctgtct
    22081 gcataacatt cgatatccgc gccattacct gtcatcatct aagcaatgac gaccctgttt
    22141 cgcttgcatc cccggtgagt tttgccaccc ttataagatg tttccaacca gaaagaacaa
    22201 taacatgcta ccaaaagccg cccgcattcc ccacgccatg acgcttcatg gcgatacgcg
    22261 catcgataat tactactggc tgcgggacga tacgcgttct cagccaaaag tcctggacta
    22321 cctgcaacaa gaaaatagtt acggtcatcg ggtgatggcc tcacaacaag ccttgcagga
    22381 tcgcatctta aaggaaatca tcgaccgcat tccgcaaaga gaagtttctg cgccctacat
    22441 aaaaaatggc taccgctatc ggcatattta tgaaccaggc tgtgaatatg ctatctacca
    22501 gcgtcaatcg gcattcagcg aagagtggga tgagtgggaa acattgctcg atgccaacaa
    22561 gcgcgcagct catagtgagt tttattcgat gggcggaatg gcgattacgc ccgataacac
    22621 cattatggcg ctggcagaag attttctttc ccgacgccag tacggcattc gttttcgtaa
    22681 tctggaaaca ggtaactggt acccggaact gctggataac gttgaaccca gctttgtctg
    22741 ggcaaatgac tcctggactt tctactatgt tcgcaagcat ccggtgacgc tgctgcctta
    22801 tcaggtctgg cgtcacgcca tcggtacgcc agcatcgcaa gatacactga tctacgaaga
    22861 aaaagacgat acctattatg tcagcctgca taaaacgacg tcgaagcact atgtcgtcat
    22921 tcatctggcc agcgccacca ccagtgaagt tcgcctgctg gacgcggaaa tggccgatgc
    22981 cgagccgttt gtttttctgc cgcgccgcaa agatcacgaa tacagccttg atcactacca
    23041 gcatcgtttt tatctgcgtt ccaatcgcca cggcaaaaac tttggcttat accgtacccg
    23101 tatgcgtgat gagcaacagt gggaagagtt aattccgcca cgcgaaaaca tcatgctgga
    23161 agggtttacg ctgtttaccg actggctggt ggttgaagag cgtcagcgcg ggttaaccag
    23221 tttgcgccaa attaaccgca agacccggga agtcattggc attgcctttg atgatccggc
    23281 ctatgtgacc tggattgcct acaatccaga acctgaaacc gcgcgattgc gttatggtta
    23341 ttcttccatg accacaccag acactttgtt tgaactggat atggataccg gtgagcgtcg
    23401 tgtattaaaa caaacagaag tgcctggttt tgatgcggcg aattaccgca gtgaacacct
    23461 gtggatagtc gcccgtgatg gcgtcgaagt tccggtttcg ctggtctatc atcgcaaaca
    23521 ttttcgcaaa ggacacaacc cgttgctggt gtatggctat ggttcttacg gcgcaagtat
    23581 tgatgccgat ttcagtttta gccgcttgag tttgttagat cgtggctttg tctacgccat
    23641 tgtccatgtt cgcggcggtg gtgagctggg gcaacaatgg tacgaagacg gtaaatttct
    23701 gaagaagaaa aatacgttta atgattatct tgatgcctgc gatgcattgt taaaactggg
    23761 ctatggctct ccttcgctct gttatgcgat gggcggaagt gcggggggca tgttgatggg
    23821 cgttgcaatt aatcaacgcc cggaattatt ccacggcgtt atcgcccagg taccgtttgt
    23881 tgatgttgta acaacgatgc ttgatgaatc aattcctctt accactggtg agtttgaaga
    23941 gtggggaaat ccgcaggatc cgcaatatta cgagtacatg aaaagctaca gcccgtatga
    24001 caacgtcacc gcacaggctt atccgcattt actggtaacg accggtttac acgattctca
    24061 ggtgcaatat tgggaaccgg caaaatgggt cgctaaattg cgcgagctga aaaccgatga
    24121 ccatctttta ttgctctgta ccgacatgga ctcaggccat ggcggtaaat ctggccgctt
    24181 taaatcgtac gaaggcgtag cgatggaata tgcttttctg gtcgcgctgg cgcagggaac
    24241 attacccgct acgcctgcgg attaagtatt ttccagataa tgtttcagtg ttaaacgcag
    24301 ctccgggctc atgctgtcca ggttattaaa taaccagcgc agatagcccg gatagcgttc
    24361 ggcaacgtcg gaaaccgctt tgccacggta tttgccaaag gtgaaggttg tcatcaacga
    24421 cggacgtccg gtgatatcgg ccatctgttc tgccgtccag ccggaggtgt tcataatatc
    24481 gataagcaac gcggcggtga tataacagtc atacaacgcg cggtgatgat gcaggcccag
    24541 cggtgtctgt acattgagct tgcgtgtttt atataacgcc atattgctgt acttgagccc
    24601 tggccacaaa cgacgggcca gtttcatagt gcaaatccac tcaccgggca tctcaggcag
    24661 tacgcggcgg tcaaagctgg cgttgtgcgc gacataccat tcactaccgt aatagtgtgg
    24721 gatcacatct tcaatccacg gtttatcggc gaccatggct tcggtgatgc gatgaatcgc
    24781 catcgcttgt ggactaatag gacgatcggg gcgcaccagg aggctcatgg ggttgacgat
    24841 ttttccgtca atgacatcaa cagaggcaat ctcaacgatc cctccctgca aaccgcaggt
    24901 ttctgtatcg ataatgcgca acatggcatg atccaggccg aaaaagccta gcgtaatgga
    24961 atgatatcgc cttgccaacc ctgtgatgaa cgctgaccga ctaacaataa tgaaccacga
    25021 tcggcgcgaa cgatgagacg accatattca tcccatagtg cactgttacc acgggcattt
    25081 gccatcagca cggcaatcga gtactgatgt gaaaaaaatt gtaagcgacg ggccgacgcc
    25141 agcaggtcgg gttctcccag acattgccct gtcgtaaaca gtgaacaggt cgggtccata
    25201 tccatacctt gcggttgttc atcgaccacc gtgatggttc tggaacgacg gcccagacat
    25261 gcaccatgac tctgatggta gttcccaggc gtttttcgcc acggtgcgaa caccgcaatt
    25321 ccacgaataa agcgatcgtt atattcaacg ggaaggccgg caatgatggt cattcgctag
    25381 gtcgttgcgg cataacaaag cgggtctaac agtgaaagat cgggtggggc aggaagggca
    25441 cgtcgcgaat aatcacaccc cagtaaagaa agcgatggaa aaactaacag ctgacattgt
    25501 tgcctggcgg cggccctcac aaactccaga tgatgagcta cctgttcggt gagtgaggtt
    25561 ttacgaggct catattgcgc tgctgcaact ttccagaacg acataatgac ttccttttca
    25621 aaatgactgc aaattatggg ggcggtttag aaaatactca taatttagca ggcgttatgt
    25681 aagaaagtgt aactctgtat aagttttatt taagtttggg ctcgtaaggt aaacgtgaca
    25741 gattgaccga agccaaacgg tgggcaataa gccgctcgcg aaaccagctg cgcaaatgtt
    25801 ctggctgttc acgttcaacc gcttcagcga tcaccggcat attgtagcgt tctttaaaag
    25861 ccaccccggc cgccgccaaa tcgacattca ctttatccat ttctgtttga tccagtttag
    25921 ccagattctt cagcattctt aatctcctca tcattcgcgg gcaaaagtta ctaagagtga
    25981 gcctggatgg caagccggaa ttgatgatcc tcgactcaga cattagacaa ggttatgctc
    26041 tgtaaaaaag ttattacaaa aaggaataga taatatggct tcaactgcac gctcccttcg
    26101 ctacgcgctg gcaatcctga ccacttcgtt ggtgacacct tccgtctggg cacatgcgca
    26161 tttaacgcat cagtatcctg cggc
//
LOCUS       DAZPAB010000037        25619 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZPAB010000037 DAZPAB010000000
VERSION     DAZPAB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.037"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(32..367)
                     /locus_tag="ACHUPG_001398"
     CDS             complement(32..367)
                     /locus_tag="ACHUPG_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR0861073.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            769..2997
                     /gene="crfC"
                     /locus_tag="ACHUPG_001399"
     CDS             769..2997
                     /gene="crfC"
                     /locus_tag="ACHUPG_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR0861074.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            2994..3872
                     /locus_tag="ACHUPG_001400"
     CDS             2994..3872
                     /locus_tag="ACHUPG_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFR0861075.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            4136..5638
                     /gene="proP"
                     /locus_tag="ACHUPG_001401"
     CDS             4136..5638
                     /gene="proP"
                     /locus_tag="ACHUPG_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR0861076.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            5750..5839
                     /gene="pmrR"
                     /locus_tag="ACHUPG_001402"
     CDS             5750..5839
                     /gene="pmrR"
                     /locus_tag="ACHUPG_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR0861077.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5815..6915)
                     /gene="pmrB"
                     /locus_tag="ACHUPG_001403"
     CDS             complement(5815..6915)
                     /gene="pmrB"
                     /locus_tag="ACHUPG_001403"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR0861078.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(6916..7584)
                     /gene="basR"
                     /locus_tag="ACHUPG_001404"
     CDS             complement(6916..7584)
                     /gene="basR"
                     /locus_tag="ACHUPG_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR0861079.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(7581..9224)
                     /gene="eptA"
                     /locus_tag="ACHUPG_001405"
     CDS             complement(7581..9224)
                     /gene="eptA"
                     /locus_tag="ACHUPG_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR0861080.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(9328..10665)
                     /gene="adiC"
                     /locus_tag="ACHUPG_001406"
     CDS             complement(9328..10665)
                     /gene="adiC"
                     /locus_tag="ACHUPG_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR0861081.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10802..11563)
                     /gene="adiY"
                     /locus_tag="ACHUPG_001407"
     CDS             complement(10802..11563)
                     /gene="adiY"
                     /locus_tag="ACHUPG_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR0861082.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11888..14155)
                     /gene="adiA"
                     /locus_tag="ACHUPG_001408"
     CDS             complement(11888..14155)
                     /gene="adiA"
                     /locus_tag="ACHUPG_001408"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR0861083.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(14354..15262)
                     /gene="melR"
                     /locus_tag="ACHUPG_001409"
     CDS             complement(14354..15262)
                     /gene="melR"
                     /locus_tag="ACHUPG_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR0861084.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            15545..16900
                     /gene="melA"
                     /locus_tag="ACHUPG_001410"
     CDS             15545..16900
                     /gene="melA"
                     /locus_tag="ACHUPG_001410"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR0861085.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            17003..18424
                     /gene="melB"
                     /locus_tag="ACHUPG_001411"
     CDS             17003..18424
                     /gene="melB"
                     /locus_tag="ACHUPG_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFR0861086.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(18563..19192)
                     /locus_tag="ACHUPG_001412"
     CDS             complement(18563..19192)
                     /locus_tag="ACHUPG_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR0861087.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(19315..20961)
                     /gene="fumB"
                     /locus_tag="ACHUPG_001413"
     CDS             complement(19315..20961)
                     /gene="fumB"
                     /locus_tag="ACHUPG_001413"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR0861088.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(21039..22379)
                     /gene="dcuB"
                     /locus_tag="ACHUPG_001414"
     CDS             complement(21039..22379)
                     /gene="dcuB"
                     /locus_tag="ACHUPG_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR0861089.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(22950..23669)
                     /gene="dcuR"
                     /locus_tag="ACHUPG_001415"
     CDS             complement(22950..23669)
                     /gene="dcuR"
                     /locus_tag="ACHUPG_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR0861090.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(23666..25297)
                     /locus_tag="ACHUPG_001416"
     CDS             complement(23666..25297)
                     /locus_tag="ACHUPG_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR0861091.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            25478..>25619
                     /gene="yjdI"
                     /locus_tag="ACHUPG_001417"
     CDS             25478..>25619
                     /gene="yjdI"
                     /locus_tag="ACHUPG_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR0861092.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
BASE COUNT         6190 a         6208 c         6605 g         6616 t
ORIGIN      
        1 cgccttatcc ggcttaccga tcacaataca atcagttctt tttcacaaac tcagatttca
       61 gtttcatcgg accaaaaccg tcgattttgc aatcgatgtt atggtcgcct tcaaccaggc
      121 ggatgttttt cactttggtg ccaattttca gcatcgaaga gctacctttc accttcagat
      181 ctttaatgat ggtaacgctg tcgccgtcag ccagcagatt gccgttagca tctttaacga
      241 tcagctcgtc gctttcctgt gcaggttctg cgtcgttcca ttcgtaggca cattccgggc
      301 agatgtacat gccgttatct tcgtaagtgt attcggagtt gcattttggg cagtgtggta
      361 atgacatgtg gatttcctca aaagtcagca gggcaaaagc gccaaaaaac ggcagattgc
      421 cgaaaaaggc cgcaattata cacaaaatcc ctgagtttgt cgggggtact gctgatttta
      481 gacgacgatc gttttcattt gaaaggaaac gcaaaaagaa aataatcttt ttgcctctat
      541 ttattattta agataatgtt aatgctctac ttgttctttt tttattcaca tgatattttt
      601 atctttctga cgttttaaac gcatcagaag acgttattta ttctgataaa aaaacaacac
      661 actaaatata aaattatttc ttgcagtaat tataaaagcc gaaaacagaa ctcaaaaaca
      721 atctggctaa ataaaagtaa caaaatttgc tttaaggaag aattttctat gtacacacag
      781 accctgtatg agttaagtca ggaggctgaa cgcctgttac agctttctcg ccaacagttg
      841 cagttactgg aaaaaatgcc tctctctgta cccggagacg acgcgccaca actggcttta
      901 ccctggagtc agcctaatat cgccgaacgt cacgcgatgc tgaataatga gttgtgtaaa
      961 atttcccgac tggaaatggt gctggcaatt gtcggtacca tgaaagcagg gaaatcaacc
     1021 accattaatg ccattgttgg tacggaggtt ctgcctaatc gtaatcgccc aatgactgcg
     1081 ctgccgacgc ttattcgcca tacgcccggg caaaaggaac cggtactgca tttttcacat
     1141 gtcgcgccaa tcgattgttt aattcaacaa ttacaacagc gcctgcgtga ttgcgatatt
     1201 aagcatctga ccgatgtgct ggaaatagat aaagatatgc gtgcgcttat gcagcggatc
     1261 gaaaatggcg tcgctttcga aaaatattat ctgggtgccc agcctatttt tcattgtctg
     1321 aaaagtttga atgatttagt gcgactggcg aaggcgctgg acgtcgattt tcctttttct
     1381 gcttacgccg ccattgagca tattcccgtg attgaagtgg agtttgtcca tctggcgggg
     1441 ctggaaagtt atcccggtca gttgacgtta ctggataccc ccgggccaaa tgaagccggg
     1501 caaccgcatc tgcaaaaaat gcttaaccag cagctggcac gcgcctcggc ggtactggcg
     1561 gtgctggatt atacgcaact gaaatcgatc tccgatgaag aggtccgtga ggcgattttg
     1621 gcggtggggc aatcggtgcc gctgtatgtg ctggtcaata agttcgatca acaggatcgt
     1681 aacagtgacg acgccgacca ggtgcgggca ctgatttccg ggacgctgat gaaaggctgt
     1741 attacgccac agcagatatt cccggtgtcg tcgatgtggg gctacctggc gaatcgggcg
     1801 cgctatgagt tagccaacaa cggtaagtta ccagcgccag agcaacaacg ctgggtggaa
     1861 gattttgccc atgccgcgct cggcaggcgc tggcgtcatg ccgatctggc ggacctcgaa
     1921 catattcgtc atgctgccga tcagttgtgg gaagattcgc tgttcgccca gccaattcag
     1981 gcgttgcttc atgccgctta cgctaacgcc tcattgtatg ctctgcgatc tgctgcgcat
     2041 aaactgttga attacgcgca gcaggcgcgg gaatacctgg attttcgtgc gcacgggtta
     2101 aacgtcgctt gtgaacaatt gcggcaaaat atccatcagg tcgaagaaag tttgcagcta
     2161 ttgcaactca atcaggcgca ggtgagcggc gagattaaac atgaaatcga gctggccctg
     2221 acctccgcca accactttct gcgtcaacag caagatgcgg tgaatgcgca gttagccgcc
     2281 ttgtttcagg atgattcgga gccgttaagc gagattcgta cccgctgtga gacactgtta
     2341 cagacggcgc agaacaccat cagtcgcgac tttacgctgc gttttgccga gatggaatcc
     2401 accctttgcc gggtgttaac cgatgttatt cgccccattg agcaacaagt caaaatggaa
     2461 ttgagcgagt cagggtttcg tcctgggttt cattttcctg tttttcacgg cgtagttccc
     2521 cacttcaaca ctcgccagct gttcagtgaa gtcatttcgc gccaggaagc aacggacgag
     2581 cagagcacgc gtttaggcgt tgtgcgtgag actttttcgc gctggttgaa tcagcccgac
     2641 tggggacggg gaaatgagaa atccccgaca gaaacggttg attacagtgt gttgcaacga
     2701 gcattaagcg cagaagtcga tctctattgc caacaaatgg ctaaagttct ggcagagcag
     2761 gtcgatgaat ctgttacggc aggcatgaat acttttttcg ctgagttcgc ttcatgtttg
     2821 acagaattac agacgcgttt acgcgaaagt ctggctctgc gtcaacaaaa tgaatcggtg
     2881 gtcaggctga tgcagcagca attgcagcag actgtgatga ctcacagctg gatttacacc
     2941 gacgctcagc tgttacgcga tgatattcaa acacttttca cggcagaacg atattgacca
     3001 agacgttact ggacggcccc ggtcgcgtgc tggagtcggt tcatccgcgc tttttagtgg
     3061 atctggcgca gggcgatgat gcccgccatc ctcaagccca tcagcagcag tttcgtgaac
     3121 gactgatgca ggaacttctt tcgcgtgtgc agcttcagac atggacgaac ggcggcatgt
     3181 taaatgcgcc gcttagcctg cgactgacaa tggtggaaaa actggcgtcg atgctggatc
     3241 ccggtcatct ggcgctgacg cagatcgcgc agcatctggc gctgctgcaa aaaatggatc
     3301 accgccagca ctctgctttc ccggagctcc cccagcaaat tgccgccttg tatgagtggt
     3361 tttcagcccg ttgtcgctgg aaggaaaagg cgttaacgca acgaggccta ctggtgcagg
     3421 caggtgatca gagcgagcaa atttttaccc gctggcgtgc tggggcgtat aacgcctggt
     3481 cgttgcctgg gcgctgtttt atcgttctgg aggagttgcg ctggggtgca tttggcgatg
     3541 cctgccgtct gggaagcccg caagcggtgg tgttgttgct gggtgatttg cgcgagaaag
     3601 cgacacaaca tctggcagag agtatcaatg cggcaccgac tacgcgtcac tattaccatc
     3661 agtggtttgc ctcttcgacc gttccgacgg gcggggatca tgctgatttt ttaagttggc
     3721 tgggaaagtg gaccacggca gataaacaac ccgtttgctg gtcagtgacc caacgctggc
     3781 aaactgtcgc gctggggatg ccacgactct gttcagcgca gcgtttggtg ggggcaatgg
     3841 tcgaggaaat cttctctgta aatttggcgt aaataatcag ttacatcaat gagtcctaaa
     3901 cgaaatccat gtgtgaagtt gatcacaaat ttaaacactg gtagggtaaa aaggtcatta
     3961 actgcccaat tcaggcgtca actggtttga ttgtacattc cttaaccgga gggtgtaagc
     4021 aaacccgcta cgcttgttac agagattgca tcctgcaatt cccgctcccc ttttgcggcc
     4081 gtcgcgctga tttttctggc gtttgcggaa atgggccaac tctgcgagga aagctatgct
     4141 gaaaaggaaa aaagtaaaac cgattaccct tcgtgatgtc accattattg atgacggtaa
     4201 actgcgtaaa gccattaccg cagcatcact gggtaatgca atggaatggt ttgattttgg
     4261 tgtttatggt tttgttgctt acgcattagg taaagttttt ttcccggggg ctgaccccag
     4321 cgtgcagatg gttgctgcac ttgccacttt ctccgttccc tttctgattc gaccgcttgg
     4381 cggactcttc tttggtatgt tgggcgataa atatggtcgc cagaagatcc tcgctatcac
     4441 tattgtgatt atgtcgatca gtacgttctg tattggctta ataccgtcct acgacacgat
     4501 tggtatttgg gcaccgattc tgctgttgat ctgtaagatg gcacaaggtt tctcggtcgg
     4561 tggtgaatat accggggcgt cgatatttgt tgcggaatac tcccctgacc gtaaacgtgg
     4621 ctttatgggc agctggctgg acttcggttc tattgccggg tttgtgctgg gtgcgggcgt
     4681 ggtggtgtta atttcgacca ttgtcggcga agcgaacttc ctcgactggg gctggcgtat
     4741 tccgttcttt attgctctgc cgttagggat tatcgggctt tacctgcgcc atgcgctgga
     4801 agaaactccg gcgttccagc agcatgttga taaactggaa cagggcgacc gcgaaggttt
     4861 gcaggatggc ccgaaagtct cgtttaaaga gattgccact aaatactggc gcagcctgtt
     4921 gacatgtatt ggtctggtaa ttgccaccaa cgtgacttac tacatgttgc tgacctatat
     4981 gccgagttat ttgtcgcata acctgcatta ctccgaagac cacggggtgc tgattattat
     5041 cgccattatg atcggtatgc tgtttgtcca gccggtgatg ggcttgctga gtgaccgttt
     5101 tggccgtcgt ccgtttgtgc tacttggtag tgttgcactg tttgtgttgg cgatcccggc
     5161 gtttattctg attaacagta acgtcatcgg cctgattttt gccgggttac tgatgctggc
     5221 ggtgatcctt aactgcttta tgggcgttat ggcttctacc ttgccagcga tgttcccgac
     5281 gcatatccgt tacagcgcgc tggcggcggc atttaatatt tcggtgctgg ttgccggtct
     5341 gacgccaaca ctggcggcct ggctggtcga aagctcgcag aatctgatga tgcctgccta
     5401 ttacctgatg gtagtggcgg tgattggttt aatcaccggc gtaaccatga aagagacggc
     5461 aaatcgtccg ttgaaaggtg cgacaccggc ggcgtcagat atacaggaag cgaaggaaat
     5521 tctcgtcgag cattacgata atatcgagca gaaaatcgat aatattgacc acgagattgc
     5581 cgatttgcag gcgaaacgta cccgcctggt gcagcaacat ccgcgaattg atgaataagc
     5641 tgaaacggat ggcctgatgt gacgctgtct tatcaggcca attgaactct taaggttcac
     5701 ttaatctctg acgcgcatac tctcctccag gttaacggag gagagtgcaa tgaaaaaccg
     5761 tgtttatgaa agtttaacta ccgtgttcag cgtgctggtg gtcagcagct ttctttatat
     5821 ctggtttgcc acgttctgat ctttcttcag ccgtacccag gcccgcgtgc cggaagtctc
     5881 ttgccggttt tgcaggaaaa actgcccgtg atgcaactgt gtaatgcggc tgacaatact
     5941 taaccccaga ccaatcccgc cataacggct gtccatacgt acaaacgctt tactcaactc
     6001 cccgcattta ctctcatcaa tacctggtcc ttcatcttca actgccatga ccgctccgcc
     6061 atcttcttgc agcttaatca taatgttgct gccttgcggg ctgtaacgat gggcgttttc
     6121 taccaggttt cgcaataaca tccgcagcag ggttgcatca ccctgaacgg tgatgtcggc
     6181 ggcgctctct ggcaatagca gggtttgctg tcgctggtcg agcatggtac tgagttcgtc
     6241 atacgagggg agaatgacat cttccagcag ttttacatgt tgataattac cggaagaaaa
     6301 tgactgtccg gcacgcgcca gttgcagcag ctgggagacg ctctccatca tctgatcaag
     6361 ccgtgccact aacggtgcta catcaatgtg atgcgttttc gccagcagtt ccagatgcaa
     6421 acgcaccccc gccagtggcg ttcgcagttc gtgcgcgacg tcagcggtaa acaacctttc
     6481 gttatccagc gtgctggtca ggcgactgac cagatcgttc aacgccgaaa ccaccgcttc
     6541 gatttcgagg gtggcgctgt gaatggcaat gggcgttaag ttgtcggcgg tgcgcgcttc
     6601 cagctctttt tgcagctccg ccagcgggcg ggtgatgcgg cgtaccgcct gatagcagat
     6661 aaatagcgtc aggctgacca taaagacgcc ggggacaatc aggctggcga ccgcctcgcg
     6721 gatctcacgc atgatgtggc gatcgttgtt gcgattgtcg cgtagcgcct gctcaaacag
     6781 ctgaatctgc tcggtacttt catgccatag ccagaagacg ctgatcagct caaacaccaa
     6841 caaaatggcc ccgatggtca atatcagccg ttggcgcagc gatataggtc ggcgcagaaa
     6901 acgcatcaga ttcaattagt tttcctcatt cgcgaccagc atatagccaa agccgcgcac
     6961 ggtgcggata cgggctttgc ccactttgtc gcgcagattg tggatatgca cttccagggt
     7021 gttggtcgag ggttcattgt cccagttata gatgtcgttg tagagaattt cccgatgtac
     7081 cggactgcct gctttgagca ttaaccgtga cagcagagca tattctttgg gcgtcagaat
     7141 caactcttca ccgcccatcc atacctgacg gcgacccata ttcagcgtca gattgccaac
     7201 aatcagctca ctttcgccct gattattatg gcgtcgtagc agggcgcgga tacgggcgtg
     7261 taactcttcc agcgcaaaag gcttcaccag atagtcatcg gcaccgacat ccagcccggc
     7321 gattttgtcg gtcagcgtat cgcgagcggt gaggatcagt accggcaggg tgtatttttt
     7381 ctgccggata cgggcgagaa aatgcagtcc atcttcgtcg ggtaacccta aatccagtac
     7441 caccaggctg taatgaccgt cctcaaggct ttgttccgcc atccgcgcgg ttgtcacgcc
     7501 atcgcacgcg tagccttcgg tttgcgccgc cagaatcagt ccctgcaata acagcgtatc
     7561 gtcttcaaca atcagaattt tcatttactc actctcctgc aagtttgcag aatatcatcc
     7621 gcggcctggt aatacttcgt ctcaacgcca gttaatccca atagcgtgga gaataaattg
     7681 tcttgtgaat agtgttgcgt ttgcgcctgt ttttgcaggc agttctggtc aacctgatac
     7741 cgcttttgat aatcctccga cagccacagc agcattggca cctgtttttg gctatccggg
     7801 gcgatggcat aaggcagacc gtgcagatag atgccatttt cacctaacga ttcaccgtgg
     7861 tcagaaagat aaaccaggct ggtggtaaat ttatcctgat gttctttaag cagattaatc
     7921 gctttatcaa caatatagtc gacgtaaacc agcgtgttgt cgtaagtgtt caccagttgc
     7981 tctttggtac aggtctggat ctcattggtg tcgcaggttg gggtaaattt cctgaactgc
     8041 ggcggatagc ggttgtaata ggtcggaccg tggctgccga tggtgtgtaa gacaatcacg
     8101 ccatcaccct gcaggttatt gatgtactct tccagcccgt ggaacagcac ttcgtcatag
     8161 cattcgccgt tgatgcactg atcaggcaga ttcagcgcgg tgacgttctg gtgaggcacg
     8221 cggtcgcagg cacctttaca gccgccatcg ttgtcattcc acagcacgtt gatgcccgct
     8281 cgctgaatga tatccagcac gccttcctgg tgctgtgcca gctcttcttt gtagtgctca
     8341 cgcggcatat ccgagaacat gcacggtact gaaactgccg ttgccgtgcc gcaagatgcg
     8401 gtattaggga aatagaccac gttatctttc gccagccgcg ggttagtttc acgcgggtag
     8461 ccgttgaggg agaagttctc cgcccgcgag gtttcgccga caatcaggat ggtcaaattt
     8521 ttacgttttt cgttctgcat taacgggttg cggtgcgcgt cttcaccaat tcgcaccagc
     8581 ggcagatttg ccagtcgctg atgggagtac catgaccagc tggcaacaat gctgttagag
     8641 gggcttaagg atttcaccag ctctttgtta ttgcggaaca acgaggcgta gtctttataa
     8701 aacagtgcgg cgaccagcaa aatcagcagt acagaaacca gaatattggc tccacggaaa
     8761 agaacactgc gcagacgcga ggtggcaggt ttgattttta gccagcaggc aatcagctca
     8821 gcaagcacgc cgctgaatcc cagcgttaat aacatttgcg gtgtcatcag cgcataactt
     8881 tctgctggag tggtatcaat aatattggca atcatcgagc ggtcgatgac gatgccgtaa
     8941 gtcattatga aatattgtgc agccgcgcca atcagaataa acaggcaggc cagtggtcga
     9001 ttaagccata agaaagagct tagtgtcagg acaatattaa tcacgctgaa agcgacaacc
     9061 ggcatcgaca agaaaaccag tacgttatgc agcgaatcca gcggcagcgc ctgcaacacc
     9121 tgtttaaaaa aggcaatatt caggcagata gagatataaa aagcggccaa caatagccag
     9181 gcgagtaaat tcaaagaggg tctttttagt aggcgcttca acatcacggt gtttccatcg
     9241 aacaaagtgc gcatatgctc gcaaagcaaa attaagccaa ccttaagttc ttaaggttgg
     9301 cttttatgtt tgctggatta aggggaatta atctttgctt attggtgcat ctaagggata
     9361 cgggttttta tgtagccggt tgtaattcag cgcatacatg gcggtgatga ccatcagggt
     9421 gacaaatgac cacataacct ctttcgcccc ggaccccacc acggcccaaa tgcagtagag
     9481 gaaggcaatg gtagtaacag ccagatatgc cgggcgtgct ttaccaaagt gaccgtggcc
     9541 gagcagcagt aacgccgcac aggtgtaaag atatggcacc agtgtaaaga tgaccgagac
     9601 ggaagaaacc agaccgaact ctttggtcgc gtttggtgaa atgctgctga gctggaagat
     9661 ggtcatcaaa ataccgacga taatcagccc cgccactggc gtacccgctt tatttacacg
     9721 ggcaaaaatc ggtgggaaca gtccgtcatc ggcagcggct ttcgccgttt gacccgccag
     9781 caacgtccag ccgcccagtg aacctaagca acccgcagct gcgcagaagg aaacaatggc
     9841 cccagcggtg tcacccaacg ccatccgtgc ggcatcaccg aatggcgagg cagaaacgcg
     9901 cagtgcggca ttagggatca tccccataat cgcggtggtg gagagcacat agcaaacggc
     9961 ggcaatcaat accccaccaa tggtggcgat agggacattg cgtttcgggt ttttcaccac
    10021 acctgcggca acggaggcac tttccacacc gatgaacgac cacagcgtaa cgttaagggt
    10081 actttgaatc gcaccgaagg tgcccaggcc gctgacgttc catgccgcca tataggtttc
    10141 accacggaac cagaaccagc caaatacggc aatcccgacg atggggatca gcgccagcac
    10201 agtggcaacg gcctgcacgc gggtgatcat tttcggaccg acaatgttca gcaggacgaa
    10261 gatccacagc accacgacgc aggtgatggt taataccaat ggatctttca gaatcgggaa
    10321 gaagtaactt aaatatccta cgccaatgac caccatggcg atattgccga tccagcaggc
    10381 cagccagtag aggacgttgg tttgataacc gagaaacggg ccaaagcagc ggcgggcgta
    10441 agcgtaagaa ccaccaggac ttgggtcgag gaacgacatt ttggcgtata ccatcgagag
    10501 ccccagcgca ccgataatcg tcaccaacca tccataaatg gcaatcccgc cagtggaggc
    10561 caggtttgca ggtaacagaa aaacacctga ccccataata ttccccgaca ccatcagggt
    10621 gacggggatt aagcccactt tgtgagcatc agcatccgaa gacataatta aactcctgcg
    10681 aaggcgagct tcgtgacaat aaattacgtc atatcatacg cctgcattcg atagttgaat
    10741 tattatcgac cgggttatcg ctgatgccgt tattaatata gctcttcgtt aaggttaaaa
    10801 atcaggcgac agtgcgttct ctgtgctgac taacataatg cagcggcgtc ataccgtaga
    10861 agtctttaaa gacagaaata aagtacgacg tactgttgta gccgcaggac tgtgatacct
    10921 gagagatatt tttaccgtcc atcattaatt catttacggc ataacgcatg cggcaggtgg
    10981 tgattatttg gctataactg gtgttttcgc ttttcaactt tttctttaac agacttgggc
    11041 taagacataa acaactggct accatactaa gattccagtc tttgtgaata tcgctttcaa
    11101 taatttgata aacgctgtca cttacacaat tacgtaacat atacatcatt aatgaaagga
    11161 attttttact gtcgagaaaa cgggatagca ccgtgaataa taatgcgcgt gttcgttcct
    11221 tttctgactc agtttctgcg ggcatcatgc tgtgttgcgc ggcaacgcga aagacgtctg
    11281 gcgtcaggca tggcagggtg agtatgggcg tagcgctacg ttgccatact ggtatttgcg
    11341 agagatcttt attcagaaac cggaggtaat ctttgatgat gtcgtgacta atatgagcaa
    11401 ccaaagtgtt gttaagagag gagacgtcga taatattatt ttcacaattt aataacgcca
    11461 tatggttagc tttaaggcta ataggttctt tcccattcac ccttatccag acatcttttt
    11521 cagtcaataa aacaatacaa ggttggtcgc tgcaaatcct catacatgta ctcctgagtg
    11581 cgaataaaag accagttctc gctaaagcaa agcgataccg ggaaatggtt atataattgc
    11641 atgatgaata taaacatgca taaaaataac gagttttact aatattacaa atttttatag
    11701 taatttctca tgtacatatt tttttacagg ataaataaca ggcaaaaaaa agcgtggggt
    11761 ggggccgcgc aaaaagtatg acatttttgt ttgcagcaat ttttatgaaa acgttgccat
    11821 actcaaaagg cctaaggcca ggcggcgtaa tgttatttaa acaattacgc cttcagcgga
    11881 atagtggtta cgctttcacg cacataacgt ggtaaatacc gtcaataatt tcagtccctt
    11941 cagtttcgtg ttcaaatcta gggaaatggt ggtcccagga ttgcagcgag cgtaaataac
    12001 ttacttgcgg actgttttta tcgccgaagt tttcaccaga cagcagcatc gggattcctg
    12061 gcggatacgg gataactgag tttgccgcga tgcgtcctgg cagattttca atggatacca
    12121 gttcgacatt gttgtcgaca atcgcgttgt acgcttcacg cggggtgatt tccgccaccg
    12181 gcaggccgga ataggcttcg ttcaaccgtg cgccagggtt gttttctttc agccaggcaa
    12241 acatggtgtc acccagatcg tgaatcccca tgttcgcgta agtgtcagga tattgttcaa
    12301 caagttccgg catcacctgc gccagcggtg tgttggcgtc atagtggtgt ttgaaggagc
    12361 aaagggtgtt aaccagagtc ccccatttcc cacgggttac gcccatagag aacaggaaca
    12421 taatttggaa gtcagtggtg cgggtaggca caatgccgtg gcgaccaagc caggcagtga
    12481 ccagcgccgc cggaacaccg gtttcttcca gttcaccatc ttcacccatt cccggagcaa
    12541 ggatgctgac tttaattggg tcgagcatac tccagttatc cggaatatct ttgaagccgt
    12601 gccagctttc gcccggatgc attacccagc agtcctgaac ggtggtcagc agtttggttg
    12661 gtgcgtcagc aaagtcatag gttttgccgg tttgtgggtc ggtgacgact tctttgttcc
    12721 acggtttgaa gaaccagcta ccgtcagcgg tgaactcttt atatagccgc gccatcgcct
    12781 gacggaaatc aaccgcttcg tcaatcactt cctgtgtcag tgacaggccg ctgttgccgt
    12841 ccatcatcga caccgccacg tcgttggatg cgcagatggc atacagcggg gaggtggtgg
    12901 catgcatcat gtaggcctgg ttgaagcggg agaagttaat cgccccacga ccttcacgta
    12961 catgaatata agaagcctgt gacagcgcat tcagcagttt gtggatggag tgggtggcga
    13021 aaacggtagg accgttgtga tcgccaggtt cgccgcgcat ggcatagtga tcggcataga
    13081 tcgggttgaa acgtgcatag ccgtaccagg cttcgtcaaa gtgcagacga tcggaggttt
    13141 tttccagcag atcctgcgct tctttagcgt tataacacac gccgtcatag gtgcagttgg
    13201 ttaccacgca gtaagacggt ttttgcccgg ctttgtcttt ggtcagcggg ctttcactga
    13261 ttttcttttg caaggtttca ggttgcattt cctgcggata gattggcccg ataatgccgt
    13321 agcggttgcg gcttggcacc atatagaccg gtttcgcgcc tgtcagcatc aaaccttgtt
    13381 cgatggattt atggcagtta cggtcaacga ccacgacatc gttatcggtc atgcaagcct
    13441 gcatgatggt gcggttagag ccggaagtac cgacgactac cgaccaggag cgatcggcac
    13501 caaatacgcg tgcggcatat ttttcgcttt cgccaaatgc gccagtatgg tcaagcaaag
    13561 aaccgaggga agttcgttcg atgcccatgt cggtacggaa cagattttca ccatagtagt
    13621 catggtagaa acgtccggcg ggtgttttgg taaaaccaac gccgccctgg tggcctggcg
    13681 ctgcccagga atattcatgg atgtcactat atttcatcag cgcgctgaac agtggcggca
    13741 acagctgctg gcggtagcgg gtcatcgcgg caacggcgcg tccggcgata aagtcggcgg
    13801 tatcttccag aatccaggcg aattcatcga caagctccag cagatcccgg tccattgcgg
    13861 cgagggcttt ttcccgatcg cccaacagga agaccggcac gttttgttgg cgctcatgaa
    13921 gcttaccgat caattgtctg acgttttgat gttcgtccgg atgctccatt tgatagctga
    13981 acatcaggca gtcaatggct tcgtttgaag agagaatggc aaaaccatca tcaaaggagg
    14041 tggatttaat cacggtaaca ttttgctggc ttaaagcatc tgccagacgc tcaacggcgt
    14101 taccgaccca ggtgtcttga tggagaaact cgctttcaac aattaatact ttcatcattg
    14161 cttacccggt tatgaaggaa tcttcgttgc gggcaagtat cttccggcct tttcgggctg
    14221 taaagcgcgt gaaaaacaaa catttttgaa atatttagtt ttataaacaa tacgttatgt
    14281 gattatttta aattattttc actgcttatt atcgacggct aaactatttt tttggctgat
    14341 actgatatcg tctttagccg ggaaacgtct ggcggcgctg ttggctaagt ttgcggtatt
    14401 gttgcggcga catgccgaca tatttgccga acgtgctgta aaaacgacta cttgaacgaa
    14461 agcctgccgt cagggcaata tcgagaatac ttttatcggt atcgctcagt aacgcgcgaa
    14521 cgtggttgat gcgcatcgcg gtaatgtact gtttcatcgt caattgcatg acccgctgga
    14581 atatccccat tgcatagttg gcgttaagtt tgacgtgctc agccacatcg ttgatggtca
    14641 gcgcctgatc atagttttcg gcaataaagc ccagcatctg gctaacataa aattgcgcat
    14701 ggcgcgagac gctgtttttg tgtgtgcgcg aggttttatt gaccagaatc ggttcccagc
    14761 tagagaggct aaatcgcttg agcatcaggc caatttcatc aatggcgagc tggcgaattt
    14821 gctcattcgg actgtttaat tcctgctgcc agcggcgtac ttcaaacggg ctaagttgct
    14881 gtgtggcaag tgatttgatc accatgccgt gagtgacgtg gttaatcagg tctttatcca
    14941 gcggccagga gagaaacaga tgcatcggca gattaaaaat cgccatgctc tgacaggttc
    15001 cggtatctgt tagttggtgc ggtgtacagg cccagaacag cgtgatatga ccctgattga
    15061 tattcacttt ttcattgttg atcaggtatt ccacatcgcc atcgaaaggc acattcactt
    15121 cgacctgacc atgccagtgg ctggtgggca tgatatgcgg tgcgcgaaac tcaatctcca
    15181 tccgctggta ttccgaatac agcgacagcg ggctgcgggt ctgtttttcg tcgctgctgc
    15241 acataaacgt atctgtattc atggatggct ctctttccta gaatatcaga attatggcag
    15301 gagtgaggga ggatgactgc gagtgggagc acggttttca ccctcttccc agaggggcga
    15361 ggggactgtc cgagcatcat gaggccgaaa actctacttt tcaggtaatt tattcccata
    15421 aactcagatt tactgctgct tcacgcagga tctgagttta tgggaatgct caacctggaa
    15481 gccggaggtt ttctgcagat tcgcctgcca tgatgaagtt attcaagcaa gccaggagat
    15541 ctgcatgatg tctgcaccca aaattacatt tatcggcgct ggttcgacga ttttcgttaa
    15601 aaatattctt ggtgatgtgt tccatcgcga ggcgctgaaa acggcgcata ttgccctgat
    15661 ggacattgac cccacccgcc tggaagagtc gcatattgtg gtgcgtaagc tgatggattc
    15721 agcaggggcc agcggcaaaa tcacctgcca cacccaacag aaagaagcct tagaggatgc
    15781 cgattttgtc gtggtggcat ttcagattgg cggttatgaa ccttgcacgg tgactgattt
    15841 cgaggtctgt aagcggcatg gtctggaaca aaccattgcc gatacgttgg ggccgggcgg
    15901 tattatgcgc gcgctacgta ccattccgca tctgtggcaa atttgcgaga acatgacgga
    15961 agtctgcccc gatgccacca tgctcaacta tgttaaccca atggcgatga atacctgggc
    16021 gatgtatgcc cgctatccgt atatcaaaca ggtcgggctg tgccattcgg tgcagggaac
    16081 ggcggaagag ctggcgcgtg acctcaatat cgacccggct acgctgcgtt accgttgcgc
    16141 aggtatcaac catatggcgt tttacctgga gctggagcgc aaaaccgccg acggcagtta
    16201 cgtgaatctc tacccggaac tgctggcggc ttatgaagca gggcaggcac cgaagccaaa
    16261 tattcatggt aatactcgct gccagaatat tgtgcgctac gaaatgttca aaaagctggg
    16321 ctacttcgtc acggaatcat cagaacattt tgccgaatat acgccgtggt ttattaagct
    16381 aggtcgtgag gatttgattg agcgttataa agtaccgctg gatgagtacc cgaaacgctg
    16441 cgtcgagcag ctggcgaact ggcataaaga gctggaggag tataaaaacg cctcccggat
    16501 tgatattaaa ccgtcacggg aatatgccag cacaatcatg aacgctatct ggactggcga
    16561 gccgagtgtg atttacggca acgtccgtaa cgatggtttg attgataacc tgccacaagg
    16621 atgttgcgtg gaagtagcct gtctggttga tgctaatggc attcagccga ccaaagtcgg
    16681 tacgctacct tcgcatctgg ccgccctgat gcaaaccaac atcaacgtac agacgctgct
    16741 gaccgaagct attcttacgg aaaatcgcga ccgtgtttac cacgccgcga tgatggaccc
    16801 gcatactgcc gccgtgctgg gcattgacga aatatatgct cttgttgacg acctgattgc
    16861 cgcccacggc gactggctgc caagctggtt gcaccgttaa aacgcgacta aacgctactg
    16921 cgccggggga tttattccgg cgcacacctc tgacgatacc aataacagaa ggcgggcgtt
    16981 ggtaacagcg acccgatacc ctatgagcat ttcaatgact acaaaactca gttatggatt
    17041 tggagcgttc gggaaggatt ttgcgatcgg cattgtgtat atgtacctca tgtattacta
    17101 caccgatgtc gtcgggctgt ctgtgggttt ggtcggtact ttgtttctgg tggcgaggat
    17161 ctgggatgct attaacgatc cgattatggg atggattgta aatgctacgc gatcgcgatg
    17221 gggtaagttc aaaccctgga tcctgatcgg tacgttggca aactctgtaa tcttatttct
    17281 cctctttagt gcgcatctgt ttgaaggtac tactcagatt gtctttgttt gcgtgaccta
    17341 catcctctgg ggcatgactt acaccattat ggatattccc ttctggtcgc tggttccaac
    17401 catcacgctc gataaacgtg aacgcgaaca actggttcct tatccgcgtt tttttgccag
    17461 tctggcaggc tttgttacgg caggtgtgac gctaccattt gttaattatg tcggcggtgg
    17521 cgatcgggga tttggctttc agatgttcac tctggtattg atcgcctttt ttattgtttc
    17581 aaccatcatc actctgcgca atgtgcatga agtcttttcg tcagacaatc aatcgtctgc
    17641 tgaaggaagc catctgacac ttaaagccat cgttgcgctt atttataaaa acgatcagct
    17701 ttcatgcctc ttgggtatgg ctcttgctta taatgtagcc agcaacatta ttaccggatt
    17761 tgctatctat tatttctcat atgttatcgg tgatgcggat ttgttcccct attatctgtc
    17821 gtatgcggga gctgctaacc tggtgacgtt agtattcttc ccacgcttag ttaaatcatt
    17881 atcccgacgc attttatggg ccggagcatc tattcttccg gtgttaagct gtggtgttct
    17941 cctgttaatg gcattaatga gctatcacaa cgtcgtcctc attgtgattg cgggtatttt
    18001 gctgaatgtg ggaacggcgc ttttctgggt attacaggtc atcatggtgg cagataccgt
    18061 tgattacggt gaatataaac tgcacgtacg ctgtgaaagc atcgcttact ccgtgcagac
    18121 tatggtggtg aagggcggtt cagcctttgc ggcttttttc attgcggttg tgttagggat
    18181 gattggctat gtaccgaatg ttgaacagtc tacgcaagcc ctattaggta tgcagtttat
    18241 tatgattgct ctaccgactc tgtttttcat ggtaacgctg attctctact tccgtttcta
    18301 tcgcctcaat ggcgacacgc tgcgcaggat ccagattcat ctgctggata aatatcgcaa
    18361 agtaccgccc gagcctgttc atgctgatat tccggtcggt gcagtgagtg atgtgaaagc
    18421 ctgacgtgga caatagctaa cgaaaatggc ctgatgcgat atgtttatca ggccacgtct
    18481 taatgtaatg ctttggattt gcaaattttg tagaccggat aaggaattca cgccgcatcc
    18541 ggcatcaaca aagtgcacgt tgttatccag ttatcaagcc atagcgccgt agctggcggc
    18601 aatgaaagcg agcgagcaat atcactgtcg ttaatgcgcc aagcagcgcg cagaacatat
    18661 cggattgcgt gtcccactgg tcgccctggg taccgagaaa atcatccgct ccctggccca
    18721 tcgccagtgc cgcccaccac tcgattaatt catacatcgc gcttatcgcc agcgccacgc
    18781 aacagaccag aaacgccacc attttacgtc cgcgcacgta catcccgcga acgagaattt
    18841 ctcgtgccac cagtgcaggc accagcccct ggaaaaagtg ccccagcttg tcatacggat
    18901 tacgactcaa ccccagccat tcctgcacct caaaaccaac ggggactttc gcgtaggtgt
    18961 actgcccgcc taccatcagg atgatggcgt gaaagaagat gagcgtatag agcagcagcg
    19021 ttaacgggta acgtctggcg gtggcaagta gtagcggcac gacgataatc accggtgtca
    19081 cttccagcag ccaggtgagt ttgtcatggg ccgaaatgcc ggtataaatc aggattaacg
    19141 ttagcgccag tgcgccagtg ttaagaatta acggcttgag tgtgcgggtc atggtgagtt
    19201 cgcaagtcag ggaaaatact gactattcac cagcaagcat gaaattacaa tcgcaaaaga
    19261 ggtggcccag ggggatcacc tggcagcatg ctgccaggcg ctgggccgaa gaggttactt
    19321 agtgcagttc gcgcactgtt tgttgacgat ttgctggaag aagtcgttac ctttgtcatc
    19381 gaccaggata aacgccggga aatcttctac ttcgattttc cagatagctt ccatacccag
    19441 ctccggataa gcgacgcatt ccagatgctt gatgctctgc tgcgccagta ccgccgccgg
    19501 accgccgatg ctaccgaggt agaagccgcc gtgtttatga cacgcgtcgg taacctgctg
    19561 gctgcggttg cctttcgcca gcatgatcat gctgccgccg tgggattgca gcagatccac
    19621 ataggagtcc atacgacctg cggtggttgg gccaagtgaa cctgatggat aaccggcagg
    19681 ggttttcgcc ggacccgcgt agtagatcgg gtgatctttg atgtactgcg gaagttcttt
    19741 accggcgtca atcagctctt tcagcttggc gtgtgcaata tctcgaccca caataatggt
    19801 gccagtgagc gacaaacgag tggaaaccgg atattgcgaa agctgggcga ggatctcttt
    19861 catcgggcgg ttaaggtcga ccttcaccgc ttcgccttca ccggcctgac gcagttcttg
    19921 tggaatgtac tgacctagat tgtgttccag tttttcgatc cagatacctt cgcggttgat
    19981 tttcgcttta atgttacggt cagcggagca ggagacgccc atgcctaccg ggcaggatgc
    20041 gccgtgacgt ggcagacgga taacgcgaat gtcgtgcgcg aagtatttac cgccaaactg
    20101 cgcgccaaga ccgagtttct gggcctcttc cagcagttcc tgttccagct ggacatcgcg
    20161 gaacgcctga ccatgttcgt tcccttccgt cggcagttca tcgtaatagt gagcgcttgc
    20221 taatttgacg gttttcaggt tgttttccgc agacgtaccg ccaatcacaa acgcgatatg
    20281 gtacggcggg caggctgcag taccgagggt acgcattttc tcgacgagga agtttttcag
    20341 tttgccggga gtcagcaggg ctttggtttc ctggtagaga tacgttttgt tggcagagcc
    20401 gccgcctttc gcaacgcaaa ggaatttgta ctcatcgcca tctaccgcgt acaggtcgat
    20461 ttgcgcaggc aggttagtgc cggtgttgac ctctttgtac atgtccagcg ccgcgttttg
    20521 cgagtagcgc aggttatctt cgatataggt gttatagacg cctttcgaca gcgcttcttc
    20581 atcaccgcca ccggtccaca cgcgctggcc ttttttaccg acgatgatcg cggtgccggt
    20641 atcctggcag gtcggcagca cgcctttggc ggcgatttcg gagtttctta agaattgcag
    20701 tgccacgtac ttgtcgtttt cgctggcttc tggatcgtga agaatagccg caacctgttt
    20761 ctggtgtgcc ggacgtagca tgaaagaagc gtcgtgaaag gcttgctgcg ccagcagggt
    20821 cagggcttct ggttccactt tcaggatggt ttcgccgtcg aagtcggcaa cgctaacgta
    20881 atcggaagtg agtagatagt attcggtatt gtctttcccc atcgggaaag gtgcctggta
    20941 gataaagggt ttgtttgaca tagcttccag cctgtaactc tgtatttgtt attcgaaaat
    21001 ggcgtgccgc gtagtcacgg gcggcacgca aagtgcattt ataagaaccc gtacatcgcg
    21061 gcgaagatcc agccgaagac gcacgatacg ctcacaccaa tcaaccccgg cagaataaaa
    21121 ctgtggttga tgacgaagcg accgatgtgg gtggtgccgg aacggtcaaa ctgaatcgct
    21181 gccagatcgc tcggataagt cggcaggatg taataaccgt agcaagccgg tgctgaagcc
    21241 acgatgtatg ccggatcaac gccgatcgcc agcgcgaccg gaacaatcgc cgccagcgcc
    21301 gcagcctgag agtttacaaa cttggaaacc agcagcagaa caatggcata cgcccacgga
    21361 tactctttca ccatttcacc cagtacgccc tgaatttcag acatatgcgc accgaacatg
    21421 gtttctgcca tccatgcgat accgtacacc gccacgatgg cgatcatacc ggaacggaag
    21481 acttcgtttt ttgagataga cgcgggattg gttttggtca ggataataat cagcgccccg
    21541 gtcagcagca taaacatctg aataaccagt accatcgaca gcggtttgcc gccgaaggat
    21601 ggacgcaggt ccgaatcagc accaagaagg gcgactacag cgattgcccc gaggaaaatc
    21661 cacattgcca gccagttgct tttcggcagt tttttatcca gcagcgtcgc ggtatcaccg
    21721 taaacatact cacggttttc cggtacggag atgaatttct ggaactcttc gtctttatcc
    21781 agatctttac cgcggaacca gctgaagata ccgatcgcca ggataccgat taacgtcgat
    21841 ggaatggtga ttgccagcag atcgaggaac tcaagatggc gaccatcaaa ggtgacatta
    21901 cccagcatcg caaccagaga cacgaccgca accgacaccg gactggcgat aatccccatc
    21961 tgtgcaccga tagaacttgc cgccatcgga cgttccggac ggatgttgtt cttaatggcg
    22021 acgtcgtaga tgatcggcag aatggtgtaa accacatgac ccgtaccgca aagaatggtc
    22081 agtgtacagg tcacaaacgg cgcgacaatt gagacatatt tcgggttgcg gcgcagcagc
    22141 ttctcggcaa tttgcagcat gacatcaaga ccgcccgaag cttgcaaggt cgccgatgcc
    22201 gccaccaccg caatgataac cagcatgaca tcaactggtg gtttacctgg ctgaaggtgg
    22261 aagacgaaga ccagaatgac cagaccgata ccgcctaata aacccagcgc gataccaccc
    22321 tttctggcac cataaaacag acatattagt attatgataa gttggatagt aaataacatg
    22381 tgtgaaccct cgcgataatc ctatttaaat ttttgctgaa tagatcacag tcacgttctg
    22441 ttttgtatga actgttttca gagcctgact aaatatccgt ttggtcgtta ccggctttag
    22501 caaatacctc acagtgaata ttggctgatt aaagcagcgg caaatttatg cactgtgata
    22561 agcggctttt tcagagagaa aaactctctg gcgatgggtt tatagtttgt actgcgcaga
    22621 tgttaatgtt ttgtttgctg ttatcttatt gatatttatc ggtctatttt ttgttctcag
    22681 ggcagatatc attaggttta tagattaatc tgatctaccc atttgtgggt aaaaatacac
    22741 ataatgcggg tgacataata gttaattaac ttttgttatc gttttgaaat taaaaacacc
    22801 gttcatctga agagatatta atttttaacg atgatggagg gataattata tttgatctgg
    22861 cacaagtttt actgattaac gatgtaactt gtgccagggg tattttgcat tacggtaata
    22921 attattactt acagataacc agcgatccgt tattggcaat attgtttcag cagtgagtag
    22981 tgttctgcct gaatacggta acggtaaacc ggacgccccg tgacgccata atggatactg
    23041 gtgaacaaga tgtggcagtt gaccagccag atgaggtatt ttcggcagga aacacgcgag
    23101 atgttaacct cgttagccag ctcgtcggtt gaaaactcat agtcctgatg cgcgtcaatc
    23161 cactggcaca gcgtgcgtaa cgtctgcggc gttaagcctt ttggcaagcg acgaggatcc
    23221 tgttcgttgg agctgctgcc gtggattagc tgatcaagct cggcctggtc ataatactga
    23281 tgtttttcca gcgccatttt cttttgccgc cagccggtga gcgcctcttc aaagcgggaa
    23341 gcctggaagg gtttgatcag gtaatccacg acaccgtaat gcagcgaatc tttaatggtt
    23401 gccgcatcgg ctgcggagga gatgacaatc acatcacttt tgcaacgcgc gttatgcagg
    23461 acaggcagta aatcgagccc gttctctttt tgcatataga tatcgagcaa tatcaggtcg
    23521 ataggcgcat cgctattgaa gataatctct ttggctttct ccagcgtcga ggctgttcca
    23581 cagcattgaa agcctgggat ttgtgctacg tatcggcgat tcagctccgc gaccattgcg
    23641 tcgtcatcga taattaatac attgatcatc tgttcgacct ctccccgtcc cagggtatct
    23701 ggacaaaaaa ttgtgtgaaa atccccggtt ccgattccac ggcgatgctg ccgccgagat
    23761 tttctacctg ttgtttgaca agtgctaaac cgacgcctcg ctcgcttcct tttgtcgaga
    23821 cacctttgtc aaaaatgtga tcgattttgt cgggtgcgat ccccggtcca tcatcgttaa
    23881 cttcgcagtg cagccagccg tgacggtagt gcaatgttac gctaatttcg ccaccgggtt
    23941 ccggccctaa tgcttccagc gcgttttcta tcagatttcc caatgtggta atcagcgtcg
    24001 cgacctggtc ctcactgccg ctgtctggca gctggctttc actgtttaaa atcagcgtat
    24061 ggcctaaatc ggtcgcgcgg ttaatcttgc tgattaaaaa accagcgata accggagatt
    24121 tgatcttacc cagcagagag ccaatctctt cctgatagtt attggctgtt ttgagaatgt
    24181 aatcttccaa ctgcttataa ctcttcagat gcaataatcc gagaatcaca tgcaatttat
    24241 tcataaattc gtgggatcgt tcacgaagtg cgtcagcata gttgaccaga ccgtcgagtc
    24301 gctgcatcag cttacgaact tcagttttgt ccctgaaggt tgaaatggca ccgatgataa
    24361 cgccattact gcgcaccgga acggtgttga tcagtaatag ccggtcttta atcgtaatct
    24421 cttcgtcgcg gcgcggcgta ccgtcgcgta acacttccga gacatctacc acctgtgacc
    24481 atgagtggct gagcgtcgac agtttctcat cgtcctgcga cttacggtaa ttcagcaatt
    24541 cttgtgcggc atcgttgatc agcgtgacct cgccgcgatc gtccacggca acgacgcctt
    24601 ctttgataga ctgcaacatg gcctggcgtt gctcaaacag cgtggagatt tcgtagggtt
    24661 ccaggccgaa aaggattttt ttcagtacct taaccagaat gcaggtgcca atcagtccga
    24721 ccagcatgcc aaataatacc gaccagataa tgctccagcg actgtcattg atctgttggg
    24781 tcacgcggct taactcaagg ccgatcgcca ccacgccaat ttgtttatga ttttcatcgt
    24841 agatgggggt aaatacgcgt aaagcctgcg ccagaaaacc gcgattgata gcgacatttt
    24901 cttcgccatt cagcgcatta aggatgtcat cacctttaaa tggctgacca atacgctggg
    24961 cttcaggatg tgagtagcga agactttgca tatcggtaac gacaataaac agcagatcgt
    25021 tgcgtttgcg cacggcttcc gcgatggcct ggatgccact ctcctgcggt tttttctgca
    25081 agccctgacg gatttccggc gagtcggcga gggtacgcgc cactgccagt gccttgttgg
    25141 ccagcccgtc tcgcgtcata tcactgattt gcgagaagta aatcagatgc accaccaata
    25201 gcaccgagaa cagtaccgcg ctgaccatta agatcacagt ggtactcaat ttcatcggac
    25261 gtttgcgtaa tatgtggtag ggcaatgaat gtctcatcag cttccttgtg tgacaaattt
    25321 cttaagcatt atctctgatg aggcgggtaa ttcaaaggga gtaaaaatga ttggctatat
    25381 aggggaagag actctggcaa cggaaactgc cagtgctgta tgaagattcc ggggctatgc
    25441 ttatagcgat aatcatactg atgagagagg gaaggtcatg gatcaggcgc tactggacgg
    25501 gggttatcgc tgttataccg gcgaaaagat cgatgtctat ttcaacactg cgatatgtca
    25561 gcattctggc aattgcgtac gtggcaacgg caagttattt aatctcaaac gaaagtcgt
//
LOCUS       DAZPAB010000038        24653 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZPAB010000038 DAZPAB010000000
VERSION     DAZPAB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.038"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(1..386)
                     /locus_tag="ACHUPG_001418"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="ACHUPG_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="ACHUPG_001419"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="ACHUPG_001419"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR0861093.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="ACHUPG_001420"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="ACHUPG_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR0861094.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="ACHUPG_001421"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="ACHUPG_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR0861095.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="ACHUPG_001422"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="ACHUPG_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR0861096.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="ACHUPG_001423"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="ACHUPG_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR0861097.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="ACHUPG_001424"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="ACHUPG_001424"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR0861098.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="ACHUPG_001425"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="ACHUPG_001425"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR0861099.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="ACHUPG_001426"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="ACHUPG_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR0861100.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="ACHUPG_001427"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="ACHUPG_001427"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR0861101.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="ACHUPG_001428"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="ACHUPG_001428"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR0861102.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="ACHUPG_001429"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="ACHUPG_001429"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR0861103.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="ACHUPG_001430"
     CDS             10748..11212
                     /locus_tag="ACHUPG_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR0861104.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="ACHUPG_001431"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="ACHUPG_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR0861105.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /gene="selO"
                     /locus_tag="ACHUPG_001432"
     CDS             12236..13672
                     /gene="selO"
                     /locus_tag="ACHUPG_001432"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR0861106.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="ACHUPG_001433"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="ACHUPG_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR0861107.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="ACHUPG_001434"
     CDS             complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="ACHUPG_001434"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR0861108.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="ACHUPG_001435"
     CDS             complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="ACHUPG_001435"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR0861109.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16367..18745
                     /gene="ppsA"
                     /locus_tag="ACHUPG_001436"
     CDS             16367..18745
                     /gene="ppsA"
                     /locus_tag="ACHUPG_001436"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR0861110.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="ACHUPG_001437"
     CDS             complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="ACHUPG_001437"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFR0861111.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20505..20798)
                     /locus_tag="ACHUPG_001438"
     CDS             complement(20505..20798)
                     /locus_tag="ACHUPG_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR0861112.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20795..22084)
                     /locus_tag="ACHUPG_001439"
     CDS             complement(20795..22084)
                     /locus_tag="ACHUPG_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0861113.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22140..23078)
                     /locus_tag="ACHUPG_001440"
     CDS             complement(22140..23078)
                     /locus_tag="ACHUPG_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFR0861114.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23098..23862)
                     /locus_tag="ACHUPG_001441"
     CDS             complement(23098..23862)
                     /locus_tag="ACHUPG_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFR0861115.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24181..>24653
                     /locus_tag="ACHUPG_001442"
     CDS             24181..>24653
                     /locus_tag="ACHUPG_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFR0861116.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALAYTLA"
BASE COUNT         6103 a         5950 c         6484 g         6116 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgcctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat aattgatata acaatgattc ctattatcaa tattatggca
    13921 gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca
    13981 tcaggaatgg gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag
    14041 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    14101 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    14161 aatctccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    14221 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    14281 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    14341 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    14401 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    14461 ggcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga
    14521 agccatttcg cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat
    14581 taaatcagca ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc
    14641 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    14701 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
    14761 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    14821 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    14881 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    14941 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    15001 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    15061 tttgttgctt ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca
    15121 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    15181 acaaaccaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    15241 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    15301 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    15361 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    15421 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc
    15481 ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt
    15541 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg
    15601 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    15661 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    15721 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    15781 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    15841 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    15901 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    15961 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    16021 aacagcatta tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat
    16081 taaaagcgca cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat
    16141 tttttataaa aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa
    16201 attgtcgaat agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
    16261 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
    16321 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
    16381 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
    16441 gggcaaaaat gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt
    16501 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
    16561 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc
    16621 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
    16681 aaacgccata cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc
    16741 ggtacgctcc tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga
    16801 aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
    16861 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
    16921 cgtggcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt
    16981 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
    17041 gggccttggg gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa
    17101 accgacactg gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
    17161 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
    17221 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
    17281 ggccgtacaa attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg
    17341 ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
    17401 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
    17461 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
    17521 catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat
    17581 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
    17641 cgctcgtgaa ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa
    17701 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
    17761 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa
    17821 agtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga
    17881 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
    17941 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
    18001 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
    18061 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
    18121 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
    18181 cggcttccgt ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga
    18241 gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat
    18301 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg
    18361 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc
    18421 cttgctggcc gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat
    18481 gacgcagctg gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga
    18541 gcgcaacgat gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg
    18601 caaatatgtc gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt
    18661 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
    18721 aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta
    18781 tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg
    18841 tataatatct tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt
    18901 ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa
    18961 agccgccatc tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca
    19021 tgatcgttca cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg
    19081 atgctgcaat aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat
    19141 aatatctttt ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc
    19201 gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa
    19261 ataccccata aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa
    19321 ggtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt
    19381 atgcataaag cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt
    19441 ggaaccataa acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt
    19501 ggggattgtg gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt
    19561 ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg
    19621 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
    19681 agcgccaatt aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg
    19741 cgcaggcatc ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc
    19801 actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga
    19861 ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat
    19921 attgtcggcg ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac
    19981 gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg
    20041 acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac
    20101 ccacaccagt tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt
    20161 caggcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg
    20221 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
    20281 gagcgcgcta taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat
    20341 cgcacgagcg gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg
    20401 acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg
    20461 aaacagaaca tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa
    20521 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc
    20581 acccgacagg tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca
    20641 tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg
    20701 ggattttccg tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
    20761 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
    20821 catctttcag caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta
    20881 ccggctggtt tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc
    20941 gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct
    21001 gcatatcacg catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg
    21061 cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg
    21121 caatggctaa atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt
    21181 caccaacgat catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg
    21241 gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg
    21301 ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac
    21361 ccaatccaca aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc
    21421 ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa
    21481 tattaaagcg atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag
    21541 cgtaatgatg cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa
    21601 cgccatcagc cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag
    21661 tgaccttgtt tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc
    21721 ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag
    21781 atgcgtgttg aggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt
    21841 cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa
    21901 agcctggaat gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac
    21961 atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca
    22021 gtgcggcaac gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg
    22081 acatgcgtta tgctcccttc ctgctgcgtt tacagcggga gatgtggttg aaaggggaat
    22141 tagcggctca actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg
    22201 tagtcggcat agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt
    22261 acgccgttgc cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga
    22321 tcggatttca gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg
    22381 ccttcggcaa ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg
    22441 accattttca ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct
    22501 ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga
    22561 cgcggagcaa cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac
    22621 ggttcctgaa caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga
    22681 gcgaacgcta acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc
    22741 accgccgtgg catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga
    22801 gctttaccac gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg
    22861 gcagcaatgc tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca
    22921 taaatacatt ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg
    22981 gcatacacct gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga
    23041 ttatcgctaa agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca
    23101 gttcagggct ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc
    23161 gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag
    23221 tggcgcgctc tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc
    23281 cgcgccgaga atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca
    23341 gagcacggct ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat
    23401 caccagtgta ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag
    23461 aatttctccg accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa
    23521 gatcagtaaa tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag
    23581 aggcagtgca tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga
    23641 tagcacgtct ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc
    23701 tatctcatcg tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg
    23761 gctgattttg gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc
    23821 ctgttcttca ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg
    23881 catacggaac catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt
    23941 gcgggggggg gggcgacgga gcaagctgtt ttccttgcca acaatcttgc aaaaaaaatg
    24001 cgattgctaa aacaaaagtg caatttgcgg caatatagtt attccatgac cgtacaatct
    24061 tgcattcagt gtaaataatc ttgtttagct ctcgctaaac gcggttgtat aaccgcaata
    24121 cacgccgtat tttatccggc atatattgtt tttgcttgct catggaatag ggaggttgtc
    24181 atgtatcaac gctgttttga taatgccagt gaaacgctgt ttgtcgccgg taaaacgcca
    24241 cggctttcac gttttgcatt tagcgatgat ccaaaatggg agtctggaca tcacgttcat
    24301 gacaatgaaa ccgagctgat ttacgtcaag aaaggggttg caaggtttac catcgattct
    24361 tcgttatatg tcgcacatgc ggatgacatt gtggtgatag aacgcggcag gctgcatgcg
    24421 gtggcctctg acgttaacga tccggcaacg acgtgtacct gtgcgctgta cggctttcag
    24481 tttcaggggg ctgaggaaaa tcagttactg caaccgcatt cttgtccggt aattgccgcg
    24541 gggcagggaa aagaagtcat aaaaacctta tttaatgagc taagtgtgat tttgccgcaa
    24601 agtaaaaata gccaaacatc ttcgttatgg gatgcattag cctatacatt agc
//
LOCUS       DAZPAB010000039        24589 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZPAB010000039 DAZPAB010000000
VERSION     DAZPAB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.039"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(2..94)
                     /locus_tag="ACHUPG_001443"
     tRNA            complement(2..94)
                     /locus_tag="ACHUPG_001443"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="ACHUPG_001444"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="ACHUPG_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR0861117.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="ACHUPG_001445"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="ACHUPG_001445"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR0861118.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="ACHUPG_001446"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="ACHUPG_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR0861119.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="ACHUPG_001447"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="ACHUPG_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR0861120.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="ACHUPG_001448"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="ACHUPG_001448"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR0861121.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="ACHUPG_001449"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="ACHUPG_001449"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR0861122.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="ACHUPG_001450"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="ACHUPG_001450"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: protein motif:HMM:NF015565.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="ACHUPG_001451"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="ACHUPG_001451"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR0861123.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="ACHUPG_001452"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="ACHUPG_001452"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR0861124.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="ACHUPG_001453"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="ACHUPG_001453"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFR0861125.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="ACHUPG_001454"
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="ACHUPG_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR0861126.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="ACHUPG_001455"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="ACHUPG_001455"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR0861127.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="ACHUPG_001456"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="ACHUPG_001456"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR0861128.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="ACHUPG_001457"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="ACHUPG_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR0861129.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="ACHUPG_001458"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="ACHUPG_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR0861130.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="ACHUPG_001459"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="ACHUPG_001459"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR0861131.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="ACHUPG_001460"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="ACHUPG_001460"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR0861132.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="ACHUPG_001461"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="ACHUPG_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR0861133.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="ACHUPG_001462"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="ACHUPG_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR0861134.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20948..21019)
                     /locus_tag="ACHUPG_001463"
                     /pseudo
     CDS             complement(20948..21019)
                     /locus_tag="ACHUPG_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="ACHUPG_001464"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="ACHUPG_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR0861135.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="ACHUPG_001465"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="ACHUPG_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR0861136.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..>24589)
                     /locus_tag="ACHUPG_001466"
     CDS             complement(23682..>24589)
                     /locus_tag="ACHUPG_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HFR0861137.1"
                     /translation="TGIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGSVWFRTG
                     HRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVA
                     RLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVALAI
                     CCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKGDR
                     LPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEG
                     SALLFRRMALVELAVLVVIIVSRGA"
BASE COUNT         5799 a         6919 c         6306 g         5565 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtgga ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagattgttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgggcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatc ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactag catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agttgcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacactcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 aggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgtta
//
LOCUS       DAZPAB010000040        24574 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZPAB010000040 DAZPAB010000000
VERSION     DAZPAB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24574
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.040"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(14..520)
                     /gene="mug"
                     /locus_tag="ACHUPG_001467"
                     /pseudo
     CDS             complement(14..520)
                     /gene="mug"
                     /locus_tag="ACHUPG_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
     gene            645..720
                     /locus_tag="ACHUPG_001468"
     tRNA            645..720
                     /locus_tag="ACHUPG_001468"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:678..680,aa:Ile,seq:cat)
     gene            complement(774..1519)
                     /gene="nfeF"
                     /locus_tag="ACHUPG_001469"
                     /gene_synonym="yqjH"
                     /pseudo
     CDS             complement(774..1519)
                     /gene="nfeF"
                     /locus_tag="ACHUPG_001469"
                     /gene_synonym="yqjH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
     gene            1796..2419
                     /gene="nfeR"
                     /locus_tag="ACHUPG_001470"
                     /gene_synonym="yqjI"
     CDS             1796..2419
                     /gene="nfeR"
                     /locus_tag="ACHUPG_001470"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR0861138.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(2573..4093)
                     /gene="aer"
                     /locus_tag="ACHUPG_001471"
                     /pseudo
     CDS             complement(2573..4093)
                     /gene="aer"
                     /locus_tag="ACHUPG_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            4511..5890
                     /gene="ygjG"
                     /locus_tag="ACHUPG_001472"
     CDS             4511..5890
                     /gene="ygjG"
                     /locus_tag="ACHUPG_001472"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR0861139.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5932..6263)
                     /locus_tag="ACHUPG_001473"
                     /pseudo
     CDS             complement(5932..6263)
                     /locus_tag="ACHUPG_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            6481..7464
                     /gene="ebgR"
                     /locus_tag="ACHUPG_001474"
     CDS             6481..7464
                     /gene="ebgR"
                     /locus_tag="ACHUPG_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR0861140.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            7647..10733
                     /gene="ebgA"
                     /locus_tag="ACHUPG_001475"
     CDS             7647..10733
                     /gene="ebgA"
                     /locus_tag="ACHUPG_001475"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFR0861141.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            10730..11179
                     /locus_tag="ACHUPG_001476"
     CDS             10730..11179
                     /locus_tag="ACHUPG_001476"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR0861142.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            11242..12675
                     /locus_tag="ACHUPG_001477"
     CDS             11242..12675
                     /locus_tag="ACHUPG_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0861143.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            12809..13880
                     /gene="ygjJ"
                     /locus_tag="ACHUPG_001478"
                     /pseudo
     CDS             12809..13880
                     /gene="ygjJ"
                     /locus_tag="ACHUPG_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            13897..16248
                     /gene="ygjK"
                     /locus_tag="ACHUPG_001479"
     CDS             13897..16248
                     /gene="ygjK"
                     /locus_tag="ACHUPG_001479"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR0861144.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATQEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            16474..18492
                     /gene="fadH"
                     /locus_tag="ACHUPG_001480"
     CDS             16474..18492
                     /gene="fadH"
                     /locus_tag="ACHUPG_001480"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR0861145.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18537..18953)
                     /gene="higA"
                     /locus_tag="ACHUPG_001481"
     CDS             complement(18537..18953)
                     /gene="higA"
                     /locus_tag="ACHUPG_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR0861146.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(18950..19264)
                     /gene="higB"
                     /locus_tag="ACHUPG_001482"
     CDS             complement(18950..19264)
                     /gene="higB"
                     /locus_tag="ACHUPG_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR0861147.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(19549..20685)
                     /gene="rlmG"
                     /locus_tag="ACHUPG_001483"
     CDS             complement(19549..20685)
                     /gene="rlmG"
                     /locus_tag="ACHUPG_001483"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR0861148.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            20770..21273
                     /locus_tag="ACHUPG_001484"
     CDS             20770..21273
                     /locus_tag="ACHUPG_001484"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR0861149.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            21350..22042
                     /locus_tag="ACHUPG_001485"
     CDS             21350..22042
                     /locus_tag="ACHUPG_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR0861150.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            22121..23119
                     /locus_tag="ACHUPG_001486"
     CDS             22121..23119
                     /locus_tag="ACHUPG_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR0861151.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            23390..24355
                     /gene="alx"
                     /locus_tag="ACHUPG_001487"
     CDS             23390..24355
                     /gene="alx"
                     /locus_tag="ACHUPG_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR0861152.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         5876 a         6464 c         6406 g         5828 t
ORIGIN      
        1 ccggtgacgt tttttatcgc ccacgcacta ccagcgcctg gtccagctcg cgatacgctt
       61 caaccagttt ctccagtgaa acgcgactta aaccgctggg atttggcagc acccaaatct
      121 gcgtcgaacc aatggtgagc gtttgtttcc cccactgtgc accgcgctag ctgaatccct
      181 gttcatatgc ttgtttgccc agaatagcca acgcctgcgg ctgataatct tcaatttttt
      241 caatcagctt acgcccacct gcgtgcagct cctgctttga aacttcattg gcttgcaccg
      301 ttggacggtc taccagtttg gtgatgccac aacgataatc cagcagatgc tgtgcctcct
      361 gcggcttcaa ctgacggtcg gtaaacccgg cctgatatat caccttccag aagcgatttg
      421 ccggatgagc aaagggaaaa ccagtcccgg cggatgaaag cccagggttg ataccgcaaa
      481 acacgacccg taaccctgga gccaaaatat cctcaaccat atttactcct ttctaacatc
      541 accaggaaaa gtataacgga ttgaaaatac attgtttata aaaacagcag gcgcgcggta
      601 atggctggat tgcggcacgg agttacttta taatccgcta ccatggcccc ttagctcagt
      661 ggttagagca ggcgactcat aatcgcttgg tcgctggttc aagtccagca ggggccacca
      721 gatatagcaa aggctgacga gaaatcgtca gcctttttct ttttatatat cagttacttt
      781 gcgtgccagt aagccgctgc acgtacccgc tgtgggtcat actgttccgc ttcaaagcgg
      841 cggcttaaat tcttaacgac tttaccttcg ccggttatcc agatgaagta atcatcggca
      901 gggatttgca tctgcgccag acgcgcatct accgcctgct catcatgtgc cagccattcg
      961 atattaaaac catcaaggtg cgcgagataa tcctgacagg cgttatcccg cacgctaacc
     1021 agcgcactaa cttgcggttt aacggcaagt ttgctcaacg tttccaggcg gcggcgcagt
     1081 gcgggcattc cggattcatc gcagacatac agctgatacg cgtaatcttc cggcaccacc
     1141 agcgaaccgc gcggacctgc caccgtaagt ttatcgccca gccgctggcg accccaccgt
     1201 cgtgaataaa gaaatcgatc gccagttcat ggcgtagttc gtcatacagt ggcgtatagt
     1261 cacgcgacgg tgggcgtggt ccttccggcc agacgatgct ctcttccgtt accgttggcg
     1321 gcacaaagtg agcgtcaggt tgaggaaaga agagtttgct gtgatcgtca aagccgcgcg
     1381 atgtaaaacc gtccaacgcc tcgccgccaa ggacaatgcg ctgaaaaccg gcgctgatgc
     1441 gctcaacgcg tagcacagtc agttcacgga agcgcagatc attgcgaacg cgctgcgggt
     1501 agcggggggt gttattcatt gttatcgcct tcgtgatggt aatcagatat atctaaataa
     1561 aattcgcaaa tgataatgat tgttaatcat gataaatgca agcgatttgt agaactgata
     1621 tgtctgaagt atgtgaaggc attcagtgcc atagcaatgc tgccggatgc ggcgtgaacg
     1681 tcttatccgg cctagatgtg aggtgcttcc acgtttaaaa atcatttttg tacttgcagt
     1741 aatgctcaca tcagatataa attagatata tctaatcaag caaaaggagg ctgatatgag
     1801 ccatcatcac gaagggtgtt gtaaacatga aggccagcca cgccatgagg gctgctgcaa
     1861 aggtgagaag tcagaacacg agcactgcgg acacggtcac cagcatgaac acggtcaatg
     1921 ttgcggtggt cgccacggtc gcggcggcgg tcgtcggcaa cgtttctttg gtcacggtga
     1981 attgcgtctg gtgattctgg atattctctc gcgcgatgac agccacggtt acgaattgat
     2041 taaagcgatt gagaatctaa cccaggggaa ttacacccca agcccgggcg tcatctaccc
     2101 gacgctggat tttctgcagg agcagtcgct gattaccatc cgcgaagagg aaggtggtaa
     2161 gaagcagatt gcgctgaccg aacaaggcgc gcagtggctg gaagaaaacc gcgaacaagt
     2221 ggagatgatt gaagaacgca tcaaagcgcg ttgcgttggc gcggcgctgc gccagaaccc
     2281 gcaaatgaag cgggcgctgg ataattttaa agcggtgctg gatttacgcg tcaaccagag
     2341 cgatatcagt gatgcacaaa taaaaaagat cattgcggtg atcgaccgcg ccgcttttga
     2401 tattacgcaa ctggattaat cgccgcatcc gccagtggcg cggtgcaatt gccggatgcg
     2461 acgcttgacg cgccttatcc ggcctacacc cgctacacac cccgcaggcc tgataagatg
     2521 cgccagcatc gcatcaggca ttgtgctcca accgccggat ccggcatacc gattaatgca
     2581 gcaccgtcac cgcgtcttcc agtcggctgg cgcggtgttt caccatcgcc gacacctgcg
     2641 cactctcttc caccagctcg gcatttttct gggtgatcag gttaagctca tccactgcac
     2701 gggtcaggct ggaaagccca tcggcctgtt ccagcgttga atggctaatc tgggcgatca
     2761 actgggtgac gtttttcacc tgtgccacaa tatcttccat cgtccgtccg gcggcgtgta
     2821 cctgctgcga accggattgc accttatcag cactggcatc aatcagcttg cgaatatcgt
     2881 tggcagcatt agcgctgcgg ctggctaaat gacgcacttc ccctgccacc actgcaaaac
     2941 ctttgccctg ttcgccggca cgcgccgctt ccaccgccgc attcagggcc agaatattgg
     3001 tctgaaacgc aatatcgtta atcagcgaag taatggtgcc aatgcgctag gtactgtcgg
     3061 cgatatcgtc cattgtcttg atcaccgtgg tcatcgcctc cccaccctgc actgccgcat
     3121 tactggcggt gatagaaagt ttatcggcag ccgacgccgt ggcagagttc tgtttcaccg
     3181 acgccgccat ttggttcatg gtcgccaccg tttgctgaac gttatcaact gtctgctggg
     3241 tatgttcgtt cagttcatcg gtgcctttcg ccagcgtctc actgccattt ctgacactgg
     3301 acacctggct tgagacatcg ttaattagcc aacggcacat caggccaagc tgccctaccg
     3361 cgcgtaatgt cagccccagc tcatcgctgc gattcaggtg ctcaacacta ttacgctctc
     3421 cggtcgccac cttcagtgcc tgacgggcga cattttctat cgggcgcaca atctgccatt
     3481 caaaacaagc gcttgccaac aataccacta acacacagag gaaatacgtc accaccgggg
     3541 cagcaacaaa ccaaagcatg gccgccagca agataaacat cagggtcatc actccacgcg
     3601 tccgccagcg aagcggtaat gaaggcagtt tacccagcca gcctttacgc accaccaggc
     3661 ctttatgaat acgcttactg gtacgtccgg agttcaacgc tttgtacagc ggctccaccg
     3721 ccgcaatctc ttcatccgtc gcccgggtac gaatcgacat atagccactg atttttccct
     3781 cgcgcaccat cggtaccgca ttggcccgca cccaataatg gtcgccattt ttgcggcgat
     3841 ttttcacgat gccgctccag ggctcccctt ttttcagggt gaaccacata tccgcaaacg
     3901 ccgcttttgg catatccggg tgacgcacca tgttgtgcgg ctgcccttgt aactcttgca
     3961 aggtaaagcc gctcacctgc acaaaagtgt cattagcatg agtgatatag ctttgcagat
     4021 cggtagtgga catcagagtg gtatcgtccg ccagcggggt attttgctgg gtgacatacg
     4081 gatgagaaga catgttcgcg tcctgtgcag gttatatggt tgttaacttc ttgtcagagt
     4141 ttatgtcggc cccgctgcgg ttatctttaa ccgattaatt tgatttagat cgcaatttgc
     4201 gattaaagca caaatctaat tctttgattt aaaatacttt cactctgtta ctatacgaaa
     4261 acgttaatta tcttgcccaa aaatcaggca attattgccc tgaaaacgtg catttgcgca
     4321 gcaatcatca aatccatacc cgacaaaaac cgtgcaaaat aacaacaaat gttaacggat
     4381 agcattaaat attgcacaaa tgataaccgc atttgtgttt atcccgattt tcgcgatcgc
     4441 agccggagtg gcgcaatccc tgcaatactt aaatcggtat catgtgatac gcgagcctcc
     4501 ggagcatatt ttgaacaggt taccttcgag cgcatcggct ttagcgtgca gcgcccacgc
     4561 cctgaatctc attgagaagc gaacgctgga tcatgaggag atgaaagcac ttaaccgaga
     4621 ggtgattgaa tacttcaaag agcatgtcaa tccggggttt ttagagtatc gcaaatctgt
     4681 taccgccggc ggggattacg gagccgtaga gtggcaagcg ggaggtttaa atacgcttgt
     4741 cgacacccag ggacaggagt ttatcgactg cctgggaggt tttggaattt tcaacgtggg
     4801 gcaccgtaat ccagttgtgg tttccgccgt acagaatcaa cttgcgaaac aaccactgca
     4861 cagccaggag ctactcgatc cgttacgggc tatgctggcg aaaacccttg ctgcgctaac
     4921 gcccggtaaa ctgaaataca gcttcttctg taatagcggc accgagtccg ttgaagcggc
     4981 gctgaagctg gcgaaggctt atcagtcgcc gcgcggcaaa tttactttta ttgccaccag
     5041 cggcgtgttc cacggtaaat cacttggcgc gctgtcggcc acggcgaaat cgaccttccg
     5101 caaaccgttt atgccactgc tgccagggtt ccgtcatgtg ccgtttggca atatcgaagc
     5161 catgcgcacg gctcttaatg agtgcaaaaa aaccggtgat gatgtggctg cggtgatcct
     5221 cgaaccgatt cagggcgaag gtggcgtaat tctgccgccg ccgggctatc tcaccgccgt
     5281 gcgtaagctg tgcgatgagt tcggcgcact gatgatcctc gatgaagtac aaacgggcat
     5341 ggggcgcacg ggcaagatgt ttgcctgcga acatgagaac gttcagccgg acattctgtg
     5401 tctggcaaaa gccctcggcg gcggcgtgat gccgattggc gcgaccatcg ccactgaaga
     5461 ggtgttttca gttctgttcg acaacccatt cctgcatacc accacctttg gcggcaaccc
     5521 gctggcctgt gcggcggcgc tggcgaccat caatgtgttg ctggagcaga acttaccggc
     5581 tcaggctgag caaaaaggcg atatgttgct ggacggtttc cgtcaactgg cgcgggaata
     5641 tcccgatctg gtacaggaag cgcgtggtaa agggatgttg atggcgattg agtttgttga
     5701 taacgaaatc ggctataact ttgccagcga gatgttccgc cagcgcgtac tggtggccgg
     5761 aacgctcaat aacgccaaaa cgatccgcat tgaaccgcca ctgacactga ccattgaaca
     5821 gtgtgaactg gtgatcaaag cggcgcgtaa ggcgctggcg gccatgcgag taagtgtcga
     5881 agaagcgtaa tatcaaatcg gatggcgata cgacgtcgcc atccgatctt tttatacgac
     5941 gcgtacgccc gcaggcatca tccgctccgg cgttaacaac acgctttcac tgccgtcatc
     6001 ggtttccgca cacaacagca tgcattccga cgtttcacca cgcattttcg ctttttgcag
     6061 attgcataag accaccaccg ttttccccat cagctcctct tcgctgtagt acggcaccag
     6121 gctggtcacg gtttgcagtg ttttttcccc acatcaacct gtacgatgta cagcttgtcg
     6181 gcgttttcat ggcgtttcac ttccacaatc tttccgacgc gcatttccag acgtgcaaaa
     6241 tcagcgtaag ccacggtttc cattgcttcc tcccttcgag taaaatttta ctaaactata
     6301 gaaaagtttt tctcaatcct gtaggctaaa aatggagaat gcaggcgtga tcacattcct
     6361 aagccgctgt gttaccgtta cagcgtcaaa gaaacgcgct ttatttactg aaaacaggtg
     6421 acccgataag cacttcctct acaatggggg cgcacatcag ggaaagtaaa aaaggtaaac
     6481 atggcaacat taaaagacat cgcaatcgaa gctggcgtat ccctggcgac agtatccagg
     6541 gtcttaaatg acgatccgac attgaatgtg aaagaagaga cgaaacatcg cattctcgag
     6601 atcgccgaaa agctggagta caagaccagt agcgcccgta aactacagac aggcgcagtc
     6661 aaccaacacc atattctggc tatctacagc taccagcagg agctggagat caacgatcct
     6721 tactatctgg cgatccgcca cggcattgaa actcagtgcg aaaagctggc gatcgaactg
     6781 accaactgtt atgaacacaa cggattacca gacattaaaa acgtcaccgg tattttaatt
     6841 gtcggcaaac ccacgcccgc cctgcgcgcc gctgccagcg cgttgaccga caatatctgt
     6901 tttatcgact ttcacgaacc cggcagcggt tacgatgcgg tggatatcga tctggcacgc
     6961 atcagtaaag aaatcatcga cttctatatc aaccagggcg ttaatcgtat tggttttatt
     7021 ggcggtgaag atgagcctgg caaggcggat attcgtgagg tcgcctttgc ggaatatggc
     7081 cgactgaaac aagtggtacg cgaagaggat atctggcgcg gcggtttttc cagttcgtcg
     7141 ggttatgaac tggcaaaaca aatgctggcg cgggaagact atccgaaggc actgtttgtt
     7201 gcttccgatt ccattgctat cggcgtactg cgggcaattc atgaacgtgg cctgaacatc
     7261 ccacaagata tttcgcttat cagcgttaac gatatcccca ccgcgcgatt tacctttccg
     7321 ccgctctcca ccgtgcgcat ccattccgag atgatgggaa gtcagggcgt taacctggtg
     7381 tatgaaaaag cccgcgatgg tcgcgcgctg ccgctgctag tcttcgttcc cagcaaatta
     7441 aaactgcgcg gcacgacccg ttaaatcccc ttacacactg ttcggcaatc atttttgccg
     7501 gacagtgctg ccgtttattt tcgtgatcca gttaaagtaa atgcatttac ctgctgcttt
     7561 ttagtaaaaa ttttactaaa cccccagcaa ttacacaaac taccatcacc atgaatggtt
     7621 ccgatttctc tctaccggga ggccctatga atcgctggga aaacattcag ctcacccacg
     7681 aaaaccgact tgcgccgcgt gcgtactttt tttcatatga ttctgttgcg caagcgcgta
     7741 cctttgcccg cgaaaccagc agcctgtttc tgcccttaag cagtcagtgg aatttccact
     7801 tttttgacca tccgctgcaa gtaccagaag ccttcacctc tgagttaatg gctgactggg
     7861 ggcatattac cgtccccgcc atgtggcaaa tggaaggtca cggcaaactg caatataccg
     7921 acgaaggttt tccgttcccc atcgatgtgc cgtttgtccc cagcgataac ccaaccggtg
     7981 cctatcaacg tattttcacc ctcagcgacg gctggcaggg taaacagacg ctgattaaat
     8041 ttgacggcgt cgaaacctat tttgaagtct acgttaacgg tcagtatgtg ggtttcagca
     8101 agggcagtcg cctgaccgca gagtttgaca tcagcgcgat ggttaaaacc ggcgacaacc
     8161 tgttgtgtgt gcgcgtgatg cagtgggcgg actctaccta cgtggaagac caggatatgt
     8221 ggtggtcggc ggggatcttc cgcgatgttt atctggtcgg aaaacaccta acgcatatta
     8281 acgatttcac tgtgcgtacc gactttgacg aagcctattg cgatgccacg ctttcctgcg
     8341 aagtggtgct ggaaaatctc gccgcctccc ctgtcgtcac gacgctggaa tataccctgt
     8401 ttgatggcga acgcgtggtg cacagcagcg ccattgatca tttggcaatt gaaaaactga
     8461 ccagcgccag ctttgctttt actgtcgaac agccgcagca atggtcagca gaatcccctt
     8521 atctttacca tctggtcatg acgctgaaag acgccaacgg caacgttctg gaagtggtgc
     8581 cacaacgcgt tggcttccgt gatatcaaag tgcgcgacgg tctgttctgg atcaataacc
     8641 gttatgtgat gctgcacggc gtcaaccgtc acgacaacga tcatcgcaaa ggccgcgccg
     8701 ttggaatgga tcgcgtcgag aaagatctcc agttgatgaa gcagcacaat atcaactccg
     8761 tgcgtaccgc tcactacccg aacgatccgc gtttttacga actgtgtgat atctacggcc
     8821 tgtttgtgat ggcggaaacc gacgtcgaat cgcacggctt tgctaatgtc ggcgatatca
     8881 gccgtattac cgacgatccg cagtgggaaa aagtctacgt cgagcgcatt gttcgccata
     8941 tccacgcaca gaaaaaccat ccgtcgatca tcatctggtc gctgggcaat gaatccggct
     9001 atggctgtaa catccgcgcg atgtaccatg cggcgaaagc gctggatgac acgcgactgg
     9061 ttcattacga agaagatcgc gatgctgaag tggtcgatat tatttccacc atgtacaccc
     9121 gcgtgccgct gatgaatgag tttggtgaat acccgcatcc gaagccgcgc atcatctgtg
     9181 aatatgctca tgcgatgggg aacggaccgg gcgggctgac ggagtaccag aacgtcttct
     9241 ataagcacga ttgcattcag ggtcattatg tctgggagtg gtgcgaccac gggatccagg
     9301 cacaggacga ccacggcaat gtctggtata aattcggcgg cgactacggc gactatccca
     9361 acaactataa cttctgtctt gatggtttga tctattccga tcagacgccg ggatcgggcc
     9421 tgaaagagta caaacaggtt atcgcgccgg taaaaatcca cgcgctgact cgcggcgagt
     9481 tgaaagtcga aaataaactg tggtttacca cgcttgatga ctacaccctg cacgcagagg
     9541 tgcgcgccga aggtgaaacg ctcgcgacgc agcagattaa actgcgcgac gttgcgccga
     9601 acagcgaagc ccccttgcag atcacgctgc cgcagctgga cgcccgcgaa gcgttcctca
     9661 acattacggt gaccaaagat tcccgcaccc gctacagcga agccgggcat tctatcgcca
     9721 cttatcagtt cccgctgaag gaaaacaccg cgcagccagt gcctttcgca ccaaataatg
     9781 cgcgtccgct gacgctggaa gacgatcgtt tgagctgcac cgttcgcggc tacaacttcg
     9841 cgatcacctt ctcaaaaatg agtggcaaac cgacatcctg gcaggtaaat ggcgagtcgc
     9901 tgctgacccg cgagccaaag atcaacttct tcaagccgat gatcgacaac cacaagcagg
     9961 agtacgaagg gttgtggcag ccgaatcatt tgcagatcat gcaggaacat ctgcgcgact
    10021 ttgccgtaga acagagcgat ggtgaagtgt tgatcatcag ccgcacagtt attgcaccgc
    10081 cggtgtttga cttcgggatg cgctgtacct acatctggcg catcgctgcc gatggccagg
    10141 ttaacgtggc gctttccggc gagcgttacg gcgactatcc gcacatcatt ccgtgcatcg
    10201 gtttcaccat ggggattaac ggcgaatacg atcaggtggc gtattacggt cgtggaccgg
    10261 gcgaaaacta cgccgacagc cagcaggcta acatcatcga tatctggcgc agcaccgtcg
    10321 atgccatgtt cgagaactat cccttcccgc agaacaacgg caaccgtcag catgtccgct
    10381 ggacggcact gactaaccgc cacggcaacg gtctgctggt ggttccgcag cgcccaatta
    10441 acttcagcgc ctggcactat acccaggaaa acatccacgc tgcccagcac tgtaacgagc
    10501 tgcagcgcag tgatgacatc accctgaacc tcgaccacca gctacttggc ctcggctcca
    10561 actcctgggg cagcgaggtg ctggactcct ggcgcgtctg gttccgtgac ttcagctacg
    10621 gctttacgtt gctgccggtt tctggcggag aagctaccgc gcaaagcctg gcgtcgtatg
    10681 agttcggcgc agggttcttt tccacgaatt tgcacagcga gaataagcaa tgaggatcat
    10741 cgataactta gaacagttcc gccagattta cgcctctggc aagaagtggc aacgctgcgt
    10801 tgaagcgatt gaaaatatcg acaacattca gcctggcgtc gcccactcca tcggtgattc
    10861 attgacctac cgtgtggaaa atgactccgc gaccgatgcg ctatttaccg ggcatcgacg
    10921 ctattttgaa gtgcattact acctgcaagg gcagcaaaaa attgaatatg cgccgaaaga
    10981 gacattacag gtagtggaat attatcgtga tgaaactgac cgtgaatatt taaaaggctg
    11041 cggagaaacc gtagaggtcc acgaagggca aatcgttatt tgcgatatcc atgaagcgta
    11101 tcggtttatc tgcaataacg cggtcaaaaa agtggttctc aaagtcacca tcgaagatgg
    11161 ttatttccat aacaaataac aactacggcg gcaaaaggag tttgccgcca ccgctaccct
    11221 acacattttc ggagatgtgt tatgtctgat accaaacgta atacaatcgg caaattcggc
    11281 ttgctctcgc tgacttttgc cgccgtttac agctttaaca acgttatcaa taataatatt
    11341 gagcttggac tggcctcggc accgatgttt ttcctcgcga cgatttttta ttttattccc
    11401 ttctgtctga tcatcgcaga atttgtttcg ttaaataaaa actcagaagc cggtgtctac
    11461 gcgtgggtaa aaagttcgct gggcggacgt tgggcattta ttactgccta cacttactgg
    11521 ttcgtaaatc tgttcttttt cacctcgctg ttgccgcgcg ttattgctta tgcttcgtat
    11581 gccttcctcg gctacgaata tattatgacg ccggttgcca ccaccgttat cagtatggtg
    11641 ctgttcgcct tctccacctg ggtttccacc aacggggcga aaatgctggg gccaattacc
    11701 tccgtcactt caacgctgat gctgctgtta acgctctcct acattttact ggcaggtacg
    11761 gcactggttg gcggcgtaca gcctgccgat ccgatcaccg ttgacgcgat gatcccgaac
    11821 ttcaactggg cgttcctcgg cgttaccacc tggatcttta tggccgcagg tggcgcggag
    11881 tccgtcgcgg tgtacgttaa cgacgtcaaa ggcggttcga aatcgttcgt taaagtgatc
    11941 atcctcgccg ggatttttat cggcgtactg tattccgtct cctcggtgct gattaacgtc
    12001 ttcgtcagca gcaaagagtt gaaatttacc ggcggatcgg tgcaggtatt ccacggcatg
    12061 gcggcgtatt ttggtctgcc ggaagcgttg atgaatcgct ttgtcggtct ggtgtccttt
    12121 accgcaatgt tcggttccct gctgatgtgg accgcaacgc cggtgaaaat tttcttctcc
    12181 gaaatcccgg aaggcatctt tggtaagaaa accgtcgaac ttaacgaaaa cggcgttccg
    12241 gcgcgcgcag catggatcca gtttctgatc gtcatcccgc tgatgattat cccgatgctc
    12301 ggttccaata ccgtgcagga tctgatgaat accattatta atatgaccgc cgcagcgtcc
    12361 atgcttccgc cgttattcat catgctggct tacctgaatt tacgcgccaa attagatcac
    12421 ctgccacgcg atttccgtat gggctcccgc cgcaccggta ttatcgttgt ttcaatgctg
    12481 attgcgatat ttgccgtagg gtttgtcgct tcgacattcc cgactggcgc gaatattctg
    12541 accatcattt tttataacgt cggcggtatt gttatcttcc tcggctttgc gtggtggaaa
    12601 tacagcaaat atataaaggg attaacggct gaagagcgcc atattgaagc gacgccagcc
    12661 agcaatgttg attaagcaga ctaataaaaa atgagcaggc attttccctc ttcctggatg
    12721 aggagaggga aaatagtttc tgcctttata tttttataat tacaacgata aaaggctgta
    12781 ctttttcttt agctcatgga ttaacacaat gaaattaatc actgcaccat gcagagcatt
    12841 acttgctctg ccgttttgct acgccttttc tgcggcagga gaagaagcac gtccggcaga
    12901 acatgacgac acaaaaacac ccgcaattac ctcgacatct tctccttcat ttcgttttta
    12961 cggcgaatta gggcttggtg gatatatgga tttagagggt gagaataaac ataaatacag
    13021 cgacggcacc tatattgaag gtggcctgga gatgaagtac ggctcctggt tcggcctgat
    13081 ttacggcgaa ggctggaccg tgcaggccga ccacgacggt aatgcctagg tgcctgacca
    13141 tagctggggt ggtttcgaag gtgggattaa ccgtttctat ggcggttatc gtaccaatga
    13201 tggcaccgaa atcatgctca gtctgcgtca ggattcctcg ctggatgacc tgcaatggtg
    13261 gggcgacttc acacccgatt tgggctacgt cattcccaat acccgcgaca ttatgaccgc
    13321 gctgaaggta cagaacttaa gcggcaactt tcgttatagc gtcaccgcca cacctgccgg
    13381 acatcatgac gaaagcaaag cctggctaca ttttggcaag tacgatcgct acgacgacaa
    13441 atacacctat ccggcaatgg atgaacggtt acatccagta tgaccttgcc gaaggcatca
    13501 cctggatgaa tggtctggaa attaccgacg gcacaggaca actctatctc acgggcctgt
    13561 ttacccctaa ctttgccgct cgcgcctggc accataccgg acgtgccgac gggctggacg
    13621 taccgggaag tgaaagcggg atgatggtga gcgccatgta tgaagcgtta aaaggcgttt
    13681 atctctccac cgcttacacc tacgccaaac atcgccctga ccacgctgac gatgaaacca
    13741 cctctttcat gcagtttggt atctggtacg aatatggcgg cggacgtttc gccacggctt
    13801 ttgatagccg cttctacatg aaaaatgcct ctaacgatcc cagcgaccaa atcttcctga
    13861 tgcaatattt ctactggtaa taaggactgt aatctcatga aaatcaaaac tattttaacg
    13921 ccagtaacct gcgctctgct gataagtttt tccgctcatg ccgctaacgc cgacaattat
    13981 aaaaacgtga ttaaccgtac tggtgcgccg cagtacatga aggattacga ttacgacgat
    14041 caccagcgtt ttaatccgtt tttcgatctc ggagcctggc atggtcatct gttgccagac
    14101 ggcccgaaca ccatgggcgg ctttccgggc gttgcgctgc tgacggaaga atacatcaac
    14161 tttatggcca gcaatttcga ccgcctgact gtctggcaag acggcaagaa agtcaacttc
    14221 acgctggagg catacagtat tcccggcgcg ctggtgcaaa aactgacagc aaaagatgtg
    14281 caggtcgaaa tgactctgcg cttcgccacg ccgcgcacgt cactactgga aactaaaatc
    14341 atcagcaata aaccgctgga tctggtgtgg gatggcgaac tgctggaaaa actggaagcg
    14401 aaagaaggga aaccgctttc cgataaaacc attgctggcg aataccctga ctatcagcgc
    14461 aaaatcagcg ccacccgtga tggcctgaaa gtcacctttg gcaaagtgcg cgccacctgg
    14521 gatctgctga cctccggcga atcggaatat caggtgcata aatccctgcc ggtgcagact
    14581 gaaatcaacg gtaatcgctt taccagtaag gcgcatatca acggttcgac cacgctctac
    14641 accacctatt cccatctgct gaccgctcag gaagttagca aagagcaaat gcagatccgc
    14701 gatattctgg cgcgtccggc gttttatctc accgcctcgc agcaacgctg ggaagaatac
    14761 ctgaagaaag ggttaaccaa tccggatgcg acgcaggaac agacgcgcgt cgcggtgaaa
    14821 gcgatcgaaa cgctcaacgg taactggcgc tcaccgggcg gtgcggtgaa ttataacacc
    14881 gtcacgccgt cggtgaccgg acgctggttc tccggcaatc agacctggcc gtgggatacc
    14941 tggaagcagg cgtttgcgat ggcgcatttc aatccggata tcgccaaaga gaatatccgc
    15001 gcggtcttct cctggcagat ccagcctggc gacagcgtgc gtccgcagga tgtgggcttt
    15061 gtccccgacc tgatagcgtg gaaccttagc cccgagcgtg gtggcgatgg cggcaactgg
    15121 aacgaacgta ataccaaacc cagccttgcc gcctggtcgg tgatggaagt atacaacgtc
    15181 acccaggata aagcctggtt ggcagagatg tacccgaaac tggtggccta tcacgactgg
    15241 tggttacgta accgcgatca taacggcaac ggggtgccgg aatatggcgc gacccgcgac
    15301 aaagcccaca acaccgagag cggcgagatg ctgtttacgg tgaaaaaagg cgataaagaa
    15361 gagacgcagt ctgggctgaa caactacgcc cgcgtggtgg agaaaggcca gtacgacagt
    15421 ctggaaattc cggcacaggt cgctgcgtcg tgggaatcgg ggcgtgatga tgccgccgtc
    15481 tttggtttta tcgacaaaga acagttggat aaatatgtcg ctaatggcgg caaacgtagc
    15541 gactggacgg tgaaattcgc cgaaaaccgc agtcaggacg gaacgttgct gggctactcg
    15601 ctattgcagg agtcggtgga tcaggccagc tatatgtaca gcgataacca ttatctggcg
    15661 gagatggcaa ccatcctcgg taagccagaa gaggccaaac gctatcgcca gttggcacag
    15721 cagctcgcgg actacatcaa cacctgtatg ttcgacccga ctacccagtt ctactatgac
    15781 gtacgtattg aagataaacc actggcgaac ggctgcgcgg gcaaaccgat tgttgagcgc
    15841 ggtaaagggc cggaaggctg gtcgccgctg tttaacggtg cggcaacgca ggccaatgcc
    15901 gacgcggtgg tgaaggtgat gctcgatcct aaagagttca acacatttgt tccgctggga
    15961 acggcggcgt taaccaatcc ggcttttggc gctgatatct actggcgcgg gcgcgtatgg
    16021 gtggatcagt tctggtttgg tctgaaaggg atggagcgtt acggttatcg tgatgatgcc
    16081 ctgaagctgg cggatacgtt cttcaggcac gccaaaggat taaccgccga tggcccaatt
    16141 caggagaatt acaacccgct gacaggcgcg cagcaaggcg caccaaattt ctcctggagt
    16201 gccgctcatt tgtatatgtt gtataacgat tttttccgta agcagtaatt tgcaacggct
    16261 ggcggatgcg gcgtgaacgc cttatccgcc ctacatgtgc aatcccccaa gccggataaa
    16321 acacggcaag cgtcgcatcc ggcgttacca ccgggcgtat tctttttgaa tcccatcaca
    16381 aaccccgcac tccccttttc ccttttctcc atcgacggct aaattagaac tcatccgact
    16441 acataacaat tattttacat actggacaat tttatgagct acccgtcgct gttcgccccg
    16501 ctggatttag gttttaccac gttaaaaaac cgcgtgttga tgggttcaat gcacaccggg
    16561 ctggaggaat acccggacgg tgctgaacgg ctggctgcgt tttatgccga acgcgcccgt
    16621 cacggcgtgg cgctgattgt cagcggcggc attgcaccag atttaacagg cgttggcatg
    16681 gaaggtggcg caatgctcaa cgacgccagc cagatcccac accatcgcac cattaccgaa
    16741 gccgtacatc aggaaggcgg caaaatcgcc ctgcaaattt tgcataccgg gcgctacagc
    16801 taccaaccgc atctggtcgc accgtccgca ttgcaggccc ccatcaaccg tttcgttcct
    16861 cacgaactca cccatgaaga gatcctgcaa ctgatcgacg atttcgcccg ctgcgcgcaa
    16921 ctggcgcggg aggccggata cgatggcgtc gaagtgatgg gctctgaagg gtatttgatc
    16981 aacgaatttc tgacgctgcg caccaatcag cgtagtgacc agtggggcgg cgattaccgc
    17041 aaccggatgc gatttgccgt agaagtagtg cgtacggtgc gcgaacgcgt cggcaacgac
    17101 ttcattatta tttaccggtt gtcgatgctc gacctagtcg aaggcggcgg cacttttgcc
    17161 gaaacggtag aactggcgca ggccattgaa gcggcgggcg cgaccattat caacaccgga
    17221 ataggctggc acgaagcgcg gattccgacc atcgccacgc ccgtgccgcg cggcgcattt
    17281 agctgggtca cgcgcaaact gaaaggccac gtctcgctgc cgctggtaac caccaaccgg
    17341 attaacgatc cgcaggttgc cgacgatatt ctctcgcgcg gcgatgccga tatagtatcg
    17401 atggcgcgac cgtttctcgc ggatgcggaa ctgctgtcaa aagcgcaatc agggcgagcc
    17461 gatgagatca acacttgtat tggctgcaac cagggctgcc tcgatcagat cttcgttggc
    17521 aaagtcacct cgtgcctggt gaatcctcgc gcctgccacg aaaccaaaat gccaatcctt
    17581 cccgccgtgc agaaaaaaaa tctggcggtg gtcggcgctg gacctgctgg gctggcgttt
    17641 gccattaacg cggcggcgcg tgggcatcag gtaacattgt ttgatgcgca cagcgagatc
    17701 ggcgggcagt ttaatatcgc caaacagatc cccggcaaag aggagtttta cgaaacgctg
    17761 cgctactacc gccggatgat cgaagtgacg ggcgtgacgc tgaaactcaa tcacaccgtg
    17821 acggcggatc agttacaggc gttcgatgaa acgatcctcg ccagtgggat cgtgccgcgt
    17881 gttccgccta tcgacgggat cgatcatccg aaggtattga gttatctcga tgtactgcgc
    17941 gacaaagcgc cggtaggcaa caaggttgcc atcatcggtt gtggcgggat tggttttgat
    18001 acggcgatgt atttaagtca gccgggcgaa tccaccagcc agaatatcgc caggttctgt
    18061 aatgagtggg ggatcgacag cagcctacaa caggctggtg gcttaagccc gcagggaatg
    18121 cagatcccgc gtagcccacg gcaaattgtg atgctccagc gcaaagccag caaaccggga
    18181 caggggttag gcaaaaccac cggctggatc catcgcacca ccctgctctc gcggggtgtg
    18241 aaaatgatcc caggcgtaag ttatcagaag attgacgatg acgggctgca tgtggtgatc
    18301 aacggcgaaa cgcaggtatt agcagtggac aatgtggtga tctgcgcagg gcaagagcca
    18361 aaccgcgcgc tggcgcaacc gctgattgat agcgggaaaa ctgtgcattt aattggcggc
    18421 tgcgatgtgg ctatggagct ggacgcacga cgggcaattg cccagggaac acggctggcg
    18481 ctggagattt aaatcgttat ttgaagcgcc ggatgcaacg cattcggcac gtcgcgttaa
    18541 tcaataaaca aggcgggaga aatgccgaat cgcgttgcca attttttagc gtgttccagc
    18601 gttaatttcc tcttcccgct caaaacgcgt gacaccatag atttactgcc aatttccggc
    18661 agatcggaaa gggttaaacc atattgatcc ataagggtac gaatcacggc tataccgcca
    18721 ggcatggctt gagccatggc attaaattcc gcaaattcgg gcgctgattc ttcccacgcg
    18781 gttattttgg cacacaccag atccagcaag gggttttcag gatcgttgag cagcagatga
    18841 tctaccagtt ccagcgcctg ggtgtattgt tcctcgttct gaatacccgc cagaaaaggt
    18901 gccacagcag ttagcttttc tcctgcttgc aagatgtcgg caatcgcaat cattttttcc
    18961 ccttagtacg atgaacagcg gtaaagaaat cgtattcttt atgcgtcata acttcacgta
    19021 tgtagcactt ttgcgattca aaaaagacca ttgctacaac acgtaattca ttgcccccaa
    19081 cattgaaaac ataatgctta tccagatatt tgaagttatc cagagatggg aatactgctt
    19141 ttaatgactc aggttttttg aaatatccct tagcaatcgt gttccccaga gccaccaact
    19201 ccgttttatg ttgcgggtat ttttccgcag catctttcaa tgctttttga gttatcaggt
    19261 gcattcttca tcacgtccgt tgacaaattg gcaatatgat aacatccgtt gacagattgg
    19321 caacggatga atttatctgt gtgacggaag aataaacgta gaaaaacgct aaacaagaga
    19381 atgtagagga tggtgacagg gattttttgt tttatggaag cgcgattcca gaactgagca
    19441 gacattgccg gatgcgacgc ttgccgcgtc ttatccggcc tgggggtaac gtgctgaacg
    19501 taggccggat aaggcgttta cgccgcatcc ggcaggtaac tcccaatctt accgacgacg
    19561 tcccagcttc accgctttca gcaccacaaa tttattattc gtcgcaatag tggtgcagtt
    19621 gccgaaaatt ttcttcagtt tatggaagta atccagatgg cggttggcaa cgatatacag
    19681 ctcgccgttg attttcaggc agcggcgggc gtagtggaac atctcccagg cgacgttatc
    19741 ggtcagcgca tgttgttggt gaaacggagg gttgcagagc acagcattaa agcggaaagg
    19801 ctccacgccg gagagcgcgt tgttgatcat aaactcgctg cgatccaacg cctctggcat
    19861 gttggtttca acgttcaaac ggctggaagc aaccgccatc ggcgattcat cgacaaacac
    19921 cactttcgcc tgcgggtttt tatcaagcag cgtcagacca ataacgccat taccgcaacc
    19981 gagatcgaca atctcccctt cgagattctc tggcagatgt tgcataaaga agcgcgcgcc
    20041 gatatcaagc ccggtgcggg agaagacatt cgcatggtta tggatagtcc agtcagtacc
    20101 ttccagcttc cagctaacgg tctgcggcgc atcggccagc ggcggttcat tgaacgtgca
    20161 attaatcagg cgcgcttttt tccatgccag cgtggtggtg gtcgggccga gcactttttc
    20221 gaacagttcc agcgtggaag tgtgaatgtc acgggctttg gcaccggcaa taatacgtgt
    20281 atccgacgtg accactttgc gcagcgcacg cagttgctgt tccagcaatg ccagtgtttt
    20341 cggcactttg atcagcacca cacccggctg ttgcgggtag tcggcggtgc tgtcgagaaa
    20401 cttcacgctc gactcatcaa tcccgtttag gcgtaaattc tcgcgcgtcg ccagttcact
    20461 gatgtatgag tcgccaatgc tgtacggctt atgttccgcc agcgcgcagc ttaacgcacc
    20521 aaaggcatca ttcaggatca acaccgggcc gaggatttct gtgtcgtcca actgttgcag
    20581 caaatattca tctgccgctt cccacgcctg tagcgggtta acgtcatccg tcgccggaaa
    20641 acgttgtagt gtcagtgaac ggaaaccgtt gtctaagtgg ctcatcggcc ctcctgaatg
    20701 ataaaatttc gacgttgccc ctgaaaaggg tgcgtgagta tacccttttt ctctttttcg
    20761 tgggcggtta tgagcaatct tacttatctt cagggttatc ccgagcagct gctttcgcag
    20821 gtgcgaacac tgattaacga acagcgtctg ggcgatgtgc tggcaaaacg ctatccggga
    20881 acacacgact acgccaccga taaagctctc tggcaatata ctcaggatct gaaaaatcag
    20941 tttctgcgta atgccccgcc gatcaataaa gtgatgtatg acaataagat ccatgtgcta
    21001 aaaaacgcgc tcgggttaca taccgctgtt tctcgtgtgc agggcagcaa gctgaaagca
    21061 aaagcagaga tccgcgtcgc taccgtgttt cgcaacgcgc cggaaccgtt tttgcgcatg
    21121 atcgtggtgc acgagctggc gcacctgaag gagaaagagc ataacaaagc gttctatcaa
    21181 ttgtgctgcc atatggagcc gcagtaccac cagcttgagt tcgacacccg gctgtggcta
    21241 acgcagttgt cgcttgggca ggacaaaatc tgaaaatgat gtcaggcgtt atcttttctt
    21301 tctttagcgc ctaaaatcga cctcccccct ttcgttaagg ataacgacca tgctgcgcgc
    21361 atttgcccgc cttcttctcc gtatttgttt ttctcgccgg actctaaaga ttgcctgctt
    21421 actgttactt gtggcaggag cgaccatttt tatcgccgat cgcgttatgg tcaacgccag
    21481 caagcagctg acctggggcg atgtcaacgc cgtcccggcg cgtaacgtgg gtttattgct
    21541 gggggcgagg ccgggtaatc gctactttac ccgacgtatt gataccgcag cagcgttgta
    21601 ccacgccgga aaagtgaaat ggctactggt gagtggcgat aacggacgta aaaattacga
    21661 tgaagcatca ggtatgcagc aggctttgat cgctaaaggc gtaccggcga aagtgatctt
    21721 ttgcgactat gccggattct caacgctgga ttcggtagtg cgtgccaaaa aggtgtttgg
    21781 ggaaaaccat atcaccatta tctcgcaaga atttcataac caacgcacca tctggctggc
    21841 aaaacagtac ggcatcgacg ctatcgggtt taacgcgcca gacctcaata tgaagcacgg
    21901 tttttatact caactgcggg agaaactcgc tcgtgtcagc gcggtgattg acgcaaaaat
    21961 cctccatcgc cagccgaaat atctcgggcc atcggtcatg attgggccgt ttagtgagca
    22021 tggctgtccg gcgaaagaat aatgcgtatc tgcgcacgtc gaagatgaaa aaggcgtgct
    22081 acattgacga cagaatccct ttatggagta tccacgcgtt atgatacgtt tcgctgtgat
    22141 tggtacgaac tggatcactc gccagttcgt cgaggccgcc catgagagcg gtaaatacaa
    22201 gttaaccgcc gtatattccc gcagccttga gcaggctcag cacttcgcca atgatttttc
    22261 tgtcgagcat ctgtttacgt cgctggaagc gatggcggaa agcgatgcca ttgacgcggt
    22321 gtatattgcc agcccgaatt ccctgcactt ttcccagaca caacttttcc ttagccataa
    22381 aattcatgtg atttgcgaaa aaccgctggc gtcgaatctg gcggaagtgg atgccgccat
    22441 tgcctgtgcg cgcgaaaatc aggtggtgct gtttgaggca tttaaaaccg cctgcctgcc
    22501 gaactttcat ttgttgcgcc aggcgctgcc gaaagcaggc aaactgcgta aagtcttttt
    22561 caactattgc cagtattcct cgcggtatca acgttacctg gacggtgaga atcccaacac
    22621 ctttaatccg gcattctcta acggttcaat tatggatatc ggcttttact gcctggcgtc
    22681 ggcggtggcg ttatttggtg agccgaaaag cgtgcaggca acggcgagtt tgctggcaag
    22741 cggcgttgac gcccacggcg tggtggtgat ggattacggt gatttcagcg tcaccttgca
    22801 gcactcaaag gtcagtgatt ctgtcctggg aagcgagatt cagggcgaag cgggatcgct
    22861 ggtgattgaa aaactgtctg aatgccagaa agtctgcttt gtgccgcgtg gcagccagat
    22921 gcaggatctc acccagccgc agcatattaa taccatgctc tacgaagcag agctgttcgc
    22981 taccctggtg gatgagcatc tggtggatca tccagggctg gcggtcagtc gcatcacagc
    23041 caaactgctg accgagatcc gccgccaaac tggggtgatt tttccggcag atagcgtaaa
    23101 actatacttg ccaaagtaaa acagtgtaaa aggtatgtaa cagaccattg actgactgaa
    23161 tggtctgtca tactttgtta cctgcaaagg ggagtaactt cattgccggt cgatcgtcat
    23221 tacgatgagt gaaaaaccac atccggtcac cgggcaaccc gaaaggaata cgcagacgta
    23281 ttcctttttt gttgtaagtg agaccttgcc ggaaggcgag gtctatgcat aaaaagcagc
    23341 ggctgacgtc ttccgacgtt ggccgttttt ttatgtgtaa ggaacttcta tgaatactgt
    23401 cggcacgccg ttgctatggg gcggattcgc tgttgttgtc gccattatgc tggctatcga
    23461 cctgttgttg caggggcgtc gtggggcaca tgccatgacc atgaaacagg ctgcggcctg
    23521 gtcgctggtc tgggtgacgc tgtcgttact gtttaacgcc gctttctggt ggtatctggt
    23581 gcaaaccgaa ggtcgcgccg ttgccgatcc acaggccctg gcctttctca cgggttatct
    23641 gattgagaaa tcgctggcgg tcgataacgt ctttgtctgg ctgatgttgt tcagctattt
    23701 ctccgttccg gcggcattac aacgtcgcgt gctggtgtat ggcgtgctcg gggcgattgt
    23761 tctgcgtacc atcatgatct tcacaggcag ctggctgatt tcgcagttcg actggatcct
    23821 gtatatcttc ggtgccttcc tgctgtttac cggcgtgaag atggcgctcg cccatgaaga
    23881 tgaatcaggc attggcgaca agccgctggt gcgctggcta cgcggtcatt tgcgcatgac
    23941 cgacaccatc gacaacgagc atttctttgt gcgtaagaat ggattgctgt acgccacacc
    24001 gctgatgctg gtactgattc tggtggaatt gagcgacgtg atttttgccg tggatagcat
    24061 tccggctatc ttcgccgtga ccactgaccc gttcattgtg ctgacctcaa acctgtttgc
    24121 aatcctcggc ctgcgtgcga tgtatttcct gctggcgggc gtagcagagc gtttctcgat
    24181 gctcaaatat ggcctggcgg tgattctggt gtttatcggt atcaagatgc tgattgtcga
    24241 cttctaccat attccaatcg ccgtctcgct gggcgtggtg tttggcattc tggtgatgac
    24301 gtttattatc aacgcctggg tgaattatcg gcatgataag cagcggggag aatgattcgc
    24361 tgtatattaa gccattccta gagataactc attccagaca aaggtgagct attaattatg
    24421 taatcggctt atagctcttt acaaaaagac cgcacgggtg ttggtggcgt caaaatcgcc
    24481 gaagcgttcc ctggaggccg gggcgagggc gcatcttaca gggacgttac cttgcgcccg
    24541 acccggtagc cgcaagggat aggctgaggg aacc
//
LOCUS       DAZPAB010000041        24382 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZPAB010000041 DAZPAB010000000
VERSION     DAZPAB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.041"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(171..1052)
                     /gene="prmA"
                     /locus_tag="ACHUPG_001488"
     CDS             complement(171..1052)
                     /gene="prmA"
                     /locus_tag="ACHUPG_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR0861153.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(1064..2515)
                     /gene="panF"
                     /locus_tag="ACHUPG_001489"
     CDS             complement(1064..2515)
                     /gene="panF"
                     /locus_tag="ACHUPG_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR0861154.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2505..2747)
                     /locus_tag="ACHUPG_001490"
     CDS             complement(2505..2747)
                     /locus_tag="ACHUPG_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR0861155.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2856..4205)
                     /gene="accC"
                     /locus_tag="ACHUPG_001491"
     CDS             complement(2856..4205)
                     /gene="accC"
                     /locus_tag="ACHUPG_001491"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR0861156.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4216..4686)
                     /gene="accB"
                     /locus_tag="ACHUPG_001492"
     CDS             complement(4216..4686)
                     /gene="accB"
                     /locus_tag="ACHUPG_001492"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR0861157.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAKSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5664..6638)
                     /locus_tag="ACHUPG_001493"
     CDS             complement(5664..6638)
                     /locus_tag="ACHUPG_001493"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR0861158.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            6790..8730
                     /gene="csrD"
                     /locus_tag="ACHUPG_001494"
     CDS             6790..8730
                     /gene="csrD"
                     /locus_tag="ACHUPG_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR0861159.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            9035..10078
                     /gene="mreB"
                     /locus_tag="ACHUPG_001495"
     CDS             9035..10078
                     /gene="mreB"
                     /locus_tag="ACHUPG_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR0861160.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            10144..11247
                     /gene="mreC"
                     /locus_tag="ACHUPG_001496"
     CDS             10144..11247
                     /gene="mreC"
                     /locus_tag="ACHUPG_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR0861161.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            11247..11735
                     /gene="mreD"
                     /locus_tag="ACHUPG_001497"
     CDS             11247..11735
                     /gene="mreD"
                     /locus_tag="ACHUPG_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR0861162.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            11744..12337
                     /gene="yhdE"
                     /locus_tag="ACHUPG_001498"
     CDS             11744..12337
                     /gene="yhdE"
                     /locus_tag="ACHUPG_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR0861163.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            12327..13796
                     /gene="rng"
                     /locus_tag="ACHUPG_001499"
     CDS             12327..13796
                     /gene="rng"
                     /locus_tag="ACHUPG_001499"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR0861164.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            13864..17664
                     /gene="yhdP"
                     /locus_tag="ACHUPG_001500"
     CDS             13864..17664
                     /gene="yhdP"
                     /locus_tag="ACHUPG_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR0861165.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            17820..19265
                     /gene="tldD"
                     /locus_tag="ACHUPG_001501"
     CDS             17820..19265
                     /gene="tldD"
                     /locus_tag="ACHUPG_001501"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR0861166.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(19393..20322)
                     /gene="aaeR"
                     /locus_tag="ACHUPG_001502"
     CDS             complement(19393..20322)
                     /gene="aaeR"
                     /locus_tag="ACHUPG_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFR0861167.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            20505..20708
                     /gene="aaeX"
                     /locus_tag="ACHUPG_001503"
     CDS             20505..20708
                     /gene="aaeX"
                     /locus_tag="ACHUPG_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR0861168.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            20716..21648
                     /gene="aaeA"
                     /locus_tag="ACHUPG_001504"
     CDS             20716..21648
                     /gene="aaeA"
                     /locus_tag="ACHUPG_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFR0861169.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            21654..23621
                     /gene="aaeB"
                     /locus_tag="ACHUPG_001505"
     CDS             21654..23621
                     /gene="aaeB"
                     /locus_tag="ACHUPG_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR0861170.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTHFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            23713..23985
                     /locus_tag="ACHUPG_001506"
     CDS             23713..23985
                     /locus_tag="ACHUPG_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR0861171.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(24041..24304)
                     /gene="yhcN"
                     /locus_tag="ACHUPG_001507"
     CDS             complement(24041..24304)
                     /gene="yhcN"
                     /locus_tag="ACHUPG_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR0861172.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         5792 a         6136 c         6605 g         5849 t
ORIGIN      
        1 tagaaaatag tgactaaaat ttacactcaa agaaatttga gtaagttctc aatttttctt
       61 tatgaatgaa aattttggca cgcaaatttg cgtaaataat cggcaaattt acgtgccttt
      121 tgtgagcttg ctcgcacttc gccccgcgtc accctacggc gatgcgaagg tcaattctta
      181 cgaccggtaa tacggcacca ctcttctttt tccacgaccg ggtccagtgc gaagctatcg
      241 gcataagctt cacaaacgct ctctgcctgg cttgccagaa taccggaaag gcccagcaaa
      301 ccgcctgaaa ctggcaggac gctgattaac ggtgccagtt cacgtaatgg gcctgcaagg
      361 atgttagcga ccaccacgtc ggctttcatt tcttctggct gatctttcgg taagtagagt
      421 tccagacggt cagaaacgcc attacgttcg gcgttatcgc ggctggcctg aatcgcctgc
      481 ggatcgatat caataccaat ggcttttgct gcacccagtt tcagcgccgc gatcgccaga
      541 atgccggaac cacagccaaa gtcgatgact gttttaccgg ttaaatcgag gctgtcgagc
      601 cattgcaggc acagagaggt ggttggatgg gtacccgtac caaacgccag ccctggatct
      661 aacatcacgt tgacggcgtt ttcgtccggc acatcacgcc agctagggca gatccacagt
      721 cgttcaccaa agcgcatcgg gtggaaatta tccatccatt cgcgctccca gtctttatct
      781 tctagttgtt cgattttatg cgcgaagcct gcgccgagca gcggatggtt ttccagaatc
      841 gccaccacgt cgttcatatc ggtttcagcg tcgaacagac caatcacatc ggtgtcgccc
      901 cacaggcgcg tttcgcccgg cagcggctca aatactggcg tatcgtgggt atcctgaaaa
      961 gtgatagaaa cggcacccgc ttccatcagc gcatcgctaa gatcttccgc gttcgcgccg
     1021 gtggtgttca gtttcagttg gatccaaggc atggcaaaac tctttattta tcagtagtca
     1081 aaacggtagc ttgcgggacg gatgtaccga aacggtttcc gaccaggaaa gccagcaaac
     1141 ttagtagtaa cgagggcacg atagggtgga agcccaggta ctgaatattc agcgtcgcga
     1201 gtacggcata cagcacgccg ccaacgatca tcgcacttag cgcgcctttg gcgttggcgc
     1261 gttcccagta aagacccagc accagcggcc acaggaaaac ggcttccagc ccaccgaagg
     1321 ccagcaaatt cagccagatg atcatttctg gcggcttcca ggcggcaagc agcagcaacg
     1381 cgccgagaac taacgtaatt accgccgaca tccgcttcag acgcgtctcg ttttgcattt
     1441 gatccggacg gatattcaga tagagatctt taatgatcgt agcggaactt tgcagcagtt
     1501 gggcgttaat tgtcgacatg atcgcagcca taggtgcagc cagaaagatc ccggcagcaa
     1561 aaggtggcag cacttttacc attaacgtcg ggatcaccag atctggtacg gtgagatcgg
     1621 ggatcactgc acgacctaac gctccggcca ggtgcatacc gaacatcagg attgcgacta
     1681 taatcgtacc gatgatgatc ccccggtgta ctgctttgct gtctttataa gagatacagc
     1741 gcaccgcagt atgcggcagg ccaatcacgc caaagcacac cagtacccag aacgacgtca
     1801 taaaggcagg cgacagaata tcgtcagcgc cttgtggcgt aaccagttgc ggatcgatgg
     1861 tttgcaaggt ctgtactgcg ttgcttaagc cgccagcggc atgtaccacg ccaataagca
     1921 gcacaacggt gccaatcagc atcacaagcc cttgcatggt gtcgttcagc acgctggcgc
     1981 gaaagccacc aaaggcggta tataacgcaa tgctgatacc aaaaatcagc agcccggttt
     2041 cataaggaat acccgccgcg gtttccagca ggcgcgcacc gccgataaac tgcacggtca
     2101 tcgcaccaac gaacgcaacc agcaaactca aactcgccag ccacaccaga agacgactct
     2161 ggtagcgggc aaacagcata tcgttcagcg tcactgcatt gtagcggcgc gcaagaatcg
     2221 caaacttctt gccgagaata ccgagtgaaa gccagacagc aggaagctga atcatcgcca
     2281 gcaataccca gcccagcccg tatttataag cagctcctgg cccgccgata aacgaactgg
     2341 cactgatata ggtcgcggtg agagtcatcg ccagcacaat accgcccata gagcggctgc
     2401 cgaggaaata ctcattaagg aaggtgccgg tgctccgttt acgcatcgca taaaccgaga
     2461 taccgaacac caccaccaga taggcgacca gcggtagaat tacttcaagc tgcatcgtca
     2521 tcctccagtg ggatatcgcg atagataaat ttcaccatcg cccagcacag tccaataaac
     2581 agtagcggcg tcaggatgca ggccatctca aaccagcgcg gaaagccggt aaaaccgggg
     2641 gcaacgccag gtaagtaagc gtctactaac caaactgcca gatacaaaag ggtcagcccc
     2701 agcgcccagc gggcctcttt atgggcctga acaaaacgag tgtccatttt ttgtccctga
     2761 tgggtgaaga aagcgaggat tgtaccttat gggggttgtc gatccccagt aataaaaaag
     2821 gccggaaaat ccggcctttt gacgcttttg cagtcttatt tttcctgaag accgagtttt
     2881 ttctccagat agtggatgtt agtgccacca tgctggaagt tctcgtcatt catgatgcgg
     2941 atctgcagat caacgttggt tttgataccg tcgatgatca gctcctgcag cgcattcttc
     3001 atgcgggcaa tcgccacgtc acggttttca ccgtagcaaa tcagcttacc gatcattgag
     3061 tcatagtacg gcggtacggt gtagcccgcg tagatatgag actcccaacg tacgccaaaa
     3121 ccgccaggtg cgtggaaacg ggtgattttg cctgggcttg gcaggaaggt gttcggatct
     3181 tcggcgttga tacgacattc caccgcatgg ccgcgaacgt gaacttcttc ttgcttgatc
     3241 gacagcggtt gaccggcagc gatacgcagc tgttctttga tcaggtcaac gccggtgatc
     3301 atttctgtaa ccgggtgttc tacctgaata cgggtgttca tttcgatgaa atagaactcg
     3361 ccgttttcga acaggaactc gaaagtacct gcaccgcgat agccgatatc aacacacgct
     3421 ttagcgcaac gttcgccgat gtagcgacgc agttccgggg taatgcccgg tgctggtgct
     3481 tcttcgacca ctttctggtg gcggcgctgc atggagcagt cacgttccgc cagatagata
     3541 gcgttgccct gaccgtcagc cagtacctga atctcgacgt ggcgaggatt ctccaggtat
     3601 ttctccatgt aaaccatatc gttgctgaaa gcagctttcg cttccgcacg ggtcatggag
     3661 atggattgtg ccagttcagc gtcgccgcgc actacgcgca taccgcgacc gccgccgccg
     3721 ccggaggctt tgataatcac cggataacca atgcgtttag caatggcgcg gtttttatcc
     3781 atatcgtcgc ccagcgggcc gtcagaacca ggtacgcaag gaacgcccgc ttttttcatc
     3841 gccgcgattg cggatacttt gtcgcccatc aggcgaatgg tttctgcttt cgggccgatg
     3901 aagataaagc cggagcgttc aacctgctcg gcaaagttgg cgttctcgga gaggaagccg
     3961 taacccggat ggattgctac tgcgccggtg atttcagcgg cgctgatgat tgccgggatg
     4021 ttcagataac tttttactga cggagcaggg ccaatacaga ccgtttcatc tgccagtaat
     4081 acgtgtttta gatcgcgatc cgtgctggag tgcacagcga cagtcttgat gcccagttct
     4141 ttacaggcac gaagaatacg caatgcaatc tcgccgcggt tggcaataac aattttatcc
     4201 agcatgttcg cctcgttact cgatgacgac cagcggctcg tcaaattcta ccggttgtcc
     4261 actttcgacc agaattgctt tcacggtacc ggatttgtcc gcttcgatct ggttcatcat
     4321 tttcatggct tcaacgatgc acagggtatc gcccacgttg actttctgac ccacttcgat
     4381 gaacgctttt gcgtccgggc ttggggtgcg gtagaaagta ccaaccatcg gggaacgtac
     4441 gatgtgacca ctgatttccg ctgctgctgg cgcttccatg gaaggaacgg tcgccggagc
     4501 ggctgcgtta gatttagctg gctgctgcat cattggtgca gcgtaagctt gttgcatcac
     4561 agggaaactt gcggcaggag ctgcacggct aatgcgtact gactcttcgc cttcagaaat
     4621 ttccagttcg gagatgcctg attcttcaac cagctcgatc agttttttaa tcttacgaat
     4681 atccatgagt gggttccgta ctctttgttt agtgtgattg tgacaggcgt ttcaccgccg
     4741 tctgtaaagc gtatgccccg acagctgtat gcatagcgat aaattccagc aggccgggac
     4801 gccggtctat tttgccccag agtgtcaatg ttagacttga cggacattgt gcagcgatct
     4861 gctgctatcc tccggcaaaa aacaaaatat accttcgttg cgccaatgtc accttttccg
     4921 cagcgcaaaa actgcgtcag ggaggacgag gtcgcacatt ataacgattt cgtagcaatt
     4981 ggcagctaaa tactggtctt atcagggaat ataatcaaca gctaacatgt aaataacctt
     5041 caacaccgtg taatttgcaa caagccgcac aattcacgaa attagcgcca tcatcgacgg
     5101 aacttcttat aacgtaaggt gaaaagctga aaagccagcc ctgcgaagat gaggggctgc
     5161 aatgagataa tcttcacaga ccacaaataa tgtagggatg ccaggatcgc gacaagatag
     5221 acgacattac gcagcggttg ctggggatta cccaattttc actgcatcgt ctggatagat
     5281 gtgaatgcta aagcacgcaa aatcaaccaa ctgacaattc tcagcattaa ataggttcga
     5341 gtggttaatt tgtagtcaaa taaaatcaga ttattcaccc catggtacca gaaatcagta
     5401 acaggttaag cgtagtgtcg cccaggcaaa gcaccataaa cctaacaggc gacggaggcg
     5461 tatcaataat ggctgtttag aggagtatca atgcgcatca atttgtggtc agtcgaaatc
     5521 atgactttcc agtttggatg atgttgtaca acttcctgac caaggtgttc gacgaaagct
     5581 gtccggctaa ccgcttttcc cgttaaccga ttgaataatg atgtcaaaat gagttccctc
     5641 tcttattatt ccttgctaaa tggttagtta accttcacca gcgtgcgacc ctggatctgg
     5701 ttattaatga tggcctcggc aaagttcggt gcctctgaca gagatatctc ttttgccgcc
     5761 tgggtataga atgattccgg taaatcggcg accagtcgct gccaggcttg tgcgcggcgt
     5821 tctggtggcg tcattactga atccacccct tgcaaacgga cattacgcag aataaatggc
     5881 atgaccgtgg ttggcagagt aaaaccaccc gccagaccac aggccgccac gcagccgccg
     5941 taattcattt gcgccagcac ttttgccagc actttgtcgc caacagtgtc aattgcccca
     6001 gcccagactt gtttttccag aggacgggat tcggcaaact catcacgagg gagaataccg
     6061 ctggcaccta aacttttcag atattcatgg gtactttcgc gaccggaaac ggcaacgacc
     6121 tgataaccca acttatgcag cagcgccacg gcggtactgc cgacgccacc actggcaccc
     6181 gtcacgacaa tctcaccgtc ctgcgggcga acaccggcat cttccagcgc catcacacac
     6241 agcatggcgg taaaaccggc agtaccgata atcattgctt tacgcgcgtc cagcccttgc
     6301 ggcatggcaa ccagccagtc acctttcact cgcgcctgct ccgccagccc accccagtgg
     6361 ttttcaccaa cgccccagcc agtgagtaac acctcctgac cggcatgaaa acgcggatct
     6421 tcgctggtgc gtacagttcc ggcaaaatcg atcccaggaa tcatcggaaa attacggatg
     6481 atttttccct taccggtaat cgccagcgca tctttatagt tcaggctcga ccagtgaaca
     6541 tcgaccgtga catcgccctc cggcaggcga cgttcgtcca gagtctgtac tgatgcgaga
     6601 gttttgccgt cctgctgttc taaaagtaac gcctgcataa gtggtcctca tgtgcatgat
     6661 ggattggaaa ataatttctg aagactatac tcgctaatgg aaaagcaaag ccgatgaagc
     6721 gcaataaatt gcgagataaa tctgatttgc tagtatgccc gcttcctcac tatcggagtt
     6781 aacacaagga tgagattaac gacgaaattt tcggcctttg ttacgctgct caccgggtta
     6841 acaatttttg tgactttgct gggctgttcg ctaagtttct acaacgccat tcagtataag
     6901 tttagtcatc gcgttcaggc ggtggcgacg gcgattgata cccaccttgt gtcgaatgac
     6961 ttcagcgcat taaggccaca aattaccgaa ttaatggtgt cagcagatat cgttcgtgta
     7021 gacctgctgc atggtgataa acaggtttat accctggcca gaaatggtag ttatcgtccg
     7081 gttggcacca gcgatctgtt tcgtgaactg agcgttccgt tgataaagca tccggggatg
     7141 tcgttgcgtc tggtttatca ggatccgatg ggcaactatt tccattcgtt gatgaccacc
     7201 gcgccgctca cgggggcgat tggctttatc attcttatgc tctttctggc ggtacgctgg
     7261 ttacaacggc aacttgccgg gcaagaattg ctggaaaccc gggctactcg tatcttaaac
     7321 ggtgagcgtg gctctaatgt gttgggaacc atctatgaat ggccgcccag aaccagcagt
     7381 gcgctggata cgctgcttcg tgaaattcag aacgcacgcg aacaacacag ccgtcttgat
     7441 acgctgatcc gctcttatgc cgcacaggac gtgaaaaccg gcctcaataa ccgactcttt
     7501 ttcgataatc agttagcaac gttactggaa gatcaggaga aagtaggtac ccacgggatc
     7561 gtgatgatga ttcgtctgcc ggatttcaat atgttgagcg atacctgggg gcacagccag
     7621 gttgaagaac agttcttcac tctgacgaat ctgctgtcga catttatgat gcgctaccct
     7681 ggcgcactgc tggcgcgtta ccaccgcagt gattttgctg cgctgttacc gcaccggacg
     7741 ttaaaagagg cagagagcat agccagtcag ttaatcaaag ccgtcgatac cttgccgaac
     7801 aataaaatgc tcgatcgcga cgatatgatc cacattggta tctgtgcctg gcgtagtggt
     7861 caggataccg agcaggtaat ggaacatgca gagtctgcca cgcgtaatgc gggattgcag
     7921 ggtggcaata gctgggctat ttatgatgac tcgttgcctg aaaaaggacg cggtaatgtt
     7981 cgctggcgta cgcttatcga gcaaatgctc agtcgcggcg gcccgcgcct ttatcaaaaa
     8041 ccggcggtta ctcgcgaagg tcaggttcat catcgcgaac tcatgtgccg catcttcgat
     8101 ggtaatgaag aggttagctc ggcggagtat atgccgatgg tcttgcagtt tggcttatcg
     8161 gaagagtatg accgtctgca aatcagccgt cttattccac tattgcgtta ctggccagag
     8221 gaaaatctgg cgattcaggt taccgttgag tcgctgattc gtccgcgttt tcagcgttgg
     8281 ctgcgcgata cgttaatgca atgtgaaaaa tcacaacgaa aacgcataat tattgaactt
     8341 gcagaggccg atgtaggtca acatatcagt cgtttacaac ctgttattcg tttagtgaat
     8401 gctttagggg tacgggtagc cgtcaaccag gctggtttga cgctggtaag caccagttgg
     8461 atcaaagaac ttaatgttga gttactcaag ctccatccgg ggctggtcag gaacattgag
     8521 aagcgaacgg agaaccagct gctggttcaa agcctggtgg aagcctgctc cgggaccagc
     8581 acccacgttt acgccaccgg cgtgcgttcg cgaagcgagt ggcagaccct gattcagcgc
     8641 ggtgttacag gcgggcaagg ggattttttc gcgtcctcac agccacttga tactaacgtg
     8701 aaaaaatatt cacaaagata ctcggtttaa cctgccgttt aatccgtttt cacgtagaat
     8761 aacgcgcgct gcgtctcatg ggagtgtgct tgtctgctcg ccagattgtt gcagcacata
     8821 tgcagatgaa tgaccttacg cggttgcaaa caggcgagga atgctgctga tgcattaagc
     8881 ctttctggac tcaggcagag atttgtaaca aaggaaacga actgcactaa ttttcaccgt
     8941 agcagatgat ttttgcgcct tgtcgctgct gcgtgtggtt ggtaaagtaa gcggattttc
     9001 ttttccgccc cagctttcag gattatccct tagtatgttg aaaaaatttc gtggcatgtt
     9061 ttccaatgac ttgtccattg acctgggtac tgcgaatacc ctcatttatg taaaaggaca
     9121 aggcatcgta ttgaatgagc cttccgtggt ggccattcgt caggatcgtg ccggttcacc
     9181 gaaaagcgta gctgcagtag gtcatgacgc gaagcagatg ctgggccgta cgccgggcaa
     9241 tattgctgcc attcgcccaa tgaaagacgg cgttatcgcc gacttcttcg tgactgaaaa
     9301 aatgctccag cacttcatca aacaagtgca cagcaacagc tttatgcgtc caagcccgcg
     9361 cgttctggtt tgtgtaccgg ttggcgcgac ccaggttgaa cgccgcgcaa ttcgtgaatc
     9421 cgcgcagggc gctggtgccc gtgaagtctt cctgattgaa gaaccgatgg ctgccgcaat
     9481 tggtgctggc ctgccggttt ctgaagcgac cggttctatg gtggttgata tcggtggtgg
     9541 taccactgaa gttgctgtta tctccttgaa cggcgtggtt tactcctctt ccgtgcgcat
     9601 tggtggtgat cgtttcgacg aagctatcat caactatgtg cgtcgtaatt acggttctct
     9661 gatcggtgaa gccaccgcag aacgtatcaa gcacgaaatc ggttcggctt acccgggcga
     9721 tgaagtccgt gaaatcgaag ttcgtggccg taacctggca gaaggtgttc cacgcggttt
     9781 taccctgaac tccaacgaaa tcctcgaagc actgcaggaa ccgctgaccg gtattgtgag
     9841 cgcggtaatg gttgcactgg aacagtgccc gccggaactg gcttccgaca tctccgagcg
     9901 cggcatggtg ctcaccggtg gtggcgcact gctgcgtaac cttgaccgtt tgttaatgga
     9961 agaaaccggc attccagtcg ttgttgctga agacccgctg acctgtgtgg cgcgcggtgg
    10021 cggcaaagcg ctggaaatga tcgacatgca cggcggcgac ctgttcagcg aagagtaatc
    10081 ggatgcaggc aggggaagcg tctgtttacc ctgcctggtc tgatacgaga atacgcataa
    10141 cttatgaagc caatttttag ccgtggcccg tcgctacaga ttcgccttat tctggcggtg
    10201 ctggtggcgc tcggcattat tattgccgac agccgcctgg ggacgttcag tcaaatccgt
    10261 acttatatgg ataccgccgt cagtcctttc tactttgttt ccaatgctcc tcgtgaattg
    10321 ctggatggca tatcgcagac gctggcctcg cgtgaccaac tagaacttga aaaccgggcg
    10381 ttacgtcagg aactgttgct gaaaaacagt gaactgctga tgcttggaca atacaaacag
    10441 gagaacgcgc gtctgcgcga gctgctgggt tccccgctgc gtcaggatga gcagaaaatg
    10501 gtgactcagg ttatctccac ggttaacgat ccttatagcg atcaagttgt tatcgataaa
    10561 ggtagcgtta atggcgttta tgaaggccag ccggtcatca gcgacaaagg tgttgttggt
    10621 caggtggtgg ccgtcgctaa actgaccagt cgcgtgctgc tgatttgtga tgcgacccac
    10681 gcgctgccaa tccaggtgct gcgcaacgat atccgcgtaa ttgcagccgg taacggttgt
    10741 acggatgatt tgcagcttga gcatctgcca gcgaatacgg atattcgtgt tggtgatgtg
    10801 ctggtgacct ccggtctggg cggtcgtttc ccggaaggct atccggtcgc ggttgtctct
    10861 tccgtaaaac tcgataccca gcgcgcttat actgtgatcc aggcgcgtcc gactgcagga
    10921 ctgcaacgtt tgcgttatct gctgctgctg tggggcgcgg atcgtaacgg cgctaacccg
    10981 atgacgccgg aagaggtgca tcgtgttgct aatgaacgtc tgatgcagat gatgccgcag
    11041 gtattgcctt cgccagacgc gatggggcca aagttacctg aaccggcaac ggggatcgct
    11101 cagccgactc cgcagcaacc ggcgacagga aatgcagcta ctgcgcctgt tgcgccgaca
    11161 cagcccgctg ctaatcgctc tccacaaagg gctacgccgc cgcaaagtgg tgctcaaccg
    11221 cctgcgcgtg cgccgggagg gcaatagtgg cgagctatcg tagccaggga cgctgggtaa
    11281 tctggctctc tttcctcatt gcgctgttgc tgcaaatcat gccctggccg gataacctga
    11341 ttgttttccg gccaaactgg gtgttactta tcttgttgta ttggatcctg gccttgcccc
    11401 atcgcgtaaa tgtgggcaca ggttttgtga tgggtgccat actggatctg atcagcggct
    11461 cgatgcttgg cgtacgcgta ttggcgatga gcatcattgc ttacctggtg gcgctgaaat
    11521 accagctttt ccgcaacctc gcattatggc agcaggcgct ggtcgtcatg ttgctttcgc
    11581 tggtggtgga tattattgtt ttctgggcag agtttttagt gattaacgtc tctttcagac
    11641 cggaagtgtt ctggagtagt gtagtcaatg gggtgctctg gccgtggatt ttcttgctga
    11701 tgcgtaaagt ccgtcagcag tttgcagtgc aataaaggtt tctatgactt ctctgtattt
    11761 agcttccggt tctccgcgtc gtcaggagtt acttgcgcaa cttggcgtga cctttgaacg
    11821 tattgttacg ggcattgagg agcagcgtca gccgcaggag agcgcgcagc agtatgttgt
    11881 gcgtctggca cgcgagaaag cacaggcggg tgtcgcgcaa acggcacagg atctcccggt
    11941 gctgggtgcg gatattatcg ttatcctgaa cggagaagtg ctcgagaaac cgcgcgacgc
    12001 agagcatgcg gcgcagatgt tgcgcaaatt atcgggtcag acccatcagg tgatgacggc
    12061 agtggcgttg gccgacagcc agcacattct cgattgcctg gtggtcaccg atgtgacttt
    12121 cagaacgtta acagacgaag acatcgcggg ctatgtcgcc agcggtgaac cgttagataa
    12181 agcaggtgca tacggtattc aggggctggg tggctgtttt gtcaggaaga taaatggcag
    12241 ctatcacgcc gtagtcggct taccgctggt tgaaacgtat gaattattaa gtaattttaa
    12301 cgcactgcgt gagaaaaggg ataaacatga cggctgaatt gttagtaaac gtaacgcctt
    12361 cggaaacgcg agtggcgtat attgatggcg gtattctgca ggaaattcat attgaacgtg
    12421 aggcgcgacg cggaatagta ggcaatatct acaagggtcg tgtaagtcgt gtacttccgg
    12481 gtatgcaggc ggcttttgta gatattgggc tggataaagc cgcgtttctt catgcatccg
    12541 acatcatgcc gcataccgaa tgtgtggcgg gtgaagaaca aaagcaattc acggtgcgcg
    12601 acatctcgga actggttcgt caggggcaag atctgatggt gcaggtggtg aaagatccgc
    12661 ttggcactaa aggtgcgcgc ctgaccaccg atatcacgct gccttctcgc tatctggtat
    12721 ttatgccagg ggcttctcac gttggggttt cccaacgaat tgaaagcgaa tcagaacgtg
    12781 aacgtctgaa aaaagtggtc gcagagtatt gcgacgagca gggcgggttt atcatccgca
    12841 ccgcagcgga aggggttggc gaggctgaac tggcctccga tgccgcttat ctgaaacgcg
    12901 tctggaccaa agttatggag cgtaaaaagc gcccgcagac ccgttatcag ctgtacggcg
    12961 aactggcgct ggcgcagcgt gttctgcgtg attttgccga cgccgaactg gaccgcattc
    13021 gcgttgactc acgcctgact tacgaagcat tgctggagtt tacctcggag tacattcccg
    13081 agatgaccag caagctggag cattacaccg gacgccagcc gattttcgat ctctttgatg
    13141 tcgaaaacga aatccagcga gcgttggaac gcaaagtaga actgaaatcc ggtggttatc
    13201 tgattatcga ccagaccgaa gcgatgacca ccgtggacat caataccgga gcgtttgtcg
    13261 gtcatcgcaa tctggacgac actattttca ataccaatat tgaagcgacg caggctatcg
    13321 ctcgccagtt acggttgcgt aatctgggcg ggattatcat tattgatttc atcgatatga
    13381 ataatgaaga tcaccgccgc cgcgtgctgc attcgctgga gcaggcgttg agcaaagacc
    13441 gggtgaaaac cagcgttaat ggtttttcgg cgctggggct ggtggagatg acgcgtaaac
    13501 gcacccgcga aagcattgag cacgtactgt gtaacgaatg cccaacctgc cacggtcgcg
    13561 gaacggtgaa aaccgtggaa acggtatgct atgaaatcat gcgcgagatt gttcgtgtcc
    13621 accatgctta cgactccgac cgtttcctgg tctatgcttc tccggcagta gccgaagcct
    13681 tgaaaggcga agagtcacac tcgctggcgg aagtggaaat tttcgttggc aaacaggtta
    13741 aagtacaaat tgaaccgctc tataaccagg agcagtttga cgtcgtaatg atgtaaacag
    13801 atgctgggcc gccatccggc aaagggtttt tgagtcacat ttttagcaga caaggagtga
    13861 cgggtgaggc gattgccggg gattttactg cttactggag ccgcgctcgt tgtgatcgct
    13921 gccctgctgg ttagcggcct gcgtattgct ttaccgcatc ttaacgcctg gcgtccggaa
    13981 atcctcaaca aaatagaatc cgcgactggc atgccggtag aagccagtca gctctcagcc
    14041 agctggcaga attttggccc gacgcttgaa gcacacgaca tccgtgcaga actaaaagat
    14101 ggcggcgaat tttcggttaa acgcgttact ctggcgctgg atgtctggca gagcctgtta
    14161 catatgcgct ggcagtttcg cgacctcact ttctggcagc tgcgctttcg caccaacact
    14221 cctatcacca gcggtggtag cgatgacagt ctggaagcca gtcacatcag cgatctgttt
    14281 cttcgtcaat ttgaccattt cgatctccgc gacagtgaag tcagttttct gacgccatcc
    14341 ggtcagcgcg ccgagctggc gatcccacaa ctcacctggc tgaacgatcc acgtcgacac
    14401 cgtgcggaag gcctggtaag cctctccagc cttaccggac agcacggcgt gatgcaggtg
    14461 cgcatggatt tgcgcgatga tgaggggttg ttaagcaatg gtcgcgtctg gctacaggcg
    14521 gatgacatcg acctgaagcc gtggctcggt aaatggatgc aggacaatat tgctctggaa
    14581 acggcacagt tctcccttga aggctggatg acgatcgaca aaggcgatgt aaccggcggt
    14641 gacgtctggc tgaaacaggg cggtgccagc tggttgggcg agaagcaaac gcatacgctg
    14701 tcggtggata atctgaccgc gcatattacg cgtgaaaatc cgggctggca gttctctatt
    14761 cccgatacac ggatcacgat ggacggcaaa ccctggccga gcggagcatt gacgctggcc
    14821 tggataccgg aacaggacgt tggcggcaaa gacaataaac gcagtgacga actccggatt
    14881 cgcgctagta atctggagct ggcaggcctg gagggcatac gcccgctggc cgcgaaactt
    14941 tcacctgcac tgggtgatgt ttggcgctcc acacaaccga gcggcaagat taacactctg
    15001 gcgctggata tcccgcttca ggcggcagac aagacccgtt ttcaggcatc gtggagcgat
    15061 ctggcctgga agcaatggaa attattaccg ggtgcggaac acttctccgg gacgctttcc
    15121 ggcagcgttg aaaatggttt gcttaccgcg tcgatgaagc aggcaaagat gccttacgaa
    15181 acggtattcc gtgcgccact ggaaatcgcc gacggccagg caactataag ctggctgaac
    15241 aatgacaaag gtttccagct ggatgggcgt aatattgacg ttaaagccaa agccgtccat
    15301 gcgcgcggcg gttttcgtta cctgcaacct gctaacgatg aaccctggct gggtattctg
    15361 gctggcatca gtaccgatga tggttcacaa gcctggcgct atttcccgga aaacttgatg
    15421 ggtaaagacc tggttgatta tttaagtggt gcgattcagg gcggtgaagc ggataacgcg
    15481 acgctggttt atggtggcaa tccgcaactc ttcccctata aacacaacga aggtcagttt
    15541 gaagtgctgg tgccgctgcg caacgcgaag tttgccttcc agccggactg gcctgcatta
    15601 actaaccttg atattgaact ggactttatt aacgacggtt tatggatgaa aaccgatggc
    15661 gttaatctgg gcggcgtgcg cgcgagtaat ctcaccgcag tgatccctga ctactcaaaa
    15721 gaaaaactgc tgattgacgc tgacattaaa ggtccgggta aagccgttgg cccttacttt
    15781 gatgagacac cgctgaaaga ttctctgggt gcgaccctgc aagaactcca gctcgacggc
    15841 gatgtgaatg ctcgcttaca tcttgatatc ccgctgaacg gcgaactggt aaccgcgaaa
    15901 ggtgaagtga cgctgcgtaa taacagtctg tttatcaaac cactcgacag catcctgaaa
    15961 aatttgagcg gtaaattcag ctttatcaat ggcgatctgc aaagtgaacc actgacagca
    16021 agctggttta atcagccgtt gaacgtggat ttttccacca aagaaggggc aaaagcctac
    16081 caggttgcgg tgaatctcaa cggtaactgg caaccggcga aaaccggcgt tctgcctgaa
    16141 gcggtgaacg aagcattgag tggcagcgtg gcgtgggatg gtaaagtggg cattgatctg
    16201 ccttatcatg ctggcgcgac ctataacgta gagctgaacg gcgatctgaa taatgtgagt
    16261 agtcacttac cttcaccgtt agccaaacct gcgggtgaac cactagcggt aaacgttaag
    16321 gttgatggca atctcaacag ctttgaatta accggacagg ctggtgcgga taaccatttc
    16381 aatagccgct ggttgcttgg tcaaaagctg acgctcgatc gtgctatttg ggcggcagac
    16441 agtaaaacgc tcccgccgtt gccggaacaa agtggcgttg aacttaatat gccgccgatg
    16501 aatggtgccg agtggctggc cctgttccag aaaggcgctg cggagagtgt cggtggtgca
    16561 gcgagtttcc cacaacacat aacgttacgt acgcctatgt tgtcactggg aaatcagcaa
    16621 tggaataacc tgagtattgt ttcgcaaccg acggcaaatg gcaccctggt tgaggcgcaa
    16681 gggcgtgaaa tcaacgccac gctagcgatg cgtaataacg cgccgtggct ggcgaatatc
    16741 aaatatcttt attacaaccc gagcgtggcg aaaactcgtg gtgattcaac gccgtcatca
    16801 cctttcccga caacggagcg cattaacttc cgtggctggc cggacgcaca aatacgatgc
    16861 acagagtgct ggttctgggg gcaaaaattc ggtcgcattg acagtgatat caccatttct
    16921 ggcgatacgt taacgctgac caatggactg attgatactg gtttctcgcg gcttactgcc
    16981 gatggtgaat gggttaataa tccggggaat gaacgtacct cgctgaaagg aaaactgcgt
    17041 gggcagaaaa ttgatgccgc cgcagaattt tttggtgtca cgacgcccat acgccagtcg
    17101 tcatttaatg tggattacga tttacactgg cgcaaagcac cctggcagcc agatgaagcg
    17161 acgttgaatg gcatcattca tactcaactg ggtaaaggcg aaattaccga aatcaatacc
    17221 ggacatgccg ggcaattgct gcgcttattg agcgtagatg ccctgatgcg taagctgcgt
    17281 tttgatttca gagacacttt tggcgaaggg ttctattttg actccattcg cagcaccgcg
    17341 tggattaaag acggcgttat gcacaccgac gacacgctgg tggatggcct ggaggcggat
    17401 atcgccatga aagggtcggt aaatctggta cgccgcgacc tgaatatgga agcggttgtc
    17461 gcaccagaga tttctgcgac ggtgggcgtg gctgcggctt ttgcggttaa ccccattgtt
    17521 ggcgcggcag tgtttgccgc cagtaaagtg ctggggccgc tgtggagcaa agtctccatt
    17581 ttgcgctatc acatttcggg tccgctggac gatccgcaaa tcaacgaagt gttgcgccaa
    17641 ccgcgtaaag aaaaagcgca atgatttgac gagggcgcgt aattgcccca atctcatatg
    17701 ataatcgttg ccaaaggcca acgagccgga acataactgt aggccggata gggcgttcac
    17761 gccgcatccg gcagccgtaa aaaatcctct actgcagtaa ctaacgagta gcaaaaacga
    17821 tgagtctgaa cctggtaagt gaacaattgc tagcggcgaa cggcctgaaa catcaggact
    17881 tgttcgcgat cctcggtcaa ctggccgaac gtcgccttga ttatggcgat ctctattttc
    17941 agtcgagcta tcacgaatcc tgggttttag aagaccgcat tattaaagat ggttcttaca
    18001 acatcgatca gggcgttggc gtgcgtgcaa tcagcggtga aaaaaccgga tttgcttacg
    18061 ctgaccaaat cagcctgctg gcgctggaac agagtgcgca agcggcgcgc accatcgtcc
    18121 gtgatagtgg tgatggcaaa gtacagacgc tgggcgcggt agagcatagc ccgttgtata
    18181 cctcggtaga tccgctgcaa agcatgagcc gtgaagagaa gctggatatc ctgcgtcgcg
    18241 tcgataaggt tgctcgcgaa gcggacaagc gcgtacagga agtgactgcc agcctcagtg
    18301 gtgtctatga attaattctg gttgcggcca ccgacggcac gctagcggcg gatgtccgtc
    18361 cgctggtgcg tctttccgtg agcgttctgg tcgaagaaga tggcaaacgc gaacgcggtg
    18421 ccagtggcgg cggcggtcgt tttggttatg agttcttcct tgccgatctt gacggcgaag
    18481 tccgtgcgga tgcatgggca aaagaagcag tgcgtatggc gctggtcaat ctttctgccg
    18541 ttgctgcacc agcgggcacc atgccggtag tacttggcgc aggttggccg ggcgtgctgt
    18601 tgcatgaagc ggtaggtcac ggtctggaag gcgacttcaa ccgccgtggc acttcagtat
    18661 ttagtggaca ggtcggggag ctggtggctt cagaactgtg taccgtggtt gatgacggca
    18721 cgatggtcga tcgccgtggt tcggtggcga ttgatgatga aggtacgcca ggccagtaca
    18781 acgtgctgat tgagaacggc attctgaaag gctacatgca ggataaactc aacgcgcgtt
    18841 tgatggggat gacgccgact ggcaacggtc gccgtgaatc ctacgcccat ctgcccatgc
    18901 cgcgtatgac caacacctat atgctgccgg gtaaatcgac cccgcaagaa attattgaat
    18961 ccgttgagta cggtatctat gcaccgaact ttggtggcgg tcaggtggat atcacctccg
    19021 gcaaattcgt tttctccact tcagaagcat atctgattga aaacggtaaa gtaacgaagc
    19081 cggtgaaagg cgcaacgttg attggttccg gtatcgaaac catgcagcag atttcgatgg
    19141 ttggcaacga cctgaaactg gataacggcg tgggtgtctg cggtaaagaa gggcaaagtt
    19201 tgccggttgg cgtgggccag ccaacgttga aagtcgataa cctgactgtt ggcggtactg
    19261 cgtaataatt tcattatttt cagaaggata attaatcttt ctacgtgcac gaacggtccc
    19321 ctcgctcctc tggggttagg gtgaggggaa cccgttggca caggtttgta gaacgtaaca
    19381 gtacaatatg aattacttct ctttcccgcg cccgtgcatc tcctgaaaca atttaccgac
    19441 ctcaacaaaa taatccgtca gcgagttgat cacgacctgt accttcagcg gcagcttatc
    19501 tttttcggta tataacgcat aaaccgggcg tggatctgac tgataacgcg gcagcaggat
    19561 ctccagctcc ccacgattga tctcgttgat cacccacatc agcggcacgt aggcgatccc
    19621 ggcacccgcc gttagccagc gcaccagcgt catcggatca ttagtcacaa atctcccttg
    19681 cgggatcagg cgagtcgaga tcccttccgg tgcaatcagt tcaaattcat tgtcgggccg
    19741 cacgctgtat tcaagccatg aatggctact taaatcggcg ggtttttccg gtatgccgta
    19801 ttgtgtgaga tagcttttcg cggcgcacac caccattggc atcgcgccaa gacggcggga
    19861 aaacaggctg gaatcctgca acgcgccgac gcggatcacc acatccagac cgtcggcaat
    19921 caggtcgggg gctggaattc cggtaaccag attggcgctc aaacctgggt attctttcag
    19981 cattttggct gtcagcccgg cgagaacatt ttgtgccata gttgaagaac agccaatgcg
    20041 tagcgtcccg atgggggtgt tattgaaggc atacagttgc tcatgaacat cctgcacttc
    20101 atgaagcata cgacggcagc cctggtagta aattctaccg gcttcggtca ggccaatgct
    20161 gcgtgtgcta cggtttaaca gctttacctg caactcatct tccagttttg acaccgtctg
    20221 actgatggac gaaacgctca tctgtagctg tctggcggcg gcggtaaaag agccaaattc
    20281 aactactttg gcaaacaccg acatgcgttt tagtcgttcc attattcact ctgacttaaa
    20341 agtgatttag atcacataat atagataaca gcataacagt tacgctaata tattaaatat
    20401 caatctacag caatgttgct ctcgcccggc tttccatgcc cttctctgcg gcgacagatg
    20461 ctgaaaataa taacgcctgc tctctctata caaccaaggt caacatgagt ctgtttcccg
    20521 ttatcgtggt gtttgggctg tccttcccac cgatattttt tgaattgctt ttatcactgg
    20581 cgattttctg gctggtgcgc cgggtacttg tgccaacagg tatctacgac tttgtctggc
    20641 atccggcgtt gttcaacacc gcgctctatt gctgcttgtt ttatttgata tcgcgactgt
    20701 tcgtttgagg ttgaagtgaa aacactaata agaaaattct cccgtacggc catcacggtc
    20761 gtattagtca ttctggcctt catcgcaatt tttaatgcct gggtctatta caccgaatcc
    20821 ccctggacgc gtgacgcgcg ctttagcgct gacgtcgttg cgatcgcgcc ggacgtttct
    20881 ggactcatta cccaggtgaa tgttcatgat aaccagctgg tgaaaaaagg acagatactg
    20941 ttcaccatcg accagccgcg ctatcaaaag gcgcttgagg aagcgcaagc cgatgttgct
    21001 tattatcagg tactggcaca ggagaaacgc caggaggccg gacgtcgtaa ccgtctcggt
    21061 gtgcaggcga tgtctcgcga agagatcgac caggctaaca acgtactaca aacggttctg
    21121 catcagttag cgaaagcgca ggcgacccgc gatctggcaa aactggatct tgaacgcacg
    21181 gtgatccgag cgccagcaga tggctgggtg accaacctca acgtctatac cggtgagttt
    21241 attactcgag gatcaacggc ggttgcgctg gtgaaacaga actccttcta tgtactggcc
    21301 tatatggaag aaactaagct ggaaggggtg cgtccggggt atcgtgcaga gatcacgccg
    21361 cttggcagta acaaagtgct gaaagggact gttgatagtg ttgccgctgg ggtcaccaac
    21421 gccagcagca cgcgtgacga caaagggatg gcgaccatcg actctgacct tgaatgggtg
    21481 cgtttggcgc aacgtgttcc ggttcgtatt cgtctcgaca accagcaaga gaacatctgg
    21541 cctgcgggca ccactgctac agtggtggtc actggcaaac aagatcgcga cgaaagccag
    21601 gattcgttct tccgtaaaat ggcccatcgc ctgcgtgagt ttggttaatc acgatgggta
    21661 ttttctccat tgctaaccaa catattcgct ttgcggtaaa actggcgacc gccattgtac
    21721 tggcgctgtt tgttggcttt cacttccagc tggaaacgcc acgctgggcg gtactgacag
    21781 cggcgattgt tgccgccggt ccggcctttg ctgcgggagg tgaaccgtat tctggcgcta
    21841 ttcgctatcg tggctttttg cgcatcatcg gcacatttat tggctgtatt gccggactgg
    21901 tgatcatcat tgcgatgatc cgcgcaccat tattgatgat tctggtgtgc tgtatctggg
    21961 ccggtttttg tacctggata tcctcgctgg tacgaataga aaactcgtac gcgtgggggc
    22021 tggccggtta taccgcgctg atcattgtga tcaccattca gccggaacca ttgcttacgc
    22081 cgcagtttgc cgtcgaacgt tgtagcgaga tcgttatcgg tattgtgtgt gcgattatgg
    22141 cggatttgct cttttctccg cgatcgatca aacaagaagt agatcgagag ctggaaagtt
    22201 tgctggtcgc gcaatatcaa ttaatgcaac tctgtatcaa gcatggcgat ggtgaagttg
    22261 tcgataaagc ctggggcgac ctggtgcgac gcaccacggc actacaaggc atgcgcagca
    22321 acctgaatat ggaatcttcc cgctgggcgc gggccaatcg acgtttaaaa gcgatcaata
    22381 cgctatcgct gacgctgatt acccaatcct gcgaaactta tcttattcag aatacgcgcc
    22441 cggaattgat cactgatact ttccgcgaat tttttgacac gccggtagaa accgcgcagg
    22501 acgtccacaa gcagctcaaa cgcctgcgga gagttatcgc ctggactggg gaacgggaaa
    22561 cgcctgtcac catttatagc tgggtcgcgg cggcaacgcg ttatcagctt ctcaagcgcg
    22621 gcgttatcag taacacaaaa atcaacgcca ccgaagaaga gatcctgcaa ggcgaaccgg
    22681 aagtaaaagt agagtcagcc gaacgtcatc atgcaatggt taacttctgg cgaaccacac
    22741 tttcctgcat tctgggcacg catttctggc tgtggacggg ctggacttcc ggcagtggtg
    22801 caatggtgat gattgcggtc gtgacgtcac tggcaatgcg tttgccgaat ccacgcatgg
    22861 tggcgatcga ctttatctat gggacgctgg ccgcgctgcc gttagggctg ctctactttt
    22921 tggtgattat ccctaatacc caacagagca tgttgctgct gtgtattagc ctggcagtgc
    22981 tgggattctt cctcggtata gaagtacaga aacggcgact gggctcgatg ggggcactgg
    23041 ccagcaccat aaatattatc gtgctggata acccgatgac tttccatttc agtcagtttc
    23101 tcgacagcgc attaggacaa atcgtcggct gtgtgctcgc gttcaccgtt attttgctgg
    23161 tgcgggataa atcgcgcgac aggactggac gtgtactgct taatcagttt gtttctgccg
    23221 ctgtttccgc gatgactacc aatgtggcac gtcgtaaaga gaaccacctc ccggcacttt
    23281 atcagcagct gtttttgctg atgaataagt tcccagggga tttgccgaaa tttcgcctgg
    23341 cgctgacgat gattatcgcg caccagcgcc tgcgtgatgc gccgatcccg gttaacgagg
    23401 atttatcggc gtttcaccga caaatgcgcc gcacagcaga ccatgtgata tctgcccgta
    23461 gcgatgataa acgtcgtcgg tactttggcc agttgctgga agaactggaa atctaccagg
    23521 aaaagctacg catctggcaa gcgccaccgc aggtgacgga accggttcat cggctggcgg
    23581 ggatgctcca taagtatcaa catgcgttga ccgatagtta agtcaaaacc gacgccaaaa
    23641 gcgtcggttt tttcatggct atacttagcg atgcacggca gaactcgccg cgaaacgtga
    23701 cggtggcaac agatgaatat ttatgccttt gattttgatg agattgagag tcaggaggat
    23761 ttttatcgtg actttagcca aacctttggt ctggctaaag ataaggtacg cgatctcgac
    23821 tcactatggg atgtgttaat gaacgatgtc ctgccgctac cacttgagat tgaatttgtt
    23881 catctgggag agaaaacgcg tcgccgtttt ggcgcgttaa tattgctgtt tgatgaggca
    23941 gaggaagagc tggaagggca tttgcgtttt aatgttcgtc attagcgcaa aaaaaagccc
    24001 acgaaccggg ggcaatatcg tcggacaaaa cgatgagggt ttatttgtac agttcagccg
    24061 tagcgtgcca ggtgtcaccg ctacgagctt cagtaatctg gtaggccgtt gcgcctttct
    24121 cttccgcttt tttgttcagc atttcacgta tatccattgg cgaagacgcc acaccactta
    24181 cggatacggt cccgattgct tcacgatttt gtgcttgtgc agcatcaatg gagtcggcag
    24241 cgaatgcacc gaaagagaga acagaaagta cgcttaatgc agctacagtg gttttgattt
    24301 tcatgatttt tacctcgaca taatctttta gctgggcctt tgtttcgtga cccatatcac
    24361 aaaatcaagt atacactaat ta
//
LOCUS       DAZPAB010000042        24352 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZPAB010000042 DAZPAB010000000
VERSION     DAZPAB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.042"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..100)
                     /locus_tag="ACHUPG_001508"
     CDS             complement(<1..100)
                     /locus_tag="ACHUPG_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861173.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYL"
     gene            complement(193..765)
                     /locus_tag="ACHUPG_001509"
     CDS             complement(193..765)
                     /locus_tag="ACHUPG_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR0861174.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            983..1843
                     /gene="tesB"
                     /locus_tag="ACHUPG_001510"
     CDS             983..1843
                     /gene="tesB"
                     /locus_tag="ACHUPG_001510"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR0861175.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1892..3178)
                     /gene="amtB"
                     /locus_tag="ACHUPG_001511"
     CDS             complement(1892..3178)
                     /gene="amtB"
                     /locus_tag="ACHUPG_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR0861176.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3208..3546)
                     /gene="glnK"
                     /locus_tag="ACHUPG_001512"
     CDS             complement(3208..3546)
                     /gene="glnK"
                     /locus_tag="ACHUPG_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR0861177.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3727..5508)
                     /locus_tag="ACHUPG_001513"
     CDS             complement(3727..5508)
                     /locus_tag="ACHUPG_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR0861178.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5501..7273)
                     /locus_tag="ACHUPG_001514"
     CDS             complement(5501..7273)
                     /locus_tag="ACHUPG_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR0861179.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7303..7761)
                     /gene="decR"
                     /locus_tag="ACHUPG_001515"
     CDS             complement(7303..7761)
                     /gene="decR"
                     /locus_tag="ACHUPG_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFR0861180.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7915..8733)
                     /gene="cof"
                     /locus_tag="ACHUPG_001516"
     CDS             complement(7915..8733)
                     /gene="cof"
                     /locus_tag="ACHUPG_001516"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR0861181.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8833..10525
                     /locus_tag="ACHUPG_001517"
                     /pseudo
     CDS             8833..10525
                     /locus_tag="ACHUPG_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10590..11285
                     /gene="queC"
                     /locus_tag="ACHUPG_001518"
     CDS             10590..11285
                     /gene="queC"
                     /locus_tag="ACHUPG_001518"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR0861182.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11337..11735)
                     /gene="fadM"
                     /locus_tag="ACHUPG_001519"
     CDS             complement(11337..11735)
                     /gene="fadM"
                     /locus_tag="ACHUPG_001519"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR0861183.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11829..12200)
                     /locus_tag="ACHUPG_001520"
     CDS             complement(11829..12200)
                     /locus_tag="ACHUPG_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR0861184.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12351..14222)
                     /gene="ppiD"
                     /locus_tag="ACHUPG_001521"
     CDS             complement(12351..14222)
                     /gene="ppiD"
                     /locus_tag="ACHUPG_001521"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR0861185.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DVASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14414..14704)
                     /gene="hupB"
                     /locus_tag="ACHUPG_001522"
     CDS             complement(14414..14704)
                     /gene="hupB"
                     /locus_tag="ACHUPG_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR0861186.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14895..17249)
                     /gene="lon"
                     /locus_tag="ACHUPG_001523"
     CDS             complement(14895..17249)
                     /gene="lon"
                     /locus_tag="ACHUPG_001523"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR0861187.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17437..18711)
                     /gene="clpX"
                     /locus_tag="ACHUPG_001524"
     CDS             complement(17437..18711)
                     /gene="clpX"
                     /locus_tag="ACHUPG_001524"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR0861188.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18837..19460)
                     /gene="clpP"
                     /locus_tag="ACHUPG_001525"
     CDS             complement(18837..19460)
                     /gene="clpP"
                     /locus_tag="ACHUPG_001525"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR0861189.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19707..21005)
                     /gene="tig"
                     /locus_tag="ACHUPG_001526"
     CDS             complement(19707..21005)
                     /gene="tig"
                     /locus_tag="ACHUPG_001526"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR0861190.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21349..21666)
                     /gene="bolA"
                     /locus_tag="ACHUPG_001527"
     CDS             complement(21349..21666)
                     /gene="bolA"
                     /locus_tag="ACHUPG_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR0861191.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21971..22549
                     /locus_tag="ACHUPG_001528"
     CDS             21971..22549
                     /locus_tag="ACHUPG_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0861192.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22593..24068
                     /gene="ampG"
                     /locus_tag="ACHUPG_001529"
     CDS             22593..24068
                     /gene="ampG"
                     /locus_tag="ACHUPG_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR0861193.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5719 a         6706 c         5867 g         6060 t
ORIGIN      
        1 cgaggtaatc ccccagagaa gtcagtgggt tacgcagaga atcgtaacgg gataccagat
       61 caagagcctg tccaatgtgc ataaaaaaat ccggaaacaa gtgagcgttt ccggattctt
      121 acacagccac tggatcggtc aactgatcct taactgatcg gcattactcc gggcggagag
      181 ggttaacgtc gcttaatatt gcgtaggagc gggaactgcc gaagacggat tcacctgagt
      241 tggtgaagtc gaaggtacgg tagtcgttgc gccaccgctg gcctgaaccg gcacggcggt
      301 ttgctgtacc ggcaccaatg tcaggtcggc tttagttccg ccctggttga tcaccggctg
      361 aacggtatcg gtgataaata ccagtttgtc attcacggta atcgccgcac tcaacagaat
      421 acgcgcgttc ggctgaacat ctgccgggtt aaatggcaga acaaagctga atggtgactg
      481 tttaccttca gtacgcaccg ctttctgcgc cagcactttt gacggtgcat cggctaacga
      541 cgcgtcagaa agtgtcacgg tcagcacagc atcaggcggc agtgcgactt tctgacggat
      601 ccagacagta ccggagacat tcggttgctg gatagcaggt tgttgtgttg ctgaaataga
      661 cgtatttgca gccggggctg gcgtctgaat atccgcgctt ttatctgcgc aagccgccaa
      721 cgcaatcgca accgctaaac cactggccat gtgcacgagt ttcattcatt tctccttatt
      781 atcaatgcac cagcgggcta actttcctcg ccggaagagt ggttaacaaa atagtaacgt
      841 caacaagtgt ggcacacatc acgcatttct gcctgtaatt agcccgtaat tcagactatt
      901 tcacccatcg gaccagtggc aaatttgcgt tatactcaac tcactttggc ttgctgcggc
      961 agctttgtta ctggagagtt atatgagtca ggcgctaaaa aatttactga cattgttaaa
     1021 tctggaaaaa attgaggaag gactctttcg cggccagagt gaagatttag gtttacgcca
     1081 ggtgtttggc ggccaggtcg tgggtcaggc cttgtatgct gcaaaagaga ccgtcccgga
     1141 agagcggctg gtacattcgt ttcacagcta ctttcttcgc ccaggcgata gtaagaaacc
     1201 gattatttat gatgtcgaaa cgctgcgtga cggtaacagc ttcagcgccc gccgggttgc
     1261 tgctattcaa aacggcaaac cgatttttta tatgacggcc tctttccagg caccagaagc
     1321 gggtttcgaa catcaaaaaa caatgccgtc cgcgccagcg cctgatggcc tgccttcgga
     1381 aacgcaaatc gcccaatcgc tggcgcacct gctgccgcca gtgcttaaag ataaattcat
     1441 ctgcgatcgt ccgctggaag tccgtccggt ggagtttcat aacccactga aaggtcacgt
     1501 cgcagaacca catcgtcagg tgtggatccg cgcaaatggt agcgtgccgg atgacctgcg
     1561 cgttcatcag tatctgctcg gttacgcttc tgatcttaac ttcctgccgg tagctctaca
     1621 gccgcactgc atcggttttc tcgaaccggg gattcagatt gccaccattg accattccat
     1681 gtggttccat cgcccgttta atttgaatga atggctgctg tatagcgtgg agagcacctc
     1741 ggcgtccagc gcacgcggct ttgtgcgcgg tgagttttat acccaagacg gcgtgctggt
     1801 tgcctcgacc gttcaggaag gggtaatgcg taatcacaat taaaaaaata gccggaggtg
     1861 aaaaccgtcc ggctattttt tgcagtgctt gttacgcgtt ataggcattc tcgccgtggc
     1921 tgttgacgtc cagcccttct cgctcttgtt cttccggcac acgtaggcca accgtcagat
     1981 ccgccagttt gtagccaata aatgccacca caccggacca gacgatcgta atggcgatgc
     2041 tttccaactg taccagcaac tgatggccca tcgtcacacc ttcagcgaag cccacgccgc
     2101 ccagagaact ggcggcaaaa atcccggtca tgatacagcc gacaatgcca caaacgccgt
     2161 gcacaccgaa gacatcgcag ggatcatcca cccgcagcaa gcgtttgagc atggtaacgc
     2221 cccacaaacc cgccagacca gctaccacgc cgataatcaa cgcgccgcca accccaatgt
     2281 agccgcacgc tggcgtcacg ccgaccagac cggcaatcgc gccagaacac gctcccagca
     2341 gagaaggctt accacgcagc gcccattcac cgaagatcca gccaagaatt gccgccgccg
     2401 ttgcgaccac agtattcaca aatgccagtg ccgcgatttc attcgccgtg cccactgacc
     2461 cggcgttaaa gccaaaccaa ccgatataga gaatggcagt cccggtgaag accatcggca
     2521 ggttgtgcgg tttaaacgcc tctttaccga agcctacgcg tttgcctatc agatacgcgc
     2581 ccaccagacc ggcgattgct gcgttaatgt gcaccaccgt gccacccgcg aaatccagcg
     2641 caccgtgaga agccagcaaa ccaccgcccc acaccatatg cgcaatcgga atgtaagaga
     2701 gcgtcagcca taccaccacg aaaatcaaca cagctgagaa gcggattcgt tccgccagcg
     2761 ccccaactat caagcctacg gtaatgcagg caaacgatcc ctgaaacgct acgtggatat
     2821 actgataaat gctgcccatc accgccgtca gttcgatgtt ttttagcatc aaccagttaa
     2881 tgttgccgaa gaagttgttg ccctcaccaa acgccagcga ataaccgtaa accacccaga
     2941 gaatacagac cagtgcaaac gtcaccgtca cctgcgtcag catcgacagc acgtttttgc
     3001 cgcgaatcaa cccaccgtaa aacagggcaa tccccggaat agtcataaac agcaccagcg
     3061 cagtacaaat catcataaac gcattgtcgg ctttatcggc caccgcaggt gcagccatta
     3121 ccagtcccgg cagcatcgcc agtgaagcaa gcccagtttt tatcgtcgct atcttcattt
     3181 ttcgttcctg ttgctgtgtg ccagagatta cagcgccgct tcgtcggctt cgccggtacg
     3241 aatacgaatg acgcgttgca attcagcgac gaagattttg ccgtcgccaa tttttccggt
     3301 gtaagccgcc ttactgacga tatcgatcac ttcatcgagt tggtcatcag caatcgccac
     3361 atcaattttt acttttggca ggaagttgac gctgtattcc gccccccggt acagctcggc
     3421 atgccctttc tgacgcccga aacctttcac ttcggtgacg gtcaggccct gaataccaat
     3481 ggaagataac gcttcacgaa cgtcttccag cttgaatggt ttgattatca cggtcaccag
     3541 cttcatagat cccctccggt cagaattcgg taatggttct gctacacgaa gaaggtattg
     3601 caagcggtgt gccagaaatg aaaaacgaga aagagagcag gaagttaaga agattgaaaa
     3661 tgaaaacgca ctatgacagt gcacagtgcg ttacattttt gcaccaacga tgaaatattg
     3721 cgctattcag gcgctcaatg attcctcttc acgcacgctg gctgccagct cttcgcccgc
     3781 aagttgcagt tgatacatct gccagtagcg tccctgggcc gccagcagtt gctggtgagt
     3841 gccctgctcc acggcttgcc cacgatgaag caccagaatg gtgtcggcat caataatagt
     3901 tgataagcgg tgagcaatca ccaccagcgt ggtatgttca cgcaccgccg ccagagcatg
     3961 ttgaatcgcc tgttcagtac cggagtcaat gctggcggtt gcctcatcaa ggatcaggat
     4021 ttgcggcgtc tccaccagca cgcgcgccag tgccagcagt tgcttttgcc cgactgagag
     4081 attatttccc tgctcgccca gcggcgtata aatacaatca ctcatgctgc gcgccagctc
     4141 cgccagttgc acggtttcca gcgcctgcca gacgcgttct tcggagatat cccgccccag
     4201 cgtcacatta gcgaggaagg tatccgccag caccaccgga tcttgctgca ccattgccac
     4261 gccctggcgc agcgcgctat gacttagcga acttaatgga cgaccatcaa ggtgaatctc
     4321 accttccgtt agcgggtaat agcccatcaa taaactggcg agggtacttt taccactgcc
     4381 ggtatgcccg accagcgcca caaagttacg cgaaggcaca gagagattaa tgttctttag
     4441 caccagattg tcatcgcgat aagcaaatga cacgttatcg acttcgatgg tgccactctg
     4501 taacgggtaa tcatcattgc catattgctg gcgcggtccg tccatcagtt caaacacgcg
     4561 ctcaccagca acaacagcct gttgcagcat cgcctgttgc gtggtcagtt cgattaaggg
     4621 ttcgttaagt cgcccaagat agctgataaa cgcatacaac acgcccactt caatggtgcc
     4681 gctggcggag aagccaaaca gcatcaataa gccacaaaga atgagcgatg aaaacagact
     4741 cagcagcgga cgcagcagaa aaccgtcgag gcgcagggtt tgcatcctag ccatatagtg
     4801 tgaacgactg gcctccccca tacgttcgcc aaatcgcgcc tgctgacgaa actgctggat
     4861 aacgctcatg ccattgatga tttcgttaaa gccgtcgttg atatccgcca gataggcgcg
     4921 cacacggcgg acaatcggcg tgctgtaacg ctggtatatc accattacca ccagcaccac
     4981 cgggaaaatc attatcgcca ccagcgccat tcgccagtcg aggctgaaca tcgccaccag
     5041 catcgcgccc accagtgcgg cactgcgcag gacagttgcc actacggtaa cgtagagatc
     5101 gcggatcact tcagtgtcat tagtgacgcg ggaaatcacc tgcccgacgg gttgggtatc
     5161 aaactcgctt aatggctggc gtaacgcagc atccatcacg tcggtacgca actgttgcac
     5221 tgcgcctact gccgcccgat taaacagcag cgactgcgcg taatgtagcc cggcggcaaa
     5281 cagttgtagc ccaacatacg ccgcagccag ccctgcaacc actttcaacg gcaggttatt
     5341 tttcgctacc atattgtcga taaaatagct gataagcagc ggcccactga cttctgccgc
     5401 cgccgcaacc cacatcatca ggaccgcaat ccccagcggt ttacgccacg gcgaaccata
     5461 cgctaacagg cgcttgagag tcggccacag ttggctaaaa ctacgcatcg acggcctcct
     5521 cgcgaatttc cggaacgtcg tcgagcgccg cctccagttg ttgatagcga tacatatcgc
     5581 gataccaacc gctttgttgc gccagctcat catgattgcc acgctgggcg atatgtccgt
     5641 gctgcatcac aataatttca ctggcttccg tcagtgccga aaggcgatgg gcactgatga
     5701 ttaccgttct tccctgtccc cactgacgca ggttatgcag gatctggtgc tctgtgcgtc
     5761 cgtccaccgc cgaaagcgca tcatcaagga tgaggatttc cgcgttgact aataacgctc
     5821 gagcaatgga gatacgctgt ttttgcccac cggaaagcat cacgccgcgc tcgcccactt
     5881 ctgtatcgta accttgcggt agacgcaaaa tatcgtcatg tacgctggct aaccgcgcga
     5941 catgctcaat ctcttgctgg gtggcattcg ggcaacccag cgcgatgtta ttcgccacgg
     6001 tgtcagaaaa aaggaatggc gtctggctga ccaccgccag gcggctacgc cagctatcga
     6061 gttgtaactt cgtcagagga atatcatgaa agcgaatatc cccctcgctg acgtcgaaat
     6121 gacgctgaat gagcgacaac agggtacttt tgccggaacc agtcggcccg caaataccca
     6181 gcatctgacc gggtttcagg gcgaaattga cgttttccag cgcaggatgg tcagtctgcg
     6241 gatacgtgaa ctggtgaata tttacatcca gttcgccacg cccttccggc acgggttcgc
     6301 taccatcgtt caccaccggc gcttccgcca gcatggcgcg aatacggctg tatgcagcac
     6361 taccacgttc cacaatgtta aacatccatg ccagcgccag cattggccaa atcatcagac
     6421 ctaaatacat cataaaactg gtgagctggc ccagcgttaa actgccctgc accaccatcc
     6481 aactaccgcc gccaatcgcc agcaagttcg ccataccaat cgcgatatag atggtcgggt
     6541 cgaagcgagc atcaatacgc gccacccgca tgtttttttt gccggtatct tcggcatccg
     6601 cggcaaataa cgccgactgg cgatcttcca gaccaaaggc tttgatcatg cggatactgg
     6661 tgaggctttc ctgggtgcgg tcattaagac tggaaaacgc cgcctgtgcc agcttaaagc
     6721 gttcatgcaa ggcatcgccg ttgcgcttaa tcatgatcgc catcactggc atcggtaaca
     6781 gggcaaataa ggtcaactgc cagctaattt gcgtagacat cataatcaac acagcgcagc
     6841 ccattaccag tgaatccacc agcgtcagca ccccttctcc ggcggcaaac acgacgcgat
     6901 cgacgtcatt ggtcgcacga gccatgagat cacccgtgcg atgacgcagg taaaactccg
     6961 ggtgctgccg gcttagctga cggtaataat cttcacgcag ttcaacagcc agttggtaag
     7021 acgcgccaaa cagcaatacg cgccagacat aacgcaggag ataaaccaca acggcaatca
     7081 gcaccatggt ggcgatccac atcaggctca gcccggtagt aaagtgttgt tctgtcacgc
     7141 catcgacaac aataccaacc acttttggcg gaaccagttg cagcatcgcg ataatgacaa
     7201 gcaaggcgac agccccaaga tagcgacgcc attcccgacg gaaataccag cttaattgag
     7261 caaataatcg cacgcgttgt gtcctgacct gattctggat atttattcga tgggtaaaga
     7321 agtggtgtat ttaatctgtt ccatcgcgaa gctggaagtg acgtccgaca gccccggcac
     7381 gctgtttacc agacgcttat aaaactcgtc gtagcgtttc atgtcggcaa cctggacgcg
     7441 catcagataa tcgtattcac cagccatgcg ccagaccccc agcacttctg gcatttcggt
     7501 aaccaccgtg acaaagcggc aataccattc gctgctgtga tgttgcgttt ttatcagcac
     7561 aaaggcggtc aggccgaggc ctattttttc cggatccagc agggcgactt tgccgataag
     7621 gataccgtcg tcctccagcc gtttcaggcg cttccagcag ggggtggttg tcagattaac
     7681 ggcttcagcc agtgcctgca aagagagggt gcaatcctgc tgcagtaagg ccagcagctt
     7741 acggtcaatt ttatctaaca tagcccttcc acggagaatt tttttctctt ttaattctat
     7801 tttaaaggtg aaatcgcgac aatttattct gtgcggttta aacaccacgg cacaaaatga
     7861 caaattcaac gcaaaaaaaa accgggcaat tgcccggtgc tggaagggat ctcgttattc
     7921 gggggaataa ggtagatgtg gatagtccag ccagtgcgtc aaatagtgag agacagcctg
     7981 atttcggcaa tgtccaatca ccggtaaatg cgggagctcc gcgcgcagtt gcggcatcgc
     8041 attgcccata ataaacccgc taccgacgct gcctaacatt tcgcgatcgt tcatcgcatc
     8101 accaaaggcc atgcaatcgc gcaacgataa acctaaatgt tgggtccgca ccgtcaatgc
     8161 agcgccttta ttgcagccca ccggcagcac ttcgaggcaa tccgtggcgg aaaaacacaa
     8221 atgtgcacgc tcgcctaatg cttcgtatag ctggatctgc aagcgtgtaa gatcgtcgtg
     8281 atcgccacag aagcagatct tggtgacgct gccgagtggc atttttttga catcgattat
     8341 ctgataacga aaaccgctat agacaaatac ctgcaacaac gcagggctct ctttcccggt
     8401 aaaccaaccg tcgtcattga agatatgcat gctggctcgg gtatcccatt gctgatacag
     8461 caccagctcc gcgacatccg caggtaaatc atcacgatgt aaaagttcac cttccagaga
     8521 atgcacgcgc gttccgttgc cggtaatcaa atatgcatcc agcgatagcg ccccgagaat
     8581 atgctgcatc tccagcgcat gacgccccgt ggcaaaagtg agggtaatgt cgcgttcacg
     8641 cagtcgcgcc aaagtagaga gggttttctc acctaaatga tggtcaggca tcaataaagt
     8701 gccatccata tcaaatgcta ccagacgagc catttctttc tccactctgt gacacggtta
     8761 atggtgattg agtatcacct gatatatacg gaagtaatag tgaatagtta aataatattg
     8821 ttccgggttt atatgcgatt gctcaaccgt cttaaccagt atcaacgtct gtggcaacct
     8881 tcagccgcaa accgtcaccg tcagcgaact ggccgaacgc tgtttttgca gcgaacgcca
     8941 tgttcgtacg ctgttgcgtc aggcacagga ggcgggatgg ctggagtggc aggcgcagtc
     9001 aggacgcgga aagcgcggac aattacgctt tctggtcacg ccggaatcgc tacgcaatgc
     9061 gatgatggaa caggcactgg aaaccggaaa gcagcaagat gtgctggagc tggcgcaact
     9121 ggccccaggt gagctgcgca ctctgttaca gccatttatg ggcggacaat ggcaaaacga
     9181 tacacccacg ttgcgtattc cctactatcg cccgctcgaa ccgctacaac caggcttttt
     9241 gcccggccgt gccgagcagc atctcgccgg gcagatattt tccggcctga cccgcttcga
     9301 taataatact cagcgcccga ttggcgattt agcgcatcac tgggaaacct ctactgacag
     9361 gttacgctgg gacttttatc ttcgttcaac cctacactgg cataacggcg atgcagtaaa
     9421 agcctcacac ttacaccagc gattattgat gctgttacaa ctgccagcac tggatcaatt
     9481 atttattagc gtgaagcgta ttgaagtcac ccatccgcag tgtctgacct tctttttaca
     9541 tcgccccgat tactggcttg cgcaccggct ggcgagctat tgcagccatc tggcgcatcc
     9601 gcaattccca ctgatcggca ctggtccttt tcgcttaaca caattcacag cggagctggt
     9661 gcgcctggag agccatgatt attaccattt gcgtcatccg ctgcttaaag cggttgagta
     9721 ctggataact ccgccgcttt tcgaaaaaga tttgggaacc agttgtcggc atcccgtgca
     9781 aatcaccatc ggcaaaccgg aggagctgca acgggtcagc caagtcagta gtggcatcag
     9841 tttaggtttt tgctatttaa cgttgcgcaa aagtccccga ctctccctct ggcaggcgcg
     9901 aaaagtgatc tccattattc atcaatccgg tttattacaa acgttagaag tcggagaaaa
     9961 cctgatcacc gccagtcatg cattactgcc aggctggact attccgcact ggcaagtacc
    10021 ggatgaagtc aaactaccga aaaccttgac gctggtttat cacctaccga tagaacttca
    10081 taccatggca gaacgcctac aggcgacact ggcagcggaa ggctgtgaac tcacaattat
    10141 ttttcataac gcaaaaaact gggacgacac gaccctactg gcacacgcag acctcatgat
    10201 gggcgacaga ttaattggcg aagcaccgga atatactctg gagcaatggc tacgttgcga
    10261 tccactatgg ccacatgttt tcgacgctcc agcatatgca catctgcaat cgacgctgga
    10321 cgcggtgcaa gtaatgcctg atgaggaaaa ccgatttaat gccctgaaag cggtttttag
    10381 ccagttaatg gcagacgcga cgctgacgcc gctgttcaac tatcactatc gcattagtgc
    10441 ccctcccggc gtgaacggtg tgcgactgac accgcgcggc tggtttgaat ttaccgaagc
    10501 ctggcttccc gcgccgtcgc aatgaatagc tggtccgggt taatcgcagg cgttaccata
    10561 acgcttttat tgttcaagca ggattatcta tgaaacgtgc tgtcgttgtg ttcagtggag
    10621 gccaggattc caccacctgt ctggtgcagg cattacaaca atatgatgaa gtccattgcg
    10681 tgacgttcga ttacggtcag cgacatcgcg cagaaatcga cgtggcacgc gaactggcgc
    10741 tgaaactggg agcacgcgcg cataaggttc ttgatgtaac gctgctcaac gagctggcgg
    10801 tcagcagcct gacacgtgac agcattccgg tgcctgatta tgaacctgaa gccgatggca
    10861 tcccgaatac gtttgtccca gggcgtaata ttttgttcct gacgctggcg gcaatatatg
    10921 cgtatcaggt aaaagcagaa gccgtaatta ccggcgtctg tgaaacggat ttctccggct
    10981 acccggattg ccgcgatgag tttgtgaaag cactaaacca tgccgtcagt ttgggcatgg
    11041 cgaaagatat tcgttttgaa acgccgctga tgtggattga taaagcggaa acctgggcgc
    11101 tggcagatta ttacggcaaa ctggatttag tccgtaacga aacgttgacc tgctataacg
    11161 gcattaaagg cgacggttgc ggtcattgtg cggcatgtaa tttacgcgcc aacggtttga
    11221 atcattatct ggccgataaa ccgacggtga tggcagcgat gaagcagaaa accgggttga
    11281 ggtaattatt ccgggtgtcg ccggatgcgg cttgagcatc cggcaccaca aaacgtttac
    11341 ttaaccatct gctccagctt ttcgcgcaat tccccttcca gagctaatgc tttctgcgtt
    11401 ttaagatcaa tacaaacaaa cgtaataagc gcatccgcta ccacctgccc ttccggctcc
    11461 agtgtaatga cctggtttaa gatgccgctt ttaccgttta attgctgcaa ctgactggta
    11521 atagttaaca ggtcgcttaa taccgctgga cgacgatagt taatattgat attgaccacg
    11581 acgaaggcga tgttatgggc cgtcatccac tgaaaactgt cgctattttc caccccatcc
    11641 cagcgggctt cttcgagaaa ttcaaggtag cgggcgttgt tgacgtgctg gtaaacgtcg
    11701 agatgatatc cacgaacttt gatttgtgtt tgcatagcgc aataacctta cgttgttgtt
    11761 ataagtacag aggtcataac tggtatgacc tcttcagtct ggcaaaaaat tgccactatg
    11821 caaattaatt acagggttaa taccgccaga ttacgttcca ccagcgaatt gcccatcccc
    11881 ggcacctgct ttagatcctc caccgtttta aacggaccgt actcttcgcg ataactgaca
    11941 atcgcctgcg ctttcttcag gccaacgcca ttcatcgcgc gggctagctc ttccgcgctg
    12001 gcattattaa tgctgacccg ggtgccttct tcgtcactgg ctttcgccgg tactgctgct
    12061 ttactttgtg ctgcaggagc ttccgctttg gtttctaccg ccgtcggttt cgccacagaa
    12121 gctgccgcca gcgcgctatg agacattccg gcacaggcca gggacagtgt aatgagcagt
    12181 gctttaattc cgtgtttcat actgttttct ccttgtttgt taacagtgga atcaccttaa
    12241 cgggagtgaa aacagtaaac aaatggcaat gttcagaaat ggaaaaggcc gcgaaagcga
    12301 ccttttcttg ttacagctgc aacgcatttt tttgcgaggc ggcttcagga ttattgctgt
    12361 tccagcgcat cgccaatttt gattttcgcc tctttacgca ggttactcat cagagcttca
    12421 aagacgattt gtgcgttgtt ctgggtgata ccctgcacca tcgctttttt ctgatcttcc
    12481 ggcattgaac cttgtttcac ttcatccagc gccagcagaa ccacattacc ttgcatatcg
    12541 gtcgccatac cgtagctcgg tttgtctttc gctggcagtg gcagtgcaaa cgccgcctgg
    12601 ctaatcgggt cacgaccgga acggcttaag gttttcggct cgccaaattt cagaccggca
    12661 gcctgcatag cttccgcacc tttgccggct ttcaaatcaa ccagcagttt ctcagcatcc
    12721 actttcgcct gttgttcagc tttgttgtgc tgaaccagtg ccttaacttg ttcctgaaca
    12781 tctgccaacg gtttcaccgc ttccggtttg tgctcgctga cgcgcagcac gaatgcgcgg
    12841 tcgccgtcta cggtgatgat gtcagagttg atgcccgacg cgccgttttc acctaccaga
    12901 ccgccgttaa agatagcgtc ggcaaccggc ttgaagttca actcttccgg caggttatct
    12961 ttgctgaacc aacccgtctg agtggcttta acgccggcag cttgctctgc accggccaga
    13021 gactcggtgt cgttgcttgc cacatcgctc actttctgct gcagcgcata gtacgcatcg
    13081 agggcttttt cgtgtttcac tttcgccgca atgtcgtcac gtacttcgtc taacgatttc
    13141 actttcgctg gctgaatgtc gtccagacgt acaatcagga aaccgaccga agatttgatg
    13201 acaccagaca gttggccttt ttctttcaga ccagcatttt tcagttcgtc ggggatagtg
    13261 gcatcttcta accaacccat atcgccgccg ttacgggcag agataatatc ggcagatttt
    13321 tctttggcta atgcagcaaa atcaccgcct ttattcagct catcaagtac cgctttcgct
    13381 tcatcttcag ttttggtctg gatgatgctg tagcgggtac gctgcggctg ggtgaattga
    13441 tcctgatgct gatcgtagta gctctggata tccgcatcgc taaccggttg ctgcatcgtt
    13501 gcggcaacca gcttgatgta actcacgcgg aattgttccg gcgtcatgaa attgtttttg
    13561 ttttgttcgt agtagctggc aatctcctgt tcggtcacag gctgcttcgc cgccagcgcg
    13621 ttaacatcga tagtcgcctc acgcaccacg cgttgttgcg cgaccagtgc cgccagctcg
    13681 tcggtttcac ctttcagcat aaaatcggta ccggcaacgc cgttaatcag ctgttgggta
    13741 gtgagctggt tacgcagcgc ctgggcgtac tgatcggcgg tcatccccat ctggttgagg
    13801 ataccgttat agcggctgtt atcaaatttg ccgtcaacct ggaaggctgg ggtcgcgaaa
    13861 atcgcctgtt taacctgctc atcactgata cccagtttca gctcacgagc gtactgatcc
    13921 aacagcgcct cgtcgatcag acgattcagc acctgttgac gcagggtttt catatagcct
    13981 tcgttcgctg ccagctcgga gtattgatcg cccagctgtt gctgcatacg attacgctcg
    14041 ctgttaaagg cgttctcgaa ctgcccacgg ctgatttcct ggtcattcac ttttgcggcg
    14101 tagttattgc ctccgccaat caggtaacca ctcacgccgg tcaatatgaa cgacacgata
    14161 atgataccga aaataatctt gagcacgaga ctgtttgcag ccgtgcgtaa gctgtccatc
    14221 atggtgtaac aacactccgc tgtagatgac tgtgtacctc acgcaacgta tcgatgcgcg
    14281 caaaggccta ttgtgacaag aaacgggggc aattgtcagc ccacaagaca cagaaagtca
    14341 ccgaatacaa ataaaaaagg cacatcagta gatgcgccct tgaacttcgt cacatcccca
    14401 ctggggacaa cgcttagttt accgcgtctt tcagtgcttt acctgcacgg aagctcggta
    14461 ctttagcggc agcgatggtg atctctttac cggtctgcgg gttgcggcca gtacgggcag
    14521 cacgctcttt aacggcaaaa gtaccaaaac ctaccagtgc tacatcatcc ccttctttca
    14581 gagattcagt tacggaagca ataatagcat ctaacgcacg gccagccgca gctttagaga
    14641 tatcagcccc tgcagcaatc ttgtcgatca attgagattt attcactctt ctcttcctct
    14701 ttataattta tatcgcactt gaatccttca aggtacgaac gcgcagcagt tatatcaggc
    14761 cagccatccc cttacaacac cccttaataa gggcaagccc gatccgctat ctaacttagc
    14821 gagacaaaaa aaggctggca agcccgaatt agcctgccag ccctgttttt attagtgcat
    14881 tttgcgcgag gtcactattt tgcagtcaca acctgcatgc cagacggttc attttgcagc
    14941 gccagagtca gaacttcctc aatgcgcttc acaggatgaa tgtccagatc ggcaattacg
    15001 ttgtcaggaa tctcttccag atcgcgttta ttttcgaacg gaattagcac tgttttaatc
    15061 ccgccgcgat gcgctgccag gagtttttct ttcaaaccac cgatcggcag tacctgacca
    15121 cgcagagtga tctcaccggt cattgccaca tcggcacgaa ccgggttacc ggtcaggcaa
    15181 gaaaccagcg cggtgcacat agcaataccg gcactcggac catctttcgg cgtcgcacct
    15241 tccggtacgt ggacgtggat gtcacgtttt tcgtaaaaat cagggttgat ccccagtttt
    15301 tccgcacgcg cacgaaccac cgttaacgcc gcctgaatag actcctgcat cacttcgccg
    15361 agcgaaccgg tataggtcag tttgccttta cccggaacac atgcggtttc aatggtcagc
    15421 aagtcaccgc ccacttccgt ccacgccaga ccgattacct gaccgacacg gttttcgtta
    15481 tcagcgcgac catagtcgaa acgctgaaca ccgaggtagt catgcaggtt atcgccgtta
    15541 atttcgatat gttttaatga cttatcgagc agtaactgct taaccgcttt gcggcacagt
    15601 ttggagattt cacgctccag accacgcacg cccgcctcac gggtgtagta acgaataatg
    15661 ccgataatgg cgctatcgtc gacggtcagc tcaccttttt tcagtgcatt acgttcaatc
    15721 tgcttcggca gcaggtgacg tttggcgatg ttcagttttt catcttcggt ataaccggag
    15781 aggcgaatca cttccatacg atccagcagc ggtgccggaa tgttcatgga gttcgacgtc
    15841 gcgacaaaca tcacgtcgct gagatcgtaa tccacttcca ggtagtggtc gctgaacgct
    15901 acgttctgct ctggatccag cacttcaagc agtgcagagg ccggatcgcc tcgcatgtca
    15961 gaagacattt tgtcgatctc atcgagcagg aacagcggat ttttcacgcc cactttcgcc
    16021 attttctgga tcagtttacc cggcatagaa ccgatgtaag tacggcggtg accacggatt
    16081 tccgcttcat cacgcacgcc gcccagcgcc atacggacat atttacgccc ggtggctttg
    16141 gcaatggact gaccaagaga ggttttacct acccccggcg gccctaccag gcagaggatc
    16201 ggtcccttga ttttgttgac acggctttga accgcaaggt actcaaggat gcgatctttc
    16261 acgcgctcca gaccataatg gtcggtatca aggatttcct gcgcctgacg caggtctttt
    16321 ttgaccttgc tgcgcgcatt ccacggtacc tgtaccatcc agtcgatata accacgcact
    16381 acggtcgctt ctgccgacat cggagacatc attttcagct tctgcaactc tgcttccgct
    16441 ttctcttttg cctctttcgg catcttcgcc gcgtcgattt tgcgcttcag ggcttcgttt
    16501 tcgtccggca cgtcgtccat ttcaccgagt tctttctgaa tagctttcat ttgctcgttc
    16561 agatagtact cacgctggga tttctccatc tgctttttaa cgcggttgcg aatgcgtttc
    16621 tcaacctgca gcagatcgat ttccgattcc atcattgcca tcagatattc cagacgttcg
    16681 ttaacgtcgg acatctccag aacggactgt ttgtcagcca gtttcagcgg catatgtgca
    16741 gcaatggtat ccgccagacg cgccggatcg tcgatgctat tcagcgacgt cagcacttct
    16801 ggtgggattt ttttgttcag cttgatgtag ccttcgaact ggctgattgc agtacgcacc
    16861 agcacttcct gttcccgctc atcaatggtc ggcgactcca gatactccgc cttcgcagaa
    16921 aagtgttcgc cattgtcaga gagcgcagaa atacgcgcgc gctgtaaccc ctcgaccagc
    16981 actttgacgg tgccgtcagg cagtttcagc atctgcaata tagaggccac ggtcccgacg
    17041 gtgaaaagat cgtttacacc cggctcatcc gttgaagctt ctttctgcgc gaccagcata
    17101 atttttttat catggtccat cgccgcttcc agacaacgga tagatttttc ccgcccgaca
    17161 aataagggga tgaccatgtg cggataaacc accacatcgc gcagcggcaa tacggggatt
    17221 tcaatgcgtt cagaacgctc aggattcata gagctctctc ttagtttaat ttccgccagg
    17281 taatcagatg acacgactgt gcttcacgcc atctattaac atgtacgtca gtatatgggg
    17341 atgtttcccc cacattcaac gccgagaata gaggaaaaat taaaggggag ataaaatccc
    17401 ccctttttgg ttaactaatt gtatgggaat ggttaattat tcaccagatg cctgttgcgc
    17461 ttccggctta ccataaatca gcaacggttt gctttgacca tcaattaccg actcgtcgat
    17521 aaccactttt tcgacgtctt ccatggacgg cagatcgtac atggtatcga gcagtgcggc
    17581 ttctacgatg gaacgcaggc cacgggcacc ggttttacgc gccatcgctt tcttagcgat
    17641 agcatccagc gcctcgtcac ggaattccag atccacgcct tccagattaa acagcgcctg
    17701 atactgcttg gtcagggcgt ttttcggctc tttgaggatc tgaatcagag cttcttcgct
    17761 cagttcattc aacgttgcta caaccggcag acgaccaatg aactcaggga taagaccaaa
    17821 cttgatcaga tcttccggtt caacctgcgc cagcagctcg ccttcgcttg ctttgtcgga
    17881 cttcgctttt accgtcgcgc caaaaccaat gccggagccg gtttctacac ggtgggaaat
    17941 cactttatcc agaccggcaa acgcaccgcc acaaataaac aggatcttag aggtatcaac
    18001 ctgcaagaat tcctgctgcg gatgtttacg cccaccttgc ggtggaacag cagctaccgt
    18061 accttcgatc agtttcaaca gtgcctgctg tactccttca ccggaaacgt ctcgggtaat
    18121 ggacgggttg tctgacttac gagaaatctt gtcgatttca tcgatgtaga caatcccgcg
    18181 ctgcgctttc tgtacgtcgt aatcgcactt ttgcaacagc ttctgaatga tgttttcaac
    18241 gtcttcgccc acataaccgg cttcggtcag cgtggttgcg tcggccatgg tgaacgggac
    18301 gtccagcagg cgcgccagcg tttcagccag cagcgtttta ccggaaccgg tcggaccgat
    18361 cagcagaatg ttacttttgc ccaactcgac accattgctg gtatcgccgt tgcgcagacg
    18421 tttgtaatgg ttgtataccg cgaccgccag cacttttttc gcctgttcct ggccgataac
    18481 gtaatcgtcc aggtggttgc gaatttcatg cggcgtcggt agcgcactgc gttcacgatg
    18541 cggtgcaact tctttaatct cttcgcgaat gatgtcgtta cataaatcaa cacattcgtc
    18601 gcagatatac acggatggac cggcaatcag cttgcgcact tcatgctggc ttttgccgca
    18661 aaaagagcaa tacagcaatt tgcctgagcc atctttgcgt ttatctgtca tgggtcaaaa
    18721 cctcttcttt gttctttgtg ccgcacacga cgacgcaaat gccattctca ggcgcaagcc
    18781 gcttacagcg ttgtgccgcc ctagataagt atagcggcac agttgcgcct ctggcatcaa
    18841 ttacgatggg tcagaatcga atcgaccaga ccgtattcca ccgcttcagg ggcggaaagg
    18901 aagcgatcgc gctcggtatc acgttcaatc tgttctaatg attgacccgt atgaagcgcc
    18961 ataagttcat tcatgcgccc tttaactttc agaatttcac gggcatgaat ttcgatatcg
    19021 gtcgcctggc cctggtagcc gcccaacggt tggtgaatca tcacgcgcga attcggcagg
    19081 caaaaacgtt taccttttgc ccctgcggtc aacaagaaag cgcccatcga ggccgcctgg
    19141 cccatacaga tggtgctgac atcaggcttg ataaactgca tggtgtcata gatagacatc
    19201 ccggcagtga tcaccccgcc tggggagtta atgtacagat agatatcttt ttctgggttt
    19261 tccgcttcca ggaacagcat ctgcgccaca atcaggttag ccatgtggtc ttcaacctgg
    19321 ccagtcagaa aaatgacgcg ttccttaagt agacgagaat agatatcaaa agagcgctca
    19381 ccgcgtgagg tctgttcaat gaccatcggc accagcgcca tatggggtgc aaagttatct
    19441 cgttcgccgc tgtatgacat ttccgtctcc tggataaaat tgaaaaaaac ctgctgtacc
    19501 gattgtaacc ttatggacgg attatcagct agtccctgtg ttatgggtac ggcatcacca
    19561 tatttcaagc ataacaatct tttgttgtta cgctaacacc gaaagaggct tttcgcacgg
    19621 ctccatgcaa aattcatgac aaaaaaaagc ccaccacctg gcggtgacgg gcctttgtgc
    19681 gaatttagcg cgttatgctg cgtaaattac gcctgctggt tcatcagctc gttgaaagtg
    19741 gtttcttttt cagtcacttt cgctttcgcc agtacagctt caacagcctg ttcttccaga
    19801 gcaacattgc gcatgttgtc catcagttct ttgtttttgc tgtagaactc gataacttct
    19861 ttcggatctt cgtacgcaga agccatctct tcgatcaggc ctttcacgcg ctcttcgtca
    19921 gctttcagct cgttggtgcg gataacttcg cccagcagca ggccaacaac tacgcggcgt
    19981 ttagcctgtt cttcgaacag ttcgcgcggc agttccagag cttgtttttc gttgccaccg
    20041 aaacgctgtg cagcctggcg acgcagaacg tcgatttcgc tgtcgatcag cgcagccggt
    20101 acgtcgatgt cgttagcttt taccagacct tcgatcgcct gagacttaac gcggttacgg
    20161 atggcgctct tcagctcgcg ctccatgttt ttacgcactt cagcgcgcag accttctacg
    20221 gaaccatctt caacgccgaa acgtttgatg aattctgcag tcagttccgg cagttcacgc
    20281 tcttcaactt tcttcaggtt gatagcgaat ttcgctgctt tacctttcag gttttctgcg
    20341 tggtattctt ccgggaaggt cacgtcgatg gtgaactctt cgccagcttt gtggcctttg
    20401 ataccgtctt caaagcccgg gatcatacga ccctggccca tcgccagtac gaaatcagac
    20461 gctttaccgc cttcgaactc ttcgccgtct acagaaccgg tgaagtcgat ggttacgcgg
    20521 tcttctgctt caacagcgcc gtctttttct ttccaggtcg cctgctgttt acgcagagta
    20581 tccagcatgc cgtcaacgtc agcgtcggtc acttcaacga tcggtttttc aacttcgatc
    20641 gcttccagac cctgcagttc aacttccgga taaacttcaa actctacaga gtaagtgaag
    20701 tcttcaccca gcttgtattc gcccggaaca taagtcggtg cgccagccgg attgattttt
    20761 tctttaatga tggcgtcaat gaagttacgg ctcatcaggt cacccagaac gtcctggcgt
    20821 acagacgcgc cataacgctg agcaacgata ttcattggca ctttgccttt gcggaagccg
    20881 tcaatacgta cttttttcgc aacgttgacc agctcgcttt taacagcggt ctcgatgctg
    20941 tcagcagcga tagtaatcgt tacacggcgg ccaaggcctt gagtggtttc aactgaaact
    21001 tgcatcttgt tacctcaaaa aatcacagtg ctcggtcaac tcttaccccg caagcacagg
    21061 ttgttggctt cgcggaaccg ggatgttctt ataatcaatc acattccctg ttaccagaat
    21121 catcccgaaa gcattcaaat aggacgcggc attatagcgg catcactttt atgagtcgag
    21181 aacggttatt gcgcgctgtc gcgtattttt tcacaattcc cgggcaattt tcgtctgaat
    21241 gctgggcaaa aagcgcaaaa tccagacaaa acaaaacggc ccgcaggccg tttttcataa
    21301 aatctttagc aacatactgg aaaagcgccg acagttgcaa atgcgttttt acgcgatgct
    21361 tcctgctcca cgacagggag gagaggcaaa gacggtgtcc tgcaaccctt cccactcctt
    21421 aatagtgtaa gtatgcagag ccagcgcatg aacggtagta gagagttcct ccgctaaagt
    21481 actgtaaatc attcgatgac gattcagaaa acgttcaccc gtaaaacgat cgctgaccag
    21541 cacaacttta aaatggcttt cagagccggc tgggacattg tgacgatagc tttcatccac
    21601 tacttcgagg aatacgggtt ggaacgccgc ccttaatttt tcttctatcc gctcacgtat
    21661 catcatgaaa tatctcctcc gacaacgctg agatgtcatc catcccttta aatactagcc
    21721 gcttttaccg tttccattgc agcttaacaa caaatattta ggtttcgtcc tggtttttcc
    21781 gtcaagcgta tgaagtttac tgatttaaag attgaaacac aggcctcgca tcagcgtttc
    21841 ttttaccagg ggtgattatt tcgtcaacat gatgaattta catgccttac ccacttcccc
    21901 tcgccgttgg caatgttatg atggcgggaa tttttaccaa acacgcttaa gagtctctga
    21961 gagcccgaac atgttcaaaa aaatcctctt cccgttagtt gctctgttta tgcttgcagg
    22021 atgcgcaaaa ccgccaacaa ctattgaagt ttccccgacg attacactgc cacagcagga
    22081 tccaagcctg atgggcgtca ccgtaagcat taatggtgcc gatcagcgta ccgatcaggc
    22141 gctggcaaaa gtcacccgcg ataatcaaat cgttaccctg accgcctccc gcgctctgcg
    22201 tttcctgctg caagaagtgc tggaaaaaca gatgaccgcg cgtggttaca tggttggtcc
    22261 gaatggcccg gttaatctgc aaatcatcgt tagccaactg tatgctgacg tgtcccaggg
    22321 caacgtgcgc tacaacatcg ctaccaaagc ggatatcgcc atcatcgcta ccgcgcagaa
    22381 tggcaacaaa atgaccaaaa actatcgtgc cagctacaac gttgaaggtg cgttccaggc
    22441 ctccaacaaa aatatcgccg atgcggttaa cagtgtgctg accgatacca tcgctgatat
    22501 gtctcaggac accagcattc acgaattcat caagcagaac gcgcgttaat ttctgccctc
    22561 tggcccggtg caagccgggc ctgtagacgc ccatgtccag tcaatattta cgtatttttc
    22621 aacagccgcg ttcagccata ttgctgatcc tgggttttgc ttccgggcta ccgctcgccc
    22681 tgacatccgg caccttacag gcctggatga cggtcgaaaa tatcgatctc aaaaccattg
    22741 gtttcttctc tctggtaggc caggcttacg tttttaaatt cctctggtca ccgctgatgg
    22801 accgctacac gcccccattt tttgggcggc ggcgcggttg gctgctcgcc acgcaaatcc
    22861 tgttattagt cgccattgcg gcgatgggtt ttctcgaacc aggcacccaa ctccgctgga
    22921 tggcggcgct ggcggtggtg atcgcttttt gctctgcctc ccaggatatt gtcttcgatg
    22981 cgtggaaaac cgatgtgctc ccggcagaag aacgtggtgc gggcgcggca atcagcgtgc
    23041 tgggttaccg tttagggatg ctggtttccg gcggcctagc cctgtggctg gcagataaat
    23101 ggttgggctg gcagggcatg tactggttga tggcggcgct gttgatcccc tgtattatcg
    23161 cgacgttgct tgcaccagaa ccaaccgaca ccattcctgt gcccaaaacg ctggaacaag
    23221 cggttgttgc acctctgcga gatttctttg gtcgcaataa tgcctggctt attttgcttc
    23281 ttatcgtgct gtataagctg ggcgacgcat tcgccatgag cctgacaacc acgtttttga
    23341 ttcgcggcgt cgggtttgat gcgggtgaag taggcgtggt aaacaaaacg cttggcttac
    23401 tggcgaccat tgttggcgca ttgtacggtg ggattttgat gcagcgcctg tcgctgttcc
    23461 gggcgctgct gattttcggc attttacaag gtgcgtctaa cgctggttac tggctgctgt
    23521 cgattactga taagcatctc tacagcatgg gcgcagccgt ctttttcgaa aacctctgtg
    23581 gcgggatggg cacatcagcc tttgtcgcgc tgttaatgac gctgtgtaat aagtcatttt
    23641 ccgctactca atttgccctg ctctcagcgc tttccgctgt agggcgagtt tatgtcggcc
    23701 ccgtggcggg ttggtttgtt gaagcacacg gctggtcgac attctatcta ttctccgtcg
    23761 ccgctgccgt accggggctt attttgctgc tggtttgccg ccagacgctt gaatatacac
    23821 gagtaaatga caactttatc tcccgtaccg aatatccggc aggttatgcc tttgccatgt
    23881 ggacactggc ggcgggcgtc agcctgttgg ccgtgtggtt acttctgttg acgatggacg
    23941 cgctggattt gacgcacttc tctttcctgc ctgctctgct ggaagtcggg gttttagtcg
    24001 ccctttctgg cgtcgtgctt ggtggtttgc tggattatct ggcgctacga aaaacgcatc
    24061 tgacgtaatc tgtaaatatt atttagctgt agtaatcact cgccgtaatt attacggcta
    24121 aataaacatc agcatcgctt attaatattt gttcttttgt gcggcttagc gtttggaaat
    24181 tattgacgcc atttataaat tctatttttc ttacacgatt cagctaatga gtctttattt
    24241 tctcaccacc cagttgtcac tttaatgata attatttgtt aaataattgt tttatttcgc
    24301 attggttata ccaattgccc accaagactc cttctgcact ccccttttgt ta
//
LOCUS       DAZPAB010000043        24281 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZPAB010000043 DAZPAB010000000
VERSION     DAZPAB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.043"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..1101)
                     /locus_tag="ACHUPG_001530"
     CDS             complement(<1..1101)
                     /locus_tag="ACHUPG_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR0861194.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGAL"
     gene            complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="ACHUPG_001531"
     CDS             complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="ACHUPG_001531"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR0861195.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="ACHUPG_001532"
     CDS             complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="ACHUPG_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR0861196.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="ACHUPG_001533"
     CDS             complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="ACHUPG_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR0861197.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="ACHUPG_001534"
     CDS             complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="ACHUPG_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR0861198.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="ACHUPG_001535"
     CDS             complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="ACHUPG_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR0861199.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3405..4382
                     /gene="pspF"
                     /locus_tag="ACHUPG_001536"
     CDS             3405..4382
                     /gene="pspF"
                     /locus_tag="ACHUPG_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR0861200.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="ACHUPG_001537"
     CDS             complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="ACHUPG_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFR0861201.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="ACHUPG_001538"
     CDS             complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="ACHUPG_001538"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR0861202.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="ACHUPG_001539"
     CDS             complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="ACHUPG_001539"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFR0861203.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="ACHUPG_001540"
     CDS             complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="ACHUPG_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR0861204.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="ACHUPG_001541"
     CDS             complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="ACHUPG_001541"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR0861205.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10284..11702
                     /gene="puuA"
                     /locus_tag="ACHUPG_001542"
     CDS             10284..11702
                     /gene="puuA"
                     /locus_tag="ACHUPG_001542"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR0861206.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11851..12015
                     /locus_tag="ACHUPG_001543"
     CDS             11851..12015
                     /locus_tag="ACHUPG_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861207.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12005..13390
                     /gene="puuP"
                     /locus_tag="ACHUPG_001544"
     CDS             12005..13390
                     /gene="puuP"
                     /locus_tag="ACHUPG_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR0861208.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13524..13769
                     /locus_tag="ACHUPG_001545"
     CDS             13524..13769
                     /locus_tag="ACHUPG_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR0861209.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14082..15725
                     /gene="sapA"
                     /locus_tag="ACHUPG_001546"
     CDS             14082..15725
                     /gene="sapA"
                     /locus_tag="ACHUPG_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR0861210.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15722..16687
                     /gene="sapB"
                     /locus_tag="ACHUPG_001547"
     CDS             15722..16687
                     /gene="sapB"
                     /locus_tag="ACHUPG_001547"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR0861211.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16674..17564
                     /gene="sapC"
                     /locus_tag="ACHUPG_001548"
     CDS             16674..17564
                     /gene="sapC"
                     /locus_tag="ACHUPG_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR0861212.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17564..18556
                     /gene="sapD"
                     /locus_tag="ACHUPG_001549"
     CDS             17564..18556
                     /gene="sapD"
                     /locus_tag="ACHUPG_001549"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR0861213.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18558..19364
                     /gene="sapF"
                     /locus_tag="ACHUPG_001550"
     CDS             18558..19364
                     /gene="sapF"
                     /locus_tag="ACHUPG_001550"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR0861214.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19432..19785
                     /locus_tag="ACHUPG_001551"
     CDS             19432..19785
                     /locus_tag="ACHUPG_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR0861215.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20153..20941
                     /gene="fabI"
                     /locus_tag="ACHUPG_001552"
     CDS             20153..20941
                     /gene="fabI"
                     /locus_tag="ACHUPG_001552"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR0861216.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21085..22212
                     /locus_tag="ACHUPG_001553"
     CDS             21085..22212
                     /locus_tag="ACHUPG_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFR0861217.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22280..24214
                     /gene="rnb"
                     /locus_tag="ACHUPG_001554"
     CDS             22280..24214
                     /gene="rnb"
                     /locus_tag="ACHUPG_001554"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR0861218.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
BASE COUNT         5527 a         6394 c         6305 g         6055 t
ORIGIN      
        1 taatgcacct tcctgctgta atgcctcgac cagcgctaat atttcgcttt ctggaagcaa
       61 gccacgcagt gggttcaacc cgatgccatc gtgagaggcg aggaagttaa accaggtcgt
      121 tttactggag ggcaagctca gactttgcgc ccactggcac agcgcctcga cgttctgttt
      181 ttgcaccgca tgtaacacca gcggcggcag cgagaactgg tacaccatat gcgcttcgtc
      241 atcaccgttg ccaaagtaag caatgttgtc tttgtgcgga acattggttt cggtaataat
      301 cactgtacct ggtgctacgt catcaataat cgaccgtaac agcttgataa tcagatgtgt
      361 tttttccaga tggatgcagc ttgttcccgg ctctttccac ataaagccaa cggcatccag
      421 gcggacatac tcagcacctt tctcaaggta acagagtaaa acatccacca tcgccagcaa
      481 cacttcaggg ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg tccataaatg
      541 gcgcgtggaa ttatcatcca tttgaaacgg cgttaatagc ggtaacgcac gcggaagggt
      601 gacggcactg agatcggttt tcggatcaac cgcaataaag aaatcttcaa aacccggaag
      661 ctgttgtaaa tagtttttaa accattcact ttttgcagac atatggttgc agacaaaatc
      721 aaacattaaa tgactgcatt caccgagttg ctgaatatcc tgccactccc ccgcttcact
      781 ggcgacctga tgataatcaa ttaccgaaaa tccatcatca gatgaccatg ggtaaaaagg
      841 taataaatga acatgtgaaa aaatgctttg cagccattga tggtagaact gattaaatat
      901 gggtagtggt tttaaatcat tactgtgaaa ttgatcggca taggtaatta acacgacatc
      961 actttcatcc cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac tctcaagacg
     1021 cgttacaagt ttctgtaaat gagttgcggt aaatgttcca ccgtagattt cgtccatgta
     1081 atccgtaatt ttctgtttca tgttgccttt ccgtggtagc ggtcccatga ggcgagagac
     1141 tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc aggaaatcat
     1201 tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatag ggatgtaaat
     1261 aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca tggtgaattc ttttaacctt
     1321 tgaccttcgg cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg gtatatccca
     1381 tctcgctaag gagctctttt gcctgcccgg actggcgtcc ggcattgcaa tacactttca
     1441 cggtgtcgtt tttatccgga acggcggtgg caatgcgctc tttcacttcc ttcaggggaa
     1501 tattgatggc cccctgaacg tgctcttgct gatactgctc tggaacacga acatcgatcc
     1561 agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt aagccttttt
     1621 ttaaacatgt aagcgtcctt ctatggatga aaattgccat ccattatacg cctgagattt
     1681 aacaattttt gcgaaactcc cctcaccttt tataacgctg tgccagttta ttagcagccc
     1741 gacttaacag cggttccagc gccacagcca gcaacatttt cagcggtcgg cgagcaactg
     1801 attttatcgc ccagcccgcc acacccgccg ggccatagcg cagtgcggta agaagtacca
     1861 gcttgcctgc taatttgaaa ccaagcttca ccttttgccc ggcctgttgc cagcgagtat
     1921 tcataacttt cctcacagtt gacggaaacg gctacgtaac gtgaaagtat cggaagtgac
     1981 ataacgttcc atctcgcgta aacgcgtttc acttgccgcc agttcacggt cgacttcatc
     2041 cagcaattcg ctgctggaag gtagctgctc accaaaggcc atgttgtccg gcattggatc
     2101 aagcgcaaat gacaaaatga tgtaagcaac cagggtaaac agcgccagac cgaaaaaaat
     2161 cgacagcacc accaggatac gcaccagttt taccggtaca tcaaaatagt tggcaatccc
     2221 ggcgcagacg ccgcggacca tgccctgctg tggaatacgc cataactttt tattgagatt
     2281 aatgcccgcc attagcgatc cctccagttc ggatgttctg catcaagaat agattccagc
     2341 gcctgaatac gttcgcgcat ccgttttgct tcatcaacca gttgcgctaa tcgctgctgc
     2401 tcactttgcg acaattcact gcgaccagaa cgattgctgt aatgcagcca taaccagatc
     2461 ggtaaaacaa acagcacaaa aatggttaac ggaatagcca gaaatagcgc gctcataagt
     2521 actccttaga cgatgagcgg cggcgcgttc ggacgccgcc tggatgttat tattgattgt
     2581 cttgcttcat tttggctttt aattgtgcca gttgttcgct gattgcatca tcggctttca
     2641 gttcggcaaa ctgatcgtcc agcgattttt gtttaccgaa gctgtggctt tctgcttccg
     2701 cttccatctg gtcaatacga cgttcgaaag attcgaaacg agccattgct tcatccagtt
     2761 tgccactgtc cagctgacga cgcacatcgc gcgacgagtt tgccgcctga tgacgtaaca
     2821 tcaatgcctg ctggcgagcg cgtgtttcgc tcaatttgtt ttccagctcg ccaatctctt
     2881 tcttcatgcg tgccagcgta tcgtccacca gcgtcacttc atgttccagg gacttaatca
     2941 gatcggtcag tttctgtttt tcaattaacg ctgcacgtgc cagatcctct ctctctttca
     3001 gcagcgccag ttcggctttt tcctgccatt caacttcacg cgccgacgct tgttcaatac
     3061 ggcgagtcag ctgtttcttt tctgccaacg cacgcgccga agtagaacgt acttcaacca
     3121 gtgtatcttc catctcctgg atcatcagac gcaccagttt ctgtggatct tccgctttct
     3181 ctaacagagc gttgatgttg gcattcacga tgtcggcaaa gcgagaaaaa atacccataa
     3241 tgttgtcctc ttgatttctg cgttcaggat tgtcctgctg aactgttaat acaatttgcg
     3301 tgccaatttt ttatcttttt gatttataaa gatctgattg aagaatcaac agcaacatgc
     3361 caggatgaat tagcgaatta cactaacaag tggcgaattt catcatggcc gaatacaaag
     3421 ataatttact tggtgaggcg aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg
     3481 caccgctgga caaaccggtg ctcatcatcg gcgaacgcgg caccggtaaa gagttgattg
     3541 ccagccgcct gcattatctc tcctcccgtt ggcaagggcc gtttatttcc cttaactgcg
     3601 cggcgttaaa tgaaaatctg ctggattccg aactgtttgg tcacgaagcg ggggcgttta
     3661 ccggtgcgca aaaacgtcat cctgggagat ttgaacgtgc cgacggcggt acgctatttc
     3721 ttgatgaact cgctacggca ccgatgatgg tgcaggaaaa attattgcgt gtgattgagt
     3781 acggtgaact ggagcgcgtt ggcggtagcc agccgttgca ggtgaatgtg cggttggtat
     3841 gcgcgacgaa tgccgatctc ccggcgatgg tcaatgaagg tacttttcgc gctgacctgc
     3901 tcgaccggct ggctttcgat gtagtgcaac tgccgcccct gcgcgagcgc gaaagcgaca
     3961 ttatgctgat ggcagaacac tttgccattc agatgtgtcg ggaaatcaag ctgcctctgt
     4021 tcccggggtt tacggagcgc gccagagaaa cattgctgaa ttatcgttgg ccgggaaata
     4081 ttcgtgaatt gaaaaacgtg gtggaacgtt cagtgtatcg ccacggcacc agcgattatc
     4141 cgcttgatga catcattatt gatcccttta aacggcgtcc acctgaagaa gccatcgccg
     4201 tttcagaaaa tacctcgctt ccgacgctgc cgctggattt acgcgagttt cagatgcagc
     4261 aggaaaaaga gttgctgcaa ctcagtttgc aacaggggaa atataaccag aagcgcgcgg
     4321 ctgaattact ggggttaact tatcatcagt tccgcgcgtt gttgaaaaag caccagattt
     4381 agcgaagttg agcaatacaa cttgccggat gcggcgtgaa tgctttattc ggcatacata
     4441 atcaatacgt cgtttgcagg cctgataagc gcagcgcatc agacattatt gcgttgggct
     4501 attaatcgct cagcgcatcc tgcaaaattt tcattgccgc atcgaattgc gcatccggga
     4561 tggtcagcgg atacaggaag cgaatcacgt tgccgtatgc gccacaggtc agcaggagca
     4621 gcccctgcgc cagcgcgcgt tgctggattt tctgtgcaat cgccgctgac ggctcgcccg
     4681 tttgcggatc gttaaactct gccgcaatca tcgaccccag gccgcgtacc gcagcaatgg
     4741 ccggaacgct ttctttggca tcaatcaacg tgtttttgag acgctggccc agttgattcg
     4801 cgcgttcgca gagtgattct ttgtcgataa tattgagcac cgcgtgcgcg gcagccaccg
     4861 ccagcgggtt cccggcgtag gtgccaccca acccgcccgg cgcgggcgcg tccataatat
     4921 tcgcgttacc gaccacgccc gaaagcggca tcccgcccgc gaggcttttc gccatcgtca
     4981 ttaagtccgg cttatcggcg taatgatcca tggcaaacag tttaccggta cgcgcaaagc
     5041 cgctttgcac ttcatcggca atcatcacaa taccgtgctc gtcgcacagg cggcgaatgg
     5101 cggcaaccag ctcttttggc gcaacgttga aaccgcactc gccctgcacc ggttcgaaaa
     5161 taatggccgc gacctgcttc gcttcgatgt ctgatttaaa caagcgttcg atggcgtcga
     5221 gggagtcctg tgttgaaatg ccgtgtaaat ctgacggata aggtacgtga tacaccgaac
     5281 cagggaacgg gccgaagccg attttgtacg gcgcaacttt tccggtcagc gccatggtca
     5341 tatatgtacg accgtggaag ccaccgctaa acgcaatcac gccaggtcgt ccggtatggg
     5401 cgcgagcaat tttcaccgcg ttttccaccg cttccgcgcc ggtagtgaag aatgcggttt
     5461 tagcctgccc gctcactggg gcaagggtgt taattttctc cgccagcgtg acgtagcttt
     5521 cgtacggcac aatctgatac gccgtgtggg taaactgttg caattgctgc tccaccgccg
     5581 cgaccagatc agggtggcga tgcccggtat tcagcaccgc aatgcctgcg gcgaaatcga
     5641 tgtattcgtt gccctctacg tccttcagcg tagcgttttc tgccgactgg gcgaagaagt
     5701 tacacatcac gccaaccccg cgtggggtag cagaaagacg acgctgatgg aattcattgt
     5761 tgctcatgtt tcacaccttt tgactcttca ctgttaatcg gaaattaaac gcccagacga
     5821 tcgcgcaggc tgtaataagc cgcgcccatc gcggtaaatg gcacgcgcag cgtgcgcccg
     5881 ccggggaacg ggtaatgcgg cagattggcg aaggcatcga aacgctcggc gtcaccgcgc
     5941 agcagttcag caatcaaacg cccggcaaga tgggtacagg tgacgccgtg gccgctgtaa
     6001 ccctgcatgt aatagatgtt ggtatcaagg cggccgaact gcggcatgtg cgacaaggtc
     6061 agcaggaagt tgcccgtcca gcggtaatca attttcacgc ccttcaactg agggaaggtt
     6121 tttagtagtt tcggtaccac aagacgctca acgtcatccg ggttgcgtgc accatacact
     6181 acacccccgc cgtacagcag gcggttgtcg gcggtaagac ggtagtaatc aagcagatag
     6241 ttacagtctt ccacacagta gtttttcggg atcagcgaac gggctaaatc ttccgacagc
     6301 cgttcggtgg tgatcacctg cgtgccgcac ggcatgctgc gtttcgctag ttccggctct
     6361 actttatcgc ccagatacgc attcccggcg acaatcacat acttcgccgt cacctgacca
     6421 ttggcagtcc gaaccacggc tggcgtggtg tgctggatct gcgtcacggc ggaaagctca
     6481 tacacgcgcc cgccgttcag gcggatggca tcggcttcac caatggcgag gtttagcggg
     6541 tgaatatgcc caccgctgtg atccagcagc gcaccagtgt agcgatcgct ggcgacctcg
     6601 cggcgaatag cgttgacgtc cagcagctcc agctgtttat tgccgtagcg tacccagttc
     6661 tctttctgct cttcgagtgt ggcgagttgt ttatcattca tcgccacaaa cagaccgccg
     6721 gggcggtagt cgcagtcaat ctgataacgt ttgatacgtt cgcggatgat ctcgccaccc
     6781 tcgaacatca tgctgccgag catacgggcg gtgtccatgc cgtagctttt ttcgatcacg
     6841 tcgatgtcgc ggctgtagga gttcacaagc tgcccgccat tgcgcccgct tgcgccaaag
     6901 ccgatgcgtg aggcttcgag aaccactacg tcaaagcccg cttccgccag atgcagcgcg
     6961 gaggagagcc cggtatagcc gccgccaacc acgcaaacgt cgcaggtgat tgactcattc
     7021 agtgtgtcga atggtgcata tttattcgca ctggcggcgt agtaactgct ggtatgttcg
     7081 gtcatttcag gcctccaggc ttatccagat ggttttcagt tcagtgaatt tttcaagggc
     7141 atgcagggat ttgtcgcgac cgttgccgct ctgcttatag ccgccaaacg gcacggtcat
     7201 atcgccgtcg ttgtagttat tgacgaagac ggaaccggct ttcaggcgtc ggctcatgcg
     7261 gtgcgcgcgg gagaggtcgc gcgtccatac cgccgcgcca aggccgtact ggctgtcgtt
     7321 ggcaagctgc agcgcctgat cttctgatgt gaaacgggtg accaccagca ccggaccgaa
     7381 aatctcttcg cggcttaagg acgcattcgg gtccacatcc acgaagatgg tcgggccgat
     7441 ggccgctgcc agcccggcgt tgcgaccatc cagcagcagt tgccccttgc cttcgccttc
     7501 acgaataaag gtgtgtaccg agtcagcgtg agcgctgtcg attaaggtgc ccatggtggt
     7561 tgcgggatca agtggatggc ccggctgcca gttttgcgcc tgctgtttta acagggctaa
     7621 gaattcatcg gcgatgctct cttccagtaa caaacgcgtt ccggcgatgc gcacctggcc
     7681 ctggttgtag aagatgcctg cggcggtggc gcttgccgct ttttgcagat ccggacaatc
     7741 ggcgaaaacg atgttggcgc ttttgccgcc cgcttccagc cagacacgtt tcatgttgct
     7801 atcacccgcg tctttcagca gttgtttacc cgtacgcgtg gagccggtaa aggcgatggc
     7861 atcgatatcg ttatgacgcg aaagcgcctg ccccgcttca tgaccaaaac ccgtcaccac
     7921 gttcaacaca ccatccggca agcctgcttc tttcgccagc cccgcgagac gaatcgcact
     7981 gagcggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca gcgccgggcc
     8041 gagtttccag caagtcagca acagcgggaa gttccacggc acgatggcgg caatcacgcc
     8101 gaccggttca cgcacgatca tcgccagctc atggctactg gtggtcgcca cttcgccata
     8161 cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg gaatatcatc
     8221 acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg ccagctcttc
     8281 ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt tagccggaga
     8341 agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg cacggtcgat
     8401 atcgacgctc ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg gatcaacggt
     8461 ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata agcggttttc
     8521 aatggcgaga cttaacgctt tatcctgcca gtaagccaga tgatgaaaat tcattatgac
     8581 tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca gagatccccg
     8641 ggctgcatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg cattgcgctt
     8701 acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct gataattcgg
     8761 caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat ggcataactt
     8821 tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc accttccagt
     8881 actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt gcccggctgg
     8941 tacgtttcaa agatcatcgc cagcgtgcga ttcgggttac cgttatgaac cagcttcatt
     9001 gacacaccct gactgcccat ctcaattaag tcgtcctgat taatgacgac ctgcggctca
     9061 tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac cttcagcagc
     9121 ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact gatagcactg
     9181 tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt
     9241 tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt tccttaaact
     9301 gtagtggtca gagtcgctgt ttttcagcga tatggtgctg ccaagcggtg ataaagccct
     9361 cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc cactgtacgc
     9421 ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc ggcgagcgag
     9481 cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga gagtttaccc
     9541 aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca acctgaactt
     9601 catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga tgttccagca
     9661 attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc accagttctt
     9721 gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca ttaattatcg
     9781 ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt tcaccatata
     9841 ggtgcggctg cacattgctg ggactactag gaagataaat gccatcgagt ttcggcaaaa
     9901 gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc aagccgcctg
     9961 cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga cccttaagcc
    10021 tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc atgctcaatc
    10081 tcacaaaatg gactaaatta tcgccattac tgcgctttaa atgaccttta tgttcaatat
    10141 tttttcaatc tagcagtgga ttttcatttt tgcaaactca atttaacatt tgacaaacat
    10201 ttggtttgca tacagattcg aatgatggtc attatatttt acgctttgat aacgagcgga
    10261 aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag aactttgttc
    10321 agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc tacctggagc
    10381 gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt tgcttccgtg
    10441 gaaaacgtat ccctgtttca agcctgaaga agctcgagaa agggtgttat ttcccggcct
    10501 cggtatttgc aatggatatt ctgggcaacg tagtagaaga agcaggtctg ggtcaggaaa
    10561 tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc cttaactcct tctgccgccg
    10621 atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc gctccctttg
    10681 acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag cgcggattgt
    10741 tcccggttgt agcggtagag ctggagttct atttactgga tcgccagcgc gacgctgaag
    10801 gggatctgca accgccctgc gcgcctggca ccgatgaccg caatacacaa agccaggttt
    10861 actccgttga taacctcaac cacttcgccg acgtgctcaa tgatattgat gaactggcgc
    10921 agttgcagct gattccggca gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa
    10981 tcaaccttta ccatactgat aacgtgctgg aagcctgcga tgatgcgctg gcactaaaac
    11041 gcctagtgcg tctgatggca gaaaagcata agatgcacgc cacttttatg gcgaagccgt
    11101 atgaagagca cgcgggcagc gggatgcata tccatatcag tatgcaaaat aatcgtggcg
    11161 agaatgtgct ttctgacaca gaaggcgaag attcgccgct gctgaaaaag atgctcgccg
    11221 ggatgattga cctgatgccg tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc
    11281 gccgcttcca gccgggaatg tatgtgccga cgcaggcgtc gtggggccat aacaaccgca
    11341 ccgtcgccct gcgtattccg tgcggtgacc gtcataatca ccgcgtggaa tatcacgtgg
    11401 cgggtgccga tgccaaccca tatctggtga tggcagcgat ttttgccggt attttgcatg
    11461 gccttgataa cgagctgccg ttgcaggaag aagtcgaagg caacgggctg gaacaggaag
    11521 gcttaccctt cccgattcgc cagagcgatg ccctgggtga gtttatcgag aatgatcacc
    11581 tgcgccgcta tttaggcgaa cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc
    11641 tgttgcagtt tgagcgcctc atcacagaaa cagaaattga gtggatgttg aaaaacgcgt
    11701 aattcccctt gcggggttgt ttcatcggcc tgagcggagc caggcctgac atttgctggt
    11761 aatccagaca tttcccggat gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa
    11821 cgattaagac tgtgacgagg aaatgagatg atgccgatga ttaaatcccc gcatggcgag
    11881 ggaggctgcg tatgcgcccc tcctgctact gactggactc cgcccccttt gcttcccttg
    11941 ctaaacaggt ttgatttcag gtcgacacga ccgcaaacct tattacgcag gggaggcagc
    12001 aattatggct attaattcac cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg
    12061 aaaatcactg aaattgtggc aggtggtaat gatgggtctg gcctatctca cgccgatgac
    12121 cgtatttgat acttttggta ttgtctccgg cattagcgac ggtcacgttc cggcgtccta
    12181 tttgctggcg ctggcgggcg tgctgtttac cgctatcagc tacggcaaac tggttcgcca
    12241 gtttccggag gccggttcgg cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg
    12301 atttatggtc ggctggtcat cactgctgga ttatctcttt ttgccaatga tcaacgtttt
    12361 gttggcgaaa atctatctct ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt
    12421 aaccttcgtc gccattttaa ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt
    12481 caataccctg tttgtactgg tgcaaatctc catcatggtg gtgtttatct tcctggtggt
    12541 tcagggactg cataaaggag aaggcgttgg caccgtctgg tcacttcagc cgtttatcag
    12601 cgagaacgcg cacctgatcc cgattattac cggtgcgacg attgtctgtt tctcgttcct
    12661 cggtttcgat gcggtgacca cgctttcgga agagacgccg gacgccgcac gcgtgatccc
    12721 gaaagccatc ttcctgacgg cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt
    12781 tatgcagctg ttctttcccg atatcagccg ctttaaagac ccggacgccg cactgcctga
    12841 aattgcgctg tacgtcggcg gcaagctctt tcagtcgatt ttcctctgca ccacgtttgt
    12901 gaacacatta gcgtctggcc tggcttcaca tgccagcgtg tcgcgtctgc tgtatgtgat
    12961 ggggcgtgac aatgtgtttc cggagcgcgt gtttggctat gtgcacccaa aatggcggac
    13021 tccggcactc aacgtcatta tggtcgggat tgtcgcgctg tcggcgctgt tctttgattt
    13081 agtcaccgcg acagcactga ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct
    13141 gtcggtgttt aatcatttct ggcggcgtaa aggaatgaat aaaagctgga aggatcactt
    13201 ccactatttg ctgatgccgc tggttggcgc gctgacggtg ggtgtgctgt gggttaacct
    13261 agagtcaacg tcactgacac tcggtctggt atgggcttcg ttaggcggcg catatttgtg
    13321 gtatctgatc cgccgctatc gcaaagtgcc gctgtacgaa ggtgacagaa cgccggtgag
    13381 cgaaacgtaa tagccggatg cgcggcgcat tatcctgcct gagggtaacg cgccgcgcca
    13441 ttaactataa aaagatgtat gacccggatc acgagtgcta tcatccgccc ttatgcttag
    13501 cgcattaacc ccaggggcaa ataatgaacc acgatatccc gctaaaatat tttgatattg
    13561 ccgatgagta cgcgaccgaa tgcgcagagc ctgtcgcaga ggctgaacgc acaccgctgg
    13621 cccactactt ccagttactg ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag
    13681 cccaacatga aatggctgct gaagcgggga ttaatcccgt acgcattgat gagatcgcag
    13741 agtttttgaa tcaatggggt aatgagtaat tatttaattg cagacgatcg gtcagtatcg
    13801 ttgtccggat aaagcgttca cgccgcatcc ggctaacaat gacacgcact gcagcgtgct
    13861 ttaatgggtt tctcacgatc gtaccgctct gctcctcgct aagttgttct gtgagttata
    13921 gttatagaga aatcacgggt acgaatgttg aacagatggc gacgccacct gttcaacgac
    13981 caaccacacg gccctcatta cgacccctga acacaaacgg taatggcaaa tgtcattaaa
    14041 tattagtaag atagcctctt tacataaaat cccttaatat tatgcgccag gtattatcgt
    14101 ctcttttggt gattgctgga cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc
    14161 cgcatgctga tatccgcgac agcggttttg tctattgcgt cagcgggcaa gtcaacacct
    14221 ttaacccatc caaagcgagc agtgggttaa ttgtcgatac ccttgccgcc cagttttatg
    14281 atcgactgct ggatgtcgat ccctatacct atcgcctgat gccggaactt gccgaaagct
    14341 gggaagtact cgacaacggc gcgacctatc gcttccacct gcgtcgcgat gttccgtttc
    14401 aaaaaaccga ctggtttact cccactcgta aaatgaatgc cgacgatgtg gtgtttacct
    14461 tccagcgaat ttttgaccgc aacaacccgt ggcataacgt caacggcagc aacttcccct
    14521 acttcgacag cctgcaattt gccgataacg tgaaaagcgt ccgcaaactg gataatcata
    14581 ccgttgagtt ccgactcgcc cagccggatg cttctttttt gtggcacctc gcaacccatt
    14641 atgcttcggt catgtcggca gaatatgccc ggaagttaga gaaagaagat cgccaggagc
    14701 aactcgaccg tcaaccggtc ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc
    14761 aatttattcg cctacaacgt catgatgact tctggcgcgg taaaccgtta atgccgcagg
    14821 tagtggtgga tttaggctcc ggcggcaccg gacgtctgtc gaaactcctg accggggaat
    14881 gcgacgttct ggcctggcct gctgccagcc agctatccat tttgcgtgac gacccgcgct
    14941 tgcgtttaac gctgcgtcct gggatgaacg tcgcctatct ggcatttaac accgccaaac
    15001 cgccgctaaa taatcccgct gtccgccatg cactggcact ggcgattaat aaccagcgcc
    15061 tgatgcaatc catctattat ggtacggctg aaacggcggc ctctatttta ccgcgcgcct
    15121 cgtgggccta tgacaacgag gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac
    15181 agttgaaggc gttggggctg gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc
    15241 aggcgtggaa ccccagtcca ctgaaaactg ccgaactgat tcaggcggat atggcgcagg
    15301 ttggcgtaaa agtggtgatt gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg
    15361 atatgagcca tgatctgacg ttatccggtt gggcgacgga cagtaacgac ccggacagtt
    15421 tcttccgtcc gttactgagc tgcgcggcaa ttcattcaca gaccaacctc gcccactggt
    15481 gcaatccgaa attcgacagc gtgttgcgta aggcgctctc ctcgcagcag ctggcagcgc
    15541 gtattgaagc ctatgacgaa gcgcagagta ttctggcgca ggaattacct attctgccgc
    15601 tggcgtcgtc attgcgtttg caggcctatc ggtacgatat caaaggtctg gtacttagcc
    15661 cgtttggtaa cgcctccttt gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac
    15721 catgattatc ttcaccttac gccgcatttt gttattgatt gtcaccttgt tcctgctgac
    15781 cttcgttggc ttcagcttga gctatttcac tccacacgcg ccgttacaag gcgcgtcact
    15841 gtggaatgcc tgggtgttct ggtttaatgg cctgatccac tgggattttg gtgtttccag
    15901 cattaacggt caaccaattg ccgaacagtt aaaagagctc ttcccggcga cgatagaact
    15961 gtgcatcctc gccttcggtt ttgcgctgat tgttggcatc cccgtgggga tgattgccgg
    16021 gattacgcgc cataagtggc aggataactt gatcaacgcc atcgccttac tgggcttttc
    16081 aatcccggtg ttctggctgg cgcttctgtt gacactgttt tgttcactca cgctaggttg
    16141 gttgcccgtt tcagggcgtt tcgatctgct ctacgaagtg aaaccgatta ccggttttgc
    16201 gttgattgat gcctggcttt cggactcacc atggcgggat gaaatgatca tgagcgcaat
    16261 ccgccatatg atattgcccg tgatcaccct gtcggttgcg ccaacaactg aagtgatccg
    16321 cctgatgcgt atcagcacta ttgaagtgta tgaccagaac tatgtaaaag cggcggcgac
    16381 ccgcggtttg tcgcgcttta ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc
    16441 ggttattcct cgcctgggct tacagttttc caccatgctg acgctggcga tgatcaccga
    16501 aatggttttt agctggccgg gtttagggcg ctggttaatt aacgccatcc gccagcagga
    16561 ttacgcagcc atttccgccg gagtgatggt gtgtggctca ctggtcatta ttgttaacgt
    16621 gatttctgat attttgggtg ccatggctaa ccctctgaaa cataaggaat ggtatgcctt
    16681 acgatagcgt atacagcgaa aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat
    16741 tttatagtga cgcctctgcg atggtcggcc tgtacggctg cgcgggactg gctgtactgt
    16801 gtatttttgg cggctggttt gcgccttacg gcatcgacca gcaatttctc ggttatcaat
    16861 tattgccgcc atcatggtcg cgctatggcg aagtttcttt cttcctgggg actgacgacc
    16921 tcggacgtga tgttttaagc cgtttactga gcggagctgc gccaaccgtg ggtggcgcat
    16981 ttgtggtgac gcttgccgcg acgatctgtg gcctggtact gggcaccttc gccggggcga
    17041 cacacggcct ccgctcagcg gtgcttaacc atattctgga taccttactg gcgatccctt
    17101 cgctgctgtt ggcaattatc gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt
    17161 ttgctgtctg gctggcattg ctgccgcgta tggtacgttc gatttacagc atggtgcatg
    17221 acgaactgga aaaagagtac gttatcgccg cccgtctgga tggcgcatca acgctgaata
    17281 ttctctggtt tgctgtgatg ccaaacatca ccgccgggct ggtcaccgag atcacccgcg
    17341 cactgtcgat ggcaattctc gatatcgccg cgctgggctt tctcgatctc ggcgcacaac
    17401 tcccctcgcc tgaatgggga gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc
    17461 cgtggactgt catgctgcca ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc
    17521 ttggcgatgg cgttcgtcgt gcgattattg cgggggtgga ataatgccat tactcgatat
    17581 tcgtaacctg accattgaat ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt
    17641 aagtatgacc ttaaccgaag gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa
    17701 gagtttgatt gcgaaagcga tttgtggggt gaataaagat aactggcgtg ttactgctga
    17761 ccgtatgcgt tttgatgata tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact
    17821 ggttggccat aacgtgtcga tgattttcca ggaaccgcag tcgtgtcttg acccttcaga
    17881 acgtgtgggt cgccagttga tgcaaaacat cccagcctgg acctataaag gccgttggtg
    17941 gcagcgtttt ggctggcgca aacgccgtgc cattgaactg ctgcaccgcg tggggattaa
    18001 agatcacaaa gatgcgatgc gcagttttcc ctatgagttg accgaaggtg aatgtcagaa
    18061 agtgatgata gccattgcac tggcgaatca accgcgtctg ctgattgctg acgaaccgac
    18121 aaactcaatg gagccaacaa cccaggcgca aatctttcgc ctgctgacgc gtctcaacca
    18181 aaacagcaat accactattt tgcttatcag ccatgactta caaatgctta gccaatgggc
    18241 ggataaaatt aacgtgcttt actgcggtca aacagtggaa accgcgccga gtaaggagtt
    18301 ggtgacgatg ccacatcatc cttataccca ggcgctgatc cgcgcgatac cagacttcgg
    18361 cagcgcgatg ccgcataaaa gtcgcctcaa tactctaccc ggcgctatcc cattgctgga
    18421 acagttaccg attggttgtc gtctggggcc acgttgcccg tatgcacaac gagaatgcat
    18481 tgtgacgcca cgtttgacgg gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa
    18541 catggagaaa gagtgagatg atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt
    18601 tccgctaccg gaccggttgg tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct
    18661 ttacgctacg tgaaggccag acactggcga ttattggcga gaatggttcg ggtaaatcca
    18721 cactggcaaa aatgctggcg ggaatgatag aacctaccag cggcgagtta ttgattgacg
    18781 atcatccgct gcattttggc gattattcct tccgtagtca gcgtattcgc atgatttttc
    18841 aggatccttc gacctcgttg aatccccgtc aacgtatctc acaaattctg gattttccac
    18901 tgcgcctgaa caccgacctg gaaccggaac agcgtcgcaa acagattatt gaaacgatgc
    18961 gtatggttgg gctgctaccg gatcacgtca gttactatcc gcatatgttg gcacccggac
    19021 aaaaacagcg tctgggtctg gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg
    19081 atgaagccct cgcttcactg gatatgtcaa tgcgttcgca gttgattaat ctgatgctgg
    19141 agttacagga aaaacagggc atttcgtata tttatgttac ccagcatatc ggaatgatga
    19201 agcacattag cgaccaggtg ctggtgatgc atcagggcga ggttgtcgag cgaggcagca
    19261 ccgcggatgt gctggcatcg ccgctgcatg aactcaccaa acggctgatt gccgggcact
    19321 ttggcgaagc attgacagcg gatgcgtggc gtaaagatcg ctaattttct gaatcctcct
    19381 tccagattat tcacctcatt ctgacaagag attaaatctt gtcagaatga ggtgatggat
    19441 aaaataaaat caaatgcgcg tgatttaaga cgcaatctca ctttgcagga acgaaagctc
    19501 tggcgatatc ttcgcagccg acgttttggt gatttcaaat ttcgccgtca acatccggtg
    19561 gggagctaca ttctcgattt tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt
    19621 gggcagcatg atttggcagt tgcctatgat accaggcgca ctagctggct tgagtcgcag
    19681 ggctggaccg tgctgcgttt ctggaataac gagattgatt gtaatgagga ggcggtgctg
    19741 gagattattc tgcaggaact gaaccgccgg tcaccctcac cctgaaaggg cgagggggcc
    19801 gaccgagccg aatagctgtt gtggtgaaaa catggagaca gtgctggaga atattcggca
    19861 aggtctgaac catcccagcc atcgccataa aagggttagg ggctgtatga gcctgtttgt
    19921 tgctggggta acaatatttg cacaatatgg tcccctcgcc cctctgggga gagggttagg
    19981 gtaaggggaa aagcgccccc cctgccgcag cctgctccgg tcggacctgg taactatagc
    20041 tactcacagc caggttgatt ataataaccg tttatctgtt cgtactgttt actaaaacga
    20101 cgaatcgcct gattttcagg cacaacaagc atcaacaata aggattaaag ctatgggttt
    20161 tctttccggt aagcgcattc tggtaaccgg tgttgccagc aaactgtcca tcgcctacgg
    20221 tatcgctcag gcgatgcacc gcgaaggagc tgaactggca ttcacctacc agaacgacaa
    20281 actgaaaggc cgcgtagaag aatttgccgc tcaattgggt tctgacatcg ttctgcagtg
    20341 cgatgttgca gaagatgcca gcatcgacac catgttcgct gaactgggga aagtttggcc
    20401 gaaatttgac ggtttcgtac actctattgg ttttgcacct ggcgatcagc tggatggtga
    20461 ctatgttaac gccgttaccc gtgaaggctt caaaattgcc cacgacatca gctcctacag
    20521 cttcgttgca atggcaaaag cttgccgctc catgctgaat ccgggttctg ccctgctgac
    20581 cctttcctac cttggcgctg agcgcgctat cccgaactac aacgttatgg gtctggcaaa
    20641 agcgtctctg gaagcgaacg tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg
    20701 tgttaacgcc atctctgctg gtccgatccg tactctggcg gcctccggta tcaaagactt
    20761 ccgcaaaatg ctggctcatt gcgaagccgt taccccgatt cgccgtacag ttactattga
    20821 agatgtgggt aactctgcgg cattcctgtg ctccgatctc tctgccggta tctccggtga
    20881 agtagtccac gttgacggcg gtttcagcat cgctgcaatg aacgaactcg aactgaaata
    20941 atcgttctgt tggtaaagat gggcggcgtt ctgccgcccg ttatctctgt tatacctttc
    21001 tgatatttgt tatcgccgat ccgtctttct ccccttcccg ccttgcgtca ggataacgat
    21061 ttcctttacg accaaggagc gcccatggaa caacgccaca tcaccggcaa aagccactgg
    21121 tatcatgaaa cgcaatccag tactacggag tatgacgttc tgcctctggt cccggaagcc
    21181 gcaaaggtca gcgatccctt tctgctcgac gtgatccttg atgaagagac gctggccccc
    21241 ttcctttcat ggctggtccc tgcgcgcgtt cttgcagtgg agttgttccc tgaccagctt
    21301 accgtgaccc gttcacaaac cttcaccgct tatgaacgct tgtcgacggc cctgacggtt
    21361 gctcaggttt gcggcgtcca gcggttatgt aactactatt cggcgcgact tacgccgctc
    21421 cccggacctg attcctccag ggaaagtaat catcggttgg cacaaatcac gcaatatgcc
    21481 cgccaactgg ctagctcgcc ttctattatc gacaaccgat cgcgccagca tctgaatgac
    21541 gtcggtctta ctgtctggga ctgtgtgatc attaaccaaa tcattggttt tattggcttt
    21601 caggcgcgga caattgcgac atttcaggct tatctcgggc atccggtacg ctggttaccc
    21661 gggctggcga tacaaaacta cgccgacgcg tcactgtttg ctgatgaatc aatacgctgg
    21721 cgaagcagct atgaggtgga aaaactacct gaagagcaca caaaaagttc aactgcagaa
    21781 ctttgccaac tggccgaaac actctctctc caccctattt cactttccct tctcgaaaag
    21841 ttgttaaaca gcacacgggt caatgcacag ccggataatc agcttgcggc gttgttatgc
    21901 gcgcgtataa atggcagtcc tgcttgtttt tccacctgta tggattcatc aaatgaatat
    21961 aaaaaaatca gcacccttct gcgcaagggc gaaaatgaaa ttaaccgatg ggctgaccgt
    22021 cattctgttg agcgcgctac cgttcaggcg atacaatggc tgacccgagc acccgatcgc
    22081 tttagcgccg cccagttcag ccctttactc gaacacgaaa aatcatcaac gcagattatt
    22141 aatctgctgg tatggagcgg gctgtgtggc tggataaatc gcttaaaaat cgcgttgggt
    22201 gagacatatt aaccttgccg cgtcagacag attcgcgtaa aactgtcagc cgctctaatg
    22261 gccaccaaaa tagacaatta tgtttcagga caacccgctg ctagcgcagc ttaaacagca
    22321 actgcattcc cagacgccac gcgctgaagg ggtggtaaaa gccacagaaa aaggctttgg
    22381 cttcctggaa gtcgacgcac aaaaaagtta tttcattccg ccgccgcaga tgaaaaaagt
    22441 catgcatggc gaccgaatta tcgcggtgat ccacagtgaa aaagaacgtg aatccgcaga
    22501 gccagaagaa ctggttgaac cgttcctgac tcgtttcgtg ggtaaagttc agggcaaaaa
    22561 tgaccgtctg accatcgttc ctgatcatcc acttttaaaa gacgccattc cttgccgcgc
    22621 agcccgtggc ctgaaccacg agtttaaaga aggcgactgg gcggttgccg aaatgcgccg
    22681 tcatccgctg aaaggcgatc gttctttcta tgctgaactg acacaataca tcacttttgg
    22741 tgacgaccac tttgtaccgt ggtgggttac ccttgcgcgc cataatctgg aaaaagaagc
    22801 accagacggc gtcgctaccg aaatgctcga tgaaggtctg gttcgtgaag atctgacctc
    22861 gctggatttt gtcaccatcg acagtgccag cactgaagat atggatgacg cccttttcgc
    22921 taaggcgctg ccggatgaca aacttcagct gattgtggcg attgccgatc caaccgcgtg
    22981 gattgctgaa ggcagcaagc tggacaaagc cgcgaaaatt cgcgcattca ccaactatct
    23041 gcctggcttc aacatcccta tgctgcctcg cgagctttct gacgatctct gctcactgcg
    23101 cgccaatgaa gtccgcccgg tactggcatg ccgcatgacg ctctccgctg atggcaccat
    23161 tgaagataat atcgaattct ttgccgccac catcgaatcc aaagcgaagc tggtgtatga
    23221 ccaggtttct gactggctgg aaaataccgg tgactggaag cctgaaagtg aagcaattgc
    23281 cgaacaagtc cgtttgctag cgcaaatttg ccaacgccgc ggcgagtggc gtcataacca
    23341 cgcactggtg tttaaagatc gcccggatta ccgctttatt ctcggtgaaa aaggtgaagt
    23401 gctggatatc gtcgccgagc ctcgtcgcat tgccaaccgt atcgtcgaag aagcgatgat
    23461 tgccgctaac atttgtgcgg cccgcgtact gcgcgataag ctcggttttg gcatctataa
    23521 cgtgcatatg ggctttgatc cggcgaatgc cgacgcgctg gcagcgttgc tgaaaacgca
    23581 cggtctgcat gtcgatgccg aagaagtgct cacgctggac ggtttctgca aactgcgtcg
    23641 tgaactggat gcgcaaccaa ctggtttcct cgacagccgc attcgtcgct tccagtcatt
    23701 tgctgaaatt agctctgaac ccggtcctca ctttggcctc ggtctggaag catacgccac
    23761 ctggacttcg ccgatccgta aatatggcga catgatcaac caccgtctgc tgaaagcggt
    23821 tatcaaaggc gaaactgcga cgcgtccgca ggatgaaatc actgtccaaa tggccgaacg
    23881 tcgccgtctc aaccggatgg cagaacgtga tgttggcgac tggttatacg cacgcttcct
    23941 gaaagataaa gccgggaccg gcacccgttt cgcggcggaa attgtcgata tcagccgtgg
    24001 cggcatgcgt gtgcgtttgg ttgataacgg cgctatcgcc tttattccgg cacctttctt
    24061 acacgctgtg cgcgatgaac tggtttgcag ccaggaaaac ggcactgtac aaattaaagg
    24121 tgaaacggtt tacaaagtaa ctgacgttat tgacgtcacc attgccgaag tccgcatgga
    24181 aacccgcagc attattgcgc gcccggtcgc gtaatctcct ttcacggccc attcctcatg
    24241 gatgggccgt ttatttcccc gctctacctt cgttatatcc a
//
LOCUS       DAZPAB010000044        23328 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZPAB010000044 DAZPAB010000000
VERSION     DAZPAB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.044"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..246
                     /locus_tag="ACHUPG_001555"
     CDS             <1..246
                     /locus_tag="ACHUPG_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR0861219.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(293..1051)
                     /gene="deoR"
                     /locus_tag="ACHUPG_001556"
     CDS             complement(293..1051)
                     /gene="deoR"
                     /locus_tag="ACHUPG_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR0861220.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="ACHUPG_001557"
     CDS             complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="ACHUPG_001557"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR0861221.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1990..3216
                     /gene="mdfA"
                     /locus_tag="ACHUPG_001558"
     CDS             1990..3216
                     /gene="mdfA"
                     /locus_tag="ACHUPG_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFR0861222.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3257..3541)
                     /gene="ybjH"
                     /locus_tag="ACHUPG_001559"
     CDS             complement(3257..3541)
                     /gene="ybjH"
                     /locus_tag="ACHUPG_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR0861223.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="ACHUPG_001560"
     CDS             complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="ACHUPG_001560"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR0861224.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4442..5650)
                     /locus_tag="ACHUPG_001561"
     CDS             complement(4442..5650)
                     /locus_tag="ACHUPG_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0861225.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5734..6270
                     /gene="rcdA"
                     /locus_tag="ACHUPG_001562"
     CDS             5734..6270
                     /gene="rcdA"
                     /locus_tag="ACHUPG_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR0861226.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6445..8130)
                     /locus_tag="ACHUPG_001563"
     CDS             complement(6445..8130)
                     /locus_tag="ACHUPG_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR0861227.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8400..8777
                     /locus_tag="ACHUPG_001564"
     CDS             8400..8777
                     /locus_tag="ACHUPG_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR0861228.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="ACHUPG_001565"
     CDS             complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="ACHUPG_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR0861229.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9224..9511
                     /locus_tag="ACHUPG_001566"
     CDS             9224..9511
                     /locus_tag="ACHUPG_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR0861230.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9495..10217
                     /gene="nfsA"
                     /locus_tag="ACHUPG_001567"
     CDS             9495..10217
                     /gene="nfsA"
                     /locus_tag="ACHUPG_001567"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR0861231.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10278..11180
                     /gene="rimK"
                     /locus_tag="ACHUPG_001568"
     CDS             10278..11180
                     /gene="rimK"
                     /locus_tag="ACHUPG_001568"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR0861232.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11268..11744
                     /locus_tag="ACHUPG_001569"
     CDS             11268..11744
                     /locus_tag="ACHUPG_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR0861233.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12096..13208
                     /gene="potF"
                     /locus_tag="ACHUPG_001570"
     CDS             12096..13208
                     /gene="potF"
                     /locus_tag="ACHUPG_001570"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR0861234.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13303..14469
                     /gene="potG"
                     /locus_tag="ACHUPG_001571"
     CDS             13303..14469
                     /gene="potG"
                     /locus_tag="ACHUPG_001571"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR0861235.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLR
                     PINMMFQSYALFPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQ
                     LSGGQRQRVALARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVT
                     HDQEEAMTMAGRIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQE
                     DGLVLDSPGLVHPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHI
                     AYLGDLSVYHVRLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14479..15432
                     /gene="potH"
                     /locus_tag="ACHUPG_001572"
     CDS             14479..15432
                     /gene="potH"
                     /locus_tag="ACHUPG_001572"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR0861236.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15429..16274
                     /gene="potI"
                     /locus_tag="ACHUPG_001573"
     CDS             15429..16274
                     /gene="potI"
                     /locus_tag="ACHUPG_001573"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR0861237.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSLKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16334..16822
                     /locus_tag="ACHUPG_001574"
     CDS             16334..16822
                     /locus_tag="ACHUPG_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR0861238.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16863..17990
                     /gene="rlmC"
                     /locus_tag="ACHUPG_001575"
     CDS             16863..17990
                     /gene="rlmC"
                     /locus_tag="ACHUPG_001575"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR0861239.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18165..18896)
                     /gene="artJ"
                     /locus_tag="ACHUPG_001576"
     CDS             complement(18165..18896)
                     /gene="artJ"
                     /locus_tag="ACHUPG_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR0861240.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19188..19856)
                     /gene="artM"
                     /locus_tag="ACHUPG_001577"
     CDS             complement(19188..19856)
                     /gene="artM"
                     /locus_tag="ACHUPG_001577"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR0861241.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19856..20572)
                     /gene="artQ"
                     /locus_tag="ACHUPG_001578"
     CDS             complement(19856..20572)
                     /gene="artQ"
                     /locus_tag="ACHUPG_001578"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR0861242.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20579..21310)
                     /gene="artI"
                     /locus_tag="ACHUPG_001579"
     CDS             complement(20579..21310)
                     /gene="artI"
                     /locus_tag="ACHUPG_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR0861243.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21328..22056)
                     /gene="artP"
                     /locus_tag="ACHUPG_001580"
     CDS             complement(21328..22056)
                     /gene="artP"
                     /locus_tag="ACHUPG_001580"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR0861244.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22274..22789)
                     /locus_tag="ACHUPG_001581"
     CDS             complement(22274..22789)
                     /locus_tag="ACHUPG_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0861245.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT         5733 a         5753 c         6027 g         5815 t
ORIGIN      
        1 gtgaccatac cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg
       61 cagcttaccg caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac
      121 ggtaaatcca ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc
      181 tttggtagga tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc
      241 tcttaatctt ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca
      301 tcaacttaat acggtgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcac
      361 tcaccacaat atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt
      421 tactgtggtc gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg
      481 gcaactcttc aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcggaat
      541 aaaaagcgat atccgggcaa aaattattca acgtttgctg aaaatcgatg ggtttgaaaa
      601 tggcgttgct ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttctctt
      661 tcagcgccag aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa
      721 tcgcttcaat aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt
      781 ctaccagtgt cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt
      841 gatcgcttaa caggtaatgg ctggcactgc gcggttccag aacaatatag ccgccgagca
      901 aaacgacggg cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa
      961 gcagggcggc ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct
     1021 gcccgatacg ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc
     1081 cgctcaatac tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta
     1141 atgcaaaaca gacgcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt
     1201 gccagataat ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat
     1261 ccagcggcca gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca
     1321 ttaaaagtga gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa
     1381 tcaccgtacc gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc
     1441 cgatattttc gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa
     1501 acaggctgac cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc
     1561 cccacaacca aagtaccgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa
     1621 aaatcgccaa cgagatcatc cacggagccg agtctggcgt cgcgttaata agagagaata
     1681 gagagagatt caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa
     1741 tacaggcaag tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat
     1801 ttccctcaag cggcctgttt acggtgggtg attgtaacgg gcataggtta aataaaactt
     1861 aaagaaagcg tagctatact cgcaataatg taagaatgtg cttaaccgtg gtttcagcta
     1921 caaaattcgc tttctcgtta gctgcgcttt tattaaactc tgcgcgatta ttattggcga
     1981 agaaattgca tgcaaaataa attagcttcc ggtgccaggc ttggacgtca ggcgttactt
     2041 ttccctctct gtctggtgct ttacgaattt tcaacctata tcggcaacga tatgatccaa
     2101 cccggtatgt tggccgtggt ggaacaatat caggcgggca ttgattgggt tcctacttcg
     2161 atgaccgcgt atctggcggg cgggatgttt ttacaatggc tcctggggcc gctgtcggat
     2221 cgtattggtc gccgtccggt gatgctggcg ggtgtggtgt ggtttatcgt cacctgtctg
     2281 gcaatattac tggcgcaaaa cattgaacaa ttcaccctgt tgcgcttctt gcagggcata
     2341 agcttctgtt tcattggcgc tgtgggatac gccgcaattc aggaatcctt cgaagaggcg
     2401 gtttgtatca agatcaccgc gctgatggcg aacgtggcgc tgattgctcc gctacttggt
     2461 ccgctggtgg gcgcggcgtg gatccatgtg ctgccttggg aggggatgtt tgttttgttt
     2521 gccgcattgg cagcgatctc ctttttcggt ctgcaacgag ctatgcctga aaccgccatg
     2581 cgtataggcg agaaactgtc actgaaagaa ctcggtcgtg actataagct ggtgctgaag
     2641 aacggccgct ttgtggcggg ggcgctggga ttcgttagcc tgccattgct ggcgtggatc
     2701 gcccagtcgc cgattatcat cattaccggg gagcagttga gcagctatga atatggcttg
     2761 ctgcaagtgc ctattttcgg ggcgttaatt gcgggtaact tgctgttagc gcgtctgacc
     2821 tcgcgccgca ccgtacgttc gctgattatt atgggcggct ggccgattat gattggtctg
     2881 ttggtcgctg ctgcggcaac ggttatctca tcgcacgcgt atttatggat gaccgccggg
     2941 ttaagtattt atgctttcgg tattggtctg gcgaatgcgg gactggtgcg attaaccctg
     3001 tttgccagcg atatgagtaa aggtacggtt tctgcggcga tgggaatgct gcaaatgctg
     3061 atctttaccg tcggtattga aatcagcaaa catgcctggc tgaacggggg caacggactg
     3121 tttaatctct tcaaccttgt caacggaatt ttgtggctgt cgctgatggt tatcttttta
     3181 aaagataaac agatgggaaa ttctcacgaa gggtaaaaaa atgcctgatt gatttgtgcg
     3241 atcaggcatt ctcgaattaa tggtgatggt cgtcaatctg gtgttcgata accatccctt
     3301 cacctacgct ggcaagatgg cgaacataag gatgcgggcg gtaagccgga gctggcgcag
     3361 gagccacata gacggtttgc ggcacagtcg cgacgctgac cgcttgtgga acgctgactg
     3421 aatcaggaac cacaaccact ttgtatccac tcggcacatc gacggtgaca ctttgcgcca
     3481 tcgccacacc agtaaagccc attaaaagcg cgcccaacag tagacaattt ttcataatca
     3541 tttcgcctga aataatgcag cgggttaatt tccgcgaatt atgcagcctt cattcaggca
     3601 ttgtgttgcc gggcgtgctg ccgtttttat tggtcaaatg gcgcttcgtg tttaagaact
     3661 ttatcgatca catccagtac gccttcacgg ttattgtagc ctgcccggta ttttgccgct
     3721 gcgacgactg cgctgccggc attttccatt gcaaaactaa agcctgcctg acgcagcatc
     3781 tcaatatcgt taccgccatc gccaaagacc accacttcgc tgtcgtctat tccccataat
     3841 ttctgcagtt ggcgaaggcc attggcttta tgtacgccgg ggataatcag atcgatgctg
     3901 ccgttgccgg tgtggactgg caccataata tcgccgatgg cctcatgtaa tgctttttgt
     3961 acttgtggaa tcagttcatc ggaaagattc aggccaaact taaagaaaat atcttctaag
     4021 ttgtcaaagt tatcgacgta ttccagacgg tgataataca tttccgccac cgttttcatg
     4081 gcatcgtcat attttttgag tgtataggcg ctattttttc cgcaggcaat aatttccact
     4141 tccgggcgcg tcagcaaatg ttccacgaca gtagcaaacg catccttcga cagctcgcca
     4201 ttaaaaacat ctttgccttc actcattacc cagccgccgt tttcagccac aaaggcaatt
     4261 tcattcgcaa tttcagggaa gaaagagatc aactgataat attgattccc gctggcgacc
     4321 acaaagcgaa tgccttgtgc tttcatttgc tgatactgag ccataaaccg ctcacggtta
     4381 taggtttttt gatcgcttaa gaaagtacca tccatgtcta ccgcaattaa tttaatgctc
     4441 atcaactatt ctccatcgcc gtctgcgttt tggtatcggg tttggcgacg gctttcgcga
     4501 caatagccgc gagaataacc agcgccagta caaccagcat tgcactacgt aatccataat
     4561 gttcgccgag atagcccagc agcggcggcc cgacgaggaa agccagataa ccggtcgttg
     4621 ctaccacact gacgcgggtt ggtgcatcgg ggccggtatc gctggcggca gaaatggtca
     4681 gcgggaagcc cagcgaggca cccagtcccc aaagtacaac agacactcct gcgacccagg
     4741 cgctatcgac aaaaataatc agcccaatac ccaacgcccc cattagtgca ctggcccgaa
     4801 ccacggcaac gcgactgtaa ctgtcgatga accaaccgcc ggtgaagcgt ccaacggtca
     4861 tccctagggt aaaaccggca taaatcagtg agccggaagt ggggctaaaa ccgtgaccat
     4921 caaccattaa taagggtaac cagtcgttgg cagaaccttc ggcaaaggcc atcgccagca
     4981 ctacaacacc aatcaacagc aactggatat cgcgataaaa aggaatgcct ttttcgccat
     5041 gctgggtgcc atcggcagca tttttgcccg taccgtcagg gattgcctga atggcgatat
     5101 aaataggcgc gatacctacc agcgccgcca ataaaatgtg caccgttgcc ggaacgccaa
     5161 aggccgtcag tgccatcccg acaccagcgc ctgccagcgt gcccaggcta taaaaaccgt
     5221 gcatcatcgg caaaaccgtt ttattcattt ctcgctcaac ggcggcacct tcaacgttta
     5281 tcgccacttc cgcagaacca aaacttgccc caaagacgcc gagaccaacg gcaaagagca
     5341 ggggcgatgt cagccagagt gccagactta atatcatcat cccgatcaat gcgcaggaca
     5401 tcgtgaccag gatgacatta cgtgtcccaa agcgtttcac taaccacgcc gagcagagaa
     5461 taccgctcat cgaaccgatc gacagaccaa agagaacacc gcccatttca gcgatcgaga
     5521 cagagagaat atcgcggata gcaggcgtac gggttgccca ggacgccatt aacaggcctg
     5581 gcaaaaagaa gaacataaac agcgcccagg ttcggcgttt caatgcatta cgtgaagaat
     5641 ttacggtcat agatcacgtc aaaataagaa gagggaagac aacattagca aggttgtgta
     5701 catttgtaca caattgcaga cagaggaaat gacatgcgtc gcgctaacga tccgcaacgg
     5761 cgagaaaaaa ttatccaggc cacgctggag gcggtgaaac tttacggaat acatgctgtt
     5821 acgcaccgca aaattgctac ccttgccggg gtaccgttgg gatcgatgac ctactatttt
     5881 tcaggaattg atgagttgtt gctggaggcg ttcagcagtt ttactgagat tatgtcccgg
     5941 caatatcagg cattttttag cgatgttagt gatgctcaag gcgcatgcca ggctatcacc
     6001 gatatgatct acagctcaca ggttgcaacg ccggataata tggagctgat gtaccagctc
     6061 tacgcgctgg ctagccgaaa accgctatta aaaacggtaa tgcaaaactg gatgcagcgc
     6121 agtcagcaaa cgctcgaaca atggtttgaa cccggaaccg cccgcgcgct tgatgcgttt
     6181 attgagggga tgacgctgca ttttgtcacc gaccgtaagc cgctatcgcg cgaggagatt
     6241 ttgaggatgg ttgagagggt tgcagggtag tagataaatt ttagataaca aaaaacccat
     6301 caaccttgaa ccgaaatggc ggggttgatg ggctccacaa aatggggaca tcaaagaaaa
     6361 gcagtggcac taattaagac tgatgccctg cggaaaagtt ctgcggttgt gcaaaaaaat
     6421 ttcattttca gggcaacttc agttttatcc taatcctggc cataccatga cgatgattgt
     6481 ccctgccagc gtcagcagga cgttggcgat tgcgtaggtg cccgcatagc ccagcgcagg
     6541 gatgttactg cgagctgtat cactgatgat ctccattgcc ggcgcgcagg tgcgtgcgcc
     6601 catcattgcg ccgaacaaca gtgcgcggtt cattcgcaat acataagcac cgaacaagaa
     6661 acagataacc acgggcacaa gactgacaat taatccggca atcaacatct gaccgccaat
     6721 cgcgcccagg ccgttattaa taccgctacc ggcgctcaga ccaacgcctg ccataaacac
     6781 catcaagccg aactctttca ccatgcttaa tgcaccttgc ggaatgtaac cgaaggtcgg
     6841 gtggttagca cgcataaagc ccagcataat tccggcgaat aacaacccgg cagcgttccc
     6901 catgccgaaa ctgaatgtgc tgaactggaa ggtgatcatc ccgatcatca gcccaataac
     6961 aaagaaggcg caaaatgcca gcaggtcagt gacctggctg tgaatcgaga taaagccgat
     7021 gcgatcggcg atggttttta cgcgacgggc atcaccgctg acttgtaaaa cgtcaccttt
     7081 gttaagcacg acgttgtcat ctatcggcat ctcaatctgg ctacgaatga cgcggttaag
     7141 gaagcaaccg tgatcggtca acttcagttg tgcgagacgt ttacctacag cgttatggtt
     7201 tttaacgacc acttcttcag tgacgatacg catgtcgaga aggtcacgat cgaaaacttc
     7261 tttaccgtta cggaagctgg gatcgagtcg ggcatgggcg tcgggatagc ctaccaacgc
     7321 tatttcatcg cccatttgta gcacggcatc accgtctgga tttgccagaa tcccgttacg
     7381 tcgaatacgt tcaatgtagc agccggtttg tcgataaata cccagttcac gcagattttt
     7441 gccgtcggtc caggccacca gctccgggcc gacgcgatag gcgcggatca ccggtaaata
     7501 aaccttacgg ttggcatcag tgtccaggcc acgttcgcgg gcgatttgct gggcgctggt
     7561 ctgtaagtcc tgatgctgca atttcggcaa gtaacgcgca ccaacaatca aactcaccag
     7621 accgattaaa taggttaagg catacccgag gctcagatta tccagtgcca gtgagagctg
     7681 cctgctttcc atgccggaat gatgcagtgt atcgccagca ccgaccagaa ccggtgtcga
     7741 cgtcatagag cctgctaaca taccggccgt caggccaata tcccagccaa acagcttacc
     7801 taaccctaag gcgatcacca gcgcactgcc aaccatcacc agtgctaaca ttaggtaatt
     7861 tttcccatcg cgaaaaaaaa tggaaaaaaa gttcggtccg gcttcgaccc cgacgcagaa
     7921 aataaacagc ataaagccaa gattaagcgc atcggtgtta atgctgaaat gttgttggcc
     7981 taataacagc gatacgacta aaacgccaat ggaattaccc agttggatcg aaccaagtcg
     8041 taactttccg agacatagcc caagcgcgag gaccacaaat aataacagaa tgtaattccc
     8101 atttaacaat tcggcgacgt ttatattcac ggaggctaac ttcttgttta ctagtaagct
     8161 gttgaaagaa atgctaattt acgataatgt gttttaccag aattcagggc gcagattcat
     8221 tcagcgcacc taaacgatag taaagtaaca atatatttta ctagtgtaat cacattaggt
     8281 atcaacggct atatgaattg cgttggccta tattagcatg gaatgcgaag cggctttatc
     8341 ttactgaacg ccacactggc gaaaaatgtg ttcgatagac gcagtgtcag gaggaacgag
     8401 tgaaacataa acaacgttgg gcgggggcaa tctgctgttt tgtcctcttc attgtggtgt
     8461 gcctttttct ggcgacgcac atgaaaggcg cttttcgggc tgccgggcat cctgaaatcg
     8521 gcttgctgtt tttcattctt cctggagcag ttgccagttt cttttcacag cgtagagaag
     8581 tcctgaaacc tctatttggc gcaatgctgg cggcaccctg ttcaatgctc attatgcggc
     8641 tgtttttttc accgacgcgc tcattctggc aagagctggc atggttacta agcgcggtgt
     8701 tctggtgtgc gctgggggca ctgtgtttct tatttatcag tagtttgttt aaaccacagc
     8761 acagaaaaaa tcagtaaagc cctcaacgcg agggcttgtc agacgatcag gcgtccagat
     8821 tttctttcac ccatgcagca aaatcggtat agccgccgat atgttgctga tcgacaaaaa
     8881 tctgcggcac ggtttctacg ggtttacctg ccttttgttg tagatcttct ttagtgatcc
     8941 cttccgcacg aatatctaca tactgatact gaaaatcatc gcgttcattg ctcaatttct
     9001 cagccagatc ttttgcacgc acacagtaag ggcaacccga acgaccaaaa ataacggttt
     9061 gcattatttc tctcctcata gatttatgcc tgtaatgatc acgctaaaat gtattcgctg
     9121 aaagtaggtt taacctgttg cattaattgc taaaagctat aactgttaaa cacaatacag
     9181 tgaaaagttt tagactgaag gctcactttg cagagggaag cgtatgcgcg cgatcggtaa
     9241 attgcctaaa ggcgtgttga tactggaatt tatcggaatg atgctactgg cggtggcgct
     9301 gctgtcggta agcgactccc tgtcgctgcc tgagccattt tctcggccag aagtgcagat
     9361 tctgatgatt tttctcggtg ttttgctcat gcttcccgct gcggtggtgg ttattcttca
     9421 ggtggcaaaa cgtcttgccc cacagctgat gaactgtcca ccgcaatatt cacgttcaga
     9481 aagagaaaaa gataatgacg ccaaccattg aacttatttg tggccatcgc gccattcgcc
     9541 atttcactga tgaacccatt tccgaagcgc agtgtgaggc gattattaac agcgcccgtg
     9601 cgacgtccag ttccagtttt ttgcagtgta gtagcattat tcgcattacc gacaaagcat
     9661 tacgtgaaga actggtgacg ctgaccggcg ggcaaaaaca cgtagcgcaa gcggcggagt
     9721 tctgggtgtt ctgtgccgac tttaaccgcc atttacagat ctgtccggat gctcagctcg
     9781 gcctggcgga acaactgttg ctcggtgtcg ttgatacggc aatgatggcg cagaatgcat
     9841 taaccgcagc ggaatcgctg ggattgggcg gggtatatat cggcggcctg cgcaataata
     9901 ttgaagcggt gacgaaactg ctgaaattac cgcagcatgt tctgccgctg tttgggctgt
     9961 gccttggctg gcctgcggat aatccggatc tcaagccgcg tttaccggcc tccattttgg
    10021 tgcatgaaaa cagctatcaa ccgctggata aagacgcact ggcgcagtat gacgagcaac
    10081 tggcggaata ttacctcacc cgtggcagca ataatcgctg ggatacctgg agcgatcata
    10141 tccgccgaac aatcattaaa gaaagccgcc catttattct ggattatttg cacaaacagg
    10201 gttgggcgac gcgctaaaac cgccacgtcg atgtatgata cgcgggcttt tgaccaggtc
    10261 tgacagagag gtgcagggtg aaaattgcca tattgtcccg ggatggaacg ctctattcgt
    10321 gtaagcggct gcgtgaagcc gctatacagc gcggtcacct ggttgaaatt cttgatccgc
    10381 tttcttgcta catgaacata aatcctgcgg cgtcttctat ttactacaaa ggccgcaagt
    10441 taccccattt tgacgcagtg atcccgcgta ttggcaccgc cattaccttt tatgggacgg
    10501 cggcactgcg ccagttcgag atgctgggga gctatccgct caatgagtcg gtcgccattg
    10561 cccgggcgcg tgacaaattg cgttccatgc aactgctggc gcgtcagggc atcgacctgc
    10621 ctgtcacggg cattgcgcat tcgccggatg ataccagcga tttaatcgac atggtcggtg
    10681 gtgcgccgct ggtggtcaag ttggttgaag gcacgcaggg aattggcgtc gtgctggcgg
    10741 agactcgtca ggcggcggaa agcgtgattg acgctttccg cggtctgaac gcgcatattc
    10801 tggtgcagga atatatcaaa gaggcgcaag ggtgcgatat ccgctgtctg gttgttggtg
    10861 atgaagtggt cgctgcgatt gaacggcggg cgaaagaggg ctattttcgt tccaatttgc
    10921 atcgtggcgg cgcggcaagc gtcgccagta tcacaccaca ggagcgtgaa atcgcgataa
    10981 aagccgcgcg aacgatggcg ctggacgttg ctggtgtgga tattctgcgt gctaatcgcg
    11041 ggccgttggt gatggaagtg aatgcgtcgc cggggctgga aggaatagaa aaaaccaccg
    11101 gtatcgatat cgcgggtaaa atgatccgct ggatcgaacg ccacgctacg acagaatatt
    11161 gcctgaaaac gggtggttag tcgcaatcac attactgatc atggttttgc ctgcgctttt
    11221 tgcgtaagct gtgccggtct ttttatcgaa agaggttgta caaaattatg acatcgctgg
    11281 tcgttcctgg tctggatacg ctgcgtcaat ggctcgatga cctggggatg agtttttttg
    11341 aatgtgataa ctgtcaggct ctgcatctgc cccatatgca gaatttcgac ggtgtctttg
    11401 atgccaaaat cgatctgatc gataacacga tcctgttttc tgccatggcg gaagtccgac
    11461 cttcagccgt attgccgctg gcggcggatt tatctgccat caatgccagt tcgctgaccg
    11521 tgaaagcatt tcttgatatg caggatgata atctgccaaa gctggtggtt tgccagtctt
    11581 tatccgttat gcagggcgta acctatgagc agtttgcatg gttcgtgcgt cagagcgaag
    11641 agcagatttc gatggtcatt cttgaagcta atgcccatca actgctgtta ccgactgacg
    11701 atgaagggca aaacaacgtt accgaaaact attttctcca ctgataattc ctttcgagca
    11761 cgcagtcgct gccgcagtga ctgcgcgctg caaaattatc tgctgttttt aaccttttct
    11821 taaagattat ttcacttctc ttgtgtcgat ttggctttat cacatagagc aaatacgcat
    11881 aaaaatttgt taaataccgt tttttaatcc gagctatagt ctcaaaccct ggctaaagtt
    11941 attcttgcga tgcttctata tagcgagcag tgctggccgg gagagagttc tcttttctta
    12001 caccgcgccg ataaaaaaat atgcacgttt attgcatatc tttcagtgtg acaacttttg
    12061 ttcgtttgtt aacgaacttt cagaaggaaa gagatgtgac cgccttaaat aaaaaatggc
    12121 tatcgggtct ggttgcgggt gctctgatgg ccgtctctgt cggcacgctc gcggctgaac
    12181 aaaaaacact ccacatttat aactggtctg attatatcgc ccctgacacg gtggccaatt
    12241 ttgaaaaaga aaccggtatt aaagtcgtct acgatgtttt cgactctaac gaagtactgg
    12301 aaggcaaatt aatggcgggt agcaccggct ttgacttggt ggttccatct gccagctttc
    12361 tagaacgcca gttgactgcg ggagttttcc agccgctgga caaaagcaaa ttgccggagt
    12421 ggaagaatct cgacccggag ctgctgaagc tggtcgccaa acacgatccc gacaataaat
    12481 ttgctatgcc ctatatgtgg gcgacgaccg ggattggcta taacgttgat aaagttaaag
    12541 ctgtgctggg cgaaaacgcg cccgtcgata gctgggactt gatcctcaaa cctgaaaatc
    12601 tggaaaaact gaaaagctgc ggtgtctctt tcctggatgc gccagaagaa gtttttgcta
    12661 ccgtgttgaa ttatctcggc aaagatccca acagcactaa agcggatgat tacaccggac
    12721 cggcaaccga tctgctgtta aagctgcgcc cgaacattcg ttatttccat tcatctcaat
    12781 acattaacga cctggcaaac ggcgatattt gcgtcgctat cggctgggca ggtgatgtct
    12841 ggcaggcgtc aaaccgcgcg aaggaagcga agaatggagt gaatgtctcg ttctcgattc
    12901 caaaagaagg ggcgatggcg ttctttgatg tattcgccat gccagcggat gccaaaaaca
    12961 aagacgaagc ctatcagttc ctgaattacc tgctgcgccc ggatgtagta gcgcatattt
    13021 ccgaccatgt gttctatgtc aacgccaata aagcagccac gccgctggtg agtgcggaag
    13081 tccgtgagaa cccgggtatt tatccgcctg cggatgttcg tgcgaagctg ttcactctga
    13141 aagtgcagga cccgaaaatc gaccgtgtgc gcacccgcgc gtggaccaaa gtgaagagcg
    13201 gaaaataatc cgcagcctta gatgccgggc gggcgcacca cacccgccgg caattcgcac
    13261 catcatggtg cgcttgcaca cattcaatgc cggagagcag ccgtgaatga cgctatccct
    13321 cgcccgcagg cgaaaacccg taaggcgctg acgccgctat tagaaatccg caacctcacc
    13381 aaatcctacg atggtcaaca tgcggtggat gatgtcagcc tgaccatcta caaaggtgaa
    13441 atcttcgcgc tgctgggcgc atccggctgt ggcaagtcca cgctgctggg cgcatccggc
    13501 tgtggcaagt ccacgctgct gcgtatgctg gcaggtttcg aacaaccttc tgccggacag
    13561 ataatgcttg atggcgtcga tttgtcacag gttccgcctt acctgcgccc catcaatatg
    13621 atgtttcagt cttacgcgct gtttccgcat atgaccgtgg aacagaacat cgcttttggc
    13681 ctgaaacagg acaaactacc gaaagcggaa atttccagtc gggtcaatga gatgctcggc
    13741 ctggtgcaca tgcaggagtt cgccaaacgt aaaccgcatc agctttctgg tggtcagcga
    13801 cagcgtgtgg ccctggcccg aagccttgcc aagcgcccga aactattact gctcgacgag
    13861 ccgatgggcg cgctggataa aaagctgcgt gaccggatgc agcttgaagt ggtggatatt
    13921 ctggagcgcg tcggtgtgac ttgtgtgatg gtcacccacg atcaggaaga ggcgatgacc
    13981 atggcggggc gcatcgccat tatgaatcgt gggaaatttg tccagattgg cgaaccggaa
    14041 gagatctacg agcatccgac tacccgctat agcgccgaat ttattggctc ggtaaacgtc
    14101 tttgaaggcg tactcaaaga gcgtcaggaa gatggcttgg tgcttgattc gccggggctg
    14161 gtgcatccac tgaaagtcga cgcggatgcc tcggtggtcg ataacgtgcc ggtacatgtg
    14221 gcgctgcgcc cggaaaaaat catgctttgc gaagagccgc ccgccaatgg ttgtaacttc
    14281 gcggtggggg aggtgataca cattgcctat ctcggcgatc tttcggtgta tcacgttcgt
    14341 ctgaaaagtg ggcagatgat tagtgcccag ctacaaaacg cccatcgtca tcgtaaaggg
    14401 ttaccgacct ggggcgacga agtgcgtttg tgctgggaag tggacagctg tgtggtgctg
    14461 acggtttaag gagcaaagat gaatacactt gaacctgctg cccagtcgaa accgccgggc
    14521 ggatttaagc tgtggttgtc gcagctgcaa atgaagcatg ggcgcaaact ggtcattgcg
    14581 ttgccatata tctggttgat cttgctgttt ctgctgccat ttctgattgt ctttaaaata
    14641 agcctggcgg agatggcgcg cgctattcca ccttataccg aactgatgga gtgggctgac
    14701 gggcaactgt ccatcactct taatctcggt aattttctgc aactgaccga cgatccgctc
    14761 tatttcgatg cttatctcca gtcgctacag gtggctgcga tttcgacatt ttgctgttta
    14821 ctgatcggct atccgctggc gtgggcggtg gcgcacagta agccttcgac ccgtaatatt
    14881 ttattactac tggtgatcct gccgtcgtgg acctcgtttc tgatccgcgt ttatgcttgg
    14941 atgggaatat taaaaaacaa cggtgtgctg aataattttc tgctgtggct gggggttatc
    15001 gatcaaccgc tgaccattct tcataccaat ctggcagttt atattggcat tgtttacgcc
    15061 tacgtgccgt ttatggtgct gccgatttat accgcgttga ttcgtattga ttattcgctg
    15121 gtggaagcag cgctggatct cggtgcacga ccgctgaaaa cgttctttac tgtgatcgtg
    15181 ccgctgacta aaggtgggat tattgccgga tcgatgctgg tgtttatccc ggctgtgggc
    15241 gagtttgtga tcccggaact gctcggtggc ccggacagca tcatgatcgg gcgcgtgcta
    15301 tggcaagagt ttttcaataa ccgcgactgg ccggtggcct cggcggtagc gatcatcatg
    15361 ttgctgctgc taattgtgcc gataatgtgg ttccacaaac accagcaaaa aagcgtggga
    15421 gaacacggat gaataattta ccggtagttc gttcgccctg gcggattgtg attttgctgc
    15481 tgggctttac ctttctctac gcgccaatgc tgatgctggt tatctattcg tttaacagct
    15541 tgaagctggt gacggtgtgg ggcggctggt caacgcgctg gtatggtgag ttattgcgcg
    15601 atgacgcgat gatgagtgcg gttggttcaa gcctgacaat tgctgcctgt gcggcaacgg
    15661 cggcggcgat cctcgggact attgcggcgg tggtgctggt gcgctttggc aggtttcgcg
    15721 gatcaaatgg ctttgccttt atgatcaccg cgccgctagt aatgccagat gtcatcacgg
    15781 gcttgtcgct gttgttatta ttcgtcgcgc ttgctcatgc cattggctgg cctgcggacc
    15841 gcggtatgct taccatctgg ctggcgcatg tcactttctg tacggcttat gtggcggtcg
    15901 ttatttcgtc gcgtctgcgg gaactggatc gctcgataga agaagccgcg atggatctcg
    15961 gtgcgacgcc gctgaaagtg tttttcgtca ttacgctacc gatgatcatg cccgcgatca
    16021 tttctggctg gttactggct tttactttgt cgcttgatga tctggtgatc gccagctttg
    16081 tttccgggcc gggagccacc acgttaccga tgctggtctt ttccagcgtg cggatggggg
    16141 tgaatccgga aatcaacgcc ctggcaacat tgatcctcgg cgcggtcgga attgtcggat
    16201 ttatcgcctg gtatctgatg gcgcgcgcag aaaaacagcg gatacgcgac atccaacgtg
    16261 caagacgtgg ctgaagacac taaaatttgc caacctggct acataatgcc gcgcatgtcg
    16321 cggcattgtt ttcatggaag acgaaacgtt gggatttttt aagaaaacat cttcatctca
    16381 tgctcgcctg aatgtgcctg cgctggtgca ggtggcggcg ctcgccatta ttatgatccg
    16441 tggcctcgac gtgctgatga ttttcaatac gctgggcgtg cgcggtattg gcgagttcat
    16501 tcatcgcagc gtacaaacct ggagtttaac gctggtcttt ttaagcagtc tggtgctggt
    16561 tttcattgag atctggtgtg cgttttcact ggtgaaaggg cgtcgctggg cgcgctggct
    16621 atatctgctg acacaaatca ccgccgcaag ttacttgtgg gcggcttcgc tggggtacgg
    16681 ttatccggag ctgttcagca ttcccggtga atcaaaacgt gaaatcttcc atagcctgat
    16741 gctgcagaag ctgccggata tgctcatcct gatgctgctg ttcgttccct cgaccagtcg
    16801 gcggttcttc cagttgcaat aatgtgtata atcgtcgccc ctgatgatgt gaaggtcaat
    16861 gtatgcagtg cgcactttac gacgcgggtc gctgtcgttc ctgtcagtgg ataacgcagc
    16921 cgattccaga gcaactctcc gctaaaaccg ccgatcttaa aaatctgctc gccgattttc
    16981 cggttgagga atggtgcgcg ccggtgtcag gcccggaaca agggtttcgt aataaagcca
    17041 aaatggtggt gagtggtagc gttgaaaaaa cactgctcgg tatgctgcat cgcgatggca
    17101 cgccggaaga cctttgtgac tgcccgcttt atccagcctc atttgcgccc gtttttgcgg
    17161 cgctaaaacc gtttatcgcc cgagcggggt taacgcccta caacgtggcg cgtaagcgtg
    17221 gcgaactgaa atacattctg ctgactgaaa gccagagtga tggcggcatg atgctgcgct
    17281 ttgtactgcg ttctgatacc aagctggcgc aactgcgtaa ggcgctgccg tggttacacg
    17341 aacaactacc gcagctgaaa gttattaccg tcaatattca gccggtacat atggcgatta
    17401 tggaagggga gacggagatc tacctgaccg aacaacaggc actggcggag cgttttaatg
    17461 acgtaccgct gtggatccgt ccgcaaagtt tcttccagac taatccggcg gtcgccagcc
    17521 agttgtacgc caccgcgcgc gactgggtac gacagctgcc ggttaaacat atgtgggatc
    17581 tgttctgcgg tgtggggggc tttggtttac actgcgcgac gcctgacatg cagttaaccg
    17641 ggatcgaaat tgcgccagag gccattgcct gtgcaaagca gtcagccgct gaactgggct
    17701 taactcgttt acaatttcag gcgctggact ccactcagtt tgccactgct cagggggagg
    17761 tgccggagct ggtgctggtt aacccgccgc gccgcggcat tggtaaaccg ctgtgtgatt
    17821 atctttcaac gatggcaccg cgttttatta tctactccag ctgtaacgcc caaaccatgg
    17881 cgaaagatat ccgcgaactg ccaggttacc gcatcgaacg ggtacagctt tttgatatgt
    17941 tcccgcacac cgcgcactat gaagtgttga cgctgctggt gaagcaataa aaaagccaca
    18001 ggtgcggctt cagattgctg atcaggtgcg ctttgttcat gccggatgcg gcgtgaacgc
    18061 cttatccggc ctacaaaatc atgctaaatc aatacattgc agagttgatg tgggccggat
    18121 aagcgcagcg catcaggcaa tcttactttt gtcatcagtc tcaattactg cggaaaccac
    18181 tggtcattga ttttctgata tgtaccgtca gctttaattg ctgccagcgc gttattcagt
    18241 ttttccagca gagctttgtt atccggacgt acagcgatac ccaggccggt gccgaagtat
    18301 tgcggatcgg tcactttttc agtcgcaacg cccagttgcg gattggtttt cagccattcg
    18361 tttaccaccg cggtgtcacc aaatacccca tcaatacgac catttttcag atcgataaag
    18421 gcattctgat aactgtcata ggagacagtt ttcacttccg ggtgctgatc ctgaatatat
    18481 ttctggtgcg tcgtaccgtt ttccatccca atgcgtttgc ctttcaggtc ggcaaacgtt
    18541 ttgtaggtat cttttttgga aatcacgacg gctgagtttt catagtaggg tgtggtaaac
    18601 gaaacctgct tgctacgctc cggggtgata tccataccgg agattacggc gtcatatttt
    18661 ctgaatttca gtgacgggat caggctgtcg aacgcgtgat tagtaaaagt acattctgcc
    18721 tgcatttgtt tgcacaaggc ttttgccaga tcgatatcaa agccgacaat ctcattatta
    18781 gcacctatag attcaaaggg tggataggtg gctgaaacgc caaaattgat tttctctgcg
    18841 gcagaagcac cgaaagtaaa ggaagcaagt aaagcggcaa gaactaactt tttcatgatg
    18901 gaactcccgt ctgtcaatct tatgattttt ggccgtgtct gcggcatggg ataacaatgc
    18961 catcaagtga atttatatgc aataaacatg attaaataat ttaaatgaaa taaaaaaaga
    19021 cggataactt agtgagttgt ccgtcttcat tataagaatt tatgcactat gtaggccgga
    19081 taaggcgtcc acgccgcatc cggcacaggc accgtgctga tgtctgatgc gacgctggcg
    19141 cgtcttatca gacctacaaa cccccccggc gaatgtacgc agctacatta attccgccgt
    19201 tcgaatgcca gtgctttgcg ctcgatcaga cgcatcatca gcgtcagcag gccgttgacg
    19261 accaagtaaa taatccctgc cgcaccgaac accattacat cgtaggtgcg tccgtacaac
    19321 aactggctgt atcccatcac ttccatcagc gtaatggtgt aagccagaga ggtacttttg
    19381 aacaccagca ccacttcgtt ggaataagaa gagagcgagc gtttaaaggc atacggcagc
    19441 aggatcgcca gcgtatcttt tttgctcatt cccagggcgc tacaggactg ccactgacct
    19501 tctgggatcg cacgaattgc accgtaaaac agctgcgtgg tatacgccgc actgttcagc
    19561 gataacgcaa tcagcgcaca taaccacggt tctgacaaca aatgccacag tgccggatac
    19621 tcctgcaacg tcggaaactg gcccggcccg taataaatca ggaagatctg caccagcagc
    19681 ggcgtaccgg taaacagcgt gatataaccc cgcaccagcc acaccagcac cggcgttttc
    19741 agcgtcagga tgatggtaaa aatcaatgcc agaatcagtg ccacaatcag cgaggcaacg
    19801 gttagcgtca ggctggtgtg cagccctttc atcagctcgg gtaaatactc aaacattagc
    19861 tgggcctccg ctcaaaacgt gtcgcgcgca ggtcaatgcg tttgagaatg tactgactga
    19921 gcagggtgat caccaggtaa atcgccgccg ccacaatgta ccaggtaaat ggttcctggg
    19981 tacgagtagc aatgcttttg gtttgtaaca ttaaatcatt cacactaatc aaactgacca
    20041 gcgcggtatc tttcagcagc accagccact ggttaccgag gccaggcagc gcatgacgcc
    20101 acatctgcgg catcaccaga cggaaaaaga tagccgattt cgacagcccc agcgcctgac
    20161 cagattccca ctggcccacc ggtacagctt tcaacgcgcc gcgcagcgtt tgcgaggcat
    20221 aggcggcata cagcagtgac agagcgatga caccacaaag gaacggactc acgtcgaagt
    20281 tctcaatgtc catctgcacc gggatctgca cgaacccaag attgatagtg aagccatccg
    20341 aaagcgtcag cagtaactgc gaggagccaa aatagataaa cagcactacc agaatttctg
    20401 gcaggccacg cagaatggtt accagcgctg aacctgccca cgcgacagga cgccatttcg
    20461 ctgactccca taccgcaaag aacatcgcca gcgccagccc gacaatcaat gcacaaacgg
    20521 caaggccgac ggtcatcccg gcggcgcttg ctaaaggaaa aaattcattc atcaggaatt
    20581 acttctggaa ccatttgttg tagatggttt cgtaagtgcc atctttcttc actttttcca
    20641 gcgcagtgtt gagtttctgc tgcagttcag tgtttccctg acgtaccgcg atgccgaggc
    20701 cagtaccgaa ataatcttta tcggtcactt tatcgcccac tgccgccagt tttgggttat
    20761 ctttcagcca ctcagtgacc actgcggtgt caccaaatac gctgtcgata cgcccgtttt
    20821 gcagatccag ttttgcgttc tggtagctgt catacggaac ggtggtgatt tccgggtgct
    20881 tatccataat gaacttctgg tgtgtcgtcc cgttctgaac gccgactttt ttgcctttca
    20941 gctgatcaac actggtgtat ttgccttgct gacccacaaa cagggcagag ttgtcatagt
    21001 acggggtagt aaacagtacc tgcttttcac gctccggagt gatatccatg cccgccatca
    21061 ctgcttctac gcgacggaat ttcaggcttg ggatcaggct gtcaaacgcc tggttagaga
    21121 aagtacaggt tgcatcaatc tctttacaca gtgcctgcgc caggtcgacg tcaaaaccaa
    21181 cgatctggtt gtttgcatca atcgattcaa acggaggata gggggcttcg gtagcaaaac
    21241 gaatggtttc ggcagctgtg gcggaaagac taaaacctgc aattaacgcg gcaatcagaa
    21301 cttttttcat tgttgttatc ccgaatctta gtgagagaga tagtttttaa atgcttcggt
    21361 ttgcggctcg gtaaagcagc tcgcgtcgcc ttgttctacg atatgaccat tttccatata
    21421 caccactcgg ctggcggttt tacgcgccac ttcaacttcg tgggtgacga tcacctgggt
    21481 aatattcgtt tctgccagct cacgaatgat gctgacgatt tgtgccgtaa tttccgggtc
    21541 cagtgcggcg gtcggttcat cgaacagcag tacctgcggt tccatcatca acgcacgggc
    21601 aatagcaaca cgctgctgct gaccaccaga aagatgcagc gggtaacgat cgctataagg
    21661 tttgagacgc agacgttcca gcagtttttc tgcacgggcc agcgcctgat ctttactcaa
    21721 ccccagtaca cggcagggcg cttcaatcag gttttgctgc acggtcagat gcggccacag
    21781 gttgtattgc tgaaacacca tgccaacgtt acgacgcaaa tcgcgaatcg ctttgtcaga
    21841 gggtgttttg gtgaaatcga aatggttgcc tgcaatgttg agcgtaccgg agcgcggcat
    21901 ctcaagcaga ttgagtacac gcagcagcga gcttttaccc gcgccgctgg ggccaagtaa
    21961 caccagcgtt tcgccctgtg ggcaatccag cgtgatatcg aacagcgcct gatgcgcgcc
    22021 gtagaagcaa ttaatgccgt ttaattgaat actcattgac actcgtatac tggcagtctg
    22081 atagctattg aggtcgaaga tagtaccttt aacagaataa ttatgcaata tttatgcttt
    22141 aaaagttaaa agcaaagcgc attattcaat aaacatagca caaaataacg ggggcggtgg
    22201 tcggtgagca taaatgtcgg cattcctcac gaaatgccgg ataatttacg gggtttattg
    22261 gttgatcaag gcgttagcga ttctcgatgg actgacggag cgtacccgcc gtggcatgaa
    22321 cgctaccgcc taagtagcgc acatcgtcga tgacccaaca ctggccttcc tgaatcatta
    22381 acacttcatc ctgccagccc tggtcaccct gtttgagatc cacccgcaat ggaatgttac
    22441 gggcatcacg attagggata gtcgatgcac tggcaacgtg ggcgctgtct ggcaaggtgg
    22501 ttcgactgga gaacggatcg ctgcttaaca attcacgatg actattatcg cggctggcat
    22561 cgctaagttg ggttgccagt ttgtcactca aataggggcg cagggcggta atgtcattgc
    22621 tgcgatgttg aatgcggtag tcgtagaatt gttgagccac actgtcaggc cctccctgga
    22681 cacaggaacc ggtgcggggg ccgttatctt tataagctgg cgtgacggtg gtacaggcac
    22741 tcagcagcag tgcacatgga ataagtagag atagtgaacg gtagcgcata atcatttcct
    22801 tattagcttg ctgtctgcgt cacactacga ccaaacgcta tcagattatt taatacattg
    22861 tttttaatac aattctgaaa caatctgaac acctgattgg gtttgagtcc attgctggcg
    22921 acccactcaa ggttgctcgt agctgactta cttaaattac ccaaacaata gacagtgtaa
    22981 accatttgtg aggttgagcg tgttatatgg ctgaaatgat catttaaggt aaatatgata
    23041 gttctgaaat ggtggtataa atcctcctgt agaggttaag agcatctgca agaagccagt
    23101 ttcacattta taacgcgtga taaatgaatg cggttattgt ctaatcatta caaaattttt
    23161 cacatgcgat aagagatcct gcgattaaga tgattgccaa aattacgttg tatttttgaa
    23221 aatgagcgaa atttgatagt cccagctttg actctcgata atattattta tgctacaatt
    23281 gtgggtaaag aatttaagct aatgaatttg agtgaagaat aaattaaa
//
LOCUS       DAZPAB010000045        22971 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZPAB010000045 DAZPAB010000000
VERSION     DAZPAB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22971
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.045"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..1032)
                     /locus_tag="ACHUPG_001582"
     CDS             complement(<1..1032)
                     /locus_tag="ACHUPG_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR0861246.1"
                     /translation="MKLRGGTWHCDFVAPDGSRVRRSLETSDKRQAQELHDRLKAEAW
                     RVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWMLHFREAILSDITTEKIM
                     EAVDGMENRRHRLNWEMSRDRCLRLGNPVPEYKPKLASKGTKTRHLAILRAILNMAVE
                     WGWLDRAPKISTPRVKNGRIRWLTEEESKRLFAEIAPHFFPVVMFAITTGLRRSNVTD
                     LEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQGLHKRWVFVHTKPAYR
                     SDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWASWLVQSGVSLLALKEM
                     GGWETLEMVQ"
     gene            complement(915..1262)
                     /locus_tag="ACHUPG_001583"
     CDS             complement(915..1262)
                     /locus_tag="ACHUPG_001583"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0861247.1"
                     /translation="MEEEIFTREEAASYLKVDKGTITQWIRSGRLQAAKINPDKPKSP
                     YRICKSDCIAALKSVRHNSAVNAVDVQEVKACQSNYAVARGTAISSRQMDQELDALLK
                     HRTKGKRKNFTIV"
     gene            complement(1349..1693)
                     /locus_tag="ACHUPG_001584"
     CDS             complement(1349..1693)
                     /locus_tag="ACHUPG_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861248.1"
                     /translation="MSDLAMKVLKWQSTDDVGISSATLASIACGLKKNIYGHSFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            complement(1794..1973)
                     /locus_tag="ACHUPG_001585"
     CDS             complement(1794..1973)
                     /locus_tag="ACHUPG_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eag protein"
                     /protein_id="HFR0861249.1"
                     /translation="MSCPKCGSGNIAKEKTMRGWSGDYVCCDCGYNDSKDAFGERGKN
                     EFVKINKEREGNEKS"
     gene            complement(2070..2999)
                     /locus_tag="ACHUPG_001586"
     CDS             complement(2070..2999)
                     /locus_tag="ACHUPG_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR0861250.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVAY
                     IIQDSDARSRGEKGILRYFANISDEDINEYEITVTPLYAAPPETVSVPDAMEMDDDFD
                     SAFEHGKAVGWNAYRAAMLQSGNFRENKNSSTNNFREIAETSTNYPVIPSEVLSAIQK
                     VAKIRADFDDFDGDRRGIGDCLDEAEQELIVTINKYASQLAAEPIAPNDVREQTAIPQ
                     VPVTPDGWISCSERMPVIGELNWRTSFPLLITCEIGVIPAYYGFVSVNGNKHYGFMES
                     LKYGDDNGNHPQTNEYGLISNVTHWMPLPEPPL"
     gene            complement(3002..3193)
                     /locus_tag="ACHUPG_001587"
     CDS             complement(3002..3193)
                     /locus_tag="ACHUPG_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861251.1"
                     /translation="MDGQISIVRPGACDDREIRLIIRLAMGKTITALITPENLALALT
                     GKSDLPVELKLRNIEIKVK"
     gene            complement(3195..3824)
                     /locus_tag="ACHUPG_001588"
     CDS             complement(3195..3824)
                     /locus_tag="ACHUPG_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFR0861252.1"
                     /translation="MSEINYQALREAAERAIPAMERLLMLPADDLLSEQELKDYGVDI
                     DALNAFKFLAGPETVLALLDERVRNQQYIKSRDQENEDIALTVGKLLIENGRLVADTL
                     RHLADNEIDSDYFAITSTNENGTEIDHEMAITDYALQAAGTVDELVAALESAENRIAE
                     LGERGILLPERSSMLHRTDFHDDYQTVMAYKVSEVIAAIRAAGIRIKGE"
     gene            complement(3821..4396)
                     /locus_tag="ACHUPG_001589"
     CDS             complement(3821..4396)
                     /locus_tag="ACHUPG_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001701210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hNH endonuclease"
                     /protein_id="HFR0861253.1"
                     /translation="MKQMSLIEMDGFLKGKCIPCDLKVNETNAEYLVRKFAEAEAKIS
                     ALYEDHQKAIESIKQADAAVKLAHEKFSALAAENAGMKSVIEYCINPDNQPEYHDQGM
                     GCGVEDHGYQRDGYSACYYGWESAMDRVYSEVIPDAIPETPATDTFLDEVRAQGVEMF
                     AASMKVVGGHEHPYSAMANEFAAQLRKGGGQ"
     gene            complement(4393..4557)
                     /locus_tag="ACHUPG_001590"
     CDS             complement(4393..4557)
                     /locus_tag="ACHUPG_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFR0861254.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRKSFGSVNDNKY
                     LNAMWRSGKK"
     gene            complement(4568..4861)
                     /locus_tag="ACHUPG_001591"
     CDS             complement(4568..4861)
                     /locus_tag="ACHUPG_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HFR0861255.1"
                     /translation="MPAPLYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEELERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(4885..5268)
                     /locus_tag="ACHUPG_001592"
     CDS             complement(4885..5268)
                     /locus_tag="ACHUPG_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861256.1"
                     /translation="MAHSITVRLNKPAREFQAGENIGFNIRAGVQYYDRQTKKKEWTN
                     YSAVVFAKPGAQADYYRSVLVEGGIVEITGENIRVDVYQGQNGQSITLELLNAKIGFA
                     TSGNSQQQQSSNHQNHPEYDDSIPF"
     gene            complement(5268..5873)
                     /locus_tag="ACHUPG_001593"
     CDS             complement(5268..5873)
                     /locus_tag="ACHUPG_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFR0861257.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAALQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDIYNIRRDELEGAAA"
     gene            complement(6130..6282)
                     /gene="kil"
                     /locus_tag="ACHUPG_001594"
     CDS             complement(6130..6282)
                     /gene="kil"
                     /locus_tag="ACHUPG_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFR0861258.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINI
                     PWYRRK"
     gene            complement(6267..6401)
                     /locus_tag="ACHUPG_001595"
     CDS             complement(6267..6401)
                     /locus_tag="ACHUPG_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861259.1"
                     /translation="MMHFQLAGSGVMSAFYPHESELSRRVKQLISAAKKQLEALCAMK
                     "
     gene            complement(6596..7066)
                     /locus_tag="ACHUPG_001596"
     CDS             complement(6596..7066)
                     /locus_tag="ACHUPG_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861260.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQKRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSTPDIEYNFGHNQETENWVFY"
     gene            complement(7075..7401)
                     /locus_tag="ACHUPG_001597"
     CDS             complement(7075..7401)
                     /locus_tag="ACHUPG_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFR0861261.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRIERKNQRTWYSKPRSEMGVTCVGRQKMKL
                     GSKPLI"
     gene            complement(7882..8076)
                     /locus_tag="ACHUPG_001598"
     CDS             complement(7882..8076)
                     /locus_tag="ACHUPG_001598"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861262.1"
                     /translation="MNGDRYEFTKDSTEDSVFHVTINGDKSSVYESVSGVYPEMKYTA
                     LSSNTMVGEYQSGGGITVET"
     gene            complement(8166..8798)
                     /locus_tag="ACHUPG_001599"
     CDS             complement(8166..8798)
                     /locus_tag="ACHUPG_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFR0861263.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            8902..9117
                     /locus_tag="ACHUPG_001600"
     CDS             8902..9117
                     /locus_tag="ACHUPG_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0861264.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            9234..9515
                     /locus_tag="ACHUPG_001601"
     CDS             9234..9515
                     /locus_tag="ACHUPG_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015995142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFR0861265.1"
                     /translation="MGTIATKSKKAARIESALLNKLALMGQKTFARAMGVPEYQVSRW
                     KNGFFSQVSMMLAVLEYGIEDDEMAELTKRLADYLTKEKAPKNGEFFEA"
     gene            9550..9711
                     /locus_tag="ACHUPG_001602"
     CDS             9550..9711
                     /locus_tag="ACHUPG_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861266.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            9698..10528
                     /locus_tag="ACHUPG_001603"
     CDS             9698..10528
                     /locus_tag="ACHUPG_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFR0861267.1"
                     /translation="MGVVKLADYRPQLEVVEHRVAELEDGYTRTANTLLEAAMLSGLT
                     LHQLLIVMAVWRKTYGYNKKIDWIGNEQFAELTGMAPTKCSTAKNELIRMGVLIQVGR
                     QVGMNTNISEWKTKVNGFGKTFTNSVKQTFTKSVKSNLPNQSNTKDNIQKTINTNTPL
                     PPNGGGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQL
                     KTPNVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA
                     "
     gene            10525..11901
                     /locus_tag="ACHUPG_001604"
     CDS             10525..11901
                     /locus_tag="ACHUPG_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR0861268.1"
                     /translation="MKQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIDELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTASNAPAARGR
                     RYAQGADV"
     gene            11974..12300
                     /locus_tag="ACHUPG_001605"
     CDS             11974..12300
                     /locus_tag="ACHUPG_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861269.1"
                     /translation="MADWQIPIIILAGASLVAGFILLKKHKDRDQKVEVLYGYPANST
                     TWLTIYHYRKSGRWVFEWDDLFAEKRPKSWGDISECMMFEERKSGATREEFNEAWARL
                     SERGYL"
     gene            12297..12497
                     /locus_tag="ACHUPG_001606"
     CDS             12297..12497
                     /locus_tag="ACHUPG_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861270.1"
                     /translation="MSKYEKLDQNILSMLSERPTPVFDIWLKWRSNGMYIETIDRRMQ
                     YLRKKGLVANVRGKGWVKINLS"
     gene            12509..12697
                     /locus_tag="ACHUPG_001607"
     CDS             12509..12697
                     /locus_tag="ACHUPG_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004904153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861271.1"
                     /translation="MDDSLEIFEEWCADELGVSAYFIRQMRSKNILGVIEYKRVEINK
                     RYRAWMAAVRAAGVKVKE"
     gene            12700..12864
                     /locus_tag="ACHUPG_001608"
     CDS             12700..12864
                     /locus_tag="ACHUPG_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861272.1"
                     /translation="MSAYEEIMLALRFFFGVEEDENVNEIIGQDHDPIGTIAAALDDY
                     RSVNGEETNV"
     gene            12845..13285
                     /locus_tag="ACHUPG_001609"
     CDS             12845..13285
                     /locus_tag="ACHUPG_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFR0861273.1"
                     /translation="MRKLTFELRSPIHQQNAIQAIQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGRSTS
                     RMRVGEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDKTE"
     gene            13282..13872
                     /locus_tag="ACHUPG_001610"
     CDS             13282..13872
                     /locus_tag="ACHUPG_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFR0861274.1"
                     /translation="MNKYRLIYADPPWQYRDKANDGNRGAGHKYDVMNVQDICRLPVW
                     DLADPESCLLAMWWVPTQPAEALKVIEAWGFRLMTMKGFTWHKTNKHKGNSAIGMGHM
                     TRANSEDCLFAVRGRLPERMDASICQHFTAPRMEHSAKPPIVRDMLAKLLGDVPRCEL
                     FSRDKVTGWDMWGNQCDSDFELAPGMAIKPCKMVIA"
     gene            13869..14042
                     /gene="ninD"
                     /locus_tag="ACHUPG_001611"
     CDS             13869..14042
                     /gene="ninD"
                     /locus_tag="ACHUPG_001611"
                     /inference="COORDINATES: protein motif:HMM:NF036649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinD"
                     /protein_id="HFR0861275.1"
                     /translation="MKHCYRCGERKEDDRFRPGQPYWNRWCLRCERTPTGVLPLPQEK
                     EDVWRDSDEVSPT"
     gene            14009..14184
                     /locus_tag="ACHUPG_001612"
                     /pseudo
     CDS             14009..14184
                     /locus_tag="ACHUPG_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
     gene            14187..14588
                     /locus_tag="ACHUPG_001613"
     CDS             14187..14588
                     /locus_tag="ACHUPG_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861276.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMMSIRLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVENHHAVRQAKRKANMTYMKAYRKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
     gene            14548..14778
                     /locus_tag="ACHUPG_001614"
     CDS             14548..14778
                     /locus_tag="ACHUPG_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFR0861277.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEHCCAELMSDPNSSMYEEEDDGQTSAKTM"
     gene            14750..15361
                     /locus_tag="ACHUPG_001615"
     CDS             14750..15361
                     /locus_tag="ACHUPG_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFR0861278.1"
                     /translation="MAKPARRRCKNEECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIG
                     QEAVDEIESNHNRHRWTVVECKAIKAEYQQKLKDLRNSRSEAA"
     gene            15358..15564
                     /locus_tag="ACHUPG_001616"
     CDS             15358..15564
                     /locus_tag="ACHUPG_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFR0861279.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            15542..16207
                     /locus_tag="ACHUPG_001617"
     CDS             15542..16207
                     /locus_tag="ACHUPG_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFR0861280.1"
                     /translation="MRYYEKIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NIDYDKEILAKALSHKADELPLIIELVSKDKKYVICHADYPCDEYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            16204..16827
                     /locus_tag="ACHUPG_001618"
     CDS             16204..16827
                     /locus_tag="ACHUPG_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0861281.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            17260..17583
                     /locus_tag="ACHUPG_001619"
     CDS             17260..17583
                     /locus_tag="ACHUPG_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFR0861282.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            17567..18043
                     /locus_tag="ACHUPG_001620"
     CDS             17567..18043
                     /locus_tag="ACHUPG_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFR0861283.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            18040..18477
                     /locus_tag="ACHUPG_001621"
     CDS             18040..18477
                     /locus_tag="ACHUPG_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR0861284.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKEQRDKAASTI
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLK"
     gene            18682..19224
                     /locus_tag="ACHUPG_001622"
     CDS             18682..19224
                     /locus_tag="ACHUPG_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR0861285.1"
                     /translation="MNYPTVVNDIDFRDLIFVANNDPVTDSFMVAKAFGKLPKNVVRD
                     IERTIEACPPEFDTKLNFELCYKNNELQNGKPQKFYRLRKDGLMLLVMSDTKKEAMRI
                     KIAYINAFNWMYAMLQVGHRQFEEERNAVMLEYMKEKDVASMSGRLLNRWGKIKKPQL
                     MARIERLEQHGQTVIPGLIN"
     gene            19444..19659
                     /locus_tag="ACHUPG_001623"
     CDS             19444..19659
                     /locus_tag="ACHUPG_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR0861286.1"
                     /translation="MDDNGNQWVNVEHRLPESKEGMWSKEVIALTDTGDVFKLSCMGS
                     YWQRTKAFIDSGASKVTHWMPLNYPDD"
     gene            19807..20049
                     /locus_tag="ACHUPG_001624"
     CDS             19807..20049
                     /locus_tag="ACHUPG_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HFR0861287.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITIRTFEAVRKSKEALDLFTTGA"
     gene            20085..20573
                     /locus_tag="ACHUPG_001625"
     CDS             20085..20573
                     /locus_tag="ACHUPG_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFR0861288.1"
                     /translation="MAAPKGNRFWEARSSHGRNPKFESPEALWAACCEYFEWADDNPL
                     WEGKVFSYQGEIIKANVPKMRAMTISGLCTFLDITRQTWGTFRSMEGFSDVTSRAEDI
                     IYDQKFSGAAADLLNANIIARDLGLKEQSQVEDVTPDKGDRDKRRSRIKELFNRGTGR
                     DS"
     gene            20551..22050
                     /locus_tag="ACHUPG_001626"
     CDS             20551..22050
                     /locus_tag="ACHUPG_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR0861289.1"
                     /translation="MELDAILDNLSDEEQIELLELLEEEENYRNTHLLYEFTPYSKQR
                     EFIDAGHDYPERCFMAGNQLGKSFTGAAEVAFHLTGRYPGTKGYPADGKYGGEWKGKR
                     FYEPVVFWIGGETNETVTKTTQRILCGRIEENGEPGYGSIPKEDIISWKKSPFFPNLV
                     DHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGVWFDEEPPYSIYGEGLTRT
                     NKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYDAEHYTDEQKEQIIASYP
                     EHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVIGGMDFGWDHPQAQVQLW
                     WDKDADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTAWPHDGNQHEKGGGEQLKGQY
                     ADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFKVFNTCEPFFEEFRLYHRDEN
                     GKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKIPAPIRPIARRT"
     gene            22051..>22971
                     /locus_tag="ACHUPG_001627"
     CDS             22051..>22971
                     /locus_tag="ACHUPG_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFR0861290.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEW"
BASE COUNT         6235 a         5367 c         5546 g         5823 t
ORIGIN      
        1 ttgaaccatt tcgagagttt cccatcctcc catctcttta agtgcaagaa gagagacacc
       61 ggactgaacc agccagcttg cccaggtatg cctcagatca tggaagcgga agttgctaat
      121 gcctgcccgc tttaacgctc ccttccatgc cttgttgctg tcggttctca tcttccttac
      181 cgctgctgtt tttgttccgt cgcttcggta ggcaggtttg gtgtggacaa atacccatct
      241 cttatggagc ccctgctgtt ttcttaatat ctggcatgcg gtttcgttaa gaggaactcc
      301 gatcgcattg ccagcttttg tttcatcagg gtgcatccat gccattttct tatccagatc
      361 gacctgtgac cactcaaggt ctgtaacgtt ggaacggcga aggcctgtcg tgattgcaaa
      421 catgaccaca gggaagaagt gaggagcaat ttctgcaaac aggcgcttcg attcctcctc
      481 tgtaagccat ctgatgcgtc cattcttaac gcgtggtgtt gatattttgg gtgccctgtc
      541 aagccatccc cattcaacag ccatattgag aatggcgcga agtattgcca gatgcctcgt
      601 cttcgttcct ttgcttgcaa gctttggttt atactccggc accggattgc caagtcgcaa
      661 acacctgtcc cggctcatct cccagttcag gcgatggcgg cggttttcca tcccgtctac
      721 cgcctccatt attttttctg ttgttatgtc agagagaatg gcttctctga agtgcaacat
      781 ccagaacgat ataatgctct tgtcatcatc aatggacttc ttatccgatt tctcacgcag
      841 ccaccgtatg caggcttcct tgaatagctt tttcggtgat tccccgagat tttttactct
      901 ccacgcttct gctttcagac gatcgtgaag ttcttgcgct tgccttttgt ccgatgtttc
      961 aagagagcgt ctaactcttg atccatctgg cgcgacgaaa tcgcagtgcc acgtgccacc
     1021 gcgtagtttg attgacatgc tttaacctcc tgcacatcaa ccgcattcac cgcgctattg
     1081 tgtctcacag acttaagcgc cgcaatgcag tctgacttgc aaatgcgata tgggctttta
     1141 ggtttatctg gatttatctt tgcggcctga agtcgtccac ttcgtatcca ctgcgtgata
     1201 gtgcctttgt ctaccttcag atacgacgct gcctcttcac gagtgaagat ttcttcttcc
     1261 acctggaatc tccatttatt ggattggtat tattgcggta ggtctggata tcattgagca
     1321 atgaacaggc ctcatcgagt gtgaggcgtt agtccttgcg tagctcgctg attcttctgt
     1381 aagtctctgg tgctttgttc ccgtacgtct tcatttcaga cttcaacaga gcaacgagtg
     1441 aatcccattc gttgaggatt cctttgaatg ccggaacgcg ctttgcaacc ttgtcgaatg
     1501 aatctctgat ttctggaatc tgctcaacaa gtgcaacgca tcgccggaaa tcggctgcgt
     1561 catgtggagc accgaagcta tgaccataga tattcttttt cagtccacat gcgattgagg
     1621 caagagttgc gctactgatg ccgacatcgt cagtcgattg ccatttcaaa accttcatag
     1681 ccaaatctga catttcttgt ctccaataaa aaaccgccat caggcggctt ggtgttcttt
     1741 caattcttca attcgaatat tggttacatt gttttcatat atgaataaat aaattagctt
     1801 ttttcgttgc cttcgcgttc tttattaatt ttgacaaact cgtttttacc acgctctcca
     1861 aatgcgtctt tagagtcgtt gtatccgcaa tcgcagcaca cataatcacc agaccatcca
     1921 cgcattgttt tttcttttgc aatatttcca gaaccgcatt ttggacaaga catatcacta
     1981 cctccaaagc atgagtgaga tgacaacgta acattgattg gagattaaca atagattgct
     2041 gatgtaaaag atatgtataa gcttcgcttt caaagtggag gctctggtag cggcatccag
     2101 tgggttacat tgctaatcag accatattca ttagtttgag gatggttgcc gttatcgtct
     2161 ccgtatttaa gactctccat aaaaccataa tgcttattcc cattaacgct cacaaagccg
     2221 taataagctg gtataacgcc gatctcacac gtaatcagca aaggaaaact agttctccaa
     2281 tttagctcgc cgattacagg cattctctca ctacagctta tccaaccatc cggagttacc
     2341 ggaacttgcg gaatggctgt ctgctctcga acgtcattag gcgcgatagg ttctgctgcc
     2401 aactgactgg catatttgtt aatggtaacg ataagctctt gctcagcctc atccagacaa
     2461 tcaccgatac ctcgcctgtc accgtcaaaa tcatcgaaat cggcacgaat cttggcaacc
     2521 ttctggattg cggacaacac ctcactagga attaccggat agttggttga cgtttccgcg
     2581 atttcacgaa aattattggt tgacgaattc ttgttttccc gaaagtttcc ggactgaagc
     2641 atggctgcgc gataggcgtt ccagccgaca gcttttccgt gttcaaacgc gctgtcaaag
     2701 tcatcatcca tttccatcgc atcaggcaca gataccgtct ctggaggggc ggcgtaaagc
     2761 ggagttacgg taatttcgta ctcgtttata tcctcgtcgc tgatatttgc gaaatagcga
     2821 aggatgcctt tctctccacg actacgagcg tcagagtcct gaatgatata cgccacaggc
     2881 tctgcttcca gcgatgccag cgcaattcgt gccagttcca tttgttcgcc acgagtaagc
     2941 ccgttttcaa gcggattttt aatgaacaat tcaatacgtt ctttggtaat agtggtcata
     3001 gctatttcac cttaatctca atatttcgca gctttagctc tactggcagg tctgactttc
     3061 ctgttaacgc taatgcgaga ttttctggag taatgagagc agttattgtt ttccccatcg
     3121 ccagacgaat aatcagtcgt atctcgcgat cgtcacatgc tcctggtcga acaattgata
     3181 tttgtccgtc catctcactc tcctttgatg cgaatgccag cggcgcggat tgcagcgatg
     3241 acctcagaaa ctttgtatgc cattaccgtt tggtaatcat cgtgaaaatc tgttcgatga
     3301 agcatgctgc tacgttccgg gagcagtatt ccccgctccc ccagttctgc tatgcgattc
     3361 tctgcggatt ccagcgccgc aaccaattcg tctacagttc cggcagcttg cagtgcgtaa
     3421 tcggtaatag ccatctcatg atcaatttca gtaccgttct cattcgttga ggtgatagca
     3481 aaataatcag agtcgatttc gttatcagct aagtggcgta gcgtatcggc aacaagccgg
     3541 ccgttttcga ttagcagctt ccctaccgtt agcgcaatat cctcgttctc ctggtcgcgg
     3601 gatttgatgt attgctgatt ccttacccgt tcatccagta gtgccagcac ggtttctggt
     3661 ccggccagaa atttgaaggc gttgagcgca tcaatatcca caccgtaatc tttaagttcc
     3721 tgttcactta acaaatcatc agctggcaac attaacaggc gttccattgc tggaattgca
     3781 cgttccgccg cctcacgcag tgcctgatag ttaatttcgc tcactggccg cctcctttgc
     3841 gaagctgggc ggcgaactcg ttagccatcg ctgaatatgg gtgctcatga ccgccaacaa
     3901 ccttcatgct ggcagcaaac atctccacgc cctgcgcccg cacttcatcc aggaaagtgt
     3961 cggtggctgg ggtttctgga atggcgtctg gaataacctc tgagtagacg cgatccattg
     4021 ccgactccca tccgtagtaa caagccgaat agccgtcgcg ctggtagccg tggtcttcaa
     4081 ctccacatcc catgccctgg tcatggtatt caggctgatt gtcgggatta atgcagtatt
     4141 caatgaccga cttcatcccc gcattctccg ccgccagcgc cgaaaacttc tcgtgtgcca
     4201 acttaacagc cgcatcagcc tgcttaattg actcaatcgc tttctggtgg tcttcgtaca
     4261 gagccgaaat cttggcctcc gcttcagcaa atttacgcac cagatattca gcgtttgttt
     4321 cgttaacctt taaatcacat gggatgcatt tacctttcag aaaaccatcc atctcaatta
     4381 gtgacatttg tttcatttct tcccactccg ccacatagca ttcagatatt tgttgtcatt
     4441 aacagaaccg aaactctttc tcttaagtaa ttcctctctc gatggcattg gctttacgcg
     4501 ttggcgaata atcatttctg ccggaagaat gccgggattg tatgcaagtc ctctcatggt
     4561 aaattcctca gtcattactg atagcgccat agcgtgagcg gtaattacgc aggcgcgggt
     4621 caatttcagg gaagtgggta tatgtggctt tgcggaatgg tcggattgat gtctggtaaa
     4681 ttcgctcgag ttcttctttc tctgcaagcc atatacagtg gcgaaattcc ttttcctctt
     4741 tcgtttcctg cggtagcgac attatccgat cgtagttttt tctgaattta tccagcacct
     4801 ccgatacgga attgccggaa cagcggcgtg ggtcatccgc accatacaga ggcgctggca
     4861 taatggaatc cttatgttgc tactttagaa gggaattgaa tcgtcgtatt caggatgatt
     4921 ttgatgattg ctactttgct gctgttggct gtttcctgaa gttgcaaatc caatctttgc
     4981 attcagtaat tcaagagtga ttgattgtcc attttgcccc tgataaacat caaccctgat
     5041 gttttctccg gtaatttcca caatgccacc ttcaacaaga acactacggt agtaatccgc
     5101 ttgcgctccc ggcttggcaa atacaacggc gctgtagttt gtccattctt tcttttttgt
     5161 ctggcgatcg taatactgaa cgccagcacg gatgttgaat ccgatatttt ccccggcctg
     5221 aaactctctt gcgggcttgt ttagtcttac agtaatcgaa tgtgccatta agcagccgct
     5281 ccttctaatt cgtctcgtct gatgttgtaa atgtcctgcg ctttgtgctg ctccggtgtg
     5341 ccttcgagca tcttccacgc tttggcgaac gcctgtttaa gctcttccac ggtgtttttc
     5401 tgcaatgctg cgtcagtgaa tgcttttaga acctgttcag gtgtaggtga tggttttgat
     5461 tgctttgctg ctgcgttctg ctgatgttta tgctcgtcgg tatctgcatc tttcgcatca
     5521 tcaatgccga acaaaccatt gaggcaatac ttgcgtgcat aagagcttgt ggctcccgta
     5581 acttgtgcag aatccattcc tttcttgctt tcttcctctc gtgcaagagc ggttgccgta
     5641 tgactgtttt cgccatcggt aatagttgcc gtggctttca cgtaataccg atcaccaatc
     5701 aacacaactt catcgctgat tgataaaaac agaccattca gtaacggctt aacgccttca
     5761 agaatgtctt cgcagcttct gtatttatat ttaccgaatg agttgtactg gttctttggc
     5821 gcgttcaagt tctcctgaat agctgccagt cttgcgtaaa attctttgct catatgtttg
     5881 ttctcagaat gggcatggcc caaggaaata acgctgattt aatacttcga ctcgggacaa
     5941 attaaggcat acccgcattc cttcgcggtc gccattatgg cgataccaga gagctttctg
     6001 cgtgtacatg cgtctctgta acttgctctc cttcactgtg gttgcaagtg acatgaatat
     6061 ctccttcgtt accgattaat tctttcatct gacgaatgaa ttcttcgtct gaccagttat
     6121 ctgtaaaact catttcctgc gataccacgg aatattgata gctgatttca tcgctttatt
     6181 tgcttcaagc cacatttttg aatcaccaat aaatctggct attactgctt tgttttgtgc
     6241 tgcacgaagc atctggtgat tgatggctat ttcattgcgc ataacgcctc cagttgtttc
     6301 tttgctgcac tgattaattg tttaactcgg cgtgataatt cagattcgtg cgggtagaaa
     6361 gcggacatga cgccgctacc cgcgagctga aagtgcatca tggataactc cttctgtttg
     6421 attgcataac gaaaacgcct cgagtgaagc gttattggta tgcatataaa aaagccctca
     6481 tactggaggg caaagaagat ttcaaataat cagaacaagt cggctcctgt ttagttacga
     6541 gcgacattgc tccgtgtatt cactcgttgg aatgaataca cagtgcttac tcgtactaat
     6601 aaaataccca attttctgtt tcttggttgt gcccaaagtt atattcaata tctggtgttg
     6661 atgtatcaat attcttcatc ccatcaacaa gagttgatac aacagccaaa tcctgtttga
     6721 ttctcattaa atggtatttc ttccggcgca ataaactttc aatggcaagt ttcttcgttg
     6781 ggaatgcaaa agatctttct gcattttttg ctactttctt aattgcatat ctatttctct
     6841 tttgtttcca ttcctgtaac cactgatttg gtgctggttt aaaattaaca atccaatgcg
     6901 caggaaccaa ccatgcataa tgctctgtct gatgaaaagc tatatattga agtgcgaata
     6961 ttttgattcc atcttcttca actgtcgcct ggaatctcca gaaaacaggc attccatcat
     7021 gttcagtttc tgattcagga aaaggtacgc tccatgattt tgtcatatct cacctcaaat
     7081 aagtggcttg ctgcctaatt tcattttctg gcgaccaaca caagtcacac ccatttcact
     7141 gcgtggcttg ctgtaccatg tgcgctgatt cttgcgctca atacgttgca ggttgctttc
     7201 aatctgttcg tggtattcag ccagcaccgt aaggtctatc ggattcagtg cgctttctac
     7261 tcgtgatttc ggtttgcgat tcagcgagag aatagggcgg ttaactggtt ttgcgcttac
     7321 cccaaccaac aggggatttg ctgctttcca ttgagcctgt ttctctgcgc gacgttcgcg
     7381 gcggcgtgtt tgtgcatcca tcttgattct cctgtcagtt agctttggtg gtgtggtagg
     7441 tgggagaccc atttcgacct gcttcggccg acttcaattc ggcaatagtt ccgcaggcct
     7501 cgccgcttta cgtgcgacat attcccatcc atgaaccctt caccacaccc caaagccttc
     7561 tgctttgaat gctgcccttc ttcagggctt aatttttaag agcctcacct tcatggtggt
     7621 cagtgcgtcc tgctgatggc ttaaaattac aagaaagatt gtatgttgta aacaagaaat
     7681 attgtaaaaa ggggcgtaaa aacaaactcc attgttttta aatgaaaaat agtttgtttt
     7741 tggttatcga gattgaggtg gggattactg gttgcaggtt ccgactacat caccaacaaa
     7801 tgatttggtt gatgtaagtt gttgcatacc tgggatattc attactttgg agtaaagagc
     7861 ttttttgtct gtagtgattg atcaagtttc aacggttatt cctcctccag actggtattc
     7921 tcctaccata gtgttcgatg acaaagcagt gtatttcatc tctggataga cgccagaaac
     7981 tgattcataa actgatgatt tatcgccatt tattgttacg tggaaaacgg aatcttccgt
     8041 gctgtctttt gtaaactcgt aacgatcgcc attcattgac ccgtacccgt gcaggtttgt
     8101 gacaatccag cattcagaat tggcgctggt agttaagagt attgagagta gcgccgcaat
     8161 cctgatcata cgaattttac cctcgcttcc acgacaacac cgataatctt gcagttcccg
     8221 ttgataggag tcataggcca tgaagggttc aggcctttca ggtacttctg cccgccatct
     8281 ataaccagtt tcttgaatgt tgcttcgttc gcgtcagtca gtttggctac aacaaggctt
     8341 ccattcactg gctcgcgtcc agtatctact aacaccatat gaccttcagg gatgctttga
     8401 cctacaggtg aggtcatgga atcaccttca accttcagcc agaatccatc gcctaataag
     8461 ttaacgtcac tgtcatacca ttcatcaatg tccttgatat cgtagggttc acaagcttca
     8521 caccacgaac cagctctaac catgctaatc aatggatatt tccctttggg ctcaacatgc
     8581 ccaacaaatc taacattcga atcagaggtg ccattgagca gccagtcaac acttacgcca
     8641 agagctgacg caagttctgg taaaaagcgt ggtcgcttag ttttaccgtt ttcgagctgc
     8701 tctatagact gctgggtagt ccccaccttt tgagcaagtt cagcctggtt aagtccaagc
     8761 tgaattcttt tgctttttac cctggaagaa atactcataa gccacctctg ttatttacct
     8821 ccaatcttca caagaaaaac tgtatttgac aaacaagata cattgtatga aaatacaaga
     8881 aagtttgttg atggaggcga tatgcaaact ctttctgaac gcctcaagaa gaggcgaatt
     8941 gcgttaaaaa tgacgcaaac cgaactggca accaaagccg gtgttaaaca gcaatcaatt
     9001 caactgattg aagctggagt aaccaagcga ccgcgcttct tgtttgagat tgctatggcg
     9061 cttaactgtg atccggtttg gttacagtac ggaactaaac gcggtaaagc cgcttaagac
     9121 attcccgctc ttacacatcc cagccctgaa aaagggcatt accagaaaca aatctctatg
     9181 gttttgcgtt tctttgcgaa gccaactcta tctaatcatt aaggaaatta tctatgggta
     9241 ctattgcaac taaaagcaag aaagcggctc gcatcgagtc agccttgctg aacaaactgg
     9301 cactgatggg gcagaagaca ttcgctcgag caatgggggt tcctgaatat caggtaagcc
     9361 gatggaagaa tggtttcttc tcgcaggtaa gcatgatgct tgctgttctg gaatacggaa
     9421 tcgaagacga tgaaatggct gaattgacta agcggcttgc cgattacctg acaaaagaaa
     9481 aagccccgaa gaacggcgaa ttcttcgagg cctaatgtag aaagactgga tcaatccacg
     9541 ggagtaatta tgacaaaacg tcgtaagaaa taccaggaaa aagaagagat tcgacaccct
     9601 gattcacctg agggattagt ggtagccgca gcaaataaca gggcgttcgc agagcgcctt
     9661 gttggtgttt acagactagc caaagcagga gtgaaacatg ggcgtcgtta agttagctga
     9721 ttacaggcct caactggagg tcgtggagca tcgcgtggca gaactcgaag atggctacac
     9781 tcggactgca aacacactgt tagaagccgc catgctttct ggacttactc tacatcagtt
     9841 actgattgtt atggctgtgt ggcgcaagac atacggttac aacaaaaaaa tagattggat
     9901 cggaaacgaa cagttcgctg aactcactgg catggcacca accaaatgct ctaccgccaa
     9961 aaacgagctt atcagaatgg gagttctcat tcaggtgggg cgtcaagttg gtatgaatac
    10021 aaacatttcc gagtggaaaa cgaaagttaa cggattcggt aaaacattta ccaattcggt
    10081 aaaacaaacc ttcaccaaat cggtaaaaag caatttaccg aatcagtcaa acacaaaaga
    10141 caatatacaa aagacaataa atacaaatac ccccttaccc cctaacgggg gcggcgatgg
    10201 gcaggttaaa cctgaacgtc gcaaggcaga acgaatcgac tacgaatcct tcctgaacgc
    10261 ctacaacacc gaagtcggtg acagactgcc acacgctgtt gcggtcaacg agaaacgcaa
    10321 acgccgcctg aagaaaatca tcccgcaact gaaaacgcca aacgtggacg gtttcagagc
    10381 gtatgtcagg gcgtttgtgc atcaggccaa gccgttttac ttcggagaca acgacacggg
    10441 ctggacggcc gattttgatt acctgctgag ggaagactcg ttaacggggg tacgggaagg
    10501 gaagtttgca gacaggggga ttgcatgaaa caggatatcg aagcgagcgt tatcggtggc
    10561 ctgctgattg gtggattaac accaaccgcc agcgacgttc tggcaacgct ggagccggaa
    10621 gcgttttcaa ttccgctcta ccggaaagcc ttcgaggtta tccgcaagca ggcgcgaaac
    10681 agaaacctaa tcgacgcgct gatggttgcc gaggcgtgcg gagaggagca tttcacgtca
    10741 atcctgatga ccagcaagaa ctgcccgagt gccgcaaacc tgaagggata tgctggaatg
    10801 gtcgcggata actatcaccg ccgtctggtg ctggaaatca tggatgaaat gcgtgaacca
    10861 atccaaagcg gaaccatcga cgcatcgagt caggcgatgg atgaacttgt aaagcgtctt
    10921 tcagccatca gaaagccccg tgacgaggta aaacctgtac ggttagggga aatcatcact
    10981 gactacactg acacgcttga caggcgtctg aggaacggag aagagtcaga taccctgaag
    11041 accggaatcg acgaacttga cgccatcacc ggagggatga acgcagaaga cctggtgatt
    11101 atcgccgctc gtcctggtat gggtaaaacc gaactggcgc tgaagattgc cgaaggcgtt
    11161 gcaagccgcg ttattcctgg ttctgacgtc cggcgcggag tgttgatttt ctcgatggaa
    11221 atgagcgcat tgcagattgc agagcggagc attgccaacg ccgggaggat gtcggttagc
    11281 gtgctgcgaa atcctgcatc gatggatgac gaaggctggg cgcgtgttgc taacggcatg
    11341 agtcagcttg cagatttgga tgtatgggta gtcgatgcct cgcggttatc ggtcgaagaa
    11401 atacgctcaa tcgcagaacg gcacaaacag gaaaatccaa acctgtcact catcatggcg
    11461 gattatcttg gcctgattga gaagccgaaa gcagaccgca acgacctcgc aattgctcac
    11521 atctccggaa gcctgaaggc gatggcgaaa gacctgaaaa cgccagttat ctccctgagt
    11581 cagctttcgc gcgatgttga gaagcgacca aacaaacgcc cgacaaacgc agatttgcgt
    11641 gattcaggaa gcattgaaca ggacgcagac tcaatcatca tgctctatcg ggaagcggta
    11701 tatgacgaga acagtagcgc cgcgccattt gctgaaatca tcgtgacgaa aaaccgtttt
    11761 ggctcgcttg gtacggttta ccagcggttc tgtaacggac actttgttgc atgtgaccag
    11821 gatgaagcca gacagatttg cacagcatca aatgcacctg ctgcgcgtgg cagacgatat
    11881 gcacaagggg ctgacgtatg accatctaca tcactgagat aataacaggc ctgctggtaa
    11941 tcgcaggcct ttttatttgg gggagaggga agtgtggctg actggcaaat tccaatcatc
    12001 attcttgccg gagcttcgct ggttgctggc tttatcctgc tgaaaaagca taaagaccgt
    12061 gatcaaaaag ttgaagttct ctatgggtat ccagcgaaca gcacaacatg gctgaccatt
    12121 taccactacc gaaaatcagg acgctgggta ttcgaatggg atgatctgtt cgctgaaaag
    12181 cgaccaaagt catggggaga catcagcgaa tgcatgatgt ttgaagaaag aaaatccggc
    12241 gcaacccgag aagagtttaa cgaagcgtgg gcgcgattaa gtgagagagg gtatttgtga
    12301 gcaagtacga aaaattagat caaaacattc tttcaatgct gagtgaaaga ccaacacctg
    12361 tttttgatat ctggcttaaa tggcggagca atggaatgta tatcgaaacc atcgatcgcc
    12421 gtatgcaata cctgagaaag aaagggcttg ttgcaaatgt gcgtgggaag ggttgggtga
    12481 aaattaacct gtcataacgg ggattgatat ggacgatagc ttagaaatat ttgaagagtg
    12541 gtgcgctgat gaacttggtg tctccgctta cttcattcga caaatgcgaa gcaagaatat
    12601 tttgggcgtg attgagtata aacgagttga aataaacaag agatacaggg cttggatggc
    12661 cgcagttcgc gccgccggag tcaaagtgaa ggagtgagta tgagcgcata cgaagaaatc
    12721 atgttagccc tgcgattctt tttcggtgtg gaagaagatg aaaacgtaaa tgagattatc
    12781 ggacaagacc atgacccgat agggactatt gcagctgcac ttgacgatta caggagcgta
    12841 aatggtgagg aaactaacgt ttgaactaag aagccccatc catcagcaga acgccattca
    12901 agctatccag caaatccttc cagacccaac caaaccaatc gtagtaacca ttcaggaacg
    12961 caaccgcagc ttagaccaga atcgaaagct ttgggcttgc cttggtgacg tttcgcgtca
    13021 ggttgaatgg catggtcgct ggctggatgc agaaagctgg aagtgtgtgt ttaccgcagc
    13081 attaaagcag caggacgttg ttcctaacct tgccgggaat ggatttgtgg taataggccg
    13141 gtcaaccagc aggatgcgtg taggcgaatt tgcggagcta ttagagctta tacaggcatt
    13201 cggtacagaa cgtggcgtta aatggtcaga cgaagcgcga ctggctctgg agtggaaagc
    13261 gagatgggga gataaaactg aatgaacaaa taccgactta tttacgcaga tccgccttgg
    13321 caatatcgcg acaaagccaa cgatggcaat cgcggtgctg gacataaata cgatgttatg
    13381 aatgttcagg acatttgccg actgccagta tgggatttag cggatccaga atcttgcttg
    13441 ttagcgatgt ggtgggtgcc gacacagcca gccgaagcgc taaaggtaat tgaggcgtgg
    13501 ggattcaggt tgatgactat gaaaggcttt acttggcaca aaaccaataa gcacaaaggc
    13561 aacagtgcga tcggaatggg gcatatgacc agggcaaata gcgaggattg cttgtttgcc
    13621 gttcgagggc ggttacctga gagaatggac gcttcaatat gccagcactt taccgcaccg
    13681 agaatggagc acagtgcaaa accaccgatc gtaagagaca tgttagctaa gttgcttggc
    13741 gacgtgccgc gctgtgagtt atttagccgc gacaaagtga ccgggtggga tatgtgggga
    13801 aatcagtgcg actccgattt tgaacttgct cccggcatgg cgattaaacc atgcaaaatg
    13861 gtgatcgcat gaagcactgt tatcgatgtg gagagcgaaa ggaagacgat cgctttcgac
    13921 caggacagcc ttactggaat cgatggtgtc tccggtgtga aagaacacca acaggggtgt
    13981 taccactacc gcaggaaaag gaggacgtgt ggcgagacag cgacgaagta tcaccgacat
    14041 aatctgtgaa actgcaaata ccttccaacg aaacgctcca gaaataaacg caagccaatc
    14101 ccaaaagaat ctgacgtaaa aaccttcaac tacacggctc acctgtggga tatccggtgg
    14161 ctaagacatc gtgcgaggaa atgacaatgc ttttaattca acctggattt ggacttagca
    14221 tcaaaaaagg ccacatgttt ggcgagaaag agtcacaacg aaaaatgatg tctatccggt
    14281 tgccatttat cagtatttat tggctaaaca gggaggcaac aaattattgg tatacatgcg
    14341 ccagagcagc atttaacgac cctgactggt ttgtggaaaa ccaccacgca gttcgtcagg
    14401 caaagagaaa ggccaacatg acatacatga aggcgtatcg aaaagcatgg aaagaacacc
    14461 gcgatcgata ccagcaagat atggaaaagc ttgaatcaga aaacatggaa ttaagacgaa
    14521 agcttggtga agcaaaacga gacattgatg cttacaagcg actttttaat ggtgaaagcc
    14581 atgcttagtc catcccaatc tcttcaatac cagaaagaaa gcgtcgagcg ggctttaacg
    14641 tgcgctaact gcggtcagaa gctgcatgtg ctggaagttc acgtgtgtga gcactgctgc
    14701 gcagaactga tgagcgatcc gaatagttca atgtacgagg aagaagacga tggccaaacc
    14761 agcgcgaaga cgatgtaaaa acgaagaatg tcgggaatgg tttcaccctg cattcgctaa
    14821 tcagtggtgg tgctctccag agtgtggaac caagatagca ctcgaacgac gaagtaaaga
    14881 acgcgaaaaa gcggaaaaag cagcagagaa gaaacgacga cgagaggagc agaaacagaa
    14941 agataaactt aagattcgaa aactcgcctt aaagccccgc agttactgga ttaaacaagc
    15001 ccaacaagcc gtaaacgcct tcatcagaga aagagaccgc gacttaccat gtatctcgtg
    15061 cggaacgctc acgtctgctc agtgggatgc cggacattac cggacaactg ctgcggcacc
    15121 tcaactccga tttgatgaac gcaatattca caagcaatgc gtggtgtgca accagcacaa
    15181 aagcggaaat ctcgttccgt atcgcgtcga actgattaac cgcatcgggc aggaagcagt
    15241 agacgaaatc gaatcaaacc ataaccgcca tcgctggact gtcgtagagt gcaaggcgat
    15301 caaggcagag taccaacaga aactcaaaga cctgcgaaat agcagaagtg aggccgcatg
    15361 acgttctcag taaaaaccat tccagacatg ctcgttgaag catacggaaa ccagacagaa
    15421 gtagcacgca gactgaaatg tagtcgcggt acggtcagaa aatacgttga tgataaagac
    15481 gggaaaatgc acgccatcgt caacgacgtt ctcatggttc atcgcggatg gagtgaaaga
    15541 gatgcgctat tacgaaaaaa ttgatggcag caaataccga aatatttggg tagttggcga
    15601 tctgcacgga tgctacacga acctgatgaa aaaactggag acgataggat tcgacaccaa
    15661 aaaagacctg cttatctcgg ttggcgattt ggtcgatcgc ggtacagaga acgtcgaatg
    15721 cctggaatta atcacattcc cctggttcag agctgtacgt ggaaaccatg agcaaatgat
    15781 gattgatggc ttatcagagc gtggaaacgt caatcactgg ctacttaatg gcggcggctg
    15841 gttctttaat atcgattacg acaaagaaat tctggctaaa gctctttccc ataaagcaga
    15901 tgaacttccg ttaatcatcg aactggtgag caaagataaa aaatatgtca tctgccacgc
    15961 cgattatcct tgtgacgaat acgagtttgg aaagccagtt gatcatcagc aggtaatctg
    16021 gaaccgcgaa cgaatcagca actcacaaga cgggatcgtg aaagaaatca aaggcgcgga
    16081 cacgttcatc tttggtcata cgccagcagt gaaaccactc aaatttgcca accagatgta
    16141 tatcgatacc ggcgcagtgt tctgcggaaa cctcacattg attcaggtac agggagaagg
    16201 cgcatgagac tcgaaagcgt agctaaattt cattcgccaa aaagcccgat gatgagtgac
    16261 tcaccacggg ccacggcttc tgactctctt tccggtactg atgtgatggc tgctatgggg
    16321 atggcgcaat cacaagccgg attcggaatg gctgcattct gcggtaagca tgaactcagc
    16381 cagaacgaca aacaaaaggc tatcaactat ctgatgcaat ttgcacacaa ggtatcgggg
    16441 aaataccgtg gtgtggcaaa gctcgaagga aatactaagg caaaggtact gcaagtgctc
    16501 gcaacattcg cttatgcgga ttattgccgt agtgccgcga cgcctggcgc aagatgcaga
    16561 gattgccacg gtacaggtcg tgcggttgat atagccaaaa cagagcagtg ggggagagtt
    16621 gtcgagaaag agtgcggaag atgcaaaggc gtcggctatt caaggatgcc agcaagcgca
    16681 gcatatcgcg ctgtaacgat gctaatccca aaccttaccc aacccacctg gtcacgcact
    16741 gttaagccgc tgtatgacgc tctggtggtg caatgccaca aggaagagtc aatcgcagac
    16801 aacattttga atgcggtcac acgttagcag catgattgcc acggatggca acatattaac
    16861 ggcatgatat tgacttattg aataaaattg ggtaaatttg actcaacgat ggataaatgc
    16921 actcgttaaa taaagccctg agttaatagc tcggggcttt ttgcgtttta agcacggcct
    16981 ttctgaaagc acatcaagcc aaataccaga cagacaaaaa taatcacctt atccgcttgg
    17041 ctacggtgcg gtgtgctttg cataaaagaa aaccagcgca atggctggct tcgtgaaagc
    17101 gggtggcaag aggctgcgct aacaacctca tgccgttttg cccgtgcata tcggtcacga
    17161 acaaatctga ttactaaaca cagtagcctg gatttgttct atcagtaatc gaccttattc
    17221 ctaattaaat agagcaaatc cccttattgg gggtaagaca tgaagatgcc agaaaaacat
    17281 gacctgttag ccgccattct cgcggcaaag gaacaaggca tcggggcaat ccttgcgttt
    17341 gcaatggcgt accttcgcgg cagatataat ggcggtgcgt ttacaaaaac agtaatcgac
    17401 gcaacgatgt gcgccattat cgcctggttc attcgtgacc ttctcgactt cgccggacta
    17461 agtagcaatc tcgcttatat aacgagcgtg ttcatcggct acatcggtac tgactcgatt
    17521 ggttcgctta tcaaacgctt cgctgctaaa aaagccggag tagaagatgg tggaaatcaa
    17581 taatcaacgt aaggcgttcc tcgatatgct ggcgtggtca gagggaacag ataacggacg
    17641 acagaaaacc agaaatcatg gttatgacgt cattgtaggc ggagagctat ttactgatta
    17701 ctccgatcac cctcgcaaac ttgtcacgct aaacccaaaa ctcaaatcaa cagcagccgg
    17761 gcgctatcag cttctttccc gttggtggga tgcctaccgt aagcagcttg gcctgaaaga
    17821 cttctctccg aaaagccagg acgctgtggc attgcaacag attaaagagc gtggcgcttt
    17881 gccgatgatt gatcgcggtg atatccgtca ggcaatcgac cgttgcagca atatctgggc
    17941 ttcgttgccc ggggctggtt atggccagtt cgagcataag gctgacagcc tgattgcaaa
    18001 attcaaagaa gcaggcggaa cggtcagaga gattgaggta tgagcagagt aaccgcgatt
    18061 atctccgctc tggttatctg catcatcgtc tgcctgtcat gggctgttaa tcattaccgt
    18121 gataacgcca tcgcctacaa agagcagcgc gacaaagccg catccactat cgctgatatg
    18181 cagaagcgtc aacgtgacgt agcagaactc gacgccagat atacaaagga gcttgctgat
    18241 gctaacgcga ctatcgaaag cctccgtgct gatgtttctg ctggtcgtaa gcgcctgcaa
    18301 gtcgccgcca cctgtgcaaa gtcaacgacc ggagccagca gcatgggcga tggagaaagc
    18361 ccaagactta cagcagatgc tgaactcaat tattaccgtc tccgaagcgg aatcgacagg
    18421 ataaccgcgc aggttaacta cctgcaggaa tacatcagga ctcagtgcct gaaataattt
    18481 ttttgcaaat cacaaagtcc atttaatgag cctcgcgatg cggggctttt ttacatctga
    18541 atttcacagc gcatctcacg cgcatattac atcacccgag cctttcagaa agttgagcct
    18601 gagaactgcc gtatatggtg gcgaccatct cgggacggct tttctgtgag acaggctcac
    18661 tttctaaaag gtaaagacgc aatgaactac ccaaccgttg ttaacgatat agatttcaga
    18721 gacctaattt ttgtagcaaa caacgatccg gttacagatt cttttatggt ggcaaaagca
    18781 tttggaaagc tgccgaagaa cgtagttcgt gacattgagc gaaccataga agcttgccct
    18841 cctgagtttg atacaaagct caactttgag ctttgctata aaaacaatga gttacagaat
    18901 ggtaagccgc aaaaattcta ccgtctccgc aaggatgggt tgatgctttt ggttatgtcc
    18961 gacaccaaaa aagaagcaat gcgtatcaaa attgcttaca tcaacgcatt taactggatg
    19021 tacgccatgc ttcaggttgg tcatcgtcaa tttgaagaag agagaaatgc cgtaatgctg
    19081 gagtacatga aagagaagga tgttgccagc atgtcaggtc gcctgctaaa tcgctggggc
    19141 aaaattaaga agccacagct gatggctaga attgaacgcc ttgaacagca cgggcaaacc
    19201 gtaatccccg gactcatcaa ttaacggcag caccgcgaaa caacccaagc cagtaagtgg
    19261 ggaaataaca ctggcagcca ctgaaagatg aacctccagc cttatggcaa aaaagattct
    19321 ttgtggtggc ggactgatgg aaagacatcg gatagaatca agcagtggct agggtagctc
    19381 ccgaaaagcg gactcgtcac cgcctgccac tgaatctatg gcgaacaact agacgaggtt
    19441 gtgatggatg ataacggaaa ccaatgggtc aatgttgaac accggctacc agaaagcaaa
    19501 gaagggatgt ggtctaaaga ggttatagct cttactgata ctggtgacgt gttcaaacta
    19561 tcatgtatgg gctcttactg gcagagaacc aaggcattca tcgattcagg agcaagcaag
    19621 gttacacact ggatgccgct taattaccca gatgattaaa acggatgaaa aatgaaaggt
    19681 cgctcaggcg gcctttttta ttgccatcac aaaagccatt ccttactgag tggctttgat
    19741 aatggcttat accctacacg ggataactta actgatatcc cttttaacgg ataaatggag
    19801 ccaacaatgg cagagattat tcccatgact gaagaacaga aattccagtt agagatttac
    19861 aaactggtca tgaaccagaa cgcagccgca gaagaagcat ttcaattcat tggcactgac
    19921 gaactgaagc ttgagctatt caaaattcac ttccagtcag gtggcgctaa ttcagatatc
    19981 acgatccgca catttgaagc ggtgcgtaaa tcgaaggaag cgttagacct gttcaccacc
    20041 ggagcatgat gctcaacctg aaataacgat taagtgagat gaatatggca gcaccaaagg
    20101 gcaaccgatt ctgggaggcc cgcagtagtc atgggcgaaa ccctaaattc gaatcgcctg
    20161 aggcgctgtg ggctgcttgt tgtgaatact tcgagtgggc tgatgataac ccgctatggg
    20221 agggtaaggt attttcatat cagggagaaa taattaaggc taatgtccct aagatgcgag
    20281 ccatgactat ttcaggattg tgtaccttcc ttgatatcac caggcaaaca tggggaacct
    20341 tccggtcaat ggaaggtttt tctgacgtca catcacgagc ggaagacatc atctacgacc
    20401 agaaattctc tggcgcagcc gctgaccttc tcaacgctaa catcatcgcc cgtgatttgg
    20461 gcctcaaaga gcagtcgcaa gttgaagacg tgacacctga taagggagat cgcgataagc
    20521 ggcgctctcg tatcaaggag ctattcaacc gtggaactgg acgcgattct tgataacttg
    20581 agcgacgaag agcaaatcga attgctcgag ctactcgaag aagaagagaa ctaccgtaac
    20641 acacacctgc tatatgaatt tacgccatac agcaaacagc gtgagttcat cgacgccggg
    20701 catgactatc cagagcgctg ttttatggct ggtaaccagc ttggtaagtc atttactggt
    20761 gctgctgaag tcgcgtttca ccttaccggg cgttatccgg gcacaaaagg ctatcctgct
    20821 gatggtaaat atggcggtga gtggaaaggt aagcgttttt atgagcctgt tgtcttttgg
    20881 attggtggcg agacaaacga gactgtaacc aaaacgactc aacgcatcct gtgtggtcgt
    20941 atcgaagaga atggtgagcc aggctacggt tccataccta aagaagacat cattagctgg
    21001 aagaagtctc ctttctttcc gaaccttgtt gatcaccttc tggttaagca tcacacggct
    21061 gatggcgttg aagatggcat ttcaatctgc tacttcaagc catactcgca aggtcgtgct
    21121 cgctggcagg gtgacacaat ccacggcgtg tggtttgacg aagagccacc atacagcatt
    21181 tatggtgaag gccttacccg taccaacaaa tacgggcaat tctcaattct gacgtttacc
    21241 ccgctgatgg ggatgtctga cgttgttacc aagttcctga agaatccaag caagtcgcag
    21301 aaagtggtca acatgaccat ctacgacgct gagcactaca cagacgaaca gaaagagcaa
    21361 atcatcgcat cttatcctga gcatgagaga gaggcgcgtg ctcgcggtat tcctacgatg
    21421 ggtagtggtc gaatcttcca gataccggaa gagacgatta agtgtcagcc gttcgagtgt
    21481 cctgatcact tctacgtaat tggcgggatg gatttcggat gggatcaccc gcaggcgcag
    21541 gttcagcttt ggtgggacaa ggacgcagac acaatatacg tttcacgcgt gtggaaggcg
    21601 aaagaaaaaa cagccgttca ggcgtgggga gccgttaaat catgggcgca taaagtgcca
    21661 actgcatggc ctcatgacgg aaaccagcac gagaagggcg gcggtgagca gctcaaaggg
    21721 cagtatgcag atgctggttt tatgatgttg caggagcatg cgacatggcc tgatggcggt
    21781 aatgctgtgg agcctggcat cactgaattg cgcgacatga tgctcgatgg tcgcttcaaa
    21841 gtattcaaca cctgtgagcc attctttgag gagttccgcc tctatcaccg tgatgaaaac
    21901 gggaaaatcg tcaagcttaa cgacgacgtt ctctcagccg ttcgctatgc atacatgatg
    21961 cgccgcttcg ccaaaatgat gcgcgacatc aaaaaaccaa aagagaaaaa gataccagcc
    22021 ccaatcaggc ccatcgcacg gagaacttaa atggccgacg aaaacagact caattccatt
    22081 ctgtgtaagt ttgacgcgga ctggatggcg agcgatgaag ccagaaccga ggcgacaaat
    22141 gacctgtatt ttagccgagt gtcgcaatgg gatgactggc tatcaaacta cactaccctg
    22201 caatatcgcg gacaattcga tgttgttcgc ccggaggtca ggaaactggt cgcagagatg
    22261 cgccggaacc ctgtcgacgt tctcttccga cccaaagacg gcgctaatcc tgatgcagcc
    22321 gatgtattga tggggatgta tcgtactgat atgcgccata acacggcaaa gattgccgtt
    22381 aacgttggcg ttcgtgagca gatagagtca ggcgttggtg catggcgtct ggtcacgcag
    22441 tacgaagaca acgatccaac aagcaacaat caggtaatcc gacgcctgcc aatccatgaa
    22501 gcctgctcac acgtcatatg ggacgccaac agcaagcaga tggataagag cgacgctaag
    22561 cactgcacgg tgattaacgc cttgtcgcgc aatggctgga aagagttcgc agaggattac
    22621 ggtattgacc cggataccct gccatctttc cagaatccta acgatacatg gctattcccg
    22681 tgggtgtcga atgatgtcgt ctacgtcgct gagtattacg aggtagaaga ggagaaggag
    22741 aaggtcttca tctaccgcga cccgctgaca ggtgagccgg tcagctatta ccagcaggat
    22801 atcaaagacg tcatcgacga cctggctaat cgtggattca ttaaggtagc agagcgcaag
    22861 gtgaagcgtc ggcgtgtgta taagtcgatc atcacctgta cgcagatact gaaagaccgc
    22921 gagaagatag ccggagagca tattccaatc gttccagtgt atggcgaatg g
//
LOCUS       DAZPAB010000046        22855 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZPAB010000046 DAZPAB010000000
VERSION     DAZPAB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22855
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.046"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..229)
                     /locus_tag="ACHUPG_001628"
     CDS             complement(<1..229)
                     /locus_tag="ACHUPG_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase YliE domain
                     protein"
                     /protein_id="HFR0861291.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIRLIILFLLAA
                     LSFIGLFFMINYQLVSERAVKRADSRFELIQK"
     gene            complement(329..1240)
                     /gene="gsiD"
                     /locus_tag="ACHUPG_001629"
     CDS             complement(329..1240)
                     /gene="gsiD"
                     /locus_tag="ACHUPG_001629"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR0861292.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1243..2163)
                     /gene="gsiC"
                     /locus_tag="ACHUPG_001630"
     CDS             complement(1243..2163)
                     /gene="gsiC"
                     /locus_tag="ACHUPG_001630"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR0861293.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2181..3719)
                     /gene="gsiB"
                     /locus_tag="ACHUPG_001631"
     CDS             complement(2181..3719)
                     /gene="gsiB"
                     /locus_tag="ACHUPG_001631"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR0861294.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3739..5610)
                     /gene="gsiA"
                     /locus_tag="ACHUPG_001632"
     CDS             complement(3739..5610)
                     /gene="gsiA"
                     /locus_tag="ACHUPG_001632"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR0861295.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5597..6562)
                     /gene="iaaA"
                     /locus_tag="ACHUPG_001633"
     CDS             complement(5597..6562)
                     /gene="iaaA"
                     /locus_tag="ACHUPG_001633"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR0861296.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6766..8001
                     /gene="moeA"
                     /locus_tag="ACHUPG_001634"
     CDS             6766..8001
                     /gene="moeA"
                     /locus_tag="ACHUPG_001634"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR0861297.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            8001..8750
                     /gene="moeB"
                     /locus_tag="ACHUPG_001635"
     CDS             8001..8750
                     /gene="moeB"
                     /locus_tag="ACHUPG_001635"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR0861298.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8929..9591)
                     /gene="fsa"
                     /locus_tag="ACHUPG_001636"
     CDS             complement(8929..9591)
                     /gene="fsa"
                     /locus_tag="ACHUPG_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR0861299.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            9722..10621
                     /locus_tag="ACHUPG_001637"
     CDS             9722..10621
                     /locus_tag="ACHUPG_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR0861300.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10627..13059
                     /locus_tag="ACHUPG_001638"
     CDS             10627..13059
                     /locus_tag="ACHUPG_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR0861301.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13205..14020
                     /gene="ybiV"
                     /locus_tag="ACHUPG_001639"
     CDS             13205..14020
                     /gene="ybiV"
                     /locus_tag="ACHUPG_001639"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR0861302.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14172..15437
                     /locus_tag="ACHUPG_001640"
     CDS             14172..15437
                     /locus_tag="ACHUPG_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR0861303.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(15678..17270)
                     /locus_tag="ACHUPG_001641"
     CDS             complement(15678..17270)
                     /locus_tag="ACHUPG_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR0861304.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            17489..18409
                     /gene="ldtB"
                     /locus_tag="ACHUPG_001642"
     CDS             17489..18409
                     /gene="ldtB"
                     /locus_tag="ACHUPG_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR0861305.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18467..19585)
                     /locus_tag="ACHUPG_001643"
     CDS             complement(18467..19585)
                     /locus_tag="ACHUPG_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR0861306.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(19582..20049)
                     /gene="mntR"
                     /locus_tag="ACHUPG_001644"
     CDS             complement(19582..20049)
                     /gene="mntR"
                     /locus_tag="ACHUPG_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR0861307.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20235..20363
                     /gene="mntS"
                     /locus_tag="ACHUPG_001645"
     CDS             20235..20363
                     /gene="mntS"
                     /locus_tag="ACHUPG_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR0861308.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            20635..22218
                     /gene="opgE"
                     /locus_tag="ACHUPG_001646"
     CDS             20635..22218
                     /gene="opgE"
                     /locus_tag="ACHUPG_001646"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR0861309.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22266..22781)
                     /gene="ompX"
                     /locus_tag="ACHUPG_001647"
     CDS             complement(22266..22781)
                     /gene="ompX"
                     /locus_tag="ACHUPG_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR0861310.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
BASE COUNT         5620 a         6256 c         5687 g         5292 t
ORIGIN      
        1 ttttctgaat aagttcaaag cggctatcgg cacgttttac cgcgcgttcc gataccagtt
       61 gatagttaat catgaaaaaa agaccaataa atgacagtgc tgccaataaa aataaaatta
      121 tcagccttat cttttcgtat aaactcagca tgttaataca tttaacaaaa taactatctg
      181 aacgctgtgc cgctgcaaaa atagtccctt gctgcggctt tttaaccaaa ttacccgatt
      241 ctacaaaatc agcagaaagt tacaacactt gcttagggca ataatttcct gtgaggtgat
      301 taccctttca agcaatattc aaacgtaact atcctttaat tttcggatcc agcgcatcgc
      361 gtaaaccatc gcccaacaaa ttgaacgcca gtacggtcag aaaaatagcc agggccggaa
      421 aaacagcgac atgcggcgcg ataaccatat ccgctcgtgc ctcattgagc attgctcccc
      481 actctggtgt cggcggctgc gcaccgaggc cgagaaatga caggctggcg gcagagataa
      541 tcgaggtgcc aatgcgcatg gtgaaaaaca ccacgataga agagacggtc ccaggcagga
      601 tatgacgcaa taaaatggtc atatcgctgg caccaatact gcgtgctgac tcaataaagg
      661 tttgctgttt caacaccagc gtgttgccgc gcaccaggcg ggcaaacgcg gggatggaaa
      721 aaatggcgac tgcgataatc acgttagcaa tgccgcttcc caacaccgca acaacagcga
      781 tcgccagtaa aatacccggg aaggcaaaca gcacatcgca aatgcgcatg atcagccgat
      841 cccaccagcc ttcataatat ccagccaaca agcccagcaa cgtccctatc gccacaccga
      901 taaacacggc aaacacgccc gccgccagcg agatttgcgc accaaccagg acacggctga
      961 aaatgtcgcg ccccagtgaa tcgacgccaa accagtgctg caaagaaggt ccgttattca
     1021 gattgtcata atcaaaataa ttttcggcgt catagggagc gatccagcgt gcaaaaatgg
     1081 ccaccacaat caataaaata acgaataatg cggcggtcat cgccatatgc tgacggcgaa
     1141 atcgtcgcca gaattcatgc cacggtgtac gtacctggtc aggtttgacc agtggcatgg
     1201 cgtttaacac cgcctgacgt cgccagttaa atagtcgcat ccttacttgt acctgatagc
     1261 cgggttaatg gcggcgtaaa gcacatccac cactaagttg ataagaataa attccagcga
     1321 gaaaagcaga atttccgcct gaatcaccgg gtaatcgcgc atttctacag agtcaacgag
     1381 taagcgtcca agtccgggcc agttgaaaac tttctccacc acgatagaac caccgagcaa
     1441 aaagccaaac tgtaagccca tcatggtcac taccgggatc atcgcgttgc gtagcccgtg
     1501 tttgaggaca acccaggttt cgctcacccc tttcgccctc gcggtacgca tataatcttc
     1561 gcttaaaacg tcgacaaatg acgcacgggt aaagcgcgcc atcacggcgg ccactgccgc
     1621 gccgagcgtc agggagggta aaatgtagtg ctgccaactg tctgctccca cagtaggcag
     1681 ccagcccagt tcaacggaga atacctgaat taaaaacatc cccagcgcaa atgccggaaa
     1741 cgagatcccc gacaccgcaa tggtcatact caatcgatcc ggccaacgat tacgccagac
     1801 ggcggcgata attcccgccg ccataccaaa tatcaccgcc cagaccatac tggttatggt
     1861 cagccatagc gttggcataa agcggctggc aatctcatcg gcaaccggac gacgcgacac
     1921 catcgacagg ccaaaatccc cctgcacagc attgctgata tagtgccaga actggtgata
     1981 cagcggctga tccaacccca gctgctgacg caccagttct ataacctgcg catcagcttc
     2041 gggcccggca atcaatcgcg ccggatcgcc gggcagcatg tggacaaata aaaacaccag
     2101 caccgagacg ataaacagcg tcggaatcaa ccccagtaat cgtttgataa cgtaataaag
     2161 cattccactc cctgcgttgc ttattgcaaa tccgcgtctt caaagctgaa gccggtatct
     2221 ggcatgatcc aaaaaccggt caggttttta ctgtgtgccg acaccagttt ttctaccacc
     2281 agcgggatcc acggcgattc ttgccagatg atatcctgcg ccgccttata taagcgggtc
     2341 ttttccgccg gatcattcgt tttcagtgcc tgagccagga agtcatccac ctgtttattg
     2401 ctgtaaaacg cggtattaaa cagcgtcggt ggccagtttt gcgaggcaaa cagcggcgat
     2461 agtgcccagt cggcttcgcc ggttgaagcc gaccagccag tgtagaacat ccgcacgcca
     2521 ctctcttttt gccctttacc ttcaacttct gccgcccgct gtccggcatc catcgcggtc
     2581 acctgggctt taatcccgac ctgcgctaac tgctgctggg taaattgcag cactttctgc
     2641 gcggtgctgt ggttatgtga cgaccacagc gtggtactga aaccgttggg atatcccgcc
     2701 tctttcagta attcgcgcgc tttcactgga tcgtaaggcc acggtttata actttgcgcg
     2761 taggcgatac ttggcggtac cacaccagta gctggagttg catagcccgc aaaggcaact
     2821 ttcaccagcg ccggacggtt aatggcgtaa ttcagcgcct cacggacctt cgggttatcg
     2881 aacggctttt gcgtcacgtt catactgata taacgctgca taattgacgg actggccatc
     2941 aactcgatat ttttgttttt ctccagcagt gtggcctgct cgtagggaat ggggaaagca
     3001 aactgcgctt caccggtttg cagcattgcc gcgcgggtgt tgttatccgc caccggacgc
     3061 caggttatgc tgtccagttt gggcaatcct ggctgccagt aacccgcgaa ttttttcacc
     3121 ttcacaaaat cggtctgatt ccaggtatcc agttcatacg gtccggttcc caccggatga
     3181 aaaccaatct ccttgccata tttttccagc gctgtcggtg aaatcatcgc ggtcgccgga
     3241 tgggcaagaa tattaataaa cgctgagaac ggctgtttga gggtaatctt taccgttgtc
     3301 ggatcgatcg cctccgtttt ggcaatattc ttatacaggt tatagcgttt aagatgattc
     3361 gccggatcgc tggcccggtc cagattcgct tttaccgcca ctgcgttgaa atcggtgcca
     3421 tcctggaatt taattccttc ccgcaatttc acggtgtaag taatgccgtc atcggaaacg
     3481 gtataactct ccgccagcac gtttttcagt ttcatctctt tatccagacc gaacagcccc
     3541 tggtaaaacg atttcgctac ggcctgagat aacgtgtcat tcgcgtcata cggatcgagc
     3601 gtggtgaaat tcgatcctac cgccaccacc acatctttgg cagcgaatgc acaagatgcc
     3661 atcaacgctg tcgcaatgcc cagcgccact aacccactac ggtgtacagc tcttgccatt
     3721 ttattctccg cctgaatgtt atctacgcat gaatgcgtat tctgattgtg gttgtgcgac
     3781 gtaatgcccc ggcccgacgc attgcaacga gacggctgcc acctcttcgc cacgcagatg
     3841 aatattgctg ggaagatcgt ccgacagcag tacacgctgc ggtcgttgtc gggacggttc
     3901 agcgaccgga actgccgcca gtaatttacg cgtataagga tgctgcgggt tttcgaagac
     3961 cgcgcaccgt gaaccaattt caacaatttg cccgagatac atcaccgcca cacgatgact
     4021 aatccgctct accacagcca tatcgtggga gataaacaga tacgcaatgc cgaaatcacg
     4081 ctggagatcg agcaacaagt tgataatctg cccgcgaata gaaacatcca gcgccgaaac
     4141 ggcttcgtcg gcaatgatca cttttggatt caatgccaac gcgcgagcaa tgcagatgcg
     4201 ctggcgctga ccgccggaaa actcatgcgg gtaacgccag gcatgttcag gtaacaggcc
     4261 cacgcgctcc agcaaccacg caacgcgtgc agccgcgtct ttacctggca ataaaccgtg
     4321 tacacgcagc ggttcgataa tcgaatcacc gatggtctga cgtgggtcca gcgaagcgta
     4381 agggtcctga aaaataaact gaatatcccg gcgcaatgcc tgaagtttgc caggtgacaa
     4441 ggtatcgatt cgctgaccgt taaagataat ttcgccgccc tgcgattcga ccaggcgcag
     4501 caacgcccgc ccggtagtgg atttaccgct gccagactcg cccaccagcg atagcgtttc
     4561 gccaggccag agatcaaaac tgactttctc aacggcatgc acttcccgcg ttacgcgatt
     4621 caacaaaccg ctgcgcaaag ggaaacgggt gacaagatta cgcacccgta aaacaggttc
     4681 gccatccacc accgttttct gctcgatggg gggttcctgt ttcgctggat gttcaagcga
     4741 tatcaatggg aaacgtcggg gataatctaa ccctttcatc gcaccaagtt gcggaacagc
     4801 agctaacagc gcacgggtgt aaggatgttg cggtgcatga aaaatctgtt cgacggtacc
     4861 cgtttccacc gcctcgccct gatacatcac cagtacccga tcggcaatct ctgccaccac
     4921 gcccatatcg tgagtgataa agataacgcc catcgacatc tctttttgca atactttgat
     4981 taattgcagg atctgcgcct gaatagtgac atccagcgcg gtggttggct catcggcaat
     5041 cagcaccgcc gggcggcatg acagcgccat cgcaatcatc actcgctggc gcatcccgcc
     5101 agagagttga tgcgaataac gtgaaagaat ggtttgtgcc tccggaatgc gtacctgatc
     5161 cagcatccgc ttcgcctcga ccatcgcttc ttcacgactg gcgttctgat gcagacgaat
     5221 tgattcggca atctgttcac ccacagtaaa tactgggttc agcgatgtca tcggctcctg
     5281 aaatatcatc gccatatccg caccgcgcac atggcgcatt tgtgcagcgc tctgctcgct
     5341 aagttcaatc acatcacgac tgcgccgccg caacagcatt ttatcgcact gtactaaacc
     5401 gcccgcctgt tccaacagac gcatcaatgc cagcgcagtc actgacttac cggagccgga
     5461 ttcgcctaca attgccagcg tctcaccgcg ttgcagacta aaagagagat tgcggaccgc
     5521 agctattttc tgctggtcct gcataaaggc aatattaaga ttttcaaccg ccagcacatt
     5581 accggcatca agttcatcac tgtgtggcaa cggtgtcccc tttttcacgg tagataccgg
     5641 tggttggcgt atcgcctgcg tagccccagg cgcgatacat tccttcggtg ttaaacggta
     5701 gcgcgacatt cccttcatgg tcgatagcga ttaagccacc gctaccgcca agcgcaggga
     5761 gtttttccat tactacccgc tcgcaggctt ccgcgagact taatccgccg taatccatta
     5821 acgcggcgat gtcatatgcc gccagcgcgc ggatgaagac ttcgcctgtg ccggtacaag
     5881 aaaccgccac actggcgtta ttggcgtagc atccggcacc cactaaggga ctatcgccaa
     5941 ctcgtccggg taatttattg gtcattccgc ctgtggacgt ggctgccgcc agattgccgt
     6001 ctaaatccaa cgccacggcc cccacggtgc ccattttctg tttttcatcc agtggcgcac
     6061 cgctatggtc gaggactgtt gccccttcct cgcgcgctgc cagtagttgt tcataacgca
     6121 aaggcgtgga gaaaatctcc ggtgagacgc gctccatgcc atgagcaaac gcaaaatttt
     6181 ctgccccttc gccaatcatc atcacatgcg ggctttgctc catcaccagc cgggcggcaa
     6241 gaaccggatt acgcagatga ctaacgcccg ccaccgcacc ggctttcagg gtgttaccat
     6301 ccatcacaca ggcgtccagt tcatgggttt catcacgcgt aaagacagcg ccaattccgg
     6361 cgttaaacag tggacactct tccagcagac gcaccgcttc cgtcaccaca tccagcgcac
     6421 tttcgccggc ttccagcatt ttctgcccgg tttcaacaat ggcagacaac gcctcgatgt
     6481 agcgtaattc ctgttgcaga ctcatctgcg cgcggctaat tgcacctgcg ccaccatgaa
     6541 ttgcaatgac tgctttgccc atagtattcg tccattaagg gtggctcgcc acttttgcta
     6601 taaatatcag aatttttatg aattactcat tcaatttttg aatatagaaa gatggatatg
     6661 gcatgtaaag gcaggagcca acgctacgcc cgaggtggca tacagtctat ggcgtttttc
     6721 tgacataata ggcaaattcg attttgcctc cgcaggagtg ttttcatgga atttaccacc
     6781 ggattgatgt cgctcgacac cgcgcttaat gagatgcttt ctcgcgttac cccactgacc
     6841 acccaggaaa cgctgccact ggttcagtgt tttggtcgta ttctggcgag cgatgtcgtt
     6901 tcgccactgg atgtcccggg gtttgataac tccgctatgg acggctacgc ggtgcgttta
     6961 gccgatattg cctccgggca accgctgccc gttgccggta aatcctttgc cggtcagcca
     7021 taccatggtg aatggcctgc gggtacctgc attcgtatta tgaccggtgc gccggtgccg
     7081 gaaggctgcg aagcggtggt gatgcaggag cagactgaac aaacggacaa tggcgtgcgt
     7141 tttactgctg aagtgcgtag cgggcaaaat attcgccgtc gcggtgaaga tatctctgca
     7201 ggtgcggttg ttttcccggc gggaactcgc ctgactaccg cagagctgcc agtaattgct
     7261 tcactgggga ttgccgaagt tccggtgatt cgtaaagtgc gtgtagcgct tttttctacc
     7321 ggtgatgaac tccagttgcc cggtcagccg ctgggcgacg gccaaatcta cgacaccaac
     7381 cgtctcgccg tacacctgat gttagaacag ctgggttgcg aggtaatcaa cttagggatt
     7441 atccgcgacg atccacatgc cctgcgcgcc gcatttattg aagccgacag ccaggcggat
     7501 gtagtgatca gttccggcgg tgtttcagtg ggtgaagcgg attacaccaa aatgattctt
     7561 gaagagctgg gggagatcgc cttctggaag ctggcgatta aaccaggtaa accgttcgcg
     7621 ttcggtaaac tcagcaatag ctggttctgc ggcctgccgg gcaacccggt ttcagcgacg
     7681 ctgaccttct atcaactggt acagcctttg ctggcaaaac taagcggcaa caccgccagc
     7741 ggcctgcccg cgcgccagcg cgtacgcaca gcgtcccgcc tgaaaaaaac gccaggacgt
     7801 cttgatttcc agcgcggcgt gctgcaacgc aacgccgacg gtgaacttga agtgacgacc
     7861 accggacatc agggttcaca tatatttagc tcctttagtc tcggcaactg ctttatcgtg
     7921 ctggaacgcg atcgcggcaa tgtggacgtg cgcgaatggg tggaagtaga accgtttaac
     7981 gcgttgttcg gaggcctgta atggcggaac tcagcgatca ggagatgctg cgctacaacc
     8041 ggcaaatcat tctgcgcggt tttgattttg acggtcagga ggcgctaaaa gattctcgtg
     8101 tgctggtagt cggcctcggt ggactcggct gcgcagcttc gcagtatctg gcaagcgccg
     8161 gtgtcggcaa cctgacgctg ctcgacttcg acacggtttc gctctcgaat ctgcaacgcc
     8221 agacactgca cagtgatgcc acggtcgggc aaccgaaggt ggaatccgcc cgtgacgccc
     8281 tgacgcggat caacccacat atcgcgatta cgccagtcaa tgcactgcta gatgacgcag
     8341 aacttgcagc attgattgct gaacacgatc tggtgctcga ctgtacggat aacgttgcgg
     8401 tacgtaatca actgaacgca ggctgttttg ccgcgaaggt gccgctggtt tccggcgcgg
     8461 caattcgtat ggaagggcaa atcacggtct tcacttatca ggacggcgaa ccgtgctatc
     8521 gctgccttag ccgtttgttt ggcgagaacg cgttaacctg cgtagaagca ggcgtgatgg
     8581 caccgttgat aggcgtaatt ggttcgttgc aggcaatgga agcgatcaaa ctgctggcag
     8641 gttatggcaa accggccagc gggaaaatcg tcatgtacga tgcgatgacc tgtcagtttc
     8701 gcgaaatgaa actgatgcgc aatccggggt gtgaggtgtg cgggcagtaa ttgccacctc
     8761 caagcctgat aagacgcgac aagcgtcgca tcaggcattt gctccagacg ccagatgcgg
     8821 cgtgaacgcc ttatctggcc tacggttcgg gcacagattt ccaagcgcgg caggcatcgg
     8881 tgcactccgc cacgtcatca gcaatttatg aggatgcaga acgtccggtt aaatcgacgt
     8941 tctgccaaac gctccctgcc agtcctgctc aaacttcgtc acagcggctt ccaccgccgg
     9001 atagctaatc atctgttgtg ccacatccag tggcagagta attgattcac atccggccag
     9061 taagcagtcc agcgcctgac gcggggtttt gaaactcgct gccagcactt tcgcctgcgg
     9121 cgcatgcatt ttcaataact ggtgtaagtc ggtcacagtc tgaatgccgc taccgccctg
     9181 agcatcaata cgattaacgt aaggcgcaac atattccgca cctgccagcg ccgacagcag
     9241 cccttgtgcc gcgccatata ccgcggttcc cagcgtcgga atcccttccg cttttaacat
     9301 cttaatcgct gccagcccct cggctgtcac cggaactttc accacgatat ccgcaataat
     9361 agaacgcagc ttaagcgcgt cattcaccat cccttcagca gtggtagcca ttacctgggc
     9421 aaacagacgc ccctgaccgc ccatcgcttc atgaagttgc ggaagcacaa catccagcgg
     9481 ttttttaccc gcggcgataa tgcttgggtt agtggtcaca cccgccagcg gaaaaatacg
     9541 tgacagcgcc ttcaccgcaa caacgtctga agtatccaga tacagttcca taaccatcct
     9601 caaaatgtac tttttgtctg aatgtagact atcacgggaa aactctctga acagttgacg
     9661 cacatcaatc taactttcat tcgaaagtat tttaatgttt gaacgaaaca gagaggcaac
     9721 tatgattttc aatattcagc gctactcgac ccatgatggc cccggtatcc gcacggtcgt
     9781 atttcttaaa ggctgttcgc tgggctgccg ctggtgtcag aacccggaaa gccgcgcccg
     9841 tacgcaggat ctgctgtatg acgcacgact gtgtctggaa ggctgcgagc tgtgcgctaa
     9901 ggccgcgccg gaagtgattg agcgcgcgct gaatggtttg cttattcatc gggaaaagtt
     9961 aaccccggag catctgacgg cgttaaccga ctgctgtccg acacaggcgt taaccgtgtg
    10021 tggtgaagtg aaaagcgttg aggagatcat ggcgaccgtt ctgcgcgata aaccgtttta
    10081 cgatcgcagc ggcggcggct taacgctttc gggtggtgaa cccttcatgc agccggaaat
    10141 ggcgacggcg ctgctgcaag cctgccacga ggcaggcatt catactgcgg tagaaacctg
    10201 tctgcatgtg ccgtggaaat atatcgcccc ttctctgccc tatatcgatc tgtttcttgc
    10261 cgatttaaaa cacgttgccg acgcgccgtt taaacagtgg accgacggta acgccgccag
    10321 ggtgctggat aacctgaaaa aactcgccgc agcgggcaaa aaaatcatta tccgcgtgcc
    10381 gctgattcag ggctttaatg ccgacgaaac ctctgtaaaa gccattaccg attttgccgc
    10441 cgacgagctg cacgttggcg aaattcattt tctgccctac cacacgctgg gcatcaacaa
    10501 atatcactta cttaatctgc cctatgacgc cccggaaaaa ccgcttgatg cgccagagct
    10561 gctcgatttt gcccagcagt atgcctgcca gaaagggtta accgcgacct tacgaggata
    10621 acaaccatga ccacactgaa actggacacg ctcagcgacc gcattaaagc gcacaaaaat
    10681 gcgctggtgc atattgtgaa gccgccagtc tgtaccgagc gcgcgcagca ctataccgag
    10741 atgtatcaac aacatctcga taagccgatc ccggtacgtc gcgcgctggc actggcgcat
    10801 cacctggcga atcgcaccat ctggatcaaa cacgatgagt tgatcattgg caaccaggca
    10861 agcgaagttc gcgccgcgcc gatcttcccg gaatatactg tctcgtggat cgaaaaagag
    10921 attgatgatc tggcagatcg tcccggtgct ggctttgcgg tgagcgaaga gaacaaacgt
    10981 gttctgcatg aagtgtgccc gtggtggcgc ggtcagacgg tacaggatcg ctgctatggc
    11041 atgtttaccg atgagcaaaa aggtctgctg gcgaccggca tcattaaagc ggaaggcaat
    11101 atgacctccg gcgatgcgca cctggcggtc aatttcccgc tgctgctgga aaaagggctt
    11161 gatggtctgc gtgagaaagt agcggaacgt cgctcgcgca tcaatctgac ggtgctggaa
    11221 gatttgcacg gcgaacagtt cctgaaagcg attgatatca tgctggtggc agtcagtgaa
    11281 cacattgaac gtttcgctgc cctggcgcgt gcaatggccg cgaccgaaac ccgcgaaagc
    11341 cgtcgcgatg aactgctggc gatggcagaa aactgcgatc ttatcgccca ccagccgccg
    11401 cagactttct ggcaggcgct gcaactgtgt tacttcatcc agttgatttt gcagattgaa
    11461 tctaacggtc actcggtgtc gtttggtcgt atggatcagt atctctaccc gtactatcgc
    11521 cgcgacgttg aactgaacca gacgctggat cgcgaacacg ccatcgaatt gctgcatagc
    11581 tgctggttga aattgctgga agtgaacaaa atccgctccg gctcccactc aaaagcctct
    11641 gcgggaagtc cgctgtatca gaacgtcacc attggcggac aaaatctggt tgatggtcaa
    11701 ccaatggacg cggtgaatcc actctcttac gcgatcctcg aatcctgcgg tcgcctgcgt
    11761 tccactcagc ctaacctcag cgtgcgttac catgcaggaa tgagtaacga tttcctcgac
    11821 gcctgcgtac atgtgatccg ctgcggcttc gggatgccgg cattcaacaa cgacgaaatc
    11881 gtgatcccgg aatttattaa actcggtatt gaaccgcagg acgcttatga ctacgcagcg
    11941 attggttgta tagaaaccgc cgtcggtggc aaatggggct atcgctgtac cggcatgagc
    12001 tttatcaact tcgcccgcgt gatgctggcg gcgctggaag gcgggcgtga tgccaccagc
    12061 ggcaaagtgt tcctgccaca agaaaaagcg ttgtcggcag gtaacttcaa caacttcgat
    12121 gaagtgatgg acgcgtggga tacgcaaatc cgttactaca cgcgtaaatc aatcgaaatc
    12181 gaatatgtcg tcgacaccat gctggaagag aacgtgcacg atattctctg ctcggcgctg
    12241 gtggatgact gtattgagcg agcgaaaagt atcaagcaag gcggcgcgaa atatgactgg
    12301 gtttctggcc tgcaggtcgg tattgccaac ctcggcaaca gcctggcggc agtgaagaaa
    12361 ctggtgtttg aacagggcgc gattggtcag caacagctgg ctgccgcact ggcggatgac
    12421 ttcgatggcc tgactcatga gcaactgcgt cagcggctga ttaacggtgc gccgaagtac
    12481 ggcaacgacg atgataccgt cgatacgctg ctggctcgcg cttatcagac ctatatcgac
    12541 gaactgaaac agtaccataa cccacgctac ggtcgtggtc cggttggcgg caactattac
    12601 gcgggtacgt cgtctatctc cgctaacgta ccgtttggcg cgcaaactat ggcaacgccg
    12661 gacgggcgta aagcccacac cccgctggca gaaggcgcta gtccggcctc tggtactgac
    12721 catctcggcc ctactgcggt cattggctca gtgggtaaac tgcctacggc agcgattctc
    12781 ggcggcgtgt tgctcaacca gaaactgaat ccggcaacgc tggagaacga atctgacaag
    12841 cagaaactga tgatcctgct gcgtaccttc tttgaagtgc ataaaggctg gcatattcag
    12901 tacaacatcg tttcccgcga aacgctgctg gaggcgaaaa aacatccgga tcagtaccgc
    12961 gatctggtag tgcgtgtcgc gggttattcc gccttcttca ccgcgctctc tccagacgct
    13021 caggacgata tcatcgcccg tactgaacat atgctgtaat ctcttcggcc cggctgcggc
    13081 gtcgccgggc taaatcattc acatcatcaa tttcatcctt actttcattc gaaatataat
    13141 ttgtgctctg cgtcacattg ttattacact gttacggttt cattttcgag ccaggagcac
    13201 acctatgagc gtaaaagtta tcgtcacaga tatggacggt acttttctta acgacgccaa
    13261 aacgtacaac caaccacgtt ttatggcgca atatcaggaa ctgaaaaagc gcggcattaa
    13321 gttcgttgtc gccagcggta atcagtatta ccagcttatt tcgttctttc ctgagctaaa
    13381 ggatgagatc tcttttgtcg cggaaaacgg cgcactggtt tacgaacatg gcaagcaact
    13441 gttccacggc gaactgaccc gacatgaatc gcggattgtt attggcgagt tgctaaaaga
    13501 taagcaactc aattttgtcg cctgcggtct gcaaagtgca tatgtcagcg aaaatgcccc
    13561 cgaagcattt gtcgcactga tggcaaaaca ctaccatcgc ctgaaacctg taaaagatta
    13621 tcaggagatt gacgacgtac tgttcaagtt ttcgctcaac ctgccggatg aacaaatccc
    13681 gttagtgatc gacaaactac acatagcgct ggatggcatt atgaaacccg tcaccagtgg
    13741 ttttggcttt atcgacctga ttattcccgg tctacataaa gcaaacggta tttcgcggtt
    13801 actgaaacgc tgggatctgt caccgcaaaa tgtggtagcg attggcgaca gcggtaacga
    13861 tgcggagatg ctgaaaatgg cgcgttattc ctttgcgatg ggcaatgctg cggaaaacat
    13921 taaacaaatc gcccgttacg ctaccgatga taataatcat gaaggcgcgc tgaatgtgat
    13981 tcaggctgtg ctggataaca catccccttt taacagctga cctctctacc ggagccatgc
    14041 gctccggttt tcccttcttt ttacatcctg tacatcacat caaatcatta aacttgcatt
    14101 aacttattta taacttgtat tgtcacatgc aaattagcat agtttcgaat gaaagttaat
    14161 gcaaggtggt tatggcttct acttttacca gcgacacatt gcctgccgat cacaaagcag
    14221 ctatccgtca gatgaagcac gcgctgcggg cgcagcttgg cgatgtccag cagatcttta
    14281 atcagctaag cgatgacatt gccacgcgag tggctgaaat caacgcactc aaagcacagg
    14341 gcgatgccgt ctggccggtg ctgtcttatg ccgatatcaa agcaggtcat gttactgcag
    14401 agcagcgcga acagattaaa cgtcgcggtt gtgcggtgat aaaaggccat ttcccccgcg
    14461 aacaagcgct aggctgggat cagtcgatgc tggactatct gaaccgcaac cgctttgacg
    14521 aggtctacaa aggccccggc gataatttct tcgggacgct cagcgcttca cgtcccgaga
    14581 tttaccccat ctactggtcg caggcgcaaa tgcaggcccg ccagagtgaa gaaatggcga
    14641 atgcgcagtc gtttctcaat cgtctgtgga catttgaaag tgatggaaag caatggttta
    14701 acccggatgt gagcgtcatc taccctgacc gtatccgccg ccttccgccc ggaacgacct
    14761 ccaaaggtct tggagcgcat accgactccg gggcgctgga acgctggctg cttccagcgt
    14821 atcagcacgt tttcgccaac gtctttaatg gcaatctggc gaaatacgat ccctggcatg
    14881 cggcacatcg tacggaagtt gaagagtaca cggtggacaa caccaccaaa tgttccgtgt
    14941 ttcggacatt ccagggctgg actgcgctct cggatatgct gcctggtcag gggttgctgc
    15001 acgtcgtgcc cattcctgaa gccatggcgt acgtactgtt acgtccgctg cttgatgatg
    15061 tgccggagga tgaactgtgc ggcgtagcgc ccggaagagt gttgccggta tcagagcaat
    15121 ggcatccact gttaattgag gcgttaacca gcattccaaa actcgaggcc ggagactccg
    15181 tctggtggca ctgcgacgtc atccattccg ttgcccccgt tgaaaatcaa cagggttggg
    15241 gcaacgtgat gtacattcct gcggcaccga tgtgcgagaa aaatcttgcc tacgcgcaca
    15301 aggtgaaggc cgcactggaa aaaggcgcat cgccgggcga cttcccgcgc gaggactatg
    15361 aaacaaactg ggaaggacgc tttacgcttg ccgacctcaa cattcacggt aagcgagcgt
    15421 tgggcatgga tgtttgagag ttaatggaag tgaattgatg tgggattaag aaaatcgatt
    15481 cgacgttact cagattgctg acaagtgcgc tttcttaatg cctgatgcgg cgttaacgcc
    15541 ttatccggcc tacaaagtcc tgcagattca atatattaca ggtactgcgt aggcctgata
    15601 agcgtagcgc atcaggctat tttgcatttg tcatcagccg tcgtcacccc gacagtatcg
    15661 gggtgacgga taatcactta ctcgatccct ttactacgca ggtaatcttc gtaattaccg
    15721 ctaaagtcga tcacgcgttc cggggtgatt tccagaatgc gggtcgccag ggagcttacg
    15781 aactcacggt cgtgtgaaac aaagatcagc gtgccctgat acagttccag cgccatgttc
    15841 agtgactcga tggattccat atccaggtgg ttggtcggtt cgtccatgat cagaatgttc
    15901 ggcttctgca tcattaactt accaaacagc atccgccctt tttccccacc ggaaagcact
    15961 ttagctggct ttttgatgtc gtcctggctg aacagcaaac gaccgagaat gctgcgtacc
    16021 gcctgctcgt catcgccttc ctgcttccac tgactcatcc attcgaacac ggtcagatca
    16081 ttttcaaact catattcgtg gtcctgggcg tagtaaccaa tgcgcgcgtt ttcagaccat
    16141 tttacggtgc cgctgtccgg ttgcagatcg cccaccagcg ttttcagcag cgttgattta
    16201 ccgacgccgt tggtacccag taccgcaagt ttttcaccga cctccagcag caggttgaga
    16261 tttttaaaca gtggaccgtt atcaaaccct ttggtcagac cttccacttc cagcgcgtta
    16321 cggaacagtt tcttatcctg ttcaaaacgg ataaacgggt tctgacggct ggaggctttc
    16381 acctcttcca gtttgatttt atcaatctgg cgcgcgcgcg aagttgcctg gcgagatttc
    16441 gaggcgttgg cgctaaagcg gctaacgaaa gattgcaact cagcaatctg cgctttcttc
    16501 ttggcgttat cggccagcag acgttcacgc gcttgggtcg ccgccgtcat gtactcatcg
    16561 tagttacccg gataaacgcg cagctcgccg taatccagat ccgccatgtg ggtacagacc
    16621 atgttaagga agtgacggtc gtgcgagatg atgatcatgg tgctgtcacg ctcgttcagc
    16681 acctgttcca gccagcgaat ggtgtcgatg tcgaggttgt tggtcggttc gtcgagcagg
    16741 agaatatccg gctcagcaaa cagcgcctgc gccagaagca cacgcagctt ccagccagga
    16801 gcaacttcac tcatcgggcc gtagtgctgt tccactggaa ttcccacgcc aagcaacagt
    16861 tcaccggcgc gagcttccgc agagtaaccg tccatttcgc cgtatttcac ttccagatcg
    16921 gccactttat agccgtcttc ttcactcatt tccggcaaag catagatgcg gtcgcgctcc
    16981 tgcttcactt cccacaactc tttatgcccc atgatcaccg tatccagcac agtgaactct
    17041 tcaaaggcaa actgatcctg acgcagctta ccaatgcgct cgttgggatc gagggaaacg
    17101 ttacccagcg tcggctcaag gtcgccgccg agaatcttca taaaggtgga tttaccacta
    17161 ccgttcgcgc caatcaggcc gtaacggttg ccgccgccga atttgacgga aatgttttca
    17221 aacaacggct tactgccgaa ctgcatggtg acgttactgg aaactaacac aggcgtatcc
    17281 tgaaaagaga tatgacaaac cgcgcattat gccataagtc tgaaagaaga tcctgctcct
    17341 cgcttaaggt ctacactcaa gccaaaggca aaaaatgtga tttcgtacac atctgatttc
    17401 actgtgagct ggaatgaact tataatgcgc ttccaatact ctaatattct caacccaatg
    17461 gcctgccagg cacaaaatct cgcttaacat gaatatgaaa ttgaaaacat tattcgcagc
    17521 ggccttcgct gttgtcggct tttgcagtac cgcctctgcg gtaacttatc ctctgccaac
    17581 cgacgggagt cgcctggttg gtcagaatca ggtgatcacc attcctgaag gtaacactca
    17641 gccgctggag tattttgccg cggagtacca gatggggctt tccaatatga tggaagcgaa
    17701 cccgggtgtg gataccttcc tgccgaaagg cggtactgta ctgaacattc cgcagcagct
    17761 gatcctgccg gataccgttc atgaaggcat cgtcattaac agtgcagaga tgcgtctgta
    17821 ttactatccg aaagggacca acaccgttat cgtgctgccg atcggcattg gtcagttagg
    17881 caaagatacg cctatcaact ggaccaccaa agttgagcat aagaaagcag gcccgacctg
    17941 gacgccgacc gccaaaatgc acgcagagta ccgcgctgcg ggcgaaccgc taccggctgt
    18001 cgttccggca ggtccggata acccgatggg tctgtatgca ctctacatcg gtcgcctgta
    18061 tgctatccat ggcaccaacg ccaacttcgg tatcggcctg cgtgtaagtc atggttgtgt
    18121 gcgtctgcgt aacgaagaca tcaaattcct gttcgagaaa gtaccggtcg gtacccgcgt
    18181 acagtttatt gatgagccgg taaaagcgac caccgagcca gacggcagcc gttatattga
    18241 agtccataac ccgctgtcta ccaccgaagc ccagtttgaa ggtcaggaaa tcgtgccaat
    18301 taccctgacg aagagcgtgc agacagtgac cggtcagcca gatgttgacc aggttgttct
    18361 tgatgaagcg atcaaaaatc gttccgggat gccggttcgc ctgaattaat cgtcaaaacg
    18421 taaaacaaag gcggactcat aatccgcctt ttttatttgc cagaccttac caggccggga
    18481 tcataactaa caaaacgtat cccaccagcg ccgcccacaa cagcatcggt attgaataga
    18541 gatggaaacg ccaccagatg cggcgatcgt tcgccatacg tagcgcaatc aaatttgcca
    18601 gcgatcctgg taataaacca aagcccccca cgtttaccgc ccatgccagt aataaagacg
    18661 gaggtacata attgagcagc aaaatggtac tcggcacgtt actgatcacc tgcgataaac
    18721 cgactgccgt taaccataac ccgggttcag ataaatgact cacgttaccc aacacgcctt
    18781 gcaacgctgg aagctgggtc agtaaatgga cgtcgataaa catcgccata aacaccagca
    18841 gcaacgtcca gtccacactc aacaccacgc gacgcgccag cagcacaaag cccgccgcca
    18901 caatcaccag tccccacagc tcttgtttga actccagcgc cgtcagaaag acgatataca
    18961 gccccagaca attccacacc agccgcggtt tccactccgg tgtttgcacc cccgtatggt
    19021 attgcagcgc ctttccaggg aaacaacacc agcacaacag caggagtgtc agcatcattg
    19081 cgccagccat cggtgccatt tgggcaataa atccggcaaa cgaaagacca gaacgtcccc
    19141 agataagaat attttgcggg ttgccaattg gcgtcagtag cgaaccagcg ttgactgcca
    19201 gcgcctcaaa aataatcagc cgattaaccg ggatctcaca cagtcttttt agcgtgatag
    19261 tcagcggaac aacaataaac agcgcgacat cgttggtcag aaaggtagaa agcagcgccg
    19321 ccgccagcac cataaacatc gccagccgac gctccgtagc aaagcggcgc accattttgc
    19381 gccccagcac atcaaaataa ccgcttaact ccacaccttt ggtcagcagc atcaggccgc
    19441 ttaaggtgat gatggtgtgc cagtcgatag cagcaggcca ggatttcggt gcaaagggca
    19501 cgaaaaagct taatccgata ccaacaagaa ttaataactg aaaaaaacga tcgccttgca
    19561 gcgtgcgtaa aaaaggcagg ctcatttggc accgtgtttc tgggtaaaca aacgaaaagc
    19621 gtcgagcgtc tcttcactaa catggtgctc catgccttcc gcgtcgcgac gggcgatttc
    19681 cggactgacg cccaacacca acaagaaatt ttcgactatc tgatgtcgct cgcggctttc
    19741 ctgcgccagc ttctctcctt ctgccgttaa aaacacgcca cgccagggga tcatttcaat
    19801 cagccccatg gttgccagcc gcttaagcat tttagccacc gtcggttgcg aaactcccag
    19861 acgagcagcc atgtccacct gacgagcttc ccccacttcc ctgatcaagt cagaaatcag
    19921 ctcaacgtaa tcatcaataa gctcgcgccg atgcgcctct ctgacctggc ggaacccttc
    19981 aacgtgctct tccacgttca ctaactgcgt cacttttttt gctattggcg tacctgcgcg
    20041 acgactcatt gtgcttcctc attatggtga cgcatacata gcgttctcat ttcgtgcgca
    20101 cattgtaaac cagagttgcg aaggtacaaa aaattaacgt tttagcaata gctatataat
    20161 atagcctgtg ctatatctgt atgtaatgca atcatccctc aaggatcgac gggattagca
    20221 agtcaggagg tcttatgaat gagttcaaga ggtgtatgcg cgtgtttagt cattctccct
    20281 ttaaagtacg gttaatgctg ctctctatgt tgtgcgatat ggtcaacaac aaaccgcagc
    20341 aagataaacc ttcagataaa tagcggcgtc gcggtacgcc gcttcactcc tgcttttatg
    20401 caggcataac gcgttttggt ctgaaaaaaa ccactttttg tcggatttgc aatccccttc
    20461 gcaaaagatt tgttcgtcag tagttgacct gaacggcggc tcgctctatc ttcttgcagc
    20521 cctgcgtata ttgcggctcg cggatgcgga cccctttccc ctcttcacgc actcttgcag
    20581 gtattgaccc ttgacgccag ggtaagcaca tggcgtttgt tacgatagtg gcatatgaat
    20641 ttaaccctca aagaatcgct tgttacccgt agccgggtat ttagcccgtg gactgcgttc
    20701 tactttttac agtcgctatt aattaacctc ggcttaggtt accccttcag tttgctctac
    20761 accgctgcgt ttacggctat tttgcttttg ctatggcgaa cattgcctcg cgtacaaaaa
    20821 gttctggtcg gtgtcagttc gctggtggcg gcttgttatt tcccttttgc tcaggcctac
    20881 ggcgcgccta atttcaatac attgctggca ttgcactcca ccaatatgga agagtcgacc
    20941 gaaatcctga cgatttttcc gtggtacagc tacctggtcg gcttatttat ttttgcgctc
    21001 ggcgtaatag caatcaggcg aaaaaaagag aatgaaaaag cgcgctggaa taccttcgac
    21061 agcctgtgtc tggtattcag tgtggcgaca ttttttgttg ctcccgtgca aaacctggcc
    21121 tggggtggcg tatttaaact gaaagatact ggctatccgg tatttcgttt tgctaaggat
    21181 gtcatcgtca ataataacga ggtgattgaa gagcaagaac ggatggcaaa actttccgga
    21241 atgaaagata cctggacggt cactgccgtt aagccgaagt atcagaccta tgtggtagtg
    21301 atcggtgaaa gcgcgcgtcg cgatgccctc ggtgcctttg gcggtcactg ggacaatacc
    21361 ccgtttgcca gcagcgttaa cggtttgata tttgctgact acattgctgc cagtggctcc
    21421 acgcagaaat cgcttggctt aacgctcaat cgcgtagtcg atggcaaacc acagtttcag
    21481 gataactttg tcaccctggc aaatcgcgcg ggcttccaga cctggtggtt ttccaaccag
    21541 ggccaaatcg gcgaatacga tactgctatc gccagcatcg ccaaacgtgc agatgaagtg
    21601 tacttcctga aagaaggtaa ttttgaagca gataaaaaca cgaaagacga agcgttactg
    21661 gatatgaccg ctcaagtgct ggcgcaagag cactcgcaac cgcagctgat tgttctgcat
    21721 ctgatgggct cgcatccgca ggcctgcgac aggacacaag gcaaatacga aacctttgtg
    21781 caatcgaaag aaacgtcgtg ctatctctat accatgacgc aaacggacga tttactgcgc
    21841 aagctgtacg atcagttacg caacagcggc agcagcttct ctctggttta cttttctgac
    21901 cacggtctgg cctttaaaga gcgcggcaaa gacgtgcaat accttgccca tgatgataaa
    21961 tatcagcaaa atttccaggt gccttttatg gtcatttcca gcggcgataa agcgcatcgg
    22021 gtgattaaag cccgccgctc agccaatgac ttcttaggct ttttctcgca gtggacggga
    22081 attaaagcga aggaaataaa tatcaaatac ccgtttatat ctgagaagaa agccgggccg
    22141 atatacatca ccaacttcca gttacagaag gtggattaca accatctcgg aaccgatatt
    22201 ttcgacccga aaccttaaaa acaaaaatcc gccccgagag gcggattttt atatcaccaa
    22261 agtgattaga agcggtaacc aacaccggca atccaggtgc ctacgtcaac gctacgaata
    22321 cggctctgct cgtaagagaa gtccagagca acgttttcca tcgggttgaa ctgcagacca
    22381 gcaccgtagg agaaaccgta gtcgctggtg tcgtgtttgt aggtcgggta ttcagtggtc
    22441 tggaatttac cataacccac acccactaca ccgtagatgc ttgcccagtc gttaatgcgg
    22501 taagccggac cagcagtgat gccgtagtac tggtttttgt tgtagtcacc agagcttgca
    22561 gtacggcttt tctcggtgta agtgaaagaa ccgatcacac ccagcgggct gttgtcttct
    22621 tcatagcggt atttcaggtt gaaaccgccc attttgttca tttggccctg agcgtcgctc
    22681 tgtgcgtaac cgccagttac ggtagaagtc gcagctacgg aagtacctgc ggtgaaagcc
    22741 agaactgcgg ccagtgctga aagacatgca atttttttca taaccacctc aaatgtgatt
    22801 caaataagtc ctaagtttta aatatatcaa aaattaatgg gaaactcttc gcgat
//
LOCUS       DAZPAB010000047        22716 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZPAB010000047 DAZPAB010000000
VERSION     DAZPAB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.047"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            25..1254
                     /locus_tag="ACHUPG_001648"
     CDS             25..1254
                     /locus_tag="ACHUPG_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0861311.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGEKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            1629..1817
                     /locus_tag="ACHUPG_001649"
     CDS             1629..1817
                     /locus_tag="ACHUPG_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0861312.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(1867..2196)
                     /locus_tag="ACHUPG_001650"
     CDS             complement(1867..2196)
                     /locus_tag="ACHUPG_001650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861313.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(2321..2500)
                     /locus_tag="ACHUPG_001651"
     CDS             complement(2321..2500)
                     /locus_tag="ACHUPG_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861314.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            2630..2830
                     /locus_tag="ACHUPG_001652"
     CDS             2630..2830
                     /locus_tag="ACHUPG_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFR0861315.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            2820..3041
                     /locus_tag="ACHUPG_001653"
     CDS             2820..3041
                     /locus_tag="ACHUPG_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861316.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            3034..3254
                     /locus_tag="ACHUPG_001654"
                     /pseudo
     CDS             3034..3254
                     /locus_tag="ACHUPG_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3256..3489
                     /locus_tag="ACHUPG_001655"
     CDS             3256..3489
                     /locus_tag="ACHUPG_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861317.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            3495..3794
                     /locus_tag="ACHUPG_001656"
     CDS             3495..3794
                     /locus_tag="ACHUPG_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFR0861318.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            3791..5539
                     /locus_tag="ACHUPG_001657"
     CDS             3791..5539
                     /locus_tag="ACHUPG_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFR0861319.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            5819..6097
                     /locus_tag="ACHUPG_001658"
     CDS             5819..6097
                     /locus_tag="ACHUPG_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861320.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            6094..6399
                     /locus_tag="ACHUPG_001659"
     CDS             6094..6399
                     /locus_tag="ACHUPG_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861321.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            6409..6483
                     /locus_tag="ACHUPG_001660"
                     /pseudo
     CDS             6409..6483
                     /locus_tag="ACHUPG_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6558..6671
                     /locus_tag="ACHUPG_001661"
     CDS             6558..6671
                     /locus_tag="ACHUPG_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR0861322.1"
                     /translation="MSRVGISEPHHAAGAIRLFLRHLKRYGALLAFRHGPA"
     gene            6712..6936
                     /locus_tag="ACHUPG_001662"
     CDS             6712..6936
                     /locus_tag="ACHUPG_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861323.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            7237..8394
                     /locus_tag="ACHUPG_001663"
                     /pseudo
     CDS             7237..8394
                     /locus_tag="ACHUPG_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            8434..9006
                     /locus_tag="ACHUPG_001664"
     CDS             8434..9006
                     /locus_tag="ACHUPG_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFR0861324.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            9008..10219
                     /locus_tag="ACHUPG_001665"
     CDS             9008..10219
                     /locus_tag="ACHUPG_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR0861325.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            10216..10554
                     /locus_tag="ACHUPG_001666"
     CDS             10216..10554
                     /locus_tag="ACHUPG_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFR0861326.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            10551..10847
                     /locus_tag="ACHUPG_001667"
     CDS             10551..10847
                     /locus_tag="ACHUPG_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFR0861327.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            10847..11287
                     /locus_tag="ACHUPG_001668"
     CDS             10847..11287
                     /locus_tag="ACHUPG_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR0861328.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            11271..11453
                     /locus_tag="ACHUPG_001669"
     CDS             11271..11453
                     /locus_tag="ACHUPG_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861329.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            11509..11835
                     /locus_tag="ACHUPG_001670"
     CDS             11509..11835
                     /locus_tag="ACHUPG_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR0861330.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            11819..13480
                     /locus_tag="ACHUPG_001671"
     CDS             11819..13480
                     /locus_tag="ACHUPG_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR0861331.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            13494..13775
                     /locus_tag="ACHUPG_001672"
     CDS             13494..13775
                     /locus_tag="ACHUPG_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861332.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(14572..16032)
                     /gene="phoQ"
                     /locus_tag="ACHUPG_001673"
     CDS             complement(14572..16032)
                     /gene="phoQ"
                     /locus_tag="ACHUPG_001673"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR0861333.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(16032..16703)
                     /gene="phoP"
                     /locus_tag="ACHUPG_001674"
     CDS             complement(16032..16703)
                     /gene="phoP"
                     /locus_tag="ACHUPG_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR0861334.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(16872..18242)
                     /gene="purB"
                     /locus_tag="ACHUPG_001675"
     CDS             complement(16872..18242)
                     /gene="purB"
                     /locus_tag="ACHUPG_001675"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR0861335.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(18246..18887)
                     /gene="hflD"
                     /locus_tag="ACHUPG_001676"
     CDS             complement(18246..18887)
                     /gene="hflD"
                     /locus_tag="ACHUPG_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR0861336.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(18923..20029)
                     /gene="mnmA"
                     /locus_tag="ACHUPG_001677"
                     /gene_synonym="trmU"
     CDS             complement(18923..20029)
                     /gene="mnmA"
                     /locus_tag="ACHUPG_001677"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR0861337.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(20083..20544)
                     /gene="nudJ"
                     /locus_tag="ACHUPG_001678"
     CDS             complement(20083..20544)
                     /gene="nudJ"
                     /locus_tag="ACHUPG_001678"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR0861338.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(20554..21207)
                     /gene="rluE"
                     /locus_tag="ACHUPG_001679"
     CDS             complement(20554..21207)
                     /gene="rluE"
                     /locus_tag="ACHUPG_001679"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR0861339.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            21379..22629
                     /gene="icd"
                     /locus_tag="ACHUPG_001680"
     CDS             21379..22629
                     /gene="icd"
                     /locus_tag="ACHUPG_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR0861340.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         5987 a         5518 c         6012 g         5199 t
ORIGIN      
        1 attgtgatga ccggaatcaa aattatgagc agagcactta acaaactgag cgatacacag
       61 ctgaggaaaa tcaacggcac acccgcccaa aaaacagcct ttcttaatga cggtggaaac
      121 ctgagcgtca ggcattcaac cagtggcctt ttaacctggt atttcactta cagggccgga
      181 acgggaaggg gggcaccacc ggaacgcatt aagctgggaa attatcctga tctgagcctg
      241 aaatcagcca gggaaaaagc cgcccagtgt cgcgcatggc tggcagaggg gaaaaatcca
      301 cgtcatgagc ttaattacac cgtacaggaa gcgttaaagc cggtaacggt tggcgatgcg
      361 ctcacctact ggcttgagtc gtacgcaaag gaaaaccgcg tggattatgc cgccctgaaa
      421 aagcgcctta ataatcacgt aatacagcac attggtgcta tgccgctgga taaatgcgag
      481 ctacggcact ggctggcctg ttttgaccag gtggcaaagc gaacgcctgt tactgccgga
      541 ttcttgctac agacgtgcaa acaggcgctt aagttctgcc ggaggcggcg ctatgcaatc
      601 agcaacgttc ttgatgatat gagtgtggcg gacgttgggg aaaaaccgga tataagcgag
      661 cgtgtcttaa gcaccaaaga actgggcgaa ttattgcagg cactggacaa aaaaatattc
      721 tccccctact acatcgcgtt aatccgcctc ctgattgtgt tcggatgccg gacggtagaa
      781 ctgaggttat cggagatcag cgagtgggat tttaccgaaa tgctctggac cgtgccgaaa
      841 gaacacagca aaacgaaggt ggcaatattc cggcccatac cggaagcaat actgccgttc
      901 gtcacgcagc tggtggagca gaacaggcac acgggcttat tgctggggga agtgaaacag
      961 gaaacaagcg tgtcgcagta cggcagatta gcgcacagga ggctaaatca ccctcactgg
     1021 tcactacatg acatccggcg cacctttaca acgatgctga acgatttagg cgtggatcct
     1081 cacgtcgtgg agcagcttac aggccaccag atgccaggaa tgcagcgagt ttataatcat
     1141 tcccgttatc tggatgctaa acgcaatgcg ctggatatgt ggacggagcg gttagggata
     1201 ctggcgggaa cacatgaaaa cgtaaccacg ctaccagtag ccagaagaaa ataatttttt
     1261 tcgtgttttt tcagtatgcg catactggat atgcgaacag atacaacgtg caacaatgag
     1321 caacaatgcg gaacaactac gaacaagagg tgaaaaagtg tacagattta taagtagctg
     1381 atttttaatg tgttactgat tttttataca ctagcgaatc actctttaaa tagcgagaaa
     1441 aaaggcatga aacaatattt tatgcctttg tattaacatg aatttaaagg attttatcct
     1501 gattattcct ctttctgtaa accatacggc ctcaacattt gccaactctg aatcatcctg
     1561 acacaatcag acacatccca acgcaataga acacgaaata actctttaag aaacgaaagg
     1621 gggcaatagt gttaagcact gatcggttta tacgtgaaaa agaatgcgaa aagctaacag
     1681 gccttagccg ctcatgccgc taccgcctgg aaaaggccgg acaattccca tcacgtcgta
     1741 aacttggcgg tcgttccgtt ggctggtctt tatccgaggt tctggcctgg aaggatagct
     1801 gcaaggcagt tcattaatca cgctggcggc acgcagccgc cacacatcaa tcatctgaac
     1861 acagagctat aaccatgaag attgaatata cgccagaacg tgggcgggga ttcgttcgcc
     1921 ctggtgaaac tggaaaacca caaaattggg gtttttcagg cataaaaaaa gcggccccga
     1981 aatggagccg cctttctgaa cagataaccc gctgcgccgt ttgtgtgtgt gatcccaaac
     2041 ataagcacgg ggatgatagc cgctatcagg ctggtgggca atgcaatcag tctggttcag
     2101 tgcgttgcca taactgcgaa gttctctcct ttgatgagta ttctttaagg tacttctcaa
     2161 gggcaaaaga acacggcgcg aatcgttctg attcatgctc tttctttctg cgtcgtcttt
     2221 ttcgtgcagg tgatgttttt tcggttgatt cttcgttggt cattgtgtgc gcctgtaaag
     2281 caatgcgccg tagtacctca caccacggcg ctgatagttt ttatcctttg ggttctatgc
     2341 cgcgttgctg taattctttg cggattattc gctttatcca ggcagcaaga gaatcatcgc
     2401 cgtcctgttg ttgtgccttt tccatctttt cccgtaagtc aggatctaaa cggaactgga
     2461 acggaggatt accacgcctt tcgtttttgt gtgttgacac gttaattaca cctcaggtaa
     2521 tgtatttatg tgtagtgaca cattacacac aatcaacaca aaaagcaaag ccccgcactg
     2581 taggagcagt aacggggctt ctaaccaaca acgtaaacta ggagccgtta tggttgccgt
     2641 aaatcatata ccacaccttg tacacacaca aacggccttt gtgtggcgtt ttctggcact
     2701 gagtgccgga gaatcccaaa tcatccacgt aaccgcttgg acggaacgcg aagcgcgtag
     2761 ccgttgcccg tccggttgtg ttgctgtatt cgccgcccgt attcgtcagg gggaaaccta
     2821 tgcacaataa aaccacaccg aacgcagcag ccgccgcact cactacgctg atgcacgcgc
     2881 ttattgatat tgaatgcacg gcagagcttg cgcagggaga agaacagaaa gaccggacac
     2941 agttcgccct ggaatgtatc cgatacatcg caacgcggtc actgaatgac gctaaaaata
     3001 ttcttgttgc cgattgtgaa aatggggggg gttatgcgtg atgatcgttt taattccctg
     3061 aaacaggaat tttccggcgt tcctgatgat gcggctgatg cgctttcgtc aatgccagaa
     3121 cttattagag cggctttttt cttactttcc acgagagaat ataaatcaac gggcttgatg
     3181 tactgaatat cgccgccgat tatgctgatt tcgtgacaga ggttatttta agaaaaacga
     3241 cggacgggga ttaatatgcg tgatatttac cacgaaacaa tagaccgcgc atttcttgca
     3301 ctttctcaca gtgaaaacat gctggaaata ttgcgcatat ggcttgaaac acttggcgac
     3361 aatgaacgcg acaaacaaaa atcaagaatt gccacggcat taataacgct tcttgagcct
     3421 gtaataatgg aactgcaaga aatagatcta ttgcacgaca gatataaaga acaacacacc
     3481 ggagaataaa aataatgaaa cttaaatatt ctggcttaac tgccagtggc aacactcacc
     3541 ctaaatttac gcgcggtgat atttaccgcg accagtacgg cggcatggta acgattaaag
     3601 gcgtggcaga acggcgcatc acctaccgcc gtgaaggtta cgaatatgat tgcgtgatgc
     3661 ctgtttatca attccggcgt gatttcacgc tggtacaggc agcgccccgc agtaaaccaa
     3721 ccagcaggga gaaagcacgc gccaatattc aggaaataaa aaagatgctt aacgtattca
     3781 ggggtaaaaa atgaaactgg caccgaacgt aaaaaaacag ccacgcggaa taaaacacaa
     3841 agacacagag gtaattatct ttgcgggtag tgatgcctgg tcgcacgcga aacaatggca
     3901 ggagcaggat ggccccgcat ccggcgataa tgtgccgcct gtgtggcttg ggccaaatca
     3961 gcttgccgaa cttgatgcac tgaaaattgt tccggatgga aaaaaacgcg taaggctgta
     4021 ccaggccgga gaactggatt tggtggagac caaaaagatt ggtcagaagc tggcggcggc
     4081 agatattcag gacgcaaatt tttaccccga aggaatgcac gtccagaagt gtgaaaactg
     4141 gcggcgctat ctgaatgctg agcgtgaaaa tattgccgca gggcttacca tgccggagca
     4201 gaaaaatacg caactggcac aaatggcaga cagtgagcgc gcgcagctgc ttgccgagcg
     4261 ctttgatggc gtttgtgtgc atcaggaaag tgaaatcgtt cacgtatggc gcggcggggt
     4321 atggtgtccg gtcagcacaa tggaacttag ccgcgaaatg gtggtgatct attcagagca
     4381 cagggccact ttcagcaagc gcgtaatcaa taacgccgtg gaagcgttaa aagttattgc
     4441 cgaaccaatg ggcgagccgt ccggcgattt gctgccgttc gccaatggtg cgcttgacct
     4501 gaaaacgggg gaattttccc cgcacacgcc ggagaactgg atcaccacgc acaacggcat
     4561 tgagtacacg ccaccagcac caggggagaa catccgcgat aacgcgctaa actttcataa
     4621 atggcttgag cacgcagccg gaaaagacca gcgcaagatg atgcgtatat gtgccgcgct
     4681 gtacatgatt atggcgaacc ggtacgactg gcagatgttt attgaggcca ccggagacgg
     4741 cgggagcggt aaaagtacat tcacacacat agccagcctt ctggcaggga aacagaacac
     4801 ggtaagcgct gaaatgacat cgcttgatga tgctggtgga cgtgcgcagg ttgtcgggag
     4861 tcgtcttatc gtcctggctg accagccgaa atatacaggc gaaggaacgg gcatcaagaa
     4921 aatcacgggc ggtgaccccg tggaaattaa cccgaaatac gaaaagcgtt tcacggctgt
     4981 aatcagggcg gtggtgctgg cgaccaacaa taacccgatg atattcaccg aacgggccgg
     5041 aggtgtggca cgtcgtcgcg tgattttccg tttcgacaat atcgttagtg aggccgaaaa
     5101 agacagggag ctaccggaaa agattgcggc tgaaatccct gtcattatcc gccgattgct
     5161 ggcgaacttt accgactctg agaaagcacg ggctttacta ctggaacagc gtgacggtga
     5221 tgaagctctg gcaataaagc aacagacgga tccggttatt gagttttgcc agttcctgaa
     5281 ttttctggag gaagcacgcg gcctgatgat gggcggcggt ggcgattcag tgaagtacac
     5341 gaccagaaac agcctttacc gcgtctatct ggcgtttatg gcgtacgcag gcaggagcaa
     5401 accgctaaac gtaaatgact ttggcaaggc tatgaagcca gccgcgaaag tttacggaca
     5461 tgaatatatt acgcggaaag ttaagggagt aacgcagact aacgcaataa caacagacga
     5521 gtgcgacgcg tttttataat tttttgtaat tgctgtctac ctgagtaaag aaaaatacat
     5581 ttaattcagt gcattaagtt aggtagatag ccttttttta ctgtctaccc actatctacc
     5641 ctctctacct gattttacct gaatcagaca gggaggtaga cacggggtag atagtggata
     5701 aaagcactct accccgctga aagcagcgcc attactggca tgagggccac taaggtagat
     5761 aaggtagaca aggggaggca caactcaaaa ctttttaaac gagggggtaa aaataaatat
     5821 gcacacttca gggaaattta ataaatcatt caaaaaacgc agagacagaa cagaaccgaa
     5881 atatcgcgcg ttagacatga cagagcacgc tttaaaggtg gcaatcagaa cgatagaccg
     5941 ccacgcgggg gaaggatacg cgaaagcaca tcccgaactg ataagcgcat tcatgaccac
     6001 gacggcggca aattttgcca cgctgacaga gcgggagatt gccgaagcgg aacaggtaac
     6061 aacaatcaac gttaaaaccg gagaggtgga atcatgagcg aaccaaaatt tggtgaaaaa
     6121 ttttacaagc acaacggacg gatcacgatt ctgcaaataa gcgcagctac accaggctgg
     6181 tgggttgaaa cagacgaagg atcctcaccc gtggcatcat gggagctttg tgtgcttagt
     6241 taccctgacc gcgatgtgta tcaggatatt ctgcctgtga tatccactga caaaggcatg
     6301 aaacccgtag acatcaaaaa catggggttt cagtgcgtaa tgctcactga aaaaatgatg
     6361 gaagagatga aaaagaacaa tgccggatcg cttcactgag gtggcaatat gacagcacaa
     6421 attgcagctt acgggcagct ggtgggcgac ccgctggtaa aacagagcag caagggcaca
     6481 ccggagccgc caacggagaa ctacgcagac ctgagaaatg cagtaaatcg cacttacgca
     6541 gacatggggc tgagcaaatt agcagagtag gaatatcaga accacaccac gcagccggag
     6601 caatccggct ttttttgcgt catttgaaac ggtatggagc actactggct tttcgtcacg
     6661 gtccggcata gttactatct gaaacaaaca gacacaacag aggaaaacgc aatgccgatg
     6721 aaatttgatg agatacttaa acagcgtgat aaataccatg ctgacaacat ggagacgatg
     6781 aatatcactg attaccgcgc atttctggaa acgggggcgc tgattgaaaa ggatcatcat
     6841 ggttttgttc gttgcgcgct atccggtgaa atgctggcag taaatcctga acagacagat
     6901 gcattgatag aatttctgaa agggatcaga gactgagcac ccatacagca catacagccg
     6961 gagcaatccg gctttttgtt attttttgta aattatttgt tcgtggttgt tccacgttgt
     7021 tcactgagcg gatcggcata ttttacccga actgaatcat gattattctc gcccgtggtg
     7081 ccaggacgct ggggccactt tcccgcctgt taatgtgctc gccaatattc attaccaggc
     7141 gggaaaacga tcggtgcgat tactgatttc cttatgaaaa acggttgagt gtttgccgcg
     7201 tcctggagtt ccttacttaa ccccaggact ttttttatgc agagaataat cgaattacgc
     7261 cagcagaaaa ccgccattaa aaatcagatg cgcgacatgc tggagaacgc tgaaaaagaa
     7321 aaccgcagtc ttaacgatgc tgagggcgca aaatttgacg aattacgcgc taaagctgaa
     7381 tccctcgata aagacatttc ccgccttgaa gccattgcag acgaagagcg cagcaagcca
     7441 ggtaaaagca gccagaccac tgaccccgca gaactacgta actacattct gaccggtgaa
     7501 acccgcgcat taagtacagg cgttcccgct gatggtggtt ataccgttat ccctgaactg
     7561 aacaccgaaa tcatgcgaat gctggcggat gaatccacca tgcgtcgcat ctgtaccgtg
     7621 aagaaaatca gcagcaacga gtttaagcag cttgtttccg ctggcggtgc gaccgttaac
     7681 cacggtgaag agggtaaggc acgcgaacag accagcaccc cgcagattaa cgaggtgagc
     7741 attaagctgt atccggtcta tgcgtacccg cgcaccacac aggaaatcgt ggatttttcc
     7801 gatgtggaca tcctttcatg gctgacgggt gagattggcg acaccttcac ggaaaccgaa
     7861 gaaagcgatc tggttgtggg cgacggtgac aaaaaagcaa aaggtttttt atccgtaccc
     7921 cgtgcagaga agaacgacaa agagcgtgat tttggtacgt tgcaggtaat taaaccttcc
     7981 gaatctctgg cgtggacatc tgcggacccg ctgatcgacc tgaaatttgc attacgtaaa
     8041 aaataccgca aaaacgcggt ctgagtggtt aactccacga cggcggcaaa acttcagaag
     8101 gtgaagaacg cgaacggtga ttatatctgg cgcgaccgtt tacaggcggg tgatcctgat
     8161 acgttgctgg gccttccggt cgaatatctg gagtttatgc ctgataacgt tattgccctg
     8221 ggtgacttca aacgcggtta ctacattgtt gatcacgaaa caggtgttcg caccagaccg
     8281 gacaacctga cagaaccagg attcatcaaa attttcacgc aaaaatattt aggcggtggc
     8341 gtggtggatt cgaacgcgat caagattctg gcactgccgg aagacgacga ttaacagcat
     8401 acagaagggg ctaaaaagcc cctttagtgt tttatgggtg aaaaaattat gaagagtatg
     8461 gaaatccggt catcggaaat caccaccagc ggagccggta cgctgacggg ctacgttgtt
     8521 cgctgggata agctttcaga actgttatgg ggtgagtttt acgaaaaatt ccagcggggg
     8581 gcgtttactg aatggcttgc ggcgggtaat gacgttcgcg gcctgtatga gcatgaccac
     8641 agcatgttac tggggcgcac ccgttccggc acgctgaaac tggaagagga cgaaacaggg
     8701 ttacgctttg aactgacccc accggatacc agtacagggc gcgacgttat cgaactggtt
     8761 aagcgtgggg acatctcggg gatgagcttt ggctttcgct cccgtaagga tgtatgggat
     8821 accacaacag atccatgcgt gcgcaccgtg ctggtggcgg aactgtacga aattaccgtt
     8881 acatcggtac cggcttaccc cgattccggc gtggagctgg cccgccgttc cctgtatgag
     8941 cagcaccccg aaaaaatgcc gcgtgcggat aatcgccgct ggtgggcgga tttagcgggg
     9001 gtgtgatatg tggcctttca gacgaaaaaa agagcagcgc agcatgacgc ttgatgaatt
     9061 tatggcgctg gccggcacat cgaacacggg ggcgggtgag tacgtatcat ctgggacagc
     9121 ggaatcactc cccgccgtca tgaacgccgt cacggtcatc tctgacgcgg tggctaccat
     9181 gccgtgttac ctgtacctgg tacgcaatga gaaggggaag gaggcccgcg aatggcttga
     9241 ttctcatccg gtcgatcaca tcctcaacga gcgcccgaac gcgtggcaga caccctacca
     9301 gtttaagcga atgatgatcc gctactgcct gttaagtggt aatgcttatg cggttattca
     9361 gtgggggcgt gatggttttc cggcggcttt acatccttac ccgccgcagt cggtgaacgt
     9421 ggagcagaca ggtgatcata actggcgcta ctgcatcact gacgcctaca ccggaaacat
     9481 ccgcaactat ttaccgtggg aggttctcca ccttcgttac tccacggatg acggttttat
     9541 ggggcgctca cctgtaacca tctgccgcga atcgctgggg cttgggctgg cccaacaacg
     9601 ccacggcgcg agcgtgatgc gtgatggcat gatggcggca ggggttatca cgtcaggcga
     9661 atggctggac ggcgtgaagg gcaaacaggc attagccgca ctggaacgct acaaaggggc
     9721 cagaaacgcc ggaaaaacgc ccatccttga aggggggatg agctatcagc agctgggcat
     9781 gagtaatcag gatgccgaat ggctggcctc ccgtcgcttc accattgaag acatcgcccg
     9841 aatgttcaac gtctcgccga tttttttgca ggaatacagc aacagcacct acagcaactt
     9901 cagcgaggca agccgcgcat ttctcaccat gacgatgcgc ccgtggctgg cgaactttga
     9961 gcagcagata aaaaacgcct tgctggtggc ctcgcctgta cctggtatcc ggtatcaggt
    10021 ggagtttgac agcgcggacc tgttacgggc cacacctggc gaacgctttg ccacctatga
    10081 gcgcggcata aaatccgacg ttatgtgtcc gaacgaagcc cgcgaacgtg aagggctgtc
    10141 accgcgtgat ggtggtgatg agttcagcca ggcatggaaa caggaagtaa aaatcagcga
    10201 gggagaaaaa ccggaatgaa catagggcga ctgcgtgaca ggataacgat tcagaccctg
    10261 aaacagacca gggatataac cggcgaaata ctcgaaacgt gggaggacgg tcacacactc
    10321 tgggcaagcg tgaacatggt cagcagcaag gaggccattt catcgggcgc agagctggcg
    10381 attggtaccg taaggatctg gatacggtac cggaaggaca tcaacgccac cagccggata
    10441 aaggtcagta cggggccgct ggcggggcgt gtactgaata tcatcgggca gccgctgccg
    10501 gatgccgcca ggacacgcct tgaaattctt tgtcgtgagg gcgcggaaaa atgacagaag
    10561 aacttatcac cctggaagaa gtgaaactcc attgccgcat cgatggcgac gatgaagacc
    10621 agttaatcag cggatacatt gccgcatcgc ttgaggcgtg ccagatacac ataggcaggc
    10681 gctttgatga cgggctggcg ttcacgccag ccataaagat tggctgcatg atgtttatcg
    10741 ctcacctgta tgaaaatcgc cagattgtcg cggataacgc aaaaacacgc gtacccatga
    10801 cgataggcgc actctggacg gcttaccgtg atgtgggggg gtactgatgc cgtggcaacc
    10861 attaagacga tgcacagaac caggctgtaa caggcgcgtt aaatccggca agtgtgagga
    10921 gcacaggcgg gcggcatggc gtgcagagga tgccagacga ggacaccgcc gcgcgcgtgg
    10981 atattccaga cagtgggaca aataccgcgc catgtatctg agtaaaaacc cgttatgcgt
    11041 gcgatgcctt gagaagggga tatatacgcc cgccgtggtg gtggatcaca ttatcccgat
    11101 taatggcggt gatgatgttc tcttctggcc cgaatggaac catcaaccat tgtgccaggc
    11161 gtgtcataac cagaaaacga aatggcttga tccggcaaca aaaagcaagc gtgccgcagg
    11221 tggatttcgt gaagaggaag aacgggctgc taaccgcaat aactggatgt atgacgctga
    11281 tgaatgagcg ggaacaaaac cgccttatcc gtggacttat aaggcagcgt gacgcatgga
    11341 agacacagga aacagggcat aaagataaag cgtcaggacg cgcagaacgc atcacagcga
    11401 agcgattaac cgaccgtgac cgcgaggtta tggaatgttt ccgcaatcgc tgatgaggct
    11461 gtatgacggg gtggggggcg ttttcaggac aaacccgacc ccgcctacct ggatgatatc
    11521 gccgtgaagc agtggcggga aaaatcgcgg cagcttgcgg aacggggaga cctgaccccc
    11581 gccgactgga gcaatctgga actgtattgc gtcaactact ccatttaccg gaaagccgtt
    11641 gcagaccttg cggcgcgcgg gttcagcatt gttaacagtc agggcggcga gagcagaaac
    11701 cccgcattaa gcgcaaaatc cgacgctgaa agagtgatga taaaaatggc ctccttgctc
    11761 ggttttgacc cgataagccg ccgtaaaaat ccaccggaaa cagaagaaga ggacgagctt
    11821 gaccgcctgg aataagtacg cagaagacgt aaaaacgggc aaaattccgg cctgtaaacg
    11881 gttaaaacag gctgttaaac ggtacttttc ggaccttaaa agcccccttt acacgttcga
    11941 tcgtgaggtt gtggagcggt ttattgcctt ttccagggtg tgcccgcacg taaaaggacc
    12001 gatgcgtggc agacccattg agctggagcc gtggcagcag ttcgcctttg cgtgcatcct
    12061 cggctttaag gttaaggcca ccggacggcg caaatacacg agcgccttta tcgaagtgcc
    12121 gcgcaaaaat gcgaaatcca cgaccgccgc gattctggct aactggtttc tgattatgga
    12181 gaacggtcag caggatatct acaccgcagc ggtgagccgt gaccaggcgc ggatcgtgtt
    12241 tgataatgcg cgtcagatgt gccttttatc ccgaccgtta cgcaggcggg tgaatattca
    12301 ggcgcataag gtgatacacc cgaaaagcaa cagcctgtta aagccgctgg cagcaaaagc
    12361 ggcaaccatc gaggggacaa accccagtct ttccatcgtg gacgaatacc acctacaccc
    12421 cgataacggc gtttattccg cgcttgagct ggggatgggc gcacgtcccg aagggctgtt
    12481 atttgccatt accacatcgg gcagtaacgt cgtttcagcc tgtaaacagc actatgatta
    12541 ttgctgccag atcctggacg gcgaagaggt taacgattca atttttgtac tgatttacga
    12601 actggacgac gaaaacgagg ttgatgatcc ggcgatgtgg ataaaggcga accctaatat
    12661 tgatgtttcc gtggatcgtg aaaaactggc ctcaaccatc cagaaagcgc ggggtattcc
    12721 gtcgcagtgg gtggagatga tgacaaagcg atttaatatc tggtgtcagg ggactacccc
    12781 gtggatgggt aatggtgcat gggctgagtg tgccggaacg ttcacggagg aagatttaca
    12841 cggtcaggag tgttacgcgg ggctggactt atcatcaacc agcgatattt ccagcgtatg
    12901 ctatgccttt ccggtcggta aaaccattat gctgatttcc cgtcattatc tgccggagtt
    12961 ccagctacag aaccccgcca ataaaaaccg cgctgtctat cgccagtggg ctaaagcggg
    13021 ctggatacgc acaacgccag gtgactgcat tgattacgac cggatcagag atgacatcat
    13081 gcaggatgcg gagaaattta atatcaggct ggtgggcttt gatacgtgga acgccacgca
    13141 tctcaggacg caattacagg gggctggttt tgaggtggag ccgttcccgc aaacctacct
    13201 cagattcagt ccggcggcga aatcgttcga agtttttgtt aaccgcaggg tgattgtgca
    13261 tcgtggcgat ccggtgttgt cctggtcgat gagtaacgtc gtgatgcaga gtgacgcgaa
    13321 cgccaatatc aagccgaaca agaaaaaatc accgaacaag atagacccga gcgtagcggc
    13381 gctgatggcg tttggtacat tccaggcaga gcatgaggat tttgcttttg atataagcga
    13441 cagccaccgc cagaaactgg aagaatttag cggggtgtaa tgaggtcagc agcatgacag
    13501 aagccgaaat actgcgatta atccgccgcg tctctggaat cagccagcag gctgacgaac
    13561 agaccacgca gccggacagc gtgacagccg aaaattatgc gcgtgttgtt gctgaggtga
    13621 tgcgccgtga tggtatccag cttaatgatg cggatatgcg cgacatacgg atccgcgttc
    13681 tggaaatgct ggcctacaat cgccgcgttg aactgtatcg ggagaaggag aaaatcacgt
    13741 accactggaa gaagccggag cggttacggc ggtaactcac tgagattaaa gcaaagcgca
    13801 aaattgcgct ggctacatcc ctcacatctg aggactgtta accagcaggc cggaatatat
    13861 tcagaaacta cgaaattttc gcagtttaat tactcgtcag cttaatcgct aacccgctct
    13921 gatattttcg gaaacctcaa tttgaggaag tcggcgaggt aactgtttga tattcccgat
    13981 agcgcaaaga tgcgcggtcg ggcagaatga ccaccacctc aatcgtgacg gagagtgaca
    14041 accagtgaca caccgtgaca aaaagacggt ttaaccgttc attaaaaatc gacgtgattt
    14101 tgtgctttcc ggatgacgtg gccaacgtca ttttttagcg aaaaattctg attaaggtca
    14161 ctggctgaac ggtcataatg accacgcaga ccatcgaggc aaccatagcg atcatcgtta
    14221 cggttggagc ttttccgcca ttggtggaaa aggtgcacat ctggatcatc attacggtga
    14281 tcaagggtat gtaaaaccca ccagcctgat taaaggttaa ccggaaaaaa aatccggtag
    14341 ccccgttatg gggatcccca tatcgacatt aacgcccctc atgaattggt gcgcttcccc
    14401 ctggaaagat tacccgcctg tatatttctt gtgtctattt gttccacgtt gtttcataca
    14461 gtgcaccgaa cggtgtagtt aatggtgtag tcaatttgcg atttttggca ctttttcaat
    14521 gaaaattgtt tgatcgaatc aatgacttga tgtagtggta aaaggacata tttattcatc
    14581 tttcggcgca gaatgctggc gaccaaaaat cacctccatc cgcgcaccgc ccagcatgct
    14641 ctctccggcg acgattttac cctcatattg ctcggtgatt tcgcgggcta ccgccagccc
    14701 tacaccttgc ccagggcgta aagtatcaac ccgttgacca cggtcgaaaa tgacctctcg
    14761 cttgcttaat ggaataccgg ggccatcatc ctcgaccaca atatagagat gctcgtcggt
    14821 ttgccttgca gaaatttcga caaactcgag gcaatattta caggcattat ccagcacgtt
    14881 gcccatcacc tcgacaaaat cgttctgctc accgacaaag ctgatctctg gcgaaatatc
    14941 gagagagata ttgacccctt tgcgttgata cactttgttc agcgctgagg tgagattgtc
    15001 cagcagtggg gcgaccggat gcagctcgcg gctgagcaat gtcccgccgc gcatactggc
    15061 acgatgcagg tagtagccaa tttgctgtga aatgcggctg atttgctcca gcattaccgg
    15121 ctcagcatca ctgacgctca tcttttcact acgcagagaa cgcagcgtac tttgcagcac
    15181 cgccagtggc gttttcagac tatgggtcag gtcggtgagc gtcgtacggt atttgtcgta
    15241 acgttcgcgt tcacttttta acaatcggtt caggtttcgt accagactgg tcagttctcg
    15301 cgttgtggct ggattgagca attcgcggtt atgttcttcc agttcgcgga cttcttttgc
    15361 cagggcttcg atggggcgta aactccacca ggcggcgacc cacagcagcg ggatcactaa
    15421 cagcagattg gctgagagca catagataaa ccagctccag accatatagg aactttttag
    15481 ctccaccgga atggtatcca ccaccacaat ggttaatttt ggcatccgcg atgttgccgg
    15541 gtagacgttt accgccaccg agtgggtcat ctccgcgtcg tcatcatctt cccgcacttc
    15601 ctgcaactgt tgctgtatcg aatgatctcc actcagcaag aggctggtat cgttaacatc
    15661 cgcttcaatt tcatgaaaac cattcgattt cagccagtca ggctggatca ttttcatcag
    15721 ccagggcacg tcacgttgcg cccataaaag ctgcccgttc tcatcataaa ttagcgtcat
    15781 ggtggggctt tgcttgtcga tattttcggg taactcgaca tgcaacttat tgttttccca
    15841 ctgcgcaagg gtatagaaca gattgctctc gccacgtaac agccgaaacg tagttttatc
    15901 gaaactgacg ctataaccga tcagcgcgac cattccgtag gcaagcgaaa gcaccagtac
    15961 taccgctgcc gttgccaaca gaaaacgtac ccgcagcgag agcgggaaaa aaagacgcag
    16021 taattttttc atcagcgcaa ttcgaacagg tagccctggc cgcgaacggt ggtaatgact
    16081 tcttgcggat attgtgcctg aatttttttg cgcagacgtc ccatcagtac atcaatggta
    16141 tggctttccc gcagctccgc atccggatag agttgtagca ttaacgaatc tttgctgacc
    16201 actttgccat tattgcgtat caacgtttcc ataatggtgt attcgaacgc ggtcagtttg
    16261 atcacttcgt cattaataga taattcacgg cgagagagat caacctgaaa cggggggagc
    16321 gaaatgacct gtgaagccag accgctatta cgccgcatta atgcctgcat tcgcgccatc
    16381 acctcttcaa tatgaaacgg tttagtcacg taatcatcag caccggcact taatacttcg
    16441 actttgtcct gccagctttc acgggcggtt aataccagaa tcggcagtga aacatcgttg
    16501 ctacgccagc ggcgaatcag tgacagaccg tcctcgtcag gcaatccgag atcgacaatc
    16561 gcaatatccg gtaaatgttc attgagataa taatcggctt ctttggcatc ttctgcgtca
    16621 tcgacctgat gaccagcatc ctgaatctga actttaaggt ggtgacgtaa caacgcattg
    16681 tcttcaacaa ccagtacgcg catttttatt tctccctgtc ttaattatta aaatagtgta
    16741 acgcgattat gtgtttatgg gggtaaacat taaataaacc agcggggagg ggaggtaaag
    16801 tgaaaaaaga aaaagtggat aatcttaata agcaggccgg acagcatcgc catccggcac
    16861 tgatttgagg cttatttcag ctcatcaacc atcgtgatag ctcgaccaat atagttagcc
    16921 ggcgtcatcg ctttcaggcg ggctttctct tcttctggca acgccagacc atcgataaac
    16981 tgcttcatgc cttcggcgtc aacgcgctta ccgcgagtaa gctctttcag cttctcgtac
    17041 ggtttttcga tgccatagcg acgcataact gtctggattg gttctgccag cacttcccag
    17101 ttgtgatcca gctcatccag cagatggtca cggttcactt ccagtttgct cacgcctttc
    17161 agggtggatt gatacgcaat cagcgcataa ccgataccca cgccgaggtt acgcagcacg
    17221 gtggagtcgg tcaggtcacg ctgccagcgg gaaaccggca gtttgcttgc cagatgctgc
    17281 aataccgcgt tggaaaggcc cagattccct tcggagtttt cgaagtcgat cgggttaact
    17341 ttatgcggca tggtggaaga accaatctca ccagcaatgg ttttctgttt gaagtggtta
    17401 agggcgatat aaccccagac gtcacggtca aagtcgatca gaatggtgtt gaagcgcgca
    17461 acgcaatcaa acagttcggc aatgtagtcg tgcggttcaa tctgggtagt gtacgggttc
    17521 cattgaatac ccagcgaggt gacgaactct tcgctgaact gatgccagtc aacttccggg
    17581 taagcggcga tgtgggcgtt atagttaccg accgcgccgt tgattttgcc gaggatctcc
    17641 acctggttaa gctggcggtg ctggcgctcc atacggtagg cgacgtttgc catctcttta
    17701 ccgatggttg acggcgtggc tggctgaccg tgggtacgag acagcagcgg gatatcgcgg
    17761 tactgaacgg cgagatcttt aatgccatca atcagttgac gccagtacgg caggatcact
    17821 tcatcacgcg cggttttcag cattaatgcg tgggagaggt tattgatatc ttccgaagta
    17881 caggcaaagt ggatgaattc agaaaccgcg tgcagttccg ggatctccgc cactttttct
    17941 ttcaggaaat actcaaccgc tttaacgtcg tggttagtgg tacgctcgat ggttttgatg
    18001 cgtgcggcat cttcttcgct gaaattggcg acaattgcat caaggtaacc gattgcgtcg
    18061 gcagcaaaag caggaacttc cttgatcgct gcgtgcgcgg ccagtttttg cagccaacgt
    18121 acttcaactt gtacacggaa tttcagcaaa ccatattcgc tgaaaatccc gcgcagcgcg
    18181 ctgactttat cgccgtagcg tccatcgaca ggggaaacgg cggtcagtga ggataattcc
    18241 atagatcaca actccggggt taaatgagca agaatttgtt ttgcctgagt ggtcaggcga
    18301 ttacgagaaa acatcagttg tagacgtccg ccgccgacct ggtgccagag cacggcggcg
    18361 cgaatgcctg ccagcagggt tgcgcgaact ttcgcctgca cttgtgggct ttgcagtaca
    18421 gcaggggaac cggtgacctg aatgcgcggg ccaagcgggc tgatcacatc aacatagata
    18481 gcagccatcg cgctcatcag cgtttcggac tgtaaatcga agtgttcgag ctggcgttgc
    18541 aggccgttga tccggttgcc cagagtgtcg agcgcgcctt tcgctgagga gagtttgcgc
    18601 tcaagcacca tcaagctgag tgtgtagcgg gttaattcgg cgtttaagcc ctggcaactg
    18661 ctggcattga gcacgccgag cagggtttcc agcccgacgc gcaggttggc ttcgctaccg
    18721 ccaaaaaccg ccagcgtcga gctggggttc atatcaataa tactgttgag tgagacgtgt
    18781 agcgcatcgg catcacaatg cccctggtga gcgagttgtt gcaccaggcg tgccgactga
    18841 caaataccgg ccagggcgag ggtgatgtca tagtaattct ttgccacgtt cactgcttcc
    18901 ttgttttaag taaagataat aatcagaccg gcagcggcag acgctgctca ataataccgc
    18961 caccgaggca cacttcaccg ttatagaaga cggcagactg gcccggcgtc acggcggcaa
    19021 ccggctcatc gaaaatcact ttaatgcgat cgtcgtccag cgccttgacg gtgcacggaa
    19081 tgtcggtctg gcgatagcgg gtttttaccg tgcaacgcat agtgccggtg aatggttcgc
    19141 gatcgaccca gtgcagctgc tgggcaatca acccgacaga catcagccgc gggtgttcat
    19201 ggccctgagc gacaaccaga atgttgtttt cgacgtcttt gtccaccaca taccacggtt
    19261 cttcggtacc ttctttggtg ccaccgatac ccagaccttt acgctgaccg agagtgtgat
    19321 acatcagccc ctggtgctca ccaatttcat cgccatcgac ggtaatgatt ttgcccggtt
    19381 gcgccgggag ataacggccc aggaactcgc ggaatttacg ttcgccaatg aagcaaatgc
    19441 cggtagagtc ttttttcttc gcggtgacca gaccaagatc ttcagcaatc ttacgcacct
    19501 gcggtttttc cagttcgcct accgggaaca ggctttgcgc aatctgctca tggctgagcg
    19561 tataaaggaa gtagctctgg tctttattgc tgtccagacc acgcagcagg cggctcttgc
    19621 catcgacatc ggcccgacgt acgtaatgac cggtagcgat ataatcggca cctaaatctt
    19681 cggcggcaaa ttcgaggaag gctttaaatt tgatctcctt gttgcacaga atatccggat
    19741 tcggcgtgcg accggcttta tattcggcaa ggaacagttc gaagacgttg tcccagtact
    19801 cggcagcaaa gttaacggtg tgcagttcaa tgccgagctt gtcgcagacc gcctgggcat
    19861 cagccagatc cgctgctgct gtgcaatatt cctcaccgtc gtcttcttcc cagttcttca
    19921 taaacaggcc ttcgacctga tatccctgtt gttgcaacag ccaggcagaa acggaggaat
    19981 cgacaccgcc ggacatgccg acgattactt tttttgcggt ttcagacatt ggatcactca
    20041 cgacattgaa cttcaaggcg gcgtattcta tcacgcagcc ttttagatga caccctttgt
    20101 aaaaggccag ttaaaatcgc caatcatctc cagcggataa cgttgcccgc tttgataaca
    20161 acgaatactt tccgccacca gcggcgaacg aagatttgac gcctgtaaaa tttcttcggc
    20221 gctgacccaa cggcagcagt cgatatcgct gtcatgaggc tgggtcgggc atatttgctc
    20281 aagctcaatg gcaaagagga aacgcaaaaa cggcgtttta tctggcgcaa tccactgatg
    20341 catacgaata aagtgttgcg gctgcgcgct gatgccggtt tcttcccaca gttcacgggc
    20401 ggcggcttcc actaaggttt catcggcttc cagatgcccg gcaggttggt tccataacgc
    20461 tttaccatta atcgtctctt caacgactaa aaatttgcct tctgcgtgca ccacgcaagc
    20521 aacggtaacg tgcggtttaa acattttgca tccttaatct gtcacttctc gccattcgcc
    20581 attggcaaga ttatccaaag agtaatctcc catcgcatag cgaatcagtc gcagcgtggg
    20641 gaaaccaaca tgggcggtca tgcggcgcac ctggcgatta cgtccttcat ataaggtgat
    20701 cttcagccag ctggtgggaa tacttttgcg ttcacgaatt ggtggattcc gcggccataa
    20761 ccacgcgggt tcgtcaacca gctccgcgcc tgcgggcagg gtagggccat catttaaggt
    20821 tacgccattg cgcaaggctt caagtgcgtc ttgtgtggga ataccttcca cctgcacata
    20881 atagattttt ccggtgcgtt tacccggctg ggttaaacgc gcctgcaacg cgccgttatt
    20941 ggtcagcacc agcaaccctt cgctatcgcg gtcaaggcga cctgctgcat aaacgccctg
    21001 aaccgggatg aattctttta atgttttgcg tccggtttca tcggtgaact gcggaagaac
    21061 atcgtagggt ttattgaaga ggatcacacg cgtgggctgg ttttcatgtt tacgcctggt
    21121 agaacgttgc gagctgaatc gcttaacctg gtgatttcta aaagaagttt tttgcatggt
    21181 attttcagag attatgaatt gccgcattat agcctaataa cgcgcatctt tcatgacggc
    21241 aaacaatagg gtagtattga caagtcaatt acaaatcatt aacaaaaaat tgctctaaag
    21301 catccgtatc gcaggacgca aacgcatatg caacgtggtg gcagacgagc aaaccagtag
    21361 cgctcgaagg agaggtgaat ggaaagtaaa gtagttgttc cggcacaagg caagaagatc
    21421 accctgcaaa acggcaaact caacgttcct gaaaatccga ttatccctta cattgaaggt
    21481 gatggaatcg gtgtagatgt aaccccagcc atgctgaaag tggtcgacgc tgcagtcgag
    21541 aaagcctata aaggcgagcg taaaatctcc tggatggaaa tttacaccgg tgaaaaatcc
    21601 acacaggttt atggtcagga tgtctggctg cctgctgaaa cccttgatct gattcgtgaa
    21661 tatcgcgttg ccattaaagg cccgctgacc actccggttg gtggcggtat tcgttctctc
    21721 aacgttgccc tgcgtcagga actggatctc tacatctgcc tgcgtccggt acgttactat
    21781 cagggcactc caagcccggt taaacaccct gaactgaccg atatggttat cttccgtgaa
    21841 aactcggaag acatttatgc gggtatcgaa tggaaagctg actctgccga cgccgagaaa
    21901 gtgattaaat tcctgcgtga agagatgggc gtgaagaaaa ttcgcttccc ggaacattgc
    21961 ggtatcggta ttaagccgtg ttctgaagaa ggcaccaaac gtctggttcg tgcagcgatc
    22021 gaatacgcaa ttgctaacga tcgtgactct gtgaccctgg tgcacaaagg caacatcatg
    22081 aagttcaccg aaggcgcgtt taaagactgg ggctaccagc tggcgcgtga agaatttggc
    22141 ggtgaactga tcgacggcgg cccgtggctg aaagttaaaa acccgaatac cggcaaagag
    22201 atcgtcatta aagacgtgat tgctgatgca ttcctgcaac agatcctgct gcgtccggct
    22261 gaatatgatg ttatcgcctg tatgaacctg aacggtgact acatttctga cgctctggca
    22321 gcgcaggttg gcggtatcgg tatcgcccct ggagcaaaca tcggtgacga atgcgccctg
    22381 tttgaagcca cccacggtac tgcgccgaaa tacgccggtc aggacaaagt aaaccctggc
    22441 tctattattc tctccgctga gatgatgtta cgccatatgg gctggactga agccgcagac
    22501 ctgattgtta aaggtatgga aggcgcgatc aacgccaaga ccgtaaccta tgacttcgag
    22561 cgtctgatgg aaggcgctaa actgctgaaa tgttcagagt ttggtgatgc gatcatcaag
    22621 aatatgtaat cactacatgt gttaaatatt taaacgggcg tataacacgc ccgttgtttt
    22681 atttatgtgg atattattaa tagcatatcg agcata
//
LOCUS       DAZPAB010000048        22672 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZPAB010000048 DAZPAB010000000
VERSION     DAZPAB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.048"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            215..1798
                     /locus_tag="ACHUPG_001681"
     CDS             215..1798
                     /locus_tag="ACHUPG_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR0861341.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(2250..4103)
                     /gene="asmA"
                     /locus_tag="ACHUPG_001682"
     CDS             complement(2250..4103)
                     /gene="asmA"
                     /locus_tag="ACHUPG_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR0861342.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4125..4706)
                     /gene="dcd"
                     /locus_tag="ACHUPG_001683"
     CDS             complement(4125..4706)
                     /gene="dcd"
                     /locus_tag="ACHUPG_001683"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR0861343.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4798..5439)
                     /gene="udk"
                     /locus_tag="ACHUPG_001684"
     CDS             complement(4798..5439)
                     /gene="udk"
                     /locus_tag="ACHUPG_001684"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR0861344.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5757..5999
                     /locus_tag="ACHUPG_001685"
                     /pseudo
     CDS             5757..5999
                     /locus_tag="ACHUPG_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            5998..6939
                     /gene="mdtA"
                     /locus_tag="ACHUPG_001686"
                     /pseudo
     CDS             5998..6939
                     /gene="mdtA"
                     /locus_tag="ACHUPG_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            6939..10061
                     /gene="mdtB"
                     /locus_tag="ACHUPG_001687"
                     /pseudo
     CDS             6939..10061
                     /gene="mdtB"
                     /locus_tag="ACHUPG_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            10062..13127
                     /gene="mdtC"
                     /locus_tag="ACHUPG_001688"
     CDS             10062..13127
                     /gene="mdtC"
                     /locus_tag="ACHUPG_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR0861345.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            13128..14543
                     /locus_tag="ACHUPG_001689"
     CDS             13128..14543
                     /locus_tag="ACHUPG_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0861346.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            14540..15943
                     /gene="baeS"
                     /locus_tag="ACHUPG_001690"
     CDS             14540..15943
                     /gene="baeS"
                     /locus_tag="ACHUPG_001690"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR0861347.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            15940..16662
                     /gene="baeR"
                     /locus_tag="ACHUPG_001691"
     CDS             15940..16662
                     /gene="baeR"
                     /locus_tag="ACHUPG_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR0861348.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            16853..17185
                     /locus_tag="ACHUPG_001692"
     CDS             16853..17185
                     /locus_tag="ACHUPG_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR0861349.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            17332..18693
                     /gene="yegQ"
                     /locus_tag="ACHUPG_001693"
     CDS             17332..18693
                     /gene="yegQ"
                     /locus_tag="ACHUPG_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR0861350.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(19035..19352)
                     /gene="yegR"
                     /locus_tag="ACHUPG_001694"
     CDS             complement(19035..19352)
                     /gene="yegR"
                     /locus_tag="ACHUPG_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFR0861351.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            19758..20657
                     /gene="yegS"
                     /locus_tag="ACHUPG_001695"
     CDS             19758..20657
                     /gene="yegS"
                     /locus_tag="ACHUPG_001695"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR0861352.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNSLIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(20739..21512)
                     /locus_tag="ACHUPG_001696"
     CDS             complement(20739..21512)
                     /locus_tag="ACHUPG_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR0861353.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(21618..22658)
                     /gene="gatD"
                     /locus_tag="ACHUPG_001697"
     CDS             complement(21618..22658)
                     /gene="gatD"
                     /locus_tag="ACHUPG_001697"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR0861354.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         5234 a         6045 c         5906 g         5487 t
ORIGIN      
        1 tttctttagg ttgacaatat ttaatattgc gtctccacat gcgatatttc ttaaataatg
       61 ttttattatt accgctttta attcagggat aatgtgaggt tattaccctc aaataatatg
      121 agataaatag tgctgcaaca ttgcattttt gccccgattt atccatgatc gaattgtgac
      181 atttgtcata caacgaatag gttttgtact tactatggaa tggattgccg atccgtctat
      241 ctgggccggg ttaatcacgc tgattgtgat cgaactggtc ctcggcattg ataacctggt
      301 ctttattgcc atcctcgccg aaaaactacc gccgaagcag cgcgaccgcg cacgggttac
      361 cgggctgctg ctggcgatgt taatgcgcct gttactgcta gcgtcaatct cctggctggt
      421 caccctgact caaccgctgt tcagcttccg ctcgtttacc tttagcgccc gcgacttaat
      481 catgctgttt ggtggtttct tcctgttgtt caaagccacg atggagctga acgaacggct
      541 ggaagggaaa gacagcaata atcccacaca acgcaaaggt gcgaagttct ggggcgtggt
      601 gacgcaaatt gtggtactgg acgccatttt ctcactcgac tcagtgatta ccgccgtcgg
      661 gatggtcgac catttactgg tcatgatggc cgccgtggtt atcgctatca gcctgatgtt
      721 gatggccagc aagccgttaa cgcaatttgt taacagtcac ccgaccatcg ttattctctg
      781 cttaagcttc ctgttgatga ttggctttag cctggtggca gaaggtttcg gcttcgtcat
      841 tccgaaaggc tacctgtacg ctgccattgg tttctcggtg atgatcgagg cactcaatca
      901 gttggctatc tttaaccggc gacgttttct ttccgctaat cagacgctac gccagcggac
      961 taccgaagcg gtaatgcgcc tgcttagcgg gcaaaaagaa gatgcggaac tggacgccaa
     1021 aaccgcgtcc atgctggtgg atcatggaaa ccagcagatc tttaatccgc aggaacggcg
     1081 gatgattgag cgggtactta atcttaacca gcgtaccgta agcagcatta tgacgtcgcg
     1141 ccacgatatt gagcatatcg atctcaacgc gccggaagaa gaaatccgcc agttgctgga
     1201 gcgaaatcag catacgcggc tggtggttac cgacggtgat gacgcagaag atttgctcgg
     1261 tgttgttcac gttatcgacc tgctacaaca gtcactgcgc ggcgaaccgc tcaacctgcg
     1321 ggtgttgatt cgccagccgc tggtgttccc ggaaaccttg ccgttgttac cagccctgga
     1381 gcagttccgt aatgcccgca cgcactttgc ttttgtggtg gatgagtttg gctcggtgga
     1441 agggattgtg acattaagtg acgtcactga aaccattgcc ggtaacttac cgaacgaagt
     1501 ggaagagatc gacgcccgcc atgatattca gaagaatgcg gacggttcct ggacggcgaa
     1561 tggtcatatg ccgctggagg atctggtgca atatgtgccg ctgccgctgg atgaaaaacg
     1621 tgaatatcac accattgccg ggctgttgat ggagtatttg cagcgtattc caaaacctgg
     1681 cgaagaagtt caggtggggg attatttgct taaaacgttg caggtggaaa gccatcgcgt
     1741 gcagaaggtg cagattatac cgctgcgtaa ggatggcgag atggagtacg aggtgtgatg
     1801 gtatcggcca ttttacccaa tgctgcgtaa cgccttataa cttcagcaat gaagcgttgt
     1861 ctgtggtaaa cggacatcac agcgggggtg tgggtccggc tgtaaatcgc cgaatcgttt
     1921 cctgtaggct ggggcagccc acatggatgt gggctgaggg cgcgttttac agggatgtta
     1981 cctcgcgccc gtacccgata gccgaaagga ataagatgag ggaaccgcgc cagcggcgat
     2041 ttaccgccgg gagcccgggt tgccagggtg gtggcgattg agccaccctg gcacgttcac
     2101 cggcttgagc gttccatagt agcgaggaac atcaagtgaa cggaacagcc ttcgatctta
     2161 catattcaga atataatagt gccgccgcga acgcctgatg cgacgcttta cgcgtcttat
     2221 caggcctaca ggacaggtca cctggtaacc tacatcttct ccagcaactt cttcacatct
     2281 ttgccattgc gggaatcttt attccgctcg gcccagtcat tcaggcgacg tttcgcttcg
     2341 tcctgtagat gtttgcgcag taactgatcc acttgcaggc tgtaattgag ttgctgccag
     2401 ttgccataaa cccgcagcgg tactggcgtt tctttgagga aatcaatcag tttgctttcc
     2461 ccgttccagc cacccacgac ccgaatatca aactgggtgt cgcaggtttg atctgccaga
     2521 ttcaacatgc cttcccctgt cagcgccagc acaggcgatt gaccttgcat gtcgtttaac
     2581 gtcacgacgc catccttcaa cgtcaaatcg gtggtaaagc ggtcaagccg cgtcacgtta
     2641 tcgaagtttt cagcggcctt cacatcaccg ccattacgct ctaccgcttg ctgaatcatc
     2701 tgctggaagt tcatcccttc catacgtgtg tcggtcatct caacatgcgc ctgtccttgc
     2761 cagttgtggc ggaatgcgtc ggcatctatg tcagcaccgg agaaatcacc agccagtgac
     2821 atttttccgg tcaacgaaat cggatagtta aacgccttca gaatggtacc aatctcaacg
     2881 ttctccagcc gtggctggaa gtttatccgc ggatttattg atgtcgcgtc cagcgtgccc
     2941 ggcagtgaaa cctttccacc gttaagcttg ccctgcagtt gagtaatttc cagcaaaccc
     3001 gacttgttgg tcatttgcgt ggcaacatct gtaaaattca ttccgcgcca gcgcacgtta
     3061 ctggcctgca acaaaatatc agctgtaaag ccttgcagtc cctgataggc cggttcatca
     3121 atacgcgaag aaatgaccgg gcgcggcaac gttgattggc tctgcccctg ctgcgcggca
     3181 ccgttttcac cattcgcggt ttcattaagc gggatgaggt tgtcaagatt cagttgcggg
     3241 aattgcagtc tcagctgcca ttccggtttc tctgtcagcg tgacctgtgc ttgtccgctc
     3301 agcgtactgt cattggcggt caaactaatt tggttaaatg aaaggcgttt atgtgactcc
     3361 tgccactggg actggaaact cccctgcccc tgaatacctt gttttggtaa atcggcaccc
     3421 tgcaactgcc agttaatttg ttcaatagcc gctgttaaat catgcggata atcagaagca
     3481 tctaccgtac cgttaaggga tatcgtgaga tcgcgctgat cgcgattaac ccgcccggag
     3541 aactcaaatg agccacgatg ttggggatct tgttccattt gcaggcggat attgcggatt
     3601 gtcacctgct cgtcatcttc atgctggaac accagaacgc tgtccgccac cttaagactg
     3661 gatatatcaa acgaccatcc gcgatcgtct gacagatccg gcaaggtatt gtcgcgcggt
     3721 gcaaccggag cgtcttcact gcgcaccgct tccgtctgcg gcgtcagttg gatcactgcc
     3781 ccttttagca tcacctgctt aacgctcagt tgatgactca gtagtggtaa aagcgccacg
     3841 tccagacgca tgttgtcggc gcgaaccagt ggctggcttg cgccctgggc ggtgagagac
     3901 attcgcccgg agaggatact aagctgcggc cagacgtgcc aacgcagtgg cccgtcgagc
     3961 tgcaattgat aaccgctacg tgcagcaact tgcttgacca tatagtcgcg gaaatcattc
     4021 ggattcacca gcaacactaa cgcagataac ccggccacca gcacgaccaa gagtatcatc
     4081 agcgtcgtca gaaatcgtct catggtatcc tcaatgggcc tgaattagtc tttatcgatt
     4141 cggctggcta ccgcgccctg ctggttgcga tatttcgcat cttcacggcg gttgtaaggt
     4201 cgcgccgccg ggccggaaag cggctcaaag ctcagcgcac caattaacat gcccggacgc
     4261 agcgccagcg gcagcttacc ggagttgtag aactccagca caatgcaacc agaccagccc
     4321 ggatcgatac ggtgcgcggt gacgtgcacc atcagcccca gacgcgccaa agaggaacgc
     4381 ccgtccagcc agcccaccag atcggctggc agcgtcaccg actccagtgt caccgccagc
     4441 gccagctctc ctgggtgaag atagaacgcc tcgccctcgt cgagaacgat ctcatcgctc
     4501 atcacgcggt caagcgcggc gctcacttca tctttgggac cgctcagatc gataaacgct
     4561 gccgtgtgac cacggaaggt acgaaattta ttgcccaggc gtacatccac cgtcgcgccg
     4621 ttaatacgct ccactggcgg acgtgggttg atcgacaaac ggccttcatc aagccaggct
     4681 tcaatatctc ggtcacacag acgcatggca ttttctcctt tcgcacatca ctcccttaac
     4741 gccaattacg tcaagggcat accaacttat cactgaacgg tacacaattt atcaagctta
     4801 ttcaaagaac tgacttattt tcgctttcaa tatatcgatc gcgatgcggt ttttcccgcc
     4861 gcgcggcacg ataatgtccg catattgttt agaaggctca atgaattgca ggaacatcgg
     4921 gcgcacggtt ttttgatatt gcgccatcac ggaatccatt gaacgcccac gctcgttaac
     4981 gtcacgcttg atgcggcgca tcaggcagat atccagcggg gtatcaacga aaatggagaa
     5041 gttaagttcg tcacgcaaac gcgcatccgt cagcaacaaa atgccttcga gaatgatgac
     5101 cttcttcggc tcaaccgtca ccgtttcttt catacgcgta tgttcaacat agctgtaaac
     5161 cggcaggtca attgccgagc cgcgtttcaa cgcttgtaaa tgctcaagca acaggctgtg
     5221 atccatcgcg ctgggatggt cgtagttggt cttaacgcgt tcttccatcg acagatggct
     5281 ttgatctttg taatagcagt cttcgggaat tacgccgatg tgttcatcac cgacttgctc
     5341 acgtaattca cgataaaggg tactggcaat aagactcttg ccggaagccg atgcgccagc
     5401 gataccgata atgacgcact gatgagactg atcagtcata tatttagcga cctgattaac
     5461 ctggatgtta ggaagggggc gacgaagcgc caaacgcggc aattataggg atttcatccg
     5521 tctgatacca gtcgaatagc gttgccgcgc gctcagagtt aattgttgac aaagaattcc
     5581 cggtggcaaa ttacgttgat cagttttatg taaggtaaaa aatgttatac gcagttgcgc
     5641 aaattatccg cctttacgtc actttatgag caattcgcat ataaaatgta aaacttttgt
     5701 actagcataa acacagaaac gaatactggc gacctggcct tgcggataaa gcggtaataa
     5761 gcaaacaatc acagcatgta ttaattgccc tgccccaccc gctgcttcac ctggtcagtt
     5821 taggtttagt ctcgtttatc tttacccttg tctcgcttga gctttcgcag tttggcaccc
     5881 aactcgcccc actgtggttc ccgacgtcca tcatgatggt ggcgttttat cgccatgccg
     5941 ggcgcatgtg gccgggaatt gcgctgagct gttcgctggg aaatatcgcc gcatccattc
     6001 caggaaggcc agcaggtcaa agcaggcgat ttactggcag aaattgaccc cagccagttc
     6061 aaagttgcat tagcacaaac ccagggccaa ctggcaaaag ataaagccac gcttgctaac
     6121 gcccgccgtg atctggcgcg ttatcagcaa ctggcaaaaa ccaatctcgt ctcccgtcag
     6181 gagctggatg cccaacaggc gctggtcagt gaaaccgaag gcaccattaa ggctgatgaa
     6241 gcaagcgtcg ccagcgcgca gttgcaactc gactggagcc gcatcaccgc accagtcgat
     6301 ggtcgcgttg gtctcaagca agttgatgtt ggtaaccaaa tctccagtgg tgataccacc
     6361 ggaattgtgg tgatcaccca gacgcatcct atcgatttag tctttaccct gccggaaagc
     6421 gatatcgcta ctgtagtgca ggcgcaaaaa gccggaaaac cgctgatggt agaagcctgg
     6481 gatcgcacca attcgaagaa attaagtgaa ggcacgctgt taagcctcga taaccaaatc
     6541 gatgccacaa ccggtacgat taaagtgaaa gcacgcttta ataatcagga tgatgcgctg
     6601 tttcccaatc agtttgttaa cgcgcgcatg ttagtcgaca ccgaacaaaa cgccgtagtg
     6661 atcccaacag ccgccctgca aatgggcaat gaaggccatt ttgtctgggt gctgaatagc
     6721 gaaaacaagg tcagcaaaca tctggtgacg ccgggcattc aggacagtca gaaagtggtg
     6781 atccgtgcag gtatttctgc gggcgatcgc gtggtgacag acggcattga tcgcctgacc
     6841 gaaggggcga aagtggaagt ggtggaagcc cagagcgcca ccactccgga agagaaagcc
     6901 accagccgcg aatacgcgaa aaaaggagca cgctcctgat gcaggtgtta cccccgagca
     6961 gcacaggcgg cccgtcgcgc ctgtttatta tgcgtcctgt cgccaccacg ctgctgatgg
     7021 tggcgatctt actcgccggg attatcggtt atcgcgccct gcccgtttcg gcgctgccgg
     7081 aagtggacta tccgaccatt caggtggtca cgctctaccc aggtgccagc ccggatgtca
     7141 tgacctctgc cgttaccgcg ccgctcgaac gccagttcgg gcagatgtct ggcctgaaac
     7201 agatgtcgtc gcaaagttcc ggcggtgcgt cagttatcac tttgcagttc cagctaacat
     7261 taccgctcaa tgtcgccgag caggaagtgc aggccgcgat taacgctgcg accaacttgt
     7321 tgccgagcga tctgcctaac ccgccggttt acagcaaagt gaacccggca gatccgccga
     7381 tcatgacgct cgccgtcacc tcaaccgcca tgccgatgac gcaagtggaa gatatggtgg
     7441 aaacccgcgt cgcgcagaaa atctcgcaga tttccggcgt cggcctggtg acgctttccg
     7501 gcggtcagcg tccggctgtt cgcgtcaaac ttaacgctca ggcgattgcc gccctcggcc
     7561 tgaccagcga aaccgtgcgc accgccatta ccggcgctaa cgttaactcg gcaaaaggta
     7621 gcctcgacgg cccttcccgt gcggtcacgc tttccgcgaa cgaccagatg caatccgccg
     7681 aagagtatcg ccagctaatc atcgcctacc agaacggcgc gccaattcgt ctgggcgatg
     7741 tcgcaactgt agagcaaggt gcagaaaaca gctggctcgg cgcgtgggcg aacaaagaac
     7801 aggccattgt gatgaatgtt cagcgccagc ccggtgctaa cattatctcc accgccgaca
     7861 gcattcggca gatgctgcca cagctcactg agagtctgcc gaaatcggtg aaggtgacag
     7921 tgctttccga tcgcaccacc aatatccgcg catccgtcga tgatactcag tttgaattga
     7981 tgatggctat cgcgctggta gtcatgatta tctacctgtt tttgcgcaat attccggcga
     8041 ccatcattcc cggtgttgct gtaccgctgt cgttaatcgg cactttcgcg gttatggtgt
     8101 ttctcgattt ttcaatcaat aacctgacac tgatggcttt aactatcgcc accggattcg
     8161 tggtcgatga cgccatcgtg gtgatcgaaa acatttcccg ctatatcgaa aaaggcgaaa
     8221 aaccgttggc ggcggcgctc aagggcgcag gtgaaatcgg ctttaccatt atctcgctga
     8281 ccttctcact gattgcggtg ttgatcccac tgctgtttat gggcgatatc gtcgggcgac
     8341 tgttccgcga atttgctatt accctggcgg tagcgatttt gatctcagcg gtggtgtcgc
     8401 tgaccctgac accgatgatg tgcgcgcgga tgctcagcca ggagtcgttg cgtaaacaga
     8461 acagcttctc ccgtgcctcg gaaaaaatgt tcgacaggat aatcgccgcc tatggtcgtg
     8521 gactggcgaa agtgctgaat catccgtggc tgaccttaag cgtggcactc agcacgctgc
     8581 tgcttagcgt gctgctgtgg gtgttcattc cgaaaggttt cttcccggta caggacaacg
     8641 gcattattca gggcactttg caggcaccgc aatccagctc ctttgccaat atggcccagc
     8701 gacaacgcca ggtcgcggac gtgattttgc aggatccggc agtgcaaagc ctgacctcat
     8761 ttgttggcgt tgatggcact aacccgtcgc tgaatagtgc acgtttacaa atcaacctca
     8821 aaccgttgga tgaacgtgat gatcgggtgc aaaaagtcat cgcccgtctg caaacggcgg
     8881 tagataaagt gccgggcgtc gatctcttcc tgcaaccaac gcaggacctg accatcgata
     8941 ctcaggtcag ccgcacccag taccagttta ccttgcaggc cacgtcactg gatgcgctca
     9001 gtacctgggt gccacagttg atggaaaaac tccagcaact gccacagctt tctgatgtct
     9061 ccagcgactg gcaggacaaa gggctggtgg cgtatgtcaa tgttgatcgc gacagcgcca
     9121 gccgtctggg gatcagcatg gcggacgtcg ataacgccct gtacaacgcg tttggtcagc
     9181 gactgatttc cactatttat actcaggcta accagtatcg cgtggtgctg gagcacaata
     9241 ccgaaaatac cccaggcctc gcggcgctgg ataccattcg cctgaccagc agcgacggtg
     9301 gcgtggtgcc gttaagctca attgccaaaa ttgagcagcg ttttgcgccg ctttccatca
     9361 accatctgga tcagttcccg gtaacaacca tctcctttaa cgtgccggat aactattcgc
     9421 tgggcgatgc ggtgcaggcg attatggaca ccgaaaagac gctgaatctg ccggtggata
     9481 tcaccacgca attctagggc agcaccctcg ccttccagtc ggcgctgggc agcactgtct
     9541 ggctgattgt cgcggcggtg gtggcgatgt atatcgtgct cggcattctg tacgagagct
     9601 ttattcaccc gatcaccatt ctctcgacgc tacccaccgc aggggtcggc gcgctgctgg
     9661 cattaatgat tgccggtagc gaactggatg tgattgcgat tatcggcatt attttgctga
     9721 tcggtattgt gaagaagaac gccatcatga tgatcgactt cgcgctggct gctgagcgcg
     9781 agcaaggcat gtcgccgcgc gatgcaatct accaggcttg tctgttgcgt tttcgtccga
     9841 tcctgatgac caccctggcg gctctgcttg gcgcgctgcc gctgatgttg agtaccgggg
     9901 tcggcgcgga actgcgtcgt ccgttaggta tcggcatggt cggcggtctg attgtcagcc
     9961 aggtgctgac gctgttcacc acgccggtga tttatctgct gttcgaccgc ctggcattgt
    10021 ggaccaaaag tcgctttgct cgccatgaag aggaggcgta agtgaagttt tttgccctct
    10081 tcatttaccg cccggtggcg acgattttac tgtcggtcgc cattaccctg tgcggcatac
    10141 tgggtttccg tatgctgccg gtcgccccgc tgccgcaggt cgattttccg gtgattatgg
    10201 tcagcgcctc gctgcccggt gcgtcgccgg agacaatggc gtcttccgtt gccacgccgc
    10261 tggagcgctc acttgggcgc attgccgggg ttagtgaaat gacctccagc agttcgctcg
    10321 gcagcacgcg cattattttg cagtttgatt ttgaccggga tatcaacggc gcagcgcgtg
    10381 atgtgcaggc ggcgatcaac gctgcacaaa gtttgctgcc cagcgggatg cccagccgcc
    10441 cgacctatcg caaagcgaac ccgtcggatg cgccaattat gatcctcacg ctgacatccg
    10501 atacttattc gcagggtgaa ctgtacgatt tcgcctcgac gcagctggct ccgacgattt
    10561 cgcaaatcga cggtgtcggt gatgtcgatg tcggtggcag ctcgctgccc gccgtgcgcg
    10621 tcgggctgac cccgcaggcg ctgtttaatc agggcgtgtc gctggacgac gtacgcactg
    10681 ccatcagcaa tgccaacgtg cgtaaaccgc agggggcgct ggaagatggc actcaccgct
    10741 ggcagatcca gaccaacgat gaactgaaaa ccgccgctga atatcagccg ctgattattc
    10801 actacaacaa cggcggcgcg gttcgtctgg gcgatgtggc gacggtgact gactcagtgc
    10861 aggatgtgcg caacgccggg atgaccaacg ccaaaccggc tattttactg atgatccgca
    10921 aactgccgga agccaatatt atccagacgg tagacagcat ccgggcaaaa ttaccagagt
    10981 tgcaggagac cattccggcg gcgattgatc tgcaaattgc ccaggatcgc tcccccacca
    11041 ttcgcgcctc gctggaagaa gtcgagcaaa cgctgattat ctcggtggcg ctggtgattc
    11101 tggtggtctt tttattcctg cgctcgggtc gcgccactat tattcccgcc gttgcggtgc
    11161 cggtttcgct gattggtacg tttgcggcga tgtacctgtg cggattcagt ctcaataatc
    11221 tttcgctgat ggcgctcacc attgctactg gtttcgtggt ggatgacgcc attgtggtgc
    11281 tggaaaacat tgcccgtcat ctggaagcgg ggatgaaacc actacaggcc gcgctgcaag
    11341 gtactcgcga agtcggcttt acggtgctgt cgatgagtct gtcactggtg gcggtgttcc
    11401 tgccgctgct gttgatgggc ggattgccgg gccgactgtt acgcgagttt gccgtgacgc
    11461 tttctgtcgc cattggtatt tcgttgctgg tttccctgac gctaacgcca atgatgtgcg
    11521 gctggatgct aaaagccagc aagccgcgtg agcaaaagcg actgcgtggt tttggccgca
    11581 tgttggtagc cctgcaacaa ggctacggca agtcgctgaa atgggtgctc aatcataccc
    11641 gtctggtggg cgtggtgctg cttggcacca ttgcgctgaa tatctcgatc ccgaaaacct
    11701 tcttcccgga gcaggacacc ggcgtactga tgggtgggat acaggcggat cagagtattt
    11761 cgtttcaggc gatgcgcggc aaattgcagg atttcatgaa aattatccgt gacgatccgg
    11821 cagtggataa tgtcaccggc tttaccggcg gttcacgagt taacagtggg atgatgttta
    11881 tcaccctcaa gccacgcgac gaacgcagcg aaacggcgca gcaaattatc gaccgtctgc
    11941 gcgtgaagct ggcgaaagaa ccgggggcga atctgttcct gatggcggta caagatattc
    12001 gcgttggtgg gcgtcagtcg aacgccagct accagtacac gttgttatcc gacgacctgg
    12061 cggcactgcg cgaatgggag ccgaaaatcc gcaaaaaact ggcgacgttg ccggaactgg
    12121 cggacgtgaa ctccgatcag caggataacg gcgcggagat gaatctggtt tacgaccgcg
    12181 acaccatggc acggctggga atcgacgtac aggccgccaa cagtctgtta aataacgcct
    12241 tcggtcagcg gcaaatctcg accatttacc agccgatgaa ccagtataaa gtggtgatgg
    12301 aagtggatcc gcgctatacc caggacatca gtgcgctgga aaaaatgttc gttatcaata
    12361 acgaaggcaa agcgatcccg ctgtcgtatt tcgctaaatg gcaaccggcg aatgccccac
    12421 tatcggtgaa tcatcaggga ttatcggcgg cctcgaccat ctcgttcaac ctgccgaccg
    12481 gaaaatcgct ctcggacgcc agtgcggcga tcgatcgcgc aatgacccag cttggtgtgc
    12541 cttcgacggt gcgcggcagt tttgccggca cggcgcaggt tttccaggag acgatgaact
    12601 cgcaggtgat cctgattatc gccgccatcg ccacggtgta tatcgtgctg ggtatccttt
    12661 acgagagtta cgtacatccg ctgacgattc tctccaccct gccctcggcg ggcgttggag
    12721 cgctgttggc gctggagctg ttcaatgccc cgttcagcct aatcgccctg atagggatca
    12781 tgctattaat cggcatcgtg aagaaaaacg ccattatgat ggtcgatttt gcgcttgaag
    12841 cccaacggca cggtaacctg acgccgcagg aagctatttt ccaggcctgt ctgctgcgtt
    12901 ttcgcccgat tatgatgacc accctggcgg cgctgtttgg cgcgctgccg ctggtattgt
    12961 cgggcggtga cggctcggag ctgcggcaac ccctggagat caccattgtc ggcggtctgg
    13021 tgatgagcca gctcctgacg ctgtatacca cgccggtggt gtatctcttt ttcgaccgtc
    13081 tgcggctgcg tttttcgcgt aaacctaaac aaacggtaac cgagtaaatg acagatcttc
    13141 ccgacagcac ccgctggcaa ttatggattg tggctttcgg cttctttatg cagtcgctgg
    13201 acaccaccat cgtaaacacc gcccttccct caatggcgca aagcctcggg gaaagtccgt
    13261 tgcatatgca catggtcatt gtctcttatg tgctgaccgt ggcggtgatg ctgcccgcca
    13321 gcggctggct ggcggacaaa gtcggcgtgc gcaatatttt ctttaccgcc atcgtgctgt
    13381 ttactctcgg ttcactgttt tgcgcgcttt ccggcacgct gaacgaactg ttgctggcac
    13441 gcgcgttaca gggcgttggc ggtgcgatga tggtgccggt cggcaggttg acggtgatga
    13501 aaatcgtacc gcgcgagcaa tatatggcgg cgatgacctt tgtcacgtta cccggtcagg
    13561 tcggtccgct gctcggtccg gcgctcggcg gtctgctggt agaatacgcg tcgtggcact
    13621 ggatcttttt gatcaacatt ccggtgggga ttatcggtgc gatcgccaca ttgatgttaa
    13681 tgccgaacta caccatgcag acgcggcgct ttgatctttc cggcttttta ttgctggcgg
    13741 ttggcatggc ggtattaacc ctggcgctgg acggcagtaa aggtacaggt ttatcgccgc
    13801 tggcgattgc tggcctggtc gcagttggcg tggtggcact ggtgctttat ctgctgcacg
    13861 ccagaaataa caaccgcgcc ctgttcagtc tgaaactgtt ccgcactcgt accttttcgc
    13921 tgggcctggc ggggagcttt gccggacgta ttggcagtgg catgttgccc tttatgacac
    13981 cggttttcct gcaaattggc ctcggtttct cgccgtttca tgccggactg atgatgatcc
    14041 cgatggtgct tggcagcatg ggaatgaagc gaattgtggt acaggtggtg aatcgctttg
    14101 gttatcgtcg ggtactggta gcgaccacgc tgggtctgtc gctggtcacc ctgttgttta
    14161 tgactaccgc tctgttgggc tggtactacg ttttgccgtt cgtcctgttt ttacaaggga
    14221 tggtcaactc gacccgtttc tcctccatga acaccctgac gctgaaagat ctcccggaca
    14281 atctggcgag cagcggaaac agcctgctgt cgatgattat gcaattgtcg atgagtatcg
    14341 gcgtcaccat cgccgggctg ttgctggggc tttttggttc gcagcatatc agcgtcgaca
    14401 gcggcaccac acaaaccgtc tttatgtaca cctggcttag catggcgttt atcatcgccc
    14461 ttccggcgtt catctttgcc agagtgccga acgatacgca tcaaaatgta gctatttcgc
    14521 ggcgaaaaag gagcgcgcaa tgaagttctg gcgacccggt attaccggca aactgtttct
    14581 ggcgattttc gccacctgca ttgtcttgct gatcagtatg cactgggcgg tgcgtatcag
    14641 ttttgagcgt ggctttattg attacatcaa acacggcaat gaacagcgat tacagctgtt
    14701 aagtgatgcg cttggcgagc agtatgcgca gcatggtaac tggcgcttcc tgcgcaacaa
    14761 cgatcgcttt gtctttcaga tcctgcgttc atttgaacac gataattcgg aagataaacc
    14821 cggcccgggt atgccgccgc acggctggcg tacccagttc tgggtggtcg atcaaaacaa
    14881 caaagtactg gttggtccgc gagcgccgat tccacctgac ggtacacggc gacccattct
    14941 ggtcaacggt gcggaagtgg gcgcggtgat tgcctcccct gttgagcggc tgacccgcaa
    15001 tactgatatc aatttcgata aacaacagcg gcaaaccagc tggctgatcg tcgccctggc
    15061 aacgttactc gcggcgctcg ccacctttct gctggcgcgc ggtttgctgg caccggtaaa
    15121 acgacttgtc gatggcacgc acaaactggc ggcgggcgat ttcactaccc gcgtaacgcc
    15181 caccagtgaa gatgaactgg gcaaactggc gcaagacttc aaccagctcg ccagcacgct
    15241 ggagaaaaac cagcaaatgc ggcgcgattt tatggccgat atttctcacg aactgcgtac
    15301 gccattagcg gtgctgcgcg gtgaactgga agccattcag gatggcgtgc gcaaattcac
    15361 gccggagacg gtggcgtctt tacaggcgga ggtcggtaca ctgaccaaac tggttgacga
    15421 tcttcatcag ttgtcgatgt ctgatgaagg cgctctcgcc tatcaaaaag caccggtaga
    15481 tttgatccca ctgctggaag tggcgggcgg cgcatttcgc gaacgattcg ccagccgtgg
    15541 cctgaaactg caattttccc tgccagacag tatcaccgta tttggcgatc gcgaccgttt
    15601 aatgcagtta ttcaataact tactggaaaa cagcctgcgc tacactgaca gcggcggcag
    15661 cctgaaaatc tctgccgagc agcacgacaa aacggtgcgc ctgacctttg ccgacagtgc
    15721 gccaggtgtc agtgacgatc agctacaaaa attgtttgaa cgtttttatc gcaccgaagg
    15781 ttcccgcaac cgcgccagcg gcggttccgg gctggggctg gcgatttgcc tgaacattgt
    15841 tgaagcccat aatggccgca ttattgccgc ccattcgcct tttggcgggg taagcattac
    15901 agtagagtta ccgctggaac gggatttaca gagagaagta tgaccgagtt accaatcgac
    15961 gaaaacacac cgcgtatttt gatcgtggaa gatgaaccga agctggggca gttgctcatt
    16021 gattatctgc gtgctgcgag ctatgcgccg acgcttatca gccacggcga tcaggtactg
    16081 gcgtatgtgc gccagacacc accggatctg atcctgttgg atctgatgct ccctggcacc
    16141 gatggcctga cattatgccg ggaaattcgt cgtttttccg atgttccgat cgtgatggtg
    16201 acggcaaaaa tcgaagagat cgaccgcctg ctggggctgg agattggcgc cgatgactac
    16261 atctgcaagc cgtacagccc acgggaagtg gtagcgcgtg tcaaaaccat tttgcgccgt
    16321 tgtaaaccgc aacgcgaact tcagcaacag gatgccgaaa gcccgttgat tatcgacgaa
    16381 ggtcgttttc aggcttcatg gcgcggtaaa atgcttgacc tgacgcctgc ggaatttcgt
    16441 ctgcttaaaa cgctctctca cgaaccagga aaagtgttct cccgcgagca attgctcaat
    16501 catctttatg acgactaccg cgtagtaacc gaccgcacca ttgacagcca cattaaaaat
    16561 ctgcgccgca agctggaatc tctcgacgcc gaacagtcat ttatccgcgc cgtttacggc
    16621 gttggttacc gctgggaagc cgacgcctgc cgcatcgttt agttttagcg acattacttt
    16681 gttagccgga gatggcgttc agacccgatc cggcaacatt attccacgca tggtcagcaa
    16741 actgacatta tctctccgcc ctggcaggca tcagaaaatc gctcatactt taatcagtaa
    16801 acagcacctt tagatgctgt tttcgataag caatttcaat caaggagtca ttatggctgg
    16861 ttggtttgaa ctcagtaaga gcagtgataa tcagttccgg tttgtgctaa aagcgggcaa
    16921 tggtgagact atcctcacca gcgagctgta tacctcaaaa gcctctgcgg aaaagggcat
    16981 cgcgtcggtg cgtagcaaca gcccgcaaga agagcgctat gagaaaaaaa cggcaagcaa
    17041 cggcaaattc tatttcaatc tgaaagccgc taatcatcaa attatcggct ccagccagat
    17101 gtacgccacc gcgcaatctc gtgaaaccgg aattgcctcc gttaaagcca atggcacaag
    17161 ccagacggtg aaagacaata cgtaattacg atgccgggcg tgggttgttg gcgtccggcg
    17221 ctttaccaga tcccccctac agcgctacaa tgcccgccct taaagtgggg gcctccccta
    17281 accgcttcat caggtgaagc ggatctgacc tgtcatcaga acgagagaat tatgtttaaa
    17341 ccggaactcc tttccccggc gggaacgctg aaaaatatgc gttacgcttt cgcttatggc
    17401 gcagatgctg tttatgcggg ccagccgcgt tactccctac gtgtgcgcaa caacgaattc
    17461 aaccacgaaa atcttcagct cggcatcaat gaagcccacg cgctggggaa aaagttttat
    17521 gtcgtggtca acattgcacc gcacaacgcc aagctgaaaa cctttatccg tgacctgaaa
    17581 ccggtggtgg aaatggggcc ggatgcgctg attatgtccg atccagggct gattatgctg
    17641 gtgcgtgagc acttccctga aatgccgatc cacctctcgg tgcaggctaa cgccgtgaac
    17701 tgggcgacgg tgaaattctg gcagcaaatg ggactgaccc gcgtgatcct ctctcgcgag
    17761 ctgtcgctgg aagagattga agagatccat aatcaggtgc cggatatgga gatcgagatc
    17821 ttcgttcacg gcgcactgtg catggcctac tccggtcgct gcctgctctc tggctatatc
    17881 aacaagcgcg acccgaacca gggcacctgc accaacgcct gccgctggga gtacaacgtc
    17941 caggaaggga aagaagatga cgttggcaac atcgtacaca agtacgagcc gattccggtg
    18001 caaaatgttg agccgacgct gggtatcggc gcgccaaccg acaaagtgtt tatgatcgaa
    18061 gaagcccagc gtccgggcga gtatatgacc gcgtttgaag atgagcacgg cacttacatc
    18121 atgaactcga aagatctgcg cgccatcgcc catgtagaac gcctgaccaa aatgggcgtg
    18181 cattcgctga aaatcgaagg tcgtaccaaa tctttctact attgcgcacg caccgcacag
    18241 gtttaccgca aagctatcga tgacgccgct gcgggaaaac cgttcgatac cagcctgctg
    18301 gaaactctgg aaggtctggc gcatcgtggc tataccgaag gtttcctgcg tcgtcatact
    18361 cacgacgatt atcagaacta cgaatacggt tattcagttt ctgaccgcca gcagtttgtt
    18421 ggtgagttta ccggtgagcg caagggggac ctcgcagcgg tagcggtgaa aaataaattc
    18481 tccgttggcg acagtcttga gctgatgacg ccgcaaggca acattaactt tacccttgag
    18541 cacatggaaa acgccaaagg cgaagctatg ccggtcgcac caggcgatgg ttatactgtg
    18601 tggctcccgg tcccgcagga tcttgagcta aattacgcgc tgctaatgcg taatttctcc
    18661 ggggaaacca cgcgtaaccc ccacggtaag tgattaattt cgattatttt tcccggatgg
    18721 aaaattctta gaaaccgatc acatacagct gcatttatta aggttatcat ccgtttcgct
    18781 gaaaaacata atccataaaa tgctagctgt accaggaacc acctccttag cctgtgtaat
    18841 ctcccttaca cgggcttatt ttttgtacat aagctgtaaa gaaggattga tccctgataa
    18901 ccgtcttcca ttgaccacat cgatagaatc ccccttcata gcacaatgcc tttcacgtaa
    18961 cggtatcgtg ctcgcccagg ttccggctac gcacagccag aacgcgcatg tttgacgctt
    19021 acaaaaaaat attctcactc tccacatttg aatgtcagac gagcgacgcc atgtaatcct
    19081 gcaccttctg tcttcaggtc aactatctgc atttttttgc cctgagtaac acagaaatgg
    19141 gctgcatcat tttttactat attttctgca ccagatattc tgcccctggc taaagaagct
    19201 tcggcttcgg tgtagtattg gttatcgagt ttacgctgaa tattactttt atatgcaaga
    19261 ccaaatttac cgatacttgt ttcatcatta tgcacagcac aaccagatat aagaaaaata
    19321 ctaattaatg atatagcagc tatctttttc atctcacctt cccccattaa ataccaacga
    19381 cactctctag tgtttaaata taataatggc atgattatta taattgaata ggattataat
    19441 aaatgttctg tacaacattt cctacataag taggaattac ggacattgag gcccttcagg
    19501 gtaactccat gggggcttta atatattata ttgaagatgc cactgtttag ttgaatatta
    19561 ggtatatgct cttttttgaa atttatcggt ggcagccgtt agtattcgct gtccccattg
    19621 caagctcctg gtggtaacca ctgaatcctc catacttgaa ctgacttttt atcctccgac
    19681 tttcatcctg ttctgactcc accttttgtt ttctgctcta cactatctac agaccaatca
    19741 taaaggcaca tacgatcatg gcagaatttc ccgccagctt actgattctt aatggtaaaa
    19801 gtactgacaa tctacccttg cgcgaagcaa ttatgctgtt gcgtgaggaa ggaatgacga
    19861 tccatgtgcg ggtcacctgg gagaaaggtg atgccgcacg atttgtagag gaggcccgga
    19921 agttgggcgt cgcaacggtg attgccggtg gtggcgatgg caccattaat gaagtttcta
    19981 cggcgttgat tcagtgtgag ggggatgaca tacccgcgct gggaattttg ccattaggaa
    20041 ccgccaatga ttttgccacc agtgtaggga ttcctgaggc actggataag gcgctgaaac
    20101 tggcaattgc cggtaatgcc attgcgatag atatggcgca ggtcaacaaa caaacctgtt
    20161 ttattaatat ggcgacaggc ggatttggga cgcgtattac cacagaaacg ccggaaaaat
    20221 taaaagccgc gctgggtggc gtctcttaca tcattcatgg cttaatgcgc atggatactc
    20281 tgcaaccgga ccgttgtgaa atccgcggtg aaaactttca ctggcaaggt gacgccctgg
    20341 tcattggtat tggtaacggg cgtcaggccg gtggcggtca acaattgtgc ccgaactcgt
    20401 taattaacga tggcttgctg caactgcgca tttttaccgg cgatgaaatt cttccggctc
    20461 tcgtatcaac cttaaaatct gacgaagata acccgaatat tatcgaaggc gcttcgtcgt
    20521 ggtttgatat acaagcccca cacgaaatca cttttaatct tgatggcgaa ccgttgagtg
    20581 ggcaaaactt ccatattgaa atacttccgg cggcgttgcg ttgtcgatta ccaccagatt
    20641 gtccattatt gcgttaatca aactattctc tcttgaacca gggagaatcc gtaatgccgg
    20701 atgcggtgta aacaccttat ccggcataca gaacaatact acaccgtaat taattttacc
    20761 cgtgtcttct gcaatgccaa tttatccacc tctgacacag catcatcggt aacaataatg
    20821 tcaatttttt cgattggcag tacctgatta aaaccgcgac gattaaattt cgatgaatcg
    20881 agaacggcaa tcactttatt tgcggcggtg accatcgccc cgctaatcga ataaccttca
    20941 ttaaaggtcg taataccatt aaccgcatca ataccatcag caccgacaaa cattaaatcg
    21001 gcattaatat cctgcaatga acgctcggca atggaaccat gcatcgagcg cgttttatgg
    21061 cggaccgtgc caccacagac gaccagagta atgtctttat tttcggaaag ggcaaacgct
    21121 gccgggagac tgttggtaat caccgtgata tttttagcgg tcattaatcc ttcggcgata
    21181 agcattgtgg tacttccgct gtcgagaata acggtcatcc cttcatggat cattttgact
    21241 gccgcctgcg ctattctgtt ttttggcgcg ctggcgagct gaaatcgctc tttaaacccg
    21301 acttcctggg tctcggtttc actattaccg gacattattt tcgccgcacc gccatgaaag
    21361 cgcgtaacca cgcctttttg ttcgagaaag cgcaaatcgg cacggattgt cgcttccgag
    21421 gcagcaaata ctcccgccag atcctgaaca agcacggttc cctgttcatt cactaattgg
    21481 atgatcttat tccttcgctc gaatgaattc atcgtcattt acccatattc aattgtggct
    21541 agtgtaaacg aagggggacc attggtgaat gatctgtttt gcacaatacc gggccagcat
    21601 cacgctggcc cgcggtttca tggaatgagc aacactttgc cctgcatggc attacgagcg
    21661 atgtcacgca ccgcctgggt gaagctttca aagcttccac ggtgagcgat taatggctcc
    21721 aggcttaact tacgttctgt cagcaaccgg ctcgccgttt cccactcctg ccccggccaa
    21781 gggctggaat agttcatcca actgccgata accgtcagct ctttacgcaa tattttgcca
    21841 aacgttgccg atgttaaatg cagatcctga tgcaacgtgc ccaccagcgc cagttgggca
    21901 tgaggaccgg caatctctac cgccagttcg acagtttgcg gtacgccagc cgtctcgagg
    21961 ataagctgat taaagcgcag ttcgcgtaaa acgctctgca tttgcggcgc gctcatttcg
    22021 ctactgttaa atgtttgcat cgcaccgaaa gattttgcca gtgccagttt ttctgagcta
    22081 atgtcgatcg ccgtcacact ctttgctccc agcgcgacag cgcactgaat agccagcagg
    22141 ccaatggttc cggcaccaat aataataacg tttttattct cacaaccttg cgctaaatga
    22201 aaagcatgca ggccaacggt aatcggctca ataaaagccc catcctcaat aggcatatcc
    22261 gtgggtagag caaagacatt ttttcgctta acgacaatat attcagcaaa tccaccatca
    22321 cgccgcgagc caataaaatc atattttgcg cactgggaat aaaacccttt caaacactct
    22381 ggacaagtaa aacagggtaa taacggcaca caggcaaccg catcgccagg gtgtaaatca
    22441 tcaacaccgg atcccaccgc atcaatatag ccgctaaatt catggcctaa cgttattgga
    22501 taataatgtg caccattttt aaatatcctg ggtaaatcgg aaccacataa gcccgagctg
    22561 gcaattttta cccgcacctc atcctgatgt ttaatttcag gaatgacgct ttttgcaacg
    22621 cgcacgatac catcagtatc attcactact gatttcataa aaactcctga tt
//
LOCUS       DAZPAB010000049        22456 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZPAB010000049 DAZPAB010000000
VERSION     DAZPAB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.049"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            82..2682
                     /locus_tag="ACHUPG_001698"
     CDS             82..2682
                     /locus_tag="ACHUPG_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFR0861355.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2673..3742
                     /locus_tag="ACHUPG_001699"
                     /pseudo
     CDS             2673..3742
                     /locus_tag="ACHUPG_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            3754..4294
                     /locus_tag="ACHUPG_001700"
                     /pseudo
     CDS             3754..4294
                     /locus_tag="ACHUPG_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4302..4817
                     /locus_tag="ACHUPG_001701"
     CDS             4302..4817
                     /locus_tag="ACHUPG_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0861356.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4783..5520
                     /locus_tag="ACHUPG_001702"
     CDS             4783..5520
                     /locus_tag="ACHUPG_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR0861357.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5631..6641
                     /gene="pyrD"
                     /locus_tag="ACHUPG_001703"
     CDS             5631..6641
                     /gene="pyrD"
                     /locus_tag="ACHUPG_001703"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR0861358.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6815..7357
                     /gene="zapC"
                     /locus_tag="ACHUPG_001704"
     CDS             6815..7357
                     /gene="zapC"
                     /locus_tag="ACHUPG_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR0861359.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="ACHUPG_001705"
     CDS             complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="ACHUPG_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR0861360.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8707..10815
                     /gene="rlmKL"
                     /locus_tag="ACHUPG_001706"
     CDS             8707..10815
                     /gene="rlmKL"
                     /locus_tag="ACHUPG_001706"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR0861361.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10827..12734
                     /locus_tag="ACHUPG_001707"
     CDS             10827..12734
                     /locus_tag="ACHUPG_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0861362.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12864..14117
                     /gene="pqiA"
                     /locus_tag="ACHUPG_001708"
     CDS             12864..14117
                     /gene="pqiA"
                     /locus_tag="ACHUPG_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR0861363.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14122..15762
                     /gene="pqiB"
                     /locus_tag="ACHUPG_001709"
     CDS             14122..15762
                     /gene="pqiB"
                     /locus_tag="ACHUPG_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR0861364.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15759..16322
                     /gene="pqiC"
                     /locus_tag="ACHUPG_001710"
     CDS             15759..16322
                     /gene="pqiC"
                     /locus_tag="ACHUPG_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR0861365.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16578..16745
                     /gene="rmf"
                     /locus_tag="ACHUPG_001711"
     CDS             16578..16745
                     /gene="rmf"
                     /locus_tag="ACHUPG_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR0861366.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="ACHUPG_001712"
     CDS             complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="ACHUPG_001712"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR0861367.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17402..19162)
                     /locus_tag="ACHUPG_001713"
     CDS             complement(17402..19162)
                     /locus_tag="ACHUPG_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0861368.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19347..19799
                     /gene="matP"
                     /locus_tag="ACHUPG_001714"
     CDS             19347..19799
                     /gene="matP"
                     /locus_tag="ACHUPG_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR0861369.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="ACHUPG_001715"
     CDS             complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="ACHUPG_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR0861370.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="ACHUPG_001716"
     CDS             complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="ACHUPG_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR0861371.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22005..22415
                     /gene="sxy"
                     /locus_tag="ACHUPG_001717"
                     /pseudo
     CDS             22005..22415
                     /gene="sxy"
                     /locus_tag="ACHUPG_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
BASE COUNT         5734 a         5272 c         5881 g         5569 t
ORIGIN      
        1 tatcgaacca tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc
       61 gtcttcagtt aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct
      121 ggtggagtgc catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca
      181 caagctgaaa cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat
      241 ttgtctcgtt tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt
      301 ctgaatcaga caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag
      361 gggattgccg cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg
      421 tttccggctt ttaaacaact ggacaaacaa gtgtgtgtgc cattggcgga gattattccg
      481 gatgccagcg taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc
      541 gccatcaaaa gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac
      601 gcgctattac tgggatattc gtttagcggg gctaacagta ttcatagcag cgcagacagt
      661 gattctggcg acagctattt tctgaattta aacagtggcg ttaatttagg cccatggaga
      721 ttacgcaaca attcaacatg gagtcgcagt agtggccaaa ccgcagaatg gaagaatctc
      781 agcagctatt tgcagcgggc ggttattcca ctaaaaggcg aactgaccgt aggtgatgat
      841 tatactgcag gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat
      901 gacaacatgc tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa
      961 agcaatgccc agataacgat taagcaaaat ggttacacca tttaccaaac ttatgtatcg
     1021 cctggtgctt ttgaaattag tgatctctat tccacgtcgt cgagcggtga tttgttagtt
     1081 gaaatcaaag aagcggacgg cagcgtcaat agctacagcg taccgttttc cagcgtgcca
     1141 ttactccagc gtcaggggcg aatcaaatac gcggtgacac tggcgaaata cagaaccaat
     1201 agtaatgaac agcaggagag caaatttgcc cagaccacgt tgcagtgggg cggaccgtgg
     1261 ggaacgacat ggtatggtgg tggacaatat gctgaatatt accgtgccgc catgtttggt
     1321 ctgggattta accttggcga tttcggagca atttcgttcg atgcgaccca ggcgaagagt
     1381 acgctggcag accaaagcga acataaaggt cagtcatatc gttttctgta tgccaaaacg
     1441 ctcaaccaat tgggcactaa ttttcaattg atgggctatc gctattcgac gtcgggtttc
     1501 tacacccttt ccgacaccat gtataaacat atggatggct acgaatttaa tgacggtgat
     1561 gatgaagata cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa
     1621 ctgcaggtca atatctccca gcaattaggc gagtacggtt cgttttattt aagtggtagc
     1681 cagcaaactt actggcatac cgatcaacag gatcggctat tacagtttgg ctacaacacg
     1741 caaattaaag atctctcgct gggggtttcc tggaactaca gtaagtcccg tggtcaacct
     1801 gatgctgatc aggtgtttgc actaaatttt tccctgccgc tcaatctgtt gctccccaga
     1861 agtaatgata gctataccag gaaaaaaaat tacgcctgga tgacctctaa caccagtatc
     1921 gataacgaag ggcacattac acaaaacctg ggtttaacgg agacactact cgatgacggt
     1981 aacctgagct acagcgtgca acagggatat aacagcgagg ggaaaacggc taatggtagc
     2041 gccagcatgg actacaaagg ggcgtttgca gatgcccgag tgggctacaa ctacagcgat
     2101 aacggcagtc aacaacaact gaactacgct ctttcaggca gtttagttgc ccattcacag
     2161 ggcattaccc tggggcaatc gctgggggaa actaacgtgc tgattgcagc accaggcgca
     2221 gagaatactc gtgtggcgaa cagcaccggg ctgaaaactg actggcgcgg atataccgtt
     2281 gtcccctatg ccacttctta tcgggaaaat cgaatcgcac ttgatgcggc atcgttaaaa
     2341 cgtaacgtgg atcttgaaaa tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt
     2401 ctggcggagt tcaatgccca tgcgggagca agggtattaa tgaaaacatc aaagcagggt
     2461 ataccgctgc gttttggcgc gatagcgacg ctggacggca tacagactaa tagcggcatt
     2521 attgatgatg atggctcgct ctatatgtcc ggtttgcccg ctcagggagc gataactgta
     2581 cgctggggcg aagctcccga tcaaatttgt catatcagtt accagcttac cgaacaacaa
     2641 attaactctg cgattacgcg aatggatgcc atatgcagat aatctttgga gaaaaatgcg
     2701 tgttattact acgactattt tttgccgccg tcttaatgct atggtgcgct caaactgctg
     2761 cttatatcgg gcagtgtcat accactcagg ggaatccgta tattggcgtc aattttggcg
     2821 ttaaaactct ggatgaagaa gaaaatacgg ctggagtagt taaagacaaa ttttatcagt
     2881 ggaacgaatc gaatgattat tatgtttcct gtgattgcga taaagacaat gtcagaagtg
     2941 gccgatgggc attcgccgcg gattcaccgt tagtctattt aggcgacaac tggtacaaaa
     3001 ttaatgacta tcttgccgcc aaagttttat tgcaggttaa aggcagttct cctactgcgg
     3061 ttcctttcga aaacgtgggc acaggggcag atacacgatg gcatatttgc gatcccggcg
     3121 gtcaacgttt aggtggccag ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat
     3181 tgcagccgtt cgttggttcg gtcgtcattc ctcctatggc gctggcgcga ttatttgaat
     3241 gctacaacat acccgcaggt gattcctgca cgactactgg tacaccggtt ttagtgtatt
     3301 acctgtctgg tacgatcaat tcacttggct catgttccgt caatgccgga gaaacaatcg
     3361 aggttgatct gggcgacgta tttgcggcta actttcgtgt tgtagggcat aagcctcttg
     3421 gggccagaac ggcagaactt gcaattccgg tcaggtgtaa cacgggaaac gcagggttag
     3481 ttaacgtcaa tctgagtctg acggcaacct cagaccccag ctatccccag gcgattaaga
     3541 cgtcacgtcc tggcgtgggc gtggtggtga ccgatagcca gcacaacatt atttcccctg
     3601 ctggtgggac actaccgctc tctattcctg atgatgcaga cagtatccgc gaatgaatgt
     3661 ctatccagtc agcacgacaa gcgtaccacc agaaaccggg cgatttgaag ccacggcaac
     3721 ggtgagaata aattttgatt aacgcgtgaa cgtatgaaga aaaaaacgat atatcagtgc
     3781 gttattttgt tctttagcct tcttaacatc catgtcggga tggctgggcc tgaacaagtc
     3841 agtatgcata tttatgggaa tgtggtcgat cagggctgtg atgtcgccac caaaagtgca
     3901 ttacaaaata ttcatattgg tgattttaat atcagtgatt ttcaggccgc gaataccgta
     3961 agcactgctg ctgatttgaa tattgatatc accggttgtg ccgctggtat tactggcgcg
     4021 gacgtccttt ttagcggcga ggctgacacc cttgcgccga cactgctcaa actaactgac
     4081 acaggcggaa gcggtggtat ggcaacgggg attgccgtgc attcttgatg cgcaaagtca
     4141 gcaagaaatc ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac
     4201 actcaaatat caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac
     4261 ggcggttctc tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct
     4321 ggattctgtt cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacgg
     4381 tcaacattag cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa
     4441 tcaatgtgca gttaggcgat gtctcgctga cacgtttttt tcatgggaat tatggcccgg
     4501 agaaatcttt catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgatgtca
     4561 ccttttcagg aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg
     4621 atgcagccag tgggctggca attgcgattc tggatgatgc gaagatattg attccgctta
     4681 accaggcgag taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc
     4741 aattgcgacc tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg
     4801 tacttcatta tgactaatac gtggaacaga ttagcgctct tgattttcgc cgttttatcg
     4861 ctgctggtgg cgggtgaatt gcaggcagga gtggtggtcg gcggaacgcg atttatcttt
     4921 ccggcagaca gagaatcgat atctatttta ctgactaata ccagtcagga atcttggctt
     4981 attaacagca aaatcaatcg cccaacgcgt tgggccgggg gggaagcgtc gacagtgcca
     5041 gcactattac tggccgctcc gccacttatt ctcctgaagc ccggtactac aggcacgttg
     5101 cgcttgctga gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta
     5161 agcattgcca gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc
     5221 tcggtattta aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat
     5281 caacaattac gctggacacg gaattcgcag ggtgtacaac tcactaaccc aacgccttac
     5341 tacattaacc tgattcaggt gagcgtaaat ggtaaagcgt taagtaatgc gggagtcgtg
     5401 ccgcctaaaa gccagcgtca gacaaactgg tgtcaggtaa tagcaccgtg tcatgtcgcc
     5461 tggcggacga taaatgatta cggcgggttg agtgcaaaga aggagcaaaa tctgccctga
     5521 aacaggttct gaaaacgttt gcgttttttt tgccgcaggt caattccctt ttggtccgaa
     5581 ctcgcacata atacgccccc ggtttgcaca ccgggaatcc aggagagttc atgtactacc
     5641 ccttcgttcg taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc
     5701 agcaattacg ccgcattacc ggaacaccgt ttgaagcact ggtgcggcag aaagtgcctg
     5761 cgaaacctgt taactgcatg ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc
     5821 ttgataaaga cggggagtgc attgatgcgt taggcgcgat gggatttgga tcgatcgaga
     5881 tcggtaccgt cacgccacgt ccacagcctg gtaatgacaa gccgcgtctc tttcgtctgg
     5941 tagatgccga aggtttgatc aaccgtatgg gctttaataa tcttggcgtt gataacctcg
     6001 tagagaacgt aaaaaaggcc cattatgacg gcgtcttggg tattaatatc ggcaaaaata
     6061 aagatacgcc agtggagcag ggcaaagatg actatctgat ttgtatggaa aaaatctatg
     6121 cctatgcggg atatatcgcc atcaatattt catcgccgaa taccccagga ttacgcacgc
     6181 tgcaatatgg tgaagcgctg gatgatctct taaccgcgat taaaaataag caaaatgatt
     6241 tgcaagcgat gcaccataaa tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg
     6301 aagacgaatt gatccaggtt gccgatagtt tagttcgcca taatattgat ggcgttattg
     6361 caaccaatac cactctcgat cgttctctgg ttcagggaat gaaaaattgc gatcaaaccg
     6421 gtggcttaag tggtcgtccg cttcagttaa aaagcacaga aattattcgc cgcttgtcac
     6481 tagaattaaa cggtcgctta ccgatcatcg gtgttggcgg catcgactcg gttatcgctg
     6541 cgcgtgaaaa gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta
     6601 aaggtccgcc gctgattaaa gaaatcgtta cccatatcta attatttctt cgaattcctc
     6661 tttataagat aatcagggct ttattttcag acctggttgt tttatattca tcgttgttgc
     6721 ttatttagac attttgtact tttattgttg aggttattaa gcgaagcgac aatggattgt
     6781 gttgctgcgg ttttataggg taggggagag gcagatgcga attaaaccag acgataactg
     6841 gcgttggtat tacgatgaag agcacgatcg aatgatgctc gatttagcca atggtatgct
     6901 atttcgctca cgttttgcgc gcaaaatgtt gactccagat gccttttctc ccgcaggctt
     6961 ttgtgttgat gatgccgcgc tctatttctc ttttgaagaa aagtgccgcg attttaattt
     7021 atctaaagac caaaaagccg aactggtgct gaatgcactg gtagcaattc gttatctcaa
     7081 accgcaaatg ccgaaaagct ggcattttgt ctcccatggt gaaatgtggg tgcccatgcc
     7141 gggcgatgcg gcctgcgttt ggttgagcga tactcacgag caggtcaatt tgctggtcgt
     7201 tgaatctggc gaaaatgccg cgctatgcct gctggcacaa ccctgcgttg ttattgcggg
     7261 tcgcgcgatg cagttgggcg acgccattaa aatcatgaac gacaggctga aaccgcaggt
     7321 gaatgttgac agcttcagcc tcgaacaggc agtctaacgc gtcagcttaa gggcagtctt
     7381 cggtacacag ctacagcaaa gaatggtgcc atcatcgccc attgctgatt ttttcagcgg
     7441 tgtgacttcg ccttctaaaa gctgaacacg gcaacttcca caaatgcccg cgcggcaaga
     7501 ataagggata cgaattccct gattttctaa ttgctccagc aacacttgtt ggttatttcc
     7561 acgaaatgcc tgtccctgcc agtcaatatc tacatttgcg tccggttgtt gcgtgatgtt
     7621 ggcggtatca tcagcggcag ctgcgccgta aatttttgcc ggagccgttg ccagaatttc
     7681 cacctcatcg ccaacgcgaa tcacgccgct attacgggca attaaattct gaccaaaatc
     7741 gacatcgccg ttatcctggg cagtgcggaa agattgcaat gtttttaatg gttcgcctgc
     7801 cggatgtttt tgcccttttt ctgggctgac ggtggtgaaa atacagcggc tacaaggttt
     7861 aaccacatca aacaccacat caccaatgcg aatcactttc cagctatctt cttcccaggc
     7921 tgacgcgccg gaaaccacca gattggggcg gaattgctcc atttttacac tggccggaca
     7981 acgttgttgg agatcacgta acgaggcttc gttagcaaga aggtaaggat agccatcagc
     8041 aaatgacaga ggtacagtgt tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca
     8101 gcgtaattgc acttcgcggg agaaaaatcc acttagccat ttgttgatcg cgtctggcgc
     8161 aattcgcgcg gtaaaatgtg tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc
     8221 agcaaaacga acatatgcgc tactgccatc tggtgcggtg agatgcaagc catcatgcac
     8281 aggtgaagga gtaaaccgta ccatctgggg aaactggcga gcggtaataa acgtaccgtc
     8341 aggttccgtg atcataaaga tgcgatcgaa ggccagacca ctgacatcag ccagagcatg
     8401 tgtaagacca atgccgcgca tcgatttaac aggatgaata aaaagccggg ttaatgtcgc
     8461 cacggtgcgg tcctcaaatg aaaataagcc ctcaacttta tgacatgacg cgcttattag
     8521 ctataatgcg caacaatttt cttagcgcct gtcccgttag gctattttat ttgtcagttt
     8581 ggccctgggt tgtgctcgaa atcctcacgt acttgtgtac gctccggttt ctccgcgctc
     8641 tccatgtcca gactgactgc aacaattacg cctactgcgg taggctctta gagtaaaagt
     8701 gacgatatga attctctgtt tgccagtacg gcccgtgggc tggaagagct gttaaaaact
     8761 gaactggaaa atctgggggc cgttgaatgc caggtggttc agggtggggt ccatttcaag
     8821 ggcgacacac ggcttgttta ccagagcctg atgtggagcc gcctggcctc gcgtattatg
     8881 ttgccgctgg gcgagtgtaa ggtttacagc gatttagacc tctatctcgg tgttcaggcg
     8941 atcaactgga cagagatgtt taatcctggc gcgaccttcg ctgtccactt cagtggtttg
     9001 aatgacacca tccgcaacag tcagtacggc gcgatgaaag tgaaagacgc gatcgtcgat
     9061 gctttcacac ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc
     9121 gttaacgtct ggctgcataa agaaaccgcc agtatcgctc ttgatctgag tggtgatggt
     9181 ttacatctgc gtggctatcg cgatcgtgct ggtattgcgc cgatcaaaga aaccctggca
     9241 gccgctattg tgatgcgatc cggctggcag tcaggaacac cgctgctcga tccgatgtgt
     9301 ggttccggta cgttgctgat tgaagcggcg atgctggcga ccgatcgcgc accaggcttg
     9361 caccgtgggc gttggggctt tagcggctgg gcgcagcatg atgaagctat ctggcaggaa
     9421 gtgaaagcgg aagcgcaaac tcgcgcccgt aaaggcctag ctgagtatag ctctcatttt
     9481 tacggttcgg acagcgacgc acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg
     9541 gggattggtg aactgatcac ctttgaggtg aaagatgtcg cgcaactgac caatccgctg
     9601 ccgaaagggc cgtacggtac agtgttgagc aacccgccat acggtgaacg tctggacagc
     9661 gaaccggcgc tgattgcgct gcatagtctg ctgggccgaa tcatgaaaaa ccagttcggt
     9721 ggctggaatc tctctttgtt tagtgcctcg ccggatctgc taagctgcct gcagctgcgt
     9781 gcagacaaac agtacaaggc gaaaaacggc ccgctggact gcgtacagaa aaattaccac
     9841 gttgccgaaa gcacaccaga cagtaaaccg gcaatggtag cggaagatta cgccaaccgc
     9901 ctgcgtaaga acctcaaaaa attcgagaag tgggcacgcc aggaagggat tgaatgttac
     9961 cgcctgtatg acgccgatct gccggaatat aacgttgccg ttgaccgtta tgccgactgg
    10021 gtggtggtgc aggagtatgc gccgccaaaa actattgatg cgcacaaagc gcgtcagcgt
    10081 ctgttcgata ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa caaactggtg
    10141 ctgaaaaccc gtgaacgcca taagggcaaa aaccagtacc agaaactggg cgagaagggc
    10201 gagtttcttg aagttaccga atataacgct cacttgtggg tgaacctgac ggattatctc
    10261 gatactggtc tgttcctcga tcaccgtatc gcccgccgta tgctcggtca gatgagcaaa
    10321 ggcaaagatt tcctcaacct gttttcttat accggcagcg ccaccgtgca tgcgggatta
    10381 ggcggtgcac gcagcaccac caccgtggat atgtcgcgta cttatctgga atgggcagaa
    10441 cgcaacctgc gtctgaatgg cctgactagg cgtgcgcatc gcctgattca ggccgattgc
    10501 ctggcgtggc tgcgtgaggc aaatgaacag ttcgatctga tctttatcga tccgccaacc
    10561 ttctctaact caaaacgaat ggaagatgcg tttgatgttc agcgcgatca tctggcgctg
    10621 atgaaagatt tgaaacgtct gctgcgtgca ggtgggacga tcatgttctc gaacaacaaa
    10681 cgtggcttcc gtatggatct cgacggcctg gcaaaactgg gactgaaagc acaagaaatt
    10741 acgcaaaaaa cgctctccca ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg
    10801 attaccgcag cctgaaagga atagtaatgt cattaatcag tatgcatggc gcatggctgt
    10861 cgttcagcga cgcgccgctt ctcgataacg cagaactgca tatcgaagat aacgaacgtg
    10921 tttgtctggt gggccgcaac ggcgcaggaa aatcgacgtt aatgaaaatc ctcaaccgtg
    10981 aacaagggct ggatgacggt cgcattattt atgagcaaga tttgattgta gctcgtctgc
    11041 aacaggatcc gccgcgtaac gttgagggta gcgtttatga tttcgttgcc gaaggcattg
    11101 aagaacaagc ggaatatctg aaacgctatc acgatatttc gcgcctggtg atgaacgacc
    11161 cgagcgagaa aaatctcaac gaactggcga aggttcagga acagctggat caccacaacc
    11221 tgtggcagct ggaaaaccgc atcaacgaag tgctggcgca actggggtta gatcctaacg
    11281 ttgcgctgtc gtcgctttcc ggcggctggt tgcgtaaagc ggcattagga cgcgcgctgg
    11341 tgagcaatcc gcgcgtattg ctgctggacg aaccgactaa ccatctggat atcgaaacca
    11401 tcgactggct ggaggggttc cttaaaacct tcaatgggac gattattttc atctcccacg
    11461 accgttcgtt tatccgcaat atggcgacgc gcattgttga tctcgatcgc ggcaagctgg
    11521 tgacctatcc agggaattac gaccagtacc tgcaggaaaa agaagaagcc ctgcgcgtgg
    11581 aagaactaca aaatgccgag ttcgatctca aactggcgca ggaagaagtg tggattcgcc
    11641 aggggatcaa agcacgccgt acccgtaatg aaggccgcgt acgcgccctg aaagcgatgc
    11701 gtcgcgaacg tggtgaacgt cgcgaagtga tgggtaccgc aaagatgcag gtggaagagg
    11761 ccagccgctc cggtaagatc gttttcgaaa tggaagacgt ttgctaccag gttaacggta
    11821 agcaactggt gaaagatttt tctgcccagg ttctacgtgg cgacaaaatt gccctgattg
    11881 gtccgaatgg gtgcggcaaa accacgctgc tgaaactgat gctcggtcag cttcaagcgg
    11941 acagcgggcg tattcacgtt ggcaccaaac tggaagtggc ttatttcgat cagcaccgcg
    12001 cggaactgga tcccgataaa acggtgatgg ataaccttgc cgaaggtaag caagaggtga
    12061 tggttaatgg caagccacgc cacgtattgg gctatttgca ggactttctg ttccatccga
    12121 aacgggcgat gacgccagta cgtgcgtttt ctggcggtga gcggaaccgc ttgctgctgg
    12181 cgcgtttgtt cctcaaacca agcaacttat tgattcttga cgaaccgacc aacgatcttg
    12241 atgtcgaaac gctggaactg ctggaagaac tgatcgacag ctatcagggc acggtattgc
    12301 tggttagcca cgatcgtcag tttgtcgata acaccgttac agaatgctgg atcttcgaag
    12361 gcggcggtaa aattggtcgt tatgttggcg gttatcatga tgcccgtggt cagcaagaac
    12421 aatatgtggc actcaaacag cctgcggtga aaaaaagcga agaagccgcc gcgccaaaag
    12481 cagaaactgt aaaacgcagc agtagcaaac taagctataa attgcagcgc gaactggagc
    12541 agctaccgca attgctcgaa gatctggagg cgaagctgga agccctacag acgcaagtgg
    12601 cggatgcttc cttcttcagt cagccgcatg agcagacgca aaaagtgctt gctgatatgg
    12661 ctgctgcaga gcaggagctg gagcaagcct ttgaacgctg ggagtatctt gaagcgttaa
    12721 aaaatggtgg ctgatcgcaa aatagtcgat ttaaacagag acagctgaca acggtaaaat
    12781 tgcctgatga actgggttta tcaggcatga aaaccaacac tttgttagtc atctgtaaaa
    12841 tagcgcatca ttaaggagta ccaatgtgcg aacatcatca tgccgcgaag cacatcctgt
    12901 gctcgcagtg tgacatgctg gtggcgttac cgcgccttga gcatggtcag aaagcggcat
    12961 gtccccggtg tggcacaacg ttaaccgtgg cgtgggatgc ccctcggcag cgtcccaccg
    13021 cctatgcgtt ggctgcactg ttcatgctgt tgctgtccaa cttgtttcct tttgtgaata
    13081 tgaacgttgc aggagttacc agtgaaatta cattactgga aattcccggc gtgctttttt
    13141 ctgaggacta cgccagcctc ggcacctttt tcctgttgtt tgtgcaactg gttcccgcgt
    13201 tttgtctgat aaccattctg ttactggtga atcgcgcgga attaccggtc cgtttaaaag
    13261 agcaactggc acgggtgctt tttcaactca aaacctgggg aatggcggag attttcctcg
    13321 cgggtgtgct ggtcagtttc gttaaactga tggcttacgg cagcattggg gtaggcagca
    13381 gctttctccc ctggtgttta ttttgtgtcc tgcaactgcg cgcttttcag tgcgttgatc
    13441 gtcgctggtt atgggacgac atcgccccga tgccagaact gcgccagccg ctaaaaccag
    13501 gcgtcacggg gatacgtcag gggctgcgtt cgtgctcctg ttgtacggca atccttcctg
    13561 ctgatgaacc cgtgtgcccg cgttgtagta ccaaagggta cgttcggcgt agaaacagcc
    13621 tgcagtggac actcgcgctg cttgtaacgt ccatcatgct gtatcttccg gctaatattt
    13681 tgcccatcat ggtgacggat ttattaggct cgaagatgcc gtcgacgatt ctcgctgggg
    13741 tcattctgtt atggagcgaa ggctcttatc ccgtcgctgc ggtgatcttt ctggccagta
    13801 ttatggtgcc aacgttaaag atgatcgcca tcgcgtggct gtgttgggat gccaaagggc
    13861 atggcaagcg cgacagtgaa agaatgcatt tgatttatga agttgttgag tttgtaggcc
    13921 gctggtcgat gattgacgtt ttcgttatcg cggtgctctc ggcgctggtg cgtatgggag
    13981 gtttaatgag tatttatccg gcaatgggtg cattaatgtt tgctttagtc gtcataatga
    14041 caatgttttc tgctatgacg tttgacccgc gtttgtcgtg ggatcgtcaa cctgaatcag
    14101 agcatgagga gtcctgacag tatggaatct aataatgggg aagccaaaat ccagaaagtg
    14161 aagaactggt ctcccgtgtg gatatttcct atcgtcacgg cgctcattgg ggcctgggtt
    14221 cttttttatc attacagcca tcagggaccg gaagtgaccc tgatcaccgc gaatgcggaa
    14281 ggaattgaag gtggcaaaac caccattaaa agccgtagcg ttgacgtcgg cgtggttgaa
    14341 agcgccacac tggctgatga tttgacgcac gttgaaatca aagcgcggct gaattccggt
    14401 atggaaaaat tgctgcataa agacaccgtc ttttgggtgg tgaaaccgca gattggtcgc
    14461 gaagggatta gcggcctggg aacgctgctg tctggagttt atatcgaact gcagccaggc
    14521 gcgaaaggca gcaaaatgga taaatacgat ttgctggact caccaccgtt ggccccgcct
    14581 gatgcgaaag gtatccgtgt gattctcgat agcaaaaaag ccgggcagct ctcgccagga
    14641 gatccggtgc tgttccgtgg ctatcgggta ggttcggttg aaaccagcac cttcgatacg
    14701 caaaaacgca atatcagtta tcaactgttc atcaatgcac cttatgaccg actggtgacc
    14761 agcaatgttc gcttctggaa agatagtggc attgcggttg atctgacgtc agcggggatg
    14821 cgtgtggaga tgggctcatt gacaacgctg cttagtggcg gtgttagctt tgatgtgccg
    14881 gaaggtctgg atttagggca gccagtggca ccgaaaacag ctttcgtttt gtatgatgat
    14941 cagaagagca ttcaggattc gttgtacacc gatcacattg attatctgat gttctttaaa
    15001 gattcggtac gcggtctgca accgggagct ccggtagaat tccggggtat tcgcctgggt
    15061 accgtaagca aagtgccatt ctttgcgccg aatatgcgtc agacatttaa cgatgattac
    15121 cgtattccgg tactgattcg tatcgagcca gagcggctga aaatgcagct tggcgaaaat
    15181 gcggatgttg ttgagcacct tggcgaattg ttgaaacgtg gtttacgcgg atcgctgaaa
    15241 accggaaacc tggtcactgg tgcactgtat gttgatctcg atttctatcc aaatacgcct
    15301 gcaataaccg gtattcgtga atttaatggt tatcagatta tcccgactgt tagcggcggc
    15361 ttggcgcaaa tccagcaacg actgatggaa gcgttggata agatcaacaa actgccattg
    15421 aatccgatga ttgaacaggc aaccagtacg ctttctgaaa gtcagcgcac aatgaaaaac
    15481 ctgcaaacga cgctggatag catgaacaag atcctcgcca gccagtcgat gcagcagttg
    15541 ccgacggata tgcagtcaac gttgcgtgaa ttgaatcgca gcatgcaggg cttccagcct
    15601 ggctccgcag cctacaacaa gatggtggcg gatatgcagc gccttgatca ggtgttgcga
    15661 gaactgcaac cggtgctgaa aacgctcaat gagaagagta acgcgctggt atttgaagcg
    15721 aaggacaaaa aagatccaga gccgaagagg gcgaaacaat gaaaaagtgg ctagtgacga
    15781 ttgcagcact gtggctggcc ggatgcagct ccggcgaaat taataaaaac tattaccagt
    15841 tacctgtggt gcagagcggt acacaaagta ccgccagcca gggcaatcgt ctgttatggg
    15901 tagagcaggt cactgttcct gactatctgg cggggaatgg tgtggtttat caaaccagtg
    15961 atgtgaagta tgtgattgcc aacaacaact tgtgggccag cccgttggat caacagttgc
    16021 gcaacaccct ggttgccaac ctgagtacgc aactgcccgg ctgggtggtt gcctcccagc
    16081 ctctgggaag cgcccaggac acgctcaatg ttaccgtaac ggagtttaac ggtcgctatg
    16141 atggcaaggt cattgtcagt ggtgagtggc tgttgaacca ccagggacaa ctgatcaaac
    16201 gtccgttccg tctggaagga gtgcaaactc aggatggtta cgatgagatg gttaaagtgc
    16261 tggccggtgt ctggagtcag gaagccgctt ctattgcaca agagataaag cgtctacctt
    16321 aattataaag atttgtaaat ataaccgtct ccggtatgtt gcctgaggcg gtttttttgt
    16381 ttctaacgtg cggaaaaatt tgttcctctt cacatttttt gtacaaccga catgcccgtg
    16441 tagctcacaa atatgacagt ggcgtgaatt ttgcgcattg acggcagtta tgattcgcgg
    16501 tattgcttaa ctgtgattgc acatttagta atcactgttt tcttttccac cagaaaccag
    16561 tatgagggaa acgaggcatg aagagacaaa aacgagatcg cctggaacgg gcacatcaac
    16621 gtggttatca ggccggcatc gccggacgct caaaagaaat gtgtccctat cagacgctga
    16681 gtcaaaggtc acaatggctg ggaggctggc gagaagccat ggcggacagg gtagtaatgg
    16741 cctgattctg tctctttaaa aagaaacctc cgcattgcgg aggtttcgcc ttttgatact
    16801 ctgtctgatg ataatcagaa ggcagacgta tcctggaaca gaccgacttt caggtcgctg
    16861 gcggtataga tcagacgaca atcaaccagc acttcgccat ctgccagacc cataatcaga
    16921 cgacgattaa caatgcgttt aaagtgaata cggtaggtca cttttttcgc tgtcggcagt
    16981 acctgaccag tgaatttcac ttcgccaacg cccagcgcgc ggcctttacc ttcgccgccc
    17041 agccagccga ggtagaaccc taccagctgc cacattgcgt ccaggcccag gcatcccggc
    17101 ataaccggat cgccaatgaa gtggcatccg aagaaccaca gatccggatt gatatccagt
    17161 tctgcttcaa catacccttt gtcgaagtta ccacccgttt cggtcatttt gaccacacgg
    17221 tctatcatca gcatgttcgg tgctggcaat tgcgggcctt tagcgccaaa cagttcaccg
    17281 tgaccagagg caagaaggtc ttcttttgta taggattcgc gtttatctac catgttctct
    17341 gtaagcctta ttttattgaa gcacgcagga tagctaacac gtgtacgctg aacaagtccg
    17401 atcagttcgg aataaaccag ttcagccaac gtaatggcca tggaaaacgg tgacgtcctt
    17461 cctgttgcga tgcttgcgcg atacgttcct ggatggtttg catcagcgtc gtttggcctt
    17521 cgccatccca caccagattt aataataacg gcagtgcgtc agtcacatcg tctactgccc
    17581 agatggtgaa tttgccttct tctaccgctt tcaccagttc actgtgaaga cttaaatggc
    17641 gaacgttagc agtggggata atgacacctt gtttccccgt taactcacgt tgctggcaaa
    17701 tagcaaagaa gccttcgatt ttctcattta aaccaccgac cggctggacg cggccgaact
    17761 gatcgactga acctgtgata gcgatactct gattcactgg cacatcagcg agggcgctta
    17821 tcagggcgca gagttcagcc atcgaggcac tatcgccatc cacttcactg tatgactgct
    17881 caaatgtcag cgatgctgag aaggggatct gttgctcaag ctgtagttcc gacatcagga
    17941 acgcctgcat gatcatcatc cctttcgcat ggatattgcc gccaagctcc gctttgcgtt
    18001 cgatgtcggt gaattcacca tcgccaatat gcacaacgca gctaatgcga gaaggttcgc
    18061 caaaagcgcg tggatgaccc ggaaattcaa tgaccgaaag ggcgttaatt tgcccgatgc
    18121 gttcgccttc ggtttcaatc aggatttgct cctgaaggat ctcatcctgc atacgttcag
    18181 cgaggaaacc ttcgcgccat tcacgctgct gcagcattaa gtttagctgc tcgccggaga
    18241 aggtgtcgcc atcacacagg gaggcgacct ctttacactg gcggaggatc cactgcgggc
    18301 taagcggaag tgtttcttgt tcaccggtgt agcgtgctgc ttcgcggata agtaccggcc
    18361 aagcatccgc tcccggggca ggtaagtgat tatgtctggc ggtaaatgtc acccagcgac
    18421 accactggct tactgactcc gcatcgacaa tctgcagagt atcttcaaat tcgctataaa
    18481 tagcctgctc tgaaagctct ggctccatct cctggaaatc agccaatgat tcgcgttcgc
    18541 ctaccagaat gactttcagc ttcaatggca ttgaaggcac agagacgggg agagggcgcg
    18601 actcatcaaa cgcaacccag tcaaaacgct cgcggttaac gatatttttc agccgcatcc
    18661 acagcagagg ttgcgccagc agtgtacgca aagagatgat gagaataccg ccatttgcct
    18721 gatgcaccag accaggctgc agggtaatgt cgccattaaa ctggcgcagg cagccaaaga
    18781 gttgctccgc ttctacccag tcggcagcga caacttgcgt taaagtcgca aaattatcat
    18841 ctgcactcac tgcgtgacgt aagcggatgg agtggccgga aacctcatag tgatcgccca
    18901 ccagttggcc tgcatcgctt tgtagcgtac gcgcggcatc ggcaataaga ttcagatact
    18961 cagactcttc cggggcctta gccagcataa aagaggagga tgctcgcgta tgcagcaact
    19021 gctccagcgc aaattgcagt cgcggttgag tatcactgaa taaaggatcg ttttcgtcaa
    19081 tcaaatgtgg ctgagcaaat atttcctgat agctatcggt atcaggaacc aggtcacgcc
    19141 atgcaagttt cgtaatggtc aaagttgatg ttttttagtc tgttgtcaaa gccgcgatta
    19201 taccgtaacc ggcactacag cacacgtaga aagcaccgac aatactcctg gctgggcgtt
    19261 aaagctcaca ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag
    19321 agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg
    19381 gttggaaatg gaagtatctg gtgaagaagc atcgcgaagg ggagttaatt acccgttaca
    19441 tagaagccag tgccgcccag gaagccgtcg atgtgttgct ctcgctcgaa aatgaacccg
    19501 tgctggtaaa tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc
    19561 agaccattcg ggccagacgg aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa
    19621 aatcgatcga tctggaattt attgtctggc aacgtctggc tggtcttgca cagcgtcgcg
    19681 gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat
    19741 acgcgaataa aatgtcttct cttaagcagg atctgcaggc attgctgggt aaggaataac
    19801 tgacgaaagt cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc
    19861 taccagacga taacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg
    19921 atctctacgc gacgatccgg agccaagcag tcgatcagtg cagcacgctg tttcacgttg
    19981 tcacaggtgt tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct
    20041 gccgggatac ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga
    20101 ccctggttgt aagcgtcaga accgatgcgg tcagtgtaac ccagaacaac tacggaaccg
    20161 tctttcggat ctaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc
    20221 ggtttcaggg ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg
    20281 gtctgtactt ccggtgccgg agctggagca actaccggag ctgcttcgcc ctgaccgaaa
    20341 cggtaggaaa cacccaggct cagcagaccg ttgtccggac gagtaccgat ggtgtttgcg
    20401 tcaccgatgt tgttggtcca ctggtattcc agacgggtag cgatttcagg agtgatcgcg
    20461 tactcaacac cacctgcgaa aaccggagaa acgccagtgt cgtggtcttt aaaggatgcg
    20521 ccaccaggta cgttagcctt ggtgtctgca cgccatacca taccacccag acgagtgtag
    20581 atgtccagat cgtcagtgat tgggtaaccc agtttagcgg tcagctgaac gccctgagct
    20641 ttgtatgcgc cgttgatgtt gtcgcctttg tacggcatac gacctaacca gtcgtaaccc
    20701 atttcaaaac caacatacgg gttaacctgg taaccaccaa aagcacctgc acccagttgg
    20761 ttttcgtggg tcggaccatt gttaggaata aaaccagtgt catggtactg ggaccagccc
    20821 agtttagcac cagtgtacca ggtgttatct ttcggagcgg cctgcgctac ggtagcgaaa
    20881 ccagccagtg ccactgcaat cgcgatagct gtctttttca ttttttgcgc ctcgttatca
    20941 tccaaaatac gccatgaata tctccaacga gataacacgg ttaaatcctt caccggggga
    21001 tctgctcaat attaactcta ccgatatctt cggcttatgc cgagcacccc tggcgatgta
    21061 aagtctacaa cgtagttgaa aacttacaag tgtgaactcc gtcaggcata tgaaaaaaaa
    21121 gtcttgtata aggtatgttt aatctttttg tcagcgacaa tttacagaaa aaaaacgcgg
    21181 aaaccgcttc agacaagcct tcgcaaggaa aattagtcac gactgaaagc attggctggg
    21241 cgacaaaaaa agttctagga ttaatcctaa atttacttaa tgatacaaat tagagtgaat
    21301 ttttagcccg gaaagttgtc tcgttgtgtg agaggatgcg cttaccggac gcataataaa
    21361 ccccatagcg ttaccttcat ttgccgcatc aacaagttca gcatgctctt cttcagtcaa
    21421 atcatctgtc aaccaaccga tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat
    21481 tgactccact gtgtggcaag gggagagctg gctaatctgc attactttcg ttaagggtag
    21541 cccagatgcc tgaacccatt cccgactcag tttttgttgc ggtgttaacc agagttgcca
    21601 gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg tcatcatggg
    21661 ctgatcttcg cgatagacaa cttcactgat aagcccggct gtagtgtttt ccgtagagac
    21721 acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt
    21781 gtacataatc aatccagccc ctgtgagtta ctgtatggat gtgcagtaca tccagtgaca
    21841 acaaagatca accctatttt cggaaagagc ctcgcaaatt ttgtcgttgg tgacgggaaa
    21901 acataaatta atcttgcccc ttaagaataa gttgcctatt ttcgtagtta acggatccgt
    21961 taatgtgaat cattctttta tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct
    22021 ataagcggat ctataaatca caagaatacc tggcaacgtt gggcacaatt gaataccgat
    22081 cattgtttgg cagttacagc ctgaccgttg acgacacggt gtttgcgatg gtttctgatg
    22141 gtgagttgta tcttcgggct tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg
    22201 tctggctgac atataaaaag tgtggccgat ccgttaccct caattactat cgggttgatg
    22261 aaagtctatg gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag
    22321 cgctgaaaga gaaaagcacg cgcaataccc gggaaagact gaaagatttg cccaatatgt
    22381 cttttcatct ggaagcgatt ctcggggagg tgggtagact ggccccctga atctccagac
    22441 aaccagtatc acttaa
//
LOCUS       DAZPAB010000050        22169 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZPAB010000050 DAZPAB010000000
VERSION     DAZPAB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22169
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.050"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(566..1657)
                     /gene="ychF"
                     /locus_tag="ACHUPG_001718"
     CDS             complement(566..1657)
                     /gene="ychF"
                     /locus_tag="ACHUPG_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR0861372.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1774..2358)
                     /gene="pth"
                     /locus_tag="ACHUPG_001719"
     CDS             complement(1774..2358)
                     /gene="pth"
                     /locus_tag="ACHUPG_001719"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR0861373.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2636..2914
                     /gene="ychH"
                     /locus_tag="ACHUPG_001720"
     CDS             2636..2914
                     /gene="ychH"
                     /locus_tag="ACHUPG_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR0861374.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2969..4648)
                     /gene="dauA"
                     /locus_tag="ACHUPG_001721"
     CDS             complement(2969..4648)
                     /gene="dauA"
                     /locus_tag="ACHUPG_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR0861375.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4773..5720)
                     /gene="prs"
                     /locus_tag="ACHUPG_001722"
     CDS             complement(4773..5720)
                     /gene="prs"
                     /locus_tag="ACHUPG_001722"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR0861376.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5871..6722)
                     /gene="ispE"
                     /locus_tag="ACHUPG_001723"
     CDS             complement(5871..6722)
                     /gene="ispE"
                     /locus_tag="ACHUPG_001723"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR0861377.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6722..7345)
                     /gene="lolB"
                     /locus_tag="ACHUPG_001724"
     CDS             complement(6722..7345)
                     /gene="lolB"
                     /locus_tag="ACHUPG_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR0861378.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7559..8815
                     /gene="hemA"
                     /locus_tag="ACHUPG_001725"
     CDS             7559..8815
                     /gene="hemA"
                     /locus_tag="ACHUPG_001725"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR0861379.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8857..9939
                     /gene="prfA"
                     /locus_tag="ACHUPG_001726"
     CDS             8857..9939
                     /gene="prfA"
                     /locus_tag="ACHUPG_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR0861380.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9939..10772
                     /gene="prmC"
                     /locus_tag="ACHUPG_001727"
     CDS             9939..10772
                     /gene="prmC"
                     /locus_tag="ACHUPG_001727"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR0861381.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10769..11161
                     /gene="sirB2"
                     /locus_tag="ACHUPG_001728"
     CDS             10769..11161
                     /gene="sirB2"
                     /locus_tag="ACHUPG_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR0861382.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11165..11974
                     /gene="sirB1"
                     /locus_tag="ACHUPG_001729"
     CDS             11165..11974
                     /gene="sirB1"
                     /locus_tag="ACHUPG_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFR0861383.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            12010..12864
                     /gene="kdsA"
                     /locus_tag="ACHUPG_001730"
     CDS             12010..12864
                     /gene="kdsA"
                     /locus_tag="ACHUPG_001730"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR0861384.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(13013..13120)
                     /locus_tag="ACHUPG_001731"
     CDS             complement(13013..13120)
                     /locus_tag="ACHUPG_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0861385.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(13525..14625)
                     /gene="chaA"
                     /locus_tag="ACHUPG_001732"
     CDS             complement(13525..14625)
                     /gene="chaA"
                     /locus_tag="ACHUPG_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR0861386.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            14895..15125
                     /gene="chaB"
                     /locus_tag="ACHUPG_001733"
     CDS             14895..15125
                     /gene="chaB"
                     /locus_tag="ACHUPG_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR0861387.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            15283..15978
                     /gene="chaC"
                     /locus_tag="ACHUPG_001734"
     CDS             15283..15978
                     /gene="chaC"
                     /locus_tag="ACHUPG_001734"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR0861388.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(16022..16375)
                     /gene="ychN"
                     /locus_tag="ACHUPG_001735"
     CDS             complement(16022..16375)
                     /gene="ychN"
                     /locus_tag="ACHUPG_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR0861389.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            16560..17954
                     /gene="ychO"
                     /locus_tag="ACHUPG_001736"
                     /gene_synonym="iatA"
     CDS             16560..17954
                     /gene="ychO"
                     /locus_tag="ACHUPG_001736"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR0861390.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(17955..18605)
                     /gene="narL"
                     /locus_tag="ACHUPG_001737"
     CDS             complement(17955..18605)
                     /gene="narL"
                     /locus_tag="ACHUPG_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR0861391.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18598..20394)
                     /gene="narX"
                     /locus_tag="ACHUPG_001738"
     CDS             complement(18598..20394)
                     /gene="narX"
                     /locus_tag="ACHUPG_001738"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR0861392.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            20733..22124
                     /gene="narK"
                     /locus_tag="ACHUPG_001739"
     CDS             20733..22124
                     /gene="narK"
                     /locus_tag="ACHUPG_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR0861393.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         5488 a         5499 c         5722 g         5460 t
ORIGIN      
        1 cagcggttaa aaaatttggt gaagattttt tgtaataatc ctaattagtt atgggatttt
       61 cgcttgatgt atctgcataa caaaaacaat atgcgccatt tttgcattac taataagaaa
      121 aagcaaaaaa catcgatatt catttttgtt caatggattt tctggcctat cgttattatt
      181 gtgctactgg ttatcatttt catcttgcca ggatcgcacg taatcacgtt attcggggac
      241 tttatctgcg ggatattacc cttcaggtaa gtccttttct cgccagcata ttggagcgag
      301 atcaatattg atcagctgtg aagagggctg atgtgtagtg gaacaaaaac tgacgttata
      361 tcactgctaa tgagatatca gtcatctgaa cacttatgtc tttaattatt ctcgtggttc
      421 actgtaggca ataagcacaa aagtgtagga tgttacaaga atgattagga ctcggtgaaa
      481 tgaaaaatcc acgcaattgc gtggatttta tatacttttg cgctcttcat gagatttagc
      541 gaaacctcat gagacaataa attaattaga cgttgaaaag gaagttcatc acatcgccat
      601 ctttcacgat gtaatcttta ccttctgcac gcattttgcc tgcttctttc gcgccttgtt
      661 cacctttgta agtgatgaaa tcttcaaacg agatggtttg tgcacggata aagccttttt
      721 caaaatcagt atggattttg cccgctgcct gcggcgcggt tgctccaacc ggaatggtcc
      781 atgcacgcac ttctttcacc ccagcggtga agtaagtttg caggttcagc agcttataac
      841 cggcacggat cacacggttc aggcccggtt cttccagccc aagctcctgc ataaactcgt
      901 cacgttcttc gtcgtccagt tcggcaatgt ctgcttcaac agcagcacaa accggaacca
      961 caacagaacc ttctttcgcc gcgatttcac gcacctggtc aagatatggg ttgttttcaa
     1021 aaccatcttc gttgacgttg gctatgtaca ttgttggttt cagcgtcagg aagctcaggt
     1081 aacgaatagc cgctttctct tcagcgctta aatccagcgc gcgcagcata cctgcgtttt
     1141 ccagctgggg caggcatttt tccaggaccg ccagctcagc tttcgcgtct ttatcgccac
     1201 ctttggcttt cttctgtacg cgatgaatcg cacgttcgca ggtgtcgagg tctgccagcg
     1261 ccagttcggt gttgataact tcaatatcgt cagccgggtt aactttgccg gaaacgtgaa
     1321 tgatgttgtc attttcaaag cagcgaacaa cgtgaccgat cgcttcggtt tcacggatgt
     1381 tggtcaggaa ctggttaccc agaccttcgc ctttcgatgc gccttttacc agaccggcga
     1441 tatcgacaaa ttccatggtc gtgggaagcg tacgttgcgg ttttacgatt tcagccagtt
     1501 gatccaggcg agggtcaggc attggtacga cgcctgtgtt cggctcaatg gtgcagaatg
     1561 gaaagttggc cgcttcaata ccggctttgg tcagcgcgtt gaacagggta gatttcccga
     1621 cgttgggcaa accgacgata ccgcatttga atcccatgat ttaaatcacc ttaatatctt
     1681 aataatcaac ctgttattgc taacagattg cagaaatgga aataactttg cctattatac
     1741 acggcactcg gcaaaaatgc cgcagacaac gacttattgc gctttaaagg cgtgcaatcg
     1801 gttcgttgct ttggtcaagc catctgtaaa ccacatttca gtacaacgcg ccgcttcgtc
     1861 gatggcttca tcaattaact tctgttcact aacaggcggt ttgcctaaca caaaaccgac
     1921 aactttattt ttatcgcccg gatgaccgat tccgatgcgt aaacggtgaa agttagggtt
     1981 attacccaat ttactgatga tgtctttcag tccattgtga ccaccatggc caccgcccaa
     2041 cttaaatttg gcgacgccag gaggcagatc cagttcgtcg tgggccacca gaatttcgtc
     2101 cggattaatg cggaaaaaac tggccatcgc cgcaacggct ttgccgctga gattcataaa
     2161 tgtagtcggg actaacaggc ggacatcttc gcttccaagg gtgactcgcg aagtataacc
     2221 aaagaattta gcctcttcgc gcagcggagc gcgcaaacgc tctgccagta agtcaacgaa
     2281 ccaggcacca gcattatgtc gcgttgcggc gtattcagca ccggggttcg ccaggccgac
     2341 aatcaattta atcgtcacgt ttttttgtcc tgagtgtgta cataactggc gcgtagttta
     2401 ctggttgcgg ccccgcttga caaaaaactg cgtatcaaat gcagataacg taataattgc
     2461 ctgagtggac tattagaaag tcaaggtgtt caggcgttta tttgtaaagt tttgttgaaa
     2521 taagggttgt aattgtgatt acgcccgcac ataacccaca gggtgttgtc tatactttac
     2581 acataaggaa gaggggtatt ccctgttaca acccagaaag ttccggaggt gacatatgaa
     2641 acgcaaaaac gcttcgatac tcggtaacgt gctcatgggg ttgggtctgg tggtaatggt
     2701 ggtcggcgtg gggtattcaa tcctcaacca gttaccacag tttaatatgc cccagtattt
     2761 cgcacatggt gcagtgctaa gtattttcgt cggtgccatt ctctggctgg cgggtgcccg
     2821 tgttggcggg catgaacagg tgtgcgaccg ttactggtgg gttcgtcact atgacaaacg
     2881 ttgccgccgt agcgataatc gccgtcatag ctaacaaata atgcagtttg ctgaccagtc
     2941 agtttgcgct gactggtcaa tctcgtactt ataaatccgc catcgccgcg cgacgattcg
     3001 ggaagaacgc gaggcgtccc gggatcggtt gaatgcctgc gcgagccata gttcgcagtg
     3061 gctggaattc cacgttgcac acgcgcagtt cacatccttc gggcagacgc ttcacaaaac
     3121 gctggaacgc atcaagacca ccagcatcaa gtaccggaac ggcatcccac ttcagaatca
     3181 caatccgttt gccttcaaga cgtgactcca ggtccgtgaa taagccttca gcagcagcaa
     3241 aaaacagcgg gccaataacg cgcagaacca ggacatcgtc tggaacatct acgactaccg
     3301 gtgccaggcg agtcatacgt gcgatacgac gcataaacag cagcgatgcc agcacgatcc
     3361 ccacgctgat ggcaataacc atatcaaaca gcacggtcag tgacatacac agcagcatga
     3421 cgatgatgtc atctttcggc gcatgacgca gcaagtcgac cactttgtgc gcttcactca
     3481 tgttccacgc caccatcaac agcagggctg ccatggcgga aagcggcagc caggagagca
     3541 gtggtgccag taccagcagg gcgagaataa ccagaataga gtggatcacc gccgagatag
     3601 gggaagttgc cccggcacgg acgttagcgg cagaacgcgc gatggcagca gtagcggtaa
     3661 taccaccaaa gaatggagcg atgatattcc ccagcccctg accgaccagt tcactattcg
     3721 ccttgtgctt agtcccggtc ataccatcca gtaccacagc gcagagcaga gattcgatcg
     3781 ccccgagcat tgccattgag aatgccgcag gcagcagggt gcgaatagaa tcccaggtta
     3841 gcgtgaattc tgaattaggc agatcccacg gcagcaccag ttgcggcagc agttgcggaa
     3901 taccgttacc ctgagaacca tcggccagga cgtagtggaa ttgcgaaccg atggtagcaa
     3961 catgtccgcc gagcaggtta acaatcccca tcaccgcgca accagccagc aaggccggaa
     4021 ggtgaccggg taaacgaatg cccagacgcg gccagaaaac cagaatgcca agcgtcacaa
     4081 tgccaatggc agcatcaccc acattaatgg tcggcagcgc cataaataat gcgccgactt
     4141 tttgtagata atgttccggg acatgggcca tttgcagacc gagaaaatct ttaatctgca
     4201 tggtaccgat ggtgatcccg atacccgagg tgaaacctaa ggtgacggaa accggaatat
     4261 actcaatcag acgaccaaag cgtgccagcc ccatcagaat caaaaagatt cccgacaaca
     4321 aggtcgcaac cagcagtcct gccagtccaa actgttgcga aacgggatag agaattacca
     4381 caaatgccgc agtcggaccg gaaacgctaa agcgtgaccc acccgttaga gcaatgacaa
     4441 tccccgcaac agctgcggta tataaaccgt actggggggc cacaccacta ccaatagcca
     4501 acgccatcgc cagcgggata gcaataatcc cgacggttat cccggcaatc aggtcacggg
     4561 taaaccgtgc ggcagtatat ttttctttcc agcaaacgtc gatcagagcg cggaaaggca
     4621 tcacatgtga ggaaaatatt ttgttcacaa taatgtttca tccgtgagcg catcatctgt
     4681 caactaaatg gcaggtgaag gaggcatagg tcatacaaat ggatattaca gacaaaaaaa
     4741 cccgccgcag cgggtctttg agccgggttc gattagtgtt cgaacatggc agagatcgat
     4801 tcttcgttgc tgatacgacg aatcgcttcg gccagcatac ctgacagggt cagagtacgc
     4861 acgttcggca gtgatttgat ttcatcgctc agcggaatgg tatcgcagac aacgacttca
     4921 tcaattacag agttacgcag gttgttcgcc gcgttgccag agaagatcgg gtgagtcgcg
     4981 tacgcaaata cacgtttagc accacgttct ttcagcgctt cagcagcttt acacagcgta
     5041 ccgccagtgt cgatcatatc atcgaccagt acgcagtcac gacctgcaac gtcaccgatg
     5101 atatgcatca cctgggaaac gttcgcacgc ggacgacgtt tgtcgatgat tgccatatcg
     5161 gtatcgttca gcagcttagc gatagcgcgg gcacgcacaa cgccgccgat gtccggagaa
     5221 accacaattg ggttatccag attcagctgc agcatgtctt ccagcaggat cgggctacca
     5281 aatacgttat caaccggaac gtcgaagaaa ccctgaatct gttcagcgtg cagatccact
     5341 gtcagcacac ggtcaacacc gacgctggag aggaagtctg caaccacttt cgcagtgatt
     5401 ggtacacgag cggaacggac gcgacggtcc tggcgcgcat agccaaagta ggggataaca
     5461 gcggtgatac gacctgcgga agcacgacgc agggcatcaa ccataacgac taattccatc
     5521 aggttgtcgt tagtaggggc acaagtggac tggatgatga aaatatcacc accgcgtaca
     5581 ttttcgttaa tttgtacgct gacttcgccg tcgctaaagc gacctacagc ggcgtcgccg
     5641 agtgaagtgt acaggcggtt ggcaatacgt tgtgctagta ccggggtggc gttaccagca
     5701 aaaagcttca tatcaggcac gagaagaacc tcaggcatgc gtccattggt ggaaagaatc
     5761 tgccgaaaac tgtgcgggcc aggcatgatc ctttccaggc ggtgtattaa agagcgcgat
     5821 gcaacgtctg gaacaaggtg acgttgtcac cgaaactcag cttgcccggc ttaaagcatg
     5881 gctctgtgca atggggaaag attaacgcct ttcgccacaa agccattgag ccattccggg
     5941 gcttgctcta gcacctggcg ggcttcagac tctgtatcaa attcagcaaa gacacaggcc
     6001 cctgtcccag tcaggcgtga cggggcgtat tctaacagcc aggaaagcac cgcatcaacc
     6061 tcgcgaaaac gttttcttgc gataacctcg caatcattgc tgaattcaca ttttagcaac
     6121 gtttctattg acctttttgg cgtattgcgc gggagttcag gatctttaaa aatcaccgga
     6181 gtcggaatac ttacaccagg gtgcgccacc agataccact tctctggcgg atccaccggc
     6241 gttagtattt caccaacgcc ttcggcaaac gcggcatgcc cccgaacaaa gacaggaaca
     6301 tctgcgccca gcgtcagccc catttccgcc agctcatcca tgcttagccc gcattgccag
     6361 agatgattta atgccaccag gaccgtcgcg gcattggatg aaccaccgcc gagaccgccg
     6421 cccatcggca aacgcttgtc aatgctgata ttcgcaccgc ttcccgtcgg aagacgcccg
     6481 ctgtctgccg cagttttcat caacaaccgc gctgcgcgaa cgatcaggtt atcttcatgt
     6541 tccacgcctt caacgggcgt taacagacga atatccccat cgtcacgaag ctcaatgctg
     6601 atggtgtcgc cgtaatcaag aaactgaaac agcgtttgca gcgtgtggta accatccgca
     6661 cgctgaccgg taatgtataa aaacagatta agttttgccg gagagggcca ctgtgtccgc
     6721 attatttcac tatccagtta tccattttta acttgatgcg ttgaccaccg tcggtgagtt
     6781 ccatattggc tggcatcgca ggttgcgttt tggtgtcata accaccataa acaaccttcc
     6841 agtttttgcc attctggctg taggtaattt cgctcaggcg gtactggtcg tccagtttgt
     6901 agtcggttgc atcacccggt aaacctaaaa tccactggcg caagctgttg agcggaattg
     6961 gcattccggt caatttgcca atcatctctt cggcgtcatc ggcggtataa cgctgacctt
     7021 tattgtcgac taactgcacg ttacccggtt gagcattcag ctccagttcc gtgctgccca
     7081 atgggttagt gagcagcaga cggtagcgat cctggccggt ttgctgccag aaaaagcggg
     7141 cgtacacttt ttgttggtca gaaatataag cgaacgcgcc gcgagtctga tactgattaa
     7201 gattgcgcac gtcttgctga tgctgacgcc attgtggcga atccgggctt ttgccaggac
     7261 ctttgggcgt ggtaacggaa caggcagtga gcacaagagc agccagcggt agcaggcgga
     7321 taagacgaaa atcgggcagg ggcatagtga tgacaagtcc ttgagatacg ttgcagttat
     7381 aacccttaat gctagcgtta ccgtccgcta tcgtctatgt tcaagttgtc ttaattgcca
     7441 gaatctaacg gctttcggca attactccaa aagggggcgc tctcttttat tgatcttacg
     7501 catcctgtat gatgcaagca gactaaccct atcaacgttg gtattatttc ccgcagacat
     7561 gaccctttta gcactcggta tcaaccataa aacggcacct gtatcgctgc gagaacgtgt
     7621 atcgttttcg ccggataagc tcgatcaggc gcttgacagc ctgcttgcgc agccgatggt
     7681 gcagggcggc gtggtgctgt cgacgtgcaa ccgcacggaa ctttatctta gcgttgaaga
     7741 gcaggataac ctgcaagagg cgttaatccg ctggctttgc gattatcaca atcttaatga
     7801 agaagatctg cgtaaaagcc tctactggca tcaggataac gacgcggtta gccatttaat
     7861 gcgtgttgcc agcggcctgg attcactggt tcttggggag ccgcagatcc tcggtcaggt
     7921 taaaaaagcg tttgtcgatt cgcaaaaagg tcatatgaag gccagcgaac tggaacgcat
     7981 gttccagaaa tctttctctg tagcgaaacg cgttcgcact gaaacagata tcggtgccag
     8041 tgctgtgtct gtcgcttttg cggcttgtac gctggcgcgg cagatctttg aatcgctctc
     8101 tacggtcaca gtgttgctgg taggcgcggg cgaaaccatc gagctggtag cgcgtcatct
     8161 gcgcgaacat aaagtacaga agatgattat cgccaaccgc actcgcgaac gtgcccaaat
     8221 tctggcagat gaagttggcg cggaagtgat tgccctgagt gagatcgacg aacgtctgcg
     8281 cgaagccgat atcatcatca gttccaccgc cagcccgtta ccgattatcg ggaaaggcat
     8341 ggtggagcgc gcattaaaaa gtcgtcgtaa ccaaccaatg ctgttggtgg atattgccgt
     8401 tccgcgcgat gttgagccgg aagttggcaa actggcgaat gcttatcttt atagtgtgga
     8461 cgatctgcaa agcatcattt cgcacaacct ggcgcagcgt aaagccgcag cggttgaggc
     8521 ggaaactatt gtcgctcagg aaaccagcga atttatggcg tggctgcgag cacaaagcgc
     8581 cagcgaaacc attcgcgagt atcgcagcca ggcagagcac gttcgcgatg agttaaccgc
     8641 caaagcgtta gcggcccttg agcagggcgg cgacgcgcaa gccattatgc aggatctggc
     8701 atggaaactg actaaccgct tgatccatgc gccaacgaaa tcacttcaac aggccgcccg
     8761 tgacggggat aacgaacgcc tgaatattct gcgcgacagc ctcgggctgg agtagcagta
     8821 catcattttc tttttttaca gggtgcattt acgcctatga agccttctat cgttgccaaa
     8881 ctggaagccc tgcatgaacg ccatgaagaa gttcaggcgt tgctgggtga cgcgcaaact
     8941 atcgccgacc aggaacgttt tcgcgcatta tcacgcgaat atgcgcagtt aagtgatgtt
     9001 tcgcgctgtt ttaccgactg gcaacaggtt caggaagata tcgaaaccgc acagatgatg
     9061 ctcgacgatc ctgaaatgcg tgagatggcg caggatgaac tgcgcgaagc taaagaaaaa
     9121 agcgagcaac tggaacagca attacaggtt ctattactac caaaagatcc tgatgacgaa
     9181 cgtaacgcct tcctcgaagt ccgtgccgga accggcggcg acgaagcagc gctgtttgca
     9241 ggcgatctgt tccgtatgta cagccgttac gccgaagccc gccgctggcg ggtagaaatc
     9301 atgagcgcca gcgagggtga acatggtggt tataaagaga tcatcgccaa aattagcggt
     9361 gatggtgtgt atggtcgtct gaaatttgaa tccggcggtc atcgcgtgca acgtgttcct
     9421 gctacggaat cgcagggtcg tattcatact tctgcttgta ccgttgcggt aatgccagaa
     9481 ctgcctgacg cagaactgcc ggacatcaac ccagcagatt tacgcattga tactttccgc
     9541 tcgtcagggg cgggtggtca gcacgttaac accaccgatt cggcaattcg tattactcac
     9601 ttgccgaccg ggattgttgt tgaatgtcag gacgaacgtt cacaacataa aaacaaagcg
     9661 aaggcgcttt cagtgctcgg tgctcgcatc cacgctgctg aaatggcaaa acgccaacag
     9721 gccgaagcgt ctacccgtcg taacctgctg gggagtggcg atcgcagcga ccgtaaccgt
     9781 acttacaatt tcccgcaggg gcgcgttacc gatcaccgca tcaacctgac gctctaccgc
     9841 ctggatgaag tgatggaagg taagctggat atgctgattg aaccgattat ccaggaacat
     9901 caggccgacc aactggcggc gttgtccgag caggaataat ggaatatcaa cactggttac
     9961 gtgaagcaat aagccaactt caggcgagcg aaagcccgcg gcgtgatgct gaaatcctgc
    10021 tggagcatgt taccggcaga gggcgtactt ttattctcgc ctttggtgaa acgcagctga
    10081 ctgacgaaca atgtcagcaa cttgatgcgc tactgacacg tcgtcgcgat ggtgaaccca
    10141 ttgctcattt aaccggggtg cgagaattct ggtcgttgcc gttatttgtt tcgccagcga
    10201 ccttaattcc gcgcccggat acggagtgtc tggtggagca ggcactggcg cggttgcctg
    10261 aacaaccttg ccgtattctc gatctcggga cgggtaccgg ggcgattgcg cttgcgctgg
    10321 ctagcgagcg cccggactgc gaaattatcg ctgtagatcg tatgcctgat gctgtctccc
    10381 tggcacaacg taatgcccag catctggcga tcaaaaatat ccacattctg caaagcgact
    10441 ggtttagcgc gctagccggg cagcagtttg cgatgattgt cagcaatccg ccgtatattg
    10501 acgagcagga cccacatctt caacaaggcg atgtccgctt tgagccgctc actgcgctgg
    10561 ttgcggcaga cagtggaatg gcagacatcg tgcatatcat cgaacagtcg cgtaacgcgc
    10621 tggtatccgg cggctttctg cttctggaac atggctggca gcagggcgaa gcggtgcgac
    10681 aagcatttat cctcgcgggg tatcatgacg tcgaaacctg ccgtgactat ggtgataacg
    10741 agcgcgtaac gctcggccgc tattatcaat gacaagtttt tctacactgc ttagtgttca
    10801 tcttattagt atcgcgcttt ctgttgggct attaacctta cgtttctggc tacgttatca
    10861 gaagcatcca caggcatttg ctcgctggac gcgcattgtg ccgccggttg tcgatacggt
    10921 gttattgtta agcggcattg cgttgatggc taaagcgcac atcctgccat tttccgggca
    10981 ggcacagtgg ctgactgaaa agctgtttgg agttatcatt tatatcgttt tgggttttat
    11041 tgcactcgat tatcgtcgta tgcacagtca gcaggcgcgc attattgcct tcccgctggc
    11101 gttggtggtg ctgtacatca tcattaaact cgccaccaca aaagtaccgt tactggggta
    11161 agtcatgaga tcgttagctg atttcgaatt taataaagcg ccattgtgcg aaggcatgat
    11221 cctggcttgc gaagcaatcc gccgcgattt tccctcgcaa gatgtttacg acgaactgga
    11281 gcgtctcgtt agtctggcga aggaagaaat cagccagctt ctgcctttag aagagcagct
    11341 ggaaaaactg atcgcgctgt tttacggcga ctggggattt aaagcctcac gcggcgttta
    11401 tcgtctttcc gatgcattat ggctggacca ggtgttaaag aatcgacagg gcagtgcggt
    11461 atcattaggt gcggttttat tatgggtcgc gaatcgtctc gatttgccgc tgctgccggt
    11521 gattttccct acgcagctga tattgcgcat tgaatgtccg gatggcgaaa tttggctgat
    11581 taatcctttt aacggtgaat cgttaagcga acatatgctg gacggatggt taaagggaaa
    11641 tatcagcccg tcggcggaac tgttttatga agaccttgat gaagctgata acattgaggt
    11701 aatccgcaaa ttgctggata cactcaaagc ctcgttgatg gaagaaaatc agatggagct
    11761 ggcgttacgc accagcgaag ctttattaca attcaaccct gaagatccct atgaaattcg
    11821 cgatcgtggg ttgatttatg cgcaactgga ttgcgaacac gttgcgttga acgatttaag
    11881 ttatttcgtt gaacggtgtc cggaagaccc gatcagcgaa atgatccgtg cgcaaataaa
    11941 taacatcgcg cataaacata ttgtgctgca ttaattaatc gacattttac tcaagattaa
    12001 ggcgatccta tgaaacaaaa agtggttagc attggcgaca tcaacgtagc aaatgacctg
    12061 ccgttcgtac tgtttggcgg tatgaacgtg ctggaatctc gcgatctggc gatgcgcatt
    12121 tgcgagcact acgtaactgt gacccagaaa ctgggtatcc cttacgtgtt caaagcctct
    12181 tttgacaaag ccaaccgctc ctccatccac tcttatcgtg gaccgggcct ggaagaaggg
    12241 atgaaaatct tccaggagtt gaagcagact tttggcgtga aaattatcac cgacgttcac
    12301 gaaccaagtc aggcacagcc cgttgctgat gtcgtggatg tgattcagtt gccggcgttt
    12361 cttgctcgcc agactgacct ggttgaagcc atggcgaaaa ccggtgcggt aattaacgtc
    12421 aagaaaccac agtttgtcag cccgggacag atgggtaata tcgttgataa attcaaagaa
    12481 ggcggcaacg aaaaagtgat tctttgcgat cgtggtgcta acttcggcta tgacaacctg
    12541 gttgtcgata tgctgggctt cagcattatg aagaaagtgt ctggtaactc gccggtgatt
    12601 ttcgacgtga cccacgcact gcaatgccgc gatccgtttg gcgcagcttc cggtggtcgt
    12661 cgtgctcagg tggctgagct ggcacgagcc ggtatggcgg taggtctggc ggggctgttt
    12721 attgaagcgc atccggatcc ggaacatgcg aaatgtgatg gtccatccgc gctgccgctg
    12781 gctaaactgg aaccgttcct caagcagatg aaagcgattg atgatctggt gaaaggtttc
    12841 gaagaactgg ataccagcaa gtaatctttt ttgcttgaaa aataaagtct tagcgttctg
    12901 cgttaagact tttttcatgg gtgccagata caaaaaaggc cgcaggctgt tacccctgcg
    12961 gccggtttcg ggcgcatatt gccatcacgg cagcctgacg cccgttttca ccttacttcc
    13021 ggttacgcca ccagctgaca atcgctgcgg caataatccc cgccaggatc ggtgctgcca
    13081 ggtcgtgcca gaaagtcatg gcaaactgcg cgagcgtcat atagccgcct tgttgtaatg
    13141 acaacatttt gcggctattc ttgaagtgtc tggtttcaag attagccccc gttttgttgt
    13201 caggttttac ctctcaacgt gcgggggttt tctctttcca gcaaccaatg ccaccaggga
    13261 taaagccccc gcaacattgc gcctcaccgg ataattccgg cttggtgtgg atactacgtc
    13321 tcaattcatc ttcacttcat ccctgaaatg tttgcaataa agagtacatt ccggcttttc
    13381 aacagctgtt gcagtggttt cgtgcaggtt gtggtacagg ctcgcaattc tgattaacga
    13441 gctgtccagg tagtatgaag aaatacaata agcaggaatt tatcttatct ctcccgattt
    13501 ccgtcatcgg aggagatata actgtcaggc aaatatcgtc atcaaatagg cggcaaacag
    13561 tgccagatgc gctgcgccat tgagcacgtt agtacgtccg gtggagaagg agatatggca
    13621 cagcactaaa gaggccacca tcaccaccat ttctggcgca ccaagtgcaa actgcaattc
    13681 gttacccgtc ataaaggcaa ttagcgtgac gacaggtacg gtaagcgaaa ttgttgctaa
    13741 cactgaacca aagaacagat tcatcgcgcg ctgaacctgg ttgttcaaca ctgcttttaa
    13801 cgcacctaaa ccttccggcg acagaatcaa cagtgccacc aggaagccag taaaggcaac
    13861 aggggcattc atgctgtcga gcaatgtctc cagcgagctg gcgttcattt tggtgactgc
    13921 aataacggca atcagatgga taatcaacca gatagcatgc cacaggctgc tatgggcaga
    13981 cgttttaccg tggtgcgggt cgtcgtcatc actgtcatct tcgtgctcgt agacaaacaa
    14041 actttgatgc gttttggcct ggatcagcaa aaatacgcca tacattgccg cagaaattaa
    14101 tgctaccagt aacgcctgac cggttgaaaa attcgccgca ggcagagcca ttggaaatac
    14161 cagtacgatt atcgccaggg ggaacagcgc aattaaatac tgcttgatac caaacagatt
    14221 catatattgg gtggcaaact tacgaccgcc caacaataat gaaaagccaa ccagcccacc
    14281 ggtaacaatc ataatgattg aatagagcgt atcacgcatt agcgttggcg cggcgtcgcc
    14341 ggttgccatt aaagctgaaa tcaaactgac ttcaagaata accactgaaa ggctaagaat
    14401 aagcgaaccg taaggttctc ccaggcgatg ggctaatacg tccgcatgac ggacaacact
    14461 aaaggcgcta cttaaaatac caataagcgc aagaagattg atggcaatga ccactggtag
    14521 tgtctggcta cttccccaca ggaacagcac taccagcacc agaaccggga aaataagcga
    14581 agtctccttg tggcgggttt ttaccgcctc ttgagcattt gacattatgg ttatcccttt
    14641 gcagatgaat ttatcgaaaa tgtaaaaaat aggtaggaaa aataacagaa agtgtctgga
    14701 tatcggtaac attttacgaa tttttacccc gctgtcgatt ttttactcat tggggcataa
    14761 aaataagtat tacgtttaga caatgtttgt tttagcgttt cttaagaaca gtctgacatg
    14821 aaaattctta tgttttggca agagtagata ttgttgacca cacttaatgt tcaactttgt
    14881 aaaaggagtc aacgatgccg tataaaacga aaagcgatct gccggaaagc gtaaagcacg
    14941 ttctaccgtc tcatgcccag gatatctata aagaagcgtt caacagcgca tgggatcaat
    15001 ataaagataa agaagatcgg cgtgatgacg ccagtcgcga agaaaccgcg cataaagtgg
    15061 cctgggctgc tgtgaagcat gaatatgcca aaggggatga tgataaatgg cataaaaaat
    15121 cgtaaaaccg gtagcttagt taaagctatt cgtgcggtgt tgccttgcaa gtggtccgtg
    15181 gattgcatat tgttccgtta gtggtttcaa aatgagcggt aaaaatgtcc ggaagacacc
    15241 aaaaagttgt cgcagggaag tatgcagtgg cggaggtgta aggtgataac gcgtgatttc
    15301 ttgatgaatg ccgattgtaa aacggcattt ggtgccattg aagaatcact cttatggtca
    15361 gcagaacaac gggcggcttc gctggcggcg acgctggctt gtcgacctga tgagggaccg
    15421 gtgtggatct tcggttatgg atcgttgatg tggaatccgg cactggagtt taccgaatca
    15481 tgcaccggta cactggttgg atggcatcgc gcgttttgcc tgcgcctgac cgccgggcgc
    15541 gggactgcgc accagccggg acggatgctt gcactgaaag agggcggacg caccacaggc
    15601 gtcgcctatc gactaccaga agagacgctg gagcaggaac taaccctgtt gtggaagcgc
    15661 gagatgatta ccggctgtta tctgccaacc tggtgtcagc ttgatcttga tgatggacgc
    15721 acagtaaacg ccattgtgtt tattatggac ccgcgacatc cagaatatga atctgacact
    15781 cgcgctcagg tcatcgcgcc gttgattgcg gcggcgagcg gtccgctggg aaccaatgca
    15841 caatacctgt tttcactgga acaggagctt atcaaactgg gaatgcagga cgatgggctg
    15901 aatgatttgc tggtatcggt aaaaaaacta ctggcggaga attatccgga tggtgtgtta
    15961 aggccgggat tcgcctgagt aaacttccct catagtgggg cgtcagacgc ccccttaaac
    16021 attaaaatgt gagcacttta tcggctgaca gcgtccattg cgccagttcc acaagagtac
    16081 cgatttccac cccatcaatc agaggaagtg tgctaatccc gcgcccgtcg gtacaggttt
    16141 tgcacaattt caccggtaca ttctgagcgg taaggatctc cagcatttgc tgaatgttgt
    16201 agccttcccc tggtttttgc ccgcgcaacc cggctgtgac cgcatcagac atcaggaaca
    16261 gacgcagatc cagattgctc tcctgctctc gtaacgcaat ggccagccgc aagctgttaa
    16321 acaaggattc gctcccgtaa ggtgcgccat tggcaacgat cactattttt tgcattattt
    16381 actcctgtat tcagggaatt agacactcat cttctatctt actgcttctg cagcgtctga
    16441 ccaatcggtc acatttttaa ggattttcct gaaagcgcga gaaaatacga caaaagttgc
    16501 cagtaatcgt tattctttaa ggctatggtt tttcattatt accggaagtt accgacgttt
    16561 tgagccgttt cgttcctcgc attatcccgt tttatttact cttgcttgta gcaggcggta
    16621 cagctaacgc acaatctacc ttcgagcaaa aagcggcaaa tccctttgat aataacaatg
    16681 atggtctgcc ggatttaggc atggctcccg aaaatcatga tggggaaaaa cactttgctg
    16741 aaattgtgaa agatttcggc gaaaccagta tgaatgataa cgggctggat actggcgagc
    16801 aggcaaaagc tttcgcattg ggaaaagtcc gcgacgcgct tagtcaacag gttaatcagc
    16861 acgtagagtc ctggctatca ccgtggggaa atgccagtgt tgacgtcaaa gtggataacg
    16921 aaggacattt caccggcagt cgtggaagct ggtttgtgcc gttacaagat aatgatcgtt
    16981 atctcacctg gagccagctt ggtcttactc agcaggataa tgggttggtg agcaatgtgg
    17041 gcgttgggca acgctgggcg cgcggcaact ggctggtggg ttataacact ttttatgaca
    17101 acttgcagga cgaaaatctt cagcgagcgg gctttggtgc cgaagcgtgg ggcgaatatt
    17161 tgcgattatc ggcaaacttt tatcagccgt ttgctgcatg gcatgaacag acagccacgc
    17221 aggaacaacg gatggcgcgc gggtacgacc tgacagctcg gatgcgcatg ccgttctatc
    17281 aacacctcaa taccagtgtc agcctagaac agtattttgg tgatcgtgtt gatttgttta
    17341 actctggtac gggttatcac aatcccgtcg cgttgagtct gggattaaat tacacccctg
    17401 tgccattagt cactgtgacg gcccagcata aacaggggga aagtggcgaa aatcaaaata
    17461 acctcgggct gaatcttaat taccgctttg gtgtaccgct caaaaaacaa ctttctgcgg
    17521 gcgaggttgc cgaaagtcag tcgttacgtg gtagtcgcta tgacaatccg cagcgaaata
    17581 atctaccgac tcttgagtac cgacagcgaa aaacgttaac ggtgtttctg gcgacaccgc
    17641 cgtgggatct aaaacctggc gaaacagtgc cgctgaaatt acaaatccgc agtcgttacg
    17701 gtattcggca actgatttgg cagggcgata cgcagatatt aagtttgacg ccgggcgcac
    17761 aagccaacag cgcggagggc tggacgctga tcatgcctga ctggcagaac ggggaagggg
    17821 caagcaatca ctggcgattg tctgtggtgg tggaagataa ccaggggcag cgtgtctcct
    17881 ccaatgagat cacgctaacg cttgtcgaac cgttcgatgc attgtcaaac gacgaactgc
    17941 gctgggaacc gtaatcagaa aatgcgctcc tgatgcaccc ataccgctgc ttccacgcga
    18001 gacttgagct tcattttctt cagcatgtgc ttgacgtgca cttttactgt gctttcggtg
    18061 atatccaggc ggcgggcaat catcttgttc ggcaaaccct gggcaatcag cttgagaata
    18121 tcgcgctcgc gtggggttaa ctggttaaca tcgcgctcag tagtggcacg gttagcgcgc
    18181 aagctggcgg ccagaacagg cgttaatgct tcgcttaata ccatttcgcc agcagctgcc
    18241 tgatgcaatg ctttcagcag atcttccggt tccatatctt ttaacagata gccatccgcg
    18301 ccgcgtttca gtgcggtgac cacatcttct tcatggttag agacgctgaa taccacaatg
    18361 cgccctgaga gggacttttc gcgcagttta tccagcgttt ccagaccgtt catgccgggc
    18421 atattgagat ctaacaggat cagatcggga tcaagagatt ccgccagttc aataccctgt
    18481 tcgccattac tcgcttcgcc aaccacggtg atatctggtg ccatactgat aagctgtttt
    18541 acgccagttc gcagcatcgg gtgatcgtca atcagcagga tagtagccgg ttcctgatta
    18601 ctcatgggta tctccttgga cgtctgtgaa agttttttcg ggaataaagg tgaccaccac
    18661 ttcggtgcca cctgattcac gacggcggac gcggcaatcg cctcgtaaac tttgcgcacg
    18721 gtcgcgcatt attatcatgc cgtagtgatt gctgcggatg gcattttcag gcacgccaca
    18781 gccgttatcc tggacggtca gtttgacctg attatcgttt tgcgccaccg tcaccacgac
    18841 ctcactcgct tgcgaatgtt tgagggcgtt acttaatgcc tcacgggcaa tttgcaacaa
    18901 gtggattgcc tgatgtgaag gcaccagacg aggcggcaat tgataatcca gcttcaccgg
    18961 gaagccaaat ttggcgctgt actcttcgca actcgcttcc agcgccggac gtaatccagg
    19021 ctcggtgagc tgcaagcgga atgtggtgag caattcacgc aactgcgccc aggatgcatt
    19081 cagttcgtta cggatctgac ttaacagttc gcggctgctt tctggcagcg catcgccctg
    19141 catctgtaaa caactcacct gcatcttcat gcaagagaga gattgggcaa tagaatcatg
    19201 cagttcgcgc gcaatggtgg cacgctcttc catcacgatc aactgttgct gacgttcctg
    19261 atggcgatcc agcgccagcg tggcggtaag ttgttcaacc agggtatcca ccagttgttg
    19321 ttggtcatgg ctaagatgac gcccctgcgg cagggtcgcc agcaaaatac cgtactgcgt
    19381 atgagagtca gccagccgcc acttcagggt cgtgccgcga tcaccaacgg gtaatacgcc
    19441 gcgcgggcag agctggcagc ctttatcatc acaagtcata tctggctggc aggtaaactc
    19501 ctgatgattc tcttcatcat cagtgtcata cacccgcagt tcgatatcac gtagcagggt
    19561 taaattctgt aagccgttga gtaccggtga caggcgttca cacagcgggg cgcgggaatg
    19621 caaacggcgg ttagcctgcc ataaaaaaga gaggatctga tttttatgct ccagcccggc
    19681 ggttttctcc tgaacccgct gctcaagtac ggcataactt tcggccagtt ctgcagacat
    19741 attgttcaac gcagttccaa gcatcgccat ttcgttgcgc ccgctgatgt ttgcgcgttg
    19801 ggtaaaatcg cgatggctga cggcactcgc cattgccagc agttgccgcc acggttgtag
    19861 cagtcgcgcc cgcaaccaga taatagtgaa caccagtaaa agtgccataa ataccgccat
    19921 tacccgatgg accagtacca ctgtctcaat acgcatttcc gtggtgcggt caaaaccaga
    19981 taccagctga tcaagcccgg caacaaactg gctgacatcc gctgacaccg tttctcggtt
    20041 ttgtgcacgc atcagcgcag ggatcagttc attacgccag taatcttgta agccctgtaa
    20101 ttgcgccagt tgtccgtctc gttctgctgc tcgagtcaac tcggcgctaa atgccgtttg
    20161 ttccatctct ttaattaagg gcttgtcttt ctcgcttaat ggcactgccg ccaacagacg
    20221 gtaactttgc atgcgcagcg atcccgcttt gttgatcgca tgggcgctgc cctgaacgcc
    20281 ttgcaccagc cagccagaaa ccgccatccc tgccagtcca atagcagtag aaagcaacac
    20341 aataagcgca acctgattaa ccagggtgag cggagagaga caacgtttaa gcatgtaaac
    20401 cccttccttc aggctttaaa tgagcaataa ccttaatgaa tgtgacgata tattctggaa
    20461 tggcagtatt ctcggctatt ggctgaagta tacccatagc cggaaagagt tactccttat
    20521 ttgccgtgtg gttagtcgct ttacatcggt aagggtaggg attctacagc accgtgaaaa
    20581 atctcataat ttttatgaaa tcactgtact cactatgggt aatgataaat atcaatgata
    20641 gataaagtta tcttatcgtt tgatttacat caaattgcct ttagctccag acactaaggt
    20701 ggcagacatc gaaacgagta tcagaggtgt ctatgagtca ctcatctgcc tccgaaaggg
    20761 ctactggagc tgtcataaca gattggcgac cggaagatcc tgcgttctgg caacaacgcg
    20821 gtcaacgtat tgccagccgc aacctgtgga tttccgttcc ctgtctgctg ctggcgtttt
    20881 gcgtatggat gttgttcagc gctgttgcgg tgaacttacc gaaagtcggt tttaatttta
    20941 cgaccgatca gctatttatg ttgactgcgc tgccttcggt ttctggcgcg ttattacgtg
    21001 ttccatactc ctttatggtt cctatcttcg gtggtcgtcg ctggacggcg ttcagcaccg
    21061 gtattctgat tattccttgc gtctggctgg gttttgccgt gcaggatacc tccacgcctt
    21121 atagcgtctt catcatcatc tctctgctat gcggctttgc tggcgcgaac ttcgcatcca
    21181 gtatggcaaa catcagcttc ttctttccga aacagaagca gggtggcgcg ctgggtctga
    21241 atggtggtct gggaaacatg ggcgtcagcg tcatgcagtt ggttgctccg ctggtggtat
    21301 cactgtcgat tttcgcagta tttggtagcc agggcgtcaa acagccggat gggactgagc
    21361 tgtatctggc gaatgcgtcc tggatatggg tgccgttcct tgccatcttc accattgcgg
    21421 cgtggtttgg catgaacgat cttgctacct cgaaagcctc catcaaggag cagttgccgg
    21481 tactcaaacg gggtcatctg tggattatga gcctgctgta tctggcaacc ttcggctcct
    21541 tcatcggctt ctccgcgggc tttgcgatgc tgtcaaaaac gcagttcccg gatgttcaga
    21601 ttctgcaata cgctttcttc gggccgttta ttggtgcgct ggcgcgttct gcaggtggtg
    21661 cattatctga ccgtctgggc ggaactcgtg tcacgctggt gaactttatt ctgatggcga
    21721 ttttcagcgg cctgctgttc ctgaccttac cgactgacgg gcagggcgga agcttcatgg
    21781 cgttcttcgc ggtcttcctg gcgctgttcc tgacagctgg gctgggtagt ggttccactt
    21841 tccagatgat ttcagtgatc ttccgtaaac tgacaatgga tcgcgtgaaa gcagaagggg
    21901 gttctgacga acgtgcgatg cgtgaagcgg caaccggcac ggcagcggcg ctgggtttca
    21961 tctctgcgat tggcgcgatt ggtggcttct ttatcccgaa agcgtttggt agctcgctgg
    22021 cattaacggg ttcgccagta ggtgcaatga aagtattttt gattttctat atcgcctgcg
    22081 tagtgattac ttgggcggta tatggtcggc attctaaaaa ataaatctta atatttgatt
    22141 atcctttgcg cggcatgatg ccgcgcttt
//
LOCUS       DAZPAB010000051        21913 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZPAB010000051 DAZPAB010000000
VERSION     DAZPAB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.051"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..130)
                     /locus_tag="ACHUPG_001740"
     CDS             complement(<1..130)
                     /locus_tag="ACHUPG_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR0861394.1"
                     /translation="MLARDKSNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVIN"
     gene            complement(140..691)
                     /gene="ybcL"
                     /locus_tag="ACHUPG_001741"
     CDS             complement(140..691)
                     /gene="ybcL"
                     /locus_tag="ACHUPG_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR0861395.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(860..1192)
                     /gene="emrE"
                     /locus_tag="ACHUPG_001742"
     CDS             complement(860..1192)
                     /gene="emrE"
                     /locus_tag="ACHUPG_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR0861396.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(1537..1851)
                     /gene="fliE"
                     /locus_tag="ACHUPG_001743"
     CDS             complement(1537..1851)
                     /gene="fliE"
                     /locus_tag="ACHUPG_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR0861397.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2065..3724
                     /gene="fliF"
                     /locus_tag="ACHUPG_001744"
                     /pseudo
     CDS             2065..3724
                     /gene="fliF"
                     /locus_tag="ACHUPG_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
     gene            3717..4712
                     /gene="fliG"
                     /locus_tag="ACHUPG_001745"
     CDS             3717..4712
                     /gene="fliG"
                     /locus_tag="ACHUPG_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR0861398.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4705..5391
                     /gene="fliH"
                     /locus_tag="ACHUPG_001746"
     CDS             4705..5391
                     /gene="fliH"
                     /locus_tag="ACHUPG_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR0861399.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5391..6764
                     /gene="fliI"
                     /locus_tag="ACHUPG_001747"
     CDS             5391..6764
                     /gene="fliI"
                     /locus_tag="ACHUPG_001747"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR0861400.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6786..7127
                     /gene="fliJ"
                     /locus_tag="ACHUPG_001748"
                     /pseudo
     CDS             6786..7127
                     /gene="fliJ"
                     /locus_tag="ACHUPG_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
     gene            7124..8251
                     /locus_tag="ACHUPG_001749"
     CDS             7124..8251
                     /locus_tag="ACHUPG_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR0861401.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8356..8820
                     /gene="fliL"
                     /locus_tag="ACHUPG_001750"
     CDS             8356..8820
                     /gene="fliL"
                     /locus_tag="ACHUPG_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR0861402.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8825..9829
                     /gene="fliM"
                     /locus_tag="ACHUPG_001751"
     CDS             8825..9829
                     /gene="fliM"
                     /locus_tag="ACHUPG_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR0861403.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9826..10239
                     /gene="fliN"
                     /locus_tag="ACHUPG_001752"
     CDS             9826..10239
                     /gene="fliN"
                     /locus_tag="ACHUPG_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR0861404.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10242..10607
                     /gene="fliO"
                     /locus_tag="ACHUPG_001753"
     CDS             10242..10607
                     /gene="fliO"
                     /locus_tag="ACHUPG_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR0861405.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            10607..11372
                     /gene="fliP"
                     /locus_tag="ACHUPG_001754"
                     /pseudo
     CDS             10607..11372
                     /gene="fliP"
                     /locus_tag="ACHUPG_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            11382..11651
                     /gene="fliQ"
                     /locus_tag="ACHUPG_001755"
     CDS             11382..11651
                     /gene="fliQ"
                     /locus_tag="ACHUPG_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR0861406.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11660..12445
                     /gene="fliR"
                     /locus_tag="ACHUPG_001756"
     CDS             11660..12445
                     /gene="fliR"
                     /locus_tag="ACHUPG_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR0861407.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            12730..13354
                     /gene="rcsA"
                     /locus_tag="ACHUPG_001757"
                     /pseudo
     CDS             12730..13354
                     /gene="rcsA"
                     /locus_tag="ACHUPG_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
     gene            complement(13398..13586)
                     /gene="dsrB"
                     /locus_tag="ACHUPG_001758"
     CDS             complement(13398..13586)
                     /gene="dsrB"
                     /locus_tag="ACHUPG_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR0861408.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13749..13927
                     /locus_tag="ACHUPG_001759"
                     /pseudo
     CDS             13749..13927
                     /locus_tag="ACHUPG_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2525 domain-containing protein"
     gene            14225..15040
                     /locus_tag="ACHUPG_001760"
                     /pseudo
     CDS             14225..15040
                     /locus_tag="ACHUPG_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(15037..16731)
                     /gene="dgcQ"
                     /locus_tag="ACHUPG_001761"
                     /pseudo
     CDS             complement(15037..16731)
                     /gene="dgcQ"
                     /locus_tag="ACHUPG_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
     gene            complement(16902..17084)
                     /locus_tag="ACHUPG_001762"
     CDS             complement(16902..17084)
                     /locus_tag="ACHUPG_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR0861409.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(17163..18080)
                     /locus_tag="ACHUPG_001763"
     CDS             complement(17163..18080)
                     /locus_tag="ACHUPG_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR0861410.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            18253..19173
                     /gene="yedA"
                     /locus_tag="ACHUPG_001764"
     CDS             18253..19173
                     /gene="yedA"
                     /locus_tag="ACHUPG_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFR0861411.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(19162..19632)
                     /gene="vsr"
                     /locus_tag="ACHUPG_001765"
     CDS             complement(19162..19632)
                     /gene="vsr"
                     /locus_tag="ACHUPG_001765"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR0861412.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(19613..21031)
                     /gene="dcm"
                     /locus_tag="ACHUPG_001766"
     CDS             complement(19613..21031)
                     /gene="dcm"
                     /locus_tag="ACHUPG_001766"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR0861413.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(21098..21599)
                     /locus_tag="ACHUPG_001767"
                     /pseudo
     CDS             complement(21098..21599)
                     /locus_tag="ACHUPG_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            complement(21678..>21913)
                     /gene="drpB"
                     /locus_tag="ACHUPG_001768"
     CDS             complement(21678..>21913)
                     /gene="drpB"
                     /locus_tag="ACHUPG_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR0861414.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRSARCRRYE"
BASE COUNT         5367 a         5781 c         5571 g         5194 t
ORIGIN      
        1 aattgataac tttttttccc tgacggatac gacataaagc tggcataaga ggcttaaccc
       61 tgtgaatctc atgatgttta tgcatccgta tttcttcaat ctttaggtta gacttatctc
      121 ttgccagcat actcttaccc tattttatct cataaactgg tgttatctca gcggttgcaa
      181 ttttattggc attaagcata taaccaacta acgctccgct ggaattagaa tccacaggaa
      241 tcttatcagt ttttagagcc catactttaa actggtaatg atggggttta tctcctttgg
      301 gaggacatgc tccaccaaat cctgcataac caaaatcatt tcggccttga acagcaccag
      361 tcggaagttt tgtaccatca cgtcttcctg catcagtggg caaatatgtt acagttgctg
      421 gaatattagc aacagtccag tgccaccatc cactgcctgt aggagcatct ggatcataca
      481 cagttacggc aaagcttttg gtgccttcag gagcaccaga ccaggttaat gagggcgatg
      541 tattaccacc ttcacaccca aatccagaaa atacatgaga cgttgtaagt tgctctcctg
      601 tttttatttc attactagtg acctgaaatg ctgcggcctg cgcagaaaat gttatgaatg
      661 ccaatacact tgaaacgata agttttttca taaaaacctc attgttgtga cctatcgtta
      721 ttttatttga tgtgccttga tctcattatg cataaaggcg caatgatcgc gcaaatgcaa
      781 tcataattat aaaggtgccc cgaacattag ccataatatg cacaaatcag attcctgtta
      841 tctcaaggag aattattttt taatgcggtg cacttcgtga caacaagtta atgactaaca
      901 caccggcaca aatcaacatc atgcctatga tagctggcag gtccagtcgt tgaccaaaaa
      961 atccccagga aagcaaacta atcagaacga taccgactcc tgaccagata gcataagcaa
     1021 tccctgtagg tatataagcc agcgtctgag ctaataacca gaatgatgca caatagcaaa
     1081 taattgtgcc aacagagggc cataaccgtg taaacccctc tgaaaacttc attaaggttg
     1141 taccaatgac ctctgcaagt attgcgccac caagataaat ataagggttc atagcatatt
     1201 ctttcctgtt catactggag agaattgtac tacagtttga acacaactca ccgttttcat
     1261 cctggtgtat gtgtatccaa ggggctctgg cgtggcaaga gataaaggca ggaacaataa
     1321 tgatcagaag gaaccccagg ctaacgaatg agttcactct tcctgggagc aaagcgaaga
     1381 caaaacatgt tatcgcgttt acggatcgtg gatggcagaa aacaaccagg atcaggtatc
     1441 cattttaaac cagaaattga gtgagtttgc cccatccatg ccccacgctg tgggatcgga
     1501 tataattaaa caagcatagt caacaggtta cacaacctac acctgcatgg acatcacttc
     1561 ctgatacgcc gccaccagct tattacgcac ctgaatcccc atttgcatag aaactgaggc
     1621 tttttgcata tcggtcatca catcgtttag cgccacgccg ggttcaccga gggtgaattt
     1681 ttctgcctgc gtgcgggcga ctgtttgtgt atcgcttatg cgatcgagcg cggcgtgcag
     1741 ctgcccggca aaactaatgg tcggttgcgg cagtgattcc tgcgcacgcg cactcatcgc
     1801 cgtagcctgt aactggctga taaccccttc aatcccctgt atcgctgaca ttttcgtctc
     1861 ccggataatt tctggtaaca aagcctacca gtaagtcaat aagacaaagg cgctaaatag
     1921 caacaaaaaa actggtttat tggcggatag aaaaaacgaa agcacaaata atgggagcgt
     1981 caatttttcg agtgtgctga cccgagagtg agtcttgttc cactttgcca ataacgccgt
     2041 ccataatcag ccacgaagtg cgcgatgaat gcgactgcag cccagacaaa atctcttgag
     2101 tggcttaatc gcctgcgtgc gaatccgaaa attccattga ttgttaccgg ttccgcggca
     2161 gtggcggtca tggtcgcact gatcctgtgg gcgaaagccc ccgactaccg cacattattc
     2221 agcaatcttt ccgatcagga tggtggcgca attgtcagcc aactgacgca aatgaatatt
     2281 ccttaccgct tcagcgaagc cagcggcgct attgaggttc cggcagataa agttcacgaa
     2341 ctgcgcctgg cgcaacaagg tttgcccaaa ggcggcgcgg tcggtttcga actgctcgat
     2401 caggaaaagt ttggtatcag ccagttcagc gaacaggtga attatcagcg ggcgctggaa
     2461 ggcgagcttt ctcgtaccat cgaaactatc ggcccggtaa aaggggcgcg cgtacatctg
     2521 gcaatgccga aaccgtcttt attcgtccgt gaacaaaaat ccccttctgc atcggtgacg
     2581 gtaaatctgt tacccggccg cgcactcgat gaagggcaaa ttagcgctat tgtacatctg
     2641 gtttccagcg cggttgctgg tctgccgcct ggaaacgtca cgctggtgga tcagggcggg
     2701 catctgttaa cccagtccaa taccagcggg cgcgatctta atgacgctca gttgaaatat
     2761 gccagcgatg tcgaaggccg tattcagcag cgtattgaag cgatcctgtc acctattgtt
     2821 ggtaacggta atattcacgc ctaggtcacg gcgcaactgg acttcgccag taaagaacaa
     2881 acggaagaac agtatcgccc taacggtgat gaatctcatg cggcgcttcg ttcacgccag
     2941 cttaatgaga gcgagcaaag cggttccggt tatccgggcg gcgtaccggg ggcgttgtcg
     3001 aatcaaccgg cacctgcgaa taacgcgcca atcagcacgc ctccgacaaa tcaaaataac
     3061 cgccagcagc aggcgagcac caccagcaat agtgggccgc gtagcacaca gcggaatgaa
     3121 accagtaact acgaagtcga tcgcaccatt cgtcatacca aaatgaacgt gggcgatgtg
     3181 caacgtctgt cagtcgcggt agtggtgaat tacaaaacct tgccagacgg taaaccattg
     3241 cctctcagca acgaacagat gaagcaaatt gaagctctga cccgcgaggc gatgggcttt
     3301 tctgaaaaac gcggtgattc gctcaatgtt gttaactcgc cgttcaatag cagtgacgaa
     3361 agcggcggag cactgccgtt ttggcaacag caagtgttta tcgatcagtt gctggctgcc
     3421 ggtcgctggt tgctggttgc tggtactgct ggtggcgtgg ttgctgtggc ggaaagcggt
     3481 acgcccgcag ctaacacgtc gcgccgaggc ggtgaagact gtgcagcaac aggcgcaggc
     3541 ccgcgaggaa gtggaagatg cggtggaagt ccgtctgagc aaagacgaac aactccaaca
     3601 acggcgcgct aaccaacgac tgggggcaga agtcatgagc cagcgtatcc gtgaaatgtc
     3661 tgataacgat ccgcgcgtgg tggcgctggt cattcgccag tggataaata acgatcatga
     3721 gtaacctgac aggcaccgat aaaagcgtca tcctgctgat gaccattggc gaagaccggg
     3781 cggcagaggt gttcaagcac ctctcccagc gcgaagtgca aaccctgagc gctgcaatgg
     3841 cgaacgtcac gcagatctcc aacaagcagc taaccgatgt gctggcggag tttgagcaag
     3901 aagctgaaca gtttgctgca ctgaatatca acgccaacga ttatctgcgt tcggtattgg
     3961 tcaaagctct gggtgaagaa cgtgccgcca gcctgctgga agatattctc gaaacccgcg
     4021 ataccgccag cggtattgaa acgctcaact ttatggagcc gcagagcgcc gccgatctga
     4081 ttcgcgatga gcatccgcaa attatcgcca ccattctggt gcatctgagg cgcgcccagg
     4141 ccgccgatat tctggcgttg ttcgatgaac gtctgcgcca cgacgtgatg ttgcgtatcg
     4201 ccacctttgg cggcgtgcag ccagccgcgc tggccgaact gaccgaagta ctgaatggct
     4261 tgctcgacgg tcagaatctc aagcgcagca aaatgggcgg cgtgagaacg gcagccgaaa
     4321 ttatcaacct gatgaaaacg cagcaggaag aagccgttat taccgccgtg cgtgaattcg
     4381 acggcgagct ggcgcagaaa atcatcgacg agatgttcct gttcgagaat ctggtggatg
     4441 tcgatgatcg cagcattcag cgtctgttgc aggaagtgga ttccgaatcg ctgctgatcg
     4501 cgctgaaagg ggccgagcag ccactgcgcg agaaattcct gcgcaatatg tcgcagcgtg
     4561 ccgccgatat tctgcgcgac gatctcgcca agcgtggtcc ggtgcgtctg tcgcaggtcg
     4621 aaaacgaaca gaaagcgatt ctgctgattg tgcgccgtct tgccgaaact ggcgagatgg
     4681 tgattggcag cggcgaggat acctatgtct gataatctgc cgtggaaaac ctggacgccg
     4741 gacgatctcg cgccaccacc ggcagagttt gtgccaatgg tcgagtcgga agaaaccatt
     4801 attgaagagg ccgaacccag ccttgagcag caactggcgc aactgcaaat gcaggcccac
     4861 gagcagggtt atcaggcggg gattgccgaa ggtcgccagc aaggtcacga acagggctat
     4921 caggaaggac tggcccaggg gctggaacaa ggtctggcag aggcgaaggc tcaacaagcg
     4981 ccaattcatg cccggatgca gcaactggtc agcgaatttc aaactaccct tgatgcgctt
     5041 gatagtgtga tagcttcgcg cctgatgcag atggcgctgg aggcagcacg tcaggtgatc
     5101 ggtcagacac caacggtgga taactcggca ctgatcaaac agatccaaca gttgttgcag
     5161 caagaaccgt tattcagcgg gaaaccacag ctgcgcgtac acccggatga tctgcaacgt
     5221 gtggatgata tgctcggcgc aactttaagt ttgcatggct ggcgcttgcg gggcgatccc
     5281 accctccacc ctggcggatg taaagtctcc gccgatgaag gcgatctcga cgccagtgtc
     5341 gccactcgct ggcaagaact ctgccgtctg gcagcaccag gagtggtgta atgaccacgc
     5401 gcctgactcg ctggctaacc acgctggata actttgaagc caaaatggcg cagttgcctg
     5461 cggtacgtcg ctacgggcga ttaacccgcg ctaccgggct ggtgctggaa gccaccggat
     5521 tacaattgcc gctcggcgca acctgtgtca ttgagcgcca gaacggcagc gaaacgcacg
     5581 aagtagaaag cgaagtcgtt ggctttaacg gtcaacggct gtttttaatg ccgctggagg
     5641 aagtggaagg tgtcctgccc ggcgcgcgtg tatatgccaa aaacatttcg gcagaagggc
     5701 tgcaaagcgg caagcagttg ccgctcggtc cggcgttatt aggtcgtgtt ctggacggta
     5761 gcggtaaacc gctcgatggc ctgcccttcc ccgatacgac ggaaaccggt gcgctgatta
     5821 ccccgccatt taacccgttg caacgtacac cgattgaaca tgtgctggac accggcgtgc
     5881 gcccaatcaa tgccctgctt accgtcggac gcgggcagcg tatggggctg tttgccgggt
     5941 cgggcgttgg taaaagtgtg ctgctgggga tgatggcacg ctacacccgc gccgatgtca
     6001 ttgtcgtggg tttgattggt gaacgtgggc gcgaagtaaa agattttatt gagaatatcc
     6061 tcggtgccga aggacgtgca cgctcagtgg tgattgccgc tccggcggat gtttctccgc
     6121 tcctgcgaat gcaaggtgcc gcctatgcca cgcgcattgc cgaagatttt cgcgatcgtg
     6181 gtcagcatgt gttgctgatt atggactccc tcacccgcta cgcgatggcc cagcgtgaaa
     6241 ttgcgctggc gattggcgaa ccacctgcca ctaaaggtta tccaccgtcg gtgtttgcca
     6301 aattaccggc actggtcgag cgtgccggta atggcattag cggcggcggc tcgattaccg
     6361 cgttttatac cgtgctcacc gaaggcgatg accagcagga ccccattgct gactccgcgc
     6421 gggcaatcct cgacggtcac attgtgctgt ctcgccgact ggcggaagcc gggcactatc
     6481 cggctatcga tattgaagcg tcgatcagtc gcgcaatgac ggcgttgatc agtgagcaac
     6541 attacgcgcg agtgcgcacc ttcaaacagc tgttgtcgag ttttcagcgt aaccgcgatc
     6601 tggttagcgt cggcgcgtat gccaaaggca gcgatccgat gctcgataaa gccatcgccc
     6661 tgtggccgca gctggagggc tatttgcaac aaggcatttt tgaacgcgcg gactgggaag
     6721 cgtctctcca ggggctggag cgtattttcc cgacagtgtc ataacccagg agataacggc
     6781 agatggcgga agaacagctc aaaatgttga tcgattatca gaatgaatat cgcaataacc
     6841 tcaatagcga tatgagtgcc gggatgacca gcaaccgctg gatcaactat cagcagttta
     6901 tccagacgct ggaaaaagcc attactcagc atcgccagca acttaatcag tggacgcaga
     6961 aagttgacat tgccctgaac agctggcgag aaaagaaaca acgtttgcag gcctggcaga
     7021 cactgcagga acggcaatcc acggcggcac tgcttgcaga aaaccgcctc gatcagaaaa
     7081 agatggatga gttcgcccag cgcgccgcca tgaggaaacc tgaatgattc gcttagcgcc
     7141 cttgattacc gccgacgttg acaccaccac attgcctggc ggcaaagcca gcgatgctgc
     7201 acaagatttt ctcacgttgt tgagcgaagc attagcaggc gagacaacaa ctgacaaagc
     7261 ggccccccag ttgctggtag caacagataa gcccacgaca aaaggcgagc cgctggtcag
     7321 cgatattctt gccgatgcgc aacaagcgga tttactgatc cctgtggatg aaacacttcc
     7381 tgtcatcaac gacgaacaat ccacatcaac accgttaacc accgctcaga cgatgacgtt
     7441 ggctgcggtg gctgacaaaa atacgacaaa agacgaaaaa gcggatgatc ttaatgaaga
     7501 cgtcaccgca agtctgagcg ccctttttgc gatgttgccg ggttttgaca atacgcccaa
     7561 agtgactgat gcaccgtcaa ccgtgttacc ggcagagaaa ccaacgctct tcacaaaact
     7621 gacttctgcg caactcacta cagcacagcc tgatgacgcc cccggcacac cagctcagcc
     7681 attaacaccg ctggtagcag aagcccagag taaagcggaa gtcatcagca cgccttcgcc
     7741 ggtgaccgct gacgccagcc cgctaatcac tccacaccag acacagccac tgcccaccgt
     7801 cgccgcgcct gttttgagtg caccgctggg ttctcacgaa tggcaacaat cattgagcca
     7861 gcatatttcg ctgttcaccc gccaggggca acaaagtgca gagttgcgtc tgcacccaca
     7921 ggatttaggt gaagtgcaaa tttccctcaa agtggatgat aaccaggcgc agatccagat
     7981 gatttcaccg catcagcacg tacgcgccgc cctggaagca gcgctgccgg tactgcgtac
     8041 gcagctggcc gaaagtggca ttcagttagg gcaaagcaac attagtggcg aaagctttag
     8101 tggtcagcag caggccgctt cccagcaaca gcaaagccaa cgcacagcaa accatgaacc
     8161 tctggcgggg gaagacgacg atacgcttcc ggttcccgtc tctttacaag ggcgtgtaac
     8221 aggtaacagc ggcgttgata ttttcgccta acgtcagagg tagcaccgta atccgcgtct
     8281 tttccccgct ttgttgcgct caagacgcag gataattagc cgataagcag tagcgacaca
     8341 ggaagaccgc aacacatgac tgattacgcg ataagcaaga aaagcaagcg atcgctttgg
     8401 atcccgattc tggtattcat taccctcgcg gcctgtgcca gcgcaggtta cagctactgg
     8461 cattcgcatc aggttgccgc tgacgacaaa gcgcagcaac gcgtcgtgcc ctccccggtc
     8521 ttctatgcgc tggatacctt tacggtcaat ttgggcgatg cggatcgcgt gctttatatc
     8581 ggcataaccc tgcgactgaa agatgaagca acccgctcgc ggctgagtga gtatttgccg
     8641 gaagtccgta gtcgcttgct gttactgttt tcacgtcagg atgctgccgt actggcgaca
     8701 gaagaaggca agaaaaacct gattgccgag attaaaacca cactttccac cccgcttgtt
     8761 gccgggcaac cgaaacagga tgtcaccgac gtgctgtata ccgcttttat tctgcgataa
     8821 cgacatgggc gatagtattc tttctcaagc tgaaattgat gcgctgttga atggtgacag
     8881 cgaagtcaaa gacgaaccga cagccagtat tagcggcgaa agtgacattc gtccgtacga
     8941 tccgaatacc caacgacggg ttgtgcgcga acgtttgcag gcgctggaaa tcattaatga
     9001 gcgctttgcc cgccattttc gtatggggct gttcaacctg ctgcgccgta gcccggacat
     9061 taccgtcggg gccatccgca ttcagccgta ccatgaattt gcccgcaacc tgccggtgcc
     9121 gaccaacctg aacctaatcc acctgaaacc gctacgcggc acagggctgg tagtgttctc
     9181 gccgagtctg gtgtttatcg ccgtggataa cctgtttggc ggcgatggac gcttcccgac
     9241 caaagtggaa ggtcgcgagt ttacccatac tgaacagcgc gtcatcaacc gtatgttgaa
     9301 gctggcgctg gaaggctata gcgacgcctg gaaagcgatt aatccgctgg aagttgagta
     9361 cgtgcgttcg gaaatgcagg tggaatttac caatatcacc acctcgccga acgacattgt
     9421 ggttaacacg ccgttccatg tggagattgg caacctgacc ggcgaattta atatctgcct
     9481 gccattcagc atgatcgagc cgctacggga attgttggtt aacccgccgc tggaaaactc
     9541 gcgtaatgaa gatcagaact ggcgcgataa cctggtgcgc caggtgcagc attcacagct
     9601 ggaactggtg gccaactttg ccgatatctc gctacgcctg tcgcagattt taaaactgaa
     9661 acccggcgac gtcctgccga tagaaaaacc cgatcgcatc atcgcccatg ttgacggcgt
     9721 cccggttctg accagtcagt acggtactct caacggtcag tatgcgttac ggatagaaca
     9781 tttgattaac ccgattttaa attctctgaa cgaggaacag cccaaatgag tgacatgaat
     9841 aatccggccg atgacaacaa cggcgcaatg gacgatctgt gggctgaagc gttgagcgag
     9901 caaaaatcaa ccagcgaaaa aagcgctgcc gacgcggtgt tccagcaatt tggcggtggt
     9961 gatgtcagcg gaacgttgca ggatatcgac ctgattatgg atattccggt caagctgacc
    10021 gtcgagctgg gccgtacgcg gatgaccatc aaagagctgt tgcgtctgac gcaagggtca
    10081 gtcgtggcgc tggacggtct ggcgggcgaa ccgctggata ttctgatcaa cggttattta
    10141 atcgcccagg gcgaagtggt ggttgttgcc gataaatatg gcgtgcggat caccgatatc
    10201 attaccccgt ccgagcggat gcgccgcctg agccgttagt gatgaataac catgctactg
    10261 tgcaatcttc cgcgccggtt tctgctgagc cactgctgca ggtgagcggc gcactcatcg
    10321 ccattattgc cctgatcctc gctgctgcct ggctggtaaa acggctggga tttgccccta
    10381 aacgcactgg cgttaacggt ctgaaaatta gcgccagtgc ttcgctgggc gcgcgtgaac
    10441 gggtcgtggt ggtcgatgtg gaagatgcac ggctggtgct cggcgttacc gcagggcaaa
    10501 tcaatctgct gcataaactt cccccttcag caccaacgga agagataccg cagaccgatt
    10561 ttcagtcggt catgaaaaat ttgcttaagc gtggcgggag atcctgatgc gtcgtttgtt
    10621 ttctgtcgca ccggtccttc tctggctgat tacgcccctc gccttcgcgc aactgccggg
    10681 tatcaccagc cagccgctac ctggcgggta tcaccagcca gccgctacct ggcggtggac
    10741 aaagctggtc gctcccggtg cagacgctgg tgttcatcac ctcgttgacg ttcattccag
    10801 cgattttact gatgatgacc agtttcaccc gcatcattat tgtttttggt ttattgcgta
    10861 acgcgctggg aacgccctcc gcgccaccta accaggtatt gctggggctg gcactgtttt
    10921 tgaccttttt tattatgtcg ccagtgatcg acaaaattta tgtcgatgcg taccagccat
    10981 tcagcgaaga gaaaatctca atgcaggagg cgctggaaaa aggggcgcag ccgctgcgtg
    11041 agtttatgct gcgtcagacc cgcgaggcag atttagggtt gtttgccaga ctggcgaata
    11101 ccggcccgtt gcaggggcct gaagctgtgc cgatgcgcat tttgctcccg gcctacgtga
    11161 ccagcgagtt gaaaaccgca tttcagatag gcttcacgat tttcatccct tttttgatta
    11221 tcgacctggt gattgccagc gtgttgatgg cattggggat gatgatggtt ccccccgcca
    11281 ccattgctct gccctttaaa ctgatgctgt ttgtactggt ggatggctgg caattgctgg
    11341 tcggttcgct ggcgcagagc ttttacagct agagaggcaa aatgacacct gaatcggtca
    11401 tgatgatggg aactgaagcg atgaaagtcg cgctggcact ggcagccccg ctgttgctgg
    11461 tagcgctggt cacgggcctt atcatcagta ttttgcaggc agccacgcag attaacgaaa
    11521 tgacgctgtc gtttattccg aaaatcatcg ccgtatttat cgccatcatt attgccggac
    11581 cgtggatgct caatctgttg ctggattacg tccgcacctt gttcactaac ctgccgtata
    11641 tcatcgggta gcccgtacaa tgatgcagga gacaagcgat caatggcttt cctggttaag
    11701 cctgtacttc tggccgttac tgcgcgtgct ggcgctcatt tctaccgcgc caattctgag
    11761 cgaacgcagc gtgccgaaac gggtaaaact gggtctggca atgatgatca cgttcgccat
    11821 tgccccatca ttacctgcca acgatgttcc tgttttttcg ttctttgctc tgtggctggc
    11881 cgtacagcag atcctgatcg gcattgcgct tggttttacc atgcaatttg cctttgccgc
    11941 tgtgcgaacc gctggcgaaa ttatcggtct gcaaatgggg ctgtcatttg cgacatttgt
    12001 cgatcctggc agccatctca atatgcccgt tttagcgcgc atcatggata tgctggcatt
    12061 actgctgttc ctgacattta acggtcattt atggttgatt tcactgctgg tcgatacctt
    12121 tcacaccctg ccgattggta gcgaaccgtt gaacagcaat gcgtttctgg cacccaccaa
    12181 agcagggagt ttgattttcc ttaacgggct gatgctggcg ttaccgctca ttactctgtt
    12241 gctgaccctg aatctggcat taggtttact gaatcgtatg gccccgcaat tatccatttt
    12301 tgttattgga tttccattaa ctctgactgt cggcatctct ttaatggcgg cattaatgcc
    12361 gttaattgcg cctttttgcg aacatttatt cagtgaaatt tttaatttgc tggctgatat
    12421 tattagtgaa ttaccattaa tataattccg taacgtttat catgctaccc taaggattat
    12481 ccgaaaaata atatctgcga acatcttcca ggatactcct gtagcgaaat atttgtttta
    12541 agctcactca catatcgcaa catttacttt aagacaattc caggcaaatt atacaacact
    12601 ttacgggata gtaagtccgc ctgaaaaatt gcgagagtgg cgcattaggt gacccatgtt
    12661 gttccgttta gtcatgatga aatattcagg taaggggaat tatcgttacg cattgagtga
    12721 gggtatgcca tgtcaacgat tattatggat ttatgtagtt acacccgact aggtttaacc
    12781 gggtatctgt tgagtagagg ggttaaaaaa agagaaatca acgacattga aaccgttgat
    12841 gaccttgcca tagcttgtga ttcacagcgt ccttcagtgg tgtttattaa tgaggactgt
    12901 ttcatccacg atgcttctaa cagtcagcgc atcaagcaca tcattaatca acatcccaat
    12961 acgttattta tcgtttttat ggcaattgcc aatgttcatt ttgatgaata tctattggtc
    13021 agaaaaaaat ttattgatca gttctaaatc gattaaaccg gaatctctcg acgatatcct
    13081 tggcgatatt ctgaaaaaag agacaacgat aacctcgttt ttaaatatgc cgacgttatc
    13141 attgagccga accgaatcga gtatgttgcg aatgtggatg gcaggtcagg gaaccattca
    13201 aatctccgac caaatgaata tcaaagccaa gaccgtttca tcgcataaag gtaatattaa
    13261 acgtaagatc aaaacgcata ataaacaggt tatctaccat gtcgtccgac tgacggataa
    13321 tgtgactaat ggtatttttg tcaacatgcg ctaacacatt ctgactggtg gtttcccacc
    13381 agtcaggctg aataagatta ctctgctttc tcaacaaaga taccgtcctg atgccctgct
    13441 tcattaaaga accagatgcc gagcgggtag tcttccagcg caaccaggta cattgtgcct
    13501 tcactaaact cctcaactgc cagtaccact ccaggacgac gcggaccgcc atccgttttg
    13561 actgttaccc gatcattcac cttcatcgtt ttcctcctgt ggctttgtgc cagtgtagaa
    13621 caatttcgtt tttctggcag cgccaagcgc gcgcgagtgc tgattttctc gacggtctat
    13681 acttaagaga tgccaggcgg acttaacgac tggcggcaac aacagagtaa cggttgcgag
    13741 gaaagatgat gaaaaccgca aaagtgtaca gcgataccgc aaaacgtgag gtcagcgtcg
    13801 atgtcgccgc cagaacgatt ctgaacacgt tagcgtcgat ggacgtgaat atcatacatg
    13861 gcgtgaattg gcggatgcct tcgaactgga tattcatgac ttcagcgtct ctgaagtgaa
    13921 tcgttgaatg cacaataaaa aaatcccgac cctgagggtg tcgggatgaa acttgcttaa
    13981 gcaagaagca cttaaaaaat tcgttacacc aggaaatctg atgtgttcat caccttatct
    14041 gcaatttttt tcgctgacaa gaaaatattc gcgctatgaa tgattaacgc tacttatgaa
    14101 tagaaatgtg acgcgcgtca ccccttggtg gctctggtgt caacgggatc gctcccgaaa
    14161 aaatcatctt tgattatttg tgctgttttg agaatcatcc tggtaagggg agtactgcgc
    14221 gaccatgttt tcaattcaac aaccactact ggtttttagc gatcttgatg gcaccctgct
    14281 ggacagtcat agttatgact ggcaaccggc agccccctgg ctcagccgtt tacacgaagc
    14341 caatattccc gtcattctct gtagcagtaa aactttagcg gaaatgctgt acttgcaaaa
    14401 aatgttgggg ctacaaggct taccgctgat tgcagagaat ggcgcagtga tccagcttgc
    14461 tgaacaatgg caggatatag acggttttcc acgcatcatc tcaggtatta gccatggcga
    14521 aatctgccag gttttaaata cgctacgcga gaaagaacat tttaaattca cgacttttga
    14581 tgatgtcgac gatgcaacca ttgccgaatg gacgggatta agccgtagct aggcggcgct
    14641 gacgcagctg catgaggcgt cggtaacgct aatctggcgc gacagtgacg agcgtatggc
    14701 acaatttatc gctcgtctga acgaactggg cttgcagttt atgcagggtg cgcgcttctg
    14761 gcacgtactg gatgcctctg ccggaaaaga tcaggctgcc aactggatta tcgcgaccta
    14821 tcaacaattg tcaggcagac gcccaaccac acttggcctg ggcgatgggc caaacgatgc
    14881 gcctttactg gaggtaatgg attacgcggt gattgtgaaa gggctaaacc gtgaaggggt
    14941 acatctgcat gatgaggatc cggcccgcgt ctggcgaacg cagcgtgaag gaccggaagg
    15001 ctggcgtgaa gggctggacc attttttctc cgcccgttaa gcgttatcgc tcgcgaatac
    15061 ccgattgcgc ccggcctgtt ttgcgaggta aagccgacgg tcggccaatg actggagttg
    15121 ttcaaaatca taatcaccgg tttcctcgct gctacttacc cccagcgagg cactgatgcg
    15181 tatcgttgta ctcttggcga tcagcatctc tttttcattt aacttcaggc gaatacgttc
    15241 tgcgacctcc gcagcctgcg tcagattcgc gcctggcaga atcacacaaa actcctcacc
    15301 accgacccgc ccggcaacgt cctgcgcacg caaggaactg ctaattaatc cggcagcatg
    15361 agaaagaaca cggtcgcccg cctgatgacc aaagcggtca ttaatcgctt taaaatggtc
    15421 aagatcgacc tgaatgacag aaaaaggatg ttggtgcgtc tgacacaatt tagcgagcgg
    15481 acgggctttt tcgaacagtg cgccacggtt atataaacgc gttaaggtgt cgtgccacgc
    15541 ctgccactgc aacgagcttt gcagaacata catgttgctg accatccggc gaatcacata
    15601 ccaggagagg agtaacatgg tggtaaagag cgcccacagc agggttaatg caatgctgat
    15661 actgccgaaa tcgccgcgca cgccttcgct tagcgtatgg acgcgcgcca gcacaccgtc
    15721 gaaatggtcc agacgttccc agctaacata gcgactgtcc atacgaatgc cgccccgcgt
    15781 gtcatgttcc attgcctgcg ccagcaaggc cagttcacga ggatcaaaaa tattccctgt
    15841 tggatggtca ggattggaag aggtcaaaaa tctcagctta ctgtcataga gctgatactc
    15901 accatcgagg tttttatcga tggcgtttct taaaaattgc tgcatagtac gcacgggaat
    15961 actcatcccc agcacgccat accagtagtt attactgtct accggaacac tgacggtaac
    16021 ctgcggttca gtattgctgg cgtgttccgg ttgcgaagtg aaccagcgta ccgcgcggtg
    16081 acgattttct cgttgcgaat ggccgataaa ccagggttgg gtgacatagc cgtaataacg
    16141 cgttggtaca ttgcgcgtaa acaaggtcgg ctgcgtcgaa acgtaaaatc cagcacgcga
    16201 gacatacatc gcctgttcaa ccatcgacga ggagttgtgc gccagtcgca gcaagtaacc
    16261 aacttccagt gcagcggtaa tttcattgtc gaggctttca ttttcgcgag acaggaaatt
    16321 cccctcgctg actaatgcat ccgatacgcc attgactggc agggtgcgcc gtcggttgag
    16381 ttcgatttgc caggcgtgct cgtcgcgatg ctgttcgaac tcggttacgg cattacgcag
    16441 agaggtgaaa tccagtggcg ccacgagagc ttcgcgcatg ccattacgca aaaagatcag
    16501 tttgtcgacg ttatactgca aatgcttatc gagcgcgttg gctacgtttt ccagatgatt
    16561 acgctagctg gagatatagg catcttccag caccaccact tcacgccagg tgagcaaggt
    16621 ggaaaaaagc aataccacga taaagcagag attaacgaca tgaccgggcc ccaggcggcg
    16681 tgcgagtttt ttcaaccagc tctggttttc catttttgtc tcgtgctgca ccctgacgac
    16741 tcccaacctg cttttttatg attctggcat aacccggcaa tagcgtaccg caaaacagcg
    16801 ataattttat aatggtggca tgataagacg cgactatcat tcattgcagg cattgggaca
    16861 ccaggccgga tgtgatccgg cctggtgtta atgataccct ttcaaacttc ttccgcagaa
    16921 cgactcccct cccccggaac aagatcggtt tcatgaaaag cttcccgctt gaccccgtaa
    16981 ccgtcatacc aacgacactc aaccataccg ctggagtatc cagtgacaat catccgcggg
    17041 ccgccctctt taaccgtaac ttcctcacta accataaagc tcatactcgc ctcctttttt
    17101 cgagtgaaac ttgttcacct tagttgaaga tggcgaattt tgcataatag ccagtgcgag
    17161 tattaatgtg cctgaccgcg cattttcgct actgctttca ctcccagcac cacgatgccg
    17221 ccgatgataa atccaagaat cagatttaaa acagtcggta atatcatcgc caccactgca
    17281 ctttgctgcc cggcgatatg ttcaatagcg tgatgcagcg gcgcaatacc atgtaccaca
    17341 atcccgccgc cgacgaggaa catcgccagc gtgccgacaa tcgataatgc tttcatcagc
    17401 caaggcgcga taatcaataa ccctttacct aatgcctgca tcagcgcgct agatttttcc
    17461 gccagccaat accctaggtc atcaatctta acgataaccc ctaccagacc gtagacgccc
    17521 acggtcacta ccagcgcgat gcctgaaagc accagcacct gattaagcaa cggcgcctct
    17581 gccacaatcc ccagcgtgat ggcgacgatt tccgcagaca aaataaaatc ggtacgaatc
    17641 gcccctttaa ttttgtcctt ttcaaacttc agcggatcct gcgccgccag cttctccaga
    17701 cgctgctggc tctgcgctgg atcttcttta tgtttacgcg cctccagcat atgcagcact
    17761 ttctctaccc cttcaaagca gagaaacgcg ccaccaatca tcaacagtgg cgtaatcgcc
    17821 cacgggataa acgcactgat gatcagcgcc agcggcacca gaatcacttt attgatcagc
    17881 gatcctttcg ccacgcccca gaccacggga agttcccggt tggcccgcac gcctgaaact
    17941 tgttgcgcat tgagcgataa atcatcccct aatacaccgg cggttttctt cgccgccagt
    18001 ttgcccatca cagagatatc gtccagcagt gtggcgatat catcgagcaa cgtcagtaaa
    18061 ctactcccgg ccaaaagcat tccctctcat ttttattgtt gaagggtgta agtatgaagc
    18121 aaaaacggca gctctgatac ttgcttcaca ggtcaacatt tatttaacaa tcaagtgcaa
    18181 ttatatctct cgccagcaga atgattttca cgtttactat gaaccacctt tttatttccg
    18241 ccgtgaggga ttatgcgttt ccgccagttg ttaccgcttt ttggcgcgct gtttgcgttg
    18301 tatatcattt ggggctcaac ctattttgtc attcggattg gcgtgggaag ctggcctccg
    18361 ttaatgatgg cgggcgttcg attccaggca gccggtattt tattgctggc atttttgcta
    18421 ctgcgcggac acaaacttcc cccgctacgt ccgctgctca atgccgcgct gattggcctg
    18481 ttattgctgg ctgtcggtaa tggcatggtg acggttgccg aacatcaaaa tgttccttcc
    18541 ggcatcgccg ccgtagtgat tgcaaccgtg cccctcttta ccctgtgctt cagtcgcctg
    18601 tttggcatta aaacgcgcaa actggaatgg gtgggtattg ccattgggct tgccggaatc
    18661 atcatgctca atagcggtgg aaatttaagc ggcaatccgt ggggcgcgat tctgatttta
    18721 atcggctcga ttagctgggc gtttggctca gtttatggct cgcgcattac cttacctgta
    18781 gggatgatgg cgggtgcgat tgagatgctg gcggcaggcg tggtgttaat gatcgcatcg
    18841 atgattgcgg gtgaaaaact gacggcgctc ccttccctgt caggcttcct tgcggtcggc
    18901 tatctggcgc tgtttggttc gattatcgcc attaacgctt atatgtattt aatccgtaat
    18961 gtcagtccgg ctctcgccac cagctacgcc tacgttaacc cggtggtcgc ggtcttgctg
    19021 ggtacgggac tgggtggcga aacgctgtcg aagattgaat ggctggcgct cggcgtaatt
    19081 gttttcgcgg tggtactggt cacgttggga aaatatctct tcccggccaa acccgttgtc
    19141 gcgccagtta ttcaggatgc atcaagcgag taaatgaatc ccctgcgtgt cgatctgcgc
    19201 gctggcacct tcgccgcaga tccactcttc cagacgctcg gtaagcgccg catccgtcag
    19261 cttctcgcgc ctacgtaacg cacactccca gacaatcagt acgcgccagc cgagctcctg
    19321 caagcgactg atatcgcggc gatcgcgttc aacattttta cctatcttct ccagccagaa
    19381 ctcagttcga gtcgcaggca ctttaaacag atagcagtga tgatgatgcc agaagcagcc
    19441 gtgggtaaat atcacgcagc gatattcatc aacgacaaaa tccgggcgtc cgggcagact
    19501 ggcgtcctga acgcgaaacg caaggccctg cccggttaac aaactggcga ggcgcttctc
    19561 aatcgccgta tcacgcgtgg caatcgcgcg catatttttg ctgcgggtgg ccttatcgtg
    19621 aacgtcgacc atgttgtgcc tcttgctgac gcaacgccac cgcctgtttg atttttggct
    19681 caagcagttt tgccaccgcg gcaaagaccg gcacgaccac cgagttgccg aactggcgat
    19741 aggcctgagt gtccgaaacc ggaatgcgga atttcgcttc tcccggcgct tcaaaaccca
    19801 ttaagcgcgc gcattcacga ggcgttaacc gacgtggacg atgttgctga ttcagcggat
    19861 cgtcaaagtc tttctcaccc gtggccatat cccaaccgcg atcgattaaa atttccgcgc
    19921 catctttgta ataacgagca gacagcgtac gggtgacgct ttgcggattg ttcggataaa
    19981 ccattccata accgaagccg ttaccgcgcg cctgatgttt tttcgcatat cgatagaggt
    20041 acttccacag caccggcgtc aggatatatt tcgcctcaac catcgggtcc agcagctgcg
    20101 ccagcgtcac tcgctgtgca gggaaacatt cgctgatatc acgcagagta aaatcggctt
    20161 tcagattcag atcgcgacga aaacccacca gcacgatgcg ttcacggtgc tgcggcagga
    20221 aatgtttacc atcgatgatt ttcggatcgt ccggcccgtt atcttctgca tcggcaacgt
    20281 catagcccag ttcgtccagc gtctgcatga tgatgcggaa cgtttttccc ttatcgtgac
    20341 tcttcaggtt tttgacgttt tcgagcacaa acatcgccgg acgacgcgcg tcgataatgc
    20401 gcaccacatc aaagaacagc gtgccctggg tatcgcaggc aaaaccgtgc gcccgcccga
    20461 gcgagttctt tttcgatacg ccagccagcg aaaatggctg acaagggaaa ccggccagta
    20521 aaacatcgtg ttcaggaatg tgttgacgaa tatgttccgc cgccgcctca tcactcacgc
    20581 cttcttgatg actgagggtg atgtcgcgga tatcttcatt aaaatgatgc gtcgccgggt
    20641 cgcaataatg gttggcttta taagtgcgta ccgcatgttt gttccattcg ctggtaaaca
    20701 cgcactgtcc accaatcgat tcaaaaccac gacggatgcc gccaattccg gcaaatagat
    20761 cgataaagcg aaacgcataa tgcggatgat gttccggtgg tttgggtaac aacgtttgca
    20821 gatgggcaaa ctctttctca cttaaacggt gccatgccga gtcattcgcc agcgcacgtt
    20881 ttaaaattgc cgcgctccag tgattctcac ccacaccatt aagctgcgcc accagcattt
    20941 taacatcata aatttgcagc agtttctcca gcattgcctg tgcggcattc ccggtgctgt
    21001 atgaatcggt tactgatata ttttcctgca tagatttaac cggccatctg agatggccga
    21061 caggttaaca taattactgc cgtttagggc cacgcgctca gccagcgggt gaaaacgcat
    21121 ctatcacctt gtgatcgata ccttcattct cccccgccag ttcggcaccg agcttcgcca
    21181 tcaaacagcg ccaagctcaa tgcccctgaa acggcaaata cgcgcgccag gccaatcgcc
    21241 cccagggctt ccagccgatc cgcatcctgg acgattttag cttccgttgt taagggggcg
    21301 atttgcgcgc tgaaactgtg agcggcaatg gcatgacaaa cggcctcaat tttctccgcc
    21361 ggaaattgca cgaactcttc acggagcagg cgacgcgtct cttctgccgc caggattgaa
    21421 gaacgctgcc gttgcggatg atttttcgcc aggctcacga tatcgtgaaa ataacaggcg
    21481 gttaaaatca ccagcatatc gacatcgtca tctgccgcca gtttctgtgc cgtagcccag
    21541 acacggcgaa aatgacacag gtcatgcgcc gcgtcctgat gttgatgatg gtttttcaac
    21601 cagttctcga actatgcctg ccagtgttgt aactccatac gcgctccgtt gccaaagagt
    21661 ctcaacctta gcagttttta ttcatagcgt ctgcaacgtg cggaacgagg tcgggtgtac
    21721 catgcaatgc cgcctaacaa ggcaccaacc agggccataa acagaaatcc gaccaatgct
    21781 cctaaccact tccccgcggt agatcctaaa tcactttcaa atcccgacac gggggcatcg
    21841 gggatccggc tcatcaccca gatgtatcgc gccattgccc agacgaaata gccacaccat
    21901 gtatagaaag ccc
//
LOCUS       DAZPAB010000052        21719 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZPAB010000052 DAZPAB010000000
VERSION     DAZPAB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21719
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.052"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..453
                     /gene="tnpA"
                     /locus_tag="ACHUPG_001769"
     CDS             <1..453
                     /gene="tnpA"
                     /locus_tag="ACHUPG_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFR0861415.1"
                     /translation="HCVFLMHVHLVFVTKYRRQIFDHDTTEKLRTYFSNVCADFAAEL
                     VEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRPDIAVRYYYKGVLWSPG
                     YFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFTAHRIKD"
     gene            complement(492..839)
                     /gene="grxD"
                     /locus_tag="ACHUPG_001770"
     CDS             complement(492..839)
                     /gene="grxD"
                     /locus_tag="ACHUPG_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR0861416.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            1174..2001
                     /gene="mepH"
                     /locus_tag="ACHUPG_001771"
     CDS             1174..2001
                     /gene="mepH"
                     /locus_tag="ACHUPG_001771"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR0861417.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            2129..2710
                     /gene="sodB"
                     /locus_tag="ACHUPG_001772"
     CDS             2129..2710
                     /gene="sodB"
                     /locus_tag="ACHUPG_001772"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR0861418.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(2856..4025)
                     /locus_tag="ACHUPG_001773"
     CDS             complement(2856..4025)
                     /locus_tag="ACHUPG_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0861419.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(4191..4280)
                     /gene="cydH"
                     /locus_tag="ACHUPG_001774"
                     /gene_synonym="ynhF"
     CDS             complement(4191..4280)
                     /gene="cydH"
                     /locus_tag="ACHUPG_001774"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR0861420.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            4579..5604
                     /gene="purR"
                     /locus_tag="ACHUPG_001775"
     CDS             4579..5604
                     /gene="purR"
                     /locus_tag="ACHUPG_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR0861421.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(5601..6533)
                     /gene="punR"
                     /locus_tag="ACHUPG_001776"
     CDS             complement(5601..6533)
                     /gene="punR"
                     /locus_tag="ACHUPG_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR0861422.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            6646..7857
                     /gene="punC"
                     /locus_tag="ACHUPG_001777"
     CDS             6646..7857
                     /gene="punC"
                     /locus_tag="ACHUPG_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR0861423.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            8148..9296
                     /gene="cfa"
                     /locus_tag="ACHUPG_001778"
     CDS             8148..9296
                     /gene="cfa"
                     /locus_tag="ACHUPG_001778"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR0861424.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9336..9977)
                     /gene="ribE"
                     /locus_tag="ACHUPG_001779"
     CDS             complement(9336..9977)
                     /gene="ribE"
                     /locus_tag="ACHUPG_001779"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR0861425.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            10192..11565
                     /gene="mdtK"
                     /locus_tag="ACHUPG_001780"
     CDS             10192..11565
                     /gene="mdtK"
                     /locus_tag="ACHUPG_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR0861426.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(11606..12862)
                     /locus_tag="ACHUPG_001781"
     CDS             complement(11606..12862)
                     /locus_tag="ACHUPG_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR0861427.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            13170..13246
                     /locus_tag="ACHUPG_001782"
     tRNA            13170..13246
                     /locus_tag="ACHUPG_001782"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13204..13206,aa:Val,seq:gac)
     gene            13251..13327
                     /locus_tag="ACHUPG_001783"
     tRNA            13251..13327
                     /locus_tag="ACHUPG_001783"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13285..13287,aa:Val,seq:gac)
     gene            13435..13740
                     /locus_tag="ACHUPG_001784"
     CDS             13435..13740
                     /locus_tag="ACHUPG_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR0861428.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            13866..15288
                     /locus_tag="ACHUPG_001785"
                     /pseudo
     CDS             13866..15288
                     /locus_tag="ACHUPG_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(15300..16112)
                     /gene="ydhT"
                     /locus_tag="ACHUPG_001786"
     CDS             complement(15300..16112)
                     /gene="ydhT"
                     /locus_tag="ACHUPG_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR0861429.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(16116..16901)
                     /gene="phsC"
                     /locus_tag="ACHUPG_001787"
     CDS             complement(16116..16901)
                     /gene="phsC"
                     /locus_tag="ACHUPG_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR0861430.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(16898..17566)
                     /locus_tag="ACHUPG_001788"
     CDS             complement(16898..17566)
                     /locus_tag="ACHUPG_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR0861431.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(17630..18268)
                     /locus_tag="ACHUPG_001789"
     CDS             complement(17630..18268)
                     /locus_tag="ACHUPG_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR0861432.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(18281..20382)
                     /locus_tag="ACHUPG_001790"
                     /pseudo
     CDS             complement(18281..20382)
                     /locus_tag="ACHUPG_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(20403..21029)
                     /locus_tag="ACHUPG_001791"
     CDS             complement(20403..21029)
                     /locus_tag="ACHUPG_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR0861433.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(21485..21694)
                     /gene="fumD"
                     /locus_tag="ACHUPG_001792"
     CDS             complement(21485..21694)
                     /gene="fumD"
                     /locus_tag="ACHUPG_001792"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR0861434.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         5429 a         5630 c         5039 g         5621 t
ORIGIN      
        1 cattgtgttt tcctgatgca tgttcacctg gtctttgtca ccaaataccg acgccagatt
       61 tttgaccatg acacgacaga aaaactacgc acttactttt caaatgtatg tgctgatttt
      121 gcagctgaac tggttgaaat ggatggcgaa ccagatcacg tccatttgtt aattaactat
      181 cctcccaaac tggcgatatc cagtctggta aacagcctca aaggcatatc gaataggtta
      241 ctgcgacgag atcgaccaga tattgcagtc aggtattact acaaaggcgt tttgtggagt
      301 cctggctatt ttgccagtag ctgcgaaggt gcgccaatat ccgtcatccg ccagtacatt
      361 gaacaacagc aaacacctgg tcaggtggaa aaccgcgcct tatatccccg ccctgaagga
      421 cgaggtttta cggcgcaccg gataaaggac tgacggatgc ccgttcgcat ccgtcagtat
      481 tgcaggacgg attattccgc gtccggctct tcagacttgt atttagcggc agtttctttg
      541 atcagctgct gcagttcgcc acgctgatac atttcgatca cgatatcaca accgccgacc
      601 agctcgccgt caacccacag ttgcgggaag gtcggccagt tagcatattt cggcagttcc
      661 gcacgaatgt ccggattctg cagaatatca acataggcaa aacgttcgcc acatgcggca
      721 agcgcctgga ctgcctgggc agagaaaccg cagctcggca gtttcggtga acctttcatg
      781 tacagcagga tcgggttttc agcaatctgg cgttggattt tttcgatagt ggtgctcatt
      841 gtcttgcttc ctcaaacttc tttacggcag taatctgaca ttgtagcggg tcagtgcggc
      901 atcggaaaat aacattttca tcacgctttt gctattttat ccctttgctc tatttgttgc
      961 atttcaaata ttcgtttctt ctctgatgcg cggcaaactg gcgtatttat aaccattttt
     1021 agctttcact gctgcatttt tttgcgctcg ccaacgaaac gtatttttta acaataaaag
     1081 ctattaactt tctcttcttc tatgcattag aatcatcaag ttttgtaaat cagacgcagg
     1141 catgatagac ctgccttaac agagggacgc tcagtggcgc ggataaaccg tatttcgatc
     1201 acgctctgtg ctttactttt taccaccctg cctttaacgc ctatggccca tgcttcaaag
     1261 caagccaggg agagttctgc taccactcat atcaccaaaa aagcagataa aaagaaaagc
     1321 acggcaacca ccaaaaagac ccagaaagcc agccagaaaa cagcgaaaaa agccgccagt
     1381 aaaagtatga ccaaaagcaa aaccgcttct tctgttaaaa aatcttccat taccgcttct
     1441 aaaaacgcca aaactcgcag caaacacgcc gtcaataaaa cggcctcagc cagctttacc
     1501 gaaaagtgta ccaaacgtaa gggctataaa tcgcattgtg tgaaagtcaa aaatgccgcg
     1561 tcaggaaccc ttgccgacgc gcacaaagcg aaggtgcaaa aagccacgaa agtggcaatg
     1621 aataaactga tgcagcaaat tggtaagcca tatcgttggg gcggcagctc accgcgtacc
     1681 ggttttgatt gcagcggcct ggtttattac gcttataaag atttggtgaa aattcgtatt
     1741 ccgcgtacgg cgaatgaaat gtatcacctg cgtgatgcag gacctatcga acgtagtgag
     1801 ctgaaaaacg gcgacctggt ctttttccgt actcagggac gcggtacagc cgatcatgtc
     1861 ggcgtgtatg tcggcaacgg caaatttatt cagtcaccgc gcacaggtca ggaaattcaa
     1921 atcacttctc tcagtgaaga ctactggcag cgccactatg ttggcgctcg tcgggtaatg
     1981 accccaaaaa cacttcgcta aaactttacc ctgttgttac ggcaacaggg taagttcatc
     2041 ttttgtctca ccttttaatt tgctacccta tccatacgca caataaggct attgtacgta
     2101 tgcaaattaa taataaagga gagtagcaat gtcattcgaa ttacctgcac taccatatgc
     2161 taaagatgct ctggcaccgc acatttctgc ggaaactatc gagtatcact acggcaagca
     2221 tcatcagact tatgtcacta acctgaacaa cctgattaaa ggtaccgcgt ttgaaggtaa
     2281 atcactggaa gagattattc gcagctctga aggtggcgta ttcaacaacg cagctcaggt
     2341 ctggaaccat actttctact ggaactgcct ggcaccgaac gccggtggcg aaccgactgg
     2401 aaaagtcgct gaagctatcg ccgcatcttt tggcagcttt gccgatttca aagcgcagtt
     2461 tactgatgca gcgatcaaaa actttggttc tggctggacc tggctggtga aaaacagcga
     2521 tggcaaactg gctatcgttt caacctctaa cgcgggtact ccgctgacca ccgatgcgac
     2581 tccgctgctg accgttgatg tctgggaaca cgcttattac atcgactatc gcaatgcacg
     2641 tcctggctat ctggagcact tctgggcgct ggtgaactgg gaattcgtag cgaaaaatct
     2701 cgctgcataa taactgatgg caaatgcagc attgcctgat gcgcttcgct taccatgcca
     2761 acgcggatga ggcgttaaca gcgcattcgg caagataaat cgcacgttgt cagcaactgt
     2821 aacgcagaag gttatccttc tgcgtttttg tttaattagc tgttagcaac gcaaactgtt
     2881 tcaggttgtt ttctggctga cataaacacc agcaataatg ccagtcccgc gacaatcgcc
     2941 cccgtcaccg gcacaaagct gtatcccagc ccagcggaaa ttaccgcacc accagcagct
     3001 gctcccagcg catttccaag attaaaggca ccaatattga ctgatgaaga cagacctggc
     3061 gcttcactgg cgacatgcat cacgcgcatc tgtaacggag gtacaaccgc aaaggttgct
     3121 gcgccccaca ccaccatgct aatagctgcg ccgaactcat tgcgggccag gaacgggatt
     3181 gccagcataa tcaccatcag cagcaacaaa aagcctttca acgtgccgtt aactgaacga
     3241 tctgccagtt tgccgccgag atagttaccg atagagaatc cgacaccaat cagcaccagc
     3301 attgccgtga cgaacaccgg tgttgcgtgg gtaatacttt gcagtaccgg agagatatag
     3361 gtgtagaggg taaacattgc accagctccc agtaccgtcg tcagcaatgc agacagcacc
     3421 tgcggacgca ttaataccgc cagctctttt ttcacttcag gtcgtgcccc tgtaccacct
     3481 ttaggtaatg agaagaacag acttaccatt gaaatcactc ccagccccgc cgttgccaga
     3541 aatgacatcc gccagccgat ggtttcaccc aaccaggtcg ccgccggcac gccaccgata
     3601 tttgccaggg ttaaccccat aaacatagtg gcaactgcgc tggcctgttt atgttttggc
     3661 accacgcttg cggccacgac tgaacccaaa ccaaaaaatg ctccgtgatt caggctggtc
     3721 aaaatacgtg aaagcatcag ggtcatataa tccggcgcga tggcggaaag aacgttgcct
     3781 agcgtgaaaa ttgccatcag gaaaatcaac gcactgcggc gggcacgatg agaaagtaga
     3841 agcgtcatca gcggcgcgcc caccattacg ccaactgcat aggcactgat taacattccg
     3901 gcagcgggaa tcgagacatc cacaccgcgc gcaatgacgg gcaacaagcc cattggcgag
     3961 aactccgttg tcccgatacc aaacgcgcca atcgccagcg ccagcaacgg atagttaatt
     4021 ttcatgcctt atctccacct cttcgcgtca ttacgcgata ttcattaaag tggcgaaagc
     4081 atgacagcaa tcacaaaaaa atgaaaataa caaaaagaga aaacactttt gccattttgc
     4141 taacaaacag gaaggagatg cgagggagaa cgcgctccct cgagaggaaa tcagtgcagc
     4201 gcggcagtca aacccacggc tacgatcaaa ccgaggacga taaccgttgt taccagtgaa
     4261 aatttaaggt cggtgctcat caagttttct ccttttttat taccacacaa aaagtgatat
     4321 tacgcatttt tacacactgt gatgaaaaaa tctcccgtca tttataatga taagtgtttt
     4381 taccacttcc ccttttcgtc aagatcggcc aaaattccac gcttacacta tttgcgtact
     4441 ggccattgac cccttcctga cgctccgtgt cgtttttccg gcgtaccgca acacttttgt
     4501 tgtgcgtaag gtgtgtaaag gcaaacgttt accttgcgat tttgcaggag ctgaagttag
     4561 ggtctggagt gaaatggaat ggcaacaata aaagatgtag cgaaacgagc aaacgtttcc
     4621 actacaactg tgtcacacgt gatcaacaaa acacgtttcg tcgctgaaga aacgcgcaac
     4681 gccgtgtggg cagcgattaa agaattacac tactccccta gcgcggtggc gcgtagcctg
     4741 aaggttaacc acaccaagtc tatcggtttg ctggcgacca gcagcgaagc ggcctatttt
     4801 gccgagatca ttgaagcagt tgaaaaaaat tgcttccaga aaggttacac cctgattctg
     4861 ggtaatgcgt ggaacaatct tgagaaacag cgggcttatc tgtcgatgat ggcgcaaaaa
     4921 cgcgtcgatg gtctgctggt gatgtgttct gagtacccag agccgttgct ggcgatgctg
     4981 gaagagtatc gccatatccc aatggtggtg atggactggg gtgaagcaaa agctgacttc
     5041 accgatgcgg tcattgataa cgcgttcgaa ggcggctaca tggccgggcg ttatctgatt
     5101 gaacgcggtc accgcgaaat cggcgtcatc cccggcccgc tggaacgtaa caccggcgca
     5161 ggccgccttg ccggttttat gaaggcgatg gaagaagcga tgatcaaggt gccggaaagc
     5221 tggattgtgc agggtgactt tgaacctgaa tccggttatc gcgccatgca gcaaatcctg
     5281 tcgcagccgc atcgccctac tgccgtcttc tgtggtggcg atatcatggc aatgggcgca
     5341 ctttgtgctg ctgatgaaat gggcctgcgc gtcccgcagg atgtttcgct gatcggttat
     5401 gataacgtgc gcaacgcgcg ctattttacg ccggcgctga ccacgatcca tcagccaaaa
     5461 gattcgctgg gtgaaacagc gttcaacatg ctgttggatc gtatcgtcaa caaacgtgaa
     5521 gaaccgcagt ccattgaagt gcatccgcgt ttgattgaac gccgctccgt ggctgacggc
     5581 ccgttccgcg actatcgtcg ttaatcaccc gttgcgggag tctcttccgg ctcccgcagc
     5641 cactccttat tcagcgtctc actatcgctg agatactcaa gcagccaggt taacgcaggc
     5701 gacatatcat tttgctgcca tgtcagacaa catgccgaat ccgggaaggg gttttccagt
     5761 tcaagtgcta cccacttccc ctcattaagc cacggtttgg cgaaatgtgt tggcaccatc
     5821 cctatgcata atcctgccga gatacaggtt gccgatgatt cccagtcagg tacgacaagt
     5881 cttttttggt tatccagcaa ccaggtaata cgtttaggta gcgttcgcga ggtgtcttcg
     5941 cgcaccaacg acggccagtt gcgcaacgta tcatcgctga acgggccatc catcaacgcc
     6001 agcgggtggt gactggcaac aacgcaactc cagcttagca tccccatatc ccggaaggca
     6061 taacgaccgc ctaccggaat cgcgcgtgtt gcgccaattg ccagttccac gcgcccgtcg
     6121 gaaagcgcat cccagacacc gttgaacact tcctgaaaga caagaagttc gacatcatca
     6181 aaatggcgat aaaaatcaac gatcatctgc cgtgtacgtt ctggcctgac aatattatcc
     6241 actgcgatag ctaactgacc gcgccagccg ttcgctatct gctgacattg ctggcgggtg
     6301 atctgcattt ttttgacaac agagcgccct tctttgagaa accacgctcc agcagcggtc
     6361 agctccacat cacggtgccg tcgttcaaag agcggtaccg ccagccactc ttccagctga
     6421 cgcacggtat agctgaccgc agaaggaaca cgatgcagct cctgtgccgc agcgctaaaa
     6481 ctaccattac gcgctaccgc atcaacaact tcgagtgaat attctgacca catagtctgc
     6541 ctgcaaaatt tttgaaacca gtcatcaaat attaccgttt cacaacacta atttcactcc
     6601 ctacactttg cggcggtgtt taattgagag atttagagaa tatacatgca acctgggaaa
     6661 agatttttag tctggctggc gggtttgagc gtactcggtt ttctggcaac cgatatgtat
     6721 ctgcctgctt tcgccgccat acaggccgac ctgcaaacgc ctgcgtctgc tgtcagtgcc
     6781 agccttagtc tgttccttgc cggttttgcc gcagcccagc ttctgtgggg gccactctcc
     6841 gaccgttatg gtcgtaaacc ggtgttatta atcggcctga caatttttgc gttaggtagt
     6901 ctggggatgc tgtgggtaga aaacgccgcc acgctgctgg tattgcgttt tgtacaggct
     6961 gtgggggtct gcgccgcggc ggttatctgg caagcattag tgacagatta ttatccttca
     7021 cagaaagtca accgtatttt tgcgaccatc atgccgctgg tgggtctgtc tccggcactg
     7081 gctcccctgt taggaagctg gctgctggtc catttttcct ggcaggcgat tttcgccacc
     7141 ctgtttgcca ttaccgtggt gctgattctg cctattttct ggctcaaacc cacgacgaag
     7201 gcccgtaaca atagtcagga tggtctgacc tttaccgacc tgctacgttc taaaacctat
     7261 cgcggcaacg tgctgatata cgcagcctgt tcagccagtt tttttgcatg gctgaccggt
     7321 tcaccgttca tccttagtga aatgggctac agcccggcag ttattggttt aagttatgtc
     7381 ccgcaaacta tcgcgtttct gattggtggt tatggctgtc gcgccgcact gcagaaatgg
     7441 caaggcaagc agttattacc gtggttgctg gtgctgtttg ctgtcagcgt cattgcgacc
     7501 tgggctgcgg gcttcattag ccatgtgtcg ctggtcgaaa tcctgatccc attctgtgtg
     7561 atggcgattg ccaatggcgc gatctaccct attgttgtcg cccaggcgct gcgtcccttc
     7621 ccacatgcaa ccggtcgcgc cgcagcgttg cagaacactc tgcaactggg tctgtgcttc
     7681 ctcgcaagtc tggtagtttc ctggcttatt agtatcagca cgccattgct caccaccacc
     7741 agcgtgatgt tatcaacagt agtgctggtc gcgctgggtt acatgatgca acgttgtgaa
     7801 gaagttgact gcccgaatca tggcaatgcc gaagtcgctc atagcgaatc acactgatct
     7861 atatcgatat acttatactt aggctgctaa caaaattttg ttgtatgatt gaaattagcg
     7921 gcctatacta atttcgagtt gttaaagcta cgataaatat tatgttttta cggggacagg
     7981 atcgttcccg actcactatg gatggtcatt tcggcaaggg ttcctccttt ccctctgttc
     8041 tacgtcggat tatagactcg cggttttttc tgcgagattt ctcacaaagc ccaaaaagcg
     8101 tctacgctgt tttaaggttc tgatcaccga ccagtgatgg agaaactatg agttcatcgt
     8161 gtatagaaga agtcagtgta ccggatgaca actggtaccg tatcgccaac gaattactta
     8221 gccgtgccgg tatagccatt aacggttctg ccccggcgga tattcgtgtg aaaaaccccg
     8281 atttttttaa acgcgttctg caggaaggct ctttggggtt aggcgaaagt tatatggatg
     8341 gctggtggga atgtgaccga ctggatatgt tttttagcaa agtcttacgc gcaggtctcg
     8401 agaaccaact cccccatcat ttcaaagaca cgctgcgtat tgccggcgct cgtctcttca
     8461 atctgcagag taaaaaacgt gcctggatag tcggcaagga gcattacgat ttgggtaatg
     8521 acttgttcag ccgcatgctt gatcccttca tgcaatattc ctgcgcttac tggaaagatg
     8581 ccgataatct ggaatctgcc cagcaggcga agctcaaaat gatttgtgaa aaattgcagt
     8641 taaaaccagg gatgcgcgta ctggatattg gctgcggctg gggcggacta gcgcactaca
     8701 tggcatctaa ctatgacgta agcgtggtcg gcgtcaccat ttctgccgaa cagcaaaaaa
     8761 tggctcagga acgctgtaaa ggcctggatg tcaccatttt gctgcaagat tatcgtgacc
     8821 tgaacgacca gtttgatcgt attgtttctg tggggatgtt cgagcacgtc ggaccgaaaa
     8881 attacgatac ctattttgcg gtggtggatc gtaatttgaa accggaaggt atattcctgc
     8941 tccatactat cggttcgaaa aaaaccgatc tgaatgttga tccctggatt aataaatata
     9001 tttttccgaa cggttgcctg ccctctgtac gccagattgc tcaatccagc gaaccccact
     9061 ttgtgatgga agactggcat aacttcggtg ctgattacga tactacgttg atggcgtggt
     9121 atgaacgatt cctcgccgca tggccagaaa ttgcggataa ctacagtgaa cgctttaaac
     9181 ggatgtttac ctattatctg aatgcctgcg caggtgcttt ccgcgcccgt gatattcagc
     9241 tctggcaggt ggtgttctca cgcggtgttg aaaacggcct tcgagtcgct cgctaaaggc
     9301 tattctatcg ccccctcttc gggggcgagt tcagatcagg cttctgtacc tggttgattc
     9361 atggcatttt ctcgtgccgc cagcacacgt tctaccgtat ctaccactgc ctgagtttgt
     9421 ggatcgattt caatgttgac gcgtgcgcca agttttttct tcccaagagt cgtgcgttcc
     9481 agtgtttccg gaattaaatg gacgcaaaaa cgcgttggcg tgacttcgcc gacggtcagg
     9541 ctaataccgt cgatgccaat aaatcctttg tacaggatat atttcatcaa ctgactatcc
     9601 tggactttaa accagatctg gcgattattt tccgaggtta atattttcgc cacttcagcg
     9661 gtggtcataa tatgacctga cattaagtgt ccgccaattt catcactgaa tttcgccgca
     9721 cgctcaacgt ttacccaatc ccccactttt aaatcgccaa gattggtaat gcgtaacgtt
     9781 tctttcatca ggtcaaaact gacatggttg ccgttaattt ccgtcacggt caggcagcaa
     9841 ccgttatgcg ccacggaagc accggtttcc aggccgtcca gcatgtggtc gggtaactcc
     9901 accacatgcg tacgaaaatt tggtttctcg tcaatcgaca ccagttttgc ggtgccctgt
     9961 acaatccccg taaacatact tacaactcct gaaatcagtt aagacattct gttcagcaca
    10021 atagcaggtg gaaaacgccc ttaccagtga aggggtaaga atggctattt tttcactgga
    10081 gaattaataa atcctcgcta caatagactg aatttcccct gcttcttctt tttgctgccc
    10141 attcaggcgg ctttttagtc tctcatataa ctacaaataa aaggtgttca cgtgcagaag
    10201 tatatcagtg aagcgcgtct gttattagca ttagcaatcc cggtgattct cgcgcaaatc
    10261 gcccaaactg cgatgggttt tgtcgatacc gtgatggcgg gcggctatag tgccaccgac
    10321 atggcggcgg tcgctatcgg tacttctatc tggcttccgg cgatcctctt tggtcacgga
    10381 ctgctgctgg cattaacgcc ggttatcgcg caattaaatg gttccggtcg acgtgagcgc
    10441 attgcgcatc aggtgcgaca aggtttctgg ctggcaggtt ttgtttccgt tctcattatg
    10501 ctggtgctgt ggaatgcagg ttacattatc cgctacatgg aaaacatcga tccggctctg
    10561 gcggacaaag ccgtgggtta tctgcgtgcg ttgttgtggg gcgcgccggg atatctgttc
    10621 ttccaggttg cccgtaacca atgtgaaggt ctggcaaaaa ccaagccggg tatggtaatg
    10681 ggctttatcg gcctgctggt gaacatcccg gtgaactata tctttattta tggtcatttc
    10741 ggtatgcctg agctcggtgg cgttggttgt ggcgtggcta ctgcggcggt gtattgggtc
    10801 atgttccttg ccatggtttc ttacattaaa cgcgcccgct ccatgcgcga tattcgtaac
    10861 gaaaaaggca ccgctaagcc cgagcctgcg gttatgaaac gactgattca actcggtttg
    10921 ccgattgcgc tggcactgtt ctttgaagtg acactgtttg ccgtcgtggc tctgttagtg
    10981 tctccgctcg gtattgttga tgtcgcagga caccagattg ccctgaactt tagttcactg
    11041 atgtttgtgc ttccaatgtc gctggcggca gcggtaacta tccgcgtagg ttatcgtctg
    11101 ggtcagggct caacgctgga tgcgcaaacc gctgcgcgga ccgggcttat ggtgggtgtc
    11161 tgtatggcaa ccctgacggc cattttcacg gtttcactgc gggagcaaat cgccctgttg
    11221 tacaacgaca atcccgaggt tgtaacgctg gctgcgcatt tgatgttgct ggcggcggta
    11281 tatcagattt ctgactcaat ccaggtgatt ggcagtggga ttttgcgtgg ttataaagat
    11341 acgcgttcca ttttctatat cacctttaca gcttactggg tgctgggctt gccaagcggc
    11401 tatattctgg cactgaccga tctggtcgtt gaacctatgg ggccagcagg cttctggata
    11461 ggctttatta tcggcctgac atcggcagcc attatgatga tgctgcgtat gcggttcctg
    11521 caacgtatgc cgtcagccat cattctgcaa cgagcgtccc gctaataaag acaaggcgca
    11581 accttcacgg gttgcgcctg tgtttttacg caggctggag cgttgcgcca atcccgtctt
    11641 cgtctggctg taatttcaga gcgttacaca gagtttgccg actggcatct gttatctcaa
    11701 cgcgtatatc cccgttaact tcccctttca cattgatgaa gtcaggtgaa atttctttcg
    11761 cactggtttg caggcaaatc gctccctctc cgcgcaggcg tttcactttc agttgcacat
    11821 gcgttgccgc accagtgatc agccacagtt ttccgccatt cagattgatg ttaccaacaa
    11881 tgaaagtgac gctgtcacct tttaccgtac tgccgtcgag aatcaacaca ccgccctgct
    11941 gtacagtaat cgttgattgt tgcagctctg cgccataacc aataatcatt gaagcgccgt
    12001 ccagtaatgc aacactcccg gtcagttcaa ccggtgcgcg atcttcctct acgacaaaat
    12061 tttcgtccgc gctggtgggt gacaacagga tcactgttcc accttgtatt accgcatttt
    12121 ccagcgtacc ggcatgcact tcagcccgcc cccccacatt caccgacagg ttttgcgtct
    12181 taccggaact gtaaagctga aggccatccg tccccagacg ataaatggat ccattctcaa
    12241 caatagtgtc agagtcttca ccaccgagat tattcatggt gccaccagca agcaacgtat
    12301 cactggcttt cccggccagc ataaaaacgc cgccctgatt aatctcacaa ttgctggctt
    12361 ctccacttgg atacaccaac aattcaccac cttcatctac cacgaccgct gaggttgtcc
    12421 cattaaccaa caggccgccc tcttgatcga gaatgatttt ttccgcgcga tgtccttcca
    12481 gtacacgcag gttaccgcca ttttccagca acaaaccgca ggcataaccc tgatcaacgc
    12541 tgaattcacc ttcgggatgg cggccattaa ccgtagcgag cgtagagaga ctaattgcgc
    12601 ctccctcgga ctgaacaata tctgtggcaa tggcaccgtc gctgaccgat agcgtgccat
    12661 tgttaccgta ctgtgtgcat ttggcgatcc caccggttgc cactttcaac cagccagccc
    12721 ccgtgaccac ggtattttcg gctcttccgc cagcttcaac aatcaactcg ccttcagtaa
    12781 gttgcgtcgc gccaaggacc tcgccggtct taacaatttg cacattcccg tcgctcatca
    12841 agtttttcgc atcagatccc atgatttatt cctttgctgc atctgtgtgc ctttattgct
    12901 acctaagtgt aaaggctacg gaggatttat ccacgacaga tttgagatgg tggcaaacaa
    12961 ctctgtttaa actctgatac acgaattatt gggttgtatc agatgtaaat gcgatcctga
    13021 ataaaaatcg cccttgcaaa tcaacaaaat atagccaatt tgcttaaaag ctctgcaaac
    13081 ggtcgaaata gcgcagaaaa ttacgttttg cctcttgcca cctttccact ctgccgctaa
    13141 tattcgtccc cgttgtcacc tacaacgttg cgttcatagc tcagttggtt agagcaccac
    13201 cttgacatgg tgggggtcgt tggttcgagt ccaattgaac gcaccatcct gcgtccgtag
    13261 ctcagttggt tagagcacca ccttgacatg gtgggggtcg gtggttcgag tccactcgga
    13321 cgcaccagat tttcttaatc tggtcttttc ctttttccct ctgtttcttc tctgtatcca
    13381 atacgttaaa agatttacac tgtcttcgta tgcgttatca gaaggagaat cgctatggca
    13441 actttgttac aacttcattt tgcttttaat ggcccatttg gcgacgcaat ggctgagcag
    13501 cttaaaccac ttgctgagtc gattaatcag gaacctggtt ttctgtggaa ggtatggaca
    13561 gaaagtgaaa agaaccacga agccggtggg atctaccttt tcactgatga aaaaagcgcc
    13621 cttgcctatc tggaaaaaca tactgcccga ctgaaaaatc tcggcgttga ggaagttgtc
    13681 gccaaagttt tcgatgtcaa tgagccactt agtcaaatca atcaggcaaa actcgcctga
    13741 cagaatttaa tcaagggcgg ttagcgccct tttcatccct gtctgaaatt tctcaaattc
    13801 taaaaatctc aaccaaactt atctgataac actaaattcg aaagaatgcg tacaggtaag
    13861 taacaatgaa aaaaattgct attgtgggtg ccgggcctac ggggatctac accttattct
    13921 cgcttctaca gcaacaaact ccactttcta tttctatctt cgagcaggct gacgaggccg
    13981 gtgtcgggat gccatacagt gatgaggaaa actcaaaaat gatgctggca aatattgcca
    14041 gtattgaaat accgccgatt aattgtacgt atctcgaatg gctacaaaag caagaagcca
    14101 gccatctcca gcgttatggc gttaaaaaag aaaccttgca cgatcgtcag tttttaccgc
    14161 gaattctgct gggcgaatat ttccgcgatc aatttttacg attagtagac caggcacgac
    14221 agcaaaaatt tgcagtggct gtttatgaat catgccaggt taccgatctg caaattacaa
    14281 atgctggcgt catgctcgct acaaatcagg atttacccag cgagacgttt gatttagcgg
    14341 tgattgccac gggtcacgtc tggcctgatg aagaagaagc aaccagaacg tattttccca
    14401 gcccgtggtc aggcctgatg gaagcaaagg tcgatgcgtg taacgtgggt attatgggaa
    14461 catccttgag cggactggat gcggcaatgg cagtggctat tcagcatggt tcgttcattg
    14521 aagatgataa acaacacgtc gtttttcacc gcgataacgc aagtgaaaag ctaaatatta
    14581 cgttgatgtc gcgcacgggt attttacccg aagccgattt ctattgccct attccctacg
    14641 agcccttaca catcgtcacc gatcaggcat taaatgctga gattcaaaaa ggtgaagagg
    14701 gccttttgga tcgggtattt agattgatag tagaggaaat caagtttgct gatccagact
    14761 ggagccaacg catagcctta gagagcctga atgtcgattc ctttgctcaa gcctggtttg
    14821 ccgagcgcaa acaacgcgac ccatttgact gggcagaaaa aaatctccag gaagtcgaac
    14881 gcaataaacg agaaaaacat actgttccct ggcgttatgt cattctgcgc ctgcatgaag
    14941 ccgtacagga aattgttcct catctgaatg aacacgacca taaacggttc agtaaaggcc
    15001 ttgccagggt tttcatcgat aattatgcgg caatcccttc agagtctatt cgtcgactac
    15061 tggccttacg tgaagcgggg atctgaaatc cctgatgttg gcgaagatta tacgttacag
    15121 caacccgaag atattcgtgg acgcgtagcg ttcggcgcgt tgccctggtt gatgcacgac
    15181 cagcctttcg ttcagggact tacggcatgt gcagaaattg gtgaggcgat ggctcgggcg
    15241 gtcgtaaagc ctgcatcccg tgctcgtcgg cgtctttcgt ttgattaaag gtaaagctat
    15301 cagcactcaa caaactgatg ctgttcatta agacgatagc ggactccggt tcgaatattg
    15361 ttttcacctt caatggcgac ggcgaagcga actctttcgc cgtcatgata tgccagtgcg
    15421 gcgcttcccc ctagcccgag aataattgag tcgaccacgc ccgtgctcac taccgttgaa
    15481 ttttcaccgc tggcaatgat gtgtacattg tcgccgctgt tggcaattct ggcgtttgcg
    15541 ccaaagctgg cgatttgcac caggtcacca ttactggcga catgacaccg ttcacccaat
    15601 gtacaaacgc gtactcgcat tccggtattc gcaatccgac tactatctcc agcgctgctt
    15661 atccgcgaag aattaccggt tacagcaacc cgtgcgcgtt caccggagct gccaatgtga
    15721 ctgttaaagc cgacacttcc aatccgcacg ctataaccca tactggcaat ttgcgcggca
    15781 tacccagcgg ttgcgattcg ggcgttatcc tcacaacagc cgatgcgagc atgatcgtcg
    15841 ctgcgggcaa actgactgca aaatggtatc tctccggggt ctgtggccag tttttgcata
    15901 gaggaaactt cctgatgcgc gaagttttca tccgccagcc attttttcca cgcatactca
    15961 accagactct ccgcccagtc cgtatatcct tcctcaatca gtgcatgacg aatatcggca
    16021 taactaccgc cgcgtgggaa atggcgcagg aaccagcgat acatcaccgc tccgatacgc
    16081 cattctcgca ggtctgctcg tgtaatgatc attcgttagt gccggtgata gccatcgacc
    16141 atgcttttaa aggtttcgtg tggcgtacgc cccgtggtgc aaagataaag atgaccgaag
    16201 ataaaaaaga ggcttataaa tgccagagca aaatgcgcct gcaataacca gtatcttacg
    16261 ccaggaaaca catctcccac ggcttgcgga tagagacaca acagccccgt caatagtaac
    16321 aacggcagca atccatacat gacaccaaca taggcgacct gctgtaaggg attaaattta
    16381 gactgtgttg ttgccgggaa aggatgttct tccccctgca taatgccaaa caaataaaat
    16441 cgcgtttgtt ttgccgctcg ttccagccac ccctgacgac gaatgcgata gtggtgacca
    16501 ttatccccaa cggcattgat cagcacaaag ccgagccagc atgccagtaa caaaaatccg
    16561 caaacttcat gcaccgcaac cagactttta accgcagttg cgcccaccat cgcaaagtga
    16621 tttatcagcc cacttgccag caacaataca aagagtaacg cattcgacca gtgccataat
    16681 ctgaccgctt tgctgtaaag ataaaccttc tcaccatgat cggtggctga tttcttcacg
    16741 ccacgagcac gaagcaaggc gtgcagcccc agcaccaacc acatcccaac cagcaatact
    16801 ccggcaatga tcaaccacac cggccaaaat tcgggagtga actgtggcac atagttcgct
    16861 aactggctct gaaactgttc agcatgttgc gacgggttca tacattttcc tttttaatca
    16921 aatgttgacc gaaccggcga tacagatgcg gttttccggc accaggtagc tgatattgat
    16981 agtatttatt atcctgcaac cacgcctgaa tttctgggct atcttcgcga ccaaagatca
    17041 atgcgtgttc cggacaggcg ctgacgcaaa tgggcggaaa gccttttgcc agtcgggact
    17101 cagcgcagaa atcacattta tctgccactt tagtgacagg gttaaggtag cgaaccaggt
    17161 acggacaagc gccaatgcaa taactgcagc cgatgcactg cgatttttct acccgcacga
    17221 tcccctgctc atcacgccac gatgcccccg tcgggcatac gtcgatgcac ggagcatctt
    17281 cgcagtgctg acatgactgg cgaaaaaagt gatactgcgt ttcgttgtcg ttatcggtga
    17341 ctggaatgtg tgctatcgat aaacggcttc cctgagcggg gacgtggtta gttttacggc
    17401 atgcgcgggc gcaaatatta cagccattgc accgtgactc gtcatgaatc atggcatacc
    17461 gaacttcctt ttcttgcctc gtgttcgcta acagagaaga cgcagaacca gtaaaaaaga
    17521 ttactgttcc catccccaga acaaatttgc gtcgagtgaa ggacatctga ttatccccta
    17581 atgcagtggc tgcaactgtc caccctatcc atgacatctc cctgaaattt caggggttct
    17641 gaaactgctc tacctcgaac cattcgcata agccataagc cttgccatag gtcataaatc
    17701 gctcgctaaa caagtacaac accgccgtcg atgactccac ctgccgtata tcggcatatt
    17761 caggtgcaac atccatcgcg gcgatcgcgg cttcaatctg cgcttcctgg aagtaatatg
    17821 gcgcttgcat cagcatggcg actttgtaag gacgaggata cgtctggcac tcaaaacgaa
    17881 cggcgtgtgc aatggcgcga caaatgtcct gctccacaac ttgcaaagac atcgccgcgt
    17941 agttttcact catggcctgc gttgagtaat agtaatcatc ctgacttccc gtcatacaag
    18001 cgatatccgc gtagtctccg ttctgcttaa tgctttccag caaaactgac agttcctctt
    18061 ctgcaaccga atagggtggc gagagaaaca cggtgcgcgc caccagctgc ccagccgcag
    18121 aatgctggcg aatgaactca gccaggattt cagcaggttc tggttcggtt accgtatcaa
    18181 cggggtgacg catcccctgc aaccattgcc gcttcgcctc gtcaacttcg ctgactttcg
    18241 ctaacggcaa ttcatcctga tggttcatct tacccattcc ttacgcaggc agtagattgt
    18301 gtgctgccag atcggcggca atatcttcca gccccagacg ctgcaatgtt tcgcgggttg
    18361 gacaaccaag ctgtgggtcc cagcccattt ctttgtagaa catcgtgagc gaagcatgca
    18421 tatcgtcacg atccattttg tcagtacctt cagtaaagac cgggatctgc ggatccttgt
    18481 cgaaaaccca ggaacagata agatcgtgtt cattacgcat atctttggtt tgcatcggtt
    18541 ttaccgtgta ggcacgatgc aacgtaaaga tacgctctgc agctaaatct aatttttcct
    18601 gagtcatctc ttcgccggtg atcgctttga agaatttggc ttcaagcgcc agatcgcccc
    18661 ggtaattacg gcttttcagc ggcgaaacgg tcattggcca gacccagttg cacagagtga
    18721 cggcgttatg caaacagacc cgcaacagcg accactttgc atatttaatt tttgcgtcat
    18781 tgattggcgt gtagtttttg gtttcatcgt aagcatcttc agaaccaaaa agttctttcg
    18841 ccacttcacg ttgcagtttc aatggcaagc cggaaccaat aaagttgata tgggtatgcg
    18901 tcatgcagtc acggttgaac atacacttaa caatggaacc gacctgcgct gacgcttcat
    18961 tggcatggtg aaccggatag ccaaacggcg accagagttt atttttcgcg tagccccagt
    19021 actctttacc caaattccag cgttctgcga tggcatatga accatcagcc aggtgactca
    19081 gctcacccac acgatgcgcc agacggtagt aaaaatcttt aatgaagtta acgtcacccg
    19141 cttccagttg atcccagcga atttctgcat actcttcagc tggcagaaca cgcttgaaca
    19201 cacctttgct gtaacaataa gtaaagtcgc gatgcaactg cccgtagtta caccataggc
    19261 cgtagtcatc gaacaaattc agaccaacca ggttaccaat cacacgacca tcgtctttat
    19321 cttcaaaatc tttcggcccg ttcgggaaga tggtggtatg gacaaagttt gctacacaag
    19381 tgttaccacc tgtgctgggc acgccaaact ctttcacccg aggaatattc atttgggtca
    19441 tacaacggat cgggcaagag tggcagccgc tcatttttac tgtgtatttc tctgccgccg
    19501 gtcctaagtc aaaaacggat ttataggtac gaaagccgac cgtattctga ttgcctggcg
    19561 gaatttcacc cgtttcaatc ggaccacctt cagccgcgcc ccaaaacagc cctttacgtg
    19621 ctgtccagcg tgacttggga tctgaatact ctgcccacga ttgtggcgta cttggcacga
    19681 catggttgtt attcgcacca ataagttcag tcatcatgta atcattgaga cgcttcatct
    19741 cctgacgatc ggcaatgttg acccctttcg tcccttcaac cgcaatcgct ttcaggtttt
    19801 tcgaacccat tattgcgcca gttcccgcac cgccgctgtg gttacggcta ttcaacatgc
    19861 cagagagagg aataaggttt tccccagcct gaccaatagc cgccacacag gtttccgggc
    19921 tggtcaatcg acaaatttct tccgtcgttg cgcgcgtccc ttttccccat aagaaatcgg
    19981 ctttttccag gctaactttg tcatctttaa tcttcagcca taccggtgat ttcgccttcc
    20041 cttcgataat aatgacgtcg tatccagcga atttcatttg cgctgcaaaa aagccaccca
    20101 tatgggcatc gcgactaaat ttcctttggt aaaagtagaa agtgaggtga tatttacgcg
    20161 agaactacag ggggcaccag atccaggtaa tgggccggta gcaaagacta atttattcgc
    20221 ttcatcgaaa ggtttcgtgc ctagcggtac ttcgtcatac ataattttgt agccgaagcc
    20281 catgccaccg acaaaacttt taaacttact ggaatcttcg agggtaatat ttcctgtcgt
    20341 gagattgact cttaatatat tacctgtcca accgttagcc atgatttttt cctttgcaag
    20401 attcacacag taatatcttt ccactcgata atttttaacg cccctgttgg gcaggcgttt
    20461 gcgcattcac cgcataacac acatttcgag gattttttac tttcggtatt tacggtggcc
    20521 atcatccacg ggcatgccgt agtacaggcg ctacagccaa tacaacgttt atgatcgacg
    20581 gtaatacagc cttctttctg ctgccaggta atcgcaccaa tcgggcagac gttcatgcat
    20641 tgcggctctt tgcattgacg gcaggtgtcc gcggtatagt tgagatcgcc atacaggccg
    20701 ccgccagagc caaccccgtt gtcgccaaag aaataattgc gatggatttt gatacgggag
    20761 aagaatgtcc ctactgagcc atcgttgaag ttggtacaag agatttcaca gcggtggcaa
    20821 cccgtgcatc gcgctcgctg ggtcaccagc acccctttgg gtgtatttat caaccctact
    20881 gtgccgctat caatatcttc ctgtttgcaa cccaaaagtg acagtaacgc aggggcaatt
    20941 gttagaccag caaggccttt tcctgatata cgcaagaatt ctaatcgcgt taagccaata
    21001 tctaatagtg gacgatcaac cgggttcatt tatttcgcat cctcttcgca aacacagtgc
    21061 tgacaattca gcagcctgat attaagcatt caactaatac gctgctgact ctgtccccaa
    21121 caccagatat tttccctaac ccgatagtgt atgagaaaag atttaacgcc gataaactaa
    21181 tcctttatgg ttaggagaaa aaataatgtg attattctga gcccttaaca ttgatcgtta
    21241 tcaattaaaa ttacaaagca agaaattata agcgaactga tatttattat catttgaaat
    21301 aaatttaact taagcgacaa tcatttgtga cgttcattgc agaaaataaa aataacatta
    21361 tcgctataaa ttaaaataca taccgaaatt cgtataattt cagcagttgt tatttagaat
    21421 gattaactgg accgcaactg aagcaagaat atcaggatga agcttaccgc ctcatcctga
    21481 atggtcattt taccagtgcg ttaatgacgg tttccatcgc ctgcttttcc agctcactgc
    21541 gctgaatgcg tttattggct aatgctgtac gtaatacgcc tgcgataaca atatgctttg
    21601 gttcctgccc cagatcgcgc atttccagca caactttacc gacaactctg cacatttctc
    21661 ggtacagttc atcctctttt gtccgatttc ccattcttta tacctattta tcatgccaa
//
LOCUS       DAZPAB010000053        21512 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZPAB010000053 DAZPAB010000000
VERSION     DAZPAB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.053"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="ACHUPG_001793"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="ACHUPG_001793"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR0861435.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="ACHUPG_001794"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="ACHUPG_001794"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR0861436.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /gene="yjgA"
                     /locus_tag="ACHUPG_001795"
     CDS             complement(2649..3200)
                     /gene="yjgA"
                     /locus_tag="ACHUPG_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR0861437.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="ACHUPG_001796"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="ACHUPG_001796"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR0861438.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5211
                     /gene="cybC"
                     /locus_tag="ACHUPG_001797"
                     /pseudo
     CDS             4829..5211
                     /gene="cybC"
                     /locus_tag="ACHUPG_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="ACHUPG_001798"
     CDS             complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="ACHUPG_001798"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR0861439.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="ACHUPG_001799"
     CDS             complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="ACHUPG_001799"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR0861440.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8410..10065)
                     /gene="treC"
                     /locus_tag="ACHUPG_001800"
     CDS             complement(8410..10065)
                     /gene="treC"
                     /locus_tag="ACHUPG_001800"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR0861441.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10115..11536)
                     /gene="treB"
                     /locus_tag="ACHUPG_001801"
     CDS             complement(10115..11536)
                     /gene="treB"
                     /locus_tag="ACHUPG_001801"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR0861442.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11655..12602)
                     /gene="treR"
                     /locus_tag="ACHUPG_001802"
     CDS             complement(11655..12602)
                     /gene="treR"
                     /locus_tag="ACHUPG_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR0861443.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12787..12840
                     /gene="mgtL"
                     /locus_tag="ACHUPG_001803"
     CDS             12787..12840
                     /gene="mgtL"
                     /locus_tag="ACHUPG_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR0861444.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12981..15677
                     /gene="mgtA"
                     /locus_tag="ACHUPG_001804"
     CDS             12981..15677
                     /gene="mgtA"
                     /locus_tag="ACHUPG_001804"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR0861445.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFRSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="ACHUPG_001805"
     CDS             complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="ACHUPG_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR0861446.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="ACHUPG_001806"
     CDS             complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="ACHUPG_001806"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR0861447.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="ACHUPG_001807"
     CDS             complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="ACHUPG_001807"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR0861448.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="ACHUPG_001808"
     CDS             complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="ACHUPG_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR0861449.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18170..18565)
                     /locus_tag="ACHUPG_001809"
     CDS             complement(18170..18565)
                     /locus_tag="ACHUPG_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR0861450.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="ACHUPG_001810"
     CDS             complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="ACHUPG_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0861451.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19481..20074
                     /locus_tag="ACHUPG_001811"
     CDS             19481..20074
                     /locus_tag="ACHUPG_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR0861452.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20219..20671
                     /gene="tabA"
                     /locus_tag="ACHUPG_001812"
     CDS             20219..20671
                     /gene="tabA"
                     /locus_tag="ACHUPG_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR0861453.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20794..>21512
                     /locus_tag="ACHUPG_001813"
     CDS             20794..>21512
                     /locus_tag="ACHUPG_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR0861454.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKAL"
BASE COUNT         5195 a         5324 c         5669 g         5324 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgctgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaaaagaagc gcaggctgct
     5161 gcagagcaac tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attcctcatt
     5221 tccctgttgc ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga
     5281 ttgctgctgc cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg
     5341 accagcacgt taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta
     5401 tagcctgtcc acacccagat gtctttaccc ggacactcgg cgcggatgcg ttttaccagt
     5461 ttcagaatat ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaatcccc
     5521 tggcgtttga tacgagtgtc attcagatct ttaatgatct tgtcttccat tgctttggta
     5581 aatggctgac cagaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga
     5641 acacacccgg agacaaacag ggtgcagcga gtgccggggc cgttgacgat gtcgacagga
     5701 tagtactgat gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgcatcc
     5761 ggtaatcaat gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat
     5821 gtaggccgga taaggcgttt acgccgcatc cggcatttgc ttagcgcaga gtgaagatta
     5881 acctatctgc ccattcccca aatgtttaac gcggcgctta acttcttcct gcttaccagc
     5941 gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac
     6001 acgggaggcg tcatggttac cacatttcgg gcaagtgaag cctttgctgg tgcactcgaa
     6061 ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg
     6121 tacatgctga tagctgtaat cccagacatc ttccagcgct ttcaggttgt gctgaatgtt
     6181 tggatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag gcgcttcaaa
     6241 gtcgatcttg tcgtacgggt tcaccttctt ctccacatca aggtggaaac tgttggtgta
     6301 gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa
     6361 gcggtcgcac aggttttcac tcggcgtgct gtaaagactg aaaccataac ccgtttcttc
     6421 tttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag
     6481 cttctcgttg tcgtaaacat gctcgccgct gaacagcgcg ttaatggttt cgtggatgcc
     6541 gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc
     6601 agcattcaga cgtacgccac aagcaccttc catatagagg atcggggcga cgcgcgcttt
     6661 cacgccttcg agacgagcga tacgggtcat cagtgcctta cgtgccagca ccagacgttc
     6721 atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag
     6781 gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt
     6841 ttcccacacg ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacgtt
     6901 cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc actccagcgc
     6961 cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc
     7021 aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt
     7081 acgcaggatc gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa
     7141 ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga
     7201 ctggaaggca tcgtagcact ctttgatggt acgagagtta gcgtagcctt cggcgtccgg
     7261 gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtaa caaacggcgc
     7321 cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc
     7381 aataatctgc gcagttaccg cggttgccgt agagatcgac ttcggcggtt caatctcggc
     7441 gttccccatt ttaaaaccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa
     7501 catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc
     7561 ctgcaccacg tcacgcggca gcaggtgctg gcgtgcatag tgtttagcca cgatcccggc
     7621 cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa
     7681 cgaggcgttg gtctgctcga ccagaccacg gatctcctgg ttcaggcgac cgcgtttttc
     7741 acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg
     7801 acccgacatc agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg
     7861 cccctgcatc tgctcgttga caaccgcggc aacagtggcg caataatcgg catcatcgac
     7921 ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac
     7981 tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca
     8041 ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct
     8101 ttccgcaatc gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta
     8161 gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag tctatatata
     8221 gtgctttgca tcaaggatgt tttaactttt tttgatgtag ctcaaagtaa aaagcagagc
     8281 gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc tggttgaaaa
     8341 atcaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt atcggggctt
     8401 agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat tcatggcaca
     8461 gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc cgcgcatttg
     8521 ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca aggtttgccc
     8581 cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat cctggtaatt
     8641 gccccatgtc aggatggctt cttgcttacg cagtgcgatt aacttttggt aggtgtaaaa
     8701 cacagaggag tcatcggcca gcgcggcttc tacgttgatt tgttgatagt tatcgcctag
     8761 gccaatccac ggttcgccag ccgtaaaccc ggcattatcg ccgttgctcc attgcatggg
     8821 cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt cggcatcacg
     8881 accatcgttg cgcagctcgg caaacatatt gaggctctcc acgtcgcgat agtcagtaat
     8941 gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat acggcgttcc
     9001 ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt attcaccttc
     9061 atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca aggcattcca
     9121 tgccacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg ccacaaagtc
     9181 aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt gatgaaaatt
     9241 aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat gctcaaggct
     9301 ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat cgcggttcat
     9361 ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc cgtcgccatc
     9421 caggtcttca gggaaacgcg ggtctttgga gatcagattc accacatcca ggcgcaaccc
     9481 gtcgacccca cgatcggccc agaattcaca gacttttttc agctctgcgc gtaccgctgg
     9541 attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt actgttcgct
     9601 ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt tcggtggcgt
     9661 ttctggttcg ccatcgcgcc agatataaaa ctggcggtaa gggctttctt tgttcagcgc
     9721 ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga gaatgatacg
     9781 aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg tgccgtaggt
     9841 gggatcaatc gccgtatagt tcgctacatc gtaaccgtta tcgacctggg gagagacata
     9901 aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca ggcgttggat
     9961 aacgccacgt aaatcgccgg tgccgctacc cgtggtgtcc tgaaaactct ttggataaat
    10021 ctggtagata acgccgtttt gccaccagtg gggaagatta gtcattacgt tattcctgca
    10081 aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc gtgcccaggc
    10141 ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg gcgatagcca
    10201 ttgccagcgc aaatacctgc cagtagctcg gttgaataga gagaattccc ggcaggcccc
    10261 ctacgccgat accattcgcc ataacgccgt tcaggccgca tagcaatcct gccagaccag
    10321 aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg tacattgcag
    10381 gctcagtgac cccaagccag gcggagatag cggcaggcac ggagatctcg cgttcattgt
    10441 gcttgcggct ggaaatgata atgcctatca cggcggagcc ctgagcgata ttcgacagcg
    10501 caatcagcgg ccacactggc gtgccaccca tgctttgaat catctgcaaa tcaatagcaa
    10561 gggtggtctg gtgtacgccg gtgatcacca gcggcgcgta caggaagcca aacaatgccg
    10621 caccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca acgccatcgc
    10681 caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg aggatcagcg
    10741 aacagacggg caccaccacc agatagaggt aatccggtac gatgcgttta aggcgagttt
    10801 caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc tgatagccca
    10861 ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc ccgagcagat
    10921 aagcgttcat cagctggggg gaaaccagtg tcacgccaag cacgatacca aggatcggcg
    10981 tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag aagaaaatcg
    11041 cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac atttgcgcca
    11101 gcgtctgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg aggatcaaac
    11161 cgccgctaat caacgcgggc agcaacggga agaagatctc cgcgaaatga gagatcaact
    11221 gctcatgcca tttcatattt tgccgggcgg ctttttttac ctgctcttta tcaacctgcg
    11281 cctgtccggt tgacgctatt agtgcttgat agtaatcacc cacgttggtg ccaatcacca
    11341 cctgaaattg cccggcattg gtgaaacagc ctttcaccat ggggagttgc tcaatttctt
    11401 tcggtctggc attggccggt tggttgagga caaagcgcag gcgagtaata cagtggctca
    11461 ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata tccgtttggt
    11521 ttattttgct catcataaag ccccatggca gatgacattt ttggttggct gcagaatata
    11581 ttgcgcggaa aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat cacaatttat
    11641 cgttcaggaa acgatcagga cagggtggcg gggatgatga tttgttgcgg ttcgctgcgc
    11701 ccggttacct gtgcgatcaa ttggcaggcc gcctggcgtc cagcttcggc gtagccagga
    11761 tctacggtga cgatctccgg atgaaggaat ttcattaacg gcgtattacc tacgctcgcc
    11821 agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag agccagcgtg
    11881 tcggttgcgc acagtaacgc ggtagtttca ggcgtaatca cttttgctac gttttcatag
    11941 ccttgcttca tagcaagccc tggcagagcg gcaacgggat gcagtttatg cgctttgcag
    12001 aacgccaggt aggcttcgtg acgtcgcttc ccggtcgtca cgtcactatg cggcacgccg
    12061 agataactga tattgcgatg cccctggtca tagagccgtt gcatcagaat cttgattgcc
    12121 ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag cagaaccagc
    12181 gatgactgcc agtgggctaa catttcttcg gttatgccgg taaaaccgaa cagcactacg
    12241 ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg tggggaaaac
    12301 tgattttcca tcatgattgg atcgtaacct tgttcataga acgctggcag catggtttga
    12361 acggcgagat tttctgacaa cgaatccaga cgggtaacaa tgatggcgac cactttatcg
    12421 ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg attcatcact
    12481 gcttcaacac gctcgcgcgt gcgctgactc acgccgcttt cgttattcag cacccgggaa
    12541 accgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag ccgattttgc
    12601 atcctgttgt cccataacgt gttgtttaat tatttgagcc taactttacc cgtgcattca
    12661 gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta tatcgccata
    12721 aatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc acttaccgga
    12781 ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact tttccggtaa
    12841 gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaat gtccctgctc
    12901 agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt actgtttcag
    12961 acagtgcgga gggactcctt atgtttaaag aaatttttac ccggttcatt cgccatttac
    13021 cttcccgtct ggttcatcgc gatccattgc ctggcgcgca gcagacagtg aatacggcgg
    13081 tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa gaagaattgt
    13141 ggaaaacgtt cgacacccat ccggaaggat taaatcaggc ggaagtggaa tctgcccgcg
    13201 aacaacatgg tgataataaa ttacccgccc aacaaccgtc gccgtggtgg gtacatttat
    13261 gggtctgcta tcgcaacccc tttaatattt tgctcaccat tcttggtgct atttcttacg
    13321 ccacggaaga tttatttgcc gcaggtgtta tcgcgctaat ggtcgctatt tctacgttgc
    13381 tgaactttat tcaggaagct cgttccacca aagcggcaga tgccctgaaa gcgatggtca
    13441 gtaataccgc gacggtactg cgagtaatta acgacaaagg cgaaaatggc tggctggaga
    13501 tcccgataga ccagctggtg cccggcgata ttataaaact ggcggcgggg gatatgatcc
    13561 cggcagattt acgtatcttg caggcgcggg atctgttcgt cgctcaggcg tcgttaaccg
    13621 gtgagtctct gcccgtggaa aaagccgcta ctactcgcca gccggagcac agcaatccgc
    13681 tggagtgcga caccctgtgt tttatgggca ccaccgtggt gagcggcacg gcacaagcaa
    13741 tggtgattgc tacaggagcc aacacctggt ttggtcaact ggcggggcgt gttagtgagc
    13801 aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgttagtatg ctgctgattc
    13861 gctttatgct ggtgatggct ccggtggtgt tgcttatcaa cggttacacc aaaggcgact
    13921 ggtgggaagc ggcactgttt gcgctttcgg tcgcggtagg cttaacgccg gaaatgttgc
    13981 cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa cagaaagtga
    14041 tcgtcaaaca tctggatgcc attcagaact ttggcgcaat ggatattctg tgcactgata
    14101 aaaccggcac cctgacgcag gacaaaattg tgctggagaa tcataccgat atctccggta
    14161 aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattaccag accggactta
    14221 aaaatctgct tgataccgcg gtgctcgaag gtacggatga agagtcagcg cgttcgctgg
    14281 ccagccgttg gcaaaaaatt gatgagattc cgtttgattt cgagcgtcgt cggatgtcgg
    14341 tggtagtggc agaaaatacc gaacaccatc agctggtttg caaaggtgca ttgcaggaaa
    14401 tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtaccgctc gatgacacca
    14461 tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg cgcgtggttg
    14521 cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg gatgaatccg
    14581 acctgatcct cgaaggatat attgctttcc ttgatccgcc aaaagagaca accgctccgg
    14641 cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc gacagtgagt
    14701 tagttgcagc gaaagtgtgc catgaagtgg ggctggatgc gggagaggtg gtcattggta
    14761 gtgatattga aacgctatct gacgatgaac tggcaaatct cgcacagcgc accacgctgt
    14821 ttgcccgcct gacgccgatg cataaagaac gcattgtgac tttactgaag cgcgaagggc
    14881 atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc gctgcggata
    14941 tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat atcatcctgc
    15001 tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc actttcgcca
    15061 acatgctgaa atacatcaaa ttgacggcga gttctaactt cggtaatgtg ttcagcgtgc
    15121 tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg cttattcaga
    15181 acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac gacgagcaaa
    15241 ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatggtc ttcttcggac
    15301 cgatcagctc gattttcgat attctgacgt tttgcttgat gtggtgggta ttccatgcca
    15361 acacgccgga aacgcaaacg ctgttccggt cgggctggtt tgtggtggga ttactgtcgc
    15421 aaacgctgat tgtgcatatg atccgtaccc gccgtgtgcc gtttattcag agctgtgcat
    15481 cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca ttgccgtttt
    15541 caccgctggc cagttatctg cagctgcagg cgctgccgtt aagctatttc ccgtggctgg
    15601 ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaagggttc tatagccgtc
    15661 gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga ttgcagacaa
    15721 agtgagcttt gttcatgccg gatgcggcgt aaacgcccaa tccggcctac aaattcaata
    15781 aattgcagga atcacgtagg cctgataagc gtagcgcatc gggcgatttt gcttttgtca
    15841 tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac gaacagcgat
    15901 tgcttcgatc tcaatcttca cgtctttcgg cagacgggca acttcaacgc aagaacgtgc
    15961 cgggaaagtg gcgttgtgtt cggtgaagaa ggcttcgtaa gtggcgttta cggttgcgaa
    16021 gtcgttcaga tctttcacga acacagtggt tttaacgatg tcgcccactt tcaggccagc
    16081 ggcttcgacg attgctttta cattatccag cgactgacgt gcctgtgcag cgacgtctgc
    16141 cggtacttca ccagttttcg gatttaccgg gatctgaccg gaggtgatga tcatattgcc
    16201 cagatcaacg ccctgtacgt aaggaccgat agctgccggt gcattttccg tcgcgatagt
    16261 tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta tagggagcca
    16321 gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa actctttttc
    16381 acagtatttg catttaagcg cgatgtcatt ggcgcgtttt cgcacggcaa agctggatga
    16441 aaccggttcg gcatggctga tacagttgct gttcgggcag accagcacat tgtcgatgcg
    16501 ctctggcaga cttgggcgcg atttacccac cacttcatag ttgtcgatac ggttaaccgt
    16561 ggcttgcggt gcatacaatg ccagttggtc tacttgctct tcactcaaaa aggtattttc
    16621 gattttgatc agatctttgc ggcccatctc gccagaaggc aaattcagac caatggtgat
    16681 gcgctgatct gtttcggtca acttgaacag actcaacagc ttaaaaccga tctgggcggg
    16741 gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt
    16801 catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg
    16861 gcgagcgaaa atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac
    16921 atccgtcgca atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc
    16981 gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg
    17041 gtccagacgc tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc
    17101 ttcaatagag ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta
    17161 ttgcggcatt gccagcgcat ccggcgcgat aaagtaaaaa cggttgccgt cgaacttcgc
    17221 tagcgcctgg gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac
    17281 gtggagattg tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg
    17341 cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc cagaaaattc
    17401 ggtggctagg cgtgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt
    17461 gctgataacc gaaatggtat cggccagcgt ttcgcctttt ttacccagtg atgtattggc
    17521 gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt cgaaagagag
    17581 gcgggtacgg gtagaggctt cgaagaaaca gctggcaatg actttgtgct tcaacagctc
    17641 tggttgcggg tttgctttca gtttcgccgc tgtcgccagc accagattaa ggtcatcgcg
    17701 actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca tcttttatct
    17761 cctgacacct aggcaaaaaa aagcccctcg attgaggggc tgggaatggg tgatcaacgg
    17821 gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat gtcgaacaca
    17881 ctgaaccata catcctcccg gcaaattgtc cggcattata ctcatagcca gaagcggtgc
    17941 aagcatttga tgcaatattt tgtcagcgca aacggtttat ttgaattaaa agtcaaaata
    18001 tatgcatttt tattttctat gattctggag gggaagtgtc gttcggtatc aataattgca
    18061 gacatttcct gcttttttct ttttcatttt cacgcaatca gaaatgcatt ttattccatt
    18121 catccgactt atagggcgga gggtgaaaac gaacggctaa cactattgct tatagctcag
    18181 ggatacgcgc tatcacttta atttcaaaat caaagcctgc cagccaggta acacccaccg
    18241 ccgtccagtt tggataaggt ggggcgctaa atatttcatt tttcaccgtc atgatgtctt
    18301 cagattgttt ttccggatcg gtatgaaagc tcgtaacatc aatgatatcg tcaaaagtgc
    18361 atcccgcagc tgccagggtc gcatgcaaat tatcaaaggc cagtctgact tgttgctgaa
    18421 aatcgggttc tggtgttccg tcctctcgac ttcctacttg cccggaaaca aacagcaaat
    18481 cgccggaacg aatagccgca gaataacgat gctcagcata cagtgaatgt cggccagcag
    18541 ggaaaacagc ggttctttct accatttggt tatcctcaag atttacgaca tgaacagaag
    18601 atttatattt atcgggagcc gcttttaggg ggacgacgtt agtaaacaaa acccagacat
    18661 catggataat ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat
    18721 ggtatgcatc gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc
    18781 agcgacctct tccggttgcc catgtctttt gatggccata aaactatgca acatatcgcg
    18841 cattggcccg ttggcgggat taacgtcggt atcaattggt cctggctgga cgacgttgat
    18901 ggtgatccct cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt gcagggcaga
    18961 tttactggcg gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc
    19021 gatgattaag atgcgcccgc cttcgggcat ctgccgggcg gcttcaacag aggcatgata
    19081 aggagcatga atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc
    19141 atcgccaaag acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg
    19201 aacgacatca ataacagcgt ctctgtcagc actatctgtg aatactgctg tcgctccagt
    19261 ctcttgtgcc agacgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc
    19321 cccatcggtg acgaaacgac gtacgatagc ggcaccgatt ccacgactgc caccgaggat
    19381 gagaactgtc ttaccggtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc
    19441 attacaaaac tgttttaact ttctgtcaac aggttttgta atggtcacta aaaaacaatc
    19501 tcgcgttcca ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt
    19561 gctttttcac caaaaaggtt tcgatgcagt aagtgtctct gaagttactg attatcttgg
    19621 tattaacccc ccgagtctct acgcggcttt tggcagtaag gctgggttat ttagccgtgt
    19681 actcaatgaa tatgtcggta cggaagctat tccgcttgcc gatattcttc gtgatgatcg
    19741 tccggtaggc gagtgcctgg ttgaggtatt aaaagaagcg gcgcgcagat atagccaaaa
    19801 cggcggctgc gctggctgta tggttcttga aggtattcat agtcatgatc cacaagcgcg
    19861 tgatattgcc attcaatatt atcacgccgc agaaacgacc atttatgact atatcgccag
    19921 gcggcatcca caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct
    19981 ttctgcgaag gcacgggagg ggcactccat cgaacaactc tgtgcaacag ctgccctggc
    20041 aggggaagcg ataaaaactc ttctcaagga gtgatgctgg ccttgatccg aaaggcggga
    20101 acgcgcctgc cgataagttg tgataagaca ataattcacg cgttaaggct ggcggaattg
    20161 atcatctttt cgtataacga tagaaatgaa acattgtttt aattaaggag tggaaaagat
    20221 gatcatcgga aatattcata atcttcagcc gtggctaccc caggagttac gccaggcgat
    20281 tgagcatatc aaagcacacg ttacggcaga aacgccaaag ggcaagcatg atatcgaagg
    20341 caatcgtctg ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc
    20401 ggagtaccat gcccgctatc ttgacattca gattgtgtta aaaggtcagg aaggcatgac
    20461 cttcagcacg caacctgcag gcgcgccgga taccgactgg ttagctgata aagacatcgc
    20521 atttttgccg gaaggcgttg atgagaaaac agttatccta aatgaaggtg attttgttgt
    20581 gttttatccg ggggaagtgc ataaaccgct gtgcgcagtg ggcgcgccag cccaggttcg
    20641 caaagcagta gtgaagatgc tgatggcgtg atgacttttc gccgtaaata actccaggtt
    20701 tacggcgagt ttgtgaaaag agcgtttttt gatatttttt tgtgagtaaa atttgtaatg
    20761 cttagacgtt cttattaact caaggagttc gtcatgggca aaatatcaga tttgaattat
    20821 tctcaacaca ttacattagc cgacaatttt aaacaaaaaa atgaagcctt agatacctgg
    20881 tatgtgggaa tgaatgattt tgcccgaatt gccggggggc agaatagcag aagcaatatt
    20941 ctttctccta gagcattttt ggagtttttg gcaaagatat ttaccctggg ttatgtggat
    21001 tttagcaaac gctccaacga agcgggtaga aatatgatgg ctcatattga gttctcatct
    21061 tattctaaag atactgatgg caatgaaaaa atgaagtttt acatgaataa tcctgaaggg
    21121 gagcgagcgg atttatctaa ggtgaagata gaaattacac ttgcctcagc ttctactaaa
    21181 gggattcgtg aaggacatac agtcattata tttaaacaat ctgatggttc gactaatcgt
    21241 tatgaaggga agtcttttga aagaaaagat gatagttcat tacacctgat tactaacaag
    21301 gttctggcgt gttaccaacg cgaagctaac aagaaaatag cgcgtctatt aaataatcat
    21361 cagaagttaa ataatataca ggagttaaat gattcgcagg agttaaataa ttcacagaag
    21421 ttaaataatt cgcaggagtt aaataactcg caggtgagtt gtaaaggttc agttgattct
    21481 acgattacgg atttattaga aaaagcattg aa
//
LOCUS       DAZPAB010000054        21275 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZPAB010000054 DAZPAB010000000
VERSION     DAZPAB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.054"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..444)
                     /locus_tag="ACHUPG_001814"
     CDS             complement(<1..444)
                     /locus_tag="ACHUPG_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR0861455.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
     gene            complement(499..1350)
                     /gene="manZ"
                     /locus_tag="ACHUPG_001815"
     CDS             complement(499..1350)
                     /gene="manZ"
                     /locus_tag="ACHUPG_001815"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR0861456.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1363..2163)
                     /gene="manY"
                     /locus_tag="ACHUPG_001816"
     CDS             complement(1363..2163)
                     /gene="manY"
                     /locus_tag="ACHUPG_001816"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR0861457.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2226..3197)
                     /gene="manX"
                     /locus_tag="ACHUPG_001817"
     CDS             complement(2226..3197)
                     /gene="manX"
                     /locus_tag="ACHUPG_001817"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR0861458.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3514..3672
                     /gene="yoaL"
                     /locus_tag="ACHUPG_001818"
     CDS             3514..3672
                     /gene="yoaL"
                     /locus_tag="ACHUPG_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR0861459.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            3660..5210
                     /gene="yoaE"
                     /locus_tag="ACHUPG_001819"
     CDS             3660..5210
                     /gene="yoaE"
                     /locus_tag="ACHUPG_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR0861460.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5214..6812)
                     /locus_tag="ACHUPG_001820"
     CDS             complement(5214..6812)
                     /locus_tag="ACHUPG_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR0861461.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="ACHUPG_001821"
     CDS             complement(6943..8307)
                     /gene="sdaA"
                     /locus_tag="ACHUPG_001821"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR0861462.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8491..9069)
                     /locus_tag="ACHUPG_001822"
     CDS             complement(8491..9069)
                     /locus_tag="ACHUPG_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR0861463.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="ACHUPG_001823"
     CDS             complement(9073..10434)
                     /gene="pabB"
                     /locus_tag="ACHUPG_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR0861464.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10508..10687
                     /locus_tag="ACHUPG_001824"
     CDS             10508..10687
                     /locus_tag="ACHUPG_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR0861465.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10807..11106)
                     /locus_tag="ACHUPG_001825"
     CDS             complement(10807..11106)
                     /locus_tag="ACHUPG_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR0861466.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(11528..11872)
                     /locus_tag="ACHUPG_001826"
     CDS             complement(11528..11872)
                     /locus_tag="ACHUPG_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR0861467.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12004..13914
                     /locus_tag="ACHUPG_001827"
     CDS             12004..13914
                     /locus_tag="ACHUPG_001827"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFR0861468.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13972..14667
                     /gene="tsaB"
                     /locus_tag="ACHUPG_001828"
     CDS             13972..14667
                     /gene="tsaB"
                     /locus_tag="ACHUPG_001828"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR0861469.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14707..15288
                     /gene="yeaY"
                     /locus_tag="ACHUPG_001829"
     CDS             14707..15288
                     /gene="yeaY"
                     /locus_tag="ACHUPG_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR0861470.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15494..17179
                     /gene="fadD"
                     /locus_tag="ACHUPG_001830"
     CDS             15494..17179
                     /gene="fadD"
                     /locus_tag="ACHUPG_001830"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR0861471.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17261..18376
                     /gene="rnd"
                     /locus_tag="ACHUPG_001831"
     CDS             17261..18376
                     /gene="rnd"
                     /locus_tag="ACHUPG_001831"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR0861472.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18430..19395)
                     /gene="yeaX"
                     /locus_tag="ACHUPG_001832"
     CDS             complement(18430..19395)
                     /gene="yeaX"
                     /locus_tag="ACHUPG_001832"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR0861473.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19451..20068)
                     /gene="yeaW"
                     /locus_tag="ACHUPG_001833"
                     /pseudo
     CDS             complement(19451..20068)
                     /gene="yeaW"
                     /locus_tag="ACHUPG_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
     gene            complement(20410..21018)
                     /locus_tag="ACHUPG_001834"
     CDS             complement(20410..21018)
                     /locus_tag="ACHUPG_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0861474.1"
                     /translation="MTGEKESKNAERIFMPPEIMRFKERLEEAMNGESSRAFAAKCGL
                     SDGVIRNYLSGKTYPSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDR
                     STLKTKLIAIVELLEDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQ
                     TAKMLESLPTDTRKKILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21188..>21275
                     /locus_tag="ACHUPG_001835"
     CDS             21188..>21275
                     /locus_tag="ACHUPG_001835"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0861475.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT         5265 a         5273 c         5465 g         5272 t
ORIGIN      
        1 gagaagctta taaatttttt ccagatcgtc gatatttcga acgcggatta acagccgacg
       61 ctgttctaat tgcatcacca acacgccatc ttccgacaag ttcatcgctt tgattcggct
      121 atattcaatc cagacattgg cgaagaaaaa acctttttgt ttaaagatga tcttcggaat
      181 gcggatccag aatatataaa aacccatcag agccagtgca cttaataacc atgttgttat
      241 tagcgcgcca tgattcgtga cgttgttata gataagaatg acaatcaatc cgacgaagat
      301 aacgctatcg atgcgaccac gccggagcaa aggaattgcc agcagggtgg ggccgttacg
      361 gcggggcatg atgaactgat cgtagatcgc gaaggccagg agtgcggcga tgaaaagaat
      421 cagtaccagg tccgtgattg tcattaatcc tccagataaa aaaacggggc caaaaggccc
      481 cggtagtgta cgacagtctt acagtcccag caggccgcaa gcgtaaccag cgataccgat
      541 gacgaagaag ccaacgatga tccacagcgg gttaactttt ttgcgcagca gccacataca
      601 agcaaatgtc agcagcagtg gtacaaggcc tggcattaac tggtcaagaa tagtttgcac
      661 ggtggtgaca cgggtctgtc catcctgccc ggtgatggtc gaaaccacca gcgggatgtt
      721 gacatgtgtc cacttgttaa ccaatgcccc catgacaaac aggccgagga tagacgcccc
      781 ttccgtcagt ttttgcagga agccaccacc catatcttta acgatatcga tacctttgga
      841 gtaaccatac gctacgccgt agtaacgggt tgccagacgc accaggttaa acaggatgaa
      901 gaacagcagc ggacctaaca ggctgccgct catcgcgata ccggcaccca gtgctgcaaa
      961 taccggacgt acggttcccc agaagatcgg gtcgcctaca ccagccagcg gccccatcaa
     1021 accgactttg ataccgttga tagcaccgtc atcgatctct gcgccattag cacgctgttc
     1081 ttccagcgcc agggttacgc cgagaatcgg tgcagccacg aacggctggg tgttaaagaa
     1141 ctccaggtga cggcgaatag cttgtttacg agcttcgttg ttctcagggt agaggcgacg
     1201 aattgccggt accatagaga agcagaaacc cagtgcctgc atacgttcga agttccatga
     1261 accctggaag aggttagaac gcaggaatac gccacgaata tcactttgag tgagtttttt
     1321 ctcggtggta gtttgagttg tatcaaccat ttcgctcacc tgttagtcca gttcgttatc
     1381 gagatcgttg ttaccagctg cctgagcagg cgcaccggct acgcggttgt atttcgggct
     1441 aagttggatg tagagcactg ccataacagt accaatcaca cccagagcaa ccaggttaaa
     1501 gttggtgaat gctgcggtta cgaagccgag gtagaagaac ggcatcaggt agccagcacg
     1561 catcatgttg ataaccatcg cataaccaac caccacgatc atgcccccgg cgatattcag
     1621 accattggtc accacttccg gaatcgcatt cagcatgttc tgtacttcgc tggtaccaac
     1681 agacagcgca acgatgacgg ccggaatagc cacacgcatt gcttgcagga acagagaaga
     1741 aacgtggatc caggaaatcg ctgtcaggtt gccgttatca gcagccttat ccgcagcgtg
     1801 ctggaaagca acggtaatag tacgaacgat gatggtcagt acctggcccg cagcggccag
     1861 agggattgcc agtgcgatac ctgcaccaat gctctgatga cctgcgataa ccagaatggt
     1921 agaaatgata gaagccagag cggcgtcagg cgcaactgca gcaccgatgt tcatccagcc
     1981 cagcgcgatc atttccagcg taccaccgat aataataccg gttttcatat ccccaagaac
     2041 gatacccacc agggtacacg cgattagcgg acggtgaaac tgaaattcat cgaggattga
     2101 tcccatacct gcgatacagg ctacgataaa taccagcaca atttgaagag tggtaatctc
     2161 cattgtactt ctcctgttta cgtaagactt aagtgtgaaa actgaatgat aatcaacaca
     2221 atacgttact tatcgatttt gctgatcaga tccatcattt tcagtttcgg atcggtggaa
     2281 accttacgga cttccagctc aataccgcgt gcattcagtt ttttgaacgc ctcgatatct
     2341 ttttcatcaa ccgaaaccgc gttattcacc tgggttttac cctgacggaa tgccatacca
     2401 ccgacgttaa cagaggtgat tttcacgccg ccttcaacga gacgctctac atctgttggg
     2461 ttggtaaata acagcattac gcgttcgcca gcatatttcg ggttgttgta gacgcgaatc
     2521 attttggcaa catcaactac gtgtgctgtt acgcccggag gtgcaacctg ggtgagcagt
     2581 gtcttacgaa cggtatccgc agccacttca tcactaacaa caataatacg ggagacattg
     2641 gtttctttgg tccagcgggt ggcgacctga ccgtgaatca gacggtcgtc gatacgcgca
     2701 aggccaataa ccatgtagtc gtttggcccc attggttttg ccggagttgg agccgctttt
     2761 ggtgctgcgg cagcgggtgc tggcgcggct ttttcaaccg gtttggcttt cagtgctttc
     2821 acgccttcac ggcctgtttc tactgccagc gcaaccagtt catcaaagct cgggtcatca
     2881 tcacgggcca ttaacgtttc cacgagcatt ggaatgttaa cgcctgcaat gacttcataa
     2941 tgctctttgt cgacgacaat gcggctggca gcattgaacg ggctgcctcc ccatgtatca
     3001 acgagaaaca gcacgccttt agtggtgtcg agttttgcca actgagcgtt gtatttttca
     3061 atcagcgttt cggcattttc acctggaacg aaatcgatcc agccgacgtt ttcctgctcg
     3121 cctaacagca tttctgccgt tttaagcaac tgctctgcag cccaaccatg tgtgcctata
     3181 acaatagcaa tggtcacttg ctacctcctt tattatcgtt aacacctcaa cgtgccagat
     3241 gtatttttga atcgtcgtcc acaatcgaat cgattcagat aagggctaca accaaaaacc
     3301 cctgcgtttc gcgatttatt ttagatatcg aaaaaattat tttatgtgat gaagatccgt
     3361 aatttaactt tcgattatca gaaatttcga aaggtaaaat atccttgtca cattcgtttg
     3421 caaaggaagg taaatctttg ccaaatcaga ggcgtctctg atatgtttaa ctcccgttta
     3481 acaaccatgg agtatagggc agtagcccgc agtatggatc gtcaccgacg tcatttcagc
     3541 atcaggcctt ttaacgcctg cctttctggc actctatgcc gcacctttcg tttgcatttt
     3601 gtcgttacgc ctgcattatt tctggcgtcg aatagctatt ccttaagcag gagcttgtca
     3661 tggaattctt aatggacccc tcaatttggg cggggctact cacgcttgtt gttctcgaaa
     3721 ttgtgctggg tatcgataac ctggtcttca tcgccattct tgctgacaaa ctgccgccaa
     3781 aacaacgcga taaagcgcgt ttgctggggt tatcactggc gctgattatg cgtctggggc
     3841 tgctgtcgct gatttcatgg atggtcacgc tgaccaaacc gctatttacc gtcatggatt
     3901 tctccttctc cggacgcgat ctgattatgt tgttcggggg gatattcttg ctgttcaaag
     3961 caacaaccga actgcatgaa cggctggaaa accgcgatca tgattccggc cacggtaaag
     4021 gctacgccag tttctgggtg gtcgtcacac agattgtcat ccttgacgcc gtcttctcgt
     4081 tggatgcggt aattactgca gtagggatgg ttaaccatct gccggtgatg atggcggcgg
     4141 tagtgattgc gatggcggtt atgttgctgg catcgaaacc gctgacgcga ttcgttaacc
     4201 agcaccccac ggtggtggtg ctctgtctga gcttcctgtt aatgattggt ctgagtctgg
     4261 tggcagaagg tttcggtttc cacattccga aaggttacct gtatgccgcg attggcttct
     4321 cgatcattat cgaagtgttt aaccagattg cgcgtcgcaa ctttattcgt caccagtcga
     4381 ctttgccgct gcgagcgcgt actgccgatg ccatcctgcg tttgatgggc gggaaacgtc
     4441 aggccaatgt tcagcacgat gccgataacc cgatgccgat cccggaaggt gcatttgccg
     4501 aagaagaacg gtacatgatt aacggcgtac tgacgctggc gtcgcgttct ctgcgcggga
     4561 tcatgacgcc gcgcggtgaa ataagctggg ttgacgctaa tctcggggtc gatgaaatcc
     4621 gcgagcaact gctctcttca ccgcacagtc tgttcccggt atgtcgcggt gaactggatg
     4681 aaatcatcgg tatcgtacgt gctaaagaac tgctggtggc gctggaagag ggcgttgatg
     4741 tggcggcgat tgcttcggcg tctccggcga ttatcgtccc ggaaaccctc gatccgatca
     4801 acctgctggg cgtgctgcgt cgcgctcgcg ggagctttgt tatcgtgacc aacgagtttg
     4861 gtgtggtaca aggcttggtc acgccgctgg atgtgctgga agccattgcg ggtgaattcc
     4921 cggacgctga cgaaacgccg gaaatcatta ccgatggtaa cggctggctg gtaaaaggcg
     4981 gtacagattt gcatgccttg cagcaggcgc ttgatgttga gcaccttgcc gatgacgatg
     5041 atatcgcgac ggtcgcgggc ctcgtgatct cggcaaatgg tcacattccc cgtgtgggcg
     5101 atgtgattga tgtagggcca ctgcatatca ccatctttga agccaatgat tatcgtgttg
     5161 atctggttcg cattgttaaa gagcaaccgg cgcacgatga agatgagtaa gcgttaacgt
     5221 aacgacataa tgggcgtgat atgtccatta tgccgggcgg gcggcggttg gctgcccgcc
     5281 agccatttgg gaaaatcacg tagcggcatc ggctgtgcgt acaaataccc ttgcaaaata
     5341 tgcacgccat gacggcgcaa atacttcgcc tgttcttgcg tttccacgcc ctccgccacc
     5401 agttcaatat tcagtctttg ccccagcgcg atgatgatat cggtcaccgt cgagttaacc
     5461 gcgtcagaac ctatagctgc ggtaaatgac ttatcaattt tcagcacgtc aggacgtaat
     5521 gtttcaagcc aggaaaacga gctgttgccg gtgccgaagt catcaatcgc cagtttgacg
     5581 tttttacgat gcagctcgcg ggcaatccga taatcaacat ccagtaaggc atcgcgttcg
     5641 gtgatttcga ggatcagttg ctgaatcggg tgagcgctaa accagtactg attgagatct
     5701 tttatcagca caccacggcg aaaatggctg ggtgcgacgt taatgccaac atgaaactga
     5761 ctactcatcg ggaaaacatg gtgctgacga atggtttctg ccatcacata gtgggtcagt
     5821 ggcacaatta aatgatgttc ttccgcgata ggaataaaca catccggtga aatccagccc
     5881 tgacgcggat tattccagcg cagcaaaatc tctacaccaa tacattgctg gctgcgcgca
     5941 ttaagcaaag gctgacagaa caattcgaac tcatgttgcg ccaggcccag attaatttcg
     6001 cgggaaaagc tcatccggta agcggtcgcc agccaggcga tatagcccac cagtaaactt
     6061 agcagcaccg ccagcggtaa ttgtgttgga agatagtgcc atgccagcgc cgtagccccc
     6121 ggaccattta cgttaatggt aaaaggaaag ctttgcgaag aaacctgata gttttcattg
     6181 tcttcaggtt gcggaaggga atctaccagc ccattaccat agagcaaatg ccgtttgtcc
     6241 accgtcaggc tggcgctact gatttgcggc agttgtggct caaggagcat tgccgtcagt
     6301 aagtcgatgt taatcatctc tatgacccca gcattgctac tgcccgctgc aggcgtccat
     6361 tgaatcaaaa ccggactgcc tttaatcagg gcacggtcga tcgtcaggcg taaaagtggt
     6421 tgcggtgcag gaagttcagc cagaatgtcc acgacgggca cattgcgata accaaaaatg
     6481 ctggagcaat aaagtgtgcc gtcttgcacc aggccaatgg atcgcacagt ttggagtttt
     6541 gccgcctgtt tacgtaatgg caaatgggcg acagagcagg gcagaccaat cagcggaagc
     6601 aagactttgc tacctgcctg taggggaagc agtactttat ctaattcctc tacagcgtga
     6661 ttggcgaatt gtactacccg ttgttgattt aagttacgct gtgaaataaa tcgcacgctc
     6721 agggtcgaag cgagcgtaac gagtgcgcaa atcgtacaaa caatcattcg attacggcga
     6781 taggttttaa tgatccgttg tgctttttgc atgcgggcca ccctgtaagc gtatggcgac
     6841 aaacgcctga cgcaacagtg gaagtgtagt ggggaatatt acagcagacg agaaagcggg
     6901 tataaattcg cccatccgtt gcagatgggc gagtaagaag tattagtcac actggacttt
     6961 gattgccaga ccaccgcgtg aggtttcgcg gtatttggcg ttcatgtcct taccggtttc
     7021 gtacatcgtt tcgatgacct tatccagcga gacgcgcggt gcactggtgc ggcgcagagc
     7081 catccgtgcg gcgttaatcg ccttcacaga ggcaatggca ttacgctcaa tgcacggcac
     7141 ctgaacctgc cctgcaaccg ggtcgcaggt taaaccaagg ttgtgttcca tgccaatttc
     7201 cgccgccacg caaacctgtt ccgggctacc gcccagcagt tctgcaagac ccgcagcagc
     7261 cattgaacag gcaacaccca cttcgccctg gcaaccaact tccgcaccgg aaatagaggc
     7321 gttcatttta tacaatgcac caatcgcgcc cgctgccata aagtaacggg tatagatgtc
     7381 tgggctgacc gattcaataa agtggtcata gtaagccagc actgccggaa cgataccgca
     7441 ggcaccgttg gttggcgcag ttaccacacg accaccggcg gcgttttctt cgttaactgc
     7501 cagcgcaaac atgtttaccc agtcaatgac attcatcgga tcgttagaca gtttatcgct
     7561 ggaaactaac atccggcgca gggcagacgc acgacgtggc acgcgcagcg ggcctggcag
     7621 tacaccttcg gtgttcatcc cgcgatcgat acatgcctgc atggtttgcc agacatgcgc
     7681 gaaatactcg tcgatctctt tcttgctgtg cagcgccagt tcgttctgca tagcgagacc
     7741 agacagcgaa tagccggttt cattacagta cgcgagcagt tcggtggcag atttgaacgg
     7801 atacggcacg ctcacttcgt tggcagcatc ctgaccaaag tgttcttcat cgacgataaa
     7861 accgccgccg atggaataat aagttttgct gtagacgact tcatcgccgt tataggcgtg
     7921 gatttgcata ccgttttcat gcagcggcag gttgccgtta tgaaaacgca tcccgttgtc
     7981 gcgcgggaaa tccacttcat gccgtccctg tgccagcagc agacgttcgc gctcttttac
     8041 gtcgcgaata aaaccgggaa tactgtcgat atccacggtg gcaggttcgt tacctgcaag
     8101 acccataata atggcgatat cggtgtggtg gcctttaccc gtcagcgaca gtgaaccata
     8161 aacgtccacg gcaacgcgag taacgctatc cagtaagcct ttttcgacca gatcatcgac
     8221 gaactgttta cccgccttca taggccctac ggtatgggaa gatgagggac caatccccac
     8281 cttaaacatg tcgaatagac taatcacgat aatactcctg acaaggtgac tggacttcca
     8341 gtaacgaact aataacagcg catagtgtaa gagggaacgc ggcgactggc ttaactattc
     8401 acatgaatta aactaatcaa aaaccgcgat ttaactaata ttgcgatgat tgtctcaagt
     8461 agtgttttgt agatgtaaag ataagtatag tcagggggtc acaccaattt gcagcgccag
     8521 ctcacgaatt atgcctgcgg tcattcccca tacaaaatac tgttcgtacc aggacagcca
     8581 tacccgatgt gaatcgccac ggcggtagat atctaaaggg tgataacgac ccagatgtaa
     8641 tgcctgggcg agcggcattt caaacaccgc cgagacttca tcttcactgg cgcgatacgg
     8701 cagatcgggc gggataatgc cgaccactgg ggttacttga tagccagtta cgctatcgac
     8761 gggcggcagc acgccgataa cttcaacggc ggaaggcggt atagcgacct cttcttcagc
     8821 ttcgcgcagc gcggcggcga taactgatgc gtccgtgtca tcgactgcac ctccagggaa
     8881 tgccacttgt ccagcgtgtt tacgcagatg aattgaacgc tgagtcagca acaaccccgg
     8941 ttgcggtcga cggacgatgg ggattaacac ggcagcctga cgatgattta ggggttcccg
     9001 gttaatttgc gggcgcaaaa gttgaaagcg agataaaaaa tcatcaagcg tcaggctacg
     9061 gtattccacg tcttacttct ccagttggcg taatatctta ttaactttat caaaagtttc
     9121 ctgatattcc gcttcttcct ggctatcggc gacaattcca ccgcccgcag agcagtatat
     9181 ttgtccgtta atggcagtca gcgtgcggat agtaatgctg gtatccatgt tgccgcaaaa
     9241 gctcaaatag ccaatgctgc cgcaccaggc attacgtcgc tgcggttcca gttcgtcgat
     9301 aatttccata gcccgtactt tcggagcccc ggttattgag ccaccaggaa aagctgcgcg
     9361 cagcagatcg ctggcgtgta actgttctgg tagtcgcgcc gttatagtgc tgaccagatg
     9421 atgcacggca gggaagggtt ccaccacgaa gagctctggt acttttaccg aaccggctac
     9481 ggcaacacga ccgatatcat tacgcattaa atcgacaatc atcagatttt cggcacgatc
     9541 tttcgctgag ttcgccagtt tttctgcttg tttgctatct tcctgaggat cgggcaggcg
     9601 tggtagcgtg cctttaatcg ggcgagtctg gatttcacta ttatcacaaa gaataaaccg
     9661 ctctggcgaa aggcttaaaa ttgcaccctg ttcaagacgt aaaaaagcgc taaatggcgc
     9721 gcggttggcc tgattaagct gaaggaatgc ctgccattca tcgccagaat aggtcgcatg
     9781 aaaacgctgg gcgagattca cctgatagca atcaccgctg tgcagatatt cctgtacctg
     9841 gcgaaatttt tcgccgtact gctcgtgggt catattggat tgccagtcgg aagtgagcgt
     9901 gaaatcttcc tgcggcgaga attgctggct ttccagccag gcccgacggg cattgacatc
     9961 attatgactc agcaaagaaa ctgtatgacg ctggtggtcg acaatgagcg cccaatcgta
    10021 gatacccact gccatatccg gcagaacgat atcttgttcc gcaatttctg gcagtgactc
    10081 aaaacggcgg cccagatcgt agccaaacaa ccccagtgcg ccgccctgaa atggcaaatc
    10141 ttcgttatgc gttgggcgaa tgtctgcgcg atccagcacc tgctggagca cctgtagcgg
    10201 gtcatcagtg gtcgttgtgc gtttttcgct ttcactaaca acggtttctt tacctaaagt
    10261 ggttaaagtg caaatcggat cggcgaccac aatatcaaag cggctatacg gatgatcggc
    10321 atagccggag tgtaaaagca tcgcccacgg caggtggctt aagcgggaga aataaaattc
    10381 agcggcgtcc tgacgccagg gtaaagtaat cacagtggga gataacgtct tcatcagtcc
    10441 tgactctact ggctatgtgc cgggtagcat gaaataatta gcgccaacaa tttagcagga
    10501 gttaacaatg tttgcaggtt taccttcact catccatgaa cagcagcaaa aagctgtcga
    10561 gcggatccag gaactgatgg cacaagggat gagtagcggc caggcaatcg cgctggtggc
    10621 agaagagctg cgtgccaacc actccggcga gctaatcgtg gcgcgttttg aggatgaaga
    10681 cgagtaacac actaatccgt atcgatttcg ttatagaagc cgatgatcat ttgattgtga
    10741 gaaaaaggac gctttttata tgtgattaaa agcgtcctga cccatttatg ctgatataaa
    10801 tttaactcag accagtgctt ttagttcttc ctgcatatat gttgaaaagt attcaggatt
    10861 tttaatcaca gagcgttcac ctgtctccat tttttttgcc catccaacca ttttttccgt
    10921 gaacccaggg ttccagcctt cctggtcagc tcgcgccgta atatcagcgg cactcgcggg
    10981 tactcccagt tcttttaaat atttaaagat tcccttcgcc gtgctttcat tcatcgaact
    11041 tggtgttggc gctgatacat ccatggcacc aatgaaatcc agggctttat ctattacagc
    11101 aggcatattc ttatccttaa aattaaccat gttatcgata aggctatata tgaccttaat
    11161 ttgcatctaa tatcaaaggg attataacga tatggcacga gttaacctgt tgacattcag
    11221 gtgttttaaa ggtagcagct catggtgtgt tatccagtaa gatggagaaa attaacggtc
    11281 agcccgcacg ctataaccag attgttcagc taagaaagaa aaccagagtt gctaatcaaa
    11341 gggcgaacac aacggcatgg ttatgtttat ttatatagtt agttttatag ataaaattga
    11401 aattataaat ataaataatg aattagcaga agcatcaaca gtaatatctt attctctggt
    11461 cgatttatgt aaaaccgacc agtattatcg ataaattatc gtctgactta ttttgcttcg
    11521 ataaagctta caccgcagcc acaatcttaa tttcaacttt atacttcggg ttcatcaaac
    11581 ccgcttgtac cgtgcagcgc accggcgcat gacccgcgac aacccaggca tcccacgctt
    11641 tattcatcgc cgcgaagtcg tttttatcgg ccaggaaaat ggtggcatcc agaatgctcg
    11701 atttattgct gccctgtttt tccagcacgg cgtcaatctg tgccagcgtg ttggcggttt
    11761 gctcaaaggc atcggcgtcg aggttttccg gtacaccagt gtagtagagc gtgttgttgt
    11821 ggattactac atcagaccag cgggcttcag catcgatacg aacgatagtc atcattttgt
    11881 cctcattata cttccatttt cgtgctgcaa agactgccac aatgtgaggt tgccgtcacc
    11941 acttcaactg gcgaaagcgc cctcatcctg acataatccc tgttcaaatc aacagggggt
    12001 agtgtgacgg acgattttgc accagacggt cagctggcga aagcgatacc aggctttaag
    12061 ccgcgagaac cacagcgaca gatggcggta gccgtcaccc aggcgataga aaaaggccag
    12121 ccgctggtgg tggaagcagg aaccggtacg ggcaaaacct acgcttacct ggctcctgcg
    12181 ctgcgggcga aaaagaaagt cattatctcg accggctcaa aagcgttgca ggatcagctc
    12241 tacagccgcg atttgccaac agtctcaaag gcattgaaat atacgggcaa cgtggcgctg
    12301 ctgaaagggc gctcaaacta cctctgcctc gaacgtctcg aacagcaggc gctggcgggg
    12361 ggcgatctgc cggtacaaat cttaagcgat gtgatcctgc tgcgctcctg gtctaatcaa
    12421 acagtcgatg gtgatatcag cacctgcgtc agcgtggcgg aagattcaca ggcgtggccg
    12481 ctggtcacca gcaccaacga caactgtctt ggcagcgact gcccgatgta taaagattgc
    12541 tttgtggtca aagcacgtaa aaaagcgatg gacgccgatg tggtggtggt aaaccatcat
    12601 ctctttctgg cggatatggt ggttaaagag agtggatttg gcgagttgat ccctgaagcg
    12661 gacgtcatga tcttcgacga agcccaccag ctaccggaca ttgccagcca gtattttggt
    12721 cagtcactct ccagtcgaca actgctcgac ctggcaaaag acatcaccat cgcctaccgc
    12781 accgaattaa aagacaccca gcagttacaa aagtgcgctg atcgtcttgc ccagagtgcg
    12841 caggattttc gtctgcaact cggtgagcca ggttatcgcg gtaacctgcg tgagctgtta
    12901 gctaatccgc aaattcagcg ggcattttta ctgctcgatg acaccttgga actttgttat
    12961 gatgtggcga aactgtcact ggggcgttcc gccttgctgg atgcggcatt tgagcgcgcc
    13021 acgttgtatc gcacacggct gaagcggcta aaagagatca atcagccggg ctacagctac
    13081 tggtacgaat gcacttcgcg ccattttact ctggctctca cgccgctcag cgtggcggat
    13141 aaattcaaag agttaatggc gcaaaagccc ggtagctgga tcttcacctc agcaacgctg
    13201 tcggtgaacg acgatctgca tcatttcacc tcgcggcttg ggatagaaca ggcggagtcg
    13261 ttgctattgc caagcccgtt tgattacagc cgccaggcgt tactctgtgt gccgcgcaat
    13321 ctgccgcaaa ccaaccagcc aggttctgct cgccagttag cggcaatgct gcgaccgatc
    13381 atcgaagcta acaacggtcg ttgttttatg ctttgtacct cgcacgccat gatgcgcgat
    13441 ctggccgagc agttccgcgc taccatgacg cttcccgttt tgttgcaggg ggaaaccagt
    13501 aaaggccaac tgttgcagca gtttgtcagt gccggtaatg cgcttcttgt ggcaaccagt
    13561 agtttctggg aaggggtgga cgtgcgtggc gatacattgt cattggtaat tatcgacaaa
    13621 ttgccgttta cctcgccgga tgatccactg ttaaaagcgc gcatggaaga ttgtcgtttg
    13681 cgcggtggcg acccgttcga tgaagtgcaa ctaccagatg ccgtcattac tctcaaacag
    13741 ggggtaggtc gactgattcg cgacgccgac gatcgtggcg tgctggtgat ttgtgactat
    13801 cggctggtga tgcgtcctta cggcgcgacg tttctcgcca gtctgccgcc cgcgccacgc
    13861 acccgtgaca ttgcccgtgc ggttcgtttc cttgcgatac catcctccag gtaatttgtt
    13921 acggagtatg gtatgatgcg cgccttttta ttgatctaag cacgaagacc catgcgaatt
    13981 ctggctatcg ataccgcgac agaggcctgc tctgtcgccc tgtggaacga cggtactgtc
    14041 aacgctcatt ttgagctttg ccctcgtgaa catactcaac gaatcttacc gatggtgcag
    14101 gatatcctga ccaccagcgg aacttccctg actgatatta acgctctggc ttacgggcgc
    14161 ggccccggta gctttaccgg cgtgcgcatt ggtattggca tcgcgcaagg gctggcgctt
    14221 ggcgcggaat taccgatgat tggcgtctcc acgctaatga cgatggcgca aggggcgtgg
    14281 cgcaaaaacg gcgcaacacg cgtgctgtca gccattgatg cgcgaatggg cgaagtttac
    14341 tgggccgaat atcagcgtga tgaaaacggt atctggcacg gtgaagaaac cgaagccgta
    14401 ctcaaacccg aactcgttca tgaacgaatg caacagcttt ccggcgaatg ggtgacggta
    14461 gggacgggct ggcaagcctg gccggatctc ggtaaagaga gcgggctggt tttgcgcgat
    14521 ggcgaagtgt tactccccgc tgcagaagat atgctgccga ttgcttgtca gatgtttgcc
    14581 gagggtaaaa cggtggcggt ggaacatgcc gagccggttt atttgcgtaa caacgtcgca
    14641 tggaagaaac ttccgggcaa agaatgaatc ttaagagtat gccctgagaa aaaaaggagt
    14701 cgcatcatgg cggttcaaaa gaatgttatc aaaggcatac tggcaggtac gtttgcgcta
    14761 atgctgagcg gttgtgtcac tgtgccggac gccattaaag gcagcagtac cacgccgcaa
    14821 caagatttag ttcgggtgat gagtgcgccg cagctgtacg ttggtcagga ggcacgcttt
    14881 ggtggcaaag tggttgcggt acaaaaccag caagggaaaa cccgcctgga aattgctacc
    14941 gtaccgctgg acagcggagc taggccgacg ctgggagaac cttctcgcgg tcgcatttat
    15001 gccgatgtga acggttttct ggacccggtg gatttccgtg gacaactggt tacggtagtc
    15061 gggccaatca ccggtgcggt tgacggcaaa atcggcaata cgccctataa atttatggtg
    15121 atgcaagtaa cgggttacaa acgttggcat ttaacccagc aggtgattat gccgcctcag
    15181 ccgattgatc catggtttta tggcggtcgt ggctggccct atggctacgg cggatggggc
    15241 tggtataatc ccggccccgc gagagtacaa acagttgtaa ccgaataatt gcttgttttt
    15301 aaagaatgca gaaacagcgg ctggtccgct gtttctgcat tcttacggta aagataaaaa
    15361 taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt atcaagctgg tatgatgagt
    15421 taatattatg ttaacggcgt gtatatcatt tggggttgcg atgacgacga acacgcattt
    15481 tagaggtgaa gaattgaaga aggtttggct taaccgttat cccgcggacg ttccgacgga
    15541 gatcaaccct gaccgttatc aatctctggt agatatgttt gagcagtcgg tcgcgcgcta
    15601 cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg accttccgca agctggaaga
    15661 acgcagtcgc gcgtttgccg cttatttgca acaagggttg gggctgaaga aaggcgatcg
    15721 cgttgcgttg atgatgccta acttattgca atatccggtg gcgctatttg gcattttgcg
    15781 tgccgggata atcgtcgtaa acgttaaccc gttgtatacc ccgcgtgagc ttgagcatca
    15841 gcttaacgat agcggcgcat cggcgattgt tatcgtgtct aactttgccc acactctgga
    15901 aaaagtggtt gataaaaccg ccgttcagca cgtaattctg acccgtatgg gcgatcagct
    15961 atctacggca aaaggcacgg tagtcaattt cgttgttaaa tacatcaagc gcttggtgcc
    16021 gaaataccat ctgccagatg ccatttcatt tcgtagcgca ctgcataacg gctaccggat
    16081 gcagtacgtc aaacccgaac tggtgccgga agatttagcc ttcctgcaat acaccggcgg
    16141 caccacaggt gtggcgaaag gcgcgatgct gactcaccgc aatatgctgg cgaacctgga
    16201 acaggtcaac gccacctatg ggccgttgct gcatccaggt aaagaactgg tagtaacggc
    16261 actgccgctg tatcacattt ttgccctgac cattaactgc ctgctgttta tcgaactggg
    16321 tgggcagaac ctgcttatca ctaacccgcg tgatattcca gggttggtaa aagagttagc
    16381 gaaatatccg tttaccgcta tcactggcgt taacaccttg ttcaatgcgt tgctgaacaa
    16441 taaagagttc cagcagctgg atttctccag tctgcatctt tccgcaggcg gtgggatgcc
    16501 tgttcagcaa gtggtggcag aacgctgggt gaaactgacc ggacagtatc tgctggaagg
    16561 ttatggcctg accgagtgcg cgccgctggt gagcgttaac ccgtatgata ttgattatca
    16621 tagcggtagc atcggtttgc cggtgccatc gacggaagcc aaactggtgg atgacgacga
    16681 caacgaagta ccaccgggtc aaccgggtga gctttgtgtc aaaggaccgc aggtgatgct
    16741 gggttactgg cagcgtccgg atgctacaga tgagatcatc aaaaatggct ggttacacac
    16801 cggcgacatc gcggtgatgg atgaagaagg attcctgcgc attgtcgatc gtaaaaaaga
    16861 catgattttg gtttccggtt ttaacgttta tcccaacgag attgaagatg tcgtcatgca
    16921 gcatcctggc gtacaggaag tcgcggctgt tggcgtacct tccggctcca gtggtgaagc
    16981 ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc gaagagtcac tggtgacttt
    17041 ttgccgccgt cagctcacgg gatacaaagt accgaagctg gtggagtttc gtgatgagtt
    17101 accgaaatct aacgtcggaa aaattttgcg acgagaatta cgtgacgaag cgcgcggcaa
    17161 agtggacaat aaagcctgag cgttaagtca gtcgtcagac gccggttaat ccggcgtttt
    17221 ttttgacgcc cactaaagag aaaacaattt gaattaccaa atgattacca cggacgatgc
    17281 gctggcttct ttgtgtgaag ccgtccgtgt ctttccggcg atagccctgg atactgaatt
    17341 tgttcgtacg cgcacttatt acccgcagct ggggttgatt caacttttcg atggcgagca
    17401 tctggcgcta attgatcctc tcgggatcac cgactggtca ccgctgaaag cgatcctgcg
    17461 cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa gatctggaag tgttcctcaa
    17521 tgtctttggc gaattaccac aacccttgat tgacacgcaa atccttgctg ccttctgcgg
    17581 acgcccgatg tcatggggtt tcgcttccat ggtggaagag tattccggcg ttacgctgga
    17641 caagagtgaa tcgcgcaccg actggctggc cagaccgctg accgaacgtc agtgtgaata
    17701 cgcagcggcg gatgtctggt atctgttacc gatcaccgcc aagcttatgg tagaaacgga
    17761 ggcctccggc tggctacctg cggcgctgga tgaatgccgc ctgatgcaaa tgcgtcgtca
    17821 ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc aatgcctggc agttacgcac
    17881 acgccaactg gcctgtctgc aactgttagc cgactggcga ctgcgcaagg cgcgagagcg
    17941 tgatctggcg gtgaactttg tcgtgcgtga agagcatttg tggtcggtcg cgcgttatat
    18001 gccgggaagt ttaggcgaac tggacagcct gggtttatcc ggtagcgaaa tccgctttca
    18061 cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca ttgccggaag atgccttacc
    18121 gcagccgatg cttaacctga tggacatgcc tggttatcgt aaagcgttta aagcgattaa
    18181 gtcgctgatt actgacgtga gcgaaacgca taagatcagc gccgaattgc tggcatcgcg
    18241 tcggcaaatc aaccaactgc tgaactggca ctggaaactg aaaccgcaga acaatttgcc
    18301 ggagctgatt tccggctggc gtggtgagct gatggcggaa gcattacaca atttattgca
    18361 ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg cgctcagcgc cacatccggc
    18421 tttggcaaac tacaaatcca acaccaggcg tttacccttc gcacgcgaac aacagatcaa
    18481 catactttgc tggctggcac gctcttcatc gctaaaatac tgatcccgat ggtcagcttc
    18541 accttccagt attgccgttt cgcaggttcc gcatacccct tcacgacata aacattccac
    18601 tttcgcggct ttattatttt caataacctg caaaatagtc atctcttccg gcaccacaaa
    18661 ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg ccggttttgt cttcgatagc
    18721 aaattgctca aagtgcagtg tatcggcggc gatgtccaga cgcgcagctt cacttcttac
    18781 cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt gtgccaggtt cgatatccgc
    18841 caataatcgc gctaattcca gtcgtgttcc ggttgatgaa tgatgcaaat ggactttctc
    18901 agcctgcgga tgctggacta gctcagcacg aaatgcgcaa ctttctggat ttcgcgagca
    18961 gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct gccatgtgcg acaggaaagg
    19021 ggtgatacca ataccgccca cgataaacag atgctttctg gctgagggaa ttagcgcaaa
    19081 caggttatta ggcgttgaga tcgttaaccg atcgcccact tttacctgct gatgcaaaaa
    19141 gcgggaaccg ccgcgcgagt tttcttccag tcgaacggca atctgataac tagaggtgtt
    19201 atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg ttatcaccat cgctcatctg
    19261 cactaggatg tgacttcctc cggtgaacgc aggcagcggt ttgccatcgg ttgccaccag
    19321 cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg ctcacctgta cttcaaacat
    19381 ttgatagtct gacattgctg tactccatca aaaatcagcc cagcggcgga aaataccgcc
    19441 gccgatgtca ttagtcctta aacacctgcg ccagcagatt atggaaatgg gcgataccat
    19501 gttcggaaat gccactaccg ctactgtcgg ccatgatgcg cccctgacca cgatagccac
    19561 gcgatttcag ccctttctgt acgctttcaa ccagacgtaa atcttccgga tggaacacat
    19621 cgcgatacca ctcaatcagc gatttttgct cgtcggttaa ctcttcattg gtgaagtaaa
    19681 tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa ttcataaatg accgtcatca
    19741 tccctttgat cggggtgacg ttcagcatcg tgcacggcca cagccagaaa ccgtggaatg
    19801 ccgcatccgt accctcttca aatttaaacg actgttcgga cggtttggcg aaaccgtatt
    19861 gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc aacctgtacg gagtcggaga
    19921 aacctagatg cgccggacca cagtgatagc actcgagata gttatcgaca atgttcttcc
    19981 agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt cagattgtgg acttccgggc
    20041 aggcttccag cactttcgcg cccagaccgg tgtattgatt cacttgaagt acgaaaaaaa
    20101 accgggagga cattggatta ttcgggttct gatgggatta gatttggtgg ggcttgcaag
    20161 cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga aagatagtaa aaaattgctt
    20221 ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat catttcaata acagcttcaa
    20281 aaaacgttca aaaccgataa caaccaggct gtaaccaaat gactcatatc acaaatcagc
    20341 tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg tgaagtatgt tttagctggt
    20401 tcatggttgt tatacggctt tttttacatc ttgtggttct tgtgaaggag ctacaagacc
    20461 ttcctgttca taaataccat actttgacaa aatcttttta cgagtatctg ttggtaatga
    20521 ttcaagcatt ttggctgttt gtaaggtttg ctgcgaaata ccttgcaaat cagcctggat
    20581 ggaatcatcg gaagaaaaaa tctcaggcat aagagcttca aatcctttct ttctgaacaa
    20641 ctcaatcgca gaatgcagtt ccttgtcctc taacaattcg acaattgcga tcagtttagt
    20701 tttcaatgtg ctgcggtcag gttgagaatt tttattctct tgattatttt tatgctcaac
    20761 cattggcatg ctctgacttt ggataaacca ctctattgga cgccctgttg cgtaggctat
    20821 ttgtgctaat cgatctaaag acgggtatgt cttgccagac aggtagtttc tgataacccc
    20881 atcagaaagt ccgcacttag ctgcaaacgc ccggcttgat tctccattca ttgcctcttc
    20941 aaggcgttcc ttgaaacgca ttatttccgg gggcataaaa atgcgctctg catttttgct
    21001 ttctttttca cctgtcatgc ccaatctctc tgttatataa agaattttac agaattcttt
    21061 gatttgcact ggttaaaata aaaatgcgca tagcttttga attgagcatt tttctgcgct
    21121 atcgtttttc ccgatagcgg tagccggtat cagttaccga aaacgttaag agagtaagga
    21181 tcgcagaatg tcagcaaaaa accaagactg gcacgcagag cagataaagg ctgctgttcg
    21241 tatgaagggg aagacactat ctcaattatc cagag
//
LOCUS       DAZPAB010000055        21116 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZPAB010000055 DAZPAB010000000
VERSION     DAZPAB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21116
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.055"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..1438
                     /gene="gadC"
                     /locus_tag="ACHUPG_001836"
     CDS             <1..1438
                     /gene="gadC"
                     /locus_tag="ACHUPG_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative glutamine/gamma-aminobutyrate
                     antiporter GadC"
                     /protein_id="HFR0861476.1"
                     /translation="KSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIFYYTFAAIM
                     FLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYFPTVLTFGA
                     VSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSKWCGIIGTI
                     IPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAYAGMEMNAV
                     HVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLLVAYDMLFE
                     WAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKTNRHGMGHH
                     LMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIYLRHSQPDT
                     KRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLVALFLLIPI
                     LIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            1472..1885
                     /locus_tag="ACHUPG_001837"
     CDS             1472..1885
                     /locus_tag="ACHUPG_001837"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HFR0861477.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKN"
     gene            1882..2280
                     /locus_tag="ACHUPG_001838"
     CDS             1882..2280
                     /locus_tag="ACHUPG_001838"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861478.1"
                     /translation="MNSTINVFGEPSKNLAMLIEEGNLNAPAILNDIKKHVNHLISLH
                     PVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAISIKNHLGQLNLLNSSNIGTLQ
                     INTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(2399..3093)
                     /locus_tag="ACHUPG_001839"
     CDS             complement(join(2399..2847,2847..3093))
                     /locus_tag="ACHUPG_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR0861479.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(3612..4985)
                     /gene="eutA"
                     /locus_tag="ACHUPG_001840"
     CDS             complement(3612..4985)
                     /gene="eutA"
                     /locus_tag="ACHUPG_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR0861480.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(4960..5439)
                     /gene="eutP"
                     /locus_tag="ACHUPG_001841"
     CDS             complement(4960..5439)
                     /gene="eutP"
                     /locus_tag="ACHUPG_001841"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR0861481.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(5452..5787)
                     /gene="eutS"
                     /locus_tag="ACHUPG_001842"
     CDS             complement(5452..5787)
                     /gene="eutS"
                     /locus_tag="ACHUPG_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR0861482.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(6080..8359)
                     /gene="maeB"
                     /locus_tag="ACHUPG_001843"
     CDS             complement(6080..8359)
                     /gene="maeB"
                     /locus_tag="ACHUPG_001843"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR0861483.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDSLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            8648..9598
                     /gene="tal"
                     /locus_tag="ACHUPG_001844"
     CDS             8648..9598
                     /gene="tal"
                     /locus_tag="ACHUPG_001844"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR0861484.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            9618..11621
                     /gene="tkt"
                     /locus_tag="ACHUPG_001845"
     CDS             9618..11621
                     /gene="tkt"
                     /locus_tag="ACHUPG_001845"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR0861485.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(11724..12767)
                     /locus_tag="ACHUPG_001846"
     CDS             complement(11724..12767)
                     /locus_tag="ACHUPG_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR0861486.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(12893..13468)
                     /gene="nudK"
                     /locus_tag="ACHUPG_001847"
     CDS             complement(12893..13468)
                     /gene="nudK"
                     /locus_tag="ACHUPG_001847"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFR0861487.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(13536..15515)
                     /gene="aegA"
                     /locus_tag="ACHUPG_001848"
     CDS             complement(13536..15515)
                     /gene="aegA"
                     /locus_tag="ACHUPG_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR0861488.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            15721..17438
                     /gene="narQ"
                     /locus_tag="ACHUPG_001849"
                     /pseudo
     CDS             15721..17438
                     /gene="narQ"
                     /locus_tag="ACHUPG_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            17640..20753
                     /gene="acrD"
                     /locus_tag="ACHUPG_001850"
     CDS             17640..20753
                     /gene="acrD"
                     /locus_tag="ACHUPG_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR0861489.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(20852..20911)
                     /gene="ypfM"
                     /locus_tag="ACHUPG_001851"
     CDS             complement(20852..20911)
                     /gene="ypfM"
                     /locus_tag="ACHUPG_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR0861490.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         5125 a         5400 c         5274 g         5317 t
ORIGIN      
        1 caaaagtaag aaaatgacag tggggacact cgctatcatg aatataactg ctgtagtaag
       61 tctacgattt ataccttctg aagcagaata cggcttaggt gccatttttt actatacctt
      121 tgctgcaatt atgtttttag tacctgttgc ctttgttgcg gctgagctag ctacaacata
      181 tccacaaaaa gggggggcat ttcgatggat aggagaagca tttggggctc gctggggttt
      241 tgtaggaatg cttatgacct ggctacaagt catcccctac tttccaacag tactgacatt
      301 tggtgcagtc tcggttgcat ttattgatgt tgatatgggg gtggcagaat caattgccag
      361 taataaatgg tatattttat ttttcgtact atttgtttac tggggagcta tttgtgttgc
      421 attacgtggc gttggtattt tctcgcaggt atctaagtgg tgcggtatta tagggacaat
      481 tattccagct attatccttg ttattcttgg tttctcctat ctgtggttca gcggtaaacc
      541 agcggtgatc ccattaggtt ggggtgatct tttccctaat atagctaact tccagaatat
      601 tgttcttgcc gccagcatat ttcttgctta tgcagggatg gaaatgaatg cggtccatgt
      661 taacgattta gataatccaa ctaaaaaata cccaattgca attaccatcg cttctatagg
      721 tactattgca atattcctgc tcagtacttt aggggtcgcc tttattattc ctacagataa
      781 aattagtcta actcaaagtt tgttggttgc ctacgatatg ctgttcgaat gggctggagt
      841 gccatggctt ggctctgtga tggcatttat gctagcaatt ggcgtactgg gtggggtggt
      901 tacttggatt gcagggccta atacaggtgt attagcgatc gcgaaagctg gttatttacc
      961 taagtttttc caaaaaacca atcgtcatgg aatggggcac catctaatgt ttgtacaagg
     1021 tataattgtc tcagttcttt ctgttacttt tgtgataatg ccttcagttc aagcagcttt
     1081 tcagatttta tcacagttaa cagttatgct ctatttagtt atgtatatgc tcatgttcgc
     1141 tagtgctatc tatctacgac attctcaacc tgatacaaaa cgcccatatc gagcccctgc
     1201 tttatttttc tgggcaacac ttggattctt agggtctttt ctggcttttg tattaagttt
     1261 tattccgccc gagcaaatcc caactggtag tccaaccagc tatgttcttt atttgttagt
     1321 attagttgcc ctatttttat taattccaat attaatttac gccttccgaa aatctgactg
     1381 gaaagatcca aactctgatt tcgaaccatt ttcttgggaa aaaagaacta gtaattaaat
     1441 tttccataag ctacaaaaga tgaggttaaa tatgtttagt gctgaatctc caattggtgt
     1501 aatagactca ggtgtcggtg gattgacatc ggtaaaggaa ataattaatt tgcttcctgg
     1561 tgaagatatt atctattgtg gagataatat gaatgcacct tatggcaacc gttctgccga
     1621 tgatatcatc agtttgacta aaaaaatgct gactttttta cagtcaagga atgtcaaact
     1681 agttgctgtc gcatgtaata ctatatcctc aactttggaa tccgaagaat acagcggata
     1741 tgctaagagc tttccgtttc ctatcttatc catcattgaa cctgcagtag aagatgtgat
     1801 tcgccagcaa tataaaaatg ttggtattat cgctaccgag tttactatta aaacggggtg
     1861 tcacaaagag ctaataaaaa attgaattca acaataaatg tatttggaga accaagcaaa
     1921 aatcttgcaa tgttaattga ggaaggtaat cttaatgctc ctgcaatctt gaatgacatc
     1981 aaaaaacatg ttaaccattt aatttccctc catccagtaa atgaaattat tctcggctgt
     2041 actcactatc caattgttca gaattttttt gaagcagttg caccggatat aaaatttatt
     2101 aatcctgcac atgatcaggc tatctctatt aaaaatcact tgggacagct aaatttatta
     2161 aactcctcaa atataggaac tttgcaaata aatactagtg gaagtatgga gatctacaaa
     2221 acggtactag gggaattgag cattacgaaa cctcatacat tcagtatcag gcagttttaa
     2281 atttatccgg tacaccgtaa aatcccgtcc ttctgaactg caccccaaaa gttggacacc
     2341 aactgagtaa ggtgcatttt atggacaaac ccagttacat actgggtagt gcatccaatt
     2401 agtagaacat gtgtttttcg ataaacgctc cgatcacttt ttcgtggatc tcaactgagc
     2461 gtgagaagca gattgtttta cgacccaacc gcttaatgcg ggtgcgtagc gtcagattat
     2521 tacgctcaat gcgttgggtg aatattttgc cggtcagatg cttattcttc ggcacctccc
     2581 ggccatagct gccccagtca tcgctggtca gcatgccgat gttgaagggt gtaagcagtg
     2641 ccagtagctc ccggcacgtt tgatcggttc ggggaccaaa agtgtaggcc agtacaccgc
     2701 ctgttttggt gttgtacgcg tacccgagcc aatgttgccg ggctttactg ccaacgtagc
     2761 tccattgctc atcaagctcg cagataaacg ccacatcagc atgggcaaca ggcgaagaag
     2821 ttattcgctt tggcgcgagt tttttaaagt ccggatgacg gtgttactac caattttcag
     2881 tgtcctggcg gtatcgcgaa ccccggcacc attaaaggcc atttcagtta tcagctcttt
     2941 catacccggc ttacgtgctt gataagtata agtgaactga aacacacggt ggcagtcacg
     3001 gcagcgaagt ctgtcacggc ctttagggtt ctgaccatgg cggtaaacct gagctgactg
     3061 acaacgggga caatgaatat taacgctggt catgagataa cctcaaaagc ccgtattata
     3121 catcagattc aactaattag aggcatcacc catccagcat tataaaacat actaacaata
     3181 actaaattat aataatcaca acttacaatc aaataataaa tgcataagat taacaatgat
     3241 tttttcgggt tatatttatc taaaaaagca gaacattatg taacttatta ttttctaagc
     3301 gaaaatttat tgtgcggtca gactaacaaa cttgcgtacc acccgaagaa catgcgaaga
     3361 tttaagtatt ctttcttttt tggtttttat ttttcatttt ctcttttctt ctctttccgg
     3421 ttctgttctg gttttggttt tttccttttt atttttattc tttcttcagg gttatgctaa
     3481 aacaccaaaa catttttcac cacaaaagca tccaatcatc ttgccagacc aatcacctta
     3541 accggataca ctgccggata taatcccact aacacccaga caccaagcac catacctgat
     3601 aactaccgta ttcaggaagg aaatgcgagt gatttcaccg tcaccggcac aaccgatccg
     3661 ccaaaaagag gcgtaccaat gtcgatatag tcccccgcgc ggacaatcac ttcgtcaatg
     3721 actgccaacg ggagttgttg tagctgtggg cgcaacaaca tgcccagagc tttaccaaag
     3781 tcctgcccgg ccaccaccag caggggatgc ggattcggaa aacgcgcgac gaaatcgacc
     3841 agcgcgttga tgaccgtcag aaccgcggcg taacgcacag gcagcgaggc gggaagcgcc
     3901 agcacgtaca cgtcagtttt gggatcaaga tccagctgaa gcagcgcctg ttgccaggca
     3961 ctcaccagat ccgtttcatc aatcgggatc gccaccggca aattgcgcag cggcagttgt
     4021 acgccctcca gccagattgt gctgccagag agcgaaaggg tatgtgcacc cgcgccaatc
     4081 accgtggagc gtacggtttg cgccggaaac tgcacattca tctcacgcag gcgcggatgg
     4141 tcatgcagcg ccgttgccag aagtgggcca atatcggcaa aacagaacgg gttggcgggc
     4201 tggtggcgat aacattcgcc cacgcctcca gaaagcgtaa tgatttcggg cgtaacacct
     4261 gcgggcagca aaccggtttg catcaatgcc tgcgcgagcg gcgaaagcgt tccgtcaatc
     4321 acttcgacaa tcagcgccgc catccgccgg gtaacctgca ccagctgcgc gccggtcagc
     4381 gaacgggcgt cagtgcctgc accgaagcac tcatccacaa tcatctgccc cggtttatga
     4441 gcgtaaacca cgcgcccctg gctgtcggtt tccagcaggc gaccaccgac gttgagacag
     4501 gcagtgccgc tgatttttcc ggcatcgaac agggcgtagt tcgcggtgcc accgccgata
     4561 tcgatattca gtacccgaca cagccgttgt tcagaaaggg tttgcgcccc ggctccgtga
     4621 ccggcgatca cggattcgag gtgcggcccg gcgctggcga cgacaaaatc gcccagcgac
     4681 tgtttttcct tcatcacttt ttccatcagc tgtgcgacca ggctttcggt aaactggcct
     4741 tccggcagct gggcaatgat ggtttcgcgg atgcgctggc tttcggtttt gtcggctggc
     4801 acgctggcgg gcacggacgc cgttaccggg atcgattcat cacattcggt gatggtaaaa
     4861 cccagtagat ccgccacttc acgggcttcc ggggtaataa tgctggcgcg cagaaccacg
     4921 gacattgcct gttcgccgcg cgcgtgtgct tcacgaatat cattcgctgt gatgagtttt
     4981 ttcacctgct tcctcctgtt tggtgagaga tgccagataa tccaccagtt gctgcacgct
     5041 ttgcgggtcg tggctgttta gctcgaaaat cggctcttcg aaacccgtct caagcaataa
     5101 ctttcgcgtt gcggcgacgt cagcatctgg catgtccgtt ttactgatga cggcgatttg
     5161 ccgtttgctg acgccaatat ccagcaaccc ggcaggcaac cgactttccg ggtcatttgc
     5221 gccatggaca tagatcagca tgtctacatc ctgtagcgtg gtaattaagg cgtgatacca
     5281 gcggggatgg ctaaaatatt cacccggcgt atcaatatcg cccttatcat taaattccac
     5341 ggccagtgtt tttctggcga gggtataatt cccctgcaac gcattaaata acgttgtttt
     5401 tcctgccccg acgctaccca caaacgcaat tcgtttcatg aatgcctcgt attaactttt
     5461 ggtcatttca caaagcgtat aatttaataa gcgacctaaa ccgctgacgg tttgtgataa
     5521 cgcttcttct actgcgccca ccgagccata aatcaccaat gcaccgctga agcggtcgag
     5581 aaaaccgata tgcacatcag cggctttcag cgctaaatca ccggcgatca tcgccgtttc
     5641 gccgggagtt agcgtcataa tgccaattgc gcccgcatcc ggaacgccga ttttcttcgc
     5701 cagttcctca ccgggatgcg cgatgagatg cgccagtgtg acctgtttgc cgggcacaaa
     5761 ttcctgaatg atgcgttctt tatccatgag tcgccgcctg tgaaaaactt tcgtgtatcg
     5821 tgacaatttt tcgtcagagt aaataacaaa attcggcaag cgcgtttaaa aggtgaggta
     5881 gatcaataag aatggagaga tatttactgt tatgaataaa ctttcatatg cgacgcatga
     5941 atataaataa aataatatcg agatatttta tatactgatt tttttattat tcgcaaactc
     6001 agtaataagc tttgctaata tatttcagga aaaccgctta ccctgaatat tcagggtaag
     6061 cgtgagagtt aagaatgaat tacagcggtt gggtttgcgc ttcaaccacg gccagcgcca
     6121 ccatattgac gatacgacgc accgatgcga tcggcgttaa cacgtgaacc ggtttcgcca
     6181 cgcccatcag caccggaccg acagtcacac cttccgagct ggaaacgcgc agtaagttgt
     6241 aactaatgcg ggcagcttcc atgttcggca tcaccagaat attggcagaa cctttcaaag
     6301 ggctgtccgg catacggtcg ttgcgaatcg cttccaccag cgctgcatcg ccgtgcattt
     6361 caccatcaat catcagatct ggcgcacgat ccctgaccag ttccagcgcc tgacgcattt
     6421 tgctcgacga cgggcagtca gaagaaccaa agttggagtg cgacaacaga gcaacgcgcg
     6481 gctcaatacc aaaacgacgg acagtttctg ccgccatcaa ggtgatctcc gccagctctt
     6541 ctgcgtctgg ttcgtcattg acgtaggtat cggcaataaa ggtgttacca ctcggcagca
     6601 gcagcgcatt cattgcacct gcggtgtgaa cgccatcgcg ataaccaaag acatttttca
     6661 ccacactaaa atgttcatga taatcgccca ccgtaccgca gatcattgca tcggcttccc
     6721 cacgctgaac catgatcgcg ccgatcactg tcgggttact gatcagcgca cgctgcgcct
     6781 gttcctgagt gacaccgcga cgcttcatga tctggaagta ttcggtccag tactctttaa
     6841 agcgcggatc ggattcgtta ttgacgattt caaaatcaac gcccgctttg atctgcaagc
     6901 ccagtttctg aatgcgcatt tcgatcacgt tcggacgacc gataaggatc ggtttcgcca
     6961 gtcccagcgt taccagttcc tgagtcgcat gcagaacgcg cgcctcttcc ccttccggca
     7021 gaactacgcg cttcggcgct ttgcgagcct gggagaaaat cggcttcata aacaggttgg
     7081 ttttgtaaac gaactcagtc agcttgtcga tgtagacgtc gaaatcagca atcggacgcg
     7141 tcgccacgcc cgactccatc gccgcttttg cgaccgcagg agcgatctta acgatcaagc
     7201 gcggatcaaa cggttttgga atgatgtatt ccggaccaaa gctcagatcc tgaacgccat
     7261 acgctgaagc caccacttca ctctgttctg catgggccag ttctgcaatc gcgcgtaccg
     7321 ccgccagttt catctcttcg ttgatggcgg ttgcgccaac gtccagcgcg ccacggaaga
     7381 tgaacgggaa gcaaagaacg ttgttcacct ggttcgggta gtcggaacgg ccggtacaga
     7441 tgatggcatc cggacgcact tctttcgcca gcggcggcag aatttccggt tccgggttcg
     7501 ccagcgccag gatcattggt gcccgagcca ttttcttcac catttcctgg gtcagcactt
     7561 tcgggccgga acagcccagg aaaatatccg caccttcaat cacatcatcg agggtacgtt
     7621 tgccgtcatc caccaccgca tatgcggctt tggtttccgc catatttggc tcacggccct
     7681 gatagataac gccttttgaa tcgcaaacca cgatgttatg tttttgcagc cccagcgcta
     7741 ccagcaggtt catacaggcg attgctgcgg cacccgcgcc ggaaaccacc atccgcacgt
     7801 cggagatgtt tttctccacc acgcgcaagc cgttgaggat ggcggcagtg ctgatgattg
     7861 ccgtgccgtg ctgatcgtcg tggaataccg gaatattcat ccgctcgcgc agtttctgtt
     7921 caatatagaa acattctggc gctttaatgt cttcgaggtt gatgccgccg aaggtcggtt
     7981 cgagcgcggc gacgacttca ataaatttgt ccgggtcgag ttcatcaact tcaatgtcaa
     8041 atacatcaat cccagcgaat ttcttaaata gaacgccctt gccttccatc accggtttgc
     8101 ctgccagcgc gccaatgttg cctaacccca gcaccgccgt accgttagag atcaccgcaa
     8161 ccaggttacc gcgcgcggta tatttgtagg cttttagcga gtctttttcg atttcaagac
     8221 aaggtgcggc aacgcctggt gagtaggcca gcgccagatc gcgctgcgtt gccagaggct
     8281 tggttggaga aacctggatt ttccctggaa ctggaaattc atggaaatca agtgcacttt
     8341 gttttaactg gtcatccatt tggttgttcc tttcacgtaa cgttcacaaa taaagtgtgt
     8401 gggcaacagc ccctgcccac aacgtggcgc acattattac cctgccggag tctacagact
     8461 ttgagcaagt ccaaactctc accattaata taatgttttg gtaataatcc tataacactg
     8521 atgttacctg cttaatccag caataccatg cctgtctgct atgctttttt gatgcgttta
     8581 gcgaaatttc tcagaagtgt gaattaacgc actcatctaa cactttactt ttcaaggagt
     8641 atttcctatg aacgagttag acggcatcaa acagttcacc actgtcgtgg cagacagctg
     8701 cgatattgag tccattcgcc attatcatcc ccaggatgcc accactaatc cttcgctgtt
     8761 actcaaggct gccggattat cacaatatga gcatttaata gacgatgcta tcgcctgggg
     8821 taaaaaaaat ggcaagaccc aggaacaaca ggtggtcgca gcgtgtgaca aactggcggt
     8881 caatttcggt gctgaaattc tcaaaatcgt acccggttgc gtgtcaacag aagttgatgc
     8941 acgcctctct tttgataaag aaaagagtat tgagaaggcg cgccatctgg tggacttgta
     9001 tcagcaacaa ggcgttgaga aatcacgcat tttgatcaaa ctggcttcga cctgggaagg
     9061 aattcgcgcg gccgaagagc tggaaaaaga aggtattaac tgcaacctga cgctgctgtt
     9121 ttcttttacc caggcgcggg cctgtgcgga agcaggcgtt tttctgattt cgccgtttgt
     9181 agggcgtatt tatgactggt atcaggcacg caagccgatg gacccgtatg tggtggaaga
     9241 agatccgggc gttaaatcgg tgcgcaatat ctacgactac tataagcaac accactatga
     9301 aaccattgtg atgggcgcga gcttccgtcg caccgaacaa atcctcgcct taaccggctg
     9361 cgatcgactg actatcgcac cgaatttgct gaaggagctg caggaaaaag tttctccagt
     9421 ggtacgtaaa ttaatcccgc cttctcagac gttcccacgc ccagcaccca tgagcgaagc
     9481 ggagttccgt tgggagcaca atcaggatgc gatggcggta gaaaaactgt ctgaaggcat
     9541 tcgtctgttc gctgttgatc aacgcaaact ggaagatctt cttgccgcca aactataaac
     9601 cagccacgga gtgttatatg tcccgaaaag accttgccaa tgcgattcgc gcactcagta
     9661 tggatgctgt acaaaaagcc aattctggtc atcccggcgc gccgatgggc atggctgata
     9721 ttgccgaagt gctgtggaac gattttctta aacataatcc taccgaccca acctggtatg
     9781 atcgcgaccg ctttattctt tccaacggtc acgcgtcgat gctgctctac agtttgctac
     9841 atctgaccgg ttacgacctg ccgctggaag aactgaagaa cttccgtcag ttgcattcga
     9901 aaaccccagg ccacccggag attggctata cgccaggcgt tgaaaccacc accggcccgc
     9961 ttggacaagg tttggcgaac gccgtcgggc tggcgatagc agagcgtaca ctggcggcgc
    10021 agtttaacca gccagaccat gagatcgtcg atcacttcac ctatgtgttt atgggcgacg
    10081 gctgcctgat ggaaggtatt tcccacgaag tctgttcgct ggcaggcacg ctgggactag
    10141 gcaagctgat tggtttttac gatcacaacg gtatttccat cgacggtgaa acagaaggct
    10201 ggtttaccga cgatacggca aaacgttttg aagcctatca ctggcatgtg atccatgaaa
    10261 tcgacggtca cgatccgcag gcggtgaagg aagcgatcct tgaagcgcaa agcgtgaaag
    10321 ataagccgtc gctgattatc tgccgtacgg tgattggctt tggttcgccg aataaagcag
    10381 gtaaggaaga ggcgcacggc gcaccactgg gggaagaaga agtggcgctg gcacggcaaa
    10441 aactgggctg gcaccatccg ccatttgaga tccctaaaga gatttatcac gcctgggatg
    10501 cccgtgaaaa aggcgaaaaa gcgcagcaga gctggaatga gaagtttgcc gcctataaaa
    10561 aggctcatcc gcaactggca gaagagtttc cccgacggat gagcggtggt ttaccgaagg
    10621 actgggagaa aacgactcag aaatatatca atgagttaca ggcaaatccg gcgaaaatcg
    10681 ctacccgtaa ggcttcgcaa aatacgctta acgcttacgg gccgatgctg cctgagttgc
    10741 tcggcggttc ggcggatctg gctcccagca acctgaccat ctggaaaggt tctgtttctc
    10801 tgaaggaaga tccggcgggc aactacattc actacggggt gcgtgaattt ggcatgaccg
    10861 ctatcgccaa cggcatcgcg caccacggcg gctttgtgcc gtataccgcg acgttcctga
    10921 tgtttgttga atacgcccgt aacgccgcgc ggatggcggc actgatgaaa gcgcggcaga
    10981 ttatggttta tacccacgac tcaattggcc tgggcgaaga tggtccgacg caccaggctg
    11041 ttgagcaact ggccagcctg cgcttaacgc caaatttcag cacctggcga ccgtgcgatc
    11101 agatggaagc ggcggtgggc tggaagctgg cggttgagcg ccacaacgga ccgacggcac
    11161 tgatcctctc aaggcagaat ctggcccagg tggaacgtac gccggatcag gttaaagaga
    11221 ttgctcgtgg cggttatgtg ctgaaagaca gcggcggtaa gccagatatt attctgattg
    11281 ccaccggttc agagatggaa attaccctgc aagcggcaga gaaattagca ggagaaggtc
    11341 gcaatgtacg cgtagtttcc ctgccctcga ccgatatttt cgacgcccag gatgaggaat
    11401 atcgggagtc ggtgttgcct tctaacgttg cggctcgcgt ggcggtggaa gcaggtattg
    11461 ccgattactg gtacaagtat gttggtctga aaggggcaat tgtcgggatg acgggttacg
    11521 gggaatctgc tccggcggat aagctgttcc cgttctttgg ctttaccgcc gagaatattg
    11581 tggcaaaagc gcataaggtg ctgggagtga aaggtgcctg atggtgatcg ccggatgctg
    11641 tatgttcccc tcaccctaac cctctcccca gaagggcgag gggacggtcc gtgcgctttt
    11701 tagggccgca tctgcgattt tgtttaacgg gtgatccaca acgtgggcct ggcgtctggc
    11761 ccatgccagt tatcgcaggt aggttctgcg gcgtaacgca ccaggcgaaa acgctgaccg
    11821 tcaaagcgcc agcgcgcctg aatgccacaa tcgcttaatc cgcgcccttt cgctaaggtc
    11881 accagttcac gcgatttctc atcaaatgtt gcgttcatca gttccagttc attcgtctcc
    11941 tggccgttgt tgaacggcaa acgcaaccga accgggcgcg aggctagcgg ctttttacgc
    12001 gacacaatcc atgccaaatc aatggtgtta taggcccctg cctcacagct aatcatcatc
    12061 agtgctttat catcggtcag cgcagtgaca ttcacctcac gacgtaacgg atcaagcgag
    12121 cagcgcagac cattcatccg ccagtttcca taatccagca aatcgttacg ttcttcgagt
    12181 gagagtggcg tcggcgttgg gttaaccacc gcgacctctt tcagcgcagg cgcgggcggt
    12241 acgctgagag gcggttcgtc ccctttcttg atccacgcgg tttcactgcc aacgcgcttt
    12301 tgctgagcat caataaacaa caacgccgct tttaagccgc tcagagaaat ggtctgattg
    12361 ccgtcgcgta aggtgatcgc cttcccttcc tgaattatct gcaaaaacgc ggtgatggtt
    12421 gccgtatcat cggttactaa taaccatggt gaaatccgcc acttgtcacc acttaatgcc
    12481 aacggctcac catctaatag cagccgtggc gctatctccc cttcagacgc atccggcgac
    12541 ttcaacccac cgcgctcaat acgtaaaacg gcatcggtat gcgccccagc gctgcggctc
    12601 agggtcatca ccagtccatt atgatcgccc gtattgcgcg ccacacaaaa attttgatta
    12661 ttacaggtga cctgccagtc ggaaaacgcc cgttgcgctg gtgccgccca caccaaagac
    12721 gtcggcaaca aagcgaaaaa aaagagaagg aaaatgcgat agcgcatgga cggtacgacc
    12781 ccagaagttc aaacaaacag cacaagtcgt atcttcgttg cgcaggtagc gcggctcaat
    12841 cggatttgtc ggattgagcc aataataaac aacgaaatat tattgctatt tttcagtcca
    12901 ttaaatgtga catttgcaaa tagttaagca ataacaccgt cttaccgtca cgtatctcgc
    12961 cggttttgat catctccagc gcctggctga acggcagctc gagcacttca atatcttcat
    13021 cttcaacacc gccaccggcg ttagcgcgct gattgtcact gtattcggcg ataaaaaagt
    13081 ggattagctc agtcacaccg ccaggcgaca tatacagttc aaataatttg cgcacttcgc
    13141 ccacttcata gcccgtctct tcaatcgctt ctttacgaat gcacacttcc ggttcgtcgt
    13201 tatccagcag cccggcgcag gtttcaatca gctgcccgct ttcattgcca ttaacccagg
    13261 tagcgacacg gaactgacga atcagaacca cgctcttttt cttcgcgttg tacaggagga
    13321 tcgtcgcacc attaccgcga tcgtaaactt cacgtttatg gcggataact tcgccatctt
    13381 tgcgcgtgag atcgtaagta atgttgtgca gggtgaaata gttatcggag agaattttgt
    13441 ctttaatgag ggtgatttgt tgcgtcatac cgactccaca gcgcgaaatg aacaattata
    13501 ttacgctgtg aagtcgggtt tgtctgcgca ggctatcagt gagatttgac tgattttacc
    13561 cccagccagt caataatccc ctgtgccgca tgacgtcctt ctgccattgc ggtaaccacc
    13621 agatccgcac cacgcacggc gtcgccaccg gcgaagattt tcggattggt ggtctggtaa
    13681 cggtactggc tttccacatc cgcgatgatg cggccccatt tgtctaccgt tacaccgtgc
    13741 gactccagcc acggcatccc gtgtggattg aagccaaacg ccataatcac cgcatcggct
    13801 ggcatgacaa attcactgcc ctcaatcggt acaggtcgcc gacgcccctg ggcatccggc
    13861 tcgccaagac gcgtgcgcag gaaacgaatt ccgcaaacgt gaccttgctc attcagctca
    13921 agcgccaccg gctggacgtt aaattcgaag ttggccccct cttcgcgggc gttcttcacc
    13981 tctttcttcg agcctggcat gttagcttca tcacgacgat aagcgcaggt gacgttactc
    14041 gcgccgtggc gcagcgcagt acgcacacag tccatcgcgg tgtcgccgcc gcccagtacc
    14101 acgacgttaa gtccggcggt attgataaac ggctcttccg gtagctcttc gagccccatc
    14161 acctgtttag tgttggcaat gaggaacggc agcgcgtcat aaacgcccgg cgcatcttca
    14221 ttgggtaaac ccgctttcat ggaacggtaa gtgcctacgc caacgaagac cgcgtcgtat
    14281 tgttccaaaa gcaaatccaa agagacatct ttacccactt cacaattgag ttcgaagtga
    14341 atccccatcg cgctgaagat ttcccgacgg cgtgccagca gggatttatc cagtttgaaa
    14401 gaaggaatgc cgaaagtgag caagccaccg atttctggat ggcgatcgta caccgtcacc
    14461 gccacgccat tgcgggtcag aacatccgca caggccagcc ctgccggacc tgccccgata
    14521 atcgccaccc gcttgtccac tttggtgaca tgacttaagt caggacgcca acctttcgcc
    14581 aacgcctgat ctgaaatata gcgttcaatg ttgccgatag ttaccgcgcc gtgctcatcg
    14641 cgaatagtac aggcaccttc acacaaacgg tcttgcggac aaacgcgtcc ggtaatttcc
    14701 ggcagggtgt tggtctggtg agaaagctcg acggcggcgt cgatgtttcc ggctttcacc
    14761 agttcaatcc actgcggaat atggttatgc agcgggcagg tccattcaca aacgctgtgc
    14821 tcgccgaact taaggcagcg cgaggcttcc cgttgtgcct ggtcggcgcg aaatggcaga
    14881 taaatttcat caaaaccggt tttgcgcgct tcaatcgcca gtttatccgg ctcgccacgc
    14941 gcgggcgttg cctgcatttg ttcgacttta ctcattaccg gcatttcttg tgcagcggta
    15001 ctggcatgcc acggttgatg ctcctgacgt gcggtgcgca agcggcggga tttcgccatg
    15061 ccggagagtg cgacgtcagt gaccagttgc agcgcgtccg ccgggcaatt ctcgacacag
    15121 gcaggaccgt tttcgcgccc tgcacaaagg tcgcatttat gcgccgtggc ttttactttt
    15181 cctgccgcga cgggtgtcag gacgatttgc atcgtaccaa acggacaggc caccacgcag
    15241 gatttacagc caatacactt ttgctgattg acctgaatgc tgtcatcaac gtggctgatt
    15301 gcgccattag ggcagctacg ggcgcagggc gcatcttcac aatggtgaca ggtcactgca
    15361 ctacgttgct gctgatgttt gataaccgta attcggggat gaaaatggtg ttggctcagg
    15421 acatgttgct catcattgtg agccatgaca caggcgattt cacaagcatg acaacccaga
    15481 cattgctgac tgttggccat aataaaacga ttcataacga ccttcttttt tggttgtaaa
    15541 aaccttattc tttatatgag tgttgttatt acccgactta caggggaacc ggcaatgttc
    15601 atgtgcccag aataagtaac tatttcgcta taaactgtgg cagatcaaat aatcccatca
    15661 ctgactaaat tgcgtttcag cgaactggaa cattaatgat tttttgtgga gaagacgcgt
    15721 gtgattgtta aacgacccgt ctcggccagt ctggcccggg ccttttttta cattgtgctg
    15781 ctgtcgattc tttccacggg tatcgctctg ctaactctgg cgagcagttt gcgcgacgct
    15841 gaggctatca atattgccgg atcgctgcgt atgcagagtt accgcctggg ctacgacttg
    15901 caaagtggca gtccacaact caatgcacat cgccagttgt ttcagcaggc actgcattca
    15961 ccggtattaa ccaacctcaa cgtctggtat gtgccagaag cagtaaaaac tcgctatgcg
    16021 catctgaatg ccaactggct ggagatgaat aatcggctca gcaagggcga tttgccgtgg
    16081 tatcaggcca atattaataa ttatgttaat cagatagacc tgttcgtgct ggctttacag
    16141 cactacgctg aacgcaaaat gctgctggtg gtggcgattt ccctggctgg cggtatcggt
    16201 attttcacgc tggtcttttt tactttgcgc cgcatacgcc atcaggtggt tgccccgctg
    16261 aatcagctgg ttaccgccag tcagcgtatt gaacacgggc agttcgactc gccgccgctg
    16321 gataccagtc tgccgaatga gcttggtctg cttgcaaaaa cctttaacca aatgtcgagc
    16381 gagctgcata aattgtacct ttcgctggag gcgtcagtag aagaaaagac ccgcgatctc
    16441 cacgagacca agcgtcgtct ggaggtgttg tatcagtgca gtgttcccag gcgctgaaca
    16501 ccagccagat tgatgtgcat tgtttccgcc atattttgca gattgttcgc gacaatgaag
    16561 cagctgaata tctggagctg aacgttgggg ataactggcg gattagcgaa gggaagccaa
    16621 accctgagct gccaatgcag attttaccgg tgacaatgca agagacggtt tacggcgaac
    16681 tgcactggca aaatagtcac gtttcatcat cagaaccgct gcttaacagt gtttcatcga
    16741 tgctgggacg cggtttgtac tttaatcagg cgcagaagca ttttcagcag ttattgttga
    16801 tggaagaacg tgcgaccatc gcccgcgaat tgcacgactc gctggctcag gtgctttctt
    16861 acttacgtat ccagttgacg ttactgaagc gttcgatacc ggaagataac gccaccgcac
    16921 aaagtatcat ggccgatttt tcccaggcgt tgaatgatgc ttatcggcag ttacgcgagc
    16981 tgttgactac tttccgcctg acgctgcagc aggcggatct cccctccgcg ttgagggaaa
    17041 tgctggatac gttacaaaat caaaccagcg ctaaactgac cctcgactgc cgtctgccaa
    17101 ccctggcact ggatgcgcaa atgcaggtgc atttgttgca aattattcgc gaagcggtgc
    17161 tgaatgcgat gaagcacgcc aacgccagcg aaatcgccgt cagttgcgtc accgcgccgg
    17221 acggcaatca cacggtttat atccgtgata acgggattgg tatcggtgaa ccgaaagaac
    17281 ccgaaggtca ttatggactg aatatcatgc gcgaacgcgc ggaacggctg ggcgggacgc
    17341 tgactttttc acaaccttcc ggcggcggca cgttagtgag tattagcttt cgctccgcag
    17401 agggtgaggg gaaggggaac acgccggctg aggtgtaaat aattaccctc acaccggccc
    17461 tctcccttta aaggcgagag ggaaaaacgt gcccatattg acctgtaagc gagtgtgtca
    17521 gcaatctcat gataatttac attaactcct ttttttctcc acgattggct cgtaccttgc
    17581 cgctacagtg aagcaagtca agcctacaac aatacgcaga aacacgaggt cctcttttaa
    17641 tggcgaattt ctttattgat cgccccattt ttgcctgggt gctgacaatc ctgttgtgtc
    17701 tgacaggtac cctggcgatt ttttcattgc ccgttgaaca ataccccgat ctcgcgccac
    17761 cgaatgtgcg agtgaccgct aactatcccg gcgcatcggc ccagacgctg gaaaacaccg
    17821 tgacccaggt tatcgagcaa aatatgaccg gcctcgataa tctgatgtat atgtcatctc
    17881 agagcagtgg caccggtcag gcatctgtca ctttaagttt taaagcaggc accgatccgg
    17941 acgaagccgt gcagcaagta caaaaccagc tgcaatcagc catgcgaaag ttaccgcagg
    18001 cggtgcaaaa tcagggcgtg acggtgcgta aaaccggcga taccaacatt ctgaccattg
    18061 ccttcgtctc taccgatggc tcgatggata aacaggatat tgctgattat gttgccagta
    18121 atattcagga cccgttaagc cgcgtgaatg gcgtcgggga tatcgatgcc tatggttcgc
    18181 aatattccat gcgtatctgg ctggacccgg cgaaactcaa cagtttccag atgacggcta
    18241 aagatgtcac tgatgccatt gagtcacaga acgcgcagat tgcggttggg caacttggtg
    18301 gtacaccttc cgtcgataag caggcgctca acgccaccat taacgcccag tcactgctgc
    18361 aaacaccaga acagttccgc gatatcacct tgcgggtgaa tcaggacggc tcagaggtaa
    18421 ggctgggcga tgtcgccacc gtcgaaatgg gggcggagaa atacgattat cttagccgct
    18481 tcaatggtaa gccagcctcc gggttggggg taaaactggc ctccagcgca aacgaaatgg
    18541 cgacagcgga gctggtgctc aatcgtctcg acgagctggc gcagtatttc ccgcacggac
    18601 tggaatacaa ggtggcgtat gaaaccacct cgtttgttaa agcctccatt gaagacgtgg
    18661 tgaaaacgct gctggaagct atcgctctgg ttttcctcgt tatgtatctg ttcctgcaaa
    18721 acttccgcgc cacgctgata cccactatcg ccgtgccggt ggtgttgatg ggaaccttct
    18781 ccgtacttta cgccttcggt tacagcgtca acaccttaac catgttcgcg atggtgctgg
    18841 cgatcggtct gctggtggat gacgccatcg tggtggtgga aaacgtcgaa cgtattatga
    18901 gtgaggaagg actcactcct cgcgaagcca cacgtaaatc gatggggcag atccaggggg
    18961 cactggtcgg gattgcgatg gtgctttcgg cggtatttgt accaatggcc ttcttcggcg
    19021 gcaccaccgg tgccatctat cgccagttct ctattaccat tgttgcggcg atggtgctgt
    19081 cagtactggt agcgatgctc ctcactccgg ctctgtgtgc cacgctactt aagccactga
    19141 aaaaaggtga gcatcacggg caaaaaggtt tttttgcctg gtttaaccag atgtttaacc
    19201 gcaacgccga acgctacgaa aaaggggtgg cgaaaattct ccaccgtagc ctgcgctgga
    19261 ttgtgattta tgtcctgctg cttggcggca tggtgttcct gttcctgcgt ttgccgacgt
    19321 cgttcttacc gctggaagac cggggcatgt ttactacctc ggtacaattg cccagcggtt
    19381 caacccaaca acagaccctg aaagtcgttg agcaaatcga gaaatattac ttcacccatg
    19441 aaaaagacaa catcatgtcg gtgtttgcca ccgttggttc tggccctggg ggtaacgggc
    19501 aaaacgtggc gagaatgttt atccgcctga aagactggag cgaacgcgac agtaagaccg
    19561 gcacctcgtt tgccattatc gagcgtgcaa cgaaggcgtt taaccaaatt aaagaagctc
    19621 gcgttatcgc cagcagccca ccagcaatta gcggtcttgg tagttctgca ggttttgata
    19681 tggagttgca ggaccacgct ggagcgggtc acgatgcgct gatggcagca cgtaatcagt
    19741 tgctggcgct ggcggcggaa aacccggagc taacccgtgt gcgccataac ggcctcgatg
    19801 acagtccgca gttgcagatt gatatcgacc agcgtaaagc tcaggcgctg ggcgttgata
    19861 tcgacgatat taacgacaca ctgcaaaccg cctggggttc gagctatgtg aatgacttta
    19921 tggatcgcgg tcgcgtgaag aaagtctatg tgcaggcagc tgcgccgtat cgcatgctgc
    19981 cagatgacat caatctctgg tatgtccgaa ataaagatgg cggcatggtg cccttctctg
    20041 ctttcgcgac ctcacgctgg gaaacaggct cgccgcgtct ggaacgctat aacggttatt
    20101 ctgcggttga gattgttggg gaagccgcac cgggggtcag taccggtacg gcgatggata
    20161 ttatggaatc gttagtgaag cagctgccaa acggctttgg tctggagtgg acggcgatgt
    20221 cgtatcagga gcggctttcc ggcgcgcagg ctccggcgct gtacgccatt tccttgctgg
    20281 tggtattcct gtgtctggcg gcattgtatg aaagctggtc ggtaccgttc tcggtaatgc
    20341 tggtcgtgcc gctgggggta atcggcgcgc tgctggcaac ctggatgcgc gggctggaaa
    20401 acgacgttta cttccaggtg ggcctgttaa cggtcattgg tttatcggcg aaaaacgcca
    20461 tcctgatcgt cgagtttgct aacgagatga accaaaaagg ccatgacctg tttgaagcga
    20521 cgctccacgc ctgccgtcag cgtttacgcc cgattctgat gacctcgctg gcatttatct
    20581 tcggcgtatt gccaatggca accagcacgg gtgccggttc cggtggtcag catgcggtgg
    20641 gtactggcgt aatgggcggg atgatttcag ctactattct ggctatttac ttcgtgccgc
    20701 tgttctttgt gctggtgcgc cgccgcttcc cgctgaagcc gcgcccggaa taagcaataa
    20761 aaaaggcgac atgccaatgt gtcgcctttt tcaactttcc gattaagaac ctgctcagcg
    20821 ggttcttgct gtttatactt tgtctcagga attacttacg aagcataact tcgataaagt
    20881 ctttccagtt ccccagttca cgttcaatca taacaacctc tcttataatt atgggtattc
    20941 tacggaaaca atataccgtg gtgaagctaa tttactcgat tgctgcgatg actacctccg
    21001 ggggacaaac cttatgtaaa tactatggtc ctgcggtgat aatttgtatg tgatacacag
    21061 caacatttcg agatattcat acggcatcta atactgattt aattctggtt aaaata
//
LOCUS       DAZPAB010000056        20712 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZPAB010000056 DAZPAB010000000
VERSION     DAZPAB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.056"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="ACHUPG_001852"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="ACHUPG_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR0861491.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="ACHUPG_001853"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="ACHUPG_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR0861492.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="ACHUPG_001854"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="ACHUPG_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR0861493.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="ACHUPG_001855"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="ACHUPG_001855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR0861494.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="ACHUPG_001856"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="ACHUPG_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR0861495.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="ACHUPG_001857"
     CDS             complement(8217..9110)
                     /locus_tag="ACHUPG_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR0861496.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="ACHUPG_001858"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="ACHUPG_001858"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR0861497.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="ACHUPG_001859"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="ACHUPG_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR0861498.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /gene="ycaQ"
                     /locus_tag="ACHUPG_001860"
     CDS             complement(10241..11473)
                     /gene="ycaQ"
                     /locus_tag="ACHUPG_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR0861499.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="ACHUPG_001861"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="ACHUPG_001861"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR0861500.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="ACHUPG_001862"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="ACHUPG_001862"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR0861501.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="ACHUPG_001863"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="ACHUPG_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="ACHUPG_001864"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="ACHUPG_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR0861502.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="ACHUPG_001865"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="ACHUPG_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR0861503.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="ACHUPG_001866"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="ACHUPG_001866"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR0861504.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="ACHUPG_001867"
     CDS             complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="ACHUPG_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR0861505.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4981 a         5727 c         5031 g         4973 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcaccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatcctca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgcc cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
    18721 ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg
    19861 ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc
    19921 caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt
    19981 caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt
    20041 aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct
    20101 ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag
    20161 cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc
    20221 attcatcatg tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc
    20281 gttgacgtcg ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc
    20341 caatgctttg gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg
    20401 attgccgctg tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct
    20461 taatgttgct gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc
    20521 gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc gcaatcgcca gcagtaattt
    20581 agtattcttc atgtgaatca tccgttgatt aatcatatat aaatattatg tatcaacaac
    20641 ttacattgac aagcgacgct accattaatc aaaatgactc acaaggtccg aaaaaaaacg
    20701 ccgacaatgt gc
//
LOCUS       DAZPAB010000057        20691 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZPAB010000057 DAZPAB010000000
VERSION     DAZPAB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.057"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            81..350
                     /locus_tag="ACHUPG_001868"
     CDS             81..350
                     /locus_tag="ACHUPG_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFR0861506.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="ACHUPG_001869"
     CDS             344..709
                     /locus_tag="ACHUPG_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR0861507.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="ACHUPG_001870"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="ACHUPG_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR0861508.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="ACHUPG_001871"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="ACHUPG_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR0861509.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="ACHUPG_001872"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="ACHUPG_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR0861510.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="ACHUPG_001873"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="ACHUPG_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR0861511.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="ACHUPG_001874"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="ACHUPG_001874"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR0861512.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="ACHUPG_001875"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="ACHUPG_001875"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR0861513.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12379
                     /gene="malT"
                     /locus_tag="ACHUPG_001876"
                     /pseudo
     CDS             9674..12379
                     /gene="malT"
                     /locus_tag="ACHUPG_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="ACHUPG_001877"
     CDS             complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="ACHUPG_001877"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR0861514.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="ACHUPG_001878"
     CDS             complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="ACHUPG_001878"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR0861515.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14849..16447
                     /gene="rtcR"
                     /locus_tag="ACHUPG_001879"
     CDS             14849..16447
                     /gene="rtcR"
                     /locus_tag="ACHUPG_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFR0861516.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16429..17187)
                     /locus_tag="ACHUPG_001880"
     CDS             complement(16429..17187)
                     /locus_tag="ACHUPG_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR0861517.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="ACHUPG_001881"
     CDS             complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="ACHUPG_001881"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR0861518.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="ACHUPG_001882"
     CDS             complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="ACHUPG_001882"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR0861519.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18595..20100
                     /gene="glpD"
                     /locus_tag="ACHUPG_001883"
     CDS             18595..20100
                     /gene="glpD"
                     /locus_tag="ACHUPG_001883"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR0861520.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANALMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20306..20587)
                     /locus_tag="ACHUPG_001884"
     CDS             complement(20306..20587)
                     /locus_tag="ACHUPG_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861521.1"
                     /translation="MQNRKWILTSLVMTFFGIPILTQFFAAVIAMLGAGLAAIPEVCN
                     LLFTPTIYLLLNIFMLTLGALMLFFSGRVGNVPEFCYVGYDGVGFAVIS"
BASE COUNT         4888 a         5692 c         5217 g         4894 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgtat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtc cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cacttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggtt acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    10741 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    10801 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    10861 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    10921 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    10981 acatgaaatc aaggacatca gagaaggcac catgcatgca gaatttaacg ctctgcgcgc
    11041 ccaggtggcg attaacgatg gtaatcagga tgaagcggaa cggctggcaa aactggcact
    11101 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    11161 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctagtgcagc aaaccgaaca
    11221 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaattc agcaaagtga
    11281 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca
    11341 gctgatcaac gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcatccg
    11401 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    11461 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    11521 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagttga accgtctgga
    11581 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    11641 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    11701 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    11761 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    11821 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    11881 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    11941 actggcgaat cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca
    12001 acaactgcgc cagctgattc aacttaatac gctgccggaa ctggaacagc atcgcgcgca
    12061 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    12121 tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctaatccgca ccagcccgtt
    12181 gacccaacgt gaatggcagg tcctggggct gatctactcc ggttacagca atgaacaaat
    12241 tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    12301 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    12361 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    12421 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    12481 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccgtaa
    12541 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    12601 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    12661 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    12721 cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt
    12781 catgcaggga aaaaatcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca
    12841 caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    12901 aggtgctaaa cgatgccacg ggcgagactt ccgttgccac cacaccgcct ccggcaggat
    12961 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    13021 ccagcacccg gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg ccgccgctca
    13081 cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca
    13141 gcgtacaact tccggcgcta ccgatagcaa agcggtaatc gccgccgcgc acggtgccag
    13201 gccggaagac cagacgctgc gatcccagct ccgcgccttc caccgttgcc ctacaaattt
    13261 ccgcagccgc ttttaccgcg gtcagatgct ggcgcagcag ccccggtttc gcccgtccgg
    13321 ctcgaatgcc agtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    13381 gcaggatctg cccgccgcct tcgccctgtg cgccatccag cgcaatcatc cttttcatca
    13441 tttatccttt tacgcacacc acctgacgca gggtgtagat aacttccacc agatcgcttt
    13501 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    13561 catctttacg gcattccaca tgcgcggtgg cacgaatttg gtcttccacg ctgaacagtt
    13621 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    13681 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    13741 catattgacc agcacgcgca gacaccgcac ctttacgcgt cacgtagatc tcttcaccaa
    13801 agagctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    13861 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccaca ttttccatca
    13921 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt aggtaatcat
    13981 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagtt
    14041 ggtcctgcat ctctttttgc gccagatcga taaagtaagt cccgatggcg ttaccaattc
    14101 cgcgtgaacc ggagtgcagc ataatccaca cctgctccga ctcatcaagg cagatttcaa
    14161 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
    14221 gcggatattt ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga
    14281 cgttaaaagg cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc
    14341 cgtgtggtac ggccgtttca atcgcttgac gcagctccgc caggttttca ggcaggtctg
    14401 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    14461 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    14521 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    14581 gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
    14641 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgcggtcct
    14701 gataattgca accgtcgtgc cagaaaatta aataagcaga ttaatttatt gtttctaaaa
    14761 agattatatc tttatatccg taaacggaga tttcccgcaa agccgattta ccgataatga
    14821 aatatcgcct tttataagga tatctaagat gcgtaaaaca gtggcttttg gctttgtcgg
    14881 taccgtgctg gattatgccg gacgcggcag tcagcgctgg tcaaaatggc gtccgtcact
    14941 ctgtatatac cagcaagaat cgttggtcat cgatcgactg gaattattgc acgatgcccg
    15001 ctcgcgctcg ctatttgaaa cgcttaaacg cgatatcgcc agcgtttcgc cagaaacaga
    15061 agtggtgggc gttgagattg aactgcataa cccgtgggat ttcgaagagg tttacgcctg
    15121 cctgcatgat ttcgcccgtg gttacgagtt tcagccagaa aaagaagact atttaattca
    15181 catcaccacc ggcacccacg tcgcgcagat ttgctggttt ctgctggcgg aagcgcgtta
    15241 tctacccgcc cggttgatac aatcttcaac tccgcgcaaa aaagaacagc cgcgcggccc
    15301 aggtgaagtg acgattatcg atctcgattt aagccgttat aacgccatcg ccagccgctt
    15361 tgccgaggaa cggcagcaaa cgctcgattt cctcaaatcc ggtattgcca cgcgtaaccc
    15421 ccacttcaac cgcatgattg agcagatcga aaaagtggcg attaaatccc gcgcgccgat
    15481 tctgcttaac ggcccgaccg gcgcaggtaa gtcatttctg gcgcgacgca tcttcgagtt
    15541 aaaacaggca cggcatcagt ttagcggcgc gtttgtggaa gtgaactgcg ccaccctgcg
    15601 cggcgatacc gcgatgtcga cgctgtttgg tcatgtgaaa ggcgcgttta ccggggcgcg
    15661 ggaatctcgt gaaggattat tacgcagcgc caacggcgga atgttgtttc ttgatgagat
    15721 tggcgaactg ggcgcagacg aacaggcaat gctgctgaaa gctattgaag agaaaacctt
    15781 ttacccgttt ggcagcgatc gccaggtgag cagtgatttt cagcttatcg ccggaacggt
    15841 gcgcgatttg cgccagctgg ttgccgaagg caaattccgc gaagatctgt acgcgcggat
    15901 caatctctgg accttcaccc tgccgggtct acgccagcgc caggaagata ttgaaccgaa
    15961 cctggattat gaagtggagc gccacgccac actcactggt gacagcgtgc gttttaacac
    16021 cgaagcgcgg cgtgcctggc tggcctttgc aacatctccc caggcaacat ggcgcggtaa
    16081 ctttcgcgaa ctttctgcca gcgtcacgcg gatggccacc tttgccacta gcggacgcat
    16141 cactctggac gtggtggaag atgagataaa ccgtctgcgc tataactggc aggagagccg
    16201 cccctccgcg cttacggcgt tgctgggcgc agaggcagaa aacatcgatc tcttcgaccg
    16261 tatgcaactg gaacacgtta tcgctatctg ccgccaggca aagtcgcttt ctgctgccgg
    16321 acgacagctt tttgatgttt cgcgccaggg caaagccagc gtcaatgacg cggatcggct
    16381 acgcaaatac ctggcgcgtt ttggtctgac gtgggaagcc gtgcaggatc agcacagctc
    16441 cagttgaata tggtggtccg tcagcacctg catcacgctt actggcggcg gggcgtcggt
    16501 gtagacggca tcaaccatgc tgatgctccc catattgacc atcgcattac ggccaaattt
    16561 tgaatggtca accaccagca taacgtggcg cgagttctca ataatggcgc gcttggtacg
    16621 aacttcgtgg taatcgaact ccagcagcga gccgtcgctg tcgatgccgc tgatccccag
    16681 aatgccgaaa tcaaggcgga actgggagat aaaatcgagc gtcgcttcgc caatgatccc
    16741 gccatcgcgg ctacgtaatt cgccaccggc gagaataatg cggaaatctt cttttaccat
    16801 caacgtgtta gcaacgttga gattgttggt gacaatgcgc aaattgctgt gattgagcag
    16861 cgcgtgcgcc accgcttccg gcgtggtgcc gatatcgata aacagcgtcg agccattggg
    16921 gatttgctcc gccactttgc gggcgatgcg ctctttttct tcggtctggg tggctttacg
    16981 atcgtgccac ggcgtgttaa ccgaactgga aggcagcgcc gcgccgccat gatggcgcag
    17041 gatcaggttt tgctctgcca gctcgttgag gtcgcggcga atagtctgcg ggctgacgga
    17101 gaagtgctcc accagttcct cggtactgac ataaccctgc tgtttaacca gttcgataat
    17161 accgttgtga cgttgtgttt gtttcattta taaatccctg gaattatttt cgttttcgcg
    17221 cattgagcga atcaacaaaa gccatcgcta aacccacggc taatccggcg atgtgtgctc
    17281 cgttcgccat cgacatccca aacaaatcaa accatccggc gacaatccag atcagcgcaa
    17341 agataattaa cccacgttgc aggtaaatgc cactttgcgg atcgcgttcg ccacgtagcc
    17401 agacgtagcc catcagcgca tacaccacgc cagaaagccc gccaaaccac ggcccgctga
    17461 atttttgctg catatagccg cttaacaggg cgctgataag cgtaatgaca attagcttac
    17521 cgctaccgag gcgtttttcc accgcgccgc cgagatacca ccaccaaagc aggttaaaga
    17581 ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa gtaacgccag aactcaaatt
    17641 tcagtgttgg atcgaatggc caggccagcc ataacatcac ttcctgatcg ccgagaattt
    17701 gcatggcaat aaacaccacc acgcaggcga tcatcatcac ccaggtcacc ggacctgcgc
    17761 gttcacgcaa ggcagcaaag aaaggataac ggcgataatg caggccactg ccggtatggc
    17821 ctgccagcca gctggccgcc agataacgcg gatctgccgg gttttcgaga aaacgcgcca
    17881 gctccgcccg cacgcgctcg gcctgggact catccgccag ccagacatcg ctttggttat
    17941 gttgttgaat cgtgaggata acaccctgcg tcgccatgta atcaacaaac gcctgcgcca
    18001 cgcgggggtt agcaaaagag gtaatcatca acatcgttgc tgtcgcttat tccacacaaa
    18061 aggggacagt ataaagcgtt acgcgccgta cgccacctct gcgggaaact gtcgctgcca
    18121 ggcttcaaag ccgccgtcaa tgctatagac cacatcgtag ccctgttgca gcagatactg
    18181 cgccgcccct ttgctgctat tgccgtgata acacatcacc atcaccggag tgtcaaagtc
    18241 gttatcacgc ataaacgcgc ccagcgtgtc gttggttaaa tggaaagcct gcaccgcatg
    18301 ccccatagcg aaactctgtg gatcgcgaat atcgaccagc accgcctctt tttcctgcaa
    18361 cttctggtgc gcgtcggcaa cgttaataca ttcgaactga tccatgcgtc tctctttctt
    18421 tacaaacaag tgggcaaatt taccgcacag tttacgtcga agcggcagat aaacgccata
    18481 atgttataca tatcactcta aaatgttttt tcaatgttac ccaaagcgcg attctttgct
    18541 aatatgttcg ataacgaaca tttatgagct ttaacgaaag tgaatgaggg cagcatggaa
    18601 accaaagatc tgattgtgat aggtggcggc atcaatggtg ctggtatcgc ggcagacgcc
    18661 gctggacgcg gtttatccgt gctgatgctg gaggcgcagg atctcgcttg cgcgacctct
    18721 tccgccagtt caaaactcat tcacggtggc ctgcgctacc ttgagcacta tgaattccgc
    18781 ctggtcagcg aggcgctggc tgaacgtgaa gtgctgctga aaatggctcc gcatatcgcc
    18841 ttcccgatgc gttttcgcct gccacatcgt ccgcatctgc gcccggcgtg gatgattcgc
    18901 attggtctgt ttatgtacga tcatctgggt aaacgcacca gcctgccggg atcaactggt
    18961 ttgcgttttg gcgcaaattc agtgttaaaa ccggaaatta agcgcggatt cgaatattct
    19021 gactgttggg tagacgacgc ccgtctggta ctcgccaacg ccctgatggt ggtgcgtaaa
    19081 ggcggcgaag tgcttactcg gactcgcgcc acctctgctc gccgcgaaaa cggcctgtgg
    19141 attgtggaag cggaagatat cgataccggc aaaaaatata gctggcaagc gcgcggcctg
    19201 gttaacgcca ccggcccgtg ggtgaaacag ttcttcgacg aggggatgca tctgccttcg
    19261 ccttatggca ttcgcctgat caaaggcagc catattgtgg tgccgcgcgt gcatacccaa
    19321 aagcaggcct acattctgca aaacgaagat aaacgcattg tgttcgtgat cccgtggatg
    19381 gacgagtttt ccatcatcgg caccaccgat gtggaataca aaggtgatcc gaaagcagtg
    19441 aagattgaag agagtgaaat caattacctg ctgaaagtgt ataacgcgca ctttaaaaag
    19501 cagctaagcc gtgacgatat cgtctggacc tactccggtg tgcgtccgct gtgtgatgat
    19561 gagtccgact cgccgcaagc cattacccgt gattacaccc ttgatatcca tgatgaaaac
    19621 ggcaaagcac cgctgctgtc ggtattcggc ggtaagctga ccacctaccg aaaactggca
    19681 gaacatgcgc tggaaaaact gacgccgtat tatcagggta ttggcccggc gtggacgaaa
    19741 gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgcg acgattacgc cgctcgcctg
    19801 cgccgccgtt acccgttcct gactgaatcg ctggcacgtc actacacgcg cacttacggc
    19861 agcaacagcg agctgctgct cggcaatgcg ggaacggtaa gcgatctcgg ggaagatttc
    19921 ggtcatgagt tctacgaagc ggaactgaaa tacctggtgg atcacgaatg ggtccgccgc
    19981 accgacgatg ccctgtggcg tcgcacaaaa caaggcatgt ggctgaatgc ggatcaacaa
    20041 tctcgtgtga gtcagtggct ggtggaatat acgcagcaga agttgtcgct ggcgtcgtaa
    20101 attaacgtaa ggtgatcagg tcagatttca atctggcctg agactgatga caaacacaaa
    20161 actgcctgat gcgctacgct tatcaggcct acgtggttta tgcaatatat tgaatttgca
    20221 tggtcttgta ggccagataa gacgttcacg tcgcatccgg catgaacaaa gcgcactttg
    20281 tcaaaaatct aacctacttt ttaattcagg aaattaccgc aaagcccacg ccatcatacc
    20341 caacgtaaca aaactcaggc acgttcccca ctcgccccga gaaaaatagc attaatgcgc
    20401 ccagcgtcag cataaaaata ttgagcagaa ggtaaattgt tggcgtaaag agtaaattgc
    20461 aaacctcagg aatagcggca agcccagccc ccagcatggc aataaccgct gcaaaaaatt
    20521 gcgtcagtat tgggatgccg aaaaaagtca ttaccagcga ggtcaaaatc cattttctat
    20581 tttgcattat tctttccatt ctttctgaat ggcgaaatta tactccccga gtccccttgc
    20641 cccttctgga cacttttccg aaatgatggc gaaaaaaacg ggaccctttg g
//
LOCUS       DAZPAB010000058        20596 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZPAB010000058 DAZPAB010000000
VERSION     DAZPAB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.058"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(205..1854)
                     /gene="actP"
                     /locus_tag="ACHUPG_001885"
     CDS             complement(205..1854)
                     /gene="actP"
                     /locus_tag="ACHUPG_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR0861522.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1851..2165)
                     /locus_tag="ACHUPG_001886"
     CDS             complement(1851..2165)
                     /locus_tag="ACHUPG_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFR0861523.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2365..4323)
                     /gene="acs"
                     /locus_tag="ACHUPG_001887"
                     /pseudo
     CDS             complement(2365..4323)
                     /gene="acs"
                     /locus_tag="ACHUPG_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            4716..6152
                     /gene="nrfA"
                     /locus_tag="ACHUPG_001888"
     CDS             4716..6152
                     /gene="nrfA"
                     /locus_tag="ACHUPG_001888"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR0861524.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6197..6763
                     /gene="nrfB"
                     /locus_tag="ACHUPG_001889"
     CDS             6197..6763
                     /gene="nrfB"
                     /locus_tag="ACHUPG_001889"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR0861525.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6760..7431
                     /gene="nrfC"
                     /locus_tag="ACHUPG_001890"
     CDS             6760..7431
                     /gene="nrfC"
                     /locus_tag="ACHUPG_001890"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR0861526.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7428..8384
                     /gene="nrfD"
                     /locus_tag="ACHUPG_001891"
     CDS             7428..8384
                     /gene="nrfD"
                     /locus_tag="ACHUPG_001891"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR0861527.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8404..10122
                     /locus_tag="ACHUPG_001892"
     CDS             8404..10122
                     /locus_tag="ACHUPG_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR0861528.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQENSLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10115..10498
                     /gene="nrfF"
                     /locus_tag="ACHUPG_001893"
     CDS             10115..10498
                     /gene="nrfF"
                     /locus_tag="ACHUPG_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR0861529.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10495..11091
                     /gene="nrfG"
                     /locus_tag="ACHUPG_001894"
     CDS             10495..11091
                     /gene="nrfG"
                     /locus_tag="ACHUPG_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR0861530.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11433..12746
                     /gene="gltP"
                     /locus_tag="ACHUPG_001895"
     CDS             11433..12746
                     /gene="gltP"
                     /locus_tag="ACHUPG_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR0861531.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12824..13513)
                     /gene="yjcO"
                     /locus_tag="ACHUPG_001896"
     CDS             complement(12824..13513)
                     /gene="yjcO"
                     /locus_tag="ACHUPG_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR0861532.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13607..15754)
                     /gene="fdhF"
                     /locus_tag="ACHUPG_001897"
                     /pseudo
     CDS             complement(13607..15754)
                     /gene="fdhF"
                     /locus_tag="ACHUPG_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(15952..17418)
                     /gene="mdtP"
                     /locus_tag="ACHUPG_001898"
     CDS             complement(15952..17418)
                     /gene="mdtP"
                     /locus_tag="ACHUPG_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFR0861533.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17415..19457)
                     /gene="mdtO"
                     /locus_tag="ACHUPG_001899"
     CDS             complement(17415..19457)
                     /gene="mdtO"
                     /locus_tag="ACHUPG_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR0861534.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            complement(19457..20488)
                     /gene="mdtN"
                     /locus_tag="ACHUPG_001900"
     CDS             complement(19457..20488)
                     /gene="mdtN"
                     /locus_tag="ACHUPG_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR0861535.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20507..>20596)
                     /locus_tag="ACHUPG_001901"
     CDS             complement(20507..>20596)
                     /locus_tag="ACHUPG_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861536.1"
                     /translation="RANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4618 a         5450 c         5641 g         4887 t
ORIGIN      
        1 catattattt aaaccattat agcgcataaa atatccttat aattaacaag aaagaaaagg
       61 catattctct gcattatcat tttctacgac tgtcaaaaat cgctcatttt ttaatgagtt
      121 tatttgttta atattatggg aaaaggtgat gcatgttgga gaggaagcgt attccccggt
      181 caggcgaccg gggaagggga gagattaatg tgcgcggcct tgctcaacgc caaagccggt
      241 ctgggagcgg ataaactgcg cgcggaacag ttcacgctcg cgcgcccctt ccgctgagtt
      301 atcggttgcc gagaagaacc agatgccgag gaatgccacg ctgatagaga acagcgccgg
      361 gtattcatac gggaagatgg ctttttcgtg accgaggatc tgtacccaaa tagtcgggcc
      421 gaggatcatc agcaccactg cggtaatcag ccccagccag ccgcccagca tcgcgccacg
      481 ggtagtcagt ttcgaccagt acatggaaag cagaatgatc gggaagttac agctcgccgc
      541 gatggcaaac gcaaggccta ccataaaggc gatgttctga ttctcaaaca gcacgccgag
      601 gataatcgca atcacgccga ggatcagtac ggtgattttt gatacccgca gctcttcacg
      661 ttcggtcgcg ccttttttga agacgttagc gtacaagtca tgcgaaaccg ccgatgcgcc
      721 cgccagcgtc agatccgcaa ccaccgcgag gatagtggcg aaagcaaccg ctgaaataaa
      781 accgaggaat aggttgccgc ccactgcatt cgccaggtga acggccgcca tgttgttacc
      841 accaatcaga tggcccgccg cgtctttata ttccggattc gcaccaacaa gcatgatcgc
      901 gccgaagccg ataataaagg tcagaatata gaagtagccc ataaatccgg tggcgtagaa
      961 cacgctctta cgtgcttcgc gggcatcgct gactgtaaag aagcgcatca gaatgtgcgg
     1021 caagcccgcc gtaccaaaca tcagtcccag ccccagagag agcgcggaga tcggatcttt
     1081 caccagtccg cccggtttca tgatgtcgac acctttcggg tgtaccgcca tcgcttcgct
     1141 gaacagattg ttgaagctaa agccgacgtg tttcatcacc ataaaggcca taaagctggc
     1201 accgaacagc aacagcacag ctttgataat ttgtacccag gtggtcgcca gcatgccgcc
     1261 gaacaggacg tacatcatca tcagcacgcc gaccagcacc accgcaatgt gatagttaag
     1321 gccaaacagc agctcgatca gtttacctgc gcctaccatc tgagcgataa ggtaaagcgc
     1381 caccactacc agagaaccac aggccgaaag aatacgaatc ggcccttgtt tcagacgata
     1441 agaggccaca tcggcagagg tgtagcgtcc caggttacgc agacgttcgg caatcagaaa
     1501 cagaatgatt ggccagccca ccaggaagcc caacgagtag atcagcccgt catagccgga
     1561 ggtaaacacc agcgcggaga tccccaagaa tgaggcggcg gacatatagt ccccggcaat
     1621 cgccagcccg ttctggaagc cagtgatatt gccgcctgcg gtgtagtagt cgctacgaga
     1681 acgtacgcgt tttgatgccc agtaggtaat gccgagcgta aacacgacga aaatcaggaa
     1741 cataataatc gcctgccagt tcgttggctg gcgctctacg gccccgctaa tagcatccgc
     1801 ggcgttagct gcgaaaggga gtgtggcggc aagcgccgtc agaactcttt tcatgatgct
     1861 tgtacctcat gcaggacttc attattaaga cggtcgaatt cgccgttcgc ccgccagatg
     1921 tagataccgg tgagaacaaa ggagatcaca atcactccaa caccaattgg aataccgcgt
     1981 gtgacgctgg tgttcggatt cagcggcgtg cccagccagc cgggcgcgaa ggcgatcagt
     2041 aaaataaagc cgatataaac tgccagcata ataatcgaca ggatggtggc aaaccgttgc
     2101 cgtttttcga ctaactccct gaaatgcgca ttgtcttcta tccgctgata aatcgtacca
     2161 ttcatcacag attctccaga ggtaatgtag ggattgtttt aattcccgct cccttatggg
     2221 agaaggttaa cgctcgggta acccttgccg aatgtaggcc ggataaggcg tttacgccgc
     2281 atccggcaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac aaaccgttac
     2341 cgattcgcat ccggcaattg tgggttacga tggcatcgcg atagcctgct tctcttcaag
     2401 cagcttctcg accacgccag gatcggcaag cgtcgaggta tcgcccaggt tgctggtatc
     2461 gcccgccgca attttgcgca gaatacggcg cataattttg ccggagcggg ttttaggcag
     2521 ggagtcggtc cagtgcagca cgtctggcgt cgccagcggg ccaatctctt tacgcaccca
     2581 gttgcggact tctgcgtaca gttctggtga cggttcctcc ccgtgattaa gcgtgacata
     2641 ggcgtagatc gcctgacctt taatattgtg cggaatacct actacggcgg cttcggcaat
     2701 cttcggatgc gccaccagcg ccgactctat ctctgccgtc cccagacggt gaccggagac
     2761 gttcagcacg tcgtccacac gcccggtgat ccagtaatag ccatcttcat cgcgacgcgc
     2821 gccgtcgccg ctgaaataca tatttttgaa ggtggaaaaa taggtctgct caaaacgttc
     2881 gtgatcgcca aacagcgtac gcgcctgacc aggccaggag tcggtgatca ccaggctacc
     2941 ttcggtagcc ccttccagcg ggttaccttc gttatcgacc agcgccggtt gcacgccgaa
     3001 gaacggacgt gttgccgaac cggctttcag ctcggtagcg ccaggcagcg gggtgatcat
     3061 gaaaccgccg gtttcggtct gccaccaggt atcgaccacc ggacatttct cgttgccgat
     3121 ttttttccag taccactccc acgcttccgg gttaattggc tcgcccacgg aaccgagaat
     3181 gcgtagcgac gaacggtcgg tgccttcaat cgctttatcg ccttccgcca tcagcgcgcg
     3241 gatcgccgtg ggtgcggtat agagaatatt gacctgatgc ttgtccacca cctgcgccat
     3301 acgggcaggc gtcggccagt tcggtacgcc ttcaaacatc agcgtggttg cgccgcaggc
     3361 cagcgggccg tacagcaaat aactgtgtcc ggtaacccag cccacatcgg cggtgcacca
     3421 gtagatatca cccggatgat aatcaaagac atatttaaag gtcagcgccg cgtacaccag
     3481 ataaccgccg gtagtgtgca gtacgccttt tggttttccg gtagaaccgg aggtatagag
     3541 aataaacagc ggatcttcgg cgttcatctc ttccgcctag tgctgatcgc tggcttgctc
     3601 aaccaggtcg tgccaccaca ggtcgcgccc ttcctgccag tcgatgtttc cgccagtacg
     3661 cttcagtacc accacatgct ctacgctggt gacgttcggg ttttttagtg cgtcatcaac
     3721 gtttttcttc agcggaatac tacgcccggc gcgcacgcct tcgtcggaag tgatcaccag
     3781 tcgtgagttg gaatcaataa tgcgcccggc aacggcttcc ggcgagaagc cgccgaaaat
     3841 caccgaatgc atcgcgccaa tgcgggcgca ggccagcatc gcaaccgcgg cttccggcac
     3901 catcggcata taaatcgcca ccacatcacc ttttttaatg cccagctcga gcagggtatt
     3961 ggcgaagcgg cagacgtcgc ggtgcagctc tttatagctg atatgtttgc tctggctggc
     4021 gtcgtcgcct tcccagatga tggcggtgcg atcgccgttt tcttgcagat ggcggtcaag
     4081 gcagtttgcc gccagattca gcgtgccgtc ctcgtaccat ttaatggaca cattaccggg
     4141 ggcaaaggag gtatttttca ccttctggta cggtttgatc cagtcgagaa tttttccctg
     4201 ttcgccccag aaggtatcag gtacgttaat agattgttga tacatcgcct cgtactgctg
     4261 agggtttatc aggcaacggt ctgcgatgtt ggcaggaatg gtgtgtttgt gaatttggct
     4321 catgcttttg ttctccttgt aggatgttaa taatatgtgg cataagcgtt aaatgtaggg
     4381 gtattggcag ttttgtttag tatttgggcg acagatcacg caaaagtaga atggtgcaaa
     4441 taaacggcag ggtaattttt gaaggtcacc aacaaaagtt gattaattct ttgaggaaca
     4501 tgcagttatg catgctgttg aaaaagagga agatactgac taactctaaa gtggtatttc
     4561 acatgcactt acaattgatt aaagacaaca ttcacagtgt ggttatttgt tacacatagg
     4621 ggtgagcaat gtcatgacag tgtaggtgcg gttactgtcg tgaaaaacaa taaaaaccgc
     4681 cattgcaaca atggcgcaat tcggatgaag cccctatgac aaggataaaa ataaacgcac
     4741 gccgtatctt cagcttattg attccttttt tctttttcac ttctgttcac gctgaacaaa
     4801 cggcagctcc cgcaaaacct gtaactgtgg aagcgaagaa tgaaaccttt gccccgcagc
     4861 atcacgatca atatctctcc tggaaagcca cctcggaaca gtcagagcgt gttgacgccc
     4921 tggcggaaga tccacggctg gtgatcctgt gggcggggta tcccttctcg cgcgattaca
     4981 acaagcctcg tggacatgcc tttgctgtga ccgatgtgcg tgaaaccctg cgtaccggtg
     5041 cgccgaaaaa cgctgaagat ggtccgctac cgatggcgtg ctggagttgt aaaagcccgg
     5101 atgtggcgcg tctgatccag aaagacggcg aagatggcta cttccacggt aaatgggcgc
     5161 gcggcggccc ggaaatcgtc aacaacttag gttgtgccga ctgccataac accgcctcac
     5221 cagagttcgc caaaggcaaa ccggagttaa ccctttcccg tccgtatgcg gctcgcgcga
     5281 tggaagccat tggtaaacct tttgagaaag ccggacgttt cgaccagcaa tcgatggttt
     5341 gcggtcagtg ccatgtggag tattacttcg acggcaaaaa caaagcggtt aaattcccgt
     5401 gggatgacgg catgaaagtc gaaaatatgg agcagtatta cgacaaaatt gccttctctg
     5461 actggactaa ctccctgtcg aaaacgccaa tgctgaaagc gcagcacccg gaatatgaaa
     5521 cctggacagc gggcattcac ggtaaaaaca acgtgacctg tatcgactgc catatgccaa
     5581 aagtgcagaa cgccgaaggc aaactctaca ccgaccataa aattggtaat ccgtttgata
     5641 acttcgccca gacttgtgcg aactgccata cccaggataa agctgccttg caaaaagtgg
     5701 tcgcggaacg taagcaatcg attaacgacc tgaaaatcaa ggttgaagat caactggttc
     5761 acgctcactt cgaagcgaaa gcggcgctgg atgcaggcgc gacggaagcc gaaatgaagc
     5821 cgattcagga cgatatccgt catgcccagt ggcgttggga tctggcgatt gcttcccacg
     5881 gtattcatat gcacgcaccg gaagaaggtc tacggatgct cggtacggcg atggataaag
     5941 cggcggatgc gcgcaccaaa ctggcgcgcc tgctggcgac caaaggtatc acccatgaaa
     6001 tccagatccc ggatatctcg accaaagaga aagcccagca ggccattggc ctgaacatgg
     6061 aacaaatcaa ggccgagaag caggacttca tcaaaacggt gatcccgcag tgggaagagc
     6121 aggcacgtaa aaacggtctg ttaagccaat aaccccgttc cgcctcgcaa ggggcggaaa
     6181 acacaatgga gtgaatatga gcgtattacg ttcgttatta actgctgggg tgctggcgtc
     6241 gggcctgttg tggagcctga acggaattac cgctacccct gcggtgcagg catctggtga
     6301 tcgctacgaa gttacccagc agcgtaaccc ggatgccgcc tgtctggact gtcataaacc
     6361 agataccgaa ggtatgcatg gcaaacatgc ttccgttatc aacccgaata acaaattgcc
     6421 ggtcacctgc accaactgtc acggccaagc atcaccgcaa caccgcgaag gggtgaaaga
     6481 tgtgatgcgc tttaacgagc cgatgtataa ggtcggagag cagaacagcg tctgtatgtc
     6541 ctgtcatctg ccggaacagt tgcaaaaagc gttctggccg cacgatgtcc acgtaaccaa
     6601 agtggcgtgc gccagctgcc attccctgca tccacagcaa gatacgatgc agacgttaag
     6661 tgacaaagga cggattaaga tttgcgtcga ttgccacagc gatcagcgca ccaatccgaa
     6721 ctttaacccg gcgtctgttc cgttgcttaa ggagcagcca tgacctggtc tcgtcgccag
     6781 tttctcaccg gcgtcggcgt gctggcagcc gtcagcggca ccgcagggcg cgtggtggcg
     6841 aaaacgttga atatcaatgg ggtgcgttac ggcatggtgc atgacgagtc gttatgcatc
     6901 ggctgtaccg cctgtatgga tgcttgtcgg gaagtgaaca aggtgccgga aggcgtctcg
     6961 cgcctgacga ttattcgtag cgagccgcag ggcgaatttc ctgatgtgaa atatcgcttc
     7021 ttccgtaagt cttgccagca ctgcgatcat gcgccgtgcg ttgacgtctg cccgaccggt
     7081 gcgtcttttc gtgatgccgc tagcgggatt gtcgatgtta atcctgatct ctgcgtcggt
     7141 tgtcagtact gcatcgccgc ctgtccgtac cgcgtgcgct ttatccatcc ggtcacgaag
     7201 acggcggaca aatgcgattt ctgtcgtaag accaatttgc aggccggtaa gcttcccgcg
     7261 tgcgttgaag cttgcccgac gaaggcgctg acgtttggca atctggacga tcctaacagt
     7321 gagatttcgc aactgctgcg ccagaagcct acttaccgct acaagctggc gctgggaacc
     7381 aaaccgaagc tgtaccgcgt accgtttaaa tacggggagg tgagccaatg acgcagactt
     7441 ccgcatttca ttttgaatcg ctggtgtggg actggccgat tgccatctac ctgtttttga
     7501 ttggtatttc tgccggtctg gtgacgctgg ccgtgctgtt acgtcgcttc tacccgcagg
     7561 cgggcggtgc agacagtacg ttgctgcgca ccacgctgat tgtcgggccg ggcgcggtga
     7621 tcctcggtct gttgatcctc gtcttccacc tgacaagacc gtggaccttc tggaagctga
     7681 tgttccacta cagttttacc tcggtgatgt cgatgggggt gatgctgttt cagctctaca
     7741 tggtggtgct ggtgctgtgg ctggcgaaaa tctttgaaca tgatttgctt gccctgcaac
     7801 aacgctggtt gccgaagctg gggatcgtgc aaaaggttct gagcctgctg acgcccgttc
     7861 atcgcggact ggaaacgctg atgctggtgt tggcggtgct gctgggggct tataccggct
     7921 ttctgctgtc ggcgctgaaa tcgtatccgt tcctcaataa cccgagcctg ccggtgctgc
     7981 tcctcttctc cggcatctcg tccggtgcgg cggtggcgct gatcgccatg gcgatacgcc
     8041 aacgcagtaa cccgcattcc acggaagcgc actttgttca ccggatggaa atccccgtgg
     8101 tatggggtga aatcttcctg ctggtggcgt tttttgtcgg tctggcgctg ggcgatgacg
     8161 gtaaagttcg tgcgctggtg gcggcattag gtggcggttt ctggacgtgg tggttctggc
     8221 ttggtgtcgc cgggctgggg ctgattgtgc caatgttgct caaaccgtgg gtcaatcgca
     8281 gttccggcat tcctgccgtg ctggcggcgt gtggggccag tctggtcggc gtgttgatgc
     8341 tgcgcttttt cattctctac gccggacagt tgacggtggc gtaagccaga aaagaggtgg
     8401 tttctggacg tattccttcc tgaagtcggt tttctggcgt tgttgttaag tctcggggtc
     8461 aacgtgttga ccccgttgac ggcctttgcg ggagtgcggt tgcgctggcc tgccatgatg
     8521 cgactcactt gcatcggcat tctggcgcag ttcgcgctcc tgctgctcgc ctttggcgta
     8581 ctgacgtatt gttttctcat cagcgatttc tcggtcattt atgtcgccca acatagctac
     8641 agcctgctgt cgtgggaact caaactggcg gcggtgtggg gcggtcatga aggttcgctg
     8701 ctgctttggg tgctgctgct ttccgcctgg agcgcgctgt ttgcctggca ttatcggcag
     8761 caaagcgatc cgctatttcc gctgacgcta gccgttttat ctctcatgct cgccgcactg
     8821 ctactgtttg tggtgctgtg gtccgatccc ttcgtgcgga tatttccacc agcaatcgaa
     8881 ggccgcgatc tcaatccgat gctgcaacat cccggtctta tctttcatcc accgctgctt
     8941 taccttggct atggcggttt gatggtagcg gcgagcgtgg cgctggcgag tctactgcgc
     9001 ggcgagtttg atgcggccag cgcccgaatt tgctggcgct gggcgttacc tggctggagt
     9061 gcattaacgg cggggatcat cctcggttcc tggtgggcct actgcgaact cggctggggc
     9121 ggctggtggt tctgggatcc ggtggaaaac gcctctttat taccctggct ttctgccact
     9181 gcgctgctgc acagtttgtc cctgacacgc cagcggggga ttttccgcca ctggtcgctg
     9241 ttgctggcga tagttactct gatgctgtcg ctgctgggca ccttaattgt ccgttctggc
     9301 attctggttt cggttcatgc gttcgcactg gataacgtcc gcgccgtgcc gttgttcagc
     9361 ctgtttgcac tgattagcct tgcgtctttg gctctgtatg gctggcgagc gcgggacggt
     9421 ggcccggcgg tgcgtttttc ggggttatcg cgggaaatgt taatcctcgc tacgctgttg
     9481 ctgttttgcg cagtgctact gatcgtgctg gtgggaacgc tttatccgat gatttacggc
     9541 ctgctgggct ggggacgcct ctccgttggc gcgccgtatt ttaaccgcgc gacgttaccg
     9601 tttggtctgt tgatgctggt ggtgattgtg ctggcgacgt ttgtctctgg caaacgcgtg
     9661 cagcttccgg cgctggtagc tcatgctggc gtgctgttat ttgccgcggg gatcgtggtt
     9721 tccagtgtca gtcgtcagga gaacagcctg aatttacagc cgggtcagca ggtgacgctg
     9781 gcaggataca ccttccgttt tgagcgactc gatctgcaag ccagaggcaa ttacaccagc
     9841 gaaaaagcga tagtggcgct gtttgaccat cagcaacgca ttggtgaatt aacgccggag
     9901 cggcgttttt atgaagcacg ccgtcagcaa atgatggaac cgtcaattcg ctggaacggc
     9961 atccatgact ggtatgcggt catgggtgaa aaaaccagag cggatcgtta cgcttttcgt
    10021 ttgtatgtac aaagcggtgt gcgctggatc tgggggggag gattgttgat gattgcgggc
    10081 gcattgttaa gcggatggcg ggggaagaag cgcgatgaat aaagggcttc tcacgctact
    10141 gctgttattt acctgttttg cgcgcgctca ggttgtagac acctggcaat tcgccaatcc
    10201 gcaacaacag caacaggcgt taaatattgc cagccagtta cgttgtccgc agtgccagaa
    10261 tcaaaactta ctcgaatcca atgcgccggt ggcggtcagt atgcgccatc aggtttatag
    10321 catggtgtcg gaggggaaga acgaagtcga aatcattggc tggatgaccg aacgctacgg
    10381 agattttgtt cgctataacc caccgttaac gggtcagacg ctggtgttgt gggcgctgcc
    10441 agtggtgttg ttattgctga tggcactgat cctctggcga gtgagggcta agcgatgaaa
    10501 cagcctcaaa taccggtgaa aatgctgaca acactcacga tattgatggt attcctctgt
    10561 gtcggcagtt atctgttaag cccaaaatgg caggcggtac gtgcggagta tcagcgtcag
    10621 cgcgatccgc tacatcagtt tgccagccag caaaccccgg aagcgcagct tcaggcattg
    10681 caggataaaa tccgcgctaa tccacaaaac agcgaacaat gggcgttact gggcgagtac
    10741 tatctgtggc aaaacgatta cagcaattcg ctgctggcgt atcgtcaggc gttgcaacta
    10801 cgtggtgaga acgctgaact gtatgcggcg ctggcgacgg tgctttatta ccaggccagc
    10861 cagcatatga ccgcccagac tcgcgcaatg atcgacaaag ccctcgcgct ggactctaac
    10921 gaaatcaccg ctctgatgct gctggcttcc gatgcgttta tgcaggcgaa ttacgcgcaa
    10981 gccatcgaac tatggcaaaa agtgatggat ctcaactcac cccggatcaa ccggacacag
    11041 ctggtcgagt cgattaatat ggcaaaactg ttgcagcgga gatcggatta atcatctggt
    11101 gatgaagatt atgtcattga gctgcataaa aaataatcga atgaacatat gccaaaaata
    11161 atcaccaatc agtattattg cagattaaac aaataaaaat ctttccataa caaatggtta
    11221 ttcattaatc ctgccataaa ctcgcgttat tatgcattaa tgcagcgaaa agctctgttg
    11281 ttaaagggtt gcgcaacata ccgcgcaaat gatactgatc ataagcgtta aaaaaatcta
    11341 caaaccaacg caacacaatt catgccctgg cagtatgtca cgttctcgcg tttctgaacg
    11401 gggaacggcg ctccattgag gaagttattc tgatgaaaaa tataaaattc agcctggcct
    11461 ggcagattct gtttgctatg gtgctgggca ttctcctggg aagctacctg cactaccata
    11521 gcgacagccg cgactggctg gtcgtcaatt tgctctctcc ggcgggtgat atcttcatcc
    11581 atctgatcaa aatgattgtt gtgccgattg tgatctccac gctggtggtg ggtatcgcgg
    11641 gtgttggtga tgccaaacag ctcgggcgta ttggcgcgaa aaccattatt tacttcgagg
    11701 tgatcaccac cgtcgccatc attttgggga tcactctggc gaacgtcttc cagcccggtg
    11761 ccggggtgga tatgtcgcag ctggcgaccg tcgatatctc gaaatatcag agcactacgg
    11821 aagcggtaca aagcagttcc cacggcatta tgggcacgat tttgtcgctg gtgccgacga
    11881 acattgtggc gtcgatggcg aaaggcgaaa tgctgccgat catctttttc tcggtgctgt
    11941 ttggtctggg gctttcttcc ctgcccgcga cgcatcgtga accgctggtg accgtgttcc
    12001 gctccatctc tgaaaccatg tttaaagtga ctcacatggt gatgcgttat gcaccggtgg
    12061 gtgtgtttgc gctgattgcg gtgacggtgg ctaactttgg tttctcgtct ctgtggccac
    12121 tggcgaaact ggtgctgctg gtgcatttcg ccattctgtt cttcgcgctg gtagtgctgg
    12181 gaattgtggc acgtctgtgc gggttaagcg tctggatcct gattcgtatt ctgaaagatg
    12241 agctgattct ggcgtactcc actgccagct ctgaaagcgt actgccgcga attattgaga
    12301 agatggaagc ctacggagca ccggcgtcga tcaccagctt cgtggtgccg accggttact
    12361 cttttaacct tgatggttcg acgctgtatc aaagtattgc cgctattttc atcgctcagc
    12421 tgtatggcat tgacctgtcc atctggcagg aaatcattct ggtgctgacg ctgatggtga
    12481 cctcgaaagg gattgctggc gtggctggcg tgtcgtttgt ggtgttgctg gcaacgctgg
    12541 gtagcgtagg tattccgctg gaaggtctgg cgtttattgc tggtgttgac cgtatcctcg
    12601 acatggcgcg tactgcgctg aacgtggtgg gtaatgcgct ggcggtgctg gtgattgcca
    12661 agtgggaaca caaatttgac cgcaaaaagg cgctggctta tgagcgtgaa gtgctgggca
    12721 aatttgataa aactgcggat caataattga agattgccgg ggatatcccc cggcaatgtg
    12781 tggatgcctg atgcgacgct tgccgcgtct tatcaggcct accttaaccg ttggttaatt
    12841 tttcaaactc ttcacacccg gtatcaaacc cttccataca gctcaggttc aaccagtgca
    12901 gcgccttttg tttgttcttc tcgataaaac cttcctcacc gtttaagaac atcattcccg
    12961 cccagtactc ggaataaccg gttcgggaaa ttgcagagct gcgtttgaaa taccaggttg
    13021 ccttgtcatc gtcggcctta atgcccacgc cgttggcgta aatcaaacca agcagcattt
    13081 gggcatcgac ggcggagtca ttctccagat cttccgaggc gttttctaac agcgaaatcg
    13141 cctttggata atccggttta cccgcttgag tatttaccag aatatgcgcc agcgttactt
    13201 caccttcttt actgcccgct tgcgccgctt tctctgcaag aacttttgct tgtggatagt
    13261 ccagactgac cggattggtg attttgatct gcgccaacag cgcgcaggca tcagcatcac
    13321 cgctgtcggc ggctttctgt gcccaatatt cggctttgct caaatcgccg gagctaaacc
    13381 agctgtcggc aagaaaatat tgtgcacgtc ggtccccggc ctctgctgcc tttaaatact
    13441 ggctgccagg ctcggagtcg ttcgcgtggg caaagaatgt caaaaacaac atcaatgcga
    13501 taattttttt cattttgata atacgattgg gtagggagta accagtataa agagttcgaa
    13561 tggataaaaa aacagcctcc gaaaggaggc tgtagaaagg actgtattac gccagtgccg
    13621 cttcgcgcag gcgagttttc aacttgttgt actcgtcaat cacgtactgc tcggcggcgc
    13681 gctgatcgga gatcggctcg acgcgcacgg cgcagtattt gtattccggc gttttcgtaa
    13741 tcgggcttaa gctttcggta accagctcgt tacaggcacc aatccaccac tggtaggtca
    13801 tgtaaatcgc ccctttgttc ggacgatcgc tgacctgcgc acgggtgata attttgcctt
    13861 tacgcgagtg cactcaaacc aatgcctcat cttcaatacc cagacgtttg gcgtcttcgg
    13921 tattgatttg tgcgtagcca ggttcatcag ccagcgccgc cagtgccgca cagttaccgg
    13981 tcatcgaacg gcaagagtag tgaccaactt cacgcaccgt tgacagtacc atcgggtact
    14041 cgtcggtgag tttgtcgatt ggcgctaccc agtcgcaggt gaagaactgc gccagaccgt
    14101 tcggggtatc aaacttctct ttaaacagat aagaagtccc ctgatcggca tctgaagtat
    14161 cgcggcaagg ccactgaatg aagcccagtt cgcccatttt ctcgtaagtc gcaccgtaga
    14221 aatccgggca cagatgacgc aactcatccc agatctcctg ggtgttgttg tagtgcatcg
    14281 gataacccat acgggtggcg atttcactga tgatttgcca gtccgttttc agatcccatt
    14341 tcggttcaac tgctttgaag aaacgctgga agccacggtc agccgcagta aacacgcctt
    14401 catgctcgcc ccacgacgtt gacggcaaaa tgacatccgc cgccgacgcg gttttggtta
    14461 taaagatgtc ctgaacgatg accagttcca gatcttcaaa ggctttacgt actgccgaca
    14521 gctccgcatc agtttgcagc ggatcttcgc ccataatgta cgcggcacgc acttcgccat
    14581 gcgctgcgcg gtgcggcagc tcgctgatgc gatagccggt atgagcaggc aggctttcca
    14641 cgccccaggc tttggcgaat ttttcgcggt tagccggatc tttcacgtac tggtatcccg
    14701 gatacgtatc cggcagcgcg cccatatcgc aggcaccctg cacgttgttc tgaccacgaa
    14761 ccgggtttac acccgcatgc ggcttaccga ggttactggt cagcatcgcg aggctggtca
    14821 gagaacgcac ggtttccacg ccctggtaga actgggttac acccatgccc cacaggatgg
    14881 cggcgctttt cgcctgggca tacatccgtg ccgcctgacg aatctcactg gcgctgacac
    14941 cggtgatatc ttcaaccgac tccggcgtgt agccttcaac gattttacga tactcttcaa
    15001 agccttctgt acgggaagcg acgaacgctt tgtcgtacag attttcttca ataatgacat
    15061 ggcccatcgc attcaacagc gcgatgttcg agccgttttt cagtgcaatg tgcatgtcag
    15121 caatgcgcgc ggtttcaatt ttgcgcggat cgcagacgat aattttcgcc ccgttacgtt
    15181 tagcgttaat tacgtgattc gccacgattg ggtgggaatc cgccgggttg tacccgaaaa
    15241 cgaacactaa atcggtatta tcaatttcgt taatagcatt gctcattgcg ccattaccga
    15301 ccgattggtg cagacctgca accgatgggc cgtgtcagac acgagcgcag cagtcaacgt
    15361 tattggtacc aataacggcg cgcgcaaatt tttgcattac atagttggtt tcgttacccg
    15421 taccacgcga ggagccggtc gtctggatgg catccggacc gtacttctct ttgatggcgc
    15481 tcaggcgctc ggcaacgtaa ttcagtgcct catctcagga aacaggttcg agtttgccgc
    15541 cacgctggcg acggatcatg ggggttttca ggcgcggggt caggatctgg gtatcgttaa
    15601 tgaaatccca gccataataa cccttcagac acagggtacc ctggttggtt ttcccctgcg
    15661 ctgcctccgc ccggacgatt ttgccgttat cgacgaccag gttgattttg caacctgatg
    15721 cgcaataggg gcaaaccgtg acgacttttt tcatcggtct cgctccagtt aatcaaatca
    15781 cgcatacgcg ctctcgacta cagtatgcat cttttatgcc acattttatt tggggtcatt
    15841 ccctgatatt acgggcacta tttattcaaa actctgacga aaaacaggct gtcgtcagtt
    15901 ttgacgtgac gaaacgaaat accgcgtgac agccatcacg cggcagacat tttatttttt
    15961 ctcgacgacg ggagccgcct gatacccgcc gcccagcgat ttcatcaact gaatgctttg
    16021 gatcacccgg cggctgtcca gcatcagtaa tgacatctct tcggcaagta ctggcaaccg
    16081 ggcttcagtg gcctgtaagc ggctggttaa gccgcgctga taggcggcct cggcagcgcg
    16141 ctgggtgaag cgcgttgctt ccacgtgttc agcctgcatt tctcgctcgt cgttgagcgt
    16201 ttgcagacgc gtgccgttga cggcaacgtc acgcaccgcg ttcagtactg actggttgta
    16261 acgttcaatc atcatgttgc tggcggcgcg cgtgccttcg agattggcat tcaaccgtcc
    16321 accgtcaaac agcggcaatt tcagacccgg gatgaagttg aactggcgac tggttttttt
    16381 gaataaggta tccaggtgga tggcatcaag gccgaaaaac gctttgatat caaagctcgg
    16441 atagaacaac gcccgcgcgg aatccacctg atctaatgac gcctgaacat accagcgcat
    16501 ggcttgcaga tccgggcgtc tggcgagcaa ctcataagag agtgtcgccg gaatgccggt
    16561 ctggactcgt ggtaatgcca caggtttgat ctccggcata tcgctggctc ccgcgccaat
    16621 caatgcgcgc agagattctc tcgtttcggt gatttgccct ttgacggcag caatttgttt
    16681 atcgaccgcc agaatctgtg cccgcgcgcc gtggaaaggc acttgtgctt ccagaccgtg
    16741 cgccacttta ctttggtgcg ctttcaccgc gtaatcaatc acatcgcgag tttgttctaa
    16801 cagatcgagc atctgatagc tggcctgcat actgtaataa agctgcgcta cgcccgtggt
    16861 cagcgatagc tctactgctg cggtttctgc cagcgcggca ttatgcgcgc caatggcggc
    16921 ggcaaccgct gagcgatgca caccccacaa atcaagatcc agtccggcaa acaaacctac
    16981 tgtggcttcc gtatagtacg gcccgtccat ccccagtgcg ggcgcatcca tcgcataagg
    17041 gcttaaaaag ccgttcgccg agacacgttg gcggttaagc atccctaacg ctgccacctg
    17101 caactgtgaa ccggcatcta acaaatcggc ctgcgattgc gctttttctt cccgcagttt
    17161 cgcttcggcg agggtgtgtg aaccacttag cgtccgttgg atcagggcat ccaactgcgg
    17221 gtcattgagt tgtttccacc actgcgcctg cggccagccg gagctggcaa gatgaatatc
    17281 atcggccagt ttgatttgtt ccggtttgag ctgttgatga ggcgcagaat ccttacgtac
    17341 cagggcacag ccggaaatca gcgtcgtgct gccgagaatg ctgcacaaca gcagacgtga
    17401 aagttgacga ttgatcattg cgtggctcct tgtgcctgtt ccgtggcggg cgttaatgcc
    17461 ggggctgtcc agtctggcaa gcgggcgaaa agctggcaga cgtgttgctc ctgttttaat
    17521 aaggtgggca ggatggcctg cgaggtgggt gtctcttcaa gcgttatttg aggagaacgg
    17581 ctgagtgcgg ttgccagacc ggcagcgtat ttctccaggg cgtcggcaaa ctgacctgct
    17641 ctgtccggct gtagtgcgtt atccagcgcc tgcgccgaat tccaggtggc atcccaggcg
    17701 tgaaggatat cgcggccctg acgaataacc gtttgcgaac gttcaatcag taaggcgcgt
    17761 tcttcgctgt ccagttgacg ctccagcgcc acgcgttggc acatctcttc acaggcatta
    17821 aacgccgcat gcagaccgat acgaatttgc agataagtgc gcagagccgt gacttcctgc
    17881 tggcgtggga tccgcattac tttacttaac atacccagcg cgccagccag tttttgcggc
    17941 agcgtgcgcg cttcactttc aggccagaca aaggtgtaaa tcatcgcgga caccacggta
    18001 ccaatgatga tacccagggc gcgatcgcga atttccacca ggtcgtacac tgggccaaaa
    18061 acgttttcga gcgtggcgag cgcgaaggtg accaccatct gtgtgccgat ataagaagag
    18121 cgttcagagc tggtggcaat ccatgcgccc aacaggaaaa tcggtgccag cacaaacagc
    18181 aattcgacaa tattgtccag ccagggcatg accagtagcg tgaataacag cgccagaatc
    18241 gcgccgcaaa aggccccgcc aaaacgcagc accatcttct ggtacgacga accgacattt
    18301 ggattagcga cgatcacgca ggtcagcata caggtgtgaa tgccttccca gtccacgccg
    18361 ctgtaaaagg tgtaacagat caaacaggcg agcaatgttt ttaccgcgta gcgtatatag
    18421 tctggattgg taaaagcatc ggcgaccatt gatggcggtt tggcggcggg cgttggcggc
    18481 gtattcgggt tcatctggcc cagttgtaac aacgtctggc agatattctc caggttacat
    18541 tcccgtgccg ccaccgcttc actttcactg agccgccagt cgctttgcca gcactgacct
    18601 tcagcaacgg catgctgcag cttgttgatt tctgaggcta atttttgccg aaattcaata
    18661 attgcctgag aatcagcaaa agaggtggga tcgtagcgat tcagcgtcga gtaaatgtag
    18721 gttaccgttg ccacgcagct ttgccaccat gcgctttgag ttcgccagtt ggcatcgtcc
    18781 gcgagacaaa agacattcag tttttgcagc gccagcgcct ctctttcaat ccgcgtttcg
    18841 ggtagcggtg cgaggctgtc cgtcaggtgg ctaatggcat catcaagccg atcattaagc
    18901 gcttgatgca tttgcgtaat ggcacgattg ggaaaccaca gcacgccgat taacgtcatc
    18961 agcaaggttg gatagaggcc aacaacgata caccacagcg ttaagcgcac gactacttcc
    19021 ggatagtcga gcatggcggg gaaggtttgc ccgtaaatgg cgacagcgaa aaagaccagt
    19081 cccagccgat gggtgcgcat caaaaacatg cagcccatca ggatcggccc ggcgatgatc
    19141 aatcggatca acggttcgcc gtatgaccat ttgtagatca aaaacaggct gccgatctcc
    19201 agcaccgtgg caaccacaaa caagatcgcc acaaatttgg tgtaaaacgc gttcgactga
    19261 ataccgtaaa acagcactgc cagcgataac gccacaaaag ggatctcaaa agtcatcgag
    19321 atcagaatca ccagcaggca gcctacccag agttgcacga tctgcggcac gcggccgggt
    19381 cgccgctcgc ttaactcttc atgaaagaac gccagcagcc tgaccaccgg taatggcagg
    19441 gagttgagcg cgctcattat tgcggctcaa gattagcgac tgccgaagcg ccgatgcgga
    19501 acatttccgg gtcaggttta tcgaccatga ttttgaccgg aaaacgctgg gcaacgcgga
    19561 cccagttaat agaacgagac acttttggca ggccgcccag caccaggccg ccgtcatccg
    19621 gtagcacgcc gtagccaatc gaatccactt taccctcgaa ggttttgccg ctgtcactca
    19681 tcaggcgaat cgttgcgggt gtacctgagc gaatattttt cagatcggtt tcgcggaagt
    19741 tggcgatcac gtaccagtga cgagtgtcga tgagggtaaa aatagggcgc atggcagaag
    19801 caaattgccc gacggaggtt ttgagggaaa tgatccggcc atcaaatggc gcgcgaacgg
    19861 tcgtcatttc cagatgtagt ttggtcaggg caatatccgc ttcgaccgcc gcacgttgcg
    19921 ccaccagcgc atcgacgccg ctgacggcgc tggcggctga ctgcgcctgt aacaatacgg
    19981 cattaagatc cgcttctgcg gcgcgctgcg ccgttcttgc acggtcaacg tcttctgctg
    20041 agacaaaacc ctctttcagt aatggctcgg tgcggcgtaa tgtatctgtg gcctgtttcg
    20101 cggcggcacg ggctttttct accgtggcat taaccgagtc ggcaccaaac tgttgggcgt
    20161 caacgctacg ctgggtgagc ataatttgct tatccagcgc cgcgagggag gcttcggctt
    20221 tcgccaggtt ggcttcgtac gggcgcgggt cgatgcggaa cagcaaatcg ccctgtttca
    20281 ccgcctggtt gtcggtgacc gccagttcta caatgcgacc gctgacttcc ggcaccacat
    20341 caatggtatc tgctgacgcg taagcgtcat tagttgatgg cgcactgtct acgcgccaga
    20401 taacgaaaac aagggcaacc agcgccaacg caaccactaa cagagcaggg aatttactgc
    20461 gaggtgcttt tttcggcgta ctttccatga gcgcgtctca aatagattag aaaaatgcca
    20521 gccagaacag catggcgtag agagcaaaaa gggcggtata aataattccg acaaatgcca
    20581 gattgatatt tgcccg
//
LOCUS       DAZPAB010000059        20425 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZPAB010000059 DAZPAB010000000
VERSION     DAZPAB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.059"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHUPG_001902"
     CDS             <1..122
                     /locus_tag="ACHUPG_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR0861537.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
     gene            138..686
                     /gene="wcaF"
                     /locus_tag="ACHUPG_001903"
     CDS             138..686
                     /gene="wcaF"
                     /locus_tag="ACHUPG_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR0861538.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            712..1833
                     /gene="gmd"
                     /locus_tag="ACHUPG_001904"
     CDS             712..1833
                     /gene="gmd"
                     /locus_tag="ACHUPG_001904"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR0861539.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            1836..2804
                     /gene="fcl"
                     /locus_tag="ACHUPG_001905"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             1836..2804
                     /gene="fcl"
                     /locus_tag="ACHUPG_001905"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR0861540.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            2804..3283
                     /gene="gmm"
                     /locus_tag="ACHUPG_001906"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             2804..3283
                     /gene="gmm"
                     /locus_tag="ACHUPG_001906"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR0861541.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            3280..4503
                     /gene="wcaI"
                     /locus_tag="ACHUPG_001907"
     CDS             3280..4503
                     /gene="wcaI"
                     /locus_tag="ACHUPG_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR0861542.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            4506..5942
                     /gene="cpsB"
                     /locus_tag="ACHUPG_001908"
     CDS             4506..5942
                     /gene="cpsB"
                     /locus_tag="ACHUPG_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR0861543.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            6135..7512
                     /gene="cpsG"
                     /locus_tag="ACHUPG_001909"
                     /gene_synonym="manB"
                     /pseudo
     CDS             6135..7512
                     /gene="cpsG"
                     /locus_tag="ACHUPG_001909"
                     /gene_synonym="manB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
     gene            7567..8961
                     /gene="wcaJ"
                     /locus_tag="ACHUPG_001910"
     CDS             7567..8961
                     /gene="wcaJ"
                     /locus_tag="ACHUPG_001910"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFR0861544.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            8963..10441
                     /gene="wzxC"
                     /locus_tag="ACHUPG_001911"
     CDS             8963..10441
                     /gene="wzxC"
                     /locus_tag="ACHUPG_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFR0861545.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            10621..11901
                     /gene="wcaK"
                     /locus_tag="ACHUPG_001912"
     CDS             10621..11901
                     /gene="wcaK"
                     /locus_tag="ACHUPG_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFR0861546.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            11898..13118
                     /gene="wcaL"
                     /locus_tag="ACHUPG_001913"
     CDS             11898..13118
                     /gene="wcaL"
                     /locus_tag="ACHUPG_001913"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFR0861547.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            13129..14523
                     /gene="wcaM"
                     /locus_tag="ACHUPG_001914"
     CDS             13129..14523
                     /gene="wcaM"
                     /locus_tag="ACHUPG_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFR0861548.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            14698..15591
                     /gene="galF"
                     /locus_tag="ACHUPG_001915"
     CDS             14698..15591
                     /gene="galF"
                     /locus_tag="ACHUPG_001915"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFR0861549.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            15964..17049
                     /gene="rfbB"
                     /locus_tag="ACHUPG_001916"
     CDS             15964..17049
                     /gene="rfbB"
                     /locus_tag="ACHUPG_001916"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR0861550.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            17049..17948
                     /gene="rfbD"
                     /locus_tag="ACHUPG_001917"
     CDS             17049..17948
                     /gene="rfbD"
                     /locus_tag="ACHUPG_001917"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFR0861551.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            18007..18885
                     /gene="rfbA"
                     /locus_tag="ACHUPG_001918"
     CDS             18007..18885
                     /gene="rfbA"
                     /locus_tag="ACHUPG_001918"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR0861552.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            18890..19435
                     /gene="rfbC"
                     /locus_tag="ACHUPG_001919"
     CDS             18890..19435
                     /gene="rfbC"
                     /locus_tag="ACHUPG_001919"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFR0861553.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            19451..>20425
                     /gene="wzx"
                     /locus_tag="ACHUPG_001920"
     CDS             19451..>20425
                     /gene="wzx"
                     /locus_tag="ACHUPG_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HFR0861554.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGEN"
BASE COUNT         5123 a         4835 c         5492 g         4975 t
ORIGIN      
        1 gtgctgacgc caaaaaagtc caacggcaga tattacatgt acctggcttc tgggcagaat
       61 tatcctggca tttacgccaa cgtacgacag gtaagactaa agccttatat aacaaagtct
      121 gaatataagg aaaaccaatg caagatttaa gcggtttctc ggtgccgaaa gggttccggg
      181 gcggcaacgc tattaaagtg caattatggt gggcagtaca ggcaacaata tttgcctggt
      241 cgccacaagt attgtatcgc tggcgggctt ttttattacg tttattcgga gcaaaaatag
      301 gaaaaaacgt agttattcgt ccgtcagtaa aaattaccta tccgtggaaa ttaaccttag
      361 gtgattacgc gtgggttggc gatgacgtca atttatatac cctcggtgaa ataaccattg
      421 gcgcacattc ggtgatatcg caaaaaagtt atttatgcac cggtagccac gaccatgcaa
      481 gtcaacattt caccattaac gccacgccta ttgtgattgg cgagaaatgc tggctggcaa
      541 ccgatgtctt tgttgcccca ggcgtcacaa tcggcgacgg caccgtcgtg ggtgcacgaa
      601 gcagtgtttt taaatcgctt ccggcaaatg tggtttgccg ggggaatccc gcagtggtga
      661 tacgcgaacg cgttgaaact gaataaattc aaaaatacag aggaataaga catgtcaaaa
      721 gtcgctctca tcaccggtgt aaccggacaa gacggttctt acctggcaga gtttctgctg
      781 gaaaaaggtt acgaggtgca tggtattaag cgtcgcgcat cgtcattcaa caccgagcgc
      841 gtggatcaca tttatcagga tccgcacacc tgcaacccga aattccatct gcattatggc
      901 gacctgagtg atacctctaa cctgacgcgc attttgcgtg aagtacagcc ggatgaagtg
      961 tacaacctag gcgcaatgag ccacgttgcg gtctcttttg agtcaccaga atataccgct
     1021 gacgtcgacg cgatgggtac gctgcgcctg ctggaggcga tccgcttcct cggtctggaa
     1081 aagaaaactc gtttctatca ggcttccacc tctgaactgt atggtctggt gcaggaaatt
     1141 ccgcagaaag aaaccacgcc gttctacccg cgatctccgt atgcggtcgc caaactgtac
     1201 gcctactgga tcaccgttaa ctaccgcgaa tcctacggca tgtacgcttg taacggaatt
     1261 ctcttcaacc atgaatcccc gcgccgcggc gaaaccttcg ttacccgcaa aatcacccgc
     1321 gcaatcgcca acatcgccca ggggctggag tcgtgcctgt acctcggcaa tatggattcc
     1381 ctgcgtgact ggggccacgc caaagactac gtaaaaatgc agtggatgat gctgcaacag
     1441 gaacagccgg aagatttcgt tatcgcgacc ggcgttcagt actccgtacg tcagtttgtg
     1501 gaaatggcgg cagcacagct gggcatcaaa ctgcgctttg aaggcacggg tgttgaagag
     1561 aagggcattg tggtttccgt caccgggcat gacgcgccgg gcgttaaacc gggtgatgtg
     1621 attatcgccg ttgatccgcg ttacttccgt ccggcagaag ttgaaacgct gctcggcgac
     1681 ccgaccaaag cgcacgaaaa actgggctgg aaaccggaaa tcaccctcag agagatggtg
     1741 tctgaaatgg tggctaatga cctcgaagcg gcgaaaaaac actctctgct gaaatctcac
     1801 ggctacgacg tggcgatcgc gctggagtca taagcatgag taaacaacgc atttttatcg
     1861 ctggccattg tgggatggtc ggttctgcca tcaagcggca gctcgaacag cgcggtgatg
     1921 tggagctggt attacgtacc cgcgatgaac tgaacctgct ggacagccgc gccgtgcatg
     1981 atttctttgc cagcgaacgc attgaccagg tctatctggc ggcggcgaaa gtgggcggca
     2041 ttgttgccaa caacacctat ccggcggatt tcatctacca gaacatgatg attgagagca
     2101 acatcattca cgccgcgcat cagaacgacg tgaacaaact gatgtttctc ggatcgtcct
     2161 gcatctaccc gaaactggca aaacagccga tggcagaaag cgagttattg cagggcacgc
     2221 tggagccgac caacgagcct tacgccattg ccaaaatcgc cgggatcaaa ctgtgcgaat
     2281 cttacaaccg ccagtatgga cgcgattacc gcactgtcat gccgaccaac ctgtacgggc
     2341 cgcatgacaa cttccacccg agtaactccc atgtgatacc agcattgctg cgtcgcttcc
     2401 acgaggcgac ggcacagaat gcgccggacg tggtggtatg gggcagcggt acaccgatgc
     2461 gtgaattcct gcacgtcgat gatatggcgg cggcgagcat tcatgtcatg gagctggcgc
     2521 atgaagtctg gctggagaat acccaaccga tgctgtcgca cattaacgtc ggcacgggcg
     2581 ttgactgcac tatccgcgag ctggcgcaaa ccatcgccaa agtggtgggt tacaaaggtc
     2641 gggtggtttt tgatgccagc aaaccggatg gtacgccgcg caaactgctg gatgtgacgc
     2701 gcctgcatca gcttggctgg tatcacgaaa tctcactgga agcggggctt gccagcactt
     2761 accagtggtt ccttgagaat caagaccgct ttcgggggga atgatgtttt tacgtcagga
     2821 agactttgcc actgtagtgc gctccacacc gcttgtctct ctcgacttta ttgtcgagaa
     2881 cagtcgcggc gagtttctgc ttggcaaaag aaccaaccgc ccggcgcagg gttactggtt
     2941 tgtgccggga gggcgcgtgc agaaagacga aacgctggaa gccgcatttg agcggctgac
     3001 gatggcggaa ctggggctgc gtctgccgat aacagcaggc cagttttacg gtgtctggca
     3061 gcatttttat gacgataact tctccggcac ggatttcacc actcactatg tggtgctcgg
     3121 ttttcgcttc agagtagcgg aagaagagct gttactgccg gatgagcagc atgacgatta
     3181 ccgctggctg acgccggacg cgctgctcgc cagtaacgat gttcacgcta acagccgagc
     3241 ctattttctc gctgagaagc gtgccggagt acccggatta tgaaaatact ggtctacggc
     3301 attaactact cgccggagtt aaccggcatc ggcaaataca ccggcgagat ggtggaatgg
     3361 ttggcggcac aaggtcatga ggtgcgggtc attaccgcac cgccttacta cccgcaatgg
     3421 caggtgggtg agaactattc cgcctggcgc tacaaacgag aagaaggggc cgccacggtg
     3481 tggcgctgcc cgctgtacgt gccaaaacag ccgagcaccc tgaaacgctt gttgcatctc
     3541 ggcagttttg ccgtcagcag tttctttcca ctgatggcgc aacgtcgctg gaagccggat
     3601 cgcattattg gcgtagtgcc aacgctgttt tgcacgccgg gaatgcgcct gctggcgaag
     3661 ctctctggtg cgcgtaccgt gctgcatatt caggattacg aagtggacgc catgctgggg
     3721 ctgggccttg ccggaaaagg caaaggcggc aaagtggcac agctggcaac ggcgttcgaa
     3781 cgtagcggac tgcataacgt cgataacgtt tccacgattt cgcgttcgat gatgaataaa
     3841 gccatcgaaa aaggcgtggc ggcggataac gtcatcttct tccccaactg gtcggaaatt
     3901 gcccgttttc agcatgttgc agacgccgat gttgatgccc ttcgtaacca gcttggcctg
     3961 ccggataaca aaaaaatcat tctttactcc ggcaatattg gtgaaaagca ggggctggaa
     4021 aacgttattg aagctgccga tctcctgcgc gatgaaccgc tgatttttgc cattgtcggg
     4081 cagggcggcg gcaaagcgcg gctggaaaaa atggcgcagc agcgtggact gcgcaacatg
     4141 caatttttcc cgctgcaatc gtatgacgct ttacctgcac tgctgaagat gggcgattgc
     4201 catctggtgg tgcaaaaacg cggcgcggca gatgccgtat tgccgtcgaa actgaccaat
     4261 attctggcgg taggcggtaa cgcggtgatt accgcagagg cccacacaga actggggcag
     4321 ctttgcgaaa cctttccggg tattgcggtt tgcgtagaac cggaatcggt cgaggcgctg
     4381 gtggcgggga ttcgtcaggc gctcctgctg cccaaacata acacggtggc acgtgaatat
     4441 gccgaacgca cgctcgataa agagaacgtg ttacgtcaat ttataaatga tattcgggga
     4501 taattatggc gcagtcgaaa ctctatccag ttgtgatggc aggtggctcc ggtagccgtt
     4561 tatggccgct ttcccgcgta ctttacccca agcagttttt atgcctgaaa ggcgatctca
     4621 ccatgctgca aaccaccatc tgccgcctga acggcgtgga gtgcgaaagc ccggtggtga
     4681 tttgcaatga gcagcaccgc tttattgtcg cggaacagct gcgtcaactg aacaaactca
     4741 ccgagaacat tattctcgaa ccggcagggc gtaacactgc acctgccatt gcactggcgg
     4801 cgctggcggc aaaacgtcat agcccggaga gcgacccgtt aatgctggta ttggcggcgg
     4861 atcatgtgat tgccgatgaa gacgcgttcc gtgccgccgt gcgtaatgcc atgccttatg
     4921 ccgaagcggg caagctggtg acctttggca ttgtgccgga tctaccagaa accggttatg
     4981 gctatattcg tcgcggtgaa gtgtctgtgg gtgagcagga tacggtggcc tttgaagtgg
     5041 cgcagtttgt cgaaaaaccg aatctggaaa ccgcccaggc ctatgtagca agcggcgaat
     5101 attactggaa cagcggcatg ttcctgttcc gcgccggacg ctatctcgaa gaactgaaaa
     5161 aatatcgccc ggatatcctc gatgcctgcg aaaaagcgat gagcgccgtc gatccggatc
     5221 tcgattttat tcgtgtggat gaagaagcgt ttctcgcctg cccggaagag tcggtggatt
     5281 acgcggtcat ggaacctacg gcagatgctg ttgtggtgcc gatggatgcg ggctggagcg
     5341 atgttggttc ctggtcttca ttatgggaga tcagcgccca caccgccgag ggcaacgttt
     5401 gccacggcga tgtgattaat cacaaaactg aaaacagcta tgtgtatgcc gaatcaggcc
     5461 tggtcaccac cgtcggggtg aaagatctgg tagtggtgca gaccaaagat gcggtgctga
     5521 ttgccgaccg taacgcggta caggatgtga aaaaagtggt cgagcagatc aaagccgatg
     5581 gtcgccatga gcatcgggtg catcgcgaag tgtatcgtcc gtggggcaaa tatgactcta
     5641 tcgacgcggg cgaccgctac caggtgaaac gcatcaccgt gaaaccgggc gagggtttgt
     5701 cggtacagat gcaccatcac cgcgcggaac actgggtagt ggtcgcggga acggcaaaag
     5761 tcaccattga tggtgatatc aaactgcttg gtgaaaacga gtccatttat attccgctgg
     5821 gggcgacgca ctgcctggaa aacccgggga aaattccgct cgatttaatt gaagtgcgct
     5881 ccggctctta tctcgaagag gatgatgtgg tgcgcttcgc ggatcgctac ggacgggtgt
     5941 aaacgtcgca tcaggcgctg tttgtcggat gcggcgtgaa cgccttatcc gacctacggt
     6001 tcggttttgt aggcctgata agacgcggca gcgtcgcatc aggcaatgaa tgcgaaaccg
     6061 cggtgtaaat aacgacaaaa ataaaattgg ccgtttcggt cagggccaac tattgcctga
     6121 aaaagggtaa cgacatgaaa aaattaacct gctttaaagc ctatgatatt cgcgggaaat
     6181 taggcgaaga actgaatgaa gatatcgcct ggcgcattgg tcgcgcctat ggcgaatttc
     6241 tcaaaccgaa aaccattgtg ttaggcggtg atgtccgtct caccagcgaa accttaaaac
     6301 tggcgctggc aaaaggttta caggatgcgg gcgtcgatgt gctggatatc ggtatgtccg
     6361 gcaccgaaga gatctatttc gccacgttcc atctcggcgt ggatggcggc attgaagtta
     6421 ctgccagcca taatccgatg gattacaacg gcatgaagct ggtgcgcgag ggggctcgca
     6481 cgatcagcgg tgataccgga ctgcgcgacg tccagcgtct ggctgaagcc aacgactttc
     6541 ctcccgtcga tgaaaccaaa cgcggtcgct atcagcaaat caacctgcgt gacgcttacg
     6601 ttgatcacct gttcggttat atcaatgtca aaaatctcac gccgctcaag ctggtgatca
     6661 actccgggaa tggcgcagcg ggtccggtgg tggacgccat tgaagcccgc tttaaagccc
     6721 tcggcgcgcc cgtggaatta atcaaagtac acaacacacc ggacggcaat ttccccaacg
     6781 gtattcctaa cccgctgctg ccggaatgtc gcgacgacac ccgcaatgcg gtcatcaaac
     6841 acggcgcgga tatgggcatt gcctttgatg gcgattttga ccgctgtttt ctgtttgacg
     6901 aaaaagggca gtttatcgag ggctactaca ttgtcggcct gctggcagaa gcgttcctcg
     6961 aaaaaaatcc cggcgcgaag atcatccatg atccgcgatc cgcgtctctc ttggaacact
     7021 attgatgtgg tgactgctgc aggcggcacg ccggtaatgt cgaaaaccgg acacgccttt
     7081 attaaagaac ggatgcgcaa ggaagacgcc atctacggtg gcgaaatgag cgcccaccat
     7141 tacttccgtg atttcgctta ctgcgacagc ggcatgatcc cgtggctgct ggtcgccgaa
     7201 ctggtgtgcc tgaaaggaaa aacgctgggc gaactggtgc gcgaccggat ggcggcgttt
     7261 ccggcaagcg gtgagatcaa cagcaaactg gcgcaacccg ttgaggcgat taaccgcgtg
     7321 gaacagcatt ttagccgtga ggcgctggcg gtggatcgca gcgatggcat cagcatgacc
     7381 tttgccgact ggcgctttaa cctgcgcacc tccaataccg aaccggtggt gcgcctgaat
     7441 gtggaatcgc gcggtgatgt gccgctgatg gaagcgcgaa cgcgaactct gctgacgttg
     7501 ctgaacgagt aatgtcggat cttcccttac cccactgcgg gtaaggggct aataacagga
     7561 acaacgatga caaatctaaa aaagcgcgag cgagcgaaaa ccaatgcatc gttaatctct
     7621 atggtgcaac gcttttcaga tatcaccatc atgtttgccg ggctatggct ggtttgcgaa
     7681 gtcagcggac tgtcattcct ctacatgcac ctgttggtgg cgctgattac gctggtggtg
     7741 ttccagatgc tgggcggcat caccgatttt tatcgctcat ggcgcggtgt tcgggcagcg
     7801 acagaatttg ccctgttgct acaaaactgg accttaagcg tgattttcag cgccggactg
     7861 gtggcgttca acaatgattt cgacacgcaa ctgaaaatct ggctggcgtg gtatgggctg
     7921 accagcatcg gactggtggt ttgccgttca tgtattcgca ttggggcggg ctggctgcgt
     7981 aatcatggct acaacaagcg gatggtcgcc gtagcggggg atttagccgc cggacaaatg
     8041 ctgatggaga gtttccgtaa tcagccgtgg ttagggtttg aagtggtggg cgtttaccac
     8101 gacccgaaac cgggcggcgt ttctaacgac tgggcgggta acctgcaaca gctggtcgag
     8161 gatgcaaaag cgggcaagat tcataacgtc tatatcgcga tgcaaatgtg cgacggcgca
     8221 cgagtgaaaa aactggtcca tcaactggcg gataccacct gttcggtgct gctgatcccc
     8281 gacgtcttta ccttcaacat tctccattca cgtctcgaag agatgaacgg cgttccggtg
     8341 gtgccgctgt atgacacgcc gctttcgggg gttaaccgcc tgctaaaacg tgcggaagac
     8401 attgtgctgg cgacgcttat tctgctgctg atctccccgg tgctgtgctg tattgcgctg
     8461 gcggtgaaac tcagttcacc agggccggtt attttccgcc agactcgcta tggtatggat
     8521 ggcaagccga tcaaagtgtg gaagttccgt tccatgaaag tgatggagaa cgacaaagtg
     8581 gtgacccagg cgacgcagaa cgatccgcgc gtcactaaag tggggaactt tctgcgccgc
     8641 acctcgctgg acgaattgcc gcagtttatc aatgtgctga ccggggggat gtcgattgtc
     8701 ggtccacgtc cgcacgcagt agcgcataac gaacagtatc gccagctcat tgaaggctac
     8761 atgctgcgcc ataaagtgaa accgggcatt accggctggg cgcagattaa tggctggcgc
     8821 ggcgaaaccg acacgctgga gaaaatggaa aaacgcgtcg agttcgacct tgagtacatc
     8881 cgcgaatgga gcgtctggtt cgatatcaaa atcgttttcc tgacggtatt caagggcttc
     8941 gttaacaaag cggcatattg atatgagctt acgtgaaaaa accatcagcg gcgcgaagtg
     9001 gtcggctatt gccacggtga tcatcatcgg cctcgggctg gtgcagatga ccgtgctggc
     9061 gcggattatc gacaaccacc agttcggcct gctgactgtg tcgctggtga ttatcgcgct
     9121 ggcagatacg ctttctgact tcggtatcgc taactcgatt attcagcgaa aagaaatcag
     9181 tcaccttgaa ctcaccacgc tgtactggct gaacgtcggg ctggggcttg tggtatgtgt
     9241 ggcggtgttt ttgttgagtg atgtcatcgg cgacgtgctg aataacccgg acctggcacc
     9301 gttgattaaa acattgtcgc tggcgtttgt ggtgatcccc cacgggcaac agttccgcgc
     9361 gttgatgcag aaggagctgg agttcaacaa aatcggcatg atcgaaacca gcgcggtgct
     9421 ggcgggcttc acttttacgg tagttagcgc ccatttctgg ccgctggcga tgaccgcgat
     9481 cctcggttat ctggtcaata gtgcggtgag aacactgctg tttggctact ttggacgcaa
     9541 aatttattgc cccggtctgc acttctcgct ggcgtctgtg gcaccgaact tacgttttgg
     9601 tgcctggctg acggcggaca gcatcatcaa ctatctcaac accaaccttt caacgctggt
     9661 gctggcgcgt attcttggcg cgggcgtggc agggggatac aacctggcgt acaacgtggc
     9721 cgtcgtaccg ccgatgaagc tgaacccaat catcacccgc gtgttgtttc cggcgtttgc
     9781 caaaattcag gacgacaccg aaaagctgcg tgttaacttc tacaagctgc tgtcgatagt
     9841 ggggattatc aactttccgg cgctgctggg gctgatggtg gtgtcgaata acttcgtacc
     9901 gctggtattc ggcgaaaagt ggaacagcat tattccggtg ctgcaattgc tgtgtattgt
     9961 gggtctgctg cgctccgtgg gtaacccgat tggttcgctg ctgatggcga aagcgcgggt
    10021 cgatatcagc tttaaattca acgtattcaa aacatttctg tttattccgg cgattgttat
    10081 tggtgggcag atggcgggcg caatcggcgt cacgctcggt ttcctgctgg tgcagattat
    10141 caacaccatt ctgagttact tcgtgatgat taaaccggtt cttggttcca gttatcgcca
    10201 gtacatcctg agtttgtggc tgccgtttta tctctcgttg ccgacgctgg tggtcagtta
    10261 tgcgctgggc attgtgctga aagggcaact ggcgctgggg atgctgctgg cggtgcaaat
    10321 agccgcgggc gtgctggcgt ttgtggtgat gattgtgctg tcacgccatc cgctggtggt
    10381 ggaagtgaag cgtcagtttt gtcgcagcga aaaaatgaaa atgcttttac gggcggggtg
    10441 aattgctgtt ttcggttccg acaacggttg ccggatgcgg cgtaaaacgc cttatccgtc
    10501 ctacggttct gtgctcggga cctgtaggcc tgataagacg cgccagcgtc gcatcaggca
    10561 aaaaacggca acgaacacaa caaacacagc ctcacctggc atcaaattta tgaggaaaaa
    10621 atgaaattac ttattctggg caaccacact tgcggcaatc gtggcgacag cgccatcttg
    10681 cgcggcttac ttgatgccat caacatcctc aatccacata ccgaagtgga cgtgatgagc
    10741 cgctatccgg tcagttcttc ctggctgctc aaccgcccgg taatgggcga tccgctgttc
    10801 ctgcagatga cacaacacaa tagcgcggcg ggcgttgtcg ggcgcattaa aaaagtcctc
    10861 cgtcgccgct accagcatca ggtattgctc tcacgcgtca ccgacactgg caagctgcgc
    10921 aatatcgcca tcgcccaggg attcaccgac ttcgtgcgcc tgctgtcagg ttacgacgcc
    10981 attattcagg tcggcggatc gttttttgtc gatctctacg gcgtgccgca gtttgaacat
    11041 gcgctttgca catttatggc gaaaaagccg ctgtttatga ttggtcacag cgtcggcccc
    11101 ttccaggatg agcaatttaa ccaactggcg aactatgtct ttggtcactg cgacgcgctg
    11161 atcctgcgcg aatcggtcag ccttgatttg atgaaacgca gaaatatcac caccgcaaaa
    11221 gtggaacatg gcgtcgatac cgcgtggctg gtcgatcacc acaccgaaga cttcaccgcc
    11281 agctatgccg ttcaacactg gctggacgtt gccgcacaac agaaaacagt ggcaattacc
    11341 ctgcgcgaac tggcaccgtt cgacaaacgt ctcggcacca ctcaacaagc gtatgaaaaa
    11401 gcctttgccg gggtggtcaa tcgcattctc gatgaaggct atcaggtgat tgcgctctcc
    11461 acctgtacgg gtatcgacag ctataacaaa gacgaccgca tggtggcgct caatctgcgc
    11521 cagcacatca gcgatcctgc ccgttaccac gtagtgatgg atgaactcaa cgatctggaa
    11581 atgggcaaaa ttctcggagc ctgtgaactc accgtcggta cgcgcctgca ctccgccatt
    11641 atctcaatga actttgctac tccggcgatt gccatcaact atgaacataa atccgccggg
    11701 attatgcagc agctgggact gccagagatg gcgattgata ttcgtcattt actggacggc
    11761 agcctgcaag cgatggttgc ggatacctta ggccagcttc cggtgctgaa cgctcgattt
    11821 aacgaagccg tcagtcgcga acgtcagacg ggaatgcaga tggtgcagtc tgtgcttgag
    11881 cgcatcgggg aggtgaaatg aaggtcggct tcttcttatt gaaattcccg ctgtcgtcgg
    11941 aaaccttcgt cctcaatcaa attaccgcgt ttattgatat gggctttgaa gtggagattg
    12001 tcgctttgca aaaaggcgat acagagaaca cccacgcggc atggacgaaa tacaaccttg
    12061 tcgccagaac ccgctggtta caggacgaac ctgcgggcaa agtagcgaaa ctgcgccacc
    12121 gcgccagcca gaccttacgt ggcattcatc gtaaaaatac ctggcatgcg ctcaacctca
    12181 aacgctatgg tgccgagtcg cggaacctga ttttgtctgc catttgtggt caggtcgcaa
    12241 caccgtttcg cgccgatgtg ttcatcgctc attttggccc ggcgggggta accgcagcaa
    12301 aactacgcga actgggtatc attcgcggca aaattgccac catcttccac ggtattgata
    12361 tctccagtcg ggaagtgctc aaccactaca ctcccgaata tcaacaactg tttcgccgtg
    12421 gcgacctgat gttaccgata agcgatctgt gggccggaag gctgcaaaaa atgggctgcc
    12481 ccagagaaaa aatcgccgta tcgcgtatgg gcgtggacat gacgcgcttt agcccgcgtc
    12541 cggtgaaagc gcccgcaacg ccgctggaaa tcatatctgt cgcacgctta accgagaaaa
    12601 aaggcctgca tgtggcgatt gaagcctgcc gtcagttgaa agagcagggc gtggcatttc
    12661 gctatcgcat cctcggcatt ggcctgtggg aacgacgcct gcgcaccatc atcgaacaat
    12721 atcaactgga agatgtggta gagatgccgg gctttaaacc gagccatgaa gtgaaggaga
    12781 tgctcgacga cgcggatgtc ttcctgttgc catcggtaac gggcgcggat ggcgatatgg
    12841 aaggcattcc ggtggcgcta atggaagcga tggcggtcgg tattccggtg gtttctactc
    12901 tgcatagcgg aattcctgaa ctggtggagg ccgataaatc cggctggctg ctgcctgaga
    12961 acgatgctcg cgcactggca caacgtctgg cggcgtttag ccaactggat accgacgaac
    13021 tggctccggt cgtcaaacgt gcgtgcgaaa aagtcgaaca cgattttaac cagcaggtga
    13081 ttaatcgaga actcgccagc ttgctgcagg ctttatagag gagaacgtat gccatttaaa
    13141 acactctccc gacgcacctt cctgacggca agctcggcgc ttgccttcct ccatacccct
    13201 ttcgctcgcg cacttcccgc ccgacaaagc gttaacatta acgactacaa cccacatgac
    13261 tggatcgcct catttaaaca agccttcagc gaagggcaaa cggtcgtcgt gcctgccgga
    13321 ttcgtttgtg acaatatcaa caccggcatc ttcatccctc ccggcaaaac gttacacatc
    13381 cttggaagcc tgcgcggcaa cggcagaggg cgatttgtct tacaggacgg cagccaggtg
    13441 acagggggag agggcggcgg tatgcataac attaccctgg atgtgcgtgg ttctgactgc
    13501 accatcaaag ggctggcgat gagcggcttt ggcccggtaa tgcagattta tattggcggc
    13561 aaaaacaaac gggtcatgcg caacctgacc atcgataacc tcactgtcag ccacgctaat
    13621 tacgccatct tacgccaggg atttcataac cagattatcg gtgccaacat taccaactgt
    13681 aagttcagcg acttacaggg cgacgccatt gaatggaacg tggcaattaa cgacagtgat
    13741 attttgatat ctgaccatgt catcgagcgt atcaactgta ccaacggcaa aatcaactgg
    13801 ggcatcggca taggccttgc gggaagcact tatgataaca actacccgga agaccaggca
    13861 gtgaaaaact ttgtcgtggc gaatatcacg ggatcggatt gtcggcagtt gatccatgtt
    13921 gaaaatggta aacattttgt tattcgtaat atcaatgccc gcaatatcac gccggatttc
    13981 agtaagaaag cgggcattga taacgcgaca gtcgctattt acggttgtga caatttcgtg
    14041 attgataata ttgaaatgat taatagcgcc gggatgttaa tcggctatgg ggtaattaaa
    14101 ggcaaatatc tctcgatacc gcaaaatttc cgagtgaata atattcaact ggataatacc
    14161 catcttgctt ataaattgcg cggcatccaa atctccgccg ggaatgccgt ctcctttgtg
    14221 tcgctaacta acattgagat gaagcgtgcc tcgctggagt tacacaacaa gccgcaacat
    14281 ctttttatgc gaaatatcaa tgtgatgcag gaatcctcag ttggaccagc attgagcatg
    14341 aacttcgaca tgcgcaaaga cgttcgcggc gtctttatgg cgaaaaaaga aacactgctg
    14401 tctcttgcaa atgttcatgc ggtgaatgaa agagggcaaa gctccgtcga tatcgacagg
    14461 attaatcacc atattgttaa tgtggaaaag attaacttta gattgccgga acggagggag
    14521 tagatttgcg accattcctg gaaaaatgga gccatactta ggaacaatgc tactgcaatc
    14581 cacaacgaag cggcgtaaca tcacaagtaa ttcagtaatc aattcagggt aattgatgct
    14641 ggcgaaaaaa atcgaacaag ctataattca gcaaccattt tacaggtgga tgaaataatg
    14701 acgaatttaa aagcagttat tcctgtagcg ggtctcggga tgcatatgtt gcctgccact
    14761 aaggcgatac ccaaagagat gctaccaatc gtcgacaagc caatgattca gtacattgtt
    14821 gacgagattg tggctgcagg gatcaaagaa atcctcctgg taactcacgc gtccaagaac
    14881 gcggtcgaaa accacttcga cacctcttat gagttagaat cactccttga gcagcgcgtg
    14941 aagcgtcaac tgctggcgga agtacagtcc atctgtccgc cgggcgtgat cattatgaac
    15001 gtgcgtcagg gcgaaccttt aggtttaggc cactccattt tgtgtgcgcg acctgccatt
    15061 ggtgacaacc catttgtcgt ggtactgcca gacgttgtga tcgacgatgc cagcgccgac
    15121 ccgctacgtt acaaccttgc tgccatgatt gcacgtttca acgaaacggg ccgcagccag
    15181 gtgctggcaa aacgtatgcc gggtgacctc tctgaatact ccgtcatcca gactaaagag
    15241 ccgctggacc gtgagggtaa agtcagccgc attgttgaat ttatcgaaaa accggatcag
    15301 ccgcagacgc tggactcaga catcatggcc gtaggtcgct atgtgctttc tgccgatatt
    15361 tggccggaac tggaacgtac tcagcctggt gcatggggac gtattcagct gactgatgct
    15421 attgccgagc tggcgaaaaa acaatccgtt gatgcaatgc tgatgaccgg cgacagttac
    15481 gactgcggca aaaaaatggg ctatatgcag gcgtttgtga agtatggcct acgcaacctg
    15541 aaagaagggg cgaagttccg taaaggtatt gagaagctgt taagcgaata atgaaaatct
    15601 gaccggatgt aacggttgat aagaaaatta taacggcagt gaagattcgt ggcataagtt
    15661 ttgctcattg aattttcctg ccgttgccgt ttttataaag cctataaaaa caagaagtta
    15721 catagctcat attattcaaa tttttccagg attttccttg tttctacggc tgtttggtaa
    15781 gacaattaac gtttgaattt ttcggatttg gcgcgagtgg ctaacgctcg ccacatcgtt
    15841 gagatgcatg cagtgcactg gtagctgtta agccaggggc ggtagcgtgt attaatacct
    15901 ctattaatca aatctggagc agtctatttc acagcatgct ctatagctat atggaataaa
    15961 aaagtgaaga tacttgttac tggtggcgca gggtttattg gttctgctgt tgttcgtcac
    16021 ataataaata atacgcaaga tagtgttgtt aatgtcgata aattaacata cgccgggaac
    16081 ctggaatcac tcgctgatgt ttctgattct gaacgttatg cttttgagca tgcagatatc
    16141 tgcgatgctg tagccatgtc gcgtattttc gcacagcacc agccagacgc ggtgatgcac
    16201 ctggcggcag agagccacgt tgaccgttca ataacgggcc ctgcggcatt tattgaaacc
    16261 aatattgtgg ggacttatgt tcttttagaa gcggcgcgca attactggtc tgctctgaat
    16321 gatgaaaaga aaaaaagttt ccgctttcat catatttcta ctgatgaagt ttatggtgac
    16381 ttaccccatc cggatgaagc aaataataac gaagcgttac cgctatttac ggaaacgaca
    16441 gcttacgcgc caagtagccc gtattctgcg tctaaagctt ccagcgatca tttggtacgt
    16501 gcctggaagc ggacttatgg tttaccaacc attgtgacta attgctcgaa caattatggt
    16561 ccttatcact tcccggaaaa acttattcca ttggttattc tgaatgctct ggaaggtaag
    16621 gcattaccta tttatggtaa aggggatcaa attcgcgact ggttgtatgt agaggatcat
    16681 gctcgtgcgt tatataccgt cgtaaccgaa ggtaaagcgg gtgaaactta taacattggt
    16741 ggacataacg aaaagaaaaa catcgacgta gtgctcacta tttgtgattt gttggatgag
    16801 attgtaccga aagagaaatc ttatcgtgag caaattactt atgttgccga tcgcccgggg
    16861 catgaccgcc gttacgccat tgatgccgat aaaattagcc gcgaattggg ctggaaaccg
    16921 caagaaacgt ttgagagcgg gattcgtaaa acggtggaat ggtatctggc taatacaaat
    16981 tgggttgaga atgtgaaaag cggtacttat cagtcgtgga ttgaacaaaa ctatgagggc
    17041 cgtcagtaat gaatatcctg cttttcggca aaacagggca ggtgggttgg gaactgcagc
    17101 gtgctctggc tcctctgggt aacttgattg cgctcgatgt tcactccact gattattgtg
    17161 gagatttcag caaccccgaa ggtgtggctg aaaccgtcaa aaaaattcgc cccgatgtta
    17221 ttgttaatgc tgctgctcac actgcagtag acaaagcgga atcagaaccg aatttcgcgc
    17281 aattacttaa cgcgacatgc gtcgaagcga ttgcaaaagc agctaatgaa gtcggagcct
    17341 gggttataca ctactccact gattatgttt tcccaggcaa cggtgacacg ccatggctgg
    17401 aaacggatgc aacagcaccg ctaaatgtct acggtgaaac caagctagct ggggaaaaag
    17461 cgttacaaga acattgcgca aagcatctta ttttccgtac cagctgggta tacgctggta
    17521 aaggaaataa ctttgccaaa acgatgttgc gcctggcaaa agaacgcgaa gagttggctg
    17581 taataaacga tcaattcggc gcaccaacgg gggctgaatt actggctgat tgcaccgctc
    17641 atgccattcg cgtggccgca aataaaccag aagttgctgg tttgtaccat ctggttgctg
    17701 gtggtacaac aacctggcac gattatgccg ctctggtatt tgaagaagcg cgcagagcgg
    17761 ggatcaacct tgctcttaac aaacttaatg ccgtgccgac aacggcctat cccacaccag
    17821 cacgtcgtcc ccataattct cgcctcaata ccgaaaaatt tcagcagaac tttgcgcttg
    17881 tcttgcctga ctggcaggtg ggcgtgaaac gtatgctcaa cgaattattt acgaccacgg
    17941 caatttaaca aattattgca tctcgctcat gatgctagag cgagatgaat taaaaggaat
    18001 ggtgaaatga aaacgcgtaa aggtattatt ctggctggtg gttccggcac tcgtctttat
    18061 cctgtgacaa tggctgtgag caaacaactg ctaccgattt atgataaacc gatgatttat
    18121 tatccgcttt caacgcttat gttggcgggt attcgagata ttctgattat cagtacgccg
    18181 caggatacac cacgttttca acaattgctt ggtgatggaa gccagtgggg gcttaatctg
    18241 caatataaag tacaaccgag tccggatggt ctggcgcaag catttatcat tggtgaagaa
    18301 tttataggtg gtgatgattg tgcgctggta cttggtgata atatcttcta tggtcacgac
    18361 ctgccgaaat taatggatac cgctgttaac agagagagtg gcgcaacggt ttttgcctat
    18421 cacgtaaatg atcctgagcg ctacggtgtg gtggaatttg atgataacgg tacggcaatt
    18481 agtctggaag aaaaaccgca ggaaccaaaa agtaactatg cggttactgg gctttatttc
    18541 tatgacaatg acgttgtaga aatggcgaaa aaccttaaac catctgcacg tggcgaactg
    18601 gaaattaccg atattaaccg tatttatatg gaccaaggac gtttgtctgt cgctatgatg
    18661 gggcgtggtt atgcatggct ggacacgggg acacatcaaa gtcttattga agcaagcaac
    18721 ttcattgcaa caattgaaga gcgtcagggg ctgaaagttt cctgcccgga agaaattgct
    18781 caccgtaaag gctttattga tgctgagcaa gtgaaggtat tagccgagcc gctgaagaaa
    18841 aatgcttatg gtcagtatct gctgaaaatg attaaaggtt attaataaaa tgaacgtaat
    18901 taaaactgaa attcctgatg tgctaatttt tgaaccaaaa gtatttggtg atgagcgggg
    18961 cttttttatg gaaagcttca atcagaaagt tttcgaagag gctgtaggac gaaaggttga
    19021 gtttgttcag gataaccatt ctaaatcaac taagggtgtg ttacgcggac tgcactatca
    19081 gctggaacct tatgctcaag gaaaactggt acgctgtgtt gtcggtgaag tttttgacgt
    19141 agctgttgat attcggaaat catcaccaac atttggcaag tgggttggtg taaatttgtc
    19201 ggcggagaat aagcgtcaat tatggatacc ggaaggattc gctcatgggt tttgcgttct
    19261 tagtgatgaa gcagaatttg tctacaaaac aaataatttc tattcaaaaa tgcaagagcg
    19321 tgggatttta tggagcgaca aaagtataaa tatagaatgg ccggttcaaa atccattgct
    19381 ttctgataaa gatattaatg gtcaaaaatt tgtagatgct gattatttta tatgataaag
    19441 aaatgtaata atgagcataa taaaaaatag tgtctggaac ctttttggct atgcaatacc
    19501 aactttaatt gctatcccct cgctaggatt tctcgctcga ggtttggggc ctgaaggttt
    19561 cggtgtttat acaattgcaa ttgcacttgt tgggtatgct ggaatttttg atgtaggcct
    19621 gactcgctct gttattagag aaattgcaat tcatcgtgat aatcatcatg aaagaaccaa
    19681 ggtaatttca acaagtacat cttttttggt gctattttca tgctttggtg cttttttatt
    19741 attgattttc tctgatggaa ttgttaatta tctaaaaatt tctggtgttg aacatagtga
    19801 tatacaacta gcatttaaac tgttggctat ttgcattcca ttatttattc taaatcaatt
    19861 atggtcagcc attcttgagg gggatgaaaa atttggcatt gtaaatattc aaaaatctat
    19921 atcaagctct tgcatcgcgg gaattccggc catatttgtt ttttatagtg ctacattgtc
    19981 ggcagcggtt gctggtttaa tatttgcaag agttatttcg attttagtct ctgcctatta
    20041 tgtcaggaat gatattaaaa tttcgggggt tcatttttgt tataaaacct ttaaacgact
    20101 ctttttcttt ggcggttgga tgacagtaag taatatcata agtccggtca tggtgtattt
    20161 tgatcgattt atagtgtcaa atattatggg ggcagataaa gttgcatttt attctgcacc
    20221 agcggaggtt attctaaaat taggaataat acctgcagca atcgggaggg cagtgtttcc
    20281 aaggttaagt aacatcaaag actttaaaga atttaaacgt aatgtaaata aatcattgct
    20341 tttaatgttt ctaatctgtt tgccggtgat aatcataggc ttgttatatt cagggcttgt
    20401 attgaaaata tggtttggtg agaat
//
LOCUS       DAZPAB010000060        20163 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZPAB010000060 DAZPAB010000000
VERSION     DAZPAB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.060"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(40..2109)
                     /gene="glyS"
                     /locus_tag="ACHUPG_001921"
     CDS             complement(40..2109)
                     /gene="glyS"
                     /locus_tag="ACHUPG_001921"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR0861555.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2119..3030)
                     /gene="glyQ"
                     /locus_tag="ACHUPG_001922"
     CDS             complement(2119..3030)
                     /gene="glyQ"
                     /locus_tag="ACHUPG_001922"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR0861556.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3125..3424)
                     /locus_tag="ACHUPG_001923"
     CDS             complement(3125..3424)
                     /locus_tag="ACHUPG_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR0861557.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3599..4594
                     /gene="wecH"
                     /locus_tag="ACHUPG_001924"
     CDS             3599..4594
                     /gene="wecH"
                     /locus_tag="ACHUPG_001924"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR0861558.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4636..5073)
                     /gene="yiaA"
                     /locus_tag="ACHUPG_001925"
     CDS             complement(4636..5073)
                     /gene="yiaA"
                     /locus_tag="ACHUPG_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFR0861559.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5119..5460)
                     /gene="yiaB"
                     /locus_tag="ACHUPG_001926"
     CDS             complement(5119..5460)
                     /gene="yiaB"
                     /locus_tag="ACHUPG_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR0861560.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5629..7083)
                     /gene="xylB"
                     /locus_tag="ACHUPG_001927"
     CDS             complement(5629..7083)
                     /gene="xylB"
                     /locus_tag="ACHUPG_001927"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFR0861561.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7155..8477)
                     /gene="xylA"
                     /locus_tag="ACHUPG_001928"
                     /pseudo
     CDS             complement(7155..8477)
                     /gene="xylA"
                     /locus_tag="ACHUPG_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8843..9835
                     /gene="xylF"
                     /locus_tag="ACHUPG_001929"
     CDS             8843..9835
                     /gene="xylF"
                     /locus_tag="ACHUPG_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0861562.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9913..11454
                     /gene="xylG"
                     /locus_tag="ACHUPG_001930"
     CDS             9913..11454
                     /gene="xylG"
                     /locus_tag="ACHUPG_001930"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFR0861563.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11432..12613
                     /gene="xylH"
                     /locus_tag="ACHUPG_001931"
     CDS             11432..12613
                     /gene="xylH"
                     /locus_tag="ACHUPG_001931"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFR0861564.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12691..13869
                     /gene="xylR"
                     /locus_tag="ACHUPG_001932"
                     /pseudo
     CDS             12691..13869
                     /gene="xylR"
                     /locus_tag="ACHUPG_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
     gene            complement(13977..14801)
                     /locus_tag="ACHUPG_001933"
     CDS             complement(13977..14801)
                     /locus_tag="ACHUPG_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFR0861565.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15121..17151
                     /gene="malS"
                     /locus_tag="ACHUPG_001934"
     CDS             15121..17151
                     /gene="malS"
                     /locus_tag="ACHUPG_001934"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR0861566.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17329..18582
                     /gene="avtA"
                     /locus_tag="ACHUPG_001935"
     CDS             17329..18582
                     /gene="avtA"
                     /locus_tag="ACHUPG_001935"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFR0861567.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18733..19206)
                     /locus_tag="ACHUPG_001936"
     CDS             complement(18733..19206)
                     /locus_tag="ACHUPG_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR0861568.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19308..20156)
                     /gene="yiaJ"
                     /locus_tag="ACHUPG_001937"
     CDS             complement(19308..20156)
                     /gene="yiaJ"
                     /locus_tag="ACHUPG_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFR0861569.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
BASE COUNT         5069 a         5142 c         4940 g         5012 t
ORIGIN      
        1 aaagcctgcc agatggcagg ctttttaata acggcgttat tactgcaaca gcgaaatatc
       61 cgcaacgcgc aggaacagtt cgcgcagttt ctccagcatg gtcagacggt tgagacgcaa
      121 ttctttgtca tcaaccatga ccatcacttt atcgaagaaa gcatcaaccg gctcacgcag
      181 ctctgccagt tcgaccagcg catcctggta acgaccttcc gcaaagtacg gctccagctt
      241 gtcacgtagc accacaacct gcatcgccag tttaatttct tccggctctt tcagggtaga
      301 ggcattcacg cgatcgctca gcacttcgtc agatttcgcc aggatgttag atacacgttt
      361 gttcgccgcc gccagtgctg cagctgcttc cagggtacgg aagtgcgata ccgctttcat
      421 gcgggcatcg aaatcagccg gacgagtcgg acgacgcgcc agtaccgcct ggatggtgtc
      481 aacggtataa ccttcgtcct gataccaggc gcggaagcga ccgagcataa agtcgataac
      541 atcatcaact acgttggcat tagtcagctt atcgccatac agacgcaccg cttcttcggt
      601 cagcgtttgc agatcaaggt tgaggttctt ctcaacgata attcgcagca cgccaagtgc
      661 ggcacgacgc agcgcaaacg ggtctttgtc gcctttcgga tgctgaccga taccgaagat
      721 acccgccaga gtatccatct tatcagcaat cgccagcgca caagctaccg ggttggacgg
      781 caggtcatca ccggcaaagc gcggctgata ctgctcattc agcgcgacgg ctacgtcttc
      841 cgcttcgcca tcgtgacgcg cgtagtgcat ccccataacg ccttgggtgt cggtgaactc
      901 gaagaccatg ttggtcatca ggtcacactt agacagcagg cccgcacggg ttgcgtggtt
      961 aacgtcagcg ccaatctgtt cggcgatcca gccagccagc gcctggatgc ggtcagtctt
     1021 gtcacgcagc gtacccagct gttgctggaa cagcacggtt tgcaggcgcg gcaggttatc
     1081 ttccagacgt tttttgcggt cggtgttgaa gaagaactcg gcatccgcca gacgtggacg
     1141 aacgactttc tcgttaccag agataatttg ctgcggatct ttcgattcga tgttggcaac
     1201 gaagataaag ttcggcagca gtttgccgtc gttcgcatac accgggaagt acttctggtc
     1261 acctttcatg gtgtaaacca gggcttcagc cggcaccgcg aggaatttct cttcgaattt
     1321 tgcggtcaga acgaccggcc actccaccag cgaagccact tcttccagca ggctttcgct
     1381 taagtcagcg ttaccgccaa tcttacgcgc tgcttcttcg gcatcggctt taatcttcgc
     1441 tttacgttct tcgtaatcgg cgatgacttt cccgcgctca cgcagaattt ccggatactg
     1501 atcggcgttg tcgatggtga attccggttc gcccataaag cggtggccgc gaatcacgcg
     1561 atcggactga atgcccagaa tggttgccgg aatgactttg tcgcccagca gcagggtcac
     1621 ggtgtgcacc ggacgcacga agtgcacgtc gcttgcaccc cagcgcatca gtttcgggat
     1681 cggcagtttc gccagcgaag tcgcaaccat attcggcagc agtgcttcgg tgctttcgcc
     1741 cttcacatgg gcgcgataca gcagccattc gcctttatcg gtagtcagac gctcagcctg
     1801 gtcaacggta ataccacaac cacgcgccca accttctgcc gctttgctcg gttttccttc
     1861 agcgtcgaac gcctgggcaa tcgccgggcc gcgtttttcg atttcacgat ctggttgtgc
     1921 ttccgccagg ttagctactt tcagcgccag acgacgcgga gcagcaaacc attgaacggt
     1981 gccgtgtgcg aggcctgcgt tatccagctc cgcagtaaag ttcgcagcaa aggactctgc
     2041 caggctgcgc agtgcttttg gtggcagctc ttcagtgccg atttccacca gaaaagtttt
     2101 ctcagacata gccgcctctt acttatcttt gttgcacatc gggaagccga gggcttcacg
     2161 ggaagcgtaa tatgcttctg ccactgcttt ggtcagggtg cgaatgcgca gaatgtagcg
     2221 ctgacgctcg gtgacggaga tggctttacg cgcatccagc aggttgaagc tgtgggcggc
     2281 tttcagaata cgctcgtagg ctggcagcgg cagcggattt tccagcgcca gcagctgctg
     2341 cgcttctttc tcgtactgct caaagcaggt gaacaggaag tccacatccg cgtattcgaa
     2401 gttgtaagtg gactgctcca cttcgttctg atggaacacg tcgccgtagg tggttttacc
     2461 cagcgggccg tcgctccaga ctaggtcgta aacgctgtct acgccctgaa tgtacatggc
     2521 cagacgttcc agaccgtagg tgatctcgcc ggtaaccggt ttacactcca gaccaccaac
     2581 ctgctggaag taagtgaact gcgtcacttc catgccgttt agccacactt cccagcccag
     2641 tccccaggcc cccagagtcg ggttttccca gttatcttcc acgaaacgga tgtcgtgaat
     2701 agtcgggtcc atgcccagct ctttcagaga accgaggtac agctcctgaa tattgtccgg
     2761 cgatggctta atgaccacct ggaactgata gtagtgctgt aaacggttgg ggttttcgcc
     2821 gtagcgacca tcggtcgggc gacgagaagg ctgaacataa gcagccgcca tcggttctgg
     2881 ccccagcgcg cgcagacagg tcattgggtg agaggttccc gcgccgactt ccatgtccaa
     2941 tggttgaaca atggtgcagc cctggcgagc ccagtaatcc tgtaaggtca ggatcaagcc
     3001 ctggaaggtc ctggtatcaa acttttgcat attatttcgt gctggatacg tgtggattta
     3061 aaggaaggga tcagtatacc cgctggatgg aagatataca gtacgaaacg ggaaaaagca
     3121 gggcttaacg catggaaaga tgcaaaaaat ggccatctgc gtcaaaagaa cagacaaact
     3181 gctctttacg gaaggtataa ccgcgcattt catagcttcc ctggaactgt tcgaaggcgg
     3241 tgacgtcgat tttttgctcg ccggtgttat aacgttttgc cgcctgatcc ttgcataacg
     3301 cttccatatt caacgaacgc agaggatcaa cccttacccg ttgtgctttc tgcacgggtg
     3361 acggtgtaga acaacccact agcaccataa gcgccattgc cggaaagaaa gcgttcatca
     3421 tcattaaatt accgtctggt ttgcaggtga gtcttattat ttatatcggt atagtggctg
     3481 atatcttacg aatttcctta agcctctata ggctgtagtg ataaaactca gattattcca
     3541 gtgcgccaac caaatgattt atcggtcaga ctttttttaa ccagatacag agacacagat
     3601 gcagcccaaa atttactgga ttgataacct gcgagggata gcgtgtttaa tggtggtgat
     3661 gattcacacc actacctggt atgtgaccaa tgctcatagt gttagccccg tcacatggga
     3721 tatcgccaat gttctgaatt ctgcctctcg tgtcagcgtg ccgctatttt tcatgatttc
     3781 cggctatctc ttttttggcg aacgcagcgc ccagccgcgc catttcttgc gtatcggctt
     3841 atgtctgatt ttttatagcg caatcgcact gctctacatt gcgctgttta cctccatcaa
     3901 tatggagtta gcgctgaaaa acctgctgca aaagccagtg ttttaccact tgtggttttt
     3961 cttcgcgatt gcggtgattt atctggtttc accgctgatt caggtgaaga acgtcggcgg
     4021 aaaaatgttg ctggtgctaa tggtggtgat tggcattatc gctaacccaa acacagtgcc
     4081 gcagaaaatt gacggttttg aatggctgcc aattaactta tatatcaatg gcgatacttt
     4141 ttactacatt ctgtatggca tgttgggccg cgctataggg atgatggaca cacagcataa
     4201 agcactgtcg tgggtgagcg ccgcgctgtt tgcgacgggg gtttttatta tctctcgcgg
     4261 gacattatat gaattgcagt ggcgcggaaa ttttgccgat acctggtatc tttactgtgg
     4321 gccgatggtt tttatctgcg caatcgcgct attgactctg gttaaaaaca cgctggatac
     4381 gcgtaccatt cgcggacttg gcttaatctc ccgccattca ttgggtatat acggattcca
     4441 cgccttgatt atccatgcgc tgcgcacccg gggaattgag cttaaaaatt ggccaatact
     4501 ggatattatt tggatctttt gcgcgacgtt ggcagcgagt ttgttacttt ctatgctggt
     4561 acaacgaatc gacagaaaca gattagtgag ttaagtaaaa gcccgttcac attggactga
     4621 ccgggcttac gtgaattatt cgctgtattc ttcctgggta acctgtgttt cttttggcgg
     4681 gtttattccg gcatcacgaa tattcttctg caccgctact gcaccaaaca agcttaagaa
     4741 gaaagccagt ccataaaaac ctttttcact gagtaataat gtcgcattcc acagacctac
     4801 catcagtaga gcaacagaga taataaagac agtcaggcag gtcatataat aaatggaagt
     4861 ggtcggtatg ccttcatact tgtcccgaac ggtcttttga taagacgcag cagaaaacag
     4921 tcctaacacc agtacggcaa aataataccc tttttcattt aactgcatct ctgcattcca
     4981 tagccctaac aggtaggtaa cgataccacc aacgagagct atccatgaca caatactaaa
     5041 ggccggtgaa taggttgata ttttgttgtc catcacagta ttcctttcat tcctgaatgt
     5101 gtaagagctt tatgttgctc ataccgagcg aaacaaacgc atttgaccaa acaagcagac
     5161 aaaaaaggcg atggggtaga tcaccatttc ataagcattg ataggtgcat ttaaaacacc
     5221 gataaacaag agaccgatag tgattaacgc aactaactgg cagatatggg taaaaaaatt
     5281 atccagttgc ccgagttttt ctgcgcgaag atatgcatag ttaccaaaag ttgctgtcat
     5341 taacacgcca agaaaatagc cttttccact tagcaatggg catgagatcc atagcccaac
     5401 cagataaacc agcgccccga caacaaataa tagttttgcc acagtttttg aggttttcat
     5461 aacgatctcc atatctacca gcgatacatt acgagtaacc aacgaaagac aaaactgaaa
     5521 aatgccatta acaaatgatt ttcagaataa attcatacta aatattaatt aattagtgag
     5581 atatatagat gtgaattatc ccccacccgg tcaggcaggg gataacgttt acgccattaa
     5641 tggcagaagt tgctgataga ggcgacggaa cgtttcacgt cgtggctgat aagcggcata
     5701 acgctgcgca tctggtagat gcgactgttc taacggtagc tgcggcaaca actcaatgag
     5761 cgatttctct ggattcgccg caatctgcgc cagccttgct gcgcccagtg ctggccccac
     5821 atcccccccc gtacggtaat cgagctgctg accgctgata tccgccagca tctgacgcca
     5881 gtactcacta cgcgccccgc caccaatcaa cgtaacactt tgcggtttaa tgccgcaggc
     5941 atgcacgaca tccatgccat ctgccagcgc ataacccacg ccttccagca ctgctcgcgc
     6001 cagttcattg gggccatgtt gatgagtcaa accaaagaaa acccccttcg cctggggatt
     6061 attgtgtggt gtacgctcgc cggaaagata aggcagaaac caaactggct cggcactttc
     6121 atcagcctgt tgagctgcag cgattaaagc tgggacattg cacaggccgg ttaatttcgc
     6181 ggcccaatcc agacacgatg ctgcactcag cattacagac attaaatgcc aacgctgcgg
     6241 tagcgcatgg caaaagctgt gtacagcgct ttctggcttg cttaagaacc cttcgctgac
     6301 agcaaaatag acccccgacg tccccagcga taacattgcc tgattagcat caaccattcc
     6361 cacaccaact gcaccagctg cattgtcgcc accgcctgcg acaactggca ccgtcgccat
     6421 accccacgct ttcgcaactt caggtaacaa agcaccagta atttcgctgc cttcgtataa
     6481 tgcgggcatc tggtcacgag ataagtcgca agcctgcagc atgacgtcac tccagtcacg
     6541 ctttgcgaca tccagccaca tggtgccagc tgcgtcagac atatcgctgg caaactcccc
     6601 cgtcatacgc agacgcaagt aatctttcgg taataatact ttgtcgattt gacggaatat
     6661 ctccggctca tgccgctgaa cccatagcaa tttaggcgca gtaaatccgg gcatcatcag
     6721 gttgccggta atcactcgtg attgcggaac tctcgcttcc agcaaagtgc actcttgcgc
     6781 acagcgcccg tcgttccaca aaatggcagg gcgcaatacc cgttgttgag catccagtaa
     6841 ggttgctcca tgcatctggc cggcaatacc caatgcttta acgtcctgca gagaatgctg
     6901 atcgcccaga gctttcattg cgcgatcagt tgcctgccac cactgttccg ggtcttgttc
     6961 cgaccaaagt ggatgcgggc gcgaaacggt cagcttttcc gtttgcgaag caaccacctc
     7021 accctgctcg ttgagcaaaa taacttttac gcccgaggtg ccaagatcta tcccgatata
     7081 catatcgatc gttccttaaa aaaatgcccg gtatcgctac cgataaccgg gccaacggac
     7141 tgcacagtta gccgttattt gtcgaacaga taatgattta ccagattttc cagttgctcc
     7201 tggcgaccac tctgatgcac cggagacaaa ttatgttcct gagcatattt ggctaaatct
     7261 gccagtgaca tttggccttt caggatttgc tggcccaatt cgctattcca gccggaataa
     7321 cgctgcgcga tgcgtttatc cagctcgcca tcttcaatca tgcgcgctgc aattttcagc
     7381 gccagtgcca tcgtatccat cgcgccgata tgaccgtaaa acagatcata tttatcagta
     7441 ctttgacgac gtactttggc atcgaagttc agaccaccgg tggtgaaacc gcctgctttg
     7501 agaatttcat acatcaccag cgcattctct tccacactgt tcgggaactg gtcggtgtcc
     7561 cagcccagtt gcgcatcgcc acggttggcg tcgacagaac cgaacaggcc aagcgcaatg
     7621 gcggtggcta tttcatgatg gaaagagtga cctgccagcg tcgcgtggtt agcttcaatg
     7681 ttcagtttaa tctctttttc cagaccaaac tgtttcagga agccatagac cgtcgcggca
     7741 tcgtaatcat attgatgttt agtcggttct tgcggtttcg gttcgataag caacgtgccc
     7801 tagaagccga ttttatgttt atgctcaacc accatctgca taaagcggcc cagttgttca
     7861 cgctcctgac gcaagtcggt atttaacagc gtttcgtaac cttcacgacc accccacagg
     7921 acatagtttt caccgcccaa tttatgggtt gcttccatcg ctgtaacaac ttgcgttgcc
     7981 gcccagctga agacttcagg atctgggttc gtcgccgcac ccgcgccata gcgagggttt
     8041 gtaaagcagt tagcggttcc ccacagcagc ttcacgctgc tctcttcttg cttggctgcc
     8101 aggacatcaa ccatttgcgc aaaattattg atgtactctt ttaacgacgc gccctcaggg
     8161 gaaacatcca catcgtggaa gcaataaaat ggcacatgta acttgtggaa aaactcaaat
     8221 gcgacatctg ctttacgctt cgccaacgcc agtgcctcac caggctgctg ccacggacga
     8281 ttaaacgccc ccacaccaaa catgtccgcc ccgttccagc agaaggtgtg ccagtagcag
     8341 gcggcaaaac gcaagtgctc ttccatacgc ttacccaaca ccagttcgtc gggattgtag
     8401 tgacggaatg ctaacgggtt tgaggatttt gagccttcat aacgaacgcg atcgagctgg
     8461 tcaaaatagg cttgcatatt gaactccata atcaggtaat gccgcgggtg atggatgatg
     8521 tcgtaatatt gggcactccc tttcaattgc tcaattatgt tatttcacac tgctattgag
     8581 ataattcaca agtgtgcgct cgctcgcaaa ataaaatggg atgatgaaaa tgggtaattc
     8641 ctcgaagata aaaatgcaat aagtacaatt gcgcaacaaa agtaagatct cggtcataaa
     8701 tcaagaaata aaccaaaaat cgtaatcgaa agataaaaat ctgtaattgt tttcccctgt
     8761 ttagttgcta aaaattggtt acgtttatcg cggtgattgt tacttattaa agctgtcctc
     8821 taactacaga aggccctaca ccatgaaaat aaagaacatt ctactcaccc tttgcacctc
     8881 acttctgctt accaacgttg cggcacacgc caaagaagtc aaaataggta tggcgattga
     8941 tgatctccgt cttgaacgct ggcaaaaaga tcgagatatt tttgtgaaaa aggcagaatc
     9001 tctcggtgcg aaagtatttg tacagtctgc aaatggcaat gaagaaacac aaatgtcgca
     9061 gattgaaaac atgattaacc ggggtgtcga tgttcttgtc attattccgt ataacggtca
     9121 ggtattaagt aacgttgtaa aaaaagccaa acaagaaggt attaaagtat tagcttacga
     9181 ccgtatgatt aacgatgcgg atatcgattt ttatatttct ttcgataacg aaaaagtcgg
     9241 tgaactgcag gcaaaagcct tggtcgatat tgttccgcaa ggtaattact tcctgatggg
     9301 cggctcgccg gtagataaca acgccaagct gttccgcgcc ggacaaatga aagtgttaaa
     9361 accttacgtt gattctggaa aaattaaagt cgttggtgac caatgggttg atggctggtt
     9421 accggaaaac gcattgaaaa ttatggaaaa cgcgctaacc gccaataata acaaaattga
     9481 tgctgtagtt gcctcaaacg atgccaccgc aggtggggca attcaggcat taagcgcgca
     9541 aggtttatca gggaaagtag caatttccgg ccaggatgcg gatctcgcag gtattaaacg
     9601 tattgctgcc ggtacgcaaa ctatgacggt gtataaacct attacattgc tggcaaatac
     9661 tgccgcagaa attgccgttg aattgggcaa tggtcaggag ccaaaagcag atacctcact
     9721 gaataatggc ctgaaagatg tcccctcccg cctcctgaca ccgatcgatg tgaataaaaa
     9781 caacatcaaa gatacggtaa ttaaagacgg attccacaaa gagagcgagc tgtaagcgtt
     9841 acgccccggc gcggagcggg ggcgtgattt ctctccatgc cgcgtgaatg aattggctta
     9901 ggtggagtcg ttatgcctta tctacttgaa atgaagaaca ttaccaaaac cttcggcagt
     9961 gtgaaggcga ttgataacgt cagcttgcgg ttgaatgctg gcgaaatcgt ctcactctgt
    10021 ggggaaaatg ggtctggtaa atcaacgctg atgaaagtgc tgtgtggtat ttatccccat
    10081 ggctcctacg aaggcgaaat tatttttgcg ggagaagaga ttcaggcgag tcacatccgc
    10141 gataccgaac gcaaaggtat cgccatcatt caccaggaat tggccctggt gaaagaattg
    10201 accgtgctgg aaaatatctt cctgggtaac gaaataaccc acaatggcat tatggattat
    10261 gacctgatga cgctacgcta tcagaagctg ctcgcacagg tcagtttatc catttcacct
    10321 gatacccgcg ttggcgattt agggcttggg caacaacaac tggttgaaat tgccaaggca
    10381 cttaataaac aggtgcgctt gttaattctc gatgaaccga cagcctcatt aactgagcag
    10441 gaaacgtcgg ttttactgga tattattcgc gatctacaac agcacggtat cgcctgtatt
    10501 tatatttcgc acaaactcaa cgaagtcaaa gcgatttccg atacgatttg cgttattcgc
    10561 gacggacagc acattggtac gcgtgatgct gccggaatga gtgaagacga tattatcacc
    10621 atgatggtcg agcgagagtt aaccgcgatt taccctaatg aaccacatac caccggagat
    10681 gaaatattac gtattgaaca tctgacggca tggcatccgg tcaatcgtca tattaaacga
    10741 gttaatgatg tctcgttttc cctgaaacgt ggcgaaatac tgggtattgc cggactcgtt
    10801 ggtgccggac gtaccgagac cattcagtgc ctgtttggcg tgtggcccgg acaatgggaa
    10861 ggaaaaattt atattgatgg caaacaggta gatattcgta actgtcagca agccatcacc
    10921 caggggattg cgatggtacc cgaagacaga aagcgcgacg gcatcgttcc ggtaatggcg
    10981 gttggtaaaa atattaccct cgccgcactc aataaattta ccggtggcat tagccagctt
    11041 gatgacgcgg cagagcaaaa atgtattctg gaatcaattc agcaactcaa agttaaaacg
    11101 tcgtcccccg accttgctat tggacgtttg agcggcggca atcagcaaaa agcgatcctc
    11161 gcccgctgtc tgttacttaa cccgcgcatc ctcattcttg atgaacctac caggggtatt
    11221 gatattggcg cgaaatatga gatctacaaa ttaattaacc aactcgtcca gcagggtatt
    11281 gccgttattg tcatctcttc cgaattacct gaagtgctcg gccttagcga tcgtgtactg
    11341 gtgatgcatg aagggaaact aaaagccaac ctgataaatc ataacctgac tcaggagcag
    11401 gtgatggaag ccgcattgag gagcgaacat catgtcgaaa agcaatccgt ctgaagtgaa
    11461 attggccgta ccgacatccg gtggcttttc tgggctgaaa tcactgaatt tgcaggtctt
    11521 cgtgatgatt gcagctatca tcgcaatcat gctgttcttt acctggacca ccgatggtgc
    11581 ctacttaagc gcccgtaacg tctccaacct gttacgccag accgcgatta ccggcatcct
    11641 cgcggtagga atggtgttcg tcataatttc tgctgaaatc gacctttccg tcggctcaat
    11701 gatggggcta ttaggtggcg tcgcggcgat ttgtgacgtc tggttaggct ggcctttgcc
    11761 acttaccatc attgtgacgt tggttctggg actgcttctc ggtgcctgga acggatggtg
    11821 ggtcgcgtac cgcaaagtcc cttcatttat tgtcaccctc gcgggcatgt tggcatttcg
    11881 cggcatactc attggcatca ccaacggcac gactgtatcc cccaccagcg ccgcgatgtc
    11941 acaaattggg caaagctatc tccccgccag taccggcttc atcattggcg cgcttggctt
    12001 aatggctttt gttggttggc aatggcgcgg aagaatgcgc cgtcaggctt tgggtttaca
    12061 gtctccggcc tctaccgcag tagtcggtcg ccaagcttta accgctatca tcgtattagg
    12121 cgcaatctgg ctgttgaatg attaccgtgg cgttcccact cctgttctgc tgctgacgtt
    12181 gctgttactc ggcggaatgt ttatggcaac gcggacggca tttggacgac gcatttatgc
    12241 catcggcggc aatctggaag cagcacgtct ctccgggatt aacgttgaac gcaccaaact
    12301 tgccgtgttc gcgattaacg gattaatggt agccatcgcc ggattaatcc ttagttctcg
    12361 acttggcgct ggttcacctt ctgcgggaaa tatcgccgaa ctggacgcaa ttgcagcatg
    12421 cgtgattggc ggcaccagcc tggctggcgg tgtgggaagc gttgccggag cagtaatggg
    12481 ggcatttatc atggcttcac tggataacgg catgagtatg atggatgtac cgaccttctg
    12541 gcagtatatc gttaaaggtg cgattctgtt gctggcagta tggatggact ccgcaaccaa
    12601 acgccgttct tgattttgat aaaaattttc tcaaagccgg ttacgtatta ccggttttga
    12661 gtttttgcat gattcagcag gaaaagaacc atgtttacta aacgtcaccg catcacatta
    12721 ctgttcaatg ccaataaagc ctatgaccgg caggtagtag aaggcgtagg ggaatattta
    12781 caggcatcac aatcagaatg ggatattttc attgaagaag atttccgcgc ccgcattgat
    12841 aaaatcaagg actggttagg agatgtcgtc attgccgact tcgacgacaa acagatcgag
    12901 caagcgctgg ctgatgtcga cgtccccatt gttggggttg gcggctcgta tcaccttgca
    12961 gaaagttacc cacccgttca ttacattgcc accgataact atgcgctggt tgaaagcgca
    13021 tttttgcatt taaaagagaa aggcgttaac cgctttgctt tttatggtct tccggaatca
    13081 agcggcaaac gttgggccac tgaacgcgaa tatgcatttc gtcagcttgt cgccgaagaa
    13141 aagtatcgcg gagtggttta tcaggggtta gaaaccgcgc cagagaactg gcaacacgcg
    13201 caaaatcggc tggcagactg actacaaacg ctgccaccgc aaaccgggat tattgccgtt
    13261 actgacgccc gagcgcggca tattctgcaa gtatgtgaac atctacatat tcccgtaccg
    13321 gaaaaattat gcgtgattgg catcgataac gaagaactga cccgctatct gtcgcgtgtc
    13381 gccctttctt cggtcgctca gggcgcgcgg caaatgggct atcaggcggc aaaactgttg
    13441 catcgattat tagataaaga agaaatgccg ctacagcgaa ttttggtccc accagttcgc
    13501 gtcattgaac ggcgctcaac agattatcgc tcgctgaccg atcccgccgt tattcaggcc
    13561 atgcattaca ttcgtaatca cgcctgtaaa gggattaaag tggatcaggt actggatgcg
    13621 gtcgggatct cgcgctccaa tcttgagaag cgttttaaag aagaggtggg tgaaaccatc
    13681 catgcaatga ttcatgccga gaagctggag aaagcgcgca gtctgctgat ttcaaccacc
    13741 ttgtcgatca atgagatatc gcaaatgtgc ggttatccat cgctgcaata tttctactct
    13801 gtttttaaaa aagcatatga cacaacgcca aaagagtatc gcgatgtaaa tagcgaggtc
    13861 atgttgtagc gatgatgaga attgtcggcg tcacatcagg gaactcagca cagccacctg
    13921 atgcgagcaa aagccttatc cgaccgacaa atcagataag gctcgaggaa atgaaattac
    13981 atatgcgccg cgattaaccg ttggttatcc tggtacattg cgaacaggta gttgttataa
    14041 ctcttccctt tggtcgagta gcccttcagc ttgtgaatca tcgctgtggc agtcacttcc
    14101 tgatccgctt tacgcagctg tgcacgcgat ttacggaacg aagagtaagc cgggtgcgta
    14161 ttcaggttag tgacataggc gctcaccgat tctttgacag aactaaactg tgagtaccct
    14221 ttcactttac ccggcgcatt ggtacaacgt cctttcatgc atttcatgcc gaacagattg
    14281 ttattgttgc gcgccagctt cgacgttccc caaccgcttt ctgctgcagc catcgtcgcc
    14341 accatactgg tagggataat gtctacgcgt tcaagcaagg tattccacgg gatttttcgc
    14401 gtattaccag accacttcac cttgtagcgt ttggcgatgt ctttcagacg cgcacgctca
    14461 gcaggtgccc attggccctg atactgtttt gaaatcagcc agttacgttc tgcagtaatg
    14521 gctgcatttt ggctggtaat gtaaggcatt acggtccgga gaaacgcctt tttccttggt
    14581 gttccggaag ggtattttcg caaatcagga agtgaactac tctttgcact attgcgagaa
    14641 tactcttgtt tactgcttac ctgtttatta ctagctttag ttaagtggga cttttgactc
    14701 gctgttgttg tgtgcgtctt cgctaacacc tcactcgaaa acaccagagt gagtaacata
    14761 agaatcatcg ccccatatcg tcgtatggga gtcaaaatca tcaggtctcc tggtcggatt
    14821 taatcattcc aacaccttat atttttcaca aatttgagag ttgaatctca aatcatatca
    14881 aaaatagctg tcaagagcac cccaaggaat agtccaaatc tgaaactatg tcacgagtta
    14941 acgattcaga ttggcgctaa atcgcagaaa atgtgggggt tatcgcaaaa ttcagccgtt
    15001 ttttgcgcgc gatcgctcac ccttgcttct catcctgtgg acttaccgct cagggatgag
    15061 ttttgtctgg cttatcgctg gcaaactgtc tgaaatcgca gcaataagga ctcatccacc
    15121 atgaaactcg ccgcctgttt tctgacactc cttcctggct tcgccgttgc cgccagctgg
    15181 acttctccgg ggttccctgc ctttagcgaa cagggaacgg gaacatttgt cagccacgcg
    15241 cagttgccca aaggtacgcg tccactcacg ctaaattttg accaacagtg ctggcagcct
    15301 gcagatgcga taaaactcaa tcagatgctt tccctgcaac cttgtagcaa cacgccgcct
    15361 caatggcgat tgttcaggga cggcaaatat acgctgcaaa tagacacccg ctccggtacg
    15421 ccaacattga tgatttccat ccagaacgcc gccgaaccgg tagcaaacct ggtccgtgaa
    15481 tgcccgaaat gggatggatt accgctcacg ctggatgtca gcgccacttt cccggaagga
    15541 gccgccgtac gggattatta cagccaacaa attgcgatag tgaagaacgg tcaaataacg
    15601 ttacaacccg ctgctaccag caacggttta ctcctgctgg aacgggcaga aactgacaca
    15661 tccgcccctt tcgactggca taacgccacg gtttactttg tgctgacaga tcgtttcgaa
    15721 aacggcgatc ccagtaatga ccagagttac ggacgtcata aagacggtat ggcggaaatt
    15781 ggcacttttc acggcggcga tttacgcggc ctgaccaaca aactggatta cctccagcag
    15841 ttgggcgtta atgctttatg gataagcgcc ccatttgagc aaattcacgg ctgggtcggc
    15901 ggtggtacaa aaggcgattt cccgcattat gcctaccacg gttattacac acaggactgg
    15961 acgaatcttg atgccaatat gggcaacgaa gccgatctac ggacgctggt tgatagcgca
    16021 catcagcgcg gtattcgtat tctctttgat gtcgtgatga accacaccag ctatgccacg
    16081 ctggcggata tgcaggagta tcagtttggc gcgttatatc tttctggtga cgaagtgaaa
    16141 aaaacgctgg gtgaacgctg gagcgactgg aaacctgccg ccggacaaac ctggcatagc
    16201 tttaacgatt acattaattt cagcgacaaa acaggctggg ataaatggtg gggaaaaaac
    16261 tggatcagaa cggatatcgg cgattacgac aatcctggat tcgatgatct cactatgtcg
    16321 ctggcctttt tgccggatat caaaaccgaa tcaactaccg cttctggtct gccggtgttc
    16381 tataaaaaca aaacggatac tcacgctaaa gtcatcgaag gctttacacc tcgcgattac
    16441 ttaacccact ggttaagtca gtgggtccgc gactatggga ttgatggttt tcgggtcgat
    16501 accgccaaac atgttgagtt gcccgcctgg cagcaactga aaaccgaagc cagctccgcg
    16561 cttcgcgaat ggaaaaaaac taaccccgac aaagcattag atgacaaacc tttctggatg
    16621 accggtgaag cctggggcca cggcgtgatg caaagtgact actatcgcca cggcttcgat
    16681 gcgatgatca atttcgatta tcaggagcag gcggcgaaag cagtcgattg tctggcgcag
    16741 atggatacga cctggcagca aatggcggag aaattgcagg gtttcaacgt gttgagctac
    16801 ctctcgtcgc atgatacccg tctgttccgt gaagggggcg acaaagcagc agagttatta
    16861 ctattagcgc caggcgcggt acaaatcttt tatggcgatg aatcctcgcg tccgttcggt
    16921 cctacaggtt ctgatccgct gcaaggtaca cgttcggata tgaactggca ggatgttagc
    16981 ggtaaatctg ccgccaacgt cgcgcactgg cagaaaatca gccagttccg cgcccgccat
    17041 cccgcaattg gcgcgggcaa acaaacgaca ctttcgctga agcagggcta cggctttgtt
    17101 cgtgagcatg gcgacgataa agtgctggtc atctgggctg ggcaacagtg acttttccgg
    17161 cttcccgttc gtcagtacct cgggaagccg ccaaccagga taaaatgtca gccctaatca
    17221 gcgttgcggg ataaagcacc gctcactctt caacagaccg atttgcaccc cagcaaatgt
    17281 agcgttattg ttaccttcct tgctacagag ttcgacagat atcccgctat gacattctcc
    17341 ctttttggtg acaaatttac ccgccactcc ggcattacgc tgctgatgga agatctgaac
    17401 gacggtttac gcacgcctgg cgcgattatg ctcggcggcg gtaatccggc gcagatcccg
    17461 gaaatgcagg actacttcca gacgctactg accgacatgc tggaaagtgg caaagcgact
    17521 gatgcactgt gtaactacga cggtccacag gggaaaacgg agctactcac actgcttgcc
    17581 ggaatgctgc gcgagaagtt gggttgggat atcgaaccac agaatattgc actaacaaac
    17641 ggcagccaga gcgcgttttt ctacttattt aacctgtttg ccggacgccg tgccgatggt
    17701 cgggtcaaaa aagtgctgtt cccgctggca ccggaataca ttggctacgc tgacgccgga
    17761 ctggaagaag atctgtttgt ctctgcgcgt ccgaatattg aactgctgcc ggaaggccag
    17821 tttaaatacc acgtcgattt tgagcatctg catattggcg aagaaaccgg gatgatttgc
    17881 gtctcccggc cgacgaatcc aacaggtaat gtgattactg acgaagagtt gctgaagctt
    17941 gacgcgctgg cgaatcaaca cggcattccg ctggtgattg ataacgctta tggcgtcccg
    18001 ttcccgggta tcatcttcag tgaagggcgc ccgctatgga atccgaatat cgtactgtgc
    18061 atgagtcttt ccaagctggg gctgcctggc tcccgctgcg gcattatcat cgccaatgaa
    18121 aaaatcatca ctgccatcac caatatgaac ggcattatca gcctggcacc tggcggtatt
    18181 ggtccggcga tgatgtgtga aatgattaag cgtaacgatc tgctgcgcct gtctgaaaca
    18241 gtcatcaaac cgttttacta ccagcgtgtt caggaaacta tcgccatcat tcgccgctat
    18301 ttaccggaaa atcgctgcct gattcataaa ccggaaggag ccattttcct ctggctatgg
    18361 tttaaggatt tgcccattac gaccgagcag ctctatcagc gcctgaaagc acgcggcgtg
    18421 ctgatggtgc cggggcacaa cttcttcccg ggactggata aaccgtggcc gcatacgcat
    18481 caatgtatgc gcatgaacta cgtaccagag ccggagaaaa ttgaggcggg ggtgaagatt
    18541 ctggcggaag agatagaaag agcctgggct gaaagtcact aaaaattgcc tgatgcgcta
    18601 cgcttatcag gcctacatga tttctgcaat atattgaatt tggaagaatt tgtaggccgg
    18661 ataaggcgtt tacgccgcat ctggcataaa caaagcgcac tattccggca ttgagaaacg
    18721 ccggaaaacg tttcaaacag gctgctgctg tagccgatgc cgctgcactt tctcgacatc
    18781 catgcactgc aacgcctttg tcgggcaggc ttcaacacag gccggccccg tctcccgatg
    18841 ccagcacaga tcgcacttaa tcgccctcgc ctttttgcgc gaagaaacga cctccatcgc
    18901 accaaacggg caagccagca tacagctttt acagccaatg caacgtgttt gttcaacgaa
    18961 aatatgccca tgttcgcggc ttatcgcgtc aacagggcag acattcgcgc acggtgcatc
    19021 ttcacactga tgacaggcta ctggcgtggt ccagctgtgg tctttaatga cacgaatacg
    19081 ggaaataaac tcgtctggtg acaacgcagc gcaatcctga ttctcctgat gcgacactgc
    19141 gcaagccact tcacaggtac ggcaaccgat acatttcgtc gcatccgcaa taataaaccg
    19201 gttcatgatc attcctttgc actaaaacag cagagtgcca gagagcgcgt ccctactgct
    19261 ttgatccaac gaaggaaatg ttaaagtttt ggcacttgtc caaagcgtta tgtgatcgcg
    19321 cccaggtcat ccctgacagt aaatcccagt tcattagaaa tagcctgcgc ggtttcacgc
    19381 agtggtttca ggagattttt ctctcccacc tgtttcagac gtgatgtcga aagcgaaatc
    19441 gacacggcgt acggcacccg cccatgaata tcaaacaccg gaacagcaat acaggagacg
    19501 ccgagttcgt tttcttctct gtccatcgcc gctccgcttt cacgaatgtg cgccagttcg
    19561 tcgaacatcg cgggcagctc ggtaatggta ttgcgggtta acggctggat ctcatgctga
    19621 tggttttccc agtatgactt cacgtagtcc gggtgaccaa acgccatgta gatcttgccc
    19681 attgcggaac agtagagcgg catatgctgg ccaatatagg cacgggttcg cagcatcccg
    19741 gttgtgggtt ccagcttata aatcaaaata gcgtgatcgt cttcgcggct ggagaagtta
    19801 atggtttcac cagtggcgat gttcagtgcc tcaagatgcg gagcggcgat atgaatgata
    19861 ttcagcgaag acagcgcctt ctgcccgacg gcaataaatt tggtagtcag gcggtaactc
    19921 cctgcggcgg gcgcggtggt cacatagccg caggactgta atccctgcaa taagcgatgg
    19981 acggtactct tatttaaacc agccagctcc gaaagatgcg ccaacggaca accgtttgga
    20041 tagttgctca aaatctcaat cagcatcaac ccgcgaaaca gactctggct tccggctgga
    20101 cgctcttttt cctgcgccat ctcgttctct ttttttccca tcacttcttt ccccattttg
    20161 tcg
//
LOCUS       DAZPAB010000061        19998 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZPAB010000061 DAZPAB010000000
VERSION     DAZPAB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19998
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.061"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(9..221)
                     /gene="cspA"
                     /locus_tag="ACHUPG_001938"
     CDS             complement(9..221)
                     /gene="cspA"
                     /locus_tag="ACHUPG_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR0861570.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(502..792)
                     /locus_tag="ACHUPG_001939"
                     /pseudo
     CDS             complement(502..792)
                     /locus_tag="ACHUPG_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1226..1936
                     /locus_tag="ACHUPG_001940"
     CDS             1226..1936
                     /locus_tag="ACHUPG_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR0861571.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(1986..2960)
                     /gene="ghrB"
                     /locus_tag="ACHUPG_001941"
     CDS             complement(1986..2960)
                     /gene="ghrB"
                     /locus_tag="ACHUPG_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR0861572.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3064..3724)
                     /locus_tag="ACHUPG_001942"
                     /pseudo
     CDS             complement(3064..3724)
                     /locus_tag="ACHUPG_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
     gene            3877..6075
                     /locus_tag="ACHUPG_001943"
     CDS             3877..6075
                     /locus_tag="ACHUPG_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFR0861573.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6044..6484)
                     /locus_tag="ACHUPG_001944"
     CDS             complement(6044..6484)
                     /locus_tag="ACHUPG_001944"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR0861574.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6481..7044)
                     /gene="tag"
                     /locus_tag="ACHUPG_001945"
     CDS             complement(6481..7044)
                     /gene="tag"
                     /locus_tag="ACHUPG_001945"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR0861575.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7202..7900
                     /locus_tag="ACHUPG_001946"
     CDS             7202..7900
                     /locus_tag="ACHUPG_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR0861576.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8129..9337
                     /locus_tag="ACHUPG_001947"
     CDS             8129..9337
                     /locus_tag="ACHUPG_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0861577.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            9661..11352
                     /gene="eptB"
                     /locus_tag="ACHUPG_001948"
     CDS             9661..11352
                     /gene="eptB"
                     /locus_tag="ACHUPG_001948"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR0861578.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11444..11520
                     /locus_tag="ACHUPG_001949"
     tRNA            11444..11520
                     /locus_tag="ACHUPG_001949"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11478..11480,aa:Pro,seq:cgg)
     gene            12264..13871
                     /gene="dppA"
                     /locus_tag="ACHUPG_001950"
     CDS             12264..13871
                     /gene="dppA"
                     /locus_tag="ACHUPG_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR0861579.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14179..15198
                     /gene="dppB"
                     /locus_tag="ACHUPG_001951"
     CDS             14179..15198
                     /gene="dppB"
                     /locus_tag="ACHUPG_001951"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR0861580.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15208..16110
                     /gene="dppC"
                     /locus_tag="ACHUPG_001952"
     CDS             15208..16110
                     /gene="dppC"
                     /locus_tag="ACHUPG_001952"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR0861581.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16121..17104
                     /gene="dppD"
                     /locus_tag="ACHUPG_001953"
     CDS             16121..17104
                     /gene="dppD"
                     /locus_tag="ACHUPG_001953"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR0861582.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17101..18105
                     /gene="dppF"
                     /locus_tag="ACHUPG_001954"
     CDS             17101..18105
                     /gene="dppF"
                     /locus_tag="ACHUPG_001954"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFR0861583.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18135..19406)
                     /locus_tag="ACHUPG_001955"
     CDS             complement(18135..19406)
                     /locus_tag="ACHUPG_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR0861584.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            19882..19989
                     /locus_tag="ACHUPG_001956"
     CDS             19882..19989
                     /locus_tag="ACHUPG_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0861585.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         4769 a         4973 c         5291 g         4965 t
ORIGIN      
        1 agcagagatt acaggctggt tacgttacca gctgccgggc ctttagcgcc gctttcgatg
       61 gtgaaggaca ctttctgacc ttcgtccaga gatttgtaac catcgttctg gatagcagag
      121 aagtgtacga acacatcttt agagccatcg tcaggagtga tgaagccgaa gcctttgtca
      181 gcgttgaacc attttacgat accagtcatt ttaccggaca tagtgtatta cctttaataa
      241 ttaagtgtgc ctttcggcga tatggcgtgc tttacagatt ttgaagcgtt aaaggaatgt
      301 gcactacgag gggtatcaac gataactctt gaagggactt gccttactac actgctttaa
      361 tggtctgtac gtcaaaccgt tgatgtgcat taagccactc attggcgggt gatgcaacaa
      421 ttatttttca tatttatgat taatcggttg gaaaacggtc ctgtcatcag gaccgtaaac
      481 agcaataaag tggataaaag tctatttcat caactgctta cttaatgccg ggttggcttg
      541 aatcaaacgc atcaatttta gttcggcact tgaaggcttc acgcgtctgg attcccattc
      601 ctttaccatg gcgactgata cgcccaaaac ccgggcgaaa tcatcgattt ttaatcctgt
      661 cccttttcat aactgctcaa tttcggtaca ggacgttgtt ctgtgcgtca gggtaatttt
      721 ctgcgtttca tctttaaaaa caatctgttc caggctgctc aacagctcat gcattggatc
      781 tttatattcc attgaaaact cctcaaatca cactgcggga tcgtgaattg catcgaagct
      841 cattaagaat agtcgggaaa agcagacaca gcggggtgaa gtcagggcat gctctggcta
      901 tgattcgatt cagcctgtca tttagcgtga cttatagcca cctaacgatc tgataaatgc
      961 cgatatatct cttatcactc ctgttttgta aagatttcgc aaatctaacc ttctgcgaag
     1021 gcgtaaatct ctggctacaa aaaaggcaac ggcactatca ttttttaaca atgaaacaac
     1081 aaattaacct tgtttcattt tctccatgat tttaccagga agattacgta gaaagggtat
     1141 cctgcaagcc tgtcctggac tatccttgtc acgtcagaca cgcgtgtgtc gttgtgcgct
     1201 ttttttgggt gaaaggagta agaaaatggc gacaggaaag tcctgctctc gctggtttgc
     1261 gcctcttgcg gcgttattaa tggtagttag cctgagtggg tgttttgata aagaaggcga
     1321 tcagcgtaaa gcgtttatcg acttcctgca aaatacagtg atgcgtagcg gtgaacgtct
     1381 accaaccctg actgccgatc agaaaaaaca gtttggtcct tttgtctctg attacgcgat
     1441 tctgtatggt tattctcagc aggtgaatca ggcgatggat tccggtctgc gcccggtcgt
     1501 agacagcgtt aacgcaattc gcgtaccgca ggattacgtt acgcaaagcg gtccgctgcg
     1561 tgaaatgaat ggttcgctgg gtgtgctggc gcaacaactg caaaatgcga agttgcaggc
     1621 cgatgctgcg cactctgcgc taaaacagag cgatgacctg aaaccggtat ttgatcaggc
     1681 attcaccaaa gtggtaacga cgccagctga tgcattgcaa ccgttgattc cggcggcaca
     1741 aactttcacg caacaactgg tgatggttgg agactacatt gctcagcaag gtactcaggt
     1801 gagctttgtc gcaaatggca tccagttccc gacttcacag caggcaagtg aatataacaa
     1861 actgattgcg ccattaccgg cacaacatca ggcgtttaat caggcctgga ctacagcagt
     1921 tactgcaacc caataaagag taaaagcccg agcgattatc gggctttact tcacgcagtc
     1981 gcagcttagt ccgcgacgtg aggattcaca cagttcttct caacctttcc ttgtaacgca
     2041 tcaatcaaat tatccacggc acaggcggcc atgccataac gcgtctcatg ggtggcagat
     2101 ccaatatgcg gtactgcgac gacgttggcc attgagagca acggcgaatc tacggacagt
     2161 ggctcttgtt cgaagacatc cagcccggcg gcgtgaattt cgcctttctg caatgctgcg
     2221 atcagtgcat tttcgtcaac caccgggcca cgtccggcat taatgaaaat ggcggaggat
     2281 ttcattttgg cgaattgttc tgcgccaaac agatgatgcg tctcatcagt taacggcagg
     2341 atcaggcaaa cgaaatctga ctcttgtaac agagtatcca aatcgcagta gcgggcgttg
     2401 aagcgttctt ctgcttcttt atggtggcgg cgcgcgttat agaggatggg catgttgaag
     2461 ccaaagtgca cacgttgtgc cagcgccatg ccgatccgtc ccatcccgac aatgcccagt
     2521 gttttatggt gaacgtcagt gccgtaccag tccgggccta tgctcgcggt ccattcgcct
     2581 gcttttaccc gttctgctac ctccacaacc cgacgagcgg tagacaacac cagcgccatc
     2641 agcgtatcgg cgacggtttc tgttaatacg gttggcgtgt gcatcagcag aatttttcgg
     2701 gcggtaagcg catcgacatc aaaattgtca tagccgacgg agatcgttga tgtggcacgc
     2761 agtttcggca ttttttccag caatgcggca tctacattct cgtttgaacc cagtaaacct
     2821 tcagcttctg caaaaattgc tgcattttga acgacggttt gtgggctgag gtttgccacc
     2881 tggtgaacgg tgaaatgcgc ttgcaggcgt tgcagtaaat catcaggtaa ggctttgtag
     2941 aggataacgg acggcttcat gcttctctcc attcactgat aaggcgatca ccttagcctg
     3001 aaatatgatg aaggaaaact gaagaatgcc aggtgatttc tgcatcacct ggcatgaaag
     3061 ggattacagc gggcttaagg taatttctac acggcggttt tgcgccttac cttctgcggt
     3121 gctgttgctg gcgattgggt tagccgggcc aaggccctga gtacggatgc ggctggcgtc
     3181 cacgccctgg gtgatcaacg cgctggcaac ggaatccgca cgctgctggg agagacgcat
     3241 gttcaggtcg tgaccccccg tgctgtcggt ataaccaatc acgttaaccg ccgttttcgg
     3301 atactctttc agcaccattg ccacgccggt cagggtgtta gcgcctgccg gtttcagggg
     3361 agcgctgctg ctgtcgaagg tcacattgtt cggcatattg aggataatgt tatccccgct
     3421 gcgggttacg ctaacaccag tgccgcgcat tttgtcacgc agcttcgctt cctgcacatc
     3481 catgtaataa cccacgccgc cgcccagagc tgcgcctgct gctgcgccaa tcagcgcgcc
     3541 tttaccgcga tctttcttcg aagaagagag cgcaccaata cccgcgccca cgagagagcc
     3601 cagacctgcg ccgatagcag atttgcctgc ttcgcgttcg ccggtgtaag ggttagttgt
     3661 gcagccagat accgccagag cgccactcac tacggcggca agtaagataa acacgtttct
     3721 tcattgttaa tccttaataa cctttttatt ctttgtcacg ggttccgtgg cgggagatta
     3781 tgccgcgtga acatgaagat tattcctggg aatactcgga aatttgtaag taatatttaa
     3841 ctgctcaata catctaacct ttcaggagtc ttcggtttgg ccaactcatc ctcacgatat
     3901 tccgttctga ctgccgccca ctgggggccc atgctggttg aaaccgacgg cgaaaccgtg
     3961 tttagctcgc gtggcgcgtt agccacagga atggaaaact ccttgcagag cgcggttcgc
     4021 gaccaggttc acagcaatac gcgggtacga tttccaatgg tgcgcaaagg ctttctcgcg
     4081 tcaccggaaa acccgcaagg cattcgtggg caggatgaat ttgttcgcgt gagttgggat
     4141 gaggcgctgg caggcggtta taccgggcat ctgggggatt attcgaccgg tgcggcacag
     4201 gcgatcatgc cgtatgtcgt aggtggcagc gaagtttatc aacagcaaac cagttggccg
     4261 ctggtgctgg aacatagcga tgtcgtggtg ttgtggagtg ctaacccact caatacgctg
     4321 aaaattgcgt ggaatgcatc cgatgagcag gggctttctt acttttccgc actgcgtgac
     4381 agtgggaaaa agctgatctg cattgatcca atgcgatcgg aaaccgtcga tttctttggc
     4441 gataaaatgg agtgggtggc accgcacatg ggcaccgatg ttgcgctgat gctggggatc
     4501 gcccatacgc tggtggaaaa tggttggcac gacgaagcgt ttctggcgcg ttgcaccaca
     4561 ggttatgccg tcttcgcctc ttatttgctg ggcgagagtg acggaatagc gaaaaacgcc
     4621 gaatgggcgg cagagatttg tggtgttggc gcagcgaaaa tccgcgagct ggcggctatt
     4681 ttccaccaaa ataccaccat gctgatggca ggctggggaa tgcagcgcca acagtttggt
     4741 gagcaaaaac actggatgat cgtcacgctg gcagcaatgt tggggcaaat cggcacaccc
     4801 ggcggcggtt ttggtctttc ttaccatttt gccaatggtg gtgaccccac gcggcgttct
     4861 gcggtgctct cttccatgca gggcagcttg ccgggtggct gcgatgcggt ggataaaatc
     4921 cctgttgccc gcattgttga agcactggaa aaccctggtg gcgcatatca acacaacggt
     4981 atgaaccgac atttcccgga tattcgtttt atctggtggg cgggcggtgc caactttact
     5041 catcatcagg ataccaatcg tctgatccgt gcctggcaaa aaccggagct ggtggtgatc
     5101 tctgaatgct tctggacggc ggcggcgaaa cacgcggata ttgttctgcc tgcgactacc
     5161 tcttttgagc gtaatgatct caccatgacc ggtgattaca gtaatcagca tctggtgccg
     5221 atgaagcaag tggtgccgcc acgctatgaa gcgcgtaatg attttgatgt ttttgccgag
     5281 ttgagtgaac gttgggagaa gggcggctat gcgcggttta cggaaggaaa aagtcagctg
     5341 caatggctgg aaacgtttta taacgttgcc cggcagcgcg gggcaagcca gcaggttgaa
     5401 ttgccgccat ttgctgagtt ctggcaagcc aaccagttaa ttgagatgcc ggaaaacccg
     5461 gacagcgagc ggtttattcg cttcgctgat ttttgccgcg atccgctggc gcatccgtta
     5521 aaaaccgcca gcggcaagat tgaaatcttc tcacagcgta ttgccgatta cggttacccg
     5581 gattgccctg ggcatccaat gtggctggag ccggacgaat ggcagggcaa tgccgaacca
     5641 gaacagttgc aggtactttc tgcccatccg gcgcaccgcc tgcacagcca gctgaattac
     5701 agttctctgc gcgaattgta cgcggtagca aaccgtgagc ctgtcaccat tcatcctgac
     5761 gatgctcagg cgcgcggcat aaccgaaggg gatatggtgc gggtgtggaa ctcccgtggg
     5821 cagatccttg ccggagcggt cattagcgag ggaattaaac ctggcgtgat ttgcattcac
     5881 gaaggggcat ggccggatct ggatttaacc gctgacggta tttgtaaaaa cggcgcagtg
     5941 aacgtgctga ccaaagatct ccccagctcg cggctgggga atggctgtgc gggtaatacg
     6001 acgctggcat ggctggaaaa atacaacggt ccggaactga cacttacagc gtttgaacca
     6061 ccggccagct cataatccag gtgggtagtt gggtttcatc ctgccatgcg caatcgacaa
     6121 tgtgaaaacc ctgcgcacgg taaaaatcta tcgccggttg atttttttga taaacctcca
     6181 gcatcagatg gggatagcgc tgctgcacat actgcatcag cgccttacca ataccgcgtc
     6241 tgacggcctt cggtgcgaca aacattgctg ccagaaatcg gccttccata atgctgacaa
     6301 aaccgagaag cttaccgtcc tcttcccaga cccagttttg cgcgttggca agataggcat
     6361 cccgcaccag cggaatgcag tcacgccagt aattcgcttt tataaaggga tgcccccagg
     6421 ttgtactttc cagccacagt tcgaggatcg cggggagttc tgaacgttgc gcttcccgaa
     6481 tcatggttta tttcccagat agcaacagca gccaaccaca tgatcattca ccagcccaca
     6541 tgcctgcata aaggagtaac agattgtggt gccgacaaac ttaaaaccac gttttttcag
     6601 tgccttagat agggcgtcgg aggcggacgt agatgtggga atttcgctca acgttgtggc
     6661 ttgtgtcacc tgtggctgat gatttacaaa cgaccagaca aagtcgggaa acggttcgcc
     6721 gttctgttcc atttgcaggt acgcccgcgc attaccaata attgcctgaa ttttccctcg
     6781 atggcggata atcccggcgt cctgtaccag tctttcgaca tcctcttcct gcattgctgc
     6841 gaccttcacc ggatcgaact gatgaaagta ggcgcgatag ttttcgcgtt ttttgaggac
     6901 ggtgatccac gataacccag cctgctgccc ttcaaagcag atcatttcga acagtttttt
     6961 actgtcagtt tcaggcacgc cccactcatt atcatggtag gcaatataaa gcgggccctg
     7021 actcacccag ccgcaacgtt ccatactttc cctcgctatg tacccgatcg taaaaatttc
     7081 agccgctttt cttgacgcat cggtgaaaaa gtcaatattt aatacagggc tactatgccc
     7141 gatatccttc atacaatgac aataatattg ccgaattcgg ctcttccctg gagtcgtgtt
     7201 aatgatcata aaaaaaagcg gtggtcgctg gcagctaagc ctgctggcga gcgtggtaat
     7261 cagtgccttt tttctcaaca cagcttacgc ctggcaacaa gaatatatcg ttgatacgca
     7321 acccggacat tccacagagc gttacacctg ggatagtgat catcaacctg attacaacga
     7381 tattttgtcg caacgtattc aaagtagcca aagggcgctg ggactggaag tcaatctggc
     7441 ggaagaaact cctgtggatg tgaccagcag tatgagtatg ggctggaatt ttcctttata
     7501 tgaacaggtt acaaccggcc cggtcgcggc attacattac gatggcacaa ccacctcgat
     7561 gtataacgag tttggcgaca gtactaccac gctgaccgat ccgttatggc atgccagcgt
     7621 gagtagctta ggctggcgtg ttgactcccg gcttggcgat ctccgaccct gggcgcaaat
     7681 cagctataac cagcaatttg gcgagaatat ctggaaggcg caatcaggcc tgagccggat
     7741 gacggcgaca aaccagaacg gcaactggct ggatgtcacc gtaggcgctg atatgttgct
     7801 caatcaaaat attgccgcct atgctgcgct aactcaggca gaaaatacca ctaataatag
     7861 cgactatctg tatacgatgg gggttagcgc cagattttaa cgtaacagtc acaattgaaa
     7921 ccattgaata acaacagttg tggcgatagt gggtgctaac ttaccaaata ataaatttgg
     7981 tgaataattg tcgcgtcatt cattcctgaa ctaaggcatt tcattccgtt ctgatggcat
     8041 ttcatgccgt ttttccccaa gcataaaatg cacttcgtta tggttgtcgg cagagatttt
     8101 tcctttttat tactgcagga atactgccat gacaccttca aattatcagc gtacccgctg
     8161 gctgacactc atcggtacta tcattaccca gtttgcgctg gggtcggttt atacccggag
     8221 cctgtttaat ggcgcgcttt ctgccaagct ggatgcgccg gtaagccagg tcgctttctc
     8281 tttcggcttg ttaagtctgg ggctggcaat ttcgtcttct gttgcgggca aattgcagga
     8341 acgttttggc gttaaacgcg tcaccgtggc ttccggcatt ttgctgggat taggcttctt
     8401 cctgaccgcg cattccaaca acctgatgat gctgtggtta agcgccggtg tgctggtcgg
     8461 tctggcggat ggcgcgggtt acctgctgac gctctctaac tgcgtgaagt ggttcccgga
     8521 gcgtaaaggt ctgatctccg cgttcgctat cggttcttat ggtctgggta gcctgggttt
     8581 caaatttatc gacacgcagc tgctcgaaac ggtgggtctg gaaaaaacct ttgtgatttg
     8641 gggagcgatt gcgctggtga tgattgtttt cggcgcaacg ttaatgaaag acgcaccaaa
     8701 acaggaagtg aaaaccagca atggtgtggt ggagaaagat tacacgctgg cagagtcgat
     8761 gcgtaaaccg cagtactgga tgttagcggt aatgttcctg actgcctgca tgagcggcct
     8821 gtacgtgatt ggggtagcga aagatatcgc ccaaagtctg gcacaccttg atgtggtttc
     8881 cgcagccaat gcagtaacgg ttatttccat cgctaacctt tcaggtcgtc tggtgctggg
     8941 cattctgtct gacaaaattg cccgtatccg tgttatcacc attggtcagg tgatttcgct
     9001 ggtgggtatg gcggccctgc tgtttgcacc attaaatgca gtgacgttct ttgcagcgat
     9061 tgcctgcgtg gcatttaact ttggcggcac tattaccgtc tttccatcac tggtcagtga
     9121 gttcttcggc ctcaataacc tggcgaaaaa ctacggtgtg atttacctcg gtttcggtat
     9181 cggtagcatt tttggttcga ttatcgcctc actgtttggc ggtttctatg tgaccttcta
     9241 cgtgattttc gccctgctga ttctgtcatt ggcgctttct acgacgattc gtcagccaga
     9301 gcagaaaatg ttgcgtgagg cgcatggctc cctttaatgc tcagccgcga gctactgggc
     9361 ttcagcttcc gtgtgcatca aacgcatcaa cgaaatgtat aaacaatgac tcgtaaaatt
     9421 tgggccagtt aactggccct ttttactgct ttttgtaacg ctaaagtctc ttttcaaact
     9481 tgcatttttg taaatttgtg cttcatgcac actctttccc cacacttttt cgctttgctg
     9541 tggtctactt attcgcgcgt gtagatttta cttatctgac tacctccgca cttttcccct
     9601 gccgggcctg aaaagccgct aagcagggtg ttatcacctg tttgtccagg gtttgtttgc
     9661 atgagataca tcaaatcgat tacacagcag aagctgagct ttttgcttgc aatctatatt
     9721 ggccttttta tgaatggcgc ggttttttat cgccgcttcg gcagctatgc gcacgatttt
     9781 accgtctgga aaggcgtttc tgctgttgtt gaactggccg ccaccgtact ggtgaccttc
     9841 tttttactac gtcttctatc gctgtttggc cgccgcagct ggcgtattct ggcgtcgctg
     9901 gtggtgctct tttccgcagg tgccagctat tacatgacct tccttaatgt ggtcattggt
     9961 tatggcatca tcgcttccgt catgaccacc gatatcgacc tgtcaaaaga agttgttggt
    10021 ctgaacttta ttctctggtt aatcgccgtt agtgcattgc ctcttatcct tatctggaat
    10081 aaccgctgtc gctacacctt gctccgacaa ctgcgaaccc cagggcagcg tattcgcagc
    10141 ctggtggtcg tcgtactggc gggtattatg gtttgggcac cgattcgttt gctggatatc
    10201 cagcagaaga aagtggagag ggcgaccggc gttgatttgc cgagttatgg cggtgtcgta
    10261 gcgaactctt atctgccatc aaactggctt tctgcgttgg ggctgtatgc ctgggcgcgg
    10321 gtcgatgaat cttccgataa taattcattg cttaatccgg cgaagaagtt tacataccag
    10381 gctccacaaa acgttgatga tacttatgtc gtgtttatca tcggtgaaac cacgcgttgg
    10441 gaccatatgg gtattttcgg ctatgagcgt aataccacgc cgaaactggc ccaggagaaa
    10501 aatctggcgg cgttccgtgg ttactcctgt gataccgcaa ccaaactctc actgcgttgc
    10561 atgtttgtac gtcagggggg cgcggaagat aatccgcagc gcacattaaa agaacagaac
    10621 attttcgcgg ttctgaagca gttaggattc agttctgacc tctacgctat gcagagcgaa
    10681 atgtggttct acagcaacac gatggcagac aacattgctt atcgtgagca gattggtgcg
    10741 gagccacgta atcgtggcaa gccggtagat gatatgttgc tggtagacga aatgcagcaa
    10801 tcgctagggc gcaacccgga tggtaagcat ctgatcattc tgcataccaa aggttcgcat
    10861 tttaactaca cccagcgtta tccgcgtagc ttcgcgcagt ggaagccgga atgtattggt
    10921 gttgatagcg gctgtaccaa agcgcagatg atcaactcct atgacaactc ggtgacctat
    10981 gtggatcact ttatctccag cgtgattgat caggttcgcg ataagaaagc gattgtgttc
    11041 tacgcagctg accacggtga gtcaattaat gaacgcgagc acctgcacgg cacgccgcgt
    11101 gaactggcac cgccggagca gttccgcgta ccgatgatgg tctggatgtc agataaatat
    11161 ctggaaaatc cggccaatgc gcaggcgttt gcgcagctga aaaaagaagc cgacatgaaa
    11221 gtgccacgcc gtcacgtaga gctgtacgac accatcatgg gttgtcttgg ctatacttca
    11281 ccggatggtg gaattaacga aaacaacaac tggtgtcaca tcccgcagac aaaagtggca
    11341 gcggctaact aagcggcctg ctgactttct cgccgatcaa aaggcatttt gctattaagg
    11401 gattgacgag ggcgtatctg cgcagtaaga tgcgccccgc attcggtgat tggcgcagcc
    11461 tggtagcgca cttcgttcgg gacgaagggg tcggaggttc gaatcctcta tcaccgacca
    11521 aattcgaaaa gcctgctcaa cgagcaggtt tttttgcatt tgtagctcat gaggatgaga
    11581 acctccgggg gcagggaggc tcgactcgac gtaggcctga taagacgcgc cagcgtcgca
    11641 tcaggcaacg gcaccgcact tattgtcgga tgcggcgcga ccgccttatt cgaccaacac
    11701 accacacatt ttcctgccaa ctttgcgccc catatctcac tatccattaa cggatttgtg
    11761 acagaaacca ttgtcttttt ttatatttgc ttaaatcttt tttgcgttac ttatggctgg
    11821 cgctggcatt ttccgctgtg cgttttagcg ttcatggtat taatcgctca gatatccacc
    11881 ttttcctcag atttttttca ttaaatgcat aaaacttaat caccaaatgt tgctaaatat
    11941 taagctgatt gttctggtta tgttggagta gcacaaattt ctctgctgca aatagctgtt
    12001 tgaagttttt ttaatctttg tttgtggatt ctcaccgttg gtgtaaatcc cggttggctg
    12061 tattgacgtt ttcacattct gttgacagat tgtaggtcac gaggggcatt ttatggagga
    12121 tccgcactgt tacactgatg ttaattagta cggcatcccc acctcataac gttgacccga
    12181 ccgggcaaaa aacaaaaaag gtcaggcagc gacaacccac tgcaaagggt taaaacaaca
    12241 aacatcacaa ttggagcaga ataatgcgta tttccttgaa aaagtcaggg atgctgaagc
    12301 ttggtctcag cctggtggct atgaccgtcg cagcaagtgt tcaggctaaa actctggttt
    12361 attgctcaga aggatctccg gaagggttta acccgcagct gtttacctcc ggcaccacct
    12421 atgacgcctc ttccgtcccg ctttataacc gcctggttga atttaaaatc ggcaccaccg
    12481 aagtgatccc gggcctcgct gaaaagtggg aagtcagcga agacggtaaa acctatacct
    12541 tccatctgcg taaaggtgtg aagtggcacg acaataaaga attcaaaccg acgcgcgaaa
    12601 tgaacgccga tgacgtggtg ttctcgttcg atcgtcagaa aaacgcgcaa aacccgtacc
    12661 ataaagtttc tggcggcagc tacgaatact tcgaaggtat gggcttgccg gagctgatca
    12721 gcgaagtgaa aaaggtggac gataacaccg ttcagtttgt gctgactcga ccggaagcgc
    12781 cgttcctcgc tgacctggca atggacttcg cctctattct gtcaaaagaa tatgctgacg
    12841 cgatgatgaa agccggtaca ccggaaaaac tggacctcaa cccaatcgga accggtccgt
    12901 tccagttaca gcagtaccaa aaagattccc gtatccgcta caaagcgttt gatggctact
    12961 ggggcaccaa accgcagatc gatacgctgg tcttctctat tacccctgat gcttccgtgc
    13021 gttacgcgaa attgcagaag aatgaatgcc aggtgatgcc gtacccgaac ccggcagaca
    13081 tcgctcgcat gaagcaggat aaatccatca acctgatgga aatgccgggg ctgaacgtcg
    13141 gctacctctc gtataacgtg cagaaaaaac cgctggatga cgtgaaagtt cgccaagcac
    13201 taacctacgc ggtgaacaaa gacgcgatca tcaaagcggt ttatcagggc gcgggtgtat
    13261 cagcgaaaaa cctgatcccg ccaaccatgt ggggctataa cgacgacgtt caggattaca
    13321 cctacgatcc tgaaaaagcg aaagccttgc tgaaagaagc gggtctggaa aaaggtttct
    13381 ccatcgacct gtgggcgatg ccggtacaac gtccgtataa cccgaacgct cgccgcatgg
    13441 cggagatgat tcaggcagac tgggcgaaag tgggcgtgca ggccaaaatc gtcacctacg
    13501 aatggggtga gtacctcaag cgtgcgaaag atggcgaaca tcagacggta atgatgggct
    13561 ggactggcga taacggggac ccggataact tcttcgccac cctgttcagc tgcgccgcct
    13621 ctgaacaagg ttctaactac tcaaaatggt gctacaaacc gtttgaagat ctgattcaac
    13681 cggcgcgtgc taccgacgac cataacaaac gcgttgaact gtacaagcaa gcgcaggtgg
    13741 tgatgcacga tcaggctccg gcactgatca tcgctcactc caccgtgttt gaaccggtac
    13801 gcaaagaagt caaaggctat gtggttgatc cattaggcaa acatcacttc gaaaacgtct
    13861 ctatcgaata attaaaagcc atacaagact gatggcaaag gcaaaactgc ctgatgtgct
    13921 ccgcttatca ggcctacgaa aattctgcaa tgtattgaat ttgcacgatt ttgtaggccg
    13981 gataaggcgt taacgccgca tccggcataa acaaagcgca ctttgtcaac aatctgtata
    14041 cccggtggcg ctgtgcctct gcgtgcagcg ccatccggca gcaatacttc attctctgtc
    14101 cgacaagggc gggaattgat ttgtgagcaa tacagacacg cagttccagg ctgcgagtca
    14161 ctatagagaa tccgggttat gttgcagttt attctccgac gtttgggact cgtcatcccc
    14221 acgtttatcg gtattaccct tctcacattt gcctttgtcc acatgatccc gggcgatccg
    14281 gtgatgatca tggcaggcga acgtgggatc tcccctgagc gtcacgtgca gctgctggct
    14341 gaactcggct tagataaacc gatgtggcag cagtatctcc attacatttg gggcgtaatg
    14401 cacggcgatc taggcatttc aatgaaaagc cgaattccag tatgggaaga gttcgtgccg
    14461 cgctttcagg ccacgctgga acttggcgtc tgcgcgatga tttttgctac ggcagtcggt
    14521 attccggttg gtgtgctggc tgcggttaaa cgcggttcca ttttcgatca cactgcagtt
    14581 ggcctggcgc tgaccggcta ctcgatgccg atcttctggt ggggcatgat gctgatcatg
    14641 ctggtttcgg tgcactggaa cctgacgccc gtctccggtc gcgtgagcga tatggtgttc
    14701 ctcgatgact ccaatccgtt aaccggtttt atgctaatcg acaccgccat ctggggtgaa
    14761 gacggcaact ttatcgatgc cgtcgcccat atgatcttgc ccgccattgt gctgggtact
    14821 attccgctag cggtcattgt gcgtatgaca cgctcctcga tgctggaagt gctgggcgag
    14881 gattacatcc gcaccgcgcg cgccaaaggg ctaacccgca tgcgggtgat tatcgtccat
    14941 gcgctgcgta acgcgatgct gccggtagtg accgttatcg gcctccaggt gggaacattg
    15001 ctggcggggg cgattctgac cgaaaccatc ttctcgtggc ccggtctggg gcgctggttg
    15061 attgacgcac tgcaacgccg cgactatccg gtagtgcagg gcggcgtatt gctggtggcg
    15121 acgatgatta tcctcgtcaa cttgctggtc gatctgctgt acggcgtggt gaacccgcgt
    15181 attcgtcata agaagtaagg ggacatcatg tcacaggtta ctgaaaataa agtgattagc
    15241 gcaccggtgc cgatgacccc gttacaggag ttctggcact attttaaacg caacaaaggc
    15301 gcggtcgtcg ggctggttta cgtcgccatc gtgctgttca tcgcgatctt tgccaactgg
    15361 attgcgcctt ataacccggc ggatcagttc cgcgatgcgc tgctcgcccc gccagcctgg
    15421 caggaaggcg gcagtatggc acacttgctg ggcaccgatg acgtcggtcg tgatgtgctg
    15481 tcgcggctga tgtacggcgc gcgtttatcg ctgctggttg gctgtctggt ggtggtgttg
    15541 tcgctgatta tgggcgttat tctcggcctg atcgccggtt acttcggcgg cctggttgat
    15601 aacattatca tgcgcgtggt cgatatcatg ctggcgctgc caagtctgct gttggcactg
    15661 gtgctggtgg cgattttcgg cccgtcgatt ggtaacgccg cgctggcgct gaccttcgtt
    15721 gccttgccgc actatgtgcg cttaacccgc gccgccgtgc tggtggaggt gaatcgcgat
    15781 tacgtcaccg cctctcgcgt ggcaggtgcc ggggcgatgc gccagatgtt tattaacatc
    15841 ttcccgaact gccttgcgcc gctgattgtt caggcgtcgc tcggtttctc taacgccatt
    15901 ctcgatatgg ctgctcttgg tttcctcggc atgggggcac agccgccaac gcctgagtgg
    15961 ggcaccatgc tctccgacgt gttgcagttc gcgcaaagcg cctggtgggt cgtgaccttc
    16021 cctggtctgg cgatcctgtt gacggtgctg gcatttaacc tgatgggtga cggtctgcgt
    16081 gacgcgctcg atcccaaact aaagcagtaa gaggttcgag atggcgttat taaatgtaga
    16141 taaattatcg gtgcatttcg gcgacgaaag cgcaccgttc cgcgccgtag accgcatcag
    16201 ctacagcgta aaacagggcg aagtggtcgg gattgtgggt gagtccggct ccggtaagtc
    16261 ggtcagttca ctggcgatta tggggctgat tgattatccg ggccgcgtaa tggcagaaaa
    16321 actggagttt aacggccagg atttgcagcg tatctcagaa aaagagcgcc gcaacctggt
    16381 gggtgccgaa gtggcgatga tcttccagga cccgatgacc agccttaacc cgtgctacac
    16441 cgtgggtttc cagattatgg aagcgattaa agtgcatcag ggcggcaaca aaagtacccg
    16501 ccgtcagcgg gcgattgacc tgctgaatca ggtcggtatt cccgatccgg cttcgcgtct
    16561 ggatgtttac ccgcatcagc tttccggcgg catgagccag cgcgtgatga tcgccatggc
    16621 gattgcctgt cggccaaaac tgctgattgc cgatgaaccg accaccgcgc tggacgtgac
    16681 cattcaggcg caaatcatcg aactactgct ggagctacag cagaaagaga acatggcgct
    16741 ggtgttaatt acccatgacc tggcgctggt ggcggaagct gcgcataaaa tcattgtgat
    16801 gtatgcaggt caggtagtag aaacgggcga tgcacacgcc atcttccatg cgccgcgtca
    16861 cccgtatact caggcattgc tgcgtgcgct gccagaattt gctcaggaca aagaacgtct
    16921 ggcgtcgttg ccaggtgttg ttcccggcaa gtatgaccgc ccgaacggct gcctgcttaa
    16981 cccgcgctgc ccctatgcca ctgacagatg tcgcgctgaa gaaccggcgc tgaatatgct
    17041 cgctgatggg cgtcagtcca aatgccatta cccacttgat gatgccggga ggcccacact
    17101 atgagtacgc aagaggccac ctcgcaacaa ccgctgttgc aggctatcga cctgaaaaaa
    17161 cattatccgg tgaagaaagg catgttcgtg ccggaacgtc tggtgaaggc gctggacggc
    17221 gtttcgttta accttgaacg tggcaaaacg ctggcagtag tgggcgaatc tggctgcggt
    17281 aaatcgaccc tcggtcggtt gctgacgatg atcgaaacgc ccaccggcgg cgagctgtat
    17341 tatcaggggc aggatctgct caagcacgat ccgcaggcgc agaagctgcg tcggcagaaa
    17401 atccagatcg tcttccagaa tccctacggt tcgctgaatc cgcgtaagaa agttgggcaa
    17461 attctcgaag agccgctgct tattaacacc agcttaagca aagaacagcg tcgggaaaaa
    17521 gccctgtcga tgatggcaaa agtcggcctg aaaaccgagc actatgaccg ctatccgcac
    17581 atgttttccg gcggacaacg tcagcgtatc gctattgccc gtggtctgat gctcgacccg
    17641 gatgtggtga ttgccgatga gccggtttct gcgctggacg tctcagtgcg tgcgcaggtg
    17701 ctgaatctga tgatggattt gcagcaggag ttggggcttt cttatgtctt tatctcccac
    17761 gacctgtcgg ttgtggagca cattgctgat gaagtgatgg tgatgtacct aggccgctgc
    17821 gtggagaaag gaacgaagga ccaaatcttc aataacccgc gccatccgta cacccaggcg
    17881 ctgctctccg cgacgccacg cctgaacccg gacgatcgtc gtgaacgcat caaactcacc
    17941 ggtgaactgc caagcccgct gaatcctccg ccgggttgcg ccttcaacgc ccgctgtcgt
    18001 cggcgcttcg gcccctgcac ccagttgcag ccgcagctaa aagactacgg aggtcaactg
    18061 gtagcttgtt ttgctgttga tcaggatgaa aatccgcagc gttaactcac aaaaccgggg
    18121 ccatgcgctc cggtttactc aatcagcttc aacagtggcg acaagcatag caaaatgccg
    18181 taaagcagaa tcagccaggc tttaaatccg cgaagttttt ccagttgtgc gactttatag
    18241 atcaggaaaa acggaatgag gcacgacaca atgccatata acgggcttcc caactgaaag
    18301 aacaccagca ctgatacacg aaacgaaacc caaatcgtca acgtaatgac gataaaagcg
    18361 cagatcgcca gagtcagcat gcgtgagtta attttcttgg tatcaataat tcggcttaac
    18421 agattgagaa taatgccttt aatagcctcg tggaaaccga gataaatgcc aaagaatgcg
    18481 gtcagtacgg caaagatatt cagcaccgta gaggtgatat gaatgatatg tccagggatc
    18541 acctgcgcgg ccagtgccag tgccgagata ttttgttcaa aggcagaaac ggcttcttcg
    18601 tggctaattg agaaggtaaa cgaaaaggca aaaaacagga tcaccgcgat gagcgtgata
    18661 taactaattc ggtgggtacg cagcgcgagc cgggttgcca gtactttatc tgcttcccgt
    18721 ttacgatagg caatattcat tgggttaagt acctgaataa ataccgcaga aaagaagcaa
    18781 aatggaatgg taagcaggac atcgcggaaa aagacggagg cttgcgggaa ggcggttata
    18841 ttggcgaaat tccagtgcgg gatcatcgca aaaccgaaca cgacaataat ccctactttg
    18901 accaccacca ttggcccaga aatcttaaac agtaatcgtt caccaccaga cgcaatcgct
    18961 atcagtacgg cgaaaatagc gactttataa agtagagatt gtgaaagatc ggcatcggtt
    19021 aaaccgaagg ttttcaggta cgaggcgctg tcgaaaacca cggagagaga gtagataaaa
    19081 atcccgtgga taatcatcaa aaagtagata accccgagga aaattcccca gttcttcccc
    19141 aggtaatgac tgataatatc ggtgtagtca ttacaggaat cgctttcaga aagggttttt
    19201 aaataaatgt cctgcactac ccaggtgaca ggataagcaa tgatcgctgc ggtaataaat
    19261 acccaaattc ccttcaagcc aatttgtact ggcatcagca cggttccggc accaatcgcc
    19321 atgcctatgc ataataaaac ccagccgaag tcgtagcgtg taaacggcaa tttctgattg
    19381 tgtttcgata gtgtgttgtg ctgcataaag tcctgtccag aaagaagtcg gcctcgcaaa
    19441 aatgaataaa cccagtttct cttgcgcaac aggtggagaa gtcgaatacg cagtatgtaa
    19501 aaaatgaggc aacagattca ctggggaggt cgcgaggagg cgcaattcta caaaattgcg
    19561 catttcacta ccagtctgga gtcagcccgg atatgaactg caactattgc aaataaagaa
    19621 attaagctgg aaggcatcgc gcaatgagga agattgccgg gtgctgacag taaaaagcaa
    19681 tcgtagaatc agcgtcaagc cggaaggatt ccggtgaggc gcaatgttgc gggggcttaa
    19741 tccctggtgg cattggttgc tggagagaga aaacccccgc acgttgcagg tatgtacctg
    19801 acaacataac gggggctaat cttgaaccta gaccactcaa gaatagccgc gaaacgttgt
    19861 cattacaaca caggcggcta tatgacgctc gcagagctgg gcatggcctt ctggcatgat
    19921 ttagcggctc cggtcattgc tggcattctt gccagtatga tcgtgaactg gctgaacaag
    19981 cggaagtagc gtgtcatg
//
LOCUS       DAZPAB010000062        19748 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZPAB010000062 DAZPAB010000000
VERSION     DAZPAB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.062"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            258..1355
                     /gene="mltA"
                     /locus_tag="ACHUPG_001957"
     CDS             258..1355
                     /gene="mltA"
                     /locus_tag="ACHUPG_001957"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR0861586.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1593..2399
                     /gene="tcdA"
                     /locus_tag="ACHUPG_001958"
     CDS             1593..2399
                     /gene="tcdA"
                     /locus_tag="ACHUPG_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR0861587.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2450..2893)
                     /gene="csdE"
                     /locus_tag="ACHUPG_001959"
     CDS             complement(2450..2893)
                     /gene="csdE"
                     /locus_tag="ACHUPG_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR0861588.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2893..4098)
                     /gene="csdA"
                     /locus_tag="ACHUPG_001960"
     CDS             complement(2893..4098)
                     /gene="csdA"
                     /locus_tag="ACHUPG_001960"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR0861589.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4290..4517
                     /locus_tag="ACHUPG_001961"
     CDS             4290..4517
                     /locus_tag="ACHUPG_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR0861590.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            4868..5785
                     /gene="gcvA"
                     /locus_tag="ACHUPG_001962"
     CDS             4868..5785
                     /gene="gcvA"
                     /locus_tag="ACHUPG_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR0861591.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5804..6199
                     /locus_tag="ACHUPG_001963"
     CDS             5804..6199
                     /locus_tag="ACHUPG_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR0861592.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6192..7292
                     /gene="rlmM"
                     /locus_tag="ACHUPG_001964"
     CDS             6192..7292
                     /gene="rlmM"
                     /locus_tag="ACHUPG_001964"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR0861593.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7336..8067)
                     /gene="fucR"
                     /locus_tag="ACHUPG_001965"
     CDS             complement(7336..8067)
                     /gene="fucR"
                     /locus_tag="ACHUPG_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR0861594.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8125..8547)
                     /gene="fucU"
                     /locus_tag="ACHUPG_001966"
     CDS             complement(8125..8547)
                     /gene="fucU"
                     /locus_tag="ACHUPG_001966"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR0861595.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8549..9968)
                     /gene="fucK"
                     /locus_tag="ACHUPG_001967"
                     /pseudo
     CDS             complement(8549..9968)
                     /gene="fucK"
                     /locus_tag="ACHUPG_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(10077..11852)
                     /gene="fucI"
                     /locus_tag="ACHUPG_001968"
     CDS             complement(10077..11852)
                     /gene="fucI"
                     /locus_tag="ACHUPG_001968"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFR0861596.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11885..13201)
                     /gene="fucP"
                     /locus_tag="ACHUPG_001969"
     CDS             complement(11885..13201)
                     /gene="fucP"
                     /locus_tag="ACHUPG_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR0861597.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAATPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13748..14395
                     /gene="fucA"
                     /locus_tag="ACHUPG_001970"
     CDS             13748..14395
                     /gene="fucA"
                     /locus_tag="ACHUPG_001970"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR0861598.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14423..15571
                     /gene="fucO"
                     /locus_tag="ACHUPG_001971"
     CDS             14423..15571
                     /gene="fucO"
                     /locus_tag="ACHUPG_001971"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR0861599.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15624..16379)
                     /gene="xni"
                     /locus_tag="ACHUPG_001972"
     CDS             complement(15624..16379)
                     /gene="xni"
                     /locus_tag="ACHUPG_001972"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR0861600.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16490..17857)
                     /gene="sdaB"
                     /locus_tag="ACHUPG_001973"
     CDS             complement(16490..17857)
                     /gene="sdaB"
                     /locus_tag="ACHUPG_001973"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR0861601.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17915..19204)
                     /gene="sdaC"
                     /locus_tag="ACHUPG_001974"
     CDS             complement(17915..19204)
                     /gene="sdaC"
                     /locus_tag="ACHUPG_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR0861602.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4872 a         4841 c         5212 g         4823 t
ORIGIN      
        1 gaccttcggg ttatgagccc gacgagctac caggctgctc caccccgcgt ccgtggatgc
       61 gcactatact ccgctcgcgt tttcatgcaa cctttttttt accccgatca cggatttagc
      121 atgattttga acaaaaacaa accactaaag cctgtttttt gtgcaaaatt tgcgtcaggg
      181 agaggctgct catttcatta aacgcggcgg tttgttatct tcgttgcgcc ttatttttta
      241 acctgaagaa gagaacaatg aaaggacgtt gggtaaagta ccttcttatg ggcacggttg
      301 tggcaatgct tgccgcctgc tcttccaaac caaccgatcg cggacagcaa tataaagacg
      361 ggaaatttac ccagcctttc tctctggtga accagccaga tgccgttggc gcgccgatta
      421 acgccggtga ttttgccgag caaattaacc atatccgtaa ttcgtcaccg cgtctgtatg
      481 gcaaccagag taatgtttat aacgcggtgc aagagtggct gcgcgcaggc ggtgataccc
      541 gcaatatgcg ccagttcggc attgatgcct ggcagatgga aggtgccgac aactatggta
      601 acgtgcagtt tacaggctat tacacgccgg taattcaggc gcgccatacc cgccagggcg
      661 agttccagta tcctatttac cgtatgccgc caaaacgtgg tcgtctgccg tctcgtgcgg
      721 agatctacgc cggggcattg ggcgataaat acatcctcgc ttacagcaac tctctgatgg
      781 ataacttcat tatggatgtg cagggtagtg ggtatatcga ctttggtgat ggcagtccgc
      841 ttaacttttt cagctatgca gggaaaaacg gtcatgccta tcgcagcatt ggtaaggtgc
      901 tgatcgaccg tggcgaagtg aaaaaagaag atatgtcgat gcaggcgatt cgtcattggg
      961 gcgaaacaca cagtgaagcc gaggttcgcg agctgctgga acagaacccg tctttcgtct
     1021 tctttaaacc gcaatctttt gcaccggtga aaggggcaag tgcggtgccg ctggttggcc
     1081 gtgcttctgt tgcctccgat cgttcaatta ttccgccagg tactaccttg ctggcagaag
     1141 tgccgctgct ggataataac ggcaaattta acggtcagta cgaattgcgt ctgatggtgg
     1201 cgttggatgt cgggggcgca atcaaaggcc aacacttcga tatctatcaa gggatcgggc
     1261 cggaagccgg acaccgcgca ggttggtaca accactatgg acgggtttgg gtgctgaaaa
     1321 cggctccggg cgcaggtaac gtctttagcg gctgatgtga tattctgtat gccgttttta
     1381 cggatatgac aggggagggg taacctcaca tcctgattat tgactaagtg cgcgttgttc
     1441 ataccggatg cggcgtgaac gccttatccg gcctacaaat catgcaaatc caaaatattg
     1501 caggagttat gtaggcctga taagcgtagc gcatcaggca tttatgcgtt tagggtgagg
     1561 gataacttcg ccctttttaa ttctgaggtg ctatgtctgt ggtaattagt gatgcatggc
     1621 gtcagcgttt tggtggcaca gcgcgtctgt atggtgaaaa agcgttgcaa ctgtttgctg
     1681 acgcgcatat ttgtgtggtt ggtatcggtg gtgtcggttc ctgggcggcg gaagcgctgg
     1741 cgcgcacggg gattggcgca atcacgctta tcgatatgga tgatgtgtgc gtcaccaata
     1801 ccaatcggca aattcacgct ctgcgcgata acgtcgggct ggcaaaagcg gaagtcatgg
     1861 cggagcgcat tcgccagatt aacccggagt gccgtgtaac ggtggtggat gatttcgtga
     1921 cgccggataa cgtagcgcaa tatatgagtg ccggttattc gtacgtgatt gatgccattg
     1981 atagtgtacg gcccaaagcg gcgctgattg cttattgtcg gcgcaataaa atcccgctgg
     2041 tcacgaccgg tggcgcgggt gggcagattg acccgacgca gattcaggtt accgatctgg
     2101 cgaaaacgat tcaggatccg ctggcggcga agttgcgcga gcgcctgaaa agcgattttg
     2161 gcgtagtgaa aaacagtaaa ggtaagctcg gcgtggattg cgtgttttct actgaagcgc
     2221 tggtgtaccc gcagtcagac ggtacggtgt gtgcgatgaa agccacggca gaagggccga
     2281 agcggatgga ttgtgcatct ggatttggcg cggcaacgat ggtgaccgcc acctttgggt
     2341 ttgttgcggt ttctcatgcg ctgaagaaga tgatggcgaa agcggcgcgt cagggttaaa
     2401 caatcaggtc ggatgagacg cgaacgtctt atccgacccg gttctgtgct taaacctgct
     2461 tcgtcgcagc gataatcgcc tcgcttaacg catttaaccc ctggctgcgt gaggcgctaa
     2521 gctgcgcacg taatcccagc tcatcaaaca atgccagcgg cgactgtgcc tgtaattcgg
     2581 cggcggtttt cccctcaacg gcagtcaaca acaccgccaa caggccgcgc acaatgcgcc
     2641 cttcgctgtt gccaaagaag tgcattttgc cgttttcagc cacggtatat cccagccaga
     2701 cgcggttttc gcatccggca atctctttag cctgcgcttt taactcgtct ggcaaagccg
     2761 gaagctgttt ccccagcatg atcaactggc gatatttatc ttcccattgc gacaacggtg
     2821 cgaaggtatt gcgtaacgtt tctgcggtta cggttgtgcc gaacggatgt ccggcgaatt
     2881 gcgggtttgt cattaatcca ccaataattc cagcgcgcgg tcaacggcat tcaccagcgc
     2941 atccacatca ctctttgtat tatatggcgc aaatgaggcg cgcagtgtgc cggttacgcc
     3001 taattctgcc agtagcggct gagcgcaatg ttgcccggcc cgcaaggcaa taccgtactc
     3061 cgccagtagc gtcaccatat cgctgtgatg aacgccagca aaatcaaagg ccagcaggct
     3121 ggaatcctgg cagcggaatg aacgaaagcc tggacgtttc gccagcgctt cttctgcaag
     3181 cgttgctaag ctacggctcc agctttcggc ctggttgata tcgtaatctg ccagccattc
     3241 cagcgccgcg cttaatccta tgacgccagc gacatttggc gttcctgctt ccagtttcca
     3301 cggcgcagat tgagtggtga aaccgtcaaa actcacttcg tgaatcattt tgccgccacc
     3361 cagccagggc gacatcgctt ccagcagttc tgatttgcca tacagcacgc cgatacccgt
     3421 cgggccatac agtttgtgac cagagaaagc atagaaatcg atatccagtt gctgaacatc
     3481 cgcggggaaa tgcaccgtcc cctgagcacc atcaaccatc accaccatcc cggctgaatg
     3541 agcaaaggta atcgctcgcg ccagatccgg gcaaccgcca gtcacgttcg acatctgacc
     3601 caacgccaga atccgactac ggggagtaat cagctctggc aacaaatcga catccggcag
     3661 tcgctgcgca ttaagcggca atttcaccac tttggctcta gtttgttggg cgaccatcag
     3721 ccaggggacg aggttagcgt ggtgttctgc cacgctgaca ataatctcat cgcccggttg
     3781 cagacgcgga cgcgcatagc attgtgccac catgttgatg gattcagtgg tgccgcgcgt
     3841 ccagacgata gttttatcat ccggtgcatt cagtaattgc gccactttct ctcgtgcagc
     3901 ttcataacgc gcggtcaggc gttgggcttc ggcaaactgg ctgcgatgga cgtttccggc
     3961 gctcaggctg taaaactgtc gagtggcttc aaccacggct tcaggtttaa gcgcggtcgc
     4021 ggcgctgtcg agatagacgc ccgcatcctg tagtgcggga aactgggcgc gaaactgcgc
     4081 gggattaaaa acgttcatgg tactcctcgg cttgatgcac cgatcgtgac gcaaaattca
     4141 ctttgataca aggagagtgc taaccatcag aattatctgg atatcctggc gaagccgcgt
     4201 aagtaacata tgctgaatag tgttaggcgt taattttagc ctgttaatca atactattaa
     4261 gatttattaa tcgttttaag gagaagatga tgaaaaagac tgccgcaatt atttctgcct
     4321 gtatgctgac ttttgccctg agcgcctgtt ccggttcgaa ctatgtgatg cacaccaatg
     4381 acggacgtac catcgtctct gacggcaaac cacagactga taacgatatc ggtatgattt
     4441 cgtataaaga cgctaatggc aacaaacagc agatcaaccg tactgacgtg aaagagatgg
     4501 tcgaactgga acagtaattc gcgatcgcaa ggtaaaaaaa agcaccgcaa ttaggcggtg
     4561 ctacattaat cactatggac agacagggta aatgtacagg aagtgaaaaa aggtagcttt
     4621 gctaccatgg tctgaatcgc agaccaattg caaacacaac aacacaacat cacaaccgta
     4681 agccaaaagt tcaccagaat acgcattccg ataaaacttt tcgttccggc tcaggaagtg
     4741 ccgccactat aggtatttgc tggtagaagc tcaacggaca atttataatg gctcagatta
     4801 aaaaaactaa taggttacac agtgtgatct aattgttaaa ttcatttaac atcaaagttt
     4861 aaaagccatg tctaaacgat taccaccgct aaatgcctta cgagtttttg atgccgcagc
     4921 acgccattta agttttactc gcgcagcaga agagcttttt gtgacccagg ccgcagtaag
     4981 tcatcaaatc aagtctcttg aggatttttt ggggctaaaa ctgttccgcc gccgtaatcg
     5041 ttcactcctg ctgaccgagg aagggcaaag ctatttcctc gatatcaaag agatattttc
     5101 gcaattaacc gaagcgacgc gtaaactcca ggcccgtagc gccaaggggg cgttgacggt
     5161 cagtttactc cccagtttcg ccattcattg gttggttccg cgactttcca gctttaattc
     5221 agcttatccg ggaattgacg ttcgaatcca ggcggttgat cgtcaggaag ataagctggc
     5281 ggatgatgtt gatgtggcga tattttatgg tcggggcaac tggccggggc tacgggtgga
     5341 aaaactgtac gccgaatatt tattgccggt gtgttcgccg ctactgctga caggcgaaaa
     5401 acccttgaag accccggaag atctggctaa acatacgtta ttacatgatg cgtcacgccg
     5461 tgactggcag acatataccc gacagttggg gtttaatcat atcaacgttc agcaagggcc
     5521 aatttttagt catagcgcca tggtgctgca agcggctatt cacgggcagg gagtggcgct
     5581 ggcaaataac gtgatggcgc aatctgaaat cgaggccgga cgtcttgttt gcccgtttaa
     5641 tgatgttctg gtcagtaaaa atgcttttta tctggtttgt catgacagcc aggcagaact
     5701 gggtaaaata gccgcctttc gccaatggat cctggcgaaa gccgctgctg aacaagaaaa
     5761 attccgcttt cgttatgaac aataatttac gtagggtacg accatgacca gccgttttat
     5821 gctgattttc gccgccatta gcggcttcat ttttgtggca ctgggcgctt ttggtgcgca
     5881 tgtgttaagt aaaaccatgg gggccgttga gatgggctgg atccaaaccg gtctcgaata
     5941 ccaggcgttt catacgctgg cgatcttagg tctggcggtg gcaatgcagc gtcgcatcag
     6001 tatctggttt tactggagta gcgttttcct cgcgttaggc acggtgctgt tcagcggcag
     6061 cctttattgc ctggcgctgt cccatctgcg tttgtgggcg tttgtcacac cggttggcgg
     6121 cgtgagcttc cttgcgggct gggcgttaat gttagttggt gctatccgtt taaagcgcaa
     6181 gggcgtaagt catgaataag gttgtattgc tgtgccgtcc gggctttgaa aaagagtgcg
     6241 ccgcagaaat taccgataaa gccggccagc gggaaatttt cggttttgcc cgcgtgaaag
     6301 agaatgcggg ttatgtcatt tatgaatgtt atcaacctga tgatggcgat aagttaatcc
     6361 gtgagctgcc gttcagttca ttaatttttg cccgccagtg gtttgtggtg ggggaactcc
     6421 tgcagcattt gccgccagaa gatcgtatta cccccattgt cggcatgtta cagggtgtag
     6481 tagagaaggg cggtgaactg cgtgttgaag ttgccgatac caacgaaagc aaagagttgc
     6541 tgaaattctg ccgtaagttc actgttccgc tacgcgctgc cttgcgcgat gctggggtgc
     6601 tggcgaacta tgaaacgccg aagcgtccgg ttgtgcatgt attctttatt gcaccaggat
     6661 gctgctatac cggttactca tacagcaaca ataattcgcc gttctatatg ggcattccgc
     6721 gcctgaaatt tccggcagag gcgccgagtc gttccacgct caaactggaa gaggcatttc
     6781 atgtgtttat tcctgcggat gagtgggatg aacgcctggc gaacgggatg tgggcggtgg
     6841 atttaggcgc ttgccctggc ggctggacct accaactggt gaagcgcaac atgtgggttt
     6901 attccgtcga caacggcccg atggcgcaaa gtctgatgga taccgggcag gtgacgtggc
     6961 tgcgggaaga cggtttcaaa ttccgtccga cacgcagcaa tatctcctgg atggtatgcg
     7021 atatggttga aaaaccggct aaagttgcgg cattgatggc gcagtggctg gttaatggct
     7081 ggtgccgtga aaccattttc aatctcaaac tgccgatgaa aaaacgctac gaagaagtat
     7141 cacacaatct ggcgtatatt caggcacagc ttgatgaaca tggcataaat gctcagattc
     7201 aggcgcggca gttgtatcac gatcgcgaag aagtgacggt gcacgtccgc cgcatctggg
     7261 ctgcggtggg tggtcgtcgc gacgagcgat aacaggcaag gccggggcgt cgtctccggc
     7321 ctgctaccct ttccgtcagg ctgttaccaa agaagttgca acctggcgct catcagaaat
     7381 aatgtgcgtt acctcatcca gatgcccgat gcgggcttcc cctgaacgat taaatttact
     7441 tttatcaatc aataacaacg attgcgcggc acgttttaat agcatcgatt tgtaatcagc
     7501 gttgatcgca ttggagtccc acagtgcgcc gctgctatcg atcccttcac aagaaaaaat
     7561 aaacagatcg atttccagcg atttaagttg ggaaatcagc gagggattga cgtaacagcc
     7621 atatttgcgc tcaagtgtgc cgccggaact gatcagttga atgcgttcgc gtttaccgag
     7681 ttcatggcaa atcggatggc tattggtgaa gacctgaatg ttgatgtcag gcaactggcg
     7741 tgccagatac cagcaagttg aactggcgtc taaggctatc accatccctt cttcaatcca
     7801 cgcgagcgcc tcgcgcgcga tatctgcttt atgcgcataa tggcttttca gcctgatgtg
     7861 aaagggatcg ccactgtctt gattttgacg gtggatatat ttagcgcgtc catgattgcg
     7921 cagaatttta ccctgcgtct gtaattcatt gagatcgcga cgaatggttt ctttactgac
     7981 ctttagctgt tcagagagag cttccgtggt caggctggta tggttcagca gcaggtcgac
     8041 tatcgcttgc tggcgtgccg ctttcatatt tttcacccca tcattcgata accgcccgcg
     8101 tcatgcgggc gtaccggtat gagattacgg tgttacccct ttttttaaaa gaatattccc
     8161 gtacttcgct cgttcgcctg tgataacgat cgcaaaggct ttttgcgccc gttcataaaa
     8221 cgcaaaacga ttgatgcgga tgatgtcagg acacggggct tgtagtgaaa gcgcattacg
     8281 gtaacgtcgt tctacttcag gatcgagagt gtcaccctct accgccgcca tcatcaccag
     8341 cggcggggca taactgtcca gctcaaataa cgggataatc gcctggagca agtcgctcac
     8401 caacaggcca tcagcgcgga tcacctgcgg ttccatcgaa tgggcgggaa agtgagcatc
     8461 ggaaaaaata atttcatctc catgtcccat ctctgccagc actttcaata gttcgggaga
     8521 aattaacggc gaaattgttt tcagcatttc acacttcctc tataaattca ggttcagttt
     8581 gcgggtagaa ataacggtac tgataatgaa tctgtgcacg ggcttcttcc gggctgttaa
     8641 attcccctac gccataccaa ccgaacagcg cagctcctgc gacggtcgtt tcggcatcgt
     8701 cgagaacttt taccggaata tcaagcatat tggctttaat ctgattccac aatgtgttgc
     8761 gacttcctcc accgactaac aataattcat aggctttaaa gtgcccgatt ttttccagca
     8821 tctgtagatt gcgctgtaat tgcgcagtta acccttccag cgccgcgcga tagaaatgcc
     8881 cccgcgtggt attaagcgtc actccttgcc agccagcgtt ctgacacgac aataaatcac
     8941 actgcatttt cacaccatcc gcgccaggtg cgatcagacg agcttcttca atcaacattt
     9001 gccagggtgt ttcagccgtc cagaacagtt ttctcaccca ttccagcacg ccggatgcca
     9061 gccattgcat acctgggtta tacaaccatg cctggctatc tagttcgcag gtggaaccgg
     9121 cgtactgact taacagcgaa gtatcaacct aggcgctgcg aaccattaaa atttcccatg
     9181 taccggagga gagcacgggt tcattttgtt cggcaccagc gccaaaaagg gcgaactggg
     9241 tatcgtgacc tgcggaaatc accggtatgc caacgggtaa gccgagcatt gctgcggcgt
     9301 tgttctgtag cgtaccaatc tgttcacccg cttccaccag acgagggaag agtcggcgtg
     9361 gaataccggt ggcttgtaaa atttgcggac tgaaatcgcg ttgctggata tccagcatct
     9421 ggctggttcc ggccatcgtg atatcagtag tgaattcgct ggttaaacgg tggttaatca
     9481 gcgacgaaat gaagagccag gcgtgcgcgc gttccagcag ttgtggatga ttttctttca
     9541 accacaccaa cttatataac gtattgaaac taaaggctcc gacgccagaa atagcctgca
     9601 accgctgtgc ggagattaac cgttcaatat tgtccataac cgctgctgtt cgcggacatt
     9661 tccagctaat gatcggatag agcagattgc cttgcttatc taccagagcg ccatccacac
     9721 taaaggtggt gacggcgata ccgcggatgt ggcattcagt cagttcacta ttgatttgcc
     9781 gacagcaatc agcaaagcgt tgcaaaatgg cagtctaaag accactggtg ccaggtgttg
     9841 ttttccatcg cgatatcgct ggcattaggc gttgaggcgc gggcaacaat ttttccctgc
     9901 cgattaaccg cgatggccct gacattggtc gcgccacagt cgagtaccag gataacttct
     9961 tgtttcataa tcgctcctgc aatatagccg gataacattg tttatccggc taaccactct
    10021 tgcttgttca ggcccggtag ccggagcgac cgggcatcac atcagggtgt aatgtattaa
    10081 cgcttgtaca acggaccgta gttctggcaa gcgcggtaat cctggccttc aatatccatg
    10141 ccgtgcgcag cccaggcaga aggacgatac actttggtct cttcaacgtt gtgcatacat
    10201 accgggatac gcagcatgga ggcaagggtg ataaagtctg cgccaacgtg gccgatggtc
    10261 agaactccgt ggttggcacc ccagttcgcc attaccgaat acacatccgt aaacggccct
    10321 ttaccagtga ggcgcggtgc aaaccaggtg gttggccagg ttgagttggt gcgtttgttg
    10381 aggatgtcat gcacatcctt cggcaattcc acgctccagc cttccgcgat ttgcagtacc
    10441 ggtcccaggc ctttgatgat gttgacacga gtcatggtga acgggacgcc gccttcggta
    10501 aggaagcggg aagagtaacc gccgccacgg aagtattcgt ggatcgccgg gcaccattcg
    10561 gtagcggcga ggcaagcgtc agcctcttgc tgagagattt cccagtgcgg cttcatcgtc
    10621 gggttacctt cgctgtcgcg ttgtttacag gaaccgtcca gcgcagcaga acctgagttg
    10681 atcaaatgga tgatgccgtg ttctgccagt ccgtccagtt tatgccccgt tacacgctca
    10741 attgcttctg gtgaccagta ggtacgcaca tcggcaaata cctgagcggt gccggtgagc
    10801 tggtgaccca ttagcattgc cacgccgtta agactgtcgt tttcggtcgc cacgacaaag
    10861 ggttcgcgca cgccattcca gtcaaatgaa ctgttgagga tcgcttcggc ggtgtcacca
    10921 ttgggatatt gatcggtcca gtgacgttgc ccctggaagc ccgcagcgat ggcgttgtag
    10981 ccaagtgatt cttccacgcg accaatatcg gccagtttgc tgttgccttg catcatgtcg
    11041 cggatacaca tcgccatcag taaactttcg cgcagaactg cgcggctttg ctcggcattg
    11101 cgttgatact gtttgttatt ttcatcttcg ccatagcgga agtttttatc agcccaggcc
    11161 agtgccattt ccagttcggc ttcgtcgtaa atcttctgat cgatacggcg acgcagttcg
    11221 gtcatatcca ccgcctggac tttcattccc agccaggatt caaagaagtt gtgatcaaca
    11281 atagaaccgg cgatacccat cgaaacgccg cccagcgaca gatagctttt acctttcatg
    11341 ctggcgacgg ccaaaccggc gcgggcaaag cgcagcagtt tttcttcaac atcggcagga
    11401 atcgatgtgt catcggcatc ctgaacgtca tgaccgtaaa tggagaatgc cgggatgcct
    11461 ttctggctgt gagctgccag agccgcagcc aggtaaacag cgccggggcg ttcagtgccg
    11521 ttaaagcccc aaatggcttt cgggcgggtt ggatccatgt cgatggtttc actgccatag
    11581 caccagcaag gcgttaccgt aatggtgagg cctacattct ggctgctgaa tttttcttcg
    11641 caagcagcgg cttcagccat acccgcgata caggtatcgg aaatgacaca ctcgacggca
    11701 gctccgcagg catggcgcag tttctcggtc agcagtgcgg ccgtagcttt cgccatattc
    11761 attgtttgtt cttcaagcga ctcacgaaca cccatgcgac gaccgtcaat aaccgggcgg
    11821 ataccaattt tcggtaagct gatttttttc attacagatt cctcacttta ttcggaaaat
    11881 atgttcagtt agttgccgtt tgagaacgga aacgggcaaa gataaagatg accgcgaagc
    11941 agagtgcggg gatcagttca gcagtgggga tgttgcccgc cgcgtcactg acaaaaccca
    12001 tgaccggagt gacaataccg ccgccaataa tggtcataac gatgaaggac gaaccgtatt
    12061 tggtgtcctg gccgagattc ttaatgccca gcgagaagat tgttgggtac tgaatcgaca
    12121 taaaggcgct gcataaagtc agggctatta agcccacatg accgccagcg aaggctgaga
    12181 tcaggcacag tgccatagcg attaatgcgt aggcggccag gactttgtgt ggtgcgaagc
    12241 gactgatgag ccaggtaccg gtgaaacgac caataaagaa gcacaccatg gttccggtta
    12301 aatagttagc ggcaaagcct gcagtcatac ctagaatttc ttctacagcg tagcgaatca
    12361 aatagctcca gcaggccgtt tgtgcgccga catagcagaa ttgcgctaat accgcccagc
    12421 gccagtgacg aatacgcgcc agacgagaaa gcgatgcgga gaacgatcct tgtttggcgt
    12481 cactgtgatt atcactctgc aatgccggga atttcgtcag catgatcagc agggcgacca
    12541 gtaacacgat agccacgatg atcatataag gtgtctgtac cgataatacc aggctgtgtt
    12601 tatacgcact caattgctct ggagacattt tatcgagaac gtcttgcgat tgatgtggca
    12661 cgttagacaa aataagactt tgcccaaaga caaccgcgat aattgcgcca aacgagttaa
    12721 atgtttgcgc aagatttaag cggaagtgac tactgctttc cggccctaat accgtaacaa
    12781 aaggggttgc ggcagtttcc agacaaccta atccggctgc aataataaat aggccaacta
    12841 aaaataaggt gtagttcatt atttctgcag cgggccagaa taatgcagcg cccaaggcat
    12901 ataaaaataa tccggtaata atccctgctt tataactgag ttttttcatc aatatcccag
    12961 cagggattgg gataatgaaa taaccaaagt aaaaggccga ttggatcagg ccagcctgga
    13021 aatttgtcag cgtaaaagcc tgctggaatt gaggtaataa aatgtcgtta aggttattgg
    13081 ctaccgccca aagaaaaaac agtgagcaca gcagcgcgaa tggaataatg taacttctgc
    13141 tttgccctgc atctttatct accgcacggt aactctgcgt ttgtattgat gtgtttccca
    13201 tagcatcctc ttaggttcag aagcttaagc acgtaatcgg ttatgttttc aaaagtgcaa
    13261 aaccagcgag ttcgggcttc atatcggtga atatttgcct gtctgaaatg aactcatgga
    13321 ttcattttca aataaaaaac gctctgtaat atgacggcgg tcacacttat tagctggaag
    13381 gtgactttat gtgactacca tcactttaat aatcgaaatt tcatgcccgt ttcagaaaat
    13441 aaatcataaa aaacacaaac cagatgaccg tattaattgt ttgtgccgaa aacgggcatt
    13501 tctggcgagg ggaaatggaa aaatgaccgt tttgataaat atttttaaga gcggtcattt
    13561 ttaacggggt tataaaaaga gaacttgcag ttatttactg tgattagttg agctaggtca
    13621 caaaatataa gcttgtgaat ttctttcttg ttaatcagga agaagatggt gggtaattaa
    13681 acggctaatt caatagtgtg aaaggaacaa cattattgcc ctgttttgaa tcagagagag
    13741 gtagctaatg gaacgaaata aacttgctcg tcagattatt gacacttgcc tggaaatgac
    13801 ccgcctggga ctgaaccagg ggacagcggg gaacgtcagt gtacgttatc aggatgggat
    13861 gctgattacg cctacaggca ttccatatga aaaactgacg gagtcgcata ttgtctttat
    13921 tgatggcaac ggtaaacatg aggaaggaaa gctcccctca agcgaatggc gtttccatat
    13981 ggcagcctat caaagcagac cggatgccaa cgcggttgtt cacaatcatg ccgttcattg
    14041 cacggcagtt tccattctta accgatcgat ccccgctatt cactacatga ttgcggcggc
    14101 tggcggtaat tctattcctt gcgcgcctta tgcgaccttt ggaacacgcg aactttctga
    14161 acatgttgcg ctggctctca aaaatcgtaa ggcaactttg ttacaacatc atgggcttat
    14221 cgcttgtgag gtgaatctgg aaaaagcgtt atggctggcg catgaagttg aagtgctggc
    14281 gcaactttac ctgacgaccc tggcgattac ggacccggtg ccagtgctga gcgatgaaga
    14341 gattgccgta gtgctggaga aattcaaaac ctatgggtta cggattgaag agtaatttcg
    14401 taaagcaaca aggagaagga tgatggctaa cagaatgatt ctgaacgaaa cggcatggtt
    14461 tggtcggggt gctgttgggg ctttaaccga tgaggtgaaa cgccgtggtt atcagaaggc
    14521 gctgatcgtc accgataaaa cgctggtgca atgcggcgtg gtggcgaaag tgaccgataa
    14581 gatggatgct gcagggctgg catgggcgat ttacgacggc gtagtgccca acccaacaat
    14641 tactgtcgtc aaagaagggc tcggtgtatt ccagaatagc ggcgcggatt acctgatcgc
    14701 tattggtggt ggttctccac aggatacttg taaagcaatt ggcattatca gcaacaaccc
    14761 ggagtttgcc gatgtgcgta gcctggaagg gctttccccg accaataaac ccagtgtacc
    14821 gattctggca atccccacca cagcaggtac tgcggcagaa gtgaccatta actacgtgat
    14881 cactgacgaa gaaaaacggc gcaagtttgt ttgcgttgat ccgcatgata tcccgcaggt
    14941 ggcgtttatt gatgctgaca tgatggatgg tatgcctccg gcgctgaaag ccgcgacggg
    15001 tgttgatgcg ctcactcatg ctattgaggg gtatattacc cgtggcgcgt gggcactaac
    15061 cgatgcactg cacattaaag cgattgaaat cattgctggg gcgctgcgag gatcggttgc
    15121 tggtgataag gatgccggag aagaaatggc gctcgggcag tatgttgcgg gtatgggctt
    15181 ctcgaatgtt gggttagggt tggtgcatgg tatggcgcat ccactgggcg cgttttataa
    15241 cactccacac ggtgttgcga acgccatcct gttaccgcat gtcatgcgct ataacgctga
    15301 ctttaccggt gagaagtacc gcgatatcgc gcgcgttatg ggcgtgaaag tggaaggtat
    15361 gagcctggaa ggggcgcgta atgccgctgt tgaagcggtg tttgccctca accgtgatgt
    15421 cggtattccg ccacatttgc gtgatgttgg tgtacgcaag gaagacattt cggcactggc
    15481 tcaggcggca ctggatgatg tttgtaccgg tggcaacccg cgtgaagcaa cgcttgagga
    15541 tattgtagag ctttaccata ccgcctggta aatgcgctga tgtgataatg ccggatacga
    15601 tttgcggcgt atccggctcg ccgttaccgt accaaccgca attgctgtaa attgccgtcg
    15661 atatgcaaat cggtttgtaa gcgggcaata tcgcggcaca gaaacgccat ctctttatgg
    15721 gtttctaatt ttttgcgcca cttttcggca accgcatcca gattctcata tatcccttcc
    15781 agactctgaa actcgaccag cagctgcgtg gcgctttttg gtccgattcc cgcaacaccc
    15841 ggtacctttg aactgctgat ccccgcaagt ccccagtaat cgggcaactg ctgcggttga
    15901 acgccaaatt ctttatcgat aaatggcgca tccagccaac gtttctggaa gtaatcacga
    15961 atacgtaatg tcggtgaaag taactgacag tagcctttat ctgtcgaaac aatcgttgcc
    16021 tgatgcccgg cctgtgtcac tttgaccgcc agtgtggcgg ctaagtcatc ggcttcgttg
    16081 ccgctggctg accagcacgg gacgccgcgt tgctcaaagg cggcgcgtaa tgcaggcatc
    16141 tcgtcgtgca actcttccgg cattggcggt cgatccgctt tgtaatctgg taaacgctga
    16201 tgacgccagc cgctactgcg gttttcatca tcaaaaacgg cgaccgcgtg ggttggctgg
    16261 ctgtgcataa tgagctgatc gagcgcatgc tggcaggttt cgacacaggg cgaaccctga
    16321 acggcatgaa tgcgacggat aagattcagt gcatcgacaa taagcaaatg aacagccacg
    16381 ataaccccct taaagatcag gcgataaggg taacattcga tgccggaaca ggctatggct
    16441 ggttgagaaa acaggaagag gcttcgcaaa acgaggcctc tgggagagat taatcgcagg
    16501 caacgatctt catcgccagg ccgccgcgag aggtttcgcg gtacttggcg ttcatgtctt
    16561 tacctgtttc gtacatggtt tcgataactt tatcgaggca gacgcgcggt tcgctggtac
    16621 ggcgcagtgc catacgcgcg gcgttcaccg ctttcactgc tgcaatggcg ttacgctcaa
    16681 tgcacggtac ctgaacctgc cccgcgaccg ggtcacaagt cagaccgagg ttgtgctcca
    16741 tcgcgatttc cgctgcgatg caaacctgcg ccgggcttgc gcctaacagt tctgccagac
    16801 cagctgccgc catagagcac gccacgccaa cttcaccctg gcaacccact tcggcaccag
    16861 aaatcgacgc gttcatctta taaagagaac caatggcgct ggctaccagc aggtaacgag
    16921 ccagtgagtt agcgttcact tcgcggataa acttatcgta gtacgccagc acagccggga
    16981 taatcccgca cgcaccgtta gtcggcgcag tcaccacgcg accgccagca gcgttctctt
    17041 cgttcactgc cagtgcaaac atgttgatcc agtcaacaac cgccatcggg tcagtggtgg
    17101 ttttatcctg gctgaccagc atccggcgta gtgccgcagc acggcgtgga acgcgcagtt
    17161 tgccaggcaa cacgccttcg gtggaaatac cgcgctcaat accgccacgc atgacttccc
    17221 agacgttcgc caggtgctgt tccagctctt ctttactgtg cagcgccagc tcgtttttca
    17281 tcatcaggcc agagagtgac agcccggttt cctgacaatg tttttgcaga tcggctgctg
    17341 aactgtacgg ataaggaact tcaaccggtg cgctatcctg ctggccaaaa tgctcttcat
    17401 caacgataaa gccaccgcca atagagtagt aagtctggct gtaaacgact ttatcgcccg
    17461 ccagcgcggt aatgcgcata ccgttttcat gcagagaaag gttgtctgca tggaagttca
    17521 tgcactgatc aaccgggaac tccacttcat gctgaccgtt tgccagcatc aggcgaccat
    17581 gagtattcac atcctgaata aaaccgggga tggaatcgat atccacggta tccggcaggt
    17641 tacccgccag gcccataata atggcgatat ctgtgtggtg gcctttacca gtcagagaga
    17701 gcgagccgta cacgtcaacc accacgcggg tcacgtcttt aagcaggtta cgggcaatca
    17761 gatcgtcggt aaattgttta cccgctttca ttggtccaac ggtatgagaa ctggaagggc
    17821 caatgccgat tttgaaaata tcgaatacgc taatcatagg aaatacatcg cgttaaaacg
    17881 gaggaagcgc cgcccgaaag cggcgcgaaa ggacttagct gaacagagag tagaagattg
    17941 cggagattgc aatcagaccc atcacgacaa cgaatacgtt gctgatgtga ccgctgtact
    18001 tacgcattgc cggtactttc tgaattgcgt acatcggcat caggaacagg atcatcgcga
    18061 tgattgggcc gcccagggtt tcaatcatac ccaggatgct cgggttcagg gtggcaacaa
    18121 tccaggtcgt taccagcatg aacagcgcag tgatacggtt cagcttgttg atttcgatag
    18181 acttaccttt accacgcaga gatttaatca ccataccgtt gaagccttca cgtgcgccca
    18241 ggtagtggcc gaggaaggat ttggtgatag cgataatcgc gataatcgga gccatccacg
    18301 cgataaccgg tgcgttaaag tggttagcca ggtaagacag aatcgagatg ttctgctctt
    18361 tagccgcagc caggtctgcc ggagtcaggc tcagtacgca gctgaagacg aagaacatta
    18421 cggtcagcac catcatgatg tgtgcgaatg ccaggatctt cgagcatttc tgttctgcca
    18481 tatcgccgta ctcttcacgc ttcgcaacgg cgaaagaaga gatgatcgga gagtggttga
    18541 acgagaacac cattaccgga attgccagcc acagggtcat ccacagaccg tttccggttg
    18601 cagatgcagt gtccagagac agcgtttcca gtgctgcgcc gttccactgc gggatcaggt
    18661 acagagccag cagcatcagt acgccaacaa acgggaatac cagaatactc atcgctttaa
    18721 cgatcatctg ctcaccaaag cgaacgatgg tcatcatacc cacgatcagg atcagcgaca
    18781 gaatcgcacg cggcggtggc gtcataccca gctggtgaga catgaagctt tcaacggtat
    18841 tggtgattgc cacgctataa accagcagga tcgggtagat agcgaagaag tagagcaggg
    18901 taatcagttt acctgcgcca ataccaaagt gttcttctac aacctcggtg atgtcttcgc
    18961 ccgggttttt accagacagt acgaagcgag tcaggccgcg gtgagcaaaa aacgtcatcg
    19021 ggaacgcaag gatagccatg atgatcagcg ggatcatacc gccaacaccg gcgttgattg
    19081 gcaggaacag cacgcccgcg ccgattgccg tgccgtaaag gcccagcatc cacatggtgt
    19141 ctgtcttgcg ccaggcacta cgagagtctt tcgacgcaat cgtgctggtt tgagtcgttt
    19201 ccatctattt ctcctggagg aatgcaaata attcagcttt tagccagttc agcccgggaa
    19261 ggtgcctgac cgtaaaaatg aaatctgtct atcggcgatg gcttaatcat ttaccgccgt
    19321 aataatttga gggcggaaag atacacacaa gttatgtatc tatcagtgat cttgatccca
    19381 aatacaataa tctgcttgga agatagatta tcccaacgct ttactctgta aatcaaaagt
    19441 aaaagagaat ttaagggcaa tatttgccca ttaaacgaca tctgacttct tccggggcgc
    19501 gcaatatatg taggttgaac tgaaaaaatc aatactttta gttaatcatg gcgataattt
    19561 ctttttcata gcgataaaat acggtttatg aaaataggga aacgtttgtg ttgcaacata
    19621 aattgtatga catattaact gtgatgatat ttgtcgcgtg agggtggtaa gaaacattca
    19681 tataacaaat gaaatgagtt gatgagtaag tgaccaaaaa cgagaaaacc cggcaacaaa
    19741 cgtcaccg
//
LOCUS       DAZPAB010000063        19737 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZPAB010000063 DAZPAB010000000
VERSION     DAZPAB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19737
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.063"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            301..722
                     /gene="atpI"
                     /locus_tag="ACHUPG_001975"
                     /pseudo
     CDS             301..722
                     /gene="atpI"
                     /locus_tag="ACHUPG_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
     gene            731..1546
                     /gene="atpB"
                     /locus_tag="ACHUPG_001976"
     CDS             731..1546
                     /gene="atpB"
                     /locus_tag="ACHUPG_001976"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR0861603.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            1593..1832
                     /gene="atpE"
                     /locus_tag="ACHUPG_001977"
     CDS             1593..1832
                     /gene="atpE"
                     /locus_tag="ACHUPG_001977"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR0861604.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            1894..2364
                     /gene="atpF"
                     /locus_tag="ACHUPG_001978"
     CDS             1894..2364
                     /gene="atpF"
                     /locus_tag="ACHUPG_001978"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR0861605.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            2379..2912
                     /gene="atpH"
                     /locus_tag="ACHUPG_001979"
     CDS             2379..2912
                     /gene="atpH"
                     /locus_tag="ACHUPG_001979"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR0861606.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            2925..4466
                     /gene="atpA"
                     /locus_tag="ACHUPG_001980"
     CDS             2925..4466
                     /gene="atpA"
                     /locus_tag="ACHUPG_001980"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR0861607.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            4517..5380
                     /gene="atpG"
                     /locus_tag="ACHUPG_001981"
     CDS             4517..5380
                     /gene="atpG"
                     /locus_tag="ACHUPG_001981"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR0861608.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            5407..6789
                     /gene="atpD"
                     /locus_tag="ACHUPG_001982"
     CDS             5407..6789
                     /gene="atpD"
                     /locus_tag="ACHUPG_001982"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR0861609.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            6810..7229
                     /locus_tag="ACHUPG_001983"
     CDS             6810..7229
                     /locus_tag="ACHUPG_001983"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR0861610.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            7580..8950
                     /gene="glmU"
                     /locus_tag="ACHUPG_001984"
     CDS             7580..8950
                     /gene="glmU"
                     /locus_tag="ACHUPG_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR0861611.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            9112..10941
                     /gene="glmS"
                     /locus_tag="ACHUPG_001985"
     CDS             9112..10941
                     /gene="glmS"
                     /locus_tag="ACHUPG_001985"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR0861612.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            11103..11924
                     /locus_tag="ACHUPG_001986"
     CDS             11103..11924
                     /locus_tag="ACHUPG_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFR0861613.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            11911..14019
                     /locus_tag="ACHUPG_001987"
     CDS             11911..14019
                     /locus_tag="ACHUPG_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR0861614.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            14016..15683
                     /locus_tag="ACHUPG_001988"
     CDS             14016..15683
                     /locus_tag="ACHUPG_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0861615.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            15686..17212
                     /locus_tag="ACHUPG_001989"
     CDS             15686..17212
                     /locus_tag="ACHUPG_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFR0861616.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            17213..18829
                     /locus_tag="ACHUPG_001990"
     CDS             17213..18829
                     /locus_tag="ACHUPG_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFR0861617.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(18885..19037)
                     /locus_tag="ACHUPG_001991"
                     /pseudo
     CDS             complement(18885..19037)
                     /locus_tag="ACHUPG_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(19060..19437)
                     /locus_tag="ACHUPG_001992"
     CDS             complement(19060..19437)
                     /locus_tag="ACHUPG_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861618.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
BASE COUNT         5149 a         4416 c         5149 g         5023 t
ORIGIN      
        1 gccaagtaaa taaatatgct gtgcgcgaac atgcgcaata tgtgatctga agcacgcttt
       61 atcaccagtg tttacgcgtt atttacagtt tttcatgatc gaacaggggt agcggaaaag
      121 tcgcaattgt atgcactgga aaaatattta aacatttatt caccttttgg ctacttattg
      181 tttgaaatca cgggggcgca ccgtataatt tgaccgcttt ttgatgcttg actctaagcc
      241 ttaaagaaag ttttatacga cacgcggcat acctcgaagg gagcaggagt gaaaaacgtg
      301 atgtctgtgt cgctcgtgag tcgaaacgtt gctcggaagc ttctgctcgt tcagttactg
      361 gtggtgatag caagtggatt gctgttcagc ctcaaagacc ccttctgggg cgtctctgca
      421 ataagcgggg gcctggcagt ctttctgcct aacgttttgt ttatgatatt tgcctggcag
      481 tctttctgcc taacgttttg tttatgatat ttgcctggcg tcaccaggcg catacaccag
      541 cgaaaggccg ggtggcttgg acattcgcat ttggcgaagc tttcaaagtt ctggcgatgt
      601 tggtgttact ggtggtggcg ttggcggttt taaaggcggt attcttgccg ctgatcgtta
      661 cgtgggtttt ggtgctggtg gttcagatac tggcaccggc tgtaattaac aacaaagggt
      721 aaaaggcatc atggcttcag aaaatatgac gccgcaggat tacataggac accacctgaa
      781 taaccttcag ctggacctgc gtacattctc gctggtggat ccacaaaacc ccccagccac
      841 cttctggaca atcaatattg actccatgtt cttctcggtg gtgctgggtc tgttgttcct
      901 ggttttattc cgtagcgtag ccaaaaaggc gaccagcggt gtgccaggta agtttcagac
      961 cgcgattgag ctggtgatcg gctttgttaa tggtagcgtg aaagacatgt accatggcaa
     1021 aagcaagctg attgctccgc tggccctgac gatcttcgtc tgggtattcc tgatgaatct
     1081 gatggattta ctgcctatcg acttgctgcc gtacattgct gaacatgtac tgggtctgcc
     1141 tgcactgcgt gtggttccgt ctgcggacgt gaacgtaacg ctgtctatgg cactgggcgt
     1201 atttatcctg attctgttct acaacatcaa aatgaaaggc atcggcggct tcacgaaaga
     1261 gttgacgctg cagccgttca atcactgggc gttcattcct gtcaacttaa tccttgaagg
     1321 ggtaagcctg ctgtccaaac cagtttcact cggtttgcga ctgttcggta acatgtatgc
     1381 cggtgagctg attttcattc tgattgctgg tctgttgccg tggtggtcac agtggatcct
     1441 gaatgtgccg tgggccattt tccacatcct gatcattacg ctgcaagcct tcatcttcat
     1501 ggttctgacg atcgtctatc tgtcgatggc gtctgaagaa cattaattta ccaacactac
     1561 tacgttttaa ctgaaacaaa ctggagactg tcatggaaaa cctgaatatg gatctgctgt
     1621 acatggctgc cgctgtgatg atgggtctgg cggcaatcgg tgctgcgatc ggtatcggca
     1681 tcctcggggg taaattcctg gaaggcgcag cgcgtcaacc tgatctgatt cctctgctgc
     1741 gtactcagtt ctttatcgtt atgggtctgg tggatgctat cccgatgatc gctgtaggtc
     1801 tgggtctgta cgtgatgttc gctgtcgcgt agtaagcgtt gcttttattt aaagagcaat
     1861 atcagaacgt taactaaata gaggcattgt gctgtgaatc ttaacgcaac aatcctcggc
     1921 caggccatcg cgtttgtcct gttcgttctg ttctgcatga agtacgtatg gccgccatta
     1981 atggcagcca tcgaaaaacg tcaaaaagaa attgctgacg gccttgcttc cgcagaacga
     2041 gcacataagg accttgacct tgcaaaggcc agcgcgaccg accagctgaa aaaagcgaaa
     2101 gcggaagccc aggtaatcat cgagcaggcg aacaaacgcc gctcgcagat tctggacgaa
     2161 gcgaaagctg aggcagaaca ggaacgtact aaaatcgtgg cccaggcgca ggcagaaatt
     2221 gaagccgagc gtaaacgtgc ccgtgaagag ctgcgtaagc aagttgctat cctggctgtt
     2281 gctggcgccg agaagatcat cgaacgttcc gtggatgaag ctgctaacag cgacatcgtg
     2341 gataaacttg tcgctgaact gtaaggaggg aggggctgat gtctgaattt attacggtag
     2401 ctcgccccta cgccaaagca gcttttgact ttgccgtcga acaccaaagt gtagaacgct
     2461 ggcaggacat gctgacgttt gccgccgagg taaccaaaaa cgaacaaatg gcagagcttc
     2521 tctctggcgc gcttgcgcca gaaacgctcg ccgagtcgtt tatcgcagtt tgtggtgagc
     2581 aactggacga aaacggtcag aacctgattc gggtaatggc tgaaaatggt cgtcttaacg
     2641 cgctcccgga tgttctggag cagtttattc accttcgtgc cgtgagtgag gctaccgctg
     2701 aggtagacgt catttccgct gccgcactga gtgaacaaca gctcgcgaaa atttctgctg
     2761 cgatggaaaa acgtctgtca cgcaaagtta agctgaattg caaaatcgat aagtctgtaa
     2821 tggctggcgt tatcatccga gcgggtgata tggtcattga tggcagcgta cgcggtcgtc
     2881 ttgagcgcct tgcagacgtc ttgcagtctt aaggggactg gagcatgcaa ctgaattcca
     2941 ccgaaatcag cgaactgatc aagcagcgca ttgctcagtt caatgttgtg agtgaagctc
     3001 acaacgaagg tactattgtt tctgtaagtg acggtgttat ccgcattcac ggcctggccg
     3061 attgtatgca gggtgaaatg atctccctgc cgggtaaccg ttacgctatc gcactgaacc
     3121 tcgagcgcga ctctgtaggt gcggttgtta tgggtccgta cgctgacctt gccgaaggca
     3181 tgaaagttaa gtgtactggc cgtatcctgg aagttccggt tggccgtggc ctgctgggcc
     3241 gtgtggttaa cactctgggt gcaccaatcg acggtaaagg tccgctggat cacgacggct
     3301 tctctgctgt agaagcaatc gctccgggcg ttatcgaacg tcagtccgta gatcagccgg
     3361 tacagaccgg ttataaagcc gttgactcca tgatcccaat cggtcgtggt cagcgtgaat
     3421 tgatcatcgg tgaccgtcag acaggtaaaa ccgcactggc tatcgatgcc atcatcaacc
     3481 agcgcgattc cggtatcaaa tgtatctatg tcgctatcgg ccagaaagcg tccaccattt
     3541 ctaacgtggt acgtaaactg gaagagcacg gcgcactggc taacaccatc gttgtggtag
     3601 caaccgcgtc tgaatccgct gcactgcaat acctggcacc gtatgccggt tgcgcaatgg
     3661 gcgaatactt ccgtgaccgc ggtgaagatg cgctgatcat ttacgatgac ctgtctaaac
     3721 aggctgttgc ttaccgtcag atctccctgc tgctccgtcg tccgccagga cgtgaagcat
     3781 tcccgggcga cgttttctac ctccactctc gtctgctgga gcgtgctgca cgtgttaacg
     3841 ccgaatacgt tgaagccttc accaaaggtg aagtgaaagg gaaaaccggt tctctgaccg
     3901 cactgccgat tatcgaaact caggcgggtg acgtttctgc gttcgttccg accaacgtaa
     3961 tctccattac cgatggtcag atcttcctgg aaaccaacct gttcaacgcc ggtattcgtc
     4021 ctgcggttaa cccgggtatt tccgtatccc gtgttggtgg tgcagcacag accaagatca
     4081 tgaaaaaact gtccggtggt atccgtaccg ctctggcaca gtatcgtgaa ctggcagcgt
     4141 tctctcagtt tgcatccgac cttgacgatg caacacgtaa gcagcttgac cacggtcaga
     4201 aagtgaccga actgctgaaa cagaaacagt atgcgccgat gtccgttgcg cagcagtctc
     4261 tggttctgtt cgcagcagaa cgtggttacc tggcggatgt tgaactgtcg aaaattggca
     4321 gcttcgaagc cgctctgctg gcttacgtcg accgtgatca cgctccgttg atgcaagaga
     4381 tcaaccagac cggtggctac aacgacgaaa tcgaaggcaa gctgaaaggc atcctcgatt
     4441 ccttcaaagc aacccaatcc tggtaacgtc tggaggcttg ccttagggca ggccgcaagg
     4501 cattgaggag aagctcatgg ccggcgcaaa agagatacgt agtaagatcg caagcgtcca
     4561 gaacacgcaa aagatcacta aagcgatgga gatggtcgcc gcttccaaaa tgcgtaaatc
     4621 gcaggatcgc atggcggcca gccgtcctta tgcagaaacc atgcgcaaag tgattggtca
     4681 ccttgcacac ggtaatctgg aatataagca cccttacctg gaagaccgcg acgttaaacg
     4741 cgtgggctac ctggtggtgt cgaccgaccg tggtttgtgc ggtggtttga acattaacct
     4801 gttcaaaaaa ctgctggcgg aaatgaagac ctggaccgac aaaggcgttc aatgcgacct
     4861 cgcaatgatc ggctcgaaag gcgtgtcgtt cttcaactcc gtgggcggca atgttgttgc
     4921 ccaggtcacc ggcatggggg ataacccttc cctgtccgaa ctgatcggtc cggtaaaagt
     4981 gatgttgcag gcctacgacg aaggccgtct ggacaagctt tacattgtca gcaacaaatt
     5041 tattaacacc atgtctcagg ttccgaccat cagccagctg ctgccgttac cggcatcaga
     5101 tgatgatgat ctgaaacata aatcctggga ttacctgtac gaacccgatc cgaaggcgtt
     5161 gctggatacc ctgctgcgtc gttatgtcga atctcaggtt tatcagggcg tggttgaaaa
     5221 cctggccagc gagcaggccg cccgtatggt ggcgatgaaa gccgcgaccg acaatggcgg
     5281 cagcctgatt aaagagctgc agttggtata caacaaagct cgtcaggcca gcattactca
     5341 ggaactcacc gagatcgtct cgggggccgc cgcggtttaa acaggttatt tcgtagagga
     5401 tttaagatgg ctactggaaa gattgtccag gtaatcggcg ccgtagttga cgtcgaattc
     5461 cctcaggatg ccgtaccgcg cgtgtacgat gctcttgagg tgcaaaatgg taatgagcgt
     5521 ctggtgctgg aagttcagca gcagctcggc ggcggtatcg tgcgtaccat cgcaatgggt
     5581 tcctccgacg gtctgcgtcg cggtctggat gtaaaagacc tcgaacaccc gatcgaagtc
     5641 ccggtaggta aagcgactct gggccgtatc atgaacgtac tgggtgaacc ggtcgacatg
     5701 aaaggcgaga tcggtgaaga agagcgttgg gcgattcacc gcgcagcacc ttcctacgaa
     5761 gagctgtcaa actctcagga actgctggaa accggtatca aagttatcga cctgatgtgt
     5821 ccgttcgcta agggcggtaa agttggtctg ttcggtggtg cgggtgtagg taaaaccgta
     5881 aacatgatgg agctcattcg taacatcgcg atcgagcact ccggttactc tgtgtttgcg
     5941 ggcgtaggtg aacgtactcg tgagggtaac gacttctacc acgaaatgac cgactccaac
     6001 gttatcgaca aagtatccct ggtgtatggc cagatgaacg agccgccggg aaaccgtctg
     6061 cgcgttgctc tgaccggtct gaccatggct gagaaattcc gtgacgaagg tcgtgacgtt
     6121 ctgctgttcg ttgacaacat ctatcgttac accctggccg gtacggaagt atccgcactg
     6181 ctgggccgta tgccttcagc ggtaggttat cagccgaccc tggcggaaga gatgggcgtt
     6241 ctgcaggaac gtatcacctc caccaaaact ggttctatca cctccgtaca ggcggtatac
     6301 gtacctgcgg atgacttgac tgacccgtct ccggcaacca cctttgcgca ccttgacgca
     6361 accgtggtac tgagccgtca gatcgcgtct ctgggtatct acccggccgt tgacccgctg
     6421 gactccacca gccgtcagct ggacccgctg gtggttggtc aggaacacta cgacaccgcg
     6481 cgtggcgttc agtccatcct gcaacgttat caggaactga aagacatcat cgccatcctg
     6541 ggtatggatg aactgtctga agaagacaaa ctggtggtag cgcgtgctcg taagatccag
     6601 cgcttcctgt cccagccgtt cttcgtggca gaagtattca ccggttctcc gggtaaatac
     6661 gtctccctga aagacaccat ccgtggcttt aaaggcatca tggaaggcga atacgatcac
     6721 ctgccggagc aggcgttcta catggtcggt tccatcgaag aagctgtgga aaaagccaaa
     6781 aaactttaac gccttaatcg gagggtgata tggcaatgac ttaccacctg gacgtcgtca
     6841 gcgcagagca acaaatgttc tctggtctgg tcgagaaaat ccaggtaacg ggtagcgaag
     6901 gtgaactggg gatctaccct ggccacgcac cgctgctcac cgccattaag cctggtatga
     6961 ttcgcatcgt gaaacagcac ggtcacgaag agtttatcta tctgtctggc ggcattcttg
     7021 aagtgcagcc tggcaacgtg accgttctgg ccgacaccgc aattcgcggc caggatctcg
     7081 acgaagcgcg agccatggaa gcgaaacgta aggctgaaga gcacattagc agctctcacg
     7141 gcgacgtaga ttacgctcag gcgtctgcgg aactggccaa agcgatcgcg cagctgcgcg
     7201 ttatcgagtt gaccaaaaaa gcgatgtaac accggcttga aaagcacaaa agccagtctg
     7261 gaaacaggct ggcttttttt acgcgttgac ccgtcctgaa gagcgttcac atagatcctg
     7321 ctgatataaa acacccctgt tttcccgttt attcattgat cgaaataaga gcaaaaacat
     7381 ccacctgacg cttaaatgaa ggtactgcct taattttctg cagataaaag gcgtgacgat
     7441 ggtcgaaaat ggcactttcg tcagcgggga taatccgtta ttgaacaatt tatcctctgt
     7501 ccatttcacg atgaaaaaaa tgtagttttt tcaaggtgaa gcggtttaaa ttcgttctca
     7561 aattacagtc aggacgcgta tgttgaataa tgctatgagc gtagtgatcc ttgccgcagg
     7621 caaaggcacg cgcatgtatt ccgatcttcc gaaagtgctg catacccttg ccgggaaagc
     7681 gatggttcag catgtcattg atgctgcgaa tgaattaggc gcagcgcacg ttcacctggt
     7741 gtacggtcac ggcggcgatc tgcttaaaca ggcgctgaaa gacgacaacc ttaactgggt
     7801 gcttcaggca gagcagctgg gtacgggtca tgcaatgcag caggccgcac ctttctttgc
     7861 cgatgatgaa gacattttaa tgctctacgg cgacgtgccg ctgatctctg tcgaaacact
     7921 ccagcgcctg cgtgatgcta aaccccaggg tggcattggt ctgctgacgg tgaaactgga
     7981 tgatccgacc ggttatggac gtatcacccg tgaaaatggc aaagttaccg gcattgttga
     8041 gcacaaagat gccaccgacg agcagcgtca gattcaggag atcaacaccg gcattctgat
     8101 tgccaacggc gcagatatga aacgctggct ggcgaagctg accaacaata atgctcaggg
     8161 cgaatactac atcaccgaca ttattgcgct ggcgtatcag gaagggcgtg aaatcgtcgc
     8221 cgttcatccg caacgtttaa gcgaagtaga aggtgtgaat aaccgcctgc aactctcccg
     8281 tctggagcgc gtttaccagt ccgaacaggc tgaaaaactg ctgttagcag gcgttatgct
     8341 gcgcgatccg gcgcgttttg atctgcgcgg tacgcttact cacgggcgcg atgttgaaat
     8401 tgatactaac gttatcatcg agggcaacgt gactctcggt catcgcgtga aaatcggcac
     8461 cggttgcgtg attaaaaaca gcgtgattgg cgatgattgc gaaatcagtc cgtataccgt
     8521 tgtggaagac gcgaatctgg cagcggcctg tactattggc ccgtttgccc gtctgcgtcc
     8581 tggtgctgag ttgctggaag gtgcacacgt tggtaacttc gttgagatga aaaaagcgcg
     8641 tctgggtaaa ggcacgaaag ccgggcatct gacttacctg ggcgatgcgg aaattggcga
     8701 taacgttaac atcggcgcgg gaaccattac ctgcaactac gatggtgcga ataaatttaa
     8761 gaccattatc ggcgacgatg tgtttgtcgg ttccgacact cagctggtgg ccccggtaac
     8821 agtaggcaaa ggcgcgacca ttgctgcggg tacaactgtg acgcgtaatg tcggcgaaaa
     8881 tgcattagct atcagccgtg tgccgcagac tcagaaagaa ggctggcgtc gtccggtaaa
     8941 gaaaaagtaa ttctggccgg gtaatccggt catctgggat gaggagataa cataatctcc
     9001 ctcccacaag cagtaactat aaaaataacc ccactctcta caaggctcgg ggcgcccgaa
     9061 aaaacgggca tacaggttga ccgacaacga tataaatcgg aatcaaaaac tatgtgtgga
     9121 attgttggcg cgatcgcgca acgtgatgta gcagaaatcc ttcttgaagg tttacgtcgt
     9181 ctggaatacc gcggatatga ctctgccggt ctggccgttg ttgatgcgga aggtcatatg
     9241 actcgcctgc gtcgcctcgg taaagtccag atgctggctc aggcagcgga agaacatcct
     9301 ctgcatggcg gcaccggtat tgctcacact cgctgggcga cccacggtga accttcagaa
     9361 gctaacgcgc atccgcatgt ttctgaacac attgtggtgg tgcataatgg catcatcgaa
     9421 aaccatgaac cgctgcgtga agagttaaaa gcgcgtggct ataccttcgt ttctgaaacc
     9481 gacaccgaag tgattgccca tctggtgaac tgggagctga aacaaggcgg gactctgcgt
     9541 gaggctgttc tgcgtgctat cccgcagctg cgcggtgcgt acggtacagt gatcatggac
     9601 tcccgtcacc cggataccct gctagcggca cgttctggta gtccgctggt gattggcctg
     9661 ggtatgggcg aaaactttat cgcttctgac cagctggcgc tgttgccggt gacccgtcgc
     9721 tttatcttcc ttgaagaggg cgatattgcg gaaatcactc gccgttcggt aaacatcttc
     9781 gataaaactg gcgcggaagt aaaacgtcag gatatcgaat ccaatctgca atatgacgcg
     9841 ggcgataaag gtatttaccg tcactacatg cagaaagaga tctacgaaca gccgaacgcg
     9901 ataaaaaaca cccttaccgg gcgcatcagc cacggtcagg ttgatttaag cgagctggga
     9961 ccgaacgccg acgaactgct gtcgaaggtt gagcatattc agatcctcgc ctgtggtact
    10021 tcttataact ccggtatggt ttcccgctac tggtttgaat cgctggcagg tattccgtgc
    10081 gacgtcgaaa tcgcctctga attccgctat cgtaaatctg ccgtgcgtcg taacagcctg
    10141 atgatcacct tgtcacagtc tggcgaaacc gcggataccc tggcgggcct gcgtctgtcg
    10201 aaagagctgg gttaccttgg ttcgctggca atctgtaacg ttccgggttc ttctctggtg
    10261 cgcgaatccg atctggcgct gatgaccaac gcgggtacgg aaatcggcgt ggcgtccact
    10321 aaagcattca ccactcagtt aactgtgctg ttgatgctgg tggcgaagct gtctcgcctg
    10381 aaaggtctgg atgcctccat tgaacatgac attgtgcatg gtctgcaggc gctgccgagc
    10441 cgtattgagc agatgctgtc tcaggataaa cgcattgaag ctctggcaga agatttctct
    10501 gacaaacatc acgcgctgtt cctgggccgt ggcgatcagt acccaatcgc gctggaaggc
    10561 gcattgaagc tgaaagagat ctcttacatt cacgctgaag cctacgctgc aggcgagctg
    10621 aaacacggcc cgctggcgct gattgacgcg gatatgccgg ttatcgtcgt tgcaccgaac
    10681 aacgaactgt tggaaaaact gaaatccaac attgaagaag ttcgcgcgcg tggcggtcaa
    10741 ttgtatgtct tcgccgatca ggatgcgggt tttgtaagta gcgataacat gcacatcatc
    10801 gagatgccgc atgtggaaga ggtgattgca ccgatcttct acaccgttcc gctgcagctg
    10861 ctggcttacc atgtcgcgct gatcaaaggc accgacgttg accagccgcg taacctggca
    10921 aaatcggtta cggttgagta atcactatca tagataaaaa agggctgctg tgggcggaca
    10981 ataaagtctt aaactgaaca aaatagatct aaactatgac aataaagtct taaactagac
    11041 agaatagttg taaactgaaa tcagtccagt tatgctgtga aaaagcatac tggacttttg
    11101 ttatggctaa agcaaactct tcattttctg aagtgcaaat tgcccgtcgt attaaagagg
    11161 ggcgtggcca agggcatggt aaagactata ttccatggct aacagtacaa gaagttcctt
    11221 cttcaggtcg ttcccaccgt atttattctc ataagacggg acgagtccat catttgctat
    11281 ctgacttaga gcttgctgtt tttctcagtc ttgagtggga gagcagcgtg ctagatatac
    11341 gcgagcagtt ccccttatta cctagtgata ccaggcagat tgcaatagat agtggtatta
    11401 agcatcctgt tattcgtggt gtagatcagg ttatgtctac tgatttttta gtggactgca
    11461 aagatggtcc ttttgagcag tttgctattc aagtcaaacc tgcagcagcc ttacaagacg
    11521 agcgtacctt agaaaaacta gaactagagc gtcgctattg gcagcaaaag caaattcctt
    11581 ggttcatttt tactgataaa gaaataaatc ccgtagtaaa agaaaatatt gaatggcttt
    11641 attcagtgaa aacagaagaa gtttctgcgg agcttttagc acaactatcc ccattggccc
    11701 atatcctgca agaaaaagga gatgaaaaca ttatcaatgt ctgtaagcag gttgatattg
    11761 cttatgattt ggagttaggc aaaacattga gtgagatacg agccttaacc gcaaatggtt
    11821 ttattaagtt caatatttat aagtctttca gggcaaataa gtgtgcagat ctctgtatta
    11881 gccaagtagt gaatatggag gagttgcgct atgtggcaaa ttaatgaggt tgtgctattt
    11941 gataatgatc cgtatcgcat tttggctata gaggatggcc aagttgtctg gatgcaaata
    12001 agcgctgata aaggagttcc acaagctagg gctgagttgt tgctaatgca gtatttagat
    12061 gaaggccgct tagttagaac tgatgaccct tatgtacatc ttgatttaga agagccgtct
    12121 gtagattctg tcagcttcca gaagcgcgag gaggattatc gaaaaattct tcctattatt
    12181 aatagtaagg atcgtttcga ccctaaagtc agaagcgaac tcgttgagca tgtggtccaa
    12241 gaacataagg ttactaaggc tacagtttat aagttgttac gccgttactg gcagcgtggt
    12301 caaacgccta atgcattaat tcctgactac aaaaacagcg gtgcaccagg ggaaagacgt
    12361 tcagcgacag gaacagcaaa gattggccga gccagagaat atggtaaggg tgaaggaacc
    12421 aaggtaacgc ccgagattga acgccttttt aggttgacca tagaaaagca cctgttaaat
    12481 caaaaaggta caaagaccac cgttgcctat agacgatttg tggacttgtt tgctcagtat
    12541 tttcctcgca ttccccaaga ggattaccca acactacgtc agtttcgtta tttttatgat
    12601 cgagaatacc ctaaagctca gcgcttaaag tctagagtta aagcaggggt atataaaaaa
    12661 gacgtacgac ccttaagtag tacagccact tctcaggcgt taggccctgg gagtcgttat
    12721 gagattgatg ccacgattgc tgatatttat ttagtggatc atcatgatcg ccaaaaaatc
    12781 ataggaagac caacgcttta cattgtgatt gatgtgttta gtcggatgat cacgggcttt
    12841 tatatcggct ttgaaaatcc gtcttatgtg gtggcgatgc aggcttttgt aaatgcttgc
    12901 tctgacaaaa cggccatttg tgcccagcat gatattgaga ttagtagctc agactggccg
    12961 tgtgtaggtt tgccagatgt gttgctagcg gaccgtggcg aattaatgag tcatcaggtc
    13021 gaagccttag tttctagttt taatgtgcga gtggaaagtg ctccacctag acgtggcgat
    13081 gctaaaggca tagtggaaag cacttttaga acactacaag ccgagtttaa gtcctttgca
    13141 cctggcattg tagagggcag tcggatcaaa agccatggtg aaacagacta taggttagat
    13201 gcatctctgt cggtatttga gttcacacaa attattttgc gtacgatctt attcagaaat
    13261 aaccatctgg tgatggataa atacgatcga gatgctgatt ttcctacaga tttaccgtct
    13321 attcctgtcc agctatggca atggggtatg cagcatcgta caggtagttt aagggctgtg
    13381 gagcaagagc agttgcgagt agcgttactg cctcgccgaa aggtctctat ttcttcattt
    13441 ggcgttaatt tgtggggttt gtattactcg gggtcagaga ttctgcgtga gggttggttg
    13501 cagcggagca ctgatatagc tagacctcaa catttagaag cggcttatga cccagtgctg
    13561 gttgatacga tttatttgtt tccgcaagtt ggcagccgtg tattttggcg ctgtaatctg
    13621 acggaacgta gtcggcagtt taaaggtctc tcattttggg aggtttggga tatacaagca
    13681 caagaaaaac acaataaagc caatgcgaag caggatgagt taactaaacg cagggagctt
    13741 gaggcgttta ttcagcaaac cattcagaaa gcgaataagt taacgcccag tactactgag
    13801 cccaaatcaa cacgcattaa gcagattaaa actaataaaa aagaagccgt gacctcggag
    13861 cgtaaaaaac gtgcggagca tttgaagcca agctcttcag gtgatgaggc taaagttatt
    13921 cctttcaacg cagtggaagc ggatgatcaa gaagattaca gcctacccac atacgtgcct
    13981 gaattatttc aggatccacc agaaaaggat gagtcatgag tgctacccgg attcaagcag
    14041 tttatcgtga tacgggggta gaggcttatc gtgataatcc ttttatcgag gccttaccac
    14101 cattacaaga gtcagtgaat agtgctgcat cactgaaatc ctctttacag cttacttcct
    14161 ctgacttgca aaagtcccgt gttatcagag ctcataccat ttgtcgtatt ccagatgact
    14221 attttcagcc attaggtacg catttgctac taagtgagcg tatttcggtc atgattcgag
    14281 gtggctacgt aggcagaaat cctaaaacag gagatttaca aaagcattta caaaatggtt
    14341 atgagcgtgt tcaaacggga gagttggaga catttcgctt tgaggaggca cgatctacgg
    14401 cacaaagctt attgttaatt ggttgttctg gtagtgggaa gacgacctct cttcatcgta
    14461 ttctagccac gtatcctcag gtgatttacc atcgtgaact caatgtagag caggtggtgt
    14521 atttgaaaat agactgctcg cataatggtt cgctaaaaga aatctgcttg aattttttca
    14581 gagcgttgga tcgagccttg ggctcgaact atgagcgtcg ttatggctta aaacgtcatg
    14641 gtatagaaac catgttggct ttgatgtcgc aaatagccaa tgcacatgct ttagggttgt
    14701 tggttattga tgaaattcag catttaagcc gctctcgttc gggtggatct caagagatgc
    14761 tgaacttttt tgtgacgatg gtgaatatta ttggcgtacc agtgatgttg attggtaccc
    14821 ctaaagcacg agagattttt gaggctgatt tgcggtctgc acgtagaggg gcagggtttg
    14881 gagctatatt ctgggatcct atacaacaaa cgcaacgtgg aaagcccaat caagagtgga
    14941 tcgcttttac ggataatctt tggcaattac agcttttaca acgcaaagat gcgctgttat
    15001 cggatgaggt ccgtgatgtg tggtatgagc taagccaagg agtgatggac attgtagtaa
    15061 aactttttgt actcgctcag ctccgtgcgc tagctttagg caatgagcgt attaccgctg
    15121 gtttattgcg gcaagtgtat caagatgagt taaagcctgt gcaccccatg ctagaggcat
    15181 tacgctcggg tatcccagaa cgcattgctc gttattctga tctagtcgtt cccgagattg
    15241 ataaacggtt aatccaactt cagctagata tcgcagcgat acaagaacaa acaccagaag
    15301 aaaaagccct tcaagagtta gataccgaag atcagcgtca tttatatctg atgctgaaag
    15361 aggattacga ttcaagcctg ttaattccca ctattaaaaa agcgtttagc cagaatccaa
    15421 cgatgacaag acaaaagtta ctgcctcttg ttttgcagtg gttgatggaa ggcgaaacgg
    15481 tagtgtcaga actagaaaag ccctccaaga gtaaaaaggt ttcggctata aaggtagtca
    15541 agcccagcga ctgggatagc ttgcctgata cggatttacg ttatatctat tcacaacgcc
    15601 aacctgaaaa aaccatgcat gaacggttaa aagggaaagg ggtaatagtg gatatggcga
    15661 gcttatttaa acaagcaggt tagccatgag aaactttcct gttccgtact cgaatgagct
    15721 gatttatagc actattgcac gggcaggcgt ttatcaaggg attgttagtc ctaagcagct
    15781 gttggatgag gtgtatggca accgcaaggt ggtcgctacc ttaggtctgc cctcgcattt
    15841 aggtgtgata gcaagacatc tacatcaaac aggacgttac gctgttcagc agcttattta
    15901 tgagcatacc ttattccctt tatatgctcc gtttgtaggc aaggagcgcc gagacgaagc
    15961 tattcggtta atggagtacc aagcgcaagg tgcggtgcat ttaatgctag gagtcgctgc
    16021 ttctagagtt aagagcgata accgctttag atactgccct gattgcgttg ctcttcagct
    16081 aaataggtat ggggaagcct tttggcaacg agattggtat ttgcccgctt tgccatattg
    16141 tccaaaacac ggtgctttag tcttctttga tagagctgta gatgatcacc gacatcaatt
    16201 ttgggctttg ggtcatactg agctgctttc agactacccc aaagactccc tatctcaatt
    16261 aacagcacta gctgcttata tagcccctct gttagatgct ccacgagcgc aagagctttc
    16321 cccaagcctt gagcagtgga cgctgtttta tcagcgctta gcgcaggatc tagggctaac
    16381 caaaagcaag cacattcgtc atgacttggt ggcggagaga gtgaggcaga cttttagtga
    16441 tgaggcacta gagaaactgg atttaaagtt ggcagagaac aaggacacgt gttggctgaa
    16501 aagtatattc cgtaagcata gaaaagcctt tagttattta cagcatagta ttgtgtggca
    16561 agccttattg ccaaaactaa cggttataga agcgctacag caggcaagtg ctcttactga
    16621 gcactctata acgacaagac ctgttagcca gtctgtgcaa cctaactctg aagatttatc
    16681 tgttaagcat aaagactggc agcaactagt gcataaatac caaggaatta aggcggcaag
    16741 acagtcttta gagggtgggg tgctatacgc ttggctttac cgacatgaca gggattggct
    16801 agttcactgg aatcaacagc atcaacaaga gcgtctggca cccgccccta gagttgattg
    16861 gaaccaaaga gatcgaattg ctgtacgaca actattaaga atcataaagc gtctagatag
    16921 tagccttgat cacccaagag cgacatcgag ctggctgtta aagcaaactc ctaacggaac
    16981 ctctcttgca aaaaatctac agaaactgcc tttggtagcg ctttgcttaa agcgttactc
    17041 agagagtgtg gaagattatc aaattagacg gattagccaa gcttttatta agcttaaaca
    17101 ggaagatgtt gagcttaggc gctggcgatt attaagaagt gcaacgttat ctaaagagcg
    17161 gataactgag gaagcacaaa gattcttgga aatggtttat ggggaagagt gagtggttag
    17221 gctagctaca tttaatgaca atgtgcaggt tgtacatatt ggtcatttat tccgtaactc
    17281 gggtcataag gagtggcgta tttttgtttg gtttaatcca atgcaagaac ggaaatggac
    17341 tcgatttact catttgcctt tattaagtcg agctaaggtg gttaacagta caacaaagca
    17401 aataaataag gcggatcgtg tgattgagtt tgaagcatcg gatcttcaac gagccaaaat
    17461 aatcgatttt cctaatctct cgtcctttgc ttccgtacgc aacaaggatg gagcgcagag
    17521 ttcatttatt tacgaagctg aaacaccata tagcaagact cgttatcaca tcccacagtt
    17581 agagctagct cggtcattat ttttaattaa ctcctatttc tgtcgaagct gtttgagcag
    17641 taccgcttta cagcaagagt tcgacgttca gtatgaggtt gagcgagatc atttagagat
    17701 aaggatctta cccagttcat cgtttcctaa aggggcgtta gagctgtcgg ccgtagtgca
    17761 gcttttggtt tggttgtttt cggatcaaga tgttatggat tcgtatgaaa gtatttttag
    17821 gcactatcaa caaaatagag aaattaagaa cggcgttgaa agctggtgct ttagctttga
    17881 ccctccgccc atgcagggtt ggaaattaca tgtaaaagga cgttcttcta acgaggataa
    17941 ggattattta gttgaggaaa tagtaggttt agaaatcaac gctatgcttc ctagcacaac
    18001 agctattagc catgcctctt ttcaggaaaa ggaggcaggt gatggtagta cgcagcacat
    18061 agcggtttca acagagtcag ttgttgatga tgagcatcta cagttggacg atgaggaaac
    18121 agccaatata gacacagaca cacgagtcat agaggctgag ccgacatgga taagttttag
    18181 tagacctagt cgaattgaaa aatctcgcag ggcaagaaaa agtagccaaa ctattttaga
    18241 aaaagaagaa gcaacaacaa gtgaaaatag taatttggtt agtactgatg agccacactt
    18301 aggtggtgtc ctagcagcgg cagatgtggg tgggaagcag gatgcaacca attacaactc
    18361 tatttttgct aatcgatttg ctgcttttga tgagctactt tcaattctaa aaactaaatt
    18421 tgcatgtcgg gtgctttttg aagaaacctt ggttttgcca aaagttgggc gtagccgatt
    18481 acatctgtgt aaagatggct caccaagagt gattaaagcc gttggggtgc aacgtaatgg
    18541 cagtgaattt gtattgctag aggtggatgc atcggatggg gtgaaaatgc tttctaccaa
    18601 agtgttgagt ggcgttgata gcgaaacatg gcggaatgat tttgaaaaga tacggcgtgg
    18661 agtggtgaag agctcattga attggccaaa tagtttgttt gatcaattat atggacaaga
    18721 cgggcataga ggggtgaatc atccaaaggg gttgggggag ctgcaagtat cgagagagga
    18781 tatggaaggg tgggctgaga gagtggttag agagcaattt acgcattaaa ggaatgactg
    18841 aaagagcctg taaacccttt tgtgtaagtg cttttgccgg tcagttaaag gtggccattt
    18901 aaacggtcac caaattcgat cataaaacgg ttcatggccg gcttccagtt gcggatcggc
    18961 atcgtccatt tcttggtcgc cgcctggata gccaggtaca ccaccttcat cgctgattcg
    19021 tccgtaggga acaccttgag ttcatattaa tggaattttc tacaaatagc ctccgtggtt
    19081 ttgagggggg attacagacg atccatagta gtaatccaat gagttcttga gcgcggcgac
    19141 atgtttggac gccttggcaa aaattagagc ctgcttgaag tgcaggcgag cacgtgcttg
    19201 gacgattgat ccattcgcgg tcaaaaactc aatcttggat gacagcgtgt caggaaatcc
    19261 aatatcgaag tcccgccccg tgtcaaagta gggcatttca tgatcaaagg acgaagcttc
    19321 ccaggccact tccctaatat tccagccgct agaaacaaga tttcgtacga cttcatcaac
    19381 ttttttgctt tccaacttgt cacaaagtgc ataggcaaga gctttggcgg cagacacgtg
    19441 cgtttcctct taaacatagg tcgaacggat acccgaggca aagcggagca ttagtcccaa
    19501 agcatctccg gtagatccgg aggctccata ctgaaggcat ggcatcttgg gcattgatat
    19561 ttgaaacttc tctctagata gcagggattt cttggccttg cctcatgctg atagcttgta
    19621 aatatcacac gatccttagc cccacaaagt gagcatactc cgtagctttc atttatagaa
    19681 atcgtcgtta gattgtcgca gcatgtgcac gttccgacta tttgctcagg aagcatc
//
LOCUS       DAZPAB010000064        19698 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZPAB010000064 DAZPAB010000000
VERSION     DAZPAB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.064"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACHUPG_001993"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="ACHUPG_001993"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR0861619.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACHUPG_001994"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="ACHUPG_001994"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR0861620.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACHUPG_001995"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="ACHUPG_001995"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR0861621.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="ACHUPG_001996"
     CDS             6038..6658
                     /locus_tag="ACHUPG_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR0861622.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="ACHUPG_001997"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="ACHUPG_001997"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR0861623.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="ACHUPG_001998"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="ACHUPG_001998"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR0861624.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="ACHUPG_001999"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="ACHUPG_001999"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR0861625.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="ACHUPG_002000"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="ACHUPG_002000"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR0861626.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10068..11069)
                     /locus_tag="ACHUPG_002001"
     CDS             complement(10068..11069)
                     /locus_tag="ACHUPG_002001"
                     /inference="COORDINATES: protein motif:HMM:NF007315.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0861627.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     ELLYRSMKFVSARNFWRHGASNGWASPMKAIRSCRSTRNHSGSFSRFLLNFVQIFVS"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="ACHUPG_002002"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="ACHUPG_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="ACHUPG_002003"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="ACHUPG_002003"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR0861628.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="ACHUPG_002004"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="ACHUPG_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR0861629.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="ACHUPG_002005"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="ACHUPG_002005"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR0861630.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="ACHUPG_002006"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="ACHUPG_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR0861631.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="ACHUPG_002007"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="ACHUPG_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR0861632.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..19633
                     /gene="rpoD"
                     /locus_tag="ACHUPG_002008"
     CDS             17792..19633
                     /gene="rpoD"
                     /locus_tag="ACHUPG_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR0861633.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4683 a         5506 c         5031 g         4478 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtatcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcaccgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    19261 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    19321 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    19381 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    19441 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    19501 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    19561 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    19621 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc
    19681 tgcacaaacg ccaccttt
//
LOCUS       DAZPAB010000065        19061 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZPAB010000065 DAZPAB010000000
VERSION     DAZPAB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.065"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..1025)
                     /locus_tag="ACHUPG_002009"
     CDS             complement(<1..1025)
                     /locus_tag="ACHUPG_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0861634.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1127..2107)
                     /locus_tag="ACHUPG_002010"
     CDS             complement(1127..2107)
                     /locus_tag="ACHUPG_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFR0861635.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2115..2825)
                     /locus_tag="ACHUPG_002011"
     CDS             complement(2115..2825)
                     /locus_tag="ACHUPG_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR0861636.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3042..4865)
                     /gene="typA"
                     /locus_tag="ACHUPG_002012"
     CDS             complement(3042..4865)
                     /gene="typA"
                     /locus_tag="ACHUPG_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR0861637.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5238..6647
                     /gene="glnA"
                     /locus_tag="ACHUPG_002013"
     CDS             5238..6647
                     /gene="glnA"
                     /locus_tag="ACHUPG_002013"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR0861638.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6820..7869
                     /gene="glnL"
                     /locus_tag="ACHUPG_002014"
     CDS             6820..7869
                     /gene="glnL"
                     /locus_tag="ACHUPG_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR0861639.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7881..9290
                     /gene="glnG"
                     /locus_tag="ACHUPG_002015"
     CDS             7881..9290
                     /gene="glnG"
                     /locus_tag="ACHUPG_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR0861640.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9402..9512
                     /locus_tag="ACHUPG_002016"
     CDS             9402..9512
                     /locus_tag="ACHUPG_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR0861641.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="ACHUPG_002017"
     CDS             complement(9741..11114)
                     /gene="hemN"
                     /locus_tag="ACHUPG_002017"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR0861642.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="ACHUPG_002018"
     CDS             complement(11303..11812)
                     /gene="yihI"
                     /locus_tag="ACHUPG_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR0861643.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(11931..12097)
                     /locus_tag="ACHUPG_002019"
                     /pseudo
     CDS             complement(11931..12097)
                     /locus_tag="ACHUPG_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12394..13026
                     /gene="yihA"
                     /locus_tag="ACHUPG_002020"
     CDS             12394..13026
                     /gene="yihA"
                     /locus_tag="ACHUPG_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR0861644.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13408..16194)
                     /gene="polA"
                     /locus_tag="ACHUPG_002021"
     CDS             complement(13408..16194)
                     /gene="polA"
                     /locus_tag="ACHUPG_002021"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR0861645.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16558..17490
                     /locus_tag="ACHUPG_002022"
     CDS             16558..17490
                     /locus_tag="ACHUPG_002022"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR0861646.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17531..18961)
                     /locus_tag="ACHUPG_002023"
     CDS             complement(17531..18961)
                     /locus_tag="ACHUPG_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR0861647.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLAYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         4663 a         4807 c         4564 g         5027 t
ORIGIN      
        1 gcttcgccga taggtgcaaa aaatacggct cgctgtgtaa agactatcgc gccaaagccc
       61 agcgtacatg ccatccctaa atagaccggc atactttcgt gcggcagcat aatcaacagt
      121 acgagcgcag cggtactgat gataaaggtg tagcatagat atttactcgg cgatttcagg
      181 atcttatctg aaatcatgcc accaatcggt ccaccaatca ttttcagaca gtattggtta
      241 atgatgccgt aagcccccac cagcgcaacg ggcaatagat agatgttttt caggaatgga
      301 atgaagaatg tcaggccaca gtaaaccgcg taaacgaaga agacgttgaa agcgataagc
      361 cagatggttt tgtctctcag cactgaggtc atactggtgt tttgcgatgc tccgtcttct
      421 tttttcacct caacggacgg tgcctcttct ttgtcattca ggacaaagaa aataataatg
      481 cctacggcaa tcacaataag ggaatagaac cagatgcctg ctttaaaacc taataagcca
      541 ctgccaaacc aggtaaatac tgccaacgca gaaaatgcca ccacggtatc gacaatgcca
      601 cgccctgttt cgaagaagcc aaacaaccga ccttgttgtt cgctattgcc caatcgactt
      661 accgacttga gcaagaccgg ccagttcatc atgtcgcaag taacgccaaa tagcgcccag
      721 acaaagagga tgccccaata ccccggcatt gtcgtcaaat aaacacccag taatcctgtc
      781 gcaatgagtg agaatgacat ggtgtatctg cgcggtagtt tatcggcaaa ataaatagac
      841 agaaaaaagc ccactgtggt gacaaatgag tttaccgaca tagcattacc aatttgacca
      901 ttggtcaaat ggaaatattc ctgcatcggg atataaaacg catctttcag cgacggtaat
      961 ttataaattg taccgccaca cagtgttaat agactaaata acgcccattt ctttttcgtg
     1021 agcataacat cccctaagcg atttttaaaa tcactgtttt aaaggcaata agataccgac
     1081 cattcatatg gaatggtttt attaactgcg tatgagttaa aacttattag tggttcagaa
     1141 gatggtttgc ctcttgtttg atttgttgta gtacgttacg cacatgattt atctgattaa
     1201 ttgcatcttc tttttcttta cgtaaccgcg catccagtaa atgattttct ggtaggggtg
     1261 tttcactcat ctggcgataa gggatccacg gattatcatc ttcgtcttca aagcggaacg
     1321 ccgacgcata gtaatcaacc tcttcttcca ggccatatgc cgtcacctgc ggctcatcat
     1381 cgccaaggca gataaggtgc gttaataacg ctttgaatgg catatcaccc tgacctgaaa
     1441 tacaggcttt atgacccagg ccaccctgtt ctttaacgat caaggcatct ttaatatgga
     1501 cctgggtaat atgcggtgcc atcgttttta aggcgtcgat gggatgctca tttgcattga
     1561 tcatgttagc aaaatcaaac agtaaggaaa gagactccat ctcactttct ttgaccagcg
     1621 acaccagctc atgacttttt aaatcttcat gctgctcaat cgtaaaagtc aggccgctgt
     1681 cctgatacgt ttcccgtaca taggcaatgt cgttagcgat aatcgataat acgtcgcgca
     1741 aattaccttc ataacatgga taaaaacgta cggacgttgc cccagttttc aacgcgatgg
     1801 cgacggcttc gtcaatagat gccttatctg aagcgctggt ttcaatatga atatcaaggt
     1861 tcagacggcg ggctttatcc ccaaaggcag agagttcttt atcgtccata ttaccaagag
     1921 aaaaacgctc gccatccaga acatggattt tgacgccacg tagattattt tcactggcga
     1981 tatcaagtaa atcagcaggc aacactcttt ccagccgcat atttaaatga aaggcataag
     2041 catgtaaata aagaggaaga gtgtcgacac gttgtagaat ctttcttgcg ttatttattg
     2101 tcaccataaa acacctacag attaatgatg aggtctacgg cctcaaatcg ccaatattca
     2161 atatctatct ggacaatatt gccttgctgg tcggcgcgat gcttttcaat taagatcgct
     2221 ggcatacccg cagaaccgcc aagatacttg ctgatatcgc ccggcattct gacgggtttg
     2281 aaaatcaatt tttttactac cgtctctttt tggtagcact tttcccagac tgcagaaaat
     2341 gagtggtttt caagttgttc aataaaaccc ggagcaactt caggattaat atatgtttcg
     2401 tgatagaaaa ctttatgtcc ttccagcgcc ccccagccgg tgatgcgata gagttcagtt
     2461 gatggcgtca ctgcaatcag tggcgcgagc gtctcgggaa tatcgctggt acggtttttc
     2521 tcggtaaacc cccaagaagg ctctcgccct tgctcaattg cggcccgctg aaagctcgcc
     2581 gacagctccg gactgtaatt aaatcgcggc tgggtcacga accacccctt acgatcctta
     2641 cggaagattt tggattctgc ctcgaggttc aacaacgcct ggcgcagcgt catacgttta
     2701 atgcccagca gttctcccag ctctctttct gaagggagtt ttccacccgg cgtggtgatg
     2761 ccatctttca accaccgatc cagtttctct tttgcatttt ctacggtgga ttgattctcc
     2821 gccattgttt aatccttttg gtttaaacca gatgaagcat cttacgctta cgccaaaaaa
     2881 gaaagcaatt aacggtgtat gactttgcag ataattgtga caaagctctc tattgacgta
     2941 atcacagact tataagggag atactcgcat tggtgtgcgc tcacgacagg taaaaaaaaa
     3001 cctgccatcg ctggcaggtt ttttatgact aaaaaacgaa attaatcgtc tttcggtgcg
     3061 cggttggcgc ggcgacgatc gttttccgtc aggtgacgtt tacgaatacg gatagaggtc
     3121 ggagtcactt ctaccagttc gtcatcatcg atgaactcca gagcttgttc cagagtcatg
     3181 cggataggcg gaaccagaac aacagcttcg tcagtaccgg aagcacgcat gttggtcagt
     3241 ttcttaccgg tcaggcagtt tacagtcagg tcgttagagc ggctatgaat accgataatc
     3301 tgaccttcgt aaacttctgc accgtgaccg aggaacagct taccgcgatc ctgcagaccg
     3361 aacagcgcga acgcgacagc tttaccctga ccgttagaga tcagtacacc gttctgacgc
     3421 tgacccactt cgcccggacg tacgtcgtcg tagtggctga aggtggagta cagcagacca
     3481 gtaccggaag tcatggtcat gaactcagaa cggaagccaa tcagaccacg gcttgggatc
     3541 acgtagtcga gacgtacgcg gcctttaccg tctggattca tgtttttcag gtcgccttta
     3601 cgttcgccca gcgcctgcat cacagaaccc tgatgctgtt cttcaacgtc cagcgtcacg
     3661 ttttcatacg gctcttgttt acgaccgtct atttcacgga agattacttt cggacgggat
     3721 accgccagtt cgaaaccttc acgacgcatg ttttcgataa gaacagagag gtgcagttca
     3781 ccacgaccag aaacgcggaa cgcatcggcg tcttcggttt cttctacgcg cagcgcaacg
     3841 ttgtgtacca gttctttgtt cagacgatcg aggatctgac gagacgttac gaacttacct
     3901 tctttaccgc agaacggcga ggtgttaacg cagaagaaca tagaaacggt cggctcatca
     3961 acggagagtg ccggcagcgc ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc
     4021 agttcgccca ggcccgtgat cgccacgata tcgccagctt ccgccagatc ggtttcgata
     4081 cgttccagac cgaggtggcc cagcacttta ccgactttcg cgttacgggt tttgccttcg
     4141 ctatcgatga tagtaacctg ctggttcggc ttcactttac cacgcttgat gcggccaatg
     4201 ccgataacgc caacatagct gttgtaatcg agctgagaaa tctgcatctg gaacggaccg
     4261 tcaaggtcaa cgtccggcgc aggaacgtgg tcaacaatcg cctgatacag cggggtcatg
     4321 tcttccgcca tatcttcgtg gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg
     4381 atcgggaagt ccagctgctc gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga
     4441 tccacaaccc aatcaggacg cgcgccaggg cggtcaactt tgttgataac aacaatcggc
     4501 ttcaggccgt aagcaaacgc ttttttggtt acgaagcgcg tttgcggcat cgggccgtca
     4561 aatgcgtcaa ccaccagcag cactgagtct accatggaca ttacacgttc aacttcacca
     4621 ccgaagtcgg cgtgccccgg ggtatcaacg atgttgatac ggtaatcatt ccatttgata
     4681 gcggtgtttt tcgcgaggat ggtaatccca cgctctttct ccaaatcgtt ggagtccatc
     4741 acgcgctctt gggtttcggc acgagagtcg aacgtaccgg attgttggag cagcttgtct
     4801 accagggtgg ttttaccatg gtctacgtgc gcgatgatgg cgatattacg caatttttcg
     4861 atcacaactt tgcctcaggc attagaaata gcgcgttatt gtacacggat taatcgcact
     4921 acaaaacagg atcacaaaca tcctccgcaa acaagtattg cagagtccct ttgtgatcgc
     4981 tttcacggag cataaaaagg gttatccaaa ggtcattgca ccaatatggt gcttaatgtt
     5041 tccattgaag cactatattg gtgcaacatt cacatcatgg tgcagccctt ttgcacgatg
     5101 ttaagcatga taacgccttt taggggcaat ttaaaagttg gcacagattt cgctttatct
     5161 tttttacggc gacacggcca aaataattgc agatttcgtt accacgacga caatgaccaa
     5221 tccaggagag ttaaagtatg tccgctgaac acgtactgac gatgctgaac gagcacgaag
     5281 tgaagtttgt tgatttgcgc ttcaccgata ccaaaggtaa agaacaacac gtcactatcc
     5341 ctgctcatca ggtgaatgct gaattcttcg aagaaggcaa aatgtttgac ggctcctcga
     5401 ttggcggctg gaaaggcatt aacgaatccg acatggtgct gatgccagac gcatccactg
     5461 cagtgattga cccgttcttc gccgactcca ccctgattat ccgttgcgac atccttgaac
     5521 ctggcaccct gcaaggctat gaccgtgacc cgcgctctat tgcgaagcgc gccgaagatt
     5581 acctgcgttc cactggcatt gccgacaccg tactgttcgg gccagaacct gaattcttcc
     5641 tgttcgatga catccgtttc ggatcatcta tctccggttc ccacgttgct atcgacgata
     5701 tcgaaggcgc atggaactcc tccacccaat acgaaggtgg taacaaaggt caccgtccgg
     5761 cagtgaaagg cggttacttc ccggttccgc cggtagactc tgctcaggat attcgttctg
     5821 aaatgtgtct ggtgatggaa cagatgggtc tggtggttga agcccatcac cacgaagtag
     5881 cgactgctgg tcagaacgaa gtggctaccc gcttcaatac catgaccaaa aaagctgacg
     5941 aaattcagat ctacaaatat gtcgtgcata acgtagcgca ccgcttcggt aaaaccgcga
     6001 cctttatgcc aaaaccgatg ttcggtgata acggctccgg tatgcactgc cacatgtctc
     6061 tgtctaaaaa cggcgttaac ctgttcgcag gcgacaaata cgcaggtctg tctgagcagg
     6121 cgctgtacta cattggcggc gtaatcaaac acgctaaagc gattaacgcc ctggcaaacc
     6181 cgaccaccaa ctcttataag cgtctggtcc cgggctacga agcaccggta atgctggctt
     6241 actctgcgcg taaccgttct gcgtctatcc gtattccggt ggtttcttct ccgaaagcac
     6301 gtcgtatcga agtacgtttc ccagatccgg cagctaaccc gtacctgtgc tttgctgccc
     6361 tgctgatggc cggtcttgat ggtatcaaga acaagatcca tccgggcgaa gccatggaca
     6421 aaaacctgta tgacctgccg ccagaagaag cgaaagagat cccacaggtt gcaggctctc
     6481 tggaagaagc actgaacgaa ctggatctgg accgcgagtt cctgaaagcc ggtggcgtgt
     6541 tcacggatga agcgatcgat gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc
     6601 gtatgactcc gcatccggta gagtttgagc tgtactacag cgtctaatag ttgaagttgt
     6661 actacccggc gcacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca
     6721 tgggcttttt tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata
     6781 atgcactaaa atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc
     6841 gatgctgggc agatcctcaa ctcgctgatt aacagtattt tgttaattga tgacaatctg
     6901 gcgatccatt acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg
     6961 tttggtacgc cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa
     7021 gaaagtctgg aggcggggca aggctttacc gataacgaag tgacgctggt catcgatggt
     7081 cgctcgcata tcctttctgt gacggcccag cgtatgccgg acggcatgat cctgctggag
     7141 atggcaccga tggataacca gcgccgctta agccaggaac aactacaaca tgctcaacaa
     7201 gtagctgccc gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt
     7261 ttacgtggcg cggcgcagtt actcagcaaa gcgttacctg acccatcact actcgaatat
     7321 accaaagtga ttatcgaaca ggcggaccgg ctgcgaaatt tggtcgaccg tctgttgggg
     7381 ccgcagctgc ccggtacgcg cgttaccgaa agtattcaca aagtggctga acgcgtggta
     7441 acgctggtgt cgatggaact gccggacaac gtgcggttga ttcgtgatta cgatcccagc
     7501 ctgccggaac tggcgcacga cccggatcaa attgaacagg tcttgctgaa tattgtgcgc
     7561 aatgcgctac aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac ccgcaccgcg
     7621 tttcaactga ccttacacgg cgagcgctac cggctggcgg cgcggattga tgtggaagat
     7681 aacgggccgg gcattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc
     7741 cgcgaaggtg gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca
     7801 ggcaaaattg aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc
     7861 aggaaataaa ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt
     7921 ccatccgttg ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acgacatttg
     7981 agaacggcgc ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag
     8041 atatccgtat gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc
     8101 caatgcttcc ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct
     8161 atcaacaagg ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gcagtggcgc
     8221 tggttgagcg cgctatcagt cattaccagg aacagcagca gccgcgtaat attcagctta
     8281 acggcccaac gaccgatatc atcggcgaag cgcaagccat gcaggacgtg ttccgtatta
     8341 tcggtcggct ttcgcgttct tctattagcg tgctgattaa cggcgaatcc ggcaccggta
     8401 aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg caccaaagcg ccgtttatcg
     8461 cgctgaatat ggcagctatc ccaaaagatt tgatcgaatc agaactgttt ggccacgaga
     8521 aaggcgcgtt tactggcgcg aataccattc gtcaggggcg ttttgaacag gccgatggcg
     8581 gcacgttatt tctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc
     8641 gcgtgctggc agacggtcag ttttatcgcg ttggcggcta tgcgccggtg aaagtggatg
     8701 tgcggattat cgctgccact caccagaatc ttgaacagcg ggtgcaggaa ggtaagttcc
     8761 gtgaggatct gttccaccgc ctgaacgtta tccgcgttca tctgccgccg ctgcgcgaac
     8821 gtcgggaaga tattccccgt ctggcgcgcc attttttaca ggttgccgcg cgcgaactgg
     8881 gcgtagaagc gaagttgctg catccggaaa ccgaagctgc tctgacgcgt ctggcgtggc
     8941 caggcaacgt gcgccagctg gaaaacacct gccgctggct aacggtgatg gccgccgggc
     9001 aggaagtgtt gattcaggat ttgcccggcg aactgtttga atcaacggtt gcggagagta
     9061 cttcgcaaat gcaaccggac agttgggcga cacttttagc gcagtgggca gacagagcgc
     9121 tgcgttccgg tcatcaaaat ctgctttccg aagcgcagcc agagctggag cggacgttac
     9181 tgacgaccgc gttgcgacat acgcaggggc ataaacagga agcggcgcgg ctactcggct
     9241 ggggccgcaa cactctgacg cgtaagttaa aagagctggg gatggagtga ttcacggctt
     9301 gtgtgtaaag attgattatt gagcgcaaat tgctggtatt ttgcgcttta ctgttccgat
     9361 aagttcagta tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta
     9421 atctggtatc gagtggcgca gttgacagcc acacaccgca aactgccgtt gctgccgtgc
     9481 tgtgtgccgc gatgattggg ctgtttagct gaagctcgtt gtttatgccg gatgcggaga
     9541 aagtcgtagg cctgataaga cgcgaccagc gtcgcatcag gcatcatgct cagatgcctg
     9601 atgcggcgta aacgccttat ccggcctaca aaagcaataa tttcaacagg ttttgttgcc
     9661 cagcgcatca ggcaatcttg ttctcagcac attgatggcg ctttcgtttt ctactttgta
     9721 aacgaagcgc cattcactct ttaaatcacc cgtgagaact gctgcatccg cgctttctgg
     9781 cgcagatagg tatcaaagca catgcaaatg ttgcggatca gcaagcgacc tttcgccgtc
     9841 acctgtattc ccttctcatc cacatccacc agcccatctt ttgctaacgg ggcgagcagc
     9901 ttgagatctt ccgcaaagta atcagcgaag tgcaaatccc actgtttctc aagaggggcg
     9961 taatccagac ggaagttgca gatgagcgac ttaatcacat cgcggcgaat acagtcatca
    10021 cgcgttagcg caataccacg ccacagcgca ttgccttgtt catacacttg ctgatagtac
    10081 tgcttcaact ctttctggtt ctgcgcgtag cagtcgccaa tcatgctgat ggcggaaacg
    10141 cccatcccca gcagatcggt atcgccctga gtggtgtagc cctggaagtt acgatgcagc
    10201 acgccttcac gctgggccac cgccagctcg tcatcaggac gggcaaagtg atccataccg
    10261 ataaactgat agcccgattg cgtcaggaag gcgatggttt cctgcaggat atcgagtttt
    10321 tgctgcggac tcggcaggtc agcatctttg attttgcgct gagcagcaaa aatggtcggc
    10381 agatgcgcgt agttaaaaac gctcagacga tcggggttca gctccgccac acgtttcagg
    10441 gtaaaggcga aactctccgg cgtctgtttc ggcaggccgt aaatcaggtc gatgttggtg
    10501 gaggtaaaac caatctcacg cgcatggtta agcagtgcaa agatgaactc ttcatcctgc
    10561 tcgcggttaa ccagacgttg cacttctttg ttgaagtcct gcacgcccat gctcaggcga
    10621 ttaaagcctt cggcgcgtaa atgatcgagt acatccagtt cgatttcccg cggatcgact
    10681 tcgatcgaaa tctccgcatc ggtattgaaa tggaagtttt cgcgcagcag cttcatcaga
    10741 cggctgattt gcgctttatt cagatacgtc ggcgttccgc cgccccagtg caattggctg
    10801 acgtgacgcc cggcgaacag cggtgcacga tggacgattt cttgctccag cgcgtccaga
    10861 tactgatcgg ccttgtgctg ctggcgagta acaatcttat tgcaaccgca gaagtaacaa
    10921 agcttatggc agaacgggat atgtacgtag agagataatg gacgctcagg atagcgcgcc
    10981 acggcttgta aaaacgcctg ttcgccgaag tcttctgaaa actccagcgc ggtcgggtac
    11041 gaggtgtatc gtggcccgga atagttatat ttctggatca gggccagatc ccagtcgatt
    11101 tgctgtacag acatgctcac tccttccgat ggcgtctctg gcggctacgg cgtaccggcg
    11161 gatgcccgtt tcgggtcatc agccggttgc gcagccactt ttgtcgccgc gacaaccgct
    11221 gtagtttaac gaataaccac accagataac atataaccgg aagggttatc agcagaacgg
    11281 gaaggcccac ggcggcaatc cgttagttgc cccgcagcag acgcatcatg tcttcttgct
    11341 tctcgtcttc ttcctcttct tcgtcatcgt cataagagag gccgagtttc tgcatcaact
    11401 catcaatacg atccagttta gcatccaccc aggattgctc ttcggcgctc agggtttcgc
    11461 ctgcttccag acgttccagc agcgcatcca gacactcatc cgtttccagt aattccaact
    11521 ccgcctgcgg tgaaagcata ggtttctcac tcttcggttt gtgctgtttg gtgacttttt
    11581 cagtcactcc caatggaata ggggttttac tgccaatacg tggatctttt ggtgcgttct
    11641 ggcctttgct gcctgacgtg gtgtttccgc ccgctgcgcg gctgcccggc gcatgaccac
    11701 gacgtttttt ctgacgctta cggtcacgag cttcctgatc cagctcctcg cgtgtttttc
    11761 gacgcgcttt tgcatgacct ttgctgcgtg agtttgaaga tgatggtttc ataatggttc
    11821 gtcttttagc cgttttattc agtatagatt tgcggcggaa tctaacagaa agcaagcaaa
    11881 gaaaaaaggc gacagattac tctgtcgcct tttttcctga ctcataaccc ttaacgggtc
    11941 ttacaatcct tgcaggccgc caacacaccc tgtttttcca ttagccggaa caatccgtgt
    12001 tgattccatt tccgtttaat tacgtctccg gacgtttgtc ttcctgacaa tcctctgtct
    12061 tcgcctcctg gcgctcctga ccctcatcct gagtcattgt tcctgttagc gtcctcgctc
    12121 tatgggtgct actttacgcc tttgctttaa acaaacaatc aaccgccatc agtatttgca
    12181 cattttcaga gttttatata aaagcaattc actgatttat atagcttatt tgacatgcat
    12241 cgcagaattt tctctgctat ttcgcataaa gactatcggc aatgtctcac aaagcgtatg
    12301 gcttttactt acaacgctca cgccgaaaaa tccgcctttt attatggttc aggtataatc
    12361 gccacaaagc tttgactgac ggagacgacc gctttgacta atttgaatta tcaacagacg
    12421 cattttgtga tgagtgcgcc tgatattcgc cacctacctt ccgataccgg aattgaagtg
    12481 gcttttgcag gccgttccaa cgcaggtaaa tccagcgcgc tgaacacgct gactaaccag
    12541 aaaagcctgg ctcgtacctc aaaaacccca gggcgcaccc agcttatcaa cctgtttgaa
    12601 gtggctgacg gcaagcgtct ggttgacttg cctgggtacg gttatgcgga agtcccggaa
    12661 gagatgaagc gcaaatggca gcgtgcgctc ggcgaatacc tcgaaaaacg tcagagcctg
    12721 caaggtctgg tggtgctaat ggatattcgc catccgctga aagatttgga tcagcagatg
    12781 attgagtggg cggtagacag caatatcgcc gttctggtgc tgctgaccaa agcggacaaa
    12841 ctggcaagcg gcgcacgtaa agcgctattg aatatggtgc gtgaagctgt actggcgttt
    12901 aacggtgatg tgcaggttga aacgttttct tcgttgaaga aacaaggcgt agacaagctg
    12961 aggcagaaac tggatacctg gtttagcgag atgcagcctg tagaagaaac gcaggacggc
    13021 gaataatttt cttgccttaa tgcttgtgcc ggatgtggcg tatccggccc gtaaattcaa
    13081 tagattacaa ttctaccgtt ggcctgataa acatagcgca tcaggcatta cggatctttt
    13141 ctttcgccca ataaaaaacg ccccagtcat tactgactgg ggcggctaaa atattcagcc
    13201 aaatccgatt acgtgaagta aaaggtctga aagatagaac atcttacctc tgtaccctac
    13261 gcgactaact ttactctttt ttgttcagtt cagaaagcac tttttgtaat tttttttcag
    13321 ttgttgcata ggaatttcta atagccatca caaaacgtga cagcttatgt tgcttactta
    13381 cgaaaaaagg gcatgttcag gcgaatctta gtgcgcctga tcccagtttt cgccactccc
    13441 cacttccacc agcaacggca catccagacg ggtacagttt tccatcagtt gatgaatctg
    13501 cttcgcgacg gcatcaacat catctttatg aacttcaaat accagttcat cgtgtacctg
    13561 catgatcata cgtacacgcg gttgctcagc ctgtaaccac gcatcaacgg caatcatcgc
    13621 ccgtttgata atgtcggcgg cggttccctg cattggcgcg ttaatggctg cacgttcagc
    13681 cgctgcacga cgagcaccat tgctggattt gatatccggc agatacagac ggcgtccgtc
    13741 cagcgtttca acgtagccca gctctttcgc ctgagcacgg gtgcgttcca tatactccag
    13801 cacgccaggg tagcgttcga agtaaaggtc catgtacttc tgcgcttctt tacgtggaat
    13861 gttcaattgc cgcgccagac cgaaagcact catgccataa atcagaccaa agttgatcgc
    13921 tttcgcgcta cggcgttgct cgctggtgac ggtttccagt ggcaaaccaa acacttctgc
    13981 cgccgttgcc cggtggatat cttttccttc cgcgaatgcg gtcagcaagc ctttatcacg
    14041 cgagagatgc gccataatgc gcagttcaat ctgcgagtag tccgctgaga caatcacata
    14101 atcctctggc gcaataaacg cctggcggat acgacgacct tcttcgttac gcaccggaat
    14161 gttttgcagg ttaggatcgg ttgacgataa acgtcccgtt gcagttactg cctggtgata
    14221 agaggtatgc acacgcccgg ttttcgggtt gatcatcagc ggcagcttgt cggtgtaagt
    14281 cgacttcagc ttcgccagac cacgatactc cagaatcact tttggcaacg gatagtccag
    14341 cgccagttct tccagtacct cttccgacgt tgacggcgcg ccacccggcg ttttcttcag
    14401 cggtttaata ccctgttttt caaacagaat ggtttgcaac tgcttggtgg aagaaaggtt
    14461 aaattcttca cctgcaattt catgcgcttt cttttccagc tcagccagac gcaaggtgag
    14521 ctcttcagaa tgattgtgca gcactttcgg atcgatcttc acaccgttac gttcaatgcg
    14581 tgaaagcacc ggcaccagcg gcatttcgat attctcgaag acgttcaacg gccctttgtg
    14641 tttctgcaga tccggccaca ttttcagatg caactgcaag gtgacatctg catcttcggc
    14701 ggcgtaacgt ccggcctctt cgagggcaat ctggttaaag gtcagttgat ttttgccttt
    14761 acccgcaatc tcttcaaaag tgatggtttt gtgcttcaac caacgttccg cgaggctgtc
    14821 catatcgtga cgcccggcaa cgctattgag aatgtaggac tccagcatgg tatcaaacgc
    14881 aatcccacgc agttcaatgc cgtaattcgc cagaataccg cgatcgtatt tcaggttttg
    14941 cccgaccttc agcgcctttt catcttccag cagcggtttt agcaactcga gtgcacgctc
    15001 gtgagagatt tgatcgggcg catcaagata atcatgagca accggaatat atgccgctac
    15061 gcctggctcg atagcaaaag aaagcccgac caggttagca gagatgttat caaggctgtc
    15121 ggtttcggta tcaaatgcaa ataccggcgc tttttccagc ttcgcaatcc acgctttcag
    15181 tgtttcttca tcaaggatag tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc
    15241 tggcgcttcg tctgcaacac tggtttcctg aggcttcgcg gctgtttttg cccctttggc
    15301 ctgtaaccat ttgcccactt cgacatcagc agtccagcgt ttgaactcat actttttgaa
    15361 cagccccaac aactcttccg ctgccggttg ctgcacttcc agttgttcac aggtcagctc
    15421 cagttcaaca tcggttttaa tcgtcgccaa ctggtatgag agataagcaa cttctttgtt
    15481 ttgctcgagc ttcgctgcca ttgttttcgc gccacggaag ctcaacccag caattttttc
    15541 tggctcggca tacagcgtat ccagcccgcc aagaccttgc agcaatgcct gcgcggtttt
    15601 ttcaccgacg cccggtacgc caggaatgtt atcagaggag tcacccatca gcgccaggaa
    15661 gtcgatgatc aattctggcg gcacgccgta cttattcacc acctcttccg gtccgaggat
    15721 ggtattcgtc atggtattga taagcgtaat atttggcgtc accagctgcg ccatatcttt
    15781 atcgccagtg ctaatcagca ccggacgccc ggctttttcg gcttcgcgcg ccagagtacc
    15841 gataacgtcg tccgcttcta cgccagaaac cgccagcagc ggcagtccca tcgctttaac
    15901 catcgcgtgc agaggttcga tttgtgctcg cagatcgtcc ggcattggcg ggcgatgtga
    15961 tttgtaatgt tcaaacagtt catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc
    16021 tgcatgcgtc ggtttatatt gcatgatcag actgcgcagc atgttgagga caccatacat
    16081 cgcaccggtc ggctcgcctg cgctgttagt cagcggggga aacgcgtgat atgcgcgata
    16141 aagataagat gaaccatcta caaggataag tggattttgg gggatctgaa ccataatgtc
    16201 cgtgcctgtt tatcagatta tgggtaaagg atgccacaga caggatgaaa acatgagttt
    16261 ttcaccgctt ttcgtgaaaa tcttttgtag atctgctgga tcgctcgcaa atcgtcatgt
    16321 ggataacttt gtgcataaat tatttacatc gcttttaatt tattatcatg ctttttacag
    16381 catgatgttt gtctttgttt ttcatgcagt taaaaataaa tccatcccta ataattgaga
    16441 taatgggatt ttttctaatg tggatagata tgaattattt ttctccttaa ggatcatccg
    16501 ttatttgggt cattttctgc taaaatctgc gccttgcgct tctttagtgc actgtctatg
    16561 gctaaccttt tgaataaatt cattatgacg agaatactcg ctgcgataac ccttttgctg
    16621 agtatcgtat tgaccatttt ggtcactatt ttttgttccg tcccgatcat cattgccggg
    16681 attgtaaaac tattgctacc tgtgccagtc atttggcgaa aggtttcgcg tttttgtgat
    16741 tttatgatgt attgctggtg tgaaggtctg gcggtattac tgcatctgaa cccacacctg
    16801 caatgggaag ttcacgggct ggaagggtta agtaagaaga actggtatct gcttatttgt
    16861 aaccaccgta gttgggcaga tattgtcgta ctgtgcgtgc tgtttcgtaa gcacattccg
    16921 atgaataaat atttcctcaa gcaacagctg gcctgggtgc catttcttgg cctggcgtgc
    16981 tgggcgctgg atatgccgtt tatgaagcgt tattcccgcg cttatttatt acgtcatccg
    17041 gagcgacgtg gtcaagatgt tgaaaccact cggcgttctt gtgaaaagtt tcgcctgcat
    17101 cccaccacca ttgtcaattt cgttgaaggc tcccgtttca cgcaagaaaa acatcagcaa
    17161 acccactcca cttttcaaaa cttgttgcca ccaaaggcgg caggcattgc gatggcgcta
    17221 aatgtactgg gtaaacaatt cgataaactg cttaatgtta cgctgtgtta tccggacaat
    17281 aaccgtcagc cgttcttcga tatgttaagc ggtaaactga cgcggattgt cgtacatgtg
    17341 gatttacagc ctattgccga tgagttacat ggcgactaca tcaatgacaa aagctttaag
    17401 cgccattttc agcaatggct gaactcattg tggcaggaaa aagaccggct tctgacgtct
    17461 ctcatgtcat cacagcgtca gaataagtaa gttttactcg cctctgaatg agcagaggcg
    17521 agtgagtatt ttaatgaaca aaacgtccgg cacgagacat aaattcttct tcgctcatct
    17581 cctgtccgtt aaaaacaact ttacccggtg tataacgcag ctgcagcgag gcggtattgt
    17641 tgtcaatcgt gattaactga aacatctgac ctaatgtcat catcccgctg atttgtttat
    17701 cagcctgctt ttgcgctttt tccgcatcca ttccttcaga taaattaagc tgttttgcgg
    17761 tttctgtgac gacattaagc ggtaacttca catcaaaatt gagcgatttg atatctttgt
    17821 ttgtggatga tgaagatttg gctgggtcgg caatactgat atccagattg gcatttagtt
    17881 cgccgagtgc gttcttccat cttaccggtt gtttaatggt cggctcactt ttttgtaata
    17941 acggtaaata ctctttgaac aatgcagcat tcacttcttg cagagcaact tcatcgtttg
    18001 ctaactcagg gtgtgcagca agctgcttct gcatcgcaat gttatactgg atgataaatt
    18061 ggcgcactgc tgagggatca atagattcag caatcaatga ccactcacca ctaccgagtt
    18121 gctgattccc ttttgttaac ttatcaagtt cgtaggccag attgacattg atataatctt
    18181 tgtctttaac gcgatccagc ctggtttttg caacgaaagc atcgatttgg gcaacgtctt
    18241 ccccgtgatt gataatgtta atctgattaa atttcgactc gctttcacca accggaaatt
    18301 tcttttcttc taatcgcgcc agagaatcaa tggtaaaact ttttgccgtt actttgttca
    18361 ttggtgataa ttgcagaaca atatcttcga ctttgccttc aatatccagg ttagacaatg
    18421 aactaccatc accattaaaa gtaaactggc cctcaccaaa agaaaatttt ccatactctg
    18481 caggggcaac atctaatgtc gtagagaatg agccaccaaa cgcgaatcgc aatgtcgctt
    18541 ccacgggcga tttatttttc gcggcgataa atagcggttg tgtcagttca ttattcacta
    18601 agttcacttt tgccgctgcc agtgctggga tgacattacc atgcattaac attgtgatgg
    18661 gcaacggacc gtgctcaaca tccacatcaa atacaacttt ctggcctggc ttgatattga
    18721 gatcgggtgc accgttatcg aatgtcattt gcatttgaaa acgggaatta aaaaagcctt
    18781 tgtcaaagtt tttataactt aacgtcatat cgtaggcatg ttcacctttc tttttcgcat
    18841 cgttaaaatc tttaataaac ttttcgatac caggctgaat ttgcgtacct gtgtaccatg
    18901 tgccaccacc ccagattacg gctaacgcaa caataacacc tgtagctgac ttacgtatca
    18961 tataaatatt ccctgataaa acagcaaaac tttgaatatc cacttatgct gaaataatgt
    19021 ccagcggcag gatgcattat cagaatgtat ttatatttat t
//
LOCUS       DAZPAB010000066        18668 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZPAB010000066 DAZPAB010000000
VERSION     DAZPAB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.066"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..186)
                     /locus_tag="ACHUPG_002024"
     CDS             complement(<1..186)
                     /locus_tag="ACHUPG_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR0861648.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYI"
     gene            complement(189..908)
                     /gene="sanA"
                     /locus_tag="ACHUPG_002025"
     CDS             complement(189..908)
                     /gene="sanA"
                     /locus_tag="ACHUPG_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR0861649.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(1058..1942)
                     /gene="cdd"
                     /locus_tag="ACHUPG_002026"
     CDS             complement(1058..1942)
                     /gene="cdd"
                     /locus_tag="ACHUPG_002026"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR0861650.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(2072..2767)
                     /locus_tag="ACHUPG_002027"
     CDS             complement(2072..2767)
                     /locus_tag="ACHUPG_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR0861651.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(2764..3162)
                     /locus_tag="ACHUPG_002028"
     CDS             complement(2764..3162)
                     /locus_tag="ACHUPG_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR0861652.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            3401..4348
                     /gene="dusC"
                     /locus_tag="ACHUPG_002029"
     CDS             3401..4348
                     /gene="dusC"
                     /locus_tag="ACHUPG_002029"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR0861653.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(4719..4802)
                     /gene="yohP"
                     /locus_tag="ACHUPG_002030"
     CDS             complement(4719..4802)
                     /gene="yohP"
                     /locus_tag="ACHUPG_002030"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFR0861654.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            5026..6462
                     /gene="mdtQ"
                     /locus_tag="ACHUPG_002031"
                     /pseudo
     CDS             5026..6462
                     /gene="mdtQ"
                     /locus_tag="ACHUPG_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            6530..7276
                     /locus_tag="ACHUPG_002032"
                     /pseudo
     CDS             6530..7276
                     /locus_tag="ACHUPG_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013097944.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(7393..7971)
                     /locus_tag="ACHUPG_002033"
     CDS             complement(7393..7971)
                     /locus_tag="ACHUPG_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0861655.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            8141..8728
                     /locus_tag="ACHUPG_002034"
     CDS             8141..8728
                     /locus_tag="ACHUPG_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR0861656.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            8902..9834
                     /gene="pbpG"
                     /locus_tag="ACHUPG_002035"
     CDS             8902..9834
                     /gene="pbpG"
                     /locus_tag="ACHUPG_002035"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR0861657.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9872..11587)
                     /gene="dld"
                     /locus_tag="ACHUPG_002036"
     CDS             complement(9872..11587)
                     /gene="dld"
                     /locus_tag="ACHUPG_002036"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR0861658.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            11783..14080
                     /gene="bglX"
                     /locus_tag="ACHUPG_002037"
     CDS             11783..14080
                     /gene="bglX"
                     /locus_tag="ACHUPG_002037"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR0861659.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            14332..15249
                     /gene="osmF"
                     /locus_tag="ACHUPG_002038"
     CDS             14332..15249
                     /gene="osmF"
                     /locus_tag="ACHUPG_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR0861660.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            15256..16413
                     /gene="yehY"
                     /locus_tag="ACHUPG_002039"
     CDS             15256..16413
                     /gene="yehY"
                     /locus_tag="ACHUPG_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFR0861661.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            16406..17332
                     /gene="yehX"
                     /locus_tag="ACHUPG_002040"
     CDS             16406..17332
                     /gene="yehX"
                     /locus_tag="ACHUPG_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR0861662.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            17337..18068
                     /gene="yehW"
                     /locus_tag="ACHUPG_002041"
     CDS             17337..18068
                     /gene="yehW"
                     /locus_tag="ACHUPG_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR0861663.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(18049..18156)
                     /locus_tag="ACHUPG_002042"
     CDS             complement(18049..18156)
                     /locus_tag="ACHUPG_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR0861664.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(18216..18668)
                     /locus_tag="ACHUPG_002043"
                     /pseudo
     CDS             complement(18216..18668)
                     /locus_tag="ACHUPG_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT         4448 a         4716 c         5145 g         4359 t
ORIGIN      
        1 aatatatgcc accatataag cgaagaagag tccgggattt tttgctcgcc agacatacac
       61 cggttccggt gcatttttaa cccgtttatc aatcgcgcct gcgcgccagc ctttccatgc
      121 ttcgcggaaa caaaatatcg ggataaggaa aaaaacggca acgacaaaaa actgctgtac
      181 atccataact actttccttg tttcttttgt aattcaagta actgttcggg tgtaacggcg
      241 ggatacccct gcgcatcttc cggcacctgg tgcatagccg gaatagggac cagcggccct
      301 aaaaaacgcg gttcacgttt aaaaatataa aggtcagcca gcgcaccgaa acgggcggca
      361 aattcgcgaa tacgtactga cagcatatct ttcggtgacg gtacggcata acactgagcc
      421 tgaatcccca tatgcagcgc aataaataat gctcgctcac agtggaaacg ttgggtgata
      481 ataatgaaat cattagtatc gaaaactttg cgtgtacgca cgatggaatc cagcgtacga
      541 aagcctgcgt aatcgagaac aatatctgat gggtcgacac cagcagcgat taaatctttg
      601 cgcatggtca tcggctcgtt ataactttgc aatgcgttat cgccgctcag taatagataa
      661 tttaccttac cgctgttata ggcattaatc gctccttgaa tgcggtagcg ataatactga
      721 ttaataacgc cggtacgata atattttgct gtgccgagca ccacgccgac ctggcggtag
      781 gggagatcct gcaattcgtc gtagatataa ggcgcggttt tccagctcat ccagcgatcg
      841 aggccgagca cagtcaacag cagcaagccg atcaggacta acaggctgag gaacacgcgc
      901 tttaacatgc aggagactcg agctaaggaa aatttccatc aggctacttt acccgctggt
      961 tttgcgcaag aaacggcaaa ctgtttgccg ggaatttcga caccaggaga gggctggcaa
     1021 gccggacaag gcgttcacgc cgcatccggc accaggctta agcgagaagc acgcggtcga
     1081 tactgtgaca gccgagagct ttcagcgttg cggaggtggc atcccactga atcaacggcg
     1141 catcggcttt ttctgccaga accgcgcgct ggatatccgg gtaatcataa cccttgagat
     1201 tcaacagaat taacgccccc tgcaacggcg gcagagtcgg gttgaatgcg gcgttttcag
     1261 cgtagctgcc gctgaaaata cgaccgtctt tacattccag cgcgacaccg cttggtgact
     1321 tactgtaagg catgtgcgaa cggtttgccg ctgcaatcgc cgcctgagaa agcgcatcac
     1381 cagtcagcgc atagccgtga tcctgctcgt ccatcagcag cgttttaatc tccagatctt
     1441 tcggcccaaa ggcatccggc agatagtcac gcagcgcgtg tgcctcgcgg cccggcaaat
     1501 gaatacgcag atccagaccg ctgttcagtt cattcataaa ctgacggcag tgaccacaag
     1561 gcgtatagtt aacggtgatg gctgcaagcg ctttttcacc actcaaccaa gcgtggctga
     1621 tcgcgctttg ttcggcatga acggtttgct gcattgttgc accaataaat tccatattgg
     1681 caccaaaata ccaggttccg ctcacaccgc gcgcaattgc gccaacatta aaattcgaca
     1741 atggcgtacg cgcacaggcc gccgccagcg gaagtagtgc gaatgccagc gcgtcttcgt
     1801 ccagtcccgt tgcgctcttc agcgatgaga cttgctcccc ggtcaacaaa gcggggaagt
     1861 acttgtctgc cagaataggt tccagtgcag attgcaagtt atccgcaagt tgggcaaaag
     1921 cggtttgaaa acgtggatgc atggcgttgc ctcataatca tgtaatggga taacagttta
     1981 cgaacccgtt atggctttat atgtgatctg aatctcatta attatgcaac acgtgaaact
     2041 tacatcaaaa atgcgcgacg tatcgcaaat tttagcccat tactgccaga ataatcggga
     2101 aaaggaacgg tgcgatcagc gaagtaatta tcccgcataa caccagcgct agcgaactaa
     2161 atgcaccttc ctgataatcc agctcggcgc agcgcgccgt accgagggcg tgcgaggcag
     2221 tccccattgc cagaccgcgc gcagctttgg tacgaatacg catcgcatta agcaatgtat
     2281 ggccaaatac cgcgccgagg atgccgacga aaatcacgca aactgcgctg attgccggaa
     2341 taccgccaat actgccgcca accgccattg caataggcgt ggtgactgat ttcggcagga
     2401 ttgacgcggc gatttccggt gaagcgccca tcaacaatgc cacggaagtc cccgtcacca
     2461 tcgcaaccac gctgccgata aaacagatgg tgatgatcga tttccagcgc gcgcggatct
     2521 ggtgtagctg ctcatataaa ggataggcca gcgcgacgac cgccggttgc agcagatcgt
     2581 ttagcacttc gctaccttta aagtaactgt cgtaagagat gccggtcagc attaaaaaag
     2641 gaatgatcac caccattgct accagcaacg ggttaagcag gggaaactta taccgtgccg
     2701 ccagtttgcg ggcggcaaaa aagacaatca acgtcagcgg taatgaccac cagatattcg
     2761 ccatcattct tctgatcctt tctgacctac gactttacgt tcaccgtgta ccagttgtga
     2821 actccagctc accaccagaa aaaccaccag cgtactgaca gcacaggaaa ccactaccgg
     2881 gccaaactgc gcgcggagca aatcaaaata ttgcatgacg cctacgccaa tcggcacaaa
     2941 caatagcgcc atatagcgaa tcagtacgta gcaccccgga ttgacccatt tcgccggaag
     3001 aatttgcaag gccagcagga caaacaggat cagcatcccg atgatgctgc ccggaatggt
     3061 taccggtagc agggaagcaa tgaaaatgcc tgcatacagg caggcataaa tcaggacgaa
     3121 agcgcgtaaa tattgccaga taatgttcag tgtcttactc ataatcaaca ccccagatga
     3181 gatggattca tcatacaatt aattcgtaaa acgtgctacc gatcacatta agaaatcagc
     3241 gcttattttt tcgctacccg gtattacgca atgagcagcg aactcaggca tacatcaagc
     3301 atagagaggg attgcagttt gcagcaatcc ctctttgcag acctttaatc cgctgaccat
     3361 aggcgctaac atagcgcctc atttttttgc gggtgatgat atgcgtgtgt tactggcacc
     3421 gatggaggga gtgcttgact ctctggtgcg tgaattgctg accgaagtta acgactacga
     3481 tctgtgcatc accgagtttg tccgcgtggt ggatcaactg ctgccggtaa aagtctttca
     3541 tcgcatttgc cctgagctac aaaacgccag ccggacacca tctggtacgc tggtgcgcgt
     3601 gcagttgtta ggtcagttcc cacaatggct ggcagagaac gccgcccgtg cggtggagtt
     3661 aggtttctgg ggcgtggatc tcaattgcgg ctgcccgtcg aaaacggtta acggtagcgg
     3721 cggcggggcg acgttactca aagatcctga actcatctac cagggggcaa aagcgatgcg
     3781 tgaagctgta ccggcgcatt tgcctgtcag cgtgaaagtg cgtctgggct gggacagcgg
     3841 tgagaagaaa tttgaaatcg ccgatgcggt tcaacaggct ggcgctacgg agctggtggt
     3901 gcatgggcgg acgaaagagc agggttaccg cgcggagcat attgactggc aggcgattgg
     3961 cgagattcgc cagcggctga atattccggt gattgccaac ggtgaaatct gggactggca
     4021 gagcgcgcaa caatgcatgg cgatcagcgg ctgcgacgca gtgatgattg gtcgcggggc
     4081 gctcaatatt cccaacctga gccgggtggt aaaatataac gaaccgcgaa tgccgtggcc
     4141 ggaggtggtt gctttgctgc aaaaatatac ccgtctggaa aagcagggcg ataccgggtt
     4201 atatcacgtt gcgcggatta aacagtggtt gagttatttg cgtaaagaat acgatgaagc
     4261 aacggaatta tttcagcatg ttcgggtgtt gaataactcc cctgatattg caagggctat
     4321 tcaggcaatt gatatcgaga aactctaaaa accatagtga tgtttttgac aatgtgttag
     4381 ctctttatca ataggccgca cggttgtttg gtggcgtcaa aatcgccgag gcgttccctg
     4441 aaggccgggg cagcccacat ggatgtgggc tgagggcgcg ttttacaggg atgttacctc
     4501 gcgcccgacc cggtagccgt aagggataag ccgagggcac cgcgcagcgg cgattttgtt
     4561 cgccagagcc cgggggggca gggggcggcg gcgattggtc gccccctgcg cgctccttgc
     4621 gccagtgatt atatgaagca atgcacatga caggagcgca acaggatgag agactgaaat
     4681 attctcgtta ttttcggatc tgatgacatt aattaatttt aaaatatcat cttaaatacg
     4741 ccagtcacca ccagtagccc aatcaggaaa ataatcaata cagcccagag tataattttc
     4801 attggcgttc ctttatttgt gagtgctgaa taagtatagg aaaaaacgaa gccctttgcc
     4861 gttatccata aacgattaag ataaatcgga agatatcatt tacttagtct atatttttct
     4921 gggtatgtcg ttttattgct actgatgtat tgccacgcga aactaaaaaa tatatcgcaa
     4981 cacacggtaa tctccgcgca ccgtttctta cctgattatt cccccatgaa tcgtgattcc
     5041 ttttatccag ccatcgcctg ttttccgctg ttactgatgc tggccgggtg tgcgcctatg
     5101 catgaaaccc gccaggcgtt aagccagcaa acgcccgctg cacaagttga caccgcatta
     5161 cccacggcgc tgaaaaatgg ttggccagac agccaatggt ggctggagta tcacgataat
     5221 cagctcactt cattaattaa caatgcacta cagaacgcac cggatatgca ggtggctgaa
     5281 cagcgcattt agctagctga agcccaggcg aaggcagttg ccactcagga tggtccgcag
     5341 atcgactttt cggcggatat ggagcggcaa aaaatgtcgg cagaagggct aatggggccg
     5401 tttgctctga acgatccggc cgcaggtacg accggtccgt ggtacaccaa cggaactttt
     5461 ggcttaacgg cgggctggca tctcgatatc tggggaaaga atcgggcgga ggttattgcc
     5521 cgcctgggta cggttaaagc acgggcggcg gaacgcgagc aaacccgcca attgctggct
     5581 ggcagcgtag cccgcctgta ctgggagtgg caaacccagg cggcgttaaa cacggtcttg
     5641 cagcaaatag aaaaagagca gaacaccatt atcgcgaccg atcgccagct atatcagaac
     5701 gggattactt cttcagttga cggtgtggaa accgatatta atgccagcaa aacccggcag
     5761 cagctcaacg atgtcgcggg gaaaatgaaa attattgagg cacggttaaa tgcactcaca
     5821 aatcaccaga ctaagtcatt gaagcttaaa ccggtcgcgt tgccgaaagt ggcaagccag
     5881 cttcctgatg aactggggta ctccttactg gcccggcggg cagatttgca ggcggcgcac
     5941 tggtacgttg agtcatcgtt aagcactatt gatgcggcaa aagcggcatt ttatcctgac
     6001 atcaacctga tggccttcct gcaacaggat gcgttgcact taagcgatct gttccgtcat
     6061 tccgcgcagc aaatgggcgt tacggcaggc ctgacgctac ctattttcga tagtggtcgt
     6121 cttaacgcca atctcgatat cgcaaaagcc gaaagcaact tgtctatcgc cagctacaac
     6181 aaagcggtgg ttgaagcagt gaatgacgtg gcgcgggcag ccagtcaggt tcagacactg
     6241 gcggagaaaa accagcatca ggcgcaaatt gagcgcgatg ccttgcgtgt ggtaggtctt
     6301 gcgcaggccc gctttaacgc gggcatcatt gctggttccc gcgtcagcga agccagaatc
     6361 ccggcgctgc gtgagcgggc caatggcctg ttattgcaag ggcagtggct ggatgcctcc
     6421 attcgactca ccggtgcgct gggcgggggt tacaaacgct gatgatatgc gccttctata
     6481 cttaacgttt attcagcgtt aagtggagaa ctcgatggca taggtcgcga taattaccgc
     6541 ttccgattcg gggatcggca aagagtgcgc gttattactg gcgcagcagg ggtttgatat
     6601 tggcattacc tggcactcag atgaagaagg ggcaaaagat accgcgcgtg aggtggttac
     6661 ccacggcgta cgtgcggaga tcgtgcaact ggatctcggc aatctgccag aaggtgcgca
     6721 ggcgctggag acactcatcc agcgatttgg gcgtattgat gtgctggtga ataatgcggg
     6781 tgcaatgacc aaagcgccgt ttcttgatat ggcttttgat gagtggcgca agatttttac
     6841 cgttgatgtt gatggtgcat ttttatgctc gcaaattgcg gctcgtcaga tggtgaaata
     6901 agggcagggc ggtcgcatca tcaacattac gtcggtacat gaacatacgc cgctgccgga
     6961 tgccagcgcc tacacagccg ctaaacatgc gctcggcggg ttaaccaaaa cgatggcgct
     7021 ggagttggtc aggcataaga ttttggtgaa cgcagtcgcg cctggggcga tagccacgcc
     7081 aatgaacagc atggatgata gcgacgtgaa gcccgacgcg gagccttcga ttcctctacg
     7141 gcgttttggc gcaacgcatg agattgccag cctggtggtg tggctttgtt cggagggcgc
     7201 aaattacacg acagggcagt cgttgatagt ggatggcggc tttatgttgg cgaatccgca
     7261 gttcaaccca gaatagcgca gtgaggtgcg tggatatttc aatgggttag gccagataag
     7321 acaccagcgt cgcatcaggc atggaacacc cggtgccgga tgtggagtga atgcttatct
     7381 gtggcaactg ctttacgcct gattatccgg ctttttcttc ccgcgccgtt tcagccacca
     7441 gcgcacgccc accaccagaa ccacgaccag aatcaaccag acccagtgct tcaaatgctg
     7501 gtcgagattg tgcaaccacg gcgcaatcac ctgaccacca gcgtaaccaa tagtggtaaa
     7561 aatcaacgcc caggcaaatg cgccgagaat attcagcggc agaaagattt tcggcggcag
     7621 ctggctggca ccaatcagcg tcgggccaat cacccgaaag ccatacataa agcgcgtacc
     7681 aatgacaaac agatacggat ggcgttggat aagtttctgt gcccgctcaa ttttatcctg
     7741 atgtttcgag aaacggcgta acagcttgcc gccaaaccgc cgcccgcaca gatagagcac
     7801 ctggtcgcca atcatgccac caagcgccac agaaagtacc accagtggga actttaatag
     7861 cccctgatgt gccgcaacgc ctcccagcaa agtcacggtt tcaccttccg ccaggctacc
     7921 gataaccagc gcggcataac catattgtga gatgagtgta ttgagatcca taggccagtt
     7981 tgtctccctt tggttgcctg taagcataca ccctgtgaat cattcacgct gaaaaaggac
     8041 atacatcaga cggtgcaaat tacatggatg aacaaaaaat aatcacaagc tggcttatac
     8101 ttgaactgtc aggttcatga actcacaaca agggggcggt atgagccatg tctggggact
     8161 gttttcccat cccgatcgtg aaatgcaggt gattaatcgc gaaaacgaaa cgatttctca
     8221 ccattacacc caccacgttt tgctgatggc ggcgattccg gtgatttgcg ccttcattgg
     8281 cactacacag attggctgga attttggcga tggcactatc ctgaagttat cctggtttac
     8341 cggactggcg ctggctgtct tgttttacgg cgttatgctg gctggggttg cggtgatggg
     8401 gcgggtcatc tggtggatgg cgcgcaatta cccgcagcgt ccgtctcttg cgcactgcat
     8461 ggtcttcgcg ggctacgttg caactccgct gtttttaagc ggtctggtgg cgctttatcc
     8521 actggtctgg ttgtgtgcgc tggtcggcac ggtggcgctg ttttacaccg gctacctgct
     8581 ttaccttggt attccgagct tcctgaatat caacaaagag gaaggcctta gcttctccag
     8641 ttcgacgctg gcgattggcg tgctggtact ggaagtgctg ctcaccctaa ccgttatact
     8701 atggggctac ggttatcgtt tgttctagaa agttctttga cgttgcattg ctggcgtaaa
     8761 tgtcagcaat gcagcattta ctcacgattc tcctttgacg atcgcacttt ttgctcgtta
     8821 tgatggcgtc gctagcctca gtaaatcctt actccggcgg tgcgcaaccc gtgcgcgtga
     8881 accactatct gaatgctcat catgccgaaa tttcgagttt ctttatttag cttggccctg
     8941 atgctggctg tgccttttgc accgcaggcc gttgcgaaaa cggcagccgc taccaccgct
     9001 tcacaaccgg aaattgcctc cggtagcgcg atgattgttg atctgaatac caacaaagtg
     9061 atctattcga accacccgga tctggtgcgt ccgattgcgt ctatcagcaa attaatgacc
     9121 gcgatggttg tgctggatgc acgactgccg ctggatgaaa aactaaaagt ggatatcagc
     9181 cagacgccgg agatgaaagg ggtctattcg cgcgtacgac tgaatagcga aatcagccgt
     9241 aaagatatgc tgttgctggc gctgatgtct tcagaaaacc gcgcggcggc aagccttgcg
     9301 caccattatc ccggtggtta caaagccttt attaaggcaa tgaatgcgaa agcgaaatcg
     9361 ctcggaatga acaacacgcg ctttgttgaa cctaccggat tgtcggtgca taacgtttca
     9421 actgcccgtg acttaaccaa actgctcatt gccagcaaac aatatccgtt gatcgggcag
     9481 ttaagtacta cccgggaaga tatggcaacc ttctctaacc cgacgtatac gctgccgttc
     9541 cgcaatacta atcatctggt gtatcgcgat aactggaata ttcagttaac caaaaccggc
     9601 tttaccaatg cggcgggcca ttgtctggtg atgcgtacgg ttatcaataa taaaccggtg
     9661 gcgctggtag tgatggacgc gtttggcaaa tatacccatt ttgccgatgc cagccgcctg
     9721 cgtacctgga ttgaaaccgg taaagtgatg cctgtgccgg cagcagcgtt gagctataaa
     9781 aaacaaaaag ccgcccaaat ggcggcggcg gggcagacgg cacagaacga ttaagtgaat
     9841 tcggatggcg atactctgcc atccgtaatt tttactccac ttcctgccag tttttccgtt
     9901 tactggtttt accgatcccc ggattcatgc tattggtcgg atcattctcg cgatagaact
     9961 tctgcaacgt ctccggtgct ttatacaaat gaccgacgtt atgctcggca gggtactgcg
    10021 cgccgcgctg ctgtagcagt tccagcatct gttcttttaa cgcatgcaca tccacgcctt
    10081 ttttcactat gtaatcctga tggaagacat agcacataaa atggccgtaa tagagcttgt
    10141 gcaccagctg gctgtcgatc tccggcggta aatgctcata ccactcggtg tcgttacgcc
    10201 gcagagcgat atccagtgcc agaatgtctt cgacttcatc ggaatgcacc gcctgataac
    10261 gaattgctgc gcccgcagcg gcgaaacggt gtaaaaacgc tttgctacct tcctccggcg
    10321 tacagacaaa gaaatcgccc tcggcctgtt tgaaataatc caccagccac gatttggctt
    10381 caccgacgcc atcgcctgcc atttttaaca gcagatgatg ctcgtattta tcgcgccagt
    10441 ttttcatgcg cggcggtaaa tggctgggga acaggtgacc gaatttttgc attgcacggt
    10501 cagtaaaatg cggacggaag aatttcactt tctccagcat cgcatcggtg cgtcccttga
    10561 gattaaagaa gaacggcatc ttgtcggtgc ctaacttatc aatcatcagg aaggtgtctt
    10621 tgccgtattt ttccgcaata tcgtagatat cccggtgcat atattccccg gcaaccggca
    10681 gattttcgaa gttagccaga atatgacggc ggatttcggt cagcacttcc ggctggttgg
    10741 tgccgatata aaacacctgc tgattttttt ccgcttcgaa ggtatcaaga cgtaccgcaa
    10801 agacggccag cttcccggcg caaccagaag attcaaataa ccgatccgga tcggcgttat
    10861 aacgtgcggg cgtgtcggct tcaatatcac gaacgcggtg gacatagtca taatcgtggg
    10921 cgtgacgacc atcgtgacgc acatcgtcat ctttgatgcg atcgtcatcc agcttgctaa
    10981 ggatctgctc cggcgtttcg cccagatcaa tccccagatg gttcaccagc gtcagtttgc
    11041 catcttcatc tatacgcgcg aataacgaca tttcggtata cgccgggcca cgttgcacca
    11101 gcgagccgcc ggagttgtta caaataccgc cgatgaccga tgcgcctata cacgatgatc
    11161 caatcaccga atgcggttcg cgtcccagcg gtttgagggc tttttccagc gaatagagcg
    11221 tggtgcccgg ataggccagc acctgttcgc ccttgccgag aacgtgcagc ttgtcgagac
    11281 gcagggtgct gatgatcacg atatcgcgat catagtcgtt accgtttggc gtcgatcctt
    11341 cggtcaggcc tgtattggca gcctgcatca gaataatttt gtcggcggtg acgcaggctt
    11401 tcagcacccg ccacaattcc agtagtgagc cagggaaaac gacagccagc gcgtcgccct
    11461 gaccagaacg gaagcccttg cgatagcggg ccgtttttgc gggatcggtg agcaggtgtg
    11521 aatgaccgac cagacgggca agttcattca aaaaggcttt attatcagtt gttgtcatgg
    11581 aagacatttt ccactccttg tggtggcgaa aaaatatcac aaactagaat agcgccttgt
    11641 atgacaattc agcgagtgaa tattttgaaa attcagacaa taaactgagc attctttttc
    11701 tctctccatc atgcttatgg caaactacgg tttttgcacc attttcgctg ccacgtcggg
    11761 caacaaagga agaaaaatcc atatgaaatg gctatgttca gtaggaatcg cggtgagtct
    11821 ggtcctgcag ccagcactgg cggatgattt attcggtaac catccattaa cgcctgaagc
    11881 gcgggatgcg ttcgtcaccg atctgctcaa gaaaatgacg gttgatgaga aaattggtca
    11941 gctgcgctta atcagcgtag gcccggataa tccgaaagag gcgatccgcg agatgatcaa
    12001 agacggtcag gttggggcga ttttcaacac cgtaacccgt caggatatcc gcgccatgca
    12061 ggatcaggtg atggaattaa gccgcctgaa aattcctctt ttctttgctt acgacgtgct
    12121 gcacggtcag cgcacggtgt tcccgattag cctcggtctg gcctcgtctt ttaacctcga
    12181 tgcggtgaaa acggtcgggc gtgtctctgc ttatgaagcg gcagatgatg gcctgaatat
    12241 gacctgggca ccgatggtcg atgtctcgcg cgatccgcgc tggggacgtg cttccgaagg
    12301 ttttggcgaa gatacgtatc tcacctcaat aatgggtaaa accatggtgg aagcgatgca
    12361 gggtaaaagc ccggcagatc gttactcggt gatgaccagc gtcaaacact ttgccgcata
    12421 cggcgcggta gaaggcggta aagagtacaa caccgtcgat atgagtccgc agcgcctgtt
    12481 taatgattat atgccgccgt acaaagcggg gctggacgca ggcagcggcg cggtgatggt
    12541 ggcgctgaac tcgctgaacg gcacgccagc cacctccgat tcctggctgc tgaaagatgt
    12601 tctgcgcgac cagtggggct ttaaaggcat caccgtttcc gatcacggtg caatcaaaga
    12661 gctgattaaa catggcacgg cggcagaccc ggaagatgcg gtgcgcgtgg cgctgaaatc
    12721 cggaatcaac atgagtatga gcgacgagta ctactcgaag tatctgcctg ggttgattaa
    12781 atccggcaaa gtgaccatgg cagagctgga cgatgctgcc cgccatgtac tgaacgttaa
    12841 atatgatatg gggttgttta acgacccata cagccatttg gggccgaaag agtctgaccc
    12901 ggtggatacc aatgccgaaa gccgcctgca ccgtaaagaa gcgcgtgaag tggcgcgcga
    12961 aagcctggtg ttgctgaaaa accgtctcga aacgttaccg ctgaaaaaat cggccaccat
    13021 tgcggtggtt gggccactgg cggacagtaa acgtgacgtg atgggcagct ggtccgcagc
    13081 cggtgttgcc gatcaatccg tgaccgtact gaccgggatt aaaaattccg tcggtgaaaa
    13141 cggtaaagtg ctgtatgcca aaggggcgaa cgttaccagt gacaaaggca ttatcgattt
    13201 cctgaatcag tatgaagaag cggtcaaagt cgatccgcgt tcgccgcaag agatgattga
    13261 tgaagcggtg cagacggcga aacaatctga tgtggtggtg gctgtagtcg gtgaagcaca
    13321 ggggatggcg cacgaagcct ccagccggac cgatatcact attccgcaag gccaacgtga
    13381 cttgattgcg gcgctgaaag ccaccggtaa accgctggtg ctggtgctga tgaacgggcg
    13441 tccgctggcg ctggtgaaag aagatcagca ggctgatgcg attctggaaa cctggtttgc
    13501 ggggactgaa ggcggtaatg caattgccga tgtattgttt ggcgattaca acccgtccgg
    13561 caagctgcca atttccttcc cgcgttctgt cgggcagatc ccggtgtact acagccatct
    13621 gaacaccggt cgtccgtata atgccgacaa gccgaacaaa tatacttcgc gttattttga
    13681 tgaagctaac ggagcgcttt atccgttcgg ctatggtctg agctatacca ctttcaccgt
    13741 ctctgatgtg aaactttctg cgccgaccat gaagcgtgac ggcaaagtga cggccagcgt
    13801 gcaggtgacg aacaccggta agcgcgaggg ggccacggta gtgcagatgt acttgcagga
    13861 tgtgacggct tccatgagtc gccctgtgaa acagctgaaa ggctttgaga aaatcaccct
    13921 gaaaccgggc gaaactcaga ctgtcagctt cccgatcgat attgaggcgc tgaagttctg
    13981 gaatcaacag atgaaatatg acgccgagcc tggcaagttc aatgtattta tcggcactga
    14041 ttccgcacgc gttaagaaag gcgagtttga gttgctgtaa tgctttagta agggcgcgac
    14101 gtttgcgccc tttgcctgat gttcatatct taacttgctg aattttcgcc ctttgtaggc
    14161 cggataaggc gctcgcgccg catccggcag gaaacgccgg accgaatatg cctcttaagt
    14221 atttatctga ttctgcaact gaatccttcc gctcaggcta actctgtcat tatcaactat
    14281 gcttttctct taattcgctg aaaacaacag taaaagaatg aggaaagcat catgccactc
    14341 ttaaagctct gggcaggttc actggttatg ttggcagccg tgagcctgcc gctacaggcg
    14401 gcttcccccg ttaaagtcgg ttcaaaaatc gataccgaag gcgcgctgct cggcaacatc
    14461 atcttgcagg tactcgaaag ccacggcgta ccgacggtga ataaagtgca actcggaacg
    14521 actcctgtgg ggcgcggggc gattacttcc gatgaactgg atatctatcc ggaatatacc
    14581 ggcaatggcg ctttcttctt taaagatgaa aacgatgcag cgtggaaaaa tgccgggcag
    14641 ggctacgaga aagtcaaaaa gctcgatgcg gagcacaaca agttaatctg gctgacgccc
    14701 gcgcctgcaa ataacacctg gactatcgcc gtgcgtcagg atgtggcaga gaaaaacaaa
    14761 ctcacttcgc ttgccgacct gagtcgttat ctgcaagagg gcggcacctt caaactggca
    14821 gcctcggcag agtttatcga acgcgccgat gcgttaccca cgtttgaaaa agcctatggc
    14881 tttaagctcg gtcaggatca gttgctgtca ctggctggtg gcgacacggc ggtgacgatt
    14941 aaagccgctg cccagcaaac ctctggcgtt aatgccgcaa tggcttacgg cactgacggt
    15001 ccggtcgcgg cgctggggct gcaaacctta agcgatccgc aaggcgtgca acctatctac
    15061 gcgcctgcac cagtggtgcg tgaatcggtg ttgaaggagt atccgcaaat ggcacagtgg
    15121 ctacagccag tcttcgccag cctcgatgca aaaacattgc agcaactgaa tgccagcatt
    15181 gcagtggaag gactggatgc caaaaaagtg gctgccgact acctgaaaca aaaagggtgg
    15241 gcgaagtaat ttcccgtgac ttatctacgt attaatcctg ttctggcgct gctgttgttg
    15301 ctgacggcaa tcgcagcggc gctgccgttt atcagttacg cgcctaatcg tttagtctcg
    15361 ggtgaagggc ttcatctctg gcaactgtgg ccgcaaacga tctggatgct ggtgggcgtt
    15421 ggttgcgcct ggctgacagc ctgttttgtc cccgctaaaa aaggcagcat ttttgcactc
    15481 attctggcgc aattcgtctt cgtattgttg gtgtggggag ctggaaaggc ggcgacccaa
    15541 ctggcgcaaa atggcagtgc gctggcgcgt accagcctcg gcagaggttt ctggctggct
    15601 gctgcgctgg cattgctggc ctgtagcgat gccatccgcc gaatctccac gcatccgctg
    15661 tggcgctggt tgttgcatat gcagattgcc attattccgc tgtggttgct gtactccggc
    15721 acgcttaacg atctctcact aatgaaagaa tacgccaacc gtcaggatgt gtttgacgac
    15781 gcgctggcac aacatctgac gttgctgttt ggtgcggtgc tgcctgcgtt agtgattggt
    15841 gtgccgttgg gcatctggtg ctacttttcc actgcgcggc agggggcaat tttttctctg
    15901 ctcaatgtca ttcagaccgt gccttcggtg gcgctctttg gcctgttgat tgcgccgctt
    15961 gccgcgctgg ttacggcctt tccgtggctg gggaagctcg gcatagcagg aaccggaatg
    16021 acacccgcac tgattgcgct ggtgctctat gccttgctgc cgctggtgcg aggcgtggta
    16081 gtcggtttga accagatccc gcgcgatgtg ctggagagcg ccagagcgat ggggatgagc
    16141 ggggcgcagc gtttcctgca tgttcagtta ccgctggcgt taccggtatt tctgcgcagc
    16201 ctgcgggtgg tgatggtgca aactgtaggt atggcggtga ttgcggcgtt aatcggcgca
    16261 ggcggttttg gtgcgctggt tttccagggg ctgctaagca gcgccattga tttagtgttg
    16321 ctgggggtga tcccggtaat tgttctggcg gtgcttaccg acgcgctgtt cgatttgctt
    16381 atcgcactgc tgaaggtgaa acgtaatgat tgaatttagc catgtcagca aactgttcgg
    16441 cgcacaaaaa gccgttaacg atctcaatct caattttcag gaagggagtt tttcggtgct
    16501 gattggcaca tctggctccg gcaaatccac caccctgaaa atgattaacc gcctggtgga
    16561 gcatgacagc ggagtgatcc gctttgccgg agaagaaatt cgctcgctgc cagtactgga
    16621 gttgcgccgc cggatgggct atgccattca atctattggc ctgttccccc actggagcgt
    16681 ggcgcaaaac atcgctaccg tgccgcaatt acaaaaatgg tcgcgggcgc ggattgacga
    16741 tcgtatcgac gaattaatgg cgctactggg gctggagtca aatttgcgtg agcgttatcc
    16801 gcatcagctt tccggtggtc agcagcaacg tgtgggagtg gcgcgtgcac tggctgccga
    16861 tccgcaagtc ttactgatgg atgaaccttt tggcgcactg gacccggtaa cgcgcggcgc
    16921 gttgcaacaa gagatgacgc gcattcaccg tttgctgggg cgcactattg tgctggtcac
    16981 tcatgatatt gatgaggcgc tacggctggc agaacatctg gtattgatgg atcacggtga
    17041 agtggtgcag caggggaatc cgctgacgat gctgactcgt ccggcgaatg attttgtccg
    17101 ccagtttttt ggacgtagtg aactgggggt gcacctgctt tcgttacgta gcgtggcgga
    17161 ttacgtgcgt cgcgaagaac gggcagaagg tgaggcactg gcagaagaga tgacgctacg
    17221 cgatgcgctc tccctgtttg tcgcgcgggg atgcgaggtg ctgccggtgg tgaacacgca
    17281 gggccagcct tgcggcacgc tgcattttca ggatctgctg gtggaggcgt aagcgtatga
    17341 agatgttgcg cgatccgctg ttctggctca ttgctctttt tgtggcgctg attttctggc
    17401 tgccttacag ccagccgctg tttgctgcct tgttcccaca actgccacga cccgtttatc
    17461 agcaagaaag ttttgcagct ctggcactgg ctcatttctg gctggtggga atttcgagtt
    17521 tgtttgcggt gatcattggc acaggtgccg gaattgctgt tactcgcccg tggggcgcgg
    17581 aatttcgccc actggtggaa actattgccg ccgttggaca gacttttccg cctgtcgcag
    17641 tgctggcgat tgccgttccg gtgatcggct ttggtctgca accagcgatt atcgccttga
    17701 tcctttacgg tgtgctgccc atcctgcagg tgacacttgc cgggctggga gcgattgatc
    17761 ccagcgtgac agaagttgcg aaaggtatgg gaatgagtcg aggtcagcga ctgcgtaagg
    17821 tcgagctacc gctggcggct ccggtgattc tggcgggcgt gcgaacttcg gtgattatca
    17881 acattggtac ggcgacgatc gcctcaacgg taggggccag tacgctgggt acgcccatca
    17941 tcatcgggct tagcggattt aataccgcgt atgtgatcca gggggcgtta ctggtggcac
    18001 tggcggcgat catcgcagac cgcctgtttg aaaggctggt gcaggcgctt agccagcacg
    18061 caaaataaag gtataacctg cgagcatgac gccaccaatt ccgcctaacg ccataaacag
    18121 gaacagggcg atgaccccaa ttttagctat gcgcataatg cactccttat gttaacgaaa
    18181 ggattgtaca gtaaagcgca tttgttaacg aatcattaaa taccgagtgg gaaaatatca
    18241 tggccttgtt cttgccaact ggtgagttgc tgctgttggg cggaggttcg attttcaccg
    18301 caccacacca gcaatgtacg gccttcgaat agttcagggc gtagttgatt gagcgagtgg
    18361 gcgagcgagg acatcaatgc gccatccttg ttgactggca atccagccct ccagccacag
    18421 acgggtggta tcctgaatat tccagccaac caccagcgca tctttaccct gttttttacg
    18481 tgccgaagcc agacaaatgg cgatgtagtt gatcagtacg ccgtcgagga tcgccagcag
    18541 cgcctggaga gtcggttgtt ggcactgaag ccgtcggcgc agaggaataa acagatgtgt
    18601 ggtgagtgtc tgggcggggt aatcctgacc gcgctctttg atccacgttc gcaggctatg
    18661 tagattgc
//
LOCUS       DAZPAB010000067        18666 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZPAB010000067 DAZPAB010000000
VERSION     DAZPAB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.067"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            179..628
                     /gene="alaE"
                     /locus_tag="ACHUPG_002044"
     CDS             179..628
                     /gene="alaE"
                     /locus_tag="ACHUPG_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR0861665.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(665..1009)
                     /locus_tag="ACHUPG_002045"
     CDS             complement(665..1009)
                     /locus_tag="ACHUPG_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR0861666.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1161..1490
                     /locus_tag="ACHUPG_002046"
                     /pseudo
     CDS             1161..1490
                     /locus_tag="ACHUPG_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
     gene            1738..1983
                     /gene="nrdH"
                     /locus_tag="ACHUPG_002047"
     CDS             1738..1983
                     /gene="nrdH"
                     /locus_tag="ACHUPG_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR0861667.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            1980..2390
                     /gene="nrdI"
                     /locus_tag="ACHUPG_002048"
     CDS             1980..2390
                     /gene="nrdI"
                     /locus_tag="ACHUPG_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFR0861668.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            2363..4507
                     /gene="nrdE"
                     /locus_tag="ACHUPG_002049"
     CDS             2363..4507
                     /gene="nrdE"
                     /locus_tag="ACHUPG_002049"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR0861669.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4517..5476
                     /gene="nrdF"
                     /locus_tag="ACHUPG_002050"
     CDS             4517..5476
                     /gene="nrdF"
                     /locus_tag="ACHUPG_002050"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR0861670.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            5831..7033
                     /gene="proV"
                     /locus_tag="ACHUPG_002051"
     CDS             5831..7033
                     /gene="proV"
                     /locus_tag="ACHUPG_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR0861671.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            7026..8090
                     /gene="proW"
                     /locus_tag="ACHUPG_002052"
     CDS             7026..8090
                     /gene="proW"
                     /locus_tag="ACHUPG_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR0861672.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8148..9140
                     /gene="proX"
                     /locus_tag="ACHUPG_002053"
     CDS             8148..9140
                     /gene="proX"
                     /locus_tag="ACHUPG_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR0861673.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9332..10511
                     /locus_tag="ACHUPG_002054"
                     /pseudo
     CDS             9332..10511
                     /locus_tag="ACHUPG_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            10635..11372
                     /gene="ygaZ"
                     /locus_tag="ACHUPG_002055"
     CDS             10635..11372
                     /gene="ygaZ"
                     /locus_tag="ACHUPG_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR0861674.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11362..11697
                     /gene="ygaH"
                     /locus_tag="ACHUPG_002056"
     CDS             11362..11697
                     /gene="ygaH"
                     /locus_tag="ACHUPG_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR0861675.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            11788..12318
                     /gene="emrR"
                     /locus_tag="ACHUPG_002057"
     CDS             11788..12318
                     /gene="emrR"
                     /locus_tag="ACHUPG_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR0861676.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12445..13617
                     /gene="emrA"
                     /locus_tag="ACHUPG_002058"
                     /pseudo
     CDS             12445..13617
                     /gene="emrA"
                     /locus_tag="ACHUPG_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            13634..15172
                     /gene="emrB"
                     /locus_tag="ACHUPG_002059"
     CDS             13634..15172
                     /gene="emrB"
                     /locus_tag="ACHUPG_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR0861677.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15236..15751)
                     /gene="luxS"
                     /locus_tag="ACHUPG_002060"
     CDS             complement(15236..15751)
                     /gene="luxS"
                     /locus_tag="ACHUPG_002060"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR0861678.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(15901..17457)
                     /gene="gshA"
                     /locus_tag="ACHUPG_002061"
     CDS             complement(15901..17457)
                     /gene="gshA"
                     /locus_tag="ACHUPG_002061"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR0861679.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(17530..17958)
                     /locus_tag="ACHUPG_002062"
     CDS             complement(17530..17958)
                     /locus_tag="ACHUPG_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR0861680.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(17955..18521)
                     /gene="yqaB"
                     /locus_tag="ACHUPG_002063"
     CDS             complement(17955..18521)
                     /gene="yqaB"
                     /locus_tag="ACHUPG_002063"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR0861681.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4441 a         4826 c         4723 g         4676 t
ORIGIN      
        1 tacaaaaaac gagccgttac ggaatatttt atctacaaaa actaactaaa taaaaatttt
       61 tcactaattg attagtcata gacagcgata tacgctatgc aaaaatgcag atggcaatga
      121 gatccactgc tttcatctcc attaacatcc cattacgctt ttattaagga gcattagcat
      181 gttctcaccg cagtcacgct tgcgtcatgc agttgcagat acgttcgcga tggttgttta
      241 ctgttctgtc gtgaacatgt gtattgaagt tttcctctcc ggaatgagct tcgaacagtc
      301 tttttattcc agattggtag cgattccggt gaacatctta attgcatggc catacggtat
      361 gtaccgcgat ctgtttatgc gcgcggcacg caaagttagc ccgtcgggct ggataaaaaa
      421 tctggctgat atcctggctt atgtgacgtt ccagtcaccg gtgtatgtgg cgatcttgtt
      481 agtggtgggc gcagactggc atcagattat ggcggcggtc agttcaaaca tcgttgtttc
      541 gatgttgatg ggggcggttt atggctactt cctcgattat tgccgccgac tgtttaaagt
      601 cagccgttac cagcaggtaa aagcctgact cttcttttcg cgactggcat cgccagtcgc
      661 gtctttaact ggcttcgcca aataaaccat tcaaataacg ttcaagcgca acacgcgaac
      721 taaagccatg cgggataccg taatgctcgt gtcgctcgcc tgctaaatag agcggaaatt
      781 gctggtaatg atcgcaagtt ttaatatcgg aagcgggttc agccagtgtt gaactgcgtt
      841 cttttaatgt tggatgaatt accagagcgg tacgtcccat tcgcgcttcg cgattaacat
      901 agacataatt ttccccacgg cggtaaccat acgctttctg ggttaccaca tcgacagtaa
      961 aaccgacttt ttcaagtacg cgcgccacct cgtctggtcg taaatacata tttgatcctc
     1021 gttattttca accgcgggcc taccttacct gattgcgcat tcacaacgct ttcagaaaag
     1081 tccataaagc cgcgatgaac ctcagttaag aaaattatgg tctacactga aaattacatc
     1141 gaattctaat gggggatcat atgtttaacc gaccgaaccg caacgacgtt gatgatggcg
     1201 tgcaggatat ccagaatgat gtcaatcaat tagctgacag cctcgaatct gtattgaagt
     1261 cctggggcag cgacgccaaa ggggaagctg aagccgcacg cagtaaagct caggcgttgc
     1321 tgaaagaaac cagggcgcga atgcatggtc gtactcgcgt ccagcaagcc gcgcgcgatg
     1381 ccgttggctg agcggattct tttgttcgtg aaagaccctg gtgtagcgtg ggtacagcag
     1441 ctgcggtagg tatttttatc ggcgcactgt taagcatgcg caaatcgtag tgcaaaaatg
     1501 ataataaata cgcgtctttg accccgaagc ctgtcttcgg ggtttctttt tgcctggtga
     1561 atcacaaaaa tccccctccc ccgtcacgct catatccagg gtaatttcga tcactatttg
     1621 ctatatattg tgtagttgaa tcttttttca actacatcta gtatctctgt atcaacagag
     1681 agacaacccg acgcgtatca tcgcgccgta tcttcatttt aaacggaaat acgaatcatg
     1741 cgcataacta tttatactcg taacgattgc gttcagtgcc acgccaccaa acgggcgatg
     1801 gaaaaccggg gctttgattt tgaaatgatt aatgtcgatc gcgttcctga agcagcggaa
     1861 gcgttgcgtg ctcagggctt tcgtcagttg ccggtagtga ttgctggcga tcttagctgg
     1921 tctggtttcc gtccggacat gattaaccgt ctgcatccag cgccacacgc ggccagtgca
     1981 tgagccagct cgtctacttc tccagcagct ccgaaaacac gcagcgtttt atcgaacgtt
     2041 taggtctgcc cgcggtgcgc atcccgctca atgagcggga acggattcag gtagacgagc
     2101 cttacatcct gatcgtgccc tcttacggcg gcggcggtac ggctggcgcg gtgccacgac
     2161 aggtaattcg ctttttaaac gacgagcaca accgggcgtt gcttcgcggc gttattgctt
     2221 ctggtaatcg caactttggt gaggcgtatg gccgcgccgg agatgtgatt gcccggaaat
     2281 gcggcgtgcc gtggctgtac cgttttgaac tcatgggtac gcaaagcgat atcgaaaacg
     2341 ttcgtaaagg agtaaccgaa ttttggcaac gacaaccgca gaatgcctga cgcaggaaac
     2401 gatggattac cacgcgctga atgcgatgct taatctttac gatagcgcag gtcgcattca
     2461 gttcgataaa gaccgccagg ccgttgacgc ctttattgcg acgcatgtgc gtccgaacag
     2521 tgtgaccttc agtagccagc agcagcgcct gaactggctg gtcaacgaag gttactatga
     2581 tgaaagcgtt cttaatcgct actctcgcga ctttgtcatt acgctgtttg cccacgcaca
     2641 caccagcggt tttcgtttcc agacattcct cggggcatgg aagttttaca ccagctatac
     2701 gttgaagaca ttcgacggta aacgttatct ggaagatttt gccgatcgag tcacgatggt
     2761 ggcgctgacg ctggcacaag gcgatgagac gctggcgttg caactgaccg atgaaatgct
     2821 gtcaggacgc tttcagccag ccacgccaac attcctcaac tgcggtaagc agcagcgcgg
     2881 cgaactggtt tcctgttttt tgctgcgtat tgaagacaat atggagtcga ttggtcgggc
     2941 ggtaaattcc gcactgcagc tgtcgaaacg cggcggcggc gtagcatttt tgctgtcgaa
     3001 tctgcgggaa gcgggcgcgc caattaaacg tattgaaaat caatcttctg gcgtaattcc
     3061 ggtgatgaaa atgttggaag acgcattttc ctatgccaac caactcggcg cgcgtcaggg
     3121 ggcaggtgca gtctatttac atgctcatca tcccgatatt ctgcgttttc tcgacacgaa
     3181 acgggaaaat gccgacgaaa aaatccgcat taaaacactg tcgctgggcg tggtgatccc
     3241 ggatatcact ttccatctgg caaaagagaa tgcgcagatg gcgctgtttt cgccctatga
     3301 cgttgagcga gtttatggca agccgtttgc cgatatcgcc attagcgaac actatgacga
     3361 actggttgcc gatgaacgca tccgcaaaaa atacctcaac gcccgtgatt tcttccagcg
     3421 actggcagaa atccagtttg agtccggcta tccctacatc atgtatgaag acacagtaaa
     3481 ccgtgctaac cctatcgccg ggcgcataaa tatgagtaat ctctgctcag aaattttgca
     3541 ggttaacagc gcctcagagt atgacgagaa tctcgactat gcccgcacag gccatgatat
     3601 ttcctgcaat ttaggttcgt tgaatattgc gcacaccatg gattcccccg atttttcccg
     3661 cacggtagag actgccgtgc gcggtttaac ggcggtgtca gatatgagtc atatccgcag
     3721 cgtgccgtcc atcgaagccg gaaatgccgc ctcgcacgcc atcggactgg ggcagatgaa
     3781 tttacatggt tatctggcga gggaaggcat cgcttatggt tcgccggaag ccctggattt
     3841 caccaatctc tatttctata ccatcacctg gcacgcactg cgtacctcga tgttgctggc
     3901 gcgcgaacgc ggtgaaacct tcgccgggtt caaacagtca cgctatgcca gtggtgaata
     3961 tttcagccaa tatctgcaag gtaactggca gccgaaaacg gcgaaagttg gcgaactgtt
     4021 tgcccgcagc tgtattacgt tacctacccg tgagatgtgg gcgcaactgc gcgacgacgt
     4081 gatgcgctac ggcatataca accagaatct ccaggcggtg ccgccaaccg ggtctatctc
     4141 ttatatcaac catgctacgt cgagtattca tccgattgtg gcgaaagtag agatacgcaa
     4201 agagggcaaa acaggacgcg tttactaccc tgccccgttt atgactaacg aaaatctggc
     4261 gctgtatcag gacgcttacg aaattggcgc agaaaagatc atcgacacct acgcggaagc
     4321 gactcgccat gtcgatcagg ggctgtcgct gacgctgttt ttccccgata ccgccaccac
     4381 tcgcgatatc aacaaagcgc agatttacgc ctggcgcaaa ggtatcaaaa cgctctatta
     4441 catccgcctg cgtcagatgg cgctggaagg cactgaaatt gaaggctgcg tctcctgtgc
     4501 actttaagga atatctatga aactctcacg tatcagcgcc atcaactgga acaagatatc
     4561 tgacgataaa gatctggagg tgtggaatcg cctgaccagc aatttctggc taccagaaaa
     4621 ggtgccgctg tcgaacgata ttcccgcctg gcagacatta actgtcgtag aacaacaact
     4681 gacgatgcgc gtttttactg gcctgacgct gctcgacacg ctgcaaaatg ttatcggcgc
     4741 gccttcgctg atgcccgatg cgctcacgcc tcatgaagag gcggtattat cgaatatcag
     4801 ctttatggaa gcggttcatg cccgctctta cagttcgatt ttctcgacgc tgtgccagac
     4861 caaagatgtc gatgccgcct acgcctggag tgaagaaaac gcaccgttgc agcgaaaagc
     4921 tcagattatt cagcaacatt atcgcggtga tgatccgctg aaaaagaaaa tcgccagtgt
     4981 gtttcttgaa tcttttttgt tctattccgg tttctggctg ccgatgtatt tttccagccg
     5041 cggaaagctg accaataccg cggacctgat ccgtctgatt atccgcgatg aagcagtcca
     5101 cggttactac ataggctata aatatcagaa aaacatggaa aagatatctc tgggacaacg
     5161 tgaagagttg aagagtttcg ccttcgattt gttgctggaa ctctacgaca acgagttgca
     5221 atacaccgat gagctgtacg ccgaaacccc gtgggctgac gatgtgaaag cgtttctctg
     5281 ttacaacacc aataaggctt tgatgaatct gggctacgaa ccgttatttc ccgcagaaat
     5341 ggcggaagtg aatccggcaa tcctcgccgc gctttcgccg aatgccgatg aaaatcacga
     5401 tttcttttcc ggttcaggct cctcttatgt gatggggaaa gcggttgaaa cagaagatga
     5461 agactggaat ttctgagggt gttattttca aaaatatcac tacccgcagc agggaaataa
     5521 ttcccgccaa atagcttttt atcacgcaaa tattctgtag tgatctacac tgatactctg
     5581 ttgcattatt cgcctgaaac tacaatattc aggcgttttt tcgctatcta tgacaaaaaa
     5641 tatcaacttt tctcgatttg ctctcagccc ttatatcacg gaaaattccg gcgatttgct
     5701 cgcatcaata ttcatgccac atttgccctc aggggttgcc tcagattctc agtatgttag
     5761 ggtagaaaaa agtgactatt cccattgggt aatatatcga cataaacaaa taaagaaatc
     5821 attctattgc atggccatta aattaaaaat taaaaatctt tataaaatat ttggtgagca
     5881 tccacagcga gcgttcaaat atatcgaaca aggactttca aaagaacaaa ttctggaaaa
     5941 aactgggcta tcgcttggcg taaaagacgc cagtctggcc attgaagaag gcgagatatt
     6001 tgtcatcatg ggattatccg gctcgggtaa atccacaatg gtacgccttc tcaatcgcct
     6061 gattgaaccc acccgcgggc aagtgctgat tgatggtgtg gatattgcca aaatatccga
     6121 cgccgaactc cgtgaggtgc gcagaaaaaa gattgcgatg gtcttccagt cctttgcctt
     6181 aatgccgcat atgaccgtgc tggacaatac tgcgttcggt atggaattgg ccggaattaa
     6241 tgccgaagaa cgccgggaaa aagcccttga tgcactgcgt caggtcgggc tggaaaatta
     6301 tgcccacagc tacccggatg aactctctgg cgggatgcgt caacgtgtgg gattagcccg
     6361 cgcgttagcg attaatccgg atatattatt aatggacgaa gccttctcgg cgctcgatcc
     6421 attaattcgc accgagatgc aggatgagct ggtaaaatta caggcgaaac atcagcgcac
     6481 cattgtcttt atttcccacg atcttgatga agccatgcgt attggcgacc gaattgccat
     6541 tatgcaaaat ggtgaagtgg tacaggtcgg cacaccggat gaaattctca ataatccggc
     6601 gaatgattat gtccgtacct tcttccgtgg cgttgatatt agtcaggtgt tcagtgcgaa
     6661 agatattgcc cgccggacac cgaatggctt aattcgtaaa actcctggct tcggcccacg
     6721 ttcggcactg aaattattgc aggatgaaga tcgcgaatat ggctacgtta tcgaacgcgg
     6781 taataagttt gtcggcgcag tctccatcga ttcgcttaaa accgcgttaa cgcagcagca
     6841 aggtcttgat gcggcgctga ttgatgcgcc gttagcagtc gatgcacaaa cgcctcttag
     6901 cgagttgctc tctcatgtcg gacaggcacc ctgtgcggtg cccgtggtcg acgaggacca
     6961 acagtatgtc ggcatcattt cgaaaggaat gctgctgcgc gctttagatc gtgagggggt
     7021 aaataatggc tgatcaaaat aatccgtggg ataccacgcc agcggcggac agtgccgcgc
     7081 aatccgcaga cgcctggggt acaccgacga ctgcaccggc tgacggcggt ggtgctgact
     7141 ggctgaccag tacgcctgcg ccaaacgtcg agcattttaa tattctcgat ccgttccata
     7201 aaacgctgat cccgctcgac agttgggtca ctgaagggat cgactgggtt gttacccatt
     7261 tccgtcccgt cttccagggc gtgcgcgttc cggttgatta tatcctcaac ggtttccagc
     7321 aattgctgct gggtatgcca gcgccagtgg cgattatcgt tttcgctctc atcacctggc
     7381 agatttccgg ggtcggaatg ggcgtggcga cgctggtctc gctgattgcc atcggcgcaa
     7441 tcggtgcctg gtcgcaggca atggtgactc tggcgctggt gttaactgcc ctgctgttct
     7501 gtatcgtcat cggtttgccg ttggggatat ggctggcgag aagtccgcga gcggcgaaaa
     7561 ttattcgtcc actgcttgat gccatgcaga ccacgccagc gtttgtttat ctggtgccaa
     7621 tcgtcatgct atttggtatc ggtaacgtgc cgggcgtggt ggtgacgatc atctttgcgc
     7681 tgccgccgat tatccgtctg accattctgg ggattaacca ggttccggcg gatctgattg
     7741 aagcctcgcg ctcattcggt gccagcccgc gccagatgct gttcaaagtt cagttaccgc
     7801 tggcgatgcc gaccattatg gcgggcgtta accagacgct gatgctggcc ctttctatgg
     7861 tggtcattgc ctcgatgatt gccgtcggcg ggctgggtca gatggtactt cgcggtatcg
     7921 gtcgtctgga tatggggctt gccaccgttg gcggcgtcgg gattgtgatc ctcgccatta
     7981 tcctcgatcg cctgacgcag gccgttgggc gcgactcacg cagtcgcggc aaccgtcgct
     8041 ggtacaccac tggccctgtt ggtctgctga cccgcccatt cattaagtaa ctctgcactt
     8101 gcccggtgac gccgggcatt atcaccctgc caaaaaaagg aataacaatg cgacatagcg
     8161 tactttttgc gacagcgttt gccacgctta tctctacaca aacttttgct gccgatctgc
     8221 cgggcaaagg cattactgtt aatccagttc agagcaccat cactgaagaa accttccaga
     8281 cgctgctggt cagtcgtgcg ctggagaaat taggttatac cgtcaacaaa cccagcgaag
     8341 tggattacaa cgttggctac acctcgcttg cttccggcga tgcaaccttc accgccgtga
     8401 actggacgcc actccatgac aacatgtacg aagctgccgg tggcgataag aaattttatc
     8461 gtgaaggggt atttgttaac ggcgcagcac agggttacct gatcgataag aaaaccgccg
     8521 accaatacaa aatcaccaac atcgcacaac tgaaagatcc gaagatcgcc aaactgttcg
     8581 ataccaacgg cgacggtaaa gcggatttaa ccggttgtaa ccctggctgg ggctgcgaag
     8641 gtgcgatcaa ccaccagctt gccgcgtatg aactgaccaa caccgtgacg cataatcagg
     8701 ggaactacgc agcgatgatg gccgacacca tcagtcgcta caaagagggc aaaccggtgt
     8761 tttattacac ctggacgccg tactgggtga gtaatgagct gaagccaggg aaagatgtgg
     8821 tctggttgca ggtgccgttc tccgcactgc cgggcgataa aaatgccgat accaaactgc
     8881 cgaatggcgc gaattacggc ttcccggtca gcaccatgca tatcgttgcc aacaaagcct
     8941 ggaccgagaa aaatccggcg gcagccaaac tgtttgccat tatgcagttg ccagtggccg
     9001 atattaacgc ccagaacgcc attatgcatg acggcaaagc ctcagaaggc gatattcagg
     9061 gccacgttga tggctggatc aaagcccacc agcagcagtt cgatggctgg gtgaatgagg
     9121 cgctggcagc gcagaagtaa tttttattcg ggcggataag gcgttttcgc ctcatccgcc
     9181 gctctgtaca caatgcctga tgcgacgctg acgcgtccta tcatgcctac aaaccacatc
     9241 gcacatttca acaagctatc tttcatccca tattcatcaa catccgctat tattgatttc
     9301 cagcttaatc atcacctgat gaacaaaaat aatgactaaa cctaatcatg agcttagccc
     9361 ggcgctgatc gtgctgatgt ctatcgccac cggtctggcg gtagccagta actattacgc
     9421 ccagccattg ctcgacacca tcgcgcgtaa cttttccctt tccgccagtt cggcaggctt
     9481 tattgttacc gccgcgcagt tgggctatgc cgcaggtcta ctgtttcttg ttcccctcgg
     9541 tgatatgttt gaacgccgcc gcctgattgt ctcgatgacc ttactggcag cgggcggtat
     9601 gttaatcact gccagcagtc agtcactggc gatgatgatc ctcggtacgg cattaaccgg
     9661 tttattctca gtcgtggcac aaattctggt tccgctggca gcgacgctgg cttcaccgga
     9721 taaacgcggc aaagtggttg gcaccattat gagcggtctg ctgttgggga tattgctggc
     9781 acgaaccgtt gccgggttac tggcaaacct cggcggctgg cgcaccgtct tttgggttgc
     9841 ttcggtgtta atggcactga tggcgctggc attatggcgt ggtctgccac aaatgaaatc
     9901 agaaacccac ctcaactacc cacagttgtt gggttccgtt ttcagcatgt tcatcagcga
     9961 taagatcctg cgcacccgcg cgttgctggg ctgcatgacc tttgccaact tcagcattct
    10021 ctggacctca atggcctttt tgcttgccgc tccacctttt aactacagcg atggtgacat
    10081 tggtctgttt gggcttgcgg gagctgccgg agcgttgggc gctcgtccgg cgggcggttt
    10141 tgccgacaag ggcaaatcgc accacaactt tcggtctgct tctgctgtta ctttcatggc
    10201 tggcaatctg gtttggtcac acttccgtac tggcgttgat tatcggcatt ctggtgctgg
    10261 acctcaccgt acagggcgtg catatcacta accagacggt aatttatcga atacatcctg
    10321 atgcgcgtaa tcgcctgacc gcaggttaca tgaccagcta ctttattggc ggtgccgccg
    10381 gttcgctaat ttcagcctca gcctggcaac atggcggttg ggctggcgtt tgtctggctg
    10441 gcgcgacgat tgccctggtt aacttactgg tctggtggcg aggttttcat cgtcaggaag
    10501 ccgcaaatta agcaatcagc aaccgttttc gtggctttac acttataagg gtgttaagaa
    10561 gcccatcagt ctgataaggt taagatattc attcagtcta tttataatat taacaatcgt
    10621 taagcgtaca ctctatggaa agccctactc cacagcctgc tcctggttcg gcgaccttca
    10681 tggaaggatg caaagacagt ttaccgattg ttattagtta tattccggtg gcctttgcgt
    10741 tcggtctgaa tgcgacccgt ctgggattct ctcctctcga aagcgttttt ttctcctgca
    10801 tcatttatgc aggcgcgagc cagttcgtca ttaccgcgat gctggcagcc gggagtagtt
    10861 tgtgggttgc tgcactgagc gtcatggcaa tggatgttcg ccatgttttg tatggcccgt
    10921 cactgcgtag ccgtattatt cagcgtctgc aaaaatcgaa aaccgcccta tgggcgtttg
    10981 gcctgacgga tgaggttttt gccgccgcca ccgcaaaact ggtacgcaat aatcgccgct
    11041 ggagcgagaa ctggatgatc ggcattgcct tcagttcatg gtcatcgtgg gtatttggta
    11101 cggtaatagg ggcattctcc ggcagcggct tgctgcaagg ttatcccgcc gttgaagctg
    11161 cattaggttt tatgcttccg gcactcttta tgagtttcct gctcgcctct ttccagcgca
    11221 aacaatctct ttgcgttacc gcagctttag ttggtgccct tgcaggcgta acgctatttt
    11281 ctattcccgt cgccattctg gcaggcattg tctgtggctg cctcactgcg ttaatccagg
    11341 cattctggca aggagcgccc gatgagctat gaggttctgc tgcttgggtt actggttggc
    11401 gcggcgaatt attgcttccg ctatttgccg ctgcgcctgc gtgtgggtaa tgcccgccca
    11461 accaaacgtg gcgcggtagg tattttgctc gacaccattg gcatcgcctc gatatgcgct
    11521 ctgctggttg tctctaccgc accagaagtg atgcacgata cacgccgttt cgtgcccacg
    11581 ctggtcggct tcgcggtact gggtgccagt ttctataaaa cacgcagcat tatcatccca
    11641 acactgctta gtgcgctggc ctatgggctc gcctggaaag tgatggcgat tatataaccc
    11701 acaagaatca tttttctaaa acaatacatt tactttattt gtcactgtcg ttactatatc
    11761 ggctgaaatt aatgaggtca tacccaaatg gatagttcgt ttacgcccat tgaacaaatg
    11821 ctaaaatttc gcgccagccg ccacgaagat tttccttatc aggagatcct tctgactcgt
    11881 ctttgcatgc acatgcaaag caagctgctg gagaaccgca ataaaatgct gaaggctcag
    11941 gggattaacg agacgttgtt tatggcgttg attacgctgg agtctcagga aaaccacagt
    12001 attcagcctt ctgaattaag ttgtgctctt ggatcatccc gtaccaacgc gacgcgtatt
    12061 gccgatgaac tggaaaaacg cggttggatc gaacgtcgta aaagcgataa cgatcgccgc
    12121 tgcctgcatc tgcaattaac ggaaaaaggt cacgagtttt tgcgcgaggt tttaccaccg
    12181 cagcataact gcctgcatca actctggtcc gcgctcagca caacagaaaa agatcagctc
    12241 gagcaaatca cccgcaaatt gctctcccgt ctcgaccaga tggaacaaga cggtgtggtt
    12301 ctcgaagcga tgagctaacg cgtcatctcg ctcaaaaatc cagatttata aaagaaaaaa
    12361 tgaccggcca gcatcgcaac atgctggcct ttttggcaag caggtcggct cagccgatga
    12421 gttaagaaga tcgtggagaa caatatgagc gcaaatgcgg agactcaaac cccgcagcaa
    12481 ccggtaaaga agagcggcaa acgtaagcgt ctgctcctcc ttctcacctt gctctttata
    12541 attattgccg tagcgatagg gatttattgg tttttggtac tgcgtcattt cgaagaaacc
    12601 gatgacgcat acgtggcagg gaatcagatt caaattatgt ctcaggtgtc tggcagcgtg
    12661 acgaaagtct gggccgataa caccgatttt gtaaaagaag gcgatgttct ggtcactctc
    12721 gacccgacag atgctcgcca ggcgtttgaa aaagccaaaa ctgcactggc ttccagcgtt
    12781 cgccaaaccc accagctgat gattaacagc aaacagttgc aggcgaatat tgaggtgcaa
    12841 aaaatcgccc tcgcgaaagc acaaagcgac tacaaccgcc gtgtgccgct gggcaatgcc
    12901 aacctgattg gtcgcgaaga gctgcaacac gcccgcgacg ccgtcaccag tgcccaggcg
    12961 caactggacg tcgctattca acaatacaat gccaatcagg cgatgattct ggggactaaa
    13021 ctggaagatc agcctgccgt gcaacaggct gccaccgaag tacgtaacgc ctagctggcg
    13081 ctggagcgta cccgtattgt cagtccgatg accggttatg tttcccgtcg tgccgtgcaa
    13141 ccgggtgcgc aaattagccc aacgacgccg ctgatggcgg tcgttccagc caccaatatg
    13201 tgggtggatg ccaactttaa agagacgcag attgccaata tgcgcatcgg tcagccggtc
    13261 accatcacca ccgatattta cggcgatgat gtgaaataca ccggtaaagt ggttggtctg
    13321 gatatgggca caggtagcgc gttctcactg cttccggcgc aaaatgcgac cggtaactgg
    13381 atcaaagtcg ttcagcgtct acctgtgcgt atcgaactgg accagaaaca gctggagcaa
    13441 tacccgctgc gcattggttt gtccacgctg gtgagcgtca ataccactaa ccgtgacggt
    13501 caggtactgg caaataaagt acgttcaaca ccggtcgcgg taagtaccgc gcgtgaaatc
    13561 agcctggcac ctgtcaataa actgatcgac gatatcgtaa aagctaacgc tggctaatcc
    13621 agaggtgcgt gtgatgcaac agcaaaaacc gctggaaggc gcgcaactgg tcattatgac
    13681 gattgcgctg tcactggcga cattcatgca ggtgctgaac tccaccattg ctaacgtggc
    13741 gatccccact atcgccggga atctgggctc atcgctcagc caaggaacgt gggtaatcac
    13801 ttctttcggg gtggcgaatg ccatctcgat cccgcttacc ggctggctgg caaagcgcgt
    13861 cggggaagtg aaactgttcc tttggtccac catcgccttt gctattgcgt cttgggcgtg
    13921 tggtgtctcc agcagcctga atatgctgat cttcttccgc gtgattcagg ggattgtcgc
    13981 cgggccgttg atcccgcttt cgcaaagtct attgctgaat aactacccgc cagccaaacg
    14041 ctcgatcgcg ctggcgttgt ggtcgatgac ggtgattgtc gcgccaattt gcggcccgat
    14101 cctcggcggt tatatcagcg ataattacca ctggggctgg atattcttca tcaacgtgcc
    14161 gattggcgtg gcggtggtgt tgatgacact gcaaaccctg cgcggacgtg aaacccgcac
    14221 cgaacggcgg cggattgatg ccgtggggct ggcactgctg gttattggta tcggcagcct
    14281 gcagattatg ctcgaccgcg gtaaagagct ggactgtttt tcatcacagg aaattatcat
    14341 tcttaccgtg gtggcggtgg tggctatctg cttcctgatt gtctgggagc tgaccgacga
    14401 taacccgata gtcgatctgt cgttgtttaa gtcgcgcaac ttcaccatcg gctgcttgtg
    14461 tatcagcctc gcgtatatgc tctacttcgg cgctattgtt ctgctgccgc agttgttgca
    14521 ggaggtctac ggttacacgg cgacctgggc aggtttggcc tctgcgccgg tagggattat
    14581 tccggtgatt ctgtcgccga ttatcggccg cttcgcgcat aaactggata tgcggcggct
    14641 ggtaaccttc agctttatta tgtatgccgt ctgcttctac tggcgtgcct atacctttga
    14701 accaggtatg gatttttgcg cgtcggcctg gccgcagttt atccaggggt ttgcggtggt
    14761 ctgcttcttt atgccgctga ccaccattac gctgtctggt ttgccaccgg aacgactggc
    14821 ggcggcatcg agcctctcta actttacgcg aacgctggcg gggtctatcg gcacgtcgat
    14881 aaccacgacc atgtggacca accgcgagtc gatgcaccat gcgcagttga ctgagtcggt
    14941 aaacccgttc aacccgaatg cccaggcgat gtacagtcaa ctggaagggc ttgggatgac
    15001 gcaacagcag gcatcaggct ggattgccca gcagatcacc aatcaggggc tgattatttc
    15061 cgccaatgag atcttctgga tgtcagccgg gatattcctc gtactgctgg ggctggtgtg
    15121 gtttgctaaa ccgccatttg gcgcaggtgg cggcggaggc ggtgcgcact aagtacaact
    15181 aagccagttc atttgaactg gcttttttca attaattgtg aagatagttt actgactaga
    15241 tgtgcagttc ctgcaacttc tctttcggca gtgccagttc ttcgttgctg ttgatgcgta
    15301 cctcacgttc cagaatgcta cgcgcaatat cctgcgcttc ctgcaacgag tgcatctggt
    15361 aagtgccaca ctggtagacg ttcagttccg ggatctgatt ctgatcctgc actttcagca
    15421 cgtcttccat tgccgctttc caggcatcag caacacgctg ctcatctggc gtaccaatca
    15481 gactcatata aaaaccggtg cggcagccca ttggcgagat atcgataatc tctacaccat
    15541 taccgttaag atggttacgc ataaaaccag caaacaggtg ctccagggta tggatccctc
    15601 tttctggcat cacttctttg ttcggcacgc agaagcgcag atcgaacacg gtgattgcgt
    15661 cgccatgcgg ggtgttcatt gttttcgcca cccgaactgc aggcgcttcc atccgggtat
    15721 gatcgactgt gaagctatct aacaacggca tttagccacc tccggtaatt tttttaaaaa
    15781 ttttctgaac tctttcttcc caggcgagtc tgagtatatg aaagacgcgc atttgttatc
    15841 atcatccctg aattcagaga tgaaattttg gccactcccg agtggccttt ttcttttctg
    15901 tcaggcgtgt ttttccagcc acaccgcaaa cggttcggta tcagcggttt ccatttcctg
    15961 ctgacggcgt tcagatgcct cgcgctcggc tacaaaatcc tcttcgcgca gaatttccag
    16021 cggctcttca cgcagcagat tacggtaggc ttctgcaaac gctttgcctg ttccgccaat
    16081 accagtatca atcatagacc ttaagatacg ggcagagaaa gtcagatcgg gattatcgaa
    16141 gcaggcaacc agttcatcac acactttctg atacgcttcg ccgccgttaa tgctatccag
    16201 cgtttgcgcg acgcgtttca gatcgcggaa cagatcttta cccacctgca gtaacgggaa
    16261 ctgcgcggtt tcgcagccga tacccagcgt caggcccggt ttgcgacctt caaggatcac
    16321 ccggttccag ttaacgcgcg tacaggcaag ttcgctactg ctcatttccg gtgcatcagc
    16381 cagcgcacac cagaccataa acaggtcgag gaatcgcacc tgctgttcat ctacaccaat
    16441 cggcgagaac gggttgatgt ccagcgaacg cacttcaata tattcaatgc cgccacgtaa
    16501 cagcgcatca gaaggtgact cgccgctgcg ggtaacgcgt tttggacgaa tcggcgcata
    16561 cagttcgttt tcaatctgca gcacgttgct attgatttgc agcctcttac cgtctttctc
    16621 aataccaatc ttcgcgtact cttccgatgg cgttttgatt gcctgtttaa ggcccgctac
    16681 gtactcgtaa agatcgttga aggtaatacc aagattgctt tgcgatttat tggtatagcc
    16741 gagatcgctc aaacgaagag aggtcgcata cggcaggtaa tacataccgc actcggtttt
    16801 ctcaaacggc agcgacgttg gttttccttg caggaaagaa gaacaaatcg ccggagatgc
    16861 accaaacaga taaggaataa cccaaccgaa acgatagtaa ttgcggataa cgcggaaata
    16921 gcccgcagaa attttctctt tggcatcagc gcccgagata tcaccgcact tcgcttgcca
    16981 gaatgccatt ggcaaagaga aattgtagtg cacgccggaa atggtttgca tcagcgcgcc
    17041 gtagcgattt ttcagccctt cacgatacag cgttttaaag cgtccggtgt tagaagtgcc
    17101 gtactgtgcc agttcgatgt cctgaccttc tgcgatgtag catggcatac ttaacggcca
    17161 catccgctca tcgcccatat tgcgcgccgt ataacgatgc agatcgcgca taaaggtcag
    17221 catatgttca atatcaccat ccactggtgt aatgaattcc agcaatgctt ccgcaaaatc
    17281 ggtagtaatc catttgtgcg tcagtgcgga acctaatgct tcaggatgac ctgttgttgc
    17341 cagtgtgcca tcagcattaa cacgcaaagt ttcgcgctcc agcccacgct gtatcccctt
    17401 taacgcctga ggatgttttt ccagccaggc cagcgcctgt gatacgtccg ggatcaaatt
    17461 gacctcccgc ctgtcaaaat cgttttaatt agcataactg taatggtgac catatgtgca
    17521 ggcctacaat tagtgccacc acatcatgcc ctgaacggtc gctgctgcaa ctgcaacata
    17581 gcgtaacgct ttaccaaggc acaaaaaaaa gattactggc ccccaggaga tgcgcatcca
    17641 tcccgctaac agacacagta aatcgccaac cacgggcatc cagcttaata atagcgtgac
    17701 tgcaccatag cgtttcagcc agccggtagc tttctcttgc cagcgcgatg ttttacgcaa
    17761 tggaaagaaa cgcccaagga taacgttagt taaccctcca aggctattac ccattgttgc
    17821 tgttaaaact aaaacccagg gatgactgat cccggaaagc aacattgcca ccagcacgac
    17881 ttcggagttg ccgggtaata gtgtagcgct gagaaaacta ctggcaaaca acgagaaaag
    17941 cgataacgct tcactcacag caagcgaaca tccacagcgt ccatgcctgc tgcacgggcc
    18001 gcctgaatac cgaaatcggc atcttcaaag accacacact gcgtcggttg cacgcccata
    18061 cgctgcgcgc acaacaaaaa tgtgtctggc gcgggtttat ggtgtttgac gtgatcagca
    18121 gcgacgacgg cgtcaaaata acggcgtaat cccaggtgcg ccagcaatgc ctcagcgatg
    18181 gcgctttcac tccccgttcc tacagccatt gggcgacgac catgccaact tttcaccact
    18241 tcaacaagag gaagcggctc gacactatcc agcagcatac ttcttactgc ttctgttttt
    18301 tcacgcgcta acgcatgcgg gtcgagatcg gcctgattca gctcaataat tgcctgagca
    18361 atacgccagg tgggcgatcc attaagcgca atcatcgcct gaatatcgta ctgaagaccg
    18421 tagtgcccta atacttcgcg ccacgcttta cggtgcgtag gcgccgtatc caggattgtg
    18481 ccatccatat caaaaattaa acctgcataa cgctcgtaca tggtcttctc gcaagtcgaa
    18541 aaatatgacg ctactttagc gtaattgctt gattttgtcg ctgatggcga aggagaagaa
    18601 aattcaggaa atggataaag tagttattta gaaatgagat atttttgaag gaaatttttt
    18661 ggagaa
//
LOCUS       DAZPAB010000068        18476 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZPAB010000068 DAZPAB010000000
VERSION     DAZPAB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18476
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.068"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            96..521
                     /gene="nudI"
                     /locus_tag="ACHUPG_002064"
     CDS             96..521
                     /gene="nudI"
                     /locus_tag="ACHUPG_002064"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR0861682.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(560..1162)
                     /gene="ais"
                     /locus_tag="ACHUPG_002065"
     CDS             complement(560..1162)
                     /gene="ais"
                     /locus_tag="ACHUPG_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR0861683.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            1470..2609
                     /gene="arnB"
                     /locus_tag="ACHUPG_002066"
     CDS             1470..2609
                     /gene="arnB"
                     /locus_tag="ACHUPG_002066"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR0861684.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            2613..3581
                     /gene="arnC"
                     /locus_tag="ACHUPG_002067"
     CDS             2613..3581
                     /gene="arnC"
                     /locus_tag="ACHUPG_002067"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR0861685.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            3581..5562
                     /gene="arnA"
                     /locus_tag="ACHUPG_002068"
                     /pseudo
     CDS             3581..5562
                     /gene="arnA"
                     /locus_tag="ACHUPG_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            5559..6449
                     /gene="arnD"
                     /locus_tag="ACHUPG_002069"
     CDS             5559..6449
                     /gene="arnD"
                     /locus_tag="ACHUPG_002069"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR0861686.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVECCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            6449..8101
                     /gene="arnT"
                     /locus_tag="ACHUPG_002070"
     CDS             6449..8101
                     /gene="arnT"
                     /locus_tag="ACHUPG_002070"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR0861687.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            8098..8433
                     /gene="arnE"
                     /locus_tag="ACHUPG_002071"
     CDS             8098..8433
                     /gene="arnE"
                     /locus_tag="ACHUPG_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR0861688.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            8433..8819
                     /gene="arnF"
                     /locus_tag="ACHUPG_002072"
     CDS             8433..8819
                     /gene="arnF"
                     /locus_tag="ACHUPG_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR0861689.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(8813..9085)
                     /gene="pmrD"
                     /locus_tag="ACHUPG_002073"
     CDS             complement(8813..9085)
                     /gene="pmrD"
                     /locus_tag="ACHUPG_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR0861690.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(9190..10545)
                     /gene="menE"
                     /locus_tag="ACHUPG_002074"
     CDS             complement(9190..10545)
                     /gene="menE"
                     /locus_tag="ACHUPG_002074"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR0861691.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(10542..11504)
                     /gene="menC"
                     /locus_tag="ACHUPG_002075"
     CDS             complement(10542..11504)
                     /gene="menC"
                     /locus_tag="ACHUPG_002075"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR0861692.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(11504..12361)
                     /gene="menB"
                     /locus_tag="ACHUPG_002076"
     CDS             complement(11504..12361)
                     /gene="menB"
                     /locus_tag="ACHUPG_002076"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR0861693.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(12376..13134)
                     /gene="menH"
                     /locus_tag="ACHUPG_002077"
     CDS             complement(12376..13134)
                     /gene="menH"
                     /locus_tag="ACHUPG_002077"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR0861694.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(13131..14801)
                     /gene="menD"
                     /locus_tag="ACHUPG_002078"
     CDS             complement(13131..14801)
                     /gene="menD"
                     /locus_tag="ACHUPG_002078"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR0861695.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(14890..16185)
                     /gene="menF"
                     /locus_tag="ACHUPG_002079"
     CDS             complement(14890..16185)
                     /gene="menF"
                     /locus_tag="ACHUPG_002079"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR0861696.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLLPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(16264..16569)
                     /gene="elaB"
                     /locus_tag="ACHUPG_002080"
     CDS             complement(16264..16569)
                     /gene="elaB"
                     /locus_tag="ACHUPG_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR0861697.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(16624..17085)
                     /locus_tag="ACHUPG_002081"
     CDS             complement(16624..17085)
                     /locus_tag="ACHUPG_002081"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0861698.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            17150..18067
                     /gene="rbn"
                     /locus_tag="ACHUPG_002082"
     CDS             17150..18067
                     /gene="rbn"
                     /locus_tag="ACHUPG_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR0861699.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            18258..18446
                     /locus_tag="ACHUPG_002083"
                     /pseudo
     CDS             18258..18446
                     /locus_tag="ACHUPG_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         4396 a         4911 c         4638 g         4531 t
ORIGIN      
        1 tgcagttatc attgcaaaag agattactgt cactttcaca tatttctgag ttaccgtatt
       61 ctcatggttt ctttttattt ataaggaata cccctgtgcg acaacggact attgtatgcc
      121 ctttgattca aaatgatggt gcttatttgc tgtgcaaaat ggccgacgat cgcggcgttt
      181 tccccggtca atgggcgctt tcgggtggcg gcgtggagtc tggcgaacga attgaagagg
      241 cactacgccg cgaaattcgc gaagaactgg gagaacagct gcttttgaca gaaatcacgc
      301 cgtggacctt cagcgatgat attcgcacca agacatatgc agatggtcgc aaggaagaga
      361 tttatatgat ttacctgatt tttgactgcg tttctgccaa ccgagaagtg aaaataaacg
      421 aagagtttca ggactacgcg tgggtaaaac ctgaagatct ggtgcattat gatttaaatg
      481 ttgccacccg aaaaacgtta cgtttgaaag gtcttcttta acaatgatga cagccatcac
      541 tggctgtcag attgaaaatt tagtggttaa cgaattcccc atccagataa actttgcctt
      601 tttccacatg catgactaaa ccatccagat aatcaggttt aaatatcgca tcacgcttat
      661 ttttagcaat atatgtcagg caatgattat gggtgaaaat aacgatattt ttatcaggcg
      721 ctttgctttg taagtcctta attgcactat aaatctcatt accgcactga agaagtcgtt
      781 tatctaccgt cagcttttta cccgcggaaa accaggtcgc cgactgaatg gtccggacgg
      841 tattactgga ataaagatcg aaatcagaga tgtcagcgct aaaagcgttg cccagttcac
      901 gggcatcctg ggtaccttta accgtaatac ctgttttatc tgacaagcat tgattggttg
      961 aacgatcgca acgttcagca tggcgaaaca aaacgacaac cgggtgctgc tgtgccagtc
     1021 tggccaatgt tttattgtcg atacgtggca aaccattgct actccaggcg gcatgagtac
     1081 ccagtccggc aattgcagcg agcgccagta aaatgatgaa atattttttt gacttcaatg
     1141 atgagcggca aaaagctaac acgtaaactc cacctataga caagcgcaac cagacaatta
     1201 ccgtgaaatt gagcgacatt tctggcgata attagcagtt ggtgtaatat taaaaatcct
     1261 acgatgtcgg caaaatgcct caaatttttg ccaaatgcaa agcctaaata agaaaaaata
     1321 taaaaatttc aatatttacg tctaatatta gtttcttaag gttaagttaa tattctatcc
     1381 ttaaaatttc gctccaaatg gcaaaatata cacaacactc tttatagcaa atataagtgg
     1441 acaggtattc aatggcggaa ggaaaagcaa tgtcagaatt tttgcctttt tcgcgaccag
     1501 caatgggcgt ggaggaactc gctgcagtta aagaggttct cgaatccggt tggatcacaa
     1561 ccggtccgaa gaatcaggcg cttgagcaag ccttttgcca gttgacggga aatcagcatg
     1621 ccatcgcggt cagttcagcc accgccggaa tgcatatcac gctaatggcg ttggaaattg
     1681 gcaagggcga tgaagtgatt acgccttccc tgacctgggt ttcaaccctc aatatgattt
     1741 ccttgctggg tgcaacgccg gtaatggtgg atgtcgaccg cgatacgctg atggtcacgc
     1801 ctgaagcgat cgaatcagcc attacgccac gcactaaagc catcattccg gtgcattatg
     1861 ccggtgcgcc agcagatatt gacgccattc gcgccattgg cgaacgttac ggcatcgcag
     1921 ttatcgaaga tgctgcccat gccgtcggta cgtattacaa agggcgacat attggcgcaa
     1981 aaggtaccgc tattttttca tttcatgcca ttaaaaatat tacctgtgct gaaggtggcc
     2041 tgattgtaac tgataatgaa aaccttgccc gccagctacg gatgctgaaa tttcacggtc
     2101 tgggtgtcga tgcctatgac agacaaacct ggggccgtgc accgcaggct gaagtcttaa
     2161 caccgggcta taagtacaat ctgaccgata ttaacgccgc gattgccctg acacagttag
     2221 tcaaattaga gcacctcaac acccgtcggc gcgaaattgc ccagcaatat cagcaagcac
     2281 tggcagctct cccctttcag ccattaagcc ttcccgcctg gccgcacgtt cacgcctggc
     2341 atctgtttat tattcgtgtc gatgaacaac gttgtggtat cagtcgcgat gcgttgatgg
     2401 aagcgttaaa agaaagaggc attggtaccg ggttacattt ccgcgccgct cacacacaaa
     2461 aatattatcg cgagcgtttt cccacgctgt cgttaccgaa taccgaatgg aatagcgaac
     2521 gcatctgttc gttgccgctg ttcccggata tgactaccgc cgatgccgac cgcgtcatca
     2581 cagcccttca gcaactcgca ggacaataag ccatgtttga aatccaccct gttaagaaag
     2641 tctcggtggt tattcccgtt tataacgagc aggaaagctt accggaatta atcaggcgca
     2701 ccaccacagc ctgtgaatcg ttggggaaag agtatgagat cctgctgatt gatgacggca
     2761 gtagcgataa ttccgcgcat atgctggtcg aagcctcaca agcggagaac agccatattg
     2821 tgtctatttt gattaaccgc aattacgggc aacattcagc gattatggcg ggattcagtc
     2881 acgttactgg cgacttaatt attacccttg atgccgatct ccagaatccg ccagaagaaa
     2941 tcccccgcct ggtggcaaaa gccgatgaag gttacgacgt ggtagggact gtacgccaga
     3001 accgccagga cagctggttt cgtaaaacag cttcgaagat gattaaccgg cttattcagc
     3061 gcaccactgg caaagcgatg ggtgactacg gttgtatgct gcgcgcctat cgccgtcata
     3121 ttgtcgatgc gatgttgcac tgccatgaac gcagcacctt tatcccgatt ctggcgaata
     3181 tcttcgcccg ccgtgcaatt gaaattccag tacatcatgc cgagcgtgag tttggtgaat
     3241 ccaaatacag ttttatgcgc ctgattaatt tgatgtacga cctggtgacc tgccttacca
     3301 caacgccgct acgtatgctg agtctgctcg gcagcattat tgcgattgga ggttttagca
     3361 ttgcggtgct gctggtgatt ttacgcctga ccttcggacc acaatgggcg gcagaaggcg
     3421 tctttatgct atttgccgtg ctgtttactt ttattggcgc tcagtttatc ggcatgggat
     3481 tactcggtga atatatcggc aggatctaca ccgatgtccg cgcccgcccc cgctattttg
     3541 ttcagcaagt tatccgtcca tccagcaagg aaaatgaata atgaaaaccg tcgtttttgc
     3601 ctaccacgat atgggatgcc tcggtattga agctctgctg gctgccggtt acgaaattag
     3661 cgccattttt acccataccg ataatcccgg tgaaaaagcc ttttatggtt cggtggctca
     3721 tctggcggcg gaaagagaca ttccggttta tgcgccggat aacgttaatc atccgctgtg
     3781 ggtggaacgc attgcccaac tgtcaccaga ggtgattttc tctttttatt atcgccatct
     3841 tatttgcgac gagatttttc agctcgctcc cgcaggtgca tttaatctgc acggttcgct
     3901 gttaccaaaa tatcgtggtc gcgcgccgct gaactgggtg ctggtgaacg gtgaaacgga
     3961 aacgggcgtt acattgcacc gaatggtaaa cgtgccgatg ctggggccat tgtggcgcaa
     4021 ctgcgcgttg ccattgcgcc agacgatatc gctattacgc tgcatcataa attgtgccat
     4081 gccgcgcgcc agctactgga acagacatta cccgccatta aacacggtaa tattctggaa
     4141 atcgcccagc gcgaaaacga agccacctgt tttggtcgca gaacgccgga tgacagtttc
     4201 cttgaatggc ataaaccggc atccgtactg cacaacatgg tacgtgccgt tgccgatccg
     4261 tggccgggtg ccttcagcta tgttggcaat cagaaattca ccgtctggtc gtcgcgtgtt
     4321 catcctcatg ccagcaaagc acagccgggg agcgtgattt ctgttgcgcc actgctgatt
     4381 gcctgtggcg atggcgcgct ggaaatcgtc accggacagg cgggcgacgg cattactatg
     4441 cagggctcgc aattagcgca gatgctgggc ctggtgcaag gttcacgctt gaatagccag
     4501 cctgcctgca ccgcccgacg ccgtacccgg gtactcatcc tcggggtgaa tggctttatt
     4561 ggcaaccatc tgacagaacg cctgctgcgc gaagatcatt atgaagttta cggtctggat
     4621 attggcagcg atgcgataag ccgttttctg aatcatccac attttcactt tgtcgaaggc
     4681 gatatcagta ttcattccga atggattgag tatcacgtca aaaaatgtga tgtcgtcttg
     4741 ccgctggtgg cgatagccac gccgattgaa tatacccgca acccgctgcg cgtatttgaa
     4801 ctcgattttg aagagaatct gcgcattatc cgctactgcg ttaagtaccg taagcgaatc
     4861 atcttcccgt caacttcaga agtttatggg atgtgtagcg ataaatactt cgatgaggac
     4921 cattctaatt taatcgtcgg cccggtgaat aaaccacgct ggatttattc ggtgtcaaaa
     4981 caattacttg atcgggtgat ctgggcctat ggcgaaaaag agggtttaca gttcaccctc
     5041 ttcctcccgt ttaactggat gggaccacga ctggataacc ttaatgcagc gcgaattggc
     5101 agctcccgcg ctattacgca actcattctc aatctggtag aaggttcacc aattaagcta
     5161 attgatggcg gaaaacaaaa acgctgcttt actgatatcc gggacggtat cgaggcgttg
     5221 taccacatta tcgaaaatgc gggaaatcgc tgcgacggcg aaattatcaa cattggcaat
     5281 cctgagaacg aagcgagcat tgaggaactg ggcgagatgc tgctggcgag cttcgaaaaa
     5341 catccgctgc gccatcattt cccaccgttt gcgggctttc gcgttgtcga aagtagctgc
     5401 tactacggca aaggatatca ggacgtagag catcgtaaac cgagcatccg caatgcccac
     5461 cgctgcctgg actgggagcc gaaaattgat atgcaggaaa ccatcgacga aacgctggat
     5521 ttcttcctgc gcaccgttga tcttacggat aaaccatcat gaccaaagta ggcttacgca
     5581 ttgatgtcga tgcctttcgt ggcacccgtg aaggcgtgcc gcgtctgctg gaaatcttga
     5641 gtaagcataa tattcaggcc agcatttttt tcagcgtcgg cccggacaat atgggccgcc
     5701 atctctggcg actggtgaag ccacagtttt tgtggaagat gctgcgctca aacgcggcat
     5761 cgctttatgg ctgggatatt ttactggcag gtacggcctg gccaggtaaa gagattggtc
     5821 atgccaatgc cgatatcatt cgtgaagcgg ctaaacatca cgaagtcggt ctgcacgcct
     5881 gggatcacca tgcctggcaa gcccgtagcg gtaactggga tcggcaaaca atgatcgacg
     5941 atattgcacg cggtcttcgc actctggaag agattatcgg tcaaccggta acctgttctg
     6001 ccgctgcggg ctggcgtgcc gaccagaagg tgatcgaagc aaaagaagcg ttccatttgc
     6061 gctacaacag cgattgtcgt ggggccatac cgttccgtcc actgcttgaa tcaggaaacc
     6121 ctggcactgc gcaaattccg gtgaccttac ccacctggga tgaagtgatt ggtcgggatg
     6181 tgaaagcaga agattttaac ggttggttac tcaaccgcat cctgcgagat aaaggcacgc
     6241 cggtttatac cattcatgca gaagttgaat gctgcgctta tcagcataat tttgtggatc
     6301 tcctcaaacg cgcagctcag gaaggcgtga cattttgccc tttaagcgaa ctgttatcag
     6361 agacgttgcc gctcggacaa gttgttcgcg gaaatattgc cggacgtgaa ggctggctgg
     6421 gttgccaaca aattgcgggt agtcgctgat gaaatcggta cgttacctta tcggcatctt
     6481 cgcgtttatt gcctgctatt acctgttacc gatcagcacg cgtctgctct ggcaacccga
     6541 tgaaacgcgt tatgcggaaa tcagtcgaga aatgctggca tccggcgact ggattgtgcc
     6601 ccatctgtta gggctacgtt atttcgaaaa acccattgcc ggatactgga ttaacagcat
     6661 tgggcaatgg ctatttggcg cgaataactt tggtgtgcgg gcaggcgtta tctttgcgac
     6721 cctgttaact gccgcgctgg tgacctggtt tactctgcgc ttatggcgcg ataaacgtct
     6781 ggctctactc gccacagtaa tttatctctc attgtttatt gtctatgcca tcggcactta
     6841 tgccgtgctc gatccgttta ttgcattctg gctggtggcg ggaatgtgca gcttctggct
     6901 ggcaatgcag gcacagacgt ggaaaggcaa aagcgcagga tttttactgc tgggaatcac
     6961 ctgcggcatg ggggtgatga ccaaaggttt tctcgccctt gccgtgccgg tattaagcgt
     7021 gctgccatgg gtagcgacgc aaaaacgctg gaaagatctc tttatttacg gctggctggc
     7081 ggttatcagt tgcgtactga cggttctccc ctggggactg gcgatagcgc agcgggagcc
     7141 tgacttctgg cactattttt tctgggttga gcatattcaa cgctttgcac tagatgatgc
     7201 ccaacataga gctccgttct ggtactacgt gccggtcatc attgccggta gcctgccgtg
     7261 gctgggatta ctccccggtg cactgtacac aggctggaaa aaccgcaagc attccgcaac
     7321 cgtctatttg ttgagctgga cgataatgcc gctgctgttt ttctccgtcg ctaaaggcaa
     7381 attgcccacc tatattcttt cttgctttgc acctctggca atgctgttgg cgcattacgc
     7441 gttgctggca gcaaaaaata atcctctggc gctgcggatt aatggctgga ttaacatcgc
     7501 ttttggcgtc actggcatta ttgccacatt tgtgatctcc ccctggggac caatgaacac
     7561 gccggtgtgg caaaccttcg agagctataa agtcttttgt gcctggtcga ttttttcgct
     7621 atgggcattt ttcggctggt acaccttaac aaacgtcgaa aagacctggt ctttcgccgc
     7681 gctttgcccg ctggggctgg cgttgctggt aggattttca attcctgaca gagtcatgga
     7741 aggaaaacat ccgcaatttt ttgtcgagat gacacaagaa tcacttcagc caagccgcta
     7801 tattcttact gatagcgtcg gtgttgccgc aggtctggca tggagcctgc aacgcgatga
     7861 catcatcatg tatcgccaga caggtgagtt gaaatacggc cttaattatc cggatgcgaa
     7921 agggagattt gtcagcggtg atgagttcgc aaactggctt aatcaacatc gtcaggaggg
     7981 gattattaca ctcgtgcttt cggttgaccg cgatgaagat atcaacagtc tcgccattcc
     8041 gcccgcagat gccatcgttc gtcaggagcg tctggtgctg attcagtatc gtcccaaatg
     8101 atctggctaa cattagtctt tgccagcttg cttagcgttg ccgggcagtt gtgtcagaaa
     8161 caggcaacct gctttgtggc gataaacaaa cggcgcaaac atatcgtgct gtggctggga
     8221 ctggcgctgg cttgtcttgg tcttgccatg atgctctggc tgctggtctt gcagaacgta
     8281 ccggtaggca ttgcctaccc gatgttaagt ctgaattttg tctgggtgac gctggctgca
     8341 gtaaaactgt ggcacgaacc ggtatcgccg cgtcactggt gtggggtggc gttcattatt
     8401 ggtggcattg tgatcctcgg gagtacggtg taatgtgcct gatatgggga ttattcagcg
     8461 tcattattgc ctcagttgcg caattaagcc tgggttttgc ggcaagtcat ctgccgccga
     8521 tgacgcactt atgggatttt atcgcggctc tgttagcttt tggcctggat gcccggatac
     8581 tgttactcgg gctgctgggt tatctgcttt ccgttttttg ttggtataaa acgctacata
     8641 aacttgcgct aagcaaagcc tatgccttgc tcagtatgag ttatgtgctg gtatggattg
     8701 cttcaatggt acttcccggc tgggaaggga ctttttcgct gaaagcacta ctgggagtag
     8761 cctgtattat gagcgggttg atgctgattt tcctgcccac gacaaaacaa cgttactgag
     8821 ttttccctgc cgctgtacac tgccgttccc actcatcggg gctataactg cttgccgaga
     8881 ggactttcac cgtatgcagc ttttcacgat taatacaata aagcgcattc tgcaaaggcg
     8941 agagtaaatc ccccactttc acggcgaagt cactcttcac ttcggcgatc atttttatcg
     9001 cgccgccagc atcgcagagc ataagtacat gtctgttatc ttgtttattg cagcacgatt
     9061 ttttgtgcca gccattccat tgcattatcc tgtttgctaa gagttttcac atcaatttac
     9121 agattaacag ataaactctt tatcaacggc aatggaatgg cagaaatgag aagtgaaacg
     9181 gagggttaat tattgctgac gttgcaccca ctcttttagc gcctgacgtg aaattttaat
     9241 accgccgttt ttcagctccg gcggtaaagt cagccagcgc accggttgtt gaaaacgagc
     9301 cagcttatct ttcacccatt cgccaagatc aacggtttgc tggtcatact ccaccaccgc
     9361 taccggccga tgaccaaact ccttgtcggc aacggaaacg ataaacacct gcaaaaccgc
     9421 aggatgtgca gcaataacac gctcgacttc ttccggctga ataccctcac cgccactgaa
     9481 gaacagattg tctaaacgtc cgacaatggt cagcttgcca ttatgcatct caccgcgatc
     9541 gcgcgtagcg taccagcctt cgtcattaac cagtgaaacc agttgcccgt tacgccagta
     9601 accttctgcc atactggcag cccgcagcca cacttcatta ttaacgattt tcacttcccg
     9661 acccggcagc ggcaaaccaa cgtctgccag gccgtcggct tctttcgcac ataccgtgga
     9721 ggcaaactcg gtcagaccat agccgcaaaa gcaacgaatc ccctgctcgc gcgcctgttc
     9781 cgtcaactcg accgggatag ccgcgccgcc aagtaacacc gctttcaggg aaacggaact
     9841 gcggttaacc agcaaacgcc agagttgtgt tggcaccagt gaagcgtgag tacagcctgc
     9901 cagcatttgc tccaatggct gtttatcacg caccgtcatc cgcgcaccag cgtataacca
     9961 gcgccacata attccctgac cggagacgtg aaacagcggt aaagagagca accagtcatc
    10021 gtgatcgcca aacggaatca gcgataacac accttgcgca ctggcaagat gggcctgata
    10081 agtatggaca gcggctttcg gcaaaccggt agaacccgag gtcaacgtca ttgagcacag
    10141 acgcgtcggc tgccacgcag cggcatgtac gccttcaacc agctgaatgt gcagcgacgt
    10201 taatgccgga aacgtgtttt ccccatccgg caccagcgca aattgcagcg tcagattggg
    10261 cagcaattct tcaagcaacg gttgcggcag ctgagggttc acgggcaaca cccgcgcccc
    10321 gcattgcagt aacgccagcc aggcgagcag cgtttgcggc gtattccacg cccgcaacat
    10381 cacgccgctg ccctcaacca ccccctgcgc cgcaaagccc gtagctaact catcgacgcg
    10441 agcacaaagc tcgcgccagt tgagttgctc gtcattaaga cgtaaggcga tggcttctcc
    10501 ccgcacttgc cgccagtgac gccacggcca gtcagagaag atcataacaa ctgctcctgt
    10561 acatcagcgt ccactaacgg cagtgggctc cccggccagc gacgaacctg ctgcgcctgc
    10621 atcagatcca gcgtgtccag tcccggaatg gtgtctggcg ttaaccaggc agcaatccgc
    10681 gccagttgcg ttagacctaa gctcgactca atggaagaac tgatcaccgc cgtcagcccc
    10741 agcgcatgcg ccgcctgtac ctgctcgcgt actttttcca gactgcccgt gagcgtgggt
    10801 ttgataacta ccgcgcgcac gccctcttca gccacaaagg caaaatccgg ctcgcgcagg
    10861 ctttcatccc aggcaatggc aatgccggtt tcacgggcaa acgctcgcga atcatcgcgg
    10921 gttttgcacg gctcttcgag aaacgcgatg cggtggcgat aatccgggtt aacatatttg
    10981 gcaaactgct gacctttcag cggtgtccag gcgcgatttg cgtcaagacg caaatgcaga
    11041 tccggaattg cctccagcaa cagattcacc accatgccgt cgcgcaccgc ttcgtacaat
    11101 cccactttga ccttcgccac tttctcgccc ggcatatcgg caagtttgag gatcagatcg
    11161 tccggatcgc cattacacag cggtgccgca cggtagttgg ctgcttgcgg caacgtatct
    11221 gtcagttctg ccaatgcaca gcttacgcca aaggccacgg aaggcatctg cggtagctcg
    11281 caatcgcctg ccagccagtt atttacccag gcaagcagca cgctttgcgc ctcttcccag
    11341 ttttcctggc tgaagcccgg cagtggggag atctcccccc acccttcgcg ctcgccctca
    11401 cgcaggcaaa cgtacagccc gtcgcgggtt tttaaccgcc tgtcgcgcag aaccaccccc
    11461 gcgtccatgg ggatctgcca gcggtatacc tgcgcgctac gcattacgga ttccgtttaa
    11521 atttgctgaa atcaggctga cgtttctggt tgaaagcgtt gcgaccttcc tgaccttctt
    11581 ccgtcatgta gaacagcatg gtggcgttgc ccgccagctc ctgcagcccc gcctgcccgt
    11641 cacagtcggc gttcagcgca gctttcaggc agcgcagcgc catcgggctg ttttgcagca
    11701 tttcgcggca ccaacggacg gtttcttttt ccagatccgc cagcggtacc acggtgttga
    11761 caaggcccat atccagcgcc tgttttgcgt cgtactgacg gcacaggaac cagatttctc
    11821 gcgctttttt ctgcccgacg atacgagcca tgtaggaagc gccccagccg ccgtcgaagg
    11881 aaccgacttt cgggccagtc tgaccgaaga tggcattatc tgctgcgata gtcaggtcgc
    11941 acatcatgtg cagaacgtga ccaccgccga tggagtagcc agccaccatc gcgacaaccg
    12001 gtttcggaca ggtacggatc tgacgctgga agtccagcac gttcaggtga tgtacgccgg
    12061 aatcatcttt atagccgccg taatcaccac gcactttctg gtcaccaccg gagcagaacg
    12121 ctttatcgcc tgcgccagtc agaatgatca cgccgatatt gtcgtcataa cgcgcatctg
    12181 ccagtgcctg gatcatctct tttaccgtca gaggacggaa ggcattgcgc acctgcggac
    12241 gattaatggt gatttttgcg ataccgtcgg tggatttttc ataacgaatg tcctcgaaac
    12301 cttcggagca gtcgtgccat tcaaccggtg cgtaaggcat tgcttcatca ggataaatca
    12361 tattgtgtcc tttagtcaga aacgcaagat ctgcgccaga cttgcgatta cgccagcggg
    12421 attttcccga tgcgcgttat gtccggcgcg aggaatgaca tggcagtcgg cagccagttc
    12481 cgccgccagg gcgcggaatt tgctgtcacg ttcaccacat aaataataaa acgcaaatgt
    12541 gcgggcgcta aggttagcac gtaaatcagg ctggacggcg agagaagtcg cctccagcat
    12601 ggcggcaagg gttgcgccat tattgttgct gcgcagcgcc accagctccc ggcgttgatc
    12661 gtcattgagc gaggcaaaaa caggctgttg ataccagtcg gcaaataccg ccgttaacgg
    12721 ttctgtgcga aaacgctgcg cccattggcg atcggaacgc tgacgttccg cacgttgttc
    12781 agcattttgc agccccggat gcccgccttc gacaataacc ccacaaagcc ccgccagccc
    12841 ctggcaagcc gccatcatcg ccacccgtcc gccaagcgag taccccacca gccagaagtc
    12901 aaggatgttg taactaacca aggttttacg cagtaagtcg gtgacatcat caaatccatc
    12961 gacgctaatc gccgccgaac caccatgacc taggagatca acatacaacc gtgagtagtc
    13021 ggcaaacgcc tcgcccactt cttgccattc gtggcaatcg ccggaaaaac cgtgcaaaaa
    13081 caccagccag ggtaaaccta gttttccgtg ttttgcctgc gcgtgcagga tcataaatgg
    13141 cttacctgcg ccagaagttg ctggagcgtt tgcgcaccat cggtgtcgtt aaccaccatt
    13201 tcaatcaccg tggtggttgg cgtgcgccag gcgtcggcaa atgccgtttc aagttcctgc
    13261 cagttttgcg gatgatgata tttcagctcg aacatcgcgg cggcgtgctc aaaatggacg
    13321 ttttgcggca tcagatagaa acgctcacgc tcgctttgcg gcgttggcaa cagcgagaaa
    13381 atttgcccgc cgttgttgtt caccacaatt aataccagcg gcgcagaaac ctgacgcaat
    13441 aacgccagcg cgttgagatc gtaaagcgcg gagagatcgc ccacaatcgc cagcgtcggt
    13501 ttgccgcttg cccgctgaac gcctgcggcg gtcgaaagca gtccgtcgat accgctggcc
    13561 cctcggttgc tgtacaccgg gtaacctgcc ggaagttgcg aaagcgcatc aatcagacgt
    13621 accaccaggc tgttaccaac aaacaattgc ccctgttcag gcagatagtc gctgatgcga
    13681 tgcgccagtt gcgcttcgcc aaacgcatca cggcgggcaa taaccgcctg cattgcctgt
    13741 tccgccaggc gcgggatttc aacgcaccag ggctggcgtt tttctgccgg atgcagctcc
    13801 agccagtcgg caatattggc aattaagcga cgtccgcgat ggtgtgccgg atcaagtcgc
    13861 ccttcaatgt catcaacaat ccagtactct tctggttcac agcttgcctg ccattgcagg
    13921 agccgtttcc ccgtcaggct acttcccagt tgcaccacaa tttgcgcctg ctgcagctcg
    13981 ctggtcgctt tggcattgcc taaccaaaga tcggcacacg gcagcggctg cccggtttgt
    14041 gacagcacat cgccaatcag cggccagcca agagtttgcg cccacagggc aacttttttg
    14101 ccctcttccg cactcatgcg cccggcaacc accacgccgc gcttttgtcg ccagaagaac
    14161 cagtcgcgct gtttttcact ttccagacga ggcgcttcac gcagccacgg tttgtcgtcc
    14221 tgccaccagt cacccagacg ctgttgccag ctaagcccgg tatcgtccat ttcgccatac
    14281 agcggttcag caaacgggca gttgatatgg actccccccg catgaagcgt accgagagcg
    14341 tggtcgatgg tagaaaccag ccaacgtgcg gggatatcct gggtcgggcg cggcaatgaa
    14401 atactgtgcg tggggtgaga ggcgaacatt cccggctggc gaattgcctg attcgcgccg
    14461 cagtcaatta gctccggcgg gcgatcggcg gttaagagaa tcagtttttc tccggttaac
    14521 ccggcttcaa tcagtgccgg atagagattt gccaccgccg tgccggaggt cacaatcacc
    14581 gccaccggct gcttgctgac tttcgccagc cccagcgcca gatgccccaa cccacgctca
    14641 tcgaaatggg tgtggtgaat gaatgcggaa ttctccgccg ccgctaacgt taacggtgta
    14701 gaacgcgagc ctggggcgat acagatgtgt ctgacgccgt gacgcgttaa tgcttccaga
    14761 atgaccgccg cccagcgtcg gttaaatgcg cttactgaca tgagtttgtc cggtatcaat
    14821 attgcggcta agtataagga gctacaaaaa tagattattg atatgaatcg gtaatgatgc
    14881 gactcattac tattccattt gtaataaagt acgcagccct gccgctttgt tgtcgatttc
    14941 ctgccactct tgctcggggt cggaaccacg gacaatgccc gcgccagcat ataatcgcac
    15001 gacattgccg ctaatttttg ctgagcgcag ggaaacgcag aattcgcttt gttgtaatga
    15061 gagatagccc gcagaaccgg cgtaccattc tcgggtgaac ggttcgtgac gggcgataaa
    15121 ctgtcgcgcc agatcgcgcg gtaagccagc aactgctgcc gttggcagca actgatgtaa
    15181 acagatcaca tcatccgctt tgttgagtga agtccagata cagcggcgaa gatgctgcac
    15241 tttacgcaga cgcagtacct gcggcggtaa aacatccagc gtctgggtat cggcctgtaa
    15301 tcgttggcag atatcttcca ccaccagcat gttctcgcgc tggtttttat catccgccat
    15361 cagccactct cctaactgct gcgcctgctt atcatcagga tgatttgcta ctgttcccgc
    15421 cagcgcttca gtacgcagcg ctttgtcacg ccgccgccat aaccgttccg gtgaagagcc
    15481 aagaaaagca ttttcgccat caaaggccat gtaaaaatgg tagcaattca gattcagtcg
    15541 acgactggca gccatcatcg ccgccgcgtt gaccggactt gcgaaatgca ggtcagttgc
    15601 ccgagcgagc accactttgt cgagctcacc ttcggcgatg gttttcgttg ccagttcgat
    15661 taattgcgtc cagcccgttt tgtccggcca gtgttgttct cgcgtggtgg ttaaatgtaa
    15721 cccaggcaag ggcttgatac tcaccagtgt ggcgataaat tcttttgcct gaatcgcatc
    15781 gtgctgaagg gagctttcgc tgaatagcgt cagccgcagc gtggctttac cgccacagcg
    15841 tcgccattcc aggcggggta aaagtaaatt gccctgcgac gggtcaaaag cattcagccc
    15901 ccaaatgcgt aagtcagcgt gttccgggtg ctggcgaaga aaacgttgtg cctggtccaa
    15961 cgacgtaaaa cgggtaatcg cgcccaggac ggcagcttct tcatcaccat tacgttgttg
    16021 ccagtagaat tgcgggtacg tttgctgact ggccagccag ctcaaggcat caaaagcgtc
    16081 tttgagaggg aaaggaatat cgataacccg aatgccgggt gtcgccggaa tctcttgcga
    16141 caaatggcgc agtagatttt ccagcgccgt agtaagtgat tgcacgcgga cctctccctg
    16201 ttaaaagcct cacattatac ggggtactac aaaaaaaatg cagtaccccg gtgtagggag
    16261 gttttaacgg cgtgccagca acagtcctag taccagcccg acggccgcgc ccacaccaat
    16321 tccttgccag ggtttttcgt ggacgtagtc atcagcacga taaacagcct gcttcgcccg
    16381 atagtaataa ctgtctgaag cctggctaac ccgttttttc acatcatcca gcgctttttc
    16441 tgcatgcgct ttcagctcaa catatttctg atcggcggga tcgccagagg agcggagcac
    16501 ctcttccagt gtttcactaa gcagcgtcag gtcgtcatcg atacgtgtat caccaaactg
    16561 attagacatt ctcgttctcc atttgcgtaa aacctgtcac taactataga caagagttgt
    16621 tgattacgcc tgaattacct cgcgtgccat cccaatgtgt gggataccat cttcttcata
    16681 gacctccgtc acagggataa aaccgaaact ctggtagaag ttttgcagat gcgcctgcgc
    16741 cccgaggtaa acaggcttat caggccagtg atgcgtacat gtttcgagtg ttttactcat
    16801 cagctgctgc cccacttttt caccacgcaa cgcttcactg acaatcaccc gacctataac
    16861 gaccggctca agatcatcat cacttttcag aatcctcgca tacgccacca gttcatcatt
    16921 tttccaaccg agaatatggc gattatcccc cgtcaaatca tcaccatcga tatcctgata
    16981 agggcaattc tgctcgacaa caaacaccgc gcaacgcagt tgtaataagg cataaagctg
    17041 ggaaaccgat aattcagagt gatgcagatc ttgccattca atcataatgc tctcctttta
    17101 gatgtatgcc ttatactagc cggaatattt tttgaaacgt gatgaactca tggaattaat
    17161 ttttttaggt acttcagccg gtgtgccaac ccgcacgcgc aatgtcacgg caatattgct
    17221 taacctgcaa catccgaccc agagcggact ttggttgttt gactgcggtg aaggcaccca
    17281 gcatcagcta ctgcataccg cctttaaccc tggaaaactg gacaagattt ttatcagtca
    17341 ccttcatggc gatcatcttt ttggtttacc cggcttgctg tgcagtcgtt ctatgtcagg
    17401 cattatccaa cccttaacga tttatggtcc gcaaggtatc cgtgaatttg tggaaaccgc
    17461 gctgcggatt agcggctcat ggaccgatta tccgctggaa attgtcgaaa ttggcgctgg
    17521 cgaaattctc gatgatggcc tgcgcaaagt aaccgcttat ccgctggaac acccactgga
    17581 atgttatggc tatcgtattg aagaacatga taaaccgggt gcattaaatg cccaggcatt
    17641 aaaagctgct ggcgtgccgc ctggcccact gtttcaggaa ttaaaagcgg gcaaaacaat
    17701 cacgctggaa gatggaaggc agattaacgg cgcagattac ttagctgctc cagtgccagg
    17761 taaagcgctc gctattttcg gcgataccgg cccctgcgat gccgcacttg acctggctaa
    17821 aggtgtcgat gtcatggtgc acgaagcgac gctggatata accatggaag ccaaagccaa
    17881 tagtcgcggc catagctcta cacgccaggc tgcgacacta gcccgtgagg ctggagtcgg
    17941 caagctaatc attacccacg tcagctcgcg ctatgatgac aaaggttgtc aacacctgtt
    18001 acgtgaatgc aggtcaattt tcccggcgac tgaactggcg aatgatttca ccgtgtttaa
    18061 cgtttaactc tgcttattat taaaaacagg gtgaaacttg ccctgttata gcaacccgcg
    18121 ctacatatat ccacccacac aggcatattg agcgattttt gatatttatt catctcctta
    18181 cgcactatat tcagagcaat aagttgcctc ttttcatctt gattcagcac aacctttgca
    18241 actaaaagga gcccatgatg gttacagttg tcagcaatta ttgtcaatta tctcaaaccc
    18301 aactcagtca gacatttgca gaaaaattta ctgtgaccga ggaattactg cagtctttaa
    18361 aaaaaacagc gttatccgga gatgaagaaa gtattgagtt actgcataat attgcgttag
    18421 gttatgatga atttggaaaa aaagctgggt aatgctgcca acttactgat ttagtg
//
LOCUS       DAZPAB010000069        18278 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZPAB010000069 DAZPAB010000000
VERSION     DAZPAB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.069"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            57..696
                     /locus_tag="ACHUPG_002084"
                     /pseudo
     CDS             57..696
                     /locus_tag="ACHUPG_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            799..1122
                     /locus_tag="ACHUPG_002085"
     CDS             799..1122
                     /locus_tag="ACHUPG_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HFR0861700.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1189..2646)
                     /gene="nuoN"
                     /locus_tag="ACHUPG_002086"
     CDS             complement(1189..2646)
                     /gene="nuoN"
                     /locus_tag="ACHUPG_002086"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR0861701.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2653..4182)
                     /gene="nuoM"
                     /locus_tag="ACHUPG_002087"
     CDS             complement(2653..4182)
                     /gene="nuoM"
                     /locus_tag="ACHUPG_002087"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR0861702.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4346..6187)
                     /gene="nuoL"
                     /locus_tag="ACHUPG_002088"
     CDS             complement(4346..6187)
                     /gene="nuoL"
                     /locus_tag="ACHUPG_002088"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR0861703.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6184..6486)
                     /gene="nuoK"
                     /locus_tag="ACHUPG_002089"
     CDS             complement(6184..6486)
                     /gene="nuoK"
                     /locus_tag="ACHUPG_002089"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR0861704.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6483..7037)
                     /gene="nuoJ"
                     /locus_tag="ACHUPG_002090"
     CDS             complement(6483..7037)
                     /gene="nuoJ"
                     /locus_tag="ACHUPG_002090"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR0861705.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7049..7591)
                     /gene="nuoI"
                     /locus_tag="ACHUPG_002091"
     CDS             complement(7049..7591)
                     /gene="nuoI"
                     /locus_tag="ACHUPG_002091"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR0861706.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7606..8583)
                     /gene="nuoH"
                     /locus_tag="ACHUPG_002092"
     CDS             complement(7606..8583)
                     /gene="nuoH"
                     /locus_tag="ACHUPG_002092"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR0861707.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8580..11306)
                     /gene="nuoG"
                     /locus_tag="ACHUPG_002093"
     CDS             complement(8580..11306)
                     /gene="nuoG"
                     /locus_tag="ACHUPG_002093"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR0861708.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11359..12696)
                     /gene="nuoF"
                     /locus_tag="ACHUPG_002094"
     CDS             complement(11359..12696)
                     /gene="nuoF"
                     /locus_tag="ACHUPG_002094"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR0861709.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12693..13193)
                     /gene="nuoE"
                     /locus_tag="ACHUPG_002095"
     CDS             complement(12693..13193)
                     /gene="nuoE"
                     /locus_tag="ACHUPG_002095"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR0861710.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13196..14998)
                     /gene="nuoC"
                     /locus_tag="ACHUPG_002096"
     CDS             complement(13196..14998)
                     /gene="nuoC"
                     /locus_tag="ACHUPG_002096"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR0861711.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15092..15754)
                     /gene="nuoB"
                     /locus_tag="ACHUPG_002097"
     CDS             complement(15092..15754)
                     /gene="nuoB"
                     /locus_tag="ACHUPG_002097"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR0861712.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15770..16213)
                     /gene="nuoA"
                     /locus_tag="ACHUPG_002098"
     CDS             complement(15770..16213)
                     /gene="nuoA"
                     /locus_tag="ACHUPG_002098"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR0861713.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16844..17782)
                     /gene="lrhA"
                     /locus_tag="ACHUPG_002099"
     CDS             complement(16844..17782)
                     /gene="lrhA"
                     /locus_tag="ACHUPG_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR0861714.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4583 a         5068 c         4680 g         3947 t
ORIGIN      
        1 gctcataatt cattataaat aataatgaaa tgacgccttt tattgataat ttacatatga
       61 aaaaattttc cttttgttat attatttctg ttttcactcc ctctgattat ttttaccaac
      121 cctgggtcac tgcattatcg ccaacgcccc gacacgtaag tcctgagcaa ttagaaaaaa
      181 cagttcgtta tcttacacaa actgtgcatc cacgtaatgc cggcaatatt gataatctga
      241 ataggtccgc agagtatata aaagagttct ttgtcagtag cggtgccaga gttaccgcgc
      301 agggcgtccc cgattggcta ttacgactct gcgcctggaa gccagaatta cccttacccg
      361 gcaatgtcct ggctttatcc cgatcgggga gactttattg ctgtggtcgg cagaatgcag
      421 gatatcaacg ccgttcgtca ggtaaaagcg gcattattgt catcccggga tttatctgtt
      481 tattctatga atgccccagg gtttattccc ggtattgatt tttctgatca cctcaattat
      541 tggcaacacg atattcccgc cgtaatgatt actgacaccg ctttttatcg taataaacaa
      601 taccacttgc ccggtgatac cgcagacaga ttgaattatc agaaaatggc tcaggtggta
      661 gatggtgtta taaccttgtt atacaacagt aaataataaa aatttctcga ggcgggtcca
      721 gaaacagcga catgtacatt ttattttccc tcgtctgttt taagatgcga aacataaaat
      781 tcaatggagg aaaaataaat gggaatgatt ggctattttg cagaaattga ctcagaaaag
      841 attaaccaac tgcttgagtc tactgaaaaa cctttgatgg ataatataca tgacacgctt
      901 tcaggattac gccgactgga atcactggat cgaagtgagc tgcgtaaaca gttttccatt
      961 aagcgactca atgaaatgga gatttatccg ggagtgacct tttcagaaga gttagagggg
     1021 caactctttg cctcgatcat gcttgatatg gaaaagctga ttagcgcgta tcgacgtatg
     1081 ctgcgacaag ggaatcatgc gctgacggtt attgttgggt gatggtgtca gagcgccaaa
     1141 aaaaaccgcc gaacatgtcg gcggctttct gacttacaaa ctaacagttt acatcagcgg
     1201 cattgctaac cgcacaatac taatcagcgg ttgtggccat acacccagca ccagtaccaa
     1261 cagtgcagaa atcagcacca cgataccgcc cgcgctgtac tgccagtttg acggtgcatc
     1321 gcgacccggt tgttccggtg cgtgcaggta caggctcacc gccacgcgca ggtagtagta
     1381 gaggccgatt gccgaaccga caaccacggc acccaccagc caccacaagt gtgcctggac
     1441 accgaccgcc agcacgtaga acttaccgat aaagcccagc gtcatcggga taccggccag
     1501 agacagcatc atcaccgtca tcactgccgc gaggatcgga cgatgccaga acagaccgcg
     1561 gtaagagaac agggaatcag catccgggcc acgatacggg ctggacatca ggctgaccac
     1621 gccgaacgcg ccgaggctgc tgaacagata accggccagg taaaccccta ccgcttccat
     1681 cgacatctcg ccggtttgca gcgcaatcag cgccaccagc agatagccga ggtgagagat
     1741 agatgagtaa ccgagcagac gtttgatgtt ggtctggctc aacgccatca ggttaccgaa
     1801 gatgatggag gcaaaggcga taatcgccag tactacgcga atcgcttcgc tgtcacccac
     1861 cggtgcgtag aggaacagac gcatcaccac accgaagata gcgattttgc tcgccgtcgc
     1921 caggaaagtg gaaaccggcg caggcgcgcc ctggtatacg tctggcgtcc acaggtggaa
     1981 cggcaccaga gagagtttga agccgaggcc aacaatcatc atgccgaaac ctgccagcaa
     2041 cagcggctcg ttgagcatac cgtcgccgag gtttttaccc agcgcgacaa acgacaggtc
     2101 gccagactgc gcatacacca gcgccatacc gaacagcagg aaagaagacg ctgcggcaga
     2161 aaggatggtg tatttgatac tggcttccag cgaacgtttc tggcggaaag cgtaaccgac
     2221 caggccaaac agcggcaaag agatcagttc aataccgagg aacagagacg ccagatggtt
     2281 tgcattcgcc agcaggatcc cgcccagcgc ggcaattaac accaacaggt agaactcatc
     2341 cttgttgtcg ttatagcctt caagccacgg gtaggcgaaa gtacaggtgg cgaggctcgc
     2401 caacaatacc agcccggtgt aaagcatggc gaaaccatca acgcgcatca gcggcgtaac
     2461 gtccatagcg cccgcctggc caacaaacca gagcgaaacc agcgccgcgt taagcccaat
     2521 aaccgagagc gtagcgttga ggaaatgatt gcgtcgccac gcaatggaga gcatcacaac
     2581 caccaccgtc aagccgacga tcagcaacgg tagcagtgcg atcaggtttt gtggagttat
     2641 tgtcatggcg atttacggcc ttgtagtagt aacggaatta acaaaccact gctggatatt
     2701 gccaatcgcg gagtgcgagg tatccagaat cggctgcgga tagaagccca gcagtaccag
     2761 cagcaccacc agcaacagga tcataaacag ctcacgcagc gacatccctg gcagttcctg
     2821 gctggcaatc tggcttttcg ctttaccgaa gtaagcgcga tgcaacatcg ccagcgaata
     2881 aacagatgca aagaccagcc caaaggtaga gataacggta atcaccggga caacctggaa
     2941 gctgccgaac agaatcataa attcgccgac gaagttaccg gtgccaggca tcccaagcgt
     3001 tgccaccgca aagaacagcg acagtgctgg cagccatttc atcttgctcc acagaccgcc
     3061 catcatgcgc atgtcgcggg tatggatacg ttcataaagc tgaccgcaga gaataaacag
     3121 acccgccgcc gacaagccgt gcgcaatcat ctggattacc gcgccctggt aggccaactg
     3181 gctgccggtg tagattgcaa tcagcacgaa gcccatgtgg gaaaccgagg tgtaggcgat
     3241 cagacgtttg atatcggtct gagcgaaggt catccacgca ccgtagaaga tgccgataac
     3301 acccagccac atagcgattg gcgcgaactc tgccgacgcg ttcgggaaca gcggcaggga
     3361 gaaacgcaac aaaccgtaag cggcggtttt cagcaagatc cccgcgaggt ccacggaacc
     3421 ggcggtcgga gcctgggagt gcgcatccgg cagccagcca tgcagcggaa ccaccggcat
     3481 tttgactgcg aaggcgatga agaagcccag catcaacaga tattccacac cactggacat
     3541 tggcgtattc agcagctctt catagttgaa ggtccagacg ccggtcgcat tgtagtgaac
     3601 aaaaaccagc gccaggatgg cgatcaacat caccagacca ctcgcctggg tgtaaatgaa
     3661 gaacttggtt gccgccgtga tacgcgtttt accgtcagag gctttatgcc cccacagtgc
     3721 gatcaggaag tacatcggca ccagcatcat ttcccagaag aagaagaaca ggaacatgtc
     3781 gatggcaagg aacacgccga taacgccgcc caggatccac atcaggttga ggtggaagaa
     3841 gccctgatat ttttcgatct ctttccacga acatagtacc gccagcacac cgagcagacc
     3901 ggtcagcacg accatcagca gcgacagacc gtcaatggcg agatgaatag agataccaaa
     3961 acgcgggatc cacggcatgt cgaattcaga ctgccactgc ggaattccgg cggattgcgt
     4021 cagtgaataa ccgccctgca accacagttg cagcgacagc gccagcgtca atcccatggt
     4081 gatcagcgcg atccagcgtg gcaccttgac gccaaagcgt tcggtctgcc agcacaggaa
     4141 gccgccaata aagggaatta atattagcca gggtagtaac atggcgatct ttattccttg
     4201 taaaagtccc gtcaggaccg gattttcaac gaattctcac gacagaaaat tctcttcgga
     4261 ctgggggtag tgtcggatgc gccgcgaagg cagcttatcc gacctacgtt cttatatgcc
     4321 caggggcaat cccacaatcc ttaactcaac gcagtaccat caacagtgcc agcaccacga
     4381 ccgcaccgat gctcatggat gccacatacc agcgcagata accgttctcg cttaacagca
     4441 gacctttacc tgcaaagcgg gaaagaaccg ccgggatgtt catcattgag ttcagcggat
     4501 cgcgtttcag caaccaggca atacccagga acggcttgac gaacactttg tcgtacagcc
     4561 agtcaaagcc ccaagcgttg taccaccagg tactcagcag gcggcccggc gcactgttgg
     4621 cgatggaggt caccagagaa cgtttaccca gccacagcca ggctgccagc agaatgccga
     4681 ccaccgcgac cacgccagag gtaatttcca gggtcaacat gctgccgtgc gccagttccg
     4741 tcgtttgcgg aagcacgccc tgcagcggcg gtacaatcag tgcgccaacg aaggtggaaa
     4801 ggatcagcag cacaatcagc ggcaggctgt gagttacccc tttcacggcg tgagcgtgaa
     4861 tttgttcttt tccgtggaag acgatgaaaa tcatacggaa ggtgtagagc gaggtcataa
     4921 acgcaccgac cagacctgcc accatcagat tgatatgacc attcgccatc gcacccgcga
     4981 ggatctcatc cttactgaag aagcccgcag tgaccagcgg cagtgccgac agtgctgcgc
     5041 cgcccaccag gaagcagaga taaaccagcg gaatagattt acgcagaccg cccatcttga
     5101 agatgttctg ttcgtgatgg caggccagaa tgacggaacc ggatgccagg aacagcagcg
     5161 ctttaaagaa cgcgtgagtc atcaagtgga aaatcgccgc atcccatgcc tgcacgccaa
     5221 gcgcgaggaa catgtagcca atctggctca tggtagagta agcgagaaca cgtttgatgt
     5281 cggtctgtac cagcgcggca aaaccggcca gcagcagcgt aaccgccccg acaataccca
     5341 ccagatgcag aacttccggc gtcatcagga acaggccgtg ggtacgggcg atcaggtaga
     5401 cacccgcggt taccatcgtt gcggcgtgga tcagcgcgga gacaggcgtc gggcccgcca
     5461 tcgcgtcagc aagccatgtc tgcaacggca actgcgcaga tttaccgacc gcaccgccca
     5521 gcagcatcag cgtcgcccac atcagcatgt tattgccgtc agcaaagtgc gctggtgcca
     5581 gttccaccat atcgcggaag ttcagggtgc ccagttcgtt gtaaagaatg aacagtgcga
     5641 aagcgaggaa aacgtcaccc acacgggtca cgacgaacgc tttcattgct gctgcgccat
     5701 tcttcggatc ggtgtaatag aaaccgatca gcagatagga gcacaggccc acgccttccc
     5761 agccgaggta catcagcagc aggttatcgg cgagaaccag aactaccatg ctggcgatga
     5821 acaggttggt gtaggcgaag aagcgagagt agccctcttc accgcgcatg taccaggagg
     5881 cgtacatgtg aataaggaaa cccacaccag tgaccaccga gagcatggtc agcgacaggc
     5941 cgtccagcac caggttaaaa ccgatgttaa agtcgcctac cgacatccac gtccacagcg
     6001 gctggctgta tgcctgctcg ccgttagcga agaaatcaac gccgataaag gcggttacca
     6061 gcgccgccag gcccacagag ccaacgccga cgatcgccga aacgttttca gaccagcgcc
     6121 cacgggagaa cgccagcagg acgaagccaa tcaatggcaa aataatggtt aaggcaagca
     6181 tgttcatccg cgcatctcac ttactgaatc gatgttcagg ttctggcgac gacggtgaag
     6241 ttgcagcagc agcgcaaggc cgatactcgc ttctgccgcc gcgaggctga tggcgagaat
     6301 gtacatcacc tgaccgtcgg tctgacccca gtagcttccg gcgaccacaa aggccagcgc
     6361 ggaggcgtta atcatgattt ccagaccaat cagcataaac agcagattgc gacggataac
     6421 cagaccggtt aagccaagaa cgaataagat tgccgcgagg atcagtccat gttgtaaggg
     6481 gatcatgcgt gctcctccgt ttttcttttc gcgctgtcgt ctttacgatt gctcagcact
     6541 tcacccgcac gctcttcacg accgacgtgg aaggccacaa ccagacccgc gagcagcagc
     6601 atagaagcca gttccaccgc cagtacgtaa ggcccgaaca gcgtaatacc cactgcttta
     6661 gcactgattg gcgtaccgtc gataccctga tcgttaacac cgaggatggc gtaaacaatc
     6721 accaccagca tgatggccga caaaattgcc gggccaatcc acacctgcgg tttcagccac
     6781 tggcgttcct gttcgatttc tgaaccgccc aggttgagca tcatcaccac gaacacgaac
     6841 agcaccataa tggcacccgc gtagacgata atttccagcg caccggcgaa gtaagcgcct
     6901 agtgagaaga acaccccgga aatcgccagc agcgaaataa tcaggtacag cagtgcgtgt
     6961 accggattgg tatgggtgat cactcgcaag gtcgcaagta tggctatcag gccacagata
     7021 taaaaagcga actccattgc ccctctcctt acggtaacag gctcttgacg tcgataggct
     7081 tggcttcgtt ctctgcttcg cccttatctt tgccgtcgat tgccatacct gccatccggt
     7141 agaagttata ttccgggtat ttgcccggac cggagatcag cagatcctct ttctcgtaaa
     7201 ccagatcctg gcgcttgtat tcccccattt cgaaatccgg ggttaactga atcgccgtgg
     7261 tcggacaggc ttcttcgcac agaccacaga aaatgcagcg tgagaagtta atgcggaaaa
     7321 attccggata ccagcgaccg tctttggttt ctgctttttg cagcgagata cagccgaccg
     7381 ggcaggctac cgcacagagg ttacaggcta cgcaacgctc ttcgccgtcc gggtcgcggg
     7441 tcagaacgat acgaccacga taacggggcg gcagatagac cggctcttcc gggtacattc
     7501 gcgtttcgcg tttggcgaac gcgtgcaggc cgatcatcca gatactacga acctgggtgc
     7561 cgaaacctac taacaattct tttaaggtca tggtcttatt gccccttatt gcgcctgcca
     7621 gagaatgaca gccgccgtta ccagcaagtt gatcagcgtc agcggcaggc agattttcca
     7681 gccgaaggac attacccggt cataacgcgg acgcggtaac gacgcacgaa tcaaaatgaa
     7741 catcatcata aagaacgcgg ttttcagcgc gaaccagatg aatggcggta acaacgggcc
     7801 ttgccagcca ccgaagaaca gcgtcaccat caatgcagag atggtcacaa tcccgatgta
     7861 ctcacccacg aagaacagac cgaacttcat accggaatat tcaatgtggt aaccatccgc
     7921 cagttcctgc tcggcttccg gctggtcaaa cgggtgacgg tgacacaccg ccacgcccgc
     7981 gatggcaaag gtaataaaac caaagaattg cgggatgacg ttccacacat gcgcctggct
     8041 gttgacgatg tcggtcatgt tgaatgaacc ggcctgcgcc accacgccca tcagggaaag
     8101 cccgaggaac acttcgtagc tcagggtctg cgcagaagca cgcatcgcac ccagcagcga
     8161 gtatttgttg ttacttgacc agcccgcaaa cagcaccgcg taaaccgcca gacctgccat
     8221 catcaggaag aacaaaatcc cgatgttcag gtcggcaacc acccaacccg gactgactgg
     8281 cacaattgca aaggccagca gcagcgaggt aaaggcaatc atcggtgcca gggtaaagat
     8341 gacgcgatcc gagaatttcg ggatccagtc ttctttaaag aacattttga tcatgtccgc
     8401 aaccagctgg agcgaaccgc cccagccaac acggttaggt ccataacggt tctggaacag
     8461 acccagcagg cgacgttcgc caaagctcat gaatgcccca caggtgacaa ccaccagcag
     8521 gatcaccacc gctttgagga tggtcagcag gatctcaatc agttccggtg atatccaact
     8581 cattgttgtg cctccttgag atcctcaaga tgcgcgccag ccagcaccgg agcaatgccg
     8641 gacataccca tcggcaagcc cacctgccct gccgtcagtc cttcggcgat ttcaaccggc
     8701 agcgtgaccg tgttgccatc gtaactaaag gagacgcgtg tacctgcgtt cacacccaac
     8761 ttcgcggcat ccgctgggtt gagtttgatg tacggctgcg gcatacggct ctggaagacc
     8821 ggagcacgct gtgacaattc atcgctgcca aacaggtggt agtacggcgc gatacgccat
     8881 ttcccgtcct gcggctggaa gcgtgccggt acgctggtga agtaatccag accgttttcg
     8941 ctggtttcaa acagacgcac gcccggatcg ccaaagcgca gtttgccgcc cacttcgtcc
     9001 tggaatttgt tccacgcctg cggggagttc cagcccggcg cccaggcaaa cggcacttgc
     9061 gaacggtgcg cggtcggctg gttgttacct tccatcgaga aggtgaacat ggtgtcaata
     9121 tcctgcggct gacgcggctc atgaacgctg atattggcgc gcatggcggt acgaccgctg
     9181 taacggtgcg gttcacgggc cagtttctgc ccacgaatac ggaatgtcgc atccggcgca
     9241 gcatctttga tacccgccag ttctgggatt ttcgccacaa cagcgtcaat tacatggtcg
     9301 agctgcgtcc agtccacttc acggctcagc aaggtgctgt gcagcgagtg cagccagcgc
     9361 cagctttcca gcatgacagt tttgctgtcg taataagccg gatcgtaaac ctggaagaaa
     9421 cgttgggcgc gaccttcatt attaattacc gtaccgtcgc tttcagcaaa gctggcagag
     9481 gaaagcacca gatgggcgtt ttccatgatc gctgtgcgtt gatgatcaac caccatcacc
     9541 agcggcgctt tagccagcgc agcattcacg cgggtagcag aagcgtaacg atgcagatcg
     9601 ttttccaaca ccaccaccgc gtcggcgcgt ccggtttcca gttcggttaa cgcttcttca
     9661 agcgaaccgc cacccataat gcccagcccc atgctgttga cggaacgggc aatcatggtg
     9721 ataccaacgt cagcgccgcg acctttcagg gctttcgcga cgttagccgc agcctgaatc
     9781 acctctatgc taccggcgtt cgtcccggag ataatcaacg gtttcttcgc acctgccagt
     9841 gcctgcacga tgacgtcgat tttgttttgc agctcaggtt cgataccgtc aaccgctggt
     9901 gcggagttat ccagcgcatg ggcaatggca aaacctaaac gcgcctgatc ttcaaccggt
     9961 gcgcggtaag tccacgccgc gatatcatcc agacgagtgt catcaacgtt ggtaacaaac
    10021 agcggatgct tcgcacgttg accgatgttg aggattgccg caatctgcca gtcagccact
    10081 ttctgtgctg cagccatttc gcgcgcttta cctttcaccg cctgacgcac tgccagtgcg
    10141 acgcgcgcgc cggtctgggt aacgtcttcg cccagcacca gtaccgcatc gtaagattcg
    10201 atttcgcgca gagccggagt ataaatgccg ccttcacgca gcactttcag cgccagttgc
    10261 agacgttcct gctcaccgtg agcgataccg gtgtagaagt tttcttcgcc caccagttca
    10321 cgcagcgcaa agttgctttc cacgctggca cgcggagaac caataccgat cactttcttc
    10381 gactgacgca gaatatctgc cgcgccctgc attgcctgtt cggcgttgag ggtaatgaaa
    10441 tcatcgccac gacgctgtac tggctgacgc ggacgatcct tcaggttgac gtaaccgtaa
    10501 ccgaaacgac cacggtcgca gaggaagtag tggtttaccg taccgttgta acggttttcg
    10561 atacgacgca gttcgccgta acgttcaccg gggctgatgt tacagccgat ggaacattgc
    10621 tggcagatgc tcggcgcaaa ctgcatatcc catttacggt tgtaacgctc ggagtgcgtt
    10681 ttgtcggtaa atacgccggt cgggcaaatt tcgaccaggt taccggaaaa ttcgctttcc
    10741 agcgtgccgt cttccgggcg accgaagtag acgttgtcgt gcgcgccata aacgcccaga
    10801 tctgtaccgt cagcgtaatc tttgtagtaa cgcacacagc ggtagcaggc gatgcagcgg
    10861 ttcatttcgt gagagatgaa tggccccaaa tcctgattac ggtgggtacg tttggtgaaa
    10921 cggtagcgac ggaagctgtg tccggtcatc acggtcatat cctgaagatg gcagttaccg
    10981 ccttcttcac ataccggaca gtcgtgcggg tggttggtca ttaaccactc gaccacactt
    11041 tcacggaact gtttcgcttc ttcgtcgtca atggaaataa aggtgccatc ggaagccggt
    11101 gtcatacagg acatcaccag gcgaccacgc gtgtcttccg cgttttggta ttgcttcacc
    11161 gcacactggc ggcaagcacc gacgcttccc agcgccggat gccagcaaaa gtaaggaata
    11221 tcaaggccca gagacagaca agcttccagc aggttgtccg ctccgttgac ctcgtattct
    11281 ttgccgtcta catgaattgt agccattagc atgcttccag ttttctcggt cagggaccga
    11341 gcgttaatcg aaattcggtt accagcgctc tttcagcagg ttcggctgaa tcccattaat
    11401 caaatgggta ttgctgaacg gctgtttgat tcccgcctca aattcttcgc ggaaatattt
    11461 gatggcgctc tgtaacggct ccactgcacc aggtgcgtgg gcacagaaag ttttacccgg
    11521 gcctaagaat cgacacagtt gctcaagtgt ttcgatatcg cccggctggc cttcgccacg
    11581 ctccagcgca cgcagaattt tcacgctcca cggcagaccg tcgcggcacg gcgtacacca
    11641 gccgcaggac tcacgggcga aaaactcttc caggttacgc accagcgaca ccatgttgat
    11701 ctcatggtca accgccatcg ccagcgccgt acccagacgg ctgcccgctt taccgatact
    11761 ttcgaattcc atcggcagat caaggtgcgc ttcggtcagg aagtcagtcc ccgcgccgcc
    11821 tggctgccag gctttaaatt tcagaccatc acgcatacca ccggcgtaat cttcgaggat
    11881 ctcgcgtgcg gtggtgccga acggcagttc ccacagtccc ggatttttca cccgaccgga
    11941 gaagcccatc agcttggtgc cagcatcttt acttttcgag atgttctgat accactccac
    12001 gccgttagcg aggatcgccg gaacgttaca cagggtttcg acgttgttga cacaggtcgg
    12061 tttgccccat acgccggagg ttgccgggaa ggggggtttc gagcgtgggt tagcacgacg
    12121 cccttccagg gagttgatta acgctgtttc ttccccgcag atgtagcgcc ctgccccggt
    12181 atggacgaac agttcgaagt cgaaacctgt tcccataatg tttttgccaa gcaagcccgc
    12241 ttcagtggct tcggcaatgg cgcggcgcag atttactgcc gcttcgatat attcgccacg
    12301 caggaagatg tagccacggt aagctttcag cgcaaacgcg gagatgagca taccttccac
    12361 cagcaggtgc ggcagttgct ccatcaacag gcggtcttta taggtgcccg gctccatttc
    12421 atcggcgtta cacagcaggt aacggatgtt catggattcg tctttcggca tcaggctcca
    12481 cttcaggcca gtcgaaaagc ccgcgccgcc gcgccctttc agaccagcgt cttttacctg
    12541 attaacgatt tcgtccggag acagcccggt cagcgcctta cgcgcccctt cgtaaccgtt
    12601 tttgctgcgg tattcgtcca gccacactgg ctgtttgtca tcgcgcagac gccaggtcag
    12661 cggatgcgtt tcgggagtac ggataatgtt tttcatttat accgctccag cagttcaggg
    12721 atcgcttccg gggtcagatg cgcgtgagtg tcctcatcga tcatcatgtt tggcccttta
    12781 tcacagttcc ccaggcagca agttggcagc agcgtaaagc ggccatcaaa tgtcgtttgc
    12841 cctggtttga tgttcagctt tttctcgagc gccgcctgaa taccctgata cccgttgata
    12901 tgacagacca cgctgtcgca ataacggatc acatggcgac caaccggctg gcggaagatc
    12961 tgactgtaga acgttgccac accttcgacg tcgcttgccg gaatacccag cacatcggcg
    13021 atcgcgtgga tcgcaccatc cggcacccag ccacgctgct tctgaacgat tttcagcgct
    13081 tcaatggacg ccgcacgcgg gtcttcgtag tggtgcatct cgtgctcaat cgcttcacgc
    13141 tctgccgcac tcagctcaaa agcctcggtt tgtggttgtt gattctcgtg cataattagc
    13201 ggtccacatc tgacataaca aaatcgatac tgcccagata aacaatcagg tcagacacca
    13261 ggctgccgcg gatcgccgcc ggaatttgct gcaaatgcgc ataactcggc gtgcggatac
    13321 gagtgcgata actcatggtg ctaccgtcgc tggtcaggta gtaactgttg atccccttgg
    13381 tcgcctcaat catctggaaa gattcattgg caggcatcac cggcccccac gacacttgca
    13441 ggaagtgggt gatcagggtt tcgatatgtt gcagcgtgcg ctctttcggc ggcggcgtgg
    13501 tcagcgggtg atccgctttg aacgggcctt ccggcatgtt gttgaggcac tgctcaagaa
    13561 tgcgcagact ctggcgcagc tcttccactt tcagcattac gcgggtgtag cagtcagaaa
    13621 caccaccacc aaccgggatt tcaaagtcga agttttcata gccagaataa ggacgcgcct
    13681 tacgcacgtc gaagtcgatc ccggtagcac gcaggcccgc gccagtggtg ccccactcca
    13741 gcgcctcttt cgcgccatag gcggcaacgc cctgggaacg acctttcaga atggtatttt
    13801 gcagcgccgc tttctcgtaa gacgccagac gtttcggcat ccagtcgagg aactcacgca
    13861 gcagacgatc ccagccgcgc ggcaggtcgt gcgctacgcc gccaatacgg aaccacgccg
    13921 ggtgcatacg gaaacccgtg atcgcttcca ccagatcgta aattttctga cgatcggtaa
    13981 aggcgaagaa cacgggcgtc attgcgccga cgtcctgaat aaaggtcgag atgtacagca
    14041 ggtggctgtt gatacggaac agttcggaga gcataacgcg aatgacgtta acgcgatccg
    14101 gcacggtgat cccggccagt ttctctaccg ccagcacgta aggcatttcg ttaacgcagc
    14161 cgccgaggta ttcaatacgg tcggtatacg gaatgtagct gtgccaggac tggcgctcgc
    14221 ccattttctc cgcaccacgg tggtggtagc cgatgtctgg tacgcagtcg acaatctctt
    14281 cgccgtcgag ttgtaaaacg atacggaaag ccccgtgcgc cgacgggtgg ttcggaccga
    14341 ggttgaggaa catgaagtcc tcattttcgg tgccgcgttt catcccccac tcttccggtt
    14401 tgaaggtcag ggcttccatc tccagatcct gtttggcttt ggtcagctca aacggcgaga
    14461 attcggtagc gcgtgccggg taatctttac gcagcgggtg acctttccag gtttgcggca
    14521 tcatgatgcg gcgcaggttc gggtgaccgt cgaaagtaat gccaaacaga tcccaggttt
    14581 cacgctcata ccagttagcg ttcgggaaca gtttggtgaa ggtcggtacg tgcaggtcgt
    14641 tttctgccag cgccaccttc agcatgatgt cgcggttacg atcgatagaa atcagatggt
    14701 agaaaacgga aaaatccgcg gcaggtaacc cttcgcggtg tgtgcgcaga cgttcgtcca
    14761 tgccgtgtaa gtcaaacagc atgacgtaag gtttcggcag tttctttaag aaatcgccaa
    14821 cttccagtaa ttgttcacgc ttgatccaca caacgggaac cccggtgcga gtcgcctgaa
    14881 cagtaaaggc atccggccca aaacggttgc gcagttcgcc aattaccgga tcatcaagat
    14941 gatcgcgggt ctgccaggcg ggttcttgcg cggttaagtc agtcatattg ttcaccattg
    15001 caaatggtcc gtggtgactg tcggcgcagt aagcttcgcg aaatagattt aggaataagc
    15061 gaagaaaaat ccctttgccg acaggcgcaa attaaatctc gtcaggtgta cgcaggttgg
    15121 ttacggcaat gcgttcaccg cgcttgcgtt cgcgctctga ttgcatattg gcgcgataaa
    15181 cgccctgatc gccaaccacc caggagagcg gacgacgttc tttgccgata gattcctgca
    15241 acagcatcag tgcctgcatg tacgcttcag gacgcggcgg gcagcccggg atatacacat
    15301 caaccgggat gaatttatcg acgccctgca caacggaata aatatcgtac ataccaccag
    15361 agttggcaca ggcacccatt gagataaccc attttggttc cagcatctgg tcatacagac
    15421 gctgaataac cggtgccatt ttggtaaagc aggttcctgc aaccaccatc aggtcagcct
    15481 gacgcggcga agcacgcaat acttctgcgc caaaacgcgc cacgtcatgc accgcggtaa
    15541 acgaagtcac catctcaacg taacagcagg aaagaccgaa gttatacggc caaattgagt
    15601 ttttacgacc ccagttaacc atgtcattga gcttgcccat aaacacgttt ttgttaactt
    15661 cttgctccag agggtcggtt acgatctcct gcttttgcag ggggtaacgg tcgttctcac
    15721 cgttgggatc tatgcgggtg agcgtataat ccatcttaat gcctcgcggt tagcgttgac
    15781 gattagcgat actgttcgtt tccgggttca tacgctcgcg gcgtgaacgc gcgggcgtcc
    15841 agtccagcgc gccaatacgc accagataaa ccagacctgc cagtaacaca aaaataaaaa
    15901 ttgcagcttc cacaaagcct acccagccgc tttcgcggat agaggttgac catgcgaaca
    15961 gatacagcgc ttcaacgtcg aagataacga agaacatggc caccagataa aacttggcgg
    16021 acaggcgtaa gcgggcggag ccgaccgagt cgataccgga ttcaaacggc acgtttttcg
    16081 acctcgcgcg tgcgcgaccg cctaaaaacc aaccgcctac cagcatcagg caacacaggc
    16141 caatggcaac gataagaaag atagcgaatg cccagtgatg agcgatgact tcagtggatg
    16201 ttgacatact cattgcttac tcatcaaaag tagcgccaga ttcactgctc ttcacggcag
    16261 atggacgcca catcgattca tggggaggaa taaaaaaaac cttacaataa ctgtagaaat
    16321 tcttttatac agctaattga tgtggtcttt tactcctttc tataaccttt tgtcaacttt
    16381 aacaaaagct tcttcacatt agtttacata atatcaacac cattagcatt taatgccctt
    16441 tcaccccaga tccttgacga ctccaggata attagatgtt gttgaatcgt gtccgttgtg
    16501 aagcaatgga aaaaatacgg gtctattttg acaggaattt gtgtcgattc ctccccccaa
    16561 aagagagtat tttcttgatc tgtgacacgc ttttgttatt ccataacaaa aacgcagcaa
    16621 caaatttacg tattttttaa catcattgta gcaggtgatt tttttcaggc gattatttgt
    16681 gcattcggga cgtgaatctc tggtgattga aaaataaaca gttttgtacg ttctgcacta
    16741 tgcgaggaag gatttttact aaaaaaaagc cgctggggtt taaaacaccc ccagcggctc
    16801 gttttttaca ctattgtctc aggaattatc tatcgtccgt cgattactcg atatcccttt
    16861 caatcaacaa tgaatcatcc ccttccggag cagacattgg gctgtattgc cacggattat
    16921 ggtagctctc catcgcctga tagatcacct gggccagctc attattactg gacgggtcat
    16981 agcacagcaa atattcggta tcaggtagcg gcggtaagcc atctactcca ctcagcacac
    17041 gcaggtccgg gctcatcatc tcaaccggcc ttgccgtcac gccaagaccg gctttcactg
    17101 ccgcacgaac ggccggaagc gtcgaggcga cataagccag tcgccatgga atatctactt
    17161 tattaagcgt cgccagcacc atatcgcgaa acggacttgg atcatccagc aatacaagag
    17221 ggatcggctc gcctttttgc aatacgtatt ccgctgcgca gtaccagtgt gttggcgagg
    17281 tacgcaggtt caatgcctta aacgctgaag gacgatgggt ggttaccatc aaatccactt
    17341 cctgggattc cagcatttct gccatatagg cattacgttt aacgcggaca tccagcgcta
    17401 atttcggata aaccgaactc acgcgattta acaggaaagg taagatcgta tcggcagatt
    17461 catctgaagc accgatagtt aacacgccct gaagattact gaacattaat gatgagcagg
    17521 cctcatcatt aaaacgcagg attttcctgg cgtaaccaag aagttgaatg ccatgttcag
    17581 ttaacagttt gttgcgaccg tgacgagcga acagttcttt cccaacgagt tgttccagac
    17641 gctgcatttg ctgacttacg gcggactgag tacgacacac agcggcagct gcggcagcaa
    17701 aagtgttcag atcggcaaca gcaacaaatg ttctcagcag atcgaggtcg aggttaatta
    17761 tcggacgatt tgcacttatc atatattatc acttactggc ggctcatact gagctggtta
    17821 atgctgtgca cacacaaaca agcaattcca tttgtaatgt gcctccctgg cagtttcatc
    17881 cggaacccgg acgaaagtaa aaatgcatat gagttgcact aaaaaagcga ctcacattgt
    17941 tccgttataa tgcctgaagt agatcacaga atatatcttc agggatcgca tatctattaa
    18001 gttactcact cttttctatt tatgacatgc gcgtgtttgt ataaatgtaa atgtgagtcc
    18061 ttgttccact ctcgtgcagc atcgctggtc atacgcgaac acgtacaaac agcaatggtg
    18121 aagcggcatc aagagcaggg atccgtcatt tatgcgagca ttttacccca aaaacctttt
    18181 atttataagg gtcattgcga attatctgat gcaaagttat gttatgttag gcaaagtaat
    18241 cttctattat taataagcac atcaaaacct ttttgaat
//
LOCUS       DAZPAB010000070        18203 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZPAB010000070 DAZPAB010000000
VERSION     DAZPAB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18203
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.070"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(62..820)
                     /gene="aroD"
                     /locus_tag="ACHUPG_002100"
     CDS             complement(62..820)
                     /gene="aroD"
                     /locus_tag="ACHUPG_002100"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR0861715.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="ACHUPG_002101"
     CDS             complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="ACHUPG_002101"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR0861716.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1729..3001)
                     /locus_tag="ACHUPG_002102"
                     /pseudo
     CDS             complement(1729..3001)
                     /locus_tag="ACHUPG_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3184..4389)
                     /locus_tag="ACHUPG_002103"
     CDS             complement(3184..4389)
                     /locus_tag="ACHUPG_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0861717.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4489..4845)
                     /locus_tag="ACHUPG_002104"
     CDS             complement(4489..4845)
                     /locus_tag="ACHUPG_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR0861718.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4981..5088)
                     /gene="rprA"
                     /locus_tag="ACHUPG_002105"
     ncRNA           complement(4981..5088)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHUPG_002105"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5274..6386)
                     /gene="ydiK"
                     /locus_tag="ACHUPG_002106"
     CDS             complement(5274..6386)
                     /gene="ydiK"
                     /locus_tag="ACHUPG_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR0861719.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6775..9831
                     /locus_tag="ACHUPG_002107"
     CDS             6775..9831
                     /locus_tag="ACHUPG_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR0861720.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9828..10238
                     /gene="menI"
                     /locus_tag="ACHUPG_002108"
     CDS             9828..10238
                     /gene="menI"
                     /locus_tag="ACHUPG_002108"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR0861721.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10338..10526
                     /locus_tag="ACHUPG_002109"
     CDS             10338..10526
                     /locus_tag="ACHUPG_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR0861722.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11074..11442
                     /gene="sufA"
                     /locus_tag="ACHUPG_002110"
     CDS             11074..11442
                     /gene="sufA"
                     /locus_tag="ACHUPG_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR0861723.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11451..12938
                     /gene="sufB"
                     /locus_tag="ACHUPG_002111"
     CDS             11451..12938
                     /gene="sufB"
                     /locus_tag="ACHUPG_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR0861724.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12948..13694
                     /gene="sufC"
                     /locus_tag="ACHUPG_002112"
     CDS             12948..13694
                     /gene="sufC"
                     /locus_tag="ACHUPG_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR0861725.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13669..14940
                     /gene="sufD"
                     /locus_tag="ACHUPG_002113"
     CDS             13669..14940
                     /gene="sufD"
                     /locus_tag="ACHUPG_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR0861726.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14937..16157
                     /gene="sufS"
                     /locus_tag="ACHUPG_002114"
     CDS             14937..16157
                     /gene="sufS"
                     /locus_tag="ACHUPG_002114"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR0861727.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16170..16586
                     /gene="sufE"
                     /locus_tag="ACHUPG_002115"
     CDS             16170..16586
                     /gene="sufE"
                     /locus_tag="ACHUPG_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR0861728.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16735..17739
                     /gene="ldtE"
                     /locus_tag="ACHUPG_002116"
     CDS             16735..17739
                     /gene="ldtE"
                     /locus_tag="ACHUPG_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR0861729.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17802..18038)
                     /gene="lpp"
                     /locus_tag="ACHUPG_002117"
     CDS             complement(17802..18038)
                     /gene="lpp"
                     /locus_tag="ACHUPG_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR0861730.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4788 a         4399 c         4676 g         4340 t
ORIGIN      
        1 acggttaaaa atactttttt tatttcggcg ggggagggta tttccgccga aatattattg
       61 cttatgcctg atgtaaaata gttaataccg tgcgcaaatc atttaccgag atttgccctg
      121 gcgcagaggc tttttttacc gcaccaaaag ttgccgccga gccaaatact tcaccaacca
      181 gacgagaaat tacgccagtt tttgccatcg acatcgtgat gattggacga tcggcatact
      241 gctcctgcat ctccagggtc gcggcaagca acgtcagcac atcgctggta ctttgcggca
      301 tcagcgcaat cttaggaata tcggcgtcga aggactgcat tttgcgcaga cgggcaatga
      361 tttcttcggc ttccggcgtt ttatggaagt catggttgga catgacaact ttcacatcat
      421 gcgcgtgggc gtaggcgacg gtttctttga cctgatcatc gccggtaaat aactccagat
      481 cgatcatatc aaccaggccg ctgtcgatgg ctgcacgatt gagagcaata taagcctcgg
      541 tggaaatcgc ctgctcgccg ccttctttgg cactgcggaa ggtaaacagc agcggttttt
      601 ctggcatggt ttcacggaga atttttgccg ccgccatgac agactccaca ttggagaggt
      661 cggcaaagtg gtccacacgc cattccagaa tatcaaagtc cgcttcacga taggcgagag
      721 cttcggattt cacgcgggcg atatctttcg ccatcagcga gacgatgatt ttaggtgcgc
      781 ccgcaccaat gacgagatct tttacagtta cggttttcat tttttaccct ttctgcacgc
      841 ggtcagcctg tcaggcaccg aaccccatga cctgtttaac atattccaga gggaaatctt
      901 tgccagtcca taacgtgaac tgttcagccc cttgccacaa caacatgccg tatccatcaa
      961 tcgttttgca accagcttgt tgtgcctgct gcaataactt cgtcatatgc gggttataca
     1021 cgcattcagt gaccagcagt cccggatgta acagactgat atcattaacc aatgattcat
     1081 tctcaagggg tttcatacct acttttgtgc cattggttaa aatgtcggcg gaagccaggg
     1141 cttcagcaaa ggcttgctga tcggcgagat cggtatccgt gacgacacaa tcggtgtttt
     1201 cgttaacccg ctgcgcgaag gcgagggctt tatcgaagaa ctcatcccga cggttaaaga
     1261 gtttaatttc ttttaaacct tcaattgccc cctgcgcgcc aatcgccgtt gaggcacctc
     1321 cggcccctaa cagcaccatc gttttgcctt tgatatcaaa accgctctct ttaatggcgc
     1381 gaatatggcc cgtgccgtcg gtgttatagc cacgcagata gccatcatca ttaacgatgg
     1441 tgttgatggc acccaccagt ttggctgctg gcgttaattc atcaacatat tcacacgcca
     1501 gttgtttgtt cggcatcgac acaccagttc cgcgcatttt gagggctttt aatccttcaa
     1561 ttgctccggg aaagctatcg ttatccactt cgaaggccat ataggtaaat ggcaatcccg
     1621 ctttttctaa ggctttattc tgcatttcag gcgataaact gtggcggata ggataagcca
     1681 tcaatccaat caattcgtat tttgcggtaa catccataat taactccttt aacctctatg
     1741 cttaattgtg tttaaccggg tgctaaattt gcgttcgcca aaccgcacat cattctcagg
     1801 aataataaaa acgcggtaat agcggctaaa aacaataatt gcggtaatca gggccagcag
     1861 cgcgaaagta aaatcgagaa caatgatata ttgcaggccg atgttcgaca gataaccggt
     1921 aatcagcgga ataacaaagt tagccagtcc acccatcatc atataaatac tggtgacttt
     1981 ggctttgctt ttggggaaaa actctgacat gaccgaaaca ccgagctgta aaatgccgcc
     2041 agctgctgag aaaccgataa caaaggcgcc ggcattacac accagtggag aagggtacag
     2101 gtagataatg gcagctgtta ttgttgccag cgcagagtta aatacattag cccagatggg
     2161 ccggaccatt tttttcagta gtgcggcaaa aataaagaca cagaccaacg agcccatact
     2221 gtaataagag atggttttta atgcctcagc ttctgacata ccagcaaaag ccatcgcata
     2281 tttgggcatc cacaccacaa tcacataaaa ggtcgagaat gcggctacac cgaacagtac
     2341 cgatgaaaca ccttccagcc agactaaagg ttttgctgtt catttgcggt aattcattag
     2401 ctacgctggc gtccaccaac tggctgggga atttgctttt caacagcatc agcgtgatca
     2461 gtacaaacag aatacccgga ataatcagcc catagccgta ccagatatta ttgagcaaca
     2521 tatagctcac cagcattggg tagaacattt gcccaaatga caccatcgct ttaaccagta
     2581 tgaccgccga accagaggct ttcggaaagc attccatgag cgcggggtag ccacccgtat
     2641 ccagcgctga gttagcgata cctacgcaca ctgccagacc gtaggcgaga gttaaattcg
     2701 ggcaagcggg aataccaaag aagaatagca gatacattat tactgccatt aatatcaccg
     2761 cccgacgacc aaacttatcg gagatcacac cgaagaataa aatactgatc aatcgcccca
     2821 aaccgatacc ggaaattaaa taggcaatgc ccgcgttgtc agtggaaaac ttttctgaca
     2881 gagatgtcat attttgggca agcgtaataa cactaatgcc gtgcaggaaa tagctgaagt
     2941 aaatacaaag aacagccagg ataaatggcg tgctgaaaga taatttcgtt accgtacgta
     3001 ttgttctccg acggtttaca taattactat actgaagatg attttccctg ggtacatggc
     3061 ataaagttta tgtttccagg aatattgcat gtcatagttt gtttttgatc gcgattttcc
     3121 ttagttagcg cagaaagagt tatttatccg taagatcttg catgacctga aaagaatatt
     3181 gcattaccca cccggagcga cattttcctt tagcgagtga tgccgcgttt ttttgcggct
     3241 gcgtaagccg ataaacagtg ccagtaaaaa accgatggca gtatcgaacc acataatatc
     3301 ggcaatactt ctttgggaca gatgagccgt aatcaacgga atagtaaagg tcgcaatact
     3361 gcccgtactg taatagatcc ctgtagcttt acctttagcg taagggaaac gttcagccat
     3421 taacgtcagg ccaatttgca caacacctcc agcagaggta aaaccaatga caaaagcaaa
     3481 tattatcacc acataaaatg tgggatgcag gcagacggta aacagagcaa taaatgagat
     3541 aaaggtgtac agcatcagta atgttgttgg gcgaacggta ttacgaatga gtggagcggt
     3601 aataaataca caaagcagcg aacccacggt gtagatactg agtagtttga tcgacatagt
     3661 gtatgacatg cctgcaacaa attgtccgta ctgtgccagc cactggctaa ccagataaaa
     3721 cgttgccatt gagatatagc catataaggt ataactggct aaatcgataa ttgaacagcg
     3781 atgttccgta gagctggtgg ttttctttat gacaggtaag cgacgacccg gatggggtgg
     3841 gaacgtacaa cggtataaaa acagagcgtt aataaacata atgcctgcag caatcataaa
     3901 ggaccaaccg aaccacagtt cagcccacac taacaggcta atgattagcg gtaataaaaa
     3961 ttgtccgctg gaaacaaatg ctttaattaa aatattggct gtcccaggtg agcgtggaaa
     4021 agcttccatc aaactgggat aagtgcctgc atcgagaaaa ctgtttgcca ttcccgccag
     4081 aaagccaaaa acataagcga taatgatgtt attggtctgc aggatgtcaa aaaagaaggc
     4141 catatagcag cacatcccga gcatgataaa agggcggcga ccaaagcgat cggataataa
     4201 tcctgcaaaa agcaagacac ttaatcgacc aatgcccagc gatgagataa ctatcgagac
     4261 acccgcggca ttagtctgcc aaagtgtctc cagcgaggcc atattcaggc tcatcaaaat
     4321 gacgcccata ccatgcacca ggtaattaaa atacaaccca agtgcggtag ggtaataggg
     4381 atttttcata ggttctactt agcaaaggtg ctttaaaaag tgtagcgatg ttgtgtcagt
     4441 atccattcat tattgttgta acaacgacaa ttcctttctg tggaaaaatt aacaaagccg
     4501 ctccagatcg tgtgcatata attcagcggc taccgattga tagatcttcc agtcaataaa
     4561 attgccatca gggtagactt gttgaaacgc ggttaagtcg gggaaaaaac gcatggtgtt
     4621 gttgccgatg cggttattta ttttctctat gatggcatga agatgttttt ttcgttgctg
     4681 acgcatagcc aacagttgct cgactacaca atcaggaatg gcgcatttgc cattttccca
     4741 ctgacgccag ctttcactgt caccggtctg ggcaatccag gtcgcgcatt catcaatagt
     4801 catggcaaaa atatggcgga gtgcctggag ttcataagcg ttcatcgtgt aatggctgca
     4861 tgaaataaac aataaaatat aaataacatt atcagcctgc tgacggcttg aagagagtca
     4921 cagtatcttg tgcaacatta ttggaaagag tgaggggcga ggtagcgaag cggaaaaatg
     4981 ttaaaaaaaa gcccatcgtg ggagatggga aaagactaca cacagcaatt cgttgtttca
     5041 ctcaggggat ttccatgctt ataaatcaat atgttgattt ataaccgtga gctaatagta
     5101 ggcatacgga ctttttgcgt cgatcagatt cgtctcatta gtttaaaaac tgtaaagaat
     5161 ttgcgacagg tactaccagc tgaatggcgg taagcaggaa aatatgggga agagcaagca
     5221 aatatcaatt agcggtgatt aacaatcagg cggcagagcc gcctgatgag aaattactta
     5281 ttcggtttct cgatttcgcc aagttcttcg agaatttctt ccggttggtc cgtcggtggc
     5341 gggacttctt ccacccacgc ggcaaacaga cgccaggaaa cggctaacag aaccggacca
     5401 ataaacagac cgatcatccc gaaagcaatc aaaccaccaa taacgccaga gaggatcagg
     5461 atcagcggta aatcggcacc catgcgaatt aacattgggc ggatgacgtt atccagcgtg
     5521 ccaaccacac cgctccacac taacaatacc gttccccagg tggtatcgcc ggtccagtag
     5581 agccagataa tcgccggaat cagtaccggc aacgggccaa gctggacaag gcaggagagg
     5641 atcattaaca ccgttagcaa cgttgcataa ggtacgccgg atacggcgag gccgataccg
     5701 ccaagcactg cctgtactaa cgccgtcacc accacaccca gcgccaccgc gcggatagcc
     5761 tgtgccgcca gcagcacggc ggcatctccg cgaacgcctg ccagacgggt tgcaaaatgg
     5821 cgaatgcctt gtgccacctg ttcaccgcgc cagtacagca gggcactgaa gagaagcatc
     5881 agcgcacaat gcaccataaa gcgcccgata tgcgccgcct gcccaacgaa ccaggtggtg
     5941 gtggtgccaa tataagggcg gactttcgcc atgatcgccg taccccccat atccagcaag
     6001 ttgtgccagc ctgcatacag cttcgcgcca atcaccggaa tggtattaag ccacgccaga
     6061 tcgggtaacg tcatgtcgcc gctggaaatg gctttaatta gcgggccgct gccgtcgacg
     6121 atactgttaa ccagcaaagc aatagggatg ataaacacca tcactaataa cagcgtcatc
     6181 accagaacgg cgagagagcg gcggccaaac atgatctttt gcaaacgtaa caataccggc
     6241 caggtggcga taaccaccgt accggcccat gcaaagccga gaataaaggg ttgaacaatc
     6301 cacagacatg ccacaatcat gatggctaaa aacagcaccg aaagcagaat ttgtgcgaca
     6361 tccctgggct gacgaacatt taccataact acttttcacc ttttttgtgc gtcaaatcgt
     6421 cgacgcgacg tgaacacgcg aaactcacct gcataatgat gagcaatttc agcggttttt
     6481 aacaggccga ttctgccctt aattctgatg ggcgtgttag aaaaaaatgt ggtacaacaa
     6541 ctcaggcaac acgcaaacga tttactcgtc gtatttcaag ttgcatgtgc tgcggctacg
     6601 ttcgttcacc ccagtcactt actattgtaa gctcctggag ttcactcgct tgccgccttc
     6661 ctgcaactcg aaatccatag agtaaataaa catcaccttt cagtgcgcta ttgcagccgc
     6721 ctgaaagaat agtgtatcga cctacatcac agaccgcagg aaagggtcaa tataatgatt
     6781 ccacagattt ccctggcacc cggcgtcgtt caactggtgc ttaatttttt gcaagagctg
     6841 gagcaacaag gttttaccgg cgatgcggcg acaagttatg ccgatcgtct gacaatgtcg
     6901 accgacaaca gtatttacca acttctcccc gatgcggtgg tatttccgcg ttcaaccgca
     6961 gatgtggcgc tgatcgcccg tcttgccgcg caggaacgct attcatcgct gatctttacc
     7021 ccccgcggcg gcggcaccgg cactaacggt caggcgctca accaggggat tattgttgat
     7081 atgtcccgcc atatgaaccg catcatcgaa attaaccctg aagagggctg ggtgcgcgtt
     7141 gaggccgggg tgataaaaga ccaactcaat cagtacctga aaccgttcgg ctactttttt
     7201 gcgccggaac tttcgaccag caaccgggca acgctcggtg ggatgatcaa taccgatgca
     7261 tccggtcagg gatcgctggt ctatggcaaa acgtcagatc acgtacttgg cgtacgcgcg
     7321 gtgttgttgg ggggcgatat tctcgatacg caacctttac ccgtcgagct ggcggaaacg
     7381 ctgggtaaat ccaataccac aatcgggcga atttataaca cggtttatca acgttgccgt
     7441 cagcaacgcc agttaatcat cgacaacttc cccaaactta accgctttct taccggttac
     7501 gatctgcgtc atgtctttaa cgatgagatg accgagttcg acctgacgcg cattctgacg
     7561 ggttcagaag ggacgctggc ctttattacc gaagcgcggc tggatattac gcgcttgcct
     7621 aaagtgcgcc gtctggtgaa tgtcaaatat gactcttttg actctgcgct gcgtaacgcg
     7681 ccgtttatgg ttgaggcgcg ggcgctttcg gtagagacgg tggactcaaa agtgctgaat
     7741 ctggcgcggg aagatattgt ctggcattcc gtcagcgagt tgattaccga tatgcctgac
     7801 aaagagatgc tcggactgaa cattgtggaa tttgctggtg atgatgaggc gctgattgac
     7861 gagcgagtta atgcactctg tgtgcggctt gatgagctga ttgccagcca acaggcaggt
     7921 gtgattggct ggcaggtgtg ccgcgagctg gcgggggttg aacgtatcta tgcgatgcgc
     7981 aaaaaagccg ttggtctgct tggcaatgcc aaaggtgccg ctaagccaat tccgtttgct
     8041 gaggatacct gcgtaccgcc ggaacatctg gccgattata ttgctgaatt tcgcgcgctg
     8101 ctcgacagcc acggcttaag ctacggtatg ttcggtcacg tcgacgcagg tgtcttgcac
     8161 gtccgtccgg cactggatat gtgcgatccg caacaagaga ttttgatgaa gcaaatctct
     8221 gatgacgtgg tggcgctgac tgcgaaatac ggtggtttgt tgtggggcga gcacggcaaa
     8281 ggttttcgcg ctgaatacag cccggcgttt ttcggtgagg aactttttgc agaactgcgc
     8341 aaagtgaaag cggcatttga cccgcataac cgactcaacc cagggaagat ttgcccgcca
     8401 gaaggtctcg atgcgccgat gatgaaagtg gacgcggtga agcgcggtac attcgatcgg
     8461 cagatcccca ttgcggtacg ccagcagtgg cgcggtgcga tggagtgtaa cggcaacggt
     8521 ttatgcttca actttgatgc ccgtagtccg atgtgtccgt cgatgaagat cacccagaac
     8581 cggattcatt caccgaaagg gcgcgcaacg ctggtgcgtg aatggctgcg cttgttggcg
     8641 gatcgcggcg ttgacccact caaactggaa caagaactgc ctgaatccgg cgtcagtttg
     8701 cggacgttaa ttgcccgcac gcgcaatagc tggcatgcga ataaaggcga atatgacttc
     8761 tcacacgaag tcaaagaggc gatgtcgggc tgtctggcct gtaaagcttg ttcgacccag
     8821 tgccccatca aaattgatgt gccggagttt cgctctcgtt ttctgcagct ctatcacacc
     8881 cgttatttac gcccgcttcg cgaccacctc gttgctacgg tcgagagcta cgcaccgctg
     8941 atggcacgcg cgccgaaaac ctttaacttc ttcattaacc agccgctggt gcgcaaactc
     9001 tcggaaaaac atatcggcat ggttgatttg ccactgctgt cggtcccctc gctacaacaa
     9061 caaatggtgg ggcatcgctc ggcaaacatg acgctggaac agcttgaagc tctcaatgca
     9121 gagcagaaag cgcgcacggt gttggtggtg caggacccct ttaccagcta ttacgatgcg
     9181 caagtggtgg cggattttgt ccgtctggtt gaaaaattag gtttccagcc tgtgttactg
     9241 ccattttcgc caaatggcaa agcccagcat atcaaaggct ttcttaatcg ttttgcgaag
     9301 acggcgaaaa agacggcgga tttcctcaac cgtatggcta agcttgggat gccaatggtg
     9361 ggcgtcgatc cggcgctggt actttgttat cgcgatgaat ataaactggc gctgggcgag
     9421 gaacgtggcg agtttaacgt cttacttgcg aatgaatggc tggcaagcgc acttgagtca
     9481 catccagtgg ctacagtcag cggtgaatca tggtatttct ttggtcactg taccgaggtt
     9541 accgctttgc cgggcgcgcc agcacaatgg gccgcgatat ttgcccgttt tggcgcgaaa
     9601 ctggaaaatg tcagcgtggg ttgctgcggc atggcaggga cttacggaca tgaagcgaaa
     9661 aaccatgaaa attcgctcgg gatctatgag ttatcctggc atcaggctat gcagcgactg
     9721 ccgcgtaacc gctgtctggc gaccggatat tcctgccgta gccaggtaaa acgggttgaa
     9781 ggcacggggg tacgccatcc tgtgcaggct ttactggaga ttattaaatg atatggaaac
     9841 ggaaaatcac cctggaagca ctgaatgcta tgggtgaagg aaacatggtg gggttcctgg
     9901 atattcgctt tgaacatatt ggtgatgaca cccttgaagc gacaatgcca gtagactcgc
     9961 ggacaaagca gcctttcggg ttgctgcatg gaggagcatc cgtggtactg gccgaaagta
    10021 tcggttccgt tgccggttat ttatgtaccg aaggtgagca aaaagtggtt ggtctggaaa
    10081 tcaatgctaa tcacgtccgc tcggcacgag aagggcgggt gcgcggcata tgtaaaccgt
    10141 tgcatctcgg ttcgcgtcac caggtctggc agattgaaat cttcgatgag aaagggcgtt
    10201 tgtgctgttc gtcacgattg acgaccgcca ttttgtgacg ttgtcaccag aaaagtgtga
    10261 cgcatattgc ttttggtcat tatctgcaca gtaaacggtg gtatactgtg caggtcttgt
    10321 ctaaaagcga ggatgctatg tctactcaac ttgatcccac ccagctggcc attgaatttt
    10381 tacgccgtga ccagagcaat ctttcacctg cccaatatct caaacgtctg aaacaactag
    10441 aacttgaatt tgctgacctg cttactctct cttctgctga attaaaagaa gaaatctact
    10501 tcgcctggcg tctgggcgtg cattgatcct gacttaatgc cgcaagttcg ccaaatcgac
    10561 ctggattaac ctccgtccag gttcgatgac cttcttaata ctgcttatct accgcctgat
    10621 attgttcaaa aataatacta atcgcagttt gtgttaaaac ggcgggttag ctttatgaga
    10681 ttattcttat cgccccttca agagctaagc cactgagagt gccggagata agcgccggat
    10741 ggggtagaaa cccttaagcc tgtgtcgcac agacttaagg gttttcttat ttctgaacgc
    10801 tgcacttttt tacctatctc ttaaatagtc attttcaata caactttttc tttttcatca
    10861 cagccttaat gctccttaga taataaccat tatctaacaa tgagatacct aattcttagc
    10921 gtgcctgtta acccacacat caggggctat gcttattaaa agcaggcaaa aagaatgttt
    10981 tacggtaaag cccctgcgtt tgctgggttg aactgataat cattatcact aacatgctgt
    11041 tatacgctga aagcgatgaa gtgaggtaaa tagatggaca tgcattcagg aacctttaac
    11101 ccacaggatt tcgcctggca aggcttaacg ctgacacccg cagcggcgat acacatccgc
    11161 gagctggtgg caaagcagcc aggtatggtc ggcgtgcgct taggtgtgaa gcaaacgggc
    11221 tgcgcgggct ttggctatgt gctcgacagt gttagcgagc cggacaaaga cgatctgctg
    11281 tttgaacacg acggcgcgaa gctgtttgtc ccgctgcaat cgatgccgtt tattgatggc
    11341 acggaagtcg atttcgttcg tgaaggactt aatcagatat tcaaatttca caaccctaaa
    11401 gcccagaatg aatgtggctg tggcgaaagc tttagggtat aggcggtact atgtctcgta
    11461 atactgaagc aactgacgat gtcaaaacct ggaccggcgg cccgctgaac tataaagaag
    11521 gattcttcac ccagttagcc accgatgagc tggcaaaggg gataaacgaa gaggtggtgc
    11581 gcgcaatttc ggcgaagcgt aatgagccgg agtggatgct ggagtttcgt ctaaacgcct
    11641 atcgcgcatg gctggagatg gaagaaccgc actggttgaa agcgcactac gacaagctga
    11701 attatcagga ttacagctac tactcagcac catcgtgcgg taattgtgac gacacttgcg
    11761 cgtctgaacc tggcgcggtg cagcaaactg gcgcgaacgc ctttttaagt aaagaggtgg
    11821 aggcggcgtt tgagcagttg ggcgttcccg tgcgggaagg caaagaggtg gcggtggatg
    11881 ccattttcga ctctgtttcg gttgccacca cttatcgcga aaaactggcg gagcagggaa
    11941 ttattttctg ttcctttggt gaggcgatcc acgatcaccc ggaactggtg cgtaaatatc
    12001 tcggcaccgt ggtgccgggg aatgacaact tctttgcagc acttaatgcg gcggtagcct
    12061 ctgacggtac gtttatttat gtgcctaaag gcgtgcgctg cccgatggaa ctttccacct
    12121 attttcgcat taacgcggaa aaaaccgggc agtttgagcg cacaattctg gtggccgacg
    12181 aagacagcta cgtcagctac attgaaggct gttccgctcc ggtgcgtgac agctatcagt
    12241 tgcacgcggc agtggtggaa gtcatcatcc ataaaaacgc cgaggtgaaa tattccacgg
    12301 tacaaaactg gttccctggc gataacaaca ccggcggtat tctcaacttc gtcaccaagc
    12361 gtgctttgtg cgaaggcgaa aacagcaaaa tgtcatggac gcaatcagaa accgggtcag
    12421 cgattacgtg gaaatatccc agctgcattt tgcgcggcga taactccatt ggtgagtttt
    12481 actcagtggc gctgaccagc ggtcatcagc aagcggatac cggcaccaag atgatccaca
    12541 tcggtaaaaa caccaaatcg accattatct cgaaagggat ctctgccgga catagtcaga
    12601 acagttatcg cggcttagtg aaaatcatgc cgacggcaac caatgcgcgc aatttcactc
    12661 agtgcgactc aatgctgatt ggcgctaatt gtggggcgca taccttcccg tatgtcgaat
    12721 gtcgcaataa cagcgcacaa ctggagcacg aggcaacgac atcacgtatt ggtgaagatc
    12781 aactgtttta ctgcctgcaa cgcgggatca gcgaagaaga cgccatctcg atgattgtta
    12841 acggtttctg caaagacgtg ttctcagaac tgccgctgga atttgccgtt gaagcacaaa
    12901 aactcctcgc catcagtctt gaacacagcg tcggataagg aataaacatg ttaagtatta
    12961 aagatttaca cgtcagcgtg gaagataaag ctatcctgcg tggattaagc ctcgacgttc
    13021 gtcccggcga agttcacgcc attatggggc caaacggttc gggcaaaagt accttatcgg
    13081 caacgcttgc cgggcgcgaa gattatgaag tgacgggcgg cacggttgag ttcaaaggca
    13141 aagatttgct tgcactgtcg ccggaagatc gtgcaggcga aggcatcttt atggccttcc
    13201 agtatccggt ggagattcca ggcgtcagta accagttttt cctgcaaacg gcacttaatg
    13261 cagtgcgcag ctatcgcggc caggaaacgc tcgaccgctt tgattttcag gatttgatgg
    13321 aagagaaaat cgctctcctg aagatgccgg aagatttatt aacccgttcg gtaaacgttg
    13381 gtttttccgg cggcgagaaa aagcgcaacg atattttgca aatggcggtg ctggaaccgg
    13441 agttatgcat tcttgatgag tcggactccg ggctggatat tgacgcatta aaagtggtcg
    13501 ccgatggcgt gaactcgctg cgtgatggca agcgctcatt catcattgtt acgcactacc
    13561 aacgcattct cgactacatc aagcctgatt acgttcatgt gctatatcag ggacgaattg
    13621 tgaaatccgg cgatttcacg ttggtcaaac aactggagga gcagggttat ggctggctta
    13681 ccgaacagca gtaatgcgct gcaacagtgg catcacttgt ttgaagctga agggacaaaa
    13741 cgctcaccgc aagcacagca gcatttacaa caattgctgc gtaccggact gccgacacgt
    13801 aaacatgaaa actggaaata tacgccgctg gaagggctga ccaatagtca gtttgtcagc
    13861 attgcgggag atatatcccc acagcagcgt gatgccttag cgttaacgtt agacgccgtg
    13921 cggctggtgt ttgtcgatgg acgttacgtg cccgcgctga gcgatgcaat tgaaggcagc
    13981 ggatatgaag tgagcattaa cgacgaccgt caggggttac ccgacgctat tcaggcggaa
    14041 gtgtttctgc atttgacgga aagcctggca caaagcgtga cgcatatcgc cgtgaagcgc
    14101 ggtcaacggc cggcaaagcc attgctgtta atgcatatca cccagggcgt ggcaggtgaa
    14161 gaggtgaaca ctgcccatta ccggcatcat ctggatctgg cggaaggtgc cgaagcaacg
    14221 gtgatcgaac attttgtcag ccttaatgat gctcgtcatt ttaccggggc acggttcact
    14281 atcaatgtcg cagcgaacgc ccacttgcag catatcaagc tggcgtttga aaacccggtc
    14341 agtcaccact ttgcccataa cgatttgttg ctggctgacg atgccaccgc atttagccat
    14401 agtttcctgc tgggtggcgc agtgttacga cacaacacca gtacgcaact caatggcgaa
    14461 aacagcacgc tgcggatcaa tagcctggcg atgccggtga aaaacgaggt gtgtgatacc
    14521 cgtacctggc tggaacacaa taaaggtttt tgtaacagcc gacagttgca caaaactatc
    14581 gtcagcgaca aaggccgcgc ggtatttaac ggtttgatca acgtcgcgca gcacgccatc
    14641 aaaacggatg gtcagatgac caacaataat ctgctgatgg gcaaactggc ggaagtggat
    14701 acaaaaccgc agctggaaat ctatgcagat gatgtgaaat gcagccacgg cgcgacggtg
    14761 gggcgtattg atgatgaaca gatgttctat ctgcgctcgc gcgggatcaa tcagcaggat
    14821 gcccagcaga tgatcattta cgcctttgct gccgaactga cggaagcact gcgtgatgag
    14881 gggcttaaac agcaggtgct ggcccgaatc ggtcaacggc tgccaggagg tgcaagatga
    14941 ctttttccgt cgacaaagtg cgggccgact ttccggtgct ttcgcgtgag gtaaacggtt
    15001 tgccgctggc ttatctcgac agcgccgcca gtgcacagaa accgagccag gtgattgacg
    15061 ccgaggccga gttttatcgt catggctacg cggcggtgca tcgcggtatt cataccttaa
    15121 gcgcccaggc gaccgagaaa atggagaacg tgcgcaagcg ggcatcgctg tttattaatg
    15181 cccgttcggc ggaagagctg gtgtttgtcc gcggcacgac ggaagggatc aatctggtcg
    15241 ccaatagctg gggcaacagc aatgtgcggg cgggcgataa catcatcatc agtcagatgg
    15301 agcaccacgc taacattgtc ccctggcaga tgctttgcgc acgcgttggc gcagagctgc
    15361 gtgtgatccc tctcaacccc gacggtacgc tgcaactgga gacgctgcct acgctgtttg
    15421 atgagaaaac tcgcctgctg gcaattactc atgtctccaa cgtgcttggc acagaaaatc
    15481 cactggcgga aatgatcacg cttgcgcacc agcatggcgc aaaagtgctg gtggatggcg
    15541 ctcaggcggt gatgcatcat ccggtggatg ttcaggcgct ggattgcgac ttttacgtgt
    15601 tctccgggca taaactgtat ggccccaccg gaattggcat tctttatgtg aaagaagcct
    15661 tgttgcagga gatgccgccg tgggaagggg gcggttctat gatcgccacc gtcagcctga
    15721 gtgaaggcac tacctggacc aaagcaccat ggcggtttga agccggtaca cccaataccg
    15781 ggggcatcat tggtcttggc gcggcgctgg agtatgtttc ggcgctgggg cttaatagca
    15841 tagccgagta tgaacagaat ctgatgcatt atgcgctatc acagctggaa tctgtaccgg
    15901 atctcactct ctatggccaa caaaacaggc ttggcgttat tgcttttaat ctcggtaaac
    15961 accacgccta tgatgttggc agttttctcg ataattacgg cattgctgtg cgtaccggac
    16021 atcactgcgc tatgccatta atggcctatt acaacgtccc tgcgatgtgt cgggcgtcgc
    16081 tggccatgta taacacccat gaagaagtgg agcgtctggt gaccggcctg caacgtattc
    16141 accgtctgct gggataacag ggaggcacta tggctttatt gccggataaa gaaaaattgc
    16201 tgcgtaattt tttacgctgc gccaactggg aagagaaata tctctacatt attgagctgg
    16261 gccagcgtct gccagaatta cgcgccgaag acggaagccc acaaaatagc attcagggct
    16321 gccagagtca ggtgtggatt gtcatgcgcc agaatgcgca gggaattatt gaattgcagg
    16381 gcgacagcga tgcggcgatt gtgaaagggc ttattgcggt cgtctttatt ctctacgatc
    16441 agatgacgcc gcaggatatt gtcaatttcg atgtgcgtcc gtggtttgaa aaaatggcgc
    16501 tcacccaaca tctcacccca tctcgttcac aaggtctgga agcgatgatt cgcgcaattc
    16561 gcgccaaagc cgctgcactt agctaaacta aagggacagc tttcatccgg ttggctcaca
    16621 ttgccggatg gtcaatgttt acactgaccg gcacaggagt gttttggttt caggtgaaca
    16681 taagcaccat ccgacgtttc agcgtgagtc tccggcaaat acaggaggtt tacaatgaaa
    16741 cgcgcgtctt tgcttacact cacgcttatc ggcgctttta gcgccatcca ggctgcctgg
    16801 gcggttgatt atccgctacc accaaccgga agccgactgg ttgggcaaaa tcaaacgtat
    16861 acggtgcaag aaggggataa aaaccttcag gctattgccc gacgttttga tactgcagca
    16921 atgttgatcc ttgaagccaa taacactatc gccccggtgc caaaacctgg tacgacgata
    16981 actattcctt cacaactgtt attacctgat gcaccgcgtc aggggattat cgttaacctt
    17041 gcagagctgc gcctttatta ttatccgccg ggagaaaata ttgtgcaggt ttatccaata
    17101 ggtattggat tgcaggggct ggaaacgccg gtgatggaaa cgcgtgttgg gcagaaaatc
    17161 cctaacccaa cctggacgcc tacggcaggc attcgtcagc gttcgctgga gcgtggcatt
    17221 aaattaccgc cagtcgttcc tgccggacca aataacccgc taggacgtta cgcactgcgc
    17281 ctcgcgcatg gtaatggcga atacctcatt catggcacca gtgcgccgga cagcgttggt
    17341 ttgcgcgtca gttcagggtg tattcgcatg aatgcaccgg atattaaagc cttgttctcc
    17401 agcgtgcgga cgggaacgcc ggtgaaagtg atcaacgaac cggtgaaata ttccgtggaa
    17461 cctaacggga tgcgttatgt tgaagtacat cgaccactat cagcagaaga acagcaaaac
    17521 gttcagacaa tgccatacac actgccagca ggctttacgc aatttaaaga caataaggct
    17581 gtagatcaga agttagtcga taaagcgttg tatcgtcggg cagggtatcc ggtttcggtg
    17641 agcagtggag caactcccgc agccagcaat gcgccttcag tagagtcagc gcagaatggt
    17701 gaaccagagc aagggaatat gttacgcgcg acgcagtagt ggtaaatagc agacaaaaaa
    17761 atggcgcaca atgtgcgcca tttttcactt cacaggtact attacttgcg gtatttagta
    17821 gccatgttgt ccagacgctg gttagcacga gctgcgtcat ctttagcagc ctgaacgtcg
    17881 gaacgcattg cgttcacgtc gttgctcagc tggtcaactt tagcgttcag agtctgaacg
    17941 tcagaagaca gctgatcgat tttagcgttg ctggagcaac ctgccagcag agtagaaccc
    18001 aggattaccg cgcccagtac cagtttagta gctttcatta ttaataccct ctagattgag
    18061 ttaatctcca tgtagcgtta caagtattac acaaagtttt ttatgttgag aatatttttt
    18121 tgatgggaag gcacttattt ttgatcgttc gctcaaagaa gcatcgaacg ctgggtttta
    18181 gataaaaaaa ttgagagaaa aca
//
LOCUS       DAZPAB010000071        18140 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZPAB010000071 DAZPAB010000000
VERSION     DAZPAB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.071"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            201..1322
                     /gene="roxA"
                     /locus_tag="ACHUPG_002118"
                     /gene_synonym="ycfD"
     CDS             201..1322
                     /gene="roxA"
                     /locus_tag="ACHUPG_002118"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR0861731.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(1371..2597)
                     /gene="pepT"
                     /locus_tag="ACHUPG_002119"
     CDS             complement(1371..2597)
                     /gene="pepT"
                     /locus_tag="ACHUPG_002119"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR0861732.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            2847..3983
                     /gene="potA"
                     /locus_tag="ACHUPG_002120"
     CDS             2847..3983
                     /gene="potA"
                     /locus_tag="ACHUPG_002120"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR0861733.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            3967..4824
                     /gene="potB"
                     /locus_tag="ACHUPG_002121"
     CDS             3967..4824
                     /gene="potB"
                     /locus_tag="ACHUPG_002121"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR0861734.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            4821..5615
                     /gene="potC"
                     /locus_tag="ACHUPG_002122"
     CDS             4821..5615
                     /gene="potC"
                     /locus_tag="ACHUPG_002122"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR0861735.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            5612..6658
                     /gene="potD"
                     /locus_tag="ACHUPG_002123"
     CDS             5612..6658
                     /gene="potD"
                     /locus_tag="ACHUPG_002123"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR0861736.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(6814..7635)
                     /gene="cobB"
                     /locus_tag="ACHUPG_002124"
     CDS             complement(6814..7635)
                     /gene="cobB"
                     /locus_tag="ACHUPG_002124"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR0861737.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(7651..8562)
                     /gene="nagK"
                     /locus_tag="ACHUPG_002125"
     CDS             complement(7651..8562)
                     /gene="nagK"
                     /locus_tag="ACHUPG_002125"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR0861738.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(8591..9835)
                     /gene="lolE"
                     /locus_tag="ACHUPG_002126"
     CDS             complement(8591..9835)
                     /gene="lolE"
                     /locus_tag="ACHUPG_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR0861739.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(9835..10536)
                     /gene="lolD"
                     /locus_tag="ACHUPG_002127"
     CDS             complement(9835..10536)
                     /gene="lolD"
                     /locus_tag="ACHUPG_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR0861740.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(10529..11728)
                     /gene="lolC"
                     /locus_tag="ACHUPG_002128"
     CDS             complement(10529..11728)
                     /gene="lolC"
                     /locus_tag="ACHUPG_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR0861741.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            11990..13063
                     /locus_tag="ACHUPG_002129"
     CDS             11990..13063
                     /locus_tag="ACHUPG_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR0861742.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            13191..16637
                     /gene="mfd"
                     /locus_tag="ACHUPG_002130"
     CDS             13191..16637
                     /gene="mfd"
                     /locus_tag="ACHUPG_002130"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR0861743.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            16780..17742
                     /gene="ldtC"
                     /locus_tag="ACHUPG_002131"
     CDS             16780..17742
                     /gene="ldtC"
                     /locus_tag="ACHUPG_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFR0861744.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(17825..18037)
                     /locus_tag="ACHUPG_002132"
     CDS             complement(17825..18037)
                     /locus_tag="ACHUPG_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HFR0861745.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         4408 a         4773 c         4581 g         4378 t
ORIGIN      
        1 tcgttgcaca aagggctaca acgtgaaaca agctggaaca aggaaaatca caaatctgtt
       61 tataatcaaa aacataatga acaatctgaa acattatgaa acggcaaaaa acactaattg
      121 ataaaatatg tccatacttc acgcattacg ttaagcatcc gttataatcg gttgcagata
      181 ccagcctgtg gatgcttaac atggaatacc aactcactct taactggccc gattttcttg
      241 aacgtcactg gcagaaacgc ccggtggtgt taaaacgcgg ctttaataat tttattgacc
      301 cgatctctcc agacgagttg gcgggtctgg cgatggaaag cgaagttgac agtcgactgg
      361 tcagtcacca ggatggcaaa tggcaggtca gccacggccc gttcgaaagc tacgatcatc
      421 tcggtgaaac caactggtca ttactggtac aggcagtaaa ccactggcat gagccgaccg
      481 ccgcgctgat gcgaccgttc cgtgaactac cggactggcg tattgatgat ctgatgattt
      541 ctttttctgt acctggcggc ggcgtcggcc cgcatctcga tcagtacgac gtgtttatca
      601 ttcagggtac cggacgtcgt cgctggcgag tgggcgaaaa gctgcaaatg aaacagcact
      661 gcccacatcc ggatctgtta caggtcgatc cgttcgaagc catcatcgat gaagagctgg
      721 agcctggtga tattctttat attccgccag gattcccgca tgaaggctac gcgctggaaa
      781 atgcgatgaa ctattccgtg ggttttcgcg cgccaaatac gcgggaatta attagcggat
      841 ttgccgatta tgtgctgcaa cgtgaactgg gcggcaacta ctacagcgat ccggatgttc
      901 cacctcgtgc tcatcctgcg gatgttctgc cgcaagagat ggataaactg cgtgagatga
      961 tgctcgaatt gatcaaccag ccggaacact ttaagcaatg gtttggcgag tttatatccc
     1021 agtcacgtca tgaactggat atcgcgccgc cggaaccgcc ttatcagcca gatgaaatct
     1081 acgatgcgct gaaacaaggt gatgtgctgg tgcgcctggg tggtctgcgc gtattgcgca
     1141 ttggcgacga cgtgtatgcc aatggtgaga agatcgattc cccgcaccgt ccggcactgg
     1201 atgcactcgc cagcaacatt gcgctgactg cggagaattt tggcgatgcg ctggaagatc
     1261 cgtcattcct cgcgatgctc gcggcgctgg tcaatagcgg gtattggttc ttcgaagggt
     1321 aagtttggat ttaagccgga tgcagcatca catgccgcat cccttttcgc ttacttccgt
     1381 tgcgccgtta actcggcaat acggacgatc acctgcaccg ctttttccat accttccaga
     1441 atcacaaact catgcttacc atgatagttg taaccgccag tgaacaggtt cgggcaaggt
     1501 aatcccataa acgacaactg cgcgccgtcg gtaccgccgc ggatcggttt cagttccggt
     1561 tcaatatcgc agtcacgcat cgcctgctgg gcgatatcga gaatatgcgg atgctcaacc
     1621 actttctcgc gcatattgta gtaactgtct tcaatcacca attcaatgta gcaatcagga
     1681 tgtaaccctt tgcccacttt tttggcgatc tccatcattt tacgtttacg cgcttcaaac
     1741 tgtttacggt cgaaatcacg gatgatgtag tgcatatcgg cccgttcaac ggtgcctttc
     1801 atgctcgcca ggtgatagaa accttcatag ccttctgtca tttccgggct ttcatccgcc
     1861 ggaacttccg catgaatacg tgccgccagc gacagcgcat ttaccatcac tcctttcgcc
     1921 gtgcccggat gaacattgtt accgacaatt tttatattga ccgatgcggc gttgaagttt
     1981 tcaaactcca gttcgcctac gccaccaccg tcaacagtgt aagcccagcg ggcatcgaag
     2041 gcatcaacat caaaatgttt cgcccctttg cccacttctt catccggggt aaaggcgacg
     2101 cgaatatcac catgcggaat gtttttctgt tgcaataccg ccagcgcggt catgatttct
     2161 gcaatacctg ctttgtcatc agcacctaac aaggttttac catcggtggt aatcaacgtc
     2221 tgacccagta gctgatgcag caccgggaac ataaccggtg ataaaacttc atcgccgata
     2281 cccagcgcaa tatcgccacc gcgatagttt tcaacaattt gcggattaac atttttgccg
     2341 ctgcaatccg gtgaggtatc cacatgagaa ataaagccaa tcgccgggat atcgccaggg
     2401 acgttagccg gtaacgtcgc catcaaagtg cccttctcac ttaaggtcac attgataagc
     2461 cccatttctt cgagctgctc tttcagcaga tgcaataact tccattggcc ttccgtgctg
     2521 ggaacctgtc tcacccctgc ttttgattgg gtatccagag acacgtagtt caaaaatcgc
     2581 tcaagtagtt tatccatgta gtcaccctca ctttttgtga caacattatt aagaagcaag
     2641 aaaagacaaa tattgcgtca ggtcactttt acccctgcaa gcgggaatat ttatcagcat
     2701 taccttaatg aatataaagc taagccagta attcacaaca aagattggcg attcaagcgg
     2761 tttgccgtag aattaccgcc ctcattagga gtcaccaagg tggttaacca caaaccccgc
     2821 atcggtaagc catccgttgc gtttacatgg gacagagtaa aaaattgaat aaacaaccga
     2881 gttcgctttc accgctggtg caattggcgg gaattcgcaa atgctttgat ggtaaagagg
     2941 tcattcccca gctggatctg actatcaaca atggcgagtt cctcacgctg cttggccctt
     3001 ctggctgcgg taaaacaacc gttcttcgcc tgattgcagg tctggaaact gttgattccg
     3061 gacgcatcat gctggataac gaggacatca cccacgttcc ggcggaaaac cgctatgtga
     3121 acactgtttt ccaaagctat gcacttttcc cccacatgac cgtgttcgaa aatgtggcct
     3181 ttgggttgcg catgcaaaaa acccccgctg ctgaaattac gccccgcgtg atggaagccc
     3241 tgcggatggt gcagctggaa accttcgctc aacgtaaacc gcatcagctc tctggtggtc
     3301 aacagcaacg cgtcgccatt gctcgagcgg tggttaacaa gcctcgtctg ttgttgctgg
     3361 atgagtcgct ctcagcgctg gattacaaac tgcgtaagca aatgcagaac gagctgaaag
     3421 cgttacagcg taagcttggc attacattcg ttttcgtgac tcacgaccag gaagaagcac
     3481 tcaccatgtc ggacaggatt gtggtgatgc gcgatggtcg cattgagcaa gacggcacgc
     3541 cgcgtgaaat ctacgaagag ccgaaaaacc tgtttgttgc cgggttcatt ggcgaaatta
     3601 atatgtttaa cgccacggtg atcgagcgac tcgacgagca gcgcgtacgc gccaacgttg
     3661 aaggccgcga atgtaatatc tacgttaact tcgccgttga accggggcaa aaactgcatg
     3721 ttctgctgcg cccggaagac ttacgtgttg aagagattaa cgacgacaac cacgctgaag
     3781 ggctgattgg ttacgttcgt gagcgtaact acaaaggcat gacgctggag tcggttgttg
     3841 aactggaaaa tggcaagatg gtgatggtca gcgaattctt caatgaagac gatcctgact
     3901 ttgaccactc tctcgaccaa aaaatggcca ttaattgggt agaaagctgg gaggtcgtac
     3961 tggctgatga agaacacaag taagttccag aatgtagtga ttgtcactat tgtcggttgg
     4021 cttgtgttgt ttgtctttct gcccaacctg atgatcattg gcaccagctt tttgacccgc
     4081 gacgacgcca gtttcgtcaa aatggtcttt acactggata actacacgcg tctgctcgat
     4141 ccgctctatt ttgaagtgct attgcactcg ctgaatatgg cgctgatcgc caccctcgcc
     4201 tgcctggtgc tgggctatcc atttgcctgg tttctggcga agctgccaca caaggtgcgt
     4261 ccgctgctgc tgtttctgct gattgttccg ttctggacca actcattgat tcgtatctac
     4321 gggctgaaaa ttttcctcag caccaaaggc tatctcaacg agtttttgct ctggctgggc
     4381 gttatcgaca caccaatccg tatcatgttc acgcccagtg cggtgattat cggtctggtt
     4441 tacattctgc tgccgtttat ggtgatgccg ctgtactcca gtatcgaaaa actggataag
     4501 ccgttgcttg aggcggcgcg cgatctcggt gccagcaagt tacagacttt tatccgcatc
     4561 attattccac tgacgatgcc ggggattatt gccggatgtc tgctggtgat gctgccagcg
     4621 atgggcctgt tctatgtgtc cgacctgatg ggcggtgcga aaaacctgct gatcggtaac
     4681 gtcatcaagg tacagttcct caatattcgt gactggccgt ttggtgcagc caccagcatt
     4741 acgctgacta tcgtaatggg cctgatgttg ctggtttact ggcgcgcttc tcgtttgctg
     4801 aataagaagg tggaactcga atgatcggtc gactgcttcg cggcggtttt atgaccgcta
     4861 tctacgcgta cctgtatatc ccaatcatta ttttgattgt gaactccttt aacagctcgc
     4921 gctttggcat caactggcag ggttttacca ccaaatggta tagcctgctg atgaacaacg
     4981 acagcctgtt acaggcagcg cagcattcac taacaatggc ggtgttttcg gcgacgtttg
     5041 ctacgcttat cggttcactg acggcagttg cgctgtaccg ttatcgcttt cgcggtaagc
     5101 cgttcgttag cggaatgctg tttgtggtaa tgatgtcacc agatatcgtg atggcaattt
     5161 cgctgctggt gctgtttatg ctgctgggta ttcagcttgg cttctggtcg ctgctgttct
     5221 cgcatatcac cttctgcctg ccatttgtgg tggtgacggt gtattcgcgc ctgaaaggtt
     5281 ttgacgtgcg gatgctggaa gcggcgaaag atctcggtgc cagcgaattt accattctgc
     5341 gaaaaatcat tctgccactg gcaatgccag cggtggcggc gggctgggtg ttaagcttta
     5401 ccctgtcgat ggacgatgtg gtagtttctt cattcgtcac cggaccaagt tatgaaattc
     5461 tgccgttaaa aatttattcg atggtcaaag tcggcgtatc gccggaagtt aacgcgctgg
     5521 caaccatatt actggtgctg tcgctggtga tggttattgc cagccagctt attgctcgtg
     5581 ataaaacgaa aggtaacaca ggggacgtta aatgaaaaaa tggtcacgcc acctgctcgc
     5641 ggcgggtgct ctggcactgg gcatgagcgc cgctcacgcc gatgacaaca acacgctgta
     5701 tttctacaac tggaccgagt acgtgccgcc aggactgctt gaacagttca ccaaagaaac
     5761 cggtattaag gttatctatt cgacttacga gtcgaacgaa accatgtacg ccaagctgaa
     5821 aacgtacaaa gacggtgcct atgacctggt ggttccttca acctattacg tcgataaaat
     5881 gcgtaaagag gggatgatcc agaagatcga caagtcgaag ttaacgaact tcagcaatct
     5941 cgatccagac atgctcaaca agccttttga cccgaataac gactactcca ttccgtatat
     6001 ctggggtgcg acggcgattg gtgttaacgg tgatgcggtg gatccgaaat ctgtcaccag
     6061 ctgggccgat ctgtggaagc cagagtacaa aggcagcctg ctgttgaccg acgatgcccg
     6121 tgaagtgttc cagatggcgc tgcgtaagct gggctactcc ggtaacacca ccgatccgaa
     6181 agagattgaa gctgcatata acgagctgaa aaaactgatg ccaaacgtcg cagcgtttaa
     6241 ctccgataac ccggctaacc cgtacatgga aggcgaagtt aacctcggca tgatctggaa
     6301 cggttctgct ttcgttgcac gccaggcggg tacgcctatt gacgtggtgt ggccgaaaga
     6361 aggcggcata ttctggatgg acagcctggc gatcccggca aatgccaaaa acaaagaagg
     6421 cgcgctgaaa ttgatcaact tcctgctgcg cccggatgtg gcaaaacagg ttgctgaaac
     6481 tatcggttat ccaacgccaa accttgcggc gcgtaagctg ttaagtccag aagtggcaaa
     6541 cgataagacg ctctacccgg atgctgaaac cattaaaaat ggcgaatggc agaatgacgt
     6601 tggcgcagcc agcagcattt atgaagagta ttatcagaag ctgaaagcag gacgttaatt
     6661 cagcaagcgg gcggtggtat taccgcccgt tttttagtta gcaaggatgt ggctaatgag
     6721 tcaggacagt aaagttttat cccgctcttt cgtcggtatt gtggtccgct tgtaggcctg
     6781 ataagcgtag cgcatcaggc aatgcttcct gctttataat ccctccagca acttttcaac
     6841 aaattctggc accacctggc ttgccgggcc gtaatatttc tcggcaaatt cattaccaac
     6901 ctgactcggt tcaagattca gttcaacggt gtgagcacca tgtagtttgg cttcgtgtac
     6961 aaacccagcc gccggataga catgcccgga agtaccaatg gcaataaaga catccgccat
     7021 cgacaacgcc atataaattt catccatgcc gagtggcatt tcgccaaacc aaacgacgtg
     7081 cgggcgcaag ggtgccggga actggcaaca atggcattta tcttctggcg taacgtctcc
     7141 ggtccagtcg agaacctgac cactttgcga acaacgaact ttcagcaatt ccccatgcat
     7201 atgaatgaca ttggtattac ctgcgcgttc atgcaggttg tctatattct gcgttaccag
     7261 caaaaagcga tcgccgagag cgtcttgcag tttagccagc gcaagatgcg cggcgttagg
     7321 ctgaatttct ggctgctgca gctgtcgacg acgggcgtta taaaacgctt gcaccagttc
     7381 aggatcgcga tcgaaacctt ccggcgttgc cacatcttca acccgatgtt cttcccacag
     7441 gccatctgcg gcgcgaaagg tacgaatacc tgattccgca gaaattcctg cccctgtcag
     7501 tacgagtact cttggttttt ccattgcttc cggcaccact ttatctctga aaaaaatacg
     7561 ctgacgcaaa cgctcgcgca ggcgacgttt atttttacga aaacgactta accgatgacc
     7621 ccgacgcgac agcatagcaa cctctgttgt ttaatcggtt agatgtagga aggccgcacc
     7681 acgcattcct cccgcatcac cgtggcgcgc gcgttcaatg cgtggaacac gagctacagg
     7741 taagagatga caaggcaacc tgtccgccag ttgcgttgtg attgccggga aattcgataa
     7801 gccaccacca atgacgacca ggtcagggtc aacaatggtc aggatatttc ccagagaaac
     7861 cgctaataaa tccagataac gctcaacgtg cgcccttgcc tgctcatcgc cttgatcata
     7921 aagcgcaata atttcgggag cctgcaacgg ttgatgataa tagtgttgat acagccacgc
     7981 aaaaccgcga ccagacagat aattttcaat gcagccatgc tgaccacagc cgcagcggcg
     8041 taacgggaaa tccaacccca tcatggttaa cgcatcaacc ggcagacgca tatggccaaa
     8101 ctcgccggta atgtagcttt taccagtaat cggtttgccg ttgaaaacca gcccgccgcc
     8161 aacgccggtg ccgagaatta accccatcac cagtggatat tgcgtaaatt catcatccca
     8221 ggcttctgaa agggcaaaac agttggcatc gttatcaagg cgtacatcgc gatcaagacg
     8281 tgcgctcagg tcggcacgca gcggtttgcc gctggcagcg gggacattag cggcatacag
     8341 cttaccatct tctgtttccg gcattcccgg aataccgatg ccgacagaac ctttacagcc
     8401 aaaacgttgg tcagcttcag ctaccagctc acacactgca tctaaaaatg cgtcatagct
     8461 gtcacgcggt gtcggcaccc gcttttccca ctgcaactgc cgaccgctat caaacacgcc
     8521 aagcgcaatt tttgttccac caatatcaaa cccgtaatac attgccgctc cttgttttaa
     8581 tgtactgcct ttactggccg ctaaggactc gcgcagggtc aatattactg gcgcgccgcg
     8641 ccggatacca acttgccaaa agactcagca acaatgctgt gaccagtacg tagaagacgt
     8701 ccagccagtg caattccgat ggcaggaagt caataaaata aatatcgctg gagaggaact
     8761 gatgaccgat cagcttttca atccactcaa taatcggggt aagttgcagt gaaacaacta
     8821 cgccgataac caccccacac aggctgccga acagccctgc cagcaatcca taccagacaa
     8881 agatggcgcg aattaaacca tctttcgccc ccagcgttct taatactgcg atatcgccac
     8941 tcttgtcttt caccgccatc actaaggtgg agacgatgtt gaaacaggcc acgccaatca
     9001 ccagtaccat cgccagatac ataatggcgc ggatcatctg gatatcgcga tacatatagc
     9061 cgtaagtacc aatccaactt ttaatataaa catagctgtt ggtcacttca cccgcatcgc
     9121 gtaccagctt attggcgttg aaaacatccg tcattttaag ggcaatacct gacacgctgg
     9181 aacccatatc aagatattgt tgggcatccg ccagcgggat catggcaaaa ctgtgatcga
     9241 gttgaccact caactgcaaa ataccggcaa tgtgcaaacg cacacgtttt ggctgcatca
     9301 gtttatgctc gggattcgag ttggggatca tgatcgacac ccaatcgccc tgcttcactt
     9361 tcagcgcatc cgccacgcct ttgccgatga taatttgctg ttcgcccgct ttaaaattgc
     9421 gccacgcatc cccctgaaca aacgagggta atgcgctcag acgctgttcc tgttgtgggt
     9481 taacgccctt cacctggatt gcgcgcagat tcgctccact ttccaccaga ccggtgaaat
     9541 tgatatacgg cgcagcggcg gcaatacccg gcactttctg cacgttatcc agtgcttcct
     9601 gccagttagt ccatggctga tttaccgctt cgatttcgcc atgcggcacc accgccagaa
     9661 tgcggttatt cagttcgcgt tcaaagccgt tcatcgcgct taagccgacg atcaataccg
     9721 ccacgccgag ggcaatgcca atggtagaaa tgacagagat tagcgacacc atgccgccgc
     9781 gtcgccgtcc gcgactaaaa cgcaggccaa tcaataacga taaaggcatc gccattactc
     9841 cgcccccatc aggctcagtt ccgccgtcag acgtccatca cgcatttcca gttggcggct
     9901 catacgtttc gccagttgca ggtcgtgagt gaccaccagg aaagcggtgc cctgcaagcg
     9961 attcaactcc ccaagcaact ggaagatgct gtcggcgtta cgcgcatcga ggttaccggt
    10021 aggttcatcc gccagtacca gacgcggatt attgaccagc gcacgggcaa tcgctacacg
    10081 ctggcgttcg ccgccagaaa gttcagatgg gcggtgattc gcacgatgct ccagccccac
    10141 cgcttttaac atctccagtg cacagctgtt gatttcagcg ggctttttct tgccaatcag
    10201 cagtggcata gccacgtttt ccagggcagt aaaatccggc agcaggtggt gaaactgata
    10261 aataaagccc agcttctggt tgcgcagttc tgctttcgcc gctgaagaca gtttgctcat
    10321 cggctgaccg ttaaagatca catcgccgga ggttggcgta tccagcccgc ctaacaggtg
    10381 cagcaaggta cttttaccgg aaccagagct accgacaatt gccatcattt caccttcgcc
    10441 cacgctgaaa ctgacattga gcagcacatc ggtttgcaca ctgccttcct gatagcgttt
    10501 gcacaggttg tcgcattgca acaggatctt attcataacg taaagcctca gcgggttgtg
    10561 tggcggcagc gcgccatgaa gggtaaagcg tagacagcag cgcgatagcc atcgccacca
    10621 gcgcaataac aatgacctgt aaaggttcga tagccaccgg cagcgccgcg ccatcaagca
    10681 ggacgccgat tatcggcatc agattattta actggctggc aagcagtgcg ccaagcgccg
    10741 ctccgaggat tgcaccgata atcccggcgc tagccccttg caccataaag accatcatga
    10801 tttgtcgcgg agttaagcct tgcgtttgca ggatcgcgac ttcgccctgc ttctccatca
    10861 ccatcagccc cagcgaggta ataatgttaa acgccgcaac agcgacaatc aggctcagca
    10921 gtaagcccat catatttttt tccatgcgta cggcctggaa cagctcgcct ttacggtcac
    10981 gccagtcctg ccatttgctg ccttcaggca gtttttgctg acttaaagag tcaactctca
    11041 gcggctcatc cagccacaaa cgccagccgg taatattggc tgccggataa cgcatcagac
    11101 gcgaggcatc ctcaatattc accagcattt catagccatc gacttcactg ttggcggcga
    11161 aagtgccaat cacattgaac aggcgctgac ttggaatacg ccccatcggc gtgaactggc
    11221 tggcagatgg caccatcacg cggatttgat caccgcgatt gacgcccaac tgtgaggcaa
    11281 gttgttcgcc gaggatgaca ttatatttcc ccggctcgag gtcagtttgt ttcacattga
    11341 ccagatacgg cgttagtgga tctttttgcg ccgggtcgat accgagcatc accccgaccg
    11401 ccacgctgcg cgcgctttgc agtaccacat caccggtagt aataggtgcg acgcgattaa
    11461 cgccgtccag tttgactgcc gtttccggga gttgctgcgg gttaagagag ccatgctcag
    11521 aagagagaat tgcctgtggc atcaggccaa ggatgttgtt ttgcagctcg cgctcaaagc
    11581 cgttcatcac tgacaatact gtgaccagcg ccatcacccc gagggtaatg ccgatggtag
    11641 aaagccagga gacgaaacga ccgaagcgat ccactgcacg cccacgcatg taacgcaggc
    11701 caataaatag agcgacaggt tggtacatga aatccgtctg gttgctgtag caaagacacg
    11761 agtatataag cgaaagcaag atgggtaaac gtctgaactg taagtgatcg tcagggctaa
    11821 tctgaaaagc ccccgtaaat caatcggcta aattgccata ttttatcatc aatcttgccg
    11881 cctgatctta atctgaaaaa tcatccaata cagacagata cccattacac ctcgggtcat
    11941 aaccagcagg atcaaccgca tgtaaattaa aaaagagcgt gttgttctga tgaaacaaaa
    12001 agagctatgg attaaccaga tcaaagggtt atgtatttgt ctggtggtga tttatcactc
    12061 ggtcattacc ttttatccgc atatgaccac ttttcagcat ccgttatcgg aagtcctgag
    12121 caaatgctgg atctatttca atctttacct tgcccccttt cgtatgccgg tttttttctt
    12181 tatatccggc tatttgattc gccgctatat cgacagcgta ccgtggggaa attgtctcga
    12241 taaacggatc tggaacatct tctgggtgct ggcgctttgg ggcgtggtgc agtggctggc
    12301 gttaagtgcg ctgaaccagt ggctggcacc tgagcgcgat ttaagcaatg cctccaatgc
    12361 cgcttatgcc gattccaccg gtgagttcct gcacgggatg atcaccgcca gcaccagctt
    12421 gtggtatctg tatgcgttaa ttgtctattt cgtggtatgt aaaattttta gccgtctggc
    12481 gctgccacta ttcgccttgt ttgtactgct gagtgtggcg gttaatttcg ttcccacgcc
    12541 gtggtgggga atgaacagtg tgatacgcaa tttgccttat tacagccttg gcgcatggtt
    12601 tggcgcaaca ataatgacct gtgttaaaga ggtgccgttg cgccgccatc tgctgatggc
    12661 ttctttgctt accgttctgg cggtcggtgc ctggttgttt actatctcgc tgctgttgtc
    12721 gctggtatcg attgtggtga tcatgaagct gttttatcag tacgagcaac gattcggtat
    12781 gcgctccacc agcctgctga atgtgattgg ctccaacacc attgctatct acaccaccca
    12841 tcgcattctg gttgaaatat tcagcttaac tctgcttgcg caaatgaacg cagcacgctg
    12901 gtcgccgcaa gtcgaactga cactcctgct ggtttatccc tttgtcagtt tgttcatctg
    12961 tactgttgcg ggcttgctgg taagaaaact ttcacagcgt gcattcagcg atctgttgtt
    13021 ctccccgcct tcactgcccg ctgccgtcag ttactctcgt taaaccatca ccccagccgg
    13081 ggcgattcaa tttgctaaac catgtcgatt acggataatg aacagcattg cttatcagag
    13141 atatgccccc atatgttgag gcatatccta acgagaatct gacaaccgtt atgcctgaac
    13201 aatatcgtta tacgctgccc gtcaaagcgg gtgagcagcg tctgctgggc gagttaaccg
    13261 gcgcagcctg tgcaacgctg gtagcggaaa ttgccgaacg tcacgccggt ccggtggtac
    13321 tcattgcacc agatatgcaa aatgctctgc gtttgcatga tgaaatcagc cagttcaccg
    13381 atcagatggt gatgaatctg gcggactggg aaactcttcc ctacgacagt ttttcgcctc
    13441 atcaggacat tatctcctcg cgcctttcca ccctttacca gctaccgacg atgcagcgtg
    13501 gcatactgat tgttccggtg aatacgctta tgcagcgcgt ttgcccacac agttttctcc
    13561 acggtcatgc gctggtgatg aaaaaaggtc agcgcctgtc acgagatgca ttacgaaccc
    13621 aactggacag cgccggttat cgccatgttg accaggtgat ggagcacggc gaatacgcca
    13681 cgcgcggcgc gttgctggat ctcttcccga tggggagtga gctgccttat cgtcttgatt
    13741 tctttgatga tgaaatcgac agcctgcggg tgtttgacgt cgacagccag cgcacgctgg
    13801 aggaagtaga agcgatcaat ctgctgcccg cgcacgaatt tccgaccgat aaagcggcaa
    13861 ttgaactgtt ccgcagccag tggcgcgata ccttcgaagt gaagcgcgat ccggaacata
    13921 tttaccagca agtgagtaaa ggcacattac ctgctgggat cgagtactgg cagccattgt
    13981 tcttcagcga accactgcca ccgctgttca gttatttccc tgccaatacc ttgctcgtga
    14041 atactggcga tctggaaacc agtgccgaac gtttccaggc tgacacgctg gcgcgttttg
    14101 agaatcgcgg cgtcgatccg atgcgcccgc tgttgccacc acaatcgctc tggctgcggg
    14161 tggacgagct cttctcagag ctgaaaaact ggccccgagt gcagctaaaa actgaacatt
    14221 taccgacaaa agccgcgaat gccaatttag gtttccagaa actgccagac ctggccgttc
    14281 aggcgcaaca aaaagcgccg ctggatgcgc tgcgtaagtt cctcgagact ttcgacggtc
    14341 cggtggtgtt ctcggtagaa agtgaaggtc gccgtgaagc gctgggtgaa ctgctcgcac
    14401 gaattaaaat tgctccgcaa cgcattatgc gtcttgatga agccagcgac cgtgggcgtt
    14461 atctgatgat tggcgctgcc gaacatggtt ttgtcgataa ggcgcgtaat ctggcgctga
    14521 tctgcgaaag cgatctgctc ggtgaacgtg ttgcccgccg tcgtcaggat tctcgccgca
    14581 ccatcaaccc cgatacactg atccgtaacc ttgcggagct gcatattggt cagccggtgg
    14641 tccatctgga gcacggcgtc ggtcgctatg ccggaatgac cacgctggaa gctggcggca
    14701 ttactggcga gtacctgatg ctcacctatg ccaacgacgc caaactgtat gttccggtgt
    14761 cgtcactgca tctgattagc cgttacgcag gtggcgcgga agaaaacgcc ccgctgcata
    14821 aacttggcgg cgatgcgtgg tcacgcgcgc ggcagaaagc ggcggaaaaa gtgcgtgatg
    14881 tggcggcgga attgctggat atctacgcgc aacgcgccgc caaagagggc ttcgcgttta
    14941 aacacgatcg tgagcagtat cagttattct gcgacagctt cccgtttgaa accacgccgg
    15001 atcaggcgca ggccattaat gcggtactta gcgacatgtg tcagccgctg gcaatggatc
    15061 gtctggtgtg cggcgatgtt ggctttggta aaacagaagt ggcgatgcgc gccgctttcc
    15121 tggcagtaga taaccacaag caggtagcgg tgttggtgcc taccaccctt cttgcgcagc
    15181 agcattacga caacttccgc gaccgtttcg ccaactggcc ggtacgtatc gaaatgatct
    15241 cccgtttccg cagcgccaaa gagcagacgc aaatccttgc ggaagtggcg gaagggaaaa
    15301 tcgatattct gatcggtacg cacaaactgc tgcaaagtga cgtcaagttt aaagatttag
    15361 gcctgctgat tgtcgatgaa gaacaccgct tcggggtgcg tcataaagag cgcattaaag
    15421 cgatgcgcgc gaacgtggat attctgacgc ttactgcaac gccgatccca cgtacgctga
    15481 atatggcaat gagcggaatg cgtgacctgt cgattatcgc cacgccgccc gcccgtcgtc
    15541 tggcagttaa aacctttgtc cgtgagtatg acagcctggt ggtccgggag gcgatcctgc
    15601 gtgaaatttt gcgcggagga caggtttatt atctctacaa tgatgtggaa aacattcaga
    15661 aagccgccga acggctggca gaactggtgc ctgaagcacg gattgccatc ggtcacgggc
    15721 agatgcgcga gcgcgaactg gaacgggtga tgaatgattt ccatcatcaa cgtttcaacg
    15781 tgctggtttg taccaccatt atcgaaaccg ggatcgacat cccgacagcc aacaccatta
    15841 tcattgaacg tgcggatcac ttcggtctgg cgcagctgca ccagttacgc ggtcgcgtcg
    15901 gacgttcaca tcatcaggca tatgcatggc tgctgacgcc gcatccaaaa gcgatgacta
    15961 ccgatgcaca aaaacgtctt gaagcgattg cctcgctgga agatctcggg gcaggttttg
    16021 cgctggcaac gcacgatctg gagattcgcg gcgcgggtga actgcttggc gaagaacaaa
    16081 gcggctcaat ggaaaccatc ggtttctcgc tgtatatgga gttgctggaa aacgccgtcg
    16141 atgcactgaa agccggacgc gagccgtcgc tggaagatct caccagccag caaacagaag
    16201 tcgagctgcg gatgccgtcg ctattgccag atgatttcat ccctgacgtg aacacgcgtc
    16261 tgtcgttcta caaacgtatt gccagcgcca aaacggaaaa cgaactggaa gagatcaaag
    16321 tcgagcttat cgatcgcttc ggcctgctgc cagatccggc gcgtaccctg ctggatattg
    16381 cccgtctgcg ccagcaagcg cagaaactgg ggatcaggaa gctggaaggt aatgagaaag
    16441 gcggggtgat cgaatttgcc gagaagaatc acgttaatcc ggcctggttg attggtttgc
    16501 tgcaaaaaca gccgcagcat taccgccttg atggtccgac gcgcctgaaa tttattcagg
    16561 atttgagtga gcggaaaacg cgtatcgaat gggtacgcca gtttatgcgt gaactggaag
    16621 agaacgcgat cgcttaatgt aaatcggggg cgtttctgcg cccccatctg caacatttac
    16681 aaattctttg cactttcctg cactatccga cactgtcacc atccataatt caggcttatc
    16741 tgtttattac aataacctta tatttattat gtatttttgg tgatgatcaa aacgcgtttt
    16801 tctcgctggc taacgttttt tacgttcgcc gctgccgtgg cgctggcgct accggcaaaa
    16861 gccaacacct ggccgctgcc gccagcgagc agtcgtctgg ttggcgaaaa caaatttcat
    16921 gtggtggaaa atgacggtgg ttctctggaa gccatcgcca aaaaatataa cgtcggcttt
    16981 ctcgctctgt tacaagctaa ccccggcgtt gatccttacg taccgcgcgc gggcagcgtg
    17041 ttaacgatcc cgttgcaaac cctacttcca gacgcgccgc gcgaaggcat tgtgatcaac
    17101 attgcggagc tgcgtctcta ttactacccg ccgggtaaaa attcggtaac cgtgtatcca
    17161 ataggtattg gtcagttagg tggtgacacg ctgacaccga caatggtgac caccgtttca
    17221 gacaaacgtg caaacccaac ctggacgcca acggcaaaca tccgcgcccg ttataaagca
    17281 cagggaattg agttgcctgc ggtagtgccg gctggaccgg ataacccaat gggccatcat
    17341 gcgattcgtc tggcggccta tggcggcgtt tatttgcttc acggtacgaa cgccgatttc
    17401 ggcattggca tgcgggtaag ttctggctgt attcgtctgc gggatgacga tatcaaaaca
    17461 ctctttagcc aggtcacccc aggcaccaaa gtgaatatca tcaacactcc gataaaagtc
    17521 tctgccgaac caaacggtgc gcgtctggtt gaagtacatc agccgctgtc agagaagatt
    17581 gatgacgatc cgcagctgct gccaattacg ctgaatagcg caatgcaatc atttaaagat
    17641 gcagcacaaa ctgacgctga agtgatgcaa catgtgatgg atgtccgttc cgggatgccg
    17701 gtggatgtcc gccgtcatca agtgagccca caaactctgt aaatcctgga taaaaaaaat
    17761 ccccgcgaca cggcatgtcg cggggaaaaa atcagcagta gcaggcatta atgagggtta
    17821 atgcttattt ataaattact gctgttccat ggagggtatt cggaccggtt acagaagtaa
    17881 tacggaaaga ttttgcgccc atctcatccg ctttttgcgc cagctgctct tccagcgatc
    17941 ccagatttgt ccccgcgtta gcactgatag taccgacttt ttgttggcct tctggcgttg
    18001 actgaacttc gacagccgca aagttggcaa atgacatgga gcttaaaatc gccgcagcga
    18061 tgaggttttt acgtttttca taatagtggc cttatgcaga tgaatgacgt cgcaagaaat
    18121 tacttaacga tcgataacta
//
LOCUS       DAZPAB010000072        17941 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZPAB010000072 DAZPAB010000000
VERSION     DAZPAB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.072"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1..1045
                     /locus_tag="ACHUPG_002133"
                     /pseudo
     CDS             1..1045
                     /locus_tag="ACHUPG_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(1191..2228)
                     /gene="iap"
                     /locus_tag="ACHUPG_002134"
     CDS             complement(1191..2228)
                     /gene="iap"
                     /locus_tag="ACHUPG_002134"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR0861746.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            2480..3388
                     /gene="cysD"
                     /locus_tag="ACHUPG_002135"
     CDS             2480..3388
                     /gene="cysD"
                     /locus_tag="ACHUPG_002135"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR0861747.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            3390..4817
                     /gene="cysN"
                     /locus_tag="ACHUPG_002136"
     CDS             3390..4817
                     /gene="cysN"
                     /locus_tag="ACHUPG_002136"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR0861748.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            4817..5422
                     /gene="cysC"
                     /locus_tag="ACHUPG_002137"
     CDS             4817..5422
                     /gene="cysC"
                     /locus_tag="ACHUPG_002137"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR0861749.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            5472..5795
                     /locus_tag="ACHUPG_002138"
     CDS             5472..5795
                     /locus_tag="ACHUPG_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR0861750.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            5989..6300
                     /gene="ftsB"
                     /locus_tag="ACHUPG_002139"
     CDS             5989..6300
                     /gene="ftsB"
                     /locus_tag="ACHUPG_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR0861751.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            6319..7029
                     /gene="ispD"
                     /locus_tag="ACHUPG_002140"
     CDS             6319..7029
                     /gene="ispD"
                     /locus_tag="ACHUPG_002140"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR0861752.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            7029..7508
                     /gene="ispF"
                     /locus_tag="ACHUPG_002141"
     CDS             7029..7508
                     /gene="ispF"
                     /locus_tag="ACHUPG_002141"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR0861753.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            7505..8554
                     /gene="truD"
                     /locus_tag="ACHUPG_002142"
     CDS             7505..8554
                     /gene="truD"
                     /locus_tag="ACHUPG_002142"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR0861754.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            8535..9296
                     /gene="surE"
                     /locus_tag="ACHUPG_002143"
     CDS             8535..9296
                     /gene="surE"
                     /locus_tag="ACHUPG_002143"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR0861755.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            9290..9916
                     /gene="pcm"
                     /locus_tag="ACHUPG_002144"
     CDS             9290..9916
                     /gene="pcm"
                     /locus_tag="ACHUPG_002144"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR0861756.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            10101..11195
                     /gene="nlpD"
                     /locus_tag="ACHUPG_002145"
     CDS             10101..11195
                     /gene="nlpD"
                     /locus_tag="ACHUPG_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR0861757.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            11258..12250
                     /gene="rpoS"
                     /locus_tag="ACHUPG_002146"
     CDS             11258..12250
                     /gene="rpoS"
                     /locus_tag="ACHUPG_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR0861758.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(12370..12777)
                     /locus_tag="ACHUPG_002147"
     CDS             complement(12370..12777)
                     /locus_tag="ACHUPG_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFR0861759.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            12924..13517
                     /locus_tag="ACHUPG_002148"
     CDS             12924..13517
                     /locus_tag="ACHUPG_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFR0861760.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            13517..14464
                     /locus_tag="ACHUPG_002149"
                     /pseudo
     CDS             13517..14464
                     /locus_tag="ACHUPG_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(14737..15300)
                     /locus_tag="ACHUPG_002150"
     CDS             complement(14737..15300)
                     /locus_tag="ACHUPG_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861761.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            15465..15716
                     /locus_tag="ACHUPG_002151"
     CDS             15465..15716
                     /locus_tag="ACHUPG_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0861762.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            15718..17814
                     /locus_tag="ACHUPG_002152"
     CDS             15718..17814
                     /locus_tag="ACHUPG_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFR0861763.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         4426 a         4388 c         4818 g         4309 t
ORIGIN      
        1 aatattgtga ctttctttga tttctgggga aaaaaccgac gtggcgagaa agagggtatc
       61 gacgactatc accttctttg ctggcatttg ctggatgtgg ccgcaatggg ctatttaatg
      121 gttaaaagaa attgcttcgg gctggctgat tattttcgtc aattagggat ttctgacaag
      181 gaacaggcag ctcgattttt cgcctggttg ctgtgctggc acgatattgg aaaatttgcc
      241 cgctcttttc aacaacttta cctgccccct gaattcaaga ttcaggaagg tgccagaaaa
      301 aatctctcat tcaacgctgg gctactggtt gtggaatcat tatttaagtg aatgtcagga
      361 gttacttcct tcatcttcgc tctctccgcg caaacttagg cgagtgatag aaatgtggat
      421 gcccgtgacg acagggcatc atggtcggcc ccctgatcgt atggatgaat tggataattt
      481 tctgcctgaa gacaaagctg cagcacgaga ttttctcctt gaaatcaagc cactgtttcc
      541 gcgaatagag atccccacat tctgggatga tgacgagggc gttgaactta taaaacaact
      601 ttcctggtat atctctgcaa ccgtcgtact tgcagactgg acagggtcgt caacgcgatt
      661 ttttccacgc gtcgcacagg caatggatat tgaagattac tggcaaaagg ctttagttca
      721 ggctcaaaac gcattaaccg tctttccgcc acaagcagaa accgcgcctt tcaccggaat
      781 taatacgctg tttcctttta ttgagaatac gacaccgtta cagcaaaagg tgctggatct
      841 ggatatcaat cagccaggac cacagttgtt tattctggaa gatgtgaccg gcgcaggtaa
      901 aacagaagcg gcgcttatcc tggcgcacag gttgatggct gcgggtaacg ctcaggggct
      961 gttttttggc ctgccaacaa tggcaacggc caatgccatg tacgatcgac tggtcaaaac
     1021 ctggcttgct ttctattcgc cagaggtgtt ccccgctcca gcggagataa accggcgcaa
     1081 tacggtgcgg ttttcccgga agtaaaccgg acgcactgga tgcgatgatg ggtatcactt
     1141 agaattcccc gcccctgcgg tagaactccc agctcccatt ttcaaaccca tcaagacgcc
     1201 ttcgccagct ccttcaccaa cggtagcatt atccgcataa cgtcacggca gcgacgttct
     1261 attcttccag gaagtgcctt atcaatatgt tgttgattat ccagtcttac gtcatgccag
     1321 ctatttcccg ccgggaaggc aggtgttttt gcgcgttgct gataaccatc cttatttcca
     1381 agattccagt tagtcgcttc caccgaaagt acagcaatgc ccgctttgtc gaatatttct
     1441 gcgtcattac aacccccagt gcctttcgga taatttttat tcaaacccgg attggtcgtt
     1501 gcggctattc cgtgactgcg tgcaattgcc agcgccctgt cgcgcgttaa tttccttact
     1561 gcttcagggg tttttacacc gctgttgaaa tacaatttat cgccaacaat taagttatcg
     1621 agattaatca ccagcagcgt atttttcttt tcggtgtcac tcatccgctt gagtaaattc
     1681 tcagcgccta attttccttc ctcttcgccg ctggtcgcca caaatcgaat accatactcg
     1741 gtaggcgtat ttttcaggcg ttctgccagt tccagcatga cacctaaacc tgcggcgtta
     1801 tcatctattc cttgtaacgt cagtccgccg agattggcat ctgcgtcagc atcgctcagc
     1861 ggggcgtaag tatccagatg cgccataatg atgatctgct gcggcgcttt gccttcatga
     1921 gcggcaatca ccgtacttcc tgtcacgtta tgccagttct tgcgattatc gcgggcggta
     1981 taaatatacc gactattaaa tgtccgaata tcactgcgat aacccatttg ctgaaactgt
     2041 tggcgaatat aatcggcaga taacatttct gctggagttc cggtcatgcg tcccggaaag
     2101 aaagtagcaa tatgtcgtgc ctgggtatta gcaaaatcgc caggtttagg tgacgaggcg
     2161 tgtacgggga gaataaagca tacgccgagc gccagggcag cggtacggtg gcgcaatgcg
     2221 gaaaacatag tgagtcctta aataccatgc aaattttttt accgccatag tatgaaactg
     2281 ccgctgcgct aaaacaattt caaatcttcc taaacgcccg aaatccggtg ccttaagcac
     2341 tttttgatat tagctttgcc aaatcgttat tccgttaagg aactactcat tctaattggt
     2401 aatttcattc gttctcttac gctccctata gtcgaaacat ctgatggcaa gaaaatagcg
     2461 gtattgcaaa ggaacggtta tggctcaaaa acgacttact cacctgcggc aactggaggc
     2521 ggaaagcatc cacattattc gcgaggtggc ggcagaattc tcaaatccgg tgatgctcta
     2581 ctctatcggt aaagattcca gcgtcatgct gcatctggcg cgcaaggcgt tttatccagg
     2641 tacgctgcct ttcccgttgc tgcatgtcga taccggctgg aaattccgcg agatgtatga
     2701 gttccgcgat cgtacggcga aagcctacgg ctgcgagctg ctggtgcata aaaacccgga
     2761 aggcgtggcg atggggatta atccattcgt gcacggcagc gcgaaacata ccgatattat
     2821 gaaaactgaa ggcctgaaac aggcgctgaa caaatacggt tttgatgccg ccttcggtgg
     2881 tgcgcgccgt gacgaagaga aatcccgcgc taaagagcga atctactctt tccgtgaccg
     2941 cttccatcgc tgggatccga aaaatcagcg cccggagctg tggcacaact acaacgggca
     3001 aattaacaaa ggcgaaagca tccgcgtctt cccgctctct aactggaccg agcaggatat
     3061 ctggcaatac atctggctgg aaaatatcga cattgtgccg ctgtatctcg ctgcggaacg
     3121 tccggttctg gaacgcgacg gtatgttgat gatgattgat gacaaccgta tcgacctgca
     3181 accgggcgaa gtgatcaaaa aacggatggt gcgtttccgt acgctgggct gctggccgct
     3241 gaccggtgcg gtggagtcaa atgcacaaac actgccggaa atcatcgaag agatgctggt
     3301 ttccaccacc agtgaacgtc agggtcgcgt gattgaccgc gaccaggcgg gatccatgga
     3361 gctgaaaaaa cgtcaggggt atttttaaga tgaacaccgt acttgcacaa caaatcgcca
     3421 atgaaggcgg cgtcgaagcc tggatgattg cgcaacaaca taaaagcctg ctgcgttttc
     3481 tgacctgtgg tagcgtcgat gacggcaaaa gtaccctgat tggtcgtctg ctgcacgata
     3541 cccgccaaat ctacgaagat cagctctcat cgctgcataa cgacagtaag cgtcacggaa
     3601 cccagggcga aaagctggat ctggctctgc tggtggacgg cctgcaagct gagcgcgaac
     3661 agggcatcac tattgatgtg gcctaccgct atttctctac cgagaagcgt aaatttatta
     3721 tcgccgacac cccagggcac gagcagtaca ctcgcaatat ggcgactggc gcatcgacat
     3781 gtgaactggc gatcttactg attgatgccc gtaaaggcgt gctcgatcaa acccgtcgtc
     3841 acagttttat ctccacactg ttggggatca aacatctggt cgtggcgatc aacaaaatgg
     3901 atctggtgga ttacagtgaa gagacgttca cccgtattcg tgaagattat ctgacctttg
     3961 ccgggcagct gccgggtaat ctggatatcc gctttgtgcc actctccgca ctggaaggtg
     4021 acaacgtggc ttcgcaaagt gaaagtatgc cgtggtacag cggtctgaca ctgctcgaag
     4081 tgctggaaac cgtggagatc cagcgagtgg tggatgccca gccaatgcgc ttcccggtgc
     4141 agtacgttaa ccgcccaaat ctcgattttc gtggctacgc cggaatgctg gcatccggtc
     4201 gcgtggaagt cgggcaacgt gtaaaagtgc tgccctctgg tgtggaatca aacgtcgcgc
     4261 ggatcgtgac ttttgatggt gatcgcgaag aagtctttgc cggagaagcg atcaccctgg
     4321 tgctgacgga tgagatcgat atcagccgtg gtgatctgct gctggcggca gacgaagcgt
     4381 taccggcggt gcagagtgcg tcggtggatg tggtatggat ggcggaacag ccgctttccc
     4441 cgggccagag ttatgacatc aaaattgccg gtaagaagac gcgtgctcgt gttgatggca
     4501 ttcgttatca ggttgatatt aataacctta cccaacgcga agttgaaaac ctgccgctga
     4561 acggcatcgg cctggtggat ctcacttttg acgagccact ggtgttagat cgctatcagc
     4621 aaaatccggt taccggtggg ctgattttta tcgatcgcct gagcaatgtg accgtgggtg
     4681 ccggtatggt gcacgagcca gttagccagg caacagctgc gtcatctgaa ttcagtgcat
     4741 tcgaactgga attgaatgcc ctggttcgtc gccactttcc gcactggggc gcgcgcgatt
     4801 tgctggggga taaataatgg cgctgcatga cgaaaacgtc gtctggcata gtcatccggt
     4861 cactgtgcaa caacgcgaac tacaccacgg tcatcgtggt gtagtgctgt ggtttaccgg
     4921 cctctccggg tccggtaaat caacggtcgc cggggcgctg gaggaggcgt tacataaact
     4981 cagcgtcagt acgtatctgc tggatggcga caatgttcgc cacggattat gcagcgatct
     5041 cggttttagc gatgccgatc gtaaagagaa tatccgtcgc gtcggtgaag tggcgaattt
     5101 gatggttgaa gccggactgg tggtgctgac cgcatttatc tcgccacacc gcgccgaacg
     5161 ccagatggtt cgcgaacgcg taggagaagg gcgctttatt gaagtgtttg tcgatacgcc
     5221 gctggcgatt tgcgaagccc gcgatccaaa aggcttatat aagaaagcgc gtgccggtga
     5281 actgcgcaac tttacgggaa tagattccgt ttacgaagcg cctgaatcgg cagaaattca
     5341 tctcaatggt gaacaattag taacaaattt ggtacagcaa ttattagatc tgttgagaca
     5401 gaacgatatt atcagatcct gagacaccac cgggtttcca tgcccggttc gtcaaagtaa
     5461 caggatcaaa tatgcgtaat agccataaca ttacactaac aaataacgac agccttactg
     5521 aggatgaaga aactacatgg tcactgcctg gtgccgtggt cggttttatc tcctggttat
     5581 ttgcgctggc gatgccgatg ttgatttatg gctctaacac gctgttcttc tttctctaca
     5641 cctggccttt ctttctggca ctgatgcctg tcgcggtagt ggtggggatt gcgctgcatt
     5701 cattgatgga cggaaagcta cgctacagta ttgttttcac cctggtgaca gtaggcatta
     5761 tgtttggcgc actgtttatg tggctactgg gctaatttgt actttcttgt ctcctctgtt
     5821 cataattcaa accgtaacta ataatgagat tatgttctgc acgccctggg tatacgtaac
     5881 aatgggcaaa tgtggtacat ttgcccgcgt tgtcgcggta tccccaacag aggatgtaga
     5941 gtcgtcttcg gatgcatggg atgatgatgc agtttttcag ggggcaggat gggtaaacta
     6001 acgctgctgt tgctggctat tctggtctgg ctacagtatt cgctgtggtt cggtaagaac
     6061 ggtatacatg actatacccg cgtcaatgat gatgtggcgg cactgcaagc tacaaacgcg
     6121 aaacttaaag cgcgaaacga tcaacttttt gccgaaattg acgatctcaa tggcggccag
     6181 gaggcgctcg aagagcgtgc gcgtaatgaa ctcagcatga ccaggccggg cgaaactttt
     6241 tatcgtctgg tgcctgacgc gtcgaagcgc gcacagtctg cggggcaaaa caatcgataa
     6301 atcagcccgg gaattaacat ggcaaccact catttggatg tttgcgccgt ggttccggcg
     6361 gccggatttg gccgtcgaat gcaaacggaa tgtcctaagc aatatctctc aatcggtaat
     6421 caaaccattc ttgaacactc ggtgcatgcg ctgctggcgc atccccgggt gacacgtgtc
     6481 gtcattgcca taagtcctgg cgatagccgt tttgcacaac ttcctctggc gaatcatccg
     6541 caaatcaccg ttgtagatgg cggtgatgag cgtgccgatt ccgtgctggc aggtctgaaa
     6601 gccgctggcg atgtgcagtg ggtattggtg catgacgccg ctcgtccttg tttgcatcag
     6661 gatgaccttg cgcgattgtt ggcgttgagc gaaaccagcc gcacgggagg gatcctcgcc
     6721 gcaccagtgc gcgatactat gaaacgtgcc gaaccgggca aaaatgccat tgctcatacc
     6781 gttgatcgca acggcttatg gcacgcgctg acgccgcaat ttttccctcg tgagctgtta
     6841 catgactgtc tgacgcgcgc tctaaatgaa ggcgctacca ttaccgacga agcctcggcg
     6901 ctggaatatt gcggatttca tccgcagttg gtcgaaggcc gcgcggataa cattaaagtc
     6961 acgcgcccgg aagatttggc actggccgag ttttacctca cccgaaccat ccatcaggag
     7021 aatacataat gcgaattgga cacggttttg acgtacacgc ctttggcggt gaaggcccaa
     7081 ttatcattgg tggcgtacgc attccttacg aaaaaggatt gctggcgcat tctgatggcg
     7141 acgtggcgct tcatgcgttg accgatgcat tgcttggcgc ggcggcgctg ggggatatcg
     7201 gcaaactgtt cccggatacc gatccggcat ttaaaggtgc cgacagccgc gagctgctac
     7261 gcgaagcctg gcgtcgtatt caggcgaagg gttataccct tggcaacgtc gatgtcacta
     7321 tcatcgctca ggcaccgaag atgttgccgc acattccaca aatgcgcgtg tttattgccg
     7381 aagatctcgg ctgccatatg gatgatgtta acgtgaaagc cactactacg gaaaaacttg
     7441 gatttaccgg acgtggggaa gggattgcct gtgaagcggt ggcgctactc attaaggcaa
     7501 caaaatgatt gagtttgata atctcactta cctccacggt aaaccgcaag gcaccgggct
     7561 gctgaaagcc aatccggaag actttgtggt ggtggaagat ttgggctttg agcctgatgg
     7621 tgaaggtgag catattctgg ttagaatcct caaaaacggc tgcaataccc gttttgtggc
     7681 ggatgcactg gcgaaattcc tgaaaattca ttcccgtgaa gtcagcttcg ctgggcaaaa
     7741 agacaaacat gctgtaacgg aacagtggtt atgtgctcgc gtgccgggca aggaaatgcc
     7801 cgatctgagc gcctttcaac tggaaggctg ccaggtgctg gagtatgcgc ggcacaagcg
     7861 caagctgcgt ttaggggcgc tgaaaggtaa cgcctttacc ctggttctgc gcgaagtgag
     7921 caatcgcgat gacgttgaac aacgtctgat cgatatttgc gtaaaaggtg taccgaacta
     7981 cttcggtgcc caacgttttg ggattggcgg tagcaacttg cagggcgcgc tgcgctgggc
     8041 gcaaaccaat actccggtgc gcgatcgcaa taaacggagt ttttggttgt cggcagcccg
     8101 cagtgcgttg tttaatcaga ttgttgctga gcgcctcaaa aaagcagacg ttaatcaagt
     8161 tgttgacggc gatgcgctac aattagccgg acgtggtagc tggtttgtag caaccaccga
     8221 agaactggcg gaattacagc gtcgcgtcaa cgataaagag ctgatgataa ccgccgcatt
     8281 gccgggcagt ggcgaatggg gaactcagcg tgaagcgctg gcattcgaac aagcagctgt
     8341 cgccgcagaa actgaattac aagctttact ggtgcgcgaa aaagttgaag ccgcgcgcag
     8401 agcgatgctg ctgtatccgc aacaattaag ctggaactgg tgggatgacg tcaccgtaga
     8461 gatccgtttc tggcttccgg cgggtagttt tgcaaccagc gttgtcaggg aacttatcaa
     8521 cacaacaggt gattatgcgc atattgctga gtaattatga cggggtacat gcacccggta
     8581 tacaaacgct ggcgaaagcc ttgcgtgagt ttgctgacgt tcaggtggtc gcccccgatc
     8641 gtaaccgcag cggcgcttca aattctctga cactggaatc ctccctgcgc acgtttacct
     8701 ttgaaaatgg tgatattgct gtgcaaatgg gaaccccgac cgattgtgtc tatcttggcg
     8761 tgaatgctct gatgcgtccg cgcccggaca ttgttgtgtc cggaattaac gccgggccga
     8821 atctggggga tgatgttatt tattccggta cggtagccgc cgcgatggaa ggccgtcatt
     8881 taggttttcc ggcgctggcc gtctcgcttg acgggcataa acattacgac actgccgcgg
     8941 cggtaacctg ttcaattttg cgcgcactgt gtaaagagcc gctgcgcacc ggacgtattc
     9001 ttaatattaa cgttcaggat ttacccttgg atcaaatcaa aggtattcgc gtgacgcgct
     9061 gcggtacacg acatccggca gatcaggtga tcccgcagca agatccgcgc ggcaatacgc
     9121 tgtactggat tggcccgccg ggcggtaaat gtgatgccgg tccggggacc gattttgctg
     9181 cggtagatga gggctatgtc tccatcacgc cgctgcatgt ggatttaact gcgcatagcg
     9241 cgcaagatgt ggtttcagac tggttaaaca gcgtgggagt tggcacgcaa tggtaagcag
     9301 acgcgtacaa gcacttctgg atcaattacg tgcgcaaggt attcaggatg agcaggtgct
     9361 gaatgcactt gccgccgtgc cgcgtgaaaa attcgttgat gaagcgtttg aacaaaaagc
     9421 ctgggacaat atcgccttgc cgataggtca ggggcagaca atttcgcagc cgtatatggt
     9481 ggcgcgaatg accgaattac tcgagctgac gccgcagtcg cgggtgctgg aaattggcac
     9541 tggttcggga tatcaaacgg caatcctggc gcatcttgtc cagcatgttt gctcggttga
     9601 acggattaaa ggcttgcagt ggcaggcacg tcgccgcctg aaaaatcttg atttacataa
     9661 tgtttcaacc cgtcatggtg atggatggca aggttggcag gcacgtgcgc cgtttgacgc
     9721 tatcattgtt acggcggcac cgccggaaat tccaactgcg ctaatgacgc agctggacga
     9781 aggcgggatt ctcgtcttac ccgtagggga ggagcaccag tatttgaaac gggtgcgtcg
     9841 tcggggaggc gaatttatta tcgataccgt ggaggccgtg cgctttgtcc ctttagtgaa
     9901 gggtgagctg gcttaaaacg tgaggaaata cctggatttt tcctggttat tttgccgtag
     9961 gtcagcgtat cgtgaacatc ttttccagtg ttcagtaggg tgccttgcac ggtaattatg
    10021 tcactggtta ttaaccaatt tttcctgggg gataaatgag cgcgggaagc ccaaaattca
    10081 ccgttcgccg cattgcggct ttgtcactgg tttcgctatg gctggcaggc tgttctgaca
    10141 cttcaaatcc accggcaccg gtcagctccg ttaatggcaa tgcgcctgca aatactaatt
    10201 ctggtatgtt gattacgccg ccgccgaaaa tggggacgac gtctacagcg cagcaaccgc
    10261 aaattcagcc ggtacagcag ccacaaattc aggctactca acaaccgcaa atccagccag
    10321 tgcagccagt agctcagcag ccggtacaga tggaaaacgg acgcatcgtc tataaccgtc
    10381 agtatgggaa cattccgaaa ggcagttata gcggcagtac ctataccgtg aaaaaaggcg
    10441 acacactttt ctatatcgcc tggattactg gcaacgattt ccgtgacctt gctcagcgca
    10501 acaatattca ggcaccatac gcgctgaacg ttggtcagac cttgcaggtg ggtaatgctt
    10561 ccggtacgcc aatcactggc ggaaatgcca ttacccaggc cgacgcagca gagcaaggag
    10621 ttgtgatcaa gcctgcacaa aattccaccg ttgctgttgc gtcgcaaccg acaattacgt
    10681 attctgagtc ttcgggtgaa cagagtgcta acaaaatgtt gccgaacaac aagccaactg
    10741 cgaccacggt cacagcgcct gtaacggtac caacagcaag cacaaccgag ccgactgtca
    10801 gcagtacatc aaccagtacg cctatctcca cctggcgctg gccgactgag ggcaaagtga
    10861 tcgaaacctt tggcgcttct gaggggggca acaaggggat tgatatcgca ggcagcaaag
    10921 gacaggcaat tatcgcgacc gcagatggcc gcgttgttta tgctggtaac gcgctgcgcg
    10981 gctacggtaa tctgattatc atcaaacata atgatgatta cctgagtgcc tacgcccata
    11041 acgacacaat gctggtccgg gaacaacaag aagtgaaggc ggggcaaaaa atagcaacca
    11101 tgggtagcac cggaaccagt tcaacacgct tgcattttga aattcgttac aaggggaaat
    11161 ccgtaaaccc gctgcgttat ttgccgcagc gataaatcgg cggaaccagg cttttgcttg
    11221 aatgttccgt caagggatca cgggtaggag ccaccttatg agtcagaata cgctgaaagt
    11281 tcatgattta aatgaagatg cggaatttga tgagaacgga gttgaggttt ttgacgaaaa
    11341 ggccttagta gaagaggaac ccagtgataa cgatttggcc gaagaggaac tgttatcgca
    11401 gggagccaca cagcgtgtgt tggacgcgac tcagctttac cttggtgaga ttggttattc
    11461 accactgtta acggccgaag aagaagttta ttttgcgcgt cgcgcactgc gtggagatgt
    11521 cgcctctcgc cgccggatga tcgagagtaa cttgcgtctg gtggtaaaaa ttgcccgccg
    11581 ttatggcaat cgtggtctgg cgttgctgga ccttatcgaa gagggcaact tggggctgat
    11641 ccgcgcggta gagaagtttg acccggaacg tggtttccgc ttctcaacat acgcaacctg
    11701 gtggattcgc cagacgattg aacgggcgat tatgaaccaa acccgtacta ttcgtttgcc
    11761 gattcacatc gtaaaggagc tgaacgttta cctgcgaacc gcacgtgagt tgtcccataa
    11821 gctggaccat gaaccaagtg cggaagagat cgcagagcaa ctggataagc cagttgatga
    11881 cgtcagccgt atgcttcgtc ttaacgagcg cattacctcg gtagacaccc cgctgggtgg
    11941 tgattccgaa aaagcgttgc tggacatcct ggccgatgaa aaagagaacg gtccggaaga
    12001 taccacgcaa gatgacgata tgaagcagag catcgtcaaa tggctgttcg agctgaacgc
    12061 caaacagcgt gaagtactgg cacgtcgatt cggtttgctg gggtatgaag cggcaacact
    12121 ggaagatgta ggtcgtgaaa ttggcctcac ccgtgaacgt gttcgccaga ttcaggttga
    12181 aggcctgcgc cgtttgcgcg aaatcctgca aacgcagggg ctgaatatcg aagcgctgtt
    12241 ccgcaagtaa gtaagcatct gtcagaaagg ccagtctcaa gcgaggctgg cctttttctt
    12301 ttgtttggga agcttgtgtt tttgtcgcct ggataagaca cgtcagcgtc ctacccggca
    12361 atcagcattc tatgtgctca tcatcttgcg caccaactgc ataaacagct cctgctcttc
    12421 tccactcaaa cgccccaaaa attcctcatc cacgctgtca ccaataggta tcgccgcagc
    12481 aagtagcttt tctccttcgg cagtcagcca gacaaagcgt cgccgcttgt cagcgacgtc
    12541 atgttcacgt ctgactaagc cacgattttc cattcttgcc aacatttctg ctagcgttgc
    12601 tttggtgctg actgctgctt ctatcagtgc cacctgttca ataccaggtt tatcagcaat
    12661 ggcgcgcatc accgcgtact gtggtttggt caggtcaggt aactcgtgct gccagcgagc
    12721 cgtgtgctgc tgaaaaagct gtcgtaactg atggaacgct ttatttcgta acgccatgta
    12781 aactccgggc taattctctg tcgctatcat agcggttttt tagtgacgaa gaagatgttg
    12841 ttacttttca atccttgccg ctgtggaaat gaaggagtat gttaataata acgttcgtat
    12901 acgaataatt aagagaataa gcaatgaaac tgatcgtcgg gatgacaggg gctaccggtg
    12961 cgcctcttgg tgtggcatta ctgcaagcgc tgcgtgagat gccgaatgtc gagactcatc
    13021 tggtgatgtc gaagtgggcg aaaaccacca ttgaactgga aacgccttac agcgctcgcg
    13081 atgttgctgc cctcgcagac ttcagccata acccggcgga tcaggcggcg atcatctcat
    13141 ccggttcttt tcgtaccgac ggcatgatcg ttattccgtg cagtatgaaa acgctcgccg
    13201 gtatccgcgc tggttacgcc gatggcctgg tagggcgcgc ggcggacgtc gtgctcaaag
    13261 aaggccgcaa actggtgctg gtgccgcgtg aaatgccgct tagcaccatc catctcgaaa
    13321 atatgctcgc actttcacgc atgggcgtgg cgatggtgcc gccgatgcct gccttttata
    13381 accatcccga aacggtagat gacattgtcc accatgtggt agcccgcgtg ctggatcaat
    13441 ttggcctcga acatccccac gccaggcgct ggcaaggatt gccgcaggcc cggaattttt
    13501 ctcaggagaa tgaataatgg catttgatga tttacgcagc tttttacagg cgcttgatga
    13561 ccacggccag ttactgaaaa tcagcgaaga agtgaacgct gagccggatc tggcagcagc
    13621 agctaacgcc accgggcgta tcggcgacgg cgcgcccgcg ctgtggtttg ataatattcg
    13681 cggctttacc gatgcccgcg tggcgatgaa taccatcggt tcctggcaga accacgcgat
    13741 ttcccttggc ctgccaccaa acaccccggt caaaaagcag attgatgagt ttatccgccg
    13801 ctgggataac ttcccgattg ccccggagcg ccgcgccaat ccagcctggg cgcagaacac
    13861 cgtcgatggc gaagagatca acctgttcga tattctgccg ctgtttcgcc tgaatgatgg
    13921 cgatggcggt ttctatctcg acaaagcgtg cgtggtttcc cgcgatccgc tcgacccgga
    13981 taacttcggc aagcagaacg tcggcatcta ccgcatggaa gtgaagggca agcgtaagct
    14041 cggcctgcaa ccggtgccga tgcacgatat cgccctgcat ctgcataaag cagaagagcg
    14101 cggtgaagat ctgccgattg cgatcacgct cggtaacgat ccgatcatca cgctgatggg
    14161 ggccacgccg ctgaaatacg atcagtctga gtatgaaatg gcaggcgcgc tgcgtgaaag
    14221 cccgtacccg atcgccaccg ctccgctgac cggttttgat gtgccgtggg gttcagaagt
    14281 gatcctcgaa ggggttatcg aaagccgtaa acgcgaaatc gaagggccgt tcggtgagtt
    14341 taccgggcac tactccggtg ggcgcaacat gaccgtggtg cgcatcgata aagtctctta
    14401 tcgcagcaaa ccgattttcg aatcactcta tctcggtatg ccgtggaccg aaatcgacta
    14461 cctatgacta ggtaatttat tcaagataac cgggaagaga ttaaaaaaga cgggaataga
    14521 ttggaatcaa caagttagga actcaaggtt ttcaagataa agcgagtcca aaaatgttaa
    14581 acggttcgat atcaataaaa cattcaagat aactcacagg ttcttacaat agcctttaaa
    14641 gaccgttcaa aaacgattca ggtgtttatt atagcgctga tgggtgcaag cgccaaaacc
    14701 aatgtaattt tagcacagct aggcacttgc actgggttat gcgctggctg ctttctgagg
    14761 tagatttaag tgatcgccct tagcttcgga caacgcgtaa atctctctaa ccttttcgtc
    14821 tggcaaatta ccaaggctta acgctatggc tctacgaact ccaactagag cgtgcaactc
    14881 ttggatgtgc tcatccagaa gaatgagtaa ttgctccgct ccttttctcc caagaacatc
    14941 agctacttgt tgtttttctt gtggtttaag aaatgacaag aacattagaa tttgagcatc
    15001 aacattatcc gcattctctt ttttgggttc tttggaatct tttagttcaa catcaccctc
    15061 tcccgtcatg agccattcca aggtaacatt ttcgctttct gctatctttt ttaactgata
    15121 cgggcgtggt atcggtgcgt aatcccgcct gttgtaataa ttttttaaag tgccttcatt
    15181 gatcccccat gcccgagcag ccgcatttct tgaagggtat cgttctacta acctaaagat
    15241 tctttcgata atcggttctt ttccatcggc aggaaaagaa ccttggggtg cttttctcat
    15301 tcttggttcc tttctatcta attcattgat aaataataaa attaaaaaat aaaccataaa
    15361 aacaccaaag aaccctttgc agggaatctt tggtatctta atctttatcc atacggatat
    15421 ccgaccggat atccgcgcgg gtaattttgt aggttaaagg aaagatgaga caaagagaag
    15481 atagtgaccg cgattggcct tcgctgaaga ttcagctggc gctcaaagat aacgggtggt
    15541 cttccttccg ggagctttca ctccatgctg gttatgctca ggattcactg aaagcggtag
    15601 ttcgcacacc aaacaaaaaa tacgaacgcg taatttctga cgctataggt gtaccgccag
    15661 aaatcatctg gcccagccgg tatcgtaacg atggatttat caggaaggcg tcgtgatatg
    15721 cagtcgtacg ttaccgtaaa tgacctgctc ggcgtaccag gaatgccagc caccacaaaa
    15781 ggcatccgac aggcgttaca gcgtttttct cgtgatcttg gtgacgttag tcgccgtcgg
    15841 gaaggcacaa aagccattga gtaccacatt gattgtctgc ctgaaataac acgaaaggca
    15901 ctgcgtgaac gctatgtgga acagctggtg gctacagaga acaatgtttc tgaagtgaaa
    15961 gctgttacca gaaaaacacg caatcctgac gctgtccagg caatcgaagc atatcgcggt
    16021 tctccacaac tgatggaaga acgcctgaat gcgctgaccg aaaaccagcg ctgggtatct
    16081 gaagcaagag ctgcgctggt ggtggaagtg ctgaagctgg aaagcgccgg taaccccggg
    16141 cgactgaaag ccattaactt tcttgttgaa aaagcccgta aaggtgagct gccggagcgc
    16201 ctgcaacagg ccgcagttaa cgccaatgca aaacgtggcg ctaatcgtac tatcagccgc
    16261 gatccacttt accagtgggt tctgaaatac aaccagtcgc agaacgccgc cgaacggctg
    16321 ctgttactgg caccgggaaa acgcgacgaa ataaaaccgg aagaaatcag ctggctgcct
    16381 gaatttctgg cgcagtatcg ccaggtgaac ggcagaccca tgtcagaagc ctacgaggat
    16441 tttgtcgcag aatggcagcg tcgccatgcg gacgaaccgt acatgctgga ggtgatgcct
    16501 tcctatgatg ttgtccggta tgccatgaaa aaactgccgg aagtggtgaa acaaaaaggg
    16561 cgcgttaccg gcagtgaata ccgtcagctt gaaggattca cccgccgcga ctggacagct
    16621 atgccggtga attatgtctg gattggtgac ggtcacggta tgaagctgaa atgtgcgcat
    16681 ccgatccacg ggcgtccatt ctcaccggaa gtcacgtttg tgatcgatgg tggaacacgg
    16741 tttgttgttg gctggagtct tgaccttgcc gaaaacgtgt tcgccgttgc cggtgcgata
    16801 cagcacggca ttcgcaacca cggaaaaccg ttcctgtatt actcggataa cggttccggt
    16861 gaaacggctg acatgctgga caaagaggtc gtgggtattc tgccacgcct ggggattaaa
    16921 caccctaccg gtattgccgg taatccacag ggacgcggca ttattgaacg cctgaaccgg
    16981 acactgccga tgcgtatagc gaggaggtat cgcacctatt ttggcaaggg tgctgaccgg
    17041 gaatcgttgc gtgtacttaa ccgtgatctg cgttcagcgt ttaacgccct gcaacaggac
    17101 aagcctctga acgaccgcca gaaagcggca atgcgcgagc tgccatcgtg ggcggagctt
    17161 attgaggcta ttcgtgaagg cgtggaatgg tacaacaacc gcccgcattc tgagctgccg
    17221 atgaaaccga acggacggca ttacagcccg acagagttca gaaaaaaacg ccaggcagaa
    17281 gaagacaccg aaattgagtg gctgagtgat cttgagctgc gcgacatgtt ccgcccaatg
    17341 gtggaacgac cggtaagacg ctgcgaaatt cagtggctga acaatattta ctacgcgccg
    17401 gagttgcgtg acgaacacgg tcgcaaagtg cttatcagtt atgacattca tgacgccgag
    17461 cgaattacgg tacgtcgcaa ggacggcagc tttatctgtg aggcgatatg gaacggcaat
    17521 aaacgcgcag cctttgctgt cagtgctgaa tatcacaaac aacagcagcg tattaaagga
    17581 atgcgcaaac gcgctgagga aaaaattcgt gatgctgaag atgaaggtat tcagattctg
    17641 gaacacaaac aggctgaacc ctggctgagt aatgtttatc gcccggttgg taatgtggtg
    17701 gcagttcagc aaccggaata tgaagaagag catgacgaag aatttgaacg cgatttccgg
    17761 ctggggatgc aaaaactttt tgccatgcag gaagaagatg atccgctggc ctgaataaaa
    17821 aaacagtccg cgggaacgga ctgttaaata aaggaaacaa agctgatttg aaataaccga
    17881 ggaaatacta tgactgatat tgatgatgtt tgaggtgtac tggcaatagc ggacactacc
    17941 a
//
LOCUS       DAZPAB010000073        17884 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZPAB010000073 DAZPAB010000000
VERSION     DAZPAB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17884
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.073"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(142..282)
                     /gene="yjjY"
                     /locus_tag="ACHUPG_002153"
     CDS             complement(142..282)
                     /gene="yjjY"
                     /locus_tag="ACHUPG_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR0861764.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            378..1094
                     /gene="arcA"
                     /locus_tag="ACHUPG_002154"
     CDS             378..1094
                     /gene="arcA"
                     /locus_tag="ACHUPG_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR0861765.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1153..2505)
                     /gene="creD"
                     /locus_tag="ACHUPG_002155"
     CDS             complement(1153..2505)
                     /gene="creD"
                     /locus_tag="ACHUPG_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR0861766.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(2563..3987)
                     /gene="creC"
                     /locus_tag="ACHUPG_002156"
     CDS             complement(2563..3987)
                     /gene="creC"
                     /locus_tag="ACHUPG_002156"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR0861767.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(3987..4676)
                     /gene="creB"
                     /locus_tag="ACHUPG_002157"
     CDS             complement(3987..4676)
                     /gene="creB"
                     /locus_tag="ACHUPG_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR0861768.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4689..5162)
                     /gene="creA"
                     /locus_tag="ACHUPG_002158"
     CDS             complement(4689..5162)
                     /gene="creA"
                     /locus_tag="ACHUPG_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR0861769.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5373..6242
                     /gene="robA"
                     /locus_tag="ACHUPG_002159"
     CDS             5373..6242
                     /gene="robA"
                     /locus_tag="ACHUPG_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR0861770.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6239..6886)
                     /gene="gpmB"
                     /locus_tag="ACHUPG_002160"
     CDS             complement(6239..6886)
                     /gene="gpmB"
                     /locus_tag="ACHUPG_002160"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR0861771.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6938..7458
                     /gene="yjjX"
                     /locus_tag="ACHUPG_002161"
                     /pseudo
     CDS             6938..7458
                     /gene="yjjX"
                     /locus_tag="ACHUPG_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(7543..7869)
                     /gene="trpR"
                     /locus_tag="ACHUPG_002162"
     CDS             complement(7543..7869)
                     /gene="trpR"
                     /locus_tag="ACHUPG_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR0861772.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(7959..9896)
                     /gene="sltY"
                     /locus_tag="ACHUPG_002163"
     CDS             complement(7959..9896)
                     /gene="sltY"
                     /locus_tag="ACHUPG_002163"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR0861773.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10107..11774
                     /gene="ettA"
                     /locus_tag="ACHUPG_002164"
     CDS             10107..11774
                     /gene="ettA"
                     /locus_tag="ACHUPG_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR0861774.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(11881..12048)
                     /gene="nadS"
                     /locus_tag="ACHUPG_002165"
     CDS             complement(11881..12048)
                     /gene="nadS"
                     /locus_tag="ACHUPG_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFR0861775.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(12082..13314)
                     /gene="nadR"
                     /locus_tag="ACHUPG_002166"
     CDS             complement(12082..13314)
                     /gene="nadR"
                     /locus_tag="ACHUPG_002166"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR0861776.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(13335..14717)
                     /gene="radA"
                     /locus_tag="ACHUPG_002167"
     CDS             complement(13335..14717)
                     /gene="radA"
                     /locus_tag="ACHUPG_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR0861777.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14766..15734)
                     /gene="serB"
                     /locus_tag="ACHUPG_002168"
     CDS             complement(14766..15734)
                     /gene="serB"
                     /locus_tag="ACHUPG_002168"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR0861778.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            15840..16484
                     /locus_tag="ACHUPG_002169"
     CDS             15840..16484
                     /locus_tag="ACHUPG_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR0861779.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16512..17527
                     /gene="lplA"
                     /locus_tag="ACHUPG_002170"
                     /pseudo
     CDS             16512..17527
                     /gene="lplA"
                     /locus_tag="ACHUPG_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            17559..17822
                     /locus_tag="ACHUPG_002171"
     CDS             17559..17822
                     /locus_tag="ACHUPG_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0861780.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
BASE COUNT         4271 a         4977 c         4500 g         4136 t
ORIGIN      
        1 actagttgga ttattaaaat aatgtgacga aagctagcat ttagatacga tgatttcatc
       61 aaactgttaa cgtgctacaa ttgaacttga tatatgtcaa cgaagcgtag ttttattggg
      121 tgtccggccc ctcttagcct gttatgttgc tgttaaaatg gttaggatga cagccgtttt
      181 tgacactgtc gggtcctgag ggaaagtacc cacgaccaag ctaatgatgt tgttgacgtt
      241 gatggaaagt gcatcaagaa cgcaattacg tactttagtc atgttacgcc gatcatgtta
      301 atttgcagca tgcatcaggc aggtcaggga cttttgtact tcctgtttcg atttagttgg
      361 caatttaggt agcaaacatg cagaccccgc acattcttat cgttgaagac gagttggtaa
      421 cacgcaacac gttgaaaagt attttcgaag cggaaggcta tgatgttttc gaagcgacag
      481 atggcgcgga aatgcatcag atcctctctg aatatgacat caacctggtg atcatggata
      541 tcaatctgcc gggtaagaac ggtcttctgt tagcgcgtga actgcgcgag caggcgaatg
      601 ttgcgttgat gttcctgact ggccgtgaca acgaagtcga taaaattctc ggcctcgaaa
      661 tcggtgcaga tgactacatc accaaaccgt tcaacccgcg tgaactgacg attcgtgcac
      721 gcaacctgct gtcccgtacc atgaatctgg gtactgtcag cgaagaacgt cgtagcgttg
      781 aaagctacaa gttcaatggt tgggaactgg acatcaacag ccgttcgttg atcggccctg
      841 atggcgagca gtacaagctg ccgcgcagcg agttccgcgc catgcttcac ttctgtgaaa
      901 acccaggcaa aattcagtct cgtgctgaac tgctgaagaa aatgaccggc cgtgagctga
      961 aaccgcacga ccgtactgta gacgtgacga tccgccgtat tcgtaaacat ttcgaatcta
     1021 cgccggatac gccggaaatc atcgccacca ttcacggtga aggttatcgc ttctgcggtg
     1081 atctggaaga ttaatcggct ttaccaccgt caaaaaaacg gcgcttttta gcgccgtttt
     1141 tatttttcaa ccttatttcc agatacgtaa ttcatcgtcc gttgtaactt ctttactggc
     1201 tttcattttc ggcagtgaaa acgcatacca gtctatatta cgagtcacaa acatcatgcc
     1261 ggccagcgcc accaccagca cactggttcc caacaacagc gcgctatcgg cagagttgag
     1321 cagtccccac atcactccat ccagcaacaa cagcgcgagg gtaaacaaca tgctattgcg
     1381 ccagcctttc aataccgcct gcaaataaat accgttcatt aacgccccaa tcagactggc
     1441 gattatccat gccacggtaa aaccgatatg ttcagaaagc gccagcaaga gcaaataaaa
     1501 catcaccaat gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt gcgcggtgag
     1561 cgtttcaaaa acaaagaacg ccataaaagt cagtgcaatc agcagaatgg cgtacttagt
     1621 cgcccggtcc gttaattggt attgatcggc tggcgtcgtc actgcgacgc taaacgcggg
     1681 gaagttttcc cagccggtat cattgcctga agcaaaacgc tcaccgagat tattagcaaa
     1741 ccagctgctt tgccattgcg cctgaaaacc tgactcgcta gcttcccgtt tggctggtag
     1801 aaaatcacct aaaaaactgg gatgcggcca gttgctggtt aaggtcattt cgctattacg
     1861 cccggcaggc accacagaaa gatcgccggt accgcttaaa ttcagtgcca tattcagctg
     1921 caggttctgc ttccgccagt ccccttcggg taaagggata tgcacgccct gcccgccttg
     1981 ctctaacccg gtaccgggtt caatggtcag tgccgttccg ttaacttcag gcgctttcac
     2041 cacaccaata ccacgcgcat ccccgacgct aatcacaata aagggcttgc ctaagatgat
     2101 atttggcgcg ttgagctcgc taagacgcga aacatcgaaa tcggctttta gcgttaaatc
     2161 actgtgccag acctgaccgg tataaatccc tatcttgcgt tcttccacgt tctgattgcc
     2221 atcaaccatc aatgactcag gtaaccaaaa atggataaaa cttcgtttcc gctccacggt
     2281 tttatcctct tcctgcaccg tataaagctc ggtcacagga atagcgataa gcggcccaac
     2341 gagtttttgc ggcccgctgg tactttggcg aatcgcatct tccacatcgc tacggtaatc
     2401 ggcacgttcg acaatcactt gccgaatcag cattatcgga atcaacaaca gcaatactgc
     2461 accaaacaag gtggtcattt tccagaacag gggggatttc aacatagtct tctcctttgc
     2521 aatggcagca ggatacgaag gctatgtggg aagaatttga agctatgtga agtgacggtg
     2581 aagtcgtagc gaggccagca cgccaccttc ctgcacgttg cgcagcgtga cttcgccgtt
     2641 aaacaaacgg gcgacctcac tgacgaacgc caaccccaga ccgctgcttt tttgcccatt
     2701 tgcacgcggc aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt caggaatacc
     2761 actaccggta tccagcacct taagcgtgac gtgttcctga tccacttcgg cgcttagcgt
     2821 tatgcaaccg ctctcgggag taaaatcgat ggcgttatcc agtaaattcc ccagcgcctg
     2881 atccagtaac gccggttcag cagcaacatt aacctcggtg ggcataacat gcaaagtgat
     2941 gtttttttct gccaactgca cggtgcgcgc ttcgctgacg cggcgaaata atgccgccac
     3001 atcaacagca gtcagaacga cttcctgacg attctccagt cttgcctggc gtagtaacgt
     3061 ttccaccagc gcctgcattc gcgcattttg tgtcagaatg ttgtcagtaa aacgagccac
     3121 tacttcaggc ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg ccgccagtgg
     3181 gctttttagc tcatgggtta acgcataaac atactgctca atatagtttt tcccttccag
     3241 cttcacgcgc atactttcca gcgcctgcgc gagtttacgc agctcgctac taccgagatc
     3301 gggaagagga acgggcttat tgtcagtgac ggaatcagca tagcgagtga gccgggcaat
     3361 ggagcggttg atccaccaaa ccatgcctgc gccaatcacc agtgcaatcc ccaacaaaat
     3421 ggcgctggcc cataaaattc gccgctcgct gcgtttaatg accggagcca tcgccgcgtt
     3481 cggtttgcct acgctcaaaa cgccaataag ccgcgagccg cccataatcg gcgcggcaac
     3541 atacatcacc gaactttcgg gatcggcagg attttgcaac gtgctgcgcg caccatactg
     3601 accacgcaac gttagccaga cgtcattcca gcgcgaataa tcctgtccaa cggctttatt
     3661 tgccgaatcg aacaatactt tgccatgcgc atcggtcata tagacatgat attcattgcg
     3721 cactttgtta atgccaccaa tattggcgcg aaacgggcga tgttgtagct gattaaacgc
     3781 ctgcgccagt tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg ccagctccgc
     3841 aagtaacgtt gccgtgtcga tcaacgtccc ctccgttgct cttcgcacgc ccggtttaac
     3901 ttctttgaca aaaatagcca gtacgaacca ggccgccacc gccaccagta aaaaatagcc
     3961 cagcagcaac cgcatgccga tacgcattac aagcccctca ggctatatcc catgccgcga
     4021 tgagtattaa tcggtgaaag atcggggttg atggcgcgca gcttggcacg cagcgtttta
     4081 atgtggatat cgacggtgcg atcgtaggtg tcctgcgcat cttcccacac gctatccatt
     4141 agttgctggc gggaccagac gcggcctggc gacttgagta acgtcttcag caataaaaac
     4201 tcataccgcg tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc gggttcattc
     4261 aattcaaaat gaccaatacg gatgacggga gacggcgtcg agaacttctt cacccgacgc
     4321 agtaaggtgc gcaccctggc gcacacttcg cggggtgaaa acggtttagc cacatagtcg
     4381 tcagcgccaa tttccagccc aagcaggcga tcgacctctt cacttcgggc cgtcaggaac
     4441 agtacaggta acgccggata gagcgccagt aactggcggc acaattcaaa gccactgata
     4501 tcaggcaaac caacgtcgag aatcatgacg tcgggaacct gctggcgagc tttatccagc
     4561 accggcaaac ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa catgtagacc
     4621 agcgtgtcgg ctatcccttg ctcatcttcc actaaccaga ccgtttcccg ttgcataaat
     4681 cgcctctgtt attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt tttcggcgaa
     4741 ccttctacaa ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt ggcgtcataa
     4801 aagcgcacga cctgtaacga cttaaagacc agggacgtgc gttttttgaa tactacctcg
     4861 ccctgagctt tgccgttttt aatacgatcc gacagttcaa tcggcccgac ttgctgacaa
     4921 gaaatggccg catcggaggt atcttctgcc aggcccaatc cccctttaat accaccggtt
     4981 ttcgcccggc tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa ggcttccaca
     5041 acaattttgt gatccgggcc gatcatttta aatacggtgt cgaccgaacc aatctcttct
     5101 gcatgagcca atggccccag cattatcagg cttaaagaca ggatcaaatg cttgtatttc
     5161 atattgttac catttttatc tattacacga gcaattagtt cgtcacggta aataacatat
     5221 tgattaagaa caaaatctca attcttttat ttccgtcaag ccctaaaaca tactctacta
     5281 agaaaaaaac actgaatgct aaaacagcaa aaaatgctat tatccaatta cctgatgtca
     5341 ggtgctcgtt gttgaaagga tgaggatatt ttatggatca ggccggcatt attcgcgacc
     5401 ttttaatctg gctggaaggt catctggatc agcccctgtc gctcgacaat gtagcggcga
     5461 aagcaggtta ttccaagtgg cacttacaga gaatgtttaa agatgtcact ggccatgcta
     5521 ttggcgcgta tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta cgcctgactg
     5581 cgcgtccgat tctggacatc gcgctgcaat accgcttcga ctctcaacag acatttaccc
     5641 gcgcattcaa gaagcagttt gcccagactc ctgcacttta ccgccgttct cctgaatgga
     5701 gcgcctttgg tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca gagcacaaat
     5761 ttgtcaccct ggaagatacg ccgctgattg gtgttaccca gagctactcc tgttcgctgg
     5821 agcaaatctc tgatttccgc catgaaatgc gttatcagtt ctggcacgat tttctcggca
     5881 acgcgccgac cattccgccg gtgctctacg gcctgaatga aacgcgtccg agtcaggata
     5941 aagacgacga acaagaggta ttctatacca ccgcgttagc ccaggatcag gcagatggct
     6001 atgtactgac ggggcatccg gtgatgctgc agggcggcga atatgtgatg tttacctatg
     6061 aaggtctggg aaccggcgtg caggagttta tcctgacggt atacggaacg tgcatgccaa
     6121 tgctcaatct gacgcgccgt aaaggtcagg atattgagcg atactacccg gcagaagacg
     6181 ccaaagcggg agatcgccca attaatctgc gctgtgaact gctgattcct atccgccgtt
     6241 aacgctgcag ctcatctaat gcaggggcgt ccagatgcga gatgtcccct gcggtttcaa
     6301 cgacccagcc agacgccagc cacaggcttt cctgataatc cacgcgcgaa atagaacagt
     6361 tacgcagacg taagcggcgc tctgcccatg ctggtaatcc gagaatcgta ctcaccagac
     6421 atcccagtgc aataccatgg ctcaccagca gcggtcggct tccctgcggt aagtcccggc
     6481 aagattccag tgccgcattg acgcgatcgc tgagctcctg cattgactcg ccttcaggaa
     6541 tacgcccgtc aacggtgcca ttgaccagct gccgacgcca gttctcttct tcttcggtca
     6601 gagaatcgat atgtcttttt tccagcacac ccatgtttaa ttcacgcagg cgagaatcaa
     6661 agatgatgtc acagccgcag gcctgggcga tgatttccgc cgtacgccgg gtgcgtccta
     6721 aatcgctact gataatatgc gtaatgccaa gctctttggc acgggttgcc acctgcatcg
     6781 cctgttgctc acctttggcg gtcagcgggc tgtcagactg gccctgaata cgtcgctcgg
     6841 cgttccactg cgtttcaccg tggcggacta ggtatacctg taacatgctt tttttccgta
     6901 tactgctgta acgttaattt tctgagtaat gctgattatg caccaagttg tctgtgcgac
     6961 caccaatccc gctaaaattc aggccattct gcaggcattt cacgagatct tcggcgaagg
     7021 atcctgccat attgcatccg ttgccgtcga gagcggtgta ccggaacaac cctttggcag
     7081 tgaggaaacg cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt tacttccaga
     7141 ggctgatttt tgggtggcga ttgaagctgg cattgatggc gacagcactt tcagttgggt
     7201 ggtgattgaa aacgccagcc agcgcggcga ggcgcgttcg gcaactttgc cgttacctgc
     7261 ggtgattctg gaaaaagttc gtgaaggcga ggcgcttggt cccgtgagtc gcgttatacc
     7321 ggtattgatg agattggtcg taaggaaggg gcgatcggcg tgtttaccgc cgggaaactc
     7381 actcgcgcca gcgtctatca ccaggcggtg atcctggcgc tgagtccgtt tcacaatgcc
     7441 gtgtatcagg cattgtagga cggataaggc gttcacgccg catccgacat tcggtgcacg
     7501 atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aatcaatcgc ttttcagcaa
     7561 cacctcttcc agccactgac gcagctccac gggcgcggct ttcaggctgt tagatccacg
     7621 cgtaatcgtc gcgatgcccg cgccgagttc attttttaac tcacgctgac tcatttcgcc
     7681 acgcaacagc tcttcgacaa tacgcacgcg agtccccaac gcttcgcgct catctggcgt
     7741 cagcatcagg tttaacaacg gtaaatggag atcgttttgg taggcattct taagcaggtc
     7801 gacaaaacgt aaccactcct ggtgacgctg ttctgccatc gctgctgaat agggtgattg
     7861 ttgggccata atatgtcgcc attgttagcg ggggaagcaa aatgactccc ccggttgtac
     7921 tcgctaaaga gtacgatagc atatcataaa cgtgcggatc agtaacgacg tccccattcc
     7981 gtggcgctca tcaacgtcgg tttatccccc atgaaatagc ggtagtaagc atcataagcc
     8041 agcacgttct tcacataacc gcgcgtctcg gagaacggaa tactctcgac aaatgccaca
     8101 gcgtcgatac gcccggcgct gttgccaagc caggttcgca cccgtccagg tccggcgtta
     8161 taagctgctg aggagaaaat acggttattg ccaaactgct gataaacata ttgcaggtaa
     8221 ctggtgccaa tgttgatgtt cgtttccgga tccagcaatt gcccagggct gctataacca
     8281 ggaatagaga acatcttcac cgtatgggtc gctgtaccag gcataatctg catcaggccg
     8341 ctggccccta ccggtgattt cactttcgga ttccaggcgc tctcctgacg ggcaatcgcc
     8401 atcgcatagc tttgcgggat ctctttccca ctggtgtaac gtttgaaaag atcgttgtaa
     8461 gccagcggga atcgctcttc cagatgatcc caaagcttcc cggcgatcgt tgcctgaacg
     8521 ctaagatccc accattggtt attgaaagca taccgagcca gttgagcctg ctctgttttt
     8581 gacttgctct tcaccagatt ggcccactcg ctacgcgcgg tattatcgag attccagtac
     8641 atcaactcgc gcacgcgcgc catctccggc ccctgagtca gggtgctgtc aacattctgc
     8701 ggcgctttat caatcttcag ctcatactct tcaccgatgc gttgtgctgc aaccatcggg
     8761 tagaaaccac gctgttgtat gagttgatgc aaaatctctt tcgcttcagc ttcacgtccg
     8821 cgttccagca ataaatccgc ctgccagtaa cgccattcat ctttctcttt tgcttccatc
     8881 ggcagacgcg ccagccaggt attcaggccg cgacgatcgc cggtgccaag cgccattcgc
     8941 acacggcgtt caatcaacga cgtggattgc gagcgcataa tggcgtcatc gcgccatttc
     9001 gcctgctcat cggtgacatc attgcccatc aaacgccagg cgacgatatc gcgcagctcc
     9061 tgaatctgat cttcattaag ctgctgcgcc tgggcaagcg acgggatcat cagccgtgca
     9121 ttttcggcat cctgccgcgc cacactggca aacgccaccg ccgccatttg acgggtaaaa
     9181 tcggtcgcgc cggttgtacg cgcgaaggtc agtaccgtat tagggttgtt cgccagtgaa
     9241 atgattgccg aggcgatagt ctggtaatcg gcaggcatct gccctgccag cactgttacc
     9301 aggcctgtgt tacccgcttt catcgccaga cggatacgct ctaaatacgc cagcggatct
     9361 tgtttacctg acgcacgcca gacgctaaat aacttgtcac aggcgttagg ctggctcttg
     9421 ccggttagcc acagctcttt cgccccttgc caggcttctt cactctgccc ggtgttccat
     9481 ttcgcatagt agtaattaca ttgcgcttcg gtagttccgg gcttttccgg gctaaaggct
     9541 aacaagccac gccagtcttc acgccgcgcc agttcattga cgaaacgaga ttgcagcgtg
     9601 cgagcgggag gaagcgtggg gttagcgcga acaaagttag tgaccgtcac cgccggttga
     9661 ttcatcaggt catcggtgat ctggcggtat tccaggtagg gataaagcgg ataatccttc
     9721 agtccaggca tcatttgttc gaccacatcc atctgtcgat tatcccaggc ctgcttgatt
     9781 tgcgcgtaac ggctacgctg ctcatccagt gagtcggctc gcgccacgct gctgaccgtc
     9841 agcagacaga caccggcagc caacagccgc caggtaactt gtttggcttt ttccacaagc
     9901 gcatcctcta agtgtaaata catcaatgca gcgtcatgcc gcgtaataga tttatgctaa
     9961 ccagtcattg ccgtttacgc cacgttacgg acactttttt acttttactg cgaaggtgat
    10021 cgaccaactg ggattaggcg gggaaacagg ctacacttcg cctttgaaaa ctcatcatca
    10081 cgataaacat agaggcgaag tccaacgtgg ctcaattcgt ttataccatg catcgtgtcg
    10141 gcaaagttgt tccgccgaaa cgtcatattt tgaaaaacat ctctctgagt ttcttccctg
    10201 gggcaaaaat tggtgtcctg ggtctgaacg gcgcgggtaa gtccaccctg ctgcgcatta
    10261 tggcgggcat tgataaagac atcgaaggtg aagcgcgtcc gcagccagac atcaagattg
    10321 gttatctgcc gcaggaaccg cagctgaacc cggaacacac cgtgcgtgag tccattgaag
    10381 aagcggtttc agaagtggtt aacgccctga aacgcctgga tgaagtgtat gcgctgtacg
    10441 ccgatccgga tgccgatttt gacaagctgg ccgctgaaca aggccgtctg gaagagatca
    10501 ttcaggctca cgacggtcat aacctgaacg tacagctgga gcgtgcggcg gatgcgctgc
    10561 gtctgccaga ttgggacgcg aaaatcgcta acctctccgg tggtgagcgt cgtcgcgtgg
    10621 cgttgtgccg cctgctgctg gaaaaaccag acatgctgct gctcgacgaa ccgaccaacc
    10681 acctggatgc cgaatccgtg gcgtggctgg aacgcttcct gcacgacttc gaaggcaccg
    10741 tggtggcgat tactcacgac cgttacttcc tcgataacgt tgcaggctgg atcctcgaac
    10801 tggaccgcgg tgaaggaatt ccgtgggaag gtaactactc ctcctggctg gagcagaaag
    10861 atcagcgtct ggcgcaggaa gcttcacaag aagcggcgcg tcgtaagtcg atcgagaaag
    10921 agctggagtg ggtacgtcag ggaactaaag gccgccagtc gaaaggtaaa gcacgtctgg
    10981 cacgctttga agagctgaac agcaccgaat accagaaacg taacgaaacc aacgaactgt
    11041 ttattccacc tggaccgcgt ctgggcgata aagtgctgga agtcagcaac ctgcgtaaat
    11101 cctacggcga tcgcctgctg attgatgacc tgagcttctc gatcccgaaa ggggcgatcg
    11161 tcgggatcat cggtccgaac ggcgcgggta aatcgaccct gttccgtatg atctccggtc
    11221 aggaacagcc ggacagcggc accatcactt tgggtgaaac ggtgaaactg gcatcggttg
    11281 atcagttccg tgactcaatg gataacagca aaaccgtttg ggaagaagtt tccggcgggc
    11341 tggatattat gaagatcggc aacaccgaga tgccaagccg cgcctacgtt ggccgcttta
    11401 actttaaagg ggttgatcag ggtaaacgtg ttggcgaact ttccggtggt gagcgcggtc
    11461 gtctgcatct ggcgaagctg ctgcaggttg gcggcaacat gctgctgctc gacgaaccaa
    11521 ccaacgacct ggatatcgaa accctgcgcg cgctggaaaa cgccctgctg gagttcccgg
    11581 gctgtgcgat ggttatctcg cacgaccgtt ggttcctcga ccgtatcgcc acgcacatcc
    11641 tggactacca ggatgaaggt aaagttgagt tcttcgaagg taactttact gagtacgaag
    11701 agtacaaaaa acgcacgctg ggcgcagacg cgctggagcc gaagcgtatc aagtacaagc
    11761 gtattgcgaa gtaatgcgta aaatgccgga tgcggcgcga acgccttatc cggcctacaa
    11821 aaccagcaat ttcaatacgt tgtaaaactg taggcctgat aagacgcggt aagcgtcgca
    11881 tcaggcattg agcaccgaat gccggatgcg gcgtgaacac cttttccggt ctacaacagc
    11941 cttagcatcc tcaatttcgt ggcgggtcac ccgtgcaggt tttttcaaac cttgtttaat
    12001 ctcgacagcc tcttccagag aggttttcaa ctcatcaaaa aaactcattt tatacctcgc
    12061 ctttgagcag tttcgtcacg gttatccttg ctcccccatc atctcccgca ccagctccac
    12121 gcagcgcagg aaacggctgt cgtaatcgtc ctcttcaacc cgcacgaatt cgatattgtt
    12181 ctcttccagc atctccacca gcaagttctg gaactctttg cgatccaccg aactgccgag
    12241 gctgcgtaaa ccatccgcca cccacggcgt gttgttctcc agcaggatca ccagatcgaa
    12301 acggtattca tcaatcagcg cctgcacgaa cggatgctca cgcccttcgt actttttgca
    12361 gaacgcctgg gtagtgacaa aatcggtgtc aataaacgcc actttattgg cgtatttcac
    12421 tgcaaaatca atgtattgtg cgtgacccaa cgcgatttta tcgtagtcgg aatactgcaa
    12481 tgcgatttca tcaccgccga ggtgtgaaaa gacataatcg cggccatatt cccacgcact
    12541 ggtggtgttg aagatattgg caagtttgtt taccagggtg gatttaccgc tcgactcgcc
    12601 gccaaggatc gccacggtac gcacaaaaaa cggcttcact tcggtaggaa tatattccca
    12661 gtagcggaac gggttttcgc ggatctgcgc accgctgata ctcataaagg tacgtttcgg
    12721 atcgaccagc accgtctcga tccccagatg ttccatatac tgtggcgcat cggcttcttc
    12781 cgaggtgtag atcaggtccg gctggatccc cttttcagcc ataaactttt tgatgccgtt
    12841 gctccacaca tcccagccat gcggatacgg ctccatgccc tcttcgttga aagcatgaat
    12901 acggatattt ttctgatatt taaaggtttg caatagccag cgcagacgat ccggcaccgt
    12961 gggctgctgc gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat cgtcaaaacc
    13021 cataatgata tgcagctcgt caacctggct acaggcgcgc tggataaggt agatatgtcc
    13081 ggtatgcagc gggtagaact taccgaatac gacaccaatg gttttcttct gccggggaaa
    13141 ttcaagcccc aaaaaacggt gcaatgcctc cagcttttgc gcgctggggc ttttaatttt
    13201 ggcattcagt aactggctta aatacccttt ggtcataccg ctggcatcag ctacctgctg
    13261 tagcgtgcag ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg acatatctcc
    13321 ctccgtatct ctcattataa gtcgtcgaac acgctaagcg cgtcggagag ttttttaacg
    13381 ccaaaaatct gcatcccttc cggcgctttt ttcggtacgt tagccgccgg aacaatcgcc
    13441 cggcgaaaac cgtgtttcgc cgcttcagaa atccgctcct ggccactggg caccgggcgg
    13501 atctcccctg ccagcccgac ttcaccaaac accaccagat cctgtggcag cggtctgtca
    13561 cgcaggctgg aaaccatcgc cagcagtaac gctaagtcgg cgctggtttc ggttaccttc
    13621 acgccgccga ccacgttcac aaacacatcc tgatcggcca tttgcagacc accgtgacgg
    13681 tgcaagacag ccagcaggat tgccagacgg ttttgttcca gccccactgc cacgcggcgc
    13741 ggattcgcca ttatcgagtg atcgaccagc gcctgaatct ccaccagcaa cggacgcgtt
    13801 ccttcccaca ccaccatcac ggagctgccg gaggtcactt catctccgcg acttaagaaa
    13861 attgccgaag ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa gacgcccagc
    13921 tcattcaccg cgccgaagcg gtttttatgg ctgcgtaagg tgcgaaaacg ggagtcggca
    13981 tcgccatcca gaagcaccga acagtcgata cagtgttcca gcactttcgg gccagccagc
    14041 gaaccatctt tggttacgtg tccaaccatg acaatcgcca caccgcgcgt tttggcgaaa
    14101 cgcgtcagat aagccgccgt ttcacgcacc tgcgccacgc taccaggcga tgattgtaca
    14161 tccgccatat gcatcacctg gatcgagtca attaccatca gcttcggttg ctcttcttcg
    14221 gcaatcaggc agatctgctc gatgctggtt tccgacaaca tattgagatt gtcagtcggc
    14281 aggccaaggc gatgagcgcg cattgccacc tgttgcagcg actcttcgcc ggtgacatac
    14341 agcgttttca tctgctgggc cagtttgcac agcgtttgca gcaacagcgt ggatttcccc
    14401 gcaccagggt taccgccaat cagaatggca cttcctggca ccacgccgcc tcctagtacg
    14461 cggtcgaact ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct gatatcggag
    14521 agtttctgga cttttgccac cccggcgcta ccggcatagc cgctgagacg ctcgttacgc
    14581 gccaccgttg gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc atgacaggca
    14641 ctgcactgcc cctgccagcg cggataatcg gccccgcatt cattacaaac aaaggcgcgt
    14701 tttggagctt ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg atgacgggca
    14761 ggcaattact tctgattcag gctgccagag aggatgcaga atacccccat caggtcagcg
    14821 tgacggatgg tgacttccgc cttttcattc acttttggct tggcatggta ggcaatcccc
    14881 agccctgctg ctttgatcat cggcaggtca ttagctccat cgccaatcgc cacggtctgc
    14941 gccagcggga tttcatactc ctgcgcgagg cgagtcagag ttttcgcttt gtactgcgcg
    15001 tctacgatgt cgccgatcac attgccggta aatttaccgt ccatgatctc cagttcattg
    15061 gctaccacgg ctgtcaggcg cagcttgtcg cgcaggtatt cagcaaagaa agtaaagccg
    15121 ccggaggcaa tcgccacttt ccagcccagc gtttccagct tgagcaccag ctgcgttaag
    15181 cctggcatca gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc agcgcctttc
    15241 agcgtcgcca cgcggctgcg caggctggcg gtaaaatcga gttcgccgcg catcgcccgt
    15301 tcggttacct ccgccaccat ctcgcccgtt ccggccagct tggcaatttc atcaatacat
    15361 tcaatctgga tggcggtgga atccatgtcc atcaccagca aacccggcgt gcgcaggtgc
    15421 gggattttcc ccagcggggc gacatccagc tgcgcttcgt gggccaggcg tgtagcccgt
    15481 gcggtgagtg aacctgccag acgaatcacc tgataatctt ccacgcacca ggcggcaaca
    15541 atcaccatcg ccgcacccag tttgctctgg tattgggtca gacgctgttt atccagccca
    15601 cgaccataca gcagccagcc gctacgacct gcgtggtaat ccagtggcat cacttcatca
    15661 ccacttaatg aaagaggcag acccggccat aaagagacat cttcaggcag gtcgcaccag
    15721 gtaatgttag gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat aacgcatgag
    15781 gctaccttgt atccattgct tctggcaaca ttaagtctca aattttcaaa gggtggaaga
    15841 tggctcgcac aaaactgaaa ttccggctac atcgggcagt gattgtcctg ttctgtcttg
    15901 ccttgttagt ggcactgatg cagggggcgt catggtttag tcaaaaccac cagcgacagc
    15961 gtaatccaca gctggaagaa ctggcccgca ccctggcgcg tcaggtgacg ctgaacgttg
    16021 caccgctgat gcgtaccgac tcaccggatg aaaaacgcat tcaggcgatc ctcgatcagt
    16081 taacagatga aagccgtatc ctcgacgcgg gtgtgtatga cgaacaaggc gatcttatcg
    16141 cacgttctgg cgaaagcgtc gaagtgcgcg atcggctggc gctcgacggt aaaaaagcag
    16201 gcggctattt taaccagcag attgtcgagc caattgcggg taaaaacgga ccgctcggct
    16261 atctgcgcct gacactcgac acccatacgc tcgccaccga agcccaacag gtggataaca
    16321 ccactaacat tttacgcctg atgttgctgc tctcactggc aatcggcgta gtgctgaccc
    16381 gcacgctgct acagggtaaa cgcacccgct ggcagcaatc gcccttcctg ttaaccgcca
    16441 gcaaaccggt gccggaagag gaagaaagcg agaaaaaaga gtgacccatt actacaagaa
    16501 aggaaatcgt tatgtccaca ttacgcctgc tcatctctga ctcttacgac ccgtggttta
    16561 acctggcggt ggaagagtgt atttttcgcc aaatgcccgc cacgcagcgc gttctgtttc
    16621 tctggcgcaa tgccgacacg gtagtaattg gtcgcgcgca gaacccgtgg aaagagtgta
    16681 atacccggcg gatggaagaa gataacgtcc gcctggcgcg gcgcagtggc ggtggcggcg
    16741 cggtgttcca cgatctcggc aatacctgct ttacctttat ggctggcaag ccggagtacg
    16801 ataaaactat ctccacgtcg attgtgctca atgcgctgaa cgcgctcggc gtcagcgccg
    16861 aagcgtccgg acgtaacgat ctggtggtga aaaccgtcga aggcgaccgc aaagtctcag
    16921 gctcggccta tcgcgaaacc aaagatcgcg gcttccacca cggcaccttg ctactcaatg
    16981 ccgacctcag ccgcctggca aactatctca atccggataa aaagaaactg gcggcgaaag
    17041 gcattacttc agtgcgttcc cgcgtgacca acctcaccga gctgctgccg gggctcacca
    17101 tgagcaggtt tgcgaggcca taaccgaggc ctttttcgcc cattatggcg agcacgtgga
    17161 agcggaaatc atctccccgg acaaaacgcc agacttgcca aacttcgccg aaacctttgc
    17221 ccgtcagagt agctgagagt ggaacttcgg tcaggctccg gcattctcgc atctgctgga
    17281 tgaacgcttt agctggggcg gcgtggaact gcatttcgac gttgaaaaag gccatatcac
    17341 ccgcgcccag gtatttaccg acagcctcaa ccccgcgccg ctggaagccc tcgccgggcg
    17401 actgcaaggc tgcctgtacc gcgcggatat gctgcaacaa gagtgcgaag cgctgttggt
    17461 tgacttcccg gaccaggaaa aagagctacg ggagttgtcg acgtggatag cgggggcggt
    17521 aaggtaactc gctgattaaa atatccaagg aaggaaatat gatcgatctg gaaaaccagg
    17581 aacgggagat tatcaattta atgttcagcc agcgtatttc ctggctagcc gccgtgcgta
    17641 tcaggcataa attatcgctt gctgaagtaa gtaaaatgtt aggtatctcg attaattctc
    17701 taaaacagat cgaaaagacg gaacggctta gctcaaacat caaaagcaaa atggcagaaa
    17761 tttatggttg tccaccagag ttgctgatat gcccttcctg gatgacggct gaacataaat
    17821 gaatagattc gtgaatagat acttgaatgt tttttatttt ctatccatat gaaaaataat
    17881 agta
//
LOCUS       DAZPAB010000074        17677 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZPAB010000074 DAZPAB010000000
VERSION     DAZPAB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17677
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.074"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            73..756
                     /locus_tag="ACHUPG_002172"
     CDS             73..756
                     /locus_tag="ACHUPG_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR0861781.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            800..5462
                     /locus_tag="ACHUPG_002173"
                     /pseudo
     CDS             800..5462
                     /locus_tag="ACHUPG_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            5830..7485
                     /gene="lldP"
                     /locus_tag="ACHUPG_002174"
     CDS             5830..7485
                     /gene="lldP"
                     /locus_tag="ACHUPG_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR0861782.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7485..8261
                     /gene="lldR"
                     /locus_tag="ACHUPG_002175"
     CDS             7485..8261
                     /gene="lldR"
                     /locus_tag="ACHUPG_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR0861783.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8258..9448
                     /gene="lldD"
                     /locus_tag="ACHUPG_002176"
     CDS             8258..9448
                     /gene="lldD"
                     /locus_tag="ACHUPG_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR0861784.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9644..10117
                     /gene="trmL"
                     /locus_tag="ACHUPG_002177"
     CDS             9644..10117
                     /gene="trmL"
                     /locus_tag="ACHUPG_002177"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR0861785.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10170..10991)
                     /gene="cysE"
                     /locus_tag="ACHUPG_002178"
     CDS             complement(10170..10991)
                     /gene="cysE"
                     /locus_tag="ACHUPG_002178"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR0861786.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGCGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11071..12090)
                     /gene="gpsA"
                     /locus_tag="ACHUPG_002179"
     CDS             complement(11071..12090)
                     /gene="gpsA"
                     /locus_tag="ACHUPG_002179"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR0861787.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12090..12557)
                     /gene="secB"
                     /locus_tag="ACHUPG_002180"
     CDS             complement(12090..12557)
                     /gene="secB"
                     /locus_tag="ACHUPG_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR0861788.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12620..12871)
                     /gene="grxC"
                     /locus_tag="ACHUPG_002181"
     CDS             complement(12620..12871)
                     /gene="grxC"
                     /locus_tag="ACHUPG_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR0861789.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13013..13444)
                     /locus_tag="ACHUPG_002182"
     CDS             complement(13013..13444)
                     /locus_tag="ACHUPG_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR0861790.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13689..15233
                     /gene="gpmM"
                     /locus_tag="ACHUPG_002183"
                     /gene_synonym="pgmI"
     CDS             13689..15233
                     /gene="gpmM"
                     /locus_tag="ACHUPG_002183"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR0861791.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15267..16526
                     /gene="envC"
                     /locus_tag="ACHUPG_002184"
     CDS             15267..16526
                     /gene="envC"
                     /locus_tag="ACHUPG_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR0861792.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16530..17489
                     /locus_tag="ACHUPG_002185"
     CDS             16530..17489
                     /locus_tag="ACHUPG_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFR0861793.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17476..17677)
                     /locus_tag="ACHUPG_002186"
                     /pseudo
     CDS             complement(17476..17677)
                     /locus_tag="ACHUPG_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4410 a         4601 c         4636 g         4030 t
ORIGIN      
        1 atagctcacc aataatacaa tggacaactc taccggcatt gagcgctggt cggaggaaat
       61 aaggattttt acatgaactt gaagaagatt tttttcagtg ctgtcactgt ttcagttctc
      121 tgcgcattaa cgggatgtga ctacattgaa gaaggcaagc cagaaagtag tttactgaaa
      181 caacaagaag aacataataa taaaattgtg ctacttgaaa aacaacaagc acagctaaag
      241 agtcaactcg aaactattca aaaacaacaa actggcataa taaacagtac aaagacttta
      301 actcacgtaa ttaaatccgt taaagaccaa caaaatactt ttatttttac cgagtttaac
      361 ccagcaaaaa cgaagtattt catcctgaac aacggttcgg tggctttagc gggtcgagtg
      421 ttatccattg acgccacaga gaatggtagt gttattcata tctcactggt caacttatta
      481 agtacaccta tctcaaacat tggttttaat gcgacatggg gtggtgaaaa acctgtcgat
      541 gccaaagagt ttgcccggtg gcaacaatta cttttcaaca catcaatgaa atccacattg
      601 aaattattac caggtcaatg gcaagacatt aatttgaccc tgaagggtgt atcgcccaat
      661 aacctgggat atctgaagtt agccatcaac atggaaaata ttcagttcga caatcttccc
      721 tctgctgaaa atcggcagaa aagaagcaaa aaataaatgc agttagtacg cttataacaa
      781 ggaatagcga gcatcattaa tgaacaaaat atttaaagtt atctggaacc ctgcgacagg
      841 gaattatact gttaccagcg aaacggcaaa aagccgtggc aagaaatctg ggcgcagtaa
      901 gctgttaatt tctgcgctgg ttgcgggtgg aatgttgtcg tcgtttgggg cattggcgaa
      961 tgccgggaat gacaacggtc agggtgttga ttacggtagt ggatcagctg gcgacggctg
     1021 ggttgctata ggcaaagggg cgaaagcaaa tacttttatg aacaccagtg gttccagtac
     1081 tgctgtgggt tatgacgcta tagctgaagg ccaatatagc tctgccatcg ggtcaaaaac
     1141 ccatgcgatt ggtggtgcat caatggcctt tggggttagt gcaatatcag aaggcgatag
     1201 aagtatagca ctgggtgcct cttcgtattc attgggccaa tactcaatgg ccctcggccg
     1261 ttattcaaaa gcattgggta aattgtctat tgctatgggg gactcttcca aagcggaagg
     1321 agcaaacgcc attgccctgg ggcgtagcag tgtagctagc ggtactgaca gcctcgcatt
     1381 tggcagacaa tcacttgcca gcgcagcgaa cgctattgcg ataggtgctg agaccgaagc
     1441 cgctgaaaat gcaactgcta ttggcaataa tgcgaaggca aaagggacta atagcatggc
     1501 aatggggttc ggaagccttg ccgataaagt caatactatc gcattaggaa atggcagcca
     1561 ggctctggca gataatgcaa tcgccatagg ccagggcaac aaagctgatg gcgtggatgc
     1621 catcgctctg ggtaatggta gccagtcgag aggcttaaac accattgcct taggcacagc
     1681 cagtaatgca actggtgata agagtcttgc gcttggtagt aatagcagtg ccaacggtat
     1741 taactctgtc gcgctgggcg cagattccat tgcggattta gacaataccg tctctgtcgg
     1801 caatagttca ttaaaacgca agatcgttaa tgtgaaaaat ggcgcgatca agtctgacag
     1861 ttacgatgcc attaatggtt cacagcttta tgccattagc gactcggtag caaaaaaggc
     1921 ttggaggagg ggctgcagta gatgttgatg acggtactgt tacagcacca acctacaatt
     1981 taaaaaatgg tagcaaaaat aacgtagggg ctgcgctcgc tgtacttgat gaaaacaccc
     2041 tgcaatggga ccaaaccaaa ggcaaataca gcgctgctca tggtactagt agcccaactg
     2101 ccagcgtaat caccgatgtt gcggatggca cgatttcagc ctccagtaag gatgcggtta
     2161 acggttccca actgaaagct accaatgacg atgtcgaagc caacaccacc aatatcacca
     2221 acctgacgga ttccgttggt gaccttcagg ctgatgccct gctctggaac gaaactaaaa
     2281 aggcattcag tgcagctcac ggccaggata ccaccagcaa aatcaccaac gttaaagatg
     2341 ccgacctgac ggctgacagc actgatgctg ttaacggctc tcagctgaaa accaccaacg
     2401 atgctgtggc gacgaatacc accaatatcg ccaataacac ttccaatatt gccactaaca
     2461 ccaccaacat ctctaacctg actgagacgg tgactaatct tggtgaggat gcgctgaaat
     2521 gggataagga caatggtgta ttcacggcag ctcatggcac cgagaccacc agcaaaataa
     2581 ccaacgttaa agatggcgac ctgacgactg gcagcaccga tgccgttaac ggctctcagc
     2641 tgaaaaccac caacgatgcc gtggcgacga ataccaccaa tatcgccact aacaccacca
     2701 acatctctaa tctgactgag acggtgacta atcttggtga ggatgcgctg aaatgggata
     2761 aggacaatgg tgtcttcact gcagctcatg gcaacaatac cgccagcaaa atcaccaata
     2821 tcctggacgg cacagtcact gcaaccagtt ccgatgccat taacggtagc cagctttatg
     2881 acttaagcag caatatcgcc acctacttcg gcggcaatgc ttctgtgaat actgacggtg
     2941 tgtttaccgg tccaacctac aaaatcggtg aaacaaatta ttataacgtc ggcgatgcac
     3001 tggctgcgat taactcctca tttagcacgt ctctcggcga tgctctgctt tgggatgcca
     3061 ccgcaggtaa attcagtgcc aaacacggta ctaatggtga cgcaagcgtg atcactgatg
     3121 tcgcagatgg tgaaatttca gactccagtt ctgacgcagt aaacggctca caactccacg
     3181 gcgtgagcag ttatgttgtt gatgcgctgg ggggtggtgc cgaagtcaat gcagacggca
     3241 ccatcactgc gccgacgtac accattgcta atgctgatta cgataatgtc ggtgatgccc
     3301 tgaatgctat cgataccact cctgacgacg ctctgctctg ggatgcggac gccggtgaaa
     3361 atggtgcatt tagcgccgct cacggaaaag ataaaactgc cagtgtaatc actaacgtcg
     3421 ctaacggtgc aatctctgct gccagcagcg acgcgattaa cggctcacaa ctctatacca
     3481 ccaataagta catcgctgat gcgctgggtg gtgacgcaga agtcaacgct gacggcacca
     3541 tcaccgcacc gacttacacc attgcgaacg ccgagtacaa caacgtcggt gacgccctgg
     3601 atgcgcttga tgataacgcc ctgctgtggg ataagactgc caatggcggt gctggagcct
     3661 acaatgccag ccatgacggt aaagccagca tcatcactaa tgtcgctaat ggcagtatta
     3721 gtgaggacag taccgatgca gtgaacggtt ctcagttgaa tgcgacgaat atgatgattg
     3781 agcagaacac ccaaattatc aatcagctcg ctggtaacac cgacgcaacc tatatcgaag
     3841 aaaatggcgc tggtatcaac tacgttcgta ctaacgacaa cgatttagcc tttaacgatg
     3901 caagcgcctc tggtgtcggc gctacagctg taggttataa cgctgtcgcg tctggtgcca
     3961 gcagcgtagc cattggtcag aacagcagca gcaccgttga taccggtatt gcgctgggta
     4021 gcagctccgt ttccagccgt gtgattgcca aaggttctcg tgacactagc gtaacggaaa
     4081 atggcgtggt gattggttat gacaccactg acggcgaact gctaggtgca ttgtcaattg
     4141 gtgatgacgg taaataccgc caaatcatca acgtagctga tggttcagaa gcccatgacg
     4201 ccgttacggt tcgccagttg cagaacgcta ttggagcggt cgccactacg ccaaccaagt
     4261 actttcacgc caactcaacg gcagaagact cactggccgt tggtgaagac tcgctggcaa
     4321 tgggtgcgaa aactgtcgtt aatggtaatg caggtattgg tatcggtttg aacactctgg
     4381 ttctggctga tgcgatcaac ggcattgcta tcggcagtaa cgcacgcgca aaccatgcaa
     4441 acagtatcgc aatgggtaac ggttctcaga ccacccgtgg tgcacagact ggctacaccg
     4501 cctacaacat ggacgcaccg cagaactctg taggtgagtt ttctgtcggt agtgaagacg
     4561 gtcaacgtca gatcacaaac gtcgcagctg gttcagcgga taccgatgcg gttaacgtgg
     4621 gtcagttgaa agtcactgat gagcgcgtag cgcaaaatac ccagagcatt actaacctga
     4681 acaatcaggt cactaatctg gatactcgcg ttactaatat cgaaaacggt attggcgaca
     4741 ttgtcaccac cggtagcacc aagtacttca agaccaacac cgatggcgta gatgccaacg
     4801 cccagggtaa agatagcgtt gctattggtt ctggttccat tgctgccgct gacaacagcg
     4861 tcgcactggg taccggttcc gttgcagagg aagaaaatac aatctctgta ggttcttcta
     4921 ctaaccaacg ccgtatcacc aacgtagctg ccagtgttaa tgccaccgat gcggttaacg
     4981 tgtcgcagct gaaatcttct gaagcaggcg gagtgcgcta cgacaccaaa gctgatggtt
     5041 ctatcgacta tagcaatatc accctcggtg gtggcaacgg cagtacgact cgtatcagca
     5101 acgtctccgc tggcgtcaac aacaacgacg cggtgaacta cgcgcagttg aagcaaagcg
     5161 cgcaggaaac gaagcaatac accgatcagc ggatggttga gatggataac aaactgtcta
     5221 aaactgaaag caagttgagc ggtggtatcg cttctgcaat ggcaatgacc ggtctgccgc
     5281 aggcttatac accgggtgcc agcatggctt ctattggtgg cggtacttac aacggtgaat
     5341 cggcagttgc tttaggtgta tcgatggtga gcgccaatgg tcgttgggtc tacaaattac
     5401 aaggtagtac caatagccag ggtgaatact ccgccgcact cggtgccggt attcagtggt
     5461 aatgatccat taagttagtg tgactaaggg caaacgccaa attgcctgat gcgttacgcg
     5521 catcaggcaa gataaggcgc tccgggtcaa caatctaagg gcaattctct gatgaggatt
     5581 gcccttttct ttaccagaca tctcccccca caagaattgg ccctaccaat tcttcgctta
     5641 tctgacctct ggttcacaat tccccaatta aaactcacat caatattgcc aatacataac
     5701 atttagttaa ccattcattg tcattatccc tacacaacac aattggcagt gccactttta
     5761 cacaacgtgt gacaaggaga tgagcaacag actcattaca cgatgtgcgt ggactctagg
     5821 agacctgcaa tgaatctctg gcaacaaaac tacgatcccg ccgggaatat ctggctttcc
     5881 agtctgatag catcgcttcc catcctgttt ttcttctttg cgctgattaa gctcaaactg
     5941 aaaggatacg tcgccgcctc atggacggta gcaatcgccc ttgccgtggc tttgctgttc
     6001 tataaaatgc cggtcgctaa cgcgctggcc tcggtggttt atggcttctt ctatggtttg
     6061 tggcccatcg cgtggatcat tattgcagca gtgttcgtct ataagatctc ggtgaaaacc
     6121 gggcagtttg acatcattcg ctcgtctatt ctttcgataa cccctgacca gcgtctgcaa
     6181 atgctgatcg tcggtttctg tttcggcgct ttccttgaag gagccgcagg ctttggcgca
     6241 ccggtagcaa ttaccgccgc attgctggtc ggcctgggtt ttaaaccgct gtacgccgcc
     6301 ggactgtgcc tgattgttaa caccgcgcca gtggcatttg gcgcgatggg cattccaatc
     6361 ctggttgccg gacaagtaac aggcatcgac agctttgaga ttggccagat ggtggggcgt
     6421 cagctaccgt ttatgaccat tatcgtgctg ttctggatca tggcgattat ggacggctgg
     6481 cgcggtatca aagagacgtg gcctgcggtc gtggttgcag gcggctcgtt tgccatcgct
     6541 cagtacctta gctctaactt cattgggccg gagctgccgg acattatctc ttcgctggta
     6601 tcactgctct gcctgacgct gttcctcaaa cgctggcaac cagttcgcgt attccgcttc
     6661 ggtgacttgg gggcgtcaca ggttgatatg acgctggccc acaccggtta caccgcgggt
     6721 caggtgttac gtgcctggac accgttcctg ttcctgaccg ctaccgtaac actgtggagc
     6781 atcccgccgt ttaaagccct gttcgcatcg ggtggtgcgc tgtatgagtg ggtgatcaac
     6841 attccggtgc cgtacctcaa taaactggtt gcccgtatgc caccagtggt cagcgaggct
     6901 acagcctatg ccgccgtgtt taagtttgac tggttctctg ccaccggcac cgccattctg
     6961 tttgctgcac tgctctcgat tgtctggctg aagatgaaac cgtctgacgc tatcagcacc
     7021 ttcggcagca cgctgaaaga actggctcta cccatctact ccatcggcat ggtgctggcg
     7081 ttcgccttta tctcgaacta ttccggactg tcatcaacac tggcactggc actggcgcac
     7141 accggtcatg cattcacctt cttctcgccg ttcctcggct ggctgggggt attcctgacc
     7201 gggtcggata cctcatctaa cgccctgttc gccgcgctgc aagccaccgc agcacaacaa
     7261 attggcgtct ctgatctgtt gctggttgcc gccaatacca ccggtggcgt caccggtaag
     7321 atgatctccc cgcaatctat cgctatcgcc tgtgcggcgg taggcctggt gggcaaagag
     7381 tctgatttgt tccgctttac tgtcaaacac agcctgatct tcacctgtat ggtgggcgtg
     7441 atcaccacgc ttcaggctta tgtcttaacg tggatgattc cttaatgatt gttttaccca
     7501 gacgcctgtc agacgaggtt gccgatcgtg tgcgggcgct gattgatgaa aaaaacctgg
     7561 aagcgggcat gaagttgccc gctgagcgcc aactggcgat gcaactcggc gtatcacgta
     7621 attcactacg cgaggcgctg gcaaaactgg tgagcgaagg cgtgctgctc agtcgacgcg
     7681 gcggcgggac gtttattcgc tggcgtcatg acacatggtc ggagcaaaac atcgtccagc
     7741 cgctaaaaac gctgatggcc gatgatccgg attacagttt cgatattctg gaagcccgct
     7801 acgccattga agccagcacc gcatggcatg cggcaatgcg cgccacacct ggcgacaaag
     7861 aaaagattca gctttgcttt gaagcaacgc taagtgaaga cccggatatc gcctcacaag
     7921 cggacgttcg ttttcatctg gcgattgccg aagcctcaca taacatcgtg ctgctgcaaa
     7981 ccatgcgcgg tttcttcgat gtcctgcaat cctcagtgaa gcatagccgt cagcggatgt
     8041 atctggtgcc accggttttt tcacaactga ccgaacaaca tcaggctgtc attgacgcca
     8101 tttttgctgg tgatgccgac ggggcgcgta aagcaatgat ggcgcacctt agttttgttc
     8161 acaccaccat gaaacgattc gatgaagatc aggctcgcca cgcacggatt acccgcctgc
     8221 ccggtgagca taatgagcat tcgagggaga aaaacgcatg attatttccg cagccagcga
     8281 ttatcgcgcc gcagcgcaac gcattctgcc gccgttcctg ttccactata tggatggtgg
     8341 tgcatattct gaatacacgc tgcgccgcaa cgtggaagat ttgtcagaag tggcactgcg
     8401 ccagcgtatt ctgaaaaaca tgtccgactt aagcctggaa acgacgctgt ttaatgagaa
     8461 attgtcgatg ccggtagcac tggcaccggt gggtttgtgt ggcatgtatg cgcgtcgtgg
     8521 cgaagttcag gcagccaaag cggcggacgc gcatggcatt ccgtttactc tctcgacggt
     8581 ttccgtttgc ccgattgaag aagtcgcgcc agccatcaag cgcccaatgt ggttccagct
     8641 ttatgtactg cgcgatcgcg gctttatgcg taacgcgctg gagcgagcaa aagcagcggg
     8701 ttgttcgacg ctggttttca ccgtggatat gccgacaccg ggcgcacgct accgtgatgc
     8761 gcattcaggt atgagcggcc caaatgcggc aatgcgccgc tacttgcagg cggtgacgca
     8821 tccgcaatgg gcgtgggatg tgggcctgaa cggtcgtcca catgatttag gtaatatctc
     8881 agcttatctc ggcaaaccga ccggactgga agattacatc ggctggctgg ggaataactt
     8941 cgatccgtcc atctcatgga aagaccttga gtggatccgc gatttctggg atggcccgat
     9001 ggtgatcaaa gggatcctcg atccggaaga tgcgcgcgat gcagtacgtt ttggtgctga
     9061 tggaattgtg gtttctaacc acggtggccg ccagctggac ggtgtactct cttccgctcg
     9121 tgcgttgccc gctattgcag atgcggtgaa aggtgatatc gccattctgg cggatagcgg
     9181 aattcgtaac gggcttgatg tcgtgcgtat gattgcgctc ggtgccgaca ccgtactgct
     9241 gggtcgtgct ttcctgtatg cgctggcaac agcgggccag gcgggtgtag ctaacctgct
     9301 aaatctgatc gaaaaagaga tgaaagtggc gatgacgctg actggcgcga aatcgatcag
     9361 tgaaattacg caagattcgc tggtgcaggg gctgggtaaa gagttgccta cggcactggc
     9421 tccgatggcg aaagggaatg cggcatagtc gtttgcgccc cctcacccta accctctccc
     9481 tcagggagag gggaccgttc ggcgctgtat gtactccctc actctgaaac gacaccgcac
     9541 tctttttttc tccctcgccc ctccggggag ggccggggtg aggggaaaag gccgcacggt
     9601 tcaccaattt tctgctatcc tgcccccgca ctaagggggc agtatgctaa acatcgtact
     9661 ttacgaacca gaaattccgc caaatactgg caacatcatc cgtctttgcg ctaataccgg
     9721 ctttcgtcta catatcatcg aaccgatggg atttgcctgg gacgacaagc gcctgcgccg
     9781 cgcggggctg gactatcacg agtttaccgc cgttacgcgt catcatgact atcgcgcgtt
     9841 cctcgaagca gaaaatcccc agcgcctgtt tgccctcacc acgaaaggta cgcctgctca
     9901 cagcgccgta agctatcagg atggcgatta tctgatgttc ggcccggaaa cacgcggcct
     9961 gccagcgagc attcttgatg ccctgcccgc tgaacaaaaa attcgcattc cgatggtgcc
    10021 ggacagccgc agcatgaatc tgtccaatgc ggtgtcggta gtggtgtatg aagcctggcg
    10081 gcagctggga tatccgggag cgttgttgag agattagtta ctgtatgccg gatagatgat
    10141 tacatcgcat ccggcacgat cacaggacat tagatcccat ccccatactc aaatgtatgg
    10201 ttaataccgt tgaaatgctg gtccatatcc attgatggct tatcgctgtc tggtttaccg
    10261 acaatacgag ccggaacgcc agcggcggtt gtatgcggcg gcaccggttg cagcaccacg
    10321 gaacctgcgc caatcttcgc gccgcaacca acttcaatat tgccgaggat tttcgcgccc
    10381 gcgccaatca tcacaccttc acgaattttc gggtgacggt caccaccaga tttacccgta
    10441 ccgccaagcg tcacagattg cagaatcgat acgtcgtttt caattaccgc cgtttcaccc
    10501 acaacgatgc ccgtggcgtg gtcgagcata atgccacggc caatttttgc tgccgggtga
    10561 atatcgacct ggaacgtcac agaaacctgg ttttgcagaa agattgccaa tgcgcgacgc
    10621 ccctgattcc acaaccagtg accgatgcga taggcctgca aggcatgaaa acccttcagg
    10681 tataacaacg gggttgagta tttatcgact gccgggtcgc gggtacgcac cgcctgaata
    10741 tcacaggccg cagaggcgat catttccggg tcagcggcgt aggcttcttc aaccacttca
    10801 cggatagcaa tagcaggcat aattggcgat gacagcttgt tcgccagcat gtagctcagt
    10861 gcactgccaa ggttttcgtg cttgagtagc gtcgcgtggt aaaaactggc cagcattggc
    10921 tcacagtccg ccagcgttct ggcttcggct ttaatattgt tccagacaat ttccagttct
    10981 tcacacgaca ttgcttactc cacacgatga gataatgacc ggcccgttct gcgcgggccg
    11041 ggtcatagcg gtaacaaagg ttccctgggg ttagtgactg ctgcgctcgt ccttgcgtgc
    11101 acgacctaat aatgtcaatg ctgcctcgcg cgcgtttttt ccgcaatata atacttgata
    11161 aatttcctcg gttattggca tttcaacgcc gaagcgatgc gccagttcgc ggacttcttt
    11221 cgtattgcgg tagccttcca ccacctgacc aatcttctcc tgcgcgcttt gtacatccat
    11281 gccctgaccg agcatcatac caaaacggcg gttacgcgac tggttgtcgg tacaggtaag
    11341 caccagatcg cccagccccg ccatgcccat aaaggtggca ggatcggcac ccagcgccgc
    11401 accaagacgc gacatttcag ccagcccacg ggtgatcagc gccgtacgcg cattcgcacc
    11461 aaaaccgata ccgtcagaca tccccgcgcc aatggcaatg acgtttttca ccgcgccgcc
    11521 aagctgcacg ccaatgaaat ccgggttgct gtaaacgcgg aaacttttgc cgcagtgcag
    11581 cagctgctgg agatcatcgg caaaggtctg atcggtcgag gccagcgaaa tagctgtcgg
    11641 taaacctgcc gccagttctt tcgcaaacgt tgggccagag ataaccgcca gcggaatttg
    11701 atcgcctaag gcctcacgcg ccacgtcctg taacagacgt ccggtttccg cttccagccc
    11761 tttggtcgcc cacaccagac gcgcatcagg acgcatcagc ggtttaatct ggcgcagcac
    11821 ttcaccaaag acatggctgg gtacgacgac gagaatatta cgactggctg ccagcgcagt
    11881 ggcgagatcg ctttcaagat ggagcgtatc gggaaaaggc acatcgggga gaaacgcggc
    11941 gttacagcgg tcgcgttcaa gcgttgcgat atgttcaggg tcatggcccc agaggacaac
    12001 ctcgtggcca tttcttgcca gggtaatggc aagagcggtg ccgtacgagc cggcaccgat
    12061 cacagtcatt gaagcattac gttggttcat caggcatcct gatgttcttc agtaccttcg
    12121 ccagcctgct gctgcaaata gttcatgaac agcgcatcga agttaaccgg cgcaaggttc
    12181 agttgcggga atgtaccgcg ggataccatg ctggtgatgc actcacgagc atacgggaac
    12241 agaatgttcg ggcagtatgc gcccaggcaa tgtgccatct gggtgccttc gatacccgcg
    12301 atggagaaaa taccgccctg ctgaacttca cacaggaacg cggtttcttc gcccaaggag
    12361 gccgttacgg taacacgcag taccacttcg tatacgtcat ctgccagttg ggaagatgcc
    12421 gtatccagat caagtttaac ttctggttgc caatctttct ggaaaacgtg cggcgcgttc
    12481 ggcgcttcga aagagatatc cttggtataa atacgttgga tctggaaagt catttcagtg
    12541 ttgttttgtt ctgacatgtg tagaaaaccc ttaagtgttg tccttaaata ctgcgcaatg
    12601 taataccaac gttcacacgt tatttcagca ggggatccag tccaccacgt gcatccaatg
    12661 catacaagtc atcacagccg ccaatgtgct gtgcgtcaat aaaaatctgg ggaaccgtgg
    12721 tgcaaccgct gcgtttgatc atctcttcac gcttggcggc attgccatcg atcggcaact
    12781 cctggaaact cacgcccttg ctgctcagca gtgcttttgc acgatggcaa tacgggcagg
    12841 tttctttggt atagatttca acattggcca taatttagct cctgtttttt taccctgaaa
    12901 tttcatgttg cagggcggct gcaaaatgga gtgtccccag aagcttataa caataagtcg
    12961 ctgggggcct aattgcagct aacggcctgc atcatgaaag acgacaggta aattatttgc
    13021 cgcgcaccag aggcaagttt tcgccagccc agccagcaac gccctctttc agcacgaata
    13081 cttgcgcaaa accagctttc gtcagcgcgt ttgcaggctc ctggcactgc atgccagaac
    13141 cgtctaccac gataaccgat ttgtctttgt gcttctcaag ctcaccaaca ttgttggctt
    13201 tgatttcgct cggcaacaga ttgatagaac ctgcgatatg gcctttacgg aagtcatcac
    13261 gctgacgtaa atccacaacc acagcgtctt ctttgttgat cagacgcgta gcttcaccac
    13321 gagtaatcac cttcactttc gaggtcaggc tcttaaacgt agtcacaaga accgccacca
    13381 gtaacgcgat ccaggcgata ctcagtatgg gatgacggcc aacaaattgc ataatttctt
    13441 gcatgggggg taacaactcc cgacgtagtg attaaaaaaa ccaggaaagg agtatacctg
    13501 cgcggtgcgg caaatacagc cagcgcgtta actggaatgc aattttgcgg ggcacgacga
    13561 aaaaaaagcg caaatctggc atcggcttgc cgcgagcggt cgtatatttt gatcttcaga
    13621 ggctatttta tcgattcagc tgtagtaaaa ttacgcaaat tttgactctt gagtatgagg
    13681 ttgtcgcaat gtcggtttct aaaaaaccta tggtactggt gattctggat ggctatggct
    13741 atcgtgaaga acagcaggat aacgccattt ttagtgctaa aaccccggta atggatgcac
    13801 tgtgggccaa tcgtccgcat accctgatcg acgcttccgg tctggaagtc ggtctgcctg
    13861 accgtcagat gggtaactcc gaagtaggcc acgttaacct gggtgccggc cgcatcgtgt
    13921 atcaggacct gactcgtctg gacgttgaaa tcaaagatcg cgctttcttt gctaatccag
    13981 tgctggctgg cgcggtacat aaagcgaaaa acgcaggtaa agcagtacac attatgggtc
    14041 tgctctccgc aggcggcgta cacagccacg aagatcacat catggcgatg gtagaactgg
    14101 cagctgaacg cggcgcagaa aaaatctacc tgcacgcatt ccttgacggt cgcgacaccc
    14161 cgccgcgcag tgctgaatcc tcgctgaaaa aatttgaaga gaaatttgct gcgctgggta
    14221 aaggccgcgt agcgtccatc attggtcgtt actacgcgat ggaccgcgat aaccgttggg
    14281 atcgcgttga aaaagcctat gacctgctga ctctggcgca gggtgagttc caggccgata
    14341 ctgccgttgc tggtttgcag gcagcttatg ctcgcgacga aaacgacgag ttcgtgaaag
    14401 cgaccgttat tcgtgcagaa ggtcagccag atgcggcaat ggaagacggc gatgcgctga
    14461 ttttcatgaa cttccgtgct gaccgcgcgc gtgaaatcac tcgtgcattc gtgaacgctg
    14521 atttcgatgg cttcgcgcgt aagaaagtgg ttaacgtcga tttcgtgatg ctgaccgaat
    14581 acgccgctga catcaaaact gcggttgctt acccacccgc ttccctggtt aacaccttcg
    14641 gcgagtggat ggcgaaaaac gacaaaactc agttgcgtat ttccgaaacc gaaaaatatg
    14701 cccacgttac tttcttcttc aacggtggcg tagaagagtc gttcaaaggc gaagatcgca
    14761 ttctgatcaa ctcaccgaaa gtggctacct acgatctgca accggaaatg agctccgcag
    14821 agctgaccga aaaactggtt gcggccatca agagcggcaa atacgacacc attatctgta
    14881 actatccgaa cggcgacatg gtaggtcaca ccggggtgat ggaagcggcg gttaaagcgg
    14941 ttgaagcact ggatcactgc gtggaagaag tcgcgaaagc ggttgagtcc gtgggtggac
    15001 aactgctgat caccgctgac cacggtaacg ctgaacagat gcgcgatccg gcaacgggtc
    15061 aggcacacac ggcacacacc aacctgccag ttccgctgat ttacgttggt gataagaacg
    15121 tgaaagcggt tgcaggcggc aaactttctg acatcgcgcc gaccatgttg tcgctgatgg
    15181 gtatggaaat cccgcaagag atgactggta agccgctgtt catcgtggaa taatccctcc
    15241 ccatgagggg aaaggcgatt aataccatga cacgggccgt gaaaccgcgc aggtttgcaa
    15301 tcaggcccat catctacgcc agcgttctta gcgctggcgt attgttgtgc gccttttccg
    15361 cccacgcgga tgagcgtgac caactcaaat ctattcaggc cgatatcgcc gcaaaagagc
    15421 gcgcggtacg ccaaaagcaa caacaacgcg caagcctgct cgcacaattg aaaaagcagg
    15481 aagaagcgat ctctgaagcc acccgtaagc tgcgcgaaac gcaaaacacg ctcaatcaac
    15541 tcaataaaca gattgatgag atgaacgcgt cgattgccaa actggagcag caaaaagccg
    15601 cccaggagcg cagcctcgcc gcacaactgg atgccgcatt ccgtcagggc gagcataccg
    15661 gtattcagct aattctcagc ggtgaagaaa gccagcgtgg acagcgttta caggcttatt
    15721 tcggctatct caaccaggcg cgacaagaaa ccattgccca gttgaagcaa acgcgtgaag
    15781 aagtcaccat gcagcatgct gaactggaag agaaacaaag cgagcaacaa acgctgttat
    15841 atgaacagcg cgcccaacag gcgaaactga ctcaggctct gaacgagcgt aaaaagacgc
    15901 tggcggggct ggaatcttcc atccagcaag gtcagcagca gctaagtgaa ctgcgtgcca
    15961 acgaatcccg tctgcgtaac agcattgccc gtgctgaagc cgcggcgaaa gcgcgtgctg
    16021 aacgagaagc gcgtgaggcc caggcggttc gcgagcgcca gaaagaagcg acgcgcaaag
    16081 gcaccaccta caaaccgacc gaaagcgaaa aatcgctgat gtcccgaact ggtggcctgg
    16141 gtgcgccgcg tggtcaggca ttctggccgg ttcgcgggcc gacgctgcat cgctatggcg
    16201 agcagctaca gggtgaatta cgctggaaag ggatggttat cggtgcctct gaaggtactg
    16261 aagttaaagc aattgccgac ggtcgagtga ttctggctga ctggctgcaa ggttacggtc
    16321 tggtggtggt ggttgagcac ggtaaaggcg acatgagtct ttacggctat aatcagagcg
    16381 cactggtgag cgttggttcg caggttcgcg cgggccagcc aattgcactg gtgggcagca
    16441 gtggcggtca gggtcggcct tcactctatt tcgaaattcg ccgccagggt caggcggtca
    16501 atccacagcc gtggttggga agataagttt tgtttccatt tcgtcgtaac gttcttgcat
    16561 ttgccgctct gttggcgctc tcctcccccg tacttgctgg caaacttgcc atcgtcattg
    16621 atgattttgg gtatcgcccg cacaacgaaa accaggtgct ggcgatgcct tccgctatct
    16681 ccgtcgctgt attacccgat tcaccgcacg ccagagagat ggcgaccaaa gcgcataaca
    16741 gcggtcacga agtgttgatt catctcccga tggcaccgtt gagtaaacag ccgctggaga
    16801 aaaatacgct acgcccggag atgagcagcg acgaaattga gcgcattatt cgtagtgcgg
    16861 tcaataacgt gccctatgcc gtggggatca acaaccacat gggcagcaag atgacctcta
    16921 acctgtttgg tatgcagaaa gtgatgcagg cgctggagcg ttacaatctt tacttcctcg
    16981 acagcgtaac catcggtaat acccaggcga tgcgcgccgc gcaaggtact ggcgtgaagg
    17041 tgatcaaacg gaaggtgttc ctcgacgatt cgcagaacga agcggacatc cgtgtgcaat
    17101 ttaatcgcgc aattgatctg gcgcgtcgca acggttcaac cattgccatt gggcatcctc
    17161 acccttcaac ggtacgcgtg ctacaacaga tggtttataa cctgccgcca gacattacgc
    17221 tggtgaaagc cagcagcttg ctgaatgaac cgcaggttga tacttctaca ccgccgaaaa
    17281 acgctgtgcc tgatgcaccg cgtaatccgt tccgtggcgt gaagctgtgc aaaccgaaga
    17341 aaccgataga acctgtttat gctaatcgct tctttgaagt attaagcgaa agcatcagcc
    17401 agagcacgct gatcgtttac ttccagcatc agtggcaagg ctggggcaaa cagcccgaag
    17461 cggcgaagtt taacgctagc gcaaattaaa ggcgctacgc gtaatgcgat ggtgcgaccg
    17521 cgttttgtcg cgccattgcc ataagcggaa agaccacagt aacgcctggt aaccgacctt
    17581 cacgctgcgc acattggtaa tcaggcgctt atacataccc ggaagtaaat atctcggcaa
    17641 tcatccgttg gcgggtaaga atatccggct ctttgcg
//
LOCUS       DAZPAB010000075        17385 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZPAB010000075 DAZPAB010000000
VERSION     DAZPAB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.075"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(41..772)
                     /gene="rlmB"
                     /locus_tag="ACHUPG_002187"
     CDS             complement(41..772)
                     /gene="rlmB"
                     /locus_tag="ACHUPG_002187"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR0861794.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(952..3393)
                     /gene="rnr"
                     /locus_tag="ACHUPG_002188"
     CDS             complement(952..3393)
                     /gene="rnr"
                     /locus_tag="ACHUPG_002188"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR0861795.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3432..3857)
                     /gene="nsrR"
                     /locus_tag="ACHUPG_002189"
     CDS             complement(3432..3857)
                     /gene="nsrR"
                     /locus_tag="ACHUPG_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR0861796.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4062..5360)
                     /gene="purA"
                     /locus_tag="ACHUPG_002190"
     CDS             complement(4062..5360)
                     /gene="purA"
                     /locus_tag="ACHUPG_002190"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR0861797.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5464..5661)
                     /locus_tag="ACHUPG_002191"
     CDS             complement(5464..5661)
                     /locus_tag="ACHUPG_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR0861798.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5743..6747)
                     /gene="hflC"
                     /locus_tag="ACHUPG_002192"
     CDS             complement(5743..6747)
                     /gene="hflC"
                     /locus_tag="ACHUPG_002192"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR0861799.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6750..8009)
                     /gene="hflK"
                     /locus_tag="ACHUPG_002193"
     CDS             complement(6750..8009)
                     /gene="hflK"
                     /locus_tag="ACHUPG_002193"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR0861800.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8095..9375)
                     /gene="hflX"
                     /locus_tag="ACHUPG_002194"
     CDS             complement(8095..9375)
                     /gene="hflX"
                     /locus_tag="ACHUPG_002194"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR0861801.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9451..9759)
                     /gene="hfq"
                     /locus_tag="ACHUPG_002195"
     CDS             complement(9451..9759)
                     /gene="hfq"
                     /locus_tag="ACHUPG_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR0861802.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9845..10795)
                     /gene="miaA"
                     /locus_tag="ACHUPG_002196"
     CDS             complement(9845..10795)
                     /gene="miaA"
                     /locus_tag="ACHUPG_002196"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR0861803.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10788..12635)
                     /gene="mutL"
                     /locus_tag="ACHUPG_002197"
     CDS             complement(10788..12635)
                     /gene="mutL"
                     /locus_tag="ACHUPG_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR0861804.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12645..13982)
                     /gene="amiB"
                     /locus_tag="ACHUPG_002198"
     CDS             complement(12645..13982)
                     /gene="amiB"
                     /locus_tag="ACHUPG_002198"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR0861805.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14001..14462)
                     /gene="tsaE"
                     /locus_tag="ACHUPG_002199"
     CDS             complement(14001..14462)
                     /gene="tsaE"
                     /locus_tag="ACHUPG_002199"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR0861806.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14434..15981)
                     /gene="nnr"
                     /locus_tag="ACHUPG_002200"
     CDS             complement(14434..15981)
                     /gene="nnr"
                     /locus_tag="ACHUPG_002200"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR0861807.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15980..17119
                     /gene="queG"
                     /locus_tag="ACHUPG_002201"
     CDS             15980..17119
                     /gene="queG"
                     /locus_tag="ACHUPG_002201"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR0861808.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17102..17155)
                     /locus_tag="ACHUPG_002202"
     CDS             complement(17102..17155)
                     /locus_tag="ACHUPG_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861809.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3942 a         4959 c         4367 g         4117 t
ORIGIN      
        1 aaccatccaa atctggatgg cttttcataa ttcagaggaa ctagctgcgc tgacgaaccg
       61 cttcaaataa gcaaattccg gttgcaaccg aaacgttcag ggaagaaaca cttcctgcca
      121 ttgggatgct gatcaactca tcgcaatgtt cacgggtcag gcgacgcata ccttcacctt
      181 ccgcgcccat caccagcgcc aggcgtccgg tcattttgct ctgatagagt gtatgatccg
      241 cctcgcctgc cgtaccgacg atccagatat tctcttcctg caacatacgc atggtgcgcg
      301 caaggttagt cacccgaatc agcggaacgc tttctgccgc gccgcaggct acttttttcg
      361 ccgtagcgtt gagctgtgcg gagcgatctt tcggcacaat caccgcatga acgcctgcgg
      421 cgtccgcact acgcaggcac gcgccgaggt tgtgcggatc ggttacgccg tcgaggatca
      481 gcaggaacgg ttgatcgagc gaagcgatca gatccggcag atcgttttcc tgatactgac
      541 gtcctggctt cacgcgggcg ataatgccct gatgcacggc accgtcgctt ttctcgtcga
      601 gatattggcg gtttgccaac tggataacca cgccctgaga ctcaagggcg tgaatcagcg
      661 gtaacagacg tttatcttca cggcctttta aaataaagac ttcctgaaaa cgttcagggg
      721 cgcgctccag cagggcctgc actgcgtgga tgccgtaaat catttcgctc attaatgtac
      781 tcgttgttta cgttttgcct gatgcactac gtttatccgg cctacatgat ctctgcaata
      841 tattgaattt gcgtgattat gtaggccgga taaggcgttc acgccgcatc cggcatgaac
      901 aaagcgcatt ttgtcagcaa tctaaccctc ttcttttaaa gagggtattg atcactctgc
      961 cacttttttc ttcgccgcac gcttcgcttt ggtcgctgcg gctattttct gcgttttcgc
     1021 cgatggcttt ttcgcttttc tcgcgtcttt cttcgccgct ttcggcttcg tttttttctc
     1081 accgcggaag gcgctgtctg gctcaaagtt taccttttta ccgacctgac aacgcttgcc
     1141 gccttttttg cctgcatcgc cttttttcgc tttctcgcgc gccgttttac cgacgttgcg
     1201 cggtgcgcgt tcactggaga tcagactaaa gtcgattttg cgctcgtcca tattaaccgc
     1261 ttcgacgcga acttccacgc gatcgcccag gcgataagtc tggccgctgg attcccccat
     1321 caggcgttgc cctacctggt caaagcgata gtagtcattg tccagcgaag agacatggac
     1381 cagaccatca atgaacaagt cgtccagacg gacgaagaag ccaaagccag tgacgctgga
     1441 aattacgcct ttaaagacgt tacctacctg gtcgagcatg aagtcacact tcagccagtc
     1501 agcgacatcg cgcgttgctt cgtcggcacg acgttccgcc atcgaacagt gctgacccag
     1561 ttgcaacatc tcttccatcg aataatggta gccgccggtt tcagtggtgt tgccctgatg
     1621 cccctgctct ttcgccagca gatatttaat ggcgcggtgc agcgtcaggt caggataacg
     1681 acgaatcggc gaagtaaagt gcgcatagga ctgcaatgcc agaccgaagt gaccacggtt
     1741 ttctggatcg taaatcgcct gtttcatcga gcgtagcagc atggtttgca gcatttctgc
     1801 atcaggacgg tcggcaaccg actccagcag ttccgcgtag tcacgcggtt ccggcttgtt
     1861 accacccggc agctccagcc ccagctccgc cagcactgaa cggaaagagg taatcgcttc
     1921 ggtgctcggc ttgtcgtgaa tacggaacag tgccggttct ttcgctttct caacgaaacg
     1981 cgccgccgag atattcgcca gaatcatgca ctcttcaatt aatttgtgcg cgtcgttacg
     2041 ctgggtctgt tcgatacgtt caatacggcg ttcagcgttg aaaatgaact tcgcttcttc
     2101 gctctcaaat gagatcccac cgcgttcttc acgggcttta tccagcactt tatagaggtt
     2161 atgcaactct tcgagatgct taaccagcgg ggcgtactgc tcgcgcagat cctgatcgcc
     2221 ctgcagaata tgccagactt tggtgtaggt cagacgcgcg tgagagctca tcaccgcttc
     2281 gtagaatttg tagcccgtca ggcggccttt cgacgaaacc gtcatctcgc acaccataca
     2341 caggcggtct acctgcgggt tgagcgaaca caggccgtta gagagcactt ccggcagcat
     2401 cgggataacc tgcgaaggga agtacaccga cgtgccacgg ttacgcgctt ctctgtccag
     2461 cggcgttggc ggacgcacat agtagctgac gtcggcaatc gcgacccata aacgccagcc
     2521 gccgccgcgt tttttctcgc agtaaactgc atcgtcaaag tcacgggcgt cttcgccatc
     2581 aatggtgacc agcggtaaat cgcgcaaatc gacacggccc gcttttgctt cttccggcac
     2641 ttcttctttc agccccgcaa cctgttgctc aacagcctgc ggccagatat acggaatttc
     2701 atgggtacgc agagcgatat caaccgccat gccggtgccc atattgtcgc ccagcacttc
     2761 gacgatttta cccaccgctt tggtgcggcg agtcggacgc tgagtcagtt cgactacgac
     2821 cacaaagccc atccgcgcgc ccatgatctg atcgggcggg attaagatat cgaagctcag
     2881 acggctatcg tcaggaacca caaagccgac gcccgcttcg gtaaagtagc gaccaacaat
     2941 ctggctggtt tttggcacca gtacgcggac aatacgcgct tcacgacgac ctttacggtc
     3001 agcacccagc ggctgcgcca gcacctgatc gccatgaatg caggttttca tctgctcgct
     3061 ggagagatac aaatcatctt tacgcccttc aacccgcaga aagccgtagc catcacggtg
     3121 accaataacg gtacctttca ccaggtcgag gcgttccggc agcgcatagc actgacgacg
     3181 agtgaagacc agttgaccat cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag
     3241 ctgctcttcg ccttcaatgt gcagttctac cgccagctca tcacggctgg ccggtttttc
     3301 acgtttggtt aaatgttcga ggataaattc ccgactaggg atgggattcg cgtatttttc
     3361 agcttcgcgt tcctggaaag gatcttgtga catctcggtt cctccgttgt catctctgat
     3421 gaagattttc gtcactccac cagcaataat ttataaagcg gttgattctc ttcaaccaaa
     3481 tcggcaagcg tgtagttatc cagttccgta agaaaacttt gcacggcctt agaaagcgcc
     3541 tgtttcaacc gacaggcagg tgtaatgtgg caaaactcac tgctgcaatt caccagcgat
     3601 aagggctcca gctcgcgcac cacatcacca atacgtatcg cactcgccgg tttacccagg
     3661 cgaatgccgc cattttttcc acgaacagca gtcacgtagc cggcacgact aagttgattg
     3721 attattttga ccatatgatt acgggagacg ccgtagacgt cagtcacttc agaaatactg
     3781 gtcatccgcc cttctggcaa tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg
     3841 aaactcgtta actgcacatc aacctcaaaa gggaaatcgg gaaaaaatac atttatattg
     3901 atgataaacc agccacaagc tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc
     3961 gaggatttaa cactgtgtcg gatagcgtaa tggcttaacc gacacagcaa tgtatcacac
     4021 tgttagcccg gcaggcaaaa tatctgccag gcgtaccaga attacgcgtc gaacgggtcg
     4081 cgcagaatca tggtttcagt acgatccgga ccggtagaga tgatatcgat cggcacaccg
     4141 gtcagctctt caatacgctt gatgtagttc agcgccgcct gcggcaggcc gctacgatct
     4201 ttcacgccga aagtggattc agaccagccc ggcatggttt cgtaaatcgg ctctacacct
     4261 ttccagtcgt cagctgccag cggagtggta gtcacttcgc gaccatccgg catacggtaa
     4321 gccacgcaga gtttaacctc tttcaggcca tccagaacgt ccagtttagt caggcagaag
     4381 ccagacaggg agttcagctg taccgcacga cgaacggcaa cggtgtccag ccagccggta
     4441 cgacgacgac gacccgtagt tgcaccgaat tcgttaccct gcttgcagag gaactcgcca
     4501 gtttcatcaa acagttcggc cgggaaagga cctgcaccta cacgagttga gtaagctttg
     4561 aggataccca gaacgtaatc aacataacgc gggcccaggc cggaaccggt cgccacgcca
     4621 ccagcagtgg tgttggaaga agttacgtac ggataagtac cgtggtcgat atccagcagc
     4681 gtaccctgcg caccttcgaa catgacgaaa tcgccacgct gacgcgcctg gtcgagcagg
     4741 tcagaaacgt caaccaccat agaagtcagg atgtcggcaa cagccatcgt atcatccaga
     4801 actttctggt aatcaaccgc ttcagctttg tagtagttaa ccaactggaa gttgtgatat
     4861 tccatcactt ctttcagttt ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc
     4921 agaccgcgac gtgccacttt atcttcataa gcaggcccga taccacgacc ggtggtgccg
     4981 atcgctttcg cgccacgcgc tttctcacgc gcgttatcca gagcaacgtg ataatcaagg
     5041 atcagcggac atgcttcaga cagcagcaga cgctcacgaa cggggatgcc acggtcttcc
     5101 agttctttca tctctttcat cagcgcggcc ggagacagca caacaccgtt accgatgatg
     5161 ctggttacat tctcgcggag aatacctgat ggaataagat ggagaacggt tttttcaccg
     5221 ttgattacga gagtatggcc tgcgttgtga ccgccctggt agcgtacaac atatttagcc
     5281 cgttcagtca gaagatcgac gatcttacct ttaccttcgt caccccattg ggtgcccagt
     5341 acgacgacgt tgttacccat ttttcaaaat caccgtttgc ttaaaaatgg attctaccat
     5401 cgctttttca gagttacagc actttttgca ctcaaaaatg acctcgatca gtctgttttt
     5461 tgttcagcca atcgttttcc tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc
     5521 accgccaaaa cgacgtaaaa tattatcggg caaattggtc atcgcagaga tcatcttctt
     5581 ccatgccttc gggtaaagca tcggccctaa accttccagt accaaaacca gggcaagcgc
     5641 cagccagatt gtcgaattca ttcattatcc ttatagaaaa agaaaaccac cgacatccct
     5701 gaggatgcgg tggctttatt gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt
     5761 cggcgtcttc atgtagcgga agaaatcgct atccgggctc atgaccatca cgtcctgatt
     5821 gccagagaag ctgttctcat aagcacgcag gctacggatg aatgcgtaga agtccggatc
     5881 tttactgaat gcatcagcaa acagtttggc tgcttcggca tcgccttcac cacgcataat
     5941 gcggccctga cgctcagctt ctgccagcgt tctggtcact tcatagtcgg cagtcgcgcg
     6001 cagtttttcc gcttcttcct gaccttgtga acggtgacga cgcgctaccg cttcacgctc
     6061 ggcgcgcata cggttgtaga tcgcttcaga cacttcggtc ggcaggttga tctgcttgat
     6121 acgcacatcg acaacttcaa tacccagcgc cgccatactg ttcgggttga tgaccggaac
     6181 tttgcccttc gtctcagccg ttacgcgctc tgccgcttcg gcaatggcgt tatctgctgc
     6241 cggggtagta acttcatctt ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc
     6301 gagggtcaga cgaccacggg aatcggtgac gatatctttc acgtccaggc gaccaatttc
     6361 agaacgcaga cggtcagaga acttacgttt caacagcact tccgcttgcg aaatgtcgcc
     6421 accacccgtt gccaggtagt aacggctgaa atcgctgatg cgccatttga tgtaagagtc
     6481 gacgatcagg tctttcttct ctttggtcac aaagcggtcg gcctggttgt ccatggtctg
     6541 aatacgtgcg tcgagcattt tcaccgtttc aatgaacggt atcttgaaat gcagaccagg
     6601 ctcataaacc aaaggtttgt tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat
     6661 accgcgctca ccttctttga cgacaaagac agacatgtaa agcactacca gcacgatgat
     6721 gataatcgcg ataactgact tacgcatcgt tattccccct gacgctggta gtcgttacgc
     6781 tgcgcgttgg cgcggcgttg gtccataata tcgccctgac tggtggacga cgtgttgctt
     6841 gctccactgg ttgtggagga agaggctggc ggcagacgca gcagattgct ggcaccgtta
     6901 tcgctcttcg ccgcaggggc gttaccacct ttcagcatct ggtctaacgg cagaaccatc
     6961 aggttgccac ctttatcgtt aaccagcact ttgcgggtgt tacccaacac tttttccatc
     7021 gtctcgatat acagacgctc gcgagtaatt tccggcgcgg ctttatattc cggcagaagt
     7081 ttagcaaagc gcgccacttc accctgagct tccaggatgg tctgggcctt gtacgcacgc
     7141 gcctcttcga ggatacgttg cgcctgaccg ttcgcacgcg gctgaacttc gttggaatac
     7201 gcttctgctt cacgaatgta ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac
     7261 gccgctttta cttcttccgg cggacgagca gcctggaagt tgacgtccag cagcgtgata
     7321 cccatgtcat acggacgaat cgtctcttcc agttcgcgct gagtatcgct acgaatcacg
     7381 gtacgacctt ccgtcagaat gcggtccatg gtgtatttac cgataactcc acgcagggcg
     7441 ctgtcggtag cctgacgcag gctgtcatcc gggctggtca cgctatacag atatttttcc
     7501 ggattggtga cgcggtactg cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc
     7561 agcatcacac cagaagcggc cagttcacgc acggcttcca cgttcaccgg tttgacttcg
     7621 tcgataaacg tcggtttcca gttcagaccc ggctcaacca gatggctgaa tttaccaaag
     7681 cgtgttacca cgccgcgttc ggcttcttta atggtataga aaccactggc cgcccagata
     7741 atgacaatcg ctgccgctgc gatggtaacg acacgaccgc caagctgcgg gcgcgggcct
     7801 tgcgatgaac tgccaccgcc agatccggtg cctttaccgc cgcccagacc accgagcttt
     7861 ttgctcagtt tgcggaagat atcatctaaa tcaggtggcc cttgatcgcg accgcctttg
     7921 tttccatttc cctcagagtt gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt
     7981 ccgttattac cgggctgatt ccacgccatg tttgtgctcc atatttgtta tgcggtgatc
     8041 cctgttgtct ttcgacgcca gaggatatga ctccacgctt cagacgctac gccgttagat
     8101 caggtaatcg atcaacgccg gttcttgttt acagaggcga cgccagtcaa cgatcggcat
     8161 acgaacttgc agacttacgc tgccgtcctc ctccatccac tctttttcta ttgcctgaag
     8221 ctgataaaaa cgactcctca gacgcccttc ctgcggtggc agacgcaatg tatgctgcgc
     8281 cacctcgccg gaaagccgct ccgtcaaagc ctgaaaaagc tgtggtatcc ccgctccggt
     8341 ctgtgcggaa agccagacac ggatcggttt gttctcttcg tcccgatcaa tacgcggttc
     8401 gaaatcttcc agcatatcga tcttgttcat caccagcagg gttgggatct cgtgagcgtc
     8461 gatctcttca agaaccgtat tcaccgcttc gatgttttct tgtacacgca catccgccgc
     8521 atcaatgacg tgcagcagta atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc
     8581 cgccaccaga tcgtgcggca ggtggcgaat aaaccctacg gtatctgcaa gtacggtttc
     8641 accgacatct gcaacgtcaa tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc
     8701 tgccgcgtag acccgcgctt cggtgatgcg attgaaaagg gtagatttac cggcgttggt
     8761 atatcccacc agcgaaacag taggaacgtc ggctttgata cgcgattgcc gcccctgctc
     8821 acgctgcttt tcaactcttt ccaggcgcga ctgtatctgc acgatgcgat tacgcaacaa
     8881 acgacggtcg gtttcgagct gggtttcacc cggaccacgc aaacctatcc cgcctttctg
     8941 tctttcaagg tgggtccagc cacgcaccag gcgcgtagcc agatggcgca gctgcgccag
     9001 ctcaacctgc aacttaccct catgggtacg cgcacgttgg gcgaaaatat ctaaaataag
     9061 gccggtgcgg tcgataacac gacactcgca caaacgctcc aggttacgct cttgcgccgg
     9121 gctcagggca tggtcaaaaa gaacgaccga agcacccatc gctttgacag cttccgcaat
     9181 ttcaactgct ttaccttcac ctacaaaata ctttgggtgc ggcgctttac ggctaccggt
     9241 aatcacctgc aatgcttcga caccggcgga agagaccaga gattcaaact cctggaggtc
     9301 ttccatatct ttgtcttgcg taaaatagat gtgtaccagt accgcctgct caccagcatc
     9361 ataacggtca aacaagcgta taaccctcta aatagatcag cggggaacgc aggatcgctg
     9421 gctccccgtg taaaaaaaca gcccgaaacc ttattcggtt tcttcgctgt cctgttgcgc
     9481 ggaagtattc tgcgcgctgc taccatgatg gtagttactg ctggtaccgc caccggcgtt
     9541 gttactgtga tgagaaaccg ggcgagatgg gacaacagta gaaatcgcgt gcttgtaaac
     9601 catctggctg accgtgtttt tcaacaggat cacgaactga tcaaaagact cgatttgccc
     9661 ttgcagctta ataccattca ccaaataaat agaaactgga acacgttccc gacgcagtgc
     9721 gttcaggaac ggatcttgta aagattgccc cttagccatt ctctcttttc cttatatgct
     9781 tatttgtact ttgaaccttt cgattctgaa aaaattgcgc acgatacgtc tcaattgtac
     9841 acattcagcc tgcgatagca ccaacaacct gtaatacttc gtcacgcgcc tgttctggtt
     9901 tttcactgtc aagccagtga accccttccc aaccacgcag ccaggttatc tgccgcttcg
     9961 ccaactgtct cgtggcgcaa acacctcgat aaaccatttc atcgtatgag atttcgcctt
    10021 caaggtaaga ccacatctgg cgataaccca cgcaacgaat ggaaggcaag tccgtatgca
    10081 aatctcctcg ggcaaaaagc gcccggactt ctgcttcaaa acctgaagcc aacatctgat
    10141 gaaaacgctg ctcaatgcgt tgatggagca gttcacggct cgccggggcg atggcgaact
    10201 gatgcacctg atacggtaga gcgtctcctg acgtttgcgt cagttccgtt aaagttttac
    10261 ccgaaatgaa aaaaacttcc agtgcccggg aaagcctttg tggatcattt ggatgaatcc
    10321 ttgctgccgc aaccggatct acctcctgaa gttgacgatg caatgactcc caaccttgct
    10381 ctgccgcctg ttgctcaatt ctggcccgca cttccgggtc tgccgacggt agcggcgaca
    10441 acccttccag caatgccttg aaatacaaca tcgtaccgcc cactaacagt gggatccgcc
    10501 ccgccgcggt gatatcggcc atttccgcca gcgcatcgcg gcgaaaatca gcagccgagt
    10561 aagcctgcga cggatcgcga atatccagca atcggtgcgg cgcggcgagt aactcttcag
    10621 cgttcggctt cgccgtcccg atatccatcc ctttgtaaat aaggacagaa tcaacgctta
    10681 tcaactctac tggtaaaatt ttacgcagct caatggctaa cgccgtttta ccggaggccg
    10741 tcggccccat caaaaaaatc gccttaggca ggctcgcctt actgatatca ctcatctttc
    10801 agggctttta tcgccggatg taaatcaaca gattgtaaca gaccacccgg cggcgttttc
    10861 acaagttgcg gacataaccg ttccacgtcc gccagcaggg ttatggcctg tgccattgac
    10921 cactgcgcat gttcgctcat cagatttcgt gcaatccact gcgcaatatt gccaggttcg
    10981 aatacggact gcttcgccag gtagcctatc agttcaggaa tcaagatttg taaattttgt
    11041 tggcgtaagg gtaaaggcac ggccctgatg gtcacatgct gtgcatctga ctggaaatca
    11101 atacccaatt ccgctagggc agactgcgct ttttctaatg ccgatttttc ttcgccagaa
    11161 acttttagcc gcaacggaat cagcagcggc tgggcgcaaa cgggcgcttc acccggcgtc
    11221 aattgtgcct gacgcagcca acgttctgcc actggcaagg ataaaagtga aatgttgcca
    11281 tcgcgctcca gcaacgcaca gtcggaatgg acgatagtca gtacccgacc aaaactctga
    11341 ctgttcgccg caagtgcagg ttcctgcggt tccggcgctt ttggtttttg catcggcgcg
    11401 ggcgtttgca aaagctggcg atacacttca ccttgctgtt tctggtagcc tggctgcgca
    11461 ttcggccagg gggcagccgg acgactgcct gaggcgggcg caggagagta gcgcggagct
    11521 accggctcac gagctgccgg ttctgcaaag tgattgcgcc ccgccgccac gcggttttcc
    11581 gggatggcac gcggtgcggg ttgtggttca tcgtccagcg gtagcggcgt ttccagttgc
    11641 tgttgtagca cgctcagcac gccctgatag ataaaatcat gcaccagacg cgactgatgg
    11701 aaacgcactt cgtgtttggc ggggtgtacg ttgacgtcca cctgatgcgg gtcgatctcc
    11761 agatacaaca caaatgccgg ttgctgatcg gcccccagtt tgtcttcgca ggcctggcgg
    11821 atcgcatgat tgatcaggcg atcgcgcatc atgcgaccat tcacgtagca atactgaatt
    11881 tccgccagtg cgggcgtagt gtgatttgga tcggccaccc agccgcgcag ggtgagatcg
    11941 ccgtgttgcc attcaatcgc cagcgcttgt tcgagaaaag cggtgccgca aatcgcgcct
    12001 aagcgccgtt ctttttgccc gccttccggc actgcgcggt actgacgcac aattttaccg
    12061 ttatgcgaca ggttgatcgt gacgtcgaaa cgcgccagcg caatgcggcg gatgatttca
    12121 tcaatatggc taaattcggt tttctcggtg cgcaggaatt tgcgccgcgc cggggtgttg
    12181 tagaacagat ccagcacctc cagcgtcgtt cccaccggat gcgctgccgg tttaaccgtc
    12241 acgtccatgt cgcgtccttc ggcataggcc tgccaggctt cctgctgttc tgcggtgcgt
    12301 gaagtgagcg tcaggcggga aaccgaactg atactcgcca gcgcctcacc gcgaaagccc
    12361 aggctgataa tggcttcgag atcgtccaga gaggcgattt tactggtggc atgacgagcc
    12421 agcgccagcg ccagctcatc ttttttgata ccgcagccgt tatcacgaat gcggataagt
    12481 ttcgccccac cgcgttcgat atcaatatcg atacgcgtcg cacctgcatc gaggctgttt
    12541 tccactagtt ctttgactac cgacgcaggt cgctcgacca cctcacctgc ggcaatctgg
    12601 ttcgccagtt gtggcggtaa gacctgaatt ggcatcaatc gtccttagtt tggcagcgtg
    12661 cgatctggcg tcgtcaccgt actggcagtt tgtgccgttg caccctgcgg cgcagattgc
    12721 atcggatgcg caaggaagta attgcgcagg cctttgtaaa tggcttctgc cagctgttgt
    12781 tggtaatcgt cgctcgccag caaacgttct tcgctgttgt tgctgataaa accggtttcg
    12841 accagtactg atgggatatc cggcgagcgc agaacgccaa ggctggcgtg ttctggtcga
    12901 cgtttatgta tttcgccaat gcgttgcaac tgactgatca tactggtcgc tacatcatac
    12961 cctacccgct gggaatgacc gaactgtaaa tccagcaccg cctggcttaa ataggggtca
    13021 gactgactgt tcgccagcac atcacccgcc ccacccagca gctccgacag tttctcgtgc
    13081 tgctccagcc agctggccat ttcactgttg gcgcgacggt tagagagcac ccatacggaa
    13141 gcgccagtcg cactacggtt cggtgcggca tcagcgtgaa tcgacacgag gaaattggcg
    13201 ttttgcttat gtgccacatc actgcgcccc atcaccgaga taaagtaatc cccgtcacgg
    13261 gttaaaacgc ctttaaacat cggatcgtca ttgagcaaag tacgcaattt acgcgcgatg
    13321 gcgatggtga catttttctc ccgcgtacca ccggggccga tagcgccagg gtcctgaccg
    13381 ccgtgtccgg catcaatagc gatgataatt ttatcgccag tgttagccgt cgcgcgcgct
    13441 gccggacgcg ttaccgtatt actgctgata acacccgtag tgcggttact ttccgtttta
    13501 aacggattgc gcgccggttc gctgacgcgc ggtgcgacaa ccgcaggcgt ttcaacgcgt
    13561 ttcgcaacca cgggcggcgg aggaggcggt ggcggcacat cggcgttaat cgtaaagacg
    13621 acagtgtaat tgctgccatt ctgccgcttc accgcttcgg ttttaccgtt ttcggtaaga
    13681 tcgaccacca gccgtagcgt ttgtgcatct ttaggcgttc cagagcgaat cgccttcacc
    13741 agattattgc cgctgaacaa caacggcagt ccctgaatca tgcccgtttg tttgatatcg
    13801 agcgccacgg tgcgtttgct ttgatggcta aacgcataat caggatcgcc aataaaactc
    13861 aacgttatcc gcgcctgttg attaccgtta gaaacctgaa tatcagagag cgtcgcggca
    13921 cccaccggcg tgcacagcag cagcagcgtc gctaccaacc aatttctgat gcgatacatc
    13981 atcccgccac ctttcaaagg ttaaccggct aaacgcgcca gcaacaattc acccgcagag
    14041 gaaaccgcac tcacgcgcgc ctcacggcct tgtgcctgat aatcaatgtg tatttcgaca
    14101 tccgggtcag gaagaacacc tgtaccttgt tgtggccact ccaccaggca gatggcatcg
    14161 ttggcaaaat aatcgcggat ccccataaac tccagctcct cgggatcggc aaggcggtac
    14221 aaatcaaagt gatagaccat taagttgtcg agcgtatagg gttcgaccag cgtataagtg
    14281 gggcttttga cattaccctg atgacccaga gcctgtaaaa agccccggct aaaggtggtt
    14341 ttacctgcgc ctaaatcgcc atacagatag attacggttg cgccatcgca ggctttcgct
    14401 acccgctcgc ccaggtctaa tgttgcctgc tcatcaggga gcggaattac tcgattcatc
    14461 atggttttta tcagtcactt ccgggttaac aatacgctgt agcgtggaaa agagatcggt
    14521 tgccagcatc ccgcgcgttc caaaacgcgc cgccagtacg tcagctgccg caccgtgcgc
    14581 gacacagcct gcacaggctg catcatacgg cgacagtttt tgcccaagca atgcgccaat
    14641 aataccagag agcacatcgc ccatgccgcc gctcgccatg cctgcatttc cggcatcaat
    14701 aatgcctaaa gcgtcaggat gggcggcgac cacggttccg gcacctttca gcaccgctac
    14761 gccgccataa cgttgtacca gacgtttggc gcaatgtaag cggtcacttt caatttcagc
    14821 gacggaacag cctaacaacc gtgcggcctc gctaggatgc ggcgtgatca cgcgattgtg
    14881 acgcttatcg ggattgattg ccagcaggtt caatgcatcg gcatcccaca acatcggttt
    14941 gcgaaaattc tcaacttttt gcagggcttt tttcccccac tcttgctggc ccagaccggg
    15001 accaatcacc accacatcgg cccattccag gctttcggta agagagtcca tagtcagttc
    15061 atgcaccatc aattctggtc gtgcagtcag cagcggcgca atgttctcac tgcgggtcag
    15121 tactcggact aaaccagcac cagcacgtag cgccgcttcc cccgtcatac gaatagcccc
    15181 cgccgtgccg tgatcgccac cgataatcac cagccgcccg tgatcgcctt tatgcgaagt
    15241 cgggcgacgc ggtttcagcc agtgagaaag ttgttctgcc gaaaaccgct gaattttcgt
    15301 ctcctgacct gccagccagc tatccagccc cagtgagtca aaatgcagtt gtcctgtaac
    15361 atcccgcgct tttccagtga gcaagcctag tttcagcgca ataaaagtga tggtgtgatc
    15421 ggcgttgatc actgcgcctg gcgtagcgcc ggtttcagcc agcaggccgg aagggatatc
    15481 aaccgccaca atcggcgcag gatgggaatt agcgtggtcg attaactggc taatggattc
    15541 gcggggcgct tgctgcaagc cggtaccgag cagcgcatca acaatcagat ctaccgattc
    15601 gggccagaca atattcgaag catggatctc gccacccgcg tttaaccatg cttcgcgtgc
    15661 cagcgcggcc tcttccggca acggtttgtc gctctcctgg gccaacaacg tgacctcaat
    15721 gccgaccgct ttggccagtc gcgcgaccac gtagccatcg ccgccgttat taccatgacc
    15781 gcacagcacc agccagtggc gggcgtcagg atacgccgaa cgacacacct ggaatgcggc
    15841 ctcgccagcg cgaagcatca gctcatagag tgtgagcccc agcacatctg ccgcctcgcg
    15901 ttctccgcgg cggatatcgt cggcgtacca gacggtgtgt ggtatactta cggggttttt
    15961 cttcattgta tggtccgtca tgtcagagcc cctcgatctc aatcagttag cgcaaaaaat
    16021 taaacagtgg gggctggaac tgggctttca gcaggtaggt attaccgata ccgatctcag
    16081 cgagtccgag cccaaactgc aagcatggct ggacaaacaa taccacggcg aaatggactg
    16141 gatggcacgt cacggtatgc tgcgcgctcg cccccatgag ttattacccg gtacgctgcg
    16201 tgtaatcagc gtgcggatga attaccttcc tgctaacgcc gcatttgcca gcacgctgaa
    16261 aaaccccaaa ctcggctatg ttagccgtta tgcgctgggc cgtgactatc acaaacttct
    16321 gcgcaaccga ctcaaaaagc tgggcgagat gattcagcaa cattgtgttt cgctgaattt
    16381 tagaccgttt gtcgattctg cgcctattct cgagcgcccg ttagctgaaa aagctgggct
    16441 cggctggaca ggtaagcact cacttatcct caatcgcgag gccggttcgt tcttcttttt
    16501 aggcgaattg ctggtcgata ttccgctgcc cgtggatcaa ccagtcgagg aaggatgcgg
    16561 caaatgcgtg gcctgtatga cgatttgccc gaccggtgcc atcgtcgagc catataccgt
    16621 cgatgctcgc cgctgtatct cttatctcac catcgaactt gaaggggcgc tcccggaaga
    16681 gttgcgaccg ttaatgggaa accgtattta cggttgcgat gactgccagc ttatctgccc
    16741 gtggaatcgc tattcgcaac tcactacaga agacgatttc agcccgcgta agccgctaca
    16801 cgcaccggaa ctcattgagt tatttgcctg gagcgaagag aagtttttaa aagtcacgga
    16861 aggatcggcg attcgccgta taggtcacct gcgctggctg cgtaatatcg ccgtggcatt
    16921 aggcaatgcc ccttgggatg aaacgatttt gacagcgctg gaaagtcgta aaggtgagca
    16981 cccacttctc gatgagcaca tagcgtgggc gattgcgcag caaatagaga gacgaaatgc
    17041 gtgcatagtc gaagtgcaat tgccgaaaaa acagcgtctg gttcgggtga ttgaaaaagg
    17101 gttaccgcgt gacgcctgat tcatccacag cttgtgaata aaaataaaaa cgcattgcaa
    17161 ttcaagacga ataaaatcgt caagtgatcg actcgacaaa acggaatctt gatttttaac
    17221 ttatttatca ataggttaaa aaaataccat tcaccgtgcg aagtttcttt gaaattaaga
    17281 ttacagcctg aggctgtgga taactctgtt tacaagagtt tgtcagagac aacaaaaacc
    17341 agcaataacg catcgcgtcg ctgtagatat tttattgaga gaaga
//
LOCUS       DAZPAB010000076        17367 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZPAB010000076 DAZPAB010000000
VERSION     DAZPAB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.076"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..2095
                     /gene="pbpC"
                     /locus_tag="ACHUPG_002203"
     CDS             <1..2095
                     /gene="pbpC"
                     /locus_tag="ACHUPG_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFR0861810.1"
                     /translation="ARYLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGS
                     TLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGA
                     ASWAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWS
                     REQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNW
                     KGRLPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVY
                     GLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEA
                     YGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLR
                     LQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWA
                     IGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVT
                     RGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQ
                     RVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGV
                     RDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLV
                     MDDVGQIATVKFVMQ"
     gene            2245..2676
                     /gene="ndk"
                     /locus_tag="ACHUPG_002204"
     CDS             2245..2676
                     /gene="ndk"
                     /locus_tag="ACHUPG_002204"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR0861811.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2826..3980
                     /locus_tag="ACHUPG_002205"
     CDS             2826..3980
                     /locus_tag="ACHUPG_002205"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR0861812.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4265..5278
                     /gene="rodZ"
                     /locus_tag="ACHUPG_002206"
     CDS             4265..5278
                     /gene="rodZ"
                     /locus_tag="ACHUPG_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR0861813.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5305..6432
                     /gene="ispG"
                     /locus_tag="ACHUPG_002207"
                     /gene_synonym="gcpE"
     CDS             5305..6432
                     /gene="ispG"
                     /locus_tag="ACHUPG_002207"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR0861814.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6535..7809
                     /gene="hisS"
                     /locus_tag="ACHUPG_002208"
     CDS             6535..7809
                     /gene="hisS"
                     /locus_tag="ACHUPG_002208"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR0861815.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7827..8447
                     /gene="yfgM"
                     /locus_tag="ACHUPG_002209"
     CDS             7827..8447
                     /gene="yfgM"
                     /locus_tag="ACHUPG_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR0861816.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8458..9636
                     /gene="bamB"
                     /locus_tag="ACHUPG_002210"
     CDS             8458..9636
                     /gene="bamB"
                     /locus_tag="ACHUPG_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR0861817.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9754..11226
                     /gene="der"
                     /locus_tag="ACHUPG_002211"
     CDS             9754..11226
                     /gene="der"
                     /locus_tag="ACHUPG_002211"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR0861818.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11296..11511
                     /locus_tag="ACHUPG_002212"
     CDS             11296..11511
                     /locus_tag="ACHUPG_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR0861819.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="ACHUPG_002213"
     CDS             complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="ACHUPG_002213"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR0861820.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13040..14506
                     /gene="guaB"
                     /locus_tag="ACHUPG_002214"
     CDS             13040..14506
                     /gene="guaB"
                     /locus_tag="ACHUPG_002214"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR0861821.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14575..16152
                     /gene="guaA"
                     /locus_tag="ACHUPG_002215"
     CDS             14575..16152
                     /gene="guaA"
                     /locus_tag="ACHUPG_002215"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR0861822.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16247..16786)
                     /locus_tag="ACHUPG_002216"
     CDS             complement(16247..16786)
                     /locus_tag="ACHUPG_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR0861823.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16802..17320)
                     /locus_tag="ACHUPG_002217"
     CDS             complement(16802..17320)
                     /locus_tag="ACHUPG_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR0861824.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
BASE COUNT         4023 a         4320 c         4823 g         4201 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgta ataaagtgct cgaacggatg gctgcgcaag gtgtgtggtc
      481 ccgtgagcag gtaaaagagt caagggaaga acccatctgg ctggcacccc gacaaatgcc
      541 gcaactggca ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt
      601 tactacgctg gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg
      661 gcggttgcca ccgcgcagct cactggcgat gatcgtggtt aatcataccg atatgcgtgt
      721 tcgcggctgg gtgggatcgg ttgatctcaa cgatgattca cgctttggtc atgttgatat
      781 ggtcaatgcg atccgatcgc caggatcggt gctcaaaccg tttgtttatg gtctggcgct
      841 ggatgaaggc ttgatccacc cggcatcact gctgcaagac gtcccccggc gcaccggtga
      901 ttatcgacca ggtaactttg atagcggttt tcatggcccg atcaacatga gcgaggcgct
      961 ggtgcgctcg ctgaacttac ctgctgtgca ggtgctggaa gcctatggac cgaaacggtt
     1021 tgcggcaaag ttacgcaatg ttggattgcc gttatatttg cccaacggcg ctgcgccgaa
     1081 tctttcactc attctcggcg gcgctggtgc aaaactggaa gatatggcgg cagcgtatac
     1141 cgcgtttgct cgccacggca aggcaggcaa attgcgctta cagcctgacg atccgctgct
     1201 tgaacggcct ttaatgtcgt cgggggcggc gtggatcatt cgccggatta tggctgatga
     1261 agcgcaaccc ttgccggata gtgccttgcc gcgcgtcgcc ccactggcat ggaaaacggg
     1321 caccagctat ggctatcgtg acgcctgggc gattggggtt aacgctcgct atgtcattgg
     1381 gatctggact ggcagacctg acggcacgcc cgttgttggt cagtttggct ttgccagtgc
     1441 cgtaccattg ttgaatcagg tcaataatat cttactgtcg cgcagtgcga atctgccgga
     1501 agacccgcgc ccgaactcag tcactcgtgg cgttatctgc tggccgggcg gacagtcttt
     1561 gccggaaggt gacggtaact gtcgccgccg cctggcaacc tggctgctgg atggcagcca
     1621 gccgccaacg ctgttactgt cagagcagga agggattaat ggcattcgct tccccatctg
     1681 gctggatgac aaagggcagc gcgtcgccgc tgactgcccg caggcgaagc aagaaatggt
     1741 aaatgtctgg ccgctaccgc tggaaccctg gctgcccgca tcagaacgtc gcgctgtgcg
     1801 cttaccaccg gcctcgacaa tttgcccgcc atacggtcac gatgcccagc tccctctgca
     1861 gctgacaggc gttcgcgatg gcgcgattat taaacgttta ccgggcgcag cggaagcgac
     1921 tttgccgttg caatcaagtg gaggggcagg tgaacgctgg tggtttctga atggcgaacc
     1981 gttaactgaa cgcgggcgca acgtgaccct gcatttgacg gataaaggtg attatcaatt
     2041 gctggtgatg gatgatgtgg ggcaaatcgc gacagtgaaa tttgtcatgc aatagtctgt
     2101 tttgtccctg attgttgcta aaactcattt tattttaaaa aaaatgttac cttcatcaat
     2161 agtcaacggc cctgttgctc attataatcc gcgccatctc gtacgctggt acagacaaca
     2221 acagaacaat ttacagaggt aaaaatggct attgaacgta ctttttccat catcaaaccg
     2281 aacgcggtag caaaaaacgt cattggtaat atctttgcgc gctttgaagc tgcagggttc
     2341 aaaattgtcg gcaccaaaat gctgcatctg accgttgaac aggcacgtgg cttttatgct
     2401 gaacacgatg gaaaaccgtt ctttgatggt ctggttgaat tcatgacctc tggcccgatc
     2461 gtggtttccg tgctggaagg tgaaaacgcc gttcagcgtc accgcgatct gctgggcgcg
     2521 accaatccgg caaacgcact ggctggtact ctgcgcgctg attacgctga cagcctgacc
     2581 gaaaacggta cccacggttc tgattccgtc gaatctgccg ctcgcgaaat cgcttatttc
     2641 ttcggcgaag gcgaagtgtg cccgcgcacc cgttaataat ttcgtaaatg ccgcgtgcaa
     2701 acgtggcatc cgtgcgccag aatttgtaca atgcagcgcc cccggacgag cagccgctca
     2761 ccggggcgtt tcttttttca accctccagg ggccataacg tgtaataacg aggccggaat
     2821 agcatatgtc tgaacaatta gtcacacctg aaaacgtcac cacgaaagat ggaaaaatca
     2881 acctgctgga tctcaaccgt cagcagatgc gggagttttt taaagattta ggtgaaaaaa
     2941 ccttccgcgc cgatcaggtg atgaagtgga tgtatcacta ttgctgcgac aactttgatg
     3001 agatgaccga catcaacaaa gtgttgcgcg gcaaactgaa agaggtggcg gaaatccgcg
     3061 caccggaagt ggttgaagaa cagcgttcat ccgacggcac cattaaatgg gcgatcgccg
     3121 ttggcgatca gcgcgtcgaa acggtgtata tcccggaaga cgaccgtgcc acgctctgcg
     3181 tctcttcgca ggtggggtgt gcgctggagt gtaaattctg ttccaccgcc cagcagggct
     3241 ttaaccgcaa cctgcgggta tcggaaatta ttggccaggt gtggcgtgcg gcgaaaatcg
     3301 tcggcgcagc aaaagtcacc ggtcagcgcc caatcactaa cgtcgtgatg atgggcatgg
     3361 gcgagccgct gctcaacctg aacaacgtcg ttccggcgat ggaaatcatg cttgatgatt
     3421 tcggttttgg cctgtctaaa cgtcgcgtga cgctttccac ttccggtgta gttccggcgc
     3481 tggataaact gggcgatatg atcgacgttg cactggcaat ttccctgcac gcgccgaacg
     3541 acgaaattcg tgacgaaatt gtgccgatca acaaaaagta caatatcgaa acgttccttg
     3601 ctgcggtgcg ccgttatctg gagaaatcca acgccaatca gggccgagtc actattgaat
     3661 acgtgatgct tgaccacgtt aacgacggca ctgaacacgc gcaccaactg gcggaactgc
     3721 tgaaagatac gccgtgtaag atcaacctga tcccgtggaa cccgttcccg gacgcgccgt
     3781 atggacgcag ttcgaacagc cgtatcgacc gtttctcaaa agtgctgatg agctacggtt
     3841 tcaccactat tgtgcgtaaa acgcgtggtg atgatatcga tgctgcctgt ggtcagttgg
     3901 cgggcgatgt tatcgaccgt acgaaacgta ctctgcgtaa acggatgcag ggtgaagcca
     3961 tcgatattaa agcggtctga tagccattac gccacggtta ctttatgtac cgtggcgtaa
     4021 tcttttatga atgaagtatt aagttgcaat tgtctggtca atattaacgg tgcgtttttg
     4081 ctgactttaa ggcagtatgt aacgatgcaa cagtaagtta gcctcagtga atgtgggctt
     4141 tgtcacgagc acacagacgg tcttatactg tatgataacg gttaacttaa cggatgtttc
     4201 gcggtgtggg tgagcatgat ggttcaccgg catctcaatt ctcatttaaa cgtacctgca
     4261 gcgaatgaat actgaagcca cgcacgacca aaatgaagca cttactaccg gcgctcgcct
     4321 gcgtaatgct cgcgaacaac taggacttag tcagcaggcc gttgccgagc gactttgcct
     4381 gaaggtttcc acggtacgcg acattgaaga agataaggca cccgccgatc ttgcttcaac
     4441 attcctgcgc ggatatatcc gctcttatgc gcgtctggta catattccag aagaagaact
     4501 gctgccaggg ctggaaaagc aggctccact tcgggctgca aaagttgcgc caatgcagag
     4561 tttttccctc ggtaaacgcc gcaaaaaacg cgacggctgg ctaatgacct tcacctggct
     4621 ggtgttgttt gtggttatcg gcctgagcgg tgcctggtgg tggcaagacc acaaagctca
     4681 gcaggaagag atcaccacta tggccgatca atcttcggcg gaactgagca gtaatagcga
     4741 gcaggggcag agtgttccgt taaatacgtc gacaactaca gacccggcta caacgtccac
     4801 gccgccagcg tctgtggata ctaccgcaac caacacgcaa acacctgccg taactgcgcc
     4861 agcaccagct gttgatccgc aacagaatgc ggttgtttcg ccttcgcagg caaatgttga
     4921 taccgccgcg accccggtac caacggcagc aacaacgcca gatggtgctg cgccgttgcc
     4981 aaccgatcag gctggcgtga ccacgccggc ggctgatccg aatgcgctgg tgatgaactt
     5041 tactgccgat tgctggctgg aggtcactga tgctaccggt aaaaaattgt ttagcgggat
     5101 gcagcgtaaa gacggtaatt tgaatttaac cggccaggca ccgtataaac tgaaaattgg
     5161 tgcgccagcc gcagtacaga tccagtatca agggaaacct gtcgatctga gtcgttttat
     5221 cagaactaac caggttgcgc gtctgaccct caatgccgaa caatcaccgg cgcagtaaca
     5281 gacgggtaac gcgggagatt tttcatgcat aaccaggctc caattcaacg tagaaaatca
     5341 acacgtattt acgttgggaa tgtgccgatt ggcgatggtg ctcccatcgc cgtacagtcc
     5401 atgaccaata cgcgtacgac agatgtcgaa gcaacggtca atcaaatcaa ggcgctggaa
     5461 cgcgttggcg ctgatatcgt ccgtgtctcc gtaccgacga tggacgcggc agaagcgttc
     5521 aaactcatca aacagcgggt taacgtgccg ctggtggctg acatccactt cgactatcgc
     5581 attgcgctga aagtagcgga atacggcgtc gattgtctgc gtattaaccc tggtaatatc
     5641 ggtaatgaag agcgtattcg catggtggtt gactgtgcgc gcgataaaaa cattccgatc
     5701 cgtattggcg ttaacgccgg atcgctggaa aaagatctgc aagaaaagta tggcgaaccg
     5761 acgccgcagg cgttgctgga atccgccatg cgtcatgttg atcatctcga tcgcctgaac
     5821 ttcgatcagt tcaaagtcag cgtgaaagcg tctgacgtct tcctcgctgt tgagtcttat
     5881 cgtttgctgg caaaacagat cgatcagccg ctgcatctgg ggatcaccga agccggtggt
     5941 gcacgcagcg gggcagtaaa atccgccatt ggtttaggtc tgctgctgtc ggaaggcatc
     6001 ggcgacacgc tgcgtgtatc gctggcggcc gatccggtcg aagagatcaa agtcggtttc
     6061 gatattttga aatcgctgcg tatccgttcg cgagggatta acttcatcgc ctgcccgacc
     6121 tgttcgcgtc aggaatttga tgttatcggt acggttaacg cgctggagca acgcctggaa
     6181 gatatcatca ctccgatgga cgtttcgatt atcggctgcg tggtgaatgg cccaggtgag
     6241 gcgctggttt ctacactcgg cgtcaccggc ggcaacaaga aaagcggcct ctatgaagat
     6301 ggcgtgcgca aagaccgtct ggacaacaac gatatgatcg accagctgga agcacgcatt
     6361 cgtgcgaaag ccagtcagct ggacgaagcg cgtcgaattg acgttcagca ggttggaaaa
     6421 ataataacgt gatgggaagc gcctcgcttc ccgtgtatga ttgaacccgc atggctcccg
     6481 aaacattgag ggaagcgttg agggttcatt tttatattca gaaagagaat aaacgtggca
     6541 aaaaacattc aagccattcg cggcatgaac gattacctgc ctggcgaaac ggccatctgg
     6601 cagcgcattg aaggcacact gaaaaacgtg ctcggcagct acggttacag tgaaatccgc
     6661 ttgccgattg tagagcagac cccgctattc aaacgtgcga ttggtgaagt caccgacgtg
     6721 gttgaaaaag agatgtacac ctttgaggat cgcaatggcg acagcctgac tctgcgccct
     6781 gaagggacgg cgggctgtgt acgcgccggc atcgagcatg gtcttctgta caatcaggaa
     6841 cagcgtctgt ggtatatcgg gccgatgttc cgtcacgagc gtccgcagaa agggcgttat
     6901 cgtcagttcc atcagttggg ctgcgaagtt ttcggtctgc aaggtccgga tatcgacgct
     6961 gaactgatta tgctcactgc ccgctggtgg cgtgcgctgg gtatcttcga acacgtaact
     7021 cttgagctga attctatcgg ttcgctggaa gcacgcgcca attaccgcga tgcgctggtg
     7081 gcattccttg agcagcataa agaaaagctg gacgaagact gcaaacgccg catgtacact
     7141 aacccgctgc gcgtgctgga ttcaaaaaat ccggaagtgc aggcgcttct caacgacgct
     7201 ccggcattag gtgattatct ggacgaggaa tctcgtgagc attttgccgg tctgtgcaaa
     7261 ctgctggaga gcgcggggat cgcttacacc gtaaaccagc gtctggtgcg tggtctggat
     7321 tactataacc gtaccgtttt cgagtgggtg actaacagtc tcggctccca gggcaccgtg
     7381 tgtgcaggcg gtcgttatga cggtcttgtg gaacaactgg gcggtcgtgc aacaccggct
     7441 gtcggttttg cgatgggcct cgaacgtctt gtattgttag tacaggccgt taatccggaa
     7501 tttaaagccg atcctgttgt cgatatatac ctggtggctt caggtgctga tacacaatct
     7561 gcggctatgg cattagctga gcgtctgcgt gatgaattac tgggcgtgaa attgatgacc
     7621 aaccacggcg gcggcaactt taagaaacag tttgcccgtg ctgataaatg gggtgcccgc
     7681 gttgctgtgg tgctgggtga gtctgaagtg gctaacggca cagcagtagt gaaggatttg
     7741 cgctctggtg agcaaacggc agttgcgcag gatagcgtag ccgcgcattt gcgcacgtta
     7801 ctgggttaag gaaggagaag gacagcgtgg aaatttacga gaacgaaaac gaccaggtag
     7861 aagcggttaa acgctttttt gctgaaaatg gcaaagcact ggctgttggg gggattttgg
     7921 gcgttggcgc actgactggc tggcgctact ggaacagcca tcaggttgat tctgcacgct
     7981 ccgcttctct tgcctatcaa aatgcggtta ccgcagtgag cgaaggcaaa ccggatagca
     8041 tcccggcggc ggaaaaattt gctgctgaaa ataaaaatac ttatggtgcg ctggcttctt
     8101 tggaacttgc gcagcaattt gttgacaaaa atgaactaga gaaagctgcc gcccagttac
     8161 aacaggggct ggcagacacg agcgatgaaa atctcaaagc cgtgataaat ctgcgtcttg
     8221 ctcgcgttca ggtacagctc aagcaggctg atgccgcgct gaaaaccctt gataccatca
     8281 aaggtgaagg gtgggctgcc attgttgccg acttgcgtgg tgaagcattg ctgagcaaag
     8341 gtgataagca aggtgcgcgt agtgcatggg aagcaggcgt gaaaagcgat gttactccgg
     8401 cactgagcga aatgatgcag atgaaaatta ataatttgtc catctgagag ggacccgatg
     8461 caattgcgta aattactgct gccaggactg ctttccgtta cccttttaag cggctgttcg
     8521 ctgtttaaca gcgaagaaga tgtggtaaag atgtccccat tgccaaccgt tgaaaaccag
     8581 tttacgccga ccacggcgtg gagcacttcc gttggtagcg gcattggcaa cttctattcc
     8641 aatcttcatc cggcactggc ggacaacgtt gtctatgcag cggaccgcgc tggtttggta
     8701 aaagcgctga atgcggatga tggcaaagaa atctggtctg tcagcctggc cgagaaagat
     8761 ggctggttct ctaaagagcc tgcattactt tctggcggtg tgaccgtgtc tggtgggcat
     8821 gtctacattg gcagcgaaaa ggcgcaggtt tacgcgctga ataccagcga tggtactgtg
     8881 gcatggcaaa ctaaagtcgc gggtgaagca ctttcgcgcc cggtggtcag cgacggtctg
     8941 gtgttaatcc acaccagtaa cggtcagtta caagcgctga acgaagctga cggcgctgtc
     9001 aaatggacag ttaacctcga tatgccttcg ctctctttgc gtggcgagtc tgcgccggca
     9061 acggcttttg gtgcggccgt cgtgggtggc gataatggtc gcgtcagcgc agtgctgatg
     9121 gaacagggcc agatgatttg gcagcagcgt atttcccagg cgaccggttc taccgaaatt
     9181 gaccgtctga gcgatgttga cacgactccc gtcgttgtta acggcgttgt tttcgcgctg
     9241 gcctataatg gtaacctgac ggcgcttgat ctgcgcagtg gtcagattat gtggaaacgc
     9301 gaactgggtt cggtgaatga tttcatcgtc gatggcaatc gcatctatct ggtcgatcaa
     9361 aatgaccggg tgatggcgtt gaccattgat ggcggcgtta cgctgtgggc acaaagcgat
     9421 ctgctgcatc gcctgctgac ttctccggtg ctgtataatg gcaacctggt ggtcggtgac
     9481 agtgaaggtt atctgcactg gattaacgtc gaagatggtc gtttcgttgc ccagcaaaaa
     9541 gttgatagtt ccggtttcca gactgaaccg gttgccgctg acggcaaact gctgatccag
     9601 gcaaaagacg gaaccgtgta ctctattaca cgttaatcgt ctctgtcgtt cactttgaaa
     9661 acggctcctg gacaggggcc gttttcctgt ttttaacaac gacgcgaata tagcgtgcgt
     9721 tgtctgatga tttataaaaa tgaggcttta aacatggtac ctgtggtcgc gcttgtcggg
     9781 cgccctaacg taggaaaatc cacgttattt aaccgtctaa ctcgcacccg agatgcgctg
     9841 gttgcggatt tcccgggtct gactcgtgac cgtaagtacg gtcgtgcgga aattgaaggc
     9901 cgtgagttta tctgtattga taccggcggg attgatggca cagaagacgg tgtagaaacc
     9961 cgcatggcgg aacagtcgct gctggcgatt gaagaagcgg acgtcgtact gtttatggtg
    10021 gatgcgcgcg cgggcctgat gccggcagat gaagcgattg ccaaacatct gcgctcccgt
    10081 gaaaaaccga ccttcctggt ggtaaacaaa actgacggtc tggatcccga tcaggcagtg
    10141 gttgatttct actcgcttgg tttaggtgaa atctacccga tcgccgcgtc tcacggtcgt
    10201 ggcgtattaa gtctgctgga gcatgtgctg ctgccgtgga tggaagatct cgcaccgcaa
    10261 gaggaagtcg acgaagacgc tgaatactgg gcgcaatttg aagcggaaga gaacggcgaa
    10321 gaagaagagg aagacgactt cgacccgcaa agtctgccga tcaaactggc gattgtgggt
    10381 cgtccgaacg taggtaagtc tacactcact aaccgtattc ttggagaaga gcgcgttgtt
    10441 gtttacgaca tgcctggcac gacgcgtgac agcatctaca tcccaatgga acgcgatgga
    10501 cgtgagtatg tgctcattga caccgctggc gtacgtaaac gcggcaaaat caccgatgct
    10561 gtagagaaat tctccgtaat caaaacgttg caggccattg aagacgccaa cgtggtgatg
    10621 ttagtgattg atgcgcgcga aggtatttcc gatcaggatc tctcgctgct gggctttatt
    10681 ctcaatagtg ggcgctcact tgtcattgtg gtgaataagt gggatggcct gagccaggaa
    10741 gtgaaagagc aggtgaaaga aacgctggac ttccgtctgg gctttatcga ttttgctcgt
    10801 gtgcacttta tctctgcctt gcacggcagt ggtgttggta acttgtttga atcagtacgt
    10861 gaagcgtatg acagctccac ccgtcgtgtg gggacctcta tgctgacgcg catcatgacg
    10921 atggctgttg aagatcacca accgccgctg gtacgcggtc gtcgtgtgaa gctgaaatat
    10981 gcccacgctg gtggttataa cccgccgatt gtggtgattc acggtaatca ggtgaaagac
    11041 ctgcctgatt cctacaagcg ctacttgatg aactacttcc gcaaatcgct ggacgtaatg
    11101 ggatcgccga ttcgtattca gttcaaagaa ggggaaaacc cgtatgcgaa caagcgtaac
    11161 accctgacgc caacccagat gcgtaaacgt aagcgtctga tgaagcacat caagaaaaat
    11221 aaataattat ccgtaagtat tttatctgcc attccctcta tgaatgtaga gggaatggct
    11281 accggaggaa ttcatatgga actgcattgt ccaaaatgtc agcatgtgct tgatcaggat
    11341 aacggccatg cgcgttgccc atcctgcggg gaatttattg aaatgaaagc tctttgtcca
    11401 gactgccatc aaccgttgca ggtgttaaaa gcctgcggtg cagtagatta cttctgtcag
    11461 cacgggcatg gcttgatatc gaaaaaacgc gttgaattcg tgctggctta atgaaccttt
    11521 ttacgcgatt tttttaccgg ctggatgttt tttacttcac tttctatcca gccgtcttcc
    11581 agacgtgtgg ttagcatttc acccgtttta acttgcttca cttttttcag tacattgccg
    11641 tcagtagcag tagtaacgct gtatccacgc gccagcgttg acagtgggct tacggcttcg
    11701 aggtgcgtta ctgcattacc gaaacgttca cgcgtggcgc taagctgtgc gcgcaggatt
    11761 tctgctaaac gatattccag ttgctgaatg cgcgtttgcg tgcgatgaat cttcggctgt
    11821 ggattttgct gattcagccg ctgtgttaat cgctgctgct gttgcccggc acgcttaagc
    11881 tgattttcca gcgcaaagct catccgtttt tgcaggcgtt caagcatggt ttgctggcgt
    11941 gccagccgga gctgcggatg ctgctgctgc aagcgatgat gaatctgcgt aaagcgacgt
    12001 gtacggttgg cgagataata atccatcgcc atctccagcc gttgacgggc cgattgcacc
    12061 tggcgcagta actcttgctg attacggctc actacttcag cggcggcaga cggcgttggc
    12121 gcacgcagat cggcaacaaa atcggcaatg gtcacatccg tctcatgccc gacggcgctg
    12181 acgaccggaa tgcggctggc aaaaatcgcc cgcgctacgc gttcgtcgtt aaaactccat
    12241 aaatcttcca gcgaaccgcc gccgcgccca acgatcaaca cgtcgcactc attgcgctga
    12301 ttcgccagtt caatggcgcg aacaatttgc cccggcgcgt catcgccctg aacggaggtg
    12361 gggtagatga tcaccggtag agaaggatcg cgacgtttta acacatgcaa aatatcatgt
    12421 agcgcagcac cggtttttga ggtgatcaca ccaacgcaat gcgcagggga gggaagtggt
    12481 tttttgtatt gctgatcgaa caaacattca gcctgcaact tcgctttgag ctgttcgtac
    12541 ttctgttgca gcagcccttc accggccggc tgcatactct caacgattat ctggtagtcg
    12601 ccgcgcggct cgtagagcgt aatattggcg cgaactaaaa cttgttgccc atgctgcgga
    12661 cggaaggtga cccggcggtt gctgttgcgg aacatcgcgc agcgtacctg ggcggtgtcg
    12721 tctttgagtg taaagtacca gtgaccggaa gccggttgtg tgaaattaga gatttcgccg
    12781 ctgatccaaa cctgtcccat ctcatgctca agcagcagac gaaccgtttg attcaggcga
    12841 ctaacggtaa aaattgcagg ggattgagaa ggtaacatgt gagcgagatc aaattctaaa
    12901 tcagcaggtt attcagtcta tagtaacccg cccttcgggg atagcaagca ttttttgcaa
    12961 aaaggggtag atgcaatcgg ttacgctctg tataatgccg cggcaatatt tattaaccac
    13021 tctggtcgag atattgccca tgctacgtat cgctaaagaa gctctgacgt ttgacgacgt
    13081 tctcctcgtt cctgctcatt ctaccgttct gccgaatact gctgacctca gcacccagct
    13141 gacgaaaact attcgtctga atatccctat gctttccgca gcaatggata ccgtaacgga
    13201 agcgcgcctg gctattgctc tggctcagga aggcggtatc ggctttatcc acaaaaacat
    13261 gtccattgaa cgccaggcag aagaagttcg ccgtgtgaaa aaacacgaat ctggtgtggt
    13321 gactgatccg cagactgttc tgccaaccac gacgctgcgc gaagtgaaag aactgaccga
    13381 gcgtaacggt tttgcgggct atccggtcgt taccgaagaa aacgaactgg tgggtattat
    13441 caccggtcgt gacgtgcgtt ttgttaccga cctgaaccag ccggttagcg tttacatgac
    13501 gccgaaagag cgtctggtca ccgtgcgtga aggtgaagcc cgtgaagtgg tgctggcaaa
    13561 aatgcacgaa aaacgcgttg aaaaagcgct ggtggttgat gacgaattcc acctgatcgg
    13621 catgatcacc gtgaaagact tccagaaagc ggaacgtaaa ccgaacgctt gtaaagacga
    13681 gcaaggccgt ctgcgtgttg gtgctgcagt tggcgcaggt gcgggtaacg aagagcgtgt
    13741 tgacgcgctg gttgccgctg gcgttgacgt tctgctgatc gactcctccc acggtcactc
    13801 agaaggcgtt ttgcagcgta tccgtgaaac ccgtgctaaa tatccggatc tgcaaatcat
    13861 cggcggcaac gtggcaacag ctgcaggtgc acgcgctttg gcagaagctg gttgcagcgc
    13921 ggttaaagtc ggtatcggcc ctggttctat ctgtacgact cgtatcgtta ctggcgtagg
    13981 tgttccgcag atcactgccg ttgctgacgc agttgaagcc ctggaaggca ccggtattcc
    14041 ggttatcgca gatggtggta ttcgcttctc cggcgacatc gccaaagcta tcgccgctgg
    14101 cgcaagcgcg gtgatggtag gttccatgct ggcgggtact gaagaatctc cgggtgaaat
    14161 cgaactctac cagggccgtt cttataaatc ttaccgtggt atgggttccc tgggcgcgat
    14221 gtccaaaggt tcctctgacc gttatttcca gagcgataac gctgccgaca aactggtgcc
    14281 ggaaggtatc gaaggtcgcg tagcttataa aggtcgcctg aaagagatca ttcaccagca
    14341 gatgggcggc ctgcgctcct gtatgggtct gaccggctgt ggtactatcg acgaactgcg
    14401 tactaaagcg gagtttgtac gtatcagcgg tgcgggcatt caggaaagcc acgttcacga
    14461 cgtgaccatt actaaagagt ccccgaacta ccgtctgggc tcctgattct cttcgcccga
    14521 tttcatgtcg ggcgatttat attatctgtt tcacttgcct cggaataagc gtcaatgacg
    14581 gaaaacattc ataagcatcg catcctcatt ctggacttcg gttctcagta cactcaactg
    14641 gttgcgcgcc gcgtgcgtga gctgggtgtt tactgcgaac tgtgggcgtg ggatgtgaca
    14701 gaagcacaaa ttcgtgactt caatccaagc ggaattattc tttccggcgg cccggaaagt
    14761 actactgaag aaaacagtcc gcgtgcgccg cagtatgtct ttgaagcagg cgtaccgata
    14821 ttcggcgttt gctatggcat gcagaccatg gcaatgcagt tgggcggtca cgttgaagcc
    14881 tctaacgaac gtgaatttgg ctacgcgcag gtagaagtcg taaacgacag cgcactggtt
    14941 cgcggtatcg aagatgcgct gaccgcagac ggtaaaccgc tgctcgatgt ctggatgagc
    15001 cacggcgata aagttaccgc tatcccgtcc gacttcgtca ccgtagccag caccgaaagc
    15061 tgcccgtttg ccattatggc taacgaagaa aaacgcttct atggcgtaca gttccacccg
    15121 gaagtgactc acacccgcca gggtatgcgc atgctggagc gttttgtgcg tgatatctgc
    15181 cagtgtgaag ccctgtggac gccagcgaaa attatcgacg atgctgtagc tcgcatccgc
    15241 gagcaggtag gcgacgataa agtcatcctc ggcctctctg gtggtgtgga ttcctccgta
    15301 accgcaatgc tgctgcaccg tgctatcggt aaaaacctga cttgcgtatt cgtcgacaac
    15361 ggcctgctgc gtctcaacga agcagagcag gttctggata tgtttggcga tcactttggt
    15421 cttaacattg ttcacgtacc ggcagaagat cgcttcctgt cagcgctggc tggcgaaaac
    15481 gatccggaag caaaacgtaa aatcatcggg cgcgttttcg ttgaagtatt cgatgaagaa
    15541 gcgctgaaac tggaagacgt gaagtggctg gcgcagggca ccatctaccc tgacgttatc
    15601 gaatctgcgg cttctgcaac cggtaaagca cacgtcatca aatctcacca caacgtgggc
    15661 ggcctgccga aagagatgaa gatgggcctg gttgaaccgc tgaaagagct gttcaaagac
    15721 gaagtgcgta agattggtct ggagctgggc ctgccgtacg acatgctgta ccgtcatccg
    15781 ttcccgggac caggccttgg cgttcgtgtt ctgggtgaag tgaagaaaga gtactgtgac
    15841 ctgctgcgcc gtgctgacgc catcttcatt gaagaactgc gtaaagcgga cctgtacgac
    15901 aaagtcagcc aggcgttcac cgtcttcctg ccagtacgtt ccgttggcgt aatgggcgat
    15961 ggtcgtaagt atgactgggt tgtctctctg cgtgctgtcg aaaccatcga ctttatgacc
    16021 gcacactggg cgcatctgcc gtacgatttc ctcggtcgcg tttccaaccg cattatcaat
    16081 gaagtgaacg gtatttcccg cgtggtgtat gacatcagcg gcaagccgcc agctaccatt
    16141 gagtgggaat gattttgatc cctgcactat gaatgaacaa aaccctccgt tactacagag
    16201 ggttttttat cttcaagaat cataggcttg aagttactaa catcgattaa ttaaaccagc
    16261 tgtccgattt gttctcttct gctttgccca cgcttttcat cagatcgcga ccgccttcag
    16321 tcatatttct gtttgcgtca gcttcagatt gcaccacatc ggtttgcgca gctttgtgct
    16381 tcagttcctg atcgataaat tcgttttcgc gcttaacgcg ggcttcttct ttcgccagcg
    16441 ccagtttttg tttctgaatc tctaagctgc gtagctcatc ttcataactt tgatcgcgtt
    16501 ttttgtccgc agtggcttcg gcgtccagtt tatcctgacg agctttctta tttgccgctg
    16561 ccgttgccgc tcttttgttc gcggcggcct gggcatttgc gcgacgttgc ttctcttgct
    16621 ggatttccct gttgcgctcc gcgacccatt cgtcatgctg cctttgctct tcatttttac
    16681 cttgctgttc cgcttctgcg acagcagaga gttgatcctg caatgatgag gctatagccg
    16741 gatagcttaa ggaggctaag atggcgcaaa gaaaaacttt cttcatgact cctcctgatt
    16801 attagctctt ttcaggacat ttggtatttg gctgaatacg cgtttcgtta tacgttgtgg
    16861 taataacaac ggctaaacct gtcgtaaact ggcactcttt accctcctgg gtggaggtat
    16921 acactttggt gccttcctta tatgttaaag aaacaccttc cactaaggtt ttatcattca
    16981 ccatagaacc cgctgccgcg cctacagctc cgccgccaac tgcccctgcc gtcgttccgg
    17041 aattgctgcc agaaccgacg ttgtggccga taacaccgcc agcgactgcg ccaataagcg
    17101 cgccgaaggc ttgtgcgttc cgtttatttt gggagttgtc tacggcaact tttgcgggaa
    17161 gaatggaaat aatattaacg gttttagttt cttgtttggt attcagttga tcggtttgat
    17221 aaacatcggc ggcatgatcg tcagcatttg actggcatcc tgccagagtg aatgacgcta
    17281 acattgccac aggcagaaga cattttttaa atttcatcac tattccttgt tatacacatt
    17341 acgaaaaata agacatttaa gatcatc
//
LOCUS       DAZPAB010000077        17144 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZPAB010000077 DAZPAB010000000
VERSION     DAZPAB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.077"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..510
                     /locus_tag="ACHUPG_002218"
     CDS             <1..510
                     /locus_tag="ACHUPG_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR0861825.1"
                     /translation="PFREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDG
                     FTQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATI
                     RHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADR
                     QYSRAWTVK"
     gene            complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="ACHUPG_002219"
     CDS             complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="ACHUPG_002219"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR0861826.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1780..2868
                     /gene="hcxA"
                     /locus_tag="ACHUPG_002220"
     CDS             1780..2868
                     /gene="hcxA"
                     /locus_tag="ACHUPG_002220"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR0861827.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2878..3075)
                     /locus_tag="ACHUPG_002221"
     CDS             complement(2878..3075)
                     /locus_tag="ACHUPG_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR0861828.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="ACHUPG_002222"
     CDS             complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="ACHUPG_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR0861829.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5543..5956)
                     /gene="entH"
                     /locus_tag="ACHUPG_002223"
     CDS             complement(5543..5956)
                     /gene="entH"
                     /locus_tag="ACHUPG_002223"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR0861830.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5959..6705)
                     /gene="entA"
                     /locus_tag="ACHUPG_002224"
     CDS             complement(5959..6705)
                     /gene="entA"
                     /locus_tag="ACHUPG_002224"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR0861831.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6705..7562)
                     /gene="entB"
                     /locus_tag="ACHUPG_002225"
     CDS             complement(6705..7562)
                     /gene="entB"
                     /locus_tag="ACHUPG_002225"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR0861832.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7576..9186)
                     /gene="entE"
                     /locus_tag="ACHUPG_002226"
     CDS             complement(7576..9186)
                     /gene="entE"
                     /locus_tag="ACHUPG_002226"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR0861833.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9196..10371)
                     /gene="entC"
                     /locus_tag="ACHUPG_002227"
                     /pseudo
     CDS             complement(9196..10371)
                     /gene="entC"
                     /locus_tag="ACHUPG_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10663..11619
                     /gene="fepB"
                     /locus_tag="ACHUPG_002228"
     CDS             10663..11619
                     /gene="fepB"
                     /locus_tag="ACHUPG_002228"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR0861834.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11623..12855)
                     /gene="entS"
                     /locus_tag="ACHUPG_002229"
     CDS             complement(11623..12855)
                     /gene="entS"
                     /locus_tag="ACHUPG_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR0861835.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12966..13970
                     /gene="fepD"
                     /locus_tag="ACHUPG_002230"
     CDS             12966..13970
                     /gene="fepD"
                     /locus_tag="ACHUPG_002230"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR0861836.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13967..14959
                     /gene="fepG"
                     /locus_tag="ACHUPG_002231"
     CDS             13967..14959
                     /gene="fepG"
                     /locus_tag="ACHUPG_002231"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR0861837.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14956..15771
                     /gene="fepC"
                     /locus_tag="ACHUPG_002232"
     CDS             14956..15771
                     /gene="fepC"
                     /locus_tag="ACHUPG_002232"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR0861838.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15768..16901)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUPG_002233"
                     /pseudo
     CDS             complement(15768..16901)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUPG_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
BASE COUNT         4007 a         4874 c         4453 g         3810 t
ORIGIN      
        1 ccctttcgtg aaaagccagt ggattgcgtt ctgtgggtta aaaacagcca acttatgcct
       61 aatgattaca acccaaacaa cgtggcacca cccgaaaaaa aactgctgca aaaatccatt
      121 gaaatagatg gttttaccca acccattgtg gtcacacata cggataaaaa tgccatggaa
      181 atcgtggatg gttttcaccg ccatgaaatt ggtaaaggct catcatcatt aaaattacgc
      241 ttaaaaggtt atttgccggt gacctgtctg gaaggtacgc gtaatcaacg catcgccgcg
      301 actattcgtc ataatcgcgc tcgtggacgc catcaaatca ccgccatgtc ggaaatcgtc
      361 cgtgaactta gccagttggg atgggatgat aataaaattg gcaaagagct gggaatggac
      421 agcgacgaag tgctgcgact gaagcaaatt aacggcctgc aagagttgtt tgccgaccgt
      481 caatattccc gcgcctggac ggttaaatag ctaaagctgg cgcaggcgtt cagctgctgc
      541 cagcaacgtc gattccttct tggcaaaaca gagacgaatc agtttatgtg ggaagggatc
      601 ggcgcaaaac accgacagcg gaatcgccgc tacgccgtgc tcctgcgtca gccactggca
      661 aaactcaaca tcatccaggg tagaaaccgc gctgtaatcc accagcaaaa agtatgtacc
      721 ttcacacggt aaaatctcca gccggctttc atttaaagca ttcaccagaa tatcgcgctt
      781 ctggcgataa aagtccggta acgcaagata atgctcaggt tctgcacgta gcatatcagc
      841 aagcgccagc tgtgccgggg tattcaccga aaaggtcaga tactgatgta ccttgcgaat
      901 ttcggcgctg atgggcgctg gcgcaacaca ataacccact ttccagccgg tcatatgata
      961 ggtcttgcca aatgaagaaa ccgccactgc ccgctcacgc agctgcggat gcgccagcac
     1021 actggcatgg ccctgttgtg aaaagttgat gtgctcgtag acttcatcgc taatgacaaa
     1081 aatctcgtgc ccggcgatcg cctgccacaa agcggcgaaa tcagcctgct gccagacagt
     1141 tgcactgggg ttatgcggag tgttgaggat caccagtctg gtgcgctcgc ttaataatgc
     1201 ggcaaattcc tgccagtcaa cgcgaaaatg cggtggttgc agtgccatac gtttcactat
     1261 tcccccagaa agcgcgatgg cgggggcgta actgtcatag ctgggatcaa aacaaatcac
     1321 ttcatcgcca ttgcgcacca gtgcggtaat cgccgcgtat aacgcttccg tcgcccctgc
     1381 cgttacggtg atatcgctat cggcatctgg ttgatagcca tacaaacgtt ccgttttctg
     1441 agcaatcgcc tcgcgcaagg cctgcacgcc ggtcatgggc gcgtattggt ttgccccctg
     1501 ggcaacgtgg tacgccagcc gctcctgtaa atagcgcgga ccatcaaaat caggaaagcc
     1561 ttgcgacagg ttaatcgcct ggtgttgctg cgccagcgcg ctcatctggg tgaaaatagt
     1621 ggtgccaagt tgtggaagtt tgctttgtgg aatcagaggg ttatttgtca tttttattgt
     1681 acctgacgaa gttgcgtgtt gaaggcacta taacacgatg ccagtatttg gcaatcaaga
     1741 cgtttagatg tctaaataaa acaataagga caacacaaca tgcctcacaa tcctatccgc
     1801 gtggtcgtcg gcccggctaa ctacttttca catccaggaa gtttcaatca cctgcacaat
     1861 tttttcactg atgaacaact ttctcgcgcg gtgtggatct acggcaaacg cgccattgct
     1921 gcggcgcaaa ccaaacttcc gccagcgttt ggacttccag gggcaaagca tattttgttt
     1981 cgtggtcatt gcagcgaaag cgatgtacag caactggcgg ctgagtccgg tgacgatcgc
     2041 agcgtggtga ttggcgtcgg cggcggcgca ctgctcgaca ccgcgaaagc cctcgcccgc
     2101 cgtctcggtc tgccatttgt tgccgctccg acgatcgccg ccacctgcgc agcctggaca
     2161 ccgctctccg tctggtataa cgatgccgga caggcgctgc attatgagat tttcgacgac
     2221 gccaatttta tggtgctggt ggaaccggag attatcctca atgcaccgca acaatatctg
     2281 ctggcgggga tcggtgacac gctggcgaaa tggtatgaag cggtggtgct ggctccgcaa
     2341 ccagaaacgt tgccgctaac cgtgcggctg gggatcaata atgcgcaggc cattcgcgac
     2401 gtcttgttaa acagtagcga acaggcactt gccgatcagc aaaatcagca gttaacgcaa
     2461 tcattttgcg atgtggtgga tgctattatt gctggtggtg ggatggtcgg tggtctgggc
     2521 gatcgtttta cgcgtgtggc ggcggctcat gccgtgcata acggtctgac tgtcctgccg
     2581 caaaccgaga agtttcttca cggcaccaaa gtcgcttacg gaattctggt gcaaagcgcc
     2641 ttgctgggtc aggatgatgt gctggcgcaa ttaaccgggg cgtatcagcg ttttcatctg
     2701 ccgactacgc tggcggagct ggaggtggat atcaataatc aggcggagat cgacaaagtg
     2761 attgcccaca ccttgcgccc ggtggagtcc attcattacc tgccagtcac gctgacacca
     2821 gatacgttgc gtgcagcgtt cgaaaaagtg gaatcgttta aagcctgacg tgcaaattta
     2881 gcagcagcgc gcgccgcctt ttccaccgta gcgcgcgtcc tgccgctcac ggaaaaactc
     2941 ctcgtaggtc atcggcgttt gatcgggatg attaacccgc atatgttcga catagttgtc
     3001 gtaatcaggc ataccaatca tcagcttcgc cgcctgacct aaatattttc cggcttttgc
     3061 cagtgaatca aacatcttgg cctccgctaa ccatgttgct gaaagagtgc cggatgcggc
     3121 gtgaacgctt tatccggcct acaaatgcta tcaacgcact cggtcagtcc cctcgcccca
     3181 tcggggagag ggttagggtg agggggaacc ctcggcacta tgccaacatt cgccaacatc
     3241 ccccctcact ctgactttag tgcgcgcctt tcgcctgcgc tacgatctcc tcgacatttt
     3301 ccggcatcgg ctcatacggc gtttctttcg ccgttggctt cggatctttc aatgccgcaa
     3361 gtgccgtctt aatcgagaac agtgccagga ccaccacgac caccataaag aagatggtta
     3421 acccggcatc cagacggttg ttgaacacca gttgcgccag ctgtgactca gtatactgcg
     3481 acggaatatt gccgctgtcg atcattgcct ggaacttgtt agcaatgacc aggaagccga
     3541 ctttcgcatc cgggctaaac gctttcagcc agtcagcggt cagggtacaa atcagcagcc
     3601 aggccgttgg taccagcgcc acccaggcgt aacgttgacg tttcatcttg aacaacacca
     3661 cggcacagag catcagcgcc atccctgcca gcatctggtt ggcaatacca aacagcggcc
     3721 acagagtgtt aataccgccc agcggatcga ccacgctctg atggaggaag tagccccacg
     3781 ccagtacgca cagcgctgtt gccagcaggt tagcaggcag tgaatcggtc cgcttcaggc
     3841 caggagacac cacgcccagc agatcctgca acataaagcg cgcagcacgc gtacccgcat
     3901 ctaccgccgt cagaataaac agagcttcaa acagaatggc gaagtgatac cagaacgcca
     3961 catccatcat accacccagc gcaccgtgca ggatgtaagc catccccacc gccaacgtcg
     4021 gcgcaccgcc cgcacgggaa atgatcgact gttcacccac ttcgctggca atctggttga
     4081 gcgtatctgg tgtaatgcta aagccccagc tactcaccac ctgcgcggca gaagcgacta
     4141 catccgccgt ccctgccgga gccagcaccg ccattgggct gttcatggca aaatacacgc
     4201 ccggatcgat gatacaggca gaaactagcg ccataatcgc cacgaaggat tccatcaaca
     4261 tcccaccgta gccgataaag cacgcctgcc cttcgttcgc cagcatcttc ggcgtggtgc
     4321 cggaagagat cagcgcatgg aagccagaca ccgcaccaca ggcgatggtg ataaacagga
     4381 acgggaacag gttaccggtc cataccgggc cagtgccatc gacaaatttg gtcagcgcag
     4441 gcatggtcag cgtcgggcgc ataatcaaaa tgcctaccgc cagaccgacg atagtcccga
     4501 ttttcaggaa ggtcgagaga tagtcacgcg gggccagcag caaccacacc ggcagcaccg
     4561 ccgccacaaa accgtagccc accagcatcc aggtcagctg cacgccggta aagtcaaagt
     4621 acggtgccca ggtcggactt tctgccaccc agccgccaga gataatggcg aaaatcagga
     4681 acaccaggcc aatcactgac acttcgccaa tgcggcccgg acgcagatag cgcaggtaga
     4741 ttcccataaa aatcgccagc ggaatggtga acgcaacggt atacgttccc cacgggctat
     4801 gagtcagggc tttcaccacg atcatcgcca gtactgcaag gataatgacc atgatcataa
     4861 agcaggctac cagcgcaatc accccggcag ttggccccat ctcttctttg accagctcac
     4921 ccagcgagcg accgtcacgg cgcgtagaaa caaacagcac catgaaatcc tgcaccgcac
     4981 cggcgagaac caccccggcg agcagccaga tcatccccgg caggtagccc atttgcgctg
     5041 ccagcaccgg ccccaccagc ggccctgctc cggcaatggc cgcaaaatgg tgaccgaaca
     5101 gcactttctt gtccgtcggc acatagtcca gcccgtcgtt atggcgcacc gctggcgtca
     5161 tacgcgtcgg gtcaaccgcc agcacatttt tggcgatata cagcccataa aaacggtaag
     5221 cgatcagata gatacagacc gacgccacca caatccacag cgcgttgatc tgttccccac
     5281 gatttaaagc aatgtatccc agagcaaatg ctcccattac agagagcact gtccagacga
     5341 ggtatttccc tgatttgttc atagttgtta tccgtgtgcg tgtccataga gatgttacat
     5401 tttgtttcta taacatcttt gtacgattta acaacacggt aaccatgtaa atcggagttg
     5461 aaaccaatat ttaacttaac aatgttaact cgatcactcc gttaaccgag ttttcctgca
     5521 atctcattta attctgtcgg gctcatccca aaactgccgt acccagccga caagtgcagc
     5581 aacgccgccc ctgttcatcg aaaacgacga tttcccaact ctgattttgc ctcccaagat
     5641 gcagcggctg gcagacgccg cgtaccttgc cttcagacac cgggcgatga tgggtggcgt
     5701 taagttccgt gcccaccaca cactgcccgt cacgggtcat cataaatccg gccatcgatc
     5761 ccagcgtttc agccagcgcc gccgacgcgc cgccatgcag caggccaaac ggctgatgag
     5821 tacgggtatc aaccggcatt tcggcttcca gcacatcatc tcccagaagg gtatacacaa
     5881 ttcccagatg cgccaccatt gtgttatcgc tggtggcgtt cagttcgtcg agcgttaaat
     5941 ggcgtttcca gatcatgctt atgcccccag cgttgagccg ccatcgacca caatatcctg
     6001 tagggtgata tggctggcga ggtcagaggc gaggaacaaa atcgtgttgg cgatctcttg
     6061 tggacgggcg attttcccca gcggaatgcc gagtttaaac tgctcgccaa agccgcgaat
     6121 acgctgttct tcggcgtcat cgctcaccca cagcgtgcgt tgcatatcgg tgtcggtgga
     6181 gccaggcgaa accacattac agcgcacgcc gctacccgcc agttccagcc cgacgctcaa
     6241 cgccaggctt ttcagcgccg ctttcgatgc gccataagca ctcataccaa tacgcgccgt
     6301 gtgtgcggcg tcggacgcca cagtgacaat cgccccgccc cgctgacggc gaaactggtt
     6361 catggtttgc tggaacaggt taaacgcacc gccgacgtta accgcaaaag tctgctgcca
     6421 gtcctcttta ctgagctgat cggtcgcgcc catgcgcaaa attcccgccg cattgatcag
     6481 cacgtccagt cgctccgttt cagctaacag tcgctgacat acctgcgcca cctgcccggc
     6541 gtcggcaaca tccattactt cggtcgcaaa gggatattgc tcctgagtga acgcttgatc
     6601 aaaacctgta actttcgctc ccgcctcaac aaacgtcagc gccgtggcgt agccgatacc
     6661 tttacctgcg ccggttaccc agacattttt accgctgaaa tccattattt cacctcgcgg
     6721 gagagtagct tccaccaggc gtcgatggtc gggtttttcg ccagcatgac aaagtcgata
     6781 tcaccatgca ctttgcgcca gcgcgccgcc agcgccatca tgcgcaccga atccagaccg
     6841 tagtcgatca ggttgtcgtc atcgaacggt tcatcggact cgtccagcaa cggcaggatc
     6901 acctcacgca gcgccgcttt gctggcgggg ataggtgctg gcagtaattc ttcggtcatc
     6961 accacccggc cagaacgtcc ggccacatat ttcagcgaca tcaaatgctc gtcacggctg
     7021 aaatcagcca gcgcatccgc caccataaac ggtttaatat cgcgcataaa tgcgtcggtt
     7081 gcggtggtca tacagccaat gtgggcatat actcctgtaa taatcagctg gttacgtcca
     7141 ctctctttga gcatttgctc cagcggagaa cgatgaaacg cgctgtagcg ccacttcacc
     7201 agcaccgtgt cgtcggcatc tggcgtcagg cgatccacca ccttttgctg ttccggcgag
     7261 cgggtcaggc ccggccccca catatcattc aacagcgccc gatcttcatc gctctgctct
     7321 ttcggctggg cggtgtaata aaccgggata ttgtgctgtt tgcagtagtc gcgcagcgca
     7381 gcaatattcg cgatcacctg ctccatcatc gggcagttct cgccccagaa gctgacaaaa
     7441 tagtcctgca tatcatggat taacaacgcg gcacgttgcg gttcaaaggc ccagtcaact
     7501 ttattctgcg gaatatcgtg agactccggc agtgcgtaag cctgtaattt tggaatagcc
     7561 atcgtgttct ctccttcagg ctgatgcgcg tgacgccagc cactgacgta attgtttttt
     7621 atcgactttc ccgaccgccg tcagcggaag tgaatccaca cactccacgc gatccggtaa
     7681 tttaaattcg gcaataccct gttcacgcag gaaacgacgc acctgcaccg cgcgcagcgg
     7741 ctcttttacc accagataag cacagctttt ttcgcccatc agctcatttt ccatgctcac
     7801 cagtgcggca tagatcactg ccggatggct cagcagcagg ttttcgatat cttcggcagc
     7861 gatcttctcg ccgccacggt tgatctgatc tttctcgcgc ccctgcacgg tgatgtaacc
     7921 ctctggatca atagagatca gatcgccgga acagtaaaaa ccgttggcat caaaggcact
     7981 ggcattgtgc tgcggacttt tgtaatagcc acggaaggtg tacggcccgc gcgtcatcag
     8041 gcgtccaact tccccttgcg gcagtggatt tccttcggca tcggcaaccc acacttcatc
     8101 gtccggacac attgggtaac cctgggtatg gataattttc tccgcgctat catcaagacg
     8161 ggtgtagttc accagccctt ccgccatgcc aaacacctgc tgcaactggc agccaatctc
     8221 agcgggaata cgcgctgcaa gcgtggcaga aagacgtgcg ccgccgacct gtaacagttt
     8281 cagcgaggca agctgcgccc ggctttcgcc ttcagccagc gcctgcaacc acaggctgac
     8341 tgccggcggc accagcgcgg taacgttaac ctgatgtttt gcaatcaacg ggaagcaaag
     8401 cgtggcgctg ggatcggccg ccagaacaac cgttcctccg gcaagaaaga cgcccagcga
     8461 tcctggcgaa ctcatagtgt agttatgagc cgccgggatc gagcacaggt agcgcgtctg
     8521 ttgtgtgaac tgacaaatct cggcgctacg acgcacgctg tagtaatagt cgttatgagt
     8581 gcgcgggatc agtttcggtg tgccggtggt gccgccggaa agctggaaat aggccacttc
     8641 atcagcaggt gatggcgtgg cagtaaaacc ctctgccgga tggttaatcg catcctgcaa
     8701 gttatgctca ccgctgtcgt tgtgcagttg caccacgcga atggaagaat gctctgcaac
     8761 aaatgtgttg aggaaatcat ccccgctaaa tagcgcatgc tggcgatcgg caatcagtaa
     8821 cgccggttca atctggctgg cgtaggcgtt cagttcacta cgctgatggc tgaacagcgc
     8881 cagcaccggc gcgacgccca gtttcagcag cgcgaaaaag gtaatgtaaa gttcagcgac
     8941 gttacccagt tgtaccagcg cggtttcacc aggtttaatg ccctgacggc gcaaactaca
     9001 ggcgaggtta tccgccgcct gattcagctc ccgataactc aactgccgct cgccgtcgat
     9061 aaccgcgatg ctgtcactcg cagcgtggcg agtcagaatg tcggtcagtg gcaaatcctg
     9121 ccagtagcct ttttcccgat agcgacgggc aaactcttcc ggccagcggg tgaatggaat
     9181 gctcatcctc gctccttaat gcaatccaaa aacgttcaac atggtagaaa gtttgacgcc
     9241 tgtttcgcgc cactcaccca acggtgacga cgcaggcaca atccccgctc cggcaaacag
     9301 acgcacctga ttttcccgca gcttcgcgca gcggatggtc accacccatt cgccgttacc
     9361 ttcgctgtca caccaaccca caatgccgcc aaacagttcg cggtcgaacg gctccagttc
     9421 agcaataacc tgggtcgcgg cctgatgcgg gaaaccgctc agcgcggggg tcggatgcag
     9481 cagacaggcc agagtcagtg cgttttcttg cgaattcgct ttaccttcaa agggagttgc
     9541 gagatgccac agcgtcggcg tggtaatcaa ttgtggagag gagggaacgt gtaactcact
     9601 actgcgttcg cgcagtacct ctttcatcgc ctgagtcccc agttcatgtt catggcgatc
     9661 tttttctgac gccagcagac gattacccgc ttcgcgatcg agcacttcat ccggctgacg
     9721 acgcgcggaa ccggctaacg gaatggagct aaaacgctcg ccgtctttac gtagcagcag
     9781 ttccgggctg gcccccagca ggacgccacc atcagccagc gggacatgga agttgtaact
     9841 aaccgggttt tgcgcaatca accgttccag caatacgcca ctatcaatgg cggcgtcagt
     9901 ggtgatatca atcaatcgtg acaacaccac tttgtcgacc tgcggcgtgg cggtaagtgc
     9961 ggcggcgcgg gcacccatct gttcaaacgt ggtttgttca ggaattgcct agcgttccac
    10021 cacgttcagc gactggctgc gggtaaaaca gcgggctgag gtctgttttt cctgacggga
    10081 gaacgactgc taggattcgg gaatatacag cgacgaaggc tgacgtggat cgaagggaat
    10141 cgccccgacc atcaccggat ttttgatgcc ctgcgctttg gcatcggcaa acagcgcggc
    10201 gagtttttgc tggaagggac tgtcgggcga atccccgttc acagccggtt catcgaaacg
    10261 ggcgaaacat cctgacgtcg taaaactgcg gtacggcgac ataaagaaaa agcgattggg
    10321 cgcaagtgtt gccatggtct gctgtacttc ctcagccagt gacgtatcca tatcatcctc
    10381 cacaaaatga taaaggcttt aataatgatt atcacttata ttttcggccg ctaacctaaa
    10441 agcaaacgcg cactatgtca actcttgagg taacgcaatc tgcaactcgg ggttgcatct
    10501 ggttgtggtt acaatgaaaa tgagaagcat tattgatgaa ttcgcataag cgcaatgtga
    10561 tgtcctgcgc cgttttgccc cctctcccca aagggacgga ttgtgcgctt tgtcgaattt
    10621 gtcattacgc ccttaacctt attaataaca ggaagctgac ttgtgagact cgccccgctc
    10681 taccgcaacg cccttctatt aacaggactt ttgctttcag gaatagccgt agttcaggcc
    10741 gccgactggc cgcgtcagat tactgacagc cggggcactc ataccctgga aagccagccg
    10801 cagcgtattg tttccaccag cgtcaccctg accggatcac tgctggcgat tgatgctccg
    10861 gtgatcgcca gcggcgcgac cacgccgaat aaccgcgtcg cagatgacca gggcttttta
    10921 cgccagtgga gcaaggtggc gaaagaacgc aaactgcaac ggctctatat cggcgaaccg
    10981 agcgccgaag ccgttgccgc gcaaatgccg gatctgattt taattagcgc aaccggcggg
    11041 gattcggcgc tggcgctgta tgatcagctt tccaccatcg ccccgacatt aatcatcaat
    11101 tacgacgaca aaagctggca gtcgctgtta acgcagcttg gcgaaattac cgggcatgag
    11161 aaacaagcgg cagagcggat tgcacagttt gataagcaac tggcagcggc gaaagagcaa
    11221 atcaaattac cgccgcagcc ggtcactgcc attgtctata ccgccgctgc acacagtgcc
    11281 aatctctgga cgccggaatc agcacaaggg cagatgctgg aacaactcgg ctttacgctg
    11341 gcgaaattgc ccgcagggtt aaacgccagc caaagccagg gcaaacgcca tgacatcatt
    11401 cagcttggtg gggaaaatct ggctgcgggg ttaaacggtg aatcgctatt cctgttcgct
    11461 ggtgatcaga aagatgccga tgcgatttat gccaatccac tgctcgcgca cctgcctgcg
    11521 gtacaaaaca agcaggttta tgcgctggga accgagacgt tccgcctgga ttactacagc
    11581 gccatgcaag tgctggaacg gcttaaggcg ctgttttaag cattaactgt cggacgctgt
    11641 cacctgcggc ggcgtctggc gaaaacgtcg caactccacc agcaccagca ataacaacac
    11701 gccgataatc aacaaaccaa aaccgctcgc gcttgcggaa gcaaccggtg tcatcatcgc
    11761 gcccaaacca cccagcagcg ccgcgcctat ggcatcgccc gtcacgttct gcgccgtcca
    11821 caaaccgtta atccgcccta acatcgcttc cggggtttgc gtttgcagca ttgtatattg
    11881 cagcaacgaa ctaaccgcgc tcagccagcc gaacagcgcc agacaaacca cgcctaaaat
    11941 ccacatcggc atcaggccaa acagaccaat ggcgaggaac gatcccagcg tggagagcag
    12001 catcaataac cctggtcgcg cactatgtgc cagcttcccg ctggttaacg caccaatagc
    12061 cgcgccgagc gggatcgccg cgtagagaaa accaatctgt gccgctgaca tctgccagtt
    12121 gtcagccagc gccggataca gtaccagcac cgcgctcgcc atcgtcaata aaccacccag
    12181 cagcgggctg gcaagcagaa aacgaaatcc tgccagtaat gatttcaacg gatgctcaag
    12241 cggctgcggt ggcggtggca actccggaag gcttaataac ggtaacaagg taataaacgt
    12301 gcccgccgcc gccagcccgt agttccaggc tacgccgccg gtcgccagca ataaaccgcc
    12361 aatcatgggc gaaatcaccg accccaggcg cacggtcaac atggtgatcg ccccggcctg
    12421 cattaagttt tcacgcccta ccagtgcaga tgttgccgcc agtagcgccg taacaccaag
    12481 tgatgcgaaa aaaccatccc ataaaccaag taaatagatt gccagcaatg acggctccgg
    12541 cagcagtgca ttaaggcaca gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa
    12601 aatcactttt ttgcgttcat agcgatccgc cagcacaccg ccaaccatca ggccaacaaa
    12661 catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc caggtagaat gggtcatcat
    12721 ctggatctgc accggcaccg cgacgccgag caaacccaga gacacaattg agataaaacg
    12781 agcgaggaat actgcgcgaa acgccgggtg cgttttcaac aggctgaggt taagcagcca
    12841 ggattgttta ttcattacaa tgccttgcca tcaaatattt taaataccta ttcttaggct
    12901 gcacatgtta acatatccaa ataagatcga taacgataat taatttcatt atcatggaag
    12961 ttcgtatatc tggttctgtt gccgtgacac gcgccattgc cgtgcccgga ttgctgttat
    13021 tactgattat cgcgacggca ttaagcctgc tcattggggc aaaatcactc cccgcttccg
    13081 tagtgctgga ggccttctcc ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg
    13141 cgcggctgcc gcgtaccctt gccggtttac tggcaggcgg cgcgcttggc cttgccgggg
    13201 cgttaatgca aaccctcacc cgaaacccac ttgccgaccc cggcttgctt ggcgtgaacg
    13261 ccggagccag ctttgccatt gtgctgggtg cggcgctgtt tggttactca tccgcacagg
    13321 aacaactggc gatggccttc gccggggcgc tggtggcctc attgattgtt gcctttaccg
    13381 gcagtcaggg tggagggcag ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg
    13441 cggcggtgct ggaaggactg accagcggca tcgccctgct taatcctgac gtctacgatc
    13501 aactgcgttt ctggcaagcc ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg
    13561 tgctgatccc ggttctgatc gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca
    13621 gtttgagcct cggcagtgac accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt
    13681 tgattggtct gctggcgatt accgtgcttt gtggtagtgc gacggcggta gttggcccga
    13741 ttgcctttat cggcctgatg atgccgcaca tggcgcgctg gctggtaggt gccgatcatc
    13801 gctggtcgct gcccgtcacg ctacttgcta cccctgccct gctgctgttt gccgatatca
    13861 tcgggcgggt gattgtgccc ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg
    13921 caccggtgct gatcttcctc gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc
    13981 tcgccgatta ctcatcacct gtttgctgct gatttccgcc tgtgtggttg caggtatctg
    14041 gggattacgc agcggtgccg tcacgctgga aatctcgcag gtattcgccg cgttgatggg
    14101 cgatgcgccg cgcagtatga cgatggtggt caccgaatgg cgtttaccac gcgtgctgat
    14161 ggcgctgttg attggcgcgg cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg
    14221 taacccgctc ggcagccctg acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct
    14281 ggtggcgatg gtgctgtttg gtcaggatct gacggctatc gcgctggcag caatggtggg
    14341 cggcattatc acttcgctgt tggtctggct gctcgcctgg cgtaacggta tcgacacctt
    14401 tcggttgatt attatcggta tcggcgttcg cgccatgctg gtggccttta atacctggct
    14461 gttgctgaaa gcgtctttag aaacggcgct aacagcaggt ttgtggaatg ccggatcgct
    14521 caacggcctg acgtgggcaa aaacctcgcc ttccgcaccc atcattatat tgatgctcat
    14581 tgccgccacc ttgctggtgc ggcgaatgcg cttgctggag atgggcgatg ataccgcctg
    14641 cgcgctgggc gtcagcgtcg agcgttcgcg tctgttaatg atgctggttg cagtggtgct
    14701 taccgctgcg gcaacggcgc ttgccgggcc gatttccttt attgctttag tcgcgccgca
    14761 cattgcccga cgcattagcg gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg
    14821 agcgctgtta ctgctggcgg ccgttctctg cgcccaacaa ctgtttatgc cgtatcaact
    14881 tccggttggc gtcgttaccg tcagcctcgg cggtatttac cttatcgtct tgttaattca
    14941 ggagtctcgc aaaaaatgac cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg
    15001 ggatatggca aatataccgt tgcggaaaat ctgactgtag aaattcctga tggtcacttc
    15061 acggcaatta tcggaccaaa tggctgtggt aaatccacgt tactgcgtac cttaagccgc
    15121 ctgatgacgc ctgctcacgg gcatgtctgg ctggatggcg agcacattca acattacgcc
    15181 agtaaagaag ttgcacgccg gattggtctg ttggcgcaaa atgctaccac gccgggcgat
    15241 atcaccgtgc aggagctggt ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc
    15301 tggcgcaaag aggatgaaga agcggtaacg aaagcgatgc aggccacggg aataactcat
    15361 ctggcagatc aaagcgtgga taccctttct ggcggacaac gccagcgagc gtggatcgcg
    15421 atggtgctgg cccaggaaac ggcaattatg ctgctcgacg aacccacgac ctggctggat
    15481 atcagtcatc agattgatct actggaactg ttaagcgaac tgaaccgcga gaaaggctat
    15541 accctggcgg cggtgctgca cgatcttaat caggcctgtc gttacgccag ccatttgatt
    15601 gcattgcggg aagggaaaat tgttgctcag ggagcgccga aggagattgt cactgctgaa
    15661 ctgattgagc gcatttatgg tctgcgatgc atgatcattg acgatccggt agccggaatg
    15721 ccgcttgtgg tgccgctcgg acgaacggca ccttcaaccg caaatagtta aactaagtgg
    15781 tctgccatca tggcatcctg ttttctggat gccatcgcat agcgcaataa cacgctacca
    15841 caagccacca tcccgccgat cagcgcggaa aggatcacaa taatcgcctt acccggaccg
    15901 tcttttttca ctggcaatga cgggcttaac tgatatttaa acggcgtaaa attcacatcg
    15961 ttgacatttg cttttgttaa ttgctcgaca agatactgcc gattacgtaa ttcaccgttc
    16021 agttccgcaa cgtcagtaac cgctttttca atttccagtt tgcgttcaat accgtctgcg
    16081 ccgagggaaa tagagaaatc ggggtcatct ttaacggcct gaccattact gtatacgggc
    16141 tttttaattc ctgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca
    16201 tcaagttgat ttttcgtttt aatgcgatcc tgagccagtt tttctttttc aaactgggtt
    16261 ttgatctcca gtttattacg gacgttttct atcgactctt tcaccaccaa cgcagagata
    16321 taatcgatat acccgctcaa aacggtctgc gcctctttac tggttggcgc ggtaaaactt
    16381 agcgtccagg atgtatacag tgacggttca tcttttttct tactagcatt gtcatcaacc
    16441 gctttcattt tttcgctcaa tgcaacaata gcgcgatgca aatccagttc atcgattttc
    16501 gcctctttta attagtccat cacataaggt gatgaacgca ggtactcttc cagcaagcta
    16561 acagactgaa acttcttgat aaacaggtta aatgcaccgc cacggtcgat attaacatcg
    16621 agatcaagaa cgcggagttt ggtaaaggtt ttctccaggt cctgccactg aatggcttcg
    16681 gcgggagtaa taacggctga gctggtccat ttttgcggca ggatgaaaga gatcagtaaa
    16741 cctgcgcagg caaatgcaaa aacgaccgcc atgaccgttt ttttggcccg ccataaaact
    16801 tcgattagat taagtaaatc aatttcatta ttactgggcg acgccagagg atactcagaa
    16861 aaatgagcgt cacttccctg tttaatattc aatgatgaca tggggtataa cctgaaaatg
    16921 agtccaatag gggaaaatct ccacggcgct cactttacca gttggtgaga attatccgaa
    16981 gctgaagttt gtaaattcct tccactgaat gcaacattca ctctatttaa gagaaatatt
    17041 catcaaacct ataaatcaaa caaataaaaa acccataatt attttctgta attatgggta
    17101 atgactccaa cttactgata gtgttttatg ttcagataat gccc
//
LOCUS       DAZPAB010000078        16786 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZPAB010000078 DAZPAB010000000
VERSION     DAZPAB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16786
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.078"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..190
                     /locus_tag="ACHUPG_002234"
     CDS             <1..190
                     /locus_tag="ACHUPG_002234"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861839.1"
                     /translation="PPHVKALPDTITQTSIHRTLQFIEFARSCRPDKAFTPHPAINRR
                     RLPNGRFFTYSPPSNVPA"
     gene            complement(156..1391)
                     /locus_tag="ACHUPG_002235"
     CDS             complement(156..1391)
                     /locus_tag="ACHUPG_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR0861840.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="ACHUPG_002236"
     tRNA            complement(1538..1614)
                     /locus_tag="ACHUPG_002236"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="ACHUPG_002237"
     tRNA            complement(1657..1743)
                     /locus_tag="ACHUPG_002237"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(2020..3051)
                     /gene="rsmC"
                     /locus_tag="ACHUPG_002238"
     CDS             complement(2020..3051)
                     /gene="rsmC"
                     /locus_tag="ACHUPG_002238"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR0861841.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            3154..3567
                     /gene="holD"
                     /locus_tag="ACHUPG_002239"
     CDS             3154..3567
                     /gene="holD"
                     /locus_tag="ACHUPG_002239"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR0861842.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            3536..3982
                     /gene="rimI"
                     /locus_tag="ACHUPG_002240"
     CDS             3536..3982
                     /gene="rimI"
                     /locus_tag="ACHUPG_002240"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR0861843.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            3997..4674
                     /gene="yjjG"
                     /locus_tag="ACHUPG_002241"
     CDS             3997..4674
                     /gene="yjjG"
                     /locus_tag="ACHUPG_002241"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR0861844.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            4765..6354
                     /gene="prfC"
                     /locus_tag="ACHUPG_002242"
     CDS             4765..6354
                     /gene="prfC"
                     /locus_tag="ACHUPG_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR0861845.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            6747..7352
                     /gene="osmY"
                     /locus_tag="ACHUPG_002243"
     CDS             6747..7352
                     /gene="osmY"
                     /locus_tag="ACHUPG_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR0861846.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            7469..7630
                     /locus_tag="ACHUPG_002244"
     CDS             7469..7630
                     /locus_tag="ACHUPG_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR0861847.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            7752..8825
                     /locus_tag="ACHUPG_002245"
     CDS             7752..8825
                     /locus_tag="ACHUPG_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR0861848.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            8822..9604
                     /locus_tag="ACHUPG_002246"
     CDS             8822..9604
                     /locus_tag="ACHUPG_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0861849.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(9818..10681)
                     /locus_tag="ACHUPG_002247"
     CDS             complement(9818..10681)
                     /locus_tag="ACHUPG_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR0861850.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(10653..12194)
                     /locus_tag="ACHUPG_002248"
     CDS             complement(10653..12194)
                     /locus_tag="ACHUPG_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR0861851.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            12452..13231
                     /gene="deoC"
                     /locus_tag="ACHUPG_002249"
     CDS             12452..13231
                     /gene="deoC"
                     /locus_tag="ACHUPG_002249"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR0861852.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            13358..14680
                     /gene="deoA"
                     /locus_tag="ACHUPG_002250"
     CDS             13358..14680
                     /gene="deoA"
                     /locus_tag="ACHUPG_002250"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR0861853.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            14732..15955
                     /gene="deoB"
                     /locus_tag="ACHUPG_002251"
     CDS             14732..15955
                     /gene="deoB"
                     /locus_tag="ACHUPG_002251"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR0861854.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            16012..16731
                     /gene="deoD"
                     /locus_tag="ACHUPG_002252"
     CDS             16012..16731
                     /gene="deoD"
                     /locus_tag="ACHUPG_002252"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR0861855.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         4001 a         4383 c         4511 g         3891 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcataccat caattcttaa aaagaattgc taccacggag gcgcattcta gtggttttca
     1801 gcttttcgtc aatagttaat tatcgcctga tgtgtaattg ctggaaaaat gtccatcagg
     1861 aaactagcgt gcaggtttgg taggcatgcg ggggcagatg ccagatgcga cgctggcgcg
     1921 tcttatctgg cctacgaagg gctaacgtgc aggttttgta ggtcggataa ggcgttcacg
     1981 ccgcatccga cacggtatgc ggcgacagat atcagatact caacctttct tcgcctggcg
     2041 ggtcataatg gcgcgatata ccttgaagcg cccggtttgc gcgataactt catggaagcc
     2101 aaatgtctca tccagcacgt ccgggtaagg caggaaggcg ttcgctacaa ttcgcagctc
     2161 gccgccgcta ttaagatgac gcaccgcgcc acgaatcagc gtttgcgccg catccaggct
     2221 ggtttgcatc ccatcgtgga acggcgggtt ggagatgatc atatcaaaac aacctttcac
     2281 ctcggaaaag acgttgctgg caaagacttc accttcaaca ccgttggctg caagtgttgc
     2341 gcggctggct tctaccgccg gggcagagac atcgcacaag gtgagacgaa ttttcggcga
     2401 atggcgcgca aaggcaactg aaagtacacc cgcgccacag ccgacatcca gcactttacc
     2461 tttcgtgtgc ggagttaacg tcgagagcag caactggcta ccgacatcca gaccgtcgcg
     2521 gctaaacacg ccaggcagcg ttttgaccgt caggccatcg acgctgtatt cgccccagaa
     2581 tttatccgca tcaaatactg gctgtttttc cagacgacca aaatagaggc cacagcgacg
     2641 agcgctgtcg actttattca acggcgcata atctgccagc atctgctcag cgctgcgcac
     2701 gccgctgcgg ttctcgccaa cgacaaaaat atctgtcccc actggcagca gagaaagtaa
     2761 attcatcaac tggaactggg cttccggttt gttcttcggc cagtagtaaa tcagcgtatc
     2821 gcaatctgcg acatcattcg ccgtggcgac cagactaaaa cgggcgttat cccccatctg
     2881 gcagcttaac acctgccaat ggtggaattg ctgggtatga gcacggctgg ccgcggtatc
     2941 taaacgcgcg ggcaggtcat cctgtaagtc tccggcaaac agaatacggc tttgttcgaa
     3001 atcatcactg tgacgcagca agacttcact tgccggggta aatgcagaca tggaatgctc
     3061 ctcaattgat actggcggcg attatagcca tatgttggcg cggtatcgac gaatttgcta
     3121 tatttgcgcc cctgacaaca ggagcgattc gctatgacat cccgacgaga ctggcagtta
     3181 cagcaactgg gcattaccca gtggtcgctg cgtcgccctg gcgcgttgca gggggagatt
     3241 gccattgcga gcccggcaca cgtccgtctg gtgatggtgg caaacgatct tcccgccctg
     3301 actgatcctt tagtgagcga tgttctgcgc gcattaaccg tcagccccga tcaagtgctg
     3361 caactgacgc cagaaaaaat cgcgatgctg ccgcaaggca gtcgctgcaa cagctggcgg
     3421 ttgggtactg acgaaccgct atcattggaa ggcgctcagg tggcatcacc ggcgctcacc
     3481 gaattacggg caaacccaac ggcacgcgcc gcgttatggc aacaaatttg cacatatgaa
     3541 cacgatttct tccctcgaaa cgactgattt accagcggct taccacattg aacaacgcgc
     3601 ccacgccttt ccgtggagtg aaaaaacctt tgccagcaac cagggcgagc gttatctcaa
     3661 ctttcagtta acgcaaaacg gcaaaatggc ggcgtttgcg attacgcaag tggtgctgga
     3721 tgaagctaca ttgttcaata ttgcggtcga tcccgactat cagcgtcagg ggctgggtag
     3781 ggcgctgctg gaacatctga tcgacgaact ggaaaaacgc ggcgtggcga cactatggct
     3841 ggaagtccgt gcctcaaatg ctgccgccat tgccctgtac gaaagtttag gctttaacga
     3901 ggcgacgatt cgccgcaatt actaccccac cacggacggt cgcgaagacg ccatcatcat
     3961 ggcgttgcca atcagtatgt aagacaaggt ggaataatga agtgggactg gattttcttt
     4021 gatgccgatg aaacgctgtt tacctttgac tcattcaccg gcctgcagcg gatgtttctt
     4081 gattacagcg tcacctttac cgctgaagat tttcaggact atcaggccgt taacaagcca
     4141 ctgtgggtgg attatcaaaa cggcgcgatc acttcattac agcttcagca cgggcgtttc
     4201 gagagctggg ccgaacggct gaaagttgaa gcaggcttgc ttaacgatgc ctttattaat
     4261 gcgatggcgg aaatctgtac gccgttgccg ggcgcggttt ctctgcttaa cgccattcgt
     4321 agcaacgcta aaatcggcat catcaccaac ggttttagcg ccttgcagca agtgcgtctg
     4381 gaacggacgg gcctgcgtga ttacttcgat ttgctggtga tttccgaaga agttggcgtt
     4441 gccaaaccga ataagaaaat tttcgattat gcgctggaac aggcgggcaa tcctgaccgt
     4501 tcacgcgtgc tgatggttgg cgacactgcc gagtccgata ttctcggtgg catcaacgcc
     4561 gggcttgcga cctgctggct gaatgcgcac aatcgcgagc aaccagaagg catcgcgccc
     4621 acctggaccg tttcttcgtt gcacgaactg gagcagctcc tgtgtaaaca ctgattgcct
     4681 cccccccgtt gatgggtaaa atagccgcaa tttttcgttt tcaacaagcg cggcgcgatg
     4741 ccgcttactc aagaagaaag aattatgacg ttgtctcctt atttgaaaga ggtggcgaag
     4801 cgccgcactt ttgccattat ttctcacccg gacgccggta aaactaccat caccgagaag
     4861 gtgctgctgt tcggacaggc cattcagacc gccggtacag taaaaggccg tggttccaac
     4921 cagcacgcta agtcggactg gatggagatg gaaaagcagc gtgggatctc cattactacg
     4981 tctgtgatgc agtttccgta tcacgattgc ctggttaacc tgctcgacac cccggggcac
     5041 gaagacttct cggaagatac ctatcgtacc ctgacggcgg tggactgctg cctgatggtt
     5101 atcgacgccg caaaaggtgt tgaagatcgt acccgtaagc tgatggaagt tacccgtctg
     5161 cgcgacacgc cgatcctcac ctttatgaac aaacttgacc gtgatatccg cgacccgatg
     5221 gaactgctcg atgaagttga gaacgagctg aaaattggct gcgcaccgat cacttggcca
     5281 attggctgcg gcaagctgtt taaaggcgtt taccaccttt ataaagatga aacctatctc
     5341 tatcagagcg gtaaagggca caccattcag gaagtccgca ttgttaaagg gctgaataac
     5401 ccggatctcg atgctgcggt tggtgaagat ctggcacagc agctgcgtga cgaactggaa
     5461 ctggttaaag gcgcgtctaa cgagttcgac aaagagctgt tccttgcggg cgaaatcact
     5521 ccggtattct tcggtactgc gctgggtaac ttcggcgttg atcatatgct gaatggcctg
     5581 gtggagtggg cgcctgcgcc gatgccgcgt cagaccgata cccgtaccgt agaagcgagc
     5641 gaagataaat ttaccggctt cgtatttaaa attcaggcca acatggatcc gaaacaccgc
     5701 gaccgcgtgg cgtttatgcg tgtggtgtcc ggtaaatatg aaaaaggcat gaaactgcgc
     5761 caggtgcgca ctgcgaaaga tgtggtgatc tccgacgcgc tgacctttat ggcgggtgac
     5821 cgttcgcacg ttgaagaagc gtatccgggc gatatcctcg gcctgcacaa ccacggcacc
     5881 attcagatcg gcgacacctt tacccagggt gagatgatga agttcaccgg tattccgaac
     5941 ttcgcaccag aactgttccg tcgtatccgc ctgaaagatc cgctgaagca aaaacagctg
     6001 ctcaaagggc tggtacagct ttccgaagag ggcgcggtgc aggtgttccg tccaatctcc
     6061 aacaacgatc tgatcgttgg tgcggttggt gtgctgcagt ttgatgtggt ggtagcgcgc
     6121 ctgaagagcg aatacaacgt tgaagcagtg tatgagtcag tcaacgttgc cactgcccgc
     6181 tgggtagaat gtgcagacgc gaagaaattc gaagagttca agcgtaagaa cgaaagccaa
     6241 ctggcgcttg atggcggcga taacctcgct tacatcgcta ccagcatggt caacctgcgc
     6301 ctggcacagg aacgttatcc ggacgttcag ttccaccaga cccgcgagca ttaattcttg
     6361 cctccagggc gcggtagccg ctgcgccctg tcaatttccc ttccttatta gccgcttacg
     6421 gaatgttctt aaaacattca cttttgctta tgttttcgct gatatcccga gcggtttcaa
     6481 aattgtgatc tatatttaac aaagtgatga catttctgat ggcgttaaat accgttcaat
     6541 gcgtagatat tagtatctaa agccgtcgat tgtcattcta ccgatattaa taactgattc
     6601 agaggctgta atggtcgtta ttcatcaccc atcgcttttg tgatggcgac cattgacttc
     6661 tgtagagggt gaagtctctc cctattcagc aatgcaacct cgtgttgcta ggctcaaatt
     6721 acgagcaaac atacaggaat aaatcgatga ctatgacaag actgaagatt tcgaaaactc
     6781 tgctggctgt aatgttgacc tctgccgtcg cgaccggctc tgcctacgcg gaaaacaacg
     6841 cgcagactac caatgaaagc gcagggcaaa aagtcgatag ctctatgaat aaagtcggta
     6901 atttcatgga tgacagcgcc atcaccgcga aagtgaaggc ggcgctggtg gatcatgaca
     6961 acatcaagag caccgatatc tctgtcaaaa ccgatcaaaa agtcgtgacc ctgagcggtt
     7021 tcgttgaaag tcaggcccag gccgaagagg cagtgaaagt ggcgaaaggc gttgaaggcg
     7081 tgacctctgt cagcgacaaa ctgcacgttc gcgacgctaa agaaggctcg gtgaagggct
     7141 acgcgggtga caccgccacc accagtgaaa tcaaagccaa actgctggcg gacgatatcg
     7201 tcccttcacg taaagtgaaa gttgaaacca ccgatggcgt ggttcagctc tccggtaccg
     7261 tagattctca ggcacaaagt gaccgtgctg aaagtatcgc caaagcggta gatggtgtga
     7321 aaagcgttaa aaatgatctg aaaactaagt aattcgtcct cccgaacttt gtcgggaggc
     7381 gcgatgtgca ccacactaaa aatatcgcat tgagtgattt tcacgctcat atcaagcggt
     7441 gacattaact atggtaaagg agacgcttat gtttcgttgg ggcatcatat ttctggttat
     7501 cgcgttaatc gccgccgcac ttgggtttgg tggtctggcc ggtaccgctg caggcgcagc
     7561 taaaattgtc tttgtcgtcg ggattattct gttcctagtg agtttgttca tgggccgaaa
     7621 acgaccctag atttcagacc attaagaaaa ttttatccaa agccagtcca gcggactggc
     7681 ttttgcggtt ttagcgaata ataaattgcg ttactttgtc tttctttcac aacaaaaaca
     7741 ggaaggcaga agtggggcag cgaatacctg taacgcttgg taatattgcg ccgttgtcgc
     7801 taaggccgtt ccagcctgga cgaatagctc tggtgtgcga aggcggcgga cagcgtggaa
     7861 ttttcacggc tggcgtgctg gatgagttta tgcgcgcgca gtttaatcct ttcgatcttt
     7921 atctcggcac atctgccggg gcgcagaacc tctcggcgtt tatctgcaat cagcccggtt
     7981 acgcgcgcaa agtcatcatg cgctatacca caaaacgcga atttttcgat ccattgcgct
     8041 ttgtccgtgg aggaaatctt atcgatctcg actggctggt ggaggccacc gcaagccaga
     8101 tgccgttgca aatggatacc gccgcgcggt tgtttgacag cggcaaatcg ttttatatgt
     8161 gcgcctgtcg tcaggatgac tacgcgccga attacttttt accaaccaaa caaaactggc
     8221 tggatgtgat tcgcgcctcc agtgcgatac ctggctttta tcgtagcgga gtgtcgctgg
     8281 aaggcattaa ctacctggat ggcgggatca gtgatgcgat tccggttaaa gaggcggcaa
     8341 ggcagggcgc taaaacgttg gtcgtcatcc gcactgtgcc gtcacaaatg tactacacgc
     8401 cgcagtggtt caaacgcatg gaacgctggc tgggtgacag tagcctgcag ccgctggtca
     8461 atctggtgca gcatcatgaa accagctatc gtgacattca gcaatttatt gagaaaccac
     8521 cgggcaagct gcggatatcc gaaatttatc cgccgaagcc attacatagt atcgcgcttg
     8581 gcagtcggat tccggcgctg cgtgaagact ataaacttgg gcgtttatgc ggtcgttatt
     8641 tcctcgccac ggttggcaag ctattaactg aaaaagcgcc gcttacccgc catctggtgc
     8701 cagtggtgac gccggaatcg attgtcattc cgcctgcgcc agtcgccaac gatacgctgg
     8761 ttgccgaagt gagcgacgct ccgcaggcga acgacccgac atttaacaat gaggatctgg
     8821 cttgatttgc cgttttatcg acactcactg ccattttgat ttcccgcctt ttattggcga
     8881 tgaagaggcc agcctgcaac gcgcggcaca agcgggcgta ggcaagatca ttgttccggc
     8941 gacagaggcg gaaaattttg cccgtgtgca ggcgttagcg gaaaattatc aaccgctgta
     9001 tgccgtattg ggcttgcatc ctggtatgtt ggaaaaacat agcgatgtgt ctcttgagca
     9061 gctacagcag gcgctggaaa ggcgtccggc gaaggtggtg gcggtggggg agatcggtct
     9121 ggatctcttc ggcgacgatc cgcaatttga gaggcagcag tggttactcg acgaacaact
     9181 gaaactggcg aaacgctacg atctgccggt gatcctgcat tcacggcgca cgcacgacaa
     9241 actggcgatg catcttaaac gccacgattt gccgcgtgct ggtgttgtcc acggattttc
     9301 tggcagccag caacaggctg aacggtttgt acagctgggc tacaaaatcg gcgtaggcgg
     9361 tactatcacc tatccacgcg ccagtaaaac ccgcgatgtc atcgcaaaat taccgctggc
     9421 atcgttgttg ctggaaaccg atgcgccgga tatgccgctc aacggttttc aggggcagcc
     9481 taaccgcccg gaacaggctg cccgtgtgtt tgccgtgctt tgcgagttac gcccggaacc
     9541 cgcggatgag attgccgaag tgttgcttaa taacacgtat gcggtgttta acgttcgtgg
     9601 gtagtttgcc cttcgtaggc agcatccggc agtcggcgca taatgcctga tgcgacgctt
     9661 aacgcgtctt atcatgccta caggtttgtg ccgaaccgta ggccggataa ggcgttcacg
     9721 ccgcatccgg cagttggcgc acaatgcctg atgcgacgct tgacgcgtct tatcaggcct
     9781 acaagtctgt gccgaaccgt aggccgtatc cggcatgtca caaatagagc gccggaaata
     9841 tcaaccggct caccccgcgc acctttaatg catcagccaa cggctcaacg tcttccggcg
     9901 tggcgctcgc ccagctttgc gcctcgccat acacgccgtg ggcatgaaac gcgttcaggc
     9961 gaaccggaac atcaccaagt cccttgataa acgccgccag ttcttcgatg tgttgcaaat
    10021 aatccacctg gcctgggatc atcagcaaac gcagttccgc cagcttgccg cgctctgcca
    10081 gcaaatagat gctgcgctta atctgttgat tatcgcgtcc ggttaattgt tgatgacatt
    10141 cgctacccca tgctttgaga tcgagcattg cgccgtcaca caccgggagt aatttttccc
    10201 agccggtttc gctcaacata ccgttactgt ccaccagaca ggtgagatgg cgcagttgcg
    10261 gatcgttttt gatagcagta aacagcgcca ctacaaacgg cagttgggtc gtggcttcgc
    10321 cgccgcttac cgttatccct tcaataaaca gcaccgcttt gcgcacatgg ctaagcactt
    10381 cgtccacgct catggattgc gccattggtg tggcatgttg cggacacatc ttcaggcagg
    10441 tatcacattg ctcgcagacc acagcgttcc acaccacttt gccgtcaaca atctgcaacg
    10501 cctgatgcgg acactgtggc acgcactccc cacagtcatt gcaacgtccc atcgtccacg
    10561 gattgtgaca gtttttgcag cgcagattgc agccctgcaa aaacagagcc agacgactgc
    10621 ctggcccgtc aacgcaggag aaggggataa tcttactgac taaagcgcat ctgctgttca
    10681 tggcttatca cgcgcggctg gcgttccaga atacgagtgt tgcgtgcggc ttcttcgccc
    10741 agccaggtgg tgttggtgcg tgaaccttcg gcgcgatatt tttctaaatc cgacaaacgc
    10801 accatataac cggtaacgcg aaccagatcg ttaccgctga cattggcggt aaattcacgc
    10861 attccggctt taaaggcacc gaggcaaagc tgtaccagtg cctgcgggtt acgtttgatg
    10921 gtttcgtcga gcgtcagaat gtcgctgatg ccggaataat aataagcatg atgcggggcg
    10981 acagtttgca gatgggtgat cggatctggc tcatcgccat acggcaaacg cgcgcccggc
    11041 gtggtgccga tatcggaact gatccccgac tgtgcgtgta acatggcgcg tttttgccag
    11101 ccatatttca cgggggtatt ggcgacaaac tccgccagtt gcgcgctgat gcgataaccc
    11161 acttcatttg cggcggcttc tttaccgtag cgcgcggcaa tcccttcttt ctcacacagc
    11221 aagttaaccg cttccgccag cccatacatg ccaaacattg gcacaaaacg ttcagggtta
    11281 atcagccctt ctttcaccag gaagctattc tcaaagaagt gtgattgttg atagaggaat
    11341 tcacaccgcg catcgatgat ggcgatctgc tgctggcagt agtgcggtag agtgcgcgta
    11401 aagaagtcat ccagcgattc gctgcgctcg gcaatggctt tcaggttaag gcgtaccagc
    11461 gtgctgccac cacccgccag cggcagtgag ttgtaacagc tcacaatccc gtagccccct
    11521 tttgtgaaaa ttttatcatg caccggaccg ttggcgatgt gcggtttgct acattcacag
    11581 atgttcttcg ccacttccag cagcaggtca tcaggggtga tttcaggatc gtagataaag
    11641 gtcaggttcg gtgaaacctg cgcgtctgca cgtaagatcg cgcgggtaat gggcgaatca
    11701 gacgggccga tattggcgtg cataaaggca tctggcaggg ttctgtcgag gtaacgccag
    11761 aaacgtttta ttcgaacatc gatctcgttt tgtgttagaa ttctaacata cggttgcaac
    11821 aacgcatcca gttgccccag gtagaccggc atcgatgtga ccgacggtac gtggtggtaa
    11881 agaatggtca gcagagagag tgcgtcatca agatctttcg cgccttccag ctccagccat
    11941 tcggaaccgt tcgccagaaa acgagcgtaa tcgggtaaga catagcgcgg tttgtacggc
    12001 gcatgacctt caaacatatc gcagattaca ccttcatcca gcgcgcggcg ggcttcggca
    12061 ggaagctgtg ggtaaggcag attgttttct gcttccagtg ccagaaaatg gcgcttctgc
    12121 tccgggctaa gcactgggct ggtgacaatt tgctggcaac gttgttgcag tgcattttca
    12181 tgagaagtgg gcatcttctt ttccttttat gccgaaggtg atgcgccatt gtaagaagtt
    12241 tcgtgatgtt cactttgatc ctgatgcgtt tgccaccact gacgcattca tttgaaagtg
    12301 aattatttga accagatcgc attacagtga tgcaaacttg taagtagatt tccttaattg
    12361 tgatgtgtat cgaagtgtgt tgcggagtag atgttagaat actaacaaac tcgcaaggtg
    12421 aattttattg gcgacaagcc aggagaatga aatgactgat ctgaaagcaa gcagcctgcg
    12481 tgcactgaaa ttgatggacc tgaccaccct gaatgacgac gacaccgacg agaaagtaat
    12541 tgctctgtgt catcaggcca aaactccggt cggcaatacc gccgctatct gtatctatcc
    12601 tcgttttatc ccgattgctc gcaaaactct gaaagagcag ggcaccccgg aaatccgtat
    12661 cgctacggta accaacttcc cacacggtaa cgacgacatc gaaatcgcgc tggcagaaac
    12721 ccgtgcggca atcgcctacg gtgctgatga agttgacgtg gtgttcccgt accgcgcgct
    12781 gatggcgggt gacgagcagg ttggttttga cctggtgaaa gcctgtaaag aggcttgcgc
    12841 ggcagcgaat gtactgctga aagtgatcat cgaaaccggc gaactgaaag acgaagcgct
    12901 gatccgtaaa gcgtctgaaa tctccatcaa agcgggtgcg gacttcatca aaacctctac
    12961 cggtaaagtg gctgtgaacg cgacgccgga aagcgctcgc atcatgatgg aagtgatccg
    13021 tgatatgggc gtagaaaaaa ccgttggttt caaaccggcg ggcggcgtgc gtactgcgga
    13081 agatgcgcag aaatatctcg ccatcgcaga tgaactgttc ggtgctgact gggctgatgc
    13141 gcgtcactac cgctttggtg cttccagcct gctggcaagc ctgctgaaag cgctgggcca
    13201 cggcgacggt aagagcgcca gcagctacta agtaagatgc tttacgcctg atgcgctgcg
    13261 cttatcaggc ctacgagacg tatctacccg taggccggat aaggcgtaga cgcatccggc
    13321 aaaagccgcc tcatactctt ttcctcggga ggttaccttg tttctcgcac aagaaattat
    13381 tcgtaaaaaa cgtgatggtc atgcgctgag cgatgaagaa attcgtttct ttatcaacgg
    13441 tattcgcgac aacactattt ccgaagggca gattgccgcc ctcgcgatga ccattttctt
    13501 ccacgatatg acaatgcctg agcgtgtctc gctgaccatg gcgatgcgag attcaggaac
    13561 cgttctcgac tggaaaagcc tgcatctgaa tggcccgatt gttgataaac actcgaccgg
    13621 cggcgtcggc gatgtcactt cgctgatgtt ggggccgatg gtcgcagcct gcggcggcta
    13681 tattccgatg atctccggtc gcggcctcgg tcatactggc ggtacgctcg acaaactgga
    13741 atccatccct ggcttcgaca ttttcccgga tgacaaccgt ttccgcgaaa ttattaaaga
    13801 cgtcggcgtg gcgattatcg gtcagaccag ttcactggct ccggctgata aacgtttcta
    13861 cgcgacccgt gatattaccg caaccgtgga ctccatcccg ctgatcactg cctcgatcct
    13921 ggcgaagaaa ctggcggaag gtctggacgc gctggtgatg gacgtgaaag tgggtagcgg
    13981 cgcgtttatg ccgacctacg aactctctga agcccttgcc gaagcgattg ttggcgtggc
    14041 taacggcgct ggcgtgcgca ccaccgcgct gctcaccgac atgaatcagg tactggcctc
    14101 cagtgcaggt aacgcggttg aagttcgtga agcggtgcag ttcctgacgg gtgagtatcg
    14161 taacccgcgt ctgtttgatg tcacgatggc gctgtgcgtg gagatgttga tctccggcaa
    14221 actggcgaaa gatgacgccg aagcgcgcgc gaaattgcag gcggtgctgg acaacggtaa
    14281 agcggcagaa gtctttggtc gtatggtagc ggcacaaaaa ggcccgaccg acttcgttga
    14341 gaactacgcg aagtatctgc cgacagcgat gctgacgaaa gcagtctatg ctgataccga
    14401 aggttttgtc agtgaaatgg atacccgcgc gctggggatg gcagtagttg caatgggcgg
    14461 cggtcgtcgt caggcatctg acaccatcga ttacagcgtc ggctttactg atatggcgcg
    14521 tctgggcgac caggtagacg gtcagcgtcc gctggcggtt atcctcgcga aagacgaaaa
    14581 cagctggcag gaagcggcga aagcggtgaa agcggcaatt aaacttgccg ataacgcacc
    14641 ggaaagcaca ccaactgtct atcgccgtat cagcgaataa cggtatactg atctgatcat
    14701 ttaaatttga agcactgagt acggagaaca tatgaaacgt gcatttatta tggtgctgga
    14761 ctcattcggc atcggcgcta cagaagatgc agaacgcttt ggtgacgtcg gggctgacac
    14821 cctgggtcat atcgcagaag cttgtgccaa aggcgaagct gataatggtc gtaaagggcc
    14881 gctcaatctg ccaaatctga cccgtctggg gctggcgaaa gcacacgaag gttctaccgg
    14941 tttcattccg gcgggaatgg acggcaacgc tgaagttatc ggcgcgtacg catgggcgca
    15001 cgaaatgtca tccggtaaag ataccccgtc tggtcactgg gaaatcgccg gtgtcccggt
    15061 tctgtttgag tggggatatt tctccgatca cgaaaacagc ttcccgcaag agctgctgga
    15121 taaactggtc gaacgcgcta atctgccggg ttacctcggt aactgccact cttccggtac
    15181 ggtcattctg gatcaactgg gcgaagagca catgaaaacc ggcaagccga ttttctatac
    15241 ctccgctgac tccgtgttcc agattgcctg ccatgaagaa actttcggtc tggataaact
    15301 ctacgaactg tgcgaaatcg cccgtgaaga gctgaccaac ggcggctaca atatcggtcg
    15361 tgttatcgct cgtccgttta tcggtgacaa agctggtaac ttccagcgta ccggtaaccg
    15421 tcacgacttg gctgttgagc cgccagcacc gaccgtgctg cagaaactgg ttgatgaaaa
    15481 acacggccag gtggtttctg tcggtaaaat tgcagacatc tacgccaact gcggtatcac
    15541 caaaaaagtg aaagcgactg gcttggacgc gctgtttgac gccaccatca aagagatgaa
    15601 agaagcgggt gataacacca tcgtcttcac caacttcgtt gacttcgact cttcctgggg
    15661 ccaccgccgc gacgtcgccg gttatgctgc gggtctggaa ctgttcgacc gccgtctgcc
    15721 ggagctgatg tctctgctgc gcgatgacga catcctgatc ctcaccgctg accacggttg
    15781 cgatccgacc tggaccggta ctgaccacac gcgtgaacac attccggtac tggtatatgg
    15841 cccgaaagta aaaccgggct cactgggtca ccgtgaaacc ttcgcggata tcggccagac
    15901 tctggcaaaa tattttggta cttctgatat ggaatatggc aaagccatgt tctgatggat
    15961 ttgggcggag cgttgactcc gcctttgtta tgtcacaaaa aggataaaac aatggctacc
    16021 ccacacatta atgcagaaat gggcgatttc gctgacgtag ttttgatgcc aggcgacccg
    16081 ctgcgtgcga agtatattgc tgaaactttc cttgaagatg cccgtgaagt gaacaacgtt
    16141 cgcggtatgc tgggcttcac tggtacttac aaaggccgca aaatttccgt aatgggtcac
    16201 ggtatgggta tcccgtcctg ctccatctac accaaagaac tgatcaccga tttcggcgtg
    16261 aagaaaatta tccgcgttgg ttcctgtggc gcagttctgc cgcacgtaaa actgcgcgac
    16321 gtcgttatcg gtatgggggc ctgcaccgat tccaaagtta accgcatccg ttttaaagac
    16381 catgactttg ccgctatcgc tgactttgac atggtgcgta acgcagtaga tgcagctaaa
    16441 gcactgggcg ttgatgctcg cgtgggtaac ctgttctccg ctgacctgtt ctactctccg
    16501 gacggcgaaa tgttcgacgt gatggaaaaa tacggcatcc tcggcgtgga aatggaagcg
    16561 gctggtatct acggcgtggc tgcagagttt ggcgcgaaag ccctgaccat ctgcaccgtg
    16621 tctgaccaca tccgcactca cgagcagacc actgccgctg agcgtcagac caccttcaac
    16681 gacatgatca aaatcgcact ggaatccgtt ctgctgggcg ataaagagta attgtgtttc
    16741 gctgaaaggc gattgtcgtg ttaagccgga gtgggggact gctccg
//
LOCUS       DAZPAB010000079        16605 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZPAB010000079 DAZPAB010000000
VERSION     DAZPAB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.079"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            221..373
                     /gene="mokC"
                     /locus_tag="ACHUPG_002253"
     CDS             221..373
                     /gene="mokC"
                     /locus_tag="ACHUPG_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR0861856.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(477..1607)
                     /gene="dnaJ"
                     /locus_tag="ACHUPG_002254"
     CDS             complement(477..1607)
                     /gene="dnaJ"
                     /locus_tag="ACHUPG_002254"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR0861857.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1696..3612)
                     /gene="dnaK"
                     /locus_tag="ACHUPG_002255"
     CDS             complement(1696..3612)
                     /gene="dnaK"
                     /locus_tag="ACHUPG_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR0861858.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3989..4393
                     /locus_tag="ACHUPG_002256"
     CDS             3989..4393
                     /locus_tag="ACHUPG_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR0861859.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4419..5132
                     /gene="msyB"
                     /locus_tag="ACHUPG_002257"
     CDS             4419..5132
                     /gene="msyB"
                     /locus_tag="ACHUPG_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR0861860.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5281..5847
                     /gene="satP"
                     /locus_tag="ACHUPG_002258"
     CDS             5281..5847
                     /gene="satP"
                     /locus_tag="ACHUPG_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR0861861.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5882..6469)
                     /gene="mog"
                     /locus_tag="ACHUPG_002259"
     CDS             complement(5882..6469)
                     /gene="mog"
                     /locus_tag="ACHUPG_002259"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR0861862.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6584..7537)
                     /gene="tal"
                     /locus_tag="ACHUPG_002260"
     CDS             complement(6584..7537)
                     /gene="tal"
                     /locus_tag="ACHUPG_002260"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR0861863.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7816..9246
                     /locus_tag="ACHUPG_002261"
                     /pseudo
     CDS             7816..9246
                     /locus_tag="ACHUPG_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            9316..10092
                     /gene="yaaA"
                     /locus_tag="ACHUPG_002262"
     CDS             9316..10092
                     /gene="yaaA"
                     /locus_tag="ACHUPG_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR0861864.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(10245..10541)
                     /locus_tag="ACHUPG_002263"
     CDS             complement(10245..10541)
                     /locus_tag="ACHUPG_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR0861865.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10755..12041)
                     /gene="thrC"
                     /locus_tag="ACHUPG_002264"
     CDS             complement(10755..12041)
                     /gene="thrC"
                     /locus_tag="ACHUPG_002264"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR0861866.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(12042..12974)
                     /gene="thrB"
                     /locus_tag="ACHUPG_002265"
     CDS             complement(12042..12974)
                     /gene="thrB"
                     /locus_tag="ACHUPG_002265"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR0861867.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12976..15438)
                     /gene="thrA"
                     /locus_tag="ACHUPG_002266"
     CDS             complement(12976..15438)
                     /gene="thrA"
                     /locus_tag="ACHUPG_002266"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR0861868.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15464..15583)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15519..15584)
                     /gene="thrL"
                     /locus_tag="ACHUPG_002267"
     CDS             complement(15519..15584)
                     /gene="thrL"
                     /locus_tag="ACHUPG_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR0861869.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15798..16484)
                     /locus_tag="ACHUPG_002268"
     CDS             complement(15798..16484)
                     /locus_tag="ACHUPG_002268"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR0861870.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         3849 a         4421 c         4120 g         4215 t
ORIGIN      
        1 aaatgcgagg cgtcttcagg attagaatcg ataaaacaaa atgggttaaa gagagcgata
       61 taatagcggc gggtgcttga ggctgtctgt ctcaggcatt agctgaacgg cagatagaga
      121 aaagccccga gtgatatttt accatcaacc cgaggcctcc tatatgctga acacatgtag
      181 agtgcctctt actgaccgta aggtcaagga gaagagagca atgaagcagc ataaggcgat
      241 gattgtcgcc ctgatcgtca tctgtatcac cgccgtagtg gcggcgctgg taacgagaaa
      301 agacctctgt gaggttcaca tccgaactgg ccagacggag gttgctgttt tcacggctta
      361 cgaatccgag taagagcaac ggcggggagt gatccccgcc actttacagg tgctcgcata
      421 tcttcaacgc accctatttt tacccaggcc tgcccacggg caggcttttg gggaggttag
      481 cgggtcaggt cgtcaaaaaa cttcttcaca ccatcaaaga agctctttga gcgcgggctg
      541 ttgtgctcgc cggttgggcc accgaagctt tcttgcagct cttgcagcag ctgtttctgc
      601 ttttcgttca ggcctaccgg tgtttcgacg acaacgcggc acagcaaatc accctgtgcg
      661 ccaccgcgga cagacttgac gcctttaccg cgcatacgga atagcttacc ggtctgggtt
      721 tcgccaggca ctttcagttt gacgcgacca tcaagggtcg gtacttcgat ttcgccaccc
      781 agcgccgcca tagcgaagtt gatcgggact tcgcaataca ggttgttgcc ttcacgctcg
      841 aaaatcgggt gctgtttaac ctgaacctga acgtacagat cgcctgccgg tgcgccatgc
      901 tcgcccgctt caccttcgcc cgcaagacgg atgcggtctc cagtgtccac ccctgccggg
      961 attttaacgg acagcgtttt gctgcgctca acacgaccat gaccatgaca tttgttgcac
     1021 ggatctttga tcagcgtacc gcggccctga cagtgtggac aggtctgctg tacagcgaag
     1081 aatccctggc gcatctgcac ctgaccagaa ccatgacagg tcggacaggt ctgcggctgt
     1141 gtacctggtt ttgcaccgct accgtggcaa acgtcacact cttccagagt cggaatgcgg
     1201 atctctttgg tcacgccacg tacagcttct tcgagggtga gctccatgtt atagcgtaaa
     1261 tcagcaccgc gcgccgcacg ttgacgacca cgtccgccgc caaaaatatc gccgaaaacg
     1321 tcaccaaaaa tatcgctgaa gtctgcgccg ccgccaaaac caccgccgcc catgcctcct
     1381 tgctcaaacg cagcatgacc atactgatcg tatgccgcac gtttttgcga gtcggtcaga
     1441 acttcataag cttccttgat ctctttaaat ttcgcctcgg cctctttgtc accctggtta
     1501 cggtccgggt ggtatttcat ggccaggcgt ttgtaggcct ttctgatttc acgctcttcc
     1561 gctgttttgg aaacgcctaa aatctcgtaa taatcttgct tagccatctt ttttaaattg
     1621 cccctagatg aatgcacggg cggagaggaa attccccttc gcccgtgtca gtataattac
     1681 ccgtttatag ggcgattatt ttttgtcttt gacttcttca aattcagcgt cgacaacatc
     1741 gtcatctttc gcgttgtttg cagaagcatc agcaccggca gtctgctgct gggcatgttg
     1801 ctgctgggcg atttccatca gtttctggga aacctgtgcc agttcctgca ttttcgcttc
     1861 gatagcggct ttgtcttcac ctttcagagc agtttccagt gcagtcagcg cagactcgat
     1921 agcagttttg tcgtcagccg gcagtttgtc gcctgcttct tcaacctgct tacgggtgct
     1981 gtgcagcaga tggtcgccct ggttgcgagt ctgtaccagc tcttcaaact tacggtcagc
     2041 ttcggcgtta gcttctgcgt cgcgtaccat tttctggatt tcatcttcgt tcagaccaga
     2101 agaagccttg atggtgatct tctgctcttt accgctgttt ttatctttcg cggaaacgtg
     2161 caggataccg tcagcatcga tatcgaaggt aacttcgatc tgcggcatgc cgcgcggtgc
     2221 cgggttgata ccatctaggt tgaactgacc cagagatttg ttatcagccg cacgtttacg
     2281 ttcaccctgc agcacatgga tggttaccgc agactggttg tcttcagcgg tagagaacac
     2341 ctggctgtgc ttggtcggga tagtggtgtt tttcgcgatc agcgtcgtca tcacaccgcc
     2401 catggtttcg atacccagag acagcggggt aacgtccagc agcagtacgt cttttacgtc
     2461 accagtcaga acaccaccct gaacagcagc accgattgct acagcttcgt ccgggttaac
     2521 gtctttacgc ggctctttac caaagaactc agcaactttc ttctgaacca ttggcatacg
     2581 agtctgacca ccaacgagga taacgtcgtc gatatcagat acggacaggc cagcatcctg
     2641 cagtgcaact ttcagcggct caatggaacg gtttaccaga tcttcaacca ggctttccag
     2701 tttcgcacga gtcactttga tgttcatgtg tttcggaccg gtcgcgtctg cagtgatgta
     2761 tggcaggtta acgtcggtct gctgagcgga agacagttcg attttcgctt tttctgccgc
     2821 ttctttcagg cgctgcattg ccagcggatc gttgcgcagg tcaatgccct gatctttctt
     2881 gaattcttca accagatagt tgatcagacg gctgtcgaag tcttcaccac ccaggtgggt
     2941 atcaccgttg gttgccagaa cttcgaaggt tttttcgccg tcaacttcgt cgatttcgat
     3001 aatagaaata tcgaaagtac caccccccag gtcataaacc gcgatagtac ggttgccagt
     3061 gcctttgtcc agaccgtaag ccagcgcagc tgcggtcggt tcgttgatga tacgttttac
     3121 ttccagacca gcgatacggc ctgcgtcttt ggttgcctga cgctgagcat cgttaaagta
     3181 tgccggtacg gtgataacag cttcagttac cggttcaccc aggtaatctt cagcggtttt
     3241 cttcattttt ttcagcactt cagcagaaat ctgcggcggt gccattttct ggcctttaac
     3301 ttcgacccat gcgtcgccgt tatcagcagc aataattttg aacggcatga tggaaacatc
     3361 acgctgtact tcttcgtcct ggaagcggcg gccaatcagg cgtttaatcg caaacagggt
     3421 gttttgcggg ttcgtcactg cctgacgttt agccggctga ccaaccagag tttcaccatc
     3481 ctgggtatag gcaatgatag aaggcgtggt gcgatcgcct tcggcgttct ccagcacgcg
     3541 aggaatggtg ccatccataa tcgctacaca agagttggta gtacccaggt cgataccaat
     3601 tattttaccc atctaaacgt ctccactata tattcggtca tcatgtggtt gtgagtctgt
     3661 aataggggcg aaacgtctgg tttcaactgc ccaatttcat ttttttgcag actcactcac
     3721 tgcggttgac tactaaatgg ggtcgtaacc cacgtcatca agggggagat gcaaaaattt
     3781 tttgtgcttt tgcgcagaaa tttcgcgata tggcaggaca agacgtaaaa ttgacagcaa
     3841 ttttattcat ataaagaatg aatcgcagcc aaggcgttca tcaaaaaatt gtaataaaaa
     3901 gaaaagatta cgtgcctgaa tcttctcttt atcagcagta aactagtggg tattcatccc
     3961 cctacctctt cccactaaga gaatccttat gaaatccgtt tttacgattt ccgccagcct
     4021 ggcgattagc ctgatgctgt gctgcacggc gcaggcaaac gaccataaac tcctcggcgt
     4081 cattgcaatg ccgcgtaacg aaaccaacga tctggcgctg aaacttcctg tttgtcgcat
     4141 tgtgaaacgc atacaactct ctgccgacca tggcgattta cagttaagtg gtgcatcggt
     4201 ttatttcaaa gccgcccgta gcgccagtca gagcctgaat attccttcag aaataaaaga
     4261 agggcaaacc actgactgga tcaacattaa cagcgataac gacaataaac gctgcgtctc
     4321 aaaaatcacc ttttccggtc atacggtgaa ctcatcggat atggccacgc tgaaaattat
     4381 cggcgacgac taacgccaga atatagaagc cacaaaaaat gaatgttaat tacctgaatg
     4441 attcagatct ggattttctt cagcattgta gtgaggaaca gttggcaaat ttcgcccgat
     4501 tgctcaccca taatgaaaaa ggcaaaactc gcctctccag catactgatg cgcaacgaac
     4561 tgtttaaatc gatggaaggg catcccgagc aacatcgccg caactggcag ctgattgccg
     4621 gagaattaca gcattttggt ggcgatagta tcgccaacaa actgcgcgga cacggtaaat
     4681 tgtatcgggc cattttgctc gatgtttcaa agcgattgaa gctgaaagcc gacaaagaga
     4741 tgtctacgtt tgaaattgag caacagttac tggaacaatt tctgcgtaat acctggaaga
     4801 aaatggacga ggaacataag caggagtttc tgcacgcggt cgatgccagg gtgaatgagc
     4861 tggaagagct gctgccgctg ctgatgaaag acaaattatt ggcaaaaggt gtgtcgcatt
     4921 tgctttccag ccaactgacc cgcattttac gcacccacgc agcaatgagc gtacttgggc
     4981 atggtttgct gcgcggcgcg gggctgggag gccctgtggg cgcagcatta aatggggtta
     5041 aagcggtcag cggcagcacc tatcgcgtga cgattccagc cgtactgcaa atcgcctgcc
     5101 tgcgccggat ggttagcgcc actcaggtct gataccggaa accggggaaa attttcattt
     5161 tcccctcaaa agatcgtaga cactgcccca ctggctgatt attatgccgc gccctgaaaa
     5221 cactacagtt attcagggaa attatttcac cattcattcg atgatgattt ttgaggaatt
     5281 atgggcaaca ctaagttggc taatccggca ccgctgggcc tgatgggctt cggcatgacc
     5341 accattctgc ttaacctgca caacgtgggt tatttcgctc tggacggtat tattcttgcc
     5401 atgggcattt tctacggcgg catcgcgcaa atttttgctg gtctgctgga gtacaaaaaa
     5461 ggcaacactt tcggtttaac cgcattcacc tcttacggtt ctttctggct gacgctggtt
     5521 gcgattctgc tgatgccgaa actgggtctg accgatgcgc caaatgcaca gttccttggt
     5581 gtctacctgg gtctgtgggg cgtatttacg ctgtttatgt tcttcggcac tctgaaaggc
     5641 gcgcgcgtcc tgcaattcgt tttctttagc ctgaccgtgc tgtttgccct gctggcgatc
     5701 ggtaacattg ccggtaatgc cgcgatcatc cactttgccg gctggattgg tctgatctgc
     5761 ggtgccagcg caatctatct ggcgatgggt gaagtgctga acgagcagtt tggtcgcacc
     5821 gttctgccga ttggtgaatc ccactaattg tcttgagatc cccccgctcg gggggatttt
     5881 tttattcgct aacgtcgcgt cttgcactct tcggtctgaa tgcagcaacc acttctggtg
     5941 ccgtttcaac gtatggccct tccagcaact gaatgcagta cggtacgctg gcaaaaatac
     6001 catgcaccac aacgttaccc tcagcgtcct tcacaccttc cagcgtctct ttaatagact
     6061 tcggctgacc gggtaagtta aggatcagcg cctgtttgcg aatcacgccc acctgacgcg
     6121 aaaggatcgc agttggtaca aaatgcaggc tgatctggcg catctgttca ccaaagccag
     6181 gcatctcgcg gtccgctact gccagcgtcg catcgggcgt tacgtcacga cgcgccgggc
     6241 cagttccgcc cgtggtgagc accagatggc aactcatttc atccaccagc tcacacaacg
     6301 tttgctcgat gatcgcctgc tcatcgggga ttaagcgggt ttccagttca aacggcgtgg
     6361 ttagcgccga tgtcagccat tcttccagcg ctggaatgcc tttatcctga taaacgccgc
     6421 tggatgcgcg atcagagatg gaaactaagc caatacgtaa agtattcatc aagatttttc
     6481 cgtttaaaca atctcgttaa acagaatgat acactgcgaa gggagtgaca gacaggcttc
     6541 agaagaggta gcgtgaccga cttcccggtc acgctaagaa tgattacagc agatcgccga
     6601 tcattttttc cagtttttcc tggtcaatag caaacttacg gataccttcc gccagtttat
     6661 ctactgccat tggatcctgg ttgtgctgcc acaggaactc ggactcagtg atacgcgccg
     6721 gacgcgcttt cacttcgccg gtgtaagaca gtttacgttc gatagcccct tcgctctccg
     6781 ccagctcttt cagcagtgcc ggtgcgatgg tcagacggtc acagcctgcc agttccagaa
     6841 tttcgccgat gttacggaag cttgcgccca taaccacggt ttcataaccg tgctctttgt
     6901 agtactggta gatttcagat acagaaacca cgcccggatc ttctgccgga gcgtactctt
     6961 tcttatcggt attcgctttg taccagtcaa gaatacggcc aacaaacggc gagatcagga
     7021 acacgcccgc ctccgcacaa gcacgagcct gagcgaagga gaacagcagg gtcaggttac
     7081 agttgatacc ttctttttcc agctgttctg cagcacggat accctgccag gtagaagcca
     7141 gtttgatcag aatacgatcg ttgctaatac ctgcatcgtt gtagagtttg atcaggcgtt
     7201 ttgctttcgc aattgacgct tcagtgtcat aggaaagacg cgcatcaact tcagttgaga
     7261 tacggcccgg aaccagtttc aggatttcca gaccaatatt tactgccagt ttgtcggtcg
     7321 cgtccacgat ctgctgcgcg cgatcgttgc tctgctgttt cgcccaggcg acagcatcat
     7381 caatcaactt acggtattcc ggaatctgcg ctgcgttaag aatgagagaa gggttggttg
     7441 tggcatcctg cggttgatac agcttcattg ccgcgatgtc cccagtgtcg gccactacgg
     7501 tggtgtactg acgaagggag gtcaatttgt ccgtcatgat agtatttctc tttaaacagc
     7561 ttgttagggg gatgtaaccg gtctgccctg atgatatcac gacgcaacgt cagcgcaacc
     7621 gccgacaaga cggttatcgc caggcgagac tgtttcggat ttctgacact gcgttgatat
     7681 cgcccaccat ttttatacaa aacctcatgt atgctacgca gaagttatca agtacctcgt
     7741 agcgtatata cttcttaaac aattggtaac gtttacacag gaaagtcatc gcgaccggca
     7801 ataagaggga tatgcatgcc agattttttc tcctttatta acagcgtcct ttggggatcg
     7861 gtaatgattt acctgctctt cggcgcaggt tgttggttca cttttcgcac cggatttgtg
     7921 cagtttcgct acatccgcca gtttggcaaa agtcttaaaa atagcattca tccacagcca
     7981 ggcggtttaa cctcatttca gtcattgtgt accagtcttg cggcgcgcgt gggtagcggc
     8041 aacctggccg gcgttgcgct ggcaattacc gccggtggac caggtgccgt cttttggatg
     8101 tgggttgctg cgtttatcgg catggcgacc tcgtttgccg aatgttccct tgcacaactt
     8161 tataaagaac gtgacgtcaa tgggcagttt cgtggcggac cggcatggta tatggcgcgc
     8221 gggctgggga tgcgctggat gggcgttctg ttcgccgtct ttttgctcat cgcctacggc
     8281 ataattttca gcggagttca ggcgaacgcc gttgcccgag ccctgagttt ttcttttgat
     8341 tttcctccgc tggtgacggg cattattctc gctgtctttg ctctgctggc aatcactcgt
     8401 ggtcttcatg gcgtcgcccg gctcatgcag gggtttgtcc cgttgatggc gataatctgg
     8461 gtactgacca gcctggtgat ttgcgtaata aatatcgggc aacttcccca cgtcatttgg
     8521 tctatttttg agagtgcttt tggctggcag gaagcggcag gcggcgcggc gggatatacc
     8581 ttaagccagg cgattactaa cggttttcag cgcagtatgt tttccaatga ggtgggaatg
     8641 ggatcgacgc caaacgcggc agcggcagct gcatcctggc ctccgcatcc ggcagcacaa
     8701 gggattgtcc agatgattgg catttttatc gacaccctgg tcatctgtac ggcaagcgcc
     8761 atgctgatat tactggcggg taacggcaca acctacatgc cgctggaagg tattcagctt
     8821 atccagaagg cgatgcgggt gctaatgggt tcctagggtg ctgaatttgt taccctcgtg
     8881 gttattctgt ttgccttcag ctccatcgtt gccaactaca tttatgccga aaacaatctc
     8941 ttctttttac gcctgaacaa ccctaaagcg atctggtgtt tgcggatctg caccttcgca
     9001 acggtcatcg gcggcacctt gctaagcctt ccgctgatgt ggcaactggc agatatcata
     9061 atggcctgca tggctattac caatttgacc gccattttac tgctctcgcc tgtggttcat
     9121 accattgcca gtgattatct acgccagcgt aaactcggcg tgcgcccggt gtttgatccg
     9181 ttgcgttatc cggatatcgg tcgccagctt tctccggacg cgtgggatga tgtttcgcag
     9241 gagtaatcac aactatcgat caactcattc tctttttttg ctaaagtcgg cataaatttc
     9301 ctgcaaggac tggatatgct gattcttatt tcacctgcga aaacgcttga ttaccaaagc
     9361 ccgttgacca ccacgcgcta tacgctgccg gagctgttag acaattccca gcagttgatc
     9421 catgaagcgc ggaaactgac gcctccgcag attagcacgc tgatgcgcat cagcgacaaa
     9481 ctggcgggta tcaacgccgc tcgctttcat gactggcagc cagatttcac gccggcgaat
     9541 gcccgccagg cgattctggc gtttaaaggt gatgtctaca ccggcttgca ggccgaaacc
     9601 ttcagcgaag acgatttcga ttttgcccaa cagcatttgc gaatgctttc cggcttgtat
     9661 ggcgtactgc gcccgctcga tttaatgcag ccttatcgtc tggaaatggg gatccgtctt
     9721 gagaatgccc gagggaaaga tctgtatcaa ttctggggag atatcatcac caacaagctg
     9781 aacgaggcgc tcgcagcaca aggcgataat gtggtgatta atctggcgtc agatgagtat
     9841 tttaaatcgg tgaagccgaa gaaattgaat gccgagatta tcaagccggt gttcctcgat
     9901 gagaagaacg gcaaatttaa gatcatcagc ttctacgcta agaaagcacg cggtctgatg
     9961 agtcgtttca ttattgaaaa tcggctgacc aaaccagagc aactgactgg ttttaatagc
    10021 gaaggttact tctttgatga agattcctcc agcaatggcg aactggtgtt taaacgctac
    10081 gagcagcgtt aagtttgtag tcaatgccgg atgcggcgtg aacgccttat ccggcctaca
    10141 aaagcatgca gattcaatat attgcagaat taacgtaggc ctgataagac gcgccagcgt
    10201 cgcatcaggc gctgaatgcc ggattgttac ccggcatttg tcatttagcg gtgatgtttg
    10261 cctggaccat gaccgccgtg atgatcatga ggagctttct tatggtggcg cggcggtggc
    10321 ggcggtccgt gtgggtgcca gcgattgcct cgccattcat aatgttgttt ccaccagccg
    10381 tggtcgcgcc agtgacctcc atcccagtaa tagccacgat tatcacgatc gcctatctgt
    10441 aattttactg acgggactaa cgtaatttcc gcagcctgtg ctgccatggg agcgaccaga
    10501 accagggaaa gtgcgagtac gatagattgc atctttttca caggttactc cttttatcat
    10561 tgcccgttat gggtcgatat ccggagatta cgcgatgaga acgacggttg ttattctccg
    10621 caatcctaat ctctaagtta ccgcatttat tgataatttc tcctttttcc ctgtcgtttt
    10681 tctccataat ttcttcataa aaaagccggg ctgcataaaa gcaaacccgg cctgattgag
    10741 ataatgaata gattttactg atgattcatc atcaatttac gcaacgcaga aaaatcggcg
    10801 ggcagattat gtgaaagcaa gggtaaatca gcacgttctg ccagctcttt tggcagatcc
    10861 aacgtttcac cgagaatcgc ttccacgctc tctttaaatt tcgccggatg cgcggtgccg
    10921 aggaacaagc catattcgcc tggattcaac tggtcacgca gcgcacgata cgctacggca
    10981 gcgtgcggct ccgaggtgta gcccagttct tttaactcac gcattgtctg ttgcgtggtt
    11041 tcatcatcca cggctgcata acccagctct ttcagttgcc agattttgcg gcggaacaac
    11101 tcttccacac gcggccagtt gttcggctga ctcacgtcca tcgcgttgga taacgtcgcc
    11161 tgagtcgctt tgggtgacca ctgaccgtcg tgcaggaaac gtggcacggt atcgttcacg
    11221 ttggtcgcag caataaaacg tttcaccggc agaccgaatg acttcgccag cagacccgcc
    11281 gtcaaatcgc cgaagtttcc gcttggcacc gagacaacca gctggttgcg cgcctcctgc
    11341 ggcagctgcg caacagcttc aaagtagtag caaatctgcg ccagcaaacg gctgatgtta
    11401 atcgagttag ccgagtttaa ccccagcgcc actttcagtt cttcatcatc aaacgcctgc
    11461 ttcaccagcg cctgacaggc atcgaaatcg ccgtcgatgg caacagtttc gatattgccg
    11521 cccaacgtac agaacagttt ttcttgcagt ggactgattt tgcctcgtgg atagaggata
    11581 accactttca cattcggcaa accgtagaaa gcatgagcca ctgcagctcc ggtatcaccg
    11641 gaggtcgcgg tcagaatggt cactggctta tcatccgcaa tatgggtcag catttgtgcc
    11701 ataaagcgac cgccgaaatc tttaaatgcc agcgttggcc cgtggaacaa ttccagacaa
    11761 ccgacatcgc tttcaacatt ggcgaccgga gccgggaagg caaacgccgc gcgcacgcgc
    11821 tcttccagga tttcctgtgg gatttcatca ccaataaacg ccgagaggat cttcgcactg
    11881 cgggtgacaa aatccagctt cagcatctca tcaatttcag tcaggctgaa ttccggcagg
    11941 tcgtgcggaa aaaacagccc ctgatttttg cccaacccct gggttacggc ttgcgcaaag
    12001 ctgacctgct cgttgtgatc tttcagattg tagagtttca tttagttttc cagtactcgt
    12061 gcgcccgccg tatccagccg gcaaatatga acaaaacctt cctgattttg caggtagttc
    12121 ttacccaacc agtcggcaac gcgctgggcg gtatccggct tgtcacatag agcgaacaag
    12181 gtcgggccgg agccggagat accgctcgct accgcgccga tttccgcgac cgcctgccgc
    12241 gcctgccgga agccaggcag taaccgttca cggtagggtt cagcgataac atctttcatc
    12301 agcttcgcgg caagctcagg ctgacgggaa tagcaggcgt gaatgaagcc agccagatgt
    12361 cgcccgtgcg caatgcaatc ctggcggcga tactgcgccg gtaaaatagc ccgggcttct
    12421 gccgtcgaga ctttaattcc cggatacgcc agcacccaca gccactcatc aaaccctggc
    12481 acttgctggc tgatgatgtc gttttcttcg atcatcaact gcataccacc aagaaaacac
    12541 ggtgccacgt tgtcgtaatg aatgctgccg gagatacgtc cttccagctc gcccatcaaa
    12601 gccagcaaac gagtgtcatt aagcggcttg ccgcagtgtt cattcatcgc catcagcgcc
    12661 gcgaccaccg aacaggcgct ggagcctaag cccgaaccga tcggcatatt cttttccaga
    12721 gtcatcgcca ctggaatttg cttgccaagc tcctggcaaa aacgctccca gcactgataa
    12781 acgatatttt cccgtggttc tgacggcagc ttatcggcaa agcgtccgag tttgttgaga
    12841 ctgaatgtct ctgccgcctc aaccgtgact acatctccga gcaatgcacc atcaacgggt
    12901 gccaccgccg ccccgagcac atcaaacccg acgctcatat tggcactgga agccggggca
    12961 taaactttaa ccatgtcaga ctcctaactt ccatgagagg gtacgtagca gatcggcaaa
    13021 gacaccggca gcggtaacgt cattgcccgc accatatccg cgcagcacca acggcagcgg
    13081 ctgataatag tggctataaa aggccagggc gttttcgcca tttttcactt tgaacagcgg
    13141 atcattccca tccacttcgg caatcttcac gcggcagaca ccatcttcat caatattgcc
    13201 aacatagcgc aaaacttttc cttcatcacg ggccttcgcc acgcgcgcgg caaagagatc
    13261 gtcgagctgt gacagattcg ccataaaagc ggcaacatca ccctcggcgt taaactctgc
    13321 gggcagcaca ggttcaattt caatatccgc cagctccagt tcacgtcccg tttcacgagc
    13381 gagaatcaat agtttacgcg ccacatccat accagaaaga tcatctcgcg ggtccggttc
    13441 ggtataaccc atttcccgcg ccagcgtggt cgcctcggag aaactcatgc cttcgtctaa
    13501 cttgccgaag atataagaaa gcgaacctga aagaatgccg gagaacttca ccaattcatc
    13561 acctgcattg agcagatttt gcaggttctc aataaccggt aatccagccc caacgttggt
    13621 gtcatagagg aatttacgcc gcgatttttc cgccgcataa cgcaactgat gatagtaatc
    13681 catcgacgag gtgttggcct ttttgttcgg cgtgacaacg tggaaacctt cgcgcaggaa
    13741 gtcggcatat tgatccgcca ctgcctggct ggaggtgcag tcaacaatga ccgggttcag
    13801 cagatgatat tctttcacga ggcgaattaa gcgcccgaga ttaaacggct ctttggcttg
    13861 tgcaagttct tcctgccagt tttccagatt aaggccatgc acactagtga gcagagcctt
    13921 cgagttggca acgccgcaga cacgtaagtc gatatgttta ttcttcagcc agctttgctg
    13981 acgcttcagt tgctccagca gcgcaccgcc aacgccaccg acgccaatca caaacacttc
    14041 gataacctga tcggtattga acagcatctg atgagtaacg cgcacgccag tggtcgcatc
    14101 atcgttattt accacgacag agattgagcg ttcagaagat ccctgagcaa tggcgacaat
    14161 gttgatattg gcgcgggcca gcgcggcaaa gaatttcgcc gagatcccac gcaaggtgcg
    14221 cataccatca cctaccaccg agataatggc cagccgttcc gtcaccgcca gcggctccag
    14281 taagccttct ttcagttcca ggtagaactc ttcctgcatt gcccgttcag ctcgcacaca
    14341 gtcgctttgt ggaacgcaga aactgatact gtattcggaa gatgattgcg taatcagcac
    14401 cacggaaata cgggcgcgtg acattgcagc aaagacgcgc gccgccatgc caaccatccc
    14461 tttcatcccc gggccggaaa cgctgaacat tgccatgtta ttcagattgg aaatgccctt
    14521 gaccggtaat tcgtcttcat cacggctggc accaatgagc gtacctggtg cttgaggatt
    14581 tccggtattt ttaatcaggc aagggatctg gaactgggcg atgggggtaa tggtgcgggg
    14641 gtgaagaact ttagcgccga agtaggaaag ctccatcgct tcctggtagg acatcgactt
    14701 caacaacctc gcatcgggca cctgacgcgg gtcgcaggta tagaccccgt caacgtccgt
    14761 ccaaatctcg caacaatcgg cgcgtaaaca ggcagccagc accgccgcgg agtagtcgga
    14821 accgttacgt ccaagcacca ccagttcgcc tttttcatta ccggcggtga aacctgccat
    14881 caacaccatg tgatcagccg gaatgcggct tgccgcaata cggcgggtgg actcagcaat
    14941 atcgacggta gattcgaggt aatgccccac tgccagcagt ttttcgaccg gatcgataac
    15001 ggtaacgttg tgaccacgcg cttctaacac gccggccata atggcgatcg acattttctc
    15061 gccacggcaa atcagcgcag cgttgatgct atccgggcac tgccccaaca aactaatgcc
    15121 atgcaggaca tgttttattt gggcaaattc ctgatcgacg aaagttttca attgcgccag
    15181 cgggaacccc ggctgggcgg cggcgagtcc cgtcaaaagt tcggcaaaaa tacgttcggc
    15241 atcgctgata ttgggtaaag catcctggcc gctaatggtt ttttcaatca tcgccaccag
    15301 gtggttggtg attttggcgg gggcagagag gacggtggcc acctgcccct gcctggcatt
    15361 gctttccaga atatcggcaa cacgcagaaa acgttctgca tttgccactg atgtaccgcc
    15421 gaacttcaac actcgcatgg ttgttacctc gttacctttg gtcgaaaaaa aagcccgcac
    15481 tgtcaggtgc gggctttttt ctgtgtttcc tgtacgcgtc agcccgcacc gttacctgtg
    15541 gtaatggtga tggtggtggt aatggtggtg ctaatgcgtt tcatggatgt tgtgtactct
    15601 gtaattttta tctgtttgtg cgctatgcct atattggtta aagtatttag tgacctaagt
    15661 caataaaatt ttaatttact cacggcaggt aaccagttca gaagctgcta tcagacactc
    15721 tttttttaat ccacacagag acatattgcc cgttgcagtc agaatgaaaa gctgaaaaat
    15781 acttactaag gcgtttttta tttggtgata tttttttcaa tatcatgcag caaacggtgc
    15841 aacattgccg tgtctcgttg ctctaaaagc cccaggcgtt gctgtaacca gtcgaccagt
    15901 tttatgtcat ctgccactgc cagagtcgtc agcaatgcca tgactcgttc gcgtaaagct
    15961 tgcagttgat gttggtctgc cgttgtgtca cttttcgccg gttgttgtat taatgttgct
    16021 aattgatagc aatagaccat caccgcctgc cccagattga gcgaaggata atccgccacc
    16081 atcggcacac cagtaagcac gtcagccaac gctaactctt cgttagtcag cccggaatct
    16141 tcgcgaccaa acaccagcgc ggcatggctc atccatgaag atttttcctc taacagcggc
    16201 accagttcta ctggcgtggc gtagtaatga tatttcgccc gactacgcgc agtggtggcg
    16261 acagtgaaat cgacatcgtg taacgattca gccagtgtcg ggaaaacttt aatattatca
    16321 ataatatcac cagatccatg tgcgacccag cgggtggctg gctccaggtg tgcctgacta
    16381 tcgacaatcc gcagttcgct aaaacccatc gttttcattg cccgcgccgc tgccccaata
    16441 ttttctgctc tggcgggtgc gaccaggata atcgttatac gcatattgcc actcttcttg
    16501 accaaataac cgcgaacagg gtgatcactg tcaacttatt acgcggtgcg aatttacaaa
    16561 ttcttaacgt aagccgcaga aaaagccctt tacttagctt aaaaa
//
LOCUS       DAZPAB010000080        16507 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZPAB010000080 DAZPAB010000000
VERSION     DAZPAB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16507
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.080"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(74..880)
                     /gene="trpA"
                     /locus_tag="ACHUPG_002269"
     CDS             complement(74..880)
                     /gene="trpA"
                     /locus_tag="ACHUPG_002269"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR0861871.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(880..2073)
                     /gene="trpB"
                     /locus_tag="ACHUPG_002270"
     CDS             complement(880..2073)
                     /gene="trpB"
                     /locus_tag="ACHUPG_002270"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR0861872.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2085..3446)
                     /gene="trpCF"
                     /locus_tag="ACHUPG_002271"
     CDS             complement(2085..3446)
                     /gene="trpCF"
                     /locus_tag="ACHUPG_002271"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR0861873.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3447..5042)
                     /gene="trpD"
                     /locus_tag="ACHUPG_002272"
     CDS             complement(3447..5042)
                     /gene="trpD"
                     /locus_tag="ACHUPG_002272"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR0861874.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5042..6604)
                     /gene="trpE"
                     /locus_tag="ACHUPG_002273"
     CDS             complement(5042..6604)
                     /gene="trpE"
                     /locus_tag="ACHUPG_002273"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR0861875.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6626..6719)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6696..6740)
                     /gene="trpL"
                     /locus_tag="ACHUPG_002274"
     CDS             complement(6696..6740)
                     /gene="trpL"
                     /locus_tag="ACHUPG_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR0861876.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6878..7759
                     /gene="rnm"
                     /locus_tag="ACHUPG_002275"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6878..7759
                     /gene="rnm"
                     /locus_tag="ACHUPG_002275"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR0861877.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7756..8376
                     /locus_tag="ACHUPG_002276"
     CDS             7756..8376
                     /locus_tag="ACHUPG_002276"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR0861878.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8477..9349
                     /gene="rluB"
                     /locus_tag="ACHUPG_002277"
     CDS             8477..9349
                     /gene="rluB"
                     /locus_tag="ACHUPG_002277"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR0861879.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(9389..9979)
                     /gene="cobO"
                     /locus_tag="ACHUPG_002278"
     CDS             complement(9389..9979)
                     /gene="cobO"
                     /locus_tag="ACHUPG_002278"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFR0861880.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(9976..10734)
                     /locus_tag="ACHUPG_002279"
     CDS             complement(9976..10734)
                     /locus_tag="ACHUPG_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR0861881.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            10954..12003
                     /gene="sohB"
                     /locus_tag="ACHUPG_002280"
     CDS             10954..12003
                     /gene="sohB"
                     /locus_tag="ACHUPG_002280"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR0861882.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(12039..12290)
                     /locus_tag="ACHUPG_002281"
     CDS             complement(12039..12290)
                     /locus_tag="ACHUPG_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR0861883.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            12670..15267
                     /gene="topA"
                     /locus_tag="ACHUPG_002282"
     CDS             12670..15267
                     /gene="topA"
                     /locus_tag="ACHUPG_002282"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR0861884.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            15477..16451
                     /gene="cysB"
                     /locus_tag="ACHUPG_002283"
     CDS             15477..16451
                     /gene="cysB"
                     /locus_tag="ACHUPG_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR0861885.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT         4019 a         4195 c         4386 g         3907 t
ORIGIN      
        1 aaatgaattt aggatttttc cggcttcatt aaagaaagtt aaaatgccgc cagcggaact
       61 ggcggctgtg ggattaactg cgcgtcgccg ctttcatcgg ttgtacaaaa actttcagtg
      121 ccgccagcat tttctctggc tcattaatat gttgctcgat gattttaaca atggccgaac
      181 cagaaatcgc gcccgcagct cctgcatcaa tcgctgcttt tacctgatcc ggggcggaaa
      241 taccaaaccc ctgcaatgga ggtgcagcgt tgtactcttt cagcttcgca accagatgat
      301 tgaggggtaa cgcggcgcgg ttttctgcgc cggtcacgcc cgctcgtgac agcaaatatg
      361 tgtaaccacg accgtaagag gctatctggc gcagcaggtc gtcatcggca tttggcgggc
      421 agatgaagat aggtgcgaca ttatggcgca acgcggcctg gcggaagggc gcggactctt
      481 caactggcac atcggcaacc agcaccgaat cgacgccgac tttctcgcac tgggcataaa
      541 actcatcaat gcctttgtta aacaccagat tggcatacat caacaggcca atgggaatgg
      601 tcgggtgttt ctggcgaatc agtgccagca tttcaaaaca ttgtgccgga gtcacacctg
      661 ccgcaaaggc gcgcagagtg gcgttttgaa tggtcgggcc atccgccagt gggtcggaga
      721 aggggatacc taactccagc gcgtcagcac cggcttcaat tagcgtatcg ataattttca
      781 atgactgctc aatgcccgga tcaccgagcg tgacgaaagg aacgaatgcg ccttctttgc
      841 gctccttcaa ctgggcaaac agagattcgt agcgttccat cagatttccc ctcgtgcttt
      901 caaaatatcg tgaacggtga agatgtcttt atcgccgcga ccggaaaggt taaccaccag
      961 tagctgctct ttatccgggt tttcgcgcat cattttcagc gcatgggcca gggcgtggga
     1021 ggattccagc gccgggatga tcccttcgtg caggcacagc gttttgaagg cttcaagggc
     1081 ttcatcatcg gtaatagaca cgtaatcagc gcgtccagtg ctgttaagat acgcgtgttg
     1141 tggaccgaca gacgggaaat ccagtccggc ggagatggag taagattctt caatctgccc
     1201 gtcttcggtt tgcatcatcg gcgctttcat accgaaataa atgcccacgc gaccatgttt
     1261 tagcggtgcg ccgtgctcgc cagtttcgat accgtgacca ccaggctcca caccaatcag
     1321 gccgacgttg gtttcattga tgaaatcagc aaacatgccg atggcattcg aaccgccgcc
     1381 aacacaggcg ataacggcat ccggcaggcg accttctctt tccagaatct gcgctttggt
     1441 ttcttcgcca atcatccgct gaaactcacg gacaatggtc ggataaggat gcgggccagc
     1501 cgcggtgccc agcatatagt gcgcggtttc ataactgccg gaccagtcgc gtagcgcctc
     1561 attacaggca tctttcagcg tggcggaacc gctatgtacc gggatcactt ccgcacccat
     1621 taagcgcata cgaaaaacgt taggcgactg gcgttcaacg tctttggcac ccatataaat
     1681 acggcatttc aggccgagca gggcgctggc aagggccgac gccacgccat gctgaccggc
     1741 cccggtttcg gcgatgattt cggttttacc catccgcttc gccagcaacg cctgccccag
     1801 cacctggttg gttttatgtg cgccgccgtg cagcaaatct tcacgcttga gatacagcgt
     1861 ggtgttcgtc ccggctgtaa tgttctggca tttggtcagt gcggttggac gcccggcata
     1921 gtttttcagc aggtcgttga actgggcttg aaattcagga tctttttgcg cgctgacaaa
     1981 agcttcttcc agctggcgca gagcaggcat caggatttgt ggcacgtaca tgccgccaaa
     2041 ctcaccaaaa taggggttaa gtaatgttgt cattgttcct ttccttaata tgcgcgcagc
     2101 gtctggaaaa ccgaggccaa aagacgtgcg tctttgatgc ccggttgcga ctctacagca
     2161 gaattaaaat caagtccggc gcagccggtt tgtgccgctt ccacgcagtt atctgcgcct
     2221 aagccccccg ccagcagaac gttgccaagc gattgaccat ttaatagtga ccagtcaaaa
     2281 cgttgcccgc ttccaccctg gccgttgtct aaaacatatt tatcaacgtg ctgaaactcg
     2341 cgggcgggca gggtttcacc gacgcttaat gctttccaga tggcaacatg tgctggcaga
     2401 gcttcacgca gcgtatcgat atacagctga tcttcattgc catgcagttg cactgccgcc
     2461 agcgataaca ccttagcttt gtccaccaca tcggcaatat cgtgattgcg gaacacgcca
     2521 acatactgca acggtgccgc agccatcact tcctgcgcct gttcaacgtt gacgcaacgc
     2581 ggtgatgtcg caacaaaaat caacccaccg taaatcgcgc ccgcgtcata agctgcttta
     2641 gcatcttgcc cacgcgtcag gccacatact ttattctcac ccagcaacac ccggcgcacg
     2701 gcggcgtgca aatcgtcatg ggccatcaac gccgaaccaa tcagaaaacc gttagcgaag
     2761 tggcttaact cgcgcacctg agcgtaagta ttgatgccgg attcgctgat taccgttacg
     2821 ttgtgcccca gtttcggcgc aagctcgcgg gtacggttga gatcaatcga caaatcacgc
     2881 agatcgcggt tgttgatgcc aacgaccttt gcccctaatg caatggcgcg ctccagttcc
     2941 tcttcattac tgacttcggt cagcaccccc atctccagac tgtgagcgac ggctgcaagc
     3001 tggcgatatt ggtcgtcatc cagtactgaa agcattaata agcaggcatc ggcctggtaa
     3061 tagcgcgcca gatagatctg gtaagggtcg ataatgaagt ctttacataa aatcggctgc
     3121 ggggcgattt ggctgacgat gaggaggaaa tcaaagctcc cctgaaaata tttctcatca
     3181 gtcagcaccg aaattgccga agcgtaatgt ttataaatgg cggcaatgcg tgccggatcg
     3241 aaatcatcac ggatcacgcc ttttgacggc gacgcttttt tacactccag aataaacgcc
     3301 gtgcgtgcac cctgtagcgc atcataaaaa tgtcgtgtgc tcggctgaac ctcattctga
     3361 aaactggcca gcggttgctg ctgtttgcgg gtttctaccc aaatcgcctt gtctgcgacg
     3421 attttcgcta aaacggtttg catcatttac cctcgtgccg ccagcgcggt aactctgtcg
     3481 taagcggaac cactgcgcag tacctcaaga acggtttgcg cattggcttg cagatcttca
     3541 tggccatgca ggcgcattaa catggcgacg ttcgcagcga cggctgcttc atgggcggcg
     3601 tcgcctttac cttgtaacaa gcgtgttaaa atgtcacggt tttcttcagg tgttccgcct
     3661 gccagttgct cctggtggta gggagtcagg ccaaaatctt cagcggtcaa ttgatagctc
     3721 ttaattttgc cgtcatgcag ctcggcaacg attgtcggcg cgtgtaatga aacttcatcc
     3781 atcccgccgc tgtgcaccac cgccgcgcgt tgatacccca gcacgcgcaa ggtttcggca
     3841 atcggcagca ccagttccgg actataaaca ccaattaacg ccagcggcgg atgcgccggg
     3901 ttaatcaatg gccccagcac attgaacagg gtgcgggttt tcagttgctg gcgaaccggc
     3961 atcgcgtggc ggaatccggt gtgatatttc ggcgcaaaga ggaaacatac gcctaactcg
     4021 tccagcgcct ggcgcgattt attggcgttc atatcaagat taataccgaa tgccgccagc
     4081 agatccgacg agccggattt actggagacg ctgcggttac cgtgtttcgc cactttcagc
     4141 ccacaggccg cggcgacaaa cgcactggcg gtagaaatat tgatactgtt gctgccgtca
     4201 ccgccagtac cgacgatatc agcaaacaga taatccgggc gcgggaacgg cgctgcgttt
     4261 tccagtagcg cggttgctgc cccggcgatc tcgttcgggt gctcaccgcg aattttcatg
     4321 ctcaccagcg ccgccgccag ttgttccggc ttcagctcgc cacgcaccac cgctgaaaac
     4381 agctggtggc tttcttgttg gctaagcgtc tgcgcctgat acagtttttc cagaatcggt
     4441 tgcagcgtgt tggctggctc tagtttctgc tgcgcccagg ccagcgtttg ttccagcagg
     4501 cgagcgccct gggtggtgag aatggattcc ggatggaact ggaatccaca aacgcgatcc
     4561 gcatcgtgac gtactgccat caccatgcca ttaaaatggg cgttgatggt taaaccggcc
     4621 ggaatgttac tgccaaccag cgagtgataa cgcgccaccg gcagcgggtt tgttaatccg
     4681 gcaaacatcg cctgaccgtc atgttcaatg ctcgacgctt taccgtgaag aatttcgccc
     4741 gcctgaccga catagccccc gtaagcttcg acaattgcct gatgtccgag gcaaatgcca
     4801 ataatgggca gcttgccacg caagcgggtg aggagttccg gcatacaacc ggcttcgctc
     4861 ggcacaccgg ggccaggaga aagcatcagt accggattgc tcatcgtcgc caggcgttca
     4921 attaaggtct gcgccggaat atggttgcgg taaatcacca cgttatgacc attgctgcgc
     4981 aactgatctg ccaggttgta cgtaaaagag tcgatattat cgagcagcag aatgtcagcc
     5041 atcagaaagt ctcctgtgca tgatgcgcgg tggcaatagc gcgcagtaca gcgcgggctt
     5101 tgttacgggt ttcgtcggct tccgactgcg gaacagaatc aaggactaca ccagcacccg
     5161 cttgcacggt ggcgataccg ttttccacca gcgccgagcg gatcacaatg caggtgtcga
     5221 gatcgccatg cgcggtgaaa taacctaccg cgccgccgta gctgccgcgg cgacgacctt
     5281 ccgcctcggc aattaactgc atagcgcgta ctttcggcgc accgcttaac gtccccatat
     5341 tcatacaggc gcgataagcg tgcagggcgt caagatcgtg acgcagttcg ccgactacgc
     5401 gagagacgag gtgcatcaca taggaataac ggtcaacttt ggtgagatcg gcgatgtagc
     5461 ggctgccagg ggtgcaaatg cgtgccagat cattacgggc gagatcaacc agcatcagat
     5521 gttcagacag ctctttatgg tcagtacgca tttccagttc aatacggctg tcgagatctc
     5581 tgtccagtga accatcggcg cgacgaccgc gtgggcgtgt tccggcaatc gggtagatct
     5641 caatctggcg gctggtggca tcatacttga gcgagctttc cggcgacgcg ccaaataggg
     5701 tgaaatcatt atcctgcata aaaaacatgt acgggctggg attactcttt ttcagcacgt
     5761 aataggccgc cagcggtgac gggcagggca gagagaaacg gcgagatggc accacctgga
     5821 aaatttctcc agcgcgaatc gctttttgca acaaacgcac tacgccaccg aactcttcat
     5881 cgctctgatt acattcacaa cgcatatgcg gcacggaaac cactggcagc ggcggcgcgg
     5941 cttcggtcag ttgctgacgt agttcgttca ggcgagcagt gagacgttgt ttttcttctt
     6001 cattcggagc aaacaggctg gcctgaatac gggtgctttt tttctgatgg tcaatcacca
     6061 tcagcgtttc agcgagataa aaacagaaat cagggcagtt attttccgct gacagttgcg
     6121 gtaaatcttc aaatcccgcc acaaggtcat aagagaacag gccgccgaag aacattgctt
     6181 ctcgttcttc cttcggtaca ttcaacagat tctgcaataa acggaaagcg tcaaaaaccg
     6241 aaagggagca taagcgggcg tcttcatcca gcagtggact gacagggggg aagcgcagca
     6301 cacggcagtt tggtgattgt tcactttcca cacccgcagg cagggcgtta tccagtagtg
     6361 ccagcagggc ttcgccattg ccggaaagtg cctgaattgt gacggtgtca cctaatgctg
     6421 taatgcgcag cgcactgtct atcagcagca ggctttttaa atcatctttg ctgtcgatat
     6481 ctgcggattc cagcagcagc gttgccggac gattcccaca caactggtga aaaagcgcag
     6541 tcgggttgtc gcgataagcg ccttcgcagg ttagcagttc gagagtcggt ttttgtgttt
     6601 gcattgttat tctctaattt tgttcaaaaa aaagcccgct cattaggcgg gctgggtatc
     6661 tgattgcttt acgcatggtg aatacactgc ccgtttcagg aagtgcgcca ccaacctttc
     6721 agtacgaaaa ttgctttcat tgtcgatacc ctttttacgt gaacttgcgt actagttaac
     6781 tggttcgatg attaattgtc aacagctcat ttcagaatat ttgccagacc cgttatgatg
     6841 tcggcgcaaa aaacattatc cagaacggga gtgcgccttg agcgacacga attatgcagt
     6901 gatttacgac ctgcacagcc ataccacagc ttccgatggc tgcctgacgc cagaagcatt
     6961 ggtgcaccgt gcagtcgaga tgcgcgtcgg caccctggcg atcaccgacc atgacaccac
     7021 agctgcaatc gcgccagcaa gagaggaaat ttcacgttcg ggactggcgc tgaatcttat
     7081 tcccggcgtg gaaatttcca cggtctggga aaatcatgaa attcatattg tcgggctgaa
     7141 tattgatatt actcatccgc taatgtgtga gttcctcgcg cagcagacag aacggcgcaa
     7201 tcagcgggcg cagcaaattg ccgaaagact tgaaaaagcg caaatacctg gggcgctgga
     7261 aggcgcacaa cgactggcgc agggcggcgc agtgacgcgc ggtcattttg cgcgtttcct
     7321 tgtggagtgc ggcaaagcca gttcaatggc agatgtcttt aaaaagtatc tggcgcgcgg
     7381 gaaaaccgga tacgttccgc cacagtggtg tacaatagaa caagctattg atgtcattca
     7441 tcattctggc ggtaaggcgg tgctagctca tcctgggcgg tacaatctta gtgctaaatg
     7501 gctgaaaaga ttggttgcgc attttgccga acaccacggc gacgcgatgg aagtcgcgca
     7561 gtgccagcaa tcgcccaatg aacgcaccca gctggccaca cttgcgcgtc agcatcattt
     7621 atgggcatca caaggatctg attttcatca gccatgcccg tggatcgaac tgggtcgtaa
     7681 actctggctg cccgcaggcg ttgaaggcgt ctggcagcta tgggaacagc cgcagaacac
     7741 cacagagagg gaattatgag ccagtttttt tatattcatc ctgataaccc gcagcaacgt
     7801 ctgatcaacc aggcggtgga gatcgtgcgt aaaggcgggg tgattgtcta tccaactgat
     7861 tccggctatg cgctcggctg taaaattgaa gataaaaacg ccatggagcg tatttgtcgt
     7921 attcgccagc tgccggacgg tcacaacttt accctgatgt gtcgcgatct ttctgaactc
     7981 tcgacctatt cattcgtgga taacgttgcg tttcgtttga tgaaaaacaa cacgccgggc
     8041 aactatacct tcatcctgaa ggggacgaag gaagtgccac gccgtctgtt gcaggagaaa
     8101 cgcaaaacca tcggtatgcg tgtgccttct aacccgattg cgcaggcgtt gcttgaagca
     8161 ctgggcgaac cgatgctttc cacttcgcta atgctgccag gcagcgaatt taccgaatcc
     8221 gatcccgaag aaattaaaga ccgtctggaa aaacaggtag atttgattat tcacggcggc
     8281 tatctcgggc agaaaccaac aacggttatt gatcttaccg atgatacgcc ggtcgtggtg
     8341 cgtgaaggcg taggtgatgt gaagcctttc ttataagtgc gtaactctgt atactacgcg
     8401 gccttaaaat ggcgctatac ggcgccattg attctcctgt tcgacgcctg tgaaggcgac
     8461 accaaaagga agctctatga gcgaaaaatt acagaaagtg ctggcccgcg ctggccacgg
     8521 ctcccgccgt gaaatcgaat ctattattga agccggtcgt gtgagtgttg atggcaaaat
     8581 tgctaaactc ggcgatcgcg ttgaagttac ccctgggctg aaaattcgta tcgatggtca
     8641 tctgatttcg gtacgtgagt ccgctgaaca aatttgtcgc gttctggcct attacaaacc
     8701 ggaaggtgag ttgtgtaccc gtaacgatcc ggaagggcgt ccgacggtgt ttgaccgtct
     8761 gccaaagctg cgtggcgcac gctggattgc cgtaggtcgt ctggacgtta acacctgtgg
     8821 tctgctgctg ttcaccaccg atggtgaact ggcaaaccgt ttaatgcatc caagccgtga
     8881 agttgaacgt gaatacgccg tgcgtgtatt tggtcaggtt gacgacgcga aactgcgtga
     8941 tttgagtcgt ggcgtgcaac tggaagatgg tccggcagct tttaaaacca tcaagttcag
     9001 cggcggcgaa gggatcaacc agtggtacaa cgtgactctg accgaaggcc gtaaccgtga
     9061 agttcgtcgt ctgtgggaag cggttggtgt gcaggtgagc cgcctgatcc gtgttcgtta
     9121 cggtgatatc ccactgccaa aaggtctgcc acgcggtggc tggacggagc tggacctcgc
     9181 ccagactaac tatctgcgcg aactggtgga gcttccgcca gaaaccagct ctaaagtcgc
     9241 tgtagaaaaa gaccgtcgtc gcatgaaggc taatcagatt cgtcgtgcgg tgaaacgtca
     9301 cagtcaggtg agcggcagtc gtcgttctgg cgggcgtaat aacggttaat caaagtatgc
     9361 cggacgtcat atccggcatt tttacagatt aataatcaat ccctatttgc gccttaacac
     9421 cggcatcaaa cgcatgtctg atggggcgca gctcacttac cgtatccgcc agttcaagaa
     9481 tatcccgatg acagcctcga cccgtgataa tcaccgtctg ttgatgtgga cgttcattta
     9541 acgcctgcac cacttcttcc agtggcaaat agtcatacgc caccatatac gtcagttcat
     9601 caagcaaaac catatccagt gaggaatcag caagcatccg ctttgcatgt tgccagactt
     9661 cgcggcaggc ggcggtatca gactcgcggt tttgtgtatc ccaggtaaag cccgttgcca
     9721 tcacctgaaa ctcaacgcca tgtggctcca gcagattgcg ttcgccatta ggccaggtgc
     9781 ctttaataaa ctgcacgacg cccacttttt ttccgtgacc aactgcgcgt gttgccgtac
     9841 caaatgccgc ggtggttttg ccttttccat tgccggtaaa gacgatgata ataccgcgtt
     9901 catcctgggc ctgggccaca cgagcatcta ctttttcttt cactcgctgc tgacgctgtt
     9961 ggtagcgttc atcactcatt gggaaattcc tggtttacgg cccggttggg cgtcaaaggt
    10021 catgccggtt ttacggcggc tgtcatcgcc catcagccag aggtagagcg gcatgatatc
    10081 agcgggtgtt ttaagtttct gtggatcttc ggtcgggaag gcgcttgcgc gcattgcggt
    10141 gcgcgtaccg cctgggttaa tgcagttgac gcgcaggcgc tgctgatatt catcggccag
    10201 tacctgcatc atcccttcgg tggcaaattt cgacgctgca tatgcacccc agttggctcg
    10261 tccctgacgt ccaacgcttg atgaagtaaa gaccagtgaa ccggcgtccg atttgagtaa
    10321 taaaggaagc agtgcctggg tgagcataaa ggtggcatta acgttgatct gcatgacgtc
    10381 ctgccagacc tgcggatttt gttcgctcat tgggcaaaca tcgccgagca atccggcatt
    10441 atgcaaaaca ccatccagac gcggataatt aacgacaatg cgctgtgcca gttgttggca
    10501 attttcggac gtgcaggtca gcaaatcgag aataaaccac tgtggctgac gcccagtttc
    10561 ttcgtttatg tggctggcta cctgacgtaa tttttcttca ttacggccca acagaatcac
    10621 tgtcgcacca tagcgtgcat acgtcatcgc ggcttcacga ccaatcccat cgctggctcc
    10681 cgtcaccagg ataatgcgat cattgagtaa atcttgtttt ggctggtaat gcatggctac
    10741 tcctcaacga cgttgtctgt cgcgctgcca gtgtacttta tgactttggg gctttatgcc
    10801 tgaaacagaa gggcatttca atcagcctgg ggcgataaca ccgcaaaatg gatactttgt
    10861 catactttcg ctgcaataac atctctgcga gacggcttaa catgcctgtt gtaaactgtg
    10921 agccaaagcg ttgtttaacc aaggtgggga ctcgtggaat tgttgtctga atatggtttg
    10981 tttttggcta aaatcgttac cgttgtgcta gcgattgcgg cgattgccgc cattattgtc
    11041 aatgttgctc aacgtaataa acgccagcgt ggcgagttac gggtcaacaa tctcagcgaa
    11101 cagtataagg agatgaaaga agaactggcc gcggcgctga tggactcaca tcagcaaaaa
    11161 cagtggcaca aagcgcagaa gaaaaagcac aagcaagaag cgaaagcagc aaaagcgaaa
    11221 gccaaactgg gcgaggtggc aactgacagt aaacctcgcg tctgggtgct ggattttaaa
    11281 ggcagcatgg acgcccatga agtgaactcg ctacgtgaag agataacggc ggtactcgca
    11341 gcattcaaac cgcaggatca ggttgtgctc cgtctggaaa gccctggtgg catggtgcat
    11401 ggttacgggt tggcggcttc gcagctgcag cgtctgcgtg ataaaaacat tcctttaact
    11461 gttacggtag acaaagtcgc tgccagcggc ggttacatga tggcctgtgt ggcggacaaa
    11521 attgtttccg caccgtttgc tattgtgggt tccattgggg tggtggcgca aatgcccaac
    11581 tttaaccgct tcctgaaaag caaagatatt gatatcgaac tgcacaccgc cgggcagtat
    11641 aagcgcacgc tgaccttgct gggtgaaaat accgaagaag ggcgggagaa attccgcgaa
    11701 gagctgaacg aaacgcatca gttatttaaa gattttgtga agcgtatgcg tccgtctctg
    11761 gatattgaac aggtggcaac gggtgaacac tggtacggac aacaggcggt agagaaaggc
    11821 ctggttgatg aaatcaacac cagtgatgaa gttattctta gcctgatgga aggccgtgaa
    11881 gtggttaatg tacgctatat gcagcgtaaa cgactcattg accgattcac cggcagcgcg
    11941 gcagagagcg ccgatcgatt gttgctacgc tggtggcagc ggggtcaaaa gccattgatg
    12001 taaaagacaa acgcgaggct aagacctcgc gttttgcttt aatcaaccag atgatatttt
    12061 tctgaaagca catgggccag gtgtttgaac atattaaaca ccgcggtgct tttggctgtt
    12121 ggcaatcctt gttcatctaa aaagtagtcg ccggtaaata ccagcacgcc attacgctgc
    12181 gtgacgccgg tggcttcaat ccctgcgagc gtatcctcat gctcacgaat gattttgttg
    12241 gcctctttca acagcgtttc gcggtcgatg ggttgtgtct ctttgttcat tatttactcc
    12301 ttaaacaagg acattagtct acgccaggca tggcttgcag acaaatatac cacgctggtg
    12361 gcaagagcgc cttactggca actttggatt ttgcatgcta ataaagttgc gtatcggatt
    12421 ttatcaggta cagtgtgacg ctttcgtcaa tctggcaata gatttgcttg acattcgacc
    12481 aaaaatccgt cgtgctatag cgcctgtagg ccaagacctg ttaactcagt cacctgaatt
    12541 ttcgtgaaca gagtcacgac aaggggttga tatccgcaga gagcgagtcc atatcggtaa
    12601 ctcgttgcca gtggaaggtt tatcaacgtg cgacgcattc ctggaagaat caaattaggt
    12661 aaggtgaata tgggtaaagc tcttgtcatc gttgagtccc cggcaaaagc caaaacgatc
    12721 aacaagtatc tgggttgtga ctacgtggtg aaatccagcg tcggtcacat ccgcgatttg
    12781 ccgaccagtg gctcagctgc caaaaagagt gccgactcta cctccaccaa gacggctaaa
    12841 aagcctaaaa aggatgaacg tggcgctctc gtcaaccgta tgggggttga cccgtggcac
    12901 aattgggagg cgcactatga agtgttgcct ggtaaagaga aggtcgtctc tgaactgaaa
    12961 caactggctg aaaaagccga ccacatctat ctcgcaaccg accttgaccg cgaaggggaa
    13021 gccattgcat ggcacctgcg ggaagtgatt ggtggtgatg atgcgcgcta tagccgagtg
    13081 gtgtttaacg aaattactaa aaacgcgatc cgccaggcat ttaacaaacc gggtgagctg
    13141 aatattgatc gtgttaatgc ccagcaggcg cgtcgcttta tggaccgcgt ggtggggtat
    13201 atggtttcgc cgctgctgtg gaaaaagatc gctcgtggtc tgtctgccgg tcgtgtgcag
    13261 tcggtggcgg tccgcctggt ggtagagcgt gagcgtgaaa ttaaagcgtt cgtaccggaa
    13321 gagttctggg aagtcgatgc cagcacgact acgccatctg gtgaagcgtt ggagttgcag
    13381 gtgactcatc agaatgacaa accgttccgt ccggtcaaca aagaacaaac tcaggctgcg
    13441 gtaagtctgc tggaaaaagc gcgctacagc gtgctggaac gtgaagacaa accgacaacc
    13501 agtaaacctg gcgctccttt tattacctct acgctgcaac aagctgccag cacccgtctt
    13561 ggttttggcg tgaaaaaaac catgatgatg gcgcagcgtt tgtatgaagc aggctatatc
    13621 acttacatgc gtaccgactc cactaacctg agtcaggacg cggtaaatat ggttcgcggt
    13681 tatatcagcg ataattttgg taagaaatat ctgccggaaa gtccgaatca gtacgccagc
    13741 aaagaaaact cacaggaagc gcacgaagcg attcgtcctt ctgacgtcaa tgtgatggcg
    13801 gaatcgctga aggatatgga agcagatgcg cagaaactgt accagttaat ctggcgtcag
    13861 ttcgttgcct gccagatgac cccagcgaaa tatgactcca cgacgctgac cgttggtgcg
    13921 ggcgatttcc gcctgaaagc acgcggtcgt attttgcgct ttgatggctg gacaaaagtg
    13981 atgcctgcac tgcgtaaagg cgatgaagat cgtatcttac cggcagtcga taaaggcgat
    14041 gctctgacgc tcgttgaact aacaccagcc cagcacttta ccaagccgcc agcccgtttc
    14101 agtgaagcat cgctggttaa agaactggaa aaacgtggta tcggtcgtcc gtctacctat
    14161 gcgtcgatca tttcgaccat tcaggatcgt ggctatgtgc gagtagaaaa tcgtcgtttc
    14221 tatgcggaaa aaatgggcga aatcgtcacc gatcgccttg aagaaaattt ccgcgagtta
    14281 atgaactacg actttaccgc gcagatggaa aacagcctcg accaggtggc aaatcacgaa
    14341 gcagagtgga aagctgtact ggatcacttc ttctcggatt tcacccagca gttagataaa
    14401 gctgaaaaag atccggaaga gggtggtatg cgcccgaacc agatggttct gaccagcatt
    14461 gactgcccga cttgtggtcg caaaatgggg attcgcacag cgagtaccgg ggtattcctt
    14521 ggctgttctg gctatgcgct gccgccgaaa gagcgttgca aaaccaccat taacctggtg
    14581 ccggaaaacg aagtgctgaa cgtgctggaa ggcgaagatg ctgaaaccaa cgcgctgcgc
    14641 gcaaaacgtc gttgcccgaa atgcggcacg gcgatggaca gctatctcat cgatccgaaa
    14701 cgtaagttgc atgtctgtgg taataaccca acctgcgacg gttacgagat cgaagagggc
    14761 gaattccgca ttaaaggtta tgacggcccg atcgttgagt gtgaaaaatg tggctctgaa
    14821 atgcacctga aaatggggcg tttcggtaaa tacatggcct gcaccaacga agagtgtaaa
    14881 aacacacgta agattttacg taacggtgaa gtggcaccac cgaaagaaga tccggtgcca
    14941 ttacctgagc tgccgtgcga aaaatcagat gcttatttcg tgctgcgtga cggtgctgcc
    15001 ggtgtgttcc tggctgccaa cactttcccg aaatcgcgtg aaacgcgtgc gccactggtg
    15061 gaagagcttt atcgcttccg cgaccgtctg ccggaaaaac tgcgttatct ggccgatgcg
    15121 ccacagcagg atccggaagg taataagacc atggttcgct ttagccgtaa aaccaaacag
    15181 caatatgtct cttcggaaaa agacggaaag gcgactggct ggtcagcatt ttatgttgat
    15241 ggcaaatggg ttgaaggaaa aaaataacct ttaattctgt caggttttta taaacaaagg
    15301 gtcgcgaaag cggccctttt ttattgcata ttattttttc ttcacaccta tacactaagg
    15361 ctataaatga tatagtggtt atagttagca ccttttttat tattaaatcg tattagtcac
    15421 ccgccaggtg tgacgaaaaa acgatgttct gatggcgtct aagtggatgg tttaacatga
    15481 aattacaaca acttcgctat attgttgagg tggtcaatca taacctgaat gtctcatcaa
    15541 cagcggaagg actttacaca tcacaacccg ggatcagtaa acaagtcaga atgctggaag
    15601 acgagctagg cattcaaatt ttttcccgaa gcggcaagca cctgacgcag gtaacgccag
    15661 caggacaaga aataattcgt atcgctcgcg aagtcctgtc gaaagtcgat gccataaaat
    15721 cggttgccgg tgagcacacc tggccggata aaggctcgct gtatatcgcc accacgcata
    15781 cccaggcacg ctacgcgtta ccaaacgtta tcaaaggctt tattgagcgt tatccccgcg
    15841 tttctttgca tatgcaccag ggcccgccga cacaaattgc tgatgccgtc tctaaaggca
    15901 atgccgattt cgctatcgcc acagaagcgc tgcatctgta tgaagattta gtgatgttac
    15961 cgtgctacca ctggaatcgg gctattgtag tcactccgga tcacccgctg gcaggcaaaa
    16021 aagccattac cattgaagaa ctggcgcaat atccgttggt gacatatacc ttcggcttta
    16081 ccggacgttc agaactggat actgccttta accgcgcagg gttaacgccg cgtatcgttt
    16141 tcacggcaac ggatgctgac gtcattaaaa cttacgtccg gttagggctg ggggtagggg
    16201 tcattgccag catggcggtg gatccggtcg ccgatcccga ccttgtgcgc gttgatgctc
    16261 acgatatctt cagccacagt acaaccaaaa ttggttttcg ccgtagtact tttttgcgca
    16321 gttatatgta tgatttcatt cagcgttttg caccgcattt aacgcgtgat gtcgttgatg
    16381 cggctgtcgc attgcgctcc aatgaagaaa ttgaggtcat gtttaaagat ataaaactgc
    16441 cggaaaaata atttcttgcg ttattttcgg caccttttat gtagcgaagg tgccggaata
    16501 tattctc
//
LOCUS       DAZPAB010000081        15937 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZPAB010000081 DAZPAB010000000
VERSION     DAZPAB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.081"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            95..637
                     /locus_tag="ACHUPG_002284"
     CDS             95..637
                     /locus_tag="ACHUPG_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR0861886.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            724..1926
                     /locus_tag="ACHUPG_002285"
     CDS             724..1926
                     /locus_tag="ACHUPG_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR0861887.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            1990..2451
                     /locus_tag="ACHUPG_002286"
                     /pseudo
     CDS             1990..2451
                     /locus_tag="ACHUPG_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(2492..3442)
                     /locus_tag="ACHUPG_002287"
     CDS             complement(2492..3442)
                     /locus_tag="ACHUPG_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR0861888.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(3635..4825)
                     /gene="glpC"
                     /locus_tag="ACHUPG_002288"
     CDS             complement(3635..4825)
                     /gene="glpC"
                     /locus_tag="ACHUPG_002288"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR0861889.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(4822..6081)
                     /gene="glpB"
                     /locus_tag="ACHUPG_002289"
     CDS             complement(4822..6081)
                     /gene="glpB"
                     /locus_tag="ACHUPG_002289"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR0861890.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(6071..7699)
                     /gene="glpA"
                     /locus_tag="ACHUPG_002290"
     CDS             complement(6071..7699)
                     /gene="glpA"
                     /locus_tag="ACHUPG_002290"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR0861891.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            7972..9330
                     /gene="glpT"
                     /locus_tag="ACHUPG_002291"
     CDS             7972..9330
                     /gene="glpT"
                     /locus_tag="ACHUPG_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR0861892.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            9335..10411
                     /gene="glpQ"
                     /locus_tag="ACHUPG_002292"
     CDS             9335..10411
                     /gene="glpQ"
                     /locus_tag="ACHUPG_002292"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR0861893.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(10453..10695)
                     /locus_tag="ACHUPG_002293"
                     /pseudo
     CDS             complement(10453..10695)
                     /locus_tag="ACHUPG_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            10690..10794
                     /locus_tag="ACHUPG_002294"
                     /pseudo
     CDS             10690..10794
                     /locus_tag="ACHUPG_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10874..11524
                     /gene="inaA"
                     /locus_tag="ACHUPG_002295"
     CDS             10874..11524
                     /gene="inaA"
                     /locus_tag="ACHUPG_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFR0861894.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(11578..11832)
                     /gene="yfaE"
                     /locus_tag="ACHUPG_002296"
     CDS             complement(11578..11832)
                     /gene="yfaE"
                     /locus_tag="ACHUPG_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR0861895.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(11832..12962)
                     /gene="nrdB"
                     /locus_tag="ACHUPG_002297"
     CDS             complement(11832..12962)
                     /gene="nrdB"
                     /locus_tag="ACHUPG_002297"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR0861896.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(13196..15481)
                     /gene="nrdA"
                     /locus_tag="ACHUPG_002298"
     CDS             complement(13196..15481)
                     /gene="nrdA"
                     /locus_tag="ACHUPG_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR0861897.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPNIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            15609..15752
                     /gene="ypaB"
                     /locus_tag="ACHUPG_002299"
     CDS             15609..15752
                     /gene="ypaB"
                     /locus_tag="ACHUPG_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR0861898.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         3698 a         3933 c         4344 g         3962 t
ORIGIN      
        1 agatcccttt tttcgctcat tgaatcctct gtgccaggca gtacaatccc gcagctttgg
       61 gaattttgat taattgcttg tgggaaaggg gataatgaaa aaaattgcgc tagcaggtct
      121 ggccggaatg ctgttggttt ctgcatcggt caatgcaatg agcatcagcg gccaggcggg
      181 taaagaatac accaatattg gtgtcggttt tgggactgaa tcgacgggcc tggctttaag
      241 cggtaactgg acacataacg acgacgacgg tgacgtcgcg ggcgtggggc tggggttgaa
      301 tctgcctctc gggccgttaa tggcgaccgt tggcggaaaa ggcgtgtaca ccaacccgaa
      361 ttacggcgat gaaggttatg ccgcagcggt aggaggtggt ttgcagtgga aaattggcaa
      421 cagcttccgt ttgtttggcg agtattacta ctctccggat tcgctctcca gcggtattca
      481 aagttatgag gaagcgaatg ctggcgcgcg ttacaccatt atgcgtccag tcagtattga
      541 ggcgggttat cgctacctga atctgtcggg taaagacggt aaccgcgaca acgctgtggc
      601 tgacggcctg tatgttgggg ttaacgccag tttctgatcc cttccggcgc ggcattcagt
      661 ttcttactac gccttatctc cctttgcgtt atagtgattc gataacaaaa gcaggagata
      721 accatgttaa aagtggaaat gctatccacc ggggatgaag tgttatacgg gcaaatcgtt
      781 gacactaacg ctgcctggct ggccgatttt ttctttcatc aggggctgcc attatctcgc
      841 cgcaatacgg tgggggataa ccttgatgac ttagtcacca ttcttcgcga acgtagtcag
      901 cacgccgatg tgctgatcgt taacggcggg ctgggaccga ccagcgatga tttaagcgca
      961 ctcgccgctg cgacagcaaa aggtgaaggc ctggtgctgc atgaagcctg gctcaaagag
     1021 atggaacgct atttccacga acgtggacga gtaatggcac cgagcaaccg taaacaagcg
     1081 gagctgcctg ccagtgctga atttatcaat aacccggtag gcaccgcctg tggttttgcc
     1141 gtgcagctta atcgttgcct gatgttcttt actcccggcg taccgtcaga atttaaggtg
     1201 atggtcgagc acgaaatcct gccgcgcctg cgcgagcgtt tttctttacc acagccgccg
     1261 gtttgtctgc gtttaactac ctttggtcgt tcggaaagcg atctggcaca aagcctggac
     1321 actctacaac tgccgccggg cgtaacaatg ggctatcgct cctcaatgcc gatcatcgaa
     1381 ctgaaactca ccggaccggc aagcgagcaa caggcgatgg aaaaactgtg gctggacgtt
     1441 aaacgtgttg ccggacagag cgtgattttc gaaggcaccg aaggactgcc cgcgcagatc
     1501 agtcgcgaat tgcaaaaccg ccagttcagc ctgacgttga gcgagcaatt caccggtggt
     1561 ttattggctt tgcaactttc tcgcgcaggt gctccattgc tggcgtgtga agtgattcct
     1621 tcacaggaag aaaccctggc gcaaactgcg cactggatta cagaacggcg ggccaaccat
     1681 tttgccgggc tggcactggc tgtttcgggt ttcgagaacg agcatctcaa ctttgcgcta
     1741 gccacgccag acggcacttt cgctctgcgt gtgcgtttca gcactacacg ctacagcctg
     1801 gctatccgtc aggaagtgtg cgcaatgatg gcactgaata tgctacgccg ttggttaaac
     1861 ggccaggaca tcgctagtga gcatggctgg attgaggttg ttgagtccat gactttatcc
     1921 gtctgatttc gccactctgt gccggtttac tgcaccggca cattccccct ccggcaaaat
     1981 ttgtccaacc ctccctacgg tgagcgtggt gttggggcca gtgtggcacg ggctgcgcgc
     2041 tggggacgca ttgagaatta catggcgcaa gtcaacgatt cgctttgtct gttagtgcag
     2101 gtggaaagta aaacggcact ggataacctg gacgagatcc tcgacgtcga agggattgat
     2161 ggcgtgttta ttggaccagc ggatctttct gcgtcgttgg gctacccgga taacgccggg
     2221 cacccggaag tgcagcgaat tattgaaacc agcattcggc ggatccgtgc tgcgggtaaa
     2281 gcggctggtt ttctggctgt ggctcctgat atggcgcagc aatgcctggc gtggggagcg
     2341 aactttgtcg ctgttggcgt tgacacgatg ctctacagcg atgccctgga tcaacgactg
     2401 gcgatgttta aatcaggcaa aaatgggcca cgcataaaag gtagttattg atatcaaagg
     2461 cccatgggga tcggctgtgg gcctgtgtta attagtggtt attcgctgcc agatcggctt
     2521 cgcttagctg ggtggccgcg agcacttggt cacgatcaat gccctgttcc agcatgcgca
     2581 aagcaatttt aatggcttgt tcatgcatac cttcctgcaa accttctaat ttaccttgct
     2641 gcaaacctac taatttacct tcttgccagc caatttgatg tccttcctgc cgtagccgtt
     2701 cagcaatagt cattaatctc tccttttgta gtggtgaacg ttcggcaatc tcctgaataa
     2761 aacgggtgaa acgggaggta tcgccgcatt gcagcagata attaaacagt gtttgtagct
     2821 ggctgtcatt agtgaagcct ctaaccaaaa gcgtggtgat cctgtcgacc atgccgatta
     2881 aatcgtggtc gcgaatatgc ttttgaatca gttccagcag agctatacgc cgatgttgca
     2941 tgatctcatc gtcaggtacg atggtgatat ccaccaacgg aaaagcttcg gtgtacaact
     3001 gccgggcaag ttgcggatcg tcaaactcat ccagccagtt aagtgagtac gggtagggcg
     3061 atgtttcgcc atgataaaac agcaatggca ccaccagcgg aactctgtca tagcctttat
     3121 ctaggtgact ctgcatggcg gcagtggcat agcgcattgg ccgaaaagcc atattctttt
     3181 ctgcagagct ttgatgttca atcacgcagt agatatagcc gtcaccgtcg ctggtttcca
     3241 cggaccacaa aacatccgag tagtaagcgc gtaaactttt ttcaataaaa ctagtgggtt
     3301 ccaggcgtaa ggtttgcagg ttgcaaagct tgcgcagtgg ttctggtaaa tgtatttcga
     3361 gaaaatcccg tgcggtttcg ggtgtgaata taaaggtttt aaataccgca tcatgcgggg
     3421 aggaggttgt tgattcggtc atggttgtcc gtcacctgat atttatgatg acgcgacatt
     3481 acggttatcc caggcctttg acatctgcca gtttcagcct ttgcgagacg ttttcagagg
     3541 aattttcact gcgttgcaac gcatacattg ggcacggaat cgtgtacgca aaaatgcccg
     3601 gtaaccacat gcttaccggg catcagaagg gagtttaagc cagcgcctgg gccagtagcg
     3661 taatcggatg ttcgcagcga agacttgtgg acatctcaat ctgccattta caggtttcgc
     3721 agtcggtgac caccagatct gcgccgcttt cttctatctg gcggaacagt ggtgcgccga
     3781 tggcttgtga ggtggggtag ttctcttttt tgaaaccgta agtacccgca ataccgcagc
     3841 actgggaatc cagcaccgtt aactcaagcc ccgggatgtt acgcaacagc tccagggtgt
     3901 agagcgtcca gcccattttt tccatatggc acggagtgtg ataaaccact ttcagcggca
     3961 gcggtttcag cggtaacgtt ttgccttcgt ccagcttgcg ccacagccag cgggttgcca
     4021 gttcgatatg atcgcgcaag cctttgttgt cgacattcag cacttccggg tattcgtcgc
     4081 gcagggcaaa tgtacaggtt gaggaggtgg caatcaccgg aatgcctttt actcccacag
     4141 cttcgcggat cgactctaca ttcgtaattg cctgtttgcg tgctttatcg gtaaagccgt
     4201 tggcgattag cggtacgccg cagcattttt ctttgctgag cagttgtaca ccggtaccca
     4261 ttgcgttgag cactttaatt aaatctttac ctaactgcgg atggttgtag ttaacgaagc
     4321 agccgtgaaa gaaagcgacc tggtctttat attgtgcttg ctgagccgct atgctgcgat
     4381 accagcgacg gaacgtgccg aaggagtatt tcggtagcgt gcgacgatga tcgattttta
     4441 acgccgcatc aagcagctgc cgcaccggtt tcagcgaggt agcggtgttg acgattggtg
     4501 cgaacggcgt ggaaacgcta cccatcaggt cggtatgact caacacaaaa ttacgcagcg
     4561 acgggcgcgt ggtgtcatat ttcgcccgcg cgcgctggat aatatcgcca atcttcacat
     4621 cggacgggca ggcgacttca caacgtttgc agttgatgca atatttcagc gcctcgtcat
     4681 acagtgcgcc atctttcaaa cgcagacgct cgccatccgg cccggcttgt tttggccctg
     4741 gataaccggg attcacccgg ctcaccgggc aggcggtggt gcagacggtg cacttaatgc
     4801 agttttcgaa gctggtgtcg ttcattgttg gcctcctgcg cgttgggcaa tctgttgagc
     4861 ggcatgtaaa gcactgacgg cacaaacacc gccgccgcat ccctgggcga tgggatcaaa
     4921 tccgcccagc accgaaccga tggcaaacag gttctcaatg gtttgccctg cctgcgacgg
     4981 gcgtagcgtc tcatcagtgg ttacaccgaa ctgctgccac ggttgcggcg caaaaaaatc
     5041 gcccttatac cattcacccc gcgtggcggt ttgtagcaca tcaaggccga gaatcggctc
     5101 gcgaatgccg ttacgttccg ctactagtcc gccactaaag aaactgccgc tggcgagaac
     5161 cgcgaaacgt ggacgtagcg gaatatcggc gtgattgcgg gtccagattt cattcactac
     5221 gccattttta caggtcactt ttttcacttc atcgcccggc atccacactc caccttggcg
     5281 cacaaactgg cgctgtaact ggttttgcag acgaatgccc agcacggaag gcggcagcgt
     5341 tggcaaaagc atcagtgaac aaggtagttt ttcattcaac caacgccaca gtttgtcatc
     5401 ggccaggccg aagcaggcgg gcatcaggat catttcgcag gtattggcga caggaataag
     5461 cgcatcaagt aacagcggcc agttttcttc attatcaagg aaacgggcga tattcaccgc
     5521 gcgaaattcg gtggcgttat tgcgcaggac atccagttcc ggcagctcta tttctgcggt
     5581 ttcaaccgaa agatccagtt cgcgcaacga agccgccgca agatgcgcct ggaaatccat
     5641 cagcccgctt atccccacta cacatatctt cttcgcgggt agcggccaga cgggaacttc
     5701 tggcgaactt aaccaggttg cacgcaaagt gccgagcggc gtaatccgct ggtgagccag
     5761 ttctacgctg ccctgcaatt gcgcaccgct ttcagcgatt aatgcctgcg cctggcaagc
     5821 gagatcgagc acgcgttgtg gcccgagaag agagtaagga tgtgcaggtg cctgctgacg
     5881 caaagattcc agtccactgt gaatgtctgc cacgggttga ccatctggca gatggctcag
     5941 caaatccagc gaaccggatg agaaatgcaa cgcgctttga ccacgcgtga caatggcaca
     6001 gcgcaggccg tgtttttgca gttgcaggcc acagagtaat ccggcgaggc cgccgcccat
     6061 aatgacagta tcaaagcgca tctttctgct ccttctccag accacacagt ccctgataaa
     6121 cccagcgggt aaattcgctt tcgcgcagtg cgtctcccca ggcgatgggt tgcacgcctt
     6181 tccagcgttc gttaaggaag gtggaaagtt gctcgataga ttgcgcggac gtcgtgacgt
     6241 taaaccgttg cagcagtccg gcagcgcggc aggcgcagag ttcgccctgg caggtgccca
     6301 tccccacacg ggtacgacgg cgtaaatcca gcaggctatt aacgtttaaa ttttctaccg
     6361 cgtactgcac ttcacccgca gttaccgctt cgcactcaca taccaggcta cggtgtagac
     6421 ggccttcgct cagccaggca ggcgtgcgat cgccatgacg ataaaccgca gaaccgcgca
     6481 gcggggcagg cagggagatg actttacgca aggtaacttc agcgggttct tgtgaaccag
     6541 gcagtgccag atcggcagtc gtacaggggc gcgtgttgcc cagtttgcgg cataccgcgt
     6601 cggtagccca ttcagccatc agccgatagg tcatcagttt gccaccggtg atggtgataa
     6661 atccgtccag accatcgcgt tcagcatggt cgagcagcac gatgccacgg ctgacgttac
     6721 gtccgctcgg gtcgtcatcg ctggcaacca gcgggcgcac gccagaatag gcccgcaaaa
     6781 tgcgcgtttt cgccatcacg ggggccagtt tttccccttc acgcagcaga atatcaacct
     6841 cttctgccgt cactcgatta tcgtcaatct cgttgtaatc aatacgtaaa gaggtggtac
     6901 caatcagcga aatggtatcg ccaggcacca gaatatcggc gtcggaaggt ttacggcagc
     6961 ggttgatcac atgctggtta atgcggtgat ccatgatcag cagcgatcct ttcgccggga
     7021 acatgcgaat gcgcagatcg gcatattcgg caatgtgttg cccccagatc ccagcggcat
     7081 taaccacgac aggtgcatga agggcctgag tttcgccggt gagatggtta cgtacacgaa
     7141 caccgcacac cgtcgcgcct tcacgaatca gccccgtgac ttcatgagcg gtaaggataa
     7201 cggcaccgtg ttctttggca tccagcatgt ttgctgcggt cagacgaaat ggatcaacgg
     7261 tgccatccgg aactttcacc gcgccaatca gtgccgggtt aacggcaggt tcgataatgc
     7321 gcgcttgctg cgggtctata gcttctgcgc tgatccctgc ttcttcgcag gcgcgaataa
     7381 aagtggcctg gaatgagagg tcatcttccg gcagggtgat aaacaggccg ttggttggtt
     7441 caacgcagtg acgtgcaatg cgtttcagga tctggttttc actaatgcat tcgcgggccg
     7501 attccgcatc ggttactgca tagcgcgcac cgctgtgcag caggccgtgg ttacgcccgg
     7561 tggcaccggt tgcgatgtcg tggcgctcaa ccaaaatcac gcgcagcccg cgcagggcac
     7621 agtcgcgggc aatcccggct cccgttgcgc cgccgccaat gataatcacg tcacttgatt
     7681 gcgagtcgcg agttttcatt gttatccctc tgaagttcgt tttttaccat ttagccatag
     7741 taaaaacatg aattgtttga tttcgcgcat attcgctcat gattcgaaag taaaacgtga
     7801 tttcatgcgt cattttgaac attttgtaaa tcttatttaa taatgtgtgc ggcaattcac
     7861 atttaattta tgaatgtttt cttaacatcg cggcaactca agaaacggca ggttctctca
     7921 ctgaatcagg ctgttaatca taaataagac cacgggccac ggaggctatc aatgttgagt
     7981 atttttaaac cagcgccaca caaagcgcgc ttacctgccg cggagatcga tccgacttat
     8041 cgtcgattgc gctggcaaat tttcctgggg atattctttg gctatgcggc ttactatttg
     8101 gttcgtaaga actttgcgct tgctatgcct tatctggttg agcagggatt ctcacgcggt
     8161 gatttaggtt ttgccctttc ggggatctcg attgcttatg gattttcgaa attcatcatg
     8221 ggttcggtat cggatcgctc gaatccgcgc gttttcctgc ccgcaggttt gattctggcg
     8281 gcggcagtga tgttgtttat gggctttgtg ccatgggcga cgtcgagcat tgcggtgatg
     8341 tttgtactgt tgttcctctg cggttggttc caggggatgg ggtggccgcc gtgtggtcgt
     8401 actatggtgc actggtggtc gcagaaagaa cgtggcggca ttgtgtcagt gtggaactgt
     8461 gcgcacaacg tcggtggtgg tattccgccg ctgctgttcc tgctggggat ggcctggttc
     8521 aatgactggc atgcggcgct ctatatgcct gctttctgcg ccattctggt ggcattattc
     8581 gcctttgcga tgatgcgcga taccccgcaa tcctgtggct tgccgccgat cgaagagtac
     8641 aaaaatgatt atccggacga ctataacgaa aaagcggaac aggagctgac cgcgaagcaa
     8701 atcttcatgc agtacgtgct gccgaacaaa ctgctgtggt acatcgccat cgccaacgtg
     8761 ttcgtttatc tgctgcgtta cggcatcctc gactggtcac cgacttatct gaaagaggtt
     8821 aagcatttcg cgctggataa atcctcctgg gcctacttct tctatgagta tgcgggtatt
     8881 ccgggcaccc tgctgtgcgg ctggatgtcg gacaaagtct tccgtggtaa ccgtggggca
     8941 accggcgtct tctttatgac actggtgacc atcgcgacta tcgtttactg gatgaacccg
     9001 gcaggtaacc caaccgtcga tatgatttgt atgattgtta tcggcttcct gatctacggt
     9061 cctgtgatgc tgatcggtct gcatgcgctg gaactggcac cgaaaaaagc ggcaggtacg
     9121 gcagcgggct ttaccgggct gtttggttac ctgggcggtt cggtggcggc gagcgcgatt
     9181 gttggctaca ccgtggactt cttcggctgg gatggcggct ttatggtaat gattggcggc
     9241 agcattctgg cggttatctt gttgattgtt gtgatgattg gcgaaaaacg tcgccatgaa
     9301 caattactgc aaaaacgcaa cggaggctaa tggcatgaaa ttgaagctga aaaaccttag
     9361 catggcgatc atgatgagca ctatagtcat gggaagcagt gcaatggcgg cggacagcaa
     9421 cgaaaaaata gtcatcgccc atcgcggtgc cagtggatat ttgccggagc atacgctgcc
     9481 agcaaaagcg atggcgtatg cgcagggagc ggattatctg gaacaggatt tggtgatgac
     9541 caaagacgac catctggttg ttctgcatga ccattatctc gatcgtgtta ctgatgttgc
     9601 cgatcgtttc ccggatcggg cgcgcaaaga cggtcgttac tacgcgatag atttcacgct
     9661 ggatgaaatt aagtcgctga aatttaccga aggtttcgat attgaaaacg gtaaaaaagt
     9721 acagacttat ccggggcgtt tcccaatggg taagtccgac ttccgggtgc acacctttga
     9781 agaagagatt gaatttgttc aggggttaaa tcactctacc gggaaaaata tcggtatcta
     9841 tccagaaatc aaagcgccgt ggttccatca tcaggaaggg aaggatattg cggcaaaaac
     9901 gctggaagtg ctgaagaaat atggttacac cggtaaagac gataaagttt atttgcaatg
     9961 ttttgatgct gatgagctga agcgtattaa gaatgagctg gaacccaaaa tgggcatgga
    10021 gctcaatctg gtacagctga ttgcctatac cgactggaat gaaacgcagc agaaacagcc
    10081 ggatggaagc tgggttaatt acaactacga ctggatgttt aagccgggag ccatgaaaca
    10141 ggtggcggaa tatgcagatg gtattggtcc ggattaccat atgttgattg aggagacatc
    10201 gcagccgggt aatatcaaac tcactggcat ggtgcaagat gcccagcaga ataaactggt
    10261 agtgcatcct tataccgtgc ggtcagataa actgcctgaa tacactcctg atgtgaatca
    10321 gttatatgat gctctgtata acaaagcggg tgtaaatggg ctgtttactg atttccctga
    10381 taaggcagta aaatttctta ataaagagta atgttactgc aggatggcgt tcatgcgcca
    10441 tcctgtctca tatcaaattt ttccaatcgc cactaattgt tccatgcaca tttgtagtaa
    10501 aaacagtgga ctgccttcta ccggcttttc tttagtcagc aacgaaatct gtcgatagaa
    10561 ggttggttcg tgaggaacgg aacacaattc acctgcaatg cttttcagcg ttaactctgg
    10621 ttgtaaggcg atgcctaatc cctgtcgaat aaaattcatc gctgtatccg actgattaaa
    10681 ttcacatttt atctgctggg cgcactatta gtggtagtgg ctggtggaat ctatgcgctg
    10741 caaatgaagg gcaaaagcgg tgtcacacct gtagcggcaa aagaaattca ttaatacgac
    10801 acgtttcatt aagattttcc tcaggtaacc acctatagtc attgtcgtta ataaaaatga
    10861 caggcgatag ggtatggcag tttcagcaaa gtacgacgaa tttaatcact ggtgggcaac
    10921 agagggcgac tgggttgaag agcctaacta tcgccgtaat ggtatgagcg gggtgcaatg
    10981 cgtcgagcgc aacggcaaaa agctgtatgt aaagcgcatg acgcatcatc tgtttcattc
    11041 agtacgttat ccgttcggcc gaccaacgat tgtccgtgag gttgcggtaa ttaaagaact
    11101 ggaacgggcc ggtgtcatcg tgccgaagat cgtttttggt gaagcggtga aaattgaggg
    11161 tgaatggcgc gcgttgctgg tgactgaaga tatggcgggg ttcatcagca ttgctgactg
    11221 gtatgcccgg catgcagtat cgccttattc tgacgaagta cggcaagcca tgttgaaagc
    11281 agtggcactg gcgtttaaga aaatgcatag cgttaatcgt cagcatggct gttgctatgt
    11341 tcgccatatc tatgtgaaaa cagaaggtaa ggcagaagct ggttttcttg atctggagaa
    11401 aagtcgtcgc cgcttgcgtc gcgataaagc gattaatcat gacttccgcc agctggaaaa
    11461 atatctggag ccgatcccta aagcggactg ggagcaggtg aaagcgtatt actacgccat
    11521 gtaatcgttg taggccagat aatacgcgta agcgtcgtat ctggcgctag tacccgatca
    11581 caactcgatt tcaatatcgc cttttgcccg gcaacaacag ggcaaaattt cccccggctg
    11641 aataaaggct aacggttcgg caatccagtc cacctgacct gcgaccagtc gggtgcgaca
    11701 ggagccgcag taaccttcgc ggcactggta ctcaaccgcc acattgtggg attccagcgc
    11761 cgccagaagg gaagggtgtt catcctggca cagcagttgt gtgccagtga tgcgcagggt
    11821 aacgcgggcc atcagagctg gaagttactc aaatcgtcgg tgtccacttc cgcgtcaatc
    11881 tgcccgacca gataagaact gacttccact tcctgcggag caacctgcac gttatcagac
    11941 accagccagg tgttgatcca cgggatcggg ttggagcgcg tctggaacgg cagatccaaa
    12001 ccgactgcct gcatgcggat attggtgatg tattcaacgt actggcagag aatatcttta
    12061 ttcagaccaa tcatcgaacc gtcacggaac agataatccg cccagtcttt ctcttgctgt
    12121 gccgcctgga caaacaggtc atagcactcc tgcttacatt cttcggcaat ttccgccatc
    12181 tccggatcat ccgcgccgct gcgcagcaga ttcagcatat gctgagtacc ggtcaggtgc
    12241 agggcttcat cgcgggcaat caggcgaata atcttggcgt taccttccat cagttcgcgt
    12301 tctgcaaagg caaaggaaca agcaaagctg acgtagaagc ggatcgcttc cagcgcgtta
    12361 acgctcatca ggcagagata cagttttttc ttcagctcgc gcaggctaac ggttacggtt
    12421 ttaccgttaa cggtgtgggt gccttcgccc agaaggtgcc agtagctggt catttcgatc
    12481 agttcatcgt aatagctgga gatcccttcc gcacgtttct ggatctgctc gttggtgacg
    12541 atatcgtcaa acacaacaga cggatcgtta acgatattac gaatgatatg agtataggaa
    12601 cgggaatgaa tcgtttctga gaacgcccag gtttcgaccc aggtttccag ttccggaata
    12661 gaaataagcg gcaatagcgc cacgttcggg ctacgaccct gaatggaatc cagcagcgtc
    12721 tgatatttca ggttgctgat aaagatgtgt ttttcgtgct ccggcagcgc ctggtaatct
    12781 atacggtcgc gggagacgtc aacttcttcc ggacgccaga agaaagagag ctgcttttcg
    12841 atcagctttt cgaagatgtc atatttttgc tgatcgtagc gagccacgtt gaccggctga
    12901 ccaaagaaca tcggttcttt gagctgatca tttttcgtct gtgaaaaggt ggtatatgcc
    12961 atgagtgtgt cctgttggga gtttaatgcc ggataaggca ttttacgccg catcctgtgc
    13021 ctgatgcgac gctggcgcgt cttatcaggc ctacaaatcc gagccgtagg ccggataagg
    13081 cgttcacgct gcatccggca cccggagcct gatgcgacgc tggcgcgtct tatcaggcct
    13141 acaaaccgag ccgtaggccg gataaggcgt ttacgccgca tccggcatct caatatcaga
    13201 tcttacatgc gccgctttcg cagccatcgt cctggattga cggtaccaga tcgtcctgcg
    13261 cgtcttcagc gccgtcacgg gtgttctgat aatacagcgt tttgaccccg aatttgtagg
    13321 cggtgagcag gtctttcagc aactgctgca tcggcacttt tcctgacggg aaacgtgacg
    13381 gatcgtagtt ggtgttagca gagatcgact gatcgataaa tttctgcatg atacctacca
    13441 gttgcaggta gccatcgtta cccggcattt cccacagcag ctcataggcg tcgtgcaggt
    13501 gctcgtagtc cggcaccacc tggcgcaaaa taccgtcttt cgacgctttg atgctgacgt
    13561 aaccgcgcgg cggttcaata ccgttagtgg cgttagagat ctgcgaagag gtctcggacg
    13621 gcatcagggc agaaagcgtg gagttacgca ggccgtgcgt tttgattgat tcacgcaatg
    13681 cttcccagtc gtaatgcagc ggctcattag cgatggcatc cagatctttc ttataggtat
    13741 cgatcggcag gatccctttc gcgtaagtgg tttcgttaaa ccacgggcac gcgccttgct
    13801 ctttagccag ctcattagac gctttcagca ggtaatactg aatcgcttcg aaggttttat
    13861 gcgtcaggtt gttggcgctg ccgtcggagt agcgtttacc gtgcttcgcc agatagtaag
    13921 cgaagttaat cacaccaata cccagcgtac gacgccccat cgctccacgt ttggcggccg
    13981 ggatcgggta atcctgataa tccagtagcg cgtcaagtgc acgaaccgcc agaatcgcca
    14041 gctcttccag ttcatccagg ctattaattg cgcccaggtt gaaagcagac agcgtacaca
    14101 gcgcgatttc accgttctcg tcgttgacgt cgttcagcgg tttggtcggc agggcaattt
    14161 ctaggcacag gttagactgg cgaactggcg cgatgttcgg atcaaacgga ctatgggtat
    14221 tacagtggtc aacgttctga atatagatac gaccggtaga cgcacgttcc tgcatcatca
    14281 gcgagaacag ctcaacggct ttcacacgct gcttgcggat gctgtcgtct ttctcatatt
    14341 tggtatacag gcgttcaaac tcttcctgat cggcgaagaa cgcgtcgtac agccccggta
    14401 cgtcggacgg gctgaacagg gtgatatctt cacctttcag caggcgggta tacatcagtt
    14461 tgttgatttg taccccgtag tccatatgac gcacgcggtt gccttcaacc ccacggttgt
    14521 ttttcaacac cagcaggctt tccacttcca gatgccacat cgggtagaac agcgttgccg
    14581 caccgccgcg cacaccgccc tgagagcagg atttcaccgc tgtctggaaa tgtttgtaga
    14641 acgggataca gccggtatgg aacgcttcgc caccgcgaat cgggctgccc agcgcacgaa
    14701 tacgtccggc gttgatgccg atcccggcac gctgggaaac atatttcaca atcgcgctgg
    14761 aggtggcgtt gatggaatcc aggctgtcac cgcactcgat cagtacgcag gagctgaact
    14821 gacgagtcgg ggtacgcacg ccggacatga ttggcgtcgg cagcgaaatt ttaaatgtgg
    14881 aaaccgcgtc ataaaaacgc ttcacgtatt gcaggcgcgt ttcacgcggg tagttcgaga
    14941 acaggcacgc ggcaacaaga atataaagga actgggcgct ttcgtagatt tcgccggtca
    15001 cgcggttctg tactagatat ttgccttcca gctgcttaac ggcagcataa gagaaggtca
    15061 tatcacggtc gtgatcgata aacgtgtcca tctgcttgaa ctcttcttcc gtgtagtctt
    15121 ccagcagatg attatcgtat ttgcccatct cgaccatttt caccacgtgg tcgtacagcg
    15181 caggcggctc aaactggccg taggcttttt tacgcaggtg gaagatcgcc aggcgcgcgg
    15241 cgagatactg ataatccggc gcatcacggg agatcaggtc tgcggcagcc ttgataatag
    15301 tttcgtggat gtcagaggtc ttgataccgt cataaaactg aatgtgggag cgcagctcga
    15361 cctgggaaat cgaaacgtta tgcagtcctt ctgccgccca atccagaacg cgatggattt
    15421 tgtcgagatt gatgcgctct gtgctaccgt cgcgctttgt caccagcaga ttctgattca
    15481 tgtatgtcgt acctgttttt ggaattcttt ccgcaatagt ttcatgcaga ctacctgtag
    15541 taagggaagc tgcttagaac actatatata gggggtatgt ttgggattca ctgcaagata
    15601 gtgtgaaaat gaccctcttg caagtgcata actttgtgga taactcagga aggaaaaagt
    15661 ggctttcgcg caccttaggt cagacaaggt gtccgggaaa gtcaatggga agagaaaaat
    15721 ttgttaaaaa taactgttcg gcgatttctt gaacggcaga ataggttgga taaattgata
    15781 tagatggttt attaatcttt ttgctaatga ttgcaaaaat tgatctaagc acgagtcatc
    15841 acataatctg atgtatcagt tgtggtcaat atataagcga atgtaagcga ttgttgatgt
    15901 cgaaatggtt gttttctatt cggttattgt taaatgc
//
LOCUS       DAZPAB010000082        15847 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZPAB010000082 DAZPAB010000000
VERSION     DAZPAB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.082"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..756
                     /locus_tag="ACHUPG_002300"
     CDS             <1..756
                     /locus_tag="ACHUPG_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HFR0861899.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFY
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
     gene            complement(825..1781)
                     /gene="yjiA"
                     /locus_tag="ACHUPG_002301"
     CDS             complement(825..1781)
                     /gene="yjiA"
                     /locus_tag="ACHUPG_002301"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR0861900.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(1792..1995)
                     /locus_tag="ACHUPG_002302"
     CDS             complement(1792..1995)
                     /locus_tag="ACHUPG_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR0861901.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            complement(2045..4195)
                     /gene="btsT"
                     /locus_tag="ACHUPG_002303"
     CDS             complement(2045..4195)
                     /gene="btsT"
                     /locus_tag="ACHUPG_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR0861902.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            complement(4588..5100)
                     /locus_tag="ACHUPG_002304"
     CDS             complement(4588..5100)
                     /locus_tag="ACHUPG_002304"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR0861903.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            complement(5118..6676)
                     /gene="hpaB"
                     /locus_tag="ACHUPG_002305"
                     /pseudo
     CDS             complement(5118..6676)
                     /gene="hpaB"
                     /locus_tag="ACHUPG_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            complement(6903..7840)
                     /locus_tag="ACHUPG_002306"
                     /pseudo
     CDS             complement(6903..7840)
                     /locus_tag="ACHUPG_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            complement(7867..8757)
                     /gene="hpaA"
                     /locus_tag="ACHUPG_002307"
     CDS             complement(7867..8757)
                     /gene="hpaA"
                     /locus_tag="ACHUPG_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR0861904.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            complement(8767..10122)
                     /gene="hpaX"
                     /locus_tag="ACHUPG_002308"
     CDS             complement(8767..10122)
                     /gene="hpaX"
                     /locus_tag="ACHUPG_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR0861905.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCPTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            complement(10144..10947)
                     /gene="hpaI"
                     /locus_tag="ACHUPG_002309"
     CDS             complement(10144..10947)
                     /gene="hpaI"
                     /locus_tag="ACHUPG_002309"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR0861906.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            complement(10958..11761)
                     /gene="hpaH"
                     /locus_tag="ACHUPG_002310"
     CDS             complement(10958..11761)
                     /gene="hpaH"
                     /locus_tag="ACHUPG_002310"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR0861907.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            complement(11829..12209)
                     /locus_tag="ACHUPG_002311"
     CDS             complement(11829..12209)
                     /locus_tag="ACHUPG_002311"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR0861908.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            complement(12219..13070)
                     /gene="hpaD"
                     /locus_tag="ACHUPG_002312"
     CDS             complement(12219..13070)
                     /gene="hpaD"
                     /locus_tag="ACHUPG_002312"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR0861909.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            complement(13072..14538)
                     /gene="hpaE"
                     /locus_tag="ACHUPG_002313"
     CDS             complement(13072..14538)
                     /gene="hpaE"
                     /locus_tag="ACHUPG_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR0861910.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            complement(14535..15824)
                     /gene="hpaG"
                     /locus_tag="ACHUPG_002314"
     CDS             complement(14535..15824)
                     /gene="hpaG"
                     /locus_tag="ACHUPG_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR0861911.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
BASE COUNT         3728 a         4141 c         4155 g         3823 t
ORIGIN      
        1 tatactaaga ttttagagaa tgattttgaa cacccttcaa tacgacgaaa aatagcatcg
       61 ctaagtaatt taggggtaga tgagtggatt cctccggtta tggcattcat gaatcgaatg
      121 gctcgtacag aagattttaa tttagatgat ttttctcaat ttattaccgc ctttgagaag
      181 gtatatatgc atgggtggtt aaaaaaacaa attaaaagcc aaagagaaat ggtttgttat
      241 tctgctctgg tagcaataaa taatgatatg ccttttgatt ctgtgataaa tcagataaac
      301 caacatgcag ataattcagg ttttatagct gctctggatg aagatttata tgagcctcgt
      361 ccgaatcaag tgaacttaat aaaagcaatt ttgcttcgct tagatatgga gcaacaagat
      421 gagagcgtga ttaaaaccta tacggggaga atcacaattg agcatattct tcctcaagca
      481 ttagttaatg aatactggat taatagattc caacctcaag agcatgtcta ctggctgcat
      541 aaaataggta atttaacact cataagtggt tccaaaaatt ctgaaacaca acactatgat
      601 tttatcaaaa agaaatctat ttatgagaaa cttaatagta aaagctcttt ttatttaaca
      661 aaagatgttt gtaattcttc agaatgggga ttggctgaac tgaaaatgag acatgaaaaa
      721 atgaaaacgc aattgaagaa gctttggttg gtctgacata tctaaagagt tccactcgta
      781 ataatgttgc ggtggaactc tcaaaaagtt gatctttccc gaacttactt cctcaatccc
      841 gcaaacgccg cccgaatctc ttcctccggc aactgaatcc cgataaacac cattgtgcta
      901 tgcggttttt catcgcccca cggcctgtcc cagtcggcgc tgtagaggcg ctggacgccc
      961 tggaacagca ggcggttagg ttcgccgtca atccacagca tccctttgta acgcagcagt
     1021 ttatccgccg attccagcag caggttttcc atcacgcggg aaacttcgct gatatctacc
     1081 gggtaatcca gttccaccac aattgacgaa atatcgtttt gtttatccgc gataaagtgg
     1141 aaacgcggtt tggtgctgac gacgttctct tccagcataa aaccgttggt gttgaacagc
     1201 aggcccaggt cgatgtcgcc gtgggtgacg gtgtagaccg gtgcgcgggc gttgatgcgc
     1261 gccaggcgtt cacgcagttt ctctgcttcg cctgcgacgt cggttttagt cagcagaata
     1321 cggtcggcgt agcccacctg ggactgggcg atggtgaact ggttcatctg ctcatcggca
     1381 tgtaccgcat ccaccagcgc aatcacgccg tccagcaggt agagctggca taaaatctca
     1441 tgggagaaaa aggtctgaat aatcgggccg ggatcggcca tgccggtgca ttcaatcacc
     1501 agacgatcga actgaatatt gcccttgtcg agattatcca gcaggtcgag tagtgcgtcc
     1561 tccagctcgt tggagcgcga acagcagatg cagccgttgg tcagcgtttt gatctgcgtg
     1621 gcgcggtcgc caatcagttg gtcatcaacg gagacttcgc cgaattcgtt ttcaatcacg
     1681 gcaatcttgt agccgtgttg ttcgttaaga atatggcgca gcagggtagt tttgcccgcg
     1741 cctaaaaaac cggtgagtag ggtaactgca atcgggttca tgctctctcc attaacaaca
     1801 gcgcataccg cctttaccat cgccgccgta gcgcgcgttc tggcgttcgc ggaagaattc
     1861 ttcatagctc atgtacggct tgtcgggatg gttggtcttc atatgctcga cgtagttgtc
     1921 gtagtctgga ataccaatca acatcttcgc cgcctggccg agatattttt ttgcctgtcc
     1981 taagttacca aacatagttc actctgataa gaacaaagcc ccgccgaagc ggggctaaac
     2041 acggttagtg gtgcgaagag atcttcacgc cgccttccgg gattggaacg tacggtgttt
     2101 ctttgtcagt acgtttgtcg ctgttacgca ccgcaagcca ggttttgaaa ccgtagaaga
     2161 tgatgctgta caccacaatc aggaacagaa tactcaggcc tgcgttggtg tagttgttca
     2221 caacgatgtg gttcatgttg gcaatctgct gcgccgtcag gtcagtaccg ttagcaatct
     2281 tctctttgta ctggcttgcc atgtagaaga agccttccat ctgcgggttg gtgctgaaca
     2341 gtttcagacc cagcgcccag gtggtgcaga taagcagcca tacagccgga acaacagtta
     2401 cccagatgta ttgggtgcgc ttcatcttaa tcagcacaac ggtgcccagt accagcgcta
     2461 cggctgccag catctggttg gagataccga acagcggcca caggctctta acgccgccca
     2521 gcggatcgac cacgccctga tacagcaggt agccccacag acccacacag cccgcagtac
     2581 cgatgatacc ggcaaccaga gagtcggttt ttttcaggaa cgggatgaag ttacccagca
     2641 ggtcttgcag cataaagcgg ccagaacggg tacccgcatc cagcgcggtc aggatgaaca
     2701 gggcttcgaa cagaataccg aagtgatacc agaagcccat gtcagccatc ggcagcactt
     2761 tgtggaacac gtgagcgata cctaccgcca gcgtcggcgc accacctgca cggttcagga
     2821 cagaaggctc accaatgtct ttcgcggttt gcaggatctg ctctggcgaa atcacgaagc
     2881 cccaggagct gacggtcgct gccgcgtgtg cggtaacgtc tttcagctgc gccatgatga
     2941 tcggcgcgtt ctcgccaccc atttcatgca ggttaggcat ggtgatgcca aggccagcag
     3001 gcggggtgtt catcgcgaag taaagacccg gttcgatgat ggacgctgca accagcgcca
     3061 taatcgccac gaaggactcc atcagcattg cgccgtagcc aatgaaacgc gcgtcggttt
     3121 cgttagccag cagtttcggc gtcgtaccgg aagagatcag cgcgtggaag ccagataccg
     3181 caccacaggc gatggtgatg aacaggaacg ggaacagagc gcctttccac agcgggccag
     3241 taccgtcaat gtactgggtc atcgcaggca ttttcagttc cgggttcagc accacgatac
     3301 ccagcgccag gccgacgata acgccgattt tcaggaaggt tgccagatag tcgcgcggtg
     3361 cgaggatcag ccacaccggc agcagtgcgg aaacaaacgc atagccaatc agcgcgaagg
     3421 taatggtggt gtctttaaag gtcagtgccg gaccccagta cggatcgtga gcaatcacgc
     3481 caccgaagta gatagaggca accagcagca cgataccaat aacagagact tcacccacac
     3541 gccccggacg gataaagcgc atgtagatac ccataaacag cgcaatcggt acggttgagc
     3601 aaacggtgaa gacaccccac ggactttcgg ccagggcttt aaccacaatc agcgccagaa
     3661 cggcgaggat gatgatcatg attaagaaac agccaaacag cgcgatagtc cccggtactg
     3721 gccccatctc ttctttgatc atctcaccga gagatgcgcc gttacggcga gaggagataa
     3781 acagcaccat aaagtcctgt accgcaccgg ccagcactac ccccgccagc agccacagcg
     3841 tgccaggcag gtagcccatc tgcgcggcga gaaccggtcc caccagcgga ccagcaccgg
     3901 cgatagcggc gaagtggtga ccaaacaaca cgtaacggtt ggtcggaacg tagttcagac
     3961 cgtcgttgtt aataaccgca ggcgtcgcgc gcgtggggtc gagtttcatc accttctggg
     4021 cgatgtacag actgtagtag cgatacgcca ccagatacac cgacacagag gcgaccacga
     4081 tccacagggc gctgacgtgc tccccccgac gtaatgcaac taccgcgagg cagaatgcac
     4141 cgatgattcc gagaatcacc cagggtatgt gcttgaatag ttttttcgta tccatagtaa
     4201 aacctggcat tttactcgtc gtcttttggc ttacaggtgc ggcagctgcg ttcgttcacc
     4261 tctgacactt acttttgtaa gctcccgggg attcactcac ttgctgcctt cctgcaaacc
     4321 aaaatccata gagtaatttg ttaatcatag gccgaagccg ttctaggttt gcgttgcgtt
     4381 tgaggaggta aattgaccgc tatcctgcag tgatgttgcc agagatacgt gcgcgtaaag
     4441 taaggtaaat aactgagtgg ttattttagg gatgtaagcg gtcaggaatg gcggtgagcg
     4501 gttgggcaag aattattcat cgggtagata tgtgattgag atcacaataa aaatgcccct
     4561 tcgacgaatg cgtgaagggg ctggagctta aatcgcagct tccatttcca gcatcaccgg
     4621 atggaaacgg cgtttaaagt aaataagtcc gtgaccttct gcactgagga tgatgttttt
     4681 aatctccacc agatacacca gatgtgtgcc aatcgcctgc acatcgcgga tctctccttc
     4741 aagactggcc agcgaacctt ttagcaccgg ctgcgccagc ggaccttttt gccagcatga
     4801 gaggctaaaa cgctcttcca tcgccatgcc tgtcatgccc gcgaagtggc gcgccatcag
     4861 ttcctgctca tggttgagga cgttgacgca caacttgccg ttgccctgaa aaaccgggtt
     4921 catcgcactg ttggcgttaa tgcacaccat cagcgacggt ggtgtatccg tgaccgagca
     4981 gacagccgtt gccgtaatcc cgcattgtcc ggcgtcgccc tcggtggtga taatatttac
     5041 cgctgccgac aggctggcca tcgcgtcatg aaagcgcagg cgttgttcat ctaattgcat
     5101 cttaacctcc tgctgcgtta tttcagcagc ttatccagca tgttgatatc gtcgttgttg
     5161 tgcaggtgcg gcacagtcca gccgttctgg tcgtattccg acaggcagcg atcaaccatc
     5221 gccatcatct tgtccatatt gccggagctt tgtgcctggc gcagacattg caggcgaatc
     5281 tcatcctggc taccggagta gttgatttca tacagctcgt gacgaccacc aaattcgctg
     5341 ccaatggcgt cccacatcag tttgaggatc ttgatgcgct ggacgtgatc cataccgttc
     5401 gaaccgcgca catacttcgc cagatactgg tcgatctgcg gattgttcag gtcacgggca
     5461 ctggaaggga ggtagatcag gccactggta acgttgcgtt ctataatgtt tttgatcttc
     5521 gcgtaggcca ttggtgccag tacgcgatag gtttgcagtg cggcatgatc cggtaaataa
     5581 gccccgttga cccacggcgt cgcttctgaa cacatcgagt cactcaatgc ccagaaggtg
     5641 ttgcgccacg ccaccacttc accgagatcg gcctgcacac cacggaactc cagggtgccg
     5701 gtacattcga gtgatttttt cagcagtgcc gtaatgaagt cgagtttcac tgccaggcgc
     5761 acacaggctt gcagcggata catacgggcg aaaccgcctt ccatcgtcca gcgacggcag
     5821 cgatcaaaat cgcggtagat cagcacgttt tcccatggga tcagtacgtt atccatcacc
     5881 agaatcgcat cgttctcatc gaagcggctg gagagcgggt agtcgtatgg cgagccggta
     5941 gcacccgcga ccatctcata agaggcgcgg gagattaatt tcacgccatc ggcatccatt
     6001 ggcgcaacga acatcagtgc gaagtccggg ttttcgccca tcacttgtgc cgagccgaag
     6061 ccaatcatgt tgtagtgagt cagcgccgag ttggtggcaa ccactttcgc accgctgacg
     6121 ataatcccgg cgtcagtctc tttttccagc ttgatgtaaa cgtctttcac tttatcggtc
     6181 ggcaaatgac gatcggtggg ttaacaatcg cgtggttaaa gtagaggcca gtttcctgaa
     6241 tacgggtata ccagttacgg gcgttctgct cgaactgacc gtaaaagccc ggattcgcgc
     6301 ccagtgcgca accgaaagcg gctttgtagt ctggggtacg gcccatccag ccatagctca
     6361 ggcgtgacca ttcagcgatg gcgtcgcgtt gctggcgcag gtcgtcggca cttttcgcca
     6421 cgcggaagaa tttatgggta tagccgccgc tgccggtgtc ggtgttccaa cacagagagt
     6481 cctgcatctc cggtttgtgc agcgcgtcgt acagttgggc aacagacgct gccgcattac
     6541 gaaatgccgg atgagtggtg acgtctttca ctcgctcgcc atagatatag atctcgcgac
     6601 catcctgcag gcttttcaga tactcttccc cggtgaaagg acgttgggta ctggcgcgga
     6661 aatcttctgg tttcatgtcg acctctacag tattaatcgg aatacgattg ttaattttgt
     6721 gtgttgtttt tgttatttaa ttgaagcgtt gaacgcggtt gtcgggaaga gacgtttcag
     6781 gcgaacgttg gtacttttcg acagcaaggt ggagttaaat agtaaactgc gatcgtgatc
     6841 aacttacagt aaggcaaatc ataatattcc ccgctaacaa gggctaacgg ggacggaatt
     6901 ttttatgggg cgaatggaat ggtaatcgtc atattttcat gaccaacgac gataggacct
     6961 gaatagtttt ctgtgcttct tgcttccatg tttcatccgg aacggtgatt ggtaccaggt
     7021 ggctaaggat gagttttttc acttttgctt cattggcaat tttgcctaca tcctcaatag
     7081 tcgtatggtg agaagcaatg gcttttgcca gtgttttacc attgccaata ctgtctgcca
     7141 gcttagcgac ggagggctca tgtacggcct catgcacaag tatatcagcg ccttctgaga
     7201 aagttgccag tgggggaaag tacgaggtat ctcctgagaa gacaattcgc ttgccttgtg
     7261 tgtcgaagcg ataagcaaaa gcctcaccgt cagggaaagg agaatgaggg acctttaatg
     7321 cagtcacggt cacggtatca tctttataaa ctacgccttc accaacatct tgagctgtaa
     7381 cgagtttgcg aggatcgggt tttccttctt cttccatacg cagtgtgatg tcccgatcga
     7441 agacctgcca actggcagcg gtcatggctc gggtgcctgg cggaccgaat acatgaatag
     7501 ttttgtcctg cagaccgcta gcccatgcat tcattaagac tgatggatag tccagaatat
     7561 gatcactgtg taaatgggtg agtaaaatat tatcaatctg gcgcagtgga atattatttt
     7621 ttaccagttg taagctggcc ccgtaaccag catcaattaa ccaggtttta ttgccaataa
     7681 taagtgccgt tgccatcggt aaacggtcag cttttaataa agaagggcca cctttcgttc
     7741 caagtagtac aattttcatt tattgcggtt cggaggcgta ggtgaatgcc actggcgcaa
     7801 tcaggcacgc cacaaatagt gagcgtaaag atattttcat gtcgagtctc tcaggttgat
     7861 aaatggtcag cgaccacgga attgtgttgg tgagcaaccc atcattcggt tgaaaaaacg
     7921 ggcgaaatag gcgggatctt tgtagccaag atcaagggct atctggttga tgctttgctc
     7981 gctatagagc agaagccgtc tggcttcccg gctcactcgt tcgaaaatca atctcttcgg
     8041 cgattgattg gcaaaacgtc ggcacagctc ggtaagccgt gattcactca taccgagcat
     8101 tgttgcatat tcagggacca ttaaatgttg gcgataattt tcgtcaatca gccgattaaa
     8161 cttctggaat aaacacatct cgccgcgcac gctgcatgtt gaaatatcat ttggtggtgc
     8221 ttcacgcagt aggattgtaa aaatactttg cgccagtgat tgcaatagaa tttcccgtcc
     8281 ttgcttattt tgttgaaact ccagggatat tatcggccaa taatgattta atgccgccag
     8341 tgtatgttcc attccctcca gtgacagaca gataccttgt agcattgcgg catctccact
     8401 accaggccag agcttttcta ccaggggcca aattaactcc tgacgtacgg tcagaacatg
     8461 cccatcgcta tcaggatcag taaagaaagt atgtggcacc gatggtggtg ttagtacaaa
     8521 aagcggtgcg cgcacagaat agaagtgctc atccagttgt aaagtaatgc gtccagtgac
     8581 cagataatgg agttgaaaat atctatcgtg ccagtgaggg cgcatatcgc gtccaaagaa
     8641 attcgctaac cgggcaaatg tttcataatg cacatcttca ccagcgtagc gggcatcata
     8701 ttcccggacc aaatcaatat tttcataacc atgttcaaag atagcttcac tggacatcat
     8761 gactccttat tgcggttttg gtgcgataag ttcagatgaa agagagtgtt cccgactccc
     8821 catagggatt cgggtcatga ctaacgcgcc cagtaccagc aatcctgcta caaaccacaa
     8881 acctgagcta aaactgccgg ttgcatcttt caaaataccg ataagtaatg gactgactgc
     8941 tgaacctaca ttgccgacag cattgatgac agccatcgca acggcacgcg attgcaagct
     9001 tataacttga tccggtgttg tccaaaagat agtcatcgcg gtaaatgatc ccattgaggc
     9061 cataatgatg ctgctcagtt gaatgaggct gtgatctgtc gcagaagcga gtaaccatcc
     9121 ggcagcggca aacaggtagg gaagaatcgt atgctttttg cgttctttaa gacggtcgga
     9181 acgacgactc caccagatca tgccaaaaat tgtgcagaat tgggggatgg ccgccagcaa
     9241 accaatcatg atgttgctac tgccagtatt gaaactctgt aggatttgcg gcgtccagat
     9301 attaatcgca ctcagagtat tagtcgggca aaaataagca agtgtatata gcatgatggc
     9361 tggggtcagg acttcacgta aggttgagcg tggaacagat gcatgggcaa atgcactctc
     9421 ttgttcacgg agaatcatcg tttttaaagc ctgtttttcg ttttcatcaa gccaggtcgc
     9481 ctggtctggt gtgtcgttga gataaaacca ggtgacgaca ccaagaatga ccgatggcag
     9541 accttccagc aaaaagagcc attgccagcc ttttagattc cacagtccat ccaaggccag
     9601 aatgtagcct gaaaggatca atcccagcat catagtgact ggcatcgcta tcataaataa
     9661 ggcattggct ctggcccgat gatatgccgg gaaccaccag gtcagataaa ccaaaatacc
     9721 aggtaaaaaa cccgcctcag caataccaac cagcatacgc agtagataga gtgtttcggg
     9781 gcttgttgca aacattgtgc aggtggaagc aattccccag accaccatga tcccagcgat
     9841 ccatcgacga gcgccaattt tcgctaacat catattactg gggatcccgc acatgacgta
     9901 cgtgacataa aagagtgttg cggcgaggcc aaacattgtg gacgtcaggc caagatcctt
     9961 gcccattgtc agtccggcaa atcctatatt gatgcgatca agaaacgaaa aaacaaacag
    10021 aataaaaaga aaaatgatta ggcgacgaaa taatttatta atgacacgat gttcaatttc
    10081 aacagtttta ttatctttat attgcagggt agaggtcgtc atgatgcgct ccacatcgta
    10141 cgtttagtag acagagttgt tgttttgctt cgccatcgaa atagaagaac tattaccaaa
    10201 ccgtgctgcc agcgcttcgg cggcgcgggc gagcagggtg gtgtcaacgc cgacggcgac
    10261 aaacagcgcg cccagttcca gatagcgttt tgccagttgc tcattggcga tcaggatccc
    10321 cggcgctttg cccgcttcgc ggatctgcac gatcgcctgc tcaatggcgg cctgtacttc
    10381 cgggtgctgc ggattaccgg cataacccat atcggcgctc agatccgccg ggccgataaa
    10441 cacgccgtcg accccttcca cgtccagaat ctgcggtaag ttcttcattg cctcacgcgt
    10501 ttcgatctgc accagcacgc acatttgatc gttggctttt tgcaggtaat caggaatgcg
    10561 attccagcgc gaggcgcgag ccagcgcact gcccacaccg cgaataccgg cggggggata
    10621 acgggtggcg cgtaccgctt tacgggcttc gtcggcgttt tgtaccatcg gcaccagtaa
    10681 ggtttgtgtg cctacgtcca gcagttgttt gatttgcacc ggatcgttcc acgatgggcg
    10741 taccactggc tggctggggt agggcgcaat cgcctgtagt tgggtgagca cggtttgcac
    10801 gttattcggc gcgtgctcac cgtcgatcaa taaccagtcg aatcctggtc cggccagtaa
    10861 ctccgcgctg tagctgctgc tcagccccag ccataatcca atctgcggac ggcctgcttt
    10921 cagcgccgct ttaaaactgt tttccatcgt tctctcctta aacaaagcgg cagctaatgg
    10981 agcccatgtt gccgtaatcg acgtggaagg tgtcgccctt acgcgccgga accgggaggg
    11041 tgaacgaacc accgagaatg atttgcccgg tttccagttg tacgtcatac ggtgcgagtt
    11101 tgttcgccag ccaggccacg ccgtttgccg gatgattcag cacaccagcg gcgacgccgg
    11161 tttcttcaat cacgccattg cgatacatca gggcggagat ccaacgcaga tccagttcgt
    11221 ctggtttgat cggacgacct ccgaggatca ctccggcgtt ggcggcgtta tcggaaatgg
    11281 tgtcgaatac tttgcgcggg cgctgggttt ccggatcgat gttgtggcag cgagcgtcga
    11341 tcagctccag cgccgggatc acatagtccg tggcgttgta aacgtcgaac agcgtgcagt
    11401 ttggcccacg cagcggtttt gccagcacaa aagccagctc cacttcaatg cgcggcacga
    11461 taaagcgatc ggtcgggata tcgctgccat cgtggaagaa catgtcgtcc agcagtgcac
    11521 cgtaatccgg ttcgctgatc tgcgagcttg cctgcatcgc tttcgaggtc agaccgattt
    11581 tgtggccttt cagcgtgcga ctttcggcga ttttcaggcg aacccattcg cgctgcacgg
    11641 cgtaggcgtc ttcgatggtg atctccgggt aatccagcga gatcgcgcgg atctgttcgc
    11701 gctgtttttc tgcctgatcc agacgctggg cgatcagggt gtgggtgtgt ttatcgaaca
    11761 tagcgatatc ctgtggcatt gccggatgcg gcagcgcctt atcatccggc ctggaagctg
    11821 gtgcaaattt atttaaacaa cgcgtgtacg ttgttctgtt tgaaattcaa cgtcgggtgc
    11881 agctctgcaa tctcaaatga aagcgctagg taacggctct ccatcagtga ggcgaagtgc
    11941 gttttaatca gatcaaacag catttcgccc acttcctgac ggctttccag actgcgtccg
    12001 ctgccgattt ttagcgtcat atgcacaaag gcgtaatcgt gcttgccatc agccatttgc
    12061 caggtatcga tccagtgcgc ccggctacgt attcccccaa gcggaaaaat ccccgttgcc
    12121 gccagtgcag tgttaacact ggcaaacagc tcaggtaagc gggcttcttc acgaatattt
    12181 tcagtacatt ccacgataaa gtgcggcata aaacgtcctt acgcgggcag cgggaaaaca
    12241 gcgttaacct gaccggtgcc agagcttggg aacagctcgg taataaactc caccttgccg
    12301 tcgtatttat cccagccgag catccccagc agcatcaccg tgtcgtgcat attgccttcg
    12361 ccgtagcagt agtcggcgta ctccggcagc atattgcaaa actctttgaa ctggccttcg
    12421 cgccacagct tcaccacacg ctcgtccatc tggcggtcga actcgcgggt gtagctgttc
    12481 atcccttctt ccgcacgctg atcgtcaata aagcggtgcg ataacgaacc gctggcaagg
    12541 accgccacgg tgccgtcgta ctgttcgatc gctttcagaa tcgcttcgcc cagcttgcgg
    12601 ctgtcggcaa aatcgtgaac cgtgcagaaa gccgaaatgg agaccacttt gaagtgcttg
    12661 tcttcattca tgtagcgcat cggtaccagc gtgccgtatt ccagtttcag gctgggaata
    12721 ttgtgcgctt ttgcccgcac gccgagcttt aaggcttcct cggcaattag ctgccccaac
    12781 tccgggttgc cctcgtagtt gtaggtcatg tcgcgaataa aatgcggcag ctcgttgctg
    12841 gtgtagacgc cttcaaaatg gtctgcacag ttgatgtgat aagcactgtt gaccagccag
    12901 tgagtatcga aaacgataat ggtatcgacg cccatttccc ggcaacgctt gctgatctct
    12961 ttatgcccgt cgatcgcacc ctggcgacaa ccgtggtttt ttcctggtag ttcagagaga
    13021 tacatcgacg gtacgtgcgt gatcttggcg gctaacgcta acttacccat atcagactcc
    13081 ccatttcgga attggatggt cgcccatgga aatgcagacg ttcttcattt ccgcgaacac
    13141 ttcgaagctg tactcaccgc cttcacgccc ggtgccggag gcttttacgc cgccaaatgg
    13201 ctggcgcagg tcgcgcacgt tctgggtgtt gacgaacacc atgcctgctt caatgccacg
    13261 cgccaggcgt aacactttgc tgacatcctg tgtccagatg tacgacgcga ggccgtactc
    13321 cacgtcgttt gccaggcgta agccttcggc ttcgtcttta aacggcagca ggcaggcgac
    13381 cggcccgaaa atctcttcct gggcaacgcg catacggtta tctacgtccg ccagcaccgt
    13441 tgggcgcagg aagttgccgc ctttcaggtg cgcaggcagg tcggacggtt tatccgggcc
    13501 gcccgccaac agggttgcgc cttcttcaat gccgagacgg atatagccgg agactttttc
    13561 ccagtgttgc tgactgataa gcgccctaac ctgggtattc ggatcgttcg gatcgcccac
    13621 gcgcagacgg ttggcgcgtt cggcaaagcg tttaacgaat tctggataga tgctctgctg
    13681 aataaagatg cgcgaaccgg cggtgcagcg ttcgccgttg attgagaaga tggtgaacag
    13741 ggcggcgtcc agcgcacgct caatatcggc atcttcaaaa atcagcaccg gtgatttacc
    13801 gcccagctcc atggagtatt ttttcagccc ggcgtttttc atgatgttgc gcccggtggc
    13861 ggtaccgccg gtgaacgaca cggcacgcac gtcatgatga cggaccagcg catcgcctgc
    13921 ggttgcgccg tagccctgta ccacgtttag cacgcccgcc ggaataccag cttccagcgc
    13981 cagctcaccc aggcggtcgg cggtcagcgg ggagagttcc gacatcttca gcaccgcggt
    14041 attgcccagc gccagacacg gcgcgacctt ccaggtggcg gtcataaacg gcacgttcca
    14101 cggcgacgcc agcgcacaaa cgcctaccgg ttgcaccagc gtgtagttga gcatcttgtc
    14161 gtcaaccgga taggtcttgc cgttcatctg ctggcagact tccgcgaaaa attcaaagtt
    14221 gtgagaagcg cgtgggatca acacattttt ggtctgatgg atcggcaggc ccgtgtccgc
    14281 ggtttccatc gcggcgatct caggcacgtt ctggtcgatc agatcgccca gacggcgcat
    14341 caggcgcgca cgctctttca tcggcagatc agcccatttc gggaacgcct ctttcgctgc
    14401 cgctaccgcc tgattgatct ccgcttcacc gccagaggcc acatccgcca gcacttcacc
    14461 cgttgccgga ttggtagtct ggaagtagtc gttacctgca acatttttgc cgttgatcca
    14521 atgatttact tttttcattt cgctgtttcc tcactcacaa ttcggttcac caggcggccc
    14581 acgccttcta cttccaccac tacttcatcg ccaggcacca cgtcagataa gccttttggt
    14641 gtgccggtgg cgatcatgtc gcccggattc agggtcataa attcgcttaa gtaggcgatc
    14701 aggaagggca cgctgaagat cagatcggcg gtggtgcctt gctggcgtaa ctcgccgttg
    14761 acgaaggtgc gaagggtcag attatgcggg tccgggatcg cctctttcgg cacgatggtt
    14821 gaaagcatcg gcgtcagtcc gtcgcggctt ttgacccgca ggttagggcg gtagtagttt
    14881 tccagatagt cgcgaatggc gtagtcgtta cacacggtgt agcccgcgac ataatccatg
    14941 gcatcggctt cgctgacgtt acgcgcctgc ttgccaataa ctaccaccag ctccgcttcg
    15001 tagtgcatgt attcaatatt gttcgggcgc acggaggtct ggttgtcgcc agtgagggta
    15061 ttcggcgctt tcaggaacac cagcggctct tccggtggct taaattccag ttcgctggcg
    15121 tggtcggcgt agttcaggcc gagggcaaac agcgtaccgt gcgggtgtgg cagcgttggg
    15181 aagctcttgc gcgtggtcac ttcacgttcg tccactaccg gattttccag cggcgggaaa
    15241 ccttctgcga gaacacgcac gcgatcgctt ggctgtattt ccacgcgcgc ctgtggcgtg
    15301 ccgagcagaa tagcatcgcc tggattcagc gtggcaaatt cgctcagggc gctcagcagc
    15361 tgtgcggcgt tacgttgtaa atcggcggta ttccagtgat cggcaggacg cccgttgatc
    15421 tcggtataga tagtcagatt atcgacattg ctgagagcca cggtttcgcc aatggggcag
    15481 aatccatcac gacattttgc tttgattgcc ggacggtaaa agctctcttc cggcaggctg
    15541 acgtcgttat ccagcgcata tccggcgatg tactctgccg catcttcttc acgtactttc
    15601 gtcgccgttt tccccacaat cagcgcaaca gtcgcaccgc tcagtacttt ttcaccctgt
    15661 ggaaagggaa tcggttcacc gcaaccaatc accgtattgc gcggtttaat aaaccagacc
    15721 gcagttttag gcggggcttt gtagggagat tgctggaacg cttcctgcca ggcatcaagt
    15781 tggctgcgat ggttcaacgc tacggcgaag atagtgcctt tcattaccac tcctcggatt
    15841 cgatcat
//
LOCUS       DAZPAB010000083        15708 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZPAB010000083 DAZPAB010000000
VERSION     DAZPAB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.083"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..200
                     /gene="tuf"
                     /locus_tag="ACHUPG_002315"
     CDS             <1..200
                     /gene="tuf"
                     /locus_tag="ACHUPG_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFR0861912.1"
                     /translation="QFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGL
                     RFAIREGGRTVGAGVVAKVLG"
     gene            383..577
                     /gene="bfd"
                     /locus_tag="ACHUPG_002316"
     CDS             383..577
                     /gene="bfd"
                     /locus_tag="ACHUPG_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFR0861913.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            650..1126
                     /gene="bfr"
                     /locus_tag="ACHUPG_002317"
     CDS             650..1126
                     /gene="bfr"
                     /locus_tag="ACHUPG_002317"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFR0861914.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1123..1590)
                     /locus_tag="ACHUPG_002318"
                     /pseudo
     CDS             complement(1123..1590)
                     /locus_tag="ACHUPG_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
     gene            1969..2280
                     /gene="rpsJ"
                     /locus_tag="ACHUPG_002319"
     CDS             1969..2280
                     /gene="rpsJ"
                     /locus_tag="ACHUPG_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR0861915.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2313..2942
                     /gene="rplC"
                     /locus_tag="ACHUPG_002320"
     CDS             2313..2942
                     /gene="rplC"
                     /locus_tag="ACHUPG_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR0861916.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2953..3558
                     /gene="rplD"
                     /locus_tag="ACHUPG_002321"
     CDS             2953..3558
                     /gene="rplD"
                     /locus_tag="ACHUPG_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR0861917.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3555..3857
                     /gene="rplW"
                     /locus_tag="ACHUPG_002322"
     CDS             3555..3857
                     /gene="rplW"
                     /locus_tag="ACHUPG_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR0861918.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3875..4696
                     /gene="rplB"
                     /locus_tag="ACHUPG_002323"
     CDS             3875..4696
                     /gene="rplB"
                     /locus_tag="ACHUPG_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR0861919.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4713..4991
                     /gene="rpsS"
                     /locus_tag="ACHUPG_002324"
     CDS             4713..4991
                     /gene="rpsS"
                     /locus_tag="ACHUPG_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR0861920.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5006..5338
                     /gene="rplV"
                     /locus_tag="ACHUPG_002325"
     CDS             5006..5338
                     /gene="rplV"
                     /locus_tag="ACHUPG_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR0861921.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5356..6057
                     /gene="rpsC"
                     /locus_tag="ACHUPG_002326"
     CDS             5356..6057
                     /gene="rpsC"
                     /locus_tag="ACHUPG_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR0861922.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6070..6480
                     /gene="rplP"
                     /locus_tag="ACHUPG_002327"
     CDS             6070..6480
                     /gene="rplP"
                     /locus_tag="ACHUPG_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR0861923.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6480..6671
                     /gene="rpmC"
                     /locus_tag="ACHUPG_002328"
     CDS             6480..6671
                     /gene="rpmC"
                     /locus_tag="ACHUPG_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR0861924.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6671..6925
                     /gene="rpsQ"
                     /locus_tag="ACHUPG_002329"
     CDS             6671..6925
                     /gene="rpsQ"
                     /locus_tag="ACHUPG_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR0861925.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7090..7461
                     /gene="rplN"
                     /locus_tag="ACHUPG_002330"
     CDS             7090..7461
                     /gene="rplN"
                     /locus_tag="ACHUPG_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR0861926.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7472..7786
                     /gene="rplX"
                     /locus_tag="ACHUPG_002331"
     CDS             7472..7786
                     /gene="rplX"
                     /locus_tag="ACHUPG_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR0861927.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7801..8340
                     /gene="rplE"
                     /locus_tag="ACHUPG_002332"
     CDS             7801..8340
                     /gene="rplE"
                     /locus_tag="ACHUPG_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR0861928.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8355..8660
                     /gene="rpsN"
                     /locus_tag="ACHUPG_002333"
     CDS             8355..8660
                     /gene="rpsN"
                     /locus_tag="ACHUPG_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR0861929.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8694..9086
                     /gene="rpsH"
                     /locus_tag="ACHUPG_002334"
     CDS             8694..9086
                     /gene="rpsH"
                     /locus_tag="ACHUPG_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR0861930.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9099..9632
                     /gene="rplF"
                     /locus_tag="ACHUPG_002335"
     CDS             9099..9632
                     /gene="rplF"
                     /locus_tag="ACHUPG_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR0861931.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9642..9995
                     /gene="rplR"
                     /locus_tag="ACHUPG_002336"
     CDS             9642..9995
                     /gene="rplR"
                     /locus_tag="ACHUPG_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR0861932.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10010..10513
                     /gene="rpsE"
                     /locus_tag="ACHUPG_002337"
     CDS             10010..10513
                     /gene="rpsE"
                     /locus_tag="ACHUPG_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR0861933.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10517..10696
                     /gene="rpmD"
                     /locus_tag="ACHUPG_002338"
     CDS             10517..10696
                     /gene="rpmD"
                     /locus_tag="ACHUPG_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR0861934.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10700..11134
                     /gene="rplO"
                     /locus_tag="ACHUPG_002339"
     CDS             10700..11134
                     /gene="rplO"
                     /locus_tag="ACHUPG_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR0861935.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11142..12473
                     /gene="secY"
                     /locus_tag="ACHUPG_002340"
     CDS             11142..12473
                     /gene="secY"
                     /locus_tag="ACHUPG_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR0861936.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12505..12621
                     /gene="rpmJ"
                     /locus_tag="ACHUPG_002341"
     CDS             12505..12621
                     /gene="rpmJ"
                     /locus_tag="ACHUPG_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR0861937.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12768..13124
                     /gene="rpsM"
                     /locus_tag="ACHUPG_002342"
     CDS             12768..13124
                     /gene="rpsM"
                     /locus_tag="ACHUPG_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR0861938.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13141..13530
                     /gene="rpsK"
                     /locus_tag="ACHUPG_002343"
     CDS             13141..13530
                     /gene="rpsK"
                     /locus_tag="ACHUPG_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR0861939.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13564..14184
                     /gene="rpsD"
                     /locus_tag="ACHUPG_002344"
     CDS             13564..14184
                     /gene="rpsD"
                     /locus_tag="ACHUPG_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR0861940.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14210..15199
                     /gene="rpoA"
                     /locus_tag="ACHUPG_002345"
     CDS             14210..15199
                     /gene="rpoA"
                     /locus_tag="ACHUPG_002345"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR0861941.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15240..15623
                     /gene="rplQ"
                     /locus_tag="ACHUPG_002346"
     CDS             15240..15623
                     /gene="rplQ"
                     /locus_tag="ACHUPG_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR0861942.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4044 a         3691 c         4185 g         3788 t
ORIGIN      
        1 cgcagttcta cttccgtact actgacgtga ctggtaccat cgaactgccg gaaggcgtag
       61 agatggtaat gccgggcgac aacatcaaaa tggttgttac cctgatccac ccgatcgcga
      121 tggacgacgg tctgcgtttc gcaatccgtg aaggcggccg taccgttggc gcgggcgttg
      181 ttgctaaagt tctgggctaa ttacacgtta attagttttg aattgaaaag ggcgcttcgg
      241 cgcccttttt gctgcccgta atttgagctc gaaatattta gtcgtaattt tgctcgctga
      301 actatggcgc aaataaaact gtgctattgt aatcataacc attctcattt acactttgtg
      361 cagaatttga acgggagcaa tcatgtacgt ttgtttgtgt aatggtgtaa gtgacaaaaa
      421 aattcgccag gcagtccgtc agtttcatcc gcaatctttc caacaattgc gtaagtttat
      481 tcctgtggga aatcaatgcg gtaagtgcat tcgcgccgcg cgcgaagtga tgcaggacga
      541 gttgatgcag atgccagaat ttaaagagat tgcctgaagc ccacgcttat tttttgacat
      601 cctgctagcc ccatctacgc tctaatagtg gaagcggagg gactataaaa tgaaaggtga
      661 tgttaaaatc ataaattatc tcaataaact attgggaaat gagcttgtcg caatcaatca
      721 gtattttctc catgcccgga tgtttaaaaa ctggggcctg atgcgtctca atgacgtcga
      781 ataccatgaa tccatcgatg agatgaaaca cgctgataaa tatatcgagc gtattttgtt
      841 tttagaagga atccccaact tacaggattt aggcaaactg gggattggtg aagatgtcga
      901 ggagatgctg caatctgacc tgcgattaga actggaaggc gcgaaggatc tgcgtgaggc
      961 tatcgcatat gccgacagcg ttcatgacta tgttagccgc gatatgatga tcgagatcct
     1021 tgctgatgaa gaaggtcata tcgactggct cgaaactgaa ctggatctca tcggtaaaat
     1081 cggcctacaa aattacctgc aatcacaaat taaagttaaa gattaacggc ctatcagcaa
     1141 actatcccag cctataacga aacccgcagc cgccagaaat ggcccaaaag gcagcgggtt
     1201 ttttaatgat tcttttcctc tcatgagcag accaataact acggctccgc aagcgaatga
     1261 ggcagcaagg aaaaccagtc gtggtaagaa ggcccatgaa tgctaggcgc caagggcggc
     1321 gagaaacttc acatcaccat agcctaatcc ttctttatgt cgcagtatgc gataccccca
     1381 gtaaatgaca gcgaatgtgc cgtaaccaac aattgccccc cataacgcat cggctaaacc
     1441 atcaggatga caaacttgat agaacaaaag acctgaccag agtaacgggc acgtaaatct
     1501 gtcaggtaac aagccatgct ttgcatccca gaaaaaaagt agggctgata aacaagcgta
     1561 caaaattaga aagggaaggg tagtgtccat aaaacatcct ctgtaattgg agtatcacca
     1621 tcgaataact ccggatgctc agcaacagag agagcgctga tttgttaacc tgattccggt
     1681 gaaaaacgct atcttcacaa ccttacaaat ttcgtggaat gcagctcgca atctgcgcgt
     1741 tgcgattaat ttctacccga tgcttgcgtc aggctcagat ttgcgtataa tgcgcgggct
     1801 tgtcgtagtt gacagcaggt tcaatctgaa cctcagttag ccgaatttgg ctacctaaca
     1861 atgctcccaa tcggggagct acgtaagaac ggttacactc tcccatcaat cgtaatgggt
     1921 ctgaggagta atcattttcg tttataaaat aattggagct ctggtctcat gcagaaccaa
     1981 agaatccgta tccgcctgaa agcgtttgat catcgtctga tcgatcaagc aaccgcggaa
     2041 atcgtcgaga ctgccaagcg cactggtgcg caggtccgtg gtccgatccc gctgccgaca
     2101 cgcaaagagc gcttcactgt tctgatctcc ccgcacgtca acaaagacgc gcgcgatcag
     2161 tacgaaatcc gtactcactt gcgtctggtt gacatcgttg agccaaccga gaaaaccgtt
     2221 gatgctctga tgcgtctgga tctggctgcc ggtgtagacg tgcagatcag cctgggttaa
     2281 tcaggtcatt gagcgattga gaggttgaaa caatgattgg tttagtcggt aaaaaagtgg
     2341 gtatgacccg tatcttcaca gaagacggcg tttctatccc agtaaccgta atcgaagttg
     2401 aagcaaaccg cgttactcag gttaaagacc tggctaacga tggctaccgt gctattcagg
     2461 tgaccaccgg tgctaaaaaa gctaaccgtg tgaccaagcc tgaagctggc cacttcgcta
     2521 aagctggcgt agaagctggc cgtggtctgt gggaattccg cctggctgaa ggcgaagagt
     2581 tcactgtagg tcagagcatt agcgttgaac tgtttgctga cgttaaaaaa gttgacgtaa
     2641 ctggcacctc taaaggtaaa ggtttcgcag gtaccgttaa gcgctggaac ttccgtaccc
     2701 aggacgctac tcacggtaac tccttgtctc accgcgttcc gggttctatc ggtcagaacc
     2761 agactccggg caaagtgttc aaaggcaaga aaatggcagg tcagatgggt aacgaacgtg
     2821 taaccgttca gagccttgac gtagtacgcg ttgacgctga gcgcaacctg ctgctggtta
     2881 aaggtgctgt cccgggtgca accggtagcg acctgatcgt taaaccagct gtgaaggcgt
     2941 aaggagatag caatggaatt agtattgaaa gacgcgcaga gcgcgctgac tgtttccgaa
     3001 actaccttcg gtcgtgattt caacgaagcg ctggttcacc aggttgttgt tgcttatgca
     3061 gctggtgctc gtcagggtac tcgtgctcag aagactcgtg ctgaagtaac tggttccggt
     3121 aaaaaaccgt ggcgccagaa aggcaccggc cgtgcgcgtt ctggttctat caagagcccg
     3181 atctggcgtt ctggtggcgt gacctttgct gctcgtccgc aggaccacag tcaaaaagtt
     3241 aacaagaaga tgtaccgcgg cgcgctgaaa agcatcctgt ccgaactggt acgtcaggat
     3301 cgtctgatcg ttgtcgagaa gttctctgta gaagcgccga aaactaagct gctggcacag
     3361 aaactgaaag acatggctct ggaagatgtg ctgatcatca ccggtgagct ggacgaaaac
     3421 ctgttcctgg ctgcgcgcaa cctgcagaag gttgacgtac gcgatgcaac tggtatcgac
     3481 ccggttagcc tgatcgcctt cgacaaagtc gtaatgactg ctgatgctgt taagcaagtt
     3541 gaggagatgc tggcatgatt cgtgaagaac gtctgctgaa ggtgctgcgt gcaccgcacg
     3601 tttctgaaaa agcgtctact gcgatggaaa aatccaacac catcgtactc aaagttgcta
     3661 aagacgcgac caaagcagaa atcaaagctg ctgtgcagaa actgtttgaa gtcgaagtcg
     3721 aagtcgttaa caccctggta gttaaaggga aagttaaacg tcacggacag cgtatcggtc
     3781 gtcgtagcga ctggaaaaaa gcttacgtca ccctgaaaga aggccagaat ctggacttcg
     3841 ttggcggcgc tgagtaagtc ggaggagtaa tacaatggca gttgttaaat gtaaaccgac
     3901 atctccgggt cgtcgccacg tagttaaagt ggttaaccct gagctgcaca agggcaaacc
     3961 ttttgctccg ttgctggaaa aaaacagcaa atccggtggt cgtaacaaca atggccgtat
     4021 caccactcgt catatcggtg gtggccacaa gcaggcttac cgtattgttg acttcaaacg
     4081 caacaaagac ggtatcccgg cagttgttga acgtcttgag tacgatccga accgttccgc
     4141 gaacatcgcg ctggttctgt acaaagacgg tgaacgccgt tacatcctgg cccctaaagg
     4201 cctgaaagct ggcgaccaga ttcagtctgg cgttgatgct gcaatcaaac caggtaacac
     4261 cctgccgatg cgcaacatcc cggttggttc tactgttcat aacgtagaaa tgaaaccagg
     4321 taaaggcggt cagctggcac gttccgctgg tacttacgtt cagatcgttg ctcgtgatgg
     4381 tgcttatgtc accctgcgtc tgcgttctgg tgaaatgcgt aaagtagaag cagactgccg
     4441 tgcaactctg ggcgaagttg gcaatgctga gcatatgctg cgcgttctgg gtaaagcagg
     4501 tgctgcacgc tggcgtggtg ttcgtccgac cgttcgcggt accgcgatga acccggtaga
     4561 ccacccacat ggtggtggtg aaggtcgtaa ctttggtaag cacccggtaa ctccgtgggg
     4621 cgttcagacc aaaggtaaga agacccgcag caacaagcgt actgataaat tcatcgtacg
     4681 tcgccgtagc aaataatttt agaggataag ccatgccacg ttctctcaag aaaggtcctt
     4741 ttattgacct gcacttgctg aagaaggtag agaaagcggt ggaaagcgga gacaagaagc
     4801 ccctgcgcac ttggtcccgt cgttcaacga tctttcctaa catgatcggt ttgaccatcg
     4861 ctgtccataa tggtcgtcag cacgttccgg tatttgtaac cgacgaaatg gtcggtcaca
     4921 aactgggtga attcgcaccg actcgtactt atcgcggcca cgctgctgat aaaaaagcga
     4981 agaagaaata aggtaggagg aagagatgga aactattgct aaacatcgcc atgctcgttc
     5041 ttctgctcag aaggttcgcc ttgttgctga cctgattcgc ggtaagaaag tgtcgcaggc
     5101 tctggatatt ttgacctaca ccaacaagaa agcggctgta caggtcaaga aagttctgga
     5161 atctgccatt gctaacgctg aacacaacga tggcgctgac attgacgatc tgaaagttac
     5221 gaaaattttc gtagacgaag gcccgagcat gaagcgcatt atgccgcgtg caaaaggtcg
     5281 tgcagatcgc atcctgaagc gcaccagcca catcactgtg gttgtgtccg atcgctgaga
     5341 ctctggagac tagcaatggg tcagaaagta catcctaatg gtattcgctt gggtattgta
     5401 aaaccatgga actctacttg gtttgcgaac accaaagaat tcgctgacaa cctggacagc
     5461 gattttaaag tacgtcagta cctgactaag gaactggcta aagcgtccgt atctcgtatc
     5521 gttatcgagc gtccggctaa gagcatccgt gtaaccattc acactgctcg cccgggtatc
     5581 gttatcggta aaaaaggtga agacgtagaa aaactgcgta aggtcgtagc ggacatcgct
     5641 ggcgttcctg cacagatcaa catcgccgaa gttcgtaagc ctgaactgga cgcaaaactg
     5701 gttgctgaca gcatcacttc tcagctggaa cgtcgcgtta tgttccgtcg tgctatgaag
     5761 cgtgctgtac agaacgcaat gcgtctgggc gctaaaggta ttaaagttga agttagcggc
     5821 cgtctgggcg gcgcggaaat cgcacgtacc gaatggtacc gcgaaggtcg cgtaccgctg
     5881 cacactctgc gtgctgacat cgactacaac acctctgaag cgcacaccac ttacggtgta
     5941 atcggcgtta aagtgtggat cttcaaaggc gagatcctgg gtggtatggc tgctgttgaa
     6001 caaccggaaa aaccggctgc tcagcctaaa aagcagcagc gtaaaggccg taaataagga
     6061 gcgtcgctga tgttacaacc aaagcgtaca aaattccgta aaatgcacaa aggccgtaac
     6121 cgcggtctgg cgcagggtac ggatgttagc ttcggcagct tcggtctgaa agctgttggc
     6181 cgtggtcgtc tgactgcccg tcagatcgaa gcagcacgtc gtgctatgac ccgtgcagtt
     6241 aagcgtcaag gtaagatctg gatccgtgtg ttcccggaca aaccgatcac tgaaaagccg
     6301 ctggcagtgc gtatgggtaa aggtaaaggt aacgtggagt attgggttgc cttgattcag
     6361 ccgggtaaag tcctgtatga aatggacggt gttccggaag agctggcccg tgaagcattc
     6421 aagctggcag cagcgaaact gccgattaaa accacctttg taactaagac ggtgatgtaa
     6481 tgaaagcaaa agagctgcgt gagaagagca ttgaagagct gaacaccgag ctgctgaacc
     6541 tgctgcgtga gcagttcaac ctgcgtatgc aggctgcaag tggccagctg caacagtctc
     6601 acctgttgaa gcaagtgcgt cgcgatgtcg cacgcgttaa gactttactg aacgagaagg
     6661 cgggtgcgta atgaccgata aaatccgtac tctgcaaggt cgcgttgtta gcgacaaaat
     6721 ggagaaatcc attgttgttg ctatcgaacg ttttgtgaaa cacccgatct acggaaaatt
     6781 catcaagcgt acgaccaaac tgcacgtaca tgacgagaac aacgaatgcg gtatcggtga
     6841 cgtggttgaa atccgcgaat gccgtccgct gtccaagact aaatcctgga cgctggttcg
     6901 cgttgtagag aaagcggttc tgtaatacag tacactctct cgatacgaat aaacggctca
     6961 gaaatgagcc gtttattttt tctacccata tccttgaagc ggtgttataa tgccgcgccc
     7021 tcgatatggg gatttttaac gacctgattt tcgggtctca gtagtagttg acattagcgg
     7081 agcactaaaa tgatccaaga acagactatg ctgaacgtcg ccgacaactc cggtgcacgt
     7141 cgcgtaatgt gtatcaaggt tctgggtggc tcgcaccgtc gctacgcagg cgtaggcgac
     7201 atcatcaaga tcaccatcaa agaagcaatt ccgcgtggta aggtcaaaaa aggtgatgtg
     7261 ctgaaggcgg tagtggtgcg caccaagaag ggtgttcgtc gcccggacgg ttctgtcatt
     7321 cgcttcgatg gtaatgcttg tgttcttctg aacaacaaca gcgagcagcc tatcggtacg
     7381 cgtatttttg ggccggtaac tcgtgagctt cgtagtgaga agttcatgaa aattatctct
     7441 ctggcaccag aagtactcta aggagcgaat catggcagcg aaaatccgtc gtgatgacga
     7501 agttatcgtg ttaaccggta aagataaagg taaacgcggt aaagttaaga atgtcctgtc
     7561 ttccggcaag gtcattattg aaggtatcaa cctggttaag aaacatcaga agccggttcc
     7621 ggccctgaac caaccgggtg gcatcgttga aaaagaagcc gctattcagg tttccaacgt
     7681 agcaatcttc aatgcggcaa ccggcaaggc tgaccgtgta ggctttagat tcgaagacgg
     7741 caaaaaagtc cgtttcttca agtctaacag cgaaactatc aagtaatttg gagtagtacg
     7801 atggcgaaac tgcatgatta ctacaaagac gaagtagtta aaaaactcat gactgagttt
     7861 aactacaatt ctgtcatgca agtccctcgg gtcgagaaga tcaccctgaa catgggtgtt
     7921 ggtgaagcga tcgctgacaa aaaactgctg gataacgcag cagcagacct ggcagcaatc
     7981 tccggtcaaa aaccgctgat caccaaagca cgcaaatctg ttgcaggctt caaaatccgt
     8041 cagggctatc cgatcggctg taaagtaact ctgcgtggcg aacgcatgtg ggagttcttt
     8101 gagcgcctga tcactattgc tgtacctcgt atccgtgact tccgtggcct gtccgctaag
     8161 tctttcgacg gtcgtggtaa ctacagcatg ggtgtccgtg agcagatcat cttcccagaa
     8221 atcgactacg ataaagtcga ccgcgttcgt ggtttggata ttaccattac cactactgcg
     8281 aaatctgacg aagaaggccg cgctctgctg gctgcctttg acttcccgtt ccgcaagtaa
     8341 ggtagggtta ctaaatggct aagcaatcaa tgaaagcacg cgaagtaaaa cgcgtagctt
     8401 tagctgataa atacttcgcg aaacgcgctg aactgaaagc gatcatctct gatgtgaacg
     8461 cttccgacga agatcgttgg aacgctgttc tcaagctgca gactctgccg cgtgattcca
     8521 gcccgtctcg tcagcgtaac cgctgccgtc aaacaggtcg tccgcatggt ttcctgcgga
     8581 agttcgggtt gagccgtatt aaggtccgtg aagccgctat gcgcggtgaa atcccgggtc
     8641 tgaaaaaggc tagctggtaa ttgtcaccaa ttgaatcacg ggagtaaaga cagatgagca
     8701 tgcaagatcc gatcgcggat atgctgaccc gtatccgtaa cggtcaggcc gcgaacaaag
     8761 ctgcggtcac catgccttcc tccaagctga aagtggcaat cgccaacgtg ctgaaggaag
     8821 aaggttttat tgaagatttt aaagttgaag gcgacaccaa gcctgaactg gaacttactc
     8881 tgaagtattt ccagggcaaa gctgttgtag aaagcattca gcgtgtcagc cgcccaggtc
     8941 tgcgcatcta taaacgtaaa gatgagctgc cgaaagttat ggcgggtctg ggtatcgcag
     9001 ttgtttctac ctctaaaggt gttatgactg atcgtgcagc gcgccaggct ggtcttggtg
     9061 gcgaaattat ctgctacgta gcctaatcgg aggaaaaaat gtctcgtgtt gctaaagcac
     9121 cggtcgttgt tcctgccggc gttgacgtaa aaatcaacgg tcaggttatt acgatcaaag
     9181 gtaaaaacgg cgagctgact cgtactctca acgatgctgt tgaagttaaa catgcagata
     9241 ataccctgac cttcggtccg cgtgatggtt acgcagacgg ttgggcacag gctggtaccg
     9301 cgcgtgccct gctgaactca atggttatcg gtgttaccga aggcttcact aagaagctgc
     9361 agctggttgg tgtaggttac cgtgcagcgg ttaaaggcaa tgtgattaac ctgtctctgg
     9421 gtttctctca tcctgttgac catcagctgc ctgcgggtat cactgctgaa tgtccgactc
     9481 agactgaaat cgtgctgaaa ggcgctgata agcaggtgat cggccaggtt gcagcggatc
     9541 tgcgcgccta ccgtcgtcct gagccttaca aaggcaaggg ggttcgttac gccgacgaag
     9601 tcgtgcgtac caaagaggct aagaagaagt aaggtaacac tatggataag aaatctgctc
     9661 gtatccgtcg tgcgacccgc gcacgccgca agctccagga gctgggcgca actcgcctgg
     9721 tggtacatcg taccccgcgt cacatttacg cacaggtaat tgcaccgaac ggttctgaag
     9781 ttctggtagc tgcttctact gtagaaaaag ctatcgctga acaactgaag tacaccggta
     9841 acaaagacgc ggctgcagct gtgggtaaag ctgtcgctga acgcgctctg gaaaaaggca
     9901 tcaaagatgt atcctttgac cgttccgggt tccaatatca tggtcgtgtc caggcactgg
     9961 cagatgctgc ccgtgaagct ggccttcagt tctaaggtag aggtgtaaga tggctcacat
    10021 cgaaaaacaa gctggcgaac tgcaggaaaa gctgatcgcg gtaaaccgcg tatctaaaac
    10081 cgttaaaggt ggtcgtattt tctccttcac agctctgact gtagttggcg atggtaacgg
    10141 tcgcgttggt tttggttacg gtaaagcgcg tgaagttcca gcagcgatcc agaaagcgat
    10201 ggaaaaagcc cgtcgcaata tgattaacgt cgcgctgaat aacggcactc tacaacaccc
    10261 tgttaaaggt gttcacacgg gttctcgcgt attcatgcag ccggcttccg aaggtaccgg
    10321 tatcatcgcc ggtggtgcaa tgcgcgccgt tctggaagtc gctggggttc ataacgttct
    10381 ggctaaagca tatggttcca ccaacccgat caacgtggtt cgtgcaacta ttgatggcct
    10441 ggaaaatatg aattctccag aaatggtcgc tgccaagcgt ggtaaatccg ttgaagaaat
    10501 tctggggaaa taaaccatgg caaagactat taaaattact caaacccgca gtgcaatcgg
    10561 tcgtctgccg aaacacaagg caacgctgct tggcttgggt ctgcgtcgta ttggtcacac
    10621 cgtagagcgc gaggatactc ctgctattcg cggtatgatc aacgcggttt ccttcatggt
    10681 taaagttgag gagtaagaga tgcgtttaaa tactctgtct ccggccgaag gctccaaaaa
    10741 ggcgggtaaa cgcctgggtc gtggtatcgg ttctggcctc ggtaaaaccg gtggtcgtgg
    10801 tcacaaaggt cagaagtctc gttctggcgg tggcgtacgt cgcggtttcg agggtggtca
    10861 gatgcctctg taccgtcgtc tgccgaaatt cggcttcact tctcgtaaag cagcgattac
    10921 agccgaagtt cgtctatctg acttggctaa agtagaaggc ggtgttgtag acctgaacac
    10981 gctgaaagcg gctaacatta tcggtatcca gatcgagttc gcgaaagtga tcctggctgg
    11041 cgaagtaacg actccggtaa ctgttcgtgg cctgcgtgtt actaaaggcg ctcgtgctgc
    11101 tatcgaagct gctggcggta aaatcgagga ataagtagca gatggctaaa caaccgggat
    11161 tagattttca aagtgccaaa ggtggcttag gcgagctgaa acgcagactg ctgtttgtta
    11221 tcggtgcgct gattgtgttc cgtattggct cttttattcc gatccctggt attgatgccg
    11281 ctgtacttgc caaactgctt gagcaacagc gaggcaccat cattgagatg tttaacatgt
    11341 tctctggtgg tgctctcagc cgtgcttcta tctttgctct ggggatcatg ccgtatattt
    11401 cggcgtcgat cattatccag ctgctgacgg tggttcaccc aacgttggca gaaattaaga
    11461 aagaagggga gtctggtcgt cgtaagatca gccagtacac ccgttacggt actctggtgc
    11521 tggcaatatt ccagtcgatc ggtattgcta ccggtctgcc gaatatgcct ggtatgcaag
    11581 gcctggtgat taacccgggc tttgcattct acttcaccgc tgttgtaagt ctggtcacag
    11641 gaacgatgtt cctgatgtgg ttgggcgaac agatcactga acgaggtatc ggcaacggta
    11701 tttcaatcat tatcttcgcc ggtattgtcg cgggactccc gccagccatt gcccatacta
    11761 tcgagcaagc gcgtcaaggc gacctgcact tcctcgtgtt gctgttggtt gcagtattag
    11821 tatttgcagt gacgttcttt gttgtgtttg ttgagcgtgg tcaacgccgc attgtggtaa
    11881 actacgcgaa acgtcagcaa ggtcgtcgtg tctatgctgc acagagcaca catttaccgc
    11941 tgaaagtgaa tatggcgggg gtaatcccgg caatcttcgc ttccagtatt attctgttcc
    12001 cggcgaccat cgcgtcatgg ttcgggggcg gtactggttg gaactggctg acaacaattt
    12061 cgctgtattt gcagcctggg caaccgcttt atgtgttact ctatgcgtct gcaatcatct
    12121 tcttctgttt cttctacacg gcgttggttt tcaacccgcg tgaaacagca gataacctga
    12181 agaagtccgg tgcatttgta ccaggaattc gtccgggaga gcaaacggcg aagtatatcg
    12241 ataaagtaat gacccgcctg accttggttg gtgcgctgta tattaccttt atctgcctga
    12301 tcccggagtt catgcgtgat gcaatgaaag taccgttcta cttcggtggg acctcactgc
    12361 ttatcgttgt tgtcgtgatt atggacttta tggctcaagt gcaaactctg atgatgtcca
    12421 gtcagtatga gtctgcattg aagaaggcga acctgaaagg ctacggccga taattggtcg
    12481 cccgagaagt tacggagagt aaaaatgaaa gttcgtgctt ccgtcaagaa attatgccgt
    12541 aactgcaaaa tcgttaagcg tgatggtgtc atccgtgtga tttgcagtgc cgagccgaag
    12601 cataaacagc gccaaggctg attttttcgc atatttttct tgcaaagttg ggttgagctg
    12661 gctagattag ccagccaatc ttttgtatgt ctgtacgttt ccatttgagt atcctgaaaa
    12721 cgggcttttc agcatggaac gtacatatta aatagtagga gtgcatagtg gcccgtatag
    12781 caggcattaa cattcctgat cataagcatg ccgtaatcgc attaacttcg atttatggcg
    12841 tcggcaagac ccgttctaaa gccatcctgg ctgcagcggg tatcgctgaa gatgttaaga
    12901 tcagtgagct gtctgaagga caaatcgaca cgctgcgtga cgaagttgcc aaatttgtcg
    12961 ttgaaggtga tctgcgccgt gaaatcagca tgagcatcaa gcgcctgatg gatcttggtt
    13021 gctatcgcgg tttgcgtcat cgtcgtggtc tcccggttcg cggtcagcgt accaagacca
    13081 acgcacgtac ccgtaagggt ccgcgcaaac cgatcaagaa ataatcgggg tgattgaata
    13141 atggcaaagg caccaattcg tgcacgtaaa cgtgtaagaa aacaagtctc tgacggcgtg
    13201 gctcatatcc atgcttcttt caacaacacc atcgtgacta tcactgatcg tcagggtaac
    13261 gcgttgggtt gggcaacagc cggtggttcc ggtttccgtg gttctcgcaa atccactccg
    13321 tttgcagctc aggttgcagc agagcgttgc gctgacgccg tgaaagaata cggcatcaag
    13381 aatctggaag ttatggttaa aggtccgggt ccaggccgcg aatctactat tcgtgctctg
    13441 aacgccgcag gtttccgcat cactaacatt actgatgtga ctccgatccc tcataacggt
    13501 tgtcgtccgc cgaaaaaacg tcgcgtataa cgcctcgttt tccaggtttg ttggagaaag
    13561 aaaatggcaa gatatttggg tcctaagctc aagctgagcc gtcgtgaggg caccgactta
    13621 ttccttaagt ctggcgttcg cgcgatcgat accaagtgta aaattgaaca agctcctggc
    13681 cagcacggtg cgcgtaaacc gcgtctgtct gactatggtg tgcagttgcg tgaaaagcaa
    13741 aaagttcgcc gtatctatgg tgtgctggag cgtcagttcc gtaactacta caaagaagca
    13801 gcacgtctga aaggcaacac cggtgaaaac ctgttggctc tgctggaagg tcgtctggac
    13861 aacgttgtat accgtatggg cttcggtgcc actcgtgcag aagcacgtca gctggttagc
    13921 cataaagcaa ttatggtaaa cggtcgtgtt gttaacatcg cttcttatca ggttagtccg
    13981 aatgacgttg taagcattcg tgagaaagcg aagaagcagt ctcgcgtgaa agccgctctg
    14041 gagctggctg agcagcgtga aaagccaact tggctggaag ttgatgctgg caagatggaa
    14101 ggtacgttta agcgtaagcc ggagcgttct gatctgtctg cggacattaa cgaacacctg
    14161 atcgtcgagc tttactccaa gtaaagctta gtaccaaaga gaggacacaa tgcagggttc
    14221 tgtgacagag tttctaaaac cgcgcctggt tgatatcgag caagtgagtt cgacgcacgc
    14281 caaggtgacc cttgagcctt tagagcgtgg ctttggccat actctgggta acgcactgcg
    14341 ccgtattctg ctctcatcga tgccgggttg cgcggtgacc gaggttgaga ttgatggtgt
    14401 actacatgag tacagcacca aagaaggcgt tcaggaagat atcctggaaa tcctgctcaa
    14461 cctgaaaggg ctggcggtga gagttcaggg caaagatgaa gttattctta ccttgaataa
    14521 atctggcatt ggccctgtga ctgcagccga tatcacccac gacggtgatg ttgaaatcgt
    14581 caagccgcag cacgtgatct gccacctgac cgatgagaac gcgtctatta gcatgcgtat
    14641 caaagttcag cgcggtcgtg gttatgtgcc ggcttctacc cgaattcatt cggaagaaga
    14701 tgagcgccca atcggccgtc tgctggtcga cgcatgctac agccctgtgg agcgtattgc
    14761 ctacaatgtt gaagcagcgc gtgtagaaca gcgtaccgac ctggacaagc tggtcatcga
    14821 aatggaaacc aacggcacaa tcgatcctga agaggcgatt cgtcgtgcgg caaccattct
    14881 ggctgaacaa ctggaagctt tcgttgactt acgtgatgta cgtcagcctg aagtgaaaga
    14941 agagaaacca gagttcgatc cgatcctgct gcgccctgtt gacgatctgg aattgactgt
    15001 ccgctctgct aactgcctta aagcagaagc tatccactat atcggtgatc tggtacagcg
    15061 taccgaggtt gagctcctta aaacgcctaa ccttggtaaa aaatctctta ctgagattaa
    15121 agacgtgctg gcttcccgtg gactgtctct gggcatgcgc ttggaaaact ggccaccggc
    15181 aagcatcgct gacgagtaac cggatcacag gttaaggttt tactgagaag gataaggtca
    15241 tgcgccatcg taagagtggt cgtcaactga accgcaacag cagccatcgc caggctatgt
    15301 tccgcaatat ggcaggttca ctggttcgtc atgaaatcat caagacgact ctgcctaaag
    15361 cgaaagagct gcgccgcgta gttgagccgc tgattactct tgccaagact gatagcgttg
    15421 ctaatcgtcg tctggcattc gcccgtactc gtgataacga gatcgtggca aaactgttta
    15481 acgaactggg cccgcgtttc gcgagccgtg ccggtggtta cactcgtatt ctgaagtgtg
    15541 gcttccgtgc aggcgacaac gcgccgatgg cttacatcga gctggttgat cgttcagaga
    15601 aagcagaagc tgctgcagag taatctgaag caatgtaaaa aaacccgccc cggcgggttt
    15661 ttttataccc gtagtatccc cacttatcta caatagctgt actctttt
//
LOCUS       DAZPAB010000084        15610 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZPAB010000084 DAZPAB010000000
VERSION     DAZPAB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15610
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.084"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            53..571
                     /locus_tag="ACHUPG_002347"
     CDS             53..571
                     /locus_tag="ACHUPG_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR0861943.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            561..1786
                     /gene="dgcN"
                     /locus_tag="ACHUPG_002348"
                     /pseudo
     CDS             561..1786
                     /gene="dgcN"
                     /locus_tag="ACHUPG_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            1802..2284
                     /locus_tag="ACHUPG_002349"
     CDS             1802..2284
                     /locus_tag="ACHUPG_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR0861944.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(2361..2708)
                     /gene="rplS"
                     /locus_tag="ACHUPG_002350"
     CDS             complement(2361..2708)
                     /gene="rplS"
                     /locus_tag="ACHUPG_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR0861945.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2750..3517)
                     /gene="trmD"
                     /locus_tag="ACHUPG_002351"
     CDS             complement(2750..3517)
                     /gene="trmD"
                     /locus_tag="ACHUPG_002351"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR0861946.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3548..4096)
                     /gene="rimM"
                     /locus_tag="ACHUPG_002352"
     CDS             complement(3548..4096)
                     /gene="rimM"
                     /locus_tag="ACHUPG_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR0861947.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4115..4363)
                     /gene="rpsP"
                     /locus_tag="ACHUPG_002353"
     CDS             complement(4115..4363)
                     /gene="rpsP"
                     /locus_tag="ACHUPG_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR0861948.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4500..5861)
                     /gene="ffh"
                     /locus_tag="ACHUPG_002354"
     CDS             complement(4500..5861)
                     /gene="ffh"
                     /locus_tag="ACHUPG_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR0861949.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6028..6819
                     /locus_tag="ACHUPG_002355"
     CDS             6028..6819
                     /locus_tag="ACHUPG_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR0861950.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6840..8126
                     /locus_tag="ACHUPG_002356"
     CDS             6840..8126
                     /locus_tag="ACHUPG_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR0861951.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8181..8774)
                     /gene="grpE"
                     /locus_tag="ACHUPG_002357"
     CDS             complement(8181..8774)
                     /gene="grpE"
                     /locus_tag="ACHUPG_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR0861952.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            8897..9775
                     /gene="nadK"
                     /locus_tag="ACHUPG_002358"
     CDS             8897..9775
                     /gene="nadK"
                     /locus_tag="ACHUPG_002358"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR0861953.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            9861..11522
                     /gene="recN"
                     /locus_tag="ACHUPG_002359"
     CDS             9861..11522
                     /gene="recN"
                     /locus_tag="ACHUPG_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR0861954.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            11671..12012
                     /gene="bamE"
                     /locus_tag="ACHUPG_002360"
     CDS             11671..12012
                     /gene="bamE"
                     /locus_tag="ACHUPG_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR0861955.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12074..12364)
                     /locus_tag="ACHUPG_002361"
     CDS             complement(12074..12364)
                     /locus_tag="ACHUPG_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR0861956.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12354..12791)
                     /gene="ratA"
                     /locus_tag="ACHUPG_002362"
     CDS             complement(12354..12791)
                     /gene="ratA"
                     /locus_tag="ACHUPG_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR0861957.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            12962..13444
                     /gene="smpB"
                     /locus_tag="ACHUPG_002363"
     CDS             12962..13444
                     /gene="smpB"
                     /locus_tag="ACHUPG_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR0861958.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            13659..14021
                     /gene="ssrA"
                     /locus_tag="ACHUPG_002364"
     tmRNA           13659..14021
                     /gene="ssrA"
                     /locus_tag="ACHUPG_002364"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            14226..15467
                     /locus_tag="ACHUPG_002365"
     CDS             14226..15467
                     /locus_tag="ACHUPG_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFR0861959.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         3948 a         4110 c         3709 g         3843 t
ORIGIN      
        1 catcctgaaa aaatatcgat gcatttcgag cgaagatggt ggggatccct gaatgcgttt
       61 ttctcaccga ctgttcttac ttctgattct cctgctgacg ggagcaccaa tactcgctca
      121 agagccttct gacgttgcga aaaacgtgcg tatgatggtt tccggtattg tcagttacac
      181 ccgctggcct gcgctatcag ggccaccaaa gttatgtatc ttttcatctt cacgttttag
      241 taccgcccta caagagaatg ccgcgacatc tttgccttat ctgcctgtca tcattcacac
      301 tcaacaagag gcgatgattt caggctgcaa tggtttttat tttggcaacg agtcaccgac
      361 attccagatg gaattaaccg aacaatatcc gtcaaaggcg ttattattaa ttgccgaaca
      421 aaataccgag tgcattattg gtagcgcctt ttgtctgatc atccacaata atgacgtcag
      481 atttgccgta aacctggatg cctcatcgcg tagcggtgta aaggtcaacc cggatgtctt
      541 aatgctcgca cggaagaaaa atgatggata acgataattc tcttaataag cgccccacgt
      601 ttaaaagagc attacgcaac atcagtatga ccagcatatt tatcactatg atgctgatct
      661 ggttgctgct ttccgtgacc tcggtgctga ccctgaaaca gtacgcgcaa aaaaacctgg
      721 cactgacagc agcaacaatg acttacagtc tggaagcagc tgtcgttttt gccgatggcc
      781 ctgcagcaac tgaaacactg gcagcgctgg gccagcaagg gcattttcaa ctgcagaagt
      841 acgtgataag cagcaaaata ttctggcatc ctggcattac ccccgtaagg atccaggcga
      901 tactttcagc aatttcataa gccactggct cttccctgcc cccatcattc agccgattcg
      961 tcacaatggt gaaaccattg gcgaagtacg cttaaccgct cgcgacagtt caatcagcca
     1021 ttttatctgg ttttcgctcg ccgtactgac cggttgtatt ctgctggcat caggcatcgc
     1081 aattaccctc acccgccatt tgcacaatgg cctggtggaa gcactgaaaa atatcaccga
     1141 tgtcgtacat gatgtgcgtt ccaaccgcaa tttttcccga cgagtttcgg aagaacgtat
     1201 cgctgagttt caccgcttcg ctctcgactt caacagtctg ctggatgaaa tggaagagtg
     1261 gcagcttcgt ttacaggcca aaaatgcgca gcttctgcgt accgcgctac atgacccatt
     1321 aaccgggctg gctaaccgcg cagcgtttcg tagcggcatc aatacgttga tgaacaattc
     1381 cgatgcccga aaaacgtcgg cgttactatt tcttgatggc gataatttca aatatatcaa
     1441 tgatacctgg ggtcatgcga cgggcgatag ggtcttgatt gaaatcgcaa aacggttagc
     1501 tgaatttggc gggctgcgac ataaagcata ccgcctgggc ggcgatgaat tcgctatggt
     1561 gctctatgat gtacagtcgg aatctgaagt gcagcagata tgctcagcac tgacacaaat
     1621 ctttaatctc ccgtttgatc ttcataatgg ccatcagacc accatgacat taagcattgg
     1681 ttacgcgatg accattgagc acgcctctgc ggaaaaatta caagagcttg ccgatcacaa
     1741 tatgtatcag gccaaacacc agcgtgccga aaagctggtg agataacaag gatatatcga
     1801 tatgataaag cacctggtag cacccctgat tttcacctca ctaatactga ctggctgcca
     1861 gtcccctcag ggaaagttta ctcctgagca agtcgccgct atgcaatctt atggatttac
     1921 tgaatccgcc ggcgactggt cgctgggctt atcagatgcc attctgttcg caaaaaatga
     1981 ctacaaattg ctcccggaaa gccagcaaca gatccaaacc atggcagcca aattggcctc
     2041 gacagggcta acacatgccc gtatggatgg acacaccgat aactatggtg aagacagtta
     2101 caacgaaggc ttatcattga aacgggcgaa tgtcgtggcc gatgcatggg ctattggtgg
     2161 acaaattcca cgcagcaatc tcaccacaca gggtttagga aaaaaatatc ccatagccag
     2221 taacaagacc gcccagggcc gcgccgagaa ccgccgcgtc gcagtggtga ttgcaacccc
     2281 ttaaaacacg agcaaacaga caaaaaaagg gccagccaat tggccagccc ttcttaacag
     2341 gatgtcgctt aagcgaaatc ttagttaaga cgctctttga tacgagcagc cttaccagta
     2401 cgctcacgca ggtagtacag tttagcttta cgaacagcac cacgacgttt gacagaaatg
     2461 ctgtcaacta ccggagagtg agtctggaag acacgctcaa cgccttcgcc gttggaaatt
     2521 ttacgaacag tgaatgcaga gtgcagaccg cggttacgaa tagcgataac cacgccctcg
     2581 aatgcctgca gacgtttttt ggaaccttca acaacccata ctttcacttc cacggtatca
     2641 cccggacgga aggaaggtac gtcctgcttc atctgctctt gttcaagttg cttaataatg
     2701 ttgctcataa tttaatctct tatcctgggt aaactgatct ctcgggggct tacgccatcc
     2761 catcatgttt atgttgctgt tgtgcgtgtt ccgttttgaa ctccgccagc aaccttgctt
     2821 gctcttcagt cagagccagg ttttccagaa gttcaggtct tctaagccag gtacggccca
     2881 gcgactgttt caaacgccag cgacgtatct cggcatggtt gcccgacagt aacactggcg
     2941 gaacttccat cccttctaac acctcaggcc gcgtatagtg cgggcaatcc agcaatcctt
     3001 cagcaaagga atcttccgtt gccgaggctt catgtcccag tactcccgga ataaaccggg
     3061 aaacggagtc aatcagcgtc attgctggta actcaccacc actgagaacg taatcgccga
     3121 ttgaccattc ttcgtcaatt tcggtttgga tcacgcgctc atctatacct tcgtagcgac
     3181 cgcacaccag aatcaatttt tgattcgttg ccagttcgct gacgcccgct tgatcaagct
     3241 tgcgtccctg tggtgacaga taaatcacct ttgcgccttc acccgccgcg gcttttgctg
     3301 catgaatggc gtcccgcaag ggttgcacca tcattaacat ccccggtccg ccgccgtaag
     3361 gacgatcgtc cacggtacgg tgccggtcat gcgtgaagtc gcgaggactc caactctgga
     3421 tgctcagcag gccattttta actgcccggc cagttacccc gtaatcggta attgcgcgga
     3481 acatttcagg aaacaggcta attatgccaa tccacatagc gccgcctttt accgtttatc
     3541 cggtggttta aaaaccagga tcccaatcta cttcgattga acgagtagtg agatcgactt
     3601 tcttgataac ctgcccatcg aggaacggta cgagacgttc cttgatacca aacgcatctt
     3661 tcaggtttgc cttaatgacg agaacgtcat tagatccggt ttccatcata tcaacgactt
     3721 taccgagatc gtagccttca gtggttacta cctggcagcc catcaggtct ttccagtagt
     3781 agtccccctc ttcaagctga ggcagctgcg atgaatccac gacaatttca caattcgtca
     3841 gcaggttcgc cgcatcacga tcgtcaacgc ctttcagctt gatgatcatg tcctgattgt
     3901 ggtgcttcca gctttccagc tggacttgct gccactgacc cgccttctgg ataaaccagg
     3961 gctgatagtc aaaaatgctt tcggcgtctt cggtggaaga aaacactctg agccacccac
     4021 gaataccgta agacgaaccc atttttccca aaacgatggg atcaacaggt gcttgcgcgg
     4081 tgagttgttt gctcatcatg accaccgtga cagattaagc tgctttgttt acttctttga
     4141 tcagcgcagc aacgcgatca gaaatagttg cgccctggcc aacccagtga gcgatgcgat
     4201 ccagatccag gcgagtgcct tcttcttttt cgctagcgat tgggttgaag aaaccaacgc
     4261 gctcgatgaa gcgaccgttg cgtgcattac ggctgtcagc gacaacaacc tggtagaacg
     4321 gacgcttttt agcgccgtga cgtgctaaac gaatagttac cataacatcc tcttgtgtga
     4381 ataaaacaac cggaccccat cgaggaacgg agtccggtgt catattaaaa gcccgaaaat
     4441 tttactgatt tctggagaaa atgcaatcag cagttaataa ctctgtcgca taaggtcatt
     4501 tagcgaccag gaaatcccgg cggcatcatt cccttcatgc ttctcatcat cttcgccatt
     4561 ccgcctttct tcattttctt catcatgcgc tgcatgtcgt cgaactgctt cagaaggcgg
     4621 ttaacgtcct gtacctgcat cccgcaaccg gcggcgatac gacgcttacg cgaacctttg
     4681 atgatttctg gcttagcgcg ctctttcatc gtcattgagt tgatgatggc ttccatacgc
     4741 accagcactt tatcgtccat ctgcgacttg acgttatccg ggatctgccc catgcccggc
     4801 agtttgccca tcaagctggc catgccgccc atatttttca tctggcgcag ttgctcaaga
     4861 aagtcgttga gatcgaagcc gtcgcctttt tttagcttgc tggctaattt ctctgcctgc
     4921 gcgcggtcaa ctttgctttc gatatcttcg atcagcgaca gtacgtcgcc catgccgaga
     4981 atacgcgacg cgatgcggtc cggatggaac ggctccagcg cctcagtttt ctcgccaaca
     5041 ccgaggaact tgatcggttt gccagtgatg tgacgaatag agagcgccgc accgccgcgg
     5101 gcatcgccgt ccactttggt caacactacg ccggtaagcg gtaacgcttc attgaatgct
     5161 tttgccgtat tggccgcatc ctgaccggtc atggcgtcaa ccacaaacag ggtttcaacc
     5221 gggttaatcg acgcatggac ttgtttgatc tcgtccatca tcgcttcgtc aacgtgcaga
     5281 cgaccagcgg tatccaccag cagcacgtcg tagaatttca gtttggcttc tttcagcgcc
     5341 gcgttaacaa tatcaaccgg cttctgacca acatcagaag ggaagaaatc aacgcccacc
     5401 tgctctgcca gcgtctcaag ctgtttgatt gccgccgggc gatagacgtc agcagaaacc
     5461 accagcactt tcttcttgtg cttctcgcgc aggaaattac cgagtttacc aacgctggtt
     5521 gttttaccgg caccttgcag gcctgccatc agcacgaccg caggcggttg cgcagccaga
     5581 ttcagggtct ggttctcttc gcccatcgcc gcaaccagtt cgttacggac gattttgacg
     5641 aactcctgcc ccggcgtcag gctcttatta acttcatgac caaccgcttt ctcttttacg
     5701 cgattgataa actcacgcac taccggcaga gcgacgtccg cctccagcag cgccatgcgc
     5761 acttcgcgca gcgtatcttt tacgttgtct tcagtgaggc gtccacggcc actgatattg
     5821 cgcagcgtgc gcgacaaacg atcggttaaa ttatcaaaca ttgtctctcg cctggggtgg
     5881 aaacggttgg ccgcaatcgc gacacatcat cagtattttg ccgcagtata acatgaaggc
     5941 gtctttgttg ttatgcaacg gttggagcag cgttcacctg acgctatact gcttctcttt
     6001 cttattgctc aaactgtcga cattactatg cccgtttttg ctctgctcgc gcttgtcgcc
     6061 tactccgtca gtcttgcgct gattgttccc ggtctgctgc aaaaaaacgg cggctggcgg
     6121 cgcatggcta ttatttctgc ggtcattgcg ctggtctgcc acgcaatcgc tctggaagcc
     6181 cgcatcctgc ccgacggtga tagcggacaa aacctcagcc tgctgaacgt tggttcattg
     6241 gtcagtttgg tgatctgtac ggtaatgacc attgtggctt ctcgcaatcg tggctggttg
     6301 ctgctaccca ttgtctatgc ctttgcgctt atcaacctgg cgctggcgac cttcatgccc
     6361 aatgaataca tcacccatct ggaagcaacg cctgggatgc tgatgcacat tggcttatcg
     6421 ctcttttcct atgccacgct aattatcgcc gccctgtacg cgctgcaact ggcgtggatt
     6481 gattaccaac tgaagaacaa gaagctggca tttaaccaag aaatgccgcc attgatgagt
     6541 atcgagcgta aaatgttcca catcacgcag attggcgtgg tgctgctaac gctcacgctt
     6601 tgcactggcc tgttctacat gcacaaccta tttagcatgg aaaatatcga caaggctgtg
     6661 ctctctatcg tggcgtggtt tgtctatatt gtgctgctgt ggggacatta ccatgaagga
     6721 tggcgtggac gccgcgtcgt ctggtttaac gttgcgggcg cggtaattct gacactggcc
     6781 tacttcggca gccgaattgt ccagcagtta atcagctaaa cccagaaaag gagtttcccc
     6841 tggaacacat ttctactact acgttgatca ttattctgat catcatggtg gtcatttcag
     6901 cctatttttc cgggtccgaa accggaatga tgaccctcaa ccgctatcgt cttcgacata
     6961 tggcgaaaca gggtaatcgc tcggccaaac gcgtcgaaaa attgctgcgt aagccagacc
     7021 gcctgataag cctggtgtta atcggcaata acctggtcaa tattcttgcc tccgcgctcg
     7081 gcactattgt cgggatgcgt ttgtacggcg atgcgggcgt ggcaattgcg actggtgtgc
     7141 tgacttttgt cgtactggtt tttgctgagg tattgccgaa aaccattgcc gcgctgtacc
     7201 cggaaaaagt cgcttatccg agtagttttc tgctggctcc gctgcaaatt ttgatgatgc
     7261 cgctggtctg gttgctgaat gctatcaccc gtatgctgat gcgcatgatg ggtatcaaaa
     7321 ccgatatcgt ggttagcggc tctttgagca aagaagagtt gcgcactatc gtgcatgaat
     7381 cgcgctcaca aatttcccgt cgcaatcagg atatgctgct gtcggtgctc gatctggaaa
     7441 aaatgaccgt tgatgacatc atggtaccgc gcagtgaaat tatcggtatt gatatcaacg
     7501 atgactggaa atctattctg cgccaactct cccactcacc tcacgggcgc atcgtgcttt
     7561 accgtgattc gctggacgac gccatcagta tgctgcgagt acgtgaagcc tggcggttga
     7621 tgtcggagaa aaaagagttc accaaagaaa ccatgctgcg cgccgcggac gagatctatt
     7681 ttgtgcctga aggtacgccg ctcagcacgc agttggtaaa gtttcagcgc aacaaaaaga
     7741 aagtcggcct ggtcgtcaac gagtatggag acattcaggg gctggtgacg gttgaagata
     7801 ttctggaaga gattgttggc gatttcacta cgtcgatgtc gccaacactt gccgaagagg
     7861 tcacgccgca aaacgacggt tcggtgatta tcgatggcac cgccaacgtg cgggaaatca
     7921 acaaagcctt taactggcat ctaccggaag atgatgcccg cacggttaat ggcgtcattc
     7981 ttgaggcact ggaagagatc cctgtcgcag gcacccgcgt gcgtattggc aagtacgata
     8041 tcgatattct cgacgtacag gacaatatga ttaagcaggt aaaagttttt cctgtgaaac
     8101 cgctgcgcga gagtgtggcg gagtaacgaa aacggccctg cattcgcatg cagggccgtg
     8161 aattattacg aaaacagaaa ttacgctttt gctttcgcta cagttaccat cgccgcacga
     8221 atcgtacgac cattcagcgt ataacccttc tgcataatgc ccagtacgtt acctggcgca
     8281 acgtcatcag attccaccat tgcgatagcc tgatgcacat tcgggtccag cggcacgtta
     8341 gtttcggcga tcacttcaac accaaactta cgcacaacat ccagcatcga cttcagcgtc
     8401 agctcaatgc cttcaaccat cgcagacata tccgggttag ctttatcagc cacttccagc
     8461 gcacgatcca ggctatcaat caccggcagc aattcgttga tgaatttctc cagcgcgaat
     8521 ttgtgggctt tttcaatatc cagttcagta cgacgacgca ggttttccat ttcggctttt
     8581 acgcgcaaaa taccgtcacg ttcacgggtc tgggcttcag ccagctgagc ttcgagattc
     8641 gcaatttttt catcgcgcgg atccacctgc tcagcagaag cttctggctc aactgcctca
     8701 atctcttcgt gctgatccat gataatttct tccggggctt gcccctcagg cgttttctgt
     8761 tctttactac tcatgaattt ctccgcgttt ttttcgcatt catctcgcta acttcgctta
     8821 ttatggggat cagtttcagg gtttcaaggg aagcactcac attgtcatca atcttcgcaa
     8881 caaggacctc ggaaaaatga ataatcattt caagtgtatt ggcattgtgg gacacccacg
     8941 gcaccctact gcactgacaa cacatgaaat gctctaccgc tggctgtgca caaaaggtta
     9001 cgaggtcatc gttgagcaac aaatcgctca cgaactgcaa ctgaagaatg tgaaaactgg
     9061 cacgctcgcg gagattgggc aactggctga tctcgcggta gtcgttggtg gcgacggtaa
     9121 tatgctgggc gcggcgcgca cgctcgcccg ttacgatatt aaagttattg gaatcaaccg
     9181 tggcaacctg ggtttcctga ctgaccttga ccccgataac gcccagcaac aattagccga
     9241 tgtgctggaa ggtcactaca tcagcgagaa acgttttttg ctggaagcgc aagtctgtca
     9301 gcaagattgc caaaaacgca tcagcaccgc gattaacgaa gtggtacttc atcctggcaa
     9361 agtggcgcat atgatagagt tcgaagtgta tatcgacgag atctttgcgt tttctcagcg
     9421 atctgatggg ctaattattt cgacgccaac aggctccacc gcctattccc tctctgcggg
     9481 cggtcctatt ctgaccccct ctctggatgc gattaccctg gtgcccatgt tcccgcatac
     9541 gttgtcagca cgaccactgg tcataaacag cagcagcacg atccgtctgc gtttttcgca
     9601 tcgccgtaac gacctggaaa tcagttgcga cagccagata gcactgccga ttcaggaagg
     9661 tgaagatgtc ctgattcgtc gctgtgatta ccatctgaat ctgattcatc cgaaagatta
     9721 cagttatttc aatacattaa gcaccaagct cggctggtca aaaaaattat tctaatttta
     9781 cgccagcctc tttactgtat ataaaaccag tttatactgt acacaataac agtaatggtt
     9841 tttcatacag gaaaacgact atgttggcac aactgaccat cagcaacttt gctatcgttc
     9901 gtgagcttga gattgatttt catagcggca tgaccgtaat aactggcgag accggtgcgg
     9961 gtaaatctat tgcaatagat gccctcggtc tttgtctcgg tggtcgcgct gaagccgaca
    10021 tggtgcgtac cggcgctgct cgcgctgacc tgtgcgcccg tttttctctg aaagatacgc
    10081 cagcggccct gcgctggctg gaagaaaacc agcttgaaga cgggcatgaa tgtttgcttc
    10141 gtcgcgtgat cagcagtgat ggtcgctccc gtggtttcat caacggtaca gctgttcctc
    10201 tgtcacaact gcgcgaactg ggtcagttgc tgattcagat ccatggtcag cacgctcatc
    10261 aattactcac caaacctgag caccaaaaat tcctgcttga tggctatgcc aatgaaacct
    10321 ctctacttca ggaaatgacc gcacgttatc agttgtggca tcaaagctgc cgtgacctcg
    10381 cgcatcatca acagttaagt caggaacgcg ccgcccgtgc ggaactgctg caataccaat
    10441 taaaagaact taacgaattt aatccgcagc ccggagagtt tgagcaaatc gacgaagagt
    10501 acaaacgtct ggcgaacagc ggtcaattgc tgaccaccag ccagaatgca ttggcattaa
    10561 tggccgacgg tgaagacgca aacctgcaaa gtcagcttta cacggctaaa caactggtga
    10621 gcgaattgat tggcatggac agcaaactgt ccggcgtact tgatatgctg gaagaagcta
    10681 ccatccagat tgctgaagcc agcgacgaac tgcgccacta ctgcgatcgt ctggatctcg
    10741 atcccaaccg actgtttgaa cttgaacagc gcatctcaaa acagatttcg ctggcacgta
    10801 aacatcacgt cagccctgag gcattaccac agtattacca gtcgctactg gaagaacagc
    10861 agcaactgga cgatcaggcc gactcacaag aaacgctcgc gctggcggta acgaaacatc
    10921 atcagcaggc actggaaacc gcgcgcgcat tacaccaaca acgccagcaa tatgcagaag
    10981 aacttgcaca gctgatcacc gacagtatgc atgcgctctc aatgccgcat gggcagttta
    11041 cgattgatgt caaatatgac gagcatcacc tgggcgctga cggtgccgat cgtattgagt
    11101 ttcgggtaac caccaaccca ggtcagccaa tgcagcctat tgccaaagtc gcatccggtg
    11161 gtgaattgtc ccgcatcgca ctggcaatcc aggtcatcac ggcgcgtaaa atggaaaccc
    11221 cggcactgat ttttgatgaa gtggatgtag ggattagcgg tccaacagcg gcagttgtcg
    11281 gcaaactgct gcgtcaactc ggcgaatcaa ctcaggtgat gtgtgttacc cacctgccac
    11341 aagtcgcggg atgtggtcat caacactatt ttgtcagcaa agaaaccgat ggtgcgatga
    11401 cagaaacaca tatgcaatct ctggataaaa aagcgcggtt acaagagctg gcgcgcctgc
    11461 ttggtggcag tgaagtcaca cgtaacacac tggcgaatgc gaaagaactg cttgcagcgt
    11521 aaactttttt cctgcttcac ggtcagagta aacagcaaaa cgccgtaaga ccggaaagca
    11581 aaaggtttta aagtgatgaa aggtctatta tcatcggcat attacagatg agccacgtac
    11641 tgctcgggcc cgaaaaggaa tcaaatcact atgcgctgta aaacgctgac tgctgcagca
    11701 gcagtactat tgatgttaac cgcaggctgt tccactctgg agcgagtggt ttaccgtcct
    11761 gacatcaacc aggggaacta tctgaccgct aacgacgtat ccaaaatacg tgttggcatg
    11821 acgcaacaac aagttgcgta cgcattgggt acaccgctga tgtccgatcc atttggtacg
    11881 aatacctggt tctatgtctt ccgccagcaa ccaggtcatg aaggtgtaac gcagcaaacg
    11941 ctgacgctga cctttaacag tagcggtgtg ttgaccaata ttgataacaa acctgcgctg
    12001 agtggtaact aataataaag ttgctctcaa agacgttaaa aaaggtgctc aatgagcacc
    12061 ttttttctgt ctgttattta ttcgctgatt tttctgctcg ttgcctgcga agctctttcg
    12121 gatcggcaat gagagggcga taaatctcca cccgatcgcc atcatgcaca ctatcgctta
    12181 gttttgccgg acggctgtaa atgccgactt tgtttttagt taaatcgata tcggtacgca
    12241 attccagcaa gccactggcg cgaatagctt cttcaaccgt cgcgccctcc tgcagcgtca
    12301 ctcgctgcag gtattgcttc tcaggtagcg cataagccac ctcaacggca attttaccta
    12361 gcactgtaaa cctctttcgc acgaaccgta aaagcctgga ccatattagc cgccagctct
    12421 ttaaacacgc gaccaaaggc gagttcaatc aacttattgg taaactcaaa gtcgagatga
    12481 aactcgatac gacacgcctc ctggctcagc ggcgtaaact tccatccacc aatcaatttc
    12541 ttgaacggcc catccaccag attcataaga atactttggt tactggtcaa ctggttgcgg
    12601 gtagtaaacg ttttgctgat cccagcctta gagacatcta ccgccgcagt catctgccca
    12661 ggagtggact ccagaatccg acttccggta caacctggca aaaactgagg ataagactga
    12721 acgtcattca ctaactgata catttgctcc gcgctgtagg gtaccagtgc ggtccggcta
    12781 atctgaggca taacaatttc catcaacaaa aatccaacaa ataatatcat ttatcctgct
    12841 gtaaaaaaaa cgctatcccg gcgctgggta acatcgggtt catgctaaga tagagccttg
    12901 tcccccgcag gattgatatg gggtattttc gatttcagat taccgatgat tcacgacgct
    12961 tatgacgaag aaaaaagcac ataaacctgg ttcagcgacc atcgcgctta acaagcgcgc
    13021 ccgtcacgaa tactttatcg aagaagagtt cgaagcggga cttgccctgc aaggctggga
    13081 agttaaatcc ctgcgcgcag gaaaagccaa tatcagcgac agctacgtcc ttctgcgtga
    13141 cggagaggca tttctgtttg gcgctaacat cacgccaatg gccgtggcct ccacgcatgt
    13201 ggtgtgcgat cctacccgta cccgcaagtt acttctcaac cagcgcgaac tggactcatt
    13261 gtacggtcgc gtcaatcgag aaggctatac cgtagtggcg ctctccctgt actggaaaaa
    13321 tgcctggtgc aaagtgaaaa tcggcgtcgc caaaggtaaa aaacagcacg ataaacgttc
    13381 agatatcaaa gaacgcgaat ggcaggtgga taaagcacgc atcatgaaaa acgcccaccg
    13441 ttaaacctgc actccaatta ttgaccagtt cctcaccgcg cctccctctc cggcggcgcg
    13501 aatgaacatc ttattggcta tcacatccga cacaaatgtt gccatcccat tgcttaatcg
    13561 aataaaaatc aggctacatg ggtgctaaat ctttaacgat aacgccattg aggctggtca
    13621 tggcgctcat aaatctggta tacttacctt tacacattgg ggctgattct ggattcgacg
    13681 ggatttgcga aacccaaggt gcatgccgag gggcggttgg cctcgtaaaa agccgcaaaa
    13741 aatagtcgca aacgacgaaa actacgcttt agcagcttaa taacctgctt agagccctct
    13801 ctccctagcc tccgctctta ggacggggat caagagaggt caaacccaaa agagatcgcg
    13861 tggaagccct gcctggggtt gaagcgttaa aacttaatca ggctagtttg ttagtggcgt
    13921 gtccgtccgc agctggcaag cgaatgtaaa gactgactaa gcatgtagta ccgaggatgt
    13981 aggaatttcg gacgcgggtt caactcccgc cagctccacc aaaattctcc atcggtgatt
    14041 accagagtca tccgatgaag tcctaagagc ccgcacggcg caagccctgc gggctttttt
    14101 gtgctccgaa tttgtcccgc gaagtctgat gccaactaat taaatccgat ccttttaggc
    14161 accttgttag gcacttcata aagctatatt gtctttgagg tgcctaaaac tatggaaacc
    14221 cggcaatggc aagacaaacc aaacctctat ccgttaagga aatcgaatct gctaaaccca
    14281 aggaagcgga ctacgttctc tatgatggcg atggccttga gctactcatt aaatccagcg
    14341 ggagtaaaat ctggcagttt cgctacattc gccctgtcac caagacacga gcaaagaaaa
    14401 gcataggccc ttacccgtca gttacccttg ccgatgccag aaactaccgt gcagagtccc
    14461 gctcactcct agctaaacag attgatccgc aggaacatca gcaagaacaa ttgcgtagtt
    14521 cgctggaagc caaaaccaat actttccagc tcgtagctga acgttggtgg aatgtgaaga
    14581 aagccagtgt gacagaggac tatgcggaag atatctggcg ctctcttgaa agagatgtct
    14641 ttcctgcgat tggcgacgtt agcgttacag atattaaagc tcatacactg gttcaggccg
    14701 tacaaccggt tcaggccaga ggagcactgg aaacggttcg tcgcctgtgt caacgtatta
    14761 atgaggtcat gatctatgcc cagaacacag ggctgattga tgctgttccc agcgttaata
    14821 tcggtaaagc cttcgaaaag cctcagaaaa agaacatgcc cagcattcga ccggatcagc
    14881 tatctcaact gatgcagaca atgcgaacag ccagcattag cctttccaca cgctgcctgt
    14941 tcatgtggca acttcttact attacccgcc ctgccgaagc ggctgaagct cgctgggaag
    15001 aagtagacat agaagcgcaa gagtggaaga ttcccgcagc acgcatgaaa atgaaccgcg
    15061 accatactgt tccattgtca gatgaagcaa tagctgtgct ggagatgatg aaaccgctaa
    15121 gcggcaaccg agaatttatc tttcctagcc gtatcaagcc caaccagcct atgaacagtc
    15181 agactgttaa cgcctcacta aaacgcgcag gttttggtgg tgtgcttgtt tcgcatggtc
    15241 tgagatcaat tgccagtacg gctcttaatg aacaaggctt tccgcctgat gccattgagg
    15301 cagcattggc ccatgtggat aagaatgagg ttcgtcgtgc ttataaccgc agcgattacc
    15361 tggaacagcg tcgcccgatg atgcaatggt gggcaaattt tgtgatggca gcggatcgtg
    15421 gaagcatgat tgaaggtggg ataaaaggaa tgaagctagt tgggtgacta aatagctgcg
    15481 cggcatagtg tcccactttc agccagccct tgcaggttaa gccaccagac agtaaacggc
    15541 gggtgtcgcg ttattaggcg cagctattta gtagtaatat taggattggt gattctgcac
    15601 tccagaagtt
//
LOCUS       DAZPAB010000085        15608 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZPAB010000085 DAZPAB010000000
VERSION     DAZPAB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15608
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.085"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACHUPG_002366"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="ACHUPG_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
     gene            complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACHUPG_002367"
     CDS             complement(1023..2045)
                     /gene="ytfT"
                     /locus_tag="ACHUPG_002367"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR0861960.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACHUPG_002368"
     CDS             complement(2059..3561)
                     /gene="ytfR"
                     /locus_tag="ACHUPG_002368"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR0861961.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACHUPG_002369"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="ACHUPG_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="ACHUPG_002370"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="ACHUPG_002370"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR0861962.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="ACHUPG_002371"
     CDS             complement(5877..6218)
                     /locus_tag="ACHUPG_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR0861963.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACHUPG_002372"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="ACHUPG_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR0861964.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACHUPG_002373"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="ACHUPG_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR0861965.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12574
                     /gene="msrA"
                     /locus_tag="ACHUPG_002374"
     CDS             11936..12574
                     /gene="msrA"
                     /locus_tag="ACHUPG_002374"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR0861966.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12897..14240
                     /locus_tag="ACHUPG_002375"
     CDS             12897..14240
                     /locus_tag="ACHUPG_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR0861967.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14302..14508)
                     /locus_tag="ACHUPG_002376"
     CDS             complement(14302..14508)
                     /locus_tag="ACHUPG_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR0861968.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14833..15387
                     /locus_tag="ACHUPG_002377"
     CDS             14833..15387
                     /locus_tag="ACHUPG_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR0861969.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15450..>15608)
                     /locus_tag="ACHUPG_002378"
     CDS             complement(15450..>15608)
                     /locus_tag="ACHUPG_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0861970.1"
                     /translation="AEQVDAIVSANLAADSNITKEKICEKYKSTIEAFKKEQLALPVL
                     VRCKNSET"
BASE COUNT         3676 a         4296 c         3879 g         3757 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aatcgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt
    12601 gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat
    12661 cgcccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca
    12721 gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc
    12781 tatactagcc actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc
    12841 acacaaatta atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt
    12901 taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg
    12961 agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata
    13021 taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc
    13081 aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc
    13141 cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct
    13201 ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga
    13261 aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct
    13321 tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct
    13381 tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg
    13441 cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg
    13501 aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg
    13561 tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc
    13621 cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt
    13681 ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac
    13741 aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt
    13801 ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga
    13861 tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg
    13921 aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg
    13981 acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca
    14041 tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat
    14101 tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg
    14161 tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata
    14221 aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct
    14281 tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt
    14341 aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc
    14401 cttatcaaat tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt
    14461 tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg
    14521 ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta
    14581 atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct
    14641 aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt
    14701 catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg
    14761 ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa
    14821 gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg
    14881 atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat
    14941 cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag
    15001 ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa
    15061 aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc
    15121 accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt
    15181 ctggagagta ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc
    15241 gcgcgcggtg cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac
    15301 ggtcgcgtgc aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg
    15361 gacctgctgc aaaagttgct caaataatgg caataacata aattctggaa aaataatttt
    15421 cccctatcgt tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat
    15481 actggtaacg ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa
    15541 atcttttctt tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact
    15601 tgttcggc
//
LOCUS       DAZPAB010000086        15444 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZPAB010000086 DAZPAB010000000
VERSION     DAZPAB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.086"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..74)
                     /locus_tag="ACHUPG_002379"
     CDS             complement(<1..74)
                     /locus_tag="ACHUPG_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HFR0861971.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLV"
     gene            complement(172..1281)
                     /gene="anmK"
                     /locus_tag="ACHUPG_002380"
     CDS             complement(172..1281)
                     /gene="anmK"
                     /locus_tag="ACHUPG_002380"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR0861972.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1555..2022
                     /gene="slyB"
                     /locus_tag="ACHUPG_002381"
     CDS             1555..2022
                     /gene="slyB"
                     /locus_tag="ACHUPG_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR0861973.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="ACHUPG_002382"
     CDS             complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="ACHUPG_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR0861974.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2704..2940
                     /locus_tag="ACHUPG_002383"
     CDS             2704..2940
                     /locus_tag="ACHUPG_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR0861975.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2943..3800
                     /locus_tag="ACHUPG_002384"
     CDS             2943..3800
                     /locus_tag="ACHUPG_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR0861976.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3800..5812
                     /locus_tag="ACHUPG_002385"
                     /pseudo
     CDS             3800..5812
                     /locus_tag="ACHUPG_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="ACHUPG_002386"
     CDS             complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="ACHUPG_002386"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR0861977.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6415..7311)
                     /locus_tag="ACHUPG_002387"
     CDS             complement(6415..7311)
                     /locus_tag="ACHUPG_002387"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR0861978.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7360..7599)
                     /locus_tag="ACHUPG_002388"
     CDS             complement(7360..7599)
                     /locus_tag="ACHUPG_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR0861979.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7702..8301
                     /gene="nemR"
                     /locus_tag="ACHUPG_002389"
     CDS             7702..8301
                     /gene="nemR"
                     /locus_tag="ACHUPG_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR0861980.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8338..9435
                     /gene="nemA"
                     /locus_tag="ACHUPG_002390"
     CDS             8338..9435
                     /gene="nemA"
                     /locus_tag="ACHUPG_002390"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR0861981.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9516..9923
                     /gene="gloA"
                     /locus_tag="ACHUPG_002391"
     CDS             9516..9923
                     /gene="gloA"
                     /locus_tag="ACHUPG_002391"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR0861982.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10026..10673
                     /gene="rnt"
                     /locus_tag="ACHUPG_002392"
     CDS             10026..10673
                     /gene="rnt"
                     /locus_tag="ACHUPG_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR0861983.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10766..15224
                     /locus_tag="ACHUPG_002393"
                     /pseudo
     CDS             10766..15224
                     /locus_tag="ACHUPG_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(15268..>15444)
                     /locus_tag="ACHUPG_002394"
     CDS             complement(15268..>15444)
                     /locus_tag="ACHUPG_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR0861984.1"
                     /translation="RLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGR
                     SLKQEPTEMIQATA"
BASE COUNT         3543 a         4083 c         4059 g         3759 t
ORIGIN      
        1 accagttgat taaaggcgct acaaccggag agaaggaccg gcaaaatgat aattaacagt
       61 tttttcatag tcatatcccg aagactttcc tggtccggag ggcaaatacc ctccttaatc
      121 ttccaagtgt aacggcagac acgataagaa aaaattcagt taattctgat atcacgagtt
      181 ggcggggaaa atagccccca gtaccgtctc ctggcttgcg ccagtgacgg aaggcagatt
      241 tcctggtaat cccgccagcg tccgccaggc aagccaggcg aaagccaatg cttccatgtc
      301 atcgccacta atgccaacgg catcggtggt ggtgacttct gtgcctagca gtaataccgc
      361 cagacgcgcc atgagtagcg gattacgact acctccacca cataccatca atcgttcgca
      421 gccaccgctc aacaaaactt gttcagaaat ggtcacggcg gtgagttctg ccagtgtggc
      481 ctgaacatca cggggatcaa cacccggaaa atggcgtaaa tggcgctcca gccagccgta
      541 gttaaagtat tcgcgtccgg tgcttttcgg tgcaggttgc gagaaatacg ggtcgctgag
      601 catattttgc agcagtggga gaataacttt acccgcccgt gcccactcgg catctttatc
      661 gtaaggttta cctgcctgac gccagatcca ggcatccatc agcatgttac caggaccggt
      721 atcgtagccg ccaaccagct gcccaggaat gagcagtgac agattggcga tgccgccaat
      781 attgagcacc attcgtcgct cggttgggtg agccagcaag gcatgatgga acgcaggtac
      841 cagcggtgcg ccttgtcctc ccaaggcaat atcgcgacgg cgaaaatcac cgacaaccgt
      901 aattccggtg cgtgccacaa tttgattgtt atcgccaatc tgtaaagtgt gtggtgccac
      961 gcccgtcggt tcatgccaga cggtttgacc gtgacagccg atcgcaacta tatctctcgc
     1021 ctgcaggttt tgttccttaa gcaaggcatt gaccgcatca gcaaaaagtc gcccgagttg
     1081 agtatcaagc tgtccaaact gcgaaagtgt aagctgctgg ccctggcaaa tatccagtac
     1141 agcctgtttc agagacaccg ggatcggcca gctcaagctt gccagctgtg cgacccggtg
     1201 ttcatcaatt gtcgccaaca caacatcaac accatcaagg ctggtgcctg acataacgcc
     1261 aataaagcgg cccgatttca tagttcatcc tttttcaatc tgacgtttgc gcaccactca
     1321 aacataaact tttcgtgaat accatgcgga atgaccgatt tttaccgttg gtagtaaaac
     1381 attatcttca aatcaataat catcatgaat gttttgttta taattggttg atcctacttt
     1441 cattatgatt tgctcatatt tggtagaaca tgtaaccatg gattcacata tgccatatac
     1501 tttgaccatg agggatgctt gcgtggcgtt tcatggtgaa caggagattt ttcaatgatt
     1561 aaacgcgtat tggttgtttc aatggtaggt ctgtctcttg tcggttgtgt taataacgac
     1621 accctgtcag gggatgttta taccgcttct gaagcgaaac aagtacagaa tgtcagctat
     1681 ggcaccatcg ttaacgtacg tccggtacag attcagggcg gtgatgattc caacgttatc
     1741 ggtgcaattg gcggtgctgt tcttggtggt ttcctgggga atactgttgg tggcggaacc
     1801 gggcgttctc tggctactgc agcaggcgct gttgcaggtg gcgtagctgg tcagggcgta
     1861 cagagtgcaa tgaacaaaac gcagggtgtc gagctggaaa ttcgtaaaga cgatggtaat
     1921 accatcatgg tggtacagaa acaaggcaac actcgtttct ctccgggcca acgtgtcgta
     1981 ctggccagca atggcagtca ggtgaccgtt tctccgcgct aaataagttt gcgtgtggtc
     2041 aggttactga ccacacgccc ccttcatttc accctttggc ctgtaactca atgatattat
     2101 gctcaagttt tgcgatgagc gtaatcagtt gctccagttc ctctgcggag atgccatgta
     2161 atatttccgc gcgggttttg ttaataacag cttccatttc gctgatcagt ggctctgcct
     2221 tttccgtcag tttaatacgt ttagcccgac gatcgctggc acaagtttga cgcgaaatta
     2281 accctttttc ttccagttgg tccagagtac ggaccagtga tggctgctcg atgccaatcg
     2341 cttttgccag ttgaatttgc gactggtctg gaggtaactg atggatattg tgtaacgtaa
     2401 cccaatgggt ttgtgttaac tccagcggtt tcaggcgatg gtctatcaga gcacgccata
     2461 tgcgcaccaa ccgtgccaga tcagaaccta gtggcgattc caatttcatc tccttataat
     2521 tagcttgcta agatattatg cggcttttag aatagtgtgc agcaattgta ttgctaaaac
     2581 aaatgtattg ctgcatttgg ttaccgtcag acatattttt cagaaattgc gcgtaaattt
     2641 ttcgcactta aagaatattt attaatctaa cgcaatatat tcggtcgtaa aggaatctac
     2701 tttgtgaagt ttatgctcaa tgcaacagga ttgcccttac aagatctggt gttcggtgcg
     2761 tccgtctatt ttcctccgtt tttcaaagca ttcgcgtttg gattcgtcat ctggcttgtc
     2821 gtacaccgcc tgcttcgtgg ctggatctac gccggcgata tctggcatcc cttgttaatg
     2881 gatttatcgc tgtttgcgat ttgcgtttgc cttgctctgg caatactgat tgcgtggtaa
     2941 ctatgtcaat taaaacaatt aagtatttct caacaatcat tgtagcgata gttgcggttc
     3001 ttgccggatg gtggctgtgg aattattaca tgcaatctcc gtggacacgc gacgggaaaa
     3061 tacgcgcaga acaggtttct atcaccccac aggtgtcggg acgtattgtt gagctgaata
     3121 taaaagacaa ccaactggtt aacgcggggg aacttctgct cacgatcgat aaaacgccct
     3181 ttcagatcgc cgagctgaac gcacaggctc agctggcgaa agcgcaatct gacctcgcaa
     3241 aagccaataa cgaagcgaat cgtcgtcgtc atctctcaca aaattttatt tttgccgaag
     3301 aactcgatac cgctaacctc aatgttaaag cgatgcaggc cagtgttaat gccgcacagg
     3361 cgacgctgaa acagacacaa tggcaactgg cgcaaacgga aattcgcgcc ccggtgagtg
     3421 gatgggtgac taacctcact acccgcatcg gcgattacgc tgacaccggg aaaccgctgt
     3481 ttgcccttgt cgacagccac tcgttttacg tcattggtta ttttgaagaa accaagttgc
     3541 gccatatccg cgaaggtgca ccagcacaaa ttaccctcta tagtgataac aaaacgttac
     3601 agggtcacgt ttccagtatc ggtcgggcga tttatgatca gagcgttgaa agtgactcaa
     3661 gccttattcc ggatgtgaaa cctaacgtcc cctgggttcg tctcgcccaa ctcgttcccg
     3721 tccgctttgc gctggataaa gttccaggcg atgtcacgct ggtgtcgggc accacctgta
     3781 gcattgccgt aggtcaataa tgaacgcatc gtcatggtcc ttgcgcaatt tgccctggtt
     3841 cagggccacg ctggcgcaat ggcgttatgc gttacgcaat accattgcca tgtgtctggc
     3901 gctgacggtt gcctattatt tgaatctgga tgaaccctat tgggcgatga cctcggctgc
     3961 agtggttagc tttcccaccg ttggcggtgt catcagcaaa agcctcggac gcatggctgg
     4021 cagtttactg ggagccattg cggcactgct tcttgccgga catacgctca atgagccgtg
     4081 gttttttcta ttgagcatgt cggcgtggct tggcttttgt acctaggcct gtgcgcactt
     4141 cacgaataac gtcgcgtatg catttcaact ggcgggctac acggctgcca tcatcgcctt
     4201 tccgatggtt aatattactg aggccagcca gctgtgggat atcgctcagg cgcgcgtttg
     4261 cgaggtgata gtcggtattt tgtgcggcgg catgatgatg atgatcctgc ctagcagttc
     4321 cgatgctact gcccttttaa ctgcattgaa aaacatgcac gcccgattac tggaacatgc
     4381 cagtttactc tggcagcctg aaacaaacga tgccattcgt gcagcacatg agggggtgat
     4441 tgggcagata ctgaccatga atttgctgcg tatccaggct ttctggagcc actatcgttt
     4501 tcgccagcaa aacgcgcgcc ttaatgcgct actccaccag caattacgta tgaccagtgt
     4561 catctccagc ctgcgacgta tgttgctcaa ctggccctca ccgccaggtg ccacgcgaga
     4621 aattctcgaa caattgctga cggcgctcgc cagttcgcaa acagatgctt acaccgtcgc
     4681 acgtattatc gccccgctac gcccgaccaa cgtcgccgac taccggcacg tcgccttctg
     4741 gcagcgacta cgttattttt gccgccttta tctgcaaagt agtcaggaat tacatcgtct
     4801 gcaaagcggt gtagatgatc atgccagact cgcacggaca tccgctctgg ctcgtcatac
     4861 cgacaacgcc gaagctatgt ggagcgggct gcgtacattt tgtacgttga tgatgattgg
     4921 cgcatggagt attgcatcgc aatgggatgc cggtgccaat gcattaacgc tggcagcaat
     4981 tagctgcgta ctctactccg ccgtcgcagc accgtttaag tcgttgtcac tgctgatgcg
     5041 cacgctggtg ttactttcgc tattcagctt tgtggtcaaa tttggcctga tggtccagat
     5101 tagcgatctg tggcaatttt tactgtttct ctttccactg ctggcgacaa tgcagcttct
     5161 taaattgcag atgcccaaat ttgccgcact gtgggggcaa ctgattgttt ttatgggttc
     5221 gtttatcgct gtcactaatc ccccggtgta tgattttgct gattttctta actataatct
     5281 ggcaaaaatc gttggcgttg cgctggcttg gctagcgttc gccattctgc gtccaggatc
     5341 ggatgctcgt aaaagccgcc gccatattcg cgcgctgcgc cgggattttg tcgatcagct
     5401 aagccgccat ccaacactga gtgaaagcga atttgaatcg ctcacttatc atcacgtcag
     5461 tcagttgagt aacagccagg atgcgctggc tcgccgttgg ttattacgct ggggtgtagt
     5521 gctgctgaac tgttctcatg ttgtctggca attgcgcgac tgggaatcgc gttccgatcc
     5581 gttatcgcga gtacgggata actgtatttc actgttgcgg ggagtgatga gtgagcgtgg
     5641 cgttcagcaa aaatcactgg cggccacact tgaagaatta cagcggattt gcgacagcct
     5701 tgcccgtcat catcaacctg ccgcccgtga gctggcggca attgtctggc ggctgtactg
     5761 ctcgctttcg caacttgagc aagcaccacc gcaaggtacg ctggcctcgt aattacttaa
     5821 ttacaccaca ggcatagcgt tcaccgccac cgcccagcgg tttaggttga tcggacatat
     5881 tatcgccgcc aacgtggacc atcagcgcct tgtctttgat ttcatccagt gatttcagac
     5941 gaggcgcgat gacggcatcg gtagctttgc cgtcattatt gacgaccagt gcaggcagat
     6001 cgcctaaatg cccggtacct tctggccctt catgtttacc ggtattttgt ggatcaagat
     6061 gcccgcctgc ggattccgcg gcgctggctt tgccatcttt ggtggctggc tggcagcttc
     6121 ctttggcatg aatatggaag ccatgttcac cggggggtaa tgctttcaga tcgggtgaaa
     6181 actccagacc tttatcggtt tcagtaatgg tgacgctacc aattgactgc ccaacccctt
     6241 gcgacgtgac gaggttcatc tcgacttttt cactggcagc ttgtgcgccg gtcgcaacaa
     6301 ccagcgccag aatggccaga ctaaaacgtt tcataggacc tccgttcatt gtgcattcct
     6361 gaataagtgt aaaccagtga cacatttttg aacggcggct attcctaaaa gtgcttacgg
     6421 tacgtcgtac cccagtgcag ctttacggat acgaaaccat tgttgacggg tcattttcag
     6481 tgtttctgct tcgacagctg cccgtacgcg ctcaatttta cctgagccga taattggcag
     6541 cggctgcgac ggtaaacgta atacccaggc gtaaaccacc tgttcaatcg agcccgcgtt
     6601 taactcctcc gccaccacag ccagttcatc acgcagcggc tggaaataat catcattaaa
     6661 cagacgacca ccaccaaggc aggaccacgc catcggacga acacgcagtt gttgtagttg
     6721 gtcgagcgtg ccatccagca gtaacggctg atgcaccggg gatatttcca cctgattagt
     6781 ggcaagggta aacggcagac gtgattgcaa cagggcaaat tgcgcaggcg taaagttcga
     6841 tacgccaaaa tgacgcactt tgccgctctg atgcagatgt ttgaacgcgt ccgccacttc
     6901 atcggcatcc attaacgggt ctggtcggtg gattaacagc agatccagat gatcggtcgc
     6961 gagattaatt agcgactgtt cggcgctctt aatgatgtga ttgcggtcag tgatgtaatg
     7021 accaatgacg ttttcttcac gcgcggtcgt cgcgataccg catttactga cgatttccat
     7081 ccgttcacgc aggtgaggtg ccagtttcag tgcctcgcca aacgccgctt cgcactgata
     7141 gccaccataa atatcagcat ggtccacggt ggtcacgccg agatccagat gctcttcaat
     7201 aaaactgacc agctggcggg cggacatatt ccagtccatc aatcgccagt agcccatcac
     7261 aaaacgggaa aactccgggc cttccggcgc aatagtaata cgctgaacca taatcgcttc
     7321 ctcttatcag atataagagg agtatacgca agattaggtt caaaagagtg atggttgctc
     7381 cggttcgtct gatgacgctg gcttatttgc gcgtaatttg cgcattaatc gctgccgaca
     7441 aaggcgcagc acctcttgtt tttcgccatc gctcatttta ttccagttaa aacgctcatc
     7501 ccgactacga aaacagccgc gacaaaaccc gcgttcgtca gactggcaaa ttccccggca
     7561 cgggctctgg acgggaaaga actctaattg ctccgccacc tcgccctcct cagataagat
     7621 tattaccatt attgaagctg ttaatgtcca aagtagcaac tttgcttgca ctagaccaac
     7681 tggtctacta cactccaacg catgaacaaa cacaccgaac atgatactcg cgaacatctc
     7741 ttggcgacgg gcgagcaact ttgcctgcaa cgtggattca ccgggatggg gctaagcgaa
     7801 ttactaaaaa ccgctgaagt gccgaaaggg tccttctatc actactttcg ctctaaagaa
     7861 gcgtttggcg ttgccatgct tgagcgtcat tacgccgcat atcaccagcg actggctgag
     7921 ttgctgcaat ccggcgaagg taactaccgc gaccgcatac tggcttatta ccagcaaaca
     7981 ctgaaccagt tttgccaaca tggaaccatc agtggttgcc tgacagtaaa actctctgcc
     8041 gaagtgtgcg atctgtcaga agatatgcgc agcgcgatgg ataaaggtgc tcgcggcgtg
     8101 atcgccctgc tctcgcaggc tctggaaaat ggccgtgaga accattgttt aaccttttgt
     8161 ggcgaaccgc tacaacaggc acaagtgctt tacgcactat ggcttggcgc gaatctgcag
     8221 gccaaaattt cgcgcagttt cgagccactg gaaaacgcgc tggcccatgt aaaaaacatt
     8281 attgcgacgc ctgccgttta gcaggcattt tttatcacca gacgaccggg agcctttatg
     8341 tcatctgaaa aactgtattc cccactgaaa gtgggcgcga tcacggcggc aaaccgtatt
     8401 tttatggcac cgctgacgcg tctgcgcagt attgaaccgg gtgacattcc taccccgttg
     8461 atggcggaat actatcgcca acgtgccagt gccggtttga ttattagtga agccacgcaa
     8521 atttctgccc aggcaaaagg atatgcaggt gcgcctggca tccatagtcc ggagcaaatt
     8581 gccgcatgga aaaaaatcac cgctggcgtt catgctgaaa atggtcatat ggccttgcag
     8641 ctgtggcaca ccggacgcat ttctcacgcc agcctgcaac ctggcggtca ggcaccggta
     8701 gcgccttcag cacttagcgc gggaacacgt acttctctgc gcgatgaaaa tggtcaggcg
     8761 atccgcgttg aaacatccat gccgcgtgcg cttgaactgg aagagattcc gggtatcgtc
     8821 aatgatttcc gtcaggccat tgctaacgcg cgtgaagccg cttttgatct ggtagagctc
     8881 cactctgctc acggttattt gctgcatcag ttcctttccc cttcttcaaa ccatcgtacc
     8941 gatcagtacg gcggcagcgt ggaaaatcgc gcacgtttgg tactggaagt ggtcgatgcc
     9001 gggattgaag aatggggtgc cgatcgcatt ggcattcgcg tttcgccaat cggtactttc
     9061 cagaacacag ataacggccc gaatgaagaa gccgatgcac tgtatctgat tgaacaactg
     9121 ggtaaacgcg gcattgctta tctgcatatg tcagaaccag attgggcggg gggtgaaccg
     9181 tatactgatg cgttccgcga aaaagtacgc gcccgtttcc acggtccgat tatcggcgca
     9241 ggtgcataca cagtagaaaa agctgaaacg ctgatcggca aagggttaat tgatgcggtg
     9301 gcatttggtc gtgactggat tgcgaacccg gatctggtcg cccgcttgca gctcaaagct
     9361 gagcttaacc cacagcgtgc cgaaagtttc tacggtggcg gcgcggaagg ctataccgat
     9421 tacccgacgt tgtaatccaa cattgcgagc ggcgtaaagc cgccgctata ctaaaacaac
     9481 attttgaatc tgttagccat tttgaggata aaaagatgcg tcttcttcat accatgctgc
     9541 gcgttggcga tttgcaacgc tccatcgatt tttataccaa agtgctgggc atgaaactgc
     9601 tgcgtaccag cgaaaacccg gaatacaaat actcactggc gtttgttggc tacggcccgg
     9661 aaaccgaaga agcggtgatt gaactgacct acaactgggg cgtggataaa tacgaactcg
     9721 gcactgctta tggtcacatc gcgcttagcg tagataacgc cgctgaagcg tgcgaaaaaa
     9781 tccgtcaaaa cgggggtaac gtgacccgtg aagcgggtcc ggtaaaaggc ggtactacgg
     9841 ttatcgcgtt tgtggaagat ccggacggtt acaaaattga gttaatcgaa gagaaagacg
     9901 ccggtcgcgg tctaggcaac taatctcctg ccgggcgtga actcatcgcg cccgcatctt
     9961 tactgcatcg acaagtaata tttgtcataa tgcgcgctgc aatttatccg tattaagaga
    10021 atcagatgtc cgataacgct caacttaccg gtctgtgcga ccgttttcgt ggtttttatc
    10081 ctgttgtgat cgatgttgaa acagccggat ttaacgccaa aaccgatgcg ctgcttgaga
    10141 ttgccgccat caccctgaaa atggatgaac aaggctggct gatgccggac accacattac
    10201 atttccacgt cgaaccattt gtcggcgcaa atttgcaacc agaagccctc gccttcaacg
    10261 gcattgaccc gaacgatccc gatcgcggcg cggtcagcga atacgaggcg ctgcacgaaa
    10321 tttttaaagt tgtacgtaaa ggtattaaag cgagcggctg taaccgcgcc attatggtgg
    10381 cgcacaacgc caattttgat cacagcttta tgatggccgc cgcagaacgc gcctcgctga
    10441 aacgtaaccc gttccaccct ttcgccactt ttgacactgc tgcactggcc gggctggcac
    10501 tcggacaaac cgtattgtca aaggcttgcc agaccgctgg catggacttc gacagcaccc
    10561 aggcgcactc cgcgctgtac gacaccgaac gcactgctgt gctgttttgt gaaatcgtca
    10621 accgctggaa acgtctggga ggctggccgc tacctgccgc cgaagaggtg taatcgagtc
    10681 gatgcctgat gacatgcaat gattcaggca tctatagtga ggctattcca cgcatcaagc
    10741 atgatattca cggggaatag cgttaatggc agataatcca gacccttcat cgctcctgcc
    10801 ggacgtgttt tcaccggcga cccgcgactg gtttcttcgc gcctttaaac agccgaccgc
    10861 tgtccagtcg caaacctggc atgtggcggc gcgaggcgaa catgcgctgg tgattgcacc
    10921 gactggctcc gggaaaacgc tggcagcatt tctctacgcc ctcgatcggc tcttccgcga
    10981 aggcggcgaa gatacccgcg aggcgcataa gcgtaaaacc tcacgcatcc tctatatttc
    11041 accgataaaa gccctgggca ccgacgttca gcgcaacttg cagatcccgt tgaagggtat
    11101 tgccgatgaa cggcggcggc gcggcgaaac ggtaattatt gatgaagtcc acgcggtagc
    11161 gggcagtaaa cgtggtgcgc atctggcgtt aagtctggag cggctcgatg cgctgctcca
    11221 cacctcagca cagcgaattg gcctttctgc cactgtgcgc tcagccagcg atgtggcagc
    11281 atttcttggt ggcgatcgcc cggttacggt agtcaacccg cccacaatgc gccatccgca
    11341 gatacgaatt gtcgtaccgg tcgccaatat ggatgatgtc tcatcggtcg ccagcggcac
    11401 cggcgaagac agccatgccg gccgggaagg ctccatctgg ccatatattg aaacgggtat
    11461 ccttgatgaa gtgttgcgcc atcgctcgac cattgtcttt actaattcac gcgggctggc
    11521 ggaaaaactg acggcacgat taaatgagct ttacgccgca cgcttacagc gttccccgtc
    11581 tatcgccgtt gatgcggccc atttcgagtc gacctccggc gcaacctcta accgtgtaca
    11641 aagtagcgac gtttttattg cccgctcaca ccacggctcc gtctctaaag aacaacgagc
    11701 aatcaccgaa caggcgctga aatcgggtga attacgctgc gtggtcgcaa cctccagtct
    11761 tgaactgggg attgatatgg gcgcggtgga tctggtgatt caggtggcaa cgccgctttc
    11821 tgttgccagt gggttacaac gcattggtcg cgccggacat caggttggcg gtgtatctaa
    11881 agggctgttt ttcccccgta cccggcgtga tttagtcgat tccgcagtca ttgtagagtg
    11941 tatgttcgca ggcaggctgg aaaacctgac accaccgcat aatcctctcg acgtccttgc
    12001 gcagcaaacc gttgccgccg cggcgatgga tgcattacag gtagacgaat ggtactcccg
    12061 cgtacgccgt accgcaccgt ggaaagatct gccaagacgt gtttttgacg ccacgctgga
    12121 tatgctttcc gggcgctatc cctctggcga tttttctgct tttcgcccca aactggtctg
    12181 gaacagggag accgggatat tgaccgcccg acctggcgct caattgttgg cggttaccag
    12241 cggcggcacc attccggatc gtggcatgta tagcgtgtta ttacccgaag gtgaagaaaa
    12301 ggccggttcg cggcgggtgg gtgaactgga tgaggagatg gtatatgagt cgcgggtgaa
    12361 cgacattatc actctcggcg ctacctcatg gcggatccag caaatcaccc gcgatcaggt
    12421 gattgtgact cctgctccgg gtcgttctgc ccagctcccc ttctggcgtg gtgaaggtaa
    12481 cggacgtccg gctgaattag gcgagatgat cggcgatttt cttcatttac tggcggatgg
    12541 cgcgttcttt tccgggacta ttcccccgtg gctggcagaa gaaaatacga acgccaatat
    12601 tcaggggttg attgacgagc aacgcaacgc gacgggcatc gttccgggga gtcgccatct
    12661 ggtcctcgaa cggtgccgtg atgaaattgg cgactggcgt attattttgc actctcccta
    12721 tggaagacgg gtgcatgaac cctgggcgct ggcgattgcc gggcgaatac atgccctatg
    12781 gggcgctgac gcgtctgtgg tcgccagtga tgacagcatt gttgcgcgca ttcctgacac
    12841 tgatggtaaa ttgcccgacg ccgcgatttt tttgttttaa ccagaaaagt tgctgcaaat
    12901 tgtccgcgag gcggtaggca gctcggcact tttcgccgcc cgttttcgcg aatgcgccgc
    12961 gcgggcatta ttaatgccgg gccgcacgcc gggccatcgc accccgcttt ggcaacaacg
    13021 gctgcgcgcc agtcagttgc tggaaatcgc tcagggatat ccggattttc cggtcattct
    13081 cgaaacccta cgcgaatgtc tgcaagatgt ttatgatctt cccgcactgg aacgtttgat
    13141 gcgtcgcctg aacggtggcg aaattcaaat atccgatgta acgaccacta cgccctcgcc
    13201 tttcgccaca agtttattgt tcggctatgt cgcggaattt atgtaccaga gcgacgcccc
    13261 gctggcagag cgccgggcat ccgtactgtc gctggacagc gagttactgc gcaatctact
    13321 cggacaggtc gatccggggg aattactcga cccgcaggtc attcgccagg tggaagaaga
    13381 gttgcaacga ctggctcctg gtagaagagc gaaaggtgaa gaaggattgt tcgacctgct
    13441 gcgcgaactg gggccaatga ccgttgaaga cctggcgcaa cggcatacag gcagcagtga
    13501 agaggttgcg tcgtatctgg aaaatcttct tgcagtaaaa cgaatcttcc cagcgatgat
    13561 tagcggacag gagcgtcttg cctgtatgga tgatgccgcc aggctgcgtg atgccctcgg
    13621 cgtacggcta ccagagtcat tgccagagat ttatttacat agagtcagtt acccgcttcg
    13681 cgacctcttt ctgcgctatc tccgggctca tgctctggtc acggctgaac aactggctca
    13741 tgagtttagt ctcggtattg ccattgtcga agagcagctt cagcaactgc gtgaacaggg
    13801 tctggtgatg aatctgcaac aagacatctg ggtgagcgat gaagtatttc gtcgtctgcg
    13861 tttgcgctcg ctgcaagccg ccagagaagc gacgcgtccc gttgcagcca cgacctatgc
    13921 gcgattgctg ctggaacgtc agggcgtatt acccgccacc gatggtagcc cggcgctctt
    13981 tgcctcaaca tctccaggcg tttatgaggg cgtagatgga gtgatgcggg tgatcgaaca
    14041 gcttgccgga gtcggtttac ccgcctcact ctgggaaagc cagatcctgc ctgcccgcgt
    14101 acgcgactat tcgccagaaa tgctcgatga attactggca accggagcgg ttatctggtc
    14161 gggacaaaaa aaagctgggt gaagatgacg gcctggtggc actgcatcta cagaaatatg
    14221 ccgcagaatc gttcactccc gccgaagcgg atcaggcgaa tcgttcggcg ctgcagcaag
    14281 cgataatcgc tgttctggct gacggaggag cctggtttgc acaacaaatc agccaacgga
    14341 tacgcgataa aatcgtcgaa tcggttgatt cctcggcact gcaagaggcg ttatgggcgc
    14401 tggtctggca aggcgtcatt accagcgata tttgggcacc gttacgcgcc ctcacccgca
    14461 gcagttccaa cgcacgcacc tcaactcgcc gcagtcaccg ggctcgtcgt gggcgtcctg
    14521 tctatgcaca acccgtctcg ccgcgggtat cttacaacac accaaatctg gctggacgct
    14581 ggtcgttatt gcaggtggag ccactaaacg ataccgaaag gatgctggcg ctggcggaaa
    14641 atatgctcga ccgctacggc atcatcagtc gtcaggcggt gatagccgaa aatatccctg
    14701 gcgggtttcc atcgatgcaa acactttgtc gaagtatgga agactccggg cgaattatgc
    14761 gaggtcgttt tgtagaaggt ctgggtggcg cgcaattcgc tgaacgtctg actattgacc
    14821 gattgcgcga tctggcgaca caagccacgc aaacacgcca ctatacacca gtggcgctct
    14881 ccgccaacga tccggctaat gtgtggggaa atcttctgcc ctgggctgca catccggcaa
    14941 cgctggtacc aacgcgtcgg gcgggtgcgc tggtggtcgt ttctggcggc aaattgttac
    15001 tctatctggc gcaaggcggc aaaaaaatgc tggtctggca ggaaaaggag gaattactcg
    15061 ccccagagat tttccaggcg ctgaccaccg cactgcgtcg cgaaccaagg ctgcgcttta
    15121 cgctaacaga agtgaatgat cttccggtcc ggcaaacgcc gatgtttacg ctgctgcgtg
    15181 aggcgggatt ttcaagttcg ccacaagggc tggattgggg atagttgaca tcctccacgc
    15241 cctgaaagac gtggttccct gctacgttca ggctgtcgcc tgaatcattt cggtgggttc
    15301 ctgcttcaac gagcggcctg actgcaccat ctctccacag gcaagaacgg cgtgccccgc
    15361 cgctaaaatg ttacgagcgc catttacatc ggcgttcgct gtatatccac atacctggca
    15421 tctgaattta ctttgtgaca ggcg
//
LOCUS       DAZPAB010000087        15152 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZPAB010000087 DAZPAB010000000
VERSION     DAZPAB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.087"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..738
                     /locus_tag="ACHUPG_002395"
     CDS             <1..738
                     /locus_tag="ACHUPG_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFR0861985.1"
                     /translation="KDAAEEAKSPVIISFSTGFVGNTSFEDFSHMMVSMAQKATVPVI
                     THWDHGRSIEIIHNAWTHGMNSLMRDASAFDFEENIRLTKEAVDFFHPLGIPVEAELG
                     HVGNETVYEEALAGYHYTDPDQAAEFVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVK
                     RVRDAVSVPLVLHGASGISDADIKTAISLGIAKINIHTELCQAAMVAVKENQDQPFLH
                     LEREVRKAVKERALEKIELFGSDGKAE"
     gene            743..1690
                     /locus_tag="ACHUPG_002396"
     CDS             743..1690
                     /locus_tag="ACHUPG_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR0861986.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            1700..2680
                     /gene="ydjG"
                     /locus_tag="ACHUPG_002397"
     CDS             1700..2680
                     /gene="ydjG"
                     /locus_tag="ACHUPG_002397"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFR0861987.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            2817..3575
                     /locus_tag="ACHUPG_002398"
     CDS             2817..3575
                     /locus_tag="ACHUPG_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR0861988.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            3693..5051
                     /locus_tag="ACHUPG_002399"
     CDS             3693..5051
                     /locus_tag="ACHUPG_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0861989.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(5144..5785)
                     /gene="pncA"
                     /locus_tag="ACHUPG_002400"
     CDS             complement(5144..5785)
                     /gene="pncA"
                     /locus_tag="ACHUPG_002400"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR0861990.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(5796..6812)
                     /gene="ansA"
                     /locus_tag="ACHUPG_002401"
     CDS             complement(5796..6812)
                     /gene="ansA"
                     /locus_tag="ACHUPG_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR0861991.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(6979..8835)
                     /gene="sppA"
                     /locus_tag="ACHUPG_002402"
     CDS             complement(6979..8835)
                     /gene="sppA"
                     /locus_tag="ACHUPG_002402"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR0861992.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            8996..9547
                     /locus_tag="ACHUPG_002403"
     CDS             8996..9547
                     /locus_tag="ACHUPG_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR0861993.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            9664..10707
                     /gene="selD"
                     /locus_tag="ACHUPG_002404"
     CDS             9664..10707
                     /gene="selD"
                     /locus_tag="ACHUPG_002404"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR0861994.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            10712..12673
                     /gene="topB"
                     /locus_tag="ACHUPG_002405"
     CDS             10712..12673
                     /gene="topB"
                     /locus_tag="ACHUPG_002405"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR0861995.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            12800..13840
                     /locus_tag="ACHUPG_002406"
     CDS             12800..13840
                     /locus_tag="ACHUPG_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR0861996.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(13957..15150)
                     /gene="gdhA"
                     /locus_tag="ACHUPG_002407"
     CDS             complement(13957..15150)
                     /gene="gdhA"
                     /locus_tag="ACHUPG_002407"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005094017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR0861997.1"
                     /translation="MERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGG
                     MRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMT
                     ELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEA
                     TGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTV
                     VDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQNE
                     LDVDAAHQLIANGVKAVAEGAYMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEM
                     AQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAML
                     AQGVI"
BASE COUNT         3842 a         3549 c         3943 g         3818 t
ORIGIN      
        1 aaagacgcag ccgaagaagc gaaatccccg gttattattt cttttagtac aggttttgtc
       61 ggtaacacct catttgaaga tttctctcac atgatggtat caatggcaca aaaagcaacg
      121 gtgccggtaa taactcattg ggatcatggt cggagtattg agattattca taacgcctgg
      181 actcatggta tgaattcatt aatgcgtgat gcttctgcat ttgatttcga agaaaatatt
      241 cgtttaacca aagaggctgt tgatttcttt catccgctgg gtattccggt agaggcggaa
      301 ttagggcatg tcggtaatga aaccgtttat gaggaagcgc tggcgggcta tcactatacc
      361 gatcctgacc aggcggctga atttgttgaa cgtacgggct gtgactcatt ggctgtcgcc
      421 atcggcaacc agcatggggt ttatacgtca gagccgcaat tgaactttga ggtcgtcaaa
      481 cgcgtacgcg atgctgtttc tgttccgctg gttttgcatg gcgcatcggg gatcagtgat
      541 gccgacatta aaactgcaat ttcgttgggt attgcgaaaa tcaacattca tacggagctc
      601 tgtcaggccg caatggtagc agtgaaagaa aaccaggatc agcctttcct gcatttagaa
      661 cgtgaagtac gtaaagctgt aaaagaacgg gcattggaaa aaattgaact gttcggttct
      721 gatggcaaag cggaataatg atatggataa tctcgacgtt atttgtatag gtgccgctat
      781 tgttgatatt ccattacaac cggtcagtaa aaatatcttt gatgtagatt cttatcctct
      841 tgaaagaatc gcaatgagca ccgggggcga tgcaataaat gaagcaacaa ttatttctcg
      901 tctggggcat cgtaccgcat taatgagccg cattggtaaa gatgcctcag ggcaatttat
      961 tctcgaccat tgtcgcaaag agaatattga tattcaaagc ctgaaacagg acgtcagcat
     1021 agatacatct attaacgttg gactggtgac ggaggatggc gagcggacgt ttgtcacgaa
     1081 ccgtaacggc agtttgtgga aattaaatat tgacgatgtt gattttgcgc ggtttcctca
     1141 ggcgaaatta ttatcactgg ccagtatttt caacagtcct ctattggatg gtaaagcgct
     1201 aacagaaatt tttacgcaag cgaaagcccg gcagatgatc atttgtgccg atatgatcaa
     1261 accgcggttg aatgaaacgc tggatgatat ttgcgaagca ttgagttatg tcgattatct
     1321 gtttccaaat tttgccgagg aaaaattact cacagggaaa gagacactgg atgaaattgc
     1381 tgactgcttt cttgcgtgcg gcgtaaaaac ggtggtgatt aaaacgggta aagacggctg
     1441 ctttatcaag cgtggtgaca tgacgatgaa ggtgccggcg gtcgcaggaa taaccgccat
     1501 cgacaccatt ggcgcgggcg ataactttgc ttcaggtttt attgcggcac tgttagaagg
     1561 caaaaatctg cgtgaatgcg cacgctttgc caatgcaacg gcggctatct cggttctaag
     1621 cgtcggtgcc accaccggcg taaaaaacag aaagctggtc gaacaattgc tggaagaata
     1681 cgaaggataa tgaaggcaaa tgaaaaagat acctttaggc acgacggata taacgctttc
     1741 gcgaatgggg ttggggacat gggccattgg cggcggtcct gcatggaatg gcgatctcga
     1801 tcggcaaata tgtattgata cgattcttga agcccatcgt tgtggcatta atctgattga
     1861 tactgcgcta ggatataact ttggcaatag tgaagttatc gtcggtcagg cgttaaaaaa
     1921 actgccccgt gaacaggttg tagtagaaac caaatgcggc attgtctggg aacgaaaagg
     1981 aagtttattc aacaaagttg gcgatcggca gttgtataaa aacctttccc cggaatctat
     2041 ccgcgaagag gtagaagcca gcttgcaacg tctgggtatt gattacatcg atatctatat
     2101 gacgcactgg cagtcggtgc cgccattttt tacgccgata gctgaaactg tcgcagtgct
     2161 taatgagtta aaggctgaag ggaaaattcg cgctataggc gctgctaacg tcgatgctga
     2221 ccatatccgc gagtatctgc aatatggtga actggatatt attcaggcga aatacagtat
     2281 tctcgaccgg gcaatggaaa acgaactgct gccgctatgt cgtgataatg gcattgtggt
     2341 tcaggtttat tccccgctag agcagggatt gttgaccggc actatcactc gtgattacgt
     2401 tccgggcggc gctcgggcaa ataaagtctg gttccagcgt gaaaacatgc tgaaagtgat
     2461 tgatatgctt gaacagtggc agccactttg tgctcgttat cagtgcacaa ttcccactct
     2521 ggcactggcg tggatattaa aacagagtga tttaatctcc attcttagtg gggctactgc
     2581 accggaacag gtacgcgaaa atgtcgcggc actgaatatc aacttatcgg atgcagacgc
     2641 aacattgatg agggaaatgg cagaggccct ggagcgttaa atactcaaca cgattcacct
     2701 ctgtttctgt gaaaactgtg atgaaaatct gtgattcaaa taggttattt tgaaagtaaa
     2761 catcggttat gcgataatcg cgctaatgtg atgtaaaaag atacaggggt gtaatcgtgg
     2821 cggcaaaaga caggattcag gcaattaagc aaatggttgc caacgataaa aaggtgacag
     2881 tctcaaattt gagtgggatt tttcaggtaa ccgaagaaac cattcgccgc gatcttgaga
     2941 agctggaaga tgaaggcttt ttgaccagaa cctatggtgg tgctgtttta aatacagcgg
     3001 tgttgacgga gaatatccat ttttataagc gcgcttcatc gttttatgaa gagaagctgc
     3061 ttattgcacg caaggcacta ccctttatcg acaataaaac caccatggca gccgattcca
     3121 gtagtaccgt aatggaattg ctcaaattgt tacaggaccg tagtgacctg acgttgctaa
     3181 ccaactcggc ggaagcgatt catgttctgg ctcagtcaga aattaaagtc gtttcaacgg
     3241 gtggggaact aaacaaaaat acactttccc tgcaaggaag aattactaaa gagatcatca
     3301 gccgctacca tgtcgatatc atggtaatga gttgtaaagg tcttgatatt aacagcggcg
     3361 cgctggactc taatgaagcg gaagctgaaa tcaaaaagac aatgatccgt caggcgacag
     3421 aagttgcgtt attggttgat cactctaagt ttgatcgtaa agcttttgtc cagttagctg
     3481 actttagtca tattaattat ataataactg ataaatcacc gggtgcagag tggatagcat
     3541 tttgcaaaga caataatatc cagctggtgt ggtaatttaa gcacacgttg tcaatattat
     3601 ttgttcctgc gactggaaat aaaatgatga taaagatgcg gcaagtgaat ttgccgcaat
     3661 tgctaccttt tatagcaaat ccgaggcacc caatggaaca atatgatcaa attggcgcaa
     3721 gactggaccg cttgcctttg gcccggtttc attatcgtat atttggtata ataagcttta
     3781 gtctgttatt aacggggttt ttgagttact caggtaatgt cgtcttagca aagctggtaa
     3841 gcaatggatg gtcaaataat ttcctctatg ccgcctttac ctcggcatta atgtttggtt
     3901 atttcatcgg ctcacttact ggtgggttta ttggtgacta ctttgggcgg cgcagggcgt
     3961 ttcgcataaa tcttctcatc gtcggtattg ctgcaacagg ggccgctttt gtccctgata
     4021 tgtactggct cattttcttt cgcttcctga tgggaacagg aatgggggcg ctgattatgg
     4081 ttggctatgc ctcatttacg gagtttatcc ccgcgacggt gcgtggaaaa tggtccgcgc
     4141 ggctctcatt tgttggtaac tggtcgccca tgctgtctgc ggcgataggc gtggtggtta
     4201 tcgctttttt tagttggcga ataatgtttc ttctgggtgg tattggcata ctgttagcct
     4261 ggtttctctc aggtaaatac tttatcgagt cgccacgatg gctggaaggg aaagggcaaa
     4321 tcgccggtgc agaaagccaa cttcgtgaag tagagcagca aattgaaagg gagaagagta
     4381 ttcgtttacc ccagcttact ttgaaccaga gcaacagcaa ggttaaggta atcaagggta
     4441 ccttcaggct cctgtttaaa ggggaaatgt tacgacgtac attagttgcg ataactgttt
     4501 taattgcaat gaacatttcg ctttatacca ttaccgtatg gataccgacc atatttgtta
     4561 actccggcat tgatgtcgat aaatcaatat taatgaccgc tgttattatg attggtgctc
     4621 cggtaggaat atttattgcg gcattaatta ttgatcattt tcctcgtcga ttatttggct
     4681 ccgccttact tattattatt gccgtgttag gctatatcta ttcaagtcag actacagagt
     4741 gggcgatttt aatctatggt ctggtgatga tcttcttttt atacatgtat gtttgcttcg
     4801 cgtcggcggt ttatatcccg gagctttggc caacgcattt acgcctgcgc ggttcgggtt
     4861 tcgttaatgc cgtcggacgg atcgtcgcag ttttcacgcc ctatggcgtt gcggcattat
     4921 taacacatta tgggtcgatc acggtgttta tggtgcttgg tgtcatgtta gtgctctgtg
     4981 cgctggttct ctccattttt ggcatcgaaa cgcggaaggt gtcgctggaa gagatttctg
     5041 aggtgaatta aatctttcgt tgatggcatc aattgccaga tgtaccactg acgtgtttta
     5101 tttggcctac aatgccagta cagaattgta ggccagcgta aaattacccc tgcgtctctt
     5161 cccagtctgc cagcgtatat agcgttgccc cagctgctga catctccata aacgcgtgtg
     5221 cactgtcctg gggctggata ttcacgccac gacagccatc ggtaatcacg tttaccttat
     5281 aacctaactg taacgcgtcc agcacggtaa acttcacgca atagtcagta gccaggccca
     5341 taacgatcaa ttcatcgatt tcatgatcgc gcaaccagtc atcaagcgcg gttttctgcc
     5401 gacgtccgtt atcaaaaaag gcactgtaac tgtcaactaa aggattttcg cctttatgga
     5461 acaccgctgc gatctctttt tgattcagta acggatgtaa ttgtgcgcct tcactgttct
     5521 gcacacagtg ttcaggccag aaagtttgcc gcaggccgcc gagttggcct ggcgtataag
     5581 gctctacgcc gtgctggctg gcaaagctgc cgtgattcgc cggatgccag tcctggctgg
     5641 cgataaccgc ttctccgcgc gactggcacc agtcaataag gcggttagca acctccaccg
     5701 tactgtcacc ttccggtacg gcgagcgcgc caccagcaca gaaatcattt tgtaaatcga
     5761 ccagtaacag ggcgcgatgg ggcattgcag tctccttaat catccggcgt cagttcgccg
     5821 cgcaggtttt ggctcatggc cttgcgaatg gtttcagtat ccagttcctg gctcagcagg
     5881 taatgcagtt tggttagcgt ggcttctaca gtcatatctg caccgtcaat aacgccggca
     5941 tgggcgaggg cgttaccggt ggcataacca ctcatattca ctttaccgga catacattgt
     6001 gtcaggttga ccaccacaat accgcgatcg ctggcttctt gtaattcctg caggaaggct
     6061 ttgttttgtg gcgcattacc cacgccatag gagcgcagaa tcaatgcttt caccggttgg
     6121 cgcagaaaat tgcgcacgac gtcagcagaa atccctggat aaatcgtcac cacgccaatt
     6181 ggttgtggcg tgattggatg aacgattaat tccccttcac cgtgcggggc gggtggcgta
     6241 ttcaaacgac gaatatggat accagcttcc agtaacggag gaaggtttgg agaggcaaac
     6301 gcatcaaaac catcggcatg ggctttggta gtgcggttgc cgcgatacaa tcggttattg
     6361 aaaaagagcg ttacttcgtt gatcggataa ttcggcgcaa cgtacaacgc attcagcaga
     6421 ttaatttgtc cgtcagagcg taactcagcc agcgggattt gtgaccctgt cacaatgacc
     6481 ggtttaccga gattctcgag catgaacgac agcgcagagg cggtatacgc catcgtgtcg
     6541 gtaccgtgta gaatgacaaa accatcatag tcgtcatagt gcgctttaat atcttcagca
     6601 atatgctgcc agtcttccgg cgtcatatca gaagaatcca tcaacggcgt atattcatga
     6661 atggtgaaat ctggcatctc cgggcgatgg aattccggca tcagcgccag ttggcgttgt
     6721 agatgacctg acaccggaat ataaccctgc tcggaacgct gcatcccgat ggtcccgccc
     6781 gtgtaggcga cgtaaattga tttcttttgc atgatattga tatgaatgat atcgaaagag
     6841 caaaagtata tacgtgaggc aggaggagcg cacctgatac tatgcggata aggaaatatg
     6901 gggcgagaca gcgggaagag gattcgtcga ggtaaaaaat aatgtggccg acgatctgtc
     6961 ggccactcag tacaagactt aacgcacgtt ggcgcaggtc aggcaaaacg cataacggtt
     7021 ttgtgggtcg ttaaacgcgg ccagcttgtc gctttcactt ttaacggtag aggctaccga
     7081 ggcaagcggt gcaggtaaca tggcctggaa cgcatctggc aacattgccc ggacagaacc
     7141 agacatgttg tccatcactt tgtcgaagaa ggtcggttca tcaacgtagt attccagatg
     7201 ccactgtttc acttttgcga gctctgctgc tttggcaacc gcatcatcga aatccccgag
     7261 actatcgacc agcccgttag cttttgcatc ctgaccggtc cagacgtggc cctgggcgat
     7321 tttatcaatt tgctccggcg tcgaatgacg cgcatcagca accagcgtga taaagcgttt
     7381 atagccattc tcaatgctta attgcatcat ctgctgcgct tccggcggca gtgccctggt
     7441 gatagaaaca tccgccagcg gtgaagttga gacaccatca gtatgaacac caatcgaatc
     7501 cagactattt tctacggtgg tgatcacgcc gaagataccg atagaaccgg tcagggtgct
     7561 ggggttagcc acaatgtaat tagctggcgt ggaaatccag taaccaccag atgccgccat
     7621 gccgcccatc gatacaacca cagacttacc cgctgcccgg gctgctgcca gttcagcgcg
     7681 aatcacttca gacgcggtaa cgctgccgcc tgggctatta acacgcagga caatcgcttt
     7741 cactttcggg tcaaggcgag cgtcgcggat ttgtgccgca gtggtatcac cgccaacatt
     7801 cccctgagtt tcctcgccat ccataattgc gccattagcg aagacgacac cgatgctgtc
     7861 accggtatct gccggcgttt tcaatgcgta atcgtaataa ctgatggcgc gataattttt
     7921 atcagtctta ctccagccga actctttggt cagagctttt tcgatttcgg cactcgatgc
     7981 cagtgcatcg accagcttgt tttccagtgc atatttcgcg gtatcgccac cggttttggt
     8041 taaaccctca agcaaccctt gcgcgccagg gaatacctgc tgagcaggga tctgccggtt
     8101 agcggcaaca gtattcagat agttttgcca cagctcacca atccagcggc tgtcagcttc
     8161 gcgggctgcc ggtgacatat catcacgaat aaacggttca acggcagatt tatacgtacc
     8221 cacgcggaac acatgggtgg aaactttcag cttatccagc aacgatttgt agtacagacc
     8281 gttggtagca aaaccgtgca gatcaaccac gccttgcggt gacagccaaa ttttattggc
     8341 gaaactggcg agataatatt gcccctggct gtagttctcg ccaacggcat aaaccggttt
     8401 cccgctgtca cgaaactctt tcagggcttt gccgatgtac tgcatagacg gctggtcgcc
     8461 gcctgcgaag tttttcagat ccatcacgat accggtgata ttgcggtcgt ccttcgcctg
     8521 gcgaatggtg ttgacgatat caaacagtga gttttcctgc agacgatcgg aactggcacc
     8581 aagcagctgg cggcttaatt tactaaaccg ctgagaactg tctggtttat cgacgatcac
     8641 accagaaatg tccagcagca gtgcgccacg actggccgtt tctttcgaat caccaccact
     8701 gacctgcatc cagatcccca caccaaccag tacgaggaaa ataaagaaca ggttaagtac
     8761 catttcacgg acgaaattca gcagacgcca cgtccattta aaaaatccgg caataaatcg
     8821 ccaaagggtt cgcatgtatt ctcccaactt aaggtcacac ctgtcacagg cgcaatagat
     8881 gtggttatcg taatgaccca accggcactt gtcagcagga attgcggact gtgctgtaac
     8941 aaaatccact ctcgtgttaa ttttgtgaat aaatatcacg acaggagtta atcaaatgga
     9001 tgcactcgaa ctattgatca atcgccgtag cgcctcccgc ctggctgaac ccgcgccaac
     9061 gggtgaacaa ctgcaaaaca tcctgcgtgc gggtatgcgt gcgccggacc ataagtccat
     9121 gcaaccgtgg catttttttg tgattgaagg ggaagggtgc gagcgtttca gcgccgtact
     9181 ggaacagggg gcgattgctg ccggtagtga tgataaagct atcgacaaag cccgtaatgc
     9241 gccgttccgc gcaccgctca tcatcacggt ggtggcgaaa tgcaaagaaa atcataaagt
     9301 cccgcgatgg gaacaggaaa tgtctgccgg atgcgcggtc atggcgatgc aaatggcagc
     9361 cgttgcccag ggatttggcg gcatctggcg cagtggcgca ttaactgaaa gtccggtagt
     9421 gcgtgaagca ttcggttgcc gtgagcagga taaaattgtc ggttttctct acctcggcac
     9481 gccgcagttg aaagcatcta cgtcgattaa cgtcccggac ccgacgccgt ttgtaactta
     9541 tttctgataa ttgtcgcgaa actgtctgga ttgtgagcaa aggcgcagga attcagacac
     9601 tctcacttat cacttcacgg aatgagggtt accatacccg gattgcattg acaggagatg
     9661 tccatgagcg agaactcgat tcgtttgacc caatacagcc acggagctgg ttgcggctgt
     9721 aaaatttccc caaaagtgtt ggaaaccatc ctgcacagtg agcaggcgaa gtttgttgat
     9781 ccgaatttgc ttgtgggtaa tgaaacccgc gacgatgcgg cggtgtacga tctgggcaat
     9841 ggcaccagcg ttatcagtac caccgacttc tttatgccga tcgttgataa tcctttcgat
     9901 tttggccgca ttgcggcgac taacgccatc agcgatatct tcgcgatggg gggcaaaccg
     9961 attatggcga ttgcgatcct cggctggccg attaacaaac tttccccgga aattgcccgc
    10021 gaagtaaccg aaggtggacg ctatgcatgc cgtcaggcgg gtattgcgct ggctggcggt
    10081 cactccatcg atgcgccgga gccgattttt ggtctggcgg taacggggat cgtaccgacc
    10141 gagcgggtga agaaaaacag taccgcacaa gccggatgca aactgttcct gacgaaaccg
    10201 ctggggatcg gcgttcttac cacggctgag aaaaaatcac tgttgaaacc agaacatcag
    10261 ggactggcga cggaagtgat gtgccggatg aacatcgcag gcgcgtcctt tgccaacatc
    10321 gaaggcgtaa aagcgatgac cgacgttacg ggctttggtc tgctgggcca cttgagcgaa
    10381 atgtgtcagg gggctggtgt gcaggcacgc gtcgactatg aagcgatccc gaaactcccc
    10441 ggtgttgaag agtacattaa gttgggcgca gtacctggcg gcactgaacg taactttgcc
    10501 agctacggtc atctgatggg tgaaatgccg cgtgaagtgc gcgatctgct gtgcgatccg
    10561 caaacttctg gcggtttgct gctggcggtc atgccggaag cagaaaatga ggttaaaact
    10621 acagccgccg agtttggcat tgaactgacg gcgattggcg aactggtgcc agcgcgcggc
    10681 ggtcgtgcca tggttgagat ccgttaattt aatgcggttg tttattgccg aaaaaccgag
    10741 tctggcgcgc gccattgctg atgtcctgcc caaaccgcac cggaaaggcg atggctttat
    10801 cgagtgcggt aatggtcagg tggtgacttg gtgtatcggt cacctgcttg agcaggcgca
    10861 gccagacgcc tacgacagcc gctatgcgcg ctggaatctt gcggatttgc cgattgtccc
    10921 ggaaaagtgg caattacagc cacgaccctc cgtgaccaaa caacttaacg tcatcaaacg
    10981 cttcctgcat gaagccagcg aaatcgttca cgccggggac ccggatcgtg aagggcaatt
    11041 gctggtggat gaagtgctgg actatctgca actggcaccg gaaaagcgcc agcaggtgca
    11101 gcgttgcttg ataaacgacc tgaacccgca ggcggttgag cgggcgatcg accgtcttcg
    11161 ttccaacagt gagtttgtgc cattgtgcgt ttctgcgctg gcgcgagcgc gtgccgactg
    11221 gctgtacggc atcaatatga cccgtgcgta taccattctc ggtcgcaatg ccggttatca
    11281 gggcgtactt tccgtgggac gcgtgcagac acccgtgctc gggctggtgg tgcgccgcga
    11341 tgaagagatt gaaaacttcg tggcgaaaga cttctttgaa gtcaaagcac atatcgtgac
    11401 acctgccgat gagcggttta ccgctatctg gcaaccgagc gaagcgtgtg aaccgtacca
    11461 ggatgaagaa gggcgcttgt tacatcgtcc actggcggag catgtggtta accgcattag
    11521 tggtcaaccg gctattgtca ccagctataa cgataaacgg gaatcagaat ccgcgccgct
    11581 gccgttttcg ctttcggcgt tgcagattga agcggcaaaa cgcttcggtc tgagcgcgca
    11641 gaacgtgctt gatatctgcc agaagctgta cgaaacgcac aagctaatca cttatccgcg
    11701 ttccgattgt cgctatttgc cagaagaaca ttttgccgga cgccacgcgg tgatgaatgc
    11761 catcagcgtt catgcaccgg atctgttgcc gcagccagtg gtagatccag atatacgcaa
    11821 ccgctgttgg gatgacaaaa aggtcgatgc gcaccacgcc atcatcccga ccgcacggag
    11881 ttctgcgatc aacctgacgg agaacgaagc gaaggtctat aacctgattg cccgtcaata
    11941 cctgatgcag ttctgcccgg atgcggtgtt ccgcaagtgt gttatcgaac tggacattgc
    12001 caaaggcaaa tttgtcgcta aagcccgttt tcttgctgaa gcaggctggc gcacgctgtt
    12061 aggcagcaaa gagcgcgatg aagaaaacga cggcacgcca ttgcctgtgg tggcgaaagg
    12121 cgatgagttg ctgtgtgaaa aaggtgaagt ggtagagcgg caaacccagc cgccgcgcca
    12181 ttttaccgat gcaacactgc tttcggcgat gaccgggatc gcgcgctttg tgcaggacaa
    12241 agatctgaaa aaggtccttc gtgcgaccga tggtctgggg acagaagcaa cccgtgccgg
    12301 gattattgaa ctgctgttca agcgtggttt tctgaccaaa aaagggcgct atatccactc
    12361 caccgacgcc ggaaaagcgt tattccattc gctgccagaa atggcgacgc gaccggacat
    12421 gaccgcgcac tgggaatcgg tgctgacgca aatcagcgaa aagcagtgtc gctatcagga
    12481 ctttatgcag ccgctggtgg ggacgctata tcagcttatt gatcaagcca aacgtacgcc
    12541 ggtgcggcag tttcgcggca ttgtggctcc gggcagtggt ggcagtgctg ataagaaaaa
    12601 ggctgcaccg cgtaaacgta gtgcgaaaaa aagtccgcca gcagatgaag ccggaagcgg
    12661 ggcgatagcg taagcgagcg aatctttcgt gctattcgag tcatattctg aaatatccag
    12721 cggatcaaga aagttcgttg gatatttttt tgcatggata aaattatcgc ctctaaagta
    12781 tgtaataaca gggaatgtga tgaaaaaagt tttattgcaa aaccatcctg ggagcgagaa
    12841 gtattctttt aatggctggg gaatatttaa tagtaatttt gaacgggtga ttaaagaaaa
    12901 taaggccatg ctgctttgta agtgggggtt ttatttaaca tgtgttgtcg ctgtaatgtt
    12961 tgtattcgca gcgataacat ccaacggttt gaatgaaaga ggcctgatta ccgcgggatg
    13021 ctcttttctt tatctattaa ttatgatggg gcttattgtt cgggccggtt ttaaagcaaa
    13081 aaaagaacaa ctgcattatt atcaggctaa aggtattgag ccgctcagta tcgagaagtt
    13141 acaggcgcta caattgatcg caccttatcg attctatcat aagcaatggt ctgaaacgct
    13201 agagttctgg ccgcgaaagc ctgaacctgg caaagatacc ttccaatatc atgtgcttcc
    13261 ttttgactcg atcgatatca tcagtaaaag acgcgagtct ttagagaatc aatggggtat
    13321 cgaagatagc gaaagttatt gtgccttaat ggagcatttt ctttctggcg accatggagc
    13381 caataccttt aaagcaaaca tggaggaagc cccagagcag gttatcgcct tgttgaataa
    13441 atttgctgtt tttccctcag actatatctc tgattgcgct aataatcgct ccggtaaatc
    13501 ctcggcgaag ctaatatggg cggcggaatt atcatggatg atctcgatat caagcacagc
    13561 ttttcaaaac gggacaattg aagaagaact ggcctggcat tatataatgc ttgcttctcg
    13621 aaaggcgcac gagttgttcg aaagcgaaga agattatcaa aaaaatagtc taatgggatt
    13681 tctttactgg catatctgct gctatcgcag aaagttaacg gatgctgaac ttgaagcgtg
    13741 ttatcgttac gacaagcagt tttgggagca ctacagtaag aaatgccgtt ggcccataag
    13801 aaatgttccc tggggagcat catccgttaa atactcataa acgcctgaaa ttttgccggg
    13861 ggcgctgcgc ttgaccggcc tacattagag cgtaactgca atgaattgtg cccatctgta
    13921 ggcctgataa gcgtagcgcc atcaggcatt tacaacttaa atcacaccct gcgccagcat
    13981 cgcatcggca accttcacaa aaccggcaat gttcgcgccc tgcacgtagt tggtttgctc
    14041 accttcacca ccatgctcaa cacaggcatg gtggatatcc agcatgatgt gatgcaaacg
    14101 tgcgtcaact ttctcggctt tccagcccag gcgcgcagcg ttttgtgcca tttccaggcc
    14161 cgacgttgct acaccgccag cattagccgc tttacccggt gcaaacagta cgcctgcctg
    14221 ctggaacagt tcagtcgctt cgatggtggt cggcatatat gccccttcag cgacggcttt
    14281 aacgccattg gcgataagct gatgcgcggc gtcaacatcc agttcgttct gggtggcgca
    14341 aggcagggcg atatcaaccg gtacagacca cggctgttgg ccttcgagat agaccagacc
    14401 aaattctttg gcgtaatctg ccgctcgacc atcgcggctg gctttgattt cgataagacg
    14461 tgccagtttc tctttcgtga atccgctttc atcaactaca gtgccgctgg agtctgacgc
    14521 agtgatcaca cgagcaccaa attccatcgc tttttcgata gcgtactggg cgacgttgcc
    14581 ggagccagaa acggaaacgc tcatcccttc aaaacccata ccgtggcgtt ttaacattgc
    14641 ttctgtgaaa taaaccagac cgtagccggt agcttccggg cgaataagac taccgccaaa
    14701 tgaaaggccc ttaccggtga agacgcaggc ggtattgttg gagagctttt tcatcatccc
    14761 cgccataaag ccgacttcac gaccaccaac gccgatatca cctgccggaa cgtcggtatc
    14821 cgcgcccagg tggcgataca gttcagtcat cagcgtctgg caaaaacgca tcactttacc
    14881 ttcgcttttt cctttcggat cgaaatcgct gccgccttta ccaccgccca tcggcagggt
    14941 agtcagggca tttttgaagg tttgttcaaa gccgaggaat ttgagaatgg aaaggttaac
    15001 tgacggatgg aagcgcatac cgcctttgta cgggccgatg gcagagctga actgcacacg
    15061 ccatgcacgg ttgacctgta tctggttgcg atcatcaacc cataccacgc gaaactggat
    15121 cacgcgctcc ggttcaacca gacgctccag ta
//
LOCUS       DAZPAB010000088        15146 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZPAB010000088 DAZPAB010000000
VERSION     DAZPAB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15146
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.088"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..178
                     /gene="yjbD"
                     /locus_tag="ACHUPG_002408"
     CDS             <1..178
                     /gene="yjbD"
                     /locus_tag="ACHUPG_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR0861998.1"
                     /translation="VLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEA
                     SFSWSANTSTRGRR"
     gene            complement(311..1183)
                     /gene="rluF"
                     /locus_tag="ACHUPG_002409"
     CDS             complement(311..1183)
                     /gene="rluF"
                     /locus_tag="ACHUPG_002409"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR0861999.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1531..2478
                     /locus_tag="ACHUPG_002410"
     CDS             1531..2478
                     /locus_tag="ACHUPG_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFR0862000.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2548..3354
                     /locus_tag="ACHUPG_002411"
     CDS             2548..3354
                     /locus_tag="ACHUPG_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0862001.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3364..3771
                     /locus_tag="ACHUPG_002412"
     CDS             3364..3771
                     /locus_tag="ACHUPG_002412"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR0862002.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3771..4265
                     /locus_tag="ACHUPG_002413"
     CDS             3771..4265
                     /locus_tag="ACHUPG_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFR0862003.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4331..5128
                     /locus_tag="ACHUPG_002414"
     CDS             4331..5128
                     /locus_tag="ACHUPG_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR0862004.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            5139..5927
                     /locus_tag="ACHUPG_002415"
     CDS             5139..5927
                     /locus_tag="ACHUPG_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IID"
                     /protein_id="HFR0862005.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5979..7208
                     /gene="sorE"
                     /locus_tag="ACHUPG_002416"
     CDS             5979..7208
                     /gene="sorE"
                     /locus_tag="ACHUPG_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFR0862006.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7590..8279
                     /gene="pepE"
                     /locus_tag="ACHUPG_002417"
     CDS             7590..8279
                     /gene="pepE"
                     /locus_tag="ACHUPG_002417"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFR0862007.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8370..10001)
                     /locus_tag="ACHUPG_002418"
     CDS             complement(8370..10001)
                     /locus_tag="ACHUPG_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR0862008.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10221..13904)
                     /gene="metH"
                     /locus_tag="ACHUPG_002419"
     CDS             complement(10221..13904)
                     /gene="metH"
                     /locus_tag="ACHUPG_002419"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR0862009.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14104..14928
                     /gene="iclR"
                     /locus_tag="ACHUPG_002420"
     CDS             14104..14928
                     /gene="iclR"
                     /locus_tag="ACHUPG_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR0862010.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3602 a         3993 c         3947 g         3604 t
ORIGIN      
        1 cgtactgacc aactccgaaa ccaacagcat taagaaagag tacatcgata aattaatggt
       61 cgagcgtgag gctgaagcga aaaaagcccg ccaattgaag aaaaagcagg cttataaacc
      121 ggatcctgaa gcgtcattta gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg
      181 aacaatcaac gcctgagatt ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa
      241 ccctctcccc gaaggggcga ggggactgtc cgagctcttt ttggacgttt tcatcagtct
      301 gaggcgttaa tcagcgtccc ttctttttac gccccggcga ggtaaaacgc ttaccgttgg
      361 acgccgggcg accgcctttt tccgccgttt tttccatctt aacgactgga cgtttgatgc
      421 ccgctgtttt cggtttggcc ttcgctttag gttttgcctc ggaagaggaa ttttcgatga
      481 gcttaaagag gtcgattaac tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc
      541 cgcttaagct gacgttcata atgcgcgtac gttccagctt tttcacttca tagccgaaat
      601 gctcgcacat gcgccggatc tgacggttca gcccctgcac tagggtaatg cggaagacaa
      661 acggcgcttc ttttttgact ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg
      721 cccccatacc acgaataaat tcatcggtaa tcggtttatc gaccgtcacc agatactctt
      781 tctcatgatc gttacccgca cggaggatct tattcaccag atcgccgtga ttggtgagga
      841 aaatcagccc ctgggagtct ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt
      901 taacgaaatc gacaatgtta tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg
      961 gcttgttcag ggcgataagt accaaatctt cggcttcccg aggctcaatc aactgaccgt
     1021 ttactttcac aatgtcgccg ggtttcacct gatcgccaat ggtggctcgc ttgccattaa
     1081 ggaacacatt gccttgctcg atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt
     1141 cgctgatgta tttatttaaa cggactgatg agtcgggcag cataaattct cctgtaaaac
     1201 gcggaatata ccgtacctct tagcggggaa aaacagggtt atgcggcaac gtcgaaatta
     1261 aatgtgatct gccacgcctt ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc
     1321 ctggcaaaat aaggataatc aacatattac gaatcgctgc acacctgtgc tgatgactac
     1381 tacgcagaga aacaaatgtg caagagaaag cgctatttct ccccatgcaa agagtgaaaa
     1441 atcgctttga aaggccctca tgatggggtt atatcagatc aagcggtgag aaagcgtatt
     1501 tgcacaaatg tgcagaacca ggaggcggca atggaaaaca gtgacgatat ccgtttgatt
     1561 gtgaagattg cccaactcta ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa
     1621 ctgggtattt accgcaccac catcagccgc ttgcttaaac gaggccgcga tcagggaatg
     1681 gtcaccatcg ccatcaacta tgactacaac gaaaatctct ggctggagca gcaactgaag
     1741 caaaagtttg gcctgaaaga cgttgtggtg gtgtcgggaa atgatgagga tgaagagact
     1801 caactggcga tgatggggtt acacggcgcg caactgctgg atcgcttgct ggagcctggc
     1861 gatattgtcg gtttttcctg gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag
     1921 gcggggcaat cgcggcagtt aatctgcgta ccaattattg gcggaccgtc cggtaaactc
     1981 gaaagccgct atcatgtaaa cacattaacc tacagcgcgg cagcgaagct gaaaggggaa
     2041 tcgcatctcg cggattttcc ggctctgctg gataacccat taattcgtaa tgggatcatg
     2101 cagtctcagc actttaaaac catctctgcc tactgggata atctggatat cgccctggtg
     2161 ggaattggct caccggccat tcgcgacggc gctaactggc atgcgtttta tggtggtgaa
     2221 gagagtgacg acctgaatgc ccgccaggtt gctggcgata tttgctcgcg cttttttgat
     2281 attcacggcg caatggttga aacgaatatg agcgaaaaaa cgctctctat cgaaatgaat
     2341 aaattaaagc aggcgcgata ttccattggc attgccatga gtgaagaaaa atacagcgga
     2401 attattggtg cactgcgtgg aaaatatatt aattgtctgg taacgaatag cagcacagct
     2461 gaactattac tgaaataaaa atcttcccga tgtttaccgt aataaaaatt acggcgggtc
     2521 cctattatct aaaatcagga gtctgttatg caaacgtggt taaatttgca ggataaaatc
     2581 attattgtca ccggcggcgc atccggtatt ggtctggcga ttgtagagga attattagca
     2641 caaggcgcga atgtacagat ggtcgatatt cacggtggcg atggtcaata tgaaggtcat
     2701 aaaggctatc agttttggcc gaccgatatt tctagcacaa aagaggtaaa tcatacggta
     2761 gcggaaatta tccagcgttt tggtcgcatc gacggtctgg tcaataacgc cggggtcaat
     2821 ttcccgcgtc tgctggtcga tgagaaagcg cctgccgggc agtatgaact caacgaagct
     2881 gcattcgaaa aaatggtcaa tatcaaccag aaaggtgttt ttctgatgtc gcaggcggtg
     2941 gcgcgacaga tggtcaaaca acatgatggc gtgattgtga atgtttcctc agaaagtggg
     3001 ctggaaggct cagaaggaca aagctgttac gccgctacca aagccgcgct caatagcttc
     3061 acgcgctcct ggtcgaaaga gctgggtaaa cacggtatcc gtgtggtcgg tatcgcgccc
     3121 gggattctgg aaaaaaccgg gctacgcacg ccggaatatg aagaggcgct ggcgtggacg
     3181 cgcaatatca ccgtcgagca gctgcgtgaa ggctatacca aaaacgccat tcctattggg
     3241 cgcgccggaa gattagcaga agtagctgat tttgtttgtt atctgttgtc tgaacgcgcc
     3301 agctacatca ccggagtaac cactaacatt gcgggcggca aaacgcgcgg ctaaggaggc
     3361 tttatggtca acgccatttt ttgtgcccac ggcaaattag cttgcgccat gctggaatcg
     3421 gttcagatgg tttacggcaa cgccaatgtt gaagccgttg catttgtgcc cggtgaaaac
     3481 gccggagaca ttgcggtaaa gctggaaaag ttagtaagca ttcacaatca agatgagtgg
     3541 ctgattgccg tggatttaca gtgcggcagc ccgtggaatg cggcagcaat gcttgcgatg
     3601 cgcaatcctc gtttgcgggt aatcagcggc ctttctctgc cgctggcgct cgaactggtt
     3661 gataaccagg acagcatgaa tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag
     3721 caaacctgcg ttgtctggaa acaactggca acgacagagg aggatttctg atgaacatta
     3781 cgctcgcccg cattgatgac cgtctgatcc acggccaggt cactaccgtc tggtcgaaag
     3841 tggctaacgc ccagcgcatt attatctgca atgacgaagt ttataacgat gaagtgcgcc
     3901 ggacattatt gcgtcaggct gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa
     3961 aagccgtcgc ggtttatcac aacccgcaat atcaggacga aaccgttttt tatcttttca
     4021 ctcgcccaca ggatgcctta gcgatggttc gccagggggt aaaaataggc accctgaata
     4081 ttggcggcat ggcctggcgg cccggtaaaa aacagttaac caaagcggtt tcattagatg
     4141 atgatgatat taacgcattt catgagttaa ataatcttgg tgtcatactg gatttacgcg
     4201 tcgtagcgtc agatccctca atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa
     4261 attaaataat agcgcctgtg tacatatgct ttaacaggca gggatgacga cacttaaaag
     4321 gtgacaaatc atggaaataa gtaccctaca aatcattgct atatttcttt tttcctgtat
     4381 tgccggaatg ggcagcgtgc tggatgaatt tcagactcat cgcccgttaa ttgcctgtac
     4441 ggtgattggt ttaattctcg gtgatttaaa aaccggaata atactcggcg gtacgctgga
     4501 attgatagct ctctgctgga tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc
     4561 cagcataatc tccgccattc tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc
     4621 catcgcgttg cctgtggctg cggcaggcca ggtgctgacg gtgtttgccc gtaccatcac
     4681 cgtagtgttc cagcacgcgg cggataaagc agcagaagag gcgcggtttc gcactctcga
     4741 tattctgcat gtctccgcgc ttggcgtgca ggcgctgcgc gtcgccattc cggcactgat
     4801 tgtctcactg ttcgtcagcg ccgatatggt cagcaatatg ttaaacgcca ttcccgaatt
     4861 tgtgacccga ggactacaga ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt
     4921 gctacgtatg atgggcgtga aatatttgat gcctttcttt ttcctcggtt tcctcgcagg
     4981 tggctacctc gatctcagcc tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt
     5041 ctacatccag ctaaatccac agtggtgtaa agctgaacct caactccagg ccaccgcctc
     5101 caccgccctt gaccaacttg acgattaacg gagccatcat ggaacagaga aaaattacac
     5161 gcagcgatct ggtgagcatg tttctgcgct ccaacctgca acaggcgtcc tttaactttg
     5221 aacgtattca cgggctgggc ttttgctacg acatgatccc cgccatcaag cgactttacc
     5281 cattaaaaga ggatcaggtt gcggcgctca ggctacacct ggtgttcttc aataccacgc
     5341 cagccgtatg tggcccggtc atcggcgcgg aaattgatga cggtaccatc aacggcatca
     5401 aagtcggtct gatgggacca ttggcaggag ttggcgatcc actggtctgg ggaacgctgc
     5461 gcccgattac cgccgcgctc ggcgcatctc tggcactttc gggcaacatt ctcggcccgc
     5521 tgctgttctt ctttattttc aacgcggtgc gtctggcgat gaagtggtat ggcctacagc
     5581 tcggctttcg caaaggggtg aatatcgtca gcgatatggg cgggaatgtg ctgcaaaaac
     5641 tcaccgaagg cgcgtcgatt ctcggactgt ttgtgatggg cgtgctggtg accaaatgga
     5701 cgtcaatcaa cgtaccgttg gtggtttcac aaacgcatgc cgccgatggc tccaccgtca
     5761 ccatgaccgt gcagaacatt ctcgaccaac tttgccctgg tttgctggcg ctcggtctga
     5821 cgctactaat ggttcgtctg ctcaacaaaa aaattaaccc ggtatggctg attttcgccc
     5881 tgtttggctt agggattatc ggcaatgctc tgggcttcct gtcctgattc ttcgccccgg
     5941 cacgactgcc gaggccatcg ctcaacatga ggtggtttat gaaaacaaca gctctgcgtc
     6001 tttatggtaa acgtgattta cgcctggaaa cctttgacct tcctgaaatg caggaggatg
     6061 aaatcctcgc aacggtggtc actgacagcc tgtgcctctc ttcctggaaa gaggccaatc
     6121 tgggtgaaaa ccataaaaaa gtacccgacg atgtgacgac caacccaatc atcatcggcc
     6181 acgaattttg cggcgatatt ctggccgtgg gtaaaaagtg gcagcacaaa ttccagccgg
     6241 gtcagcgtta tgtgattcag gccaacctgc aactccccga ccgcccggat tgccccggct
     6301 actccttccc gtgggtagac ggcgaagcga cgcatgtggt tattcccaac gaggtcatgg
     6361 aacaatattg cctgctggca tacgacggcg aaacctattt tgaaggctca ctggttgaac
     6421 cgctttcctg cgtgattggc gcgttcaacg ccaactatca tcttcaggaa ggtagttata
     6481 accacacgat ggggattcgc ccgcaagggc acacgctgat cctcggcggc accggaccaa
     6541 tgggactgtt ggcgattgat tatgcgctgc atggaccagt taacccgtcg ctgctcgtca
     6601 ttaccgatac cgacaacgac aaattgagtt atgcgcgcaa gcactatcca tcagaaccgc
     6661 aaacactgat tcattatctc aatgccaccg atgcagcatt tgatacgtta atggcgctga
     6721 gtggcggtca cggcttcgat gatattttcg tctttgtgcc taatgaagga ctggtgactc
     6781 tcgcctcttc cttgctggcg acagatggtt gcctgaattt cttcgccgga ccgcaggata
     6841 aacatttcag cgcgccaatt aatttctacg atgtgcatta tgcatttacc cactacgtgg
     6901 gcacgtcagg cggcaatacc gacgacatgc gcgcggcggt caaactgatc gaagagaaaa
     6961 aagtgcaggc cgcaaaagtg gtaacacata ttcttgggct gaatgccgcg ggcgaaacca
     7021 cgcttgaatt gcctgccgtc ggcggcggca aaaagctggt gtataccggg aaatacctgc
     7081 cgctgacgtc actcacgcag attcaggatc aagcactggc ggcgattctg gcgcgtcatc
     7141 aggggatctg gtccggtgag gtggagcaat acctgctcgc ccatgcagag gcaatttccc
     7201 atgattaatc gcgctactta actctgcatg tcgctggctg gtcgccccag caacttcgcg
     7261 accggatgtg cggctgattg ctgaagcagc acaatttgca cgagaaggat aaaaaatatc
     7321 agcgccttaa ggggcgctca aattggtggc aaagtacaca ttgtttatgc cggatgcgga
     7381 atgaagcctt attcggtcta cataattttg aaaatccaat atattgcagt tatctggtag
     7441 gccaggtaag cgtagcgcat caggcaatgc tgtgtttgtc atcagtctca gcgcccggtg
     7501 caatacgtga catcgtgagc catgtcagta agtggttaaa ttcgcagtgg tagcatgcct
     7561 tccgtgattt cttactaacc ggagaagtaa tggaactgct tttattgagt aactcgacgc
     7621 tgccgggtaa agcctggctg gaacatgcac tgccgctaat tgctgaacag ttgcagggtc
     7681 gccgctcagc ggtgtttatc cctttcgctg gcgtaacgca gacctgggat gattacacag
     7741 agaaaacggc tgcggttctc gctccgctgg gtgtttctgt caccggtatt catagcgttg
     7801 tcgatcccgt tgccgcgatt gaaaatgcgg aaattgtgat tgtcggcggc ggtaatactt
     7861 tccagttgct gaaacagtgc cgcgagcgcg ggctgctggc tccaattact gacgtggtta
     7921 aacgtggcgc gctgtatatt ggctggagcg caggcgctaa ccttgcttgc ccaaccattc
     7981 gtaccaccaa cgatatgccg attgtcgatc cgcaaggttt cgatgcgcta aatctgttcc
     8041 cgttgcaaat caacccgcac ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc
     8101 gtgagcagcg tattcgcgaa ctgctggtcg tcgcgccaga actgacgatt attggtctgc
     8161 cggaaggtaa ctggatcaca gtgagtaaag gtcacgctac gctgggtggc ccgaacacca
     8221 cttatgtgtt taaggctggt gaagaagcgg ttccgctgga agctggtcac cgtttttaag
     8281 tgaattgccg gatgcatgat ccggcatctt gttcatagtg cctgatgcga cgcttgacgc
     8341 gtcttatcag gcctacagga ttcacaatat taatactcgt cccgtccttc atcttcatcc
     8401 ggctgttcca gcacactgta agccaccgaa caaaacagcg agttcaggcg ctgcatatct
     8461 cccagtaagc ctaaatgtag cgaactggtt tcaatgctct gcacgttttg ctgatgcagg
     8521 cgatcgacgt gggcgtggga atagcggcga ttaagaatgc gaaaacgatg tttgctgcga
     8581 cgcaaacgac gagcgctggt gacatcgcca gagaagaaca ccgacattgc cagctttaaa
     8641 ttactgagca attgctcata gagcgcatcc agttccttca acccatcaag cgaaaatgcc
     8701 cgccgtgctg ccagcgattt atcggcaatt tcgctgccca tgcgctcgac gatatcggag
     8761 gcctgttcaa ggttgagcga catttcgatg atctccgccc agcggcgcga ctcttcctcc
     8821 gccaactctt cttttggcat ccgcgccaga tacagcttaa tggcggtgta gagaacgttg
     8881 atatcatctg ccagcttacg cagctctttc tcctgccgtg gctcgccgtg catcacttta
     8941 ttcagccctt ccatcatctg ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg
     9001 ttcgccagag caagcgtggg cgtgtccagc gcgctgacat ccagatgttt aggccgtagc
     9061 tgggtatcca gttccggttc atcgcgaata atcgttttgc aaaaccgtgc catcgggtca
     9121 acaaatggca gcatgaccag gcaacgtaca aggttgtaga agacgtggaa atagatcacc
     9181 agttccgctt ttggcaatga cagcttcccc attgtctctg ccagcaaatg gacaaacggc
     9241 aggataatca ggctacccac cagcttaaac agcagactac ccagcgcgac acggcgggct
     9301 gcggcattgg cggcactgtt gttgagcatc gccagcaggc cggaaccgag gttagctcca
     9361 atcaccagac agagcgccac ggggaaggag ataatgcctg cagcggtcag agtcgcggtc
     9421 agcagtacag cagcaaggct ggagtagctg ataatggcga acatcgcgcc aatcagcgca
     9481 tccagcagaa tatcgccggt cagcgaggca aagatcacct gaacgccgtt tgcctgggtg
     9541 atcggcgtta cggcctgcac aatcaactcc agcgccagca aaatcagccc aagaccaata
     9601 ccgacgcggc ccagttgccc ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg
     9661 ataaaaataa gtaacggtga cagccaggat aagtcgaagg tgagaatacg cgccattagc
     9721 gccgtcccga catctgcccc cagcacaatg accagagccg gtgcgagggc taccagatcc
     9781 tgagcgacga acgaggtcac cagcatggtg gtggcattac tgctctgtac cagtgcggta
     9841 acgccgatcc ccgcgcaaaa ggcgagcggc tttttttcga cgctccggct aaggacagta
     9901 cgcaaacgcg cgccgaagac gcgcattacg ccggttcgaa caatatgagt cccccagacc
     9961 agcagggcga cggcagaaag caggtgaagc agcgttaaca cgagttcaga ttctccttat
    10021 cgttatatgt tcctgaagca attgaatgac caaaggaccg gaaacacgct gactggcatc
    10081 tttactgtgc cttttgacat tcgtttttcc ggctaagatt attccagcct gtttccagta
    10141 taagtgtttc atattaataa agagacaggg cactcattga gcgccctgtt tgttgtaagg
    10201 aaaagtgaca gatttgtgaa tcagtccgcg tcatacccca gattcggtgc cagccagcgc
    10261 tcaacatcgg agacgctcat acctttacgg cgggcatggt cttcaacctg atcgcgctga
    10321 atttgcgcca ccgcgtagta cttgctgtcc ggatgactga aataccaacc cgaaaccgat
    10381 gcaccaggcc acatcgcgaa agattctgtg agtttcatgc cagtatgttt ttccacttcc
    10441 agcagctccc agatggtggc tttttccgta tgttccgggc aggccggata gcccggtgcc
    10501 ggacggatgc cctggtagtt ttcgcggatc agttcttcgt tgctgaggtt ctcgttcggc
    10561 gcatagcccc agtagacttt acgcacacgc tcatggagat actccgcaaa ggcttcggct
    10621 aaacggtcgg caagcgcttt caccatgatt ttgttgtaat catcgtgctg cgcttcaaag
    10681 gcatcagcca gtgcgtcctc ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct
    10741 gctttaccag aaagcttcgg cgcaacgaag tcagcgagac agtagttagc gaatcccgtt
    10801 ttttcggtct gttgacgcag atggtggctg acgttgatca catgggtgcg cgtttcgtca
    10861 cggtagattt caatgtcatc gcccacacgg tttgccggga acaggcccac cacgccacgc
    10921 ggattcagcg ttttctcggc gcttaattta tccagcatgt cgttggcgtc tttaaacagc
    10981 cgctgcgcct caacgcccac cacttcatct tccagaatgc gaggatactt cccggccagc
    11041 gaccaggtca taaagaacgg tgtccagtcg atgtaattac gcagcgtttc gatgctggct
    11101 tccacttcct gcacgccgag acggtgcgcc accggcggcg tgtaagcctg ccagtcaaat
    11161 gcgaaatcgt tatcgcgcgc cgcttccagc gtgaccggtg gtgtgcgtgg tttcttgcgc
    11221 ccgtgctgaa tacgcacggt ttcgtactcc ttgcaggtac gagcgacaaa atcatcacgc
    11281 tgagtatcgg aaagcagcgc cgctaccaca ccaacggtgc gcgaggcatt ctgcacatac
    11341 accgtagggc cgctgtagtt ctgctcgatt ttcaccgccg tgtgcgcttt tgaggtggtc
    11401 gcgccgccaa tcagtaacgg aatggcgaag ccctgacgct ccatctcttt cgccacgtta
    11461 accatctcgt ccagcgacgg cgtgataagc cccgaaaggc caatcaaatc agcattcact
    11521 tctttggcga tacggagaat tttttccgca ggcaccataa cgccgagatc gacaatttcg
    11581 tagttgttac attgcagcac cacaccaacg atatttttac cgatgtcgtg gacatcgcct
    11641 ttcacggtgg cgatcaccat cttgccgttg gttttaccct gctctttgct ggcttcaata
    11701 aacggttcga gatacgccac tgcctgtttc atgacgcgcg ccgatttgac cacctgtggc
    11761 aggaacattt tgccttcgcc aaacagatcg ccgaccacgt tcatgccgtc catcaacggg
    11821 ccttcaatca cttcaatcgg gcgcgtagcc tgctggcggg cttcttcagt atcctgctcg
    11881 ataaactcgg ttatgccttt gaccagcgag tattccagac gtttattcac gtcccacgag
    11941 cgccactccg cttgctgggc gttagcagtg tcgtccgctt tactgccgcg atatttctcg
    12001 gcaagctcca gtaaacgctc agtgccatcg tcacgacgat taagaatcac atcttccacc
    12061 gcgtcgcgca gttcagcggg taggtcgtcg taaatcgcca gttgcccggc gttgacgatc
    12121 cccatatcca tgccattgcg aatagcgtag tagaggaaca ctgcgtgaat ggcttcgcgc
    12181 accggatcgt tgccacggaa tgagaaagaa acgttagata cgccgccgga aatcagcgcg
    12241 tgcggcagtt cgcgtttgat gtcttcacac gcgccgataa agtcctgcgc gtagttgttg
    12301 tgctcttcaa taccggtggc gaccgcgaag atgttcgggt cgaagatgat atcttctggc
    12361 gggaagccaa cctcttcggt gaggattttg tacgcccgac ggcaaatctc gattttccgt
    12421 gcgcgagtat cggcctgtcc ctgttcgtca aaggccatca ccaccactgc cgcaccgtag
    12481 cgacgcaaca atttcgcgtg atggataaag gcgtcgacgc cctctttcat cgagatagag
    12541 ttaacaatgc ctttgccctg gatacacttc agaccttttt caatgacatc ccattttgag
    12601 gagtcgatca taatcggcac gcgagcgata tccggttcac cggcaatcag attgagaaaa
    12661 cgcaccatcg ccgcttcggc atcgagcatc ccttcatcca tgttgatatc gataatctgc
    12721 gcgccgtttt ccacctgctg acgcgccaca tccagcgcct cgctgtattt ctcttctttg
    12781 atcaggcgct tgaacttagc agagccggtg acgttggtgc gttcacccac gttcacaaac
    12841 aggctatcgt cgccaatgtt cagcggctcc aggccagaca aacggcaggc tacgggaatt
    12901 tccggcagtt tgcgcggcgc taatccttct actgcacgac tcatcgctgc aatatgttgc
    12961 ggcgtggtcc cgcagcaacc accgacgata ttgagaaagc ccgcttgcgc ccattcacgt
    13021 atctgttttg ccatcgtgtc ggcgtcgaga tcgtattcac caaaggcgtt aggcagcccg
    13081 gcgttcgggt gggcggtgac gtagcattcc gcaatccgtg atagttcctg cacgtactgg
    13141 cgcagttcat cgggccccag cgcacagttc aggccaaagg tcagcgcttc ggcgtggcgc
    13201 aatgagttgt aaaatgcttc ggtggtctgc ccggagagcg tgcgcccgga ggcgtcggtg
    13261 atggtgccgg agatcataat cggcagctca acgcccagcg cttcaaactc cgttttcacc
    13321 gcaaagaccg ccgctttggc gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc
    13381 gcaccacctt ccaccagcgc tttggtggat tcgcgatagg ccgccgccag ctggtcaaaa
    13441 gtgatgttgc ggaatgccgg atcgttgacg tccggagaaa tagacgccgt gcggttggtt
    13501 gggccaagaa caccggcaac gtagcgcggt ttctccggcg tgcgcgcggt ccactcgtca
    13561 gcacaggcgc gcgccagttt cgccgccgca aagttgattt ccgccgacag ggattccatc
    13621 tggtaatccg ccatcgcaat ggtcgtggag ttgaaggtgt tggtttcgat gatatccgcg
    13681 cccgcttcaa agtaggcgtt gtggatagcg gcgatcactt ccggtttact gagtaccagc
    13741 aggtcgttgt tgcctttgag gtcgcatggc cagtcggcaa agcgttcacc acgaaaatcg
    13801 gcttcgttca gtcgatagct ctggatcatg gtgcccatac cgccgtccaa caccagaata
    13861 cgttcattta actgcgcacg cagttgttcc actttgctgc tcacacttgc tcccgacacg
    13921 cttaacccag atttaataaa aattcaacaa accatactgg aataaacgca tctgtggtaa
    13981 aagcgaccac cacgcaacat gagatttgtt caacattaac tcatcggatc agttcagcaa
    14041 ctattgcatt agctaacaat aaaaatgaaa ataatttcca cgatacagaa aaaggaggct
    14101 gtcatggtcg cacccattcc cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca
    14161 gcgactggac aggttcagtc tttaacgcgt ggcctgaaat tactggagtg gattgccgaa
    14221 tccaatggca gcgtggcact cacagagctg gcgcaacaag ccgggttacc caattccacg
    14281 acccaccgcc tgctaaccac gatgcaacag cagggtttcg tgcgtcaggt cggcgaactg
    14341 ggacactggg caatcggcgc acatgccttt atggtcggca gcagctttct ccagagccgt
    14401 aatttgttag caattgttca ccctatcctg cgcaatctaa tggaagagtc tggcgaaacg
    14461 gtcaatatgg cggtgcttga tcaaagcgat cacgaagcga ttattatcga ccaggtacag
    14521 tgtacccacc tgatgcgaat gtccgcgcct atcggcggta aattgccgat gcacgcttcc
    14581 ggtgcgggta aagccttttt agcccaactg agcgaagaac aggtgacgaa gctgctgcac
    14641 cgcaaagggt tacatgccta tacccacgca actctggtgt ctcctgtgca tttaaaagaa
    14701 gatctcgccc aaacgcgcaa acggggttat tcatttgacg atgaggaaca tgcgctgggg
    14761 ctacgttgcc ttgcagcgtg tattttcgat gagcaccgcg aaccgtttgc cgcaatttct
    14821 atttccggac cgatttcacg tattaccgat gatcgcgtga ccgagtttgg cgcgatggtg
    14881 attaaagcgg cgaaggaagt gacgctggcg tacggtggaa tgcgctgact ttttctggcg
    14941 ggcagaggca atattctgcc catcatacct gagtggcaat agaataaggg tgtctgttaa
    15001 tcgcattgac gccaaaataa cttaatgtca tacacttcac acacttttat taactcagca
    15061 ttatttttaa acatcaaacc acttaattat taacaaaatc gcaagatctc attccaccga
    15121 aacttttacg gctgcattat gagatt
//
LOCUS       DAZPAB010000089        15137 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZPAB010000089 DAZPAB010000000
VERSION     DAZPAB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15137
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.089"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..579
                     /locus_tag="ACHUPG_002421"
     CDS             <1..579
                     /locus_tag="ACHUPG_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0862011.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="ACHUPG_002422"
     CDS             597..1235
                     /locus_tag="ACHUPG_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0862012.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="ACHUPG_002423"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="ACHUPG_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR0862013.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACHUPG_002424"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="ACHUPG_002424"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR0862014.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="ACHUPG_002425"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="ACHUPG_002425"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR0862015.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACHUPG_002426"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="ACHUPG_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR0862016.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="ACHUPG_002427"
     CDS             complement(5552..6613)
                     /locus_tag="ACHUPG_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR0862017.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="ACHUPG_002428"
     CDS             complement(6610..8007)
                     /locus_tag="ACHUPG_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR0862018.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="ACHUPG_002429"
     CDS             8163..9161
                     /locus_tag="ACHUPG_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0862019.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACHUPG_002430"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="ACHUPG_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="ACHUPG_002431"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="ACHUPG_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="ACHUPG_002432"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="ACHUPG_002432"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR0862020.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="ACHUPG_002433"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="ACHUPG_002433"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR0862021.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..15137)
                     /locus_tag="ACHUPG_002434"
                     /pseudo
     CDS             complement(14232..15137)
                     /locus_tag="ACHUPG_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
BASE COUNT         3695 a         4144 c         3638 g         3660 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagc
//
LOCUS       DAZPAB010000090        14935 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZPAB010000090 DAZPAB010000000
VERSION     DAZPAB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.090"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            48..290
                     /gene="yjbE"
                     /locus_tag="ACHUPG_002435"
     CDS             48..290
                     /gene="yjbE"
                     /locus_tag="ACHUPG_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR0862022.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            404..1042
                     /locus_tag="ACHUPG_002436"
     CDS             404..1042
                     /locus_tag="ACHUPG_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR0862023.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1039..1776
                     /locus_tag="ACHUPG_002437"
     CDS             1039..1776
                     /locus_tag="ACHUPG_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR0862024.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1776..3872
                     /locus_tag="ACHUPG_002438"
     CDS             1776..3872
                     /locus_tag="ACHUPG_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR0862025.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3919..4197)
                     /locus_tag="ACHUPG_002439"
     CDS             complement(3919..4197)
                     /locus_tag="ACHUPG_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862026.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4198..4341
                     /locus_tag="ACHUPG_002440"
     CDS             4198..4341
                     /locus_tag="ACHUPG_002440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862027.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4467..4877
                     /gene="psiE"
                     /locus_tag="ACHUPG_002441"
     CDS             4467..4877
                     /gene="psiE"
                     /locus_tag="ACHUPG_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR0862028.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4921..6396)
                     /gene="xylE"
                     /locus_tag="ACHUPG_002442"
     CDS             complement(4921..6396)
                     /gene="xylE"
                     /locus_tag="ACHUPG_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR0862029.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6767..7657)
                     /gene="malG"
                     /locus_tag="ACHUPG_002443"
     CDS             complement(6767..7657)
                     /gene="malG"
                     /locus_tag="ACHUPG_002443"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR0862030.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7672..9216)
                     /gene="malF"
                     /locus_tag="ACHUPG_002444"
     CDS             complement(7672..9216)
                     /gene="malF"
                     /locus_tag="ACHUPG_002444"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFR0862031.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9370..10560)
                     /gene="malE"
                     /locus_tag="ACHUPG_002445"
     CDS             complement(9370..10560)
                     /gene="malE"
                     /locus_tag="ACHUPG_002445"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR0862032.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10925..12040
                     /gene="malK"
                     /locus_tag="ACHUPG_002446"
     CDS             10925..12040
                     /gene="malK"
                     /locus_tag="ACHUPG_002446"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR0862033.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12112..13452
                     /gene="lamB"
                     /locus_tag="ACHUPG_002447"
     CDS             12112..13452
                     /gene="lamB"
                     /locus_tag="ACHUPG_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR0862034.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13604..14524
                     /gene="malM"
                     /locus_tag="ACHUPG_002448"
     CDS             13604..14524
                     /gene="malM"
                     /locus_tag="ACHUPG_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR0862035.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14753..>14935
                     /locus_tag="ACHUPG_002449"
     CDS             14753..>14935
                     /locus_tag="ACHUPG_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862036.1"
                     /translation="MIGHITNSGGNNSLMKHDVVQGNNIVDLDLLRNFNGVTGLNRDY
                     FIYISDIFLNIKQRNEK"
BASE COUNT         3722 a         3821 c         3913 g         3479 t
ORIGIN      
        1 aaatgttttt gggtaaatct ccattcattc aatgaaggga aattgttatg aaaaaagttc
       61 tgtatggcat ttttgccata tctgcgcttg cggcgacttc tgcgtgggct gcacctgtac
      121 aggtgggcga agcggcagag tcggcagcaa cgtcggtttc ggcggggagt tcctccgcga
      181 ccagcgtcag caccgtaagc tcggcggtgg gtgtcgcgct cgcggcaacc ggtggcggtg
      241 atggttctaa taccgggacc accacaacca cgaccaccag tacccagtaa taaagtatgt
      301 atccccaaaa taatttgagt cattgcatct gtggcttgaa gtatgaaggg atttaccata
      361 accacactcc ggtgtggtta ttctgcccct ctggagaaga gtcgtgaagc gacctgcact
      421 cattcttatc tgcctgctat tacaggcctg ttcagccacg actaaagagc tgggcaattc
      481 actgtgggac agtctgttcg gcacgccagg cgtacagctg acggacgatg atattcaaaa
      541 tatgccctac gccagccagt acatgcagct taatggcggg ccgcaattgt ttgtggtgct
      601 ggccttcgct gaagacggac aacaaaaatg ggtcactcag gatcaagcca ctcttgtcac
      661 acaacatggt cgtctggtga agactttgct tggcggcgac aacctgattg aagtgaataa
      721 ccttgccgcc gacccgctga ttaaacccgc acaaattgtt gatggtgcaa catggacgcg
      781 cacgatgggc tggaccgagt accagcaggt acgctacgcc accgcacgct cagtcttcaa
      841 atgggatggc accgataccg tcaaggtcgg cagcgatgaa accctggttc gcgtgctgga
      901 cgaagaagtc tccaccgacc aggcgcgctg gcataaccgc tactggatcg acagcgaagg
      961 gcaaattcgc cagtcggaac agtatctcgg cgcggattat ttcccggtga aaaccactct
     1021 catcaaggcg gcaaaacaat gattaaacaa actattgtcg cgttgctttt gagtgtggga
     1081 gcgtcatcgg tcttcgcggc aggaaccgtc aaggtgttca gcaatggcag cagtgaggcc
     1141 aaaacgctaa cgggcgcaga gcatttaatc gatctggtag ggcaacctcg actggcaaac
     1201 agctggtggc ctggtgcggt gattagcgaa gagctggcaa cggcggcagc attgcgtcag
     1261 cagcaggcgt tgctgacacg gctggcagaa cagggcgcag attccagcac cgacgatgcc
     1321 gccgccatta acgccttacg ccagcaaatc caggcattga aagtgacagg caggcaaaaa
     1381 atcaatcttg atcccgatat cgtccgcgtt gccgaacgcg gtaacccgcc gttgcagggc
     1441 aactacacgc tgtgggtcgg accaccgccg tccacggtca cgttgttcgg gcttatcagc
     1501 catcctggca atcagccatt cactccaggt cgcgacgtgg cgagctatct ctctgaccag
     1561 agcctgctca gcggtgcgga tcgcagctac gcgtgggtcg tttacccgga cggacgcacg
     1621 caaaaagcgc cggtggctta ctggaacaag cgtcacgtag agccgatgcc cggcagcatt
     1681 atttatgttg gcctcgcaga ttccgtctgg agtgagacgc ctgatgccct taacgccgac
     1741 attcttcaga ctctgacgca gcggataccc caataatgaa aaaaagacat ctgcttagct
     1801 tactggcgct gggcattagc acagcttgct acggcgaaac atatcctgcg cccattggtc
     1861 cgtcgcagtc ggatttcggt ggcgtaggat tattacaaac tcccaccgcg cgtatggcac
     1921 gggaagggga gttgagtctg aactatcgcg ataacgatca gtaccgttat tactcagctt
     1981 cagtgcaact cttcccgtgg ctggaaacaa cgctgcgcta caccgacgtg cgcacccggc
     2041 agtacagcag cgtcgaagcg ttctctggcg atcaaacgta taaagataaa gccttcgatc
     2101 tcaaactgcg tttgtgggaa gagagttact ggctgccgca agtggcggtt ggtgcgcggg
     2161 atatcggcgg tacggggctg tttgatgcgg aatatcttgt tgccagcaaa gcctgggggc
     2221 cgttcgattt tacgctcggc ctgggctggg gatatctggg caccagcggt aatgtaaaaa
     2281 atccgctctg ttcagccagt gataaatatt gctatcgcga taacagctac aaacaggcgg
     2341 gatctatcga cggtagccag atgttccacg gtcctgcctc actgtttggc ggcgtggaat
     2401 accagacgcc ctggcaaccg ctgcgtctga aactggagta tgaaggtaat aattatcagc
     2461 aggattttgc cgggaagctg gagcaaaaaa gtaagtttaa cgtcggggcg atttatcgcg
     2521 ttaccgattg ggccgacgtt aaccttagct atgaacgtgg caacaccttt atgtttggcg
     2581 tcacgctgcg caccaacttt aacgatctgc gcccgtccta catcgataac gcccgcccgc
     2641 aatatcaacc gcagccgcag gatgccattt tgcagcattc ggtggtggcg aatcagttaa
     2701 cgttgctgaa atacaacgcc ggacttgccg atccgcagat ccaggcgaaa ggcgatacgc
     2761 tgtatgttac cggcgagcag gtgaaatatc gtgattcgcg cgaagggatc atccgtgcta
     2821 atcggatcgt gatgaacgat ctgccggatg ggatcaaaac gatccgcatt acggaaaatc
     2881 gccttaacat gccgcaagtg acgacggaaa ccgatgtcgc cagcctgaaa aatcatctcg
     2941 ccggagagcc gttgggccac gaaacgacgc tggcgcacaa acgcgtcgag ccagtggttc
     3001 cgaagtccac cgagcagggc tggtatatcg acaaatcacg ctttgatttc catatcgatc
     3061 cggtgctgaa ccagtcggtc ggtggcccgg aaaactttta catgtatcag ctgggcgtga
     3121 tgggaacggc agatttgtgg ctgacggacc atctactgac caccggcagc ctgtttgcaa
     3181 atcttgccaa caactacgac aagtttaact acaccaatcc accgcaggac tcgcacttac
     3241 cgcgcgttcg tacccatgtg cgcgagtatg tgcagaacga tgtctatgtg aataacctgc
     3301 aagccaacta cttccagcat ctgggcaacg gcttctacgg tcaggtctat ggaggttatc
     3361 tcgaaaccat gtttggcggt gcgggggccg aagtgttgta tcgtccgctg gacagtaact
     3421 gggcgtttgg tctggatgcc aactacgtta aacagcgcga ctggcgtagt gcaaaagata
     3481 tgatgaaatt caccgactac agcgtgaaaa ccggacatct gaccgcctac tggacgccat
     3541 ctttcgccca ggacgtgttg gtgaaagcca gcgtcgggca gtatctggca ggggataaag
     3601 gcggcacgct ggagatcgct aaacgctttg acagcggcgt ggtggtgggt ggctatgcca
     3661 cgatcaccaa cgtttcgaaa gaggagtacg gcgaagggga cttcaccaaa ggcgtgtatg
     3721 tctcggtacc gttggatctc ttctcgtctg gcccgacacg cagccgtgcg gcgattggct
     3781 ggacgccgct gacgcgtgac ggtggtcagc aacttgggcg taagttccag ttgtatgaca
     3841 tgaccagcga ccgtagcgta aatttccgct aagtcatggg aaaggtgcca gttttcgcac
     3901 tcactggcac cctcattctt aatgacctgt tgagccatca ggaacgcagt tcacccccgg
     3961 tttacgatct ggtgtgtaaa ggcgaaaaca ttctgtcttc ctgtccgtcg atatcggttg
     4021 caggtctggt tgcctgagcg actgggccat cattgggctg gagacattgc tttgcggtag
     4081 gtcggcttta tcagcagtac cgagcggacc agcataagca ggaagaacag agactgataa
     4141 catcaaagca gcaaaataag gcttcatttt taccaccttt atcaggttac gtttcatttg
     4201 ttccagagga acattgtcga tttttcgcgc attgctggtg gctgggaatc acctgaatgg
     4261 gtgatttttg aattaccggc tttggtgcgg tttgtcttgc cggatgcgcc gccaggcgcg
     4321 gcttatccgg cctacgggta ggtatatccg gctttggtgg aggcgcgctc caaatccagg
     4381 ttgaacaaaa catacacaaa aaatatagat ctccgtcaca tttttgcgtt atacaggaag
     4441 ctcgccactg tgaaggaggt actgctatga cgtcactctc tcgtccgcgc gtggagttta
     4501 tctccaccat tttgcagacc gtactcaatc ttggcctgct gtgcctcggc ctgattttgg
     4561 ttgtcttcct cggcaaagaa acggtgcatc tggctgatgt gctgttcgcg ccagaacaaa
     4621 ccagcaaata tgagctggta gaaggactgg tggtttactt tctctatttc gaatttatcg
     4681 cgctgattgt gaagtacttt cagtccggtt ttcacttccc gctacgttac tttgtctata
     4741 ttgggatcac cgccattgtg cgcttgatca tcgtcgatca taaatcgcca ctggatgtgt
     4801 tgatctactc ggctgcgatc ctgttactgg tgatcaccct gtggctgtgt aattcgaaac
     4861 ggctgaagcg ggagtaaaaa gacagcacgc cgaaatggcg cggcgtgctg gacaggaaga
     4921 ttacagcgta gcagtttgtt gtgttttctt cgtttccggt tcccagagcg cttccagctc
     4981 ctcaagggtt ttacctttgg tttccgggac aaatttccac ataaacagtg ctgccagaac
     5041 gcccatacaa ccgtaaatcc agtaggagaa accgttgtgg aaatgggcca ccagccagga
     5101 gtttttgtcc atcatcggga aggtccagga gacgaagtag ttcgccagcc actgggccgc
     5161 caccgcgatt gccagcgctt taccacgaat agcattcggg aagatttccg acagcagtac
     5221 ccagcatacc ggaccccagg acatggcaaa ggcggcaaca tagaacagca tcgacagtag
     5281 cgccacaata cccgatgcct gagtgtaaaa cgcggtaccg aggctaaaca taccgattgc
     5341 cattccgagt gcgccgataa tttgcagtgg cttacgacca aatttatcca cagtcataat
     5401 tgccagtacg gtaaaggtga ggttgataac tccgacaata atggtctgca acagcgcgat
     5461 atccgtgctg gcccccagcg ttttgaacac ttccggcgcg tagtacagca ccacattgat
     5521 gccgacaaat tgctggaaga tggagagcat aacgccgatt acaatcacgc ccacgccaaa
     5581 catcagcaga cgaccaccgg ttttgcggcc atgatccagg gagtgtttaa tttcctgtac
     5641 tgcctgagtt gcaagcgtgt tgcccataat tttgcgcagg atactttccg tctgttcttg
     5701 cttgccgcgt gacatcagcc agcgaggact ttctggcacg gtatacagca gcattaagaa
     5761 cagcagtgca gggatacatt ccgaggcaaa catataacgc ctgccgtcag tattcagcca
     5821 gctggcatca ccggaacggg caataaaata gtttacgcag taaactaaaa gttgcccgaa
     5881 aataatcgca aactggttaa atgaaaccag ttttccgcga atatgagctg gagccagttc
     5941 cgcaatatac attggtgaaa gcattgaggc taaaccaacg ccaataccgc caataatacg
     6001 ataaataaca aattccggga cataacctgc cagataaata ggtactgtgt tgtccgggtt
     6061 tatagaggta aaaccaagtt ctggccaggc agaacctaca ccagaaataa aaaacaggac
     6121 agcagaaatc ttaagtgaat cacgacggcc gaagcggtta ctgcaataac caccgagggc
     6181 accgccgatg atgcaaccaa tcagagcgct ggctacgcaa aatcctaaca gggagttggc
     6241 agcggattca cttaagtttt gtggagcaac aaagacggta ttgagtgact caacagtacc
     6301 ggaaataacg gcggtgtcgt agccaaataa taaaccacct aatgtagcga ctaaggtaat
     6361 cgaaaatata taactggaat tatactgggt attcattcag acctgcctta gaccattctg
     6421 atcttttatc agtgcattga tgttggaatt gtcaggcagt taaggcaatt atcatttttt
     6481 acaatgcgtt tacgtaatgt cttttctgtg atcttaattg tgataattat caaaaattga
     6541 tgaaacaata ctattgccgt gactcagagc acgaaagaga attatcgtaa gtgggaaaac
     6601 aaataacgta aaaataataa gttctatgat gggaatatta accggcgaac gattcagatt
     6661 gcagacgaaa gaaaaaaagg cgctccgtgg agcgccgaat aatagtcaca agttgggata
     6721 acgtaagttg agggtgcagc ggcataacat tggcagaaca acatctttaa cctttcacac
     6781 cacctgccgt caggccgttg accagccagc gttgagccag caagaagacg atggtgatcg
     6841 gtaatgcaga catcacggca gcggcggcaa agtcacccca caggtagttt tgcgggttga
     6901 ggtattgctg catccccacg gccagggtgt agctgtttac gtcacgcagt aacagcgacg
     6961 cgaccggaac ttcagtaatg gcagcgataa acgacaggat gaataccacc gccagaatcg
     7021 gtactgacag cggcaacagg acaaggcgga aggcctgcca cggtgtcgca ccatccagcg
     7081 cagccgcttc ttccagcgaa ctgtcgatgg tttcgaaata gcctttgatg gtccagacat
     7141 gcagcgcaat cccacccaga tacgcgaaaa ttacgccgcc gtgagtattc aggccaatga
     7201 atggaatgta ctcacccaga cgatcaaaca acgcatacaa cgcgaccagt gaaagtactg
     7261 ccgggaacat ctggaaaatc agcattcctt tcagcagcgt cgctttgcct ggaaagcgca
     7321 tacgggcgaa agcgtaggcg caggtggtgg agagcgccac aatgccaatc gcggaaatcc
     7381 cagcgacctt taccgagttc cacagccaca gcagtaccgg gaatggcggt ggcgtaatgc
     7441 gaccatcagc ctgttcaacg ctaaaaccta acgccagttt ccagtgatcc caggagattt
     7501 gctccgggat caggctaccg gtcgcgaagt ttccctgacg cagcgagata gcgacgacca
     7561 tcagcagcgg gaacataatc gccgcgataa aaagtagcag tagcaggtga gtaataaaca
     7621 aacgtgcttt ttgcgatttc ggttggacca ttgccatttt tgttatctcc cttaatcaaa
     7681 cttcattcgc gtggctttca ggttcactat cgccagcgca cccaccagca ggaagatcag
     7741 cgtggcaatt gctgccgcca gaccgaagtc ctgacccccg ccgccttcaa aagcgatacg
     7801 gtaggtgtag ttaacaagca ggtcggtata accggctggc gtggtcgtgc caagacgatc
     7861 cgggccgccg ttggttaaca gctgaatcag cacgaagttg ttaaagttaa aggcgaagct
     7921 ggcgatcatc agcggcgtca gcggtttaat cagcagcggc agcgtaatct taaagaagtt
     7981 ctggaacgga cctgcgccat ccattgctga ggcttcatac aaatcgtccg gaatcgcttt
     8041 cagcaagccc atgcagagga tcatcatgta cggataaccc agccaggtat tgacgataat
     8101 cagcatcgtg cgagcggtgg tcggatcgct gaaccaggcg ggcttcacgc caaacagcgc
     8161 gctcaacatc atgttgattt caccgaagct ctggttaaac aatcctttga aaatcaagat
     8221 tgaaatgaac gatggcaccg cgtagggcag aatcagcagg acgcgataga ccgctttgcc
     8281 gcgcaacgct tcccactgca ccagacatgc cagaaccatg ccgaccgcca ccgttaaaaa
     8341 gacagtgatc agcgagaaca ccacggtcca gacgaaaatg gcgaggaacg gtttctgaat
     8401 gccttcgtcg gtaaagacgc gggtaaagtt tttccagccg gtggtcacgg tgtaaccggg
     8461 gcttagcttt tcatcccccc agttgccgtc ggcggtaatg gactggtaaa agccaatttg
     8521 gttattcgga cgatatttca cgccactctg attattcgtc aacgtgccgt caccgtcgag
     8581 tgtgtagagc ggctgcgtgc cagaaaactg gcgcagggag ctcatcatca ctttgttgcc
     8641 atccggcaga atggcggtaa tgtcactcag cgcctgacga ttctgggtaa tcacgcgcag
     8701 attcgcgcgt tcgccttcgg gctgggcggt cgtttctttc agttgcagtt tttgctcgcc
     8761 gccaaattta aacgcgtcgg agaggtaatt tttgccggtt tcgccgtcgc tgagcgccag
     8821 ttgccactca tcgcccgccg ggtaaagacc aaagttatag gttttgcctg cttgccagga
     8881 gcgatctaac aacacttcct gcgcacgttc aaaagtcagc tggttagtgc tgctgtagtt
     8941 ggtgaaggca atggcgatgg tgcagaccag agggaagagg acgaataatc ccattccagc
     9001 cattcccggg taaacatagc gccaggcgta ggctttacga ttggcgaaaa tatacagccc
     9061 cgctgaactc aatatcagcg tggtaatggc gaacaggtat tccccttgtg cgtacattaa
     9121 aacaacaagg taacccacca gcaggccgag cagacctagc actgaccatt tcagcgcgtc
     9181 gctttgccac caatgtttct ttttaatgac atccatgggg ttcttcctca ttccaggacg
     9241 gataaggctt tcacgcctta tccgacaaca actgcctgat gcgacactga tgcgtcttat
     9301 caggcctaca tgcgtttcag ttttgtaggc cggacaaggc gttcacgccg catccggcat
     9361 ttcacagcat tacttggtga tacgagtctg cgcgtctttc agggcttcat cgacagtctg
     9421 acgaccgctg gcggcgttga tcaccgcagt acgtacggca taccagaaag cggacatctg
     9481 cgggatgttc ggcatgattt cacctttctg ggcgttttcc atggtggcgg caatacgtgg
     9541 atctttcgcc aactcttcct cgtaagactt cagcgctacg gcacccagcg gtttgtcttt
     9601 attaaccgct tccagacctt catcagtcag cagatagttt tcgaggaact ctttcgccag
     9661 ctctttgttc ggactggcgg cgttaatacc ggtgctcagc acgccaacga acggtttaga
     9721 tggttgaccc ttgaaggtcg gcagtaccgt tacaccataa ttcactttgc tggtgtcgat
     9781 gttggaccat gcccacgggc cgttgatggt catcgctgtt tcgcctttat taaaggcagc
     9841 ttctgcgatg gagtaatcgg tgtctgcatt catgtgtttg tttttaatca ggtcaaccag
     9901 gaaggtcaga cccgctttcg cgccagcgtt atccacgccc acgtctttaa tgtcgtactt
     9961 gccgttttca tacttgaacg cataaccccc gtcagcagca atcagcggcc aggtgaagta
    10021 cggttcttgc aggttgaaca tcagcgcgct cttacctttc gctttcagtt ctttatccag
    10081 cgccgggatc tcttcccagg tttttggcgg gttcggcagc aggtctttgt tataaatcag
    10141 cgataacgct tcaacagcga ttgggtaagc aatcagcttg ccgttgtaac gtacggcatc
    10201 ccatgtaaac ggatacagct tgtcctggaa cgctttgtcc ggggtgattt cagccaacag
    10261 gccagattga gcgtagccac caaagcggtc gtgcgcccag aagataatgt cagggccatc
    10321 gccagttgcc gcaacctgag ggaatttctc ttccagttta tccggatgct caacggtgac
    10381 tttaattccg gtatctttct cgaatttctt accgacttca gcgagaccgt tatagccttt
    10441 atcgccgtta atccagatta ccagtttacc ttcttcgatt ttggcgagag ccgaggcgga
    10501 aaacatcatc gtcgttaatg cggataatgc gaggatgcgt gcacctgttt ttattttcat
    10561 aatctatggt ccttgttggt gaagtgctcg tgaaaacacc taaacggact ctagtttctt
    10621 tatacggcaa cctctttcca tcctccttgc ccctacgccc caccgttgct ttgtgtgatc
    10681 tctgttacag aattggcggt aatgtgggga tgcgcacata aaatcgccac gatttttgca
    10741 agcaacatca cgaaattcct tacatgacct cggtttagtt cacagaagcc gcgttctcat
    10801 cctcccgcct cctcccccat aaaaaagcca gggggtggag gatttaagcc atctcctgat
    10861 gacgcatagt cagcccatca tgaatgttgc tgtcgatgac aggttgttac aaagggagaa
    10921 gggcatggcg agcgtacagc tgcaaaatgt aacgaaagcc tggggcgagg tcgtggtatc
    10981 gaaagatatc aatctcgata tccatgaagg tgaattcgtg gtgtttgtcg gaccgtctgg
    11041 ctgcggtaaa tcgactttac tgcgcatgat tgccgggctt gagacgatca ccagcggcga
    11101 cctgttcatc ggtgagaaac ggatgaatga cactccgcca gcagaacgcg gcgttggtat
    11161 ggtgtttcag tcttacgcgc tctatcccca cctgtcagta gcagaaaaca tgtcatttgg
    11221 cctgaaactg gctggcgcaa aaaaagaggt gattaaccaa cgcgtcaacc aggtggcgga
    11281 agtgctgcaa ctggcgcatt tgctggatcg caaaccgaaa gcgctctccg gtggtcagcg
    11341 tcagcgcgtg gcgattggcc gtacgctggt ggccgagcca agcgtatttt tgctcgatga
    11401 accgctctcc aacctcgatg ctgcactgcg tgtgcaaatg cgtatcgaaa tctcccgtct
    11461 gcataaacgc ctgggccgca caatgattta cgtcacccac gatcaggtcg aagcgatgac
    11521 gctggccgac aaaatcgtgg tgctggacgc cggtcgcgtg gcacaggttg ggaaaccgct
    11581 ggaactgtac cactatccgg cagaccgttt tgtcgccgga tttatcggtt cgccaaagat
    11641 gaacttcctg ccggtaaaag tgaccgccac cgcaatcgat caagtgcagg tggagctgcc
    11701 gatgccaaat cgtcaacaag tctggctgcc tgttgaaagc cgtgatgtcc aggttggagc
    11761 caatatgtcg ctgggtattc gcccggaaca tctactgccg agtgatatcg ctgacgtcat
    11821 ccttgagggt gaagttcagg tcgtcgagca actcggcaac gaaacccaaa tctatatcca
    11881 gatcccttcc attcgtcaaa acctggtgta ccgccagaac gacgtggtgt tggtagaaga
    11941 aggtgccaca ttcgctatcg gcctgccgcc agagcgttgc catctgttcc gtgaggatgg
    12001 cactgcatgt cgtcgactgc ataaggagcc gggcgtttaa gcaccccaca aaacacacaa
    12061 agcctgtcac aggtgatgtg aaaaaagaaa agcaatgact caggagatag aatgatgatt
    12121 actctgcgca aacttcctct ggcggttgcc gtcgcagcgg gcgtaatgtc tgctcaggca
    12181 atggctgttg atttccacgg ctatgcacgt tccggtattg gctggacagg tagcggcggt
    12241 gaacaacagt gtttccagac taccggtgct caaagtaaat accgtcttgg caacgaatgt
    12301 gaaacttatg ctgaattaaa attgggtcag gaagtgtgga aagagggcga taagagcttc
    12361 tatttcgaca ctaacgtggc ctattccgtc gcgcaacaga atgactggga agctaccgat
    12421 ccggccttcc gtgaagcaaa cgtgcaggga aaaaacctga tcgaatggct gccaggttcc
    12481 accatctggg caggtaagcg cttctaccaa cgtcatgacg ttcatatgat cgacttctac
    12541 tactgggata tttctggtcc tggtgccggt ctggaaaaca tcgatgttgg cttcggtaaa
    12601 ctctctctgg cagcaacccg ctcctctgaa gctggtggtt cttcctcttt tgccagcaac
    12661 aatatttatg actataccaa cgaaaccgcg aacgacgttt tcgatgtgcg tttagcgcag
    12721 atggaaatca acccgggcgg cacattagaa ctgggtgtcg actacggtcg tgccaacctg
    12781 cgtgataact atcgtctggt tgatggcgca tcgaaagacg gctggttatt cactgctgaa
    12841 catactcaga gtgtcctgaa gggctttaac aagtttgttg ttcagtacgc tactgactcg
    12901 atgacctcgc agggtaaagg tctgtcgcag ggttctggcg tcgcgtttga taacgaaaaa
    12961 tttgcctaca atatcaacaa caacggtcac atgctgcgta tcctcgacca cggtgcgatc
    13021 tccatgggcg acaactggga catgatgtac gtgggtatgt accaggatat caactgggat
    13081 aacgacaacg gcaccaagtg gtggaccgtt ggtattcgcc cgatgtacaa gtggacgcca
    13141 atcatgagca ccgtgatgga aatcggctac gacaacgtcg aatcccagcg caccggcgac
    13201 aagaacaatc agtacaaaat taccctcgca caacaatggc aggctggcga cagcatctgg
    13261 tcacgcccgg ctattcgtgt cttcgcaacc tacgccaagt gggatgagaa atggggttac
    13321 gactacaacg gcgatagcaa ggttaacccg aactacggca aagccgttcc tgctgatttc
    13381 aacggcggca gcttcggtcg tggcgacagc gacgagtgga ccttcggtgc ccagatggaa
    13441 atctggtggt aatagcaaaa cctgggccgg ataaggcgtt tacgccgcat ccggcataaa
    13501 aacaggttgt cattatctga aaggggcgaa agcccctctg attatcgggt ttagcgcgct
    13561 attgcctggc taccgctgag ctccagattt tgaggtgaaa acaatgaaaa tgaataaaag
    13621 tctcatcgcc ctctgtttat cagcagggtt actggcaagc gcgcctggaa ttagccttgc
    13681 cgatgttaac tacgtaccgc aaaacaccag cgacgcgcca gccattccat ctgccgcgct
    13741 gcaacaactc acctggacac cggtcgatca atctaaaacc cagaccaccc aactggcgac
    13801 cggcggccaa caactgaacg ttcccggcat cagtggtccg gttgctgcgt acagcgtccc
    13861 ggcaaacatt ggcgaactga ccctgacgct gaccagcgaa gtgaacaaac aaaccagcgt
    13921 ttttgcgccg aacgtgctga ttcttgatca gaacatgacc ccatcagcct tcttccccag
    13981 cagttatttc acctaccagg aactaggcgt gatgagtgca gatcggctgg aaggcgttat
    14041 gcgcctgaca ccggcgttgg ggcagcaaaa actttatgtt ctggtcttta ccacggaaaa
    14101 agatctccag cagacgaccc aactgctcga cccggctaaa gcctatgcca aaggcgtcgg
    14161 taactcgatc ccggatattc ccgatccggt tgctcgtcat accagcgatg gcttactgaa
    14221 actgaaagtc aaaacgaact ccagctccag cgtgttggta ggaccgctat ttggttcttc
    14281 cgctccagct ccggttacgg taggcaacac ggcggcacca gctgtggctg cacctgctcc
    14341 ggtgccggtg aagaaaagcg agccgatgct caacgacacg gaaagttatt ttaataccgc
    14401 gatcaaaaac gctgtcgcga aaggtgatgt tgataaggcg ttaaaactgc ttgatgaagc
    14461 tgaacgcctg ggatcgacat ctgcccgttc cacctttatc agcagtgtaa aaggcaaggg
    14521 gtaattacgc cccacagtgc tgattttgca acaactggtg cgtctcctgg cgcacctttt
    14581 tttatgcttc cttcctggga tatgagcgat tttttatagt acttcacttc ttcttcacta
    14641 agaatgtcca ttatttcaat gcattatcag agattctttt cctttcgccg gtagtgtctg
    14701 gacgctcagg ctacttttcc aggttatttt atttctgtat tacagaggtt ttatgatagg
    14761 tcatatcaca aattctggcg gcaataactc tttgatgaaa catgatgtgg tgcaaggaaa
    14821 taatatagta gatcttgatt tactacgtaa tttcaatggg gtgacaggtt taaacagaga
    14881 ttactttatt tatatcagtg atatattttt aaatataaaa caacggaacg aaaaa
//
LOCUS       DAZPAB010000091        14693 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZPAB010000091 DAZPAB010000000
VERSION     DAZPAB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.091"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..1259)
                     /locus_tag="ACHUPG_002450"
     CDS             complement(<1..1259)
                     /locus_tag="ACHUPG_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0862037.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPL"
     gene            complement(1457..1532)
                     /locus_tag="ACHUPG_002451"
     tRNA            complement(1457..1532)
                     /locus_tag="ACHUPG_002451"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1497..1499),aa:Phe,seq:gaa)
     gene            complement(1638..2345)
                     /locus_tag="ACHUPG_002452"
     CDS             complement(1638..2345)
                     /locus_tag="ACHUPG_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR0862038.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2498..2581
                     /gene="yqgH"
                     /locus_tag="ACHUPG_002453"
     CDS             2498..2581
                     /gene="yqgH"
                     /locus_tag="ACHUPG_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR0862039.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2743..4878
                     /gene="speC"
                     /locus_tag="ACHUPG_002454"
     CDS             2743..4878
                     /gene="speC"
                     /locus_tag="ACHUPG_002454"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR0862040.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4927..6183)
                     /gene="nupG"
                     /locus_tag="ACHUPG_002455"
     CDS             complement(4927..6183)
                     /gene="nupG"
                     /locus_tag="ACHUPG_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR0862041.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6385..7464)
                     /gene="mltC"
                     /locus_tag="ACHUPG_002456"
     CDS             complement(6385..7464)
                     /gene="mltC"
                     /locus_tag="ACHUPG_002456"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR0862042.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7529..7804)
                     /locus_tag="ACHUPG_002457"
     CDS             complement(7529..7804)
                     /locus_tag="ACHUPG_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR0862043.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7832..8884)
                     /gene="mutY"
                     /locus_tag="ACHUPG_002458"
     CDS             complement(7832..8884)
                     /gene="mutY"
                     /locus_tag="ACHUPG_002458"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR0862044.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9045..9764
                     /gene="trmB"
                     /locus_tag="ACHUPG_002459"
     CDS             9045..9764
                     /gene="trmB"
                     /locus_tag="ACHUPG_002459"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR0862045.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9764..10090
                     /locus_tag="ACHUPG_002460"
     CDS             9764..10090
                     /locus_tag="ACHUPG_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR0862046.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10274..10993
                     /locus_tag="ACHUPG_002461"
     CDS             10274..10993
                     /locus_tag="ACHUPG_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR0862047.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11169..12215
                     /gene="ansB"
                     /locus_tag="ACHUPG_002462"
     CDS             11169..12215
                     /gene="ansB"
                     /locus_tag="ACHUPG_002462"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR0862048.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12332..13294
                     /locus_tag="ACHUPG_002463"
     CDS             12332..13294
                     /locus_tag="ACHUPG_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR0862049.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(13395..>14693)
                     /locus_tag="ACHUPG_002464"
     CDS             complement(13395..>14693)
                     /locus_tag="ACHUPG_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFR0862050.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3833 a         3547 c         3789 g         3524 t
ORIGIN      
        1 tttaatggat tgttaatctt tgcatattca aacgggctga tacccttctc ccggttcgca
       61 tctagaaaat cggcccacca ttgcaacatc agtcgccgtt cttccagatg ttctgcttta
      121 tggatatacg cagcacgtac accattacgc tcctgatggc tcatctgacg ttccacagca
      181 tcgcgggacc acaaacctga ttcaatcaat gcactgcacg ccatcgcccg gaatccatgg
      241 ccacagactt cctgggttgt gtcatacccc atcaccctca gggctttatt tacagtattt
      301 tcactgattg gtttacgcgg gtcatgtgca cccgtaaaga tcagaccatt ttcaccagcc
      361 catgtctgaa gttctgccag tatggctaca acctgtttgc tgagtggcac aagatgtggt
      421 gttcgcatct tggatccccg gtgtgagaat tttacgcctg gcagaggttc gcgttcggct
      481 ggaatagtcc acatggaatt gtcaatatca atttccgacc agcgggcata tctgagttca
      541 ctggaacgaa taaaaaccag taaattcagc ataactgcca gtcgggtgac aggctggcct
      601 ttataaccgt caatacgcga cagaagttca ggcaggcgtg aaagctccag agcagggcga
      661 tgctggcgtt ttaccgtggt caatgcacca gccatatcca gagccgggtt atatctgatg
      721 atgccagact gtacggcgta acgcattaca gcagatattc tttgctgcag acgactggcg
      781 atttcataaa tttgttttgc ttctgcagca cgaacaggga taagcaggtc cggggtattg
      841 agtgttgtga tatcccggtt gccgataaag gggaaaacgt gtgtctcgag gcttttgagt
      901 actttatcgc tgtgtgattg tgcccattgt ttattactgg catgccagcg tcgtgcaata
      961 tgttcaaaag agagctgtaa atcagggaca acttttttaa ctcttttctg ttcgcaaggg
     1021 tcaatcccct ctgaaatcag ttttcgggcc tcatcccgtt ttgtacgagc ttcggagaga
     1081 gaaacttctg gatacacacc aagtgccaga gtcttctcct tacccagaat acgataacgg
     1141 agacgccagt aacgggaacc attaggatga accagcagga acatgccggc cccgtcagtg
     1201 agtttataag ccttgtcagt gggctttgca gcccggattt ttgcgtcagt cagtgccata
     1261 gttcagcctt ctgaatgcgt taaagagggt acaaattctg tcgaactggg gtatacccgc
     1321 aattataccc acacaatggg gttgatgtgg ggggaatctg gttgagttcg gtagaattga
     1381 tttggaggaa gaacacttaa atcatggtgt tctgaaacga aaaacggacc tccgtggagg
     1441 tccgttgata tgaatttggt gcccggactc ggaatcgaac caaggacacg gggattttca
     1501 atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc tgattcgcct
     1561 cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc atcactttgc
     1621 cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag cggcattgcc
     1681 agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca gatgcgtaat
     1741 ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat cgacggtgtg
     1801 atcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat cagtaatggg
     1861 atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc cgtaaagaaa
     1921 tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc gttcatcgcc
     1981 ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata attctgaata
     2041 aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc tttggcgact
     2101 gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc aagtagcgta
     2161 gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc aatccccagc
     2221 gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa gcgttggctg
     2281 agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa agggccgatg
     2341 accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact ttttgccttg
     2401 ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag gtgccatgat
     2461 tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct ccttacattc
     2521 tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc tcatgcggtg
     2581 aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg cttttactga
     2641 tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca gggttttcca
     2701 ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc aatgaatatt
     2761 gccgccagta gtgaactggt atcccgactt tcttctcatc gccgcgtggt ggcgttggga
     2821 gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag tcgcagtggc
     2881 attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta ttccgaacat
     2941 gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca gcagtggctg
     3001 gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt ttatgacacg
     3061 ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca tcaacatggt
     3121 gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg tgagaacgtc
     3181 tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat tcatgaagga
     3241 tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa aacctatttt
     3301 gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt aacgcgtggc
     3361 gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc gctgattcag
     3421 gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt cattggcggt
     3481 attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga cgttgcgcca
     3541 gaaaaagccg acctgccgcg cccgtttcgc ctgacgatta ttcagctggg aacctatgac
     3601 ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctgtg tgattacatt
     3661 ctgtttgatt ccgcgtgggt cggttacgaa cagtttatct cgatgatggc ggatagctcg
     3721 ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca gttggtgcac
     3781 aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa ccatatccgc
     3841 ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct ccatgcttct
     3901 accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat tcatgaaggg
     3961 gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc gcgcaaggct
     4021 attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga tggcaaattg
     4081 tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag ttttgagccg
     4141 ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt tgatccgtgc
     4201 aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag cgactttggc
     4261 gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc ggagaagtgc
     4321 gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa gctggcacaa
     4381 ctggtggcga tgctggcgca atttgaacag catattgagg atgactcgtc gctggctgag
     4441 gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac cctgcgccag
     4501 ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct acaaaaagcg
     4561 atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc gcatagcgct
     4621 tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg aattgcggca
     4681 gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga agtctggggt
     4741 ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt gccgggattt
     4801 tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa acgattgtac
     4861 ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc gtttttcttt
     4921 gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt tatatttgaa
     4981 catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca accatacggt
     5041 ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc tcacgatgcc
     5101 gccgaggata cagccgaagc cgttagtcat catcaggaac atcccttgtg cactggcgcg
     5161 aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga agaagtcgaa
     5221 tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg gagtcgggtc
     5281 gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca tcattacgtt
     5341 cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg tttcagagat
     5401 ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg caaacatcgg
     5461 atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct gcagttccgc
     5521 gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa acagcgcgaa
     5581 tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag caaccggaat
     5641 atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc caatatacag
     5701 ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca tgataaagcc
     5761 gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc cggcattttg
     5821 caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag caaacgagtt
     5881 aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca tgaacagcgt
     5941 gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc atttgtcggc
     6001 cacaatcccc agcagcgcag gcataaatac cgcggcgata cccagtgagc tataaactgc
     6061 gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga gggtcgtcag
     6121 ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca gcttaagatt
     6181 catgttaatt tcctcacatc gtgatgcgga tgagtgtttg aaaaagcaca tttgaccgtg
     6241 cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt ggcaaataat
     6301 tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt tgcccctgag
     6361 catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg cggtattcac
     6421 tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg tctgataaac
     6481 atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat tcgaaaagac
     6541 tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac gccgcgatgt
     6601 tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg cggtgccggt
     6661 atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg atttcccctg
     6721 cgagcggaac acatctttcc cggcagtatt ttgtaccacc tgcattaatc ccagcgcatc
     6781 ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg ccagaatcag
     6841 cgactcatca acgccatatt tccgtgacgc ctggcggacc atgccgagat atttgtgcgc
     6901 acgtttatca aggtggttcg gcaccatgtt aatggtgacg ctgtagatga tacgtagccc
     6961 gttgctgcgg cttttcagac ggtttttcag cagataatcc gcgaagttgc ttgcgcgacc
     7021 ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga aaggttcttt
     7081 cgaaattgta atatcatcaa cgtcggaata gagatcgacc gaactcggat cgtcacccat
     7141 cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc cggcgatggt
     7201 ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat attgatcggt
     7261 gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc agatgttctc
     7321 aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt tgacccaggc
     7381 ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg gcgcaatcaa
     7441 agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt tttatgcaag
     7501 ctgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta tagccctcga
     7561 tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc agcttgcggt
     7621 gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt ttgtgctgcc
     7681 actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc tcgccggggt
     7741 acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa atcgttctgc
     7801 tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg cgtaactgct
     7861 gtaacaaacg ctccacggga gccgccaggc caactgacgg cggttgcgct aagttatacc
     7921 agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc cacataggca
     7981 caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc ggtcagttgc gtcaggttat
     8041 cggcagcaat ctgccgttgt gccagccaat gccgcaaact ttcttcgtcg gcaaactgcg
     8101 ggaaacagta taaaccgccc cacaatccgc tcggcggacg ctgcgccagc aatacttcat
     8161 cttcgtgctg taacaggaga aagtagccgg tgcgctccgg cagcgtctgt ttcggttttt
     8221 tgcccggata aagcgaccag ctattgttgg tggcggcaat acatccgttt tgtagcggac
     8281 agagcgaaca tttcggcttc gaacgcgtgc aaatcatcgc gcccaaatcc atcatcgcct
     8341 gattaaaccg ttccacgccg accgcgggcg tcacctgctc gctcaaactc catagtttat
     8401 tctcgacctc ttttttccca ggccagccgc ttacagcata gcagcgcgcc agcacgcgtt
     8461 tgacgttacc gtcgagaatc ggaaagtgct tacccagaga aagcgagaga atcgcgcctg
     8521 cggtggaacg cccgacgccc ggtaacgccg cgacttcttc aaaggtttcc gggaatttac
     8581 caccgtgtaa ggtcgccact tgttgtgccg ctttatgcag gttgcgcgcg cgggcgtaat
     8641 agccaagccc ggtccacaag tggagaactt cgtcgagcgg cgcattggcg agatcggtca
     8701 ccgtcgggaa gtgcgccata aagcgttcaa aataggggat aacggtcgca acctgagttt
     8761 gttgcaacat cacttctgag agccatactt tgtagggcgt cttgtcaatt tgccagggca
     8821 gcgttttccg cccgtattta tcgtaccagt ccagaacctg ggctgaaaat tgcgacgctt
     8881 gcatggtcac cgacttcact gttgttgggg gcaagattgc agcacagcga cggcagggtg
     8941 taaaccggaa ctttccgcag ccacggcctt atcatgcttg catccggcaa ctaactttgg
     9001 ataatgcccg ttttcagaac actttcacaa gcgactaaat ctttatgaaa aacgacgtca
     9061 tttcaccgga atttgatgaa aacggccgcc cactgcgccg tatccgtagt tttgtgcgcc
     9121 gccaggggcg actgaccaaa ggccaggaac atgcgctgga aaactactgg ccggtgatgg
     9181 gcgttgagtt cagtgaagat atgctggatt tccccgcgct ttttggccgt gaagcgccgg
     9241 tgacgcttga gattggtttt ggcatggggg cgtcgctggt ggcaatggct aaagatcgcc
     9301 ccgagcagga cttcctcggc attgaagtgc attcaccggg cgttggtgcg tgcctggctt
     9361 ctgcgcatga agaaggttta agcaacctgc gcgtgatgtg tcacgatgcg gttgaagtgc
     9421 tgcataaaat gattcctgac aattcattgc gcatggtgca gctctttttc cctgacccgt
     9481 ggcacaaagc gcgccataat aaacgccgta tcgttcaggt gccgtttgcc gaactggtaa
     9541 aaagcaaact gcagctgggg ggcgtattcc atatggcgac cgactgggaa ccttatgcgg
     9601 aacatatgct tgaagtgatg tcttctattg acggttataa aaacctgtca gagagcaatg
     9661 attacgtacc gcgtccggca tcacgtccgg tgacgaaatt tgaacaacgt ggtcatcgtc
     9721 ttggtcacgg agtatgggac ttaatgttcg agagggtgaa ataatggcaa agaaccgtag
     9781 ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag gaattaggat tttcggtggc
     9841 atggcgattc ccggaaggta catcggaaga acagattgat aaaatcgttg atgactttat
     9901 taacgaggtt atcgaaccga acaaactggc ctttgacggc agcggttatc tggcctggga
     9961 aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa gaacatcagg cgattgtgcg
    10021 taagtggctg gaagagtgca aactggatga ggtacgcacc agcgaacttt tcgacgtttg
    10081 gtgggactaa gaaagcatac gggcgatgac aaatgcaaaa ctgcctgatg cgctacgctt
    10141 atcaggccta cagagatgca cgatcgagta ggcgggataa ggcgtttacg ccgcatccgg
    10201 catggaaaac gcgtactttg ttatcaatct ggggccagca tatgctggcc tgatttgttc
    10261 ttgagggaag actatgatgc gcaaaatgct gctggcggca gcactttcag tgacggcaat
    10321 gaccgctcac gccgactacc agtgcagcgt cacgccgcgt gacgatgtga ttgtcagccc
    10381 gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg atcacgccag acggcaacgt
    10441 gatgtataac ggtaagcaat attccctgaa tgccgcccag cgcgagcagg cgaaggatta
    10501 tcaggctgaa ctacgtagca cgctgccgtg gattgatgaa ggcgcgaaaa gccgcgtcga
    10561 gaaagcccgt attgcgctgg ataaaattat cgttcaggag atgggcgaca gcagcaaaat
    10621 gcgcagccgt ctgaccaaac ttgatgcgca gcttaaagag cagatgaatc gcattatcga
    10681 aacgcgcagc gatggcctga cgttccacta taaagccatt gatcaggttc gcgccgaagg
    10741 ccagcaacta gtgaatcagg caatgggcgg aattttacag gacagcatta atgaaatggg
    10801 cgcgaaagcg gtgctgaaaa gcggcggtaa cccattacag aatgtgctgg gaagcttggg
    10861 cggtctgcaa tcctcaatcc aaaccgagtg gaaaaagcag gaaaaagatt tccagcagtt
    10921 tggcaaagat gtttgtagcc gcgttgtgac tctggaagat agccgcaaag ccctggtcgg
    10981 gaatttaaaa taatcctcta ttttaagacg gcataatact tttttatgcc gtttaattct
    11041 tcgttttgtt acctgcctct aactttgtag atctccaaaa tatattcacg ttgtaaattg
    11101 tttaacgtca aatttcccat gcaaagctaa gggataatgc gtagcgtcca cgtaactgga
    11161 ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc actggttatg ggttttagtg
    11221 gtgcagcatt ggcattaccc aatatcacca ttttagcaac cggcgggacc attgccggtg
    11281 gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa agttggcgta gaaaatctgg
    11341 ttaatgcggt gccgcaacta aaagacattg cgaacgttaa aggcgagcag gtagtgaata
    11401 tcggctccca ggacatgaac gataatgtct ggctgacact ggcgaaaaaa attaacgccg
    11461 actgcgataa aaccgacggc ttcgtcatta cccacggtac cgacacgatg gaagaaactg
    11521 cttacttcct cgacctgacg gtgaaatgcg acaaaccggt ggtgatggtc ggcgcaatgc
    11581 gcccgtccac gtccatgagc gcagacggtc cattcaacct gtataacgcg gtagtgaccg
    11641 cagctgataa agcctccgct aatcgtggcg tgctggtggt gatgaacgac accgtactgg
    11701 acggtcgcga tgtcaccaaa accaacacca ccgacgtagc gaccttcaag tctgttaact
    11761 acggtcctct gggatacatt cacaacggta agattgatta ccaacgtacc ccggcacgta
    11821 agcacaccag cgatacgcca ttcgatgtct ctaagctgaa tgagctgccg aaagtcggca
    11881 tcgtttataa ctacgctaac gcatccgatc ttccggctaa agcactggta gatgcgggct
    11941 atgatggcat cgttagcgct ggtgtgggta atggtaacct gtataaatcc gtgttcgaca
    12001 ccctggcaac cgccgcgaaa aacggcactg cagtagtgcg ttcttcccgc gtaccgacgg
    12061 gtgctaccac tcaggatgct gaagtggatg atgcgaaata cggcttcgtc gcctctggca
    12121 cgctgaaccc gcaaaaagcg cgcgtcctgc tgcagctggc tctgacgcaa accaaagatc
    12181 cgcagcagat ccagcagatt ttcaatcagt actaatcacc tcgtcccggc atcgtgccgg
    12241 ggctttttca cttcagactt acgtccattg ccaattttca ttaccctaat gataatcacc
    12301 ggaataaatt attccgcgcg agggttttcg ggtgaaaaag caatggattg ttggcacggc
    12361 gctgcttatg ttgatgactg gtaatgcctg ggcagatggc gaaccgccaa ctgaaaatat
    12421 cttaaaagat caattcaaaa agcagtatca cggcattctc aagcttgatg ccatcagctt
    12481 aaaaaatctt gatgctaagg gtaatcaggc cacctggtca gcggaaggcg atgtctcttc
    12541 cagtgacgat ctctatacct gggtcggtca gttggcagat tacgaactgc tcgaacagac
    12601 ctggacgaaa gataaaccgg taaaattctc ggcgatgtta accagtaaag gaacgcccgc
    12661 gtctggctgg tcggtgaact tttactcttt tcaggcggca gccagcgatc gtgggcgggt
    12721 ggtcgacgat atcaaaacga ataataaata tctgatcgtg aatagcgaag atttcaatta
    12781 tcgctttagt cagcttgaat ctgcgttgaa tacccagaag aactcgattc ctgcgctgga
    12841 aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg cagaaagcag cggatgcata
    12901 ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa gaagccttta aaaaaattca
    12961 ccaacagcgt gatgagttta ataaacagaa cgatagcgag gcgttcgcgg ttaaatatga
    13021 caaagaggtt tatcaaccgg cgattgcggc atgtcataaa cagagtgaag agtgttatga
    13081 agtgccgatt cagcagaaac gagatttcga tatcaacgaa caacggcgac agacttttct
    13141 gcaatcacaa aaactcagcc gtaaattgca ggatgactgg gtaacgctcg aaaaagggca
    13201 atatccgtta acgatgaaag tttcagaaat aaacagtaaa aaagtcgcga ttttgatgaa
    13261 aattgacgat atcaaccaag ctaatgaacg ctagaaaaaa gataccgaac aactgcggcg
    13321 caatggggtg attaagtaac tcatgttaag tgcctttgcc ggataatata aaaagttatc
    13381 cggcaaatta ttttttacat tagccctagt agctgtggaa tgaacaagga tatttgcgga
    13441 atgtaagtga tcatcatcaa cgctactagc aatgcagcat aaaatagcag caatggtttg
    13501 attaaatgtt gaatcttcac acccgagata gaacaaccaa caaatagcgc actacccacc
    13561 ggcggtgtta acagcccaat acaaaggttg gccaccatca taataccgaa atgtactggg
    13621 tccattccca gctcttgtgc aatcggcaag aagatcggtg taaagattag tacagccggt
    13681 gtcatgtcca taaagatgcc gacaattaat aagacgatat taatcagcag caatatgatc
    13741 agtgggttat cagaaatccc catcaatgcg tcgctgatca tataaggaat atcagcatta
    13801 gtcattgccc aggacatgcc cacggaaaaa ccgatcaaca gtaatacaat cgaagtggta
    13861 acaaccgact ccagaatcag tttgggtaaa tggcgccatt ttacttcccg gtaaatcagc
    13921 accgagagaa taaatgtgta gacaacggca attgctgacg cttcagttgc ggtaaaaatg
    13981 ccgcccagaa tgccgcccat cacaatgaac accagaagca aacttggtaa ggcatccaga
    14041 aacgctttac aggcctgagc aaaggttgct ctttctgaaa gcgggtagcc acggcgttta
    14101 gcaataatgc cgcagactat cattaccgca agacccatta agatccctgg caaatatccc
    14161 gccataaaca gcgatgctac agaaacgccg ccagctgtca gggaaaacac aattaacacg
    14221 tttgagggcg gtatcagtaa tccagtaata caagatgaga cgtttacagc ggtagaaaat
    14281 gccggatcat aaccttcttt ggtctgaatg ggattcagtg tgccaccaac ggcagcagct
    14341 gccgcaacgg ctgaaccaga aatggaacca aacatcatat tcgccaggac gttcacatga
    14401 ccaagcgaac caggaacgcg gccaaccatt acctgggcga ggttaatcaa acgtatcgca
    14461 ataccgccgc tgttcatcag tgttccggca aagatgaaaa aggggattgc caacagggca
    14521 aagttatcca gtccattagc cagtcgttgc gaaacagtga ttgccgcgat atcccaggga
    14581 aacatcaaaa gcattgaagc tacagtggca ataccgattg agaacgaaat gggcacacca
    14641 ataaagacca gcacaaaaaa acagccaaac agtgtaatcg cgatataact atc
//
LOCUS       DAZPAB010000092        14653 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZPAB010000092 DAZPAB010000000
VERSION     DAZPAB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.092"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(407..694)
                     /locus_tag="ACHUPG_002465"
     CDS             complement(407..694)
                     /locus_tag="ACHUPG_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR0862051.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(769..912)
                     /gene="mgrB"
                     /locus_tag="ACHUPG_002466"
     CDS             complement(769..912)
                     /gene="mgrB"
                     /locus_tag="ACHUPG_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR0862052.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            1071..1310
                     /locus_tag="ACHUPG_002467"
     CDS             1071..1310
                     /locus_tag="ACHUPG_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR0862053.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            complement(1453..2244)
                     /gene="kdgR"
                     /locus_tag="ACHUPG_002468"
     CDS             complement(1453..2244)
                     /gene="kdgR"
                     /locus_tag="ACHUPG_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR0862054.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2421..3794
                     /locus_tag="ACHUPG_002469"
     CDS             2421..3794
                     /locus_tag="ACHUPG_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0862055.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            complement(3840..4721)
                     /gene="htpX"
                     /locus_tag="ACHUPG_002470"
     CDS             complement(3840..4721)
                     /gene="htpX"
                     /locus_tag="ACHUPG_002470"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR0862056.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMMMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4913..6961)
                     /gene="prc"
                     /locus_tag="ACHUPG_002471"
     CDS             complement(4913..6961)
                     /gene="prc"
                     /locus_tag="ACHUPG_002471"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR0862057.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVMLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6981..7679)
                     /gene="proQ"
                     /locus_tag="ACHUPG_002472"
     CDS             complement(6981..7679)
                     /gene="proQ"
                     /locus_tag="ACHUPG_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR0862058.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7776..8273)
                     /gene="msrC"
                     /locus_tag="ACHUPG_002473"
     CDS             complement(7776..8273)
                     /gene="msrC"
                     /locus_tag="ACHUPG_002473"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR0862059.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8403..9686
                     /gene="yebS"
                     /locus_tag="ACHUPG_002474"
     CDS             8403..9686
                     /gene="yebS"
                     /locus_tag="ACHUPG_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR0862060.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9655..12275
                     /gene="yebT"
                     /locus_tag="ACHUPG_002475"
                     /pseudo
     CDS             9655..12275
                     /gene="yebT"
                     /locus_tag="ACHUPG_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            12355..13794
                     /gene="rsmF"
                     /locus_tag="ACHUPG_002476"
                     /pseudo
     CDS             12355..13794
                     /gene="rsmF"
                     /locus_tag="ACHUPG_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            13912..14148
                     /locus_tag="ACHUPG_002477"
     CDS             13912..14148
                     /locus_tag="ACHUPG_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFR0862061.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            14253..14444
                     /locus_tag="ACHUPG_002478"
     CDS             14253..14444
                     /locus_tag="ACHUPG_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR0862062.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(14469..>14653)
                     /locus_tag="ACHUPG_002479"
     CDS             complement(14469..>14653)
                     /locus_tag="ACHUPG_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR0862063.1"
                     /translation="EWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHRV
                     DIGNLHYIDTGAVFGG"
BASE COUNT         3518 a         3734 c         3551 g         3850 t
ORIGIN      
        1 gcgtgcaata tcacattttc tgctatgcaa tgaataaaaa gttatatcac tttttctcat
       61 aaaacagtca gttaacggct atcaattacc ctaaagagaa gtcaatcccc aaagggattg
      121 taaatttaaa ataagaaaaa ttgatgagtg agcaaaaaaa aatcaagaga gaaacgtttc
      181 gctaataaat aataccacgc ttaatcacaa atctgatgta aacatcctaa taaattaatg
      241 aggaaaatat ctttttctga tatcccgtta cgcaaataaa taatatgata attatttgaa
      301 gtcagaccag agttttaatt tgaagcagtt gcaccatgac agtgcgtata atcaggatga
      361 attgagtaac ccttcaacga cggggttact caagattgag gaaggattat cgttcggcga
      421 ccaggcgaat aatatcttcg tcatcaaccg atttttgact ctcaatggtc tttttatctg
      481 ctttctctgg ttcgtttgct tcgcacaaac gacgtaacag tgcattttga cgtttttgct
      541 gatccagcaa cgcctcaagc agttcaatct gttcgttagt ccgtgaactg gcacgattga
      601 taaaaaacca caagatgagc ccgataagaa gaaccaccac cgatacaacc aaagacgcaa
      661 tgttcatcac gcctgaattt acaacttcgt tcatttcacc acctctgagt agaggcgcta
      721 ttctaccact gctggagagg aagaaaatct agtgctgaaa aaatgatatc accacgggat
      781 aaactggtta atggcacaaa ttccgctgaa aaattgtaca tcctgatcgc acatcatgtt
      841 gaatacctgc gcccaaagca gcaagcaagc caacaccacg acaaccagaa cgacccatcg
      901 aaactttttc actccaatct ccgcttcacc taccttatgt cattaaacta gcatgcgtta
      961 gttaaacagc gcctaactat gtcgatattc gtgctttttc ggaatgagta gcttgcttca
     1021 gcgcatattg ccgctacgat taagcgaaca taaaaagaga agagattgta atgcgattca
     1081 tcattcgcac agttatgctg atagcactgg tatggatagg attattactc agcggatatg
     1141 gcgttttgat tggaagcaaa gagaatgccg caggattagg tttgcaatgt acctatctga
     1201 ctgccagagg caccagcacg gtacaatact tgcacactaa aagtggtttc ttggagataa
     1261 cagattgccc actgttacgt aaaagcaata tcgtggtcga taatggttaa gggtaataaa
     1321 taaccagatt ctcactcatg agtaaaatat tcttatagca agcgcgttta atataacgca
     1381 ccttcaactt tatttttctc atgctaaata aagaacctgt ctgtttgata ttccgacagg
     1441 ttcttactct catcagaacg gatagtcgtg ataacccatt tgggcagaaa ttttgcgcgc
     1501 tgcggtatgc aacattgcga catattcctg taaacgctct tcagagaaac gcaacgtcgg
     1561 gaaggagatg ctcaaaccgg caatgaccac gccaaagcga tcaaataccg gtaccgcaat
     1621 gcattgcagc ccttcttcct gctcttcgtt atcttctcca tacccctgct cgcgcacctg
     1681 gtccagaacg ggtaataacg cttctgtact ggtgatggtc cgctcggtac tgcgtttata
     1741 ctccacgccc tcaagaattt gcttcacttc atcgcgatcg cgccatgcca gcagtacctt
     1801 accaatcgcg gtgctgtaca gcggattacg acgcccaatc cgtgaataca tgcgcaaatt
     1861 gtacatagag tcaattttgt gaatgtaaac aatactgtct tcgtccagtg cgccgaggtg
     1921 gatagtttct ttggtcaggc gggagatctc acgcatctgg atatctgcgc tacgaattaa
     1981 atcgacgttt tgtaacgcgc gagcgcccag ttcaaacaat ttcagggtca gggaatattt
     2041 ctccgactcc ccttcctgcg ccacataacc taaggttttc atggtctgta aaaagcgata
     2101 aacggtgctt tttgacatca tgacgcgctg cgacagctcg gttatcccta tttcgcgctc
     2161 ttcacccagc gcctgcaaaa tgccaaaaac ttttagcacg gaagatacag aatcaggctg
     2221 tttatccaga tctgcgttag ccatttatca cctcattgcg agtgttttat aaaaatcaga
     2281 actgtttttt attataattt cgcaccaggg tggtcgcaat ccatcttttg ccggttagtt
     2341 acaattctgc gacatccacc gtgaatatca gtgctagaat catacccctg ttgattattc
     2401 accaaagata taaaattcct atgccaaaag ttcaggccga cggcctgcca ttgccccagc
     2461 gatacggtgc gatattaacc attgtgattg gtatttcgat ggctgtcctt gacggcgcaa
     2521 tcgccaacgt cgccctgcca acaattgcca cggaccttca tgccacacca gccagttcca
     2581 tctgggtagt gaacgcctat caaatcgcca ttgtcatctc cctgctctcg ttttcgtttc
     2641 tgggcgatat gtttggctat cgacgtattt ataaatgcgg tctggtcgtt tttctgttgt
     2701 cttcactgtt ctgcgccctt tctgattcgc tgcaaatgct cacccttgcg cgtgtcatac
     2761 aaggtttcgg cggtgcagcg ttgatgagcg ttaataccgc acttatccgc ctgatctatc
     2821 cacaacgttt tctggggaga gggatgggca taaactcgtt tattgttgcc gtctcttctg
     2881 ctgccgggcc gacaattgct gcagcaatcc tctccatcgc atcctggaaa tggttatttt
     2941 taatcaacgt accgttaggt attatcgccc tgcttctggc gatgcgtttt ctgccaccca
     3001 atggttctcg cgccagtaaa ccccgtttcg acctgcccag cgccgtgatg aacgcgttaa
     3061 ccttcggcct gcttatcact gcgttgagtg gtttcgctca ggggcaatcg ctgacgttaa
     3121 ttgctgcgga actggtggta atggttgttg ttggtatttt ctttattcgc cgccagcttt
     3181 ctcttcccgt accgctgcta ccggtggatt tactgcgtat cccgctgttt tcactttcta
     3241 tttgcacatc tgtttgctct ttctgcgcac aaatgctggc aatggtttcc ctgccctttt
     3301 acctgcaaac cgtgctcggg cgtagtgaag tcgaaacagg tttacttctg acaccgtggc
     3361 cgttagcaac gatggtgatg gctcctctgg caggctattt gattgaacgc gtacatgcag
     3421 gattgctggg tgctttaggg ttgttcatca tggctacggg gcttttttcc ctggttctgc
     3481 tgcccgcgtc acctgcggat atcaatatta tctggccgat gatcttatgt ggcgctggat
     3541 ttggcttatt ccagtcaccc aataaccaca ccattattac ctccgctccg cgcgaacgta
     3601 gcggtggggc cagtggcatg ttaggaacgg ctcgtctact gggtcagagt agtggcgcgg
     3661 cgctggtggc gctgatgcta aatcagtttg gtgataatgg tacgcacgtc tcgctgatgg
     3721 ctgcggctat tctggcagtg attgctgcct gtgtcagtgg tttacgtatc actcagccac
     3781 gagccagggc ataaaaaaag cgcgtcgatc aggacgcgct ttttagtatt tacttcatat
     3841 tacttcaggt attcacccgt acgcagagct tcaatacgtt tatccagcgg cgggtgagtc
     3901 atgaacaact cactgagcga tttcgactta ccgttaatgc agaaagccat catgctggtt
     3961 gcttcttgcg gttcatagct ggttttcagg cgctgcagcg cggcaatcat tttctcgcga
     4021 ccaaccagtt ttgccgaacc ggcatctgca tggaattcac gatgacgcga gaaccacatg
     4081 gtgataatgc tcgccagaat accaaacacc agttccagaa ccgttgcaac cgcaaagtag
     4141 atcagcgggt tgccgttgct ctcttcacct tcatcacgat ttccgcccat aaaacccgcg
     4201 gcaagctgcg ccagaatacg ggaaataaag ataacgaagg tgttcaccac gccctgaatc
     4261 agcgtcatgg tgaccatatc accattggcg atgtggctga tttcgtgagc aattaccgcc
     4321 tcggcttcat ccgggctcat gttctgcagc aaaccggtgc tgacagcaac cagagaggca
     4381 tcacggcgcg caccggttgc aaaagcgttg atgtccggcg catggtagat agccacttgc
     4441 ggcatagcga tccccgcctg acgagcctgg gttgctacag tattgaccag ccaacgttcc
     4501 ctttcgttac gcggttgctc gatcacttcc ccgccaacag atcgtaatgc catccatttg
     4561 gacatcagaa gcgaaacgaa ggaaccacca aaaccgaaca gcaaggccat catcatcagc
     4621 ccctgaacgc tgctcgactg tatccctgtc aggctcagta ccagcccgaa aacgaccatt
     4681 acggccaggt tcgttagcag gaagagcgcg attcgcatca taattttctt tttacctcag
     4741 tttaacaaaa cgcaatatgc gatacccaca tcgtatgggt tacgcgacta ttttcaagtc
     4801 tggatagtgc gtaagtcacc agaaagacac aactttacat tttgtagcat ctgatttacg
     4861 gcatcttgcc gctgttaaaa aatcaggcac aatttcttgt gcctgattga tattacttga
     4921 cgggagcggg ttgttccgcg ggtctggctt tttcaagctt cgccagatcg agtgcgatat
     4981 tcaccgtctc atccagataa ggatccggct cctggtaatc tttcgataga tcatccagct
     5041 tcttcaactc cggtttacct tctcgtttaa agcgttcgtt caaacgcgcc agacgcgtcg
     5101 catcatcttc attattctct ttctcacgca cagcgtagtt cagagaaacg atattgcgct
     5161 tgtccttcat agcgttgaag cgcgcgatat ccttcatgat gttctggaac tcaggatctt
     5221 tcgcgatacg cgcattatgt tccttcagca gctccggttc aaaggcagtt aaatctcctg
     5281 atttcacata agtcgcggca tcgatgctat cccacggcag cgcgttatct tcgaatttct
     5341 cacccgtttc cgtttcttca ttacccgtcg gcatgatgat gtctggtgtt acgcctttac
     5401 gttgcgtact gccgccgtta acgcgataga atttctggat cgtgtactgc acagaaccca
     5461 gcgctggcca ttcaggacgt aacatctgat cgtaaatacg gttcaatgaa cggtattgct
     5521 gaacggtgcc tttaccaaac gtcggttcac ccacaaccag cgcacgaccg taatcctgca
     5581 ttgccgcggc aaagatttct gaagccgaag cactgaaacg gtcaaccagt accaccagcg
     5641 ggcctttata gaacacctgc ccgtcggtat cgctgtcttc acgaaccttg ccgttgttat
     5701 cgcggacctg aacaatggga cccgcaggaa taaacagacc tgagagcgat accgcttcgg
     5761 tcaacgcccc accgccattg ctacgcaggt cgatgatcac actgctgaca ttctgttttt
     5821 ccagtttctg cagttgaact ttgacatcgt ctgtcaaacc cacatagaag cccggaatat
     5881 ccagcacgcc gactttctct ttaccgacgg tcttcaccga cattttaacc gcgcggtctt
     5941 cgagacgaat acgttcacgg gtcaacatta cagtacgggt cttggtccct ttaccagcag
     6001 gtaaaatttc cagacgaact ttactgccct tcggcccttt aattaaggca accacatcat
     6061 caagacgcca gccaatcacg tcaaccatcg gcttgcctgt ttgaccaaca ccgacaattt
     6121 tgtcaccaac gctgatagct ttactcttcg ctgccggacc acctgccacc atcgaattga
     6181 taacggtgta gtcatcatcc atttgcagca ctgcgccaat accttccagt gacaaactca
     6241 tttcagtgtt gaactgttcg gtattacgcg gggaaagata gttggtatgc ggatcgattt
     6301 cacgcgcaaa cgccgtcatt gccagcgaga aaacatcttc gctgttggtt tgcgccagac
     6361 gacgaatggc aaatttgtag cggcgtgtca gggtttcacg aatttcttta tccgtttttc
     6421 ctgtcagctt caggcttaac tcgtcgaatt tgactttact gtcccactgc gcgttcaact
     6481 cagcctcgtt tttcggccag ggcgctttgc tgcggtcaag gttataagtg tcgttgccgg
     6541 tgaaatccat cggcttttcc agtaccgaca aagcgtactg gtaacgttca aaacggcgct
     6601 tttgcgccag attgtagaga tcgtagaaaa cgtcgagttt gcctgaacgc agttcatcgc
     6661 ctaactcggt tttctttttc gcgaactgtt caacatcgct tgccagcagc acattgtggc
     6721 tgtaatcgag cagattcagg tagcggtcaa agattttggc cgaaaatgcc tgatcgaggt
     6781 cgaactggcg ataatgagaa cgggtgaagc gcgacgttac gcgctcactt accgtcgcat
     6841 gctgcgtctc ttcctttaat accggaattt gatcagcacg cgtgatatct tctacagcga
     6901 aggtctggcc tgctattgca agcaggccag ctaacgcggt aagcctaaaa aacatgttca
     6961 tgcctggccc ggcctccgtt tcagaacacc aggtgttctg cgcgcacaat caaagacata
     7021 cccgaattca gctggacgcg gacgccgtct ttggtgattt ctaatacggt ggcatccatc
     7081 gcgttttgac ccgctttcac cttcagggct tgtccgacag tcagagctga aatgtcagaa
     7141 accggggtgt gctgttcttc gcgaggtgct tttactgttt ttggcgcttt ctctaccggc
     7201 ttttgcgcac gaggtttacg ttcagcgcct tctttgcggc gtggcgtggt cggacgtggc
     7261 ttgcgttcgc ggcgcggtgc gtcttctttc tcaccagcag ctgcggcagc ttcgcgtttt
     7321 ttcgcttgct gttcagcacg ctgtgcctga acacgcgctt tcgcttcttc aagctgcttg
     7381 cgagcatgct ctacatgttg ctcgtccagc tcaccgcatg ggttgccgtc aagatcgaca
     7441 cgcgttgcgc cgggtttaac accgtaaaga taacgccagc tcgaagtgta gagacgtaaa
     7501 gcggatcgta attgcgtttt gctcaggttc atttccccgg caacacgatc gaccaaatcc
     7561 tgaaaaatac caattttcag cggacgcgct tcaccttccg cactgaaaca gtggggaaaa
     7621 cgttcggcca gaaacgcgat tacttcttta ctgctattca acttaggttg attttccatg
     7681 aaatttcctg attacaacgg acgtagccaa caagcgcagg catgaacagg cgtcattata
     7741 atgacgctac cagtaaatgc tacgttatcc gttgattatc ctgcgacgct cgcaaagaat
     7801 tttttgtaat ccgtcgttgc aagcactttt tcaagctgtg ccacaagctg acgtaagcct
     7861 tgctcgtcct cgtctgtaaa gcgagcgaag acagtactat cgatgtcgag aacaccaata
     7921 atctgatttt tcaccaccag cggcagaaca atttcagaat tactcgccgc atcacaggca
     7981 atatgcccgt caaacgcgtg aacatcctcg atacgctgca cttgattgcg ggcaaccgct
     8041 gtgccgcaca ccccgcgccc gacgggtatc cggacacagg caattttgcc ctgaaatggt
     8101 ccgagtacca gtgtatcgtc ctcaagcaaa taaaaacctg cccagttaac gtcagtgaga
     8161 cgctcgtata acaacgcact ggtgttcgca agcgttgcca gaaaactggt ttctcccgcc
     8221 atcagcgcgt taaagtcgcg atttaaatcc gcgtaaaatt ctgttttgtt cattacttaa
     8281 tcacttggtt gtcttacctg gaactgccag cctattaaaa taagcattaa atgcgttaat
     8341 gctcaagatc attcccatca tgggttaaga ttaatgttaa ttcttattac atttggcacg
     8401 tcatggctct taacacacca caaattacgc cgacaaaaaa gataacagtg agggcaatcg
     8461 gcgaggaact gccgcgtggt gattaccaac gttgcccgca atgtgacatg ctgtttagcc
     8521 tgcccgagat aaattctcat caaagtgcct attgtccgcg ctgtcaggca aaaattcgcg
     8581 acgggcgcga ctggtcgcta acgcgcctgg cggcaatggc cttcaccatg ctgttgctta
     8641 tgccgtttgc ctggggcgaa ccgctgttgc atatctggct gttaggcatt cgtattgacg
     8701 ccaacgtcat gcaaggcatc tggcaaatga ccaaacaggg cgatgcgata acgggatcaa
     8761 tggtcttttt ctgcgttatt ggtgcccccc tcattctggt gtcctccata gcatatttat
     8821 ggtttggtaa ccgactggga atgaatttac gtccggtact gctgatgctt gagcgactta
     8881 aagagtgggt aatgctggat atctacctgg tcggcattgg cgttgcttct ataaaggtac
     8941 aggattatgc ccatatccag gcgggtgtcg gcttgttctc ttttgtggcg ttggtgattt
     9001 taacgacggt gacgttgtca catcttaatg ttgaggagct gtgggagcga tattatccgc
     9061 agcgccccgc tacgcgtagg gacgagaaac ttcgtgtctg tcttgggtgc cattttaccg
     9121 gctatcctga tcagcgtggt ctctgcccgc gttgccatat cccgctacgc ctgcgtcgtc
     9181 gtcatagttt gcaaaaatgc tgggcggcgc tgttagcgtc aatcgttttg ttgttaccag
     9241 ccaacctatt gcctatttct atcatttatc tgaatggtgg ccggcaggaa gatacgattc
     9301 tttccggaat tatgtcgctg gcaagtagca acattgcggt tgcgggaatc gtgtttatcg
     9361 ccagtattct ggtaccgttt actaaagtga tcgtcatgtt cactttactg ttgagcattc
     9421 attttaaatg ccagcaaggt ttacgcacac gcattctgtt actgcggatg gtgacctgga
     9481 ttggacgctg gtcgatactc gacctgtttg tcatatcttt aaccatgtcg ctgattaatc
     9541 gcgatcagat cctcgctttt actatgggac cggctgcgtt ttattttggc gctgccgtaa
     9601 ttttgactat tcttgctgtg gaatggctgg acagccgctt actttgggat gcacatgagt
     9661 caggaaacgc ccgcttcgac gactgaagcg cagattaaaa ataaacgccg tatctcacct
     9721 ttctggctgc tgcctttcat cgcgctaatg attgccggtt ggctgatttg ggacagttat
     9781 caggaccggg gtaataccgt caccatcgac tttatgtcgg cggatggtat tgttccgggc
     9841 cgtacgcctg ttcgttatca gggcgttgaa gtcggaacag tgcaggatat cagcctcagc
     9901 gacgatcttc gtaagattga agtcaaggtc agcatcaagt ccgatatgaa agatgcgctg
     9961 cgcgaagaga ctcagttctg gctgatgacg ccaaaagcat cgttggcagg tgtctccggg
    10021 ctggacgccc tcgtcggtgg gaactatatc ggcatgatgc cgggtaaagg taaagagcag
    10081 gatcactttg tcgcgctcga tacccaaccg aaatatcggc tggacaatgg cgatctgatg
    10141 atccacctgc aagcccccgc tctcggttcg ctgaacagcg gttcattggt ctatttccgc
    10201 aagatcccgg tgggaaaagt ctacgactat gccatcaatc ccaacaagca aggcgtggtg
    10261 attgatgtcc tgatcgagcg gcgttttacc gatctggtga aaaaaggtag ccgtttctga
    10321 aacgtttccg gcgttgatgc caacgtcagt atcagtggcg cgaaggtgaa actggaaagt
    10381 ctggcggcac tggttaacgg tgcgattgcc ttcgattcac ctgaagagtc gaaacctgcc
    10441 gaggcggaag atacctttgg tctgtatgaa gatctggccc acagccagcg tggagtaata
    10501 ataaaactgg aactgccgag tggagcagga ttaaccgccg actcgacgcc gttaatgtat
    10561 caggggctgg aagtcggaca gctgactaaa ctggatttaa atcctggtgg taaagtcact
    10621 ggggaaatga ccgttgatcc ctgcttcgtg aaaatacccg catcgaatta cgcaacccga
    10681 aattatccct tagcgatgct aatctcagcg ccctgctgac cggaaaaacc tttgagttgg
    10741 tgcccggcga tggcgagcca cgcaaagagt tcgttgttgt gccgggcgaa aaagccctgc
    10801 tgcaagagcc ggacgttctg acactgaccc tgacagcacc ggaaagttac ggtattgatg
    10861 cgggtcagcc gctcattctt cacggcgtgc aggtaggcca ggttatcgat cgtaaactca
    10921 ccagcaaagg cgtcaccttt accgtcgcca tcgagcctca gcatcgagaa ctggtaaaag
    10981 gcgatagcaa atttgtcgtc aacagccgtg tcgacgtgaa ggtggggctg gatggcgttg
    11041 agtttctcgg tgccagcgcc tcagaatgga ttaacggcgg gatacgtatt ctgccgggcg
    11101 ataaaggcga gatgaaagcc agctatccac tgtatgccaa tctggaaaaa gcgctggaga
    11161 acagccttag cgatttaccc accacaacca tgagtttgag tgcagagacg ctgccggatg
    11221 tgcaggcagg atcggtagtg ctgtaccgta aatttgaagt tggtgaagtt attaccgtgc
    11281 gtccgcgagc taacgcgttc gatatcgatc tgcacattaa accggaatat cgcaaccttt
    11341 tgaccagcaa cagcgtgttc tgggcagaag gcggggcgaa agttcagctg aatggtagtg
    11401 gcctgaccgt acaggcatcc ccgctctcca gagcattaaa gggggccatt agcttcgata
    11461 acctcagtgg tgccagcgcc agtcagcgta aaggcgacaa gcgaattctg tatgcttccg
    11521 aaacagcggc ccgtgcggtt ggcaggcaga ttacgcttca cgctttcgat gccggaaaac
    11581 tggcggtcgg gatgccaatt cgctatctcg gtattgatat cgggcaaatc cagacgctgg
    11641 atctgattac cgcacgcaat gaagtacagg caaaggcggt gctctatccg gaatatgtcc
    11701 agacctttgc tcgcggtggt acgcgcttct cagtggtcac accgcaaatc tcggcagctg
    11761 gcgttgagca tctagatact atcctccagc cgtatatcaa cgtcgaacca ggccgtggca
    11821 atcctcgtcg cgactttgaa ttagaagagg ccaccattac tgattcgcgt tacctagatg
    11881 gtttaagcat tattgttgaa gcgccggaag ccggttcgtt aggcatcggt acgcctgtgc
    11941 tgttccgtgg tctggaagta ggtacggtta ccggaatgac gctggggaca ttgtcagatc
    12001 gcgtgatgat tgcgatgcgc atcagtaaac gctatcaaca cctggtgcgt aacaattccg
    12061 tcttctggtt ggcatcgggt tacagtctgg actttggtct gacgggcggc gtagtgaaaa
    12121 ccggcacctt taaccaattt atccgtggcg gcatcgcctt cgccacgcct ccgggtacgc
    12181 cacttgcacc gaaagcccaa gaaggcaaac acttcctgtt gcaggaaagt gaaccgaaag
    12241 agtggcgtga atggggaact gcgcttccca aataatgccc actgctccgg cgtgcctgcg
    12301 ccggagcgct tatgctaaac tgcgcgcctg tttttttgcc agtggtacat gctcgtggcc
    12361 caacacaccg tttatttccc ggacgcgttt ctgacacaaa tgcgcgaagc tatgccttcg
    12421 acgctctcat ttgatgattt tcttgccgcc tgtcagcgcc cgttgcgccg cagcattcgc
    12481 gttaatacgc tgaaaatctc cgttgctgat ttcctgcaat taaccgctcc ttatggctgg
    12541 acgcttacgc caattccgtg gtgtgaagaa ggtttctgga ttgaacgcga caatgaagat
    12601 gcattgccat tgggtagtac caccgagcat ttaagtggcc tgttttatat tcaggaagcc
    12661 agttcaatgt tgcccgtcgc cgccttgttt gctgacggta atgcaccaca gcgggtgatg
    12721 gatgtcgctg ctgcgccagg ctcaaaaacg acgcaaattg ccgcgcggat gaataacgaa
    12781 ggggcaatcc ttgccaatga gttttccgcc agttgggtaa aagtgttaca tgccaatatc
    12841 agccgctgtg gcatcagtaa tgttgcgctc acacattttg gtggccgcgt gtttggtgcg
    12901 gcagtgccag aaatgtttga tgccattttg ctggacgctc cctgctccgg cgaaggcgtg
    12961 gtgcgtaaag atcccgatgc gctaaaaaac tggtcacccg aaagcaatca ggaaatcgca
    13021 gctacccaac gggaactgat tgacagcgcc tttcatgcat tacgtcctgg cggtacgctg
    13081 gtttactcga cctatacctt aaaccgggaa gaaaacgaag ccgtttgccg gtggctgaaa
    13141 gagacttacc ccgacgaagt agagttttta ccacttggcg atctcttccc tggtgcaaac
    13201 aaggcgctga ccgaagaagg ttttttgcat gttttcccac aaatttacga ctgcgaaggc
    13261 ttcttcgttg ctcgtctgcg taaaactcag gcgatccccg ccttacccgc cccaaaatac
    13321 aaagtgggca atttcccgtt cagcccggtg aaagatcgcg aagccggaca aattcgtcag
    13381 gcggctgcaa gtgttggctt aaactgggat ggaaacctgc gactctggca acgcgacaaa
    13441 gaactgtggt tgttcccggt aggcattgaa gccctgatcg gtaaagtccg attttctcgc
    13501 ttagggatta aacttgccga aacgcataac aaaggttatc gctggcagca tgaagcggtt
    13561 attgcccatg cctcccccga caatgtaaac gcttttgaac tgacaccgca ggaagcgtag
    13621 gagtggtatc gcgggcgcga tgtttacccg caagccgcgc cagtggcgga tgacgtgttg
    13681 gttactttcc agcatcagcc gattggttta gccaaacgga ttggttcgcg actgaaaaac
    13741 agctacccgc gtgaactggt gcgggacggg aaacttttta ccagtaacgc atgacagcgc
    13801 acaaaaaaag cgcacttttt gactggcaca ttcgccggcc tcaactaggc tgaaaaattg
    13861 tgcgatcgga ctggtcgtac tacaatcagc agctaaatgg agagcacgaa gatgaaaacc
    13921 agtgtgcgca taggcgcttt tgaaatcgac gacggcgaat tacacggtga atcgccgggt
    13981 gatcgaacgt taaccattcc ttgtaaatct gaccccgatt tatgtatgca actggatgcc
    14041 tgggatgctg aaaccagtat ccctgccctg ctaaatggcg aacactctgt cctttaccgt
    14101 acccgttacg atcaacaatc tgatgcctgg attatgcgtc ttgcctgatc caaaaagaac
    14161 ccgtcggcat ggcgggttat ttgtcctggt tattcccccg ttgtaaaatc tctcctaaac
    14221 ttaacggtac ggcaccacac ttcggggatg aaatgttcgc gctggtactt tttgtttgct
    14281 acctggatgg cggttgtgaa gatattgttg tggatgtcta caacacggaa cagcagtgtc
    14341 tttattctat gagcgatcaa cggatccgcc atggcagttg ttttccgatt gaggatttta
    14401 tagatggttt ctggcaacca gcacaggagt atggtgattt ttaattattg cagttgcaca
    14461 agagtcagtt agcccccaaa gacagcaccg gtatcaatat aatgcaggtt gccaatatcc
    14521 acgcgatgtc gcaacggtgt atgaccaaac cagaaatgat cagcacctgt aattccctgc
    14581 ccttttgggc gttcacctaa tcgcgagcgg ctccacaaga cctgatgcaa atcaacgtcc
    14641 ttttgccatt cat
//
LOCUS       DAZPAB010000093        14481 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZPAB010000093 DAZPAB010000000
VERSION     DAZPAB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.093"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..118
                     /locus_tag="ACHUPG_002480"
     CDS             <1..118
                     /locus_tag="ACHUPG_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862064.1"
                     /translation="NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(115..381)
                     /locus_tag="ACHUPG_002481"
     CDS             complement(115..381)
                     /locus_tag="ACHUPG_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR0862065.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            655..4179
                     /gene="nifJ"
                     /locus_tag="ACHUPG_002482"
     CDS             655..4179
                     /gene="nifJ"
                     /locus_tag="ACHUPG_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR0862066.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4546..5675
                     /gene="ompN"
                     /locus_tag="ACHUPG_002483"
                     /pseudo
     CDS             4546..5675
                     /gene="ompN"
                     /locus_tag="ACHUPG_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5816..6250
                     /gene="uspF"
                     /locus_tag="ACHUPG_002484"
     CDS             5816..6250
                     /gene="uspF"
                     /locus_tag="ACHUPG_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR0862067.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6426..7361
                     /gene="ttcA"
                     /locus_tag="ACHUPG_002485"
     CDS             6426..7361
                     /gene="ttcA"
                     /locus_tag="ACHUPG_002485"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR0862068.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7490..8857)
                     /gene="dbpA"
                     /locus_tag="ACHUPG_002486"
     CDS             complement(7490..8857)
                     /gene="dbpA"
                     /locus_tag="ACHUPG_002486"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR0862069.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(8887..9060)
                     /gene="ynaL"
                     /locus_tag="ACHUPG_002487"
     CDS             complement(8887..9060)
                     /gene="ynaL"
                     /locus_tag="ACHUPG_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR0862070.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(9335..10318)
                     /gene="zntB"
                     /locus_tag="ACHUPG_002488"
     CDS             complement(9335..10318)
                     /gene="zntB"
                     /locus_tag="ACHUPG_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR0862071.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(10332..10379)
                     /gene="ydaN"
                     /locus_tag="ACHUPG_002489"
     CDS             complement(10332..10379)
                     /gene="ydaN"
                     /locus_tag="ACHUPG_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR0862072.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            10573..11805
                     /gene="dgcM"
                     /locus_tag="ACHUPG_002490"
                     /pseudo
     CDS             10573..11805
                     /gene="dgcM"
                     /locus_tag="ACHUPG_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11826..12389)
                     /gene="smrA"
                     /locus_tag="ACHUPG_002491"
                     /pseudo
     CDS             complement(11826..12389)
                     /gene="smrA"
                     /locus_tag="ACHUPG_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12643..14097)
                     /locus_tag="ACHUPG_002492"
     CDS             complement(12643..14097)
                     /locus_tag="ACHUPG_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HFR0862073.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14094..>14481)
                     /locus_tag="ACHUPG_002493"
     CDS             complement(14094..>14481)
                     /locus_tag="ACHUPG_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR0862074.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3861 a         3651 c         3418 g         3551 t
ORIGIN      
        1 taacaccatt agcgaaatgc tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt
       61 aacgctggcg accgcgaaac agacattaac ttataagctg gcggatttaa tgaattaata
      121 gctgccacag ctcccggcgg caagtgactg ttcgctacag cgtttgccgt tgggtaatgc
      181 acacatccca atcgccgtac catccagttg acgggcaaca gaaagcgaac cgccgatcat
      241 tgcacaattt gcttctccac tactggacat cgacgctttt aaacttggcg ctacgtgcgc
      301 cgcagtcgcc tgctgaacag gctcactact acacgccgac aacaataaag cggcacaccc
      361 tacccaaaac gctgctcgca tctcttcttc ctctgatctt caagccaaac gacaccgcca
      421 taaataatag gcagcacaga gggcggcgtc gagagctgtc ctgcgcgttg ccccgcattt
      481 ttactttttt atggctattt ttttgccctc tgtttgatca aaacattcat tactctgatg
      541 tgggggacac aaaagcgaaa atgcagaaga aagccatttg ctaaaattga aggattacta
      601 ctgggcgcgc agcaatttcg tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt
      661 actattgacg gtaatggcgc ggttgcttcg gtgacgtttc gcatcagtga agttatcgcc
      721 atctacccta ttacccccag ttccacgatg gcagaacagg ctgatgcctg ggccggaaac
      781 ggcttgaaga acgtttgggg agacacacca cgcgtggttg aaatgcagtc ggaagcgggt
      841 gctatcgcta ccgtgcatgg cgctttgcag acgggtgccc tttcaacatc gtttacgtca
      901 tcgcagggtt tgctgctgat gatcccgacg ctgtacaaac tggcaggcga actgacgcca
      961 tttgtcctgc atgtagcggc acgtaccgtt gccacacatg cactttctat ttttggcgat
     1021 cattccgacg ttatggcggt gcgccagacg ggttgcgcga tgttgtgtgc ggctaacgtc
     1081 caggaagcgc aagactttgc tctcatttcg cacatcgcaa cgctgaagag ccgcgtgcca
     1141 tttattcatt tctttgatgg ttttcgcacg tcccacgaaa tcaataaaat tgtcccgctg
     1201 gccgatgaca ctattcttga tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg
     1261 gcgctcaacc cggaacatcc ggtgatccgc ggtacgtccg ccaatcctga cacttatttc
     1321 cagtctcgcg aagccaccaa tccctggtac aacgcggtct atgaccatgt tgaacaggcg
     1381 atgaatgatt tcgctgccgc gacaggtcgt cagtatcagc cgtttgagta ttacgggcat
     1441 ccgcaagcgg aacgggtgat tatcctgatg ggctctgcca ttggcacctg tgaagaagtg
     1501 gttgatgaat tgctaacccg tggcgaaaaa gttggcgtgc tgaaagttcg tctgtaccgt
     1561 cccttctccg ccaaacactt gctgcaagct ctgccgggat ccgtacgcag cgtggcggta
     1621 ctggacagaa ccaaagaacc cggtgcccag gcagaaccgc tctatctgga tgtgatgacc
     1681 gcactggcag aagcctttaa taatggtgag cgcgaaaccc tgccccgtgt cattggtggg
     1741 cgctatggtc tttcatccaa agaatttggc ccggactgtg tactggcggt atttgccgag
     1801 ctcaacgcgg ctaaacctaa agcacgcttt acggttggta tttacgatga tgtgaccaat
     1861 ctgtcactgc cgttgccgga aaacactctg ccaaactcgg cgaaacttga agccttgttt
     1921 tatggccttg gtagtgatgg cagcgtttcc gcaaccaaaa acaatatcaa gattatcggt
     1981 aattccacgc cgtggtacgc acagggctat tttgtttacg actccaaaaa agcgggcggc
     2041 ctgacggttt ctcaccttcg cgtgagcgaa cagccgattc gttccgctta tctcatttcc
     2101 caggctgatt ttgttggctg ccaccagttg cagtttatcg ataaatatca gatggctgag
     2161 cgtttaaaac ctagcggcat tttcctgctt aatacgccgt acagcgcaga tgaagtgtgg
     2221 tcgcgcttgc cgcaggaagt tcaggccgtg ttaaaccaga aaaaagcgcg cttctatgtg
     2281 attaacgcgg cgaaaatcgc ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg
     2341 cagatggctt ttttccatct gacgcaaatt ctgcctggcg atagcgccct cgcagaattg
     2401 cagggtgcga ttgccaaaag ttacagtagc aaaggtcagg atctggtgga gcgcaactgg
     2461 caggctctgg cgctggcgcg tgaatccgta gaagaagttc cgttgcaacc ggtaaatccg
     2521 cacagcgcca atcgaccgcc agtggtttcc gatgccgccc ctgatttcgt gaaaaccgta
     2581 accgcggcga tgctcgccgg gcttggtgat gccctccccg tttcggcgct gccgccagac
     2641 ggcacctggc cgatgggcac cactcgctgg gaaaaacgca atatcgccga agagatcccc
     2701 atctggaaag aggaactctg tacccaatgt aaccactgcg tcgccgcttg cccacactca
     2761 gctattcgcg caaaagtggt accgcctgaa gcgatggaaa acgcccctgc cagcctgcat
     2821 tcgctagatg tgaaatcgcg tgatatgcgc gggcagaaat atgtcttgca ggtggcaccg
     2881 gaagattgca ccggttgtaa cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat
     2941 ccagagatta aagccatcaa tatgatgtct cgccaggaac atgtcgaaga agagaaaatc
     3001 aattacgatt tcttcctcaa cctgccagaa atcgaccgta gcaaactgga acgtattgat
     3061 attcgtacat cgcagctgat tacaccgctg tttgaatatt caggtgcttg ccccggttgt
     3121 ggcgagacgc cgtatattaa attgctgact cagctctatg gtgaccggat gttgatcgct
     3181 aacgccaccg gctgttcttc aatttatggc ggtaacctgc cctctacacc gtataccacc
     3241 gatgccaacg gtcgtgggcc ggcatgggcg aactctctat ttgaagataa tgccgaattt
     3301 ggccttggtt tccgcctgac ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat
     3361 caatttgccg ataaaatccc ggcggaatta ctgacggcgt tgaaatcaga cgctacgcca
     3421 gaggttcgtc gagaacaggt cgcagcttta cgccagcaac tcaacgatgt tgccgaagca
     3481 catgaacttc tgcgtgacgc agatgcactg gtggaaaaat caatctggct gattggtggc
     3541 gacggctggg cttacgatat cggctttggc ggtctggatc atgtattgag tttgacggaa
     3601 aacgtcaaca ttctggtgct ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg
     3661 aaagcgacac cgctgggtgc agtaactaag ttcggcgagc acggcaaacg taaagcgcgt
     3721 aaagatcttg gcgtcagtat gatgatgtac ggtcatgttt atgtggcgca gatttctctc
     3781 ggcgcgcagc tgaaccagac ggtgaaagcg attcaggaag cggaagcgta tccgggacca
     3841 tcgctgatca ttgcttatag cccgtgtgaa gagcatggtt acgatctggc tctcagccac
     3901 gaccagatgc gccaactcac tgctaccggc ttctggccgc tatatcgctt tgatccgcgt
     3961 cgtgccgatg aaggcaaact gccgctggcg ctggattcac gcccgccgtc agaagcactg
     4021 gaagaaacgt tacttcacga gcagcgtttc cgtcgtctga attcgcagca gccagaagtg
     4081 gcagaacagt tatggaaaga tgctgcagct aatttgcaaa aacgctatga cttcctagca
     4141 caaatggccg gaaaagcgga aaaaagcaac accgattaaa tgctctggat aaggattatc
     4201 caattctaaa aaaaaagccc ggacgactgt tcgggcttgt ctttttatat gttggaaaat
     4261 cagtggcaat gcaatggccc aacagaaaat aaaatctgtg acaataaagg catataaccc
     4321 gcgcagaata acgtatagga aattaaaagt ataattttta ttttgtatgt attatcttcg
     4381 atacattcac cagacttatt atcatttcag aggaattatt tgattaaggt tttacttaag
     4441 gcgtaacaaa tgatatttat cggctaactg aacttctcct ttaggatgtt ttcactcccc
     4501 gaaatgggga taaaaggcaa ataaaataac taaaggattt attcaatgaa aagaaaagta
     4561 ctggcacttt taattcctgc cctgctcgcc gcaggtgctg cacatgcagc cgaagtttat
     4621 aataaagacg gcaacaaatt agatctgtat ggcaaagttg atggcctgca ttatttttct
     4681 gataattcag cgaaagatgg cgaccagagc tatgcgcgtc tgggttttaa aggcgaaacc
     4741 caaattaacg atcaacttac tggctacggt caatgggaat acaatattca ggcaaacaac
     4801 actgaatctt caaaaaacca gtcatggacc cgtctggcat ttgccggact gaaatttgca
     4861 gattacggtt ctttcgatta cggacgtaat tatggcgtaa tgtacgacat cgaaggctgg
     4921 accgatatgc tgcctgaatt tggcggtgac tcttatacca atgcagacaa ctttatgact
     4981 ggtcgagcca atggcgtcgc gacttatcgt aatactgatt tcttcggtct ggtaaatggt
     5041 ctgaacttcg cggtgcagta tcaaggtaac aacgaaggag ccagtaatgg tcaggaaggc
     5101 accaataacg aacgcgatgt tcgtcatgaa aacggtgacg gctggggtct ttccacaaca
     5161 tatgatttag gcatgggctt tagcgctggt gcggcataca cctcttctga ccgcaccaat
     5221 gaccaggtta accatactgc ggcgggtggt gataaagcag acgcgtggac tgctgggcta
     5281 aaatacgatg ctaacaatat ttacctagca accatgtatt cagaaacgcg taatatgacc
     5341 ccgtttggcg acagcgatta tgcagttgcg aacaaaaccc agaactttga agttactagt
     5401 accagtttga tttttgtctg cgtccggcag tctctttcct gatgtctaaa ggccgtgacc
     5461 ttcacgctgc tggtggtgca gacaacccgg caggtgttga tgataaagat ctggttaaat
     5521 acgccgatgt tggcgcgact tactatttca ataaaaacat gtccacctat gttgactata
     5581 aaatcaacct gttggatgaa gatgacagtt tctacgctgc caacggcatc tctaccgatg
     5641 atattgtcgc tttaggtctg gtttatcagt tctaaatcct cctgcccgct gttatggcgg
     5701 gctttttctg cttattcttc ctcctttgat ctaaattaaa aatgtgaact ccgtcattac
     5761 acaaaaagtg tcatctggcg ttacacttta tgcggatact aaaacaggag gttttatgaa
     5821 cagaacgatt cttgtcccta tcgatatttc agattcagaa ttaactcaac gcgtgattag
     5881 ccacgttgag gcagaggcaa agattgatga tgcagaggtt catttcctga cggtaatacc
     5941 ttcactgccc tactatgcct ctctgggttt agcgtattcc gcagaattac cggcaatgga
     6001 tgacctgaaa gcggaagcca aatcgcaact ggaagagatc attaaaaaat ttaaactgcc
     6061 aaccgacaga gtgcatgtcc atgttgagga aggctcgccc aaagaccgca ttctggaatt
     6121 ggcgaagaag atccccgctc atatgatcat cattgcttcc catcgaccgg atatcaccac
     6181 ttatctgctc ggttccaacg ccgcagctgt agtgcgtcac gcagagtgct ccgtgctggt
     6241 tgtgcgctga cactaacgcc cgcacattgc tgcgggcttt ttgattcatt tcgcaaatgt
     6301 gctgacattt tcccctctaa tccgtaccat acacgccaca gtttttatat cagatttctt
     6361 atgctgagcg ttccggcatg acgcatactc ttctgatgcc atataacgaa ttgagtcgct
     6421 tttaaatgtc gcaaaatcaa gaaattagta agaaagaaca atacaacctg aacaaattac
     6481 aaaaacgtct gcgtcgtaac gtgggcgaag ccattgctga cttcaatatg attgaagaag
     6541 gcgatcgcat catggtttgc ctctccgggg gtaaagacag ctataccatg ctggagattc
     6601 tgcgcaattt gcagcaaagc gcgccaatca atttttcgct ggtggctgtt aacctcgatc
     6661 aaaagcaacc gggcttcccg gaacacgttc tgcccgagta tcttgaaaag ctgggcgttg
     6721 agtacaagat tgttgaagag aacacttacg gtatcgtgaa agagaagatt ccagagggca
     6781 aaaccacttg ctcactgtgt tctcggctac gtcgcggtat cctttatcgc accgcaacgg
     6841 aactggggac gacgaagatc gcgttgggtc accatcgtga cgatatcctg caaacgttgt
     6901 tcttaaatat gttctacggc ggcaagatga aaggtatgcc tccgaaactg atgagcgatg
     6961 atggcaaaca tatcgttatt cgtccactgg cctactgccg cgagaaagat attcagcgat
     7021 ttgccgatgc aaaagcgttc ccgattattc cgtgcaacct gtgcggttca cagcctaacc
     7081 tgcaacgtca ggtgattgct gacatgttgc gtgactggga taaacgttat ccagggcgta
     7141 tcgagacgat gttcagcgcg atgcagaatg tggtgccgtc gcatctgtgc gataccaacc
     7201 tgttcgattt caaaggcatt acccacggtt ctgaagtggt taacgggggt gatctggcgt
     7261 ttgatcgcga agagatccca ctacaaccgg cgggctggca gccagaagaa gatgaaaatc
     7321 agttggatga gttacggctg aatgtggttg aagtgaaata accaggatag cgcccgatgc
     7381 gcaagcgtat cgggctactc ttatggaggc cggataagac gcggccagcg tcgcatctgg
     7441 caatcccgaa taagatgttt actcttgcac ccggcaattc aacatttcat tattttaata
     7501 accgcacccg gcacgttttt cctttaatct tcccgccctg taactgtttc catgctttat
     7561 gagcaacagc ctgacggacc gcgacataga catgcgccgg atgcacggcg attttgccaa
     7621 tatctgcgcc atcaagcccg atatctcctg tcagtgcacc taatacatca cccgggcgca
     7681 ttttggcttt tttcccgcca tcgatacaca acgttgccat ttctgcttcc agcgtcacaa
     7741 tggaactatt agctggcggc gtttgccagt taagttttat ctgcaacatg tcagaaatga
     7801 tattggcccg ctgtgcttct tccggagcac agaaactgat cgccagaccg ctatttcctg
     7861 cacgagctgt acgaccgatg cgatgtacat gaacttcagg gtcccacgcc agctcaaagt
     7921 tcaccaccag ctcaagcgat ttaatatcca gaccacgcgc agcaacatca gtcgcaacca
     7981 gtacacgggc gctaccgtta gcaaaacgta ccagggtctg atcgcgttgc tccagatcgc
     8041 cgtgtaacga caatgcactt tgctctactt cattcagcgc gtcgcagaca gcctggcaat
     8101 cttttttggt attgcaaaac accacacaag aggatggctg atgcaagctt aataaccgtt
     8161 gcaacagagg aattttgcct ttactggatg tctcataaaa ttgttgttca atgggtggca
     8221 aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg ttgcactcgt ccgctgattg
     8281 cggcgatggc ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa
     8341 aacggatgac atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat
     8401 ccatcaccaa agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca
     8461 aacgacccgg cgttgccacg ataatatgcg gcgcatgttg cagcgaatca cgctgcatac
     8521 cgaacggttg accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc
     8581 gacgcaattc acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct
     8641 gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt
     8701 tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg
     8761 cggcggcctg caccggcgtc atggttaaat aacccaactc attaaggttc gtgagttggg
     8821 cgggaggcaa aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac
     8881 gcaaagttag caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg
     8941 ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg
     9001 aacaggatcg ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat
     9061 atttccctcc ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa
     9121 aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa
     9181 aaaaggaagt aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca
     9241 agggagatat tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt
     9301 ttttgtatac agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc
     9361 ataaagcaac accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt
     9421 gccacccgcc gccagggatc ccaccaaggt tgacgccaaa taacccggtc agaaaggtac
     9481 tgggtaaaaa gaccattgcc atcaacgaca ttgtataggt acgacgagct aaattttcct
     9541 gcatcacctg agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt
     9601 cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc
     9661 tcatccacgg caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat
     9721 agcgacgcat cacaattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt
     9781 gctgatcaag gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt
     9841 cactggaatg atcggtcaac gcatcgcaca catccaccag ccatcccccg caatcggtcg
     9901 gacccgtgcc ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc
     9961 gggtcgaaac aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc
    10021 gttcatcggt gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgaaacggc
    10081 tgactcgggg ccgtgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg
    10141 gtgttgtcgc cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg
    10201 gatgcgcttc atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca
    10261 gcatccaggc aaatactgca tccgggacat taacgtccga tcccttaatc gcttccactg
    10321 tgcctccatc atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc
    10381 tcatcacccc attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc
    10441 aaaatgcttt tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa
    10501 ttatctgatc atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat
    10561 aacgggatct caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc
    10621 tggattgttt cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg
    10681 atgcaagacc tcacgtttag tcagctacga accggaccct attccgtctc ctcacaaaaa
    10741 gaactgccga aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact
    10801 gttcagcgta aagaagagga aacagcatta agctgtcggc ttgttttgcg agagctgaca
    10861 gaaacagaac cggtgattat tttcgaaggt atcgaagcgc cggcaacgct gggtttaaaa
    10921 gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg
    10981 actaactctg caccaatgtt gttgattgac ccgtcacgag atggacaaat cgtcgatgct
    11041 aacctcgccg cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc
    11101 taggaaataa atatgcttgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta
    11161 cccggcggtc ataacccttt gaattttgtt cataaactgg cggatggatc tactcgtcat
    11221 gtgcagacct atgccggacc gattgaaatt tatggcgaca agctcatgtt atgtattgtg
    11281 catgatatta ctgagcaaaa acggctggag gagcagctgg aacatgctgc tcaccatgac
    11341 gcgatgaccg gattactgaa tcggcgacag ttttatcaca ttacggaacc aggccaaatg
    11401 cagcatctcg ccatcgctca ggattacagc ttgttgctca tcgacaccga tcgttttaaa
    11461 cacattaacg atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc
    11521 cccctcgaaa gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt
    11581 gtcttattgc taccaagaac cccactggat accgcgcttt cgctggctga aactatccgc
    11641 gtaagcgtgg caaaagtgag tatttcgggc ttaccacgct ttaccgtcag cattggtgtg
    11701 gcgcatcacg aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg
    11761 tatcgggcga aaaatgatgg acgcaaccgc gtgctggcgg cataagccgc ggatgcgtct
    11821 cgagatcaac gactgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt
    11881 ttacgtagtg cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc ggtgcaatat
    11941 gcctgaacat catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca
    12001 tgtgatttat cttcccgccc tttaccatga ataatcagca cattacgcaa accatccgcc
    12061 agggcttgtt gaatgaaact gaacaccatt ttgcggcact cttccaccgg ctggcgcaaa
    12121 agattcaggc tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgc
    12181 tacaaccctt cctgccgaaa ctccagcggc tgacttagcg ggatgatgtc gagaaatccg
    12241 gtagtgagga aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc
    12301 gttggatgcc agtggacatc tgtagcacgt ttcagcggct ggacatcttc catggcgtca
    12361 agaaacagcg atttgtcgtc aaggttcatg taacctccta tgcaccttca cacgtcgtga
    12421 ttcagacggg ttaattgttc atacttactg catcgttccc ttcacagatt gaaacaatac
    12481 tctacttcat acctgtgagt atctgcgcag gcggggcatt ttatactagc ctgttagcag
    12541 tatcgaacag tttagcccga gtttaattca gcacaggctc cgatcaggat gatttattca
    12601 tagcaggaac caacaaaaat gcgataaata acagaccact gattagcaga aaggtaccga
    12661 aaatgccata gtttgcgcct ccctcagcat caattgactc aataatttgg ttgaagccag
    12721 tatttccgat gtctggtcac ttctactgcg attaaaaaat aaagcacttc tggaaatgaa
    12781 gtcattcacc gtatggggaa cagaaaccat tttttcaccc attctcgaaa aatgaaaatt
    12841 agttcgatta ttgagaatag agccacacag ggcaatacca atcgcgcccc caagattacg
    12901 tgttaaatta aatacaccag aggctaactt cagccgttct tttggaattc cccccagcgt
    12961 taatgttacg atgggggcca tcgcaaactg ctgcgaaatc cctctaattg cctgtggaaa
    13021 cagtaattcc tgccatcccc attcgtgggt tataggggta aaaagataca ttgaaaacac
    13081 aaatccgccc agccccgcca tcaacagcca tcgcagattt atttttttac ttaaccagaa
    13141 gtaaaaggga acagaaaata actggaaaat acctgttgtg caaacggcaa aacctatctc
    13201 ttcggcattt aatccgcgaa tctgccccaa aaacacaggt attaaatata cagtagagaa
    13261 tatcccaacc cctccagaaa aagaaaaaaa acatcccaga gtgaaatttt tatccttaaa
    13321 agcatgaaga tccattatcg gattggatat tgtaagtgta cgcgcagcaa ataaaataaa
    13381 actcaccaat gccagaactg atgtcagcaa aatagtatta tcatccagcc atccccagcg
    13441 cgctccttct tcaagggtat attccagaca tcccagagtc atggccaaca ggataatact
    13501 cggataatca gcaactttca gtaaagagag atcaggttta tcaaaattaa ccagaaaagg
    13561 aatactcaaa actaaatata tgccaggtaa tatattgata taaaatagcc atcgccagtc
    13621 gaggttatca gtgagccagc cccccagagt tggccctagc gtcggtgaca gtgaggccag
    13681 cgcactgact accgcagcag ccaaccccaa ctctttaccc tggtaataaa taaacgccat
    13741 cgtgaaaact aaagggatca tggatgcgcc agcagctccc tgtaaagcac gaaacaaaat
    13801 catgatctga atattccatg caagtccaca cgcgatactc attaaagtaa atattcccgc
    13861 ggatagagta aaaagccaac gagtggaaaa tacccgtgac agccacccag agagcggaat
    13921 aacaataatc tcagcaatta aatagctggt ctgaagccag gccatttcgt ctttacctgc
    13981 ggacagacca ccacctattt catttaggga tgacgataca atttgaatat caagctgagc
    14041 aataaataat cccatacaca tactggcgaa agcaatatgt ttacgcatag acatcatctc
    14101 tcatcaacac tgacggtgac ggacagtccc gggcgaagaa gttgctttaa ttcctttgcg
    14161 tcgtcaaatg caatccgtac cgggacacgc tgaacaattt tggtgaaatt gccagtggca
    14221 ttttctatcg gaatcaagct gaaactggct cctgtcgctg gggaaaggct ttcaatgtga
    14281 ccgtgaaaca cttccccttt gagaatatca gcacggatct cagccttcat tcctggcttc
    14341 attcccgcta tttggttttc tttataattt gcatcaaccc acaactcact gactggtact
    14401 agtgaaacta acgatgtgcc gccttccacc caactgcctg tatgggcgct gcggtttgcg
    14461 acaatgccat caatcggcgc a
//
LOCUS       DAZPAB010000094        14402 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZPAB010000094 DAZPAB010000000
VERSION     DAZPAB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.094"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            219..326
                     /locus_tag="ACHUPG_002494"
     CDS             219..326
                     /locus_tag="ACHUPG_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0862075.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            702..809
                     /locus_tag="ACHUPG_002495"
     CDS             702..809
                     /locus_tag="ACHUPG_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0862076.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(896..2575)
                     /gene="bcsG"
                     /locus_tag="ACHUPG_002496"
     CDS             complement(896..2575)
                     /gene="bcsG"
                     /locus_tag="ACHUPG_002496"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR0862077.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2572..2763)
                     /gene="bcsF"
                     /locus_tag="ACHUPG_002497"
     CDS             complement(2572..2763)
                     /gene="bcsF"
                     /locus_tag="ACHUPG_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR0862078.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2760..4331)
                     /gene="bcsE"
                     /locus_tag="ACHUPG_002498"
     CDS             complement(2760..4331)
                     /gene="bcsE"
                     /locus_tag="ACHUPG_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR0862079.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            4604..4792
                     /gene="bcsR"
                     /locus_tag="ACHUPG_002499"
     CDS             4604..4792
                     /gene="bcsR"
                     /locus_tag="ACHUPG_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR0862080.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4804..5556
                     /gene="bcsQ"
                     /locus_tag="ACHUPG_002500"
     CDS             4804..5556
                     /gene="bcsQ"
                     /locus_tag="ACHUPG_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR0862081.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            5553..8170
                     /gene="bcsA"
                     /locus_tag="ACHUPG_002501"
                     /pseudo
     CDS             5553..8170
                     /gene="bcsA"
                     /locus_tag="ACHUPG_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            8181..10520
                     /gene="bcsB"
                     /locus_tag="ACHUPG_002502"
     CDS             8181..10520
                     /gene="bcsB"
                     /locus_tag="ACHUPG_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR0862082.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGTPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            10527..11633
                     /gene="bcsZ"
                     /locus_tag="ACHUPG_002503"
     CDS             10527..11633
                     /gene="bcsZ"
                     /locus_tag="ACHUPG_002503"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR0862083.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            11828..12217
                     /locus_tag="ACHUPG_002504"
     CDS             11828..12217
                     /locus_tag="ACHUPG_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001391818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase subunit BcsC-related outer
                     membrane protein"
                     /protein_id="HFR0862084.1"
                     /translation="MISNAACKTTVWHSRQRYWKTRANGRRRQHFSGNDWRWAPAAYG
                     LLTDWQEEAARQSNDGGSSQGFGYTARALLERRVTSNWFVGTAIDIQQVKDYAPSHFL
                     LYVRYSAAGWQGDMDLPPQPLIPYADW"
     gene            12299..14287
                     /gene="hmsP"
                     /locus_tag="ACHUPG_002505"
     CDS             12299..14287
                     /gene="hmsP"
                     /locus_tag="ACHUPG_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR0862085.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
BASE COUNT         3396 a         3667 c         3948 g         3391 t
ORIGIN      
        1 ccggaacaga aaaacagtcg tagaatcagc gtcaagccgg aagggttccg gtgaggcgca
       61 atgttgcggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa cccccgcacg
      121 ttgcaggtat gcacctgaca acatcacggg ggccattctt gaacctgaac aactcaagaa
      181 tagccgcgaa acgttgtcat tacaacacag gcggctatat gacgctcgca gaactgggca
      241 tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc agtatgatcg
      301 tgaactggct gaacaagcgg aagtaacgtg tcatgcgggc gtcaggctgc cgtaatggca
      361 atttgcgccc ggaccaggcc gcaggggtga aactctgcgg cctttttcgt aagaaaaatg
      421 caacgattgc acgcggataa tattctgcgg aatgcgcagc acagagtgaa gacgtgacgg
      481 attccggaac agaaaaacag tcgtagaatc agcggcaagc cggaaaggtt ccggtgaggc
      541 gcaatgttgc gggggcttta tccctggtgg cattggttgc tggagagaga aaacccccgc
      601 acgttgcagg tatgcacctg acaacatcac gggggctaat cttgaaccca aacaactcaa
      661 gaatagccgc gaaacgttgg cattacaaca caggcggcta tatgacgctc gcagaactgg
      721 gcatggcctt ctggcatgat ttagcggctc cggtcattgc tggcattctt gccagtatga
      781 tcgtgaactg gctgaataag cggaagtaac gtgtcatgcg ggcgtcaggc tgccgtaatg
      841 gcaatttgcg cccggatcag gccgcagagg ttaaactctg cggccttttt cgtgcttact
      901 gcgggtaagg cacccagtcg ccgccgttca ggcgaacgta cggtttatct tggtattgaa
      961 taactactgc atttgagttc tcggagaccg gtgctgtttg tggcaaccca ctggtgagtt
     1021 ttttccagtc aacattgtct tcggtgaaaa tcttgccatc aagaacgcga accaccagat
     1081 cggagatagc caggaagctg ctcggttggt caatgacaat cggtgccccc tgatgtggtg
     1141 ccttcatgcc gaagaatttc accccaacgg ggacgtctgt gatagacggg ctagggatat
     1201 cacgtaggcc agatacctgc attctgtcgc ccttcagcgc gccgccgtgt tccggcacca
     1261 cgaccaccat cactttacga cccgatttct ccagttcagt aaagaaggcg tccagttcat
     1321 caaagaattt ctgcgcccgc gctttgtaat ccgctgtttt gctgaccccc ggataatggt
     1381 tgccgtcatg cagtggaagc gtgttgtaga acgtggcact acggctgttt ttatcttttt
     1441 cggtaacgtc cagccagcgg ttaagcacgg cggtatcgtc ataaaccggc gaaccatcaa
     1501 agcccagcaa aataaccggc agatttgttt gatccatcaa ttcagtctgc atgccgccat
     1561 tttcgcgaac ttctttcaaa aaaccgccga actggccgtt atgtcccatc atcaggtgct
     1621 gggtaaagcc cagtttcgaa aggttatcaa acagatagca gtcgttattt gccggttgat
     1681 acagattagt gtgcgaagtc tgcccgcagc tggcgcgcag taaacggatc gccgccgggc
     1741 cactgtagga ggtggcggag ttaaagttct tgaaatcaat atcgaaatgc gaccacagtg
     1801 gatgcgacat caacccggcg gcttctatat ccgaccagga aagcgaacag atgttaatca
     1861 ccagtagttc aaatggctga gcatcagcgg gcagcgaaga cgggaaggtc gatttacgtt
     1921 tcgcctccgc gttatagaaa ttattcagcc aggcgttaag gttcgccgtt gttggcggtg
     1981 cagtttgtgc gggcatatca cccactaccg gtgcgccacc cgtcgccgca acggttgccg
     2041 ctgcgttacc acccgtcgtt gttacagtgg tcgtcggttg tccggctggc cacaaggaga
     2101 aacttggtcc cgccagggta agtacgttca gccatagcag tatggcaacc acaaaaacgg
     2161 taatgcgaat ccattgtgac aggaataacc aggccactaa taaaacaaaa atggccccaa
     2221 tcatctgcca gttaataaag cgtgtgacaa ggtcgattaa ataatcggta ctgaaccccg
     2281 ccacctgcga accctggctc attatgcttt ccgggccagg caaccagatg tcatgccaga
     2341 acaaagcaaa gccgatcggc agggcaatcc agtggcgcaa gcgatgcagg ctgtaacgcg
     2401 gaatgggcat cagcagaaac gcggcaaaca ccaaattgag gagcgggtgg aagttaagat
     2461 atcccgccca caacaggcca aacttaacca gaaaatagaa gttccagccg gaaaggccgc
     2521 gccagtattg ccagagggaa gaaggcatgg cggtattttg cgtaaattga gtcatttttt
     2581 ggttgccctg gctttttccg tgcggcgtaa cgtcccggcc ggtttaacat aacgaggttt
     2641 agcaaagaac aaacgtaagg tgtcgcgaat gcgtcgcaaa gagtgccgcg ccagatagcc
     2701 cagcgggaaa aatatcagtg cgcaaacgat aataatttca atgatatcgc tgatggtcat
     2761 catgatgagc gctccacagc atcatctaac agtcgcatgg gttctggtat tcgtcgccag
     2821 tggcgaccat cgtgctcggc attgatgacc ggtttagaac tttgcgttaa aggcagcggc
     2881 attccccatt gttctggggc aagcaggcgc atctgcacca gctcggcact gatttgatca
     2941 tcttcaaacc agaccatacg gtttgagaaa atgtcgccag taggcaatgg gaaaatatga
     3001 ttcaacgcgg tatccagatc gttaatccga cagaatgaga gaaacagcac cagccgatta
     3061 ccgccaatgg tcatgatatc gccggtacgg ttagggcgac atagcgtcag ggcttgctca
     3121 acgcggatac ccggtaccgg acgtagggca accagaacgc ctttaccgtg ggcaggcaag
     3181 agagggttat tcatcatgtt gttgacggca ttacagaaca catcccactt ctggaaacca
     3241 cgcagtttga gcggctgggt cattgacagc aaggtagtga tatcttccgg tacatagcga
     3301 ctaaacttct gcccttgcac gctttcgatc atcgtcagac aacgggagag tggcgcattc
     3361 cacggaataa ccatatttgc accgcaggcc aataacaaac gttcatcggt ggcgcgcagg
     3421 ctagcggtat tttcccgcac gaggattttc atcgcactac cgcgctggcg acgcagggta
     3481 tgaatgctgc gggccagtgg ctcgatttgc gcattttgct gtaaagaaaa gaccaccgtc
     3541 gccgcctgag cggtacgggc ttcattgaac aggacttcgt tattgttgaa cagttgccag
     3601 tgttccgata gcggcggcgc accttccagt acagcaacat tactcagaat gcgtttttcg
     3661 tcgctgcgtg gttggatctc cgcctcttcg ctttgaacta atgtccagat accattttgt
     3721 tgctgaacgc taagctgctg acgggcgctg accccttttt cgttgcacca gaaggcaata
     3781 tccagcaaat gttggtcacc ctgaaaacgc aaactggcaa gaccaaaaag tgaacggtat
     3841 tcctcaagca acaatgaaaa ttgtttatcg ttattatttc cgggattaat taccaaaagc
     3901 gaacaatggt ttaacctgct ccatttattc attttatcca accatgagcg aagccgctcg
     3961 gcaggaatat tttgccatgc gttatttgcg caaacaagaa taaaaaggta attatgggga
     4021 tcaatagaac actgcaaatc acgggccaaa tagtatagac ctttttcatg atttagcatt
     4081 gaaaataatt ttattttttc cggcccttga gaatcatcta attgaaagat tttcgccgga
     4141 tcgctgtcca tgctaatgac cgcgacgtgt gcggtttcag cctgggatgc aattgtttga
     4201 ttcgccagac tgatagcatc ttcatggcga tcgacgttaa accaccagac gccgcctgct
     4261 ggcatatgtc gcagctcatc ccataatgat gagataccga tagagaatac agggtccaca
     4321 atgtccctca tgtcaccatt ttgtctgatt atcagtttac tagcgaaagc taagaaataa
     4381 acctaccatt gaaattaaaa cttatcagat ttagcatgtg aatcaattta gaaggtcaca
     4441 agacgtgtga ttttagtctt ttcgttttta actttttctg ttgcgtaacg gaaagtcaaa
     4501 aagtgagcaa attcccgtct cgccgtaaac aataaaccgg cgaaatgctc acggttagaa
     4561 ttgtctcatg aatggtacgg ttatttcata gggatcaagc aaaatgaata acaatgaacc
     4621 agatactctg cctgatcccg cgataggcta tatcttccag aatgatattg tggcgttaaa
     4681 gcaggcattt tcactgcctg atattgatta tgccgatatt tcccaacgcg aacagttggc
     4741 cgcggcatta aaacgctggc cgttgctggc agagtttgcg caacaaaagt aggggattgg
     4801 tgaatggccg tactgggatt gcagggggtg cggggaggcg tggggacaac aaccatcacc
     4861 gccgcattag cctggtcatt acaaatgttg ggagaaaatg tcctggtggt cgatgcctgc
     4921 ccggacaact tgttgcgcct gtcatttaat gttgatttta cccaccgtca gggctgggcc
     4981 agagcgatgc tggatggcca ggactggcgt gacgctgggt tgcgctacac ctcgcagctc
     5041 gatttgctgc cttttggtca gttatccatt gaagaacaag aaaatccaca gcactggcaa
     5101 acccggctga gcgatatttg ctccggctta cagcaactaa aagccagcgg gcgttaccag
     5161 tggattttaa tcgacttacc gcgtgatgcc tcgcagataa cccaccagct gctgagtttg
     5221 tgcgagcact cgctggcaat cgtcaatgtg gatgccaact gccatatccg actgcatcag
     5281 caagcgctgc cggatggcgc acatattttg attaatgact tccgtattgg cagtcaggtt
     5341 caggacgata tttaccagct ttggttgcaa agccagcgcc gattactgcc gatgctcatt
     5401 catcgtgatg aagcgatggc tgaatgcctg gcggctaagc aaccagtagg tgaatatcgc
     5461 agtgatgcgc tggcggctga agagatactg acgctggcga actggtgcct gttgaactac
     5521 tccgggctga aaacgccagt cgggagtgca tcatgagtat cctgacccgg tggttgctta
     5581 tcccgccggt caacgcgcgg cttatcgggc gttatcgcga ttatcgtcgt cacggtgcgt
     5641 cggctttcag cgcgacgctc ggctgtttct ggatgatcct ggcctggatt tttattccgc
     5701 tggagcaccc gcgctggcag cgtattcgcg cagaacataa aaacctgtat acgcatatca
     5761 acgcctcgcg tccgcgtccg ctggacccgg tccgttatct cattcaaaca tgctggttat
     5821 tgatcggtgc atcgcgcaaa gaaacgccga aaccgcgcag cgggcatttt caggtctgca
     5881 aaatattcgt ggacgttacc atcaatggat gaacgagctg cctgagcgcg ttagccataa
     5941 aacacagcat ctggatgaaa aaaaagagct cggtcatttg agtgccgggg cgcggcggtt
     6001 gatcctcggt atcatcgtca ccttctcgct gattctggcg ttaatctgcg ttactcagcc
     6061 gtttaacccg ctggcgcagt ttatcttcct gatgctgctg tggggggtag cgctgatcgt
     6121 acggcggatg ccggggcgct tctcggcgct aatgttgatt gtgctgtcgc tgaccgtttc
     6181 ttgccgttat atctggtggc gttacacctc tacgctgaac tgggacgatc cggtcagcct
     6241 ggtgtgcggg cttattctgc tcttcgctga aacgtacgcg tggattgtgc tggtgctcgg
     6301 ctacttccag gtagtatggc cgctgaatcg tcagccggtg ccattgccga aggatatgtc
     6361 gctgtggccg tcagtggata tctttgtccc gacttacaac gaagatctca atgtggtgaa
     6421 aaataccatt tacgcctcgc tgggtatcga ctggccgaaa gacaaactga acatctggat
     6481 cctcgatgac ggcggcaggg aagagtttcg ccagtttgcg caaaacgtgg gggtgaaata
     6541 tatcgcccgc accactcatg aacatgcgaa agcgggcaac atcaacaatg cgctgaaata
     6601 tgccaaaggc gagttcgtgt cgattttcga ctgcgaccac gtaccaacgc gatcgttcct
     6661 gcaaatgacc atgggctggt tcctgaaaga gaaacagctg gcgatgatgc agactccaca
     6721 ccacttcttc tcgccggacc cgtttgaacg caacctggga cgttttcgta aaacgccgaa
     6781 cgaaggcacg ctgttctatg gtctggtgca ggatggcaac gatatgtggg acgccacttt
     6841 cttctgcggt tcctgtgcgg tgattcgtcg taagccgctg gatgaaattg gcggcattgc
     6901 tgtcgaaacc gtgactgaag atgcgcatac ttctctgcgt ttgcaccgtc gtggctatac
     6961 ctccgcgtat atgcgtattc cgcaggcggc ggggctggcg accgaaagtc tgtcggcgca
     7021 tatcggtcag cgtattcgct gggcgcgcgg gatggtataa atcttccgtc tcgataaccc
     7081 gctcaccggt aaaggactga agtttgctca gcggctgtgc tacgtcaacg ccatgttcca
     7141 cttcttgtcg ggcattccac ggctgatctt cctgactgcg ccgctggcgt tcctgctgct
     7201 tcatgcctac atcatctatg cgccagcgtt gatgatcgcc ctattcgtgc tgccgcatat
     7261 gatccatgcc agcctgacca actcgaagat ccagggcaaa tatcgccact ctttctggag
     7321 tgaaatctac gaaacggtgc tggcgtggta tatcgcacca ccgacgctgg tggcgctgat
     7381 taacccgcac aaaggcaaat ttaacgtcac cgccaaaggt ggactggtgg aagaagagta
     7441 cgtcgactgg gtgatctcgc ggccctacat cttccttgtt ctactcaacc tggtgggcgt
     7501 tgcggtaggc atctggcgct acttctatgg cccgccaacc gagatgctca ccgtggtcgt
     7561 cagtatggtg tgggtgttct acaacctgat tgttcttggc ggcgcagttg cggtatcggt
     7621 agaaagcaaa caggtacgcc gatcgaaccg cgtggagatg acgatgcccg cggcaattgc
     7681 ccgcgaagat ggtcacctct tctcgtgtac cgttcaggat ttctccgacg gtggtttggg
     7741 gatcaagatc aacggtcagg cgcagattct ggaagggcag aaagtgaatc tgttgcttaa
     7801 acgcggtcag caggaatacg tcttcccgac ccaggtggcg cgcgtgatgg gtaatgaagt
     7861 tgggctgaaa ttaatgccgc tcaccaccca gcaacatatc gattttgtgc agtgtacgtt
     7921 tgcccgtgcg gatacatggg cgctctggca ggacagctac ccggaagata agccgctgga
     7981 aagtctgctg gatattctga agctcggctt ccgtggctac cgccatctgg cggagtttgc
     8041 gccttcttcg gtgaagggca tattccgtgt gctgacttct ctggtttcct gggttgtatc
     8101 gtttattccg cgccgcccgg agcggagcga aacggcacaa ccatcggatc aggctttggc
     8161 tcaacaatga tgataacgcg atgaaaagaa aactattctg gatttgtgca gtggctatgg
     8221 ggatgagtgc gttcccctct ttcatgacgc aggcgacgcc agcaacgcaa ccactgatca
     8281 atgctgagcc agctgtagcc gcccagacgg aacaaaatcc gcaggtgggg caagtgatgt
     8341 caggcgagca gggagctgat gcccccatcg tggcgcagaa cggtccttcg cgtgatgtga
     8401 agctgacctt tgcgcaaatc gcgccgccgc cgggcagcat ggtgctacgt ggtattaacc
     8461 cgaacggcag cattgagttt ggtatgcgca gcgatgaagt ggtgacgaag gcgatgctca
     8521 acttcgaata cacgccatcg ccatcgttac tgcctgtcca gtcgcagtta aaggtttatc
     8581 tcaatgatga gctgatgggc gtgctgccag tgaccaaaga acagttgggt aaaaaaacgc
     8641 tggcgcaaat gcccattaat ccactgttta ttaccgactt caaccgtgtg cggctggagt
     8701 ttgtcggcca ttatcaggac gtgtgcgaaa acccggccag caccacgctt tggctggatg
     8761 ttgggcggag cagtggactg gatctgacct atcagaccct gaatgtgaag aatgacctgt
     8821 cacacttccc ggtgccattc tttgacccgc gcgataaccg tactaacacc ttgccgatgg
     8881 tctttgcggg tacgccggat gttgggctgc aacaagcatc tgccattgtc gcctcgtggt
     8941 ttggttcgcg ttctggctgg cgtgggcaga acttcccggt gctctataac caattgccgg
     9001 atcgcaatgc cattgtcttt gcaaccaacg acaaacggcc ggacttcctg cgcgatcatc
     9061 cggcggtaaa agccccggtg attgagatga ttaaccatcc gcagaatcct tacgtcaaac
     9121 tgctggtggt gtttggtcgt gacgacaaag acctgttgca ggcagcgaaa ggtatcgctc
     9181 agggtaacat tctgttccgt ggtgaaagcg tggtagtgaa tgaagtgaaa ccgctgctac
     9241 cgcgtaagcc gtacgatgcg ccgaactggg tacgtaccga tcgtccggtc acatttggcg
     9301 aactgaaaac ctatgaagaa cagttacaat ccagcggcct tgagccagca gcgattaacg
     9361 tttcgctaaa cctgccgccg gatctctacc tgatgcgcag taccggcatt gatatggata
     9421 ttaattaccg ctacaccatg ccgccggtga aagacagttc gcggatggat atcagcctga
     9481 ataaccagtt cctgcaatcc ttcaacctga gcagcaaaca ggaggcgaac cgtctgctgc
     9541 tgcgtattcc ggtattacaa ggtttgctgg atggcaaaac agatgtctct attccggcgc
     9601 tgaaactggg cgcgaccaac cagctgcgct tcgactttga gtatatgaac ccgatgccag
     9661 gcggttcggt agataactgt attaccttcc agccggtgca gaatcatgtg gtgattggtg
     9721 acgattccac catcgacttc tcgaagtatt accacttcat cccgatgccg gatctacgcg
     9781 cctttgctaa cgcgggcttc ccattcagcc ggatggcgga tctgtcgcaa accatcaccg
     9841 tgatgccgaa agcgcctaac gaagcacaga tggaaacgtt gctgaatact gttggtttta
     9901 tcggcgcaca gacgggcttc ccggcgatta atctgacggt gaccgatgat ggcagcacca
     9961 ttcagggcaa agatgccgac atcatgatca tcggtggtat cccggacaaa ctgaaagacg
    10021 ataagcagat cgacctattg gtgcaggcga ccgaaagctg ggtgaaaaca ccgatgcgcc
    10081 agaccccgtt ccccggcatt gtgccggacg agagcgatcg cgcggcagaa acccagtcaa
    10141 cgctgacctc ttccggtgcg atggcggcgg tgattggctt ccagtcgccg tataacgacc
    10201 agcgcagcgt gattgcgctg ttggcagata gcccacgcgg ttatgaaatg cttaacgatg
    10261 cggtgaacga tagcggcaaa cgcgccacca tgttcggttc ggtcgcggtg atccgcgagt
    10321 ccggtatcaa cagcctacgt gttggcgacg tttattacgt aggtcatctg ccgtggttcg
    10381 agcgcttgtg gtatgcgctg gcaaaccatc cgattctgct ggcggtgctg gcgactatca
    10441 gtgtgatatt gctggcatgg gtactgtggc gtctgctgcg aattattagt cgtcgtcgtc
    10501 ttaacccgga taacgagtaa ttgaagatga atgtgttgcg tagtggaatc gtgacgatgc
    10561 tgttgttggc tgcctttagt gttcaggcag cctgtacctg gcctgcctgg gagcagttta
    10621 aaaaggatta catcagtcag gaagggcgcg tcatcgaccc cagcgacgcg cgcaaaatca
    10681 ccacctccga agggcaaagt tacggtatgt tctttgccct ggcggctaac gaccgtgcag
    10741 ctttcgataa tattctcgac tggacgcaga acaatctcgc tcagggttct ttaaaagaac
    10801 gtttgcccgc ctggctgtgg ggcaagaaag agaacagtaa gtgggaagtg ctggacagca
    10861 attcggcctc cgatggtgat gtctggatgg cctggtcgtt gctggaggcg gggcgtttgt
    10921 ggaaagagca gcgttatacc gacatcggca gcgcgttgct aaaacgtatc gcgcgggagg
    10981 aagtggtgac ggtgcctggg ctgggttcca tgttgttacc gggcaaagtg ggttttgctg
    11041 aggataacag ctggcgtttt aaccccagct acatgccgcc gacgctggcg cagtatttca
    11101 cccgctttgg cgcgccgtgg accacgctgc gcgaaaccaa tcaacattta ttgctggaaa
    11161 ccgccccgaa aggcttttcg ccagactggg tgcgctatga gaaagacaaa ggctggcagc
    11221 taaaagccga aaaaacattg atcagcagct acgacgctat ccgcgtttac atgtgggtag
    11281 gcatgatgcc tgacagcgat ccgcaaaaag cgcggatgct caaccggttt aaaccgatgg
    11341 cgacattcac tgagaaaaac ggttatccgc cggaaaaagt ggatgtggct acggggaaag
    11401 cgcagggtaa aggaccggtc ggtttttctg ccgccatgct gcctttttta caaaaccgcg
    11461 atgcgcaggc agttcagcgc cagcgcgtgg ccgataactt tcccggcagc gatacctatt
    11521 acaactatgt gctgaccctg tttggacaag gctgggatca acaccgtttc cgcttctcga
    11581 caaaaggtga gttattacct gactggggcc aggaatgcgc aaattcacac taaacatatt
    11641 cacgctttcc ctcggtctgg ccgtcatgcc gatggtcgag gcagcaccaa ccatcagcag
    11701 accctgcgga tggacagcgg caacactaat gccgtgcgcg ggctggcgaa tatttaccgc
    11761 cagcagtcgc cagaaaaagc tgaagcgttt atcgcctcgc tctctgccag ccagcggcgc
    11821 agcattgatg atatcgaacg cagcctgcaa aacgaccgtc tggcacagca ggcagaggta
    11881 ctggaaaacc agggcaaatg ggcgcaggcg gcagcacttc agcggcaacg actggcgctg
    11941 ggccccggca gcgtatggat tacttaccga ctggcaggaa gaagctgcgc ggcaatccaa
    12001 cgatggcggc agcagtcagg gcttcggcta cacggcgcgg gcattacttg aacgacgtgt
    12061 tacttccaac tggtttgttg gcacggcaat tgatatccag caggtgaaag attacgcacc
    12121 cagccatttc ctgctctacg tacgttattc cgccgccgga tggcagggtg acatggattt
    12181 accgccgcag ccgctgatac cttacgccga ctggtaagtt ttcagatagc gcctctctta
    12241 atgccgctgc gatcgggtat actcgggcgg caatctggga tttccggggg gagacaattt
    12301 gcgcgtaagt cgctcgttaa caatcaagca gatggcaatg gtggcagccg ttgtcctggt
    12361 gttcgttttt attttttgca ccgttttgct gttccatctg gtccagcaga atcgctataa
    12421 cacggctacg caactggaaa gcattgctcg ctctgtccgc gaacccttat cttcagctat
    12481 tttgaaaggc gatattcccg aagcggaagc tattcttgcc agcattaaac cggcaggcgt
    12541 ggtcagccgt gccgatgtag tgctgcctaa ccagttccag gcgctgcgta aaagttttat
    12601 tccagagcgt ccggtgccgg taatggttac tcgcctgttt gagctaccgg ttcaaatctc
    12661 gctgggcgtc tactcgctcg aacgtccggc aaatccgcag ccaatagcct atctggtgct
    12721 acaggcggat tccttccgta tgtataagtt cgtgatgagc actctctcaa cgttagtgac
    12781 catttactta cttttgtcgc ttatcctgac cgtcgccatc agctggtgca ttaaccgcct
    12841 gattttgcat ccgttacgca atattgctcg cgaacttaac gccatcccag cccaggagct
    12901 tgttggtcac caactggcat taccgcgtct gcatcaggac gatgaaatcg gtatgttggt
    12961 gcgcagttac aacctcaacc agcaattgct gcagcgccat tatgaagaac agaacgaaaa
    13021 tgcgatgcgc ttcccggtgt cggatttgcc gaacaaagcc ttgctgatgg agatgctgga
    13081 gcaggttgtc gcgcgtaaac aaaccaccgc gctgatgatc atcacctgtg aaaccctgcg
    13141 tgatactgcg ggcgtgctga aagaggcgca acgagaaatt ctgctgctga cgctggtgga
    13201 aaaactcaaa tcggtactgt cgccacgtat gatcctcgcg cagattagcg gttatgactt
    13261 tgctgtcatt gccaacggtg tacaggaacc gtggcacgca atcactttgg gtcagcaagt
    13321 actcactatc atgagcgagc gcctgccgat tgaacgtatt caactccgtc cgcactgtag
    13381 cattggcgtg gcgatgttct acggcgatct caccgccgaa cagctttaca gtcgcgctat
    13441 ttctgcggca tttaccgctc gccataaagg caagaatcag attcagttct ttgatccgca
    13501 gcagatggaa gcagcccaga agcggttgac ggaagagagc gatatcctta atgcactgga
    13561 aaatcatcag tttgcaattt ggttacagcc acaggtcgag atgaccagcg gtaaactggt
    13621 cagtgcggaa gtgttactgc gtatccagca accggatggc agttgggacc tgccggatgg
    13681 cttaatcgat cgcattgagt gctgtgggct gatggttacc gtcggtcact gggtgctgga
    13741 agagtcctgt cgattgcttg cagcctggca agagcgcggc attatgctgc ccttgtcggt
    13801 aaacctctct gcgctgcaac tgatgcaccc gaatatggtg gcggatatgc tggaactgtt
    13861 aacccgctat cgcattcagc cgggaacact gattctggaa gtgacagaaa gccgacgtat
    13921 tgacgaccct catgctgcgg tggcaatcct ccgtccgctg cgcaatgccg gagttcgggt
    13981 ggcgctggat gatttcggca tgggctacgc agggctgcgt cagctgcagc atatgaaatc
    14041 gttgccaatc gacgtactga aaatcgacaa aatgtttgtt gaaggcttgc cggaagatag
    14101 cagcatgatt gctgcaatta tcatgctggc gcagagcctg aacttacaaa tgattgccga
    14161 aggcgtggag actgaagcac aacgagactg gctggcaaaa gcgggcgttg gtattgccca
    14221 gggcttcctt tttgctcgcc cactccctat tgaaatcttc gaagagagtt acctggaaga
    14281 aaagtagcta ccccaaactg attacaaaac tttaaaaagt gctggtttgt gcgagccagc
    14341 tcaaactttt taaccttttt gtttcaatta tgatccaggt acatttctgt gatgttgtct
    14401 gg
//
LOCUS       DAZPAB010000095        14342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZPAB010000095 DAZPAB010000000
VERSION     DAZPAB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.095"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            26..592
                     /locus_tag="ACHUPG_002506"
     CDS             26..592
                     /locus_tag="ACHUPG_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0862086.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            643..3102
                     /locus_tag="ACHUPG_002507"
     CDS             643..3102
                     /locus_tag="ACHUPG_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0862087.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKIPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DGNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3117..3848
                     /locus_tag="ACHUPG_002508"
     CDS             3117..3848
                     /locus_tag="ACHUPG_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0862088.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3845..4921
                     /locus_tag="ACHUPG_002509"
                     /pseudo
     CDS             3845..4921
                     /locus_tag="ACHUPG_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            5073..6119
                     /locus_tag="ACHUPG_002510"
     CDS             5073..6119
                     /locus_tag="ACHUPG_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR0862089.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6116..6907)
                     /gene="nei"
                     /locus_tag="ACHUPG_002511"
     CDS             complement(6116..6907)
                     /gene="nei"
                     /locus_tag="ACHUPG_002511"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR0862090.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6943..7677)
                     /gene="pxpA"
                     /locus_tag="ACHUPG_002512"
     CDS             complement(6943..7677)
                     /gene="pxpA"
                     /locus_tag="ACHUPG_002512"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR0862091.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(7667..8599)
                     /gene="pxpC"
                     /locus_tag="ACHUPG_002513"
                     /pseudo
     CDS             complement(7667..8599)
                     /gene="pxpC"
                     /locus_tag="ACHUPG_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(8593..9249)
                     /gene="pxpB"
                     /locus_tag="ACHUPG_002514"
     CDS             complement(8593..9249)
                     /gene="pxpB"
                     /locus_tag="ACHUPG_002514"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR0862092.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(9272..10015)
                     /gene="ybgI"
                     /locus_tag="ACHUPG_002515"
     CDS             complement(9272..10015)
                     /gene="ybgI"
                     /locus_tag="ACHUPG_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR0862093.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10286..11767
                     /gene="dtpD"
                     /locus_tag="ACHUPG_002516"
     CDS             10286..11767
                     /gene="dtpD"
                     /locus_tag="ACHUPG_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR0862094.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11917..13335)
                     /gene="phrB"
                     /locus_tag="ACHUPG_002517"
     CDS             complement(11917..13335)
                     /gene="phrB"
                     /locus_tag="ACHUPG_002517"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFR0862095.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(13332..13841)
                     /locus_tag="ACHUPG_002518"
     CDS             complement(13332..13841)
                     /locus_tag="ACHUPG_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR0862096.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            13970..14257
                     /locus_tag="ACHUPG_002519"
     CDS             13970..14257
                     /locus_tag="ACHUPG_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
                     /protein_id="HFR0862097.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
BASE COUNT         3482 a         3844 c         3507 g         3509 t
ORIGIN      
        1 ttcttttctg ttaaaggact cagttatgtt taaaggacaa aaaacattgg ccgcactggc
       61 cgtatctctg ctgttcacag cacctgttta tgcggctgat gaaggatccg gtgaaattca
      121 ctttaaaggt gaagttattg aagcaccgtg tgaaatacat caggatgata ttgataaaga
      181 ggttgaactc ggtcaggtga ccaccagcca cattaatcag tcacatcaca gcgatgccgt
      241 tgctgtcgac ctgctcttag tcaactgtga tctggaaaac tccagcaacg gttccggtgg
      301 caagatttct aaagttgcag tcacctttga tagctcagcg aaaaccaccg gcgcagatcc
      361 gattctcaac aacaccagca cgggtgaagc caccggcgtc ggcgtacgtt taatgaataa
      421 agatcaaagc aatatcgttt taggcaccgc tactccagat atcgacctgg ctccgacctc
      481 cagcgaacaa acgctgaatt tctttgcctg gatggaacaa attgatcagg ctacacctgt
      541 aacgccaggc gcagttacgg cgaatgcaac ctacgtgctg gattataaat aagtattact
      601 ttggcggcgc tatcgccgcc tttcataaaa tatttatcag gtatggacac cgtgaatatt
      661 tatcgactct cttttgtatc ctgcctggtc gtggcgatgc cttgcgcatt ggcggtcgaa
      721 ttcaatctga atgttctcga taaatcaatg cgcgaccgca ttgatatttc attattaaag
      781 gaaaaaggag tcattgctcc cggtgagtat tttgttagcg ttgcggtaaa taataaccaa
      841 atcagtaacg agcaaaagat taactggcac aaaaatgacg ataaaaccat tccgtgcatc
      901 aatgatttac tggtcgataa atttggctta aaacctgaag tccgtcagtc gttaccattg
      961 ataaatcagt gcgtcgattt tagctcccga cctgaaatgc tcttcaattt cgatcaagcc
     1021 aatcagcaac tgaatattag tattccgcaa gcctggctgg cgtggcactc agaaaactgg
     1081 accccaccct ccacatggaa agaaggtgtc gccggtgtcc tgatggatta caacttgttt
     1141 gccagcagct accgcccaca ggatggcagc agcagcacta atcttaacgc ctatggaaca
     1201 gccggaatca acacgggggc atggcgctta cgcagtgatt atcagttgaa tcatactgat
     1261 agcgatgata accatgaaca gtcaggcgaa atatcacgta cctatctttt tcgcccgtta
     1321 ccgcaattag gttcaaaatt aaccctcggc gaaacggatt ttagtcccaa tattttcgac
     1381 ggtttttctt ataccggcgc ggcactggca agtgacgatc gaatgttgcc atgggaacta
     1441 cgcggctacg ccccacaaat tagcggtatt gcacagacca atgccacggt gacgatcagt
     1501 caatcaggcc gcgtcattta ccagaaaaaa atcccaccag gcccatttat cattgacgac
     1561 cttaatcagt ctgttcaggg cacactggat gtcaaagtga cggaagaaga tggtcgggtg
     1621 aacaatttcc aggtttcagc agcatcgacg cccttcctga ctcgtcaggg acaggttcgc
     1681 tataaactgg ccgcgggtca gccacggccc tccatgtcac atcaaactga aaatgaaacc
     1741 ttttttagca atgaagtttc ctgggggatg ctgtcaaaca cctcgctgta cagcggcctg
     1801 ctgctttctg gtgatgacta ccattctgcc gcaatgggta ttgggcaaaa tatgctgtgg
     1861 cttggtgcgc tgtcgtttga tgtcacgtgg gccagtagcc attttgatac tcagcaggac
     1921 gagcggggct taagctaccg ttttaattac agcaaacaag tggatgccac taacagcacg
     1981 atttccctcg ccgcttatcg tttctccgat cgtcattttc acagctacgc caactatctg
     2041 gatcacaaat acaacgacag cgatgcgcag gacgaaaaac agacgatcag cttatctgtg
     2101 ggccaaccga ttaccccact aaacctcaat ctttacgcca acctgctaca tcaaacctgg
     2161 tggaatgcag acgcctccac gaccgccaac atcacggcag gttttaatgt tgatattggt
     2221 gactggagag atatctctat ttctacgtca ttcaatacaa cccattacga agataaagat
     2281 cgcgacaacc agatttacct gtcgatttcg ctccccttcg gtaacggtgg tcgggttggc
     2341 tatgacatgc aaaacagtag ccacagcacc acacaccgca tgtcgtggaa cgatacgctg
     2401 gatgaacgta atagctgggg catgtctgcc ggactgcaat ccgaccgtcc tgacaatgga
     2461 gcccaggtga gcggtaacta tcagcacctg agttcagcgg gtgagtggga tatttctggt
     2521 acctatgccg ccaatgatta cagttccgtc agcagcagct ggagcggttc tttcaccgca
     2581 acccaatatg gtgcagcgtt tcatcgccgc agctccacca atgaaccacg cctgatggtc
     2641 agcaccgatg gcgtggcaga tattccggtt cagggcaatc tcgactacac caaccatttt
     2701 ggcattgcgg tggtgccgtt gatttccagt taccagcctt ccaccgtggc ggtgaacatg
     2761 aatgacttac ccgacggcgt aacagttgca gaaaacgtta tcaaagaaac gtggattgaa
     2821 ggcgcgatag gttacaaatc actggcttcc cgttccggta aagacggtaa cgtcatcatt
     2881 cgcaacgcca gcggtcagtt tcctcccctc ggagcggata tccgccagga tgacagcggc
     2941 attagcgtgg ggatggttgg cgaggaagga catgcctggt taagcggagt cgctgaaaat
     3001 caaaagttta ccgtggtctg gggtgatagc cagcattgct cgctccatct tcctgaacat
     3061 atggaagaca ccgcaaatcg cctgatttta ccttgtcatt aattaaggaa atgaaaatga
     3121 catttatgaa aggactaccc cttttgttgt tggttgccag tctgtgcagc cacgctgcac
     3181 tacaacccga tcgcactcgt attgtgttta acgctaatga taaagctacc agtctgcggg
     3241 tggataatcg tagcgataaa ctcccctacc tcgcttattc ctggctcgaa aatgaaaaag
     3301 gggagaaaag cgatgatctt ctggtcgccc tgccgccgat acaacgtctg gaaccgaaag
     3361 caacaacaca ggtacggata gtaaagcaag cctcgaccac aaaattaccg ggcgatcgcg
     3421 aaacgctatt tttctacaat atgcgtgaaa ttcctcccgc cccggaaaaa aacagcgacc
     3481 atgccgttct gcaagtcgcc attcagagtc gtatcaaggt gttctggcga ccggctgcat
     3541 tacgcaagaa agcaggagaa aaggtcgaac tgcagttaca ggtcagccag cagggcaacc
     3601 aactgacact gaaaaatcct accgcgtatt atctgaccat tgcctacctg ggacgaaatg
     3661 aaaaaggcgt tctccccggc ttcaaaaccg tgatggttgc gccttttagt acggtcaaca
     3721 caaacaccgg aaattatagc ggcagccagt tttatctcgg ttatatggat gattacggcg
     3781 cgttacgcat gacgacgctc aactgcagcg gacaatgcca tttacaagcc gtggaggcga
     3841 agaaatgagt gctggcaagg gagtgttgct cgtcatctgt ctgttatttc tgtcgttgaa
     3901 gtctgcgctt gccctgaact gctactttgg ttcatccggt ggctcggtag aaaaatcaga
     3961 agcgattcaa ccttttgctg taccaggcaa tgccaaactc ggcgataaga tctgggaatc
     4021 tgacgatatt aaaataagat ctgggaatct gacgatatta aaattcccgt ctattgtgac
     4081 aacaatacca acggcaactt tgaaagcgaa catgtctacg tatgggtaaa cccctacccg
     4141 ggggtgcagg atcgttatta tcaactcgga gtaacttaca atggcgttga ttatgacgcc
     4201 aaccagggaa aaagccgcat cgacactaac cagtgtatcg acagtaagaa cattgacatt
     4261 tatacccctg atgggatggc agaacaaaat ttgctcgggt gatcccgcta atattcacat
     4321 gtcgcgaacc tttctcgctc gcatgcggtt atacgtcaaa atacgagaaa tgccgccgca
     4381 tgattatcaa agtacgctta gcgactatat cgtcgtgcaa tttgacggtg ccggtagcgt
     4441 taacgaagac cctactgccc aaaacctgaa atatcatatt actggtctgg aaaacattcg
     4501 cgtgctggat tgcagcgtca atttttccat ttcaccggaa acacagatga ttgattttgg
     4561 taaatttaat ttgctggata ttcgtcgcca cacaatgtcg aaaacattca gtattaagac
     4621 gacaaaatca caaaatgacc aatgcaccga cggatttaaa gtcagttcct ccttttatac
     4681 cgaagaaacg ttggttgaag aagataaagc attgttgatt ggtaatggtc tgaagctacg
     4741 tttgctggat gaaaacgcct cgccttatac cttcaataaa tatgctgaat acgccgattt
     4801 caccagcgac atgttggtct acgaaaaaac ctatacagct gaactttcgt ccattgcagg
     4861 tacccccatc gaggctgggc ccttcgatac agtggtgctt tttaaaatta actataactg
     4921 atttgccatg atgccctttg cagccggaaa gggcatctct ttcagaaaat tgctgatttt
     4981 ccccttttcc ttgattgctg ttattttttc ctagcccggc tttctttatg ctgtatcgat
     5041 acaccaaaga gaaaataatg agggagcgaa ggatgccagt tttgcagtgg ggaatgttat
     5101 gtgtgctatc acttctcctt tctattggtt tcctcgcggt acatctcccg gcggcgctat
     5161 tacttgggcc gatgatcgcc gggatcatct tcagtatgcg cggaattact ctgcaacttc
     5221 cccgctccgc ttttctcgcc gcgcaggcca ttcttggctg catgattgcg caaaacctca
     5281 ctggttcgat tctcaccacg ctggccgcca actggccgat cgtgcttgct attttactgg
     5341 tgacgctgct ttccagcgcc atcgtgggtt ggttattggt gcgctatagc tcactgcctg
     5401 gaaataccgg tgcctggggt tcttctccag gcggcgcagc ggcaatggtc gctatggcgc
     5461 aagattacgg cgcagatatt cgcctggtgg cgtttatgca atatctgcga gtgctgttcg
     5521 tcgcgggggc cgctgttctg gtcacccgca tgatgctggg tgataacgct gaagcggtta
     5581 accagcaaat cgtctggttc ccgccagtga gcattaatct cctgcttacg attttgctcg
     5641 cagtcgttgc cggtacggcg ggatgcatgc tgcgtcttcc ttccggcacg atgctcatcc
     5701 cgatgctggc gggcgcagtg ctccagtctg gtcagctcat caccatcgaa ctaccggaat
     5761 ggctgctggc gatggcgtat atggcaattg gctggcggat tggtcttggt ttcgataaac
     5821 aaatcttact gcgggcacta cgcccgctac cgcaaatcct gctgtcgatt tttgctctgc
     5881 tggctatttg tgcaggtatg gcgtggggac tgacccggtt tatgcatatt gattttatga
     5941 ctgcctacct cgccaccagt cccggcgggc ttgataccgt ggcggtcatc gccgcaggga
     6001 gcaatgccga tatggcgctt attatggcga tgcaaaccct gcgcctgttc agtattttgc
     6061 tgacggggcc agccattgcg cggtttattt caacctatgc gccgaagcgg tcggcctagt
     6121 gctggcagcc tggacaccag taaaacgggc gcgaggacaa cgtggttttc tcaatgatgc
     6181 cgccacagcg ttcgcacagt tcgccatcac gatgaaaaac cttaaagcga aacagcgccc
     6241 catgatgctt attctcatcc acctgccccc gcgtagcgta agaaaaccga ggaatctcca
     6301 gtaacgcgtg tgcgagtgca tccagttgcg ccgcattgag atctttcgct ttatgatttc
     6361 cagtcaaccc aacctgccag aggatctcca cccgcaaata attgccaagc ccagccagaa
     6421 acgcctgatc gagcagtaat cctgcaaact gacggttacg aaagcgcggc gacaataacc
     6481 gttcctttac cacctccggc gtcagattcg gatccagcac atcgggacca acgcgttgta
     6541 aaaacggatg cgtggtcagt tgttccggcc tcaacatctc aatatccgag gcgctataaa
     6601 gcagaatggt tttgtcagcc gtttgcagtt ttacccgcaa tactcgcgtg gtctgcggct
     6661 cttcgccggt atcaaccacg cgccagacgc cgtaaagctg attatggctg tagagcgtta
     6721 aaccgttggg aaaatgagtt aacaacgcct taccacgcgt ttccacatgg gtaacgtgtt
     6781 gaccgataag ttgtgattga taagttttta actgcgggaa ggcaaaccag acatcagtta
     6841 gtggtttgcc tttgatcgcc gcctccaggt tatccgctgc acggcggatc tccgggcctt
     6901 caggcatggt gatatccttt tttcattctt tattaagcgg ggttatgctg cgacaacaat
     6961 cccctttttg gcaaatgtag ctcgtaatcg gcgggcgaac gccagagcgt gctcgccgtc
     7021 gccatgcagg cagaccgttt gcgccgcgac cgttgcccat tcgccggtga tacttttgac
     7081 tctgccgtgt tgcaccatct ccagcgtttg cgccaacgcc tgttcttcgt tttcaatcaa
     7141 cgcgcctggc tgacttcgcg gcaccagcga gccgtcagcc tgataaccgc gatcggcaaa
     7201 cacttcctcg cgcgttgtca ggccatattg cttgcctgca cgaatcagct cgcttcctgc
     7261 cagcccaacg agaaccaatg ccggatcgca agcgtatacc gctctggcga tggcgtctgc
     7321 cagttgtgct tctttcgccg cctggttgta caacatgccg tgcggtttga catgacgcat
     7381 tacgccgcct tgcgcacggg caatcgttgc cagcgcgcca atttgataca gcgtctgggc
     7441 gtaaacggtt tccggcggca gctgcatggc gctgcgacca aaattttccc tgtcgggaaa
     7501 actcgggtga gcgccaatcg cgacaccatt ttttattgct tcacgcacgc aagcctgcat
     7561 ggtttgcgca tcgcctgcat gaaatccaca agcaatattg gcagaggaaa ccagcgttaa
     7621 tagctctgcg tcgctggcgc agccttcgcc cagatcggcg ttcaggtcaa ttttcattgt
     7681 gcagccgcca cgctaattgt tcaaaataac gttgctgatc ttgccgcgct ttcagtgcct
     7741 cttccagtga acactggaca aaatgaatcg gctgaccgag cggaatttgc gccagatggt
     7801 acatatcagc ctcaatgata caggcaatac gtggataacc accggtggtc tgtgcgtcgt
     7861 tcatcaacac aatcggctgc ccgttatgcg gcacctgcac cacgcccggt aacaaaccgt
     7921 gagataacag ttcgcgatcg gtgatgcgtt ttaaaatttg cccctgtaaa cgatagccca
     7981 ttcggttact ttgcgagcta agctgccagg gcgaacgcca gaatgcatcc tgcgaggcgc
     8041 gatcgaactc atgatattcc ggccccggca aggcgcgaat gcggttgccc cacagcagct
     8101 gtttaacgcc ctgcgcttcc ataaaatcac gcttcgattt gccaatcggg agtcagtcac
     8161 catccttcag taaacggcct tccagcccgc caatccccac tttgagatcg gtgctgcatg
     8221 accccattac cggcggaaca tcaataccac ccgcgaccgc cagataactg cgcatcccgt
     8281 gctgcgggcg tttaagcgtt aaacgctggc ctgctttcat cggcaatcgc cagccggtcc
     8341 agacggcgtt atcatccaac cgcgcttcgc aacctgcacc cgtcagggca aaccacccat
     8401 cagtttcgaa ctcaactgtt aactgaccga gcgtgatctc cagcgcgggg gcattggcgt
     8461 cattacccac cagcaggtta gcaatgcgca gcgcaggcat atccagtgcg ccacagtggc
     8521 tgatacccga ctggcaaaaa ccgtgacgac cgccatcctg cacagtggtg tacatgcccg
     8581 cacgaataat cttcagcata ttccctcctt ctgcggtaca aatcgcacgc tgtctcccgg
     8641 acgtaataag atgggttcgt cacgcgccgg atcaaacagg ctgagtgagg tatgaccaat
     8701 caactgccag ccacccggcg ttgccagcgg ataaacacca gtctgcggcc cgccgatccc
     8761 gacagaacct gccggaacga gcaagcgcgg ttcagcgcgc cgtggcgtgt gtagttgttc
     8821 cggcaaactc ccgagatacg ggaagcccgg ttgaaaacct aaaaaccaga ccacgtattc
     8881 cacggaggag tgcaattcaa caacctgttt ttcgctcaac ccgcaatacg ccgcgaccac
     8941 cgccaaatcc ggtccgcctg caccaccgta aaccaccgga atttcaataa agcgagactc
     9001 aggttccagg gcctcgctct cctcccacca gcgttgcaaa cgctctatgg catccagcgc
     9061 cagcgactca ggattacgca aaatcaccgt gatattgttc atgccgggaa tggcttcaac
     9121 cacattcggc atatccacca gacgctgcgc cagtcgccag atccgtttct ggctagccag
     9181 cgtcaccggc ggttccagtt ccagcactac cgccgtttca cctatcagat aacaacgcgc
     9241 tcgttgcact ttcgatccct ctgattattc gttatgcagg attaggaata tcaataaagg
     9301 tcacatcaag atcggtattt tcattcagcc actcgctcaa tgcgcgaata ccaccgcgtt
     9361 cagtggcatg gtgacctgca gcataaaaat gcaatccctg ctcgcgggct gaatgaatgg
     9421 tctgttcaga aacttcgcca gtaataaaag catccacgcc aaaacgcgcg gcgctatcga
     9481 taaaactttg cccgccgccc gtgcaccagg cgacgcgctg gaccacctca ggtccggtat
     9541 cgccacacca taacggctta cgtcccagac gcgcttcaat ccaggaggct agttccattc
     9601 ccggcacagg catggtcagc tctccccacg gcaccaacgg ctcaatttcg cccatgaccg
     9661 tgatccccag taacgccgcc agctgtgcgt tattgcccag ctcaggatgt gcgtcaagcg
     9721 gcaaatgcca gccatacagg ttgatatcat tcgccagcaa cgtttttaaa cggttacgct
     9781 tcattccgcg aatgaccgga gactctcctt tccagaagta gccatgatgc acaatgaccg
     9841 catccgcgcc cagacgcact gcctcatcga gcaaagcctg gctggcggtg acaccggtaa
     9901 caattttttg caccgtctct ttgccttcca cctgcaaacc attcggcgcg taatcactaa
     9961 tcgccgcgct gttcagtttt tcgttaatca gttgttccag ttcggtgttt ttcatcttcg
    10021 cgccttccgt attctgatag ctacaccgtt aacatagcgt tacatcgcct gttcgtcgat
    10081 agccaatctt ttgacatatt aagaaacaaa cccgacgcca aaagacgcaa attgtttata
    10141 tcatcacgct tcagctaatg tatatttatg caatcagaca gcatattgca taaataatca
    10201 gatctgtatc aaagttttaa ctttaaggaa acacaagcac gccgcgcaac tattacgtga
    10261 cgttgccgtc aattgtggaa ttattatgaa taaacacgca tcacaaccgc gcgctattta
    10321 ctatgtcgtt gcgctgcaaa tctgggaata ttttagcttt tacggcatgc gtgccctgct
    10381 gattctctat ctcaccaatc aactaaaata caacgatact cacgcctacg agttatttag
    10441 cgcctactgt tcgctggtgt atgtcacgcc aatcctcggt ggctttttgg cggataaagt
    10501 tctcggcaat cgcatggcgg tgatgctggg ggcgttgttg atggcgatcg gtcatgtggt
    10561 gctgggtgcc agtgagatcc atccgtcatt cctctatctg tccctggcga ttatcgtctg
    10621 cggctatggc ctgtttaaat ctaacgtaag ctgtctgctc ggcgagctgt atgagccaac
    10681 cgatccgcgt cgtgatggcg gtttctcgct gatgtatgcg gcgggtaacg tggggtctat
    10741 tatcgcacct atcgcctgtg gttacgccca ggaagagtac agctgggcga tgggctttgg
    10801 cctggcggcg gttggaatga tcgcgggtct ggtcattttc ttatgtggca atcgtcattt
    10861 cactcatacc cgcggcgtta acaaaaaagt actgcgtgcg acaaactttc tcctgccgaa
    10921 ctggggatgg ctgctggttc tgctggtggc aacgcctgca ctgattaccg tgctgttctg
    10981 gaaagagtgg tcggtatacg ccttaattgt cgcgactatt attggcctgg gtgtactggc
    11041 aaaaatttat cgcaaagcag aaaaccagaa acagcggaag gagctggggc tgattgtgac
    11101 gctcaccttc ttcagtatgt tgttctgggc cttcgcacaa cagggcggta gctcgattag
    11161 cctttatatc gaccgcttcg ttaaccgcga tatgtttggt tataccgttc cgaccgcgat
    11221 gttccagtcg attaatgcct tcgcagttat gctgtgcggt gtgttcctgg cgtgggtggt
    11281 taaagaaagc gtcgcaggta atcgtaccgt gcgcatctgg ggaaaatttg ctcttggcct
    11341 tggcctgatg agcgccggat tctgcattct gaccttaagc gcccgctggt ccgcaatgta
    11401 tggtcactct tctctgccac tgatggtgtt aggcctcgcg gtgatgggct ttgcggaact
    11461 gtttatcgac ccggttgcca tgtcgcaaat tacgcgcatt gaaattccgg gcgtgaccgg
    11521 cgtattaacc ggcatctaca tgctgctttc tggcgcgatc gcgaactatc tggcgggcgt
    11581 gattgccgat cagacatcgc aggcttcgtt tgatgcttcc ggggcgatca actactccat
    11641 caatgcatat attgaagtat ttgatcaaat tacctggggc gcactggcgt gtgtaggact
    11701 ggtactgatg atttggctgt atcaggcgct gaaattcaga aaccgcgcgc tggcgctgga
    11761 gtcataatag gccagcaccg atgcctgatg cgacgcttga cgcgtcttat caggcctacg
    11821 tatttcctgc aatttattga atttgcacaa atttgtaggc tggattaggc gttcacgccg
    11881 catccggcat ctgggctcga ttgcctgacg cgtctgttat ttccccttcc gcgccgcctc
    11941 atacgctgcc aacgtttgta ctctcgcctc tttgtgctcg actatcggtt gcggataatc
    12001 cagcttcaca cctgctttct gcgcccactt ccacggctca tgcaccgctt tccctggtac
    12061 atcgcgcagc tccggtaacc atcgacgaat aaactcgccc tcacggtcaa atttctcgcc
    12121 ctgagttatc ggattgaaaa tacgaaaata cggcgcagca tcagtacccg tagaagccgc
    12181 ccactgccag ccaccgttat tagctgccaa atcaccatca atcagctgcg acatgaaata
    12241 tcgctcgcct tcgcgccagt cgatcaacaa atctttcacc agaaaactgg ctgtaatcat
    12301 ccgtagccgg ttatgcatcc agccagtgct gttaagctga cgcatagcgg catcaataat
    12361 cgggtatccc gttttgcctt tctgccaggc ctgtaaatgt gcgggattgc tttgccactg
    12421 cacacgatcc gtccaggcaa taaatggacg atgtttacac aacgaggggt aatacgtcat
    12481 cagatgacgg tagaactcgc gccagatcag ctcactaagc cagacactac cggccccacc
    12541 gtccagcgcc tgcggctgtt ccgccaacaa gcgatgcaaa cactggcgag gcgataaccc
    12601 gcccgttgcc aagctggcgg acaaacggct ggtgccttcc actgccggaa aatctcgttg
    12661 ttgctcatac tctccagcac cgttctcgca aaactggcgt aattgcgcaa tcgccgcttt
    12721 ttcttccacc gggaaatgcg cagtatcgaa agactgacga ggataattca gcgtaatgga
    12781 tggcgcgggc tttatcgatc cgctactacg aacttttggt gcagcgacgc actccggcat
    12841 cccttcccgc agccgtttca gccaggcatt cttaaaaggc gtaaagactt tgtacatctc
    12901 gtgattgccg gtcatcaccg cccccggcgg caggatcacg ctgtcatcaa atccttcaca
    12961 caccacgtta cgcagcgctc tttcaacgtc cacatcccgc gctcgctcat tcacctcata
    13021 ctgatagtta taaaaaagat gagtaacgct gttttccgcg cacacctgtt taactatttc
    13081 gacactggcg acaaagtcat ccacttcacg gaacaataaa ggaatacctt tttccgccag
    13141 cgcgatttgt agcccattca gttgagcatt gataagttca gcctgacgcg gcgacatgtt
    13201 atgcgtctcc cacttgcgtg gtgtagcgat atacagcgcc agcacgcgtg cagacgaatt
    13261 gcggcaggcg gcagccagtg cgagattatc gtgcagacgt aaatcctggc gaaaccaggc
    13321 cagatgggta gtcataaaac tcctgaatgg cgcaagttaa tcacacgcgg ccaaagttcg
    13381 aaataacgct gccctgaaag ccaggcgtca ggataaagcg ccatatagtg tttgatacgc
    13441 atcagcggcg caagaagtgg ttgctcgcca cggcgataac tgtcgataag cgcagccagc
    13501 tgctggcgtt ctgtggaatc aatatgcggg cgaaaataac cctgaacgtg catcaaaacg
    13561 ttggtgtgat cgcgcgggtt ggcgggatga gaaagcaaaa caattacgcg ttggcggtac
    13621 tggttgtaaa aatcatccag attttgccac tgatgaatat cagccacaaa cgggccaagt
    13681 ttgcggtact cgggctgaga atgggccaga aagacgagtt tatagcggct gtgataatcg
    13741 agaagcgccc cgcgcgttaa gccatgttct ttcagtcgat gtaactcatg aagggcgaag
    13801 atacggatta gggtgctgtc atcaaatcgt tgtagattca ttactgctcc tgaacggtat
    13861 acaggagtaa gtgtaggcgt tggtaaatga tgtttgtgaa gttaatccgc tagcagggca
    13921 agattacgaa agcgcgctcc ccgcaagcaa ctaatcaagg tagttaatca tggctgaaca
    13981 cgatattacg ccagaagtca ctatttccaa aacacagcgc cgctataaag tcggctatgt
    14041 cagagcccgc caccaggacc gcaaaaccgg tatagcccgg tattattcac aacatcccag
    14101 tctgcacctt aagggcgact ggctgaaaga ggccggattt gataccggac ggggtgtcac
    14161 ggtgaagatt tcggaggggg gcctgaccat catcgctgac agtgacgagg tgcaggaact
    14221 gcgggatggg atgtttagtg tgctgaacga aagttaaata ttaaaaaagc tgggaagatt
    14281 taaaaccttc ctagcttttt attctgtctt attataatgg atactcatca tcttccgatg
    14341 aa
//
LOCUS       DAZPAB010000096        14283 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZPAB010000096 DAZPAB010000000
VERSION     DAZPAB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14283
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.096"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..275
                     /locus_tag="ACHUPG_002520"
     CDS             <1..275
                     /locus_tag="ACHUPG_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862098.1"
                     /translation="IHDGSNDIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITD
                     PEFQDVPIKEMFWNLQGDLNLTCWFHYKDGQWRVITYIFWPPGARF"
     gene            300..2513
                     /locus_tag="ACHUPG_002521"
     CDS             300..2513
                     /locus_tag="ACHUPG_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFR0862099.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            2536..2981
                     /locus_tag="ACHUPG_002522"
                     /pseudo
     CDS             2536..2981
                     /locus_tag="ACHUPG_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3003..6561
                     /locus_tag="ACHUPG_002523"
                     /pseudo
     CDS             3003..6561
                     /locus_tag="ACHUPG_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459289.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            6781..6984
                     /locus_tag="ACHUPG_002524"
     CDS             6781..6984
                     /locus_tag="ACHUPG_002524"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862100.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7002..7322
                     /locus_tag="ACHUPG_002525"
     CDS             7002..7322
                     /locus_tag="ACHUPG_002525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862101.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(7388..7620)
                     /locus_tag="ACHUPG_002526"
                     /pseudo
     CDS             complement(7388..7620)
                     /locus_tag="ACHUPG_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(7730..8500)
                     /gene="yafV"
                     /locus_tag="ACHUPG_002527"
     CDS             complement(7730..8500)
                     /gene="yafV"
                     /locus_tag="ACHUPG_002527"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR0862102.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            8681..9127
                     /gene="ivy"
                     /locus_tag="ACHUPG_002528"
     CDS             8681..9127
                     /gene="ivy"
                     /locus_tag="ACHUPG_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR0862103.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9170..11614)
                     /gene="fadE"
                     /locus_tag="ACHUPG_002529"
     CDS             complement(9170..11614)
                     /gene="fadE"
                     /locus_tag="ACHUPG_002529"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR0862104.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            11854..12432
                     /gene="lpcA"
                     /locus_tag="ACHUPG_002530"
     CDS             11854..12432
                     /gene="lpcA"
                     /locus_tag="ACHUPG_002530"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR0862105.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            12638..13405
                     /locus_tag="ACHUPG_002531"
     CDS             12638..13405
                     /locus_tag="ACHUPG_002531"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR0862106.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(13376..14116)
                     /gene="dpaA"
                     /locus_tag="ACHUPG_002532"
     CDS             complement(13376..14116)
                     /gene="dpaA"
                     /locus_tag="ACHUPG_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR0862107.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
BASE COUNT         3530 a         3621 c         3923 g         3209 t
ORIGIN      
        1 atatacatga tggaagtaat gacatctttt ttatgaagaa atatgggccg cctgctcatg
       61 agttcgtgag gccacgatat aaatttgttg gtgagtatta tatcggcttg aatcaaactt
      121 atcgcataac agatcctgaa tttcaagatg ttcctattaa ggaaatgttc tggaatttgc
      181 agggggattt gaatctgaca tgttggtttc attataaaga cgggcaatgg agagtgatta
      241 cttatatttt ttggccacca ggggctagat tttaatccgg tattaaagga gcaaatatca
      301 tgagttttgt atctacgggt aataaaccag ttggaaacgg tggacccgtc atcaccacac
      361 caccaatagc tggcgaaagt ggcggtatga gcacaggttc agctgtaacc gatgtatctg
      421 gtgccgccga ggagatggca gaacaggctg cggcggacct gtttggtgcg ctgccggaac
      481 cgtcaggtct ggttaaagcc gcagtagctg ctgcgcaggc tgccgctgca gcagcaggga
      541 tctcggatat ggctggtgca gttcaggatg cagcggcgtc gcttgcagcc ggtgctccgg
      601 gggcgcataa cgtcacagta agtggtagtg cggtgccgcc gcagatgctt ttgtttgcag
      661 gcatgaatgg ttcggaaggg cttgggaatc tgttttctta cacagtacag cttaaaacgc
      721 tggacgccct gaacctgggg tacgtttccc ccgctgctaa cctgccgctt cagctcatgg
      781 tgggcaaaga cctgtgcgtc agtatcgaac tggacggcgg cggaaaacgg tatatcagcg
      841 ggctggtgac ggcggcgagg gtggtcggcc atgagagccg ttcggtaacg tatgaattac
      901 gcattgagcc atggctgaag ctgctgaccc ataccagcga ctacaaagcg ttccagaaca
      961 aaaccgtggt ggatattctc gatgaagtac tggatgaata ttcctttccg gtggaaaagc
     1021 ggctggtgga aaattatcca ccccgcgcct ggcaggtgca gtatggtgaa accgacttcg
     1081 atttcatcca gcgcctgatg caggaatggg ggatttactg gtggtttgag cacagcgaga
     1141 acagccacac gctggtgctg gtggatgcca tcaatggtca taaagcgtgc cctgactcgc
     1201 cgctggtgga gtggcaccag gaagggctga aactggataa ggaatttatc cacaccatta
     1261 cggccagtga gaggctgcgt actggaaagt gggtgatgga cgattttgat tttatgaagc
     1321 ctcgttcgct gcttaaaagt accgtggcca gtccgcgtga taccggtcat gctgaatacg
     1381 agcattacga atggcctggt gattacttca ctacgggcga aggcgagatg ctgacgcgca
     1441 ttcgtatgga agcgcagcgc agcccaggca gccgggcaca tggggccggt catatccgta
     1501 ccctcatgac cgggtacacc tttacgttga tgaatcaccc gacggcagaa attaatcagg
     1561 aatatctgct ggtacagacc actctgtttt tacgggataa tgcgcagcac agcggtcagg
     1621 atcagcattt tacctatgtc accacgtttg aactgcaccc caccagcgaa gtttaccgcc
     1681 cacaacgaac cctgagcaaa ccccacacca aaggcccgca gagcgccatc gtcaccggcc
     1741 cggcggggca ggaaatctgg acggataaat atggccgggt gaaggtgcag tttggctggg
     1801 accgttacgg aaagaatgac gaaaacagtt cctgctggat ccgcgtcagc tatccgtggg
     1861 cgggtaaagg attcggcatg atccagatcc cgcgtattgg ccaggaagta ctggtggatt
     1921 tcaaaaacgg cgatccggat ttgcccatca tcgtggggcg cacctataac caggacacca
     1981 tgccgccgtg ggggctgccg ggcatggcgt cgcagagtgg gatattcagc cactcattgc
     2041 agggagggcc gactaacgga aatatgctgc gcttcgatga taaaacgggt gcggaagagg
     2101 tgaagttcca tgcggaaaaa gacctcaata ccacggtaaa aaacaatgaa acccacacgg
     2161 tcatggtcga tcgcataaaa accattgtca aaaatgaaac catcagcgtg ggagaagacc
     2221 gtaatgccac catcacaaaa aatgatggtc tttccgtcaa actggctcaa accatcaatg
     2281 tcggaacgac atatcgtctg gatgttggcg atcaatttac ccttcgttgc ggcaatgcag
     2341 cgcttgtatt acacaaggat ggctcgattg agttttgcgg aaaacaactg ttactgcaca
     2401 ccagcgatgt tatgcaactc attggcagtg gcattgatat gaatccggat ggcggcacag
     2461 ccatgactgc ggatgatatt gccccgttac ctacccccga cgtttctgaa taaaaaatat
     2521 aaaaggaatt tttctatgac cagcctttac cgtattcagg agggctgctt tgccctgcct
     2581 gaaacttttc ttgatcgcac cgtcaatatc ttcgtcccat cgggcaatga aagggcaact
     2641 cccagtctga atattttccg cgatacgctc aggccagatg aaaatctgac cacctatatt
     2701 gaccgccaga tagccttgat gaaaaaaaaa acctaggcca gcaccgggta ctttcgcggg
     2761 gaacggcact ggcgggaact ggcgaagctg ccatgacagg tgaacagatt ctcgcaactc
     2821 atcgttctgg aaaaacagaa gtgtaccagc gtcaggccgg gtttatcacc gggccagcaa
     2881 aggtactaat gttgacgctg accactcagc gtccttttga tgatcacacc gaccagctat
     2941 ggactgcatg gttgactagt tttcagcctg ctaaaagcta aaaaaaacag ggaggttttg
     3001 tcatgtatga agccgcacgg gtggatgacc ccatctacca caccagcgcc cttgccgggt
     3061 ttttgatagg ggccatcata ggtatcgcca ttattgccgt cgccagcttt gcttttttta
     3121 cctgcggctt tttggccggg ctgattttga gttttctggc tgatcagata gcttccggag
     3181 tcctgcaact gggtgaggct attgggcgct cgatacattc cacagtggga aaaattctga
     3241 ccggttctga taatgtcagc accaacagtc gcccggccgc ccgggccgta ctgagtacgg
     3301 tgatatgcga agaccattcc ccggaaatcc gcattgccca aggttccggc aacatctaca
     3361 tcaacagcca gctgcccgta aggatgacca cactgagtgt gatgcagtga ttgaggacgg
     3421 ctcccccaat gtctttctcg gcggcggtac gcagacggta ctggaaatca gcccggaaat
     3481 ccctgactgg ctgcgtcagg tggtggatgt gctgtatgtg gtcgccggaa tgcttggcgg
     3541 gctggctggc gcctggcgtc aggcggcaaa gatggggtcg aaatttggta caaaatgtgc
     3601 ggcaaaattt attggtggcg aaatggtcgg gatggccatc tctgacacag tcatgggatt
     3661 gttcagcaat ccggtagatg tcaccactgg gcaaaaaatt ttgctgccgg agacggattt
     3721 taccctgcca ggacgcctgc ctgtcacctg ttcgcgcttt tacgccagcc acctggaaac
     3781 tgaaggattg ctgggcagag gatggcggct gaactgggag ataaacctgc gtgaggatga
     3841 aacatatatc accttcatcg gtgtacaggg tcgggaactg agttatccga aagagatgct
     3901 gataccgggc caccagatat tcgacccgga agagcagttt tacctgagcc gtctgcatga
     3961 cggatgctat gtgctgcatt acaccgactg cagctattac gtgtttgatg aatttgatga
     4021 ccacggcgtc gcgccgttac tttttatgga gacgccgtac cggcagcgta ttgctttcgg
     4081 gcgtgagaac ggcaggctgg tgcgggtggc ctccagcagc ggacaccatc tgttattgca
     4141 ccgcacgatg acgcaagcag gggaacgcct gtcgcatatt gaacttttga agggcgggcg
     4201 tcccggtaac ctggtggaat atcgttacga cgataacggg caactgactg gcgtggtgaa
     4261 ccgggctggt gtgacggtgc gccagtttgc ctatgaaaat ggcctgatga cagagcatcg
     4321 caacgcgacg ggctttacct gtacctacca ctgggaagag attgaaggtt tcccgcgcgt
     4381 ggtggaacac acgaccagcg acggcgagga ttaccggttc cactatgatt ttgctggcgg
     4441 gcagacggtg gtaaccggca ggccggagca gaagtggcag tggtggtttg atgaagagac
     4501 gtatgtgact gcgcaccgga cgccgggcgg cggaatgtac cgcttcacgt acaacgaaaa
     4561 ccattttccg gtagcggtgg aactccccgg cgaacgcagg gtaacgctgg aatacgacac
     4621 cctgagccgg gtggtgaaag agacggaccc ggcaggccgg gtgacacaga cgcagtggaa
     4681 cggcagtttt gctgaaatca cgcgccgggc gctggatgac gaccacgtat ggaaggcgga
     4741 ttacaacgaa cacggccagg tgatacggga gacggacccg gaagggcgcg tcacgcggta
     4801 cgggtatgat gatcaggggc tgccggagac ggtctgccac cccggtaaac agcaggaccg
     4861 ttacacctgg aacgcgctgg ggttactgag cagccaccgt cgcatcacgg gcagtgtgca
     4921 gtcctggcag tatacgcagc gcgggatgct ggcgcgccat acggatgaag agaaacggga
     4981 gacgcgctgg cagtacaccc cggaagggct ggttgccagt ctgagcaacg gcaacggggc
     5041 tcagtaccgg ttcagctacg acggcgacgg acggctgacg ggagaacagc gcccggacgg
     5101 cctgataagg atgtttgcgc tgaatgcgga cgggtttccg gtcatcatcc cgacgcaggg
     5161 cacagagggc ggggtgcgca atgaacagca ggagcgtgat gcgctgggcc gcctgctgcg
     5221 cagtgacacg cagcacagta cacggacctt cagctacaac cgtcttgacc agataacgga
     5281 agtgacgctc acccccacgg aagaagggga acggctgcac cacatgcagg cggacacggt
     5341 gcgttttgcg tatgaccgca gcgggtggct gacggcagag cacagcgtgc acggcagtat
     5401 caaataccgg cgcgatgcgc tgggcaatcc caccgatatc acgttgccgg acgggcagca
     5461 tctcagtcat ctttactacg gcagcgggca tctgctccag acggcactgg acggtatcac
     5521 ggtcagtgaa tatgagcgtg acagcctgca ccgtcaggtg atacgcacgc aggggaaact
     5581 ggcgacgttc agcggctaca acgccgacaa ccggttaagc tggcagcgca gcctgccagg
     5641 tggcagccag aatccacagc aggcggtact cccccggcgc agaacgacag cgtgacggcg
     5701 cgggactata tctggaacgc agacggtgaa gtcggcggca tcaatgacaa actgcgcggt
     5761 tgcctggtgt tcagctatga ccgcagcggc tggctgacca gccgcacggg gcagatgtat
     5821 gaccatgacc attattatta cgacaaagcg ggcaacctgc tgacggatga gtatcaggga
     5881 gcggtgatgg ataaccgcct gccgggttac gggcgtgacc gctaccgcta taacgaatgg
     5941 ggcgaactga cagagcgcag ggaccagcag ctggagtgga acgcgcaggg ccagttaacg
     6001 cgggtcatca gcagcaacag cgagacgcgg tatcagtacg atgcgctggg caggcgcatc
     6061 agcaaggcga ccagcaatct ccataccgac cggggagaac gaagccggac aacgtttgtg
     6121 tgggaagggt tccggctgtt acaggagaca acgtggcagg gaaagcggac atacctgtac
     6181 gatgcggaac agccgtacac gccggtggct gccatcacgg ggcgcggaga gagccagaaa
     6241 atctggtatt accacacgga cctgacgggc acggtacacg aggtaaccgc gccggacggg
     6301 acgctggtgt gggcgggcta ccaggcggga tttggtgaaa accggggcga catcagcaac
     6361 agcggggcgt actttgagca gccgcagcgg ctgccggggc agtactttga cgaagaaaca
     6421 gggctgcatt acaatctgtt caggtattat gcgccggagt gcgggaggtt cgtaagccag
     6481 gacccgatag gattaaacgg tgggttgaac ttatatgctt atgcgcctaa tccgttaggt
     6541 tggatcgatc cgctaggact gaattcttta ggaatagatt caaataatat atttagagga
     6601 gatggtaact ataaaggtgg cggaattgga cagccaaaga gtggcacaat atcagcacag
     6661 gacattattg atcatctcta agaaaaaggt gatgggcgtt taacatcttt tagtactaaa
     6721 aaaacatcta gttccaactc tcgtggtgca gattttttct caaaaaaaca tgtcttcaaa
     6781 gtggcagttt ctgatctcga tgatttagtt aaaaataatc agatcactgt tttaacacaa
     6841 gaaaaagtaa aagcaatatt tggtttagaa tcaagcaaga aaattagaaa agaacttggt
     6901 aatattcttc agaatatggc taggaataat gaggtgttag ttcatgggca aattcttgga
     6961 aataaagtta gccgatgtaa ttagttagtg gagaggaagt tgtggacgaa attagaataa
     7021 gaattgctgg taatacggct gcgccttgtt tccatgtgct aaaaaataag ggatataaag
     7081 taacaatgga aactcatgca ttatctgacg attgtttaga ttgtgtatat gattggtatg
     7141 cggaaaaaaa tggacaattt tttagtgcaa cgagtccaga agagttactt ggattagttg
     7201 caatgtggga agttcgtggt aatgactgga gagctaattt gaatgagata aatacttatc
     7261 aaaaagccat agaagaatcc ccgttttttg ataaaaacgg caatgaaata gagtctgact
     7321 aaattttatt gatatagcaa cgataataac cggaaatttc ttccagatct ttccggcttt
     7381 ttattattta actttcgttc agcacactaa acatcccgtc tcgcatcccc ttaactggct
     7441 gtttaacctg ataaagttcc ctgcgcagtt cctgtacttc gttgttatcc caagcaggat
     7501 caaacatccc tccgagatct tcacggtaac gcccgtgccg atctcaaatc ccgcttttct
     7561 cagccatttg gcgctgattt tgatcaccgg cgtgtcgctg cggttgggca gataacccac
     7621 ggtacagcga cgctgtgttt tggaaatggt gacttctggc gtaatattgt gtttaagtta
     7681 ggggctttcg taatctagcc cagcccccgg ccctaaacac aacccccact caccacaacc
     7741 taaactcatc cgcatcccgc catgccggaa acttttctct atattcccgc aatgccacca
     7801 tcgacagctc cgcatcaata cgcgttgcct tatgcgcgtc ggcggtagcg ataatctcgc
     7861 cttgcggatt aatcacccgg ctgtcaccgc gataatggca gccgttgcca tcgctgccga
     7921 cgcgattgca tcccgccaca tacgcctgat tctcaatcgc gcgggccgtc agcaatgcct
     7981 gccagtgcag agagcgcgga gctggccagt tggcgacgta cagggcgagg tcataatcgt
     8041 tgagattgcg cgaccacact ggaaaacgta agtcgtagca caccagcggc aaaatacgcc
     8101 agccgcgcca ttccacaatc actcgcgcat tgcccgcttt ataatgcaga tgctcatctg
     8161 ccatgcggaa cagatgacgc ttatcataaa aatgtaccgt gccgcccggc tcaaccagca
     8221 aaaagcggtt aaccgaacca gactccgttt gtaatgcaac actgcctgca atcagcgcat
     8281 tgcactgctg cgccttagct gtcatccagt tcactacgtc atcttgtgca agcgacgaag
     8341 ctgccgcttc catggcaaag ccgctggtaa acatctccgg tagaacgatc acatcgcgcc
     8401 cggtaatacc ttccagttga cgatcaaaat ggcgcaggtt ggcaggacca tccacccaca
     8461 ccaacggttg ctgcaaaagc gtaatcttca aactaggcac ggtgtacaac tcctttatgc
     8521 gaagggtttt ataactttaa caccttatca ggcagttgcc ttagcgcaga ataaattgat
     8581 aacaaatgct gatattggaa atatctgatt tgcaaattat cgtgttatcg ctaggcttta
     8641 ggaggttaat aacatgggca ggataagctc gggaggaatg atgtttaagg caataacgac
     8701 agtcgccgca ctggtcatcg ccaccagtgc aatggcgcag gatgatttaa ccattagcag
     8761 ccttgcaaag ggcgaaacca ccaaagctgc gtttaatcag atggtgcaag ggcataagct
     8821 acctgcatgg gtgatgaaag gcggtactta tactcccgca caaaccgtaa cgttgggaga
     8881 tgagacgtat caggtgatga gcgcgtgcaa accgcatgac tgtggctcgc aacgtatcgc
     8941 tgtgatgtgg tccgagaaat ctaatcagat gacggggctg ttctcggcta ttgatgagaa
     9001 aacgtcgcaa gagaaactca cctggctgaa tgtgaacgat gcgctttcga ttgatggtaa
     9061 aacggtgctg ttcgcggcgt tgaccggcag cctggaaaac catccggatg gctttaattt
     9121 taaataatta gcggataaag aaacggagcc tttcggctcc gttattcaat tacgcggctt
     9181 caactttccg cactttctcc ggcaacttta ccggcttcgt cgccagctct tccggatcaa
     9241 agtcatcaac gttaatactg cgcagacggc tttcttccgc tttcaccaga atagcggctt
     9301 catctttatc aatcagcccc ttcgtcagcg cgttgtgcgc cagttcatcc agacgggtaa
     9361 atggcagatt tttacccagc tctttacaga tccgctgatg aatcgggtcg gcggcaatca
     9421 catccaccaa cgcctcttcc agtaaaccaa ccgggttatg ctcgcttggc gtcaggtact
     9481 gaccgcgacc aatacgtgaa cgagtggcgt tcggcacttg taaaatcttc gccattttat
     9541 gatccagctt gtcagacggt gccagataat gacgtccggt cgggaagatc accacattca
     9601 gcagcccggc aaccacgcgg ttcgggaagt tttgcagtaa attgtccatt gcctgttctg
     9661 cctgatacag cgcatcctgt acgccccagt gcaccagcgg cagatcggct tcattacggc
     9721 cttcgtcgtc ataacgcttc agaacggcag aggcgaggta aagctggctt aaaatatccc
     9781 ccagacgtgc cgagatacgc tcacgacgtt tcaggctgcc gcccagcagt gccatcgaga
     9841 catcagaaag cagggcgagg ttggcgctca ggcggttcag gtgctgatag tagcgtttag
     9901 tggcatcgcc ggttggcgtg ctgctggtta aaccgcgcgt caggcccagc cagaagctgc
     9961 gaactttgtt gctaccgacg tgaccgatat gtttgaacaa cagtttatcg aacgcgttga
    10021 cgtcattgtt cttcgccgct tccatctctt ccagcacgta cggatggcaa cgaatcgctc
    10081 cttgtccgaa gatcatcatg ctgcgggtca gaatgttagc cccttcaacg gtgatggcaa
    10141 tcggtgcgcc ctggtaagca cgcgccagga agttgctttg cccgagcata atgcctttac
    10201 cgccggtaat atccatcgca tcaataatcg actgctgccc gcggtgggta cagtgatact
    10261 taacgatagc cgacagcacg gcaggttttt cgccgagcat aatgccgtag gtaatcagcg
    10321 atgccgcagc atccattaca taagcgttcc cggcaatacg cgccagcggc tcttcaatcc
    10381 cttccatctt accgatagag attttgaact gacggcgaat gtgagaatac gcgcccgttg
    10441 ccagcgctac cgatttcacg ccgccggttg agttggaagg cagggtgatg ccgcggccta
    10501 ccgagaggca ctccaccagc atccgccagc cttgcccggc cattttcggc ccgccgatga
    10561 tgtaatcgat cggcacgaag acatctttac cgcgcgtcgg tccgttctgg aacggtacgt
    10621 tcagcgggaa gtggcgacga ccaatttcca cgcccggcgt ggtggttggg atcagcgcac
    10681 aggtaatgcc taaatcttct gcaccgccga gtaatttttc cgggtcggag agtttaaacg
    10741 ccagcccaag cacggtcgca atcggtgcca gcgtaatgta gcgtttgttc caggtcagac
    10801 gcatccccag cacctgctgg ccctgccatt cgcccatgca gacaatcccg gtgtccggaa
    10861 tcgcgcccgc atcggaaccc gcttccgggc tggtcagtgc aaagcagggg atctcctgac
    10921 cacgcgccag acgcggcaga tagtgatctt tctgctcgtc agtgccgtaa tgttgcaaca
    10981 gttcgcccgg gcctaatgag tttggcacgc cgacggtaat cgccaggatc ccgctcacgc
    11041 cagagagttt ttgcagcaca cgagactggg cataagctga gaactccagt ccgccgtact
    11101 cttttttgat aatcatcgcg aagaagcgat gctctttaag gtacgcccac aactccggtg
    11161 gcagatccgc cagctcatga gtaatctgga aatcattcgc catccggcag gcttcttcta
    11221 ccgggccgtc gagaaacgct tgctcttcag cggtcaggcg cggctgcgga tagttatgca
    11281 gctttttcca gtccggcttg ccctggaaca agtcgccctc ccaccaggtg gtgcccgcat
    11341 caatcgcttc tttctcagtg cgcgacatcg gcggcatcac cttacggaaa ccgcgaaata
    11401 ccggcgcgga aatcatcgac ttacgcatag gcgcaaagtt aaatggcacg aggataatgg
    11461 tcagaggcac cagtacccac gccgaccaca gaccagcaac gccgagggcg gctgtccagg
    11521 cgagcaaaat cagactgctg ataaataagc tcacgcggtg atagaacaac gcgccgagca
    11581 ggacaaccgt agcgagaata ctcaaaatca tcataacgaa aagcccctta cttgtaggag
    11641 gtctgaccac ttgtgatgat atggttgtag tggatgtaaa aacatttagc aatgtgttta
    11701 caatataatt acaacaaagc tcacattgtt gctgttttta tccgcacttc aggtcaaaaa
    11761 gtcctggtca tagcacctgc ccgtacttct cgcttttggc ggtatccggt acactgcatt
    11821 ttgtctatta catttatgct gaaggatatc ctcatgtacc aggatcttat tcgtaacgaa
    11881 ctgaacgaag cggcggaaac gctggctaac tttttaaaag atgacgccaa tattcacgcc
    11941 attcagcgcg cggcggtcct gttagcagac agctttaaag ccggtggcaa agtgctttcc
    12001 tgcggcaacg gcggttccca ttgcgacgct atgcactttg ccgaagagtt gaccggtcgc
    12061 taccgtgaaa accgtccggg ctacccggcg attgctattt ctgacgttag tcatatttcc
    12121 tgcgtcggta atgatttcgg tttcaatgat attttctccc gctacgttga agcggtaggt
    12181 cgcgaaggcg atgtactgct ggggatctcc acctccggta actctgcaaa cgtgatcaaa
    12241 gcgatcgcag cggcgcgtga gaaggggatg aaagtgatca ccctgaccgg taaagacggc
    12301 ggtaaaatgg ctggcacggc ggatatcgaa attcgcgttc cgcactttgg ttatgccgac
    12361 cgcattcagg agattcacat taaagtgatc catatcctga tccagttaat tgaaaaagag
    12421 atggttaagt aagtctggcg taggccggat aaggcgttta cgccgcatcc ggcacttgtg
    12481 cgctgatgcc tgatgcgatg ctgacgcgtc ttatcatgcc tacaaatctg tacccgaacc
    12541 gtaggccgaa taattcgttt acgccgcatc cgacctgaaa attcttaaat caatcttcgc
    12601 cggggtccat gcgctcccgc tgttgtggag gttacccatg tgcgaattgc tcgggatgag
    12661 cgccaacgtc cctaccgata tctgctttag tttcaccgga cttgtacagc gtggtggtgg
    12721 aaccgggcca cataaagatg gctggggcat taccttttac gaaggtaaag gctgtcgcac
    12781 atttaaagat ccacaaccca gctttaattc ccccatcgcc aaacttgtcc aggactaccc
    12841 gataaaatcc tgttcggtgg tggctcatat tcgccaggct aatcggggcg aggtggcgct
    12901 ggaaaatact cacccattta cccgcgagtt atgggggcgt aactggactt atgcccataa
    12961 cggacaactg acgggctaca aatcactgga aaccggcaac ttccgcccgg tcggtgaaac
    13021 cgacagcgaa aaagcctttt gctggctgct gcataaatta acgcagcgtt acccgcgcac
    13081 gccgggcaac atggcggcag tgtttaaata tatcgcctca ctggcggatg aactgcgggc
    13141 gaagggcgtt ttcaacatgc tgctttcgga cgggcgctat gtaatggcgt attgctcgac
    13201 taatttacac tggatcaccc gccgcgcgcc gtttggcgtg gcaacgttgc tggatcagga
    13261 tgtggaaatc gacttcagct cgcagaccac accgaatgat gtggtcacgg tgattgcgac
    13321 acagccgctg acgggcaatg aaacctggca aaaaattatg ccaggcgaat ggcgcttatt
    13381 ttgcctcggg gagcgtgtag tttgatgcca gttgtggctg caccacttcg tggcttaacg
    13441 gcttgctgac cacgtaacgg ccattggcca cagaaacggt cggtggctta cgggtttgct
    13501 caaagtagtc gtagcccggc ttcagctgct cccagaagtc cttaaagttg gaatatttat
    13561 ggcgcttcat attggcgtcg gtcatgcgga atgggtaaat actcacctgc acgctcggct
    13621 gaccaaacac cagcgcacca gtaacgaact ggaagatctc atcaataccc tgattggtca
    13681 ttgcgtagca gccgatggat acacaatcgc cgtggatcat caggtatttc ccttcataac
    13741 catgcgcacg gtcataagca ttggggaatc caatattaat cgctttgtag taacggctgt
    13801 ctggttttaa ctgattacgc tggacgctat aaaacccttc cgggcttttg aaatcgccct
    13861 gacgctgttt tggccctaag ccgccggaat atttacagat tttataactg tcgagcagtt
    13921 gatattgctc gcccattttg acgtaaagat cgagcgtacg ttcttccttg aagatctgga
    13981 tgtagacagg ggatcccatc aactgctgct tatactcttt gctcactggc gtggtcgagc
    14041 tactgctgcc cagcaaaccg gcaaacgaaa cgcacgggat caacaacatc gcaagaatta
    14101 atgcgatttt acgcatactg cttattcctt gataaaacgg ttacacacgc caggacggca
    14161 aaatggatcc caaatcggaa tagtctggat ttggaaggct cacattatca ccaaaagagt
    14221 tttacgcaag cctgtcggcg ctgggtttac aaatttagca caggggcagt tttcaatagt
    14281 ccg
//
LOCUS       DAZPAB010000097        14200 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZPAB010000097 DAZPAB010000000
VERSION     DAZPAB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.097"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..79
                     /locus_tag="ACHUPG_002533"
     CDS             <1..79
                     /locus_tag="ACHUPG_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862108.1"
                     /translation="RAFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACHUPG_002534"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="ACHUPG_002534"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR0862109.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="ACHUPG_002535"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="ACHUPG_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR0862110.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACHUPG_002536"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="ACHUPG_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR0862111.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACHUPG_002537"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="ACHUPG_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR0862112.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACHUPG_002538"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="ACHUPG_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR0862113.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="ACHUPG_002539"
     CDS             5377..5835
                     /locus_tag="ACHUPG_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR0862114.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="ACHUPG_002540"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="ACHUPG_002540"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR0862115.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="ACHUPG_002541"
     tRNA            6887..6971
                     /locus_tag="ACHUPG_002541"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="ACHUPG_002542"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="ACHUPG_002542"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACHUPG_002543"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="ACHUPG_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR0862116.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACHUPG_002544"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="ACHUPG_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR0862117.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACHUPG_002545"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="ACHUPG_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR0862118.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="ACHUPG_002546"
     CDS             complement(10252..13995)
                     /locus_tag="ACHUPG_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR0862119.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         3510 a         3752 c         3405 g         3533 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct
//
LOCUS       DAZPAB010000098        14110 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZPAB010000098 DAZPAB010000000
VERSION     DAZPAB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.098"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1..415
                     /locus_tag="ACHUPG_002547"
                     /pseudo
     CDS             1..415
                     /locus_tag="ACHUPG_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555244.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            580..4698
                     /gene="pic"
                     /locus_tag="ACHUPG_002548"
     CDS             580..4698
                     /gene="pic"
                     /locus_tag="ACHUPG_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HFR0862120.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4849..5091)
                     /locus_tag="ACHUPG_002549"
                     /pseudo
     CDS             complement(4849..5091)
                     /locus_tag="ACHUPG_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(5130..6080)
                     /locus_tag="ACHUPG_002550"
                     /pseudo
     CDS             complement(5130..6080)
                     /locus_tag="ACHUPG_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6131..6706)
                     /locus_tag="ACHUPG_002551"
                     /pseudo
     CDS             complement(6131..6706)
                     /locus_tag="ACHUPG_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6710..7110
                     /locus_tag="ACHUPG_002552"
                     /pseudo
     CDS             6710..7110
                     /locus_tag="ACHUPG_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7513..8094)
                     /locus_tag="ACHUPG_002553"
     CDS             complement(7513..8094)
                     /locus_tag="ACHUPG_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862121.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(8141..11998)
                     /gene="sigA"
                     /locus_tag="ACHUPG_002554"
     CDS             complement(8141..11998)
                     /gene="sigA"
                     /locus_tag="ACHUPG_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR0862122.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(12359..13363)
                     /locus_tag="ACHUPG_002555"
                     /pseudo
     CDS             complement(12359..13363)
                     /locus_tag="ACHUPG_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            13348..13725
                     /locus_tag="ACHUPG_002556"
                     /pseudo
     CDS             13348..13725
                     /locus_tag="ACHUPG_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13764..>14110)
                     /locus_tag="ACHUPG_002557"
     CDS             complement(13764..>14110)
                     /locus_tag="ACHUPG_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0862123.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         3541 a         3317 c         3459 g         3793 t
ORIGIN      
        1 catcgtgtgg tgacgagtct catcgtctat ggaaacgaca ttcgtgctgg atgcactgga
       61 gcaggcgttg tgggcccgtc gtccgtctgg caccatccat cacagcgata aaggctctca
      121 gtatgtgtca ctggcctata aggcgcgact aaaagaagca aaactgctgg catcaacagg
      181 gagtactggt gactcgtatg acaacgcgat ggcagagatc atcaagggcc tttacaaagc
      241 ggaggtaata caccgtaaga gctggaaaaa ccggacagaa gtggagctgg ccacactcac
      301 gtgggtggac tggtataaca atcgacgatt gctggaaagg ctgggccata tcccaccggc
      361 agaagcagaa aaagcttatt atgcttccat cggaaatgat gatctggcag cctgagttca
      421 cagataaaac actctccagg aaacccgggg cggttcagtt cacaaaaaca cattaatgca
      481 gtaactatat tttcctttct ggtgataacg tcgggttatc attagcttct tcagctattt
      541 tacttttata tcccttgtaa acatcatgga gaatccatag tgaataaagt ttattctctt
      601 aaatattgcc ccgtcaccgg ggggcttatt gctgtctctg aacttgcccg cagggtaata
      661 aaaaagacat gccgaagatt aacgcatatt cttctggctg gcattccagc aatctgtctg
      721 tgttactctc agatatccca ggcgggtatt gtccgttccg atattgccta tcagatttat
      781 cgtgatttcg ccgaaaacaa agggcttttt gtacctggtg ccaatgatat tccggtatat
      841 gataaggacg gaaaacttgt gggaagactg ggtaaagccc caatggccga tttcagcagt
      901 gtgagctcaa atggcgttgc tacgcttgta tcgcctcagt atatcgtcag cgtaaagcat
      961 aacggaggat atcggagtgt gagctttggt aatgggaaaa atacatattc ccttgttgac
     1021 cgtaataacc acccttctat tgacttccat gctccacgtc tgaataaact ggttacagaa
     1081 gttattccct cagcggtaac atcagaagga accaaagcca atgcttataa atacactgaa
     1141 cgttacaccg ctttttatcg ggtgggtagt ggtacgcagt acactaagga caaggacgga
     1201 aatttagtta aggttgccgg tggatatgct tttaaaacag gaggaaccac aggagttcct
     1261 ctgatatctg atgcaacaat agtctctaat cccgggcaaa cttataatcc tgtaaacggc
     1321 cctttacctg actatggagc ccctggggat agtggttctc ctttgtttgc ttatgataaa
     1381 caacaaaaaa aatgggttat tgttgctgta ttaagagcat atgcaggtat taatggtgct
     1441 acgaactggt ggaatgtcat accaacagat tatctgaacc aggttatgca ggacgatttc
     1501 gatgcccccg tagactttgt ttccggactg ggccccctga actggacata cgacaaaaca
     1561 tcaggcacag gtaccctgag ccagggcagt aaaaactgga ccatgcacgg gcagaaagac
     1621 aatgacctca atgccggtaa aaatctggta ttcagcgggc agaatggtgc aattatcctg
     1681 aaagacagtg tgactcaggg tgccggttat ctcgaattta aagacagtta caccgtatct
     1741 gctgaatccg gaaaaacatg gacgggtgcc ggcattatta ctgacaaggg gacgaatgta
     1801 acctggaagg tcaacggcgt tgccggtgac aacttgcata agctggggga aggaaccctg
     1861 accataaacg gaacaggtgt aaacccggga ggactgaaaa cgggagacgg tatcgttgta
     1921 cttaaccagc aggcagacac tgcaggtaat atccaggcct tcagttcagt gaacctcgcc
     1981 agcggacgtc cgaccgtggt gctcggggat gcccgtcagg tcaatccgga taacatttca
     2041 tggggatacc ggggaggtaa gcttgacctt aatggtaatg ccgttacctt cacccgactg
     2101 caggctgctg attacggggc ggtgattaca aataatgcac agcaaaaatc ccagctttta
     2161 ctggatctta aggctcagga tacaaatgtc agtgaaccga cgattggaaa tatatccccc
     2221 tttggtggta ccggcactcc aggaaacctg tacagcatga tactcaacag ccagacccgc
     2281 ttctatattc tgaaatctgc cagctatggt aacactctgt gggggaacag cctgaatgat
     2341 ccggctcagt gggagtttgt tggcatgaac aaaaacaaag cagttcagac agtaaaagat
     2401 aggatcctgg ccgggcgggc aaaacaaccc gttatctttc atggtcagct gaccgggaat
     2461 atggatgtcg ccattccaca ggtgccgggg ggaagaaagg taatctttga tggtagcgtg
     2521 aacctgccgg aaggtaccct gagtcaggac agtggcaccc tgatattcca gggacatccg
     2581 gttatccatg cctccatcag tggcagtgca ccggtcagcc tgaaccagaa agactgggaa
     2641 aaccgtcagt ttacaatgaa aacactgtcg ctgaaagacg ctgacttcca tctttcacgt
     2701 aacgcctcgc tgaacagtga cattaagtcg gataacagcc atatcacact gggaagtgac
     2761 agggcatttg tggataaaaa tgacggaaca ggaaattatg tcattccgga ggaaggtacc
     2821 tctgtcccgg acaccgtgaa tgacaggagc cagtatgaag ggaatattac gctgaaccat
     2881 aactcagccc tggatatcgg cagcaggttc accgggggga ttgacgctta tgacagtgcc
     2941 gtcagcatca cctctccgga cgtcctgttg acagccccgg gtgcttttgc cggcagttca
     3001 ctgacagtgc atgatggcgg tcatcttaca gcactgaacg gtcttttcag cgacgggcat
     3061 attcaggccg gtaagaacgg caaaatcacc ctgagcggta caccggttaa agatacggct
     3121 aatcagtatg cccctgctgt atatctgacg gacggatatg acctgaccgg cgataacgca
     3181 gcactggaaa ttacccgtgg agcacatgct tccggtgata ttcatgcctc tgcggcatca
     3241 acagttacca tcgggtctga cacgccggca gaactggctt ctgcggaaac ggctgcatcg
     3301 gcgtttgccg gcagtcttct tgagggctat aacgcagcat tcaatggtgc cataaccggt
     3361 ggcagggctg atgtcagtat gcataatgca ctgtggactc tgggtgggga ctctgccatc
     3421 cacagtctta ccgtcagaaa cagccgtatt agttctgaag gagaccgtac attccgtacc
     3481 ctgacggtga ataaactgga tgcaacaggc agtgattttg ttttgcgtac ggacctgaaa
     3541 aatgccgata aaattaatgt gactgaaaaa gccactggtt cagataacag cctgaacgtc
     3601 agctttatga ataatcctgc tcagggacag gccctgaata ttcctctggt cacggcaccg
     3661 gcgggaactt cagcagagat gtttaaggcc ggcacccggg tgacaggttt cagtcgggtg
     3721 accccaaccc tgcatgttga taccagtggt ggcaatacga agtggatact ggatggtttt
     3781 aaagcggagg ctgataaagc cgctgccgcg aaggctgaca gtttcatgaa tgccgggtat
     3841 aaaaacttca tgacggaagt taacaatctg aacaaacgta tgggtgacct gcgtgacaca
     3901 aacggtgatg ccggtgcctg ggcgcgcatc atgagtggtg ccggttctgc agacggtggt
     3961 tacagtgata attacaccca tgttcaggtc ggctttgaca aaaaacatga actggacggt
     4021 gtggacctgt ttaccggtgt cacgatgacc tataccgaca gcagtgcaga cagccatgca
     4081 ttcagcggaa agacgaaatc ggtggggggc ggtctgtatg cttcagcatt gtttgagtcc
     4141 ggtgcctata tcgatttgat tggtaaatat attcaccatg acaatgatta cacaggtaac
     4201 tttgctagcc tgggaacgaa acactacaac acccattcct ggtatgccgg tgctgaaacg
     4261 ggttaccgct atcacctgac agaggacacg ttcattgagc cgcaggctga actggtttac
     4321 ggcgccgtgt ccgggaaaac attccgctgg aaagacggtg atatggacct gagcatgaag
     4381 aacagggact tcagtccgct ggttggaaga acaggggttg aactgggcaa gaccttcagt
     4441 ggtaaggact ggagtgtgac ggcccgtgcc ggaaccagct ggcagtttga cctgctgaat
     4501 aatggagaga ccgtactgcg tgatgcgtcc ggggagaaac ggataaaagg agagaaggac
     4561 agccggatgc tgtttaatgt tggtatgaat gcgcagataa aggacaatat gcgctttggt
     4621 ctggagtttg agaagtcagc ctttggtaaa tataacgtgg ataatgcggt aaacgcgaat
     4681 ttccggtata tgttctgatg tgggggtaaa gggctgccgg aaggcagcct ttctcagaga
     4741 ctcttaaaat ataaaactga aggtcttcgg taagggggag tgtattagac gtcaactagt
     4801 tttgccgact ggcgcgagcc tgtttaagat tctgtgtaaa tgcctttctc agaagtgacc
     4861 gtccaggcgg tcaccgaact cgataataaa gcggctcatt gccattcgcc agtccttcaa
     4921 cggcatcgtc catttctggg acgcagactg gattgccagc cacaccactt ttttcaccga
     4981 gtcgtctgtc gggaacactt tacgcttttt gatcgcatgg cggatcacgc tgtttagcga
     5041 ctcgatggca ttcgtcgtat agatcacttt gcggatgtcc gttggataag cggtaagcgt
     5101 acagcgaggg ccgtattgac ggggatgtgt tattcagctg gcagtgctat gcgccacgga
     5161 agcagttcgc tgacccggtt gaccggccag tctgctatga cgccaagcac atggcgaagg
     5221 tagctttctg gatccacgtc attcagtttg cacgtcccga tcaggctgta cagtagcgcg
     5281 ccccgctcac caccatggtc agagccgaag aacaggaagt ttttacgacc cagactgacc
     5341 gcccgcaggg cattttcagc gatgttgttg tcgatttcca cccagccatt actgcagtac
     5401 acgttcaggg ccttccactg tttcagtagg tatgcgaacg cttttgccgt atccgagtga
     5461 cgcgacagtg ttttcatctg ttgctgtatc cggtcataca gtgactgcat cagtggcgcg
     5521 gctcttgctt ttcttgccgc cagacgctgt tctgctgaac atccccggac ttctgcctcg
     5581 atggtataca gttcaccgat acgctgcagg gcttccgtgg tgatgtcggt tggcactctt
     5641 gcatgcacat cgtggatttt tctccgggca tgggccatac acgcagcttc cgttattctg
     5701 ccggattcgt ataacgcccg gtaaccaccg taagcatctg cctgaagcac accgctgtaa
     5761 ccggctaggt gattctgtgg atggatacct ttccggtccg ggctgtacgc gaaccagacc
     5821 gccgggggca tctgtgaacc ggcgttacgg tcatcacgga catagaccca cagccgggca
     5881 gttcttgttt taccactgcc cggctcctgg accggaacgg ggatatcatc cgcatgaact
     5941 ttaccgggca tcagtacata ctggcgcagg acgtcataca gcggctccag cagttcagca
     6001 acggcaccgg tccagcgacc cagtgtggca cggctcagct ccacgccctg acgacggtat
     6061 atttctgact ggcggtataa ctgtcaacga cggatgaaaa gtggctcttc cgcaacctgg
     6121 gtggagattt tcatgccagt ggactcatta ccctctgcct gagtagttca aatcctgctc
     6181 gaccatacat ctggcgtttc agcatcttct tcaggcgatt tacatgacct tcgacaacac
     6241 cattactcca gcgactactg attgcctcac atatagctgc agcatcagct tccatccccg
     6301 ccgccacgcg ccgaaattct gctgaaccgc tttcgtggac tcgagtgaac caactgctta
     6361 gctgtgattt attttgggtt ttcagaatac ggtagaactc gagtgccagt tgctgcgcta
     6421 ttttcagctc aggttctttt tcacacatca gactaataaa tcgggaagca taattttctt
     6481 catccctggt tattctccag ggcatcaacc agcggcttac ccgggagact gaaggaagtc
     6541 tgacggcagt agtaaccggt gggttccagc ctttacgcca tttggcaacc gcatccctga
     6601 cgatggtttc actgcctgta aatccccggg ccaccatttc ccgccatatc cggctggcgt
     6661 tataattacc actttctcgt tgctctttta accactccct ccatggtgtc agcgaaaaac
     6721 gaaaccgctg ttgaaatccc tggaaagctg gttgcggaaa agatgaagac ccagtcgcga
     6781 cactcagagt tggcgaaggc gttcgcgtac gcacttaacc agtggccggc actgacgtac
     6841 tatgcgaacg atggctgggt ggaaatcgac aacaacatcg ctgaaaatgc cttgcgggcg
     6901 gtcagtctgg gtcgtaaaaa cttcctgttc ttcggctctg accatggtgg tgagcgggga
     6961 gcgctactgt acagcctgat cgggacgtgc aaactgaatg acgtggatcc agaaagctac
     7021 cttcgccatg tgcttggcgt catagcagac tggccggtca accgggtcag cgaactgctt
     7081 ccgtggcgca tagcactgcc agctgaataa cacatccccg tcaatacggc cctcgctgta
     7141 cgcttactca tcacctgcta ttaatccccg agtcagtact tcgcggcctg acgcgatcac
     7201 cctactcagt acttcagagc ccgcctccgg tacctgcctg gcgatcgccc ctgtgcaaca
     7261 aaataaatca accgttatca aaccacgatc gttcgtatgc gccgccgatg acgcgcgcct
     7321 gaaaaacgcg tcaatatgag ttccccggaa gcttacgata atacgcttat gaacattaca
     7381 acacgctcat gcggaacctg gatgggggaa ggaacggtga agctgttagc gaatgaccgg
     7441 cgtttataca aacgccggtc atgatgaatg atatttcaga atatacctcg ggctaaaata
     7501 ccgggacaga gatcaggcgt gattaatctg cagcattttt aataacagaa gtactgagct
     7561 tactttacct ttgatgatgt aatagttatt tccgtcggta ctcatttttc cctgaaataa
     7621 tgtcacggat gtttcttcct gctgatcctg caggcgcagt cgtaaggtca tatttttaat
     7681 gagctgagat ccgggtggca aggggtattt gctgtcagat actgggcgac tgagtctgat
     7741 gtggcgcgta tacagcccgt tttcgtacag ggtggcctct gttatggtgt cagtgaccag
     7801 catgtgggag acaatgctgt attctttttt aaagcaccca ccagaaacgg tgattgtaat
     7861 gataatggtc ttctcgatcg agatgtaaat acccgtttcg ttatactgtc cgtatcggaa
     7921 ccatgagaac tcagttgcat tccagccatg ctccagaata tgtgcatttt tttgcctgat
     7981 agtttctttt tttattcgct catgtttaag gcataacagg cagataaata atgtcagaaa
     8041 aagaaagcta ataatgagag caagagcgat atagtctgtc acagggtaat acatattctc
     8101 tccggtagaa gaagggccgc aaacgcggcc cgggctgtta tcagaaagag taacggaagt
     8161 tggcgttgat cgcgttatcc acgttgtatt taccaaatgc cgatttctca aactcaagac
     8221 cgaagcgaag attatcgcga atttcggcgt tgagaccaac attcatgaga atacgaccgt
     8281 ctttttcacc tttgatacgt ttctcaccgg acgcatcacg cagtacggtt tcaccgttgg
     8341 caaacaggtc aaactggtag ccaaggccgg cgcgggctgt gactttccag tccttaccgg
     8401 agaaggattt acccacatca acaccggtac gcccaatcag cggattaaag tccttatcct
     8461 tcatggtcac gctcattccg cgatcctgcc agtcgaaccg tttacctgat acggccccat
     8521 aaaccagttc tgcctgcggc tcaatccagg agtcttctgt tacatgatag cggtagcctg
     8581 cttcagcacc cacatacaag gaatgagaac tgtagtcttt tgttccgagt ccagcaaagg
     8641 tcgccgagta ctcattatca tggtgaacat acttactaat caggtcgata taggcaccag
     8701 agtcaaatat agcggaagca tacagacctg ccccgacgga cttcgtcttg ccactgaatg
     8761 cattactgct ggcatgactg tcggtatacg tcatagtcag accagtgaaa aggtcaagtc
     8821 catccagctc atgtttttta tccacaccaa tctgcacatg ggtgtagttg tcactgtatc
     8881 caccagaagc tgaacctgct ccgctcatga tgcgggccca tgcaccggcc tcgccattgg
     8941 tgtcacgcag atcccccatt cgtttattca ggttgttgac ttctgtcagg aaggctttgt
     9001 aattaccgga catatacgat tttgctgcac cggaagatgc tgtattttca gccaccttat
     9061 atccggtcag ggtccacgtg gtatgttcct gatcctcttt cgtgctgatc acaggagata
     9121 tgttgctgaa acctatggcc tgagtactgg ctttaaatat ctctttattg cttccttttg
     9181 gggctgttat caggtcaacg tttaatcctg agttattatt tcccactttc tgtatatcga
     9241 ccgacagact gttattgctt ccggttaact gatttttaac aatgagttta tccgattgat
     9301 ttacagactg gctcagatca gcccgcataa tcacagcagt attagtggca tccagtgtgt
     9361 ttactgtcac agtatggaaa cccgaattct ctatgttact aaacaacaaa agactgtcgc
     9421 gggtttttaa tgattgcagg gctgactgtc cgtttactct ccataaggtg ttatacatgc
     9481 tggctgaggc tgcaggagca tcaatacctc cggtataaga agtatcaaag ccacccagca
     9541 aacctgtagc cagcggtgtg tgtgtggttt tgtccaggtt atcttcttta tactggtcaa
     9601 caccaaacag aatagatgct tttttgtctg attttatatt accattgacc caggcataat
     9661 tttgagcctt taacgtggca ccatcttctg tcatattata tccatctgac atatagattg
     9721 ccgggctgta tttattattt tctgtagggg tgcctgttaa tgtcagggtt ccggccagtt
     9781 caatagcttt atctacatac atttttccct gggcggtaac agtagctcct ttgttgataa
     9841 tcaggtctgt attaattaag gataatgcac cagtcatagt aacagttgat ttatcaccaa
     9901 cagttatttt gctgttttga gcactcagac tgccgttgaa actactttcg cctgctgaat
     9961 ccccggagcg aagctgttgt ctgaatccaa aaccttcgcc agtaatgttt cttcctgaga
    10021 acatgtcaat gtatgcagtt ttatttccca gaacaatttg agagttttta gcctctatgt
    10081 gtccttttac attagcatcc cggcctatac tcagggttgc gttttctaaa tttaatttcc
    10141 ggaaattaaa tttacgactt tcccagtcgg gctgggaaaa agaggctatc tgattgttgg
    10201 acttatattc cgtattgtaa cgtttattta cagtgctttc ctggtccttt atggcagcag
    10261 aatagtcttt ttgacataaa aaaggaattg gacagttgtt attgccttct ttaaatatgg
    10321 catgttctgt cgcatgtcct tgtaatgtta actgtgcatt ccggacagag atatcgtttt
    10381 tgatatcgac tgagccatca aagatcagat tggcattatt gttatgttgc tgggtagtat
    10441 tgataatatg atttatgctt atattacctg atacattccc atggtacata tatgcatcag
    10501 tattagtaag tgataatgtt gattgcttca cgtttgagtt cgttacggtt gttcccgcat
    10561 ctgttgctgc gattttctga aatgactggt catagccgtt tagatccagt gttccgccat
    10621 ttctggtaaa atagatttcg ccattaccgc tttcgcttaa agcgtctttg gcatttattt
    10681 ttaccgtacc tttacctccg gccatgtaaa ttttattgaa ggctttttca gcactaagga
    10741 tgacagtccc attacctatt ttaaggttat ttccctgtgc tatttttaca tccagagtac
    10801 cactacctat tttatgcaga ttatctccgg caacgccctt aacattccat tcaacggtgg
    10861 tattattatc aatatctatc ccggcacctt tataagactt atcttttccg tacactttat
    10921 atgtctggtt cttatcgaat ataagcccgc caatgccaag atcggtatcc tgcttgagcg
    10981 ttaatacccc tcctcctgag acagacaggt ccttatcttt tataatcgat gataactctt
    11041 gttttttacc atccagctgc atagtgtttg tgaaattctt ttttaaattg tttaccagaa
    11101 tattattaaa taacgagtag tttgtccagg ctatattggt actactgata aaaccaactg
    11161 tactggtaac accatggaaa acccattttt gttgttcttt atcatataga taagaaccag
    11221 aaccactatc gccaaaaatt ggtgagtttt taaaaggggt tctgttggta taaatgtaac
    11281 tcttgttttt ccagtccaga ttaaaaagac ttgcgcttaa caagtcggga ttatagtttt
    11341 gtccaaacag atattgtttg ccgtttaata tcgttgaggt tgttccggca cctgctctga
    11401 agccaattat ttgttcctta ccgttgtagt ttacgccata tcttttcatc gcatcttcgg
    11461 gagaaagtga gaagtcggca ctctccgtaa cccctgttgt ttccacgacg aatttattca
    11521 gacgatgaac cgaaaagtcg ccagatgata ctctgttact tgctttatat gctgtctgat
    11581 cccatgactg tgttgttatt gcatgatgct gtgtaccgtt atgagtcgca gtaacactgt
    11641 acgcacctcc aattgatgtt gttgcacctt tgttggaaac agcagaaaaa tcaggtacag
    11701 gtagttctgg aaaatgaaat atctttccat ctttcatcat taattgaaca tttgttgcac
    11761 ccggtctgaa ctctcctctg ttttgagcaa ggtcaagata atcgcgggtc catatattat
    11821 ttatatccag cattgctgca aatgctgttt cgccataact tccatatatt gaggataaaa
    11881 taataccgag gataactttc tttcttgatg taccgacact aactttccgg gtcagttcag
    11941 aaacagcaac taatccacct gtaatatgac tatatttcag tgaataaatt ttattcatac
    12001 gtgaactccg atatttctga gttgtggtta tgggatagca tttaattaca acggggaact
    12061 atcagtcatg gtacgtaact gataagggta taatgataat cactgtcagt tatatttgta
    12121 tgacagatgt aacaaaatga aaaaaaagat aagcacaagt gattgattgg ttagatattg
    12181 atcacttggt gtttgtattt tgaattttgc gatcaaaatt tgatctttgt tgtttccggt
    12241 caatccttta caggttttct gtattatccg ggggagttaa gtctgtcgat gtccgacaga
    12301 cttttgatga tggaaacaac atgcagcgat aatgtgcatg ctgttttgca ggtggaactc
    12361 agaaggtgaa ctggaactgt gcgaccgcac cgtaagtgtt gtccgtgccg atcaggcctg
    12421 caacatcaag gtgaaccaca tcaaacggtg agatccccac cccggccgtg aatgcactgc
    12481 cgtcgttacc ggccagattc tgacggtaac cggcgcgcag ctgtgcccat ttccaggcat
    12541 taaattctgt gccaatggcg gcaaactgac ggttgctgtc ggaggtgaaa ccgctggccg
    12601 gggtcagatc cacatcaaat gcagtggtga acatcgcgtt gtgccaggag acaccggccg
    12661 tcacctgcgg gcggatcctg aaggtttccg tgataccgtt cacttctttt gtctcaatac
    12721 tacgggagat caggttctgt gcgaccagtc ccagcgtcca gttgtcatcc aggtccgtac
    12781 tggcaccgat gtcggcgttg atgccatttt tcgtgttgtg gtagcggtcg cctttgaagg
    12841 cgctgctgtc ataatttctg accagcacgt tgtagttgaa caggtcgacg cgctggaatt
    12901 tgggggggaa gcccagagac catttctgcc cggccgtttc cagttccgta gccagagcaa
    12961 tacccacatc agtgatcagt gccgtgcggg cacaggcctg tgatgtcagg cggcttttgt
    13021 ccacctcctg cccggtgctg tttgccacct cttccaggta agtgaggtca gcatcagaga
    13081 ctttaccgtc gacactgacg gtgccgtgtg atttaaccat cagtgcggca gagaggcgat
    13141 caccgggaag ggccgccaca gcagaaacgc ccagctgtgc ggcggcatgt gtgttacgga
    13201 agtcccggag gtgttctttc aggcgtgcgg cagcctcagg aacaccgtgg ttactttcga
    13261 tggcgcggtc gaaggccttc cactcatcac tgatccggtc ggcgttgtcg gtgatattat
    13321 ccggattgga gagctgcgcg ccgacggacg gaagcaggat gctgggctca tgcgcgacgc
    13381 aaaatccacg atgtatatat cagtactaaa agcgcgacgg cggaagaagc tctgaaacta
    13441 atcggtgaac tgtacgccat agagcacgat atacgcgggt taccggtgtc tgaacgcctg
    13501 gcggtcaggc aaatgcagag tagaccgctc ctgacttccc tgtataagct gatgcaggag
    13561 aaagaacaca cgttatcgaa gaaatgtcgt ctgggagatg cgttccggta tatcaggaag
    13621 cactgggctg cgttgtgcaa cttctgtgat gatggcctgg ctgaggtaga taataatgca
    13681 gcagaaagag cgcttcgtgc agtctgtctc gggaagaaaa ctttttcgag gatcactcat
    13741 tccgtgtctg actgcttcag ggtttaattt tgcttctgct gactgaatga tgattgggca
    13801 gcaagtgcaa tatcccacat aaatccagtc aactcacgag ccaccgccgt aatggctact
    13861 tgtgattttt tcccttttgc tgtcagagtt cggtaacgag aacacaatcg ttgttgagct
    13921 ttccatgcaa tatctttgga atattgtgtt acagagtccg gtcggtaata tataagccat
    13981 gctccaactt ttgcaggtgt acgataagac caggcggctt cgtaaagcag acgtctcagt
    14041 tcgctgtttc caacttttgt tattcctctg ggacgaatac tgtttccact ggaatattca
    14101 cctggcacca
//
LOCUS       DAZPAB010000099        13558 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZPAB010000099 DAZPAB010000000
VERSION     DAZPAB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.099"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            112..1167
                     /gene="phoE"
                     /locus_tag="ACHUPG_002558"
     CDS             112..1167
                     /gene="phoE"
                     /locus_tag="ACHUPG_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR0862124.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            complement(1206..1607)
                     /gene="crl"
                     /locus_tag="ACHUPG_002559"
     CDS             complement(1206..1607)
                     /gene="crl"
                     /locus_tag="ACHUPG_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR0862125.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="ACHUPG_002560"
     CDS             complement(1665..2909)
                     /gene="frsA"
                     /locus_tag="ACHUPG_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR0862126.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="ACHUPG_002561"
     CDS             complement(3001..3459)
                     /gene="gpt"
                     /locus_tag="ACHUPG_002561"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR0862127.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3721..5178
                     /gene="pepD"
                     /locus_tag="ACHUPG_002562"
     CDS             3721..5178
                     /gene="pepD"
                     /locus_tag="ACHUPG_002562"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR0862128.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5235..5849)
                     /gene="prfH"
                     /locus_tag="ACHUPG_002563"
                     /pseudo
     CDS             complement(5235..5849)
                     /gene="prfH"
                     /locus_tag="ACHUPG_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5911..6954)
                     /locus_tag="ACHUPG_002564"
     CDS             complement(5911..6954)
                     /locus_tag="ACHUPG_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR0862129.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            complement(7200..7652)
                     /locus_tag="ACHUPG_002565"
     CDS             complement(7200..7652)
                     /locus_tag="ACHUPG_002565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0862130.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="ACHUPG_002566"
     CDS             complement(7662..8060)
                     /gene="yafO"
                     /locus_tag="ACHUPG_002566"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFR0862131.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="ACHUPG_002567"
     CDS             complement(8063..8356)
                     /gene="yafN"
                     /locus_tag="ACHUPG_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFR0862132.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="ACHUPG_002568"
     CDS             complement(8408..9463)
                     /gene="dinB"
                     /locus_tag="ACHUPG_002568"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR0862133.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRECRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="ACHUPG_002569"
     CDS             complement(9534..10319)
                     /gene="lafU"
                     /locus_tag="ACHUPG_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR0862134.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            10291..12003
                     /locus_tag="ACHUPG_002570"
                     /pseudo
     CDS             10291..12003
                     /locus_tag="ACHUPG_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(12220..12717)
                     /gene="rayT"
                     /locus_tag="ACHUPG_002571"
     CDS             complement(12220..12717)
                     /gene="rayT"
                     /locus_tag="ACHUPG_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFR0862135.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(12893..13414)
                     /locus_tag="ACHUPG_002572"
                     /pseudo
     CDS             complement(12893..13414)
                     /locus_tag="ACHUPG_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
BASE COUNT         3376 a         3517 c         3393 g         3272 t
ORIGIN      
        1 aatatcatta atctgtaata tatctttaac aatctcaggt taaaaacttt cctgttttca
       61 acggggctct cccgctgaat attcgcgcgt taattaaaat caggaatgaa aatgaaaaag
      121 agcactctgg cattagtggt gatgggcatt gtggcatctg catctgtaca ggctgcagaa
      181 atatataata aagacggtaa taaactggat gtctatggca aagttaaagc catgcattat
      241 atgagtgata acgccagtaa agatggcgac cagagttata tccgttttgg ttttaaaggt
      301 gaaactcaaa ttaacgatca actgactggt tatggtcggt gggaagcgga gtttgccgga
      361 aataaagcgg agagtgatac tgcacagcaa aaaacgcgtc tcgcttttgc cgggttgaaa
      421 tataaagatt tgggttcttt cgattatggt cgtaacctgg gcgcgttgta tgacgtggaa
      481 gcctggaccg atatgttccc ggaatttggt ggcgattcct cggcgcagac cgacaacttt
      541 atgaccaaac gcgccagcgg tctggcgacg tatcggaaca ccgacttctt cggcgttatc
      601 gatggcctga acttaaccct gcaatatcaa gggaaaaacg aaaaccgcga cgttaaaaag
      661 caaaacggcg atggcttcgg cacgtcattg acatatgact ttggcggcag cgatttcgcc
      721 attagtgggg cctataccaa ctcagatcgc accaacgagc agaacctgca aagccgtggc
      781 acaggcaagc gtgcagaagc atgggcaaca ggtctgaaat acgatgccaa taatatttat
      841 ctggcaactt tctattctga aacacgcaaa atgacgccaa taactggcgg ctttgccaat
      901 aagacacaga actttgaagc ggtcgctcaa taccagtttg actttggtct gcgtccatcg
      961 ctgggttatg tcttatcgaa agggaaagat attgagggca tcggtgatga agatctggtc
     1021 aattatatcg acgtcggtgc tacgtattat ttcaacaaaa atatgtcagc gtttgttgat
     1081 tataaaatca accaactgga tagcgataat aagctgaata ttaataatga tgatactgtc
     1141 gcggttggga tgacgtatca gttttaatga atattgccgg atgtgatgca tccggcacat
     1201 ttcactcacg ccgttaactt caccggttca tcacgaaaat cattcgccgg ttccagcttc
     1261 agattcagcg tcgtcagcag ttcacgcagc ttctcgtgaa actcacgcag ggtgtgctcc
     1321 agtcgttcaa ccacttcagt atcttttacc ggaacactct tccagtcgcc tgctttatcg
     1381 aacagaccaa actggtagct gtaggtaaaa cgggattcct gcgcttccag ctccatccac
     1441 cagccccaga attcacgcac ttccggtgcc ggtttcacgt tgacgcatac agccagacaa
     1501 tcgaaaaaga atcgattatc ttcgcactta ccttcacgaa tatacgggcc tagtgcggta
     1561 aattttttga tcaatctgct cttcgggtgt ccactcggta acgtcattgc tatctcctgt
     1621 tgtgatgcaa ctgttttacc aaattggcaa aatttagcaa atttttaaca caagcgtttt
     1681 tcgatccagc cggtgatttc ctgaagacct ttgtcaaaat tccgatacac cgggttaaat
     1741 gggatctcta ataatttacc gtcagcagat gatgaggtga ttaagcgtga gtcctcttcc
     1801 gggctgaacg gatcgttctt ccagtagcct gataacattg gcgttgggca gcgacgcccc
     1861 agcaatcctt gcacttttaa tgaatagcga ttcagctcca cgcgtaacgc ttcgtcggaa
     1921 gcatcatgca tccccaaacg actcgccaga acgtcaagat acatttccgg cacctgttgc
     1981 tggcacttaa aatcactcaa cagggtatga actaccggac caagacaggc aaccgctttc
     2041 agacgcggcg attcaaggta tgccagacgc acggcaacgt tagcgccgaa acggaaacca
     2101 aaggccgcga cgcgagtgtg atccacccac ggtacgttag gcagcgcctt taagacgtgc
     2161 tgatgcaaca ggctggagtc ctgggtgagc ttccattttg aagaaaagcc caccgacggc
     2221 atatcaatag tcagcatcgc aatgccgcgc ggcgcaaaat aacgttcata caggctgtaa
     2281 tagtccgtct gcatcgcatc cagaccacca cacattaata ctgtcgggaa cgggccatcg
     2341 cctttcggca tatgcaaaaa gccggtgatg ggcgcaccgc cgggtacggt aaactccatc
     2401 tgccgcatcg tgcccggcag acgctgggcg gcctcttcat aggcgcggtt tgataaagcc
     2461 tgcgcttgct cggccaggtc atctcctttc agatgaggat aggcggcaat gttgtacaac
     2521 gtagccgcat gcagccagtg tcgaccactg agttgcggat cttcttccgt acaggctttt
     2581 tgttgccaca ccatcgcctg ggtggcccac tcataaatcc agttgccacc acggtagcca
     2641 atcacggtat cgtataaatc atcgtcggta cgttcagcat cgctcatcac aatacgtgcc
     2701 tggacgtcga ggatttcacg tgggtcaata ccgcgccaga tccacatcag acggttaatc
     2761 atgcgatacc agtgagggat ggttttacca tcaagagccg actgcacagg cggttgtgcg
     2821 ccgtgattaa agcggcggac tagcgtcgag gtttcaggat gtttaaagcg gggtttgaac
     2881 agggtttcgc tcaggtttgc ctgtgtcatg gatgcagcct ccagaatact tactggaaac
     2941 tattgtaacc tgcctgaagt taaaaagaac aacgcccggc aatgccaggc gttgaaaaga
     3001 ttagcgaccg gagattggcg ggacgaatac gacgcccata tcccacggct gttcgatcca
     3061 ggtatcttgc gggatatcaa caacatagtc atcaaccagc ggacgaccag ccggttttgc
     3121 gaagatggtg acaaagtgcg cttttggata catttcacga atcgcaacag cagtaccacc
     3181 ggtatccacc aggtcatcaa taacgatgaa gccttcgcca tcgccttctg cgcgtttcag
     3241 cactttaagc tcgcgctggt tgtcgtgatc gtagctggaa atacaaacgg tatcgacatg
     3301 acgaataccc agttcacgcg ccagtaacgc ccccggtacc agaccgccac ggcttacggc
     3361 aataatgcct ttccattgtt cagaaggcat cagtcggctt gcgagtttac gtgcatggat
     3421 ctgcaacatg tcccaggtga cgatgtattt ttcgctcatg tgaagtgtcc cagcctgttt
     3481 atctacggct taaaaagtgt tcgaggggaa aataggttgc gcgagattat agagatctgg
     3541 cgcactaaaa accagtattt cacatgagtc cgcgtctttt tacgcactgc ctctccctga
     3601 cgcgggataa agtggtattc tcaatacata tctcgcaagc ctgtcttgtg ttgacaacat
     3661 tttctgctaa ccctgtgacc tgcaatactg ttttgcgggt gatcgacaag gagacttaac
     3721 gtgtctgaac tgtctcaatt atctccacag ccgctgtggg atatttttgc caaaatctgt
     3781 tctattcctc acccgtccta tcatgaagag caactcgctg aatacattgt tggttgggca
     3841 aaagagaaag gtttccatgt cgaacgcgat caggtaggta atatcctgat tcgtcaacct
     3901 gctaccgcag gtatggaaaa tcgtaaaccg gtcgtcttac aggcccacct cgatatggtg
     3961 ccgcagaaaa ataacgacac cgtgcatgac ttcacgaaag atcctatcca gccttatatt
     4021 gatggcgaat gggttaaagc gcgcggcacc acgctgggtg cggataacgg cattggtatg
     4081 gcctctgcac tggcggttct ggctgacgaa aacgtggttc acggcccgct ggaagtgctg
     4141 ctgaccatga ccgaagaagc cggtatggac ggtgcgttcg gcttacagag caactggttg
     4201 caggctgata ttctgattaa caccgactcc gaagaagaag gtgaaatcta catgggttgt
     4261 gcggggggta tcgacttcac ctccaacctg catttagatc gtgaagcggt tccagctggt
     4321 tttgaaacct tcaagttaac cttaaaaggt ctgaaaggcg gtcactccgg cggtgaaatc
     4381 cacgttggcc tgggtaatgc caacaaactg ctggtgcgct tcctggcggg tcatgcagaa
     4441 gaactggacc tgcgccttat cgatttcaac ggcggcacac tgcgtaacgc catcccgcgt
     4501 gaagcctttg cgaccattgc tgtcgcagct gataaagtcg acgcactgaa atctctggtg
     4561 aatacctatc aggagatcct gaaaaacgag ctggcagaga aagagaaaaa tctggccttg
     4621 ttgctggact ctgtagcgaa cgataaagct gccctgattg cgaaatctcg cgataccttt
     4681 attcgtctgc tgaacgccac cccgaacggt gtgatccgca attccgacgt ggcaaaaggt
     4741 gtggtcgaaa cctccctgaa cgtcggtgtg gtgaccatga ctgacaataa cgtagaaatt
     4801 cactgcctga tccgttcact aatcgacagc ggtaaagact acgtggtgag catgctggat
     4861 tcgctgggta aactggctgg cgcgaaaacc gaagcgaaag gcgcatatcc tggctggcag
     4921 ccggacgcta attctccggt gatgcatctg gtacgtgaaa cctatcagcg cctgttcaac
     4981 aagacgccga acatccagat tatccacgcg ggcctggaat gtggtctgtt caaaaaaccg
     5041 tatccggaaa tggacatggt ttctatcggg ccaactatca ccggtccaca ctctccggat
     5101 gagcaagttc acatcgaaag cgtaggtcat tactggacac tgctgactga actgctgaaa
     5161 gaaattccgg cgaagtaatt atttgatttg ctgccggatg gcgtttaatc gccttccggc
     5221 agtttcatcc ttcattatcc ttcgataaaa gccatccctg taaacgttcg tcgcgggttg
     5281 ccacgttcaa tctggtgatg gaacattcgc cgctgtgatt tcagcaccgc gctattttcc
     5341 tattgctgct gatccagctt ccaggcaatc agcaatcgcg ccagccgttt gttagcatgc
     5401 tggctgcgct ctgactgaac cttcacgcta ataccggatg ccaaatgcgt ggcgcgtacc
     5461 gccgagtcgg ttttattgac atgttgaccg cccggccccg acgaacgcag cgtctcatac
     5521 cggatcgcat cggattgctc ctgctcatca gcggtaaaac gcccaatgcc cagaaaccag
     5581 tttttacgcc cgtgatgtgg gcgatacggg ctcgggcaaa tccactgaat agtgccacac
     5641 cagctttcgc ttaacgccca tgcgttgtcg ccatccagag aaatcagcgc cgaacgcagc
     5701 gtgtcagagt agcggcctgt ttctgtttcc agcaccgtta ccgcgacgtc ttgtcgggca
     5761 gcttctttaa ttagcctgtc cagcgccttt ttcactgcga gacaacattc ttccggcccc
     5821 tgcgctgagg agagttatag caagatcatc cttttttccc tccactgttt ttgagcgtta
     5881 gcaccggacg cagccgcgcg acaggaataa ttaatccggc ctgccctgac attgcaccac
     5941 actttcagcc gatttatagg cttgtggcgc ttcttcgaag atgagttgtt tatcgcgaca
     6001 aattacccga ctgccaagtt cagtacgcga gagttgcgtc gctgtgtatt tcgctgccag
     6061 acgcccttta cactcggtgc gcccccattt acgcccagcc ccatgcgcca gcgaatgcaa
     6121 tgtttcctca ctcctgacgg gctgaaccag cctggagtaa tcaccgcgtg aaccgggaat
     6181 aaccaccaga ccacagtcat caggcgtggc acctttacga tgtaagaccc cttgttgatc
     6241 accgattcga cacgcgctaa caaagttatg cgccacatcc agaaccggac ttccggtggc
     6301 cttaacctgt tgcattatgc gcaaagcgat caggtggcga ttaaaacgcg caaacgccag
     6361 cgcgtcgtca ctgccttcag gcaaaccatg atgcgaaaac gaggcaatat gccgctgtaa
     6421 aatagactgc cctagccccc tcgagccgct atgaaccagc agttgcagat gctgcgcatc
     6481 cagacctgcc agtgcaaaca gttcagcgtt gatgatttga tcaacttgtt gcagttcagc
     6541 gaaatgattg ccaccaccga tagagccaag cgagctgcgc cacggatgct gtgcaaatgc
     6601 tgacggcagg ttttcctcca gccagctttc ttcagcaacg tcatccaaag ctgataatcg
     6661 cttttcaaac ttatcggcgt tgtatttgcg agcgagaata tctgtttgcc atagcgccat
     6721 gccacagccg atatcattgc cgaccagtgc tgggtaaaaa cggcctgcgg agaagaacgc
     6781 tgcgccaatc gggtagccgc gtccggggtg taaatctggc atccccacta cgcgctgcat
     6841 gttgggtaaa tttgccgtgg tgtgtaattg ttggatcgct aaactctcga tccacaggtc
     6901 atcagatgcg atggtaaata ccgcatcgga taagggacga atataattgc ccatatacca
     6961 ttccgtaaat agactcgtca tacttcaagt tgcatgtgct gcgacttgaa ttattgagag
     7021 taacaattaa aaaatcagga aatggcagac gaatccaggg caagaaaaaa agaattaatc
     7081 ctggaaaggt ctgcaaagag tcattgataa aggatttgtc atgatttacc tccttgcagt
     7141 ttgtggaata agaaaacgcg cacatactag gcgggcgtta ttttcattgc aagctggatt
     7201 taatgttgcg gtttataacg catataaaaa ttaataaacc acgctccccg gcattcaacg
     7261 cgctgctgct taactgtctg aaaaccataa cgttcaaaaa agggttttgc ggttatgctt
     7321 gcgtccaccg taagttcgga ttcagactta ataaaaggtt ttaacaaagc gctggcaacc
     7381 ccacggcggg tgtattcagg gtcaacaaat aacatatcga tatagtgttc aacgcaggta
     7441 ataaagccga ccagttttgc attaataact gcaacccgca cttgtgattt cgcgagtttc
     7501 tccttccagc gagattcgtc aatctgcgcc caggcggcaa tttgttgtag tgaataatgc
     7561 tgactgacgg tcatcgtaac cgctctaatg aaaatagcgc atagttgctg aaaatcgcca
     7621 ggctgataat ctcttatttg tatgttattc ataatataaa ttcaaaaacg catgcgaaac
     7681 gcttctgcca tttttccaat tttatgcatt tggttgttat ctcgagccag tttatgaggt
     7741 tcaggcttca gaatggcaat gagcaaccat gcttgctcat caaacgcacc ctgacaatat
     7801 accaaatgcg cttcgtcatt cgttctgctg aattggcgca actgtggcgg aaatggatta
     7861 ttcacatttg ccagatgaat atgagcaact cgctcaaatt tgattaatgg ccaggtaaac
     7921 gagtcgtcgt agagtgcatc gcgaccaaat atatctggca aaacaccgtc acgcttatag
     7981 gaaataaaat ccgccgttaa cgcatcaagt tcctctgctg taagttgcag gcgaataagt
     8041 tttgttttga atacccgcat ccttattcct taaagtcatt gaaatcatca tccgtcatat
     8101 cattaagata ttgtttagcg cggcgtgtaa tttcctctaa tcttgcagca cttggacgaa
     8161 agcgcgcctt tatgggcttt ttctcctctt gttcagcaag catgtccatt aacgcttcga
     8221 atgcgctggc acttaagaga tatcctgcgg ggcgattatt agaaagaacc gcaactggtt
     8281 gatcaataaa gtatttagct gggtttttac gtaactcagt gatattgacc gatttttcag
     8341 cgagaattcg atgcatacag tgataccctc ataataatgc acaccagaat atacataata
     8401 gtatacatca taatcccagc accagttgtc tttccatttg cgggtcaagc aacgtcacat
     8461 gcagccccac cagacgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     8521 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgggtg gtctgctgaa
     8581 aatcgtcgaa ctttaatttc accccctggc gagcaatcag taaatcaggt tttacctttg
     8641 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     8701 gatgaatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgtaac cgttcgctgt
     8761 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     8821 agcgtttaag cagaatcacc agatcacact tttgtacatc accgcaggtc cgcagcccca
     8881 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gattttttcc agtggtaagg
     8941 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctgaccgttg ggtttattca
     9001 tgtcggaggc gattttggcg agaaacttta ctggtgccac gcccgcagat gccgtcagtt
     9061 gcagctcgtt gaagatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     9121 aatggacgct atcggtgaca tcgagataag cctcatccag tgacaacggt tcaatgcgcg
     9181 aggtgtagcg cgagaagatt tcacggatat gatttgaggc ttctttgtag gcgtcaaagc
     9241 gccccggaag caaggtgaga tgtgggcata atttgagcgc catcccagta ggcatagcgc
     9301 tacgtacgcc aaatttacgc gcgggataat tggcggtgct gatcaccccc cgacattcgc
     9361 ggctgccgcc aatagcaata gggatatcgc gcagagcggg attgtcgcgc atctccaccg
     9421 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     9481 tggtaaagta tacagtgatt tcaggttttg agaaatgcgt aaagattcag catttagcgc
     9541 attcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     9601 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     9661 atctcaatac gccgattgcc cgcgctttgc ggattcttcg cgtccagcag catctggtcc
     9721 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     9781 cgagctgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtag
     9841 gccatcgcat cggtatgccc ggtaataata attttgttat agagcgagtc gaacactggc
     9901 gcgagctcca ccagcaaagt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     9961 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
    10021 tccagattcg cttccatatg cgcatcacgg gcaatggtgt tgatactggt tgccaactcg
    10081 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattcac atcacgcgct
    10141 tttttctccg tctcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
    10201 attttattta acggcgacag cccaccaccg ttaaaaatcg actgcccgtg cagcgcggcg
    10261 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat cgtcgtcacc
    10321 aaagaggccg agcgtatttc cgaggtttct gcccgcttta ccctggacgc gatgcccggc
    10381 aaacagatgg cgattgacgc cgatcttaac gccggattga tcaaccaggc gcaggcgcaa
    10441 acccggcgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
    10501 aagtttgtac gcggagacgc catcgccggg atgatgattc tggcgatcaa cctgagcggc
    10561 ggcgtctgta tcggcatctt caaatacaac ctgagcgccg acgccgcctt ccagcaatac
    10621 gtgctgatga ccatcggcga cggcctggtg gcgcagatcc cttccctgct gctctccacc
    10681 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgc
    10741 caccagttgc tggcaagccc gtcggtgctc tacaccgcca ccgggatcat gttcgtgctg
    10801 gcggtggtgc cggggatgcc gcatctgccg tttttgctgt tcagcgccct gcttggcttt
    10861 accggctggc ggatgagcaa acgcccgcag gcggcggagg cggaagagaa aagcctcgaa
    10921 acgctgaccc gcaccatcac tgaaaccagc gaacaacagg tcagttggga aactattccg
    10981 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcg
    11041 cagggcaacc cgctcaccca gcggattcgc ggcgttcggc aggtgatttc cgacggtaac
    11101 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gcctcaagcc cagccagtac
    11161 gctattttca tcaacggcat tgaggctgat gaagcggata ttccggcgga taaactgatg
    11221 gcgctgccct ccagcgaaac ctacggcgag attgacggcg tgctggggaa cgacccggcg
    11281 tacgggatac cggtcacctg gatccagcct gcgcagaaag cgaaggctct gaatatgggg
    11341 tatcaggtga tcgacagcgc cagtgtgatt gccacccatg tgaacaagat tgtgcgcagc
    11401 tatattcctg atttgtttaa ctatgatgac atcacgcagt tgcataaccg tttgtcgtcg
    11461 atggcaccgc gcctggcgga ggatttaagc gcggcgctca attacagcca attgctgaaa
    11521 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
    11581 gtgctggtcg ccagtagcac ggtgactaaa gatcatattc tgctggcggc cgatgtgcgc
    11641 ctggcgctgc ggcgcagcat tactcatccg ttcgttcgca agcaggagct gacggtgtat
    11701 acgctgaata atgagctgga aaatctgctg accaacgtag tgaatcaggc gcaacaggcc
    11761 ggcaaagtga tgctcgacag cgtgccggtt gacccgaata tgctcaacca gttccagagc
    11821 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagatccggt gctgctggtg
    11881 ccgccgcagc tacgcccttt gctgccgcgt tatgcaaggt tgtttgcgcc ggggctgcat
    11941 gtgctgtcgt ataacgaagt gccggatgag ctggagttga agattatggg ggcgttgatg
    12001 taagaatccg gcatcggtgc acaattgccg gatgcggcgt aaacgcctta tccggcctac
    12061 cgttcaggca caagttttta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
    12121 cataactgcc ggatgcggcg tgaacgcctt atccggccta ccgttaaagc acgatttgta
    12181 ggccggataa gcgtaagcgc atccggcacc agcgcacagt tataaaatga tacgctcccc
    12241 ggcacttaaa tccgttatgt ctcccgccca atcgatggga tataaccctc gcgcgacatc
    12301 gcgatggaac gttgagaatg gccaatcact cacctgcttt acccagccat gtttctctgg
    12361 atttatataa atataatcaa catgatggcg ataatctttt gtattgcgaa tggcatgctc
    12421 ccaaaaacgc ggttgccaga tatttttcaa tccgcaagca tgggtgaact gctttttaat
    12481 ctcccgccag cgagaggaaa aatcatcatc gctttcgggt aatgtccata tgcaatgcat
    12541 atgctccggc aaaacgaccc aggcgttaat ttcaaaaggt ctgtctcgct taacgttaat
    12601 aatggcgtta cggagcgtct ggaactgggt ggtcagaaga tggcttcgac gattgcgtaa
    12661 atttaccgtg aaaaaccatg ttcccccctt gatgtaataa cgacgatatt ccgacatagc
    12721 gactcctttt tctcaacggt aagaggaaaa ggaaaggccg acaatgacgc tgtgttatat
    12781 cgggaaagcg gctcgcaaaa acgcacagat ttgtaggcat gataagacgc gtcagcgtcg
    12841 catcaggcat ccgtgcacaa ccgccggatg cggcgtaaac gccttatccg gcctacaaaa
    12901 tcatctcttc cgtcaaaatc ctgcgcgcgc ctaaaaaatg gtcctgccag aaaggttcgg
    12961 ctaaccggct tacccgaatg gtttccccgg tgcgcagtga ctcaataaac tgaccgtcgc
    13021 ccagatacac ccccatatgg tcggcaatct cgcggctgtg gatatggaaa aatagcaaat
    13081 ctccccggcg caggtcgtta ttcgccacaa tcgttgcccg acggtagtgg tacatctcat
    13141 tggccgtgcg cgggagtttc gcctcaagga tcttgttgta ggcataaaaa actaacccac
    13201 tacaatcgaa gccttgatcg ggccgcgtgc cgccccagac gtaaggcttg ccaagctgct
    13261 gctccaggcg gtgaatcgcc acttcggtga tgttatgcag gtgatcgctg cttaaaaagt
    13321 ggttgttttc cgccagcgcc tgccagcgtc tgtcactcgc cttgagcggc gcaggaaacc
    13381 attctggatg ctgttttatc tgctcccggt tgtggtggcg ctggcgagta aagggagcaa
    13441 aacgcataac gaaaacacgc ctggatgcaa aaatctgccg agcaagaacg aagacatgaa
    13501 agacataaac cggctgaatg aaggtgagaa ttttaaaaga ccggaggagt aaacggga
//
LOCUS       DAZPAB010000100        13242 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZPAB010000100 DAZPAB010000000
VERSION     DAZPAB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.100"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..228)
                     /locus_tag="ACHUPG_002573"
     CDS             complement(<1..228)
                     /locus_tag="ACHUPG_002573"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862136.1"
                     /translation="MPEQVITKAPYIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHSKPLEIIKTQ"
     gene            165..1664
                     /gene="pitB"
                     /locus_tag="ACHUPG_002574"
     CDS             165..1664
                     /gene="pitB"
                     /locus_tag="ACHUPG_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR0862137.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1713..2405)
                     /locus_tag="ACHUPG_002575"
     CDS             complement(1713..2405)
                     /locus_tag="ACHUPG_002575"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR0862138.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2579..3292
                     /locus_tag="ACHUPG_002576"
     CDS             2579..3292
                     /locus_tag="ACHUPG_002576"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR0862139.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            3324..4082
                     /locus_tag="ACHUPG_002577"
     CDS             3324..4082
                     /locus_tag="ACHUPG_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862140.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            4128..5477
                     /locus_tag="ACHUPG_002578"
     CDS             4128..5477
                     /locus_tag="ACHUPG_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR0862141.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5477..6301
                     /locus_tag="ACHUPG_002579"
     CDS             5477..6301
                     /locus_tag="ACHUPG_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862142.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            6313..6873
                     /locus_tag="ACHUPG_002580"
     CDS             6313..6873
                     /locus_tag="ACHUPG_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR0862143.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6904..7974
                     /gene="lptF"
                     /locus_tag="ACHUPG_002581"
     CDS             6904..7974
                     /gene="lptF"
                     /locus_tag="ACHUPG_002581"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR0862144.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            7971..9050
                     /gene="lptG"
                     /locus_tag="ACHUPG_002582"
     CDS             7971..9050
                     /gene="lptG"
                     /locus_tag="ACHUPG_002582"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR0862145.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9086..10257)
                     /locus_tag="ACHUPG_002583"
                     /pseudo
     CDS             complement(9086..10257)
                     /locus_tag="ACHUPG_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(10257..10505)
                     /locus_tag="ACHUPG_002584"
     CDS             complement(10257..10505)
                     /locus_tag="ACHUPG_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR0862146.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10538..11452)
                     /locus_tag="ACHUPG_002585"
     CDS             complement(10538..11452)
                     /locus_tag="ACHUPG_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR0862147.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11449..13137)
                     /locus_tag="ACHUPG_002586"
     CDS             complement(11449..13137)
                     /locus_tag="ACHUPG_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFR0862148.1"
                     /translation="MRYADFPTLVDALDYAALSRAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPSDRCVSWQPFYHDMG
                     LVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
BASE COUNT         3182 a         3241 c         3439 g         3380 t
ORIGIN      
        1 ctgtgttttg attatttcta aaggttttga atgaatgctt attgtctgat acacaagaaa
       61 taacactctt tttatcgtta aaaaatgata tttcactttg cccatgccgt taaagtcact
      121 gataatgcgt ccgttcgtaa attcaaaatg gcgtaatcta atatatgcta aatttatttg
      181 ttggccttga tatatatacg gggcttttgt tattacttgc tctggcattt gtgttgttct
      241 acgaagcaat caatggtttt catgacacgg cgaatgcggt ggcaaccgtt atttatactc
      301 gtgccatgca gccacaactt gctgtggtga tggcggcatt ttttaacttt tttggcgtgt
      361 tattgggcgg acttagcgtt gcctatgcca ttgtccatat gttaccaatc gatttgttgc
      421 tgaatatggg gtcaactcac ggtttggcga tggtcttttc catgctgctg gcggcgatta
      481 tctggaacct gggaacgtgg ttctttggtt taccggcctc cagttcgcac accttgattg
      541 gtgcgattat cggcatcggt ttaaccaacg cgctgttaac cggctcatcg gtgatggatg
      601 cgttaaacct gcgtgaagta accaaaattt tctcctcgct gattgtttcc cctatcgtcg
      661 gcctggtcat tgcgggtggt ctgattttcc tgctgcgccg tttctggagt gggacgaaaa
      721 agcgtgaccg tattcaccgc attccggaag atcgcaaaaa gaaaaagggc aaacgtaaac
      781 cgccattctg gacgcgtatt gcgctgattg tttccgctgc gggcgtggcg ttttcgcacg
      841 gcgcgaacga cgggcagaaa gggatcggcc tggtaatgct ggtactggtg gggattgccc
      901 ctgctggctt cgtcgtcaat atgaatgcgt ccggctatga aattacccgt acccgcgatg
      961 ccgttaccaa cttcgaacac tacctgcaac agcatcctga actgccgcag aagttgattg
     1021 cgatggaacc tccattgcct gcagcatcga ctgatggcac gcaagtaaca gagtttcact
     1081 gtcatccggc aaataccttt gatgcgattg cgcgcgttaa aacgatgctg ccaggcaata
     1141 tggaaagtta cgagccgtta agcgtgagtc agcgcagcca gctgcgccgc attatgctgt
     1201 gcatctccga tacctccgcg aagctggcga aactgccagg cgtcagtaaa gaagaccaga
     1261 acctgctgaa aaaactgcgc agcgatatgt taagcaccat tgagtacgct ccggtgtgga
     1321 tcatcatggc ggtagcactg gcgctcggca ttggcaccat gattggctgg cgtcgtgtag
     1381 cgatgaccat cggtgagaag attggtaagc gcggcatgac gtatgcgcaa ggcatggcgg
     1441 cacaaatgac ggcggcagtg tctatcggcc ttgccagtta tattgggatg cctgtctcca
     1501 caacacacgt cctctcgtct gcggtagcag ggacgatggt ggtggacggc ggtgggttac
     1561 agcgtaaaac ggtaaccagc atcctgatgg cgtgggtgtt tactttaccg gcggcaattt
     1621 ttctttctgg tgggctgtac tggatagcat tgcagttgat ttaaccttcc tgaaaatgcc
     1681 cggtcctggc gatcgggcat ttccattttt gactagtgat aaccacgcgc ggtcataaaa
     1741 tccgtaatcg ctttttctgc atcaaccaac acctgttcca gtggctgatt ggcatcgata
     1801 tcaaccagtt gtgcaccacc aaaggttaac tgtggcgtta tggcaatctt cctcgccagc
     1861 gattcccgtt tatggtcggg tttacgtgca caggcaactt caaggtcaac attgagtttg
     1921 atgaccagat caggcttatg gctcgccatc cagtgaaacg ctttacgttc ctggcttgcc
     1981 agccatgaga caaaacgacc accttcaacg ttaggtggga acaccgtacc atcgtaagcg
     2041 ccaggaattt ggtcctgagg ataacggtcg gttagaacaa ttaacccgcg acgacgacag
     2101 gcaagcatat gacgaaagcg cagtaagcga cgggcgacaa acgctgtaat taccagcgcc
     2161 ggaactggtc caggcaattt ttttgctgtt ttcacctgat ttcgttcaat tgttttatgt
     2221 aaggattttc ccatcaaagg taattttgtc actgcacgac cgacatttcc ggcctgtttt
     2281 cctaaatgaa ctctttcggc agcaccatat ttttcgacaa cggtaataag atgttcacac
     2341 accgttgact tgcctgaagc atcgctacca ataacggcaa ttaatggagg tgttattgac
     2401 tgcatgaata tatcctttaa tttaaatatc cattaaaaat atttatttgg ttaatatgtt
     2461 tttatgaaag cgtaattcag gtcaatgtca caattagtca ttgtcacaat aaggttgaac
     2521 ggatattctg agcacggacc acgtaatatt caatacatta tttactgccg ggtttttcgt
     2581 gaatcaacgt aatatgtcaa taattaattc cacgccagtg cgtgttattg ccattgtagg
     2641 atgtgatggt tcaggtaaat cgaccctcac ggcaagcctg gtaaatgaac tggcagcaag
     2701 aatgccaaca gaacacattt atctcgggca atcgtccggg cgaattggcg aatggatttc
     2761 acagctccct gttattggcg caccttgtgg gtgttatctg cgaagtaaag cggcacatgt
     2821 acacgaaaag cccttaactc cgcctggcaa tattactgcg ctggttatct atctgctttc
     2881 ctgctggcgg gcgtacaagt ttcgcaaaat gttgtgtaaa agccagcaag gctatctgct
     2941 catcaccgac cgctacccgc aggttgaagt gccgggtttt cgctttgatg gcccgcaatt
     3001 ggcaaaaacc acgggcggta acggttggat aaaaatgtta aggcagcgcg agttgaagct
     3061 gtaccaatgg atggcgtctt atttacccgt attgttgatt cgtcttggca ttgatgaaca
     3121 aaccgcgttt gcgcgtaaac ctgaccacca actggcagcg ttacaggaaa aaatcgccgt
     3181 tacgccgcaa ctgacattca atggcgccaa gatccttgaa ctggatggtc ggcaaccagc
     3241 cgatgaaatt atgcaagcgt cactacgcgc aattcacgcc gcgctttcct gatcaccaaa
     3301 atggataggg gatgatttaa cgaatggatg cactacaaac acaaactgtt aatagtacaa
     3361 ccgcgccgca gcccaactac attccggggc tgattgcggt ggtcgggtgt gatggcaccg
     3421 gtaaatccac gctgaccacc gacctggtga aatcgctgca acaacactgg caaaccgagc
     3481 gccgctatct ggggctgctc tccggcgaag acggcgacaa aatcaaacga ttgccgttgg
     3541 ttggcgtctg gctggaacgg cgattggcgg ccaaatcctc gaaaacccaa agcatgaaaa
     3601 ccaaatctcc ggcgctatgg gcggcggtga ttatgtactg cttctcgctg cgaagaatgg
     3661 cgaatctacg caaggttcag cgactggcgc aaagtggcgt tctggtggtc agcgatcgct
     3721 tcccgcaggc tgaaatttcg ggcttttatt atgacggacc ggggatcggc gtcgaacgtg
     3781 cgaccgggaa aatcagcatg tttctggcgc agcgcgaacg gagtttatac caaaaaatgg
     3841 cgcaataccg tcctgaatta atcattcgtt tggggattga tattgatact gccatctccc
     3901 gcaagcctga ccatgagtat gccgagctgc aggacaaaat cggcgtcatg tcgacgattg
     3961 gctataacgg cacaaaaatt cttgagattg attccagagc gccctacagc gaagtgctgg
     4021 aacaggtaca gaaggcggtt tctctggttg ccatcgtttc tgaccgccga agtttaactt
     4081 aaggctggcg ggatattttt tactatcaac gataaggtca ggctgaattg gcgggtttta
     4141 acatcaaaca ttggtttgca gatggcgcgt ttcgcaccat tattcgcaat agcgcctggt
     4201 taggctccag taatgtcgag agcgccttgc tggggctgtt ggcgctctcg tgtgccggta
     4261 aagggatgac gcccgccatg tttggtgtac tggtgattgt gcaatcgtac gccaagtcga
     4321 tcagcgattt tattaagttt cagacatggc agctggtggt tcagtacgga acgccagcct
     4381 tgaccaacaa taatccgcag caatttcgca atgtcgtctc attttccttc tcgctggata
     4441 tcgtcagcgg cgcggtggcg attgtcggcg gcattgcctt actgccattc ctttcccatt
     4501 cattaggtct ggatgaccaa agcttttggc tggcggcgct gtattgcacg ctcattcctt
     4561 caatggcttc ctccacgccg accggcattc tgcgtgcggt agatcgcttt gatttgattg
     4621 ctgtacagca ggcgacgaaa ccttttctgc gcgcagcggg gagcgtcgca gcctggtatt
     4681 ttgactttgg ttttgtgggt tttgttattg cctggtacgt gtcgaatctg gttggcggca
     4741 ccatgtactg gtggtttgcc gcgcgcgaat tacgccgtag aaatatccat aacgccttca
     4801 aattgaatct gtttgagtct gcccgacaca ttaaaggcgc gtggagtttt gtctggtcaa
     4861 ccaacattgc ccactccatc tggtcggcgc gtaactcgtg cagcacagtg ttggtgggga
     4921 tcgtgttagg acccgctgcc gccgggttat ttaaaatcgc catgacattc ttcgacgccg
     4981 ccggaacgcc agcgggtttg ctgggtaaaa gtttttaccc agaggtgatg cgtttagatc
     5041 cgcgcaccac cagaccgtgg ttgttaggtg tgaagtccgg tttactggcg ggcggaatcg
     5101 gtattctggt ggcgctcgcg gtgttgatcg tcggcaagcc gctcatttcg ctggtgtttg
     5161 gcgttaagta tctcgaagcg tatgacctga ttcaggtgat gctgggcgca attgtgattt
     5221 cgatgctggg cttcccgcag gagtcattgc tattgatggc gggaaaacag cgcgcttttc
     5281 tcgtggcgca aaccatcgcc tcaatcggct acatcgtatt gctgttcatg ttctgtcatc
     5341 tgtttggtgt gctgggagca gcgtttgctt acttcggtgg gcaatgtctg gacgtggcgc
     5401 tctcgttaat tccaacctta aaggcatttt ttcagcgcca ttccttgctt tataacgcag
     5461 ccggagagaa atcctgatga tgagaaaata tttcccgctt gaggcgagcg agcgtctatt
     5521 tgttgcgatt gaagaagatg atgtcgttga tgcacaggtt agtttgccgc cgactattgc
     5581 actaagttgt accaccgaaa tcattcatga taattacgcg ttatgcctgc aattctggct
     5641 caatggcgtt gatcgtcagg aattactgcg tttagttcgc aaacaagcca aaggcgatga
     5701 gctgactgct gatgagcgca aacaatttaa atacatgcga gcgcgctaca agcacctgcg
     5761 ttttgcgcaa cggctgtatt taaagaagca tcaggccgga tttttattcg gcaaaacgac
     5821 cgtttttctg gggcgttttc aggacggctt tcgtaacggt aagaaaaata ttgtgtcgta
     5881 ttacggcaac ctgttacgga tttatttaag ctctccggtc tggtcgttgg tgaactattc
     5941 attacgccat agccagttag aaagcgtaag cagttttatt gcctatcggc aaaagcaaat
     6001 gcataccctg aaagagatta ttgccaaacc gcgactaacc ggcagggaat ttcatgatgt
     6061 gcgaaaaatc atcagccagc aggtttctta ttacgacacc ttaagatcgc ttgatcctga
     6121 aaataaagag gcgttacaga tttcccgttt cctcgccgcc attaatggcc tgatgggaga
     6181 taaacacgac gacatggtgg cggatgatat ggaaaaccgc cagtcgtatt atgcaccgtt
     6241 ggcgctggat agcgatattt gtcagcgctt agagctgctg atttcgcgtt ttccgctata
     6301 gccggagcaa tgatgtcatc tcgtttgatt attgcgttga ttatcatgct cttagccccg
     6361 ggtgtgcagg cacacaattt cgtcactggc aaaacggtta cccctgttta tatccaggaa
     6421 ggcggcgagc tgcttctgaa tagcgacgac gaaattcact accaaaagtg gaacagcaca
     6481 caattagcgg gaaaagtccg cattattcag tatatcgccg ggcgtaagtc ggcgaagaaa
     6541 aagaactcac tgttaattaa agcggttgaa gcggcaaact ttccgcagga tcgttttcag
     6601 cccaccacca ttgtgaacac cgatgatgcc atctttggta ctggctattt tgttgttggc
     6661 aaaatcgaaa aaaataaacg ccgctatccg tgggcgcaat tcgttattga cggcaatgga
     6721 cagggacgcg ttgcctggcg cttgccggag cagagttcaa ccattctggt gctgaataaa
     6781 gccgggcaaa ttcagtgggc aaaggacggc agcctgacgc cggaagaggt agatcatgtc
     6841 attgcgttgg cgcagaaact gatcaatgaa taaaaaactc aaaggattga gggcgttagt
     6901 gagatgaagc tggttgaaca ctacatcatg cgtggtacgc gccgtctggt gctgattatc
     6961 gtcggttttc tgatctttat tttcgccagc tactccgcac agcgttatct gaccgaagcg
     7021 gcaaatggga cattagctct tgatgttgtg ctggatatcg ttttctacaa agtactgatt
     7081 gcactggaga tgttgttacc tgttggtctg tatgtgtcag ttggcgtgac gctagggcag
     7141 atgtataccg actcggaaat taccgctatc tctgcggcgg ggggcagtcc gggacgcttg
     7201 tacaaagccg ttctttatct ggcgataccg ctaagtattt ttgtcaccct tctgtcgatg
     7261 tatggtcgtc cgtgggctta tgcgcagatt tatcaactgg agcagcagtc gcagtcggag
     7321 ctggatgttc gccagttgcg ggcaaagaaa tttaacacta acgataacgg acgaatgatc
     7381 ctttcgcaga cggttgatca ggataataat cgcctgactg acgcgctgat ttatacttct
     7441 actgccaatc gaacccgtat tttccgcgcc ggttcggttg atgtggttga cccatcaccc
     7501 gagaaaccga ccgttatgtt gcacaacggc actgcctatc ttctcgatca tcaggggcgt
     7561 gacgacaacg aacagatcta ccgtaatctg caattacatc tgaatccgct ggatcaaagc
     7621 ccgaacgtca aacgcaaagc gaaatcggtc acggagctgg cgcgctccgc ctttcctgcc
     7681 gatcatgccg aactgcaatg gcgacaaagc cgtggcctga ccgcattgtt gatggcgctg
     7741 ctggccattt cattaagtcg ggtaaaaccg cggcaagggc gattttcaac gttattgcca
     7801 ctgacgttgc tgtttattgc cattttttat ggcggcgacg tctgccgtac actggtggct
     7861 aacggcgcga ttcccctcat tcctggtttg tggttagtgc ccggactcat gctaatgggc
     7921 ctgctgatac tggtcgcacg tgacttctct ttgctgcaga aattttcccg atgaatgttt
     7981 tcagtcgcta tttaatccgt catctctttc tcggttttgc cgccgctgca gggctattgc
     8041 tgccgctttt taccaccttc aacctgatta acgaactgga tggtgtcagc ccgggcggtt
     8101 atcgctggac tcaggcggtg ctggtggtgc taatgacctt accgcgcaca ctggtcgaac
     8161 tttcgccgtt tatcgcgtta ttgggtggta ttgttggact ggggcagtta tcgaaaaaca
     8221 gtgagcttac cgccattcgc agcacggggt tttctatctt ccgtattgca ctggtggcgc
     8281 tggttgccgg gatattgtgg actgtttcgt taggcgcgat agatgagtgg gtggcgtcgc
     8341 cattgcagca acaggcgttg caaatcaaat cgaccgccac cgcgttgggg gaggacgatg
     8401 acattaccgg caatatgctg tgggccaggc gtggtaatga atttgtgacg gtgaaatcgc
     8461 tgaacgagca gggccagccg gtgggcgtgg agatatttca ttatcgcgac gatctctcgc
     8521 ttgaatccta catttatgca cgcagtgcca ccattgaaga cgacaaaacg tgggtcctgc
     8581 atggtgtgaa tcataaaaaa tggctgaatg gcaaagaaac gctggaaaca ttagataatc
     8641 ttgcctggca atcggccttc accagtatga atcttgaaga gttatcaatg ccggggaata
     8701 ctttttctgt ccgtcagctt aatcattaca tccattattt gcaggaaacc gggcaaccca
     8761 gcagcgaata ccaccttgca ctgtgggaaa aactggggca acccatcctg accctggcga
     8821 tgattttgct ggctgtgccg tttaccttta gtgccccgcg ttcgcctggg atgggtagcc
     8881 gtctcgctgt aggcgtcatc gtgggcttac tcacctggat cagctatcaa atcatggtta
     8941 atctgggatt gctatttgcg ttaagcgcac ctgttaccgc gctcggttta ccggtagcgt
     9001 ttgtgttggt ggcgttgagc ctggtgtatt ggtatgacag acaacattaa acccgccgtg
     9061 gcgcaaacac cacggcgggc tcacattact gcttcagagt agcaaatgcc tgaatgatgg
     9121 catcgatttg ctcacgactg tgggcagcat ttacgctaca gcgcagcagc gtgatgcctg
     9181 caggcgcggc aggcggcagg ataagattca catacacgcc cggcgagata agctcgcgcc
     9241 agatgcgtaa accttcctct ttcgaaccga ttattactgg cactaccggg ctaatatgtg
     9301 aacttaactc ataacccagt ttttgcagac cgtcatacag atggttggcg ttatccatca
     9361 gcttttgccg cagctctggt tgtgtggcaa tagttcttaa cgacgagcgc acggtcgcaa
     9421 tgcaggacgg agaaggagag gcggtaaaaa tatacgggcg gctgccgtag cgcagcactt
     9481 ccatcgcttc cgaaacgaca gcaaaaccgc caatcgaggc cagactttta ctgaaagtac
     9541 caacgataat atccacatca tcttcaacgc cgacagcttc agccagtccg cgccccgttg
     9601 cgcctaacac gccaaacgaa tgcgcttcat cgacaatcaa ataaccaccg agacgacgtt
     9661 tgatatcaac aatttccgcc agcggcgcaa cgtcaccgag catgctgtag atgccctcaa
     9721 cgatgataat cgcctctttc gcccgttcac cgaggcgaac catgcgccgt tccagatctt
     9781 tagcatcgtt atggcggaaa cgaataattt ccgcgccacc gagagaacag acgtcataaa
     9841 tactggcgtg gctgtcggca tccagtaaca ccacggcgtt atggtcggca agtgcgctga
     9901 tgacgcctaa attagcggta taaccggttg aaaaacgata gcggtagggc gattgaaaaa
     9961 ctcggcaatc tctttctcta acgccaggtg cggcgcataa ctgccgtttg ccatacgaga
    10021 accggtggtg ccggtgcctt gggccgctaa cgcggcctga ccttcgctga tggcatcatg
    10081 attaaaggtt aaaccgagat aattattggt cccggccaga atcacttttt tattgccgat
    10141 ccggccttcg gtggcggaat acacttcatc aatacaggtg ccaaacggtg ttaacccgtt
    10201 gtcactaaat tgcagacgct cgccagcaag gcgcgcatat ttatcgtata gccccattat
    10261 ttattctcca gccatggaag tagagtttcc attaattgcg caggggtttt gacatccagc
    10321 agaatattaa taggaatgga aatatcgaat ctgtcttcca gcatcattaa gagatccatc
    10381 actttgatcg actccaggcc aagatcgttg acgagatcgc tgtcgggctt aatttccaca
    10441 ccgttttcga ccaaatcctg taaacaggaa aggatgtaat ccattactat ttcacgattt
    10501 accatagaaa cgttaacaca cctttaaaac aagacaatca aaatagcccg ttgcgtaatg
    10561 cgtgttccag gctaatcccg ggaaaccaat tgatatcttc agaaatacgg ttattacttg
    10621 ccgaccagtc ggcgtgggtt aattcacgaa ttttcgagcg ggtcagcata ggttctttac
    10681 ctgccaggcg actcaacgcg gtactgatat ccgcaaggcc tgtgagtacc ggcagaggaa
    10741 tgtcaaccat tcgcacggaa ccacaacggg ctttggcggc aagctgtcgt acgcgttgcc
    10801 agtcatagcc accggcgacg ccatcgcata attcataggt ttgcgtctgt acagtttcgg
    10861 cgcttaacca ctgacccact gcttgcgcaa aatcggtgac gtgaagaaaa gagagttgtg
    10921 tatctggcgc accaagtcgt ggcagcaggc cgcgtagcat ccagtcaaac agcggtttta
    10981 actctttatc gccgggacca tacacggctg tcgggcgaaa aacgcccagc gtaatttcgt
    11041 ccgccattgc tgccagtcgt tgctcggcga cgtatttgga ttttgcatac caggagagct
    11101 cgggatggcg cgccgccagc gaagagataa acagaaaacg ttggcaaaag ccgctctctt
    11161 ttgctgcttg catcaggcgc aggctgccgt caacgttaca gcgggtgaaa atctcttctt
    11221 tgtgcccgcg cacttgcccg gcgcaatgga ccactacgct ggctccggca accagctcgc
    11281 taagtgaatg agtatcttcc agcgaaccgc gcacccaggt gagattatca ttgacgtgag
    11341 cgcgggcagt acgcgtcaat gcgcgaacgt gaaagccgcg ggcgagcagg ttatcgataa
    11401 tatatttacc gataaacccg gtagcgcccg tcaccgcgac agtttgattt atgccaggga
    11461 ttcctgcaca ttaagactgg cagcataagc cttctgataa cgttttttcg cttccgcacg
    11521 ggcaggcttg ccggaggacg ttcggggaat actgtggggc ggcaacagat cgatagccgc
    11581 ggtcacgcca aattcgcttt ggatccgtgc cgccagcgcg tggataagct gcccgcgacg
    11641 ttcttcgtcg ctgatccgac actggatctg caaaatgatt ttttcctggg cggtaacaaa
    11701 agcaatcgca tcgccagaat gaatttccgg ttcctgttcc gctatatatt caatatcctg
    11761 cggccagata ttacggccac gaataataat cagatcttta atgcgtccgg tgacatacag
    11821 ataaccgtcc agcagataac cgaggtcgcc ggtgtctaac cagcccgtcg cggcaatctc
    11881 gtcttgcgaa acctggtcgc caaagtaacc gctcatcaga ctgggaccgg agatgcaaat
    11941 atggcctacc acacgttccg cgaccggcat acctgcttca ttgcggattt caataccatg
    12001 ttccggcaac gctttgccgc agttgacgaa agtcgatacg gcgcgtgtct ctgcacccgg
    12061 cgcgacggct ttaccctgat attcgaggat gtcgcgatcc acttcgttaa ccacaacccc
    12121 ggaggcttca tcagagaagc tgacagccag cgcattttct gccagtccgt agcacggcat
    12181 gaaagtttta ttgtcaaagt taacctgacg gaaacattca gcgaattgat ggagttgttc
    12241 tgcggagatc ggctcagcac caataccagc gacgcgccag caggaaagat ccagttcagc
    12301 gagatctttt tcattcacgc ggcgttggca caattcatag ccaaacggcg gcgcaacgga
    12361 aacggtgccg cgatttttac tgatcaattt aagccattgc aaaggacgca tggcaaaatc
    12421 ctgagtgcgc aaataatcta ctgaaagttg cgtggcgacg gggggcagga gaaagccgac
    12481 cagtcccata tcatggtaga aaggctgcca ggagacgcag cggtcgctag ggcgtaattt
    12541 aatgccatcg tggcttatag cacgtagatt agccatcact tcgcgatggg tgataatgac
    12601 gccacgggga aaacgggtgc tgccggaggt gtactggagg taggcgatat cgttcggaac
    12661 tggacgctgg agcgcaacat cggcttccgg taatgcctta aaccaagcgt ggcttaaaac
    12721 atgtaattcg gggttgttat gcgtcgcggc attgaccagt ggcaaccact catcaccagt
    12781 gataatggct gcgggctggc aacttgccag taaaccctgg agtttggcgc tccaggaatc
    12841 ccgctgaccg acgcccattg gaatcgccaa cgggacggcg actaagccag catactgaca
    12901 ggcaaaaaaa gcctctacaa acccgctact cgtttcggca atcagcgcca cgcgttcgcc
    12961 ttttttcagg ttcagcgata acaaccgctt cgcaccagct tcggcacgcg tttttaatgt
    13021 ctgatattcc agttgatctt caagttggca acgtctgtca taaaaattca ttccggctct
    13081 actcagagcg gcgtagtcca aagcatcaac cagcgttgga aaatcggcat agcgcatagg
    13141 tagggaatgc gtaaagattt tattagacat atacaccacg aaaaaattgg atatcaaaaa
    13201 taaaatgaca ataatgcggt ggattatata ggcataaaag at
//
LOCUS       DAZPAB010000101        13192 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZPAB010000101 DAZPAB010000000
VERSION     DAZPAB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13192
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.101"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..112)
                     /locus_tag="ACHUPG_002587"
     CDS             complement(<1..112)
                     /locus_tag="ACHUPG_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 domain-containing protein"
                     /protein_id="HFR0862149.1"
                     /translation="MLKQKIKTIFEALPYIMLTYWLIDSFFAFNKYDWMLE"
     gene            431..1012
                     /gene="mdaB"
                     /locus_tag="ACHUPG_002588"
     CDS             431..1012
                     /gene="mdaB"
                     /locus_tag="ACHUPG_002588"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR0862150.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            1043..1357
                     /locus_tag="ACHUPG_002589"
     CDS             1043..1357
                     /locus_tag="ACHUPG_002589"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR0862151.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1405..3297)
                     /gene="parE"
                     /locus_tag="ACHUPG_002590"
     CDS             complement(1405..3297)
                     /gene="parE"
                     /locus_tag="ACHUPG_002590"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR0862152.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3326..3907)
                     /gene="yqiA"
                     /locus_tag="ACHUPG_002591"
     CDS             complement(3326..3907)
                     /gene="yqiA"
                     /locus_tag="ACHUPG_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR0862153.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3907..4734)
                     /gene="cpdA"
                     /locus_tag="ACHUPG_002592"
     CDS             complement(3907..4734)
                     /gene="cpdA"
                     /locus_tag="ACHUPG_002592"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR0862154.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4759..5181)
                     /locus_tag="ACHUPG_002593"
     CDS             complement(4759..5181)
                     /locus_tag="ACHUPG_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR0862155.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5182..5811)
                     /gene="nudF"
                     /locus_tag="ACHUPG_002594"
     CDS             complement(5182..5811)
                     /gene="nudF"
                     /locus_tag="ACHUPG_002594"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR0862156.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6016..7497
                     /gene="tolC"
                     /locus_tag="ACHUPG_002595"
     CDS             6016..7497
                     /gene="tolC"
                     /locus_tag="ACHUPG_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR0862157.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7645..8316
                     /locus_tag="ACHUPG_002596"
     CDS             7645..8316
                     /locus_tag="ACHUPG_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR0862158.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8322..9482
                     /locus_tag="ACHUPG_002597"
     CDS             8322..9482
                     /locus_tag="ACHUPG_002597"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR0862159.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9520..10308)
                     /gene="ygiD"
                     /locus_tag="ACHUPG_002598"
     CDS             complement(9520..10308)
                     /gene="ygiD"
                     /locus_tag="ACHUPG_002598"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR0862160.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10451..11224
                     /gene="zupT"
                     /locus_tag="ACHUPG_002599"
     CDS             10451..11224
                     /gene="zupT"
                     /locus_tag="ACHUPG_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR0862161.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11282..11452)
                     /gene="yqiD"
                     /locus_tag="ACHUPG_002600"
     CDS             complement(11282..11452)
                     /gene="yqiD"
                     /locus_tag="ACHUPG_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR0862162.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11713..12366)
                     /gene="ribB"
                     /locus_tag="ACHUPG_002601"
     CDS             complement(11713..12366)
                     /gene="ribB"
                     /locus_tag="ACHUPG_002601"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR0862163.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(12470..12618)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12740..13030
                     /gene="ubiK"
                     /locus_tag="ACHUPG_002602"
     CDS             12740..13030
                     /gene="ubiK"
                     /locus_tag="ACHUPG_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR0862164.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT         3370 a         3507 c         3181 g         3134 t
ORIGIN      
        1 actcaagcat ccagtcatac ttattgaaag caaaaaaaga atctattaac caataagtaa
       61 gcattatata tggcaatgct tcaaatattg ttttaatttt ctgctttaac atatatcagt
      121 acgctcataa aaatccattt ataattatca acatgacacg ttgatatttt catcttttac
      181 ttattcaaag ccatattttc aatccccaaa aaaaccgaac caaacgtgta aattattccg
      241 gtgcgagtaa gaattatgag gaatggctat cagtattgtc attttcagaa aatatttatc
      301 ctgcatcggt gagtcagagc tagatcagac ttttgctaaa ttcgcaaaag actttgcaca
      361 ttttgctaat ttcacagtac cgctctgtga cgtactatag tcgacaaacg tctcaccttg
      421 agattaaaaa atgagcaaca tcctgattat caacggcgcg aaaaaattcg cccactccaa
      481 tggtcaactg aacgacaccc tgaccgaagt cgcggatggc acactgcgcg acctcgggca
      541 tgatgtccgc atcgttcgcg ccgacagcga ctacaatgtc aaagcggaag tacaaaactt
      601 tctctgggct gatgtggtga tctggcagat gccaggctgg tggatgggcg cgccgtggac
      661 agtgaaaaaa tatattgatg atgtattcac cgaaggtcac gggactctgt atgccagcga
      721 tggtcgtacc cgcaaagatc cgtcgaaaaa atacggttcc ggcagcctgg tacaggacaa
      781 aaaatatatg ctttctctaa cctggaacgc accaatggaa gccttcaccg aaaaagatca
      841 gttcttccac ggcgttggcg ttgacggtgt gtatctgccg ttccataaag caaaccaatt
      901 cctcggtatg gaaccgctgc cgacatttat cgctaatgac gtgataaaaa tgcctgatgt
      961 tccccgctat actgaagaat atcgcaagca tcttgtggaa atttttggtt aactagagct
     1021 caggctttag aaggagttaa ccatgcttac cgtaatcgca gaaatccgta ctcgtcctgg
     1081 tcaacatcac cgtcaggcgg tattggatca gtttgctaaa atcgttccaa ccgtactgaa
     1141 agaagaaggt tgccacggct atgcgccaat ggtggattgc gctgctggcg tgagtttcca
     1201 gtctatggca ccggattcta tcgtgatgat tgagcagtgg gaaagcatcg cgcaccttga
     1261 agcgcatctg caaaccccgc acatgaaggc gtacagcgaa gccgtaaaag gcgacgtgct
     1321 ggagatgaat atccgtattc tgcagccagg gatttaatcc tgccttgttt gcccggccat
     1381 cctgaccggg caatgttctt tcctttaaac ctcaatctcc gccatgtcgc ctttctcttg
     1441 caaccagttg cggcgatctt ccgagcgttt cttcgccagc agcatatcca tcatcgcgtc
     1501 agtacgctga tcgtcttcat catcgatagt caactgcacc agacggcgag tgttcggatc
     1561 aagcgtggtt tcacgcaatt gcatcgggtt catttccccc agacctttaa aacgctggac
     1621 gttcggcttg cctttcttgc gttttaattg ctcaagtacg ccctctttct cttcttccgt
     1681 cagcgcgtaa taaacctctt tcccgagatc aatacggtag agcggtggca gtgcgacgta
     1741 aacgtgaccg tgtttcacca acgcgcggaa gtgttttacg aacaaagcgc agagcagcgt
     1801 ggcaatgtgc agaccatcag agtccgcatc cgcgaggata cagattttgc cataacgaag
     1861 ctggctcaga tcgtcgctgt caggatcgat accgatcgct accgaaatat catgcacttc
     1921 ctgcgaggcc agcacttcgt cggaagaaac ttcccaggtg ttaaggatct tacctttcag
     1981 cggcatgatc gcctgatatt cacgatcgcg cgcctgcttg gcagatccgc ctgcggagtc
     2041 accttccacg aggaacagtt cggtacggtt aaggccctgc gcggtacaat cagccagttt
     2101 gccaggcagc gccgggccgc tggtcagctt tttacgcacc acttttttag ccgcacgcat
     2161 acgacgctgg gcgctggaaa tcgccatctc tgccagcaac tccgccgcct gaacgttctg
     2221 gttcagccac aggatgaagg catctttcac cacgcccgaa acgaatgccg cacactgacg
     2281 cgaagagaga cgctctttgg tctgcccggc aaactgcgga tcctgcattt taaccgacag
     2341 aacataggcg cagcgatccc agatatcttc cgccgacagc ttcacgccgc gcggcagaat
     2401 gttgcggtat tcacagaact cacgcatcgc gtccaacagg ccctgacgca gaccattaac
     2461 atgggtaccg ccctgcatcg ttgggataag gttgacgtag ctttcggtca gcagttcacc
     2521 gccttccggc agccacagta gcgcccagtc cacagcttca gtatcgccag cgaaattacc
     2581 gataaacggt ttttctggca gcgtcggtaa accgtttacc gcttccgcca ggtaatcatt
     2641 cagaccgtcc tgatagcacc agcgctgttc ggtgttgttg atctcatctt taaaggtaat
     2701 ctcaacgccg gggcacagta ctgctttggc cttcagcaca tgcgtcaggc gtgaaacaga
     2761 aaaacgcggg ctgtcaaaga aggtttcatc cggccagaag tgcacgctgg taccggtatt
     2821 gcgtttaccg caagtgccga caacctgtaa atcctgcacc ttttcgccat tttcaaaggc
     2881 gatgttataa acctgaccat cgcggcgcac gttaacttct acgcgcttcg acaaggcgtt
     2941 aactaccgaa atccccacgc catgcaggcc gccagagaac tggtaatttt tgttagagaa
     3001 tttaccgcct gcatgcagac ggcaaagaat cagttcaacc gccggtactc cttcttccgg
     3061 gtgaatatcc accggcatcc cgcgcccatc gtcaatcact tctaacgact ggtcagcatg
     3121 taagattaca tccacgcgtt ttgcgtgacc cgccagtgct tcatcgacac tgttatcaat
     3181 aacttcttgc cccaaatggt tagggcgagt ggtatcggta tacatccccg gacggcggcg
     3241 aaccggctca agcccggtga gtacctcaat ggcatcagcg ttataagttt gcgtcatggt
     3301 ttaagttagc aattcgagtt gatcgtcaga gatggtgcag accaagaaaa tcgacgatcg
     3361 ggttgaaata atcttcgaag cccgtgaatg cgtggttgcc gccttctatg acagtctggc
     3421 ggcaggaagc atagtacgcc accgcctggc ggtaatccag cacttcatct cccgtctgtt
     3481 gcagcagcca gatcaaatcc ggcgcttcca gcgggtcaat ctgcatgact ttgagatcgt
     3541 aaatatggcg tgactctagc acatattgct gcccggtgta ggggttctcg ttctgaccga
     3601 gatagtccgt cagcaattca aacgggcgta ccgccgggtt taccaccact gcgggcagca
     3661 taaaacattg cgacaaccag gtggcgtaat atccccccag tgacgaaccg acaataccca
     3721 gcgaatcgcc gccatgttcc aggacaatgg attccagcag ttctgccgcg tcggaaggat
     3781 acggcggcaa ctgcggaatg atcatctcaa cgtcagggtg atgttccgcc agccagtttt
     3841 ttaacaagct cgcttttgca gagcgcggcg agctgttgaa accgtgtaaa taaagaagcg
     3901 tagacatcag tagccttctg aagcggtatc aggttggaaa cgtgtgtccg ccaggcgatg
     3961 cacctcggtg gtcagcgtgc catcagcatg taactcgaga gtacgccagc cgggcgcgat
     4021 agtatccagc gtaaagttgg aacagtgcgg cttaaactgc acacaggtcg acggcgttgc
     4081 cagcaggcgg cgaccgttcc agtcgagatc cagctcctga tgaatatgac cgcacagcaa
     4141 gtatttgacg tgcggaaact tcgccagcac ggtatccagt tcgcccgcgt tacgcagact
     4201 gtgttgatcg agccaactac aacccgcagg tagcggatga tgatgcagca gcagcaacgt
     4261 atggcgttct ggcgcatcgg ccagtttacg ttccagccac tcaagctgaa actcgctcag
     4321 ctcaccgtgc ggcacgccaa acacctggct atccagcaac aggatttgcc attgctcacc
     4381 aataaacacc cgcttcgccg gggagatacc cgcatcctgt aacgcgctgt acatcgcggg
     4441 ctggaaatcg tggttgcccg gcagccagac gcagggcgca cgaaaacttg cgatgccttc
     4501 agcgaaatgc tgataggccg cagaggattg atcctgcgct aaatcacctg tcgcgacaat
     4561 caggtcgaat tcgtgctggt gtggacgaat cgcctccagc accgcctggt agctttccca
     4621 ggtgtttacc cctaacaggg cttcgtgctt ttgtgcaaac aggtgagtat cggtaatttg
     4681 taaaatcctt cctctggcct caccagccag aggaagggtt aacaggcttt ccaaatggtg
     4741 tccttaggtt tcacgacgct agtaaaccgg aatcgccatc gctccatgtg ctaaacagta
     4801 tcgcaaccag tccgctaaaa actggttaat ttgatgcttt tcgtcgcgtt gatgcaactt
     4861 tttattagga taatcatacc gcgctttgaa gcgaaaaatc tgctggcttg aacacacttc
     4921 agccaccatc gcgtcatgat acagacgcac cgtcattgac ggaaggctcc agtaactgat
     4981 cgcgggcgca gtctgttcta ttgtcaccag ggtagtgtat cgggtcgatt ccacaatcgt
     5041 cagccgatat tgtgcgtttg ccacctgata gcttacagtt tcgccgggtg cgtcattgcg
     5101 cggtaacaaa cggcgcaatt gtgaaaagtt catctcgcac aggcgcatca tttcaggaaa
     5161 gtcaggtgtg taacgcttca tttatgccca ctcatttttt aacgcttgat gatgcagctg
     5221 cagccattgc aaagcgatga ccgacgctgc gttgtcgatt ttcccctctt ctacccactg
     5281 gtatgcctgt tcccggctta ccacatgaac gcgaatatct tcgttttcat cagccagacc
     5341 atgaataccg cttgcggtcg tggcgtccac ttcgcccacc ataattgacg aacgctcact
     5401 ggtgcccccc gggcttgcca ggaaacttaa caccggtttg gtccgtttga ctatcagtcc
     5461 cgcctcttca atcgcttcgc gacgggcaac atcttccaca ctttcaccct cttcaatcat
     5521 cccggcaacc atctccagta gccagggggt ttcgctggtg tcgtacgcgg caatccgaat
     5581 ctgctcaatc agcacaactt catcacgcac tgggtcaaag ggtagcaaga ctgcggcgtg
     5641 accgcgctca aaaatttccc gccgtacctc atgactcatt tgcccgttga atagacgatg
     5701 acgaaatcta taaagatcta atgaaaaaaa gccgcgataa agtgtttctc gtgcaataat
     5761 ttctacatcg tttttgccaa atgtaacggg caggttgtct ggcttaagca ttgttaatgt
     5821 cctagcacta atagtgaatt aaatgtgaat ttcagcgacg tttgactgcc gtttgagcag
     5881 tcatatgtta aattgaggca cattaacgcc ctatggcacg taacgccaac cttttgcggt
     5941 agcggcttct gctagaatcc gcaataattt tacagtttga tcgcgctaaa tactgcttca
     6001 ccacaaggaa tgcaaatgaa gaaattgctc cccattctta tcggcctgag cctttctggg
     6061 ttcagttcgt tgagccaggc cgagaacctg atgcaagttt atcagcaagc acgccttagt
     6121 aacccggaat tgcgtaagtc tgccgccgat cgtgatgctg cctttgaaaa aattaatgaa
     6181 gcgcgcagtc cattactgcc acagctaggt ttaggtgcag attacaccta tagcaacggc
     6241 taccgcgacg cgaacggcat caactcgaac gcgaccagtg cgtccctgca gttaactcaa
     6301 tccatttttg atatgtcgaa atggcgtgcg ttaacgctgc aggaaaaagc agcagggatt
     6361 caggacgtca catatcagac cgatcagcaa accttgatcc tcaacaccgc gaccgcttat
     6421 ttcaacgtgt tgaatgctat tgacgttctt tcctatacac aggcacaaaa agaagcgatc
     6481 taccgtcaat tagatcaaac cacccaacgt tttaacgtgg gcctggtagc gatcaccgac
     6541 gtgcagaacg cccgcgcgca gtacgatacc gtgctggcga acgaagtgac cgcacgtaat
     6601 aaccttgata acgcggtaga gcagctgcgc cagatcaccg gtaactacta tccggaactg
     6661 gcggcgctga atgtcgaaaa ctttaaaacc gacaaaccac agccggttaa cgcgctgctg
     6721 aaagaagccg aaaaacgcaa cctgtcgctg ttacaggcac gcttgagcca ggacctggag
     6781 cgcgagcaaa ttcgccaggc gcaggatggt cacttaccga ctctggattt aacggcttct
     6841 accgggattt ctgacacctc ttatagcggt tcgaaaaccc gtggtgccgc tggtacccag
     6901 tatgacgata gcaatatggg ccagaacaaa gttggcctga gcttctcgct gccgatttat
     6961 cagggcggaa tggttaactc gcaggtgaaa caggcacagt acaactttgt tggtgccagt
     7021 gagcaactgg aaagcgcaca tcgtagcgtc gtgcaaaccg tacgttcctc cttcaacaac
     7081 attaatgctt ctatcagtag tattaacgcc tacaaacaag ccgtagtttc cgcgcaaagc
     7141 tcattagacg cgatggaagc gggctactcg gtcggtacgc gtaccattgt tgatgtgttg
     7201 gatgcgacca ccacgctgta caacgccaag caagagctgg cgaatgcgcg ttataactac
     7261 ctgattaatc agctgaatat taagtcagcc ctgggtacgt tgaacgagca ggatttgctg
     7321 gcactgaaca atgcgctgag caaaccggtt tccactaatc cggaaaacgt tgccccgcaa
     7381 acgccggaac agaatgctat tgctgatggt tatgcgcctg atagcccggc acccgtcgtt
     7441 cagcaaacat ccgcacgcac taccaccagt aacggtcata accctttccg taactgatga
     7501 cgacgacggg gcttcggccc cgtctgaacg taaggcaacg taaagatacg ggttatctgc
     7561 cgcattcttc ccccttctcg cttcaatttc gaccagccat cctctattct gatgggtatt
     7621 taccactggt cccggaagac aaaaatgaaa cggacaaaat ccatacgcca cgcatcgttc
     7681 cgcaaaaact ggagcgcacg ccatctgaca ccagtcgctc tcgcggttgc cactgttttt
     7741 atgctggctg gctgtgaaaa gagtgatgaa acagtgtctc tctatcaaaa tgctgacgac
     7801 tgttcagctg caaacccagg caaaagcgcc gaatgtacca ccgcgtacaa caatgcgctg
     7861 aaagaagccg aacgtactgc gccgaaatac gccacccgtg aagactgtgt tgctgaattt
     7921 ggtgaaggtc agtgtcagca agcaccagcc caggctggca tggcaccaga aaaccaggcg
     7981 caggctcagc aatccagcgg gagtttctgg atgccgctga tggccggtta catgatgggg
     8041 cgtctgatgg gcggcggcgc gggatttgca cagcagccgc tgttctcctc gaaaaacccg
     8101 gccagtccgg cttacggtaa atataccgac gcgacgggta aaaactatgg cgcagcccag
     8161 ccaggccgca ccatgaccgt accgaagacg gcaatggcac caaaaccggc gaccaccact
     8221 accgttaccc gtggcggttt tggtgaatct gttgccaaac aaagcactat gcagcgtagc
     8281 gcaaccggca cctcttctcg ttcaatgggt ggctgatacc gatggaaaga gtcagtatta
     8341 ccgagcgccc ggactggcgt gagaaagccc acgaatacgg tttcaatttt cacaccatgt
     8401 acggcgagcc gtactggtgt gaagatgctt actacaagtt gaccctcgcc caggttgaaa
     8461 agctggaaga agtcaccgcc gaactgcacc agatgtgcct gaaagtggtg gaaaaagtga
     8521 tcgccagcga tgagctgatg accaaattcc gcataccaaa acacacctgg agttttgtgc
     8581 gtcaatcatg gctgactcac cagccatcgc tttattcgcg tcttgatctg gcgtgggatg
     8641 gcactggcga acctaaactt ctggaaaata acgccgatac gccaacgtca ctatacgagg
     8701 cggcgttctt tcagtggatc tggctggaag atcagcttaa cgccggtaac ttgccggagg
     8761 gcagcgacca gtttaacagt ctgcaagaaa aactgatcga tcgcttcgtt gagctgcgtg
     8821 aacagtatgg cttccagttg ctgcatctca cctgctgtcg cgacacggtg gaagatcgcg
     8881 gaaccattca gtatttgcag gactgcgcaa ccgaagctga aatcgccacc gagttcctct
     8941 acatcgacga gataggttta ggcgaaaaag gccagttcac ggatttacag gatcaggtga
     9001 tttctaacct gttcaagctt tatccgtggg aatttatgct gcgcgaaatg ttctccacca
     9061 agctggaaga tgctggcgta cgctggctgg aaccggcgtg gaagagcatt atctccaaca
     9121 aggcacttct accgctactg tgggagatgt tcccgaatca cccgaacctg ctgcccgctt
     9181 attttgcgga agatgatcat ccgcaaatgg aaaaatatgt ggttaaaccg atcttctccc
     9241 gtgaaggcgc aaacgtgtcg atcattgaga acggcaaaac cattgaagca gcggaaggtc
     9301 cgtatggcga agaagggatg attgttcagc aattccaccc gttaccgaaa ttcggcgaca
     9361 gctatatgct gattggtagc tggctggtga acgatcaacc tgccggaatt ggcattcgtg
     9421 aagaccgtgc gttgatcacc caggatatgt ctcggtttta tccacatatt tttgttgaat
     9481 aagccacgat accggatggc actcgccatc cggtaattgt tagcctatct gcaccgacag
     9541 catacttaaa ctgcccattt ctataccatc gaccggaatg gtaattggct cctgcccatc
     9601 ccacacacct aacacataca acaacggcag atagtgctct ggcgttgggt tcgataacgc
     9661 gccaccttca tggtcgagat aattcaccag aggatgctgc tctaccggcc cttgccacgt
     9721 cagattcgct ttcacatact cattaaacga cgtcgcccac ggatacggtg aactatcacc
     9781 gtgccacttc actgtgcgca ggttatgcac cacgttaccg ctggcgacca acattattcc
     9841 ttcatcacgc agcgccgcga gtttgcgccc catttcgaaa tgccaggcgg caggtttgct
     9901 actgtcgata ctcaactgca ccatagggat atcggcgtca ggatacatct taatcagcac
     9961 gccccacgag ccgtggtcaa agccccaggc ttctttatcc agcgtcaccg ggatcggcgc
    10021 taacagctca accagacgct gtgccagcgc aggcgaaccc ggagcaggat aatgcgtatc
    10081 gtacagcgcc tgcgggaagc caccaaagtc atgaatcgtg ggcggcgtct ccatcgcggt
    10141 cactcctgtt ccacgggtaa accagtgagc cgaaaccacc acaatcgctt gcgggcgtgg
    10201 caatgtcatc cccaacttct gccagctgcg ggtatacaaa ttatcttcca gcacgttcat
    10261 cggactaccg tgacctaaaa acaatgctgg catacgtgtt gaagacatga tgatatcctt
    10321 aactaaaggt gtcattttga tatcctcaca atacgcttgt tcggcggagt aagaacccgg
    10381 ataacaatga tgatgatcat cagttatttt gacgatctgc ctgaaggtga agatttataa
    10441 ggagttgtcg atgtcagtac ctctcattct gaccatactg gcgggggcag ccacgtttat
    10501 tggcgcgttt ctcggcgttc tcggacaaaa accctcgaac cgcttactgg cgttttcgct
    10561 aggttttgcg gcggggatca tgttgctcat ctcattaatg gaaatgcttc ctgccgcact
    10621 agcagctgaa ggaatgtcgc cggtgttggg ttatggaatg tttatcttcg gtctgcttgg
    10681 ctactttggc ctggaccgca tgttgccgca tgcgcatccg caggatttaa tgcaaaaatc
    10741 ggtgcagctg ttgccaaaat cgatcaagcg cacagccatt ctgctcactc tcggcatcag
    10801 tctgcataac ttcccggaag ggattgccac ctttgtcacg gcgagcagca acctggagct
    10861 gggatttggc atcgcactgg ccgtcgcgtt gcacaatatc cctgaaggtc tggcagtggc
    10921 aggcccggtt tatgcggcaa cggggtctaa acgcaccgcg attctgtggg cggggatttc
    10981 tggactagca gaaattcttg gtggtgtgct ggcgtggtta atcctcggta gcatgatttc
    11041 cccggtggtc atggcggcaa tcatggcggc ggttgcagga attatggtgg cgctctcggt
    11101 tgatgaatta atgccgctcg ccaaagagat tgaccctaat aataacccca gctatggcgt
    11161 actatgtgga atgtcagtga tgggattcag tttagtgctg ctacaaacgg cgggaattgg
    11221 ttaaaaacaa catcagaagt tgtccagcac atggacaact tctgccagct tcacgaaagc
    11281 cttattctac cttttcggct gtctcttctc tcgtactgtt taagtatttg ttccgcgctt
    11341 caagcagtgc gtctctgtcc tggcgaaacg cccgacaata acgtttcaaa tgcaggaaat
    11401 aacccaccac aaccagagca atcaatacaa tccagtacca ggcgataaac atccttttac
    11461 ccttaatatt taacaatact aaacatttag cgcataaatt tcacatatcc tttttcggat
    11521 atattcatat ggtcgcataa taacggaaca attatatgat tttgatcata atgaaaaaga
    11581 atgcctgata ttaatgaaat tttctcatag aaagagaagc ggaattaaca tcttgcatag
    11641 cagggcaaaa taaaaaccga agccagctac aacttcggtt tcttgattaa ggcagtaatt
    11701 aagcagcggt tttcagctgg ctttacgctc atgtgcctga cggtatgcca ccaggtcttc
    11761 aatagtcacg agcgccatat tgtgtttatt ggcaaactca atgcactctg gtgcacgcgc
    11821 catcgtgcca tcgtcattag tcagctcaca cagcacacca gccggtttaa agcctgccag
    11881 cgtcatcaaa tcaatagtcg cttcagtatg accgccacgc gtcagtacac cacctgcctg
    11941 cgcgcgaagt gggaaaacgt ggcccgggcg attcagatca gaaggttttg cgccatcggc
    12001 aatcgctgcg cgaacggtcg taatacgatc agcggcagaa acaccggtag tcacaccttc
    12061 agccgcttca atggtcacgg taaaaccggt gccataggcg ctggtgttat tttctaccat
    12121 cattggcaga tcaagttgtt tacggcgatc ttcagtgatg cacaggcaaa caataccgct
    12181 accgtggcga atggtcagcg ccatctgctc aacggtcatg gtttctgccg ggaagatcat
    12241 atcgccttcg ttttcacggt cttcatcatc aagcaccatt acaccgcgtc cttcacgcag
    12301 cgcagccagt gcattttcaa cacgttcgaa aggcgtacca aaagaggaaa gtagcgtctg
    12361 attcatggta aaaaaacctc actaaaatta tggttaccag aatcagggca gtcttaggag
    12421 tggcggcata tagccaaaat aacgtgagcg ggtccatgcc cgacagaatc gttactctct
    12481 cccatccgga ctctaaccgt cggccccgga attacaccgg atctgctgtc ctttgagttc
    12541 gcacccaaag cgctcgcggg ctttcaactg agttgattta ccgccggtgg ggaatttcgc
    12601 cccgccctga gaataagcgg attcactata acgctaatga ttagcggcag caacgcatag
    12661 cttcacataa ttctggttta tgacttaccc ttatcgcact acaatggtac tcaacaccta
    12721 tcagtacagg gaaactacaa tgattgaccc gaaaaaaatt gagcaaatcg ctcgccaggt
    12781 tcacgaatca atgcctaaag gaatcaggga gttcggggaa gatgtggaga aaaaaatccg
    12841 ccaaacccta caagctcagc tgacgcgcct cgaccttgta agccgtgaag agttcgacgt
    12901 ccaaacgcaa gtcctgttac ggactcgtga gaaactggcg ctgcttgaac aaagaatcag
    12961 cgaactggaa aatcgcagta ctgagatcaa aaagcagcca gatccagaga ctctcccccc
    13021 aacgctgtaa acatttgtct ttaatgcact gtaaattatg aattaatcct ctaatttaca
    13081 gtgttttcta ttacattatc gcttaatccc attcagagta tcatcacaaa aatgaatgat
    13141 tgcaattgaa aatataattg ttatgtattc tttaagtttt acttaatcga ta
//
LOCUS       DAZPAB010000102        13177 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZPAB010000102 DAZPAB010000000
VERSION     DAZPAB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.102"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..1177)
                     /locus_tag="ACHUPG_002603"
     CDS             complement(<1..1177)
                     /locus_tag="ACHUPG_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0862165.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     "
     gene            complement(1376..1460)
                     /locus_tag="ACHUPG_002604"
     tRNA            complement(1376..1460)
                     /locus_tag="ACHUPG_002604"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1424..1426),aa:Leu,seq:caa)
     gene            1656..2675
                     /gene="ahr"
                     /locus_tag="ACHUPG_002605"
     CDS             1656..2675
                     /gene="ahr"
                     /locus_tag="ACHUPG_002605"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR0862166.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2804..4306
                     /locus_tag="ACHUPG_002606"
     CDS             2804..4306
                     /locus_tag="ACHUPG_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR0862167.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="ACHUPG_002607"
     CDS             complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="ACHUPG_002607"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR0862168.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="ACHUPG_002608"
     CDS             complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="ACHUPG_002608"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR0862169.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6670..6720)
                     /gene="ytgA"
                     /locus_tag="ACHUPG_002609"
     CDS             complement(6670..6720)
                     /gene="ytgA"
                     /locus_tag="ACHUPG_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR0862170.1"
                     /translation="MSIIILATISTTQENK"
     gene            6874..8385
                     /gene="pepA"
                     /locus_tag="ACHUPG_002610"
     CDS             6874..8385
                     /gene="pepA"
                     /locus_tag="ACHUPG_002610"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR0862171.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8519..8962
                     /gene="holC"
                     /locus_tag="ACHUPG_002611"
     CDS             8519..8962
                     /gene="holC"
                     /locus_tag="ACHUPG_002611"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR0862172.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8962..11817
                     /gene="valS"
                     /locus_tag="ACHUPG_002612"
     CDS             8962..11817
                     /gene="valS"
                     /locus_tag="ACHUPG_002612"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR0862173.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11871..13067)
                     /locus_tag="ACHUPG_002613"
     CDS             complement(11871..13067)
                     /locus_tag="ACHUPG_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFR0862174.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
BASE COUNT         3399 a         3402 c         3415 g         2961 t
ORIGIN      
        1 acatcaacct acgctcccca agatgttccg ccttatggat gtaagctgct cgcacagagc
       61 tacgctcctg gtgactcatc tgccgctcta ccgcatccct cgaccataat cccgactcaa
      121 tcaacgaact acaagccatc gtcctgaagc catgcccaca aacctcgacc ttcgtgtcat
      181 accccattac gcgcaaagcc ttgttcaccg tattctcact catcggctta cgtggatcgt
      241 gatcgcccac aaaaatcagc tctcgatttc cgctcatgct tttgatcttt tccaaaatac
      301 ttagggcctg ccgcgacaaa ggtacaaggt gtggtgtccg catcttcgag ccacgctgag
      361 aatgcttgac tccttccagt tgctcacgct cacctgggat tgtccacata gccgtttcaa
      421 aatctatttc tgaccagcgg gcaaaacgca gctcgctcga acggatgaaa accaataaag
      481 tgagttcaac cgccagtcga gttaacggtc tgccagaata gtgatctatg cgatgaagta
      541 attcaggtat acggttcagt tcaagagctg cacggtgctg tcttttcgcc gtagcaaccg
      601 cacctgcaat ctcttgcgcg gggttgtagt cgattaagcc gctctgaacg gcaaagcgca
      661 taatcgcggt agtacgctgc tgtaaacggg cggcaacttc gagccgcccg gatgactcga
      721 ctgctttaat gggtacaaga agatcccgcg tcttcagttc cgcaatgttc cgcttaccga
      781 tagcagtaaa gagattatcc tccagacttt tcaaaacacg agcgctatgc gatgccgacc
      841 acttctgatt gctggcgtgc cagtctctgg cgactacttc aaacgttatc gcctcttgtt
      901 cctgttctac cttaacagct ttcttgtttt cactgggatc gacaccatta gctaaaagct
      961 tacgagcctc atcccggcgt gccctggcat ccgccaacga cacttcgggg tatttcccca
     1021 atgccagcat cttctctttg ccgccaaagc gataacgtag ccgccagtat ttggagccgt
     1081 tagggtgaac cagcaaaacc atgccttcac cgtcggtcag cttataggct tttgcttcag
     1141 gcttagccga acgaaccttc acatcactca gagccatgat gagtatcctt tcaagggttc
     1201 tgtgtgggta caaacattat cgaaccggga aatacccgca gttgtacccg catcagtaag
     1261 ttgatgtaga ttgaatcagg ttgacttagg ttgagtgaaa aagcgaggaa agccttgcgg
     1321 atactggatt ttaggcacaa aaaaagacgt ccgttgacgt ctattgatgt tccgatggtg
     1381 ccgaaggccg gactcgaacc ggcacgtatt tctacggttg attttgaatc aactgcgtct
     1441 accgatttcg ccacttcggc actgaagagg tatgcggaaa acgttgtgga ttatacctgt
     1501 cgcacgccac catgcaagcg ccagcttgca cccacctcgc taagtgctga aattttcagc
     1561 attacactca gcgatatgta acctttgtca cactccaggc accccgccct gccatgctct
     1621 acacttccca aacaacacca gagaaggccc caaaaatgtc gatgataaaa agctatgccg
     1681 caaaagaagc gggcggcgaa ctggaagttt atgagtacga tcccggtgag ctgaagccac
     1741 aagatgttga agtgcaggtg gattactgcg ggatttgtca ttccgatctg tcgatgatcg
     1801 ataacgaatg gggattttca caatatccgc tggttgccgg gcatgaggtg attgggcgcg
     1861 tagtggcact cgggagcgcc gcgcaggata aaggtttgca ggtcggtcag cgtgtcggga
     1921 ttggctggac ggcgcgtagc tgtggtcact gcgacgcctg tattagcggt aatcagatca
     1981 actgcgagca aggtgcggtg ccgacaatta tgaatcgcgg tggctttgcc gagaagttgc
     2041 gtgcggactg gcaatgggtg attccactgc cagaaaatat tgatatcgag tccgccgggc
     2101 cgctgttgtg cggcggtatc acggtcttta aaccactgtt gatgcaccat atcactgcta
     2161 ccagccgcgt tggggtaatt ggtattggcg ggctggggca tatcgctata aaacttctgc
     2221 acgcaatggg atgcgaggtg acggccttta gttctaatcc ggcgaaagag caggaagtac
     2281 tggcgatggg tgccgataaa gtggtgaata gccgcgatcc gcaggcactg aaagccctgt
     2341 cggggcagtt tgatctcatt atcaatactg tgaacgtcag cctcgactgg cagccttatt
     2401 ttgaggcgct gacctatggc ggtaatttcc atacggtcgg tgcggttctc acgccgctgt
     2461 ctgttccggc ctttacgtta attgcgggcg accgcagcat ctctggttct gctaccggca
     2521 cgccttatga gctgcgaaag ctgatgcgct ttgccgcccg cagcaaggtt gcgccgacaa
     2581 ccgaactgtt cccgatgtcg aaaattaacg acgccattca gcatgtacgc gatggtaaag
     2641 cgcgctaccg agtagtcctg aaagccgact tctgatccct ttgccgggtg ctgtcgcccg
     2701 gcacttaaat ctctttggaa ggcgtctcgc aatctgctga atttagcggt gaagagtgca
     2761 cagccgttgc ctatactcca ggcaggaaac tggaggaaat ctcatgagcg aacccctgtt
     2821 aattgcccgc acgccggaca cagaactgtt tttactgccg ggaatggcta accgtcacgg
     2881 gctgattact ggcgcaacgg ggacgggtaa aaccgttacg ctgcaaaaac tggcagagtc
     2941 attgtcggaa atcggcgtgc cggtgtttat ggctgatgtg aaaggcgatc tgaccggtat
     3001 cgcgcaggca ggaacggcgt cggaaaaact gctcacaagg cttaaaaata tcggcgtcaa
     3061 tgactggcaa ccgcatgcca atccggtggt ggtgtgggat atctttggcg agaaaggcca
     3121 cacggtgcgg gcgacggttt cggatctggg gccgctgttg ctggcgcgac tgttgaatct
     3181 caacgatgtg caatctggcg tgctgaatat tatcttccgc attgcggacg atcagggatt
     3241 gttgctgctc gactttaaag atttgcgggc gattacccag tacatcggcg ataacgccaa
     3301 atccttccag aatcagtacg gaaatatcag tagcgcatcg gttggtgcca tccagcgcgg
     3361 attactgtcg ctggaacagc aaggcgcagc acacttcttt ggtgagccga tgctggatat
     3421 caaagactgg atgcgcaccg atgccaacgg taaaggcgtt atcaatatcc tcagcgccga
     3481 gaaactttat cagatgccga aactgtacgc cgccagcctg ctgtggatgc tttcagagtt
     3541 gtatgaacaa ttgccggaag cgggcgatct ggagaaacca aaactggtgt ttttcttcga
     3601 cgaggcacat ctgctgttta acgatgcacc gcaggtactg ctggataaga ttgagcaggt
     3661 gataaggctt attcgctcaa aaggcgtggg cgtctggttc gtttcgcaaa acccgtctga
     3721 tattccggat aatgtgttcg gccagctcgg taatcgtgtt cagcacgctt tgcgggcttt
     3781 tacgccaaaa gatcagaaag cagtgaagac agcggcgcaa accatgcggg ccaatccggc
     3841 atttgatacc gaaaaggcaa tccaggaact ggggaccggc gaggcgttaa tctcgtttct
     3901 cgatgcaaaa ggaagtcctt ctgtggtgga acgggcgatg gtgatcgcac cttgttcgcg
     3961 aatggggccg gtgacggaag atgagcgtaa tggcctgatt aatcactccc cggtgtatgg
     4021 caaatatgaa gatgaggtgg accgcgagtc cgcctatgag atgctgcaaa aaggctttca
     4081 ggccagtacc gagcagcaaa ataatccccc cgtgaaaggg aaagaggtgg cggtggatga
     4141 cggtattctt ggtggattga aggatatttt gtttggcact accggaccac gcggcgggaa
     4201 gaaagatggt gtggtgcaaa caatggcgaa aagcgccgct cgccaggtga cgaatcagat
     4261 tgtacgcggg atgttgggga gtttgctggg ggggagaaaa agataagtca gacgtgatct
     4321 gtgcccctca ccctaaccct ctccccaaag gggcgagggg accgttcgaa ctcgtatttg
     4381 actttgtcaa caataataaa gggagctttc gctcccttgt tagattacga ttttctcatt
     4441 aacagccaca ggctgattaa gaagaagctg gcacttggca acagtgcgcc gatgatcggc
     4501 gggatgccgt aaaccaacgt cagcgggccg aagatctggt ccagtacgta gaagacaaaa
     4561 ccgaagctga tacctgtgac cacacgcacg cccatcggta cgctacgcag tgggccaaag
     4621 ataaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa gattttgctc
     4681 cacatgttga gctgataacg tccggcatcc tgaccgctcg acttcagata cttcacgtag
     4741 ttgtgcaaac cactgatgga gagtgcatcc gggtccagcg ccaccacgcc cagtttgtct
     4801 ggcgtgaggt cggttttcca ggtgccgctc accgtctgcg aaccggtaat ctgtttcgga
     4861 ttggtcagat cagattcatc aacctgcgac agacgccaga ctttatgttc cgggtcaaac
     4921 ttcgcggtag cggcataacg tacggattgc agacgacgat tctcgttaaa ggcataaata
     4981 ctgatgccac ctaacacctc gtcaccttta acccgctcaa tgtagacgaa gttgttgcca
     5041 tctttcgccc ataaaccttg ctgggtagag agcaacgagc cgccgtacat cgcctgcgca
     5101 cggtagttac gcgccatctg ttcgccctgc ggcgcgaccc attcgccaat cgccatcgtc
     5161 agcaagacca gtggaatggc ggttttcatc accgacagcg ctacctgcat acgggtaaaa
     5221 ccagaagcct gcatcaccac cagttcgctg cgctgcgcca gcatcccaag accaagcaac
     5281 gccccaagaa gagccgccat cgggaagaag atctgcacat ctttcggcac gctcagcaag
     5341 gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt cagctgatcg
     5401 acaaatttga taatgcccga cagcgacacc agcatgaaca gcgtcatcat gatggtggtg
     5461 aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac accgctcctt
     5521 tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg ttgagaacaa
     5581 tcgccaaagc cagataaatc aggttaacgg tccacatcca cagtgtcggg tccagcttac
     5641 ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaaaagtaga tacagcagca
     5701 tggctggcag catcgacagt acgcgtccct gacgcgggtt aaccacgctc aacggtacaa
     5761 ccataagtgc catcataaac acggtgaata ccaacgtgat acgccagttc agttctgcgc
     5821 gagcacgatc ggtgtcagtg ttccacaatg tgcgcatgtc catctggtcg gtatcgttcg
     5881 ggtcgagcgc caccgcctgg tgaccaatga tcgcctgata atcctggaaa tccgtaatgc
     5941 ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga gtgacgacct
     6001 gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca gaaggacgtg
     6061 cattaccttt tggtcgaatt tgtgcgagga acacatcttt gaaatcgctg ccgtcaacgc
     6121 tttcgatgaa cagcaccgag ctgccattag tcgcttgctg gaattgccct tgcgccagcg
     6181 ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga cgcgatgacc
     6241 acggtcccgc ccacatcacg ttaaccgccg cgacgattgc cgtgaatacc gcaaggatca
     6301 ttgccgcttt caccagaacc gctttgctca agccgcaggc atgcattacc gtaatttcac
     6361 tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt aatggcagga
     6421 taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga ttcgccggaa
     6481 tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag aagatcaaaa
     6541 gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga tatcttatga
     6601 ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc ttgtttcatg
     6661 gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa ttatactcaa
     6721 cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt taagattaac
     6781 acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat ctgtagccac
     6841 cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa aagcggtagc
     6901 ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg tcgcctttct
     6961 ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct gctacgccgg
     7021 ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc accatgttcc gaatgtactt
     7081 tccgagcgaa ttctccttat tggttgcggc aaagaacgtg agctggatga gcgtcagtac
     7141 aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcgat ggaagcggtc
     7201 tgctttctga ctgaactgca cgttaaaggc cgtaacaact actggaaagt gcgtcaggct
     7261 gtcgagacag caaaagagac gctctacagt ttcgatcagt tgaaaacgaa taagagcgaa
     7321 ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga actgaccagc
     7381 ggtgagcgcg cgatccagca cggtcaggcg attgccgccg ggataaaagc agcgaaagat
     7441 ctcggtaata tgccgccgaa tatctgtaac gccgcttatc tcgcctctca ggcgcgccag
     7501 ctggctgaca gctacagcaa gaatgtcatc acccgcgtta tcggcgaaca gcagatgaaa
     7561 gagctgggaa tgcactctta tctggcggtc ggtcagggtt cacaaaacga atcgctgatg
     7621 tcggtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt gctggtgggt
     7681 aaaggtttaa ccttcgactc cggcggtatc tccatcaagc cttcagaagg catggatgag
     7741 atgaagtacg atatgtgcgg cgcggcggca gtttacggcg tgatgcgtat ggtcgcggag
     7801 ctgcaactgc cgattaacgt tattggcgtg ctggcaggct gtgaaaacat gcctggcggg
     7861 cgtgcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaaccgt tgaagtgctg
     7921 aacaccgatg ccgaaggccg cctggtactg tgcgacgtgt taacttacgt tgaacgtttt
     7981 gagccggaag cagtgattga cgtggcgacg ctgaccggtg cctgcgtgat cgcgctgggt
     8041 catcatatta ctggtctgat gacgaaccat aatccgctgg cccatgaact gattgccgcg
     8101 tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg gtgacgagta tcaggaacag
     8161 ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg ggcgattacc
     8221 gcaggttgct tcctgtcacg ctttacccgt aagtataact gggcgcacct ggatattgca
     8281 ggaaccgcct ggcgttctgg taaagcaaaa ggcgcaaccg gtcgtccggt agcgttgctg
     8341 gcacagttcc tgttaaaccg cgctgggttt aacggcgaag agtaagtttg cgttttaccc
     8401 tcaccctaac cctctcccta agggagaggg aaccgatcga gcgcaataga aattcattgc
     8461 tcatctttca gggtgaggaa tgcagcaata tttaaatcca ccacaagaag ccccatatat
     8521 gaaaaacgca acgttctacc ttctggacaa tgacaccacc gtcgatggct taagcgccgt
     8581 tgagcaactg gtgtgtgaaa ttgccgcaga acgttggcgc agcggtaagc gcgtactcat
     8641 cgcctgtgaa gatgaaaagc aggcttaccg gctggatgaa gccctgtggg cgcgtccggc
     8701 agaaagcttt gttccgcata atttagcggg agaaggaccg cgcggcagtg cgccggtgga
     8761 gatcgcctgg ccgcaaaaac gcagcagcag cccgcgcgat atattgatta gtctgcgaac
     8821 aagctttgca gattttgcca ccgctttcac agaagtggta gacttcgttc cttatgaaga
     8881 ttctctgaaa caactggcgc gcgaacgcta taaagcctac cgcgtggctg gtttcaacct
     8941 gaatacggca acctggaaat aatggaaaag acatacaacc cacaagatat cgaacagccg
     9001 ctttacgagc actgggaaaa gcagggctac tttaagccta atggcgatga aagccaggaa
     9061 agtttctgca tcatgatccc gccgccgaac gtcaccggca gtttgcatat gggtcacgcc
     9121 ttccagcaaa ccatcatgga taccatgatc cgctatcagc gcatgcaggg caaaaacacc
     9181 ctgtggcagg tcggtactga ccacgccggg atcgctaccc agatggtcgt tgagcgcaag
     9241 attgccgcag aagaaggtaa aacccgtcac gactacggcc gcgaagcttt catcgacaaa
     9301 atctgggaat ggaaagcgga atctggcggc accattaccc gtcagatgcg ccgtctcggc
     9361 aactccgtcg actgggagcg tgaacgcttc accatggacg aaggcctgtc caatgcggtg
     9421 aaagaagttt tcgttcgtct gtataaagaa gacctgattt accgtggcaa gcgcctggta
     9481 aactgggacc cgaaactgcg taccgctatt tctgacctgg aagtggaaaa ccgcgaatcg
     9541 aaaggttcga tgtggcacat ccgctatccg ctggctgacg gcgcgaaaac cgcagacggt
     9601 aaagattatc tggtggttgc gaccactcgc ccggaaaccc tgctgggcga taccggtgta
     9661 gctgttaacc cggaagatcc gcgttacaaa gatctgattg gcaaatatgt cattctgccg
     9721 ctggttaacc gtcgtattcc gatcgttggc gacgaacacg ccgacatgga aaaaggcacc
     9781 ggctgcgtga agatcacacc ggcgcacgac tttaacgact atgaagtcgg taaacgtcac
     9841 gccctgccga tgatcaacat cctgaccttt gacggtgata tccgtgaaag cgcccaggtg
     9901 ttcgatacca aaggtaacga atctgacgtt tattccagcg agatcccggc agagttccag
     9961 aaactcgagc gttttgctgc acgtaaagca gtcgttgctg cggttgatgc actcggcctg
    10021 ctggaagaaa ttaaaccgca cgacctgacc gttccttacg gcgaccgtgg cggcgtggtt
    10081 atcgaaccga tgctgaccga ccagtggtac gtgcgtgccg atgtactggc gaaaccggca
    10141 gttgaagcgg ttgagaacgg cgacattcag ttcgtaccga agcagtacga aaacatgtac
    10201 ttctcctgga tgcgcgatat tcaggactgg tgtatctctc gtcagctgtg gtggggtcac
    10261 cgtatcccgg catggtatga cgaagcgggt aatgtttatg ttggccgcaa cgaagaagaa
    10321 gtgcgcaagg aaaataacct cggtgccaac gttgccctgc gtcaggacga agacgttctc
    10381 gacacctggt tctcttctgc actgtggacc ttctctaccc ttggctggcc ggaaaatacc
    10441 gacgccctgc gtcagttcca cccaaccagc gtgatggtat ctggtttcga catcatcttc
    10501 ttctggattg cccgcatgat catgatgacc atgcacttca tcaaagatga aaatggcaaa
    10561 ccgcaggtgc cgttccacac tgtttacatg accggtctga ttcgtgacga cgaaggtcag
    10621 aagatgtcca aatccaaggg taacgttatt gacccgctgg atatggttga cggtatttcg
    10681 ctgccagaac tgctggaaaa acgcaccggc aatatgatgc agccgcagct ggcggacaaa
    10741 atccgtaagc gcaccgagaa acagttcccg aacggcattg agccgcacgg caccgatgcc
    10801 ctgcgcttca ccctggcggc gctggcgtct accggtcgtg acatcaactg ggatatgaag
    10861 cgtctggaag gctaccgtaa cttctgtaac aagctgtgga acgccagccg ctttgtgctg
    10921 atgaacacag aaggtcagga ttgcggcttc aacggcggcg aaatgacgct atcgctggcg
    10981 gaccgctgga ttctggcgga gttcaaccag actatcaaag cgtaccgcga agcgctggac
    11041 agcttccgct tcgatatcgc cgcaggcatt ttgtatgagt tcacctggaa ccagttctgc
    11101 gactggtatc tggagctgac caagccggta atgaacggtg gaacagaagc cgaactgcgc
    11161 ggtactcgcc atacgctggt gactgtactg gaaggtctgc tgcgcctcgc gcatccgatc
    11221 attccgttca tcaccgaaac catctggcag cgtgtgaaag tactttgcgg tatcactgcc
    11281 gacaccatca tgctgcagcc gttcccgcag tatgatgcat ctcaggttga tgaagccgca
    11341 ctggccgaca ccgagtggct gaaacaggcg atcgttgctg tacgtaacat ccgtgcagaa
    11401 atgaacatcg cgccgggcaa accgctggag ctgctgctgc gtggttgcag cgcggatgca
    11461 gaacgtcgcg taaatgaaaa ccgtggcttc ctgcaaacgc tggcgcgtct ggaaagcatc
    11521 accgtgctgc ctgccgatga caaaggtccg gtttccgtta ccaagatcat cgacggcgca
    11581 gagctgctga tcccgatggc tggcctcatc aacaaagaag atgagctggc gcgcctggcg
    11641 aaagaagtgg cgaagatcga aggtgaaatc agccgcatcg agaacaagct ggcaaatgaa
    11701 ggctttgtcg cccgcgcacc ggaagcggtt atcgcgaaag agcgtgagaa gctggaaggt
    11761 tatgcagaag cgaaagccaa gctgattgaa cagcaggctg ttatcgccgc actgtaatca
    11821 acacagtgat gaaaacgaag gccggagcat gctccggcct tttttatctc ttacagaaat
    11881 ggtaaagaag gcataactcc ccgagaaata ctggctaaga agcctttatc tggttgttct
    11941 tctttattga ttaaaggaag gtcatccaaa tcgcccagca aatacgtatt ttttgcctgc
    12001 cagtcaatca tccatatttt cagcagtggg taagccagac cacccgtaat cccggatatc
    12061 accaccaacg cacacatgcg ataaagcata ccgtggtacg ttaaagttga gcgaaaacga
    12121 atacgcccat cattcagtga caggttgctc ataaaatgat ttcgcaacga caccgttaaa
    12181 taactggtgc taacagcaat cccaaaataa taaattaact gcgcgattat cattttacgt
    12241 tgcatttcca taaattgctg taaattctca atatcatcat ttgcataccc agaactatca
    12301 tactcattta atatttgatc gaagataata taaccagcaa cagcgagaaa aggtaataaa
    12361 gctaaaaatg cgaggatggc atattttata caatacgcag tatctaattt caccttgaaa
    12421 cgatgtatac cgaaactggt attgctccag agaaaactca ttaccaggct atataaagta
    12481 ccattaaaaa taccaatgga aacaatacca actattgcca tcagaaccac agatataata
    12541 acactacttg aactattggc atgtagcatc tttgtcgaga taaagaaaac agtccccatc
    12601 ccaatggcca ttaaaatcgg caagaaaaag gttacccacc agaacccttt caaagagcaa
    12661 ttaaaactaa atcttacgcc gttgagactg gtcattaagg cctgataacg cagtcctttt
    12721 gctgccataa atataagcaa aaccaacagt aacaaagtca aaacacagcc aacaagcggc
    12781 atatctgctg acactgtcat taagattgcg aaatagaaaa aaacaaaaac aagacaacta
    12841 acaaaaacat tcccaccggt aattccataa gaaaatcgtt gtccgttaac ttccatatta
    12901 gcataaagat aacgcttaca tttcattaat gcccatggta aatagatacc tagcgttata
    12961 atcgttaaca acacattcac cacacaaata agaaaatatc tctcacctgt accatgaaaa
    13021 acaaaagaat gggacggaac atccatttca ttaataactt gagccataaa aatcccctga
    13081 attaaaaaat ccattgatat aatttataga tacagacagt aaaatgttct gccacgttac
    13141 aatactctta tcagtaatag gctggcaaaa actttat
//
LOCUS       DAZPAB010000103        13017 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZPAB010000103 DAZPAB010000000
VERSION     DAZPAB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13017
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.103"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..319)
                     /locus_tag="ACHUPG_002614"
     CDS             complement(<1..319)
                     /locus_tag="ACHUPG_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFR0862175.1"
                     /translation="MAISDTKLRTIYGKPYSGPQELADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFD"
     gene            complement(525..1244)
                     /locus_tag="ACHUPG_002615"
                     /pseudo
     CDS             complement(525..1244)
                     /locus_tag="ACHUPG_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1260..1592)
                     /gene="cusF"
                     /locus_tag="ACHUPG_002616"
     CDS             complement(1260..1592)
                     /gene="cusF"
                     /locus_tag="ACHUPG_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR0862176.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1616..2998)
                     /gene="cusC"
                     /locus_tag="ACHUPG_002617"
     CDS             complement(1616..2998)
                     /gene="cusC"
                     /locus_tag="ACHUPG_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR0862177.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            3155..3838
                     /gene="cusR"
                     /locus_tag="ACHUPG_002618"
     CDS             3155..3838
                     /gene="cusR"
                     /locus_tag="ACHUPG_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR0862178.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3828..5270
                     /gene="cusS"
                     /locus_tag="ACHUPG_002619"
     CDS             3828..5270
                     /gene="cusS"
                     /locus_tag="ACHUPG_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR0862179.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5420..7657
                     /gene="nrfB"
                     /locus_tag="ACHUPG_002620"
     CDS             5420..7657
                     /gene="nrfB"
                     /locus_tag="ACHUPG_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFR0862180.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7644..10612
                     /gene="nfrA"
                     /locus_tag="ACHUPG_002621"
                     /pseudo
     CDS             7644..10612
                     /gene="nfrA"
                     /locus_tag="ACHUPG_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10613..11503
                     /locus_tag="ACHUPG_002622"
     CDS             10613..11503
                     /locus_tag="ACHUPG_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR0862181.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11686..12447
                     /gene="envY"
                     /locus_tag="ACHUPG_002623"
     CDS             11686..12447
                     /gene="envY"
                     /locus_tag="ACHUPG_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR0862182.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12493..12569)
                     /locus_tag="ACHUPG_002624"
     tRNA            complement(12493..12569)
                     /locus_tag="ACHUPG_002624"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12533..12535),aa:Arg,seq:tct)
     gene            12812..>13017
                     /locus_tag="ACHUPG_002625"
     CDS             12812..>13017
                     /locus_tag="ACHUPG_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFR0862183.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3188 a         3376 c         3304 g         3149 t
ORIGIN      
        1 tgtcgaacca gtagtcgaga cactgggcga ccgtcatgga gttctccacc ttctcttcaa
       61 aataagtgcg tggatccgtt cctgagaaat agagctttcg caagtcagca gtgatctgtc
      121 tggcatcctt caaagacagg gatgggtatc gaccaagccc aagtcgatta ggcttgccat
      181 gccagcgata gcggtactgg aactggatga cccccttcgg tgaaattcgt acgctgaggc
      241 catcagcatc agccaattct tgtgggccag aatatggttt accataaata gtacgcagtt
      301 ttgtgtcgct tatagccata aataatattt ggtacgctca gaaataatgg tttggtacac
      361 atctggtaca caatatcaca tggacaacag cgcacagtaa acaactatat ttgacgttcg
      421 tagacatact caggtgaaaa aaaggttgtt tttcgagtta taacagacag ttaattaact
      481 acagtggaca atgctagaca atgacagaca cagataagca acctaccttc ctctttcgcg
      541 accacgttat ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca
      601 tcaatgggcg ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggtggcga
      661 atatccgcct ccggcattcc cgccagtcgc agccgctcaa gaatgccttc agtctgggtc
      721 gccgtaccgc cggtttcgcg caacagtaaa tactcactct gcgcttccac ccagtcggga
      781 atggtcaggt cgagaagcgg tgtgcccttt tgtactttat cgcccacggt aagcggatac
      841 accttgtcga taaacccggc agcgcgagcc tgcacaatgg catactgata ctcgttgtaa
      901 ctgacattcg tcgggaaact ctgggcaaaa gtcagcggcc cgcgcgtgac ggtagccgtt
      961 ttcaccccca gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct
     1021 tcatcggcat atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg
     1081 aaccgcgtat tgggatacat cgggtcgtac cagaataaga ttttacgttc tgcggtcggc
     1141 attttttctg cgtgcggttc cgcctggtca aaccaggtaa aacctgccgc tgaaataata
     1201 ccgcccgcga tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt
     1261 tactggctga ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac
     1321 gccactttgt cgccggtttt aatttcactc attttcgtct gcggggtgat ggtaaagcgc
     1381 atggtcatct ccggccagtt gacggcagca atcggatcgt gatggatggt gatttttttg
     1441 ctttccagat caacaccttt taccacaccg gtggcactaa tgacctgtgg ttgtgcttcg
     1501 ctcatggttt catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga
     1561 ctgaacattg cgacttgcag tgcttttttc atttattctc tcctggagtt aaaaattatt
     1621 gctgccaacc gccccctaag gcggtataca aataaatttc gttaacctga cgggcataat
     1681 tcaggtcaag taaagtttgt cgggttgcaa ataaagaacg ctcggcatcc agcacttcca
     1741 gataacttac cgcgccgtgc tgatataatg cccgcgcccg ttgcagggta atttgcagcg
     1801 acgccagata acgctgctgg gcgctgattt gatcgtccag gctttgacgt aatgccagcg
     1861 catctgccac ctctttaaag gcgttctgga ttttctgttc ataattcacc accgactgct
     1921 gctggcgaat ttcggcgata tccagattgg cctggttgcg tccggcatta aaaatgggga
     1981 tctcaatttt aggaataaaa ttccacatcc cgctgctagc gttaaataat gacgacagat
     2041 cgctgctggc ggttgatatt ccgctggtca ggctgataga cgggaaaaat gccgcacgcg
     2101 cagcaccaat attagcatta gccgccatta acgcgtgttc agcttccata atatccgggc
     2161 gctgcaataa gatttgcgac gacaagcccg ccggtaattt aacgctttgc aggctgccgc
     2221 tgtttactgt ctgcgcttgc ggtagcttgc cgtagcttcc caataacagt tgcaatgcat
     2281 tattcgcctg cgccagttcc ccctgacgtt tagcgatgtc actgcgggta ctttctatca
     2341 ccccgcgagc ctgttccagc gccagaacat tgctgctacc ggtcaacagt tgtttttcga
     2401 caaacgcata tgactgctga taattacgca gcgtttcttc ggctatttgc aattgcgcat
     2461 acgccagctg ctgattgaaa tagctttgcg cgacattaga aaccagcaaa atatgcaccg
     2521 cgcgctgggc ttcctcagtg gctaaataat tttggcgctc ggcttcgctc atgttcttta
     2581 agcgaccgaa aaaatcgaga tcaaagctgg cgttaaggcc agtcgagaac tcccgcgtcg
     2641 tggctgaatc gcctttaaga ttgccgctcc agctgccgct gccctcgcca ttgagctgtg
     2701 ggtagcggtc ggcatcggtc agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg
     2761 ccatgcgcaa atcccggtta ttcaccagcg cctcgctaat cagcgtcttc acctgattat
     2821 caacaaaaaa ggtgcgccag cccacgttct ggtagttatc cgctgcgtta accaggccgt
     2881 tctggctgag tgagaactgc tgcggtacgg gcattaccgg acgctgataa tccggtgcca
     2941 gtgaacaacc agttagcgca agggccacac aaaatggcag aagtttacaa ggagacatag
     3001 gctcataatt tctggtgatt ttatgtcgcc aactttactc gccaggctct gattttccgg
     3061 tgacaggaaa atgacaaaat tgtcattttg ccaataagcg attgccatct gatcccgcta
     3121 ctctagaatt gcccgggcaa catgcggagg aaatatgaaa ctgttgattg tcgaagatga
     3181 aaagaaaacc ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt
     3241 ggccgacaac gggctgaatg gctaccatct ggcgatgacc ggtgattatg acctgataat
     3301 cctcgatatt atgctgccag acgtgaacgg ctgggatatc gtgcgcatgt tgcgctccgc
     3361 caataaaggg atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa
     3421 ggggctggag ttgggggcag atgactacct ggtgaaaccg ttcgcttttg ctgaactgct
     3481 ggcgcgggtg cgcactctcc tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca
     3541 ggttgccgat ctgatggtcg atctcgtcag ccgcaaagtc acccgcagcg gcacgcgcat
     3601 cactctgacc agtaaagaat ttactctgct ggagtttttt ctccgccatc agggcgaagt
     3661 gctgccccgc tcgcttatcg cctcgcaggt atgggacatg aatttcgaca gcgacactaa
     3721 cgccattgat gtggcggtga agctgctacg cggcaaaatc gacaacgact ttgagccgaa
     3781 gctgattcaa accgtgcgcg gcgtgggtta catgcttgag gtgccggatg gtcagtaagc
     3841 catttcagcg cccgttttcg ctggcaaccc gcctgacctt ttttatcagc caggccacca
     3901 tcgcggcgtt tttcgccttt gcatggatca taatccactc agtaaaagtg cattttgccg
     3961 agcaggacat taatgattta aaagagatta gcgccaccct tgaacgggta ctaaatcacc
     4021 ctgacgaaac gcaagcccga cgcttaatga cgctggaaga tatcgtcagt ggttattcca
     4081 acgtgttgat ttccctggca gatagccacg gcaaaacggt gtatcactcc cccggtgcgc
     4141 ctgatatccg cgagtttgca cgtgatgcca tacccgataa agacgcgcgg ggtggcgagg
     4201 tgtatctcct ttccggcccg acgattatga tgccaggcca cggtcacggg catatggaac
     4261 acagcaactg gcggatgatt aacttgccgg ttggcccgtt ggtggacggc aaaccgattt
     4321 atacgctcta catcgcgctg tcgatcgatt ttcatcttca ttacataaat gatttgatga
     4381 ataaacttat tatgaccgca tcggtaatca gcatcctgat cgtctttatc gtgctgttgg
     4441 cggtacataa aggtcacgcg ccgatccgca gcgtcagccg tcaaatccag aatatcacct
     4501 cgaaagatct cgacgttcgc cttgacccgc agaccgtgcc gattgagctg gaacagctgg
     4561 tactgtcgtt caaccatatg atcgagcgta ttgaggatgt ctttactcgc cagtccaatt
     4621 tctcagcgga tatcgcccac gaaattcgca caccaattac taatctcatt acgcaaacgg
     4681 aaatcgccct cagccagtcg cgcagccaga aggagctgga agatgtgctc tactctaatc
     4741 tcgaagagct gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc gctcaggccg
     4801 ataacaacca gctaatcccc gaaaagaaaa tgctcaacct ggcggatgaa gtcggcaaag
     4861 tgttcgattt tttcgaggcg ctagcggaag atcgcggcgt ggagttgcgg tttgttggcg
     4921 acaaacgtca ggttgcgggc gatccgttga tgctgcgtcg ggcgctaagc aacctgcttt
     4981 ctaatgccct tcgttatacg ccaaccagag agacaattgt agtacgctgt cagacggtcg
     5041 atcaccaggt gcaagtttcc gtcgaaaacc ccggtacgcc cattgcgccc gagcacttac
     5101 cgcgattgtt tgaccgtttc tatcgcgttg acccctcccg ccagcgaaaa ggtgaaggta
     5161 gcggtattgg gctggcgata gtgaaatcga ttgttgtcgc gcataaaggc acggttgcgg
     5221 taacgtcaga tgcgcggggg acaaggtttg ttatcacatt acccgcttaa ttattcagca
     5281 aacggcaact tttataacca gtgtaaaaat aacgtgccgc aatagtcata catattaatg
     5341 gtaaaaagct gtcacaattc ataaaaaacc ttaatatacg ccaccctaaa cataaccagc
     5401 gttaatgtaa ggtttttgtg tggactggct tcttgatgtt tttgctacct ggctctacgg
     5461 cttaaaagta atcgcgataa cgttagcggt catcatgttc atcagcgggc tggacgattt
     5521 ttttattgat gtcgtctact gggtacgccg cattaaacgc aagttgagtg tttatcgccg
     5581 ctacccgcga atgagttacc gcgaactgta taaaccagat gaaaaaccgt tagcgattat
     5641 ggttccggcg tggaatgaaa cgggcgtcat cggcaatatg gccgagctgg cggcgaccac
     5701 gctcgactac gaaaactatc atatctttgt tggcacctac cccaacgacc ccgatactca
     5761 acgtgatgtt gacgaagtgt gcgctcgctt tccgaacgtg cataaggtag tctgcgcgcg
     5821 tcctggtccc accagcaaag ccgactgcct gaacaacgtg ctggacgcca tcacccagtt
     5881 tgaacgtagc gccaatttcg cttttgctgg ttttattctg catgacgccg aagatgtgat
     5941 ttcaccgatg gaattgcgtc tgttcaacta tctggtcgag cgtaaagatc tgattcagat
     6001 cccagtgtat ccgtttgaac gcgaatggac gcacttcacc agcatgactt acattgatga
     6061 gttttcagaa ctgcatggca aagatgttcc ggtgcgtgaa gccctcgccg gacaagtgcc
     6121 cagcgcaggc gtcggcacct gtttcagccg ccgcgccgtg accgcactgt tagctgacgg
     6181 taacggtatt gctttcgacg tgcagagtct tactgaagat tacgacattg gcttccgcct
     6241 gaaagaaaaa ggtatgacgg aaatttttgt ccgttttccg gtggtggacg aagccaaaga
     6301 acgcgagcag cgtaaatttt tacagcacgc acggacgtca aacatgatct gcgtgcgcga
     6361 atatttcccc gatacctttt cgactgcggt tcgacaaaaa tctcgctgga tcatcggcat
     6421 tgttttccag ggctttaaaa cccataaatg gacctccagc ctgacgctga actactttct
     6481 ctggcgcgac cgcaaagggg caatcagtaa ctttgtcagc ttcctcgcga tgctggtgat
     6541 gatccagctt ttgctgttgc tggcgtatga aagtttgtgg cccgatgcct ggcatttcct
     6601 ttctattttt agcggcagcg catggttaat gaccctgctg tggctaaact ttggcttgat
     6661 ggttaaccgc atcgtgcagc gagtgatttt cgtcactggc tactacggcc tcacgcaggg
     6721 cctgctttcc gtcctgcgtc ttttctgggg caacctgatt aactttatgg ctaactggcg
     6781 cgtgctgaaa caggttcttc agcacggcga tccgcgtcgc gtcgcgtggg ataaaacaac
     6841 gcatgacttc cccagcgtca ctggcgatac ccgctcgttg cgcccgttag gtcaaattct
     6901 gctggaaaat caggtcatca ctgaagaaca actcgataca gcactgcgta atcgcgtcga
     6961 aggtctacgc ctgggcggtt caatgctgat gcaggggctg attagcgccg agcagctggc
     7021 acaggcgctg gcagagcaaa acggcgtggc gtgggaatcc atcgatgcct ggcagatccc
     7081 gtcctcgctg attgccgaaa tgccagcctc cgtagcgctg cattatgcag tactgccgct
     7141 gcgtctggaa aatgatgagt taattgtcgg cagtgaagat ggtattgacc cggtttcgct
     7201 ggcggccctg acgcgtaaag tcggacgcaa agtgcgttac gtcattgttc tgcggggaca
     7261 aattgtcaca gggttacgcc actggtatgc acgccgacgc ggtcacgatc cgcgggcaat
     7321 gttgtacaat gcggttcagc atcagtggct cacggaacag cagaccggtg aaatctggcg
     7381 gcaatatgtg ccgcatcagt tcctgttcgc cgaaatactg accacgctcg gtcatattaa
     7441 tcgttcagca attaacgtgt tgttattgcg ccatgaacgc agttctctgc cgctcggcaa
     7501 gtttttggtc accgaaggcg ttatcagcca ggaaacgttg gatcgcgtcc tgacaattca
     7561 acgcgaatta caagtttcga tgcaatcact attactcaaa gcaggtttaa acacagaaca
     7621 ggttgcgcaa ctggagtccg aaaatgaagg agaataacct taatcgcgtc atcggatggt
     7681 ctggtttact gctgacgtct ttattgagta ccagcgcact cgcagacaat atcggcacca
     7741 gcgcagaaga gctggggctg agcgattatc gccattttgt tatttatccc cgtctcgata
     7801 aggcgctgaa ggcacagaaa aataacgacg aagcaaccgc catccgcgaa tttgaatata
     7861 tacaccaaca ggtgccggat aatattccgc tgactttata ccttgcggaa gcctatcgtc
     7921 attttggtca tgatgaccgg gcgcggctgt tgcttgagga tcaactgaaa cgtcacccag
     7981 gagatgcccg acttgagcgc agtctggcgg ctattccagt tgaagtgaaa agcgttacga
     8041 ctgttgaaga actgcttgcc cagcaaaaag cgggcgacgc tgcgccgacc ctgcgttgtc
     8101 gcagtgaagt cgggcagaat gccctgcggc tggcacaatt acctgtcgcc agagcgcaac
     8161 tgaacgatgc gacgtttgct gcatcgccgg aaggaaaaac gctgcgaacc gatctgctgc
     8221 aacgggcaat ctacctgaaa caatgatccc aggcagatac gctatacaat gaagcacgcc
     8281 agcagaacac attaagcgcg gcagaacacc gtcagtggtt tgacgtgctt cttgccgggc
     8341 agctggacga tcggatcctg gcactgcaat cacaggggat cttcaccgat cctcagtcat
     8401 atattactta cgcgaccgcg ctggcttatc gtggcgaaaa agcacgcctc cagcattatc
     8461 tcattgaaaa taagccgctg tttaccacgg gcgcacaaga gaaaagttgg ctctatctgt
     8521 tatctaaata cagcgccaac cccgttcagg cattggcgaa ttatacggtg cagtttgccg
     8581 ataaccgcca gtatgttgtt ggcgcgacgc taccggtgct gttaaaagaa ggtcagtacg
     8641 acgcagcgca aaaactgctc gccaccctcc ccgccaatga aatgcttgag gagcgttatg
     8701 ctgtcagcgt ggcgacccgt aacaaggctg aatctctgcg tctggcacga ttgctgtatc
     8761 agcaagaacc gacaaatctt ccccgcctgg atcaactgac ctggcaactg atgcagaacg
     8821 agcagtcacg cgaagctgcc gatttattgc tgcaacgcta tcctttccag ggcgatgcgc
     8881 gtgtcagcca gactttaatg gcgcgactgg cgtctctgct ggaaagtcat ccttacctag
     8941 caacgccggc gaaggtggcg attttatcga aacccttacc gctggcggag caacgtcagt
     9001 ggcaaagtca gttgccgggt attgcagata attgcccggc aatagttcgc ttgctgggcg
     9061 atatgtcgcc ttcctacgat gctgccgcct ggaaccgtct ggcaaagtgt tatcgggaca
     9121 cgctacccgg tgtggcgttg tatgcatggc ttcaggccga acaacgacaa ccgagcgcct
     9181 ggcaacatcg tgcggtagcc tatcaggcgt atcaggttga ggactacgcc accgcactgg
     9241 cggcctggca gaaaatcagt cttcacgaca tgagcaatga tgatctgctc gccgccgcca
     9301 acaccgctca ggcagcggga aatggcgcag ctcgcgatcg ctggcttcaa caggcagaac
     9361 aacgtgagct gggaaacaat gctcaatact ggtggctgca tgcgcgacgt tatatcccag
     9421 ctcagccaga actcgcactg aacgatctca cgcgctcaat caatattgcc ccttctgcca
     9481 actcttacgt tgcgcgggcg acaatttatc gccaacgtca taatgttccg gccgcggtga
     9541 gtgatttgcg cgccgcgctg gaactggaac caaataatag caacacccag gcagcgcttg
     9601 gttacgcctt gtgggatagc ggtgatatcg cacagtcgcg ggaaatgctc gaacaggcgc
     9661 ataaagggct tccggacgat ccggcactga tccgacaact ggcctacgtg aaccagcgtc
     9721 tggatgacat gcctgcgacg cagcactacg cccggctcgt gattgatgac attgataatc
     9781 aggcataacc ccactgaccc cagagcaaaa tcagcaacgc ttcaatttcc gccgtttgca
     9841 tgaggaggtc ggtcgccgct ggacgttcag tttcgattct tccatcggct tgcgttccgg
     9901 cgcaatgagt accgctaaca ataatgtcgg cggtgcagcg cctgggaaaa gctatcgtag
     9961 ctacggacaa ctggaagccg agtaccgcat cggacgcaat atgctgctgg aaggcgacct
    10021 gctctcagtt tatagccgcg tctttgccga taccggagaa aacggggtga tgatgccggt
    10081 gaaaaatccg atgtccggca ccggtctgcg ctggaagccg ctgcgcgatc agatcttttt
    10141 cctcgccgtc gaacagcagt tgccgctgaa cgggcaaaat ggcgcatccg ataccatgct
    10201 gcgcgccagc gcctcattct ttaatggcgg caaatacagc gacgaatggc acccgaacgg
    10261 ttcaggctgg tttgcccaaa acctgtacct cgatgcggcg caatatatcc gccaggatat
    10321 tcaggcgtgg acggcagatt atcgcgtcag ctggcatcag aaggtagcta acggacagac
    10381 tattgagcct tacgctcacg ttcaggacaa cggctatcgt gataaaggca ctcagggcgc
    10441 gcagcttggc ggtgtcgggg tccgctggaa tatttggacc ggcgagacgc actacgacgc
    10501 ctggccgcac aaagtcagtc tcggcgtcga gtatcaacat acctttaagg cgattaatca
    10561 acgtaacgga gagcgcaaca acgcgtttct caccattgga gtgcactggt aaatgcgtaa
    10621 gttcattttc gtattgctga cactgctttt ggtcagccct ttttcctttg cgatgaaagg
    10681 tattatctgg caaccacaaa accgagatag tcaggttacc gatacccagt ggcaggggct
    10741 gatgagtcag ttacgttttc aaggcttcga taccctggtt ttgcaatgga cccgttacgg
    10801 cgatgcattt acccagccag aacagcgcac gttattgttt aagcgtgccg cagctgcgca
    10861 acagactggc ctgaagctga ttgtcgggct gaacgccgat ccggaatttt ttatgcacca
    10921 gaaacagtcg tctgcagcgc tggaaagcta tcttaatcgc ctgctggctg ccgatctcca
    10981 gcaagccaga ttatggagcg ccgcgcctgg cataacgccg gatggctggt acatcagcgc
    11041 ggaaattgac gacctgaact ggcgcagcga agccgcccgt caacctttgc taacatggtt
    11101 aaacaacgcg cagcggctga ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt
    11161 tttcgccgga aacatgtcgc ccgatggcta tcgccaactg ctggaacaag ttaaagcaac
    11221 cggagttaat gtctgggtac aggatggcag cggcgtggat aaacttaccg ctgaacagcg
    11281 tgaacgttat ctacaggcca gcgccgattg ccaaagctcc gccccagcaa gcggcattgt
    11341 ttatgaactg tttgtcgccg gcaaaggcaa aacctttaca gcgaaaccga aaccggacgc
    11401 agaaattgcc tcgctgttag cgaaacgttc ctcttgcggt aaagacatgc tctatttctc
    11461 tctgcgctat ttgcccgtcg cgcacggcat tctcgagtat taaatcccct ccaggtaagt
    11521 cgggtacgac ctggcttacc tctttcgctc tcgtcagaat tccatcaaga ttaattgcta
    11581 aaaagcatgc taaagaaaga agatcgttat cggcgaacgg gccgatcact catgcaggaa
    11641 gcatagatcc tttgagcgga gtttctgata tctgaggagt gcgaaatgca attgagcagc
    11701 agtgaacctt gcgtggtgat cctgaccgaa aaagaggtag aggtaagcgt caataaccat
    11761 gctacgttta cccttccgaa aaactacctg gccgccttcg cgtgcaacaa taacgtcatt
    11821 gaactctcaa cgttaaatca cgtattaatc acccacatca accgtaacat catcaacgat
    11881 tatctgttgt ttttaaataa gaaattaacc tgtgtaaagc cctggtcgcg gctagcaacc
    11941 ccggttatcg cttgtcatag ccgtacaccg gaagtgttcc ggctagccac caaccacagc
    12001 aagcagcaat ccagcaaacc ctgcgaagcg gagttgacgc gcgcattact ttttaccgta
    12061 ttgtctaact ttcttgagca atcgcggttt attgccctac tgatgtatat cttacgcagc
    12121 agcgtccgcg acagcgtctg ccgcattatt caaagcgata ttcagcatta ctggaatctg
    12181 cgaattgtcg ccagttcgct atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac
    12241 gaaaatacca gctatagcca gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg
    12301 ttattgatgg ataacaaaaa tatcactcag gtggcgcaat tatgtggcta tagcagcacg
    12361 tcgtacttta tctctgtttt taaggcgttt tacagcctga caccgttgaa ttatctcgcc
    12421 aaacagcgac aaaaagtgat gtggtgaagg gcaaagcgga aacggataag acgggcataa
    12481 atgaagaaga aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc
    12541 gttgctctat ccaactgagc taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga
    12601 attatacggt caacccttgc tgagtcaatg gcttttgatc tggttgctga acaagtgaac
    12661 gaccgcgtct gattttctga tttatttcgc tatagcggca aacaaacgca caccgctgcg
    12721 cgtctgaatc aagaaaaccc gtattttcat gtatcaaagt acaatttacc gccctaacgg
    12781 aaaattgtcc gctcctatga gactggtaac tatgaaacca acgtcggtga tcattatgga
    12841 tactcatcct atcatcagaa tgtctattga agttctgttg caaaaaaaca gtgaattgca
    12901 gattgtcctg aaaacggatg attaccgcat aaccatcgat tatctccgaa cccgtcctgt
    12961 tgatttaatc attatggata tagacttgcc cggaacagac ggttttacct tcctgaa
//
LOCUS       DAZPAB010000104        12908 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZPAB010000104 DAZPAB010000000
VERSION     DAZPAB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.104"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="ACHUPG_002626"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="ACHUPG_002626"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFR0862184.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="ACHUPG_002627"
     CDS             complement(1806..3224)
                     /locus_tag="ACHUPG_002627"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR0862185.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="ACHUPG_002628"
     CDS             complement(3378..3722)
                     /locus_tag="ACHUPG_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFR0862186.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="ACHUPG_002629"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="ACHUPG_002629"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR0862187.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5653)
                     /locus_tag="ACHUPG_002630"
                     /pseudo
     CDS             complement(4712..5653)
                     /locus_tag="ACHUPG_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="ACHUPG_002631"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="ACHUPG_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR0862188.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="ACHUPG_002632"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="ACHUPG_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR0862189.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="ACHUPG_002633"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="ACHUPG_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR0862190.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="ACHUPG_002634"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="ACHUPG_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR0862191.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="ACHUPG_002635"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="ACHUPG_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR0862192.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="ACHUPG_002636"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="ACHUPG_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR0862193.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="ACHUPG_002637"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="ACHUPG_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFR0862194.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11368..12483
                     /gene="ydeE"
                     /locus_tag="ACHUPG_002638"
                     /pseudo
     CDS             11368..12483
                     /gene="ydeE"
                     /locus_tag="ACHUPG_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="ACHUPG_002639"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="ACHUPG_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFR0862195.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            12705..12808
                     /gene="mgtT"
                     /locus_tag="ACHUPG_002640"
                     /pseudo
     CDS             12705..12808
                     /gene="mgtT"
                     /locus_tag="ACHUPG_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
BASE COUNT         3401 a         3299 c         2950 g         3258 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc caaggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagagcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgagaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    12901 ctcaatga
//
LOCUS       DAZPAB010000105        12476 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZPAB010000105 DAZPAB010000000
VERSION     DAZPAB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12476
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.105"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            42..1556
                     /gene="caiT"
                     /locus_tag="ACHUPG_002641"
     CDS             42..1556
                     /gene="caiT"
                     /locus_tag="ACHUPG_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFR0862196.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1587..2729
                     /gene="caiA"
                     /locus_tag="ACHUPG_002642"
     CDS             1587..2729
                     /gene="caiA"
                     /locus_tag="ACHUPG_002642"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR0862197.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2858..4075
                     /gene="caiB"
                     /locus_tag="ACHUPG_002643"
     CDS             2858..4075
                     /gene="caiB"
                     /locus_tag="ACHUPG_002643"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFR0862198.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4149..5702
                     /gene="caiC"
                     /locus_tag="ACHUPG_002644"
     CDS             4149..5702
                     /gene="caiC"
                     /locus_tag="ACHUPG_002644"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR0862199.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5811..6596
                     /gene="caiD"
                     /locus_tag="ACHUPG_002645"
     CDS             5811..6596
                     /gene="caiD"
                     /locus_tag="ACHUPG_002645"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR0862200.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6602..7192
                     /gene="caiE"
                     /locus_tag="ACHUPG_002646"
     CDS             6602..7192
                     /gene="caiE"
                     /locus_tag="ACHUPG_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR0862201.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7278..7673)
                     /gene="caiF"
                     /locus_tag="ACHUPG_002647"
     CDS             complement(7278..7673)
                     /gene="caiF"
                     /locus_tag="ACHUPG_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR0862202.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7935..11156)
                     /gene="carB"
                     /locus_tag="ACHUPG_002648"
     CDS             complement(7935..11156)
                     /gene="carB"
                     /locus_tag="ACHUPG_002648"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR0862203.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11174..12322)
                     /gene="carA"
                     /locus_tag="ACHUPG_002649"
     CDS             complement(11174..12322)
                     /gene="carA"
                     /locus_tag="ACHUPG_002649"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR0862204.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
BASE COUNT         2912 a         3256 c         3306 g         3002 t
ORIGIN      
        1 gaacgcaaaa taaatattcg ttttcacagt ggaattaact catgaagaat gaaaagagaa
       61 aaacgggaat agaaccgaag gttttctttc cgccattaat aatcgtcggc atactttgtt
      121 ggcttacagt cagagatctg gatgcggcga atgtcgttat taatgctgta ttcagttacg
      181 tcaccaatgt atggggatgg gcatttgaat ggtatatggt ggtgatgctt ttcggttggt
      241 tctggctggt gtttggcccg tatgccaaaa agcgtttagg taacgaaccg ccagaattta
      301 gcaccgccag ttggatcttt atgatgttcg cctcctgtac gtctgctgcc gtactgttct
      361 ggggatcgat tgagatctac tactacatct ccaccccgcc gtttggctta gaaccgaact
      421 cgacaggagc gaaagagttg gggctggctt acagcttgtt ccactgggga cctctgccgt
      481 gggccactta cagcttcctt tcagtcgcct tcgcttactt cttctttgtc cgcaaaatgg
      541 aagtgattcg ccccagctcg acactggtgc cgctggcagg tgaaaaacac gccaaagggt
      601 tgttcggtac catcgtcgac aacttctatc tcgtcgcctt gatcttcgcg atgggtacca
      661 gtctgggcct tgccacgccg ctggtgaccg agtgtatgca atggttgttt ggcattccgc
      721 ataccctgca actggacgct atcatcatta cctgctggat tatcctcaac gccatttgcg
      781 tcgcttgcgg tctgcaaaaa ggggtacgta tcgccagtga cgtgcgtagt tacctgagct
      841 tcctgatgct gggttgggtg ttcatcgtca gcggtgccag cttcatcatg aactacttca
      901 ccgattcggt ggggatgttg ctgatgtatc tgccgcgcat gttgttctat accgatccca
      961 tcgctaaagg cggcttcccg cagggctgga ccgtgttcta ctgggcatgg tgggtgattt
     1021 acgccatcca gatgagtatc ttcctcgccc gcatctcccg tggtcgtact gtgcgtgaac
     1081 tgtgcttcgg catggtgatg gggctgacag catccacctg gatcctgtgg accgtgctcg
     1141 gtagtaacac tctgctgttg atggataaaa acatcatcaa cattccaaat ctgatcgaac
     1201 agtacggtgt ggcgcgcgcc atcatcgaaa cctgggccgc tctgccactc agcaccgcca
     1261 ccatgtgggg cttcttcatc ctctgcttta ttgccaccgt tacgctggtt aacgcctgct
     1321 cttataccct ggcgatgtct acttgccgcg aagtacgcga tggtgaagaa ccaccgctgc
     1381 tggtgcgtat cggttggtca attctggttg gcattatcgg tattgttctg ctggcgctcg
     1441 gcggcctgaa accgattcaa accgccatta tcgccggagg atgcccgctg ttcttcgtca
     1501 acattatggt gacgctctcc tttattaaag acgcgaaaca gaactggaaa gattaattaa
     1561 cccccaaaat atcaagaggt tgaaagatgg attttaattt aaatgatgag caggaactgt
     1621 ttgtcgccgg tatccgcgaa ctgatggcca gcgaaaactg ggaggcctat tttgccgagt
     1681 gcgaccgtga cagcgtctac ccggaacgtt ttgtcaaagc actggcggat atgggtatcg
     1741 acagtctgct gatccctgaa gagcacggtg gtctggacgc ggggtttgtt actctcgccg
     1801 ccgtgtggat ggagctggga cgtctggggg caccaaccta tgtactgtac cagctgccgg
     1861 gcgggttcaa taccttcctg cgcgaaggca cacaagagca gatcgacaag attatggctt
     1921 tccgcggcac cggtaagcag atgtggaact cagcgattac cgaaccgggc gcgggctccg
     1981 acgtgggtag cctgaaaacg acttataccc gtagaaatgg taagatttat cttaatggta
     2041 gtaagtgttt tattaccagt agcgcctaca ccccgtacat cgtggtgatg gcgcgcgacg
     2101 gggcttctcc ggacaaacct gtctacaccg aatggtttgt tgatatgagc aaaccgggca
     2161 tcaaagtgac caaacttgag aagctcggtc tgcgtatgga tagctgctgt gaaatcacct
     2221 ttgacgatgt ggaactggac gagaaagaca tgttcggtcg ggaaggtaac ggctttaacc
     2281 gcgtcaaaga agagttcgac catgaacgtt tcctggtagc cctgaccaac tacggtacgg
     2341 cgatgtgcgc ctttgaagat gctgcgcgct acgccaacca gcgcgtgcag tttggcgagg
     2401 ctattggtcg tttccagttg attcaggaaa aattcgccca catggcgatc aaattaaact
     2461 ccatgaaaaa catgctgtat gaagcagcgt ggaaagcaga caacggcacc atcacctctg
     2521 gcgatgcagc gatgtgcaaa tacttctgcg ccaatgcggc atttgaagtg gtggatagcg
     2581 caatgcaggt gctgggcggt gtcgggattg cgggcaacca ccgcatcagc cgcttctggc
     2641 gtgacctgcg tgtagaccgc gtttccggag gatctgacga aatgcagatc ctgacgctgg
     2701 gtcgtgcggt gctgaagcaa taccgctaag tccgtgcgct gcctgatgcg acgctgacgc
     2761 gtcttatcag gcctacggga gcacgaatgt aagccggata aggcgtttac gccgcatccg
     2821 gcagtcatgc gctacataaa tttttcagga gctaataatg gatcatctac ccatgccgaa
     2881 attcggaccg ctggccggat tgcgcgttgt cttctccggt atcgaaatcg ccggaccgtt
     2941 tgccggacaa atgttcgcag aatggggcgc ggaagttatt tggatcgaga acgtcgcctg
     3001 ggccgacacc attcgcgttc aaccgaacta cccgcagctc tcccgccgca atttgcacgc
     3061 gctgtcgtta aatattttca aagatgaagg ccgcgaagcg tttctgaaat taatggaaac
     3121 caccgatatc ttcatcgaag ccagtaaagg tccggccttt gcccgtcgtg gcattaccga
     3181 tgaagtactg tggcagcaca acccgaaact ggttatcgct cacctgtccg gttttggtca
     3241 gtacggcacc gaggagtaca ccaatcttcc ggcctataac accatcgccc aggccttcag
     3301 tggttacctg attcagaacg gtgatgttga ccagccaatg cctgccttcc cgtataccgc
     3361 cgattacttt tctggcctga ccgccaccac ggcggcgctg gcagcactgc ataaagtgcg
     3421 tgaaaccggt aaaggcgaaa gtatcgacat cgccatgtat gaagtgatgc tgcgtatggg
     3481 ccagtacttc atgatggatt acttcaacgg cggcgaaatg tgcccgcgca tgagcaaagg
     3541 taaagatccc tactacgccg gttgcggtct gtataaatgt gccgacggct acatcgtgat
     3601 ggaactggtg ggcattaccc aaattgaaga gtgctttaaa gatattggcc tcgcacatct
     3661 actcggtacg ccggaaatcc cggaaggcac tcagcttatc caccgtatcg aatgccctta
     3721 cggcccactg gtagaagaaa aactcgatgc ctggctggcg gcacatacca tcgcagaagt
     3781 taaagaacgc tttgccgaac tgaatatcgc ctgtgccaaa gtactgaccg taccggaact
     3841 ggaaagcaat ccacagtatg ttgcccgtga atcaatcact cagtggcaaa cgatggatgg
     3901 tcgcacctgc aaagggccga acatcatgcc gaaattcaaa aataaccccg gacaaatctg
     3961 gcgcggaatg ccctcacatg gcatggacac ggctgccatt ttgaaaaata tcggctacag
     4021 cgaaaacgac attcaggagt tggtcagcaa aggtctggcc aaagttgagg actaaacgcg
     4081 tggcgtcagt cggcttgcct gactagcgcc cgcccaataa caaacgaaca aaaatggata
     4141 gaggtgcaat ggatatcatt ggcggacaac atctacgtca aatgtgggac gatctggcgg
     4201 acgtttacgg tcataaaacg gcgctgattt gtgaatccag cggcggagtc gttaaccggt
     4261 atagttatct tgagttaaat caggagatta accgcacggc aaacctgttt tatacgctgg
     4321 ggattcgcaa aggcaacaag gttgcactac atctcgacaa ctgcccggaa tttatctttt
     4381 gctggttcgg gctggcaaaa attggcgcga ttatggtgcc gattaacgcc cgcctgttac
     4441 gcgaggaaag cgagtggatc ttgcaaaata gccaggcgtg cctgctggtg accagtgcgc
     4501 aattttatcc tatgtatcaa cagattcagc aggaagatgc cactcaattg cggcacattt
     4561 gcctgacaga tgtggcactt cccgctgatg atggagtgag ttcatttact caactgaaaa
     4621 atcaacaacc tgccaccttg tgctatgcac cgccgctatc gactgacgat acggcggaaa
     4681 ttctcttcac ctccggcacc acctcccgac cgaaaggtgt ggtgattacc cattacaacc
     4741 tgcgcttcgc tggatattac tccgcctggc agtgtgcact gcgtgacgat gacgtctaca
     4801 tgacggtaat gcctgcgttt catatcgatt gccagtgtac tgcggcgatg gcggcgtttt
     4861 ctgccggggc cacctttgtg ctggtcgaga aatacagcgc ccgcgccttc tggggacagg
     4921 cgcagaagta ccgcgccacc attaccgaat gtattccgat gatgatccgt acgttgatgg
     4981 tgcagccgcc ttcagcgaac gatcggcaac accgcctgcg ggaagtgatg ttttatctca
     5041 acttgtcgga gcaggaaaaa gacgcatttt gtgaacgctt cggcgttcgc ttgctgacgt
     5101 cttatgggat gacggaaacc attgtgggca ttatcggcga tcgccctagc gataaacgac
     5161 gctggccgtc gattggtcgg gcggggtttt gctacgaagc ggagatccgc gacgatcaca
     5221 atcgcccgct cccggcaggt gagatcggtg aaatctgtat taaaggcgta ccagggaaaa
     5281 ccatcttcaa agagtatttt ctcaacccga aagccactgc gaaagtgctg gaagccgacg
     5341 gctggctaca taccggcgat accggatacc gcgacgaaga aggctttttt tatttcgtcg
     5401 atcgccgctg caatatgatc aaacgcggtg gcgagaatgt ctcctgcgtg gagctggaaa
     5461 atatcatcgc cacccatccg aaaattcagg acatcgtggt tgtgggtatt aaagattcga
     5521 ttcgcgatga agccatcaaa gcatttgtgg tgctgaatga aggtgaaaca ttgagcgaag
     5581 aggaattttt tcgcttctgc gaacaaaata tggcgaaatt taaagtgccc tcttatctgg
     5641 agatcagaaa agatctgcca cgtaattgct cggggaaaat aattagaaag aatctgaaat
     5701 aaatgaaacg tcagggaact accctgccag ctaataatca cgccctaaaa caatatgcat
     5761 tttttgcagg gatgttgtcg tccctaaaaa agcaaaaatg gagaaaagga atgagtgaat
     5821 cattacatct gacccgcaat ggatcaattc tggaaattac ccttgatcgc ccaaaagcga
     5881 atgctattga tgcaaaaacc agctttgaaa tgggcgaagt atttctaaat ttccgtgacg
     5941 atccgcaatt acgtgtcgcc attattaccg gtgccggaga gaaattcttt tccgcgggct
     6001 gggatttaaa agcggcagca gaaggcgaag caccggatgc tgactttggc ccgggtggtt
     6061 ttgcgggatt aaccgaaatt ttcaatctcg acaaaccagt tatcgcagct gtgaacggct
     6121 acgcctttgg cggcggtttc gaactggcgc tggcggcaga ttttattgtt tgtgccgata
     6181 acgccagctt cgccctgccg gaagccaaac tgggcatcgt tcctgacagc ggcggtgtgc
     6241 tgcgtctgcc gaagatcctg ccgcctacca tcgtcaatga aatggtgatg accggcagac
     6301 gaatgggcgc agaagaggcg ctgcgttggg ggatagtcaa ccgcgtggtt agccaggcgg
     6361 aactgatgga taacgcccgc gaactggctc agcagctggt taacagcgcc ccgctggcga
     6421 ttgcggcgct gaaagagatc taccgcacca ccagcgaaat gccggtagaa gaagcgtatc
     6481 gctatattcg cagcggcgtg ttgaaacact atccatcggt tctgcattcg gaagatgcca
     6541 ttgaagggcc gctggcgttt gccgagaagc gcgatccggt gtggaaagga cgttaacaac
     6601 cgtgagctat tacgcctttg aggggttaat tccggtggtt cacccgacgg cgtttgtcca
     6661 tcccagtgcc gtcttgattg gcgatgtgat tgtgggagcc ggtgtctaca tcggcccact
     6721 cgcctcactg cgtggtgact acgggcggtt gatcgtgcaa gcgggagcca atattcagga
     6781 tggctgcatt atgcatggct actgcgacac tgacaccatc gttggggaaa acggccatat
     6841 cgggcatgga gcgatcctgc atggttgtgt gattggtcgc gatgcattgg tcgggatgaa
     6901 cagcgtaatt atggatggcg cggtcattgg cgaagagagc attgttgccg caatgagctt
     6961 tgtcaaagca ggctttagtg gcgagaaacg ccagttgttg atgggtacgc cagcccgcgc
     7021 tgtacgcagt gttagtgacg acgagttaca ctggaaacgg ttgaatacca aagagtatca
     7081 ggatcttgtc gggcgctgtc atgcatcgtt acatgaaacg cagccactga ggcaaatgga
     7141 ggaaaatcgc ccccgtttgc aggggacgac ggatgtgacg cctaaacggt gatcttgccg
     7201 gatgcgacct ggttgctgga taagatgcta caacatcgta tctggcaagc aggacgcccg
     7261 gtatcaacga gataaaatta acgacgcata ctctttgaca acatcatctg ccacttctgc
     7321 tcgcggttaa gttccgtgag cggaacggca ggaatacgcg gtgtctttct tacgccgact
     7381 aatttttccc gactgttatt gcgcaggcgc gcgtaaattt gctcatcgat atcgaccact
     7441 ttaaccagtc gctgacactg gcatccccgc ccttccagct tattagggat catcttaact
     7501 tcgcagctta tttctgtgac ttccgacaga atataagtca gggtattaac cgccttgctg
     7561 tgttcaatat cgaaatgaat agagatctct cttgctatca cccaccgatt ttcagccatc
     7621 atccattcgg cgattaacaa gtagagtggt ttttcaacat atccttcaca catacgaact
     7681 ccagtttcac aaattaaaag tctagctatg actcgttttg aagcaattaa acaaggccat
     7741 attaaaattg tggatatatc gatagtatgc aacttcaccg tcgataagtg tgagctaaat
     7801 cctgcttatg tcatcaaaaa tatcgattcc ccaaaagatt tacttaatgg gcaaaaaaaa
     7861 acggtcctca tcagagaacc gtattagtta ttcgatcaaa ttagcggatg aaaaatatcc
     7921 gccacgacac gctattattt gatctgtgcg tgcatttcct gcaccgaaat tactttttca
     7981 gtcgcatcgg cattcagcgc catcgcggta gcgaaaccac cgttcagggt ggtgtcataa
     8041 tgcactttat attgcagcgc actgcgacgg atcacgcggg agtcttcaat cgcacgacgg
     8101 cctgaggtgg tgttgatgat gtaggtatat tcgccattct tgatacggtc ctgaatgtgc
     8161 ggacggcctt catgcacctt gtttaccaga cgcggattga tacccgcttc gcccagcaca
     8221 atcgccgtgc cgtgggtcgc atccagctcg aagccctgtt tcagcagttt tgccgccagg
     8281 tccaccacgc gttctttatc gccttcgcgc acggaaagca gcgcacgacc gtgtttcttc
     8341 atggtggagt tgctgcccag ctgcgctttg gcgaacgctt cagcgaaggt gcggcccacg
     8401 cccatgactt ccccggtaga gcgcatttct ggccctaaca gcgggtcaac gcctgggaat
     8461 ttattgaacg gcagcaccac ttctttcacc gagtagtacg gcggaataac ttctttggtt
     8521 acgccctgct cagccagcga tttgccagcc atcacacgcg ccgccacttt tgccagcggt
     8581 acgccggtgg ctttggagac gaacggaaca gtacgcgccg cacgcgggtt tacttcaatc
     8641 aggtaaactt cgttgttttt caccgcaaac tgcacgttca tcagaccgcg cacctgcaat
     8701 tcgaaggcca gtttctgcac ctgctggcgc atcacatcct gaatttcctg acttaaggtg
     8761 tacgctggca gcgaacatgc ggagtcaccg gagtgcacac ccgcctgctc gatgtgctcc
     8821 atgatgccgc caatcagcac catttcgccg tcgcagatgg catccacgtc aacttctact
     8881 gcgtcatcaa ggaaatgatc cagcaacact ggcgcatcgt tagacacgct aaccgccgtc
     8941 tggaagtagc gacgcaggtc agcttcgtca tagacgattt ccatcgcccg accgccgaga
     9001 acgtaagacg gacgcaccac cagcgggtag ccaatctctt ttgccttctc aactgccatt
     9061 tcaatagcag taacggtggc gttcgccggt tgtttcagtt tcagacggtc aaccgcatgc
     9121 tggaagcgtt cacggtcttc cgcacggtcg atagcatccg ggctggtgcc gataaccggt
     9181 acgccagcgg cttccagtgc acgcgccagt ttcagcgggg tctgaccgcc gtactgaacg
     9241 ataacgcctt tcggcttctc aatacgcacg atttccagca catcttccag agttaccggc
     9301 tcgaagtaga ggcgatcgga agtgtcgtag tcggtagaga cggtttccgg gttacagtta
     9361 accataatgg tttcgtaacc gtcttcacgc agcgccagcg aggcgtgtac gcagcagtag
     9421 tcgaattcga taccctgacc gatacggttt ggaccgccgc caagcaccat gattttttca
     9481 cggtcggtag acggattcgc ttcgcactct tcttcataag tggagtacat gtaagcggtg
     9541 tcggtggcaa actctgccgc acaggtatcc acgcgcttgt agaccgggtg caggtcatat
     9601 tggtcacgca gcttacggat ttccgcttcg cgtacgcccg ccagttttgc caagcgcgca
     9661 tcggcaaagc ctttgcgttt cagctggcgc aggaattcag cgttcaggcc agtgatgccc
     9721 acttccgcca ctttctcttc cagacgcacc agttcttcaa tctgtaccag gaaccagcgg
     9781 tcaatgttag tcaggttgaa gacgccgtcc acagacaggc ccgcgcggaa agcatcggcg
     9841 atgtaccaga tacgctctgc gcctgcgtct ttcagttcgc gacggatttt ggttaacgct
     9901 tccgggtcat ccaggctcac tttcgggtcg aagccagttg cgcccacttc caggccgcgc
     9961 agcgcttttt gcagggattc ctgctgcgtg cgaccaatcg ccatcacttc gccaaccgat
    10021 ttcatctgag tggtcagacg gtcgttagca ccggcgaatt tttcgaagtt gaagcgagga
    10081 attttagtaa ccacatagtc gatggacggc tcgaaggagg ccggagtacg tccgccagtg
    10141 atgtcgttca tcagttcgtc tagggtgtaa cccaccgcca gtttcgccgc cactttagca
    10201 atcgggaaac cggtcgcttt cgacgccagc gccgaagaac gggacacgcg tgggttcatt
    10261 tcgataacaa tcaggcgacc gtttttcggg ttcaccgcaa actggacatt ggaaccaccg
    10321 gtttcaacgc cgatttcacg cagcaccgcc atcgaggcgt tacgcatgat ttgatattct
    10381 ttgtcggtca gcgtttgggc tggcgcgaca gtgatggagt caccggtgtg gatgcccatc
    10441 gcatcaaagt tttcgataga gcagacgatg atgcagttgt cgtttttatc acgcaccact
    10501 tccatctcgt actctttcca gccgatcagc gactcatcaa tcagcaactc tttggtcgga
    10561 gagagatcca gaccgcgggc gcaaatttct tcaaactctt cacggttata agcgataccg
    10621 ccgccgctac cgcccatggt aaaggatggg cgaataatgc acgggaagcc cacgtcagcg
    10681 gcaacagcca gcgcttcttc catcgtgtgt gcgataccgg aacgcgcggt ttccagacca
    10741 attttcttca tcgctacgtc gaaacgacgg cggtcttctg ctttatcaat cgcatcggca
    10801 gtggcaccaa tcatggtgac accgaactct tccaacacgc cctgacgttc cagctccagc
    10861 gcgcagttca gcgccgtctg accgcccatg gttggcagca ccgcgtccgg gcgctctttt
    10921 tcaataatct tgcgtaccac ttcccagtga atcggctcga tgtaggtcgc atcggccatt
    10981 tccgggtcgg tcatgatggt cgccgggttg gagttcacca gaatgacgcg gtaaccctct
    11041 tcgcgcaggg ctttacatgc ttgcgcgcca gagtagtcaa actcacacgc ctgaccgata
    11101 acaatcgggc ccgcacccag aatcaggata ctttttatat ctgtacgttt tggcatggct
    11161 cttttactcc tgattactta gcggttttac ggtactgctc aattaacttg ataaagtggt
    11221 cgaacaacgg cgcggcgtcg tgtggaccag ggctggcttc agggtgaccc tggaagctga
    11281 acgccggctt atcgatgcga tgaatgccct gtaacgtacc gtcgaacagg gatttatgcg
    11341 tgacacgcag gtttgcaggt aatgttgctt catccaccgc aaaaccgtgg ttctgggcgg
    11401 tgatcatcac cacgtttttc tccacatctt taaccggatg gttgccgccg tggtgaccaa
    11461 atttcatttt gacagtcttc gcaccgctcg ccagcgccag cagctgatga ccgagacaga
    11521 tgccgaatac cggaatatcg gtttcgagga acttctggat ggcggtaatg gcgtaatcgc
    11581 acggggccgg gtcgccagga ccgttggaga ggaagatgcc gtctggattc attttcagca
    11641 catcttccgc agaagtttgc gccggaacga tggtcaggcg acagcctcta tccaccagca
    11701 tccgcaggat gttgcgcttg gcaccaaaat cataagccac gacgtggaac ggcagctcgt
    11761 cttctttttt cgcttctggc aggccgccgg tcaacgtcca gctcccttgt gtccagctat
    11821 aggcttctgc ggtggtcact tcttttgcca gatccatgcc gttcagacct gggaacgcgc
    11881 gggctttttc taacgccagc gccgcatccg ggttatcgcc tgcgataatg cagccattct
    11941 gtgcgccttt ctcgcgcagt aaacgcgtca gcttacgggt atcgatatcg gcaatcgcca
    12001 cgatgttatg gcgcttcagg taagaagaga ggtcttcggt attacggaag ttgctggcaa
    12061 tcagcggcag gtcgcgaatc accagacctt gtgcatgtac ctgagaagat tcttcatcgg
    12121 cgtcattagt gccgacattg ccaatatggg gataagtaag agtaacgatt tgacgagaat
    12181 aggaaggatc agtgaggatt tcttgataac cggtcattga agtattgaaa acgacttccc
    12241 caaccgccga acctgttgcc cctatggccc gaccgtgaaa ctgggttccg tcttccagaa
    12301 ccaatagcgc tgacttaatc aaaacaccct ccagagaata ctcactcact ttatttgcat
    12361 attaattcac agtgatgacg taaatcaatg caaatctgct taccgacgaa tttctggcaa
    12421 acggcggcat tctggagata tgggcgctaa aagtcaactt aatggcagga tttttt
//
LOCUS       DAZPAB010000106        12393 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZPAB010000106 DAZPAB010000000
VERSION     DAZPAB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.106"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="ACHUPG_002650"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="ACHUPG_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR0862205.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     misc_feature    547..670
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            717..1616
                     /gene="hisG"
                     /locus_tag="ACHUPG_002651"
     CDS             717..1616
                     /gene="hisG"
                     /locus_tag="ACHUPG_002651"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR0862206.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1622..2926
                     /gene="hisD"
                     /locus_tag="ACHUPG_002652"
     CDS             1622..2926
                     /gene="hisD"
                     /locus_tag="ACHUPG_002652"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR0862207.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2923..3993
                     /gene="hisC"
                     /locus_tag="ACHUPG_002653"
     CDS             2923..3993
                     /gene="hisC"
                     /locus_tag="ACHUPG_002653"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR0862208.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3993..5060
                     /gene="hisB"
                     /locus_tag="ACHUPG_002654"
     CDS             3993..5060
                     /gene="hisB"
                     /locus_tag="ACHUPG_002654"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR0862209.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5060..5650
                     /gene="hisH"
                     /locus_tag="ACHUPG_002655"
     CDS             5060..5650
                     /gene="hisH"
                     /locus_tag="ACHUPG_002655"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR0862210.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5650..6387
                     /gene="hisA"
                     /locus_tag="ACHUPG_002656"
     CDS             5650..6387
                     /gene="hisA"
                     /locus_tag="ACHUPG_002656"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR0862211.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6369..7145
                     /gene="hisF"
                     /locus_tag="ACHUPG_002657"
     CDS             6369..7145
                     /gene="hisF"
                     /locus_tag="ACHUPG_002657"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR0862212.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7139..7750
                     /gene="hisIE"
                     /locus_tag="ACHUPG_002658"
     CDS             7139..7750
                     /gene="hisIE"
                     /locus_tag="ACHUPG_002658"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR0862213.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="ACHUPG_002659"
     CDS             complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="ACHUPG_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR0862214.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="ACHUPG_002660"
                     /pseudo
     CDS             complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="ACHUPG_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="ACHUPG_002661"
     CDS             complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="ACHUPG_002661"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR0862215.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11944..>12393)
                     /locus_tag="ACHUPG_002662"
     CDS             complement(11944..>12393)
                     /locus_tag="ACHUPG_002662"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862216.1"
                     /translation="QVGTIFYPCVYYFFSGRSLYFEFKYFYSEYLNFNSRLESRYSYE
                     LMKKASEYSELYGDNLIQLGLEDGIYFYKGMAIGDVFGLARYSDWTISNPECEVIPQD
                     DLIEKMKSFNSSFIVISKRSYANFNPEKYPKFKVLMDTPNGILIAIK"
BASE COUNT         3145 a         3140 c         3087 g         3021 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgaa ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
      601 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
      661 gcttttttat tgcgcggttg ttaacggttc agacaggttt aaagaggaat aagaaaatga
      721 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
      781 gcgaattact ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
      841 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgatgacatt ccctgtctgg
      901 taatggacgg cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa gaagagctgc
      961 ttaaccgccg cgcccagggt gaagatccgc gctactttac cctgcgtcgt ctggatttcg
     1021 gcggctgccg cctttcgctg gcaacgccgg ttgatgaagc ctgggacggt ccgctctcct
     1081 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     1141 aaggtatctc ttttaaatcc tgcttactga acggttctgt cgaagttgcg ccgcgtgccg
     1201 ggctggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     1261 tgcgcgaagt cgaagttatc taccgctcga aagcctgcct gatccagcgc gatggcgaaa
     1321 tggaagaatc taaacagcaa ctgatcgaca agctactgac ccgtattcag ggtgtgatcc
     1381 aggcgcgcga atcaaaatac atcatgatgc acgcaccgac cgaacgcctg gatgaagtca
     1441 tcgccctgct gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt gatcaacagc
     1501 gcgtagcgat gcacatggtg agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     1561 aagcactggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     1621 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     1681 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcaccgtta acgatattct
     1741 cgataacgtg aaaacgcgtg gcgatgaggc cctgcgtgaa tacagcgcga agtttgataa
     1801 aaccacggtt accgcgctga aggtgtctgc tgatgagatc gccgccgcca gcgaacgcct
     1861 gagcgacgag ctaaaacagg cgatggcggt ggcagtaaag agtattgaaa ccttccacac
     1921 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcta ggcgtgcgtt gccagcaggt
     1981 cacgcgcccg gtggcttcag tgggtttgta tattccgggc ggctccgccc cgctcttctc
     2041 aacggtatta atgctggcaa ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     2101 ctcaccgccg ccgattgccg atgaaattct ttatgcggcg cagctgtgcg atgtgcagga
     2161 cgtgtttaac gtcggcggcg cacaggccat tgccgcgctg gcgtttggta cggaatctgt
     2221 gccgaaagtg gacaaaatct tcgggccggg taacgccttt gtcaccgaag caaaacgcca
     2281 ggtaagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgct
     2341 ggtgattgct gacagcggcg ctacgccgga ttttgtggct tctgatttgc tttctcaggc
     2401 tgaacacggc cctgactcac aggtaatttt actgaccccc gatgctgata tggcgcgtcg
     2461 cgttgccgag gccgtcgaac gccaactggc agaactgccg cgtgccgaaa ccacccgcca
     2521 ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tggagatctc
     2581 caaccagtac ggcccggagc acctgatcat tcagacccgc aacgcccgcg aactggtcga
     2641 tggcatcacc agcgccggtt cggtatttct tggtgactgg tcaccggaat cggcaggcga
     2701 ctacgcctcc ggcaccaacc acgtgctgcc gacttacggt tacaccgcca cctgttccag
     2761 cctcgggctg gcagatttcc agaaacgcat gaccgtacag gaactgtcga aagaaggttt
     2821 ctccgcgctg gcttcgacca ttgaaacact ggccgccgcc gagcgcctga ccgcccacaa
     2881 aaatgccgtt actttgcgtg ttaacgtcct taaggagcaa gcatgagcac cgtgactatt
     2941 actgatttag cgcgtgaaaa cgtccgcaac ctgacaccgt atcagtcggc gcgtcgtctg
     3001 ggcggtaacg gcgacgtctg gctgaacgcc aacgaatatc ccacagccgt ggagtttaag
     3061 cttactcagc aaacgctcaa ccgctacccg gaatgccagc caaaagcggt gattgaaaat
     3121 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     3181 ggtattgaac tgctgatccg cgctttttgc gaaccgggta aagacgccat cctctactgc
     3241 ccgccaacgt acggcatgta cagcgtcagc gctgaaacca ttggtgtcga atgccgcaca
     3301 gtgccgacgc tggaaaactg gcaactggac ttacagggca tttccgacaa gctggacggc
     3361 gtaaaagtgg tctatgtttg cagccccaac aaccccaccg ggcaactgat caatccgcag
     3421 gattttcgca ccctgctgga gctaacccgc ggtaaggcga ttgtggttgc cgatgaagct
     3481 tatatcgagt tttgcccgca ggcatcgtta gctggctggc tggcggaata tccgcacctg
     3541 gcgattttgc gtaccctttc gaaagccttc gcgttggcgg ggcttcgttg cggatttacg
     3601 ctggcaaacg aagaagtcat caacctgctg atgaaagtga tcccccccta cccgctctcg
     3661 acgccggttg ccgacattgc cgcacaggcg ttaagcccgc agggaatcgt cgccatgagc
     3721 gaacgggtgg cgcaaattat tacagaacgc gaatacctga ttgccgcact gaaagagatc
     3781 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     3841 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     3901 caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     3961 gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt tttatcgatc
     4021 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgacaaac
     4081 tcgcctttga accgggcgtg atcccggaat tgctgaagct gcaaaaagcg ggctacaagc
     4141 tggtgatgat cactaatcag gatggtctgg gaacacaaag tttcccgcaa gcggattttg
     4201 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     4261 tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac
     4321 tggtggaggg ttatctggct gagcaagcga tggatcgtgc caacagttat gtaattggcg
     4381 atcgcgcgac cgacattcaa ctggcggaaa acatgggcat taatggttta cgctacgacc
     4441 gcgaaaccct gaactggcca atgattggcg agcaactcac taaacgagac cgttacgccc
     4501 atgtagtgcg caacaccaaa gagacgcaga ttgacgtcca ggtgtggctg gatcgcgaag
     4561 gtggcagcaa gattaacacc ggcgttggct tctttgatca catgctggat cagatcgcca
     4621 cccacggcgg tttccgcatg gaaatcaatg tcaaaggcga cctctatatc gacgatcacc
     4681 acaccgtcga agataccggc ctggcgctgg gcgaagcgtt aaaaattgcc ctcggcgaca
     4741 aacgcggtat ttgccgcttt ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg
     4801 cactggatat ctctggtcgc ccgcacctgg aatataaagc cgagtttacc taccagcgtg
     4861 tgggcgatct cagcactgaa atgatcgaac acttcttccg ttcgctctct tacaccatgg
     4921 gcgtgacact acacctgaaa accaaaggta aaaacgatca tcaccgtgta gaaagcctgt
     4981 tcaaagcctt tggtcgtacc ctgcgccagg ccatccgcgt cgaaggcgac accctgccct
     5041 cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat actggctgcg ccaacctgaa
     5101 ctctgtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     5161 cattgtgttg ctggccgata aactgttttt acccggcgtc ggcaccgcac aagcagcgat
     5221 ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtgct
     5281 gggcatctgc ttagggatgc aactcctggg acgacgcagc gaagagagca atggcgtcga
     5341 cttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc tgccactgcc
     5401 acatatgggc tggaaccgcg tttatccgca ggcaggcaac cgcctgtttc aggggattga
     5461 agacggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
     5521 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
     5581 cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
     5641 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
     5701 cagggcgatt acggcaaaca acgcgattac ggtaacgacc cgctgccgcg cttacaggat
     5761 tacgctgcgc aaggtgccga agtgttgcac ctggtggatc tgaccggggc aaaagatccg
     5821 gctaagcgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt cccggtgcag
     5881 gttggcggcg gtgttcgtag tgaagaagac gtggcggcgt tactggaagc tggcgttgcg
     5941 cgcgtggtgg tcggttccac cgcagtgaaa tcaccggacg tagtgaaagg ctggtttgaa
     6001 cgtttcggtg ccgatgcgct ggtgctggca ctggatgtcc gtattgacga gcaaggcaac
     6061 aagcaagtgg cagtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
     6121 gaaacctatc tgcccgtcgg cctgaaacat gtgttgtgta ccgatatctc gcgcgacggc
     6181 acgctggcgg gatctaacgt ctctttatat gaagaagtgt gtgccagata tccgcaagtg
     6241 gcgtttcagt cctccggcgg cattggcgac attaacgatg ttgctgccct acgtggcact
     6301 ggcgtgcgcg gcgtaatagt tggtcgtgca ttactggaag gtaaattcac cgtgaaggag
     6361 gccatcgcat gctggcaaaa cgcataatcc cgtgtctcga cgttcgtgat ggtcaggtgg
     6421 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgcca ctggcaaaac
     6481 gctacgccga agaaggtgca gatgaactgg tgttctacga tatcaccgct tccagcgatg
     6541 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgtttt
     6601 gtgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
     6661 ataaaatttc catcaactcc cctgcgctgg cagacccaac attaattact cgcctggccg
     6721 atcgctttgg cgtgcagtgt attgtggttg gtattgatac ctggtacgac gccgaaaccg
     6781 gtaaatatca tgtaaatcaa tataccggcg atgaaagccg cacccgcgtc actcaatggg
     6841 aaacgctcga ctgggtagag gaggtgcaaa aacgcggcgc cggagaaatc gtcctcaaca
     6901 tgatgaatca ggacggcgtg tgtaacggtt acgacctcaa gcagctgaaa aaagtgcgtg
     6961 aagtttgcca cgtcccgctg attgcctccg gtggcgcggg caccatggaa cacttcctgg
     7021 aagccttccg cgatgccgac gttgacggtg cgctggcagc ttccgtattc cacaaacaaa
     7081 taatcaatat tggcgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
     7141 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggtc tgatgccggt
     7201 gattgtgcaa cacgcggtat ctggcgaagt gttaatgctg ggctacatga acccggaagc
     7261 cttagacaaa accatcgaaa gcggtaaagt caccttcttc tcgcgcacta aacagcgact
     7321 gtggatcaaa ggcgaaacct cgggcaattt tctcaatgtg gtaagtattg ccccggactg
     7381 cgacaacgac acattactgg tgctggcgaa tcccatcggg ccaacctgcc acaaaggcac
     7441 cagtagctgc ttcggcaaca ccgctcacca gtggctgttc ctgtatcaac tggaacaact
     7501 gctcgccgaa cgtaaatatg ccgacccgga aacctcctac accgccaaac tgtatgccag
     7561 cggcactaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggcagc
     7621 aacggtacat gatcgctttg agctgaccaa cgaggcatct gacttgatgt atcacctgct
     7681 ggtgctgttg caggatcagg atctggattt aacgacagta attgagaacc tgcataaacg
     7741 gcatcagtga gttacgggct gagcaggtgt gatgttgttg tcacacccgg agccattgcc
     7801 cgatgcgcaa acttatcgtg cctacacatt atctcaacac acgtaggccg gataagatgc
     7861 gccagcatcg catccggcaa aaaaaacggg catggtgtca ccaccctgcc cgtttctctt
     7921 aaatgcacaa taatattact tcgcgttgta attacgcaga gcgttacgcc ccaacacaat
     7981 ccccgcgcca accatgccgc ccagaagcac tgccagaatc aaggtgattg cctttttcgg
     8041 actatcgcga cgaataggta acgtcggttt catcacatag cggtaagcat gaatatcaag
     8101 atcatcaaac tttaattttt caatatccaa caggttttga cgtgtctgat agtagtttgg
     8161 tgagaacacc aacggacgag tcgcttcatg cttaatcatc gactccagcg cttcgctccc
     8221 tagaaggaac aacgtatctt gcgtcacatc ttcagtctgc tgaacctgtg gctttgtcac
     8281 ctgcgcctga ttcgcatact gcaacgcttc ctgaatctga cggatacgca gatctttctg
     8341 ctcctgcgcg accacttcct gggttcttaa agagtcctgc aagtttttcc gtcccagagc
     8401 aatgttgtcc ttgagatcct tttctagctc ttgattcact ttatcatcaa cttgctgaat
     8461 gtattgggcc aacttcattt gtgcgccctc tgcagtttgc ccaacataag aaacagtcaa
     8521 tggtaattgc tggttcttaa cagaaggttc gatggtaagt ttttctggct cttcctgatt
     8581 atccagcgtt tctgctaatg cagagaaggc agaactgaag cgaccaatta acgtctcctg
     8641 caaatccgat actttcggtg cagcctgacc atagataaca ttcatggcat tgttatagcc
     8701 agcaatttgc cccacgtcgg gctgagtgat aattgctgtt gacgtccatt tctccttcgc
     8761 tactgccaaa taaccaatag ccagggcaat agccacaatg acggaaatga taattgtcat
     8821 cttgccacgc cacaactgca ctagtaaatc aatcaaatca atctgttccg ggtcatggtt
     8881 ttgcccagaa acattattat tttctactct catcattacc ctaactgacg gaaatttcca
     8941 agtccgcata gtttagcgat aatccccgtg aatgctatag gataagtgat aaaaataatg
     9001 aattattgcg gaggtaacaa aagacaatca gggcgtaaat agccctgata acaagatgtt
     9061 agtcgctgcc aaagagatcg cgggtgtaga ccttatctgc cacatcctta agctcttctg
     9121 ccatacggtt ggagataatg acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca
     9181 ggcgagagtt gaagaatgag tcttctttca tcaccggctc gtagatgatc acttcaacgc
     9241 ctttcgcctt gatacgcttc ataatcccct gaatagaaga cgcacggaag ttatctgacc
     9301 cgctcttcat aatcagacga taaataccca ccacttgcgg tttgcatcga caattgccga
     9361 gatcaggtta ttcggcacag actggtagtt cgccagtaac tgcttggtat ctttcggcag
     9421 acaataacca ccataaccaa acgacggatt gttgtaatgg ttgccaatac gcggatcgag
     9481 acaaacgcct tcgataatct ggcgagtatt cagacctaaa ctttctgcat aactatccag
     9541 ttcattaaag tatgctacgc gcatcgccag ataggtatta gcgaaaagtt taatcgcttc
     9601 tgcttcagtg gagtcggtaa acagggtcgg gatattttgc ttaatcgcgc cttcctgtaa
     9661 caacgcagca aaacgttctg cgcgttctga acgctcaccg atgacaatac gtgacggatg
     9721 gagattatcg taaagggctt taccttcgcg gaggaattcc ggagagaaaa taatattttc
     9781 agtacgatat ttcttatgca tcgctgcggt aaaaccaacg ggaaccgttg atttgatgac
     9841 cataaccgca taaggattta tctcaactac gtctttaatt actgattcta cactggatgt
     9901 attgaaataa ttagttttag gatcatagtc ggttggagtg gcgatgatga cataatcagc
     9961 atcccggtag gcttcatttt tatctaatgt ggcattaaag tgtattttat ctgattgcaa
    10021 aaactgctga atttccttat caacaatagg agatatccga tcattcagca tagcaacgcg
    10081 tgacggtaaa atatctaatg ccacaacctc atgattttgt gcgattagaa gcccgtttga
    10141 caagcctaca tagccagtac cggaaatggt gattttcatt tcatgctctc agaattaact
    10201 taactgtgaa tcatgatgtt tttagcatcg tgataagatg ctaaaagttt taacgctacg
    10261 ttgtcagatg gtcaacgcaa cgaaaaacaa ttcagatttt ctttataaga atagctgatg
    10321 tttattaagt taatattaag cagtgaaatt tagtctataa gatatttggc aaaaaaaagc
    10381 ccggtgcaat atacgccggg cctcaatttt attgttggtt aaatcagatt aatccagcca
    10441 ttcagtatgg aacacacctt ctttatcaat gcgcttataa gtatgcgccc cgaaatagtc
    10501 acgctgtgcc tggatcaggt tcgcaggcag aaccgcggca cggtagctat cgtaataggc
    10561 aaccgcagcg gcgaaggtcg gaaccgggat accgttctgt actgcataag cgacgacatc
    10621 gcgcagcgcc tgctggtagt catcggcaat ttgcttgaag tacggggcca gcagcaggtt
    10681 agcgatctgc ggattttcgg cataagcatc ggtgattttc tgcaggaact gcgcacggat
    10741 gatgcagcca gcacggaaaa tcttcgcgat ttcgccgtag ttcagatccc aattgtattc
    10801 ttcagacgca gcacgcagct gagagaagcc ctgagcgtaa gaaacgattt tgcccagata
    10861 cagcgcactg cgaacttttt cgatgaactc agccttgtcg cctgctgact gagcttgcgg
    10921 accagagaga actttagatg cggcaacgcg ctgatctttc agagaagaga tataacgtgc
    10981 aaacacagac tcggtaatca gcgacagcgg ttcgccgaga tccagcgcgc tctggctggt
    11041 ccatttaccg gtgcctttgt ttgctgcttc atccagaatc acatcaacca ggtagttacc
    11101 gtcttcatct tttttggtga agatatcttt agtgatgtcg atcaggtagc tgctaagttc
    11161 accgttattc cactcggtga aagtctgggc cagttcttca ttagatagat tcaggccacc
    11221 tttgagcaga gaatacgctt cagcaatgag ctgcatatct ccgtattcaa taccattgtg
    11281 aaccatcttc acataatggc ctgcgccgtc ggcaccaata taagtaacgc atggctcgcc
    11341 gtcttctgcc acggcagcga ttttagtcag gataggcgca accagctcat aagcctcttt
    11401 ctgcccgcca ggcataatag aaggaccttt cagcgcgccc tcttcaccgc cggaaacacc
    11461 ggtaccgatg aagttaaagc cctctgctga aagctcacga ttacgacgaa tggtgtcctg
    11521 gaagaaggta ttaccaccat caatgatgat gtcaccttta tcgagatatg gtttcaggga
    11581 atcaatcgca gcatccgtgc ctgcacctgc tttcaccatt aacaggatgc gacgaggcgt
    11641 ttccagagat tcaacaaact ctttcaccgt atagtaagga gccagtttct tgcctggatt
    11701 ttcagcaatc acttcttccg tcttttcgcg ggagcggttg aaaatagaga cggtataacc
    11761 acgactttcg atgttgagcg caaggttgcg ccccatcact gccataccga ctacgccgat
    11821 ctgttgcttt gacattgttt actcctgtct tctatattat taaccgtcta ttaaccgtta
    11881 tattattaac cgtcgtgatt ttcatcttct aatcaaggaa tacatatggt tacagcatgc
    11941 ttcttacttt atagcgataa gtatgccatt gggtgtatcc ataagaactt tgaattttgg
    12001 atacttttct ggattaaaat tagcatagct tcgtttagat ataacaataa acgaactatt
    12061 aaaagatttc atcttctcta ttaaatcatc ctgtgggatt acctcacact cgggattact
    12121 aatagtccaa tcactatatc ttgctaatcc aaagacgtca ccaattgcca tccctttata
    12181 gaagtaaata ccatcctcca agcctaattg gataagatta tctccatata actcactata
    12241 ttcacttgct ttcttcatca gttcatagct atagcgactt tctaagcgtg aattaaaatt
    12301 caagtattca gaataaaaat atttgaattc aaaatacaac gatcgccccg aaaaaaaata
    12361 atacacacaa ggataaaata tcgtcccaac ttg
//
LOCUS       DAZPAB010000107        12295 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZPAB010000107 DAZPAB010000000
VERSION     DAZPAB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.107"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            758..841
                     /gene="dinQ"
                     /locus_tag="ACHUPG_002663"
     CDS             758..841
                     /gene="dinQ"
                     /locus_tag="ACHUPG_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR0862217.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(895..2247)
                     /gene="gorA"
                     /locus_tag="ACHUPG_002664"
     CDS             complement(895..2247)
                     /gene="gorA"
                     /locus_tag="ACHUPG_002664"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR0862218.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="ACHUPG_002665"
     CDS             complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="ACHUPG_002665"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR0862219.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3364..5406
                     /gene="prlC"
                     /locus_tag="ACHUPG_002666"
     CDS             3364..5406
                     /gene="prlC"
                     /locus_tag="ACHUPG_002666"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR0862220.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5414..6166
                     /gene="rsmJ"
                     /locus_tag="ACHUPG_002667"
     CDS             5414..6166
                     /gene="rsmJ"
                     /locus_tag="ACHUPG_002667"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR0862221.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6215..7683)
                     /gene="dtpB"
                     /locus_tag="ACHUPG_002668"
                     /pseudo
     CDS             complement(6215..7683)
                     /gene="dtpB"
                     /locus_tag="ACHUPG_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            7849..7902
                     /locus_tag="ACHUPG_002669"
     CDS             7849..7902
                     /locus_tag="ACHUPG_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862222.1"
                     /translation="MVLNFVLAGDFDCVRRS"
     gene            complement(8001..8435)
                     /gene="uspA"
                     /locus_tag="ACHUPG_002670"
     CDS             complement(8001..8435)
                     /gene="uspA"
                     /locus_tag="ACHUPG_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR0862223.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8829..9164
                     /gene="uspB"
                     /locus_tag="ACHUPG_002671"
     CDS             8829..9164
                     /gene="uspB"
                     /locus_tag="ACHUPG_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR0862224.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9235..10734)
                     /gene="pitA"
                     /locus_tag="ACHUPG_002672"
     CDS             complement(9235..10734)
                     /gene="pitA"
                     /locus_tag="ACHUPG_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR0862225.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10966..12165
                     /locus_tag="ACHUPG_002673"
     CDS             10966..12165
                     /locus_tag="ACHUPG_002673"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR0862226.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3003 a         3123 c         3302 g         2867 t
ORIGIN      
        1 taacagaaat gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga
       61 taagtcaaaa acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg
      121 aattttgagt tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact
      181 ttgcgtcggt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
      241 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
      301 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
      361 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
      421 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
      481 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
      541 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
      601 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
      661 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
      721 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
      781 tcttggggcg ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
      841 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
      901 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
      961 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     1021 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     1081 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     1141 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     1201 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     1261 cttgttatta aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     1321 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     1381 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     1441 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     1501 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     1561 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     1621 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     1681 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     1741 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     1801 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     1861 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     1921 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     1981 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     2041 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     2101 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     2161 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     2221 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     2281 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     2341 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     2401 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     2461 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     2521 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     2581 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     2641 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     2701 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     2761 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     2821 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     2881 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     2941 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     3001 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     3061 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     3121 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     3181 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     3241 aggccgccgc cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc
     3301 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     3361 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     3421 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     3481 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     3541 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     3601 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     3661 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     3721 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     3781 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     3841 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     3901 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     3961 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     4021 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     4081 agcacccgcg cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg
     4141 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     4201 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     4261 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     4321 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     4381 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     4441 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     4501 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     4561 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc
     4621 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     4681 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     4741 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     4801 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     4861 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     4921 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     4981 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     5041 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     5101 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     5161 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     5221 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     5281 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     5341 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     5401 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     5461 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     5521 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     5581 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     5641 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     5701 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     5761 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     5821 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     5881 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     5941 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     6001 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     6061 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     6121 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     6181 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     6241 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     6301 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     6361 ccggcaccgc ggtaaatgtt gccacatagc cgccagcaag aacgcggcag cctgcgtcag
     6421 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
     6481 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
     6541 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagccgt
     6601 caaaaagccc agtgagcaca taaacatgct gagagtaaat ttcatcggca tcgagaggtc
     6661 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
     6721 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
     6781 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
     6841 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
     6901 atccagcttg aatgcctgac gaaagaagat gatagtgacg acgatggaga gaacaatcag
     6961 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
     7021 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
     7081 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
     7141 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
     7201 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
     7261 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
     7321 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
     7381 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
     7441 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
     7501 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
     7561 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
     7621 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
     7681 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
     7741 gaaaaagttt acatctgtca gagattctta atattgatgc ggtgaataga ttaatacact
     7801 attacaacag aaaataacca gctgatcaca ctaatgatgg aaaatatgat ggtgttaaat
     7861 tttgtattgg caggagattt tgattgcgta cgcagatcgt aggccagata aggggtttac
     7921 gctgcatcag gtaacaaggc gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg
     7981 aacacgtcgt ggagggatga ttattcttct tcgtcgcgta gcggaacaat cagcatatca
     8041 acgtgaacgg tgttgatcag ctgacgtgcg gaagacatca gtttgctcca gaagtcctgg
     8101 tggtgaccac aaaccaccag atccatatcg tatttcttga ttgcatcgac cagaacctgg
     8161 cctaggtcgc cgctgccgct cagggtttca gtgattggat agcctgcatt agtggaaagc
     8221 tcggtcagcg catgatgtgt ctcttcagag atgcgtttct gcatatcacc cagattcaca
     8281 tcaataagcc cggtgtatag gtcagagtag tttacatcta cgtggatcag agaaactttc
     8341 gcattgtagg ggcgagccat agagactgct ttctctacca gaactttgct ttccggggag
     8401 agatcgactg cgatgagaat gtgtttataa gccatagtgt tactccttcc ataaagttgt
     8461 cgatgactgg ccagctagcg tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg
     8521 tgcgcggggc taacccagcc gtaaagattc agtgttaaga ccatccttac cttatagcga
     8581 cccggatgat ccgtcaatcc gccttgctta cccgttagat aaacaaattt tcgacatgaa
     8641 tattatattg atagtggtta accttctgga aaaaaaacaa cctgatctcc tacactatct
     8701 atagagccgc tcggatgttg gtcaccacaa aagcggtttc tccaggacct ggtgttaact
     8761 gattggcagc gtatcgggag cggtagcccc ggggtggaac tccgcgggca ggtcgccggg
     8821 gaggaggtat gataagcacc gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta
     8881 acatggcgcg ctatttctca tcactacgcg cgttgttagt ggtactgcgt aactgcgatc
     8941 cattgctcta tcaatatgtt gatggagggg gcttttttac ctcacatggc caacccaaca
     9001 aacaggtgcg tctcgtttgg tatatctatg cccaacgtta tcgcgatcat cacgatgatg
     9061 agtttattcg ccgctgtgag cgggtgcgtc ggcagtttat tctgactagc gcattgtgtg
     9121 gtctggtggt ggtcagcctg attgcattga tgatttggca ttaagcactt cagcaataat
     9181 aacgtataaa aaaagcgggc cagtaaactg acccgctcgt tttggtgcgt acgattacag
     9241 gaactgcaag gagaaccagt acagcccgcc ggaaagtagt accgcagccg gaagggtaaa
     9301 cacccaggcc atcagaatac tggttacggt tttacgctgt aagccgccgc catctaccac
     9361 catcgtcccc gcgacagaag aggagagtac gtgagtggtg gaaaccggca tcccggtata
     9421 actcgccagg ccgattgaca ctgccgccgt catctgggca gacatcccct gagcgtaggt
     9481 catgcctttc ttaccgattt tctcaccgat agtcgttgcc acacgacgcc agccaatcat
     9541 cgtaccgata cctaacgcca gcgcgaccgc catgatgatc cacaccggcg catactcgat
     9601 ggtgctaagc atgtcggact tcagtttctt caacaggcgc tgatcgtcag cactcacacc
     9661 aggcatcttc accactttgt cgatagtgtc agaaacgcac agcataatgc ggcgcatctg
     9721 gctacgttga tcaagcgaca gcttgtcgta gctttccaca tcggtggtca acataccttt
     9781 caggcggttg agcgcgttaa tggtattcga cggatggcag tggaactccg caggttgcgt
     9841 tgcgccagct tccggagccg gtactaactg atcagcaccg gtagcctgtt tgagcagcgc
     9901 aggatgctgc tcaaagtaag cttcgacgtt gttgatggca tcacgggtac gggtgatttc
     9961 gtagccagtg gcattcatgt tcaccacgaa gcctgctggc gcgacgccaa tcaataccaa
    10021 cataaccaga ccaatgcctt tctgaccatc gttcgcgccg tgcgaaaacg ccacgccgat
    10081 agcggaaagg atcagcgcga tacgcgtcca gaacggcggc tttttcttgc cgtctttctt
    10141 ttcacgctcc gctggggtca ggtggatacg ggcgcgtttc ttggtgccgc tccagtagcg
    10201 acgcagcaag aaaatcagac cgccagcaaa caccaggccg acaatagggg aaacgatcag
    10261 agaaccgaaa atacttaata ctttcgggat attgagtgca tccaccactg acgtcccggt
    10321 catcaacgca ttggttaaac caatcccgat gatcgcgcca atcagcgtat gagagctgga
    10381 tgcaggtaaa ccaaagtacc aggtacccag gttccagata atcgccgcca gcaacataga
    10441 gaacaccatg gcaaggccat gagacgatcc catattaagc agcagatccg tcggcagcat
    10501 atgcacaatg gcataggcaa cactcagacc acccagcaaa acacccaaaa agttgaacac
    10561 cgccgccata accacggcga gctgagaacg catcgcgcgg gtatagataa cggttgccac
    10621 ggcgttggct gtgtcatgga aaccattgat ggcttcgtag aacagcacaa aagccagtgc
    10681 aagcaataat aacagcccgg tatgcaaatc caggccagca aacaaatgta gcataggacg
    10741 ttacgccatt ttgaggacat gaacgcggcg cattatcagt gactttcgcg gcgcgggcaa
    10801 agtgaaatat agactttttt tgatttgcct cctgtatgga tttcactcaa aaaataatta
    10861 tcttatataa ttcaggcaaa tacttccttt tagtaatatt gatgctggtg cgaccactga
    10921 ggaatcttta caattcacgc ccgttttttc taagaggagc gcaacgtgga aaggtttgat
    10981 gccattatta taggcgctgg tgcggcgggt atgttctgtt ctgcgctggc aggtcaggta
    11041 ggacgccggg ttctgctgat cgataatggt aaaatactag ggcgcaaaat ccttatgtct
    11101 ggcggtgggc gctgcaactt taccaacctt tatgtcgaac caggcgctta tctgagccag
    11161 aatccgcatt tttgtaagtc tgcactcgca cgttttaccc agtgggattt cattgatctg
    11221 gtcaataaac acggtatcgc ctggcacgag aaaacgttag ggcaactctt ctgcgatgac
    11281 tccgcgcagc agattgtcga catgctggtg gatgagtgcg agaagggcaa tgtaaccttc
    11341 agattgcgta gcgaagtgct gagtgtggcg aaggatgaaa caggcttcac gcttgatctg
    11401 aacggcatga ctgtcggttg cgaaaagctg gtcatcgcga ccggtgggct gtcaatgccg
    11461 gggctgggcg catcgccgtt tggttataag attgccgaac aatttggcct caacgtgctg
    11521 ccgacccgcg cgggtctggt gccattcact ctgcataaac cgttgctcga agagttacag
    11581 gtgctggcgg gcgtggcggt gccttccgtg attaccgctg aaaacggcac cgttttccgt
    11641 gagaacttac tcttcaccca ccgcggctta tctggaccgg cggtgttgca gatttcaagc
    11701 tactggcaac cgggtgaatt tgtcagtatc aatctgctac cggatgtgga cctcgaaacc
    11761 ttcctgaatg agcagcgtaa cgcacatccg aatcaaagcc tgaaaaacac actggcggtt
    11821 catctaccga agcggttggt tgaacgctta cagcaactcg ggcaaatccc ggatgtttcg
    11881 ctaaaacagc tcaacgtgtg tgaccaacag gcactgatta gcacattgac cgactggcgc
    11941 gtacaaccca acggcactga aggctatcgc actgccgaag tgacgctcgg cggcgtggac
    12001 accaacgaac tttcttcacg gacgatggaa gcgcgcaaag tgcctgggct gtacttcatc
    12061 ggtgaagtga tggacgttac cggctggctg gggggctata acttccagtg ggcgtggtcg
    12121 agtgcatggg cttgtgcgca ggatttggcg tcaattgtga aataaccatg acgttttccc
    12181 gtcagccgtt tgtatatgtc gttattaagt cattttggta cggttctgca tccgacattc
    12241 tggtgttaac tcgctgtttt gttagcgaat taacgctatt gccgttgtgg ttatt
//
LOCUS       DAZPAB010000108        12185 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZPAB010000108 DAZPAB010000000
VERSION     DAZPAB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.108"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..602)
                     /locus_tag="ACHUPG_002674"
     CDS             complement(<1..602)
                     /locus_tag="ACHUPG_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR0862227.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            1122..2021
                     /locus_tag="ACHUPG_002675"
     CDS             1122..2021
                     /locus_tag="ACHUPG_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0862228.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            2070..3083
                     /gene="yhjD"
                     /locus_tag="ACHUPG_002676"
     CDS             2070..3083
                     /gene="yhjD"
                     /locus_tag="ACHUPG_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR0862229.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            3494..4816
                     /locus_tag="ACHUPG_002677"
     CDS             3494..4816
                     /locus_tag="ACHUPG_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0862230.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(4998..7058)
                     /locus_tag="ACHUPG_002678"
     CDS             complement(4998..7058)
                     /locus_tag="ACHUPG_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR0862231.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(7128..7895)
                     /gene="pdeH"
                     /locus_tag="ACHUPG_002679"
     CDS             complement(7128..7895)
                     /gene="pdeH"
                     /locus_tag="ACHUPG_002679"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR0862232.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            8127..9056
                     /gene="kdgK"
                     /locus_tag="ACHUPG_002680"
     CDS             8127..9056
                     /gene="kdgK"
                     /locus_tag="ACHUPG_002680"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR0862233.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(9152..10648)
                     /locus_tag="ACHUPG_002681"
     CDS             complement(9152..10648)
                     /locus_tag="ACHUPG_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR0862234.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(10869..12155)
                     /gene="dctA"
                     /locus_tag="ACHUPG_002682"
     CDS             complement(10869..12155)
                     /gene="dctA"
                     /locus_tag="ACHUPG_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR0862235.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
BASE COUNT         2920 a         3310 c         3000 g         2955 t
ORIGIN      
        1 caggaggaga tatttaacat cattgccgcc tgggtgcgat ttttcacttc cagacggcga
       61 tacagggatt ccagatgcgc ttttaccgtt ccggtactga tattcagcgc tctgccgatc
      121 tccttatttg attcgcccgc cgctaacatg gttaaaatct cccgctggcg ggcgcttaac
      181 gatttgagat ctttaatgtc cttttccggc gtcgtccgcc agtctccagg cagaaacatc
      241 atccccatcg ccgcactatt taccgccaac gcaaatgtct cgacggttga atcgcgaggc
      301 acaatggcca gcacattaaa atggataact tcctgtaacc accgtttatt gcaatccgtc
      361 gccgtgatta acaccttaac ctcaggaaac tgcaccacgg ttttttgcag caaccagtag
      421 caaaactcac catcctgatc gccatcgagc ataactaagg cttcagggta actttccagc
      481 ttttgccata actcgtctgc ctgactgacc ccctgaatac tcactcctgg aatacgctgc
      541 tgtaaactga tttttattcc atgaataaat attgactgcc tgtcaaacat gactatttgc
      601 ataactgaat ctccacctga atacgttaaa aagacttaag tagtggaagg gtattacccg
      661 cgagaaaaaa taagaattcg ccatttggcg gtggccattc tacagagatg acgcgtagaa
      721 aatagttacc gatataaata gttacagcta aacgcctgaa attacatgtc gagggcacta
      781 tttaaaacaa ttttgaggat ttccttatat tggtggttag tacgcatgca attaaaaatg
      841 aaattccgcg actacaagcc aaaataacaa atggcaatga gacaaaaata agcacaaata
      901 gccaatacgt cctctattca ctttaaaggg aatcactgaa aaatacgctc tgtttaaggg
      961 gattcacctt tctcataaag ctattccgcc cttttcctgc tgagaaatcg ccacattcgt
     1021 catgacaaca ttgtgaaacc cggcattaga tgttagaaaa aacaataaac aatgcgatat
     1081 gcgcgttact gtttcgctgt gaacaacaat cggtcaaaga aatggataaa attcacgcaa
     1141 tgcagttgtt catcaaagtc gcggagctgg aaagtttttc ccgcgcggcg gatttctttg
     1201 ctttgccaaa gggaagtgtt tcgcgccaga tacaggcact ggaacatcaa cttggcaccc
     1261 agcttctcca gcgcactacg cgacgggtca aactcacgcc agaaggcatg acctattatc
     1321 aacgggcaaa agatgtgttg agtaatctca gcgaactgga cggtctgttt caacaggatg
     1381 ccaccagtat cagcggtaaa ttacgcatcg acatcccgtc aggaatcgcg aaaaacctgt
     1441 tactgccgcg tctgtcggaa tttctctatc agcatccggg aattgagctg gaactgagta
     1501 gccataaccg tccggtagat attcttcatg atggttttga ttgcgtgata cgcactggcg
     1561 cgttaccgga agatggcgtt atcgcccgtc ccctcggcaa actaaccatg gtcaactgtg
     1621 ccagtccgca ctatctgacg cgctttggtt atccgcaaag ccccgatgat ctgacttcac
     1681 acgcaatagt gcgttataca ccgcacctgg gtgtacatcc gttaggtttt gaggttgcca
     1741 gcgttaatgg cgtccagtgg tttaagtctg gcggcatgtt gacggtaaac agtagcgaaa
     1801 actatctcgc cgccggtctt gccggtctgg ggattattca gatcccgcgc attgccgtgc
     1861 gcgaagccct gcgtgccggg cggcttattg aagtgttgcc aggctaccgt gccgagccgc
     1921 tctccctttc gctggtttac ccgcagcgtc gggagctttc ccggcgtgta aacctgttta
     1981 tgcagtggct ggctggcgta atgaaagagc acctggactg accgactata ctttttaaga
     2041 atgaccacga cagaaggaca aaagagcgga tgacgcagga aaacgagatc aaacgtccca
     2101 cccaggatct ggagcacgag ccgattaagc agttggataa tagcgagaaa ggcggcaaag
     2161 ttagccaggc gctggaaacc gtcaccacca ctgccgaaaa agtccaacgc caaccggtta
     2221 ttgcgcacct tattcgcgcg acagaacgct ttaacgatcg gctgggtaac cagtttggtg
     2281 cggctatcac ctatttctca tttttgtcga tgatcccgat tttgatggtg tcgtttgccg
     2341 cagggggctt tgtgctggcc tcccatccga tgttgctaca ggatatcttc gacaaaattc
     2401 tgcaaaacat cagcgatccg acactagccg ccacgttgaa aaacaccatc aacaccgccg
     2461 ttcagcagcg tacgactgta gggcttgtcg gcctggcggt ggcactttat tccggcatca
     2521 actggatggg taacctgcgt gaagcgattc gtgcccagtc gcgcgatgtc tgggaacgct
     2581 cgccgcaaga tcaggagaag ttctgggtaa aatatctgcg tgattttatt tcactgattg
     2641 gtttgttgat tgcgctgatt gtgacgcttt cgatcacctc ggttgccggt tcggcgcagc
     2701 aaatgattat tagcgccctg cacctgaaca gcattgagtg gctgaaaccg acgtggcgat
     2761 tgattggcct ggcgatttcc atcttcgcca actatctgct tttcttctgg atcttctggc
     2821 ggttgccgcg ccaccgtccg cgcaaaaaag ccctgattcg cggaacattt ctggctgcta
     2881 ttggttttga agtgattaaa atcgtgatga cctacaccct gccatcgctg atgaaatccc
     2941 cctctggcgc agcatttggt tccgtgctgg ggctgatggc atttttctac ttcttcgccc
     3001 gtttgacgct gttttgcgcg gcgtggattg ccaccgccga atataaagac gacccgcgaa
     3061 tgccagggaa aacgcagcct taaaataggc cggatgcggc gtaaacgcct tatccggcat
     3121 acgattttat tcggcctggc tccccgtagg ccggataaga tgcgccagca tcgcatccgg
     3181 ctataatgcg cacataacct cttgaaactc ttccccagag cctcttcagc catctatttg
     3241 ggagcaaaca atttcactcc aactcataac cccagcatat aaatccagtt ggtaactttt
     3301 atttaacctg aaaccagttt tatccactat ttataaaatt atgtgaagca tttcatagaa
     3361 gaaaaatcac tggcctaaac attatcccct ttttgcctgg tttttgacca tttccgcgat
     3421 ttgttacaca ttgaaatatc acttttgctg tgcgtaatat ggctattcgt tagccaaaaa
     3481 ataagaaaag attatgcaag caacagccac aacactcgac cacgaacaag aatacacgcc
     3541 gatcaactcg cgtaataaag tccttgtcgc ctctctcatt ggcacggcca ttgagttctt
     3601 cgacttttac atttacgcca ctgcggccgt tattgtgttt ccgcatatct tcttcccgca
     3661 gggcgatcca acggcagcaa cgctacagtc gctcgccacc ttcgccatcg ccttcgtcgc
     3721 gcgccccatt ggctctgccg tttttggtca ttttggcgat cgcgttgggc gtaaagcgac
     3781 gctggtcgcc tcgttgctaa cgatggggat ttcgaccgtg gtgattggtc tgctgccggg
     3841 ctatgccacg attggtattt tcgccccact gctgctggcg ctggctcgat ttggtcaggg
     3901 tctgggctta ggcggtgaat ggggcggcgc ggcgctgctg gcgactgaaa acgccccacc
     3961 gcgcaaacgt gcactgtatg gctccttccc acagctaggc gcaccgattg gcttcttctt
     4021 tgccaacggc actttcttgc tgctttcctg gctactgacc gatgagcagt ttatgagctg
     4081 gggctggcgt gtgccattta tcttctcggc ggtgctggtc attatcggcc tgtatgttcg
     4141 cgtgtcgctg catgagtcgc cggtgtttga gaaagtcgct aaagcgaaaa aacaggtgaa
     4201 gatcccgctg ggtacgctgc tgaccaaaca tgttcgcgta accgtactgg gtacgttcat
     4261 tatgctggca acctatacgc tgttttacat catgacggtc tactctatga ccttcagtac
     4321 cgccgccgcg ccagttgggc ttggcctgcc gcgtaacgaa gtgttgtgga tgctgatgat
     4381 ggcagttatt ggttttggcg tgatggtgcc agtcgccgga ttactggctg atgcctttgg
     4441 tcgccgtaaa agcatggtga tcatcaccac gctgatcatc ctgttcgcgc tgttcgcctt
     4501 taacccgctg ctcggttctg gcaacccgat tctggttttt gccttcctgc tgctggggtt
     4561 aagtctgatg gggctgacct tcgggccgat gggtgcgctg ttaccagagc tgtttccgac
     4621 agaagtgcgt tacaccggag catcgttctc ttacaacgta tcgtcgattc tcggggcttc
     4681 cgttgcgccg tatatcgcag cctggttgca ggctaactac gggttaggtg cggtggggtt
     4741 atatctggcg gcgatggctg gcttgacgtt aatcgccctg ctgctgaccc atgagacgcg
     4801 acatcagtcg ttgtaataag tgtgaaaagc ctgatgcgct acgcttatca ggactgaatt
     4861 tccatacaat atattgaatt cacaaggatt tgtaggccgg ataaggcgtt cacgccgcat
     4921 ccggcatgaa caaagcacac tttgccaaaa atctaaatct gtttaattgc cggatgtcag
     4981 acatccggca attatcatca cttcttcatc tgcgacaata tcgtccgaca ctgattagcc
     5041 tccccttcgg aaggtgagat cagtgccagt aacgccgccg ccggtgttac cagcgttgcc
     5101 agtgccgcag caacagcacc acgggcaatc agcggtccgg ctttcacccc agcctgcgga
     5161 tttttaaacg tcccacgcac atacagcggc gaacgcagtg tgataatccg aatcccttta
     5221 ctctccggat caatagtcaa atccagctgt tccgaagcaa aacttgccgt gccggtaaca
     5281 ttaatcaacg cgttctcagt atcgaaagca aaaatctgcg ggcgcgccac gccgttggca
     5341 atattcagat tcgccgccgc gcagttcacc cgcacctcat cgtcaccaaa tatcgcaccg
     5401 acaatgtagt tgccgacatt cagcccaaca atctccatca ggttgcggct caccagcccg
     5461 tcattcatca acagtttcag gttgccgtta ctgttgccta aaagcgccgc caccgagtta
     5521 ccgctaccgc gtagttccgc gtcaccgttc atttccccca gcgtcttctg catcagttcc
     5581 acatcgggca tcagttcttt cagtttcagt cgacgagcct gaatatctgc ccgcccctgc
     5641 atcggctttt tatcgccttc cagatgaata ttcgccgcaa tgctgccgcc cgccatgcca
     5701 aatttcagcg gttgcaggcg caggtcagca tttttgagga tgatatgagt agaaagatcg
     5761 ctaatcggca ggctactgcc atgctcaatg cgccgccctt tgaagcgaac atcggcatcc
     5821 ataacgtccc atttatcggt ttcgaagcgg tcataaggca gcactttgcc cgcaggctga
     5881 acgctttttt cgcccttctt ctgttcagac cgtttcgact tttctgcccc tttcccggaa
     5941 tcaacgccaa tcaacggtcc caggtccgcc agccgcaatt gccgcgactc gacatcacct
     6001 tccagttttg gtcgtggctt tccggtggtg tagaccagag aaccgtggat atcgctatcg
     6061 ccaattcgcc cattaaaacc gcgataatca aagaccgacg atttttcagt gtcgattttc
     6121 gctaccagcc gaccatccgt ttcaaacggc ggggtatcgg gcagcagaac gcccgtcagt
     6181 tcatagagat cacccagtga atcgccagaa aatttaagcc gtaaatcgac accgcccatc
     6241 ttcattgggt cattcacgac gccatcaaaa gcaacacggg tattacctga acggaaatca
     6301 gcctgtaccg gaaacggcgt cccttcgcca cgcagcgcca gcataccgcc tatttttccc
     6361 gtacccgtga gcggttcacc gttataacgt ccctgcgcct tcaggccaaa aatgtaatcg
     6421 cccacctttt ctttatccgc tttacctttc gatccagtaa cttcgctgaa tggcagcggc
     6481 ttgcctaacg gatcaacaaa aatttccaga tccgctttgc ttactttgtc atcaatggcg
     6541 atccgccctt gatcgaaaag aatattatcc agccgaaacg accatgccga cggctttgca
     6601 ttcgcgtctt tgttgtcatc gttggcaaga ttaaacgtcc agttattgtt cttttcagag
     6661 aggcgaatca ggcgcgcgtc gggcttttcg agcttgatcc acggcagcca gaccgttttg
     6721 gtcagcagcg ccagcggtgc cagcgttgcc tctactcgcg gcaaatgcac catcgtgact
     6781 tcgggaatat ccggtggatt accaagaatg atgtcttctg catgtacatg aggccacggc
     6841 acccagctgc gccagccagt ttcttgtttt tgccgctccc acaccacgcc cagattgcca
     6901 cggatagcga acggacgatt caactccgca gagactttct ggttgatggt cggtttgagt
     6961 cgattccagt caaaagttgc aatcaaaatg atcgccacga caatcaacaa caagaaagcc
     7021 cctgaaatcg cagcggttat tttgcctgcc ttgctcatcg ttcgcttcct catattcttc
     7081 ctgtgccagt cctaaagata gtccagcccg gcggaaaatg aggcagctta tagcgccaga
     7141 accgccgtat tcagcgtttc catcggtgcc gggcgtgaaa gaaaccagcc ttgtgcggcg
     7201 aatgcgggcg agttctgaac atcacgccac tcttccggtg tttctacgcc ctcgacaatc
     7261 accccgcgac aatagcgatt cattagatgt aaaagctgag aaaagagtgt gcggccttcc
     7321 ggcgactgac gcagcatcac aaacagttct cgcgcgattt tgatgtagtc ataacgcact
     7381 tcacttagtg cagagaaatt tgccatcccg gtaccaaaat catccagcca cagcgggcca
     7441 aattcacaca tcgaggcaaa ggttgaatct ttcggcagac ggatatgctc caccagttcg
     7501 aaacgcagcc agggaagacg ctcaatctgg cgcaggattt ttggttgctg acgcagggcg
     7561 atgagcgtag ggccatcaat attgaccgat gccagcaggc cgtgctctac aaagaagtcg
     7621 gctttttgcg ccagcaaatc aatctgctct ttcacaacct ccatccgatg gctgacggtg
     7681 atttcagtaa aatagcgatc cggcggcagg cgttgcgaag ggttcaaggg atgcgtgacc
     7741 accgttaata gctccacggc cattaaccgc ccgcatgttt gatagatcgg ctgccaggtg
     7801 taagcacgct cacactgcaa ccaaaaacgc cgttcctgca agctctcgat gcttgcttca
     7861 gggttgctta ttcgctggat aacctgcctt atcatcaaag atgtcctgtg tttaaaagtg
     7921 atgctgcccg gactcgacaa agattatcgg cgcgtaaggg cagaacttta ccgcctggcc
     7981 ggggtaaatt tcctcactcc acaacggaat ctttcaggaa tacgttctgg ctcaaaaaaa
     8041 taatggaaca ctgttttaat atggttgacc agcaaaccac cacagcgcaa actaacgcta
     8101 attttttaca gatcaggttc acgactatgt ccaaaaagat tgccgtgatt ggcgaatgca
     8161 tgattgagct ttccgagaaa ggcgcggacg ttaagcgcgg tttcggcggc gataccctga
     8221 acacttccgt ctatatcgcc cgtcaggtcg atcctgcggc attaaccgtt cattacgtaa
     8281 cggcgctggg aacggacagt tttagccagc agatgctgga cgcctggcac ggcgagaacg
     8341 ttgatacttc cctgacccaa cggatggaaa accgtcttcc gggcctttac tacattgaaa
     8401 ccgacagcac cggcgaacgt acgttctact actggcgtaa cgaagccgcc gccaaattct
     8461 ggctggcgag cgagcagtct gcggcgattt gcgaagagct ggcgaatttc gattatctct
     8521 acctgagcgg gattagcctg gcgatcttaa gcccgaccag ccgcgaaaag ctgctttccc
     8581 tgctgcgcga atgccgcgcc aaaggcggaa aagtgatttt cgacaataac tatcgtccgc
     8641 gcctgtgggc cagcaaagaa gagacacagc aggtgtacca gcaaatgctg gaatgcacgg
     8701 atatcgcctt cctgacgctg gacgacgaag acgcgctgtg gggtcaacag ccggtggaag
     8761 acgtcattgc gcgcacccat aacgcgggcg tgaaagaagt ggtggtgaaa cgcggggcgg
     8821 attcttgcct ggtgtccatt gctggtgaag cgttagttga tgttccggcg gtgaaactgc
     8881 cgaaagaaaa agtgatcgat accaccgcag ctggcgactc tttcagcgcc ggttatctgg
     8941 cggtacgttt gacgggcggc agtgcggaaa acgcggcgaa acgcgggcac ctgaccgcaa
     9001 gtaccgttat tcagtatcgc ggcgcgatta tcccgcgtga ggcgatgcca gcgtaaacgc
     9061 agcatatgca aaaggctgga taaagtgtta cccctctatc cagctcatcc ataaatacct
     9121 gatggttgtg ctaactcatc accatcaggc attactgtgc aggtggaata tccgtcactt
     9181 caggatgtac gtcatccgtg gcgacagagg tggtcgcagc ggcggtagat ggggccatga
     9241 tttgatccca ggccgcctgc aacgctttca tgttaaattc cggctcgcct ttcggctgca
     9301 gcagtattaa cgccatatca ttcgacaact gctgacgcag atcctgattt aacatctcca
     9361 cagtcaggct gttaaggaaa tcctgccgca gtttctgata ctgctccggc gcgatatcga
     9421 cgacctgatt ttgcaacgaa cgcatccgct ggcccatcag aatatcggta tcagcccgtg
     9481 cataggcggc aaacagtttc tgcagctcca gtttcttttg cgccactaac gcattgaact
     9541 cttcttccgg cagaccttta tcgcgaacct tcgccagttc acgcgccacc agattaaggt
     9601 tgctgttcag cttgtcgttt ggtgattcga tgttgatggc acactgcgca cgcagataca
     9661 gcacacggca gtcaaatcca agaccgatgt ctttgctgtc actggcgctt aacgcttgct
     9721 gaacatgcca gaacagcgcc tcgcgggcca ggtccgcacg ccagtagcgc agcagtgcgg
     9781 ctgattcacg aatcggctgc cacggcgtat cccacatgat agataaccgg tcctgacgca
     9841 ccgcgtcagt cataatactc accgcttccg cacgcagcgg agaaagcgtc ggcaccggag
     9901 ctggcgtttc acgtttgcct ttcagttcgc caaacgtttt gttgatttgg tcgacaaccg
     9961 agcgcgcatc cacgtttccc accaccagta gcgtcattgc atccggggtg taccatttct
    10021 gatagaaatc tttaattttt tccgcttcaa cgggttgttt cagcggatcg gcaggatcgt
    10081 gacctaacaa ggttgaccct ttcagacgat agcgccacca gccctcttta gtatcggcag
    10141 gccaggttgc caccatgtcc tgactttgca gcgcgtggtt gatggtttct ggtgtgatgg
    10201 tcaatttgcc agtggcattt gccagataag agagcgcttc tttcagcaaa tcgttacggt
    10261 tattgggcaa actcagatta aacagcgtgg tgtcataaga gacaattacc ggcggcatcg
    10321 ggcgtttagg gtcgatcccc tgctgccaca atgaacgcgc ctgtgctgcg tcaaggccac
    10381 cgctttgcgt tagcgcaata cgagggatgg cgtgactgta accgctctgt tgtgtacttt
    10441 cggcgagcga accggtatta accagcaggc gaatttcaac acgatcgctg ggacgctggg
    10501 gggtggtcag cacttgccac tgtaaaccgt tggaaagcgt cccctgttgc catgctggat
    10561 caggctggag cgcatctgcc tgcacatagc cagcagtggc catcatcagc aaaccgcccg
    10621 ctaaaagtcg aatttttatg ccctgcatgt gaacccctga tcaactatcc tggtaataaa
    10681 aaaactgccc gttgtcggtc agtctaaatg acgttaaaaa cacttcgtgt tagaccgcaa
    10741 gaacatgaaa atgtcacgga agaagtgaaa taaacctgaa ctcacccagg tggtaagttc
    10801 aggcacaggg gtcaattatg cgcaaacacc cgcactcggg gaagggagtg cgggcataag
    10861 tggtgagatt aagaggataa ttcgtgcgtt ttgccatccg gcgcacgatt attcagcaca
    10921 tcgtccagtt ttttgtggtc cagttctttc acccacttag caacgacaat ggtcgctacg
    10981 ccgttaccga ccaggttagt tagcgcacga gcttctgaca taaagcggtc gataccgagg
    11041 atcagcgcca gacccgctac cggcaaatgg cccaccgcag agagcgtcgc cgccagcacg
    11101 ataaagccac tacccgttac ccctgccgcc cctttagaag aaagcagcaa cacgattaac
    11161 agcgtgattt ggtggacgat atccatctga ctgttagtgg cctgggcgat aaacaccgcc
    11221 gccattgtca ggtatatcga tgtgccatca aggttaaacg agtagcctgt cgggatgacc
    11281 agccccacca ccgatttacg gcagccgagt ttctccatct tgtcgagcat acgcggcagc
    11341 gccgactcgg aagatgaagt ccccagtaca atcagcagtt cttcacggat gtagcggata
    11401 aatttgaaga tactgaaacc agtcgcttta gcgattgaac ccaataccag caccacaaac
    11461 aggatacagg taatgtagaa acagataatc agctgcccca gttgcaccag tgtgccgacg
    11521 ccgtatttac cgatggtaaa cgccattgcc ccgagcgcac caataggtgc cagacgcatg
    11581 atcatattga tgatgccgaa gatgacctgc gagaaacttt cgatcacgtt aaaaatcagt
    11641 tggcctttgc tgcccagacg gtggagcgca aaaccaaaca gtacggcaaa cagcagcacc
    11701 tgcagaatgt taccgctggc aaatgcgcca atgacgctcg ccgggatgac atccataatg
    11761 aaggcgacaa tgccctggtc tttcgcctga tcggcgtaaa ccgctaccgc tttcgcatca
    11821 agcgttgccg gatcgacgtt cattccggca ccaggctgca cgacgttaac gatgataaga
    11881 ccaataatca gcgcgatggt actgacaatt tcaaagtaaa gcagtgcgac tgcgccggta
    11941 cgaccgaccg ccttcatgct ttccatgccc gcaatgcccg ttacgacggt acaaaagatg
    12001 acaggagcga tgatcatctt aatgagctta acgaagccgt cgccaagcgg tttcatttgc
    12061 tcgcctattt caggatagaa atggccaagg agaataccaa tggctatcgc tgtcaggacc
    12121 tgaaagtaaa ggcttttaaa cagagaggtt ttcatagggt gtcctttagt aaaaccacag
    12181 gtctt
//
LOCUS       DAZPAB010000109        12153 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZPAB010000109 DAZPAB010000000
VERSION     DAZPAB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.109"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            126..995
                     /locus_tag="ACHUPG_002683"
     CDS             126..995
                     /locus_tag="ACHUPG_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR0862236.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="ACHUPG_002684"
     CDS             complement(1198..1551)
                     /locus_tag="ACHUPG_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR0862237.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="ACHUPG_002685"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="ACHUPG_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR0862238.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="ACHUPG_002686"
     CDS             complement(3379..3672)
                     /locus_tag="ACHUPG_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR0862239.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="ACHUPG_002687"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="ACHUPG_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR0862240.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /locus_tag="ACHUPG_002688"
     CDS             complement(5220..5696)
                     /locus_tag="ACHUPG_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR0862241.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="ACHUPG_002689"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="ACHUPG_002689"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR0862242.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /gene="dcuA"
                     /locus_tag="ACHUPG_002690"
     CDS             7587..8888
                     /gene="dcuA"
                     /locus_tag="ACHUPG_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR0862243.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="ACHUPG_002691"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="ACHUPG_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR0862244.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /gene="dsbD"
                     /locus_tag="ACHUPG_002692"
     CDS             9318..11015
                     /gene="dsbD"
                     /locus_tag="ACHUPG_002692"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR0862245.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="ACHUPG_002693"
     CDS             11052..11627
                     /locus_tag="ACHUPG_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0862246.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11734..11809
                     /locus_tag="ACHUPG_002694"
     tRNA            11734..11809
                     /locus_tag="ACHUPG_002694"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11767..11769,aa:Phe,seq:gaa)
BASE COUNT         2932 a         3140 c         2980 g         3101 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcta tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc acgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctag cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggccgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 agatgtgcag attacggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag
    11761 gggattgaaa atccccgtgt ccttggttcg attccgagtc cgggcaccaa attcatatca
    11821 acggacctcc acggaggtcc gtttttcgtt tcaggacgcc acgatttaag cgttctgcct
    11881 ccaaatcaat tctaccgaac tcaaccagat tctccccaca tcaccagcaa tttgcgagca
    11941 tatcccaatt tgggaaaatt tgttcctgag ctatagcact aactgacgtg aaatgtcgtg
    12001 cggccccgtg atgctgttga acgtcaaatg acgtcatcag gagcgtaacg tatccataaa
    12061 gcacaacacc ggtctgaacg ccgactgatg agattttctg aatgagaaca aagagaaatg
    12121 tgtcagtccg cttgctcatg caaagactaa caa
//
LOCUS       DAZPAB010000110        12113 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZPAB010000110 DAZPAB010000000
VERSION     DAZPAB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.110"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..370
                     /locus_tag="ACHUPG_002695"
     CDS             <1..370
                     /locus_tag="ACHUPG_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862247.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="ACHUPG_002696"
     CDS             complement(458..1102)
                     /gene="cheZ"
                     /locus_tag="ACHUPG_002696"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR0862248.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="ACHUPG_002697"
     CDS             complement(1113..1502)
                     /gene="cheY"
                     /locus_tag="ACHUPG_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR0862249.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="ACHUPG_002698"
     CDS             complement(1517..2566)
                     /gene="cheB"
                     /locus_tag="ACHUPG_002698"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR0862250.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="ACHUPG_002699"
     CDS             complement(2569..3429)
                     /gene="cheR"
                     /locus_tag="ACHUPG_002699"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR0862251.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3448..5049)
                     /gene="tap"
                     /locus_tag="ACHUPG_002700"
     CDS             complement(3448..5049)
                     /gene="tap"
                     /locus_tag="ACHUPG_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR0862252.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5095..6756)
                     /gene="tar"
                     /locus_tag="ACHUPG_002701"
     CDS             complement(5095..6756)
                     /gene="tar"
                     /locus_tag="ACHUPG_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFR0862253.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6901..7404)
                     /gene="cheW"
                     /locus_tag="ACHUPG_002702"
     CDS             complement(6901..7404)
                     /gene="cheW"
                     /locus_tag="ACHUPG_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR0862254.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7425..9389)
                     /gene="cheA"
                     /locus_tag="ACHUPG_002703"
     CDS             complement(7425..9389)
                     /gene="cheA"
                     /locus_tag="ACHUPG_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR0862255.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9394..10320)
                     /gene="motB"
                     /locus_tag="ACHUPG_002704"
     CDS             complement(9394..10320)
                     /gene="motB"
                     /locus_tag="ACHUPG_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR0862256.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10317..11204)
                     /gene="motA"
                     /locus_tag="ACHUPG_002705"
     CDS             complement(10317..11204)
                     /gene="motA"
                     /locus_tag="ACHUPG_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR0862257.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11331..11909)
                     /gene="flhC"
                     /locus_tag="ACHUPG_002706"
     CDS             complement(11331..11909)
                     /gene="flhC"
                     /locus_tag="ACHUPG_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR0862258.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11912..>12113)
                     /locus_tag="ACHUPG_002707"
     CDS             complement(11912..>12113)
                     /locus_tag="ACHUPG_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR0862259.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2784 a         3388 c         2933 g         3008 t
ORIGIN      
        1 cactacgcta atgagttggg atgccgtgct ctatgatcta cgtagtgaaa aaattatcag
       61 taaagataat tacttgaccg acttaaaccg cagacatctt gaacttgttc ttcatactaa
      121 ccccaatcca agcggttcgg taacgaagat tttacgcact atttttacta agcaggtaaa
      181 gagtttaggg ccggaactct gtcatttctt gcaccatgaa ctgacgaaaa aacgttatga
      241 agtatggcat cactacgatg tactgcacta ccagtcatcg tcattttctc gttcacaatt
      301 taatgcattg ataaatttac tggcgcaaca atcacgcttc gctgatctaa aattatcaaa
      361 ctacaaataa ccatcctcca cgtactccct gaacgatacc cgccaccctg ctatcggctt
      421 ggcatcatcg cgcaggccga agttcaggca atacaaatca aaatccaaga ctatccaaca
      481 aatcgtccac ctgatcctga ctggctacca cgccggcttt gctggtatcg acctgaggtc
      541 cattaagcaa actctggttt tcacgttttg gacgcgactc ctgttccggg atgttttcca
      601 acagcaccat cagcaactgg cgttcgatct cctgaatgac atccatcatc cgcttaataa
      661 cctgcccggt gagatcctga aaatcctgcg ccatcatgat ttccagcagt tgcgcgttag
      721 taaagctggt atgcgcgggt acatctgcca gaaattgtcg tgtatctgtt accagttcac
      781 gggcgtcggc aaggtcaatc ggatcggcaa accagtcatc ccaacgttgg gttaacgctt
      841 ttgctgattt ctccatttga tcctgatgcg gttgtgacgc ctcaacactg ttcagcgccc
      901 gctccgcagc ctgggcggtc atctgcacaa catagtacaa acgatcgcgc gcatcgggga
      961 tggcttccgc cgcttcggca atggcctgaa ccagccccag ttcccgcaaa ctgtcgcgca
     1021 gcatatgcgt caggctgccg atgcgcgcaa tgatatcgcc agctgaatgc tcgtcagcag
     1081 gtttgattga tggttgcatc atagtcgcat cctcacatgc ccagtttctc aaagattttg
     1141 ttgagttttt cctccagcgt cgcggcggta aatggcttca ccacatagcc actggccccc
     1201 gcttgcgccg cagcaatgat gttctctttc ttcgcttctg cagtcaccat taacactggc
     1261 aatgccgaca tcgcgccatc cgcacgaatt gttttcagca attccaggcc atccatattg
     1321 ggcatgttcc agtcggagat aacaaatcca taaccgcctg cctgcaactt attgagagcg
     1381 tcgacaccat cttccgcttc ctcaacatta ttgaatccca gctctttcag caggttacgc
     1441 actatgcgtc gcatggtgga aaagtcatcc acaaccaaaa atttaagttc tttatccgcc
     1501 atttcacact cctgatttaa atacgtatcg cctgtccggc actaattgtt gccaacattt
     1561 gctggcttac ctggctaaga tcgaccactt cgcagacacc acccatattg atggcctcgc
     1621 gcggcatgcc gaacaccacg caacttgctt cgttttgcgc aagggtccat gcccccgcct
     1681 gacgcatcgc caacattccc gccgcgccgt cgttgcccat accggtcagg atcaccccaa
     1741 ccgcattacg ccccgcctgt ttggcgacag aatggaacaa cacatctacc gaaggccgat
     1801 gacggttaac cgccgggccg tcgtgaattt tgatttgata atttgcgcca ctacgcgcca
     1861 gttccatatg ccgatcgccc ggcgcaatat aggcatgccc cggcaagaca cgttctccgt
     1921 cttcggcttc tttaaccccg atctggcaaa gcttattaag tctgtcggca aaagagcggg
     1981 tgaaaccggg cggcatatgt tgggtaatta acaatgccgg gctggaaagc ggcaacggtt
     2041 gcagtacgtg acgaattgcc tcagttccac ccgttgaagc accaatcgca atcagttttt
     2101 cagaactcaa caacggccct gccttcagcg ttgtcggtgc tgacaatggc ttatgtgctg
     2161 caaggctcgc ctttgctgcc gtacgcacct tttcagcaat catttcgcta tacgccagca
     2221 ttccttcgcg aatacccagt tgcggtttgg tgacaaaatc tattgccccc agctccagcg
     2281 cgcgcagcgt gacttctgat cctttgccgg tcaaggaaga aaccatcaca acgggcattg
     2341 gacgcaaacg cattaatttt tcgaggaaat ccagtccgtc catccgcggc atttcaacat
     2401 ccagcgtcag cacatcggga ttgaatttct taatcaagtc acgcgcaacc agcggatcag
     2461 gcgcggtcgc caccatttcc atgtcgctat ggctgttgat gatttctgtc atgatctggc
     2521 gcatcagtgc cgaatcatcg acagataaca ccctgatttt gctcatcgtt aatccttact
     2581 tagcgcatac accgtctgac cacgcagcgt gaagcggcgc tcgaggtggc taaagttttc
     2641 agagtgaccc gcaaacagca atccgtcggg tttaaggagc ggaacaaagc ggcgcaaaat
     2701 ctcctgctgg gtagtttgat cgaagtagat catgacgtta cgacagaaga tcgcatcaaa
     2761 cggccccggc acggtgtact gtttcgccag taaattcagc ggggcaaaat caacatagtt
     2821 cgccagctcc tgacgcacgc gtaccagccc ttcatgcggt cccgtccctc gcatgaaata
     2881 ccgttgcagt tgctgcggcg tcaggttttt caactcttca tggcgataga taccgcttct
     2941 ggctttttcc agcacttcgg tgtcgatatc actggcaaac actttccagc gtccgggcac
     3001 ggtgcccaat gtgtcagcca gagtcatcgc aatgctgtac ggctcttcgc cggtcgaagc
     3061 cgccgcgctc catacgcgat cctcgccaga acggcgacgt gcgtgatccg cgagcagagg
     3121 gaaatgatga gcctcacgga aaaatgccgt cagattcgtg gtcagcgaat tgataaacgc
     3181 ctgccactca ccgctgtgct gattagattc cagcaagttc agataatgac cgaaatccgt
     3241 cagtcccaga gaacgcaaac gacgaaccag tcggttgtaa accatgtcgc gtttatggtc
     3301 agccagaacg atcccggctc gttgatagat caattgactt atccgccgaa aatgcgcgtc
     3361 ggaaagcgcc aggcgctcgg tcatctgtaa caataaagac gtttgcccac agggcagaga
     3421 tgaagtcata gcgccttctc aatcacttca ggataccact ggcgcaattt gtaactgcgc
     3481 cgactcatgt cgtgctactt catgttcttc aagggtaaat accgccacac gcgacgaaag
     3541 atggtcggcc tgattcgcca gttgttccgt tgccaccgcc gcttcttcta ccagcgagga
     3601 gttctgctga gtcacctgat ccatctggct gacagcctgt gcaacctgct caatcccccg
     3661 ccgttgttct tccgacgccg aagcaatttc tcccataatg tcgttcacgc gggtcaccga
     3721 actgacaata tcggtcatgg tcgcggcggc gttattcacc agtttcgaac cctgctggac
     3781 acgattgact gactcttcga tcagcccttt gatctctttt gctgcctgcg cgctacggct
     3841 ggcaagattg cgcacttcac ctgccactac cgcaaaacca cgcccctgct ctccggcgcg
     3901 agccgcttcc actgccgcat tcagggccag aatattggtc tggaaagcaa ttccgtcgat
     3961 aacgctgata atgtcgccaa ttttttgcga gctggtggcg atctcctgca tggtgtgagt
     4021 catggtactg acctgaacac cgcccgcctg cgccgttgtc gcggcatttt ttgccagttc
     4081 cgacgcctgt cgtgcgttat cggcgttttg ccctaccgtg gcggttaatt gctccatact
     4141 ggcggccgtt tgtgccagcg atgccgcctg ctgttcggta cgacttgaga gatcgttatt
     4201 gcctgcgaca atctccgcga tcccaatgtg catctcatgg cttcccttac gcacatcact
     4261 caccgtccca cgcaaagcct gctgcatggt cttcaggctg gcaaaaatgg cggtgatctc
     4321 attacgacca tataccgcaa tcggacgcgc cagattaccc gcagcaatgc tgtcaaaatg
     4381 gctaccgata atggccagtg gttgaacaat catcttgcgc gtccaccaca gcgcactgct
     4441 gatgtagatc gccgcaacaa taatcatgct gataaacacc agcgccgaaa tctgatagtt
     4501 acgctggctt tgcgcactgg cggcttccag cacatggttg atctccagct gccaggcctc
     4561 aaagttaacg tcaaacgcat tctgtgatcc ctgcaccggc gcagtgagga aatccgaaag
     4621 ttgattactt tcaagccagg tcgcctgatg ttcgagatcg ttgtgccagc gggcaaaact
     4681 tttctccgtc tctttttgca atgccctgac gtgctcgttg cccgcagtca tcgccataaa
     4741 acttttaaac agcgtggtgg attgcgtcag actggcgcgc gccgtcgtca tcaacgtttt
     4801 aatgtcatcc gccggatagc taagcgctgt cagagtgcct gctttgttca gcgcggtact
     4861 ggcctgtaac attaccgccc gagtttgcgc taatgccgca cgttgctgat tgctctgctc
     4921 gccgtgattc agtcgttgca aatcgtcgcg aaatgcccaa aaagacatgc cgttactgcc
     4981 aatctgcaag atcccgcaga gaatcaaaat taaaaacagc gtggtcgaaa ttcgaatacg
     5041 attaaacatc aacgctcccc atcaggcggc aatgaccgcg ttagtaaaaa ctcgtcaaaa
     5101 tgtttcccag tttggatctt gttcagtaat tcgcagtcgt ggctgtgccg gtggttgctc
     5161 actggcagga cgggatggtg tttgcggttt attggtgagt gggctggctg ccagacggaa
     5221 cgcggaaacc gcttgcgtta aacgactcgc ctgttcttcc agcgcagctg cggcggcagc
     5281 tgattcctgc accagcgatg cgttctgttg cgtgacgcga tccatttccg aaaccgccaa
     5341 tgcgacttga tcgatgccac ggctctgttc atccgatgcc gatgcaatct cgcccataat
     5401 gtcagtcacg cgagtgacag cattgacgat attgttcatt gtttccccgg cgctttcgac
     5461 cagcaccgaa ccggtatcaa tgcgcgagac ggagtcttca atgagggctt tgatctcttt
     5521 tgccgccagg gcgctgcgac tggcaagatt acgcacttca cccgccacca cggcaaaacc
     5581 acggccctgt tcacccgcac gcgcggcttc aaccgcggca ttcagcgcga ggatattagt
     5641 ctggaaggca ataccgtcga taacgctgat aatgtcggca attttcttcg aactatcggc
     5701 gatctcatgc atcgttttca ctacgccatc caccactttg ccgccgtgct gggcggtgtc
     5761 ggaggcactt tgcgccagtt gcgaggcctg gcgggcgtta tcggcgtttt gcttcactgt
     5821 cgcggtgagc tgctccatgc tggcggcagt ttcttccagc gcggatgcct gctgttcagt
     5881 acgggaggaa agatcggtgt tgcccgccgc aatttcacgg gtaccggcat agatggcatc
     5941 tgaaccttcg cggacatgag tgacggtgtc agtcaaagag cgttgcatat gtgaaacgct
     6001 ctgcgccagg tcacccattt cactgcgccc gtcaatggtc agggtattcg ccaggttacc
     6061 accggcgatt tcgcaaatgt gagcaataat ttttgccagc ggagtaagca acatacggcg
     6121 aatgccgtac cacgccacca gcagaatcaa taccaccacc agcgcgataa ccgccagttg
     6181 ccactgggca aatcggtaat catctgcgtt gtcagtgacg atatcgcgat acagtttttc
     6241 actgctgagg gcgtactgag caaacgcttc gcccattgca ttttgcattc cctgggttgg
     6301 ctgagcgaaa taagctccag tattgccata atcaagataa tcaatcagtt cagttaacgc
     6361 tgtgtaatag tttttatatt tttcatcaaa attacggctg gtagcgacca tttcaggtaa
     6421 cggtgccatg cttttgaatt ttttataatg cgtcgcggcc tgggccaatg ttttcctggc
     6481 gctatcgagc aattcaactt tggcgttact ttgttgattg gaggaatcca tcatcatccg
     6541 caccgctgaa cgactcaggt taatgcgcgt ttgcagcatt aaatcccagg ttgacgtcag
     6601 ctcgccctgc tgttcccgta attgattgga aaccacaaag ctcttctggc tatggtgaag
     6661 ggaagaaaaa aacaaactgc cggaaataag ctgtaacagt gcgaataccc ccagcaccat
     6721 taccaacagc gtgactacgc ggatacggtt aatcataagg caccttcctg aaaacaagtt
     6781 gatctcgtta tcggcaagaa ggggggaaac tttattgctg atgccacccg ccgcgaaatt
     6841 gaaataaaaa acccgatgcg cagatcatcg ggttcatttc aattgaggaa atcgggagaa
     6901 ttacgccact tctgacgccg cgctatctaa cagcgccatc tcttcgctgt tcagcagttt
     6961 ttcgatgttc accagaatca acatacggtc gcccagtgcg cccagtccag tgagatattc
     7021 tgttgaaagc gtcacggcaa actctggtgc cggacgaatt tgctccgccg tcaatgaaag
     7081 cacgtctgaa acgccgtcaa ccacgatgcc gaccacccgc tgtccgagat tcaggacgat
     7141 aactaccgtg ttgtcgttat agtccacatc cacctggctg aacttaattc gtaagtcaac
     7201 aatcggcaca ataacgccgc gcagattcgt gacgcctttg ataaacgctg gcgtgttcgc
     7261 aatccgtgtt acctgatcgt agccacggat ctcctgcact ttcaggatat caataccgta
     7321 ctcttcatca ccaagggtaa ataccagaaa ttcctggcct gacggctcgc tggccagctt
     7381 tgttacattc gtcataccgg tcatattgtt acctttttac tcattcaggc ggcggtgttc
     7441 gccatacgtt gttcgcggtt tatcgcctgc aaggcggaga catcaacaat cagtgccacg
     7501 ctgccgtcgc caagaatggt cgcagcagaa atgccgggga ctttgcgata gttactttca
     7561 aggtttttaa ccacaacctg gtgttgacca attaattgat ccaccagcaa ggcgtagcgg
     7621 cgaccgccac tttgtaagat caccacaatt ccctgggtgg cttcggtttt cgcgcccgcg
     7681 acgttgaaca ctttccacag ttcgacgatg ggcagatatt caccccgcac ttccagcacc
     7741 cgctcgccgc cggccagtgg atggagatcg gcttcacggg gttgcagtga ttccataaca
     7801 gcattcagcg gcagaatgaa aacttcatcc gcaacgcgta cggacatgcc gtcgaggatg
     7861 gccagcgtca gcggcagtaa aatgcggatc gtagtgccag taccctgctt cgactggatt
     7921 tcgacatgac cgcccatctc ctggatatta cgtttaacga cgtccatgcc gacgccgcgc
     7981 ccggagacgt cggtgacctg ctctgccgtg gagaagcctg gtgcaaatat cagcattgca
     8041 acttcgtcgt cgctcatgtt ttcgctgaca gtcaaacctt gcgaggccgc ttttgccaga
     8101 attcgctcac ggtttagccc cgccccatcg tcggtcactt caatgcaaat gttgccgccc
     8161 tgatgttcgg cagacagaat taaatttccg acgctgtttt tacctgcggc gagccgtttt
     8221 tctggcagtt caataccgtg atcgaggcta ttgcgtacca ggtgggtcag cgggtcgata
     8281 atgcgttcta tcaggctctt gtcgagctcg gtggaactgc ccaccagcgt cagttctacc
     8341 tgcttgccga gttttcccgc cagatcacgc accagccggg gatagcgact aaagacatat
     8401 tccatcggca tcatgcgaat cgacatcact gattcctgca aatcacgggc gttacgttgt
     8461 aactgcccca tgctggtaat caaatcacca tgattaaccg ggtccagttc gctggaacgc
     8521 tgggcaagca tggactgggt gataaccagc tcgccgacga ggttaattaa ttgatcaacc
     8581 ttttctaccg ctacacggat gctggtggat tcactgctgc gcgtcgtttt ttcccgctcc
     8641 acgcggccgg ttggcgcttg ttcggctgcc agtttaagca caggtggggt agctattttt
     8701 ggcgaaactt ctactgtttc aaaggtaatc tgatcggctt caatcacaaa acagagtacc
     8761 gctgtgatgt catcttcggc gatatcgccc ggtaatattg ccgagagaga atccgcccct
     8821 ttcaccacgt catttaacgt tgtcagatgc cccagctctt cttccagcag gtcgacttcg
     8881 ccggccttca ggcgcgaaag gataattcgt cgcggcaact gactgcgact ttgctcatct
     8941 tgcggttcac ttttggcaac cacacttaat cgggtcactg cggatggcgt ttcgcctttc
     9001 gcttctaatg ccagttgacg caaggcctgg cagatataat cgtagctggc ggcatccggc
     9061 tcttgcgact gtttataagc gtcgagctgt tcttgcatga tatccttcgt ttccaaaaac
     9121 agattgataa tgtcggtgtt gagttgcatc tcacctcgtc tggcttcatc gagcaggttt
     9181 tccatcagat gcgtggtttc ctgcaaaacg ctgaagccaa aagttcctgc ccctcctttg
     9241 atcgagtggg cagcccgaaa gatggcattc aattgttcgg catctggcgc ttccggctgc
     9301 aaaaccagca gatgctgctc catatcagcc aacagttcgt ccgcttcatc aaaaaatgtc
     9361 tgataaaaat cgcttatatc gatgctcacg ctgtcacctc ggttcggctg atggcattgt
     9421 gggaacactg acctgtggtg caacctcagg tttttctagg gcgcttactg gctcattctg
     9481 gctttcggcg ttttcatgca aaatggcctg ttcggcttgt ttgttcagta ccaacaggct
     9541 gatgcgacgg ttgacggcat catcaggtcc gcgatcgctt aagcgcatcg ttgccgccat
     9601 gccgacgaca cgtaacactt tgccgctatc caaccctccg accatcagtt cgcggcggga
     9661 tgcattggcc cgatcggcag aaagctccca gttgctgtaa cctttctcac cgctggcgta
     9721 gggaaaatca tcggtatgac ctgaaaggct aatacggttg ggaataccgt tcagcacagg
     9781 cgcgatggcg cgcagaatgt cgcgcatata gggttcgaca tcggcactgc cggttctaaa
     9841 catcgggcga ttctggctat cgatgatctg aatacgtaga ccttcctgga ccagatcgat
     9901 tttgagatgg agacgtaacg cccgcagttt cggatcggac tctatcaact ggtcgagatc
     9961 accccgcaat ttccgcaatc gactttgctc catgcgtttt ttcagctctt cgatgttcgg
    10021 ctgcttattc acttccccct ggctttgggt gtaatcatca ccaccgccgg gaattgggct
    10081 ttcactattg gaaatgcgat cgccgcccgt aaccgctgtc gccagtggag tccggaagta
    10141 ctccgcaatc tgaatcaact cttttgggct ggagatggag atcagccaca tcaccagaaa
    10201 aaaggccatc atcgcagtca taaagtcggc ataagcaatc ttccacgatc catgtgctgc
    10261 cccgtggctt ttggctttgc gtcgtttgac gacaataatc ggatgcgctt gattcttcat
    10321 gcttcctcgg ttgtcgtctg ttgttgcgga tttttcaccg cacgcacatg ctcttccagt
    10381 tcaataaacg acggacgttc gctggaatag agcgttttgc gaccaaactc aacggcgata
    10441 ggcggtgcgt aaccgttcag attagaaagc agagtgactt tgacgcactg catcattttg
    10501 ctggtttcgg cgcttttctg acgtaaaaca ctcgctagtg gggaaataaa tccgtaagcc
    10561 aataaaatac cgaggaaagt ccccaccatc gcatgtgcga taagcgcccc cagctcggcg
    10621 gcaggacgat cggctgaacc taacgcgtga acgcccccca ttacagccgc gacaatacca
    10681 aacgccggaa gtgagtcccc gaccagcgcc agactgttcg ccgggacttc tgcctcgctt
    10741 tcgtgcgtct caatctcttc atccatcagg gcttcgattt cgaaggtgtt catgtgaccg
    10801 ctgataatca ggcgcagata atcgacgata aaatcaagca tgacgctatc cgcgaggatg
    10861 cgtgggtagc tggcgaagat ctcgctctca cggggatttt caatatcacg ttccagcgaa
    10921 aacatcccca tctgccgcga tttcgccatc aaccgataaa gcagagccag cagatccata
    10981 tacattgctt tggtgtattt ggagcgacga aacagcaacg gcagcgcttt cagcgtgcct
    11041 ttaatcgctt tgccattatt gccgacgata aacgacccaa tccctgcacc ggcaataatc
    11101 accagttcag cgggttgata gagtgctcca aggcttccac cggtcatcaa ataaccgccg
    11161 aaaactgtac cgagaacaac caggtaacct aataagataa gcacgacatc atccttccac
    11221 tgttgaccat gacaggatgt tcagtcgtca ggcgttaacg cgcgattggg gaaaaaaaaa
    11281 gcagcggtac gtcgttaccg ctgctggaat gttgcgccac accgtatcag ttaaacagcc
    11341 tgtactctct gttcatccag cagttgtggg ataatatcgg caggattctg ggaaagttta
    11401 cgtcttttta ctgcccggga tggcggttga cataagctgc aggcaaagct gccaacaggc
    11461 tggtgagcgt gggtaataaa attgccgccg cagcagttgc agctggaaag ttgcagtaat
    11521 ccactttcaa caaaccgcac caatgtccag gcacgggtta atgccagcag tggtccttct
    11581 tctgcttgtg ggcactgttc aaggtataaa cggtaggctt tgatcaccgc atcgacgcca
    11641 ttacacaaac cggttttcag taaaaactgc catgcattac agaacatcga agcatgaacg
    11701 ttttgttccc aggtcataaa ccagtcggtt gagaatggca gcatgccttt cggcggtggg
    11761 cttccgcgca gttctttata aagttttatc aggcgtccgc gacttaactg tgtttcgctt
    11821 tccagcatct gcaaacgagc gcccagggtg atcaattcca ttgccagctg aatatcccgc
    11881 gcttcctgaa caatgctttt ttcactcatg atcaggccct tttcttgcgc agcgcttctt
    11941 caggctgatt aacatcattc agcaagcgtg ttgagagcat gatgccggta tgaatttgct
    12001 ggagatcgtc aacgcgggaa tcttgcgtca actgagtaat cgtctggtgg ctgtcaaaac
    12061 ggaagtgaca aaccagctga ttggtttctg ccagcttaac catttgcgga aga
//
LOCUS       DAZPAB010000111        12060 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZPAB010000111 DAZPAB010000000
VERSION     DAZPAB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12060
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.111"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            241..1011
                     /locus_tag="ACHUPG_002708"
     CDS             241..1011
                     /locus_tag="ACHUPG_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR0862260.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1002..1475
                     /locus_tag="ACHUPG_002709"
     CDS             1002..1475
                     /locus_tag="ACHUPG_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR0862261.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1582..2121
                     /gene="dnaT"
                     /locus_tag="ACHUPG_002710"
     CDS             1582..2121
                     /gene="dnaT"
                     /locus_tag="ACHUPG_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR0862262.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2124..2861
                     /gene="dnaC"
                     /locus_tag="ACHUPG_002711"
     CDS             2124..2861
                     /gene="dnaC"
                     /locus_tag="ACHUPG_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR0862263.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2910..3404
                     /gene="yjjA"
                     /locus_tag="ACHUPG_002712"
     CDS             2910..3404
                     /gene="yjjA"
                     /locus_tag="ACHUPG_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR0862264.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3644..5935
                     /gene="opgB"
                     /locus_tag="ACHUPG_002713"
     CDS             3644..5935
                     /gene="opgB"
                     /locus_tag="ACHUPG_002713"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR0862265.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6073..7095)
                     /gene="lgoD"
                     /locus_tag="ACHUPG_002714"
                     /pseudo
     CDS             complement(6073..7095)
                     /gene="lgoD"
                     /locus_tag="ACHUPG_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7234..8148
                     /locus_tag="ACHUPG_002715"
     CDS             7234..8148
                     /locus_tag="ACHUPG_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR0862266.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8363..9724
                     /locus_tag="ACHUPG_002716"
     CDS             8363..9724
                     /locus_tag="ACHUPG_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0862267.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9773..11437)
                     /gene="tsr"
                     /locus_tag="ACHUPG_002717"
     CDS             complement(9773..11437)
                     /gene="tsr"
                     /locus_tag="ACHUPG_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFR0862268.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11556..12002)
                     /gene="hpaR"
                     /locus_tag="ACHUPG_002718"
     CDS             complement(11556..12002)
                     /gene="hpaR"
                     /locus_tag="ACHUPG_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR0862269.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
BASE COUNT         2944 a         3182 c         2923 g         3011 t
ORIGIN      
        1 gctatctatt atcctgaccc gtaaaggcta aacaataaat tcattatagt gataatattc
       61 atgtgatagt ttgtttgcgg cgagagataa ttcgcttttt atcaccgagc ttgtcccaat
      121 aaccaggatt aatcagcgca attttgatct taacctaccg caaaacaacc gtgcttcatg
      181 atacaaatgg attctcatta tctgaacaat acgcagcacg tctatgacaa agggcgagtt
      241 atgcaaactg agcaacagcg agccgtaacg cggctttgta tccagtgtgg attatttctt
      301 ttgcaacatg gtgcggaaag cgcgttggtt gatgagcttt cctcacgact gggtcgggcg
      361 ctgggaatgg acagcgtcga aagttctatc tcttcgaacg ccatagtgct gacaactatt
      421 aaagatgggc aatgcctgac atcgacacgt aaaaatcacg atcgcggcat taatatgcat
      481 gtggtgactg aagtccagca cattgtgatt cttgcggagc atcatctgct ggattacaaa
      541 ggcgtagaga aacgatttag ccaaattcaa ccattacgtt acccaagatg gctggtagcc
      601 ttaatggttg gcctttcttg cgcctgtttc tgtaaactca ataacggtgg ctgggatggt
      661 gccgtcatca ccttctttgc cagtacgacc gcgatgtata tccgccagct gctggcacaa
      721 cgtcatcttc atccacagat caacttttgc cttaccgctt tcgccgccac caccatttcc
      781 ggattgcttt tgcaactccc cactttcagc aataccccca ccattgcgat ggccgccagc
      841 gttctgctgc tagtgccggg ctttccgttg attaatgccg tcgccgatat gtttaaaggc
      901 cacatcaata ccggactggc acgctgggcg atcgccagtc tgctgacact ggctacctgc
      961 gtcggcgtag tgatggcact gacgatttgg gggctacgcg gatgggtgtg atcgaatttc
     1021 tgttagcgtt ggcgcaggat atgatcctcg ccgccattcc tgcggtcggc tttgcgatgg
     1081 tgttcaacgt tcccgtgcgg gcgttacgct ggtgtgcgct gcttggctcg ataggtcatg
     1141 gttcccgaat gatcttgatg accagcgggt tgaatattga gtggtcaacc tttatggctt
     1201 ctatgctggt cggtaccatt ggtattcaat ggtcgcgctg gtatctggcg catccgaaag
     1261 tgtttaccgt ggcggccgtt atccctatgt tcccgggcat atcggcttat accgcaatga
     1321 tttcgtcggt aaaaatcagc cagttaggtt acagcgaacc gttgatgatt accctgttaa
     1381 ccaactttct tacagcttca tcgattgttg gtgcgttatc catcggtctt tccattcctg
     1441 gattatggtt gtaccgcaag cgccctcgcg tataaaattg cctcgcttaa gcacacggat
     1501 gagagacagc ctcctctcct ccgtgtgtta ctataaaagt tatctccctt ctcgttcatc
     1561 gttccatatt tgagaaacag tatgtcttcc agagttttga ccccggacgt cgttggtatt
     1621 gacgccctgg tacacgatca ccaaaccgtt ctggcaaaag ctgaaggcgg tgtggttgcc
     1681 gtatttgcta acaatgcccc ggcgttttat gccgtcacgc ctgcacgcct ggctgaactg
     1741 ctggcgctgg aagaaaagct agcgcgtccg ggaagcgatg tcgctctgga cgatcaactc
     1801 tatcaggaac cgcaagccgc tcccgttgct gtacccatgg ggaaattcgc catgtatccg
     1861 gactggcaac ccgatgccga ttttatccgc ctggcggcgc tatggggcgt ggcgctaaga
     1921 gagccggtga ccaccgaaga actggcctca ttcattgcct actggcaggc ggaaggtaaa
     1981 gtctttcacc atgtgcagtg gcaacaaaaa ctggcgcgca gcctgcaaat cggtcgtgcc
     2041 agcaacggcg gactgccgaa acgagatgtg aatacggtca gtgaacctga cagccaaatt
     2101 ccaccaggat tcagagggta acgatgaaaa acgttggcga cctgatgcaa cgcctgcaaa
     2161 aaatgatgcc tgcccatatc aaacctgcat tcaaaacggg tgaagagcta ctggcctggc
     2221 agaaagaaca aggggcgatc cgctccgccg ctctcgaacg tgaaaatcgg gcgatgaaaa
     2281 tgcagcgcac ctttaaccgc tccggtattc gtccactgca tcagaactgc tcctttgaga
     2341 actatcgcgt tgagtgtgaa gggcagatga atgcgttaag caaagcgcgc cagtatgtcg
     2401 aagagtttga cggcaacatc gccagcttca tcttttctgg taagccagga accggcaaaa
     2461 accatctggc ggcggcaatc tgcaacgagc tgctgctacg cggtaaatcc gtattgatca
     2521 tcaccgtggc cgatattatg tcggcgatga aagatacctt caggaatagc ggtaccagcg
     2581 aagaacaact gcttaacgat ctgagcaacg ttgatctgct ggtgatcgat gagatcggcg
     2641 tgcagaccga atcgaaatac gaaaaagtga tcatcaacca gatcgtcgat cgccgctctt
     2701 cttccaaacg cccaaccggg atgctgacca acagtaatat ggaagagatg accaagttgc
     2761 tgggcgaacg cgtgatggac cgtatgcgcc tgggtaacag tttatgggtg atcttcaact
     2821 gggatagcta ccgtagccgg gtaacgggta aagagtatta atccccgttt ctggcggtat
     2881 actgcgacac aattccacct tgagtaatga tgatgaaaac tattaaacat cttctgtgct
     2941 gtgccattgc cgccagcgca ttaatttcca ccggggtgca tgctgcgtcc tggaaagatg
     3001 cgctctccag cgccgccagc gaacttggca accaaaacag cacgacacag gaaggcggtt
     3061 ggtcgctcgc gtcattaact aacttgctta gcagcggaaa ccaagcctta agcgcagata
     3121 acatgaacaa tgccgcaggc attctgcaat actgcgcgaa gcaaaagctg gcttcggtaa
     3181 ccgatgccga aaacatcaag aaccaggtgc tggaaaagct gggcctgaac agtgaagagc
     3241 aaaaagaaga caccaactat ctggacggta ttcagggttt gctgaaaaca aaagatggtc
     3301 agcaactcaa tctggataac atcggaacga ctccgctggc agaaaaggtg aaaaccaaag
     3361 cctgcgatct ggtgttaaag caggggctga acttcatttc ctgaaccgcg aaatgttacc
     3421 cggctgacgc ggctctctcc tgcccttttc cctgctcctg gacggttttc cccctgttac
     3481 acgggaagcg accgcgatca aaaaagtctg tttataaact aattcctaac aacatcgcat
     3541 ttttttgaca ctagaattgc agcaatatgg cgacgccatt acattgtgct tctcaaaaag
     3601 cttaaacgtt ggctaaacgg cccggtttag tgaggatcgc ccattgtcag aactactctc
     3661 tttcgccctt tttctcgcct ctgtgctgat ttacgcatgg aaagcgggac gtaacacctg
     3721 gtggtttgca gccacgttaa cggtgctggg gctatttgtc gttttaaata tcaccctgtt
     3781 tgccagcgac tattttactg gcgatggtat taacgacgcg gttctctata ccttaaccaa
     3841 cagcctgacc ggtgctggcg tcagcaaata catcctgccg ggtatcggca ttgtgctggg
     3901 gctgacggcg gtgttcggtg cgctgggctg gatcctgcgc cgtcgtcgtc atcatccgca
     3961 ccattttggt tacagcctgc tggcgctctt actggcgctg ggttcggtgg acgccagccc
     4021 ggcatttcgt cagataacgg aactggtgaa atcccagtca cgcgatggcg acccggactt
     4081 tgcggcttat tataaagagc cgtcgaaaac tatccctgac ccgaaactca acctggttta
     4141 tatctacggc gaaagtctcg agcggaccta ttttgataac gaagctttcc cggatctcac
     4201 gcctgaactg ggcgcgttga aaaatgaagg cctggatttc agccacacgc agcagctgcc
     4261 aggaacggat tacacgattg cgggcatggt ggcttctcag tgcggcatac cgctgttcgc
     4321 cccctttgaa ggcaacgcct ccgcctctgt ctccagcttc ttcccgcaga acatctgtct
     4381 gggcgatatc ctgaaaaact cgggttatca gaactatttc gtgcagggcg caaatctgcg
     4441 ttttgccggt aaagatgtgt tcctgaaatc gcacggcttc gaccacttat acggctcaga
     4501 agagctgaaa agcgtggtag ccgacccgca ctatcgcaac gactggggat tctacgacga
     4561 taccgttctc gatgaagcgt ggaaaaagtt tgaagagctt tcccgctcag gtcagcgatt
     4621 ctcactgttt accctgacag tcgataccca tcacccggat ggttttatct cgcgcacctg
     4681 taaccgcaaa aaatatgatt ttgacggtaa gccgaatcag tcattcagcg cggtaagttg
     4741 cagccaggag aatatcgcga cgtttatcaa caaaatcaaa gcgtcaccgt ggtttaaaga
     4801 tactgtcatc gtcgtctctt ctgaccattt agcgatgaac aacacggcgt ggaaatacct
     4861 caataagcag gatcgcaata acctgttttt tgtcattcgt ggcgacaagc cgcagcaaaa
     4921 gacgctggca gtgaagcgta acacgatgga taacggcgcg acggtgctgg acattctcgg
     4981 tggcgataac tatctcggac ttggtcgtag cagtttatcc gggcagtcga tgtcggaaat
     5041 cttcctcaat atcaaagaga aaacattggc gtggaagcca gatatcatcc gcctgtggaa
     5101 attccctaaa gagatgaaag agttcaccat cgaccagcag aaaaacatga ttgccttctc
     5161 gggtagccat ttccgtttgc cgctgctgtt gcgggtttca gacaaacgcg tggaaccgct
     5221 gccggaaagc gaatactcag caccgctgcg tttccagctg gccgatttcg ctccacgcga
     5281 caatttcgtc tgggttgaca gttgctacaa gatggcacaa ctctgggctc cggaactggc
     5341 actttccacc gactggtgtg tctcgcaggg gcagcttggc ggtcagcaaa ttgttcagca
     5401 tgttgacaaa acaacatgga agagcaagac ggcatttaaa gatacggtca tcgacatggc
     5461 gcgttacaaa ggcaatgtcg atacgctgaa gattgttgat aacgatattc gctacaaagc
     5521 cgacagtttc atctttaacg tcgccggtgc gccagaagag gtgaaacagt ttagcgggat
     5581 ttcccgtccg gagtcgtggg gccgctggtc caacgcgcag ctgggcgatg aagtaaaaat
     5641 cgagtacaag catccgctgc cgaagaaatt tgacctggtg attaccgcca aagcatacgg
     5701 caataacgcc agccgtccta ttccggtacg cgtaggcaat gaagaacaaa ctcttgtgct
     5761 gggcaatgaa gtgaccacca ccacactgca tttcgataac ccaaccgatg ccgacacact
     5821 ggtaattgtg ccgccggaac ctgtctcaac caacgaaggg aatatcctcg gacactcgcc
     5881 gcgtaagctc gggatcggca tggtggaaat taaagtggta gaacgtgaag ggtaattcct
     5941 gactgatgtg taggtcgcat agggtgcgac ctacacgcct tctccggctt acaacacatt
     6001 attaagaaac aaaaaaaaca gcgcccaaca cttccccgga cgctgcatgg caactttaat
     6061 aatctcgcct tatcagaaag taattacacc cttaatcaac tcacgattgt taatcacatc
     6121 gcgctcgtag gtttctgcca gcgtggcgaa cggatagcga tgggttaaca tcatgtcggc
     6181 agtgattttc ccttccgcca tcagtcgacc gactttagcg aaatcttccg gcgtggcgtt
     6241 gcggctgccc atcatcgtcg tttctttttt atggaattcc ggatcggaga actgcaactc
     6301 acctttaaac agaccgacaa ataccaccgt gccgccgtga cgaatcagat tcacggtatt
     6361 attcatcgca tgttgattac ctgtcgcgtc gatcactttc tgcgccagcg aaccaccaaa
     6421 ctgcgcccgc agctgcgcgt caaaatcttc ggctgacggg tccagcacgg gcaactccag
     6481 acgcgttgcc acatgttcac ggcgcgccgg actggtatcc gccaccacca cctgtgcgcc
     6541 atcggcttta gcgattgccg ccgcgcccag accgattggc cccgccccga ccaccagcac
     6601 ctgctcgccg ggagcaatgg ctgcgcgacg caccgcatga gcgctaatag cgaaaggttc
     6661 aatcaatgcg gccgcctgcg ggtcaatacc gtctgcgggc aaaatgttcg ccaccggcac
     6721 gctcaaatac tcactaaaac cgccatcctg atgcacgcca atgactgaaa ttttttcaca
     6781 gcagttggta cgcccgcttt tacacgccgg gcattgctga caggcaacat aagggatcac
     6841 agcaacttgc tgaccatttt taagatcagc aatattttta cccagcccaa caatctcccc
     6901 acatatttca tggcctaaaa cccgtggata actaaaaaat ggttgatttc caccctaggc
     6961 atgaatatcg gtaccgcaaa tcccgacaga cttaattttt attaatgctt cattgtcacc
     7021 cggaataggt atctcgcgtt gtttccagac taattctttc ggctgctggc aaattaaaac
     7081 attcatcgta gacatgattc ggctatccat tttttgttgc cttagatatc aatgaaaatg
     7141 aaaaatggca gcggaacgga aaatctgttt tgtgaaaaca cacgcataaa aatgttttaa
     7201 atcgggtttt aatggactca caaggaggaa aatatgagtc gttcacaaaa tttacgccac
     7261 aatgtgatta accaggtcat tgatgatatg gcgcggggtc atatcccttc cccacttcca
     7321 tcgcaaagtg cgctggcgga aatgtacaac atcagccgca ctacagtgcg tcatattctc
     7381 agccaattac gcgaatgcgg cgtcttaacg caggttggaa acgactatgt tattgcgcgt
     7441 aaacctgacc atgatgacgg tttcgcctgt actaccgctt caatgagtga acaaaacaaa
     7501 gtgtttgagc aggctttttt cacgatgata aaccagcgtc agttacgccc tagggaaact
     7561 ttttctgaac tgcaactggc tcgggccgcg ggtgtcagcc cggtggttgt aagagaatat
     7621 cttttaaaat tcggccgtta taatcttatt caaagcgaaa agcgcggcca gtggagcatg
     7681 aagcaattcg atcaatccta cgccgagcag ctgtttgagc tacgcgaaat gctcgaaaca
     7741 cattcacttc agcactttct caacctgcct gatcatgatc cccgctggct gcaggcgaaa
     7801 accatgctag aacgtcatcg gctactgcgt gacaacatcg gcaatagctt tcgcatgttc
     7861 tcgcaacttg acagagattt tcactcctta ctgctttcag ctgccgacaa tatctttttt
     7921 gatcaatcgc ttgagatcat ctcagtcatc tttcattttc actatcaatg ggacgaaagc
     7981 gatcttaaac agcgcaatat cattgccgtc gatgaacata tgaccattct cagcgctctg
     8041 atttgccgca gtgaccttga tgccaccctt gcgctccgta accatttgaa ttcagctaaa
     8101 caatcaatga ttcgatccat taatgagaac acgcgttatg ctcattaagt acggattaaa
     8161 aacagcaaac tattagcggg tgcacggttt tatcaaaaga taaataccgg gaaattcccg
     8221 cttacctatg ctcacaatca gtcgactaaa aatgtttttg tgagcggtag taaagtccta
     8281 aaactttaac ctgccgttaa tgtcaacctt gcgtaatgca caattacgta aacaattaaa
     8341 attataaata caggagtccg gcgtggaaaa agaaaatatc accctcgatc cgcgttcttc
     8401 atttactcca tcttcgtcgg cagatattcc cgtgccacca gatggattag ttcaacgcag
     8461 tacccgaatt aaacgcattc aaaccaccgc catgttgtta ttattttttg cggcggtaat
     8521 caattatctc gaccgcagtt cgctgtcggt agcaaattta acgattcgtg aagaattggg
     8581 attaagtgcc accgaaatcg gcgctttgct ctccgtgttt tcactcgctt acggcattgc
     8641 gcaacttcct tgcggcccac tattggatcg taaaggccca cgcctgatgc tgggactggg
     8701 gatgttcttc tggtcactgt tccaggccat gtctggcatg gtgcacagct ttacgcagtt
     8761 cgtgttggtg cgtatcggta tggggattgg tgaagcgccg atgaacccat gcggtgtaaa
     8821 agtcattaac gactggttca acatcaaaga gcgcggacgc ccgatgggct tcttcaacgc
     8881 agcttctacc attggcgttg ctgtaagccc accgattctg gcggcaatga tgctggtgat
     8941 gggctggcgc gggatgttta ttaccattgg tgtactgggg atttttctcg ccatcggctg
     9001 gtatatgctc tatcgcaacc gcgagcacgt agaactgact gccgttgaac aagcttatct
     9061 caatgcaggt agcgtcaatg cccgccgaga tccgctcaat tttgccgaat ggcgcagcct
     9121 gttccgtaac cgcacaatgt ggggaatgat gctcggattc agtggcatca actacactgc
     9181 gtggctgtat ctggcctggc ttcctggtta cctgcaaaca gcctataacc tggatttaaa
     9241 aagcacaggg ttgatggcgg ctatcccttt cctgtttggg gctgccggga tgctggtcaa
     9301 cggttacgtt actgactggc tggtcaaagg gggaatggct ccgattaaaa gccgtaagat
     9361 ctgcattatt accgggatgt tctgttctgc cgcctttacg ctgatagtac cacaagcgac
     9421 aacatccatg atggcggttc tgctgattgg catggcactg ttctgtattc actttgccgg
     9481 aacatcctgc tggggcttga tccacgtcgc agttgcttct cgcatgactg cgtcggtggg
     9541 cagtatccag aactttgcca gcttcatctg cgcctctttc gcgccgatca ttactggttt
     9601 tattgttgat acaacccact cattccgtct ggcactaatc atttgcggtt gcgtcaccgc
     9661 agcgggagca ctggcgtaca tcttcctggt tcgccagccg atcaacgacc cgcgtaaaga
     9721 ttaaactccc tatgcccgat aacattttgc ttatcgggca tttttatggc gattaaaacg
     9781 tttcccagtt ctccccgcta tctgccactg ccattttacg cggcgtagct ggcgtcacgg
     9841 tttttaccac agccgatgtt tcacgctgct gttgctgctg ttgctgaatc cggaacactg
     9901 ccacagcttc ggtcaggcga ctggcctgct cttccagcgc ggcggcggca gcggcagatt
     9961 cttccaccag cgcggcgttc tgttgagtta cccggtccat ctcagcaacc gctaagccaa
    10021 cctgatcgat accacggctc tgctcatcag aagcagaagc tatttcgccc ataatgtccg
    10081 tcacgcgggt cacagcactg acaatctccg ccattgtttc cccggcgctt tcgaccagcg
    10141 tagagccaac atccactttc cccaccgagt cttcaatcag gcttttaatt tcacgaaccg
    10201 cctgggcgct acgctgggcc agattacgca cttctcccgc aaccaccgca aaaccacgtc
    10261 cttgctcacc cgcacgcgct gcttcaaccg ccgcgttcaa agccagaata ttggtctgga
    10321 aggtaatgcc gtcaattacg ctgataatat cggcgatttt ctgcgaactg gtggagatat
    10381 cgcgcatagt ctgcaccacg ttatctacca ctttgccgcc gcgttgcgcc gtttcagaag
    10441 cacttaatgc cagatggctg gcctggcgcg cattctcggc gttctgtttc accgttgcgg
    10501 tcagttgctc catgctggct gccgtctctt ccagcgaagc ggcctgttgc tcggtgcgcg
    10561 aagagagatc attattgccg gtagcgattt cgctggcacc gctatagatg gcattggccc
    10621 cgttgcgcac atcaccgacg gtacgcatca gctctccctg catatggcgc aaactctctg
    10681 ccagttgccc catctcatta gagccatcca cctcaatcgg tttcaccaga tcgccgcctg
    10741 caatatgacg aatgctgtca atcaggcgat tcattggcgc taccagcgag gctttaatac
    10801 cgaaccagac ggcgaagatg accgccagta cgacgatcat cacgcccacc agaatccaca
    10861 tcgcctggct gtaggaggca ttgttatcgc tgacggcgat atcatagagc cgatcgtttt
    10921 gctccatgta agccacatac tgcttctcga aaccgtcctg atatccctgg gtcggctgat
    10981 caaagaactc gttgattttg cctgcaccta acagttggat cagctccgcc agcgcattgt
    11041 gataaatatc gtaattacgt ttgatctctg ccgctgcggc ggtgctctga cgcgggtcac
    11101 gcggcaacgc ttcgtaatcc gcccagtttt tttccgcctg tttcagcgaa atactggcac
    11161 tctgcatcag ctcagcaacg gttgaaccgc taccaatatt attctgatcc atcatgtagc
    11221 ggatacccgc gcggttgagg gtgttacgcg tctgcaacaa cgcgacccag ctgccattca
    11281 gcgtgggttg ctgctggcga atggtttgta aaacagtgaa attttctttg tcattcttta
    11341 aggcattaaa aaacagaccg cctgatgtca gttgtaaaag gccaaaaacg gccaaaacca
    11401 gcagtaagct ggtcacgatc ttgatacgtt ttaacatggt ttctctttcc tgtggaccga
    11461 tgcaagattt tcggcctgga aagcaaaaac tttatgaaaa ggtgtaaccc gccatccatg
    11521 gaaaaaccag gcgctggtta tcagatacta aaaagttatt cattatcgcc cgggatgtct
    11581 tcttgtcggg aattgcccag agcaataaat tcctctaaca gatgggttaa ctgttgcatt
    11641 ttctcggcag taaactgagc ttcaatctgc cgataggctt cttcaatctg tgtctgagcg
    11701 cggttataca gcgcctgccc ctctttggtc agcgagatat agagcttccg ctgatcattg
    11761 atcggcttta atcgcaacac taaaccgtcg cgttccatcc gcgtgaggat cccggtcagg
    11821 cttgggcgca aaatgcaggc gcgatacgcc agatcgtgaa aatccattga tgggctttcc
    11881 gccagaatac gcacgatgcg ccactgttgc tcagtcaaat tatgccgttt aacgattggg
    11941 cgaaaataac tcatcgccgc ttctcgcgcc tggagcaacg caatggttag tgagtcgtgc
    12001 attatctttc ccctgaaaaa ttcaaaatca ttaatataga aacaattaat catcacgtta
//
LOCUS       DAZPAB010000112        11912 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZPAB010000112 DAZPAB010000000
VERSION     DAZPAB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11912
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.112"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..2251)
                     /locus_tag="ACHUPG_002719"
     CDS             complement(<1..2251)
                     /locus_tag="ACHUPG_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HFR0862270.1"
                     /translation="MGTGLAKADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTEGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFRDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTLYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVE"
     gene            complement(2275..2898)
                     /locus_tag="ACHUPG_002720"
     CDS             complement(2275..2898)
                     /locus_tag="ACHUPG_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFR0862271.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2895..4583)
                     /locus_tag="ACHUPG_002721"
                     /pseudo
     CDS             complement(2895..4583)
                     /locus_tag="ACHUPG_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="ACHUPG_002722"
     CDS             complement(4732..7359)
                     /gene="gyrA"
                     /locus_tag="ACHUPG_002722"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR0862272.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7506..8228
                     /gene="ubiG"
                     /locus_tag="ACHUPG_002723"
     CDS             7506..8228
                     /gene="ubiG"
                     /locus_tag="ACHUPG_002723"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR0862273.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8356..11912)
                     /gene="yfaL"
                     /locus_tag="ACHUPG_002724"
                     /pseudo
     CDS             complement(8356..11912)
                     /gene="yfaL"
                     /locus_tag="ACHUPG_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2754 a         3302 c         2943 g         2913 t
ORIGIN      
        1 attcgacatt gaccagttca ccaggctggt aatgggtttt gtccgttttc acccggatat
       61 caagctgagg aacggcaact ttgatcccgg cgttctgaaa actgtactga ccattacggg
      121 tatacagcac cgaaaaagtg atgttaggcg caaaggaatt gctcactggt acccgggctt
      181 catacagggt atcgtttaaa cgttgtagcg ttagccagtt tgccggatgc gaaagcagcg
      241 actgctgttc cacgcgatcg cgttccagcg tcaataacgc ttcatcaatt ggctccggga
      301 aagtaatcag catcttcgcg gtttcgcctg gctggtacag cgttttatcc gccacgatat
      361 ctaccgtacc agtatgcgcc gtgctgccct taccgctgac ggcatgactt aacccagcga
      421 gaattaagcc gtctttatcg cgtaatgtca gattgtagtt gccaggttta gcgaaattga
      481 cggtaaagga tttgccgcct gacggtagct ctccgctatg gctcgtgcgg tcttcgagac
      541 gcaaccattc atacgtaaca ggaacctgtt ttgaagattc cagcgcggca taacggaaca
      601 caaccgactc gccgctatta ctgtattgtg cggcggtact taaagagtaa tgcgccaggc
      661 cgcgttcaat gaggatctct ttggtggtgg tgacgcgata cgccgcgccg tcactggcgg
      721 agacggttaa caaatagcgg ctcggtttat cggcggcggg gagatttaac gccacatgac
      781 cgctggcgtc ggacaccgtt tcgctgcctt ccagcgacac ggggaaacgt ccggcataac
      841 gcaaatcgtt accgaccatt gataattgct gagcgcgcaa acttaactgc acgcgggcat
      901 tttttaccgg ctcgccatcc gggtagagca gttgcagttt gccgctgacc gcttcgccag
      961 ttttgaactc ttttttgtcg agagctaaac caatctcgaa atgtggcttg atgtagtttg
     1021 ccacgcgaaa actgctgcta tagacctgat tgcggtaagc aagacgtaac tcataacctc
     1081 cggcaacggc attttctggc aggcggaaac ttccctgccc gccattgcgc gcatccagcg
     1141 tgacatcgac ggtttgcaac agactgccgt tagcgtccag caccgaaagc ttcgccgggg
     1201 cgctgacgat gggggatgaa tgcaacggat cgcggaactc gcggcccatc actttaacat
     1261 cgacacgatc gcctgcgcga tatagcgggc gatcggtaaa aatatacaag cgggtgttgt
     1321 agatttcgct ttcgtagaag aagttctcgg agacaaaaac gccgccttca gcatccttac
     1381 ccagaatgta tgaacgttct ggcgatatat gttgtaactg caaagtaccg ctgtcatcgg
     1441 tcaccccgcg ggtcatcacg ccaagacctt cagtccacaa gatctctgag ccgggcttcg
     1501 cttcaccctg ttttttaccc gcggtccata ccagaagctc gttacctgac actttgctaa
     1561 gcgccacggt atcggaaaca aacaccaccg tcgtcgcccg atacccacct accatcgcct
     1621 cgacgaggta cagccccggc tcttgttggc cgagaggaat ataaatatta cccggctgcg
     1681 gcgagatgaa attgctggat gcgccttcca gttttacccc ttgctgcggc tcgaccggtt
     1741 tagcctgcca tagtggataa cggaactgtt ccaccaaggg atattttttc agcggggaaa
     1801 actggttgtt ctgtacataa cgggaaggtt taataatggc attgccgaga tgtaattcgg
     1861 gtaatgcctg agtcacattc tgccgtgact gagaagagaa agtacgctgc atcacgcggc
     1921 gagatttgcc gtaccagtta tcccacagcc aggtcagcgt attgttcagc ccgtcgccca
     1981 gatattgcgg ttgcaccaca atgcgatgca ggtttttctg ctggcgcaaa aatgccatcg
     2041 ggtcaggaat acgatatagg cgaacgtcca cgccgccgta ctcttccatc tgatagcgcc
     2101 gataatcacg ccccggcgct tccagtcgca ctttcgcctc ttcactgctg ctaaaactgc
     2161 tgtcagcaag caaaaagaat gttcccccgg cgggcggcgc atagttactg gaaggaagcg
     2221 aatcatcagc tttagcaagc cctgttccca caagagacag ggacaagcac gctgctatcg
     2281 cgccagaaaa tttaaacgat aaatgccaat gaaattggga ttggaatcgt tgggtatcca
     2341 tcgggtgtcc ttccatgtca taagttgttg cagactgact gcgcgcattc cgttgtcagt
     2401 tttcgtggcg cttccggtgt ggtagatgac gtaacgcccc atccagacca ttaagtgctg
     2461 ggcatcgccc tgatcgaaaa aaatcatatc gccaggcagc gcctggttta tgtcttggcc
     2521 aataaactgg ctgttgtact gaatcaaatt aatcgcggtc acgtaggggc cgtttttccc
     2581 gctcccctga ttccagtttt gcgccagttg acgctgttca ggtgttagcg tcatctctgg
     2641 cggcaaatac tggctagata aaccgttact ttttagccat ttactgtcgt gaactttcag
     2701 cgtctcgttc gccgcaaatc gcaccaggcc cgcacaatcc tgctgatacc agcgtggact
     2761 tggcccctgg cggagctgtt cttgtgcaat gcgtacaaac caggcgcgaa acagcctgga
     2821 ttgttcgaca ttcagcattt cgctatgggc aacaacacaa cacagccagc aaatcagcgc
     2881 cagcagcccg tgtctcatag cggctgccag gttatgggta gccactgcca ggcgacaccg
     2941 ggttccatct gggccagctt catgacataa cgcggttgtt gagataaagc gtccagcttc
     3001 ggcattaata aagtttgtgc ggcgttataa aaaaccggtt cgagattctt cggcagactg
     3061 gtcaacgttt cgttacgcag cagtttcgcc acgccttgtg gattgatata gagcggaacg
     3121 atgccatcag tgggtattac atccaccatt gccgggcggg ttttattcag tgtttgcagg
     3181 gcgttattaa ccagcgtgtc atcgagggag aaaagcagcg ttttgttttg catcgccagc
     3241 gacacacgga aaaaataatc cgacattaat tgatcgggtt gcgccgcctg cgctttcgga
     3301 tactgaccgt atcgggaact cacttcgcga caccagattt gcgcttcgcc ctgctgagtc
     3361 tggcttaccg gcaaaacacc ttctggcgct ttgctttcgt gcgcaccaat attttgcgta
     3421 aacagtttcc ctggcaattg cgcctgttcg gcagtgccat caaactgacc gacaaacagc
     3481 ggggtttgca attttgagtc ttcataccag cacagccccg cggcaccgtc taacgcccca
     3541 ttcagcttgt cgttttcctg gctgatgtgc gaaagcatct cttcggcaat accgtgtgaa
     3601 tacggcaccg ccacacagaa gctggctccg gcaggcatac tgttccagac cggcgtaaaa
     3661 tcgaaactgg catctacgct ggcggattca tcatttaacg ccacaaaact gtgccagccg
     3721 tcgttaccca tttcgaagcg tacgccagca aaagaaggca ttaaccgctg gtagccaaaa
     3781 cccagcaacc tggcgctgac cacgatgcgc aggcgtactg gcgttttttc ggctgtacgc
     3841 tcttccaggc caaagctggc ttgccagcgt tttttaccgc tcaacaaatc acttgcgatc
     3901 gccgtggctt cggtatcctg ctgatcatct ttaaacaaca tatccgtact ggaaaacacc
     3961 agcatcttgt cctgatatgt cgcgaacatc agggcgttat tgccgttata gcgcaactga
     4021 taaacaggaa tagtttcact atttatcttg atgctactga tttccgtttt gcttaactag
     4081 ctgtcgctgg tagcggcaaa caacaatggc tccagcagtt tgcttaaacc actgcgctgg
     4141 atcagcacca tgtaatgtga aagatggcct tgtttatcgt gccatagcgc cgcctgcgcg
     4201 ggctgatcta agagtgacga aaagagctta tctttcagcg tgagatcgtg ttcatagaca
     4261 atgcggcgaa tgctgccttc aatgcccaga cgatcggcat gattctgata atagaaaacg
     4321 aaatcttcgc tcagaacatc gtggagaaac ggaatggtga ggagatcttt gggaagctgg
     4381 ctcagagagt cgctgtctag aaagaggtcc ggctcattga gatcgatttg cagattgttg
     4441 tgcaccacca gcggcgacaa cgttttttct ggcccactgc cagcatattg caacgcccag
     4501 acgccagcgg aaagcagtgc tattgcgcca aaacctacaa gaccatagaa ccgccagcct
     4561 ttcgcctttt tttcaccact cattgccaca ttccttgtgt atagccagcc attttttacg
     4621 ggcacagcca aactttaccg cgccctaata cgacaaaagc ccagactttg cagcctggac
     4681 ttttcaattc aaacaaggga gatagctccc ttttggcatg aagaagtaaa attattcttc
     4741 ttctggctcg tcgtcaacgt ccacttccgg agcaatttca tcatcccctt ccgcggcact
     4801 gccgtcgatg gtatccagat cttcctcgtc aaccggttca gcaacacgct gcagacccac
     4861 tacgttttca tcttccgcag tacggatgag gatcacgccc tgggtgttac ggcccacgat
     4921 gctgatttct gaaacgcgag tacgtaccag cgtaccggca tcggtgatca tcatgatctg
     4981 gtcgcagtca tctacctgca ccgcgccaac cactaaaccg ttacgttcgg taaccttgat
     5041 ggagataacc cctttcgtcg cacgcgactt ggttgggtat tccgccactg cagtacgttt
     5101 accgtaaccg ttttgcgttg cggtgaggat tgcgccatcg ccacgaggca cgatcagaga
     5161 gacgacttta tcgccttcac ctaagcgaat accgcgcaca ccagtggtat tgcagcccat
     5221 cgcacggaca gaagactctt taaagcgcac cactttacct tcggcggaga acagcattac
     5281 ttcatcttcg ccactggtca gatcaacgcc gatcagctca tcgccgtcaa ccagtttgat
     5341 cgccacttta ccggcggtac gcagacggtt gaattcggtg aggacggttt tcttcacagt
     5401 accgttagcg gtcgccatga agactttcac gccttcttca aactcggtca ccggcaggat
     5461 cgcggtgata cgttcgtcct gttccagcgg tagcaggttg acgatcggac gaccgcgcgc
     5521 gccacgagtg gcttccggca actggtaaac tttcatcgaa tagacgcgac cacggctgga
     5581 gaagcacaga atatggtcgt gcgtgttcgc caccagcagt cggtcgataa agtcttcttc
     5641 tttaatacgt gcggcagatt tacctttccc gccacgacgc tgcgcttcgt attcagaaag
     5701 cggctgatat ttaacgtagc cctggtgaga gagcgtcacg accacatctt cctgggtgat
     5761 cagatcttcc aggttgatgt ctgcgctgtt ggcggtgatt tcagtacgac gtttgtcacc
     5821 gaactgttca cgaaccagct ccagctcttc gcggatcact tccatcagac gatcggcgct
     5881 accaagaata cgcaacagtt ccgcgatctg atccagcagc tctttgtatt cgtcgagcag
     5941 tttttcgtgc tcaaggccgg tcagtttctg caaacgcaga tccagaatcg cctgagcttg
     6001 ctgttcggtc aggtagtaca gaccatcacg cacgccgaac tctggctcca gccattccgg
     6061 acgcgcagca tcgtcgccag cacgttccaa catggcggca acgttgccca gctgccacgg
     6121 attagcaacc agcgcagttt tcgcttctgc aggcgtcggc gcatgacgga tcagttcgat
     6181 gatcgggtca atgttcgcca gcgccacggc taatgcttca aggatatgag cacgatcgcg
     6241 agctttacgc agttcgaaaa tagtacgacg ggtcaccact tcacggcggt gacgaacaaa
     6301 cgccgcgatg atgtctttca ggttcatgat cttcggctga ccatggtgca atgccaccat
     6361 gttgataccg aaagaaacct gcaactgggt ctgggagtag aggttgttga gcacaacttc
     6421 accgaccgca tcgcgtttca cttcaatcac gatgcgcata ccgtctttgt cagactcgtc
     6481 acgcagcgcg ctgatgcctt ccacgcgttt ttcttttacc agttccgcaa tcttctcgat
     6541 caggcgcgct ttgtttacct gatacggaat ttcgtggacg ataatggttt cacgtccggt
     6601 tttggcgtca acttccactt cagcacgggc gcggatatat accttgccgc gaccggtacg
     6661 gtaagcttct tcaataccgc gacgaccgtt aatgattgcc gccgtcggga agtccggccc
     6721 cgggatgtgt tccatcagcc cttcaatgct gatgtcttca tcatcgatat acgccagaca
     6781 accgttgatg acttccgtca ggttgtgcgg cgggatgttg gttgccatac ctacggcgat
     6841 accggaagaa ccgttcacca gcaggttagg aattttggtt ggcatgacgt cgggaatttt
     6901 ttccgtgccg tcatagttat caacgaaatc gaccgtctct ttttcgagat cggccatcag
     6961 ttcatgggca attttcgcca gacggatttc cgtataacgc attgccgccg cagagtcgcc
     7021 gtcgatggaa ccgaagttac cctgaccgtc taccagcatg taacgcagcg agaatggctg
     7081 cgccatacgg acgatcgtgt cataaaccgc cgagtcacca tggggatggt atttaccgat
     7141 tacgtcacca acgacacggg cagatttttt ataggctttg ttccagtcat tgcctagtac
     7201 gttcatggcg taaagtacgc gacggtgtac cggcttcagg ccatctcgga catctggcag
     7261 cgcacggcca acaatgaccg acatcgcata atccagatag gagctcttta gctcttcctc
     7321 aatgttgacc ggtgtaattt ctctcgcaag gtcgctcatc taaccgctat ccctctactg
     7381 tatcccggat tcaaaggtcg caaattataa cacagccgcg cagtttgagg taaacctata
     7441 cgctttattc acatccaatg cctgatatac tcgtttgtct tgccaattac ggagtagaag
     7501 tgccaatgaa tgccgaaaaa tcgccggaaa accataacgt agaccacgaa gagatcgcta
     7561 aatttgaagc cgtcgcctcc cgctggtggg atcttgaagg tgagttcaaa ccgttgcacc
     7621 gcattaaccc gctgcgtctg ggctatattg ccgagcgtgc tggcggttta tttggcaaaa
     7681 aggtgctcga tgtcggttgt ggcggcggca ttctggccga gagtatggcg cgcgaaggcg
     7741 cgacggtgac cggtctggat atgggctttg agccattgca ggtggcaaag ctgcacgcgc
     7801 tggaaagcgg cattcaggtg gattacgtgc aggaaaccgt ggaagagcac gcggcaaaac
     7861 atgccgggca gtatgatgtg gtgacctgca tggagatgct ggagcacgta cccgatccgc
     7921 agtcagtggt cagagcctgt gcgcaactgg tgaaaccagg cggcgatgtc tttttctcga
     7981 cgcttaaccg caacggcaag tcatggctga tggcggtggt tggcgcggaa tatattttgc
     8041 gcatggtgcc caaaggtacg catgatgtga agaagtttat taaaccggca gagctgcttg
     8101 gctgggtgga tcagaccagt ttaaaagagc gacatataac cgggctacat tacaacccga
     8161 tcactaattc ctttaagctc ggccccggcg tggatgtgaa ctatatgctg cacacgcaga
     8221 ataagtgagg ttgatgtttg gccgcgccaa tgcctgatgc gacgcttgca gcgtcttatc
     8281 aggcctacaa atgctctccg taggccagat aaggcgttta cgccgcatcc ggcaattgtg
     8341 atggtcagac agcgattacc atttcaccgt catcgacaaa aaccctgccg tctgggcaaa
     8401 atcatcactc cctttctgcc acgccacgct gccgcgcagg gacactcgct gactgatatt
     8461 gcccgtgact cccactttta tttcaccccg tagcttcacc gcatcgtcac tgatagtgct
     8521 gccatcttct tcaatttccg tcgcatgggg atcgtgatag taattcagat ctaatgttgg
     8581 tgtgacacca accgcggtac gccattcgct gtgtaaaccc agccgcgtct gaatatcatc
     8641 cccctgcgat tgtgacacgc gcgcatggtt agcggcggta aaatcatcct gctgcacgcc
     8701 ctgataaatc acctgcgcct gcggttcaat caccacacca cgccccggta accactgata
     8761 cccagcttcc agcgaggcga taatccccga cgaatggtaa cgatccacgc catcttcatg
     8821 ttcggaaaca tcattgttaa accacgcgta ttgcagccag gccccctgct tctgattacc
     8881 gtgctgatac cagcttgagg tcagcccaac ggcataaccg tggttctggt tatcggcgcg
     8941 agttccggtc atattcgagc ggctgtcgcc ctgattatca ctgtagccac caaccgcccc
     9001 aagcatccac tcgccatccg tgccccagcg cccgctaaac aagccgccgc taagctgcac
     9061 cgtagaagtg tcttcctgct gagccagttg ccctgccgcc gtgtaatgat aacgtccgcc
     9121 gataacacgt aaattcagcg tctgaccatc gccaccagcg tgatcgcgtc gctccatcat
     9181 aaacgcctga tttgccgccc gcaggttatt gagataaccg ccaactttgg cattcagcac
     9241 cggctggtaa gcaggcgtag gggccgggtc gggggttggg tcaggatccg gcgtgggatc
     9301 aggatcggga gtcgggtctg gctcaggtgg cgatggcggc gttacttctt gcgatcgcag
     9361 ataccagtcg ttgttatctt ccactagcgt gtagtcatac gctcccatgt tgacgtagcc
     9421 actgccagcc agactgaact gtgcattgtt ttgaaactgc gtgggatcag ctgcgaaatc
     9481 aaccacttta atgcctgtcg atgtcggctc accaatccct gtaatggagt taaccaccac
     9541 agttgtgttg ccagcagtat taccgttcat caccaattga tcgcttaccg agtcatcgcc
     9601 gtttaattcg ctgtcgaata gtagcgtacc gccgccggta taatcaccgt ttaccgtcag
     9661 cgtatcgcca gcgacgccat tttgcaggct aaccgcaccg gacgtattgg tcagatcacc
     9721 attaaccgtc acgtcagcaa gataggtcag gctgtcagtt tcaaggttgg tattaagctc
     9781 gcctgtcaac gtcacgccat cggtagcatt taccagcgtc ccatcgccgt taaatagtga
     9841 ggcattaagg gcgacggaag tatcctgccc ggtcagacgc aaaaccgtac catcgctgat
     9901 gtcgatagtg ccgctggaag tagcatcaat tgactgaata tcctgatccg cgccagtaac
     9961 gaacgttgcc ccatcaccaa cccacagtga cgaagcataa ggaaagatat ccgcaacatc
    10021 gcctttcagg gtgccttctt cgacacgtac cgccgactgc gttgtaccgc tggcggtcag
    10081 ctccagcgta cccgcccccg ttttagtaaa tgtgctaccc tcatcctgat gctgcccgct
    10141 gctgtcagcc atcagtgcgc cccactgcgt gtctaccccc gcatcaactg ccacttcacc
    10201 gtcggcgcgc atttcaatat cacgcccatg ctcagtcgtc gtagagcgag tatcgacatt
    10261 ggcagtaatc tccatcacgc tttcttgtta cttatcggta aagataacct ggcggttata
    10321 gtgggtatct ccaagctgcg agttgtcgct caccatcagc gtaccggagg cgatttgtgt
    10381 tgtgcccaaa taactattat tgtttgccag acttacctgc ccgtcgctcg catcgaccac
    10441 cacgtagacg ccatctttcc cgtcgccgcc gatatgcaaa ttgtcgccac ctgcaagatc
    10501 taccacatcc tgactgccga taaccgttcc gctgctgcca ctgacttcaa ggccatcatt
    10561 gtatgatgtg ccgctctgcc aagtggagaa atcagagaga tcgagcacac cgccgtttaa
    10621 cacgatcgac tgcggatcgt cctggagagc ggtaagatct gccgcgtcgc cttccagcga
    10681 aagcaccgca ccatcatcaa cgactatatc gccggttagc gccatcgact gcgcccctgc
    10741 cagcacgtag ctgccgtttt gcgcaatggt gagctgacca gcaccttcga tgatgccagc
    10801 aaaactgccc tgattaacag taacgttgcc accagcatcg atatttaaag tgccattctg
    10861 aaagcccgtc aatgcgtgca caaaagtttg ttgggtcgag ccaacgttta gctcagcctg
    10921 attctgatac tgatcaatac tccctatcgt cagaccgtag cagtcttgcg gatcgtcctg
    10981 gcaatgcgta tcgccgacat tcatcaggga gttgctgcga cccagggtaa cttcaccgtt
    11041 ttcaatctgc atctcaccag taaagtcatt gttatctgca ttaagtacca gatcgccgga
    11101 acctgttttg gtgattaacc cggtaccagc aatagagtca acagctccgt cattctctgt
    11161 attgccgata accagcgttt ttccgtcggc aatatcaaag gtaacttcac ttaagccgag
    11221 atacataaag ccacccgccg cagtggaggg accatctcca tagcctgctg cgctattgtt
    11281 ctcatcgact aacacgccgc cgttctggct gtagctgtca tcaacagaaa tatcaataag
    11341 atagggagcc gtcgcgctat tggtatatat cgccccgcca taaccttcgg ctgtgttatt
    11401 tgtaaaggcg gtgttattta ttatcgtata acctgaagga tgcttgctgt cgctattatt
    11461 atcggtaaca tcgattgccc cgccatcgcc atcactgtaa cttgttgatg tatatgcctg
    11521 gttattgtta aaaacatcat cacttaaata gacgtcatta ttaatggtat aaattgcgcc
    11581 acctttgcca tcattagcga tattattgat ggcgttagtg acacgtaaat cgacggcacc
    11641 agtatcatta gtaccagaag aatagattgc gccaccatag ccgcctgcga cgttaccgga
    11701 aaaaataaca tcagtaagat tcagcgttga gttttcttta gcaaatatgg ccccgccgtt
    11761 attatattct cctgtaacgg tgttattggc aaacagagtc attccagttt cattttctgg
    11821 taatagtgaa aattccgctc cttgttgcaa aaatacggcc ccaccgctgg cattattggt
    11881 catatccgaa aaaaccagcc attgcccatc gg
//
LOCUS       DAZPAB010000113        11878 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZPAB010000113 DAZPAB010000000
VERSION     DAZPAB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11878
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.113"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1..2264
                     /locus_tag="ACHUPG_002725"
                     /pseudo
     CDS             1..2264
                     /locus_tag="ACHUPG_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            2265..3914
                     /locus_tag="ACHUPG_002726"
     CDS             2265..3914
                     /locus_tag="ACHUPG_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR0862274.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3919..4695
                     /locus_tag="ACHUPG_002727"
     CDS             3919..4695
                     /locus_tag="ACHUPG_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR0862275.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            4970..7819
                     /gene="rcsC"
                     /locus_tag="ACHUPG_002728"
     CDS             4970..7819
                     /gene="rcsC"
                     /locus_tag="ACHUPG_002728"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR0862276.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(8019..8669)
                     /gene="rcsB"
                     /locus_tag="ACHUPG_002729"
     CDS             complement(8019..8669)
                     /gene="rcsB"
                     /locus_tag="ACHUPG_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR0862277.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(8686..11358)
                     /gene="rcsD"
                     /locus_tag="ACHUPG_002730"
     CDS             complement(8686..11358)
                     /gene="rcsD"
                     /locus_tag="ACHUPG_002730"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR0862278.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         2887 a         2850 c         3190 g         2951 t
ORIGIN      
        1 attaacctcg tcgctgaaag gtaaacctgt ttctgcgcag ctaacggtag gcgtggtcga
       61 tgaaatgatc tacgcgctgc aaccagaaat cgcgccgaat atcggcaaat ttttctatcc
      121 gctggggcgt aacaatgtgc gtaccagctc cagtctgtcg tttatcagct atgaccaggc
      181 gctctccagc gagccggttg cgcctggcgc gactaaccgc agcgagcggc gagtaaaaat
      241 gcttgaacgt ccacggcgtg aagaggtgga taccgcggca tggatgccgt cactcacaac
      301 cgataaacaa ggcaaagcgt atttcacgtt cctgatgcct gattcgttaa cccgctggcg
      361 tatcaccgcg cgtgggatga acggcgacgg gctggtcggg caggggcgtg cttatctgcg
      421 ttcggaaaaa aatctctaca tgaagtggag tatgccaacg gtgtatcgcg tgggcgacaa
      481 accggcggca ggactgttta tcttcagtca gcaggataac gaaccggtgg cgctggtgac
      541 taaatttgca ggcgctgaga tgcgccagac gctgacgctg cacaaagggg cgaattatat
      601 ttcgctgacg cagaatattc agcaatctgg cttgttaagt gcagaactgc aacaaaatgg
      661 gcaagtgcag gacagcatta gcacaaaact gtcttttgtg gacaacagct ggcccgttga
      721 acagcagaaa aatgtcatgc tcggcggtgg cgagaacgcg ctgatgttgc ccgagcaggc
      781 gagtaatatc cggctacaaa gtagtgaaac gccgcaggag attttccgca acaatcttga
      841 tgcgttagtc gatgaaccgt ggggtggggt gatcaacacc ggtagccgtc tgatcccgct
      901 cagtctcgcc tggcgttcgc ttgccgatca tcaaagtgcc gccgctaacg acattcgtca
      961 gatgattcag gataaccgtc tgcggctgat gcaactggcg gggcccggag cgcgctttac
     1021 ctggtggggt gaagatggca atggtgacgc cttccttacg gcatgggcat ggtacgccga
     1081 ctggcaggcc agccaggcgc tcggcgtaac gcaacaaccg gaatactggc agcatatgct
     1141 cgacagttat gccgagcagg cggataatat gccgttattg catcgggcgc tggtgctggc
     1201 gtgggcacag gagatgaatc tgccgtgcaa aacgttgttg aaaggggggg atgaagctat
     1261 cgcccggcgc ggaactaaag atgaagattt ctctgaggaa gacatccgcg atatcaatga
     1321 cagcctgatc ctcgatacac cggaatctcc actggcagat gcggtggcaa acgtcttaac
     1381 catgacgttg ctgaaaaaag cgcagttgaa gtccacggtg atgccacagg ttcagcaata
     1441 tgcgtgggat aaagcggcaa acagcaatca gccgctggcg cacacggttg tgctgctcaa
     1501 tagcggtggt gacgctaccc agacggccgc tattttaatg gtttgaccgc tgagcaatcc
     1561 actattgagc gcgcgctggc catgaactgg ctggcgaaat atatggcgac aatgcctcca
     1621 gttgttttgc ctgcgcctgc gggcgcatgg gctaaacata agttaactgg agggggcgaa
     1681 gactggcgtt gggttggtta gggtgtgccg gacattctct cttttggtga cgaattatcg
     1741 ccgcaaatgt gcaggtccgc tggcgtgaac cggcaaaaac ggctcaacaa agtaacattc
     1801 cggtgaccgt tgaacgccag ttgtatcggc ttatccccgg tgaagaagag atgagcttta
     1861 ctctgcagcc ggtgaccagc aatgagattg acagcgatgc gctgtatctc gatgaaatta
     1921 cgcttaccag cgagcaggat gcagttctgc gctatggtca ggtggaagta ccgctcccgc
     1981 cgggagctga cgttgagcgc acaacatggg gcatttcagt caataaaccc aacgccgcga
     2041 aacagcaggg gcaattgctg gaaaaagcgc gtaatgaaat gggcgaactg gcctatatgg
     2101 tgccggtgaa agaactgacg ggaacggtca ctttccgcca tttgctgcgc ttctcgcaaa
     2161 aagggcaatt cgttctgcct cctgctcgtt atgtgcgttc ctatgcacct gcgcagcaaa
     2221 gtgtcgcggc agggagtgaa tggaccggga tgcaggtgaa ataagtgaac tggcgcagaa
     2281 tcgtctggct attggcgttg gttactctgc caacgcttgc ggaggagacg ccgttacaac
     2341 tggtgctgcg cggtgcgcag cacgatcaac tgtatcagct gtcgtcatca ggcgtgacca
     2401 aagtctcagc attacctgac tcgctcacca cgccgttagg gagtttgtgg aaactgtatg
     2461 tttacgcctg gctggaggat acccatcaac cggagcagcc ctaccagtgt cgcggaaact
     2521 ccccggaaga ggtttattgt tgtcaggctg gggagagcat aacgcgcgat accgcgctgg
     2581 tacgttcctg tgggctctat tttgcgccgc agcggttgca tattggcgca gatgtgtggg
     2641 ggcaatactg gcaacagcgt caggcacctg cgtggttagc gtctcttaca acgctgaagc
     2701 cagaaacctc ggtaacggtt aaatcgctgc ttgattcatt agcgacgtta cctgcgcaaa
     2761 acaaagctca ggaagtctta ctggatgtgg tgctggacga ggcgaaaatc ggtgtcgcat
     2821 ccatgctcgg tagtcgggta cgggtgaaaa cctggagctg gtttgccgat gataaacagg
     2881 aaatccgtca gggtggtttt gctggctggt taaccgatgg tacaccgctg tgggttactg
     2941 gcagtggcac cagcaaaacg gtactaaccc gttatgcgac ggtgcttaat cgggttttgc
     3001 cagtgccgac gcaggttgcc agtgggcaat gtgtagaggt ggagctgttt gcccgctatc
     3061 cgctgaagaa aatcacagcg gaaaagagta cgacggcagt aaaacccagc gtacttaatg
     3121 ggcgctatcg tgtgacgttt actaatggta accacattac gtttgtcagc cacggtgaga
     3181 cgaccttact gagcgaaaag ggcaaactga aattgcagtc gcatctcgat cgtgaagagt
     3241 acgttgccag agttctggat cgcgaggcga aaagtacgcc acctgaggca gcgaaagcca
     3301 tgactgtggc gatacgtact tttttacagc aaaacgccaa tcgggaaggt gattgcctga
     3361 ccatccccga cagcagcgcc acgcagcgtg tttctgcttc gccagcgacc acgggggcgc
     3421 gaacgatgac cgcctggacg caggagctca tctatgcagg cgatccggtt cattatcatg
     3481 gcagtcgcgc taccgaaggg acactttcct ggcggcaagc cacggcccag gcggggcagg
     3541 gggagcgtta cgaccagata ctcgcgtttg cttatcccga caacagcctt agccgctggg
     3601 gagctccacg ctcaacctgc cagttattac ccaaagcgaa agcctggctg gcgaaaaaaa
     3661 tgccgcagtg gcggcgtata ttacaaggcg agacggggta caacgaacca gacgtgtttg
     3721 cggtctgtcg tttagtctca ggtttcccct ataccgatcg gcagcagaaa cggctgttta
     3781 ttcgtaactt ctttacgctt caggatcggc tggatttaac ccatgagtat ctgcacttgg
     3841 cctttgatgg ttatcccacc gggcttgatg agaactatat cgaaacgctg acccgacaat
     3901 tattaatgga ctaatgctat gcgaaagatt tttcttccgt tgttactggt ggcactttcg
     3961 cctgtcgctc acagtgaagg tgtgcaggaa gtcgagattg acgcaccgct ctctggctgg
     4021 catccggcag agggcgaaga tgccagtttt tcacaatcga tcaattaccc ggcgtcgtcc
     4081 gtcaacatgg cagatgatca aaatatctca gcgcagatcc gcggcaaaat aaagaattat
     4141 gctgcggcgg gtaaagttca gcagggccgg ctggtggtca acggtgccag tatgccgcag
     4201 agaattgaat ccgatggttc atttgcacgc ccttatattt tcactgaagg cagcaacagc
     4261 gtgcaggtca tcagcccgga tgggcaaagc cggcaaaaaa tgcagtttta ctcaacgccg
     4321 ggtacaggaa ctattcgtgc acgtttacgg ctggttctct cgtgggatac ggacaatacc
     4381 gatctcgatc tccatgttgt cacgcccgat ggcgaacacg cctggtacgg taacaccgtg
     4441 ctgaaaaaca gtggtgcact ggatatggat gtcacgacgg ggtacggacc cgagattttc
     4501 gccatgccag caccagttca cgggcgctat caggtgtata tcaactacta tggtgggcgc
     4561 agcgaaacgg aattgacgac cgcccaactg acgctgatca ccgatgaagg atcggtcaat
     4621 gagaaacagg aaacatttat tgtaccgatg cgtaatgctg gcgagctgac gctggtgaaa
     4681 agttttgact ggtgatccat cccttcacac aacctggctc tctctttttt cagataagac
     4741 tgctgggcga tctgccgacg ccaggatata accattaaca agcgtcggca gattggtcat
     4801 ttcttatttt tcgtactgag aactgtccat ctactccggc tttcctggtt aaggcaatgg
     4861 tttctaagcg atacgcatta ttgcaatggt gacagaacat tgcggtaaca cgcttttacc
     4921 gctaccttaa ccacactcca tcggtcacct gaggcggagc ttctcccctt tgaaatacct
     4981 tgcttctttt cgtacaaccc tgaaagcctc gcgctacatg ttcagagcat tggcgttagt
     5041 gctctggctg ttgattgctt tttcatccgt tttttacatc gttaatgcgt tacatcagcg
     5101 agaatcggaa attcgtcagg aatttaatct gagttccgat caggctcagc gctttattca
     5161 acgcacctct gatgtgatga aagagcttaa gtacatcgcc gaaaatcgct tatcggcaga
     5221 aaacggtgtg ctttccccgc gtggacgaga aacgcaggcg gatgtgcctg cgtttgaacc
     5281 gctgtttgcc gactccgatt gttccgcaat gagtaacacc tggcgaggtt ctctggagtc
     5341 attggcgtgg tttatgcgct actggcgcga taatttttct gcggcttacg atctcaaccg
     5401 ggtattttta atcggcagtg ataacctctg catggccaat ttcggtctgc gtgatatgcc
     5461 agtggaacgc gataccgcgt tgaaagcgtt gcatgaacgc atcaataaat atcgaaatgc
     5521 accacaagat gatagcggca gtaacctcta ctggatcagc gaaggtccgc gcccttgcgt
     5581 cgggtatttt tacgcgttga cgccagttta tctggcgaac cggttgcagg cgcttttggg
     5641 tgtcgagcag accatccgga tggagaactt tttcttaccg ggtacgttgc cgatgggggt
     5701 taccattctt gatgaaaatg gtcataccct gatttcgctt accggaccag aaagtaaaat
     5761 taagggcgat cctcgctgga tgcaggaacg ctcctggttt ggctatacgg aagggttccg
     5821 ggagctggtg ctgaagaaaa atctgccacc ctcatcgcta agcatcgtgt attcggtgcc
     5881 ggttgataag gtgctggaac gcattcgcat gttgatcctt aacgcaattt tgctgaatgt
     5941 gcttgccgga gctgcattgt ttactctcgc gcggatgtac gagcgacgta ttttcattcc
     6001 ggcggaaagc gacgccctgc gactggaaga acatgagcag ttcaatcgca agattgtcgc
     6061 ctccgcgcca gtgggtatct gcattttgcg taccgctgat ggcgtcaata ttttaagtaa
     6121 cgaactggcg catacctatc tcaatatgct tacgcatgag gaccgccaac gactgacgca
     6181 aattatctgt gggcagcagg tcaattttgt tgatgtcctg accagcaaca ataccaatct
     6241 gcaaatcagc ttcgtccatt cgcgctatcg taatgaaaac gtggccattt gtgtgctggt
     6301 ggatgtttct tcgcgcgtga agatggaaga gtcgttgcag gagatggcac aagcagcgga
     6361 acaggcgagc cagtcaaaat cgatgttcct tgccaccgtc agtcatgagc tgcgaacgcc
     6421 gctgtatggc attatcggta acctggatct gttgcaaacc aaagagttac cgaaaggcgt
     6481 cgatcgtctg gtgacggcaa tgaacaactc ttccagcctg ttgttgaaaa ttatcagcga
     6541 tattctcgat ttctcgaaga ttgaatcgga acagttgaag atcgaaccgc gtgagttttc
     6601 accgcgtgaa gtgatgaacc acatcaccgc caactattta ccgctggtgg tacgcaagca
     6661 gttaggcttg tactgcttta ttgaaccgga tgtgccagtg gccttaaatg gcgacccgat
     6721 gcgtttacag caggtcatct ccaacctgtt gagtaacgcc ataaaattca ccgataccgg
     6781 ctgtatagtt ttgcatgttc gcgctgatgg cgattatctc tctatccgtg ttcgcgatac
     6841 cggcgtgggg attccggcga aagaagtggt gcgcttgttt gatcccttct tccaggttgg
     6901 aacgggcgta cagcgaaact tccaggggac cggtctgggt ctggcgattt gtgaaaaact
     6961 gatcagcatg atggacggcg atatctcggt agattcagaa ccgggaatgg gcagccagtt
     7021 taccgtgcgt attccgttgt acggcgctca gtacccgcag aaaaaaggcg tggaagggtt
     7081 gagtggtaaa cgctgctggc tggcggtccg caatgcgtcg ctctgtcagt tcctggaaac
     7141 cagtttgcag cgcagcggca tcgtcgttac aacatacgaa gggcaggaac cgactcccga
     7201 agatgtgtta atcactgacg aggtagtgag taaaaaatgg cagggcagag cggtagtgac
     7261 cttctgtcgt cgccatattg gtattccgct ggagaaagcg ccaggggagt gggtacacag
     7321 tgtggctgct ccgcatgagc taccggcatt gttggcgcgt atttatttga tcgagatgga
     7381 gagcgacgat cctgctaacg ctctgccgtc gacggacaaa gcggtcagcg ataacgacga
     7441 tatgatgatt ctggtcgtgg atgatcatcc gattaaccga cgtctgctgg cagatcagtt
     7501 gggatcgttg ggctatcaat gtaaaaccgc gaatgatggc gtcgatgcgc ttaatgtact
     7561 tagtaagaat catattgata tcgtgcttag cgacgtcaac atgccaaata tggatggtta
     7621 ccgcttgacg caacgcattc gtcagttggg actgacgttg ccggtaatcg gagtaactgc
     7681 taatgcgttg gctgaagaga agcagcggtg tctggagtcc ggtatggaca gctgcctctc
     7741 gaagccggta acgctggatg tgataaaaca gacgctgacg gtatatgccg agagggtcag
     7801 gaaatcgcgg gattcgtagg acggataagg cgtttacgcc gcatccggca ttcagtgcaa
     7861 atgccagata agacactgac gcgtcttatc tggcctactt taatgtaatc tgttgaaata
     7921 atgggaatcg taggccggat aaggcgttta cgccgcatcc ggcaatcggt gcaaatgcca
     7981 gataagacac taacgcgtct tatctggcct acagatgatt agtctttatc tgccggactt
     8041 aaggtcacag aagagagata attcagcagg gcgatatcgt tctcgacgcc cagcttcatc
     8101 atcgcagatt tcttctggct actgatggtt ttaatactgc ggttcagctt tttagcgatc
     8161 tcggtcacta ggaagccttc cgcaaacagg cgcagaactt cactctcttt tggcgagaga
     8221 cgcttgtcac cgtaaccacc agcactgatt ttttccaaca ggcgagaaac gctttccggg
     8281 gtaaatttct tccctttctg cagcgcggcg agagctttcg gcagatcggt cggtgcacct
     8341 tgtttcagca cgatcccttc gatatccaga tccaataccg cactaagaat cgccgggttg
     8401 ttgttcatag tcagaacaat gatcgacagg cttgggaaat ggcgcttgat gtacttgatt
     8461 aaggtaatgc catcgccgta cttatcgcca ggcatggaga gatcggtaat caacacatgc
     8521 gcatccagtt tcggcaggtt gttgatcagt gctgtagagt cttcaaattc gccgacaaca
     8581 ttcacccact caatttgctc aagtgatttg cgaataccga acaagactat cggatggtca
     8641 tcggcaataa ttacgttcat attgttcatg taataggcta ccttgctaca gcaagctctt
     8701 gacataactg tcaatgtcgc tgatgtattt ttctattcct ggaacatcct tctcacgaat
     8761 cagatgttcc agcgtttcac ataactgctt gccgggtacc agatttagca tggcaaatac
     8821 gcctttaaga cgatgagccg tttgggctaa cgcagcaaag tcactggttg ctgcttcagt
     8881 atacagcctc ttaacatcat ccggtactgt gtctacaaag agcgcataat agccgctggc
     8941 atggagttgc gcattttcat ctccgcccag aggcgattct gtcacctctt cctgtgccag
     9001 ttgcacttca attaattgta agaccgcttc ctgcatagcg ttgctcatat tgaagttgac
     9061 gcacaattga ccaggcccaa tttctcgtac gccagactca tcatcgctta aaagcaagcc
     9121 agaggcagta agattagacg gattatccgt taaaaagata tcataatctt gactaattaa
     9181 tctttcatcg ggtgtgatac aggttgcacc ccaattttct aacaggcgag tgacaatatt
     9241 ccgaatttct gccgaagtaa catccaccat tacgcagaca tcatccagta aacgctcttc
     9301 ttcctcttca acttccgggt cagcggcgag cattttgata tgcacagagt agcgtgtacc
     9361 aagcccatcc cgcgttttga tgtttaaatg accgcccagt ttacgtgcca gttgatcgct
     9421 cagccagaat gccagcgggt ccgcctttcc ataacgatcg ttttgggtct ggttgataaa
     9481 cgggaagtgc aaattatcca tttcatgaat acttacgcct tctcccgtgt ccagaatgcg
     9541 gaatgtcagg cggtcttcag aggactcatc ctgatcaacc tcaagggtga tttttcccaa
     9601 ttgcgttgag gtcacggcat attgcatcag tagcagcaaa atacgtcgta aggcatcgcg
     9661 atcgccgcgg cgcatatcgt gtgctttcag atgattgtta atcagcagtt gcagaccttt
     9721 acgcttgatg gcaggcaaca ctgaaggcac aacttcatca attaaatcct gcacggagaa
     9781 cagcaccgtc tcacttttcc agctatcatc cgcaagcatg ttcgctaact gtatttcttc
     9841 gaccagccgc accagcacat ctgcctgatt cgccagttgt ttgctttccg gggcgttgag
     9901 tttagccgca ctctccgcca gggactgtgc gggttctttc agcgcatcgc caatgttttt
     9961 cataaaggtc atccgcccct gctggttttt ctcatacaga cgctgcgcct gcttgagttt
    10021 cttgtttacc agcacttcgc gatcctgatc gcgaataatg aaaatttgtg tgcgcggcgc
    10081 gacctggctg cggaacatgc ggatctcata cagctcgtta ttgatcgtcg cctgaataat
    10141 cccctgatgc tgttccgcca tggtggtgat gttttgcaga ttcagatgcg gcagcaaatg
    10201 atcggcaatt ttgttactta tgacagtgcg gttcgattcc tgatcgtgaa ccagcaggcc
    10261 gagcggcaac agtgagacta tctcttcatt gattgcccgt aaaatgcgca attcgttatt
    10321 gaccgccgtg ctggggacgc tttctgtact gcggctggag aaatggcgga atgtggtata
    10381 gccaaataac gccagcgcca gcaaaccgat gttcagcagc agtggcagca gaatgttttg
    10441 caacgtatcc agcaataagg tgccataagg aacctgccag accaggcgca tatcggtaga
    10501 gttgagtgcc gaggagattt caatcttcgt actgttaaag tggatactga cgctatccgt
    10561 cccttctttc tcattatcat tgtttcccgt tgccgtcgcg tctggctcaa ggcggaaact
    10621 gtccagcggc atacccggtg ggatcaaatc attaatcggc agatcaaaag ccacgaccgt
    10681 tgccagatgt cccggctggt tgaacgtggt acgcaaggta aagtaatgac cgttctgcca
    10741 ggctaggcgg cgcatgttag aaaaactttc gcgttcatcg agggcgttgg cctgttgtaa
    10801 catttctgca cgacgtgaat caacaatgtc actgacggtc gattctttaa atccggaggt
    10861 gagatcttta aggggtaggg ttgagatcag caccagactg ttatcctgac cattcaggta
    10921 atacatcgac cacggtacat tttctgcacc ccacaatgta tccagataag tggacatccg
    10981 ctgagtcatc tcaagcgttg agttgtcgtg agagccaaag attaacgctt ccgttttgcg
    11041 gcgtggtttc tccagatagt agacatcctg tttcaggcgc gtctcttgta aaccttcgcc
    11101 ggaggagggg gaggtcgtcg cggcaatgtt gtcgtagatc tgcctggtca cgtaacgcca
    11161 ggtatcgacg cgcttttgga ttgcgtgggt aatgtcgaca atctggtaac ttttatcttt
    11221 cagccaagcg ttaacggcac tttgtaccat tacccccatc gtcaccagta acacaatgat
    11281 caacagtaaa aagaagcggg taatgctccc cggtaggagt gaaaagcggg tcgtggccgt
    11341 tgtctctttc tgacgcattc gtgtttatga cctgttaaaa cttcgcgaag tgatagggca
    11401 gtataaaggg tacagtgtga aattccagac tcttacctga ttgatgattc tctattctcc
    11461 cgtgatattt atcatcctgt atggggggaa atagatgtac aagcgccatt ttgctgtaca
    11521 aataacctac aaaaagccca acgaaattaa ttgcaaataa acaaagattg ctggaaatta
    11581 tgctgatgtt aattatttgt gaaatagtta acaagcgtta tagtttttct gtggtagcac
    11641 agaataatga aaagtgtgta aagaagggta aaaaaaccga atgcgaggca tccggttgaa
    11701 ataggggtaa acagacattc agaaatgaat gacggtaata aataaagtta atgatgatag
    11761 cgggagttat tctagttgcg agtgaaggtt ttgttttgac attcagtgct gtcaaatact
    11821 taagaataag ttattgattt taaccttgaa ttattattgc ttgatgttag gtgcttat
//
LOCUS       DAZPAB010000114        11868 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZPAB010000114 DAZPAB010000000
VERSION     DAZPAB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11868
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.114"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            109..1008
                     /gene="lysO"
                     /locus_tag="ACHUPG_002731"
     CDS             109..1008
                     /gene="lysO"
                     /locus_tag="ACHUPG_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR0862279.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1152..2804
                     /gene="hcp"
                     /locus_tag="ACHUPG_002732"
                     /gene_synonym="priS"
     CDS             1152..2804
                     /gene="hcp"
                     /locus_tag="ACHUPG_002732"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR0862280.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            2816..3784
                     /gene="hcr"
                     /locus_tag="ACHUPG_002733"
     CDS             2816..3784
                     /gene="hcr"
                     /locus_tag="ACHUPG_002733"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR0862281.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            3917..5635
                     /gene="poxB"
                     /locus_tag="ACHUPG_002734"
     CDS             3917..5635
                     /gene="poxB"
                     /locus_tag="ACHUPG_002734"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR0862282.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            5672..6673
                     /gene="ltaE"
                     /locus_tag="ACHUPG_002735"
     CDS             5672..6673
                     /gene="ltaE"
                     /locus_tag="ACHUPG_002735"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR0862283.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            6684..8114
                     /locus_tag="ACHUPG_002736"
     CDS             6684..8114
                     /locus_tag="ACHUPG_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR0862284.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8213..9226
                     /locus_tag="ACHUPG_002737"
     CDS             8213..9226
                     /locus_tag="ACHUPG_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR0862285.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9223..10053)
                     /gene="amiD"
                     /locus_tag="ACHUPG_002738"
     CDS             complement(9223..10053)
                     /gene="amiD"
                     /locus_tag="ACHUPG_002738"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR0862286.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10050..10373)
                     /locus_tag="ACHUPG_002739"
     CDS             complement(10050..10373)
                     /locus_tag="ACHUPG_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR0862287.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(10584..>11868)
                     /locus_tag="ACHUPG_002740"
     CDS             complement(10584..>11868)
                     /locus_tag="ACHUPG_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0862288.1"
                     /translation="SKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISE
                     NIDKDAVTNNIALMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFE
                     TKPVHYQDLLTRFSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEV
                     FMSLPVPVATMVREDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVN
                     ESILKKCCFENGSLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVD
                     MIRVNLSKCLFHECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNA
                     GNNVKVAADAFLEIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSC
                     FAAADQKAIQRYRPQSQDGEDFRSHLQYKLPLSAH"
BASE COUNT         2818 a         3067 c         3007 g         2976 t
ORIGIN      
        1 ccctctgatg cataacattt ccttttaatt gcaactttac tcgcccttcc gctttcagta
       61 gtgtgtcatc gcgcatttcg acttcttctt tgcaaggact ggattttcat gttttctggg
      121 ctgttaatca ttctggttcc cctgattgtg ggttacctca ttccgcttcg ccaacaagct
      181 gcgttaaaag ctattaatca gctattaagc tggatggttt accttattct cttttttatg
      241 ggtatcagtc tggcgtttct cgataacctc gccagtaacc tgttggcgat tctgcattat
      301 tctgccgtca gtattaccgt tattttactg tgtaatattg ccgccctgat gtggctggag
      361 cgaggcctgc cgtggcgcaa ccaccatcag caagaaaaac tcccgtcgcg tattgcgatg
      421 gcgctggagt cgctaaaact gtgcggcgta gtagtgattg gttttgccat tggtctgagt
      481 ggactggctt tcttacaaca cgcgaccgaa gccagtgaat acacgttaat tttgctactt
      541 ttcctcgttg gtattcagtt gcgcaataat ggcatgacct taaagcagat tgtccttaat
      601 cgccggggaa tgattgtcgc cgtggtggtg gttgtcagtt cattaattgg tggtttaatt
      661 aacgccttta ttcttgatct ccccatcaat accgcgctgg caatggcctc cggtttcggc
      721 tggtattctc tttccggtat tttattgacc gaatcttttg gtccggtaat cgggagcgcg
      781 gcgtttttta atgatctggc ccgtgaactg attgctatta tgttgatccc tgggctgatt
      841 cgccgcagcc gctctactgc actgggctta tgcggtgcca catcaatgga tttcaccctg
      901 cccgttcttc aacgtactgg cgggctggat atggtcccgg cggcaattgt tcacggtttt
      961 attcttagcc tgttagtgcc gatcctcatc gcctttttct ctgcgtaata cctctctggc
     1021 ggtagttccc tgccgccaaa attgcgctaa atcaatctcc cttaaagttg catgaaaaat
     1081 cccttttatc cccgcgttaa gcgtcttacc cttaaacatg tatattaaat ataactttaa
     1141 aaggtgtgat catgttttgt gtgcaatgtg aacaaactat ccgtactccg gcagaaaacg
     1201 gctgctcata cgcgcagggg atgtgtggta aaacggcgga aacttctgac cttcaggatt
     1261 tactcatcgc ggcgctgcaa gggctttctg cctgggcggt aaaagcgcgt gaatacggca
     1321 tcatcaacca cgatgttgac agcttcgcgc cacgtgcctt tttctcaacc ctgaccaacg
     1381 ttaacttcga ttctccgcgt attgtcggct acgctcgtga agcgattgcc ttgcgcgagg
     1441 cgctgaaagc acaatgcctg gctgtagatg ccaacgcgcg cgtcgataac ccgatggctg
     1501 acctgcaact ggtgagcgat gatctcggcg agctgcaacg tcaggcagca gaatttaccc
     1561 ctaacaaaga taaagcggcg attggcgaaa acattctcgg cctgcgtctg ctgtgcctgt
     1621 atggcctgaa aggtgcggcg gcctatatgg aacacgcgca tgttctcggt caatacgaca
     1681 acgatattta tgcccagtac cataaaatca tggcgtggct ggggacctgg cctgccgata
     1741 tgaacgcgct tcttgagtgt tcaatggaaa tcggccagat gaacttcaaa gtgatgagca
     1801 ttctggatgc aggcgaaacc ggtaaatacg gtcacccgac gccaacccag gtcaacgtca
     1861 aagcgacggc gggtaaatgc attctgattt ccggtcacga tctcaaagat ctctacaacc
     1921 tgctggaaca gaccgaaggc acgggcgtta atgtctacac ccacggcgaa atgctgcctg
     1981 cgcatggcta cccggagctg cgtaaattca agcatctggt cggtaactac ggcagcggct
     2041 ggcagaatca gcaagtggag ttcgctcgtt tccctggccc catcgtgatg acctcgaact
     2101 gcatcatcga cccaaccgta ggcgcttatg acgatcgtat ctggacccgc agcatcgttg
     2161 gctggcctgg cgtgcgtcat ctggatggcg aagatttctc tgcggtcatc gcccaggcgc
     2221 aacaaatggc gggcttcccg tacagcgaaa ttcctcacct gatcaccgtg ggctttggtc
     2281 gccagacgct gcttggtgca gccgatacgc tgattgatct ggttagccgt gaaaaactgc
     2341 gtcatatctt cctgcttggt ggctgtgacg gcgcacgcgg cgagcgccac tacttcaccg
     2401 atttcgccac cagcgtgccg gatgactgcc tgatcctgac cctcgcctgt ggtaaatatc
     2461 gctttaacaa acttgagttt ggcgatatcg aaggtctgcc gcgtctggta gatgcaggtc
     2521 aatgtaacga tgcttactca gcaattattc tggctgtcac tctggcagag aaactgggct
     2581 gtggtgtgaa cgatctgccg ctgtcgctgg tgctctcctg gtttgaacag aaagcgattg
     2641 tcattctgct gacgctgctt tctctgggcg tgaaaaatat cgtcaccggc ccgactgcac
     2701 ctggtttcct gacaccagac ctgctggcgg tgctgaacga gaaattcggc ctgcgttcta
     2761 tcaccactgt tgaagaagac atgaagcaac tgttgaacgc gtaaggagtt tgtcgatgac
     2821 gatgccaacg aatcaatgcc cgtggcggat acaggttcat cacattacgc aagaaacgcc
     2881 ggatgtgtgg acgatttccc tgatttgcca cgatcactac ccatatcgcg ccgggcaata
     2941 tgcactggtc agcgtgcgaa actcagcgga aacgctgcgt gcttacacca tttcctccac
     3001 gccaggcgtg agtgaatata tcaccctgac cgtgcggcgg attgatgacg gtgtcggctc
     3061 ccagtggctg acgcgcgatg taaaacgcgg tgattatctc tggctttcgg acgcgatggg
     3121 ggaatttacc tgcgacgata aagcagaaga taaattcctg ttgctggcgg caggctgcgg
     3181 cgtcacgccg attatgtcga tgcgtcgctg gcttgcgaag aaccgtccac aggccgatgt
     3241 gcgggtgatc tacaacgtgc gtacgccgca ggatgttatt ttcgccgatg agtggcgtaa
     3301 ctatccggta acgctggtgg cagaaaataa cgttaccgaa ggctttatcg ctggtcgcct
     3361 cactcgcgaa ctgctggcag atgtacctga cttagcttca cgtaccgtga tgacctgcgg
     3421 cccggctccg tatatggatt gggtagagca ggaagtgaaa gcgctcggcg tgacgcgttt
     3481 ctttaaagag aaattcttca ccccagtagc agaagcggcg accagcggtc tgaaattcac
     3541 caaactgcaa ccggcacgag aattttacgc cccggttggc accacgctac tggaggcgct
     3601 ggaaagcaat aacgttccgg ttgtcgctgc ctgccgtgcg ggtgtttgcg gctgctgtaa
     3661 gacaaaagtg gtttccggtg aatatacggt gagcagtaca atgacgctga ccgacgccga
     3721 aatcgctgaa ggttacgtac tggcctgctc ctgccatccg cagggggatt tggttctcgc
     3781 ataatcgcct tatgcccgat gatattcctt tcatcgggct atttaaccgt tagtgcctcc
     3841 tttctctccc atcccttccc cctccgtcag atgaactaaa cttgttaccg ttatcacatt
     3901 caggagatgg agaaccatga aacaaacggt tgcagcttat atcgccaaaa cactcgaatc
     3961 ggcaggcgtg aaacgcatct ggggagtcac gggcgactct ctgaacggtc ttagcgacag
     4021 tcttaatcgc atgggcacca tcgagtggat gtccacccgc catgaagaag tggcggcgtt
     4081 tgccgctggc gctgaagcac aactttcagg agaactggcg gtctgtgccg gatcgtgcgg
     4141 cccaggcaac ctgcacttga tcaacggcct attcgattgc caccgcaata acgttccggt
     4201 actggcgatt gccgctcata ttccctccag cgaaattggc agcggctatt tccaggaaac
     4261 ccacccacaa gagctattcc gcgaatgtag tcactattgc gagctggttt ccagcccgga
     4321 gcagatccca caagtgctgg caattgctat gcgcaaagcg gtgcttaacc gtggcgtttc
     4381 cgttgttgta ttaccgggcg acgtggcgtt aaaacctgcg ccagaagggg caaccacgca
     4441 ctggtatcat gcgccacagc cagtcgtgat gccggaagaa gaagagttac gcaaactggc
     4501 gcaactgctg cgttattcca gcaatatcgc cctgatgtgt ggcagcggct gcgcgggggc
     4561 gcataaagag ttagttgagt ttgccgggaa aattaaagca cctattgttc atgccctacg
     4621 cggtaaagag catgtcgaat acgataatcc gtatgatgtt ggaatgaccg ggttaatcgg
     4681 attctcgtca ggtttccata ccatgatgaa cgccgacacg ttagtgctac tcggtactca
     4741 gttcccctac cgtgcgttct acccgaccga tgccaaaatt attcagattg atattaatcc
     4801 agccagcatc ggcgcgcata gcaaggtaga tatggcgctg gtcggcgata tcaaatcaac
     4861 cctgcgcgca ttgctgccac tggtggaaga aaaaaccgat cgcaagtttc tggataaagc
     4921 gctggaagat taccgcgatg cccgcaaagg gctggacgat ttagctaaac cgagcgagaa
     4981 agccattcac ccgcaatatc tggcgcagca aattagtcat tttgccgccg atgacgccat
     5041 ctttacctgt gacgtcggca cgccaacggt atgggcggca cgttatctga aaatgaacgg
     5101 caagcgtcgc ctgttaggtt cgtttaacca cggttcgatg gctaacgcca tgccgcaggc
     5161 gttgggtgcg caggcaaccg agccggaacg tcaggtggtc gccatgtgcg gcgatggcgg
     5221 ttttagcatg ttgatgggcg atttcctctc agtagtgcag atgaaactgc cggtgaaaat
     5281 tatcgtcttt aataacagcg tgctgggctt tgtggcgatg gagatgaagg ccggaggtta
     5341 cctgacagac ggtactgagc tgcacgacac caactttgcc cgcattgccg aagcgtgcgg
     5401 cattacgggt atccgtgtag aaaaagcctc tgaagttgat gaagccctgc aacgcgcctt
     5461 ctctatcgac ggtccggtgt tggtggatgt ggtggtcgcc aaagaagaat tagccattcc
     5521 accgcagatc aaactcgaac aggccaaagg ttttagcctg tatatgttgc gcgcaattat
     5581 cagcggacgc ggcgatgaag tgatcgaact ggcgaaaacg aactggctaa ggtaaaaagg
     5641 gtggcatttc ccgtcataat aaggacatgc catgattgat ttacgcagtg ataccgttac
     5701 ccgaccgagc cgcgccatgc tcgaagcgat gatggccgcc ccggttgggg acgacgttta
     5761 cggagacgac cctaccgtta atgctctgca ggactatgca gcagagcttt ccggtaaaga
     5821 agccgccatt tttctgccta ccggcactca ggccaacctg gtcgctctgc tcagtcactg
     5881 cgaacgtggc gaagagtata ttgtcggtca ggccgcgcat aactatctgt ttgaagccgg
     5941 tggcgcagcg gtgctgggca gtattcagcc gcaacccatc gacgcggctg ccgacggcac
     6001 gctaccgctg gataaagtgg cgatgaaaat caaacccgac gatatccatt tcgcccgcac
     6061 caaattactc agtctggaaa acacccacaa cggcaaagtg ctgccgcgtg aatacctgaa
     6121 agaagcatgg gaatttaccc gcgagcgcaa tctggcgctg catgttgacg gtgcgcgcat
     6181 ctttaatgcc gtggtggctt acggctgcga actgaaagag atcacgcaat attgtgattc
     6241 gttcaccatt tgcctgtcga aaggtcttgg gacgccagtc ggttcattac tcgtcggtaa
     6301 tcgtgattac attaaacgtg ccatccgctg gcggaaaatg gccggtggcg ggatgcgtca
     6361 gtccgggatt ctggctgccg ccgggatgta tgccctggaa aataacgtcg cacggttgca
     6421 ggaagatcac gacaacgccg cctggatggc ggagcaactg cgtgaagcag gcgcggatgt
     6481 gatgcgtcag gacactaata tgctgtttgt tcgcgtcggg gaagaaaatg ctgccgcgtt
     6541 aggcgaatac atgaaagcga gaaacgtgct gattaacgcc tcgccgattg tccgcctggt
     6601 gacgcatctt gacgtctcgc gcgaacaact ggcggaagtc gccgcccact ggcgcgcatt
     6661 cctggcgcgt taaggagaga gacgtgccgc aacgcatttt agttctcggt gccagtggct
     6721 acattggtca gcatctggtg cgcacactca gccagcaagg gcatcagatc ctagcggcgg
     6781 cacgtcatgt cgacaggctt gcaaagctgc aactggcaaa cgtcagttgc cataaagtcg
     6841 atctcagctg gccggataac cttccggccc tgttgcagga tatcgatacc gtctattttc
     6901 tggtgcacag catgggcgaa ggcggcgatt ttatcgctca ggagcgccag ctggctctca
     6961 acgtccgcga tgcgctacgt gaagtaccag ttaagcaatt aatctttctc agttcgttgc
     7021 aggccccgcc acatgagcag tcggatcatc tgcgagctcg tcaggctacg gcggacattc
     7081 ttcgtgaagc gaatgtacca gtgaccgaac ttcgggccgg aattatcgtt ggcgcaggtt
     7141 cagcggcgtt cgaagtcatg cgcgatatgg tctacaacct gccagtgtta acgccgccac
     7201 gctgggtacg ttcacgcacc acgcccatcg cgctggaaaa cttgctgcac tatctggtgg
     7261 cgctgttaga ccatccagcc aacgaacacc gcatcttcga agccgccgga ccagaggtgc
     7321 tcagttatca gcaacagttt gaacatttta tggcggtgag cggtaagcgc cgctggttga
     7381 tccccatccc cctccccacc cgctggattt cggtgtggtt tctcaatgtg attacttccg
     7441 taccgcccac caccgccagg gcgttgattc aggggctgaa acacgatctg ctggcagatg
     7501 ataccgcgct acgtgcactc atcccacaac ggctgatcgc tttcgatgac gcggtacgta
     7561 gcacgttgaa agaggaagaa aaactggtca actccagcga ctggggctac gacgctcagg
     7621 cctttgcccg ctggcgaccg gagtacggtt atttcgccaa acaggcgggg tttaccgtta
     7681 aaacgtccgc cagccttgcg gctttatggc tggtggtgaa ccaaatcggc ggtaaagagc
     7741 gttatttctt tggcaatatt ttgtggcaga cacgggcgtt gatggaccgc gcgatcggtc
     7801 ataatctggc gaaaggccgc ccggagcgcg aatatttaca gaccggcgat gcggtggata
     7861 gctggaaagt gattgtcgtt gaaccgaaaa agcaacttac gttgttattt ggcatgaaag
     7921 cgccggggct gggacgactg tgttttaccc tggaagataa aggtgactat cgtactatcg
     7981 atgtccgcgc tttctggcat ccgcacggta tgccggggct gttttactgg ttattgatga
     8041 tccccgcgca tctgtttatt tttcgcggaa tggcaaaaca aatcgccaga ctggcagaac
     8101 aaagcacaga ttaatcgaca aaacctgcgt tattctttca tgtttcccat tgcatcatgg
     8161 gggaaatcac ggaagaatgc gcacgtcatt ttctcaacaa cagtggattg atatgaaggt
     8221 actggttacc ggcgccacca gcggcttagg tcgaaacgcg gtagagtttt tatgccagaa
     8281 aggcatcagc gtgcgtgcga gcggtcgcaa cgaagcaatg ggtaaattgc tggaaaaaat
     8341 gggcgcagag tttgttccga cggatctgac cgagctggtt tcatcacaag ctaaagtgat
     8401 gctcgcgggc attgatacgc tgtggcactg ctccagcttt acctcaccct gggggacaca
     8461 acaggctttc gatctggcta acgttcgcgc cactcgccgc ctgggtgaat gggctgtcgc
     8521 ctggggtgtg cgtaacttta ttcatatctc ttccccctcc ctgtacttcg attatcacca
     8581 ccatcgcaat attaaagaag attttcgccc tcaccgcttc gccaacgagt ttgcccgcag
     8641 caaagcagcc agcgaagaag tgatcaatat gctttcgcag gcgaatccac aaacgcgctt
     8701 tactattctg cgcccacaaa gtctgttcgg accgcacgat aaagtcttta ttccccgtct
     8761 ggcgcatatg atgcaccact acggcagtat tctgttaccg catggcggca gtgcgctggt
     8821 cgatatgacc tactatgaaa atgccgtgca cgcaatgtgg ctggcaagcc aggaagcctg
     8881 cgataagcta ccttccgggc gtgtgtacaa catcaccaac ggcgagcatc gcacactgcg
     8941 cagcatcgtg cagaagctga tcgacgagtt gaatattgac tgtcgtattc gttccgtccc
     9001 ttacccgatg ctggatatga tcgcccgcag catggagcgt ttaggccgca agtcagcaaa
     9061 agagccgccg ctgacccact acggcgcctc caagttgaat tttgacttta cgctggatat
     9121 tacgcgtgcg caggaagagt taggttatca gccggtcatc accctggatg aaggcatcga
     9181 gaaaactgcc gcctggctac gcgaccacgg aaaactgccg cgctaatcct gcccgtattt
     9241 ctccagcaat gcttcggcaa tcgcctgagt ttctgcatcc gcttcgccgt tatataacgt
     9301 cgggcggaaa tgcatctgga atgccataat cacgcgccgc tgctcgcgcg gtgtcatatc
     9361 aggttttacg tcataaccgt agcgcgccaa aagctccagc aatgacgcgg tatctaccgg
     9421 tgtgtgcggc gcgcgcccgg caaggtaaaa gttcacccgc tgcgcatccg gccaggcacc
     9481 aatcccctgc tgcgccagtt gctgccaggg aaataatggc cccggatcgt ctttgcgctg
     9541 cggtgcgata tccgcatgtg ccactacgtt ttccggcttg atgtgataac gggcaataat
     9601 atctttcgcc agcggaataa gcgcctgaat ctgtgcctgt tcaaacgggg caaaatattt
     9661 cactccggat gatttttgcc agccacggtt ttccagctca atgccaatag aggtgtcgtt
     9721 aaggcgcgtt gccccgcgcc aggcgctaat ccccgcatgc caggccagtt cttgttccgg
     9781 caccagttgc cagatgcgcg gtttaccgtt gtatcgcggt ggcaccgcag ggatcagata
     9841 atgcgagctg acctgcttat cagtcagtgt cgccagcgag ctatcaaaat catctgcggt
     9901 gtagtggatc accagcactt taatgcgcgg atacgccgcc tgcgcctggt gtcgggtatc
     9961 aagctgatat ccctctttct cgacaatgcc tttttcgcct gcacaccctg ccagtaacag
    10021 agcggcagcg accagccaga agactcttct catcgacgcg ttttcaccgc cgtaccgctg
    10081 acgctaacca tcagcatact gccgttttgc ccgaccgttt cgtagtcgat atcaatacca
    10141 actacggcat cggcccccag cgcccgcgcc tgggagccta attcctcaaa ggcgatctcc
    10201 cgtgctttac gcagttcttt ttcatacgca ccggaacgtc cgccaacgat atcgcggata
    10261 ccggcaaaga aatcacggaa aatattggca cctaaaatcg cttcgccggt caccacaccg
    10321 caatattcaa cgatggtcag gccttccaga gttggggttg tagaaaattg catgatcgat
    10381 ctccttgttt gttaagcctt cgatctctaa agcattatcg gactgatatg ccatgttggg
    10441 aagaaggttt tgatcagttt aggaaatcat ctcattgtgt caaaaacacc cacttaagtc
    10501 tttacgatta tccgttgcat aacccgatat tggtttcatt atagggtacc gcttacctgt
    10561 cttattatgt tgcaccaaca acattaatga gcggaaagag gcaacttata ttgtaaatga
    10621 ctcctaaagt cttccccatc ttgtgactga ggtctatacc gctggattgc tttttggtca
    10681 gccgccgcaa aacaagaaag taataagatc tcaatgcttt tgtcattaat ataagcgaag
    10741 atcattcttg caaactcgta tataaacgcc cgatcctgag cgcttaatag tttaatacaa
    10801 tatttatttt taagagagaa aataatttca aggaaagcat ccgcagcaac cttcacgtta
    10861 ttcccggcgt tcatattata tatatccctt gcacagatag agtacagaga tagtaaacag
    10921 ccatacaaat aatcacttat taattctttt gtccatttta caggcaacca gggttttatc
    10981 ttgacaccat gcatactaca ttcatgaaaa agacatttgc tgagattaac tcttatcata
    11041 tcaacattga aaaaattgca gttaataaat tttaaagaat gagtatttac gttattaatt
    11101 ttaacagatc taaacgaaat atttttgaat gcactgttta ttacattgct atttgttatt
    11161 ttaacatcac ctaaactgcc gttttcaaaa caacattttt tcaatatgct ttcattcaca
    11221 acagtgtgtg ataaatagag attacttatg ttttgcttta ttaatgccac gtcattaaaa
    11281 tgactatatt taatgaaaca accatcattg agtagtgttt taagattgac taaattactt
    11341 gcatcttcac gtaccatcgt ggcaactgga acaggcagag acatgaaaac ttcttcattc
    11401 accatcaagt gaatcatgca ttgcgttccc tttgtcatat ccacacttac gctacaaata
    11461 tcgtggtaat gcgaagagtg catttcagga aacagattaa acacggtatc ttgaaaggca
    11521 ctaaatcgtg ttaataaatc ctgataatga acaggttttg tttcaaaatt atcatcagca
    11581 aatccaagca ctattgcatg ctctacatct aacgcagttt caaatgactc ttcttctgta
    11641 aaatcattat ttaaaacctc acctgacgta ttatcactgt cataaaaagt gctcatcaat
    11701 gcgatattgt ttgttacagc gtctttatcg atattctcac taatagcata tcgcctgcta
    11761 taatcataaa aatgaataaa tgcgtccttt ataaaggata tatattgttc tttgttaaag
    11821 tcaatacgac tgtataactt ttcaaaaatc acattcgcaa tcttacta
//
LOCUS       DAZPAB010000115        11793 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZPAB010000115 DAZPAB010000000
VERSION     DAZPAB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.115"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            38..778
                     /locus_tag="ACHUPG_002741"
     CDS             38..778
                     /locus_tag="ACHUPG_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR0862289.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            837..1223
                     /locus_tag="ACHUPG_002742"
     CDS             837..1223
                     /locus_tag="ACHUPG_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR0862290.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1232..1561
                     /locus_tag="ACHUPG_002743"
     CDS             1232..1561
                     /locus_tag="ACHUPG_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR0862291.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1533..4574
                     /locus_tag="ACHUPG_002744"
     CDS             1533..4574
                     /locus_tag="ACHUPG_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR0862292.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4574..4903
                     /locus_tag="ACHUPG_002745"
     CDS             4574..4903
                     /locus_tag="ACHUPG_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0862293.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            4903..5601
                     /locus_tag="ACHUPG_002746"
     CDS             4903..5601
                     /locus_tag="ACHUPG_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR0862294.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5606..6349
                     /locus_tag="ACHUPG_002747"
     CDS             5606..6349
                     /locus_tag="ACHUPG_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR0862295.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6313..6888
                     /locus_tag="ACHUPG_002748"
     CDS             6313..6888
                     /locus_tag="ACHUPG_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR0862296.1"
                     /translation="MQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSDGWYQVRIAG
                     RDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVAIWMPGVGIM
                     ASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMVAQGNVLPVL
                     YGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            6949..10428
                     /locus_tag="ACHUPG_002749"
     CDS             6949..10428
                     /locus_tag="ACHUPG_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0862297.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10496..11095
                     /locus_tag="ACHUPG_002750"
     CDS             10496..11095
                     /locus_tag="ACHUPG_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR0862298.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11159..>11793
                     /locus_tag="ACHUPG_002751"
     CDS             11159..>11793
                     /locus_tag="ACHUPG_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR0862299.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLS"
BASE COUNT         2774 a         2795 c         3790 g         2434 t
ORIGIN      
        1 acgtaccaga tcacgtacac gaattaagga ggtggtaatg accacaccaa atccactggc
       61 aaagacgaaa ggtgcgggga cgacgttctg gatgtatacc ggcaacggcg atgcgtttgc
      121 gaaccctttg tcggacactg actggctgcg tcttgcgatg gtgaaggatc tgcaacctgg
      181 cgaaatgacc gctgatgcag aagatgacac ttatctcgat gatgaagatg cagactggaa
      241 aacgacaacc caggggcaga aatccgttgg tgatacttcg gcgacgctgg cctggcgtcc
      301 gggtgacagc gggcagaaaa aactggttca gttgttcgac tccggtgaag tctgcgcgtt
      361 tcgtatcaaa tatcccaacg gcactgttga tgttttccgc ggctggctga gctcactggg
      421 taaaaccatt gcctcaaaag acgtgatgac ccgcactgtg aaaatcagcg gtgtggggcg
      481 tccgtatctg gcagaggaag gcactgaaac cgtgggtgtt accggactga cggtggcacc
      541 ggcatctgtc agtgtcaaag cgggagcaac caccacactg acctttacag taaaacctga
      601 cggggccagt gacaaagcga tcagtgtgca ttcgtcagat cctcagactg cttcggtgac
      661 cctgagcggg cttgtggcca cggtgaaagg cgtgaagcag ggcagtgtca gcatcgtggg
      721 catgacctct gacggagagt ttgtggcagt ggctgcggtg accgtcagcg caccataaca
      781 ggacgatact catcattgcc ccggttatcc ggggcttttt gcatccggag aacatgatgt
      841 ttctgaaaca ggatacgttt aattatgaaa aacagtccgt ggtgctcagt gagctgtccg
      901 ggctgcagag aattgaatat ctgacgtttg ttcagcagcg aacggcaaag tttgatgcac
      961 aggagggaga actgccggag gctgaacgac agattgcttt tctgcgtatg ggaatggata
     1021 tcaatgcctg gctggtttcc cgctcactgt ggaatgctga gcagtctcag gatgttgaga
     1081 cgctttgcgc atccattatg acaacatggt cgtatgatgc gctgggcgcg ggggcggaga
     1141 gggttctgtc gctgagcggt atggggacca ttgagaatgc cggggatgat gatcatgagg
     1201 cgctgacgcc ggaaaagtcc tgacgcggga aatgcagttt gtcatgcggc ttgcccggga
     1261 gttccggcgg gcagactggc ggcggatgct gtcggatatg tcggccactg agcttggtga
     1321 gtggggcgat tatttccgga tgcagagctt cagtgatgtg tggatggatg cgcagtttgc
     1381 ctcgctgaag gcattgatcg tgagaatggt gtccggcagc agcgatgctg cggtggctga
     1441 tttcagcctt ttaccggaag agaacgggat accggagcga acggacgaag aactgatgca
     1501 tcttggggaa ggtatttccg gaggtgtgcg ttatggacca gatagccaac ctggtcattg
     1561 atttggggat tgatgcggca gagtttaaaa atgaaattcc ccgtatcaaa aaccttctga
     1621 atggtgcagc cagcgatgca gaacggtctt ctgcccgtat gcagcgtttt atggagcgtc
     1681 agactcaggc ggcccggcag acaatgcagg cggcttcttc ggctgcaaca gcagcatcag
     1741 cccatgcgca gacggtggag aagaatgcac gggctcatga acgcatggcc cgtgaggtgg
     1801 aacaaacccg tctgcgcgtg gatgccctga atcagaaaat gcgcgaggaa caggcgcagg
     1861 ccagggcact ggcggaggcg caggataaag cggctgccgc cttttatcgc cagattgaca
     1921 gtgtgaaaca ggccggtgcg gggcttcagg aattacagcg tattcagcag cagatccgac
     1981 aggccagaaa cagtggcggg gttggtcagc aggattatct ggcgctgatt tcggagatca
     2041 cggcgaaaac ccgtgccctg acgcaggcag aggaacaggc cacccggcag aaagcggcgt
     2101 ttatccgcca gcttaaagag caggcaaccc gccagaatct gtcgtcttct gagttgcttc
     2161 gcgccagggc ggctcagctg ggggtaagca gtgctgcaga agtgtatatc cgcaaaatgg
     2221 agcgggcagg aaaagccaca cattcgctgg ggctgaaaag tgcggcagcc cggcgggagc
     2281 tgggggtgtt aatcagtcag atggcgcgag gcaattttgg tgcgctgagg ggatccggga
     2341 taacgctggc taaccgtgcc ggatggacag gcgcactgat gtcgccgaaa ggcatgatga
     2401 ctggcggcgt tattggcgga cttgtcgcgg cggtcctggg tctgggtaaa gcctggcatg
     2461 acggccggaa ggagggcgag aaatttaacc gtcagctggc gctgacggga cattatgccg
     2521 gtgtcactgt cgggcagttg tggaaactca gccgggccat atccgggaat ggtatcacgc
     2581 aacatgcggc agccggtgcg ctggcacagg tggtgggcag tggggcattt catggaaacg
     2641 atattggtat ggtggcgaaa gctgccgcac agatggagcg atcggttggc cagtcggtca
     2701 gcgataccat aaatcagttt aagcggctga aggatgatcc tgtaaatgcc gcgaaggcgc
     2761 tggacaatgc gctgcatttt ttgaatgcca ctcagcttga gcagatacgc gtccttgggg
     2821 agcaggggcg gtccagtgat gcggcccgga tcgccatgtc tgcgctggca gaggaaaccg
     2881 gtaaacgcac atctgatatt gataataatc tcaatgcgct gggtagtacg ctgcaaacct
     2941 tgtctgactg gtggaagcag ttctgggatg cggccatgaa tattggtcgt gaagactcgc
     3001 tggatgcgca gattgatgcg ttacaggaaa aaattcagcg cgcgaaaaaa tatccgtgga
     3061 caaacgcctc cacacaggtg gagtacgatc agcagcgtct taacgatctt caggagaaaa
     3121 aacgccggaa ggatttgcag gatgcaaaag cgcaggcaga acggaattac caggagcaac
     3181 agaaacgccg gaatgctgaa aatgccgcgc tgaaccggat gaatgaaacg gaagctgcac
     3241 gacatcagcg ggaaattgcg cgtattaatg ccatgcagta cgccgaccag gctgtcaggg
     3301 atgcggcgat acagcgtgaa aatgaacgtt acgaaaaagc cattaagaaa aatacacggg
     3361 caacccgtaa tgatgaggcc acccggttat tgctgcagta cagtcagcag caggcacagg
     3421 tggaagggca gattgctgct gccagacagt cagcaggcat tgccactgaa aggatgactg
     3481 aagcgcataa acagcttctg gccctgcagc agcgcatcag cgacctggac gggaaaaagc
     3541 tgacggcaga tgaaaagagt gtgctggccc gtaagaatga gctgattcag gcgctgacgc
     3601 tgctggatgt gaaacagcag gagctgcaga aacagacagc gcttaacgac ctgagaaaaa
     3661 aaacggttca gctgaccagc cagctggcag acaaagaacg tgcactgcgt gagcagcaca
     3721 atctggatat tgccactgca ggtatggggg ataagcagcg gcagcgctac caggcacagt
     3781 tgcgcatccg gcaggaatac cggcaacagt tgcaacagct tgagaatgac agtcgccaga
     3841 aaggcactta cgggacggag gactaccgga gggctgagga ggtgctgaag gggagcctga
     3901 agcgacaact gaatgaaaac aaacgctact ggcaggaact ggaagtggcg cagggcgact
     3961 ggaaaaacgg tgccatgcgg gcgtttcaga attttacggc ggatgcggat aatgcggcgg
     4021 gaacggcaga acagatgttt acagtggcat tcagcagtgc cggtaatgca ctggcgacat
     4081 tctgtaccac cggtaagctg aattttaaat ccttcacctc ttccctgttg tcagatatgg
     4141 ccagaattat ggcacagatg gccatgatgc aggcggtaaa gggcgtcggt tctttattcg
     4201 gcttcacgac taatgctgat ggcggtgttt accagtctgc tgatttgagt cgctacagtg
     4261 gcacggtggt taaccgtccg acgttttttg cttttgcaaa aggcgcgggt gtgatggggg
     4321 aagcgggacc tgaagccatt ctgccactgc gtcgcggtgc tgatggtaag ctgggggttg
     4381 tggcggatac tggtggttca ggcatggtga tgtttgcccc gcagtacaac attgagatca
     4441 ataacgacgg cacgaacggg cagataggtc cggctgccct gaaggtggtt tatgacctcg
     4501 ggaaaaaagc ggccgcggac tttatgcaac agcaggcccg tgatggtggc cggttaagtg
     4561 gagcataccg gtaatggaga cgtttcactg gaaagtgcgc ccggatatga atgtggtatc
     4621 agagccgaaa gtggtgacag tgaagctggg cgatggttat gaacagcgtc gtgcggcggg
     4681 actgaataac cagttgtcga cttacagcgt gacgatacgt gttcgtaaag gtgaacaccc
     4741 atctttaaaa gcctttctgg aacggcacgg tggcgtccgc gcatttcagt ggacgccacc
     4801 ttatgactgg aaactgatca gggtggtttg tcgtaaatgg tcggcaagcg tgggggcgtt
     4861 atgggtgact gtaacggccg attttgaaca ggtggtgaac tgatgcagga tatccggcag
     4921 gaaacactga atgaatgcac ccgtgcggag cagtcagccc gggtggagct ctgggaaatc
     4981 gatctgacag aggtcggtgg tgagcgttat tttttctgta atgagcagaa cgaaaaaggt
     5041 gagccggtca cctggcaggg gcggcagtat caggcatacc ccattcaggg gtcggggttt
     5101 gaactgaacg gcaggggctg tgctgcccgt ccgacactga cggtctctaa cctgcacggc
     5161 atggtcaccg ggatggcgga agatctgcag agtctggtcg gcggaacggt ggtccggcgt
     5221 aaggtttacg cccgttttct ggatgcggtg aacttcgtca acggaaacag cgacgccgat
     5281 ccggaacagg aggtgatcag ccgctggcgc atcgagcagt gcagcgaact gagcgcggtc
     5341 agtgcctctt ttgtgctggc cacaccaacg gagacggatg gcgcggtttt cccggggcgt
     5401 atcatgctgg cgaatacctg tatgtggact taccgttctg atgagtgtgg ttacacgggc
     5461 agggcagtgg ctgacgagtt cgacaaacca acaacggata tccggaagga caaatgcagc
     5521 aagtgtatgc gcgggtgtga gttgcgcaac aataccggta atttcggcgg tttcctttcc
     5581 atcaataaac tttctcagta aatccatgac acagacagaa tcagcgattc tggcgcacgc
     5641 ccggcgatgt gcgccagcgg agtcgtgtgg cttcgtggtg agaacgccgg agggagacag
     5701 gtatcttccc agcgagaata tctccggtga gccggaggaa cggttccgga tggctccgga
     5761 ggactggctg cgggcacaaa tgcagggtga gattgtggca ctggtccaca gtcatcccgg
     5821 tggtctgccc tggctgagtg aggctgaccg gcggctgcag gtgcagagtg atttgccgtg
     5881 gtggctggtc tgccggggga cgattcacaa gttccgctgt gtgccgcatc tcaccgggcg
     5941 gcgctttgag cacggggtga cggactgtta cacgctgttc cgggatgctt atcatctggc
     6001 ggggattgag atgccggatt ttcatcgcgg ggatgactgg tggcgtcacg gtcagaatct
     6061 ctatctggat aatctggagg ccacagggct gtatcaggtg ccgttgtcat cagcacaacc
     6121 gggcgatgtg ctgctgtgct gttttggttc atcggtgccg aatcatgccg ccatttactg
     6181 cggcgatggt gagctgctgc accatattcc tgaacaactg agtaaacgag agaggtatac
     6241 cgacaaatgg cagcgacgca cacactccct ctggcgtcac cgggaatggc acgcatctgc
     6301 ctttacgggg atttgcaacg atttggccgc cgcatcgacc ttcgtgtgaa aacgggggct
     6361 gaagccatcc gcgcactggc cacacagctc ccggcgtttc gtcagaaact gagtgacggc
     6421 tggtatcagg tgcgcattgc cgggcgtgat gcaggtgaaa ccgaattgtc tgcccgtctt
     6481 aatgagccgc tggcaaatgg tgcagtgatc cacatagtac cgcgtctggt gggagctaaa
     6541 agtggcggtg tgtttcaggc ggtgctgggg gcagctgtta tggcggttgc tatatggatg
     6601 ccgggggtag gaattatggc gagtaatctg ctgttttctc tcggtgccag tatgacgctt
     6661 ggcggtgttg cacagatgct ggcaccgaaa gccagaactc cccgtacaca gacaacggat
     6721 aacggcaaac agaacacctg tttctcctca ctggataaca tggttgccca gggcaatgtt
     6781 ctgcctgttc tgtacggtga aatgcgcgtg gggtcgcggg tggtatctca ggagatcagc
     6841 acggcagacg aaggggatgg tggtcaggtt gtggtgattg gtcgctgatg caaaatgttt
     6901 catgtgaaac cgcctgcggg cggttttgtc gtttatggag cgtgaggaat gggtaaaggc
     6961 agcagtaagg ggcatacccc gcgcgaagcg aaggacaacc tgaaatccac gcagttgctg
     7021 agtgtgattg atgccatcag cgaagggccg gttgacggtc cggtggatgg attaaaaagc
     7081 gtgctgctga acggtacgcc ggtcctggat agcgagggga agacaaactt ttccggtgtt
     7141 acggtggtgt tccgcgccgg cgagcaggag cagacaccgc cggaggggtt tgaatcttcc
     7201 ggctcagaga ctgtgctggg tacggaagtg aaatacgaca cgccgatcac ccggaccatc
     7261 acgtcggcaa acattgatcg tctgcgcctg accttcggtg tgcaggcact ggtggaaacc
     7321 acctcaaagg gggaccggaa tccgtcggaa gtccgcctgc tggttcagat acagcgtaac
     7381 ggtggctggg tgacggaaaa agacatcacc attaagggca aaaccacttc gcagtatctg
     7441 gcctcggtgg tggtgggtaa cctgccgccg cgcccgttta atatccggat gcgcaggatg
     7501 acaccggaca gcaccacaga ccagctgcag aacaaaacgc tctggtcgtc gtacaccgaa
     7561 atcatcgatg tgaaacagtg ctacccgaac acggcgctgg tcggcgtgca ggtggattca
     7621 gagcagttcg gtaaccagca ggtgagtcgc aattatcatc ttcgcgggcg cattctgcag
     7681 gtgccgtcga actataaccc gcagacgcgg caatacagcg gtatctggga cggaacgctt
     7741 aagccagcat acagcaacaa catggcctgg tgtctgtggg acatgctcac tcatccgcgc
     7801 tacggcatgg ggaaacgtct tggtgcggca gatgtggaca aatgggcgct gtatgtcatc
     7861 ggccagtact gcgaccagtc agtgccggac ggatttggcg gcacggagcc gcgcatcacc
     7921 tgtaacgctt acctgaccac acagcgtaag gcgtgggatg ttctcagtga tttctgctcg
     7981 gcgatgcgct gtatgccggt atggaacggg cagacgctga cgttcgtgca ggaccgaccg
     8041 tcggataaag tgtggaccta taaccgcagt aatgtggtga tgccggatga tggcgcgccg
     8101 ttccgctaca gcttcagcgc cctgaaggac cgccataatg tcgttgaggt gaactggatt
     8161 gatccggaca acggtcatga gacggcgaca gagcttgtgg aggacacgca ggccattgtc
     8221 cgttacggtc gtaacgtcac gaagatggat gcctttggct gtaccagccg gggacaggcg
     8281 caccgcgccg ggctgtggct gattaaaacg gaactgctgg aaacgcagac cgtggacttc
     8341 agcgtcggcg cagaagggct tcgccatgta ccgggtgatg tcattgaaat ctgcgatgat
     8401 gattatgccg gtatcagcac cggtggtcgc gtactggcgg tgaacagcca gacccggacg
     8461 ctgacgctcg accgtgaaat cacgctgccg tcctccggta ccacgctgat aagcctggtt
     8521 gacggaaatg gcaatccggt cagcgtggag gttcagtccg tcaccgacgg cgtgaaggtg
     8581 aaagtgagcc gggttcctga cggcgttgca gaatacagcg tgtgggggct gaagctgccg
     8641 acgctgcgcc agcgcctgtt ccgctgcgtg agtatccgtg agaacgacga cggcacgtat
     8701 gccatcaccg ccgtgcagca tgtgccggaa aaagaggcca tcgtggataa cggggcacac
     8761 tttgacggtg accggcgcgg cacggtgaat ggtgtcacgc cgccagcggt gcagcatctg
     8821 accgccgaag tcaccgcaga cagcggggaa tatcaggtgc tggcgcgatg ggacacgccg
     8881 aaggtggtga agggtgtgag cttcctgctt cgcctgaccg tggcagcgga cgacggcagt
     8941 gagcggctgg tcagcacagc caggacgacg gaaaccacat accgcttcag gcagctggcg
     9001 ctggggaatt acagtctgac agtccgggcg gtaaatgccc gggggcagca gggcgatccg
     9061 gcgtcggtat cgttccggat tgcggcaccg gcagcgcctg tcactattga actgataccg
     9121 gggtattttc agataacggt ggtcccgaaa ctggctgtat atgacccgac ggtgcagttt
     9181 gagttctggt tttcggaaaa gcggattgcg gatatcaggc aggttgaaac cagcgcccgc
     9241 tatcttggca cggcgctgta ctggatagcc gccagtatca atatcaggcc gggccatgat
     9301 tattattttt acgttcgcag tgtgaacacc gttggcaaat cggcatttgt ggaggccgtc
     9361 ggtcgggcga gcgatgatgc ggaaggttat ctgagttttt ataaagggtt gatcaataaa
     9421 acgcatctcg gcaaggagtt gtggacgcag attgataacg gtcagcttgc gccggacctg
     9481 actgaaatca ggacgtccat tacgaatgtc agcaatgaaa tcacgcaaac cgtcaataaa
     9541 aaactggaaa atcagagtgc tgcaatccag cagatacaga aagttcaggt tgatacaaat
     9601 aataacctga acagcatgtg ggctgtgaag ctgcaacaga tgaaggacgg acgcctttat
     9661 attgcgggta tcggtgccgg tattgagaat acgccagcag gaatgcagag tcaggtgctg
     9721 ctggcggcag acaggattgc gatgattaat cctgcgaatg gcaacacaaa gccgatgttt
     9781 gttggtcagg gcgatcagat attcatgaac gacgtgttcc tgaaacgcct gacggctccg
     9841 accattacca gcggcggtaa tcctccggca ttttccctga cacctggcgg acggctgacg
     9901 gcgaaaaatg ccgatatcag cggtaacgtg aacgcgaact ccgggacgct caacaacgtc
     9961 acgattaata agaactgtcg ggttctggga aaattgtccg cgaaccagat tgaaggcgat
    10021 ctcgttaaaa cagtgggcaa acctttccca cgggactccc gggcaccgga gaggtggcca
    10081 tcagggacca ttaccgtcag ggtttatgac gatcagccgt ttgatcggca aattgttatt
    10141 cccgcggtgg cgtttcgcgg tgctaaacat gagcggaaga ataacaatat ttattcgtca
    10201 tgccgcctga tagtgaagaa aaacggtgct gaaatttata accgaacgac cctggataat
    10261 acgctgatat atacgggtgt tattgatatg cctgccggtc acggtcacat gacgctggag
    10321 ttttctgtat cggcatggct ggtaaatggc tggtatccca cagcaagtat cagcgatttg
    10381 ctggttgttg tgatgaagaa atccacagca ggtatcagta tcagctgaat tttataaccc
    10441 agatacgggc accagaaatg gtgccttttt tattgcagaa aagcgagagg taattatgcg
    10501 taaactttat gctgccattt tgtccgcagc catctgtctg gctgtatccg gtgcgcctgc
    10561 atgggcgtct gaacatcagt ccacgctgag cgcggggtat cttcatgcct cgacgaacgc
    10621 tcccggcagt gatgatctga acgggattaa cgtgaaatac cgttatgagt ttacggacac
    10681 gctggggctg gtgacgtcat tcagctatgc caacgctgaa gatgagcaaa aaacgcatta
    10741 cagtgatacc cgctggcatg aggattccgt gcgtaaccgc tggttcagcg tgatggcggg
    10801 gctgtctgtg cgcgtgaatg aatggttcag cgcgtatgcg atggcgggcg tggcttacag
    10861 ccgtgtgtcg accttctccg gggattatct ccgcgtaact gacaacaagg ggaaaacgca
    10921 cgatgtgctg accgggagtg atgacaaccg ccacagcaac acgtctctgg cgtggggggc
    10981 tggcgtgcag tttaacccga ccgaatccgt ggccattgac cttgcttatg aaggttccgg
    11041 cagtggcgac tggcgcactg acggtttcat cgtgggtgtc ggttataagt tctgattagc
    11101 caggtaacac agtgttatga cagcccgctg gttcaggcgg gctttttgtg gggtgaatat
    11161 ggcagtaaag atttcaggtg tactgaaaga tggggcgggt aaacctgtcg taaattgtgc
    11221 gattgaactg cgggccagaa gaaccagtcc gaccgttgtg gcacacgttg ttgccacttg
    11281 cgtgacggac aataacggtg cttatgtgat tgaggctgag ccggggtatt acgaggttgc
    11341 gcttcactgt aacggctggc agccaacccg tgtcggggat attgatgtgg caccgactga
    11401 tgcaccgggg acactgaacg cgtttctgaa tgcaccaaag gatggtgatt tacgtccgga
    11461 ggtgatgaag cgctttgagg aaatggtggc gcaggcgcag cagagtgccg gggctgcagc
    11521 cggaaacgca cagcagacgg cgcaggatgt ggcggcagcc gcaacggccc gtgatgatgc
    11581 acaacgtttt gcggagaaag cccgacagga tgcaaccgtc acagctgagg acagaaaggc
    11641 cactgcggaa gatgtgacaa gcacaggagc aaatgcagcc gcagccggac agagcgcaca
    11701 ggatgccgca ggttatgccc gcgcagcaga acaggccaaa aatgacattg atgctgcgct
    11761 gaccggcact ctgaaaatgg ctaaccatct gtc
//
LOCUS       DAZPAB010000116        11719 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZPAB010000116 DAZPAB010000000
VERSION     DAZPAB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11719
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.116"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..852
                     /locus_tag="ACHUPG_002752"
     CDS             <1..852
                     /locus_tag="ACHUPG_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR0862300.1"
                     /translation="IRHIIIGNEGALCYITEVTVKIFKYTPENNLFYGYILEDMKTGF
                     NILREIMVEGYRPSIARLYDAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAE
                     IVARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHE
                     IYESVINRIRTEFPHADDITMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHN
                     PLNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTG
                     TIYPIEK"
     gene            874..2283
                     /locus_tag="ACHUPG_002753"
     CDS             874..2283
                     /locus_tag="ACHUPG_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0862301.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2261..3040
                     /locus_tag="ACHUPG_002754"
     CDS             2261..3040
                     /locus_tag="ACHUPG_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR0862302.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3037..3897
                     /locus_tag="ACHUPG_002755"
     CDS             3037..3897
                     /locus_tag="ACHUPG_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR0862303.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4045..4620)
                     /locus_tag="ACHUPG_002756"
                     /pseudo
     CDS             complement(4045..4620)
                     /locus_tag="ACHUPG_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4637..4897)
                     /locus_tag="ACHUPG_002757"
     CDS             complement(4637..4897)
                     /locus_tag="ACHUPG_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR0862304.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4888..6159)
                     /locus_tag="ACHUPG_002758"
     CDS             complement(4888..6159)
                     /locus_tag="ACHUPG_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0862305.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6237..6599)
                     /gene="queD"
                     /locus_tag="ACHUPG_002759"
     CDS             complement(6237..6599)
                     /gene="queD"
                     /locus_tag="ACHUPG_002759"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR0862306.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6918..8717
                     /gene="cysJ"
                     /locus_tag="ACHUPG_002760"
     CDS             6918..8717
                     /gene="cysJ"
                     /locus_tag="ACHUPG_002760"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR0862307.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8717..10429
                     /gene="cysI"
                     /locus_tag="ACHUPG_002761"
     CDS             8717..10429
                     /gene="cysI"
                     /locus_tag="ACHUPG_002761"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR0862308.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10503..11237
                     /gene="cysH"
                     /locus_tag="ACHUPG_002762"
     CDS             10503..11237
                     /gene="cysH"
                     /locus_tag="ACHUPG_002762"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR0862309.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11501..11653
                     /locus_tag="ACHUPG_002763"
     CDS             11501..11653
                     /locus_tag="ACHUPG_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR0862310.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
BASE COUNT         2812 a         2989 c         3235 g         2683 t
ORIGIN      
        1 attcgtcaca tcatcatcgg caacgaaggt gcattgtgct atatcactga agtaacagtg
       61 aaaatcttta aatacacccc ggaaaacaac ctcttctacg gctatatcct ggaagacatg
      121 aaaaccggct tcaacatcct gcgtgaaatc atggtggaag ggtatcgtcc gtcgatcgct
      181 cgtttgtatg acgctgaaga tggcacccaa cacttcaccc attttgccga cggaaaatgt
      241 gtgctgatct ttatggctga aggtaaccct cgcattgcga aggcgacggg cgaagggatt
      301 gcggaaatcg ttgcccgcta cccgcagtgc cagcgcgtgg acagcaaact gattgaaacc
      361 tggtttaaca acctgaactg gggaccggat aaagtggctg ccgaacgtgt gcagatcctc
      421 aaaaccggca acatgggctt taccaccgaa gtgtccggct gctggagctg catccacgaa
      481 atctacgaaa gcgttattaa ccgtattcgt actgagttcc cgcacgccga cgacatcacc
      541 atgctgggcg gtcactcctc tcatagctat cagaacggca ccaacatgta cttcgtctat
      601 gactacaacg ttgttgactg taagccggaa gaggaaatcg acaagtacca caatccgctc
      661 aacaaaatca tctgcgaaga aaccattcgc ctcggcggtt cgatggtgca ccaccatggc
      721 attggtaaac atcgcgttca ctggagcaag ctggaacacg gcagcgcgtg ggcgttgctg
      781 gaagggctga aaaagcagtt cgatcctaac ggcattatga atacgggtac tatctatccg
      841 attgaaaaat aatgtatcag gcagcgttcc gcgatgacgg ggcgttgcct tttcggcttc
      901 tcaggcgaga agccgttctt attaccggac aatgaagggg taaagatgaa cacttcaccg
      961 gtgcgaatgg atgatttacc gcttaaccgt tttcactgtc gaattgctac gctcactttc
     1021 ggcgcacacc tgaccgacgg ttatgttcta ggcgtcattg gttacgccat tattcaactt
     1081 acgcccgcca tgcaactgac gccgtttatg gcgggaatga tcggcggctc ggcgctcctt
     1141 ggtttgtttc ttggcagcct ggttcttggg tggatctccg accatattgg tcggcaaaaa
     1201 atcttcacct tcagcttttt gctgattacg ctcgcttcgt tcttgcaatt ttttgccacc
     1261 acgccagagc atcttattgg gctgcgcatt ttgatcggca ttggtctggg aggcgattac
     1321 tcagtaggtc acaccttgct ggctgaattt tccccgcgcc gccatcgcgg tattttgctg
     1381 ggcgcattca gcgtggtgtg gaccgtaggc tatgtgttgg caagtattgc cggacatcac
     1441 tttatttccg aaaacccgga ggcctggcgc tggttgctgg catcggcagc tctgcccgcg
     1501 ttgttgatta cgttattacg ctggggaacg ccagaatcgc cacgttggct actgtgccag
     1561 gggcgttttg cagaagctca cgctatcgtg catcgctatt ttggtcccca tgttttactg
     1621 ggcgatgaag tggtaacggc gacccataaa cacatcaaaa ccttgttctc ttcgcgttac
     1681 tggcggcgca cggcgtttaa cagcgtcttc tttgtctgcc tcgtaatccc atggtttgtg
     1741 atttatacct ggctgccaac tatcgcccag actattggtc tggaagatgc gctgactgcc
     1801 agcctgatgc ttaatgcgtt gttaattgtg ggcgcgctgc tgggattagt tctgacgcac
     1861 ctgctggcac atcgcaaatt tttgctggga agttttttgc tgctggcggc aacgctggta
     1921 gtaatggcct gtttgccttc cggcagttca ttaacgctgc tgctttttgt tctcttcagc
     1981 accaccattt cggcagtcag taatctggtg ggcattttgc ctgcggaaag ttttcctact
     2041 gacattcgct cgctgggagt cggttttgcc accgccatga gtcgacttgg cgcggcggta
     2101 agtactggcc tgctgccgtg ggtgctggcg cagtggggaa tgcaagtcac cttattgctc
     2161 ctagcgacag tgttgttggt tggttttgtt gtgacctggc tatgggcacc agaaactaaa
     2221 gccctcccgc tggtggcggc gggaaatgta ggaggtgcga atgaacattc tgttagcgtt
     2281 taaagccgaa ccggatgccg gaatgctggc ggaaaaagag tggcaggcgg cggctcaggg
     2341 taatagcgga ccggatgttt cgctactgcg aagtttactc ggtgctgatg aacaggccgc
     2401 tgccgcgctg ctgttagcgc agagaaaaaa cggtacgccg atgtctttaa ccgcgttgag
     2461 catgggggat gaacgggcgt tgcactggct gcgctatctc atggctcttg ggtttgagga
     2521 agctgtattg ctggagacgg cggcagatct gcgctttgcg ccggaatttg tcgctcgcca
     2581 tattgccgaa tggcagcgtc agaatccgct ggatctgata attaccggca gccaaagcag
     2641 cgaagggcag aatgggcaaa cgccattttt gctggcggaa atgctgggct ggccctgctt
     2701 cacccaggtg gagcgtttca ctctcgacgc gctgtttatc accctcgaac agcgtactga
     2761 acatgggttg cgctgttgcc gggttcgctt gcctgcggtg attgccgtgc gtcagtgcgg
     2821 tgaagtggcc ttgccagtac cgggaatgcg ccagcgaatg gctgcgggga aagcagaaat
     2881 tatccgtaaa actgttgctg ccgaaatgcc agcgatgcag tgtctgcaac tggcaagagc
     2941 agagcaacga cgcggagcga cgctgattga tgggcaaacg gtagcagaaa aagctcaaaa
     3001 gctatggcag gactatttac gccaaaggat gcagccatga atatcgcaat tgtgacaata
     3061 aatcaggaga atgccgcaat agcgagctgg ctggcggcgc aggatttcag cggttgtacg
     3121 ctggcgcact ggcaaataga acctcagccc gtagtggcgg aacaggtgct ggatgcgatg
     3181 gtggaacagt ggcagcgaac gcctgcggac gttgttctct ttccgccggg aacgtttggc
     3241 gatgaactgt cgacccggct ggcctggcgt ttacatggtg caagcatctg tcaggtgact
     3301 tcgttagata ttccaaccgt aagcatgcgc aaatcccact ggggaaatgc gttaacggcg
     3361 acgctgcaaa cagaaaaacg cccgttatgc ctttccctgg cgcgtcaggc gggagcagcg
     3421 aaaaacgcca cgttgccctc tggaatgcag caactgaaca ttgtcccagg tgcgcttccc
     3481 gactggctgg ttagcacaga ggatctgaaa aatgttagcc gcgacccatt agctgaggcc
     3541 agacgggtgc tggtggtggg acagggcgga gaggcagata accaggaaat cgttatgctg
     3601 gcagaaaagc tgggagccga agtgggctac agtcgggcgc gagtgatgaa cggtggcgtt
     3661 gatgctgaaa aagtgattgg tatttccgga catctgctgg cacctgaggt gtgcattgtt
     3721 gttggcgctt ccggcgcagc agcgttaatg gcgggcgtac gcaatagcaa gtttatggtg
     3781 gcgattaatc atgacgccag cgcagcggta ttttcacagg cagatattgg cgtggttgat
     3841 gactggaaag tggtgctgga ggcactggtg acaaacattc acgcagaata ccaataacgt
     3901 gctgccggat gatcctgcct acggttctgg actggggacc tgtaggcctg ataagacgcg
     3961 ttaagcgtcg catcaggcgc tgaatgtcgg atgtggcgtg cggccaacaa atcaaaacaa
     4021 attcaattgg ttacgattat cccgtcaaag caattttttc gctaacgtcc agactccggt
     4081 attcgtggtg gaaagcgcca cgacacccgc gttaatcgca ttacgcgcat cttcttcatc
     4141 gcataccagc ccgccagcaa tcagcggttg gcggattttt tccgtcaccc agcccagcac
     4201 tttgggcatg cagcctggca ggatctcaat acaatccgga ttcgattgcg caacttactt
     4261 atcaatgttg tgaaacgaaa tcgaatcaac aataaacagg tgatgaatac agaaaaaaac
     4321 ttccgctctt gccgctttta gcatcgatgc tttggtgctg ataattccat ccgcctcggt
     4381 caccagtttc aaaaactgaa tcaccacttc tttatttgac gcgccttcca gcaaatcaac
     4441 atgaataaaa gcatatttcc cggcgttctt aatcttttta acgatattac tgatggtgca
     4501 gatattgccg tacaacacgg aaataaactg gcattcagaa tcgatggcca gttgcaggct
     4561 ggcattatct ttaacggcag caatcaccgg attctggcgg agcaggtgta agaggggcat
     4621 aaattatcct tattatttat ccaaagcggt aggtgatgcc gaatccggaa ggcggatagc
     4681 gccactgttg tagtgtggat tcgtcgcaca gcaaacggca ggtgccacac tccaggcagc
     4741 cgcgatagtc aatacgtaag tcaccttcct gtgtgagaga aaaaagacct accggacagg
     4801 cgttaatcaa ccgttgtacc atctgacgct caacggagtc agccggaaca atgtgcggcg
     4861 catcagcaac gcgccagaga ttacgggcta cagacatcgc agactcctga taacatcgcc
     4921 cgccagatgc cacaggccat gacgacgtaa atgatgccag aataactggc gcagcggtgg
     4981 aacaggttta tcaccctgat cccataaatc gcgggaaata tcctgcatta acgcaggcca
     5041 cgcccgatac ctgccagggc gttgcaaaag cgccggaaca tgctgataac gttgtagaac
     5101 atcccatagc aggctgcgct ctacgttgtg atgataaagc gcaaacagat tttgcggctc
     5161 gcggtgctgg caggcgctta ttagcgtttg tgccgccgcc tgtgcgccag tcagcgccat
     5221 atccatgccg cgcacggaaa ttccggtatt gacgcaactg cgcaacgcat cgcccaccag
     5281 cagccagccg ttaccggcgt attgcaccgg catactgtgc aagccacctt ctggcaccag
     5341 atgcgcacca tactccagtg attccgtgtt tttgataagc gtgcgcactg ccggatgcgc
     5401 tttaaagcga gtcagcagct cgcttgccgg aacacgactt tgcgtaaggg aagagagcgg
     5461 gcaaacaatc cctaacgaga gcgtttgttg attagtataa agaaatgcgc cgccgggtaa
     5521 gtcatcacag attccgccgc tgaacagcaa cgctgcgcct tcgttattct ccagatgaaa
     5581 acgttcttca atagcggatg tttccagcga cagcacttct ttgatcccca acgccatcgc
     5641 ttcgccagca ggacgagtca ctaacccgtg acgttcagcc aggacgctgt tggcaccttc
     5701 tgccagcacc acataacggg cgcggagaat atcgtcacca caaataacgc cacagactct
     5761 gccgttttct tcatacagtg catccaccgt cgctccgggg atgcattcga caccttcttt
     5821 ttcggcttcg gcaaccagcc acggatcgaa ccgtgcacgt aatacattcc aggattcacc
     5881 gccgggctgt aagctggaaa acgtcgttgc gccatccggc gttaacaggg aaaggctttc
     5941 gtgagtgatg cgtcgttcaa gaggcgcggt cagatgaaat tgcgggagga gttccgcgag
     6001 tgcatgggta tataaccgcc tgccggaaag atttttgctg ccggggattt cagcgcgttc
     6061 gagtaacaaa acggataaat ccgctcgcgc gcagcgtaac gcgcaagcgg tgcctgcaat
     6121 accggcacca ataataataa tgtcgcagtc gtcttccatg tcatgcccca gatatccaga
     6181 acacaacacc ctaacatagc gttactaaag ggaaattgac cgccgacaca ctcttatcat
     6241 tcgccgcgat agatacaccc tgcggtgcag gtttctttta ccatcaccgc acttaacagc
     6301 ggcacaacgg gtttaacctg atcccaaatc cattttgcta aaacttcgct ggttggattt
     6361 tccagacctg gaatatcatt gagataatgg tgatcgaggc gctcataagt tggcttaaac
     6421 gccgctttca gttcagcgaa atcgataatc cagcccgtat gcggatcgac ttccccggta
     6481 atttccagtc gcaccataaa ggaatgcccg tgcaggcgac cacatttatg cccttccggg
     6541 acatgtggta agcggtgagc ggcttcgaag gtgaaatctt taaataacgt ggtggacatc
     6601 ataatttctc tacaggacga taaaaccgcc gtagagtacc ggaaaggctg ttttttcgca
     6661 ttatctaaaa cgactttgtt gcgcaaaatc gctgatttat cttaatgatt ggctaaattc
     6721 atttgttttt cattaggttg gttaatctat tttgttgtta aagactattg ctaaaacagg
     6781 ttagtcgatt tggttattag ttatcgctat cccgtcttta atccacaccg ttcaccccgt
     6841 taaccttacc ttctcttctg ttttatgggc gctgacaggg cgcagaaaca gctttgctta
     6901 ctggaacata acgacgcatg acgacacagg tcccaccttc cgcgttgctt ccgttgaacc
     6961 cggagcaact ggcacgcctt caggcggcca cgaccgattt aacgcctacc cagcttgcct
     7021 gggtttctgg ctatttctgg ggcgtgctca accagcagcc tgttgcgctt gcagtgacgc
     7081 cagcgcccgc cgcagaaatg ccgggtataa cgattatctc tgcttcgcaa accggcaatg
     7141 cgcgccgggt tgctgaagca ttacgcgatg atttattagc agcaaaactg aacgttaagc
     7201 tggtgaacgc gggcgactat aaattcaaac aaatcgccag cgaaaaactg ctcatcgtag
     7261 tgacgtcaac gcaaggggaa ggggaaccgc cggaagaagc cgtcgcgctg cataagttcc
     7321 tgttctccaa aaaagcgcca aagctggaaa acaccgcgtt tgccgtgttt agcctcggcg
     7381 atagctctta tgaatttttc tgccagtccg ggaaagattt tgacagcaag ctggcggaac
     7441 tggggggtga acgcctgctc gaccgtgtcg attctgatgt tgaataccag gctgctgcca
     7501 gcgagtggcg cgcccgcgtg gttgatgcgc ttaaatcgcg tgcgcctgtc gcggcacctt
     7561 cgcaatccgt cgctactggc gcggtaaatg aaatccacac cagcccgtac agcaaagacg
     7621 cgccgctggt ggctagcctc tctgttaacc agaaaattac tgggcgtaac tctgaaaaag
     7681 acgttcgcca tatcgaaatt gacttaggcg actcgggcct gcgttaccag ccgggtgacg
     7741 cgctgggcgt ctggtatcag aacgatccgg cactggtgaa agaacttgtc gaactgctgt
     7801 ggctgacagg cgatgaacct gtcactgtcg agggcaaaac gttgcctctg aacgaagcgc
     7861 tacagtggca cttcgaactg accgtcaaca ccgccaacat tgttgagaat tacgccacgc
     7921 ttacccgcag tgaaacactg ctgccgctgg tgggcgataa agcgaagtta cagcattacg
     7981 ccgcgacgac gctgattgtt gacatggtgc gtttctcccc ggcacagctt gatgccgaag
     8041 cgctaattaa tctgctgcgc ccgctgaccc cgcgtctcta ttccattgcc tcctcgcagg
     8101 cggaagtcga gaacgaagta cacgtcaccg ttggtgtggt gcgttacgac gtggaaggcc
     8161 gcgcccgtgc cggtggtgcc tccagcttcc tcgctgaccg cgtggaagaa gagggcgaag
     8221 tccgcgtatt tatcgaacat aacgataact tccgcctgcc cgctaacctg gaaaccccgg
     8281 tgattatgat tggcccaggc accggtattg cgccgttccg cgcctttatg cagcaacgcg
     8341 ccgctgacga agcgccaggt aaaaactggc tgttctttgg caatccgcat tttacggaag
     8401 acttcctgta tcaggtggag tggcagcgct acgtcaaaga gggtgtgctg acgcggatcg
     8461 atctcgcctg gtcgcgcgac caaaaagaaa aagtttacgt acaagataaa ctgcgcgaac
     8521 agggcgcgga gctgtggcgc tggatcaatg acggtgccca catttatgtc tgcggcgacg
     8581 ctaatcgcat ggcgaaagac gttgagcagg cacttctgga agtgattgcc gaatttggtg
     8641 gcatggacac cgaagcggcg gatgaatttt taagtgagct gcgcgtagag cgccgttatc
     8701 agcgagatgt ctactaatga gcgaaaaaca tccagggcct ttagtggtcg aaggaaaact
     8761 gacagacgcc gagcgcatga agcttgaaag caactacctg cgcggcacca ttgcggaaga
     8821 tttaaacgac ggtctgaccg gcggctttaa gggcgataac ttcctgctga tccgcttcca
     8881 cggcatgtat cagcaggatg accgcgacat ccgcgccgaa cgtgctgaac agaagctgga
     8941 gccgcgccac gcgatgctgt tgcgctgccg tctgccgggt ggagttatca ccaccaaaca
     9001 gtggcaggcg atcgataaat ttgccggtga aaacaccatc tatggcagca ttcgcctgac
     9061 caaccgccag acgtttcagt tccacggcat tctgaaaaag aacgtcaaac cggtgcacca
     9121 gatgctgcac tcggtcggtc ttgatgcgct ggcgactgct aacgacatga accgtaacgt
     9181 actctgcacc tcgaaccctt acgagtcgca gctgcacgca gaagcgtacg agtgggcgaa
     9241 gaagatttct gaacatctgc tgccgcgtac ccgcgcgtat gcggagatct ggctcgatca
     9301 ggaaaaagtc gccaccaccg atgaagaacc gatccttggt cagacttacc tgccgcgtaa
     9361 gttcaaaacc acggtagtga tcccgccgca gaacgatatt gatctgcacg ccaacgacat
     9421 gaacttcgtg gcgatcgcgg aaaacggcaa gctggtgggc ttcaacctgc tggtgggcgg
     9481 tgggctttct atcgaacacg gtaacaagaa aacctacgcc cgcacggcga gtgagtttgg
     9541 ctatctgccg ctagagcata cgctagcggt ggcggaagcc gtcgtgacaa ctcagcgtga
     9601 ctggggtaac cgaaccgatc gtaaaaatgc caaaaccaaa tacacactgg agcgcgtggg
     9661 ggttgagacg tttaaagcgg aagtggagcg tcgcgcgggg atcaaatttg aaccgatccg
     9721 cccgtatgaa tttacaggac gcggcgatcg tattggctgg gttaagggca ttgatgataa
     9781 ctggcacctg acgctgttta tcgaaaatgg tcgcatcctt gattatccgg ggcgtccgct
     9841 gaaaactggc ctgctggaga tcgcgaagat ccacaaaggt gatttccgca ttacggcgaa
     9901 ccagaatctg atcatcgccg gtgtgccgga aagcgagaaa gcgaagatcg agaaaattgc
     9961 caaagagagc gggttaatga atgccgtcac gccgcagcgt gaaaactcga tggcctgcgt
    10021 gtcattcccg acttgcccgc tggcgatggc ggaagcggag cgtttcctgc cgtcttttat
    10081 cgacaacatc gataatttaa tggtgaaaca tggtgtcagc gatgagcata tcgtgatgcg
    10141 tgtaacaggc tgcccgaacg gttgtggtcg cgcgatgctg gcggaagtgg gcctggtggg
    10201 taaagcaccg ggtcgctaca acctgcatct tggcggcaac cgcattggga cacgtatccc
    10261 acggatgtat aaagaaaaca tcaccgagcc ggaaatcctg gcgtcgcttg atgaactgat
    10321 agggcgctgg gcgaaagagc gcgaagcggg tgaaggcttc ggcgacttta cggtgcgtgc
    10381 gggcatcatt cgcccggtgc tcgatccggc gcgcgatttg tgggattaac catcagcccg
    10441 gtcttgtagg cctgataaga cgcgtgagcg tcgcatcagg caaggcaaac agtgaggaat
    10501 ctatgtccaa actcgatcta aacgccctga acgaactgcc gaaggtagat cgcattctgg
    10561 cgctggcaga aactaacgcc gaactggaaa aactggacgc tgaaggccgc gttgcctggg
    10621 cgctggataa tctgcccggt gaatatgtac tttcttccag ctttggcatt caggcggcgg
    10681 tgagcctgca tctggtgaat cagattcacc cggatattcc ggtgatcctc accgacaccg
    10741 gctatctgtt cccggaaacc taccgcttta ttgacgagtt aacggacaaa ctcaagctca
    10801 acctgaaagt gtaccgcgct accgaaagcg ccgcctggca ggaagcacgc tacggcaaac
    10861 tgtgggaaca gggcgttgaa ggcattgaaa agtacaatga catcaacaaa gtcgaaccga
    10921 tgaaccgggc gctgaaagaa ctgaatgcgc aaacgtggtt tgctggcctg cggcgtgaac
    10981 aatccggcag ccgtgccaat ttaccggtgc tggcaattca gcgtggcgta tttaaagtgc
    11041 tgccgattat cgactgggat aaccgaacta tttatcagta cctgcaaaaa catggcctga
    11101 aatatcaccc attatgggac gaaggatatt tatcggtggg cgatacccat acaacccgta
    11161 aatgggaacc cggcatggcg gaagaagaaa cgcgtttctt tggcttaaaa agggaatgtg
    11221 gattacacga agggtaaata ttgccggata aattaaacgc ctctgtcaga aatgatgggg
    11281 gcgtttttat tttgcgagca gcattccggc atttaaaggt gaacatcgca gggaaaatta
    11341 acaggtatag aatggcgttg ggtgcttgag gctgtctggc gttaagcatt cgcgaggttc
    11401 cagatggaca aaagccccag gcgatatttc tatcaacctg aggctcgcgt tcgaacgtag
    11461 caattcggat gttagtctct tctttgcagg gaggcaagac atgctgacaa aatatgccct
    11521 tgtggcaatc atcgtactgt gttgtacagt actgggattc acgttgatgg taggtgactc
    11581 gttatgtgag ttgagtatca gagaacgtgg tatggagttt aaggcagttc tcgcttacga
    11641 atcgaagaag tagccaccac gcgggggtaa aaccccgcgt actgaattgt gatggtttgg
    11701 tctcctgcac ccttttttt
//
LOCUS       DAZPAB010000117        11559 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZPAB010000117 DAZPAB010000000
VERSION     DAZPAB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.117"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            27..1190
                     /locus_tag="ACHUPG_002764"
     CDS             27..1190
                     /locus_tag="ACHUPG_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFR0862311.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            1191..2417
                     /locus_tag="ACHUPG_002765"
     CDS             1191..2417
                     /locus_tag="ACHUPG_002765"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR0862312.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            2414..3292
                     /locus_tag="ACHUPG_002766"
     CDS             2414..3292
                     /locus_tag="ACHUPG_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR0862313.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            3303..3656
                     /locus_tag="ACHUPG_002767"
     CDS             3303..3656
                     /locus_tag="ACHUPG_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR0862314.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            3668..4972
                     /locus_tag="ACHUPG_002768"
     CDS             3668..4972
                     /locus_tag="ACHUPG_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR0862315.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            4984..6069
                     /locus_tag="ACHUPG_002769"
     CDS             4984..6069
                     /locus_tag="ACHUPG_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR0862316.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(6229..6960)
                     /gene="frlR"
                     /locus_tag="ACHUPG_002770"
                     /gene_synonym="yhfR"
     CDS             complement(6229..6960)
                     /gene="frlR"
                     /locus_tag="ACHUPG_002770"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR0862317.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(7060..7845)
                     /gene="frlD"
                     /locus_tag="ACHUPG_002771"
     CDS             complement(7060..7845)
                     /gene="frlD"
                     /locus_tag="ACHUPG_002771"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR0862318.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(7842..8672)
                     /gene="frlC"
                     /locus_tag="ACHUPG_002772"
     CDS             complement(7842..8672)
                     /gene="frlC"
                     /locus_tag="ACHUPG_002772"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFR0862319.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(8722..9744)
                     /gene="frlB"
                     /locus_tag="ACHUPG_002773"
     CDS             complement(8722..9744)
                     /gene="frlB"
                     /locus_tag="ACHUPG_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR0862320.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(9765..11102)
                     /gene="frlA"
                     /locus_tag="ACHUPG_002774"
                     /pseudo
     CDS             complement(9765..11102)
                     /gene="frlA"
                     /locus_tag="ACHUPG_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            complement(11397..>11559)
                     /locus_tag="ACHUPG_002775"
     CDS             complement(11397..>11559)
                     /locus_tag="ACHUPG_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR0862321.1"
                     /translation="KFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKI
                     IGGCGPTAQ"
BASE COUNT         2627 a         2945 c         3256 g         2731 t
ORIGIN      
        1 tcaaggaaca gaaacgaggc aacaagatgt ttgtagaagc attgaaacgc cagaacccgg
       61 cgctgatttc cgccgcacta agtttgtggc agcagggcaa aatcgccccg gacagctggg
      121 tgatcgacgt ggatcagata ctggaaaacg gtaagcggct cattgagacg gcgcggcttt
      181 acggcattga actgtatctg atgaccaagc aatttggtcg taatccgtgg ctggcggaaa
      241 aattgctggc attaggctac agcggcattg tggcggtgga ttacaaagag gcgcgagtca
      301 tgcgccgcgc tggtttgcca gtggcgcatc aggggcatct ggtacaaatc ccttgtcatc
      361 aggttgctga cgccgttgaa cagggcaccg acatcatcac cgtgtttact ctcgacaaag
      421 cgcgggaagt ttctgcggca gcggttaagg ccggacgggt ccagcctgtg ctgctcaaag
      481 tttatagcga cgatgatttt ctttatccgg ggcaggagag cggttttgta cagcattcgc
      541 tgcacgaggt gattgctgaa attaagaaac tgccggggct gcatttagcc ggactaaccc
      601 atttcccttg cctgctttgg gatgaggctg ctgggaaagt tttgccaaca ccgaatcttc
      661 acacgctggt acaagcacgg gatcaactgg cgaaatctgg tattgcgatt gagcaactga
      721 acgtaccttc agcgaccagc tgcacttcgc tgccattgct ggcggaatac ggtgcgactc
      781 atgccgaacc cggtcatgcg ctgaccggta ctattccggc aaaccagcag ggcgatcagc
      841 ctgaacgtat cgcgatgctc tggttaagtg aaatctccca tcatttccgt ggcgacagct
      901 actgctacgg cggcggttac taccgtcgtg gtcatgcgca acatgcgctg gtgtttacgc
      961 cagaaaatca aaagattact gaaaccaatc tcaaaactgt ggatgacagc agtatcgact
     1021 acaccctgcc gctggcaggc gagtatccgg tgagcagtgc agtggtgctc tgttttcgca
     1081 cgcagatttt tgtcacccgt agcgatgtgg tgctggtgtc cggtattcag cgcggcgaac
     1141 cagaaatagt tggtcgttat gacagcctcg gcaaccctct ggaggcataa atggcgcgat
     1201 ttgtggtgtt agtgattgat agctttggcg taggggcaat gaaagatgtc acgctggtgc
     1261 gcccgcaaga tgccggggcg aatacctgtg gtcacatcct gagccagttg ccgcatttgc
     1321 agctaccaac gctggagaag ctggggctaa tcaacacatt gggttatgcg ccaggcgata
     1381 tgcagccgtc agattccgca acctggggcg tggcagagct gcaacatgaa ggtggcgata
     1441 cctttatggg gcatcaggaa attttaggca cgcgcccgtt accgccgctg cggatgcctt
     1501 ttcgcgatgt gattgaccgt gttgagcagg cattagtttc cgctggctgg caggtggagc
     1561 gccgtggcga tgatctgcaa tttctgtcgg tcaatcaggc ggttgcgatt ggcgataatc
     1621 tcgaggcgga tttaggccaa gtctataaca ttaccgccaa tctctctgtg atctcttttg
     1681 acgacgcaat caaaattggt cgtatcgtgc gtgaccaggt acaggtcggt cgggtcatta
     1741 catttggcgg cctgttaacc aacagtcagc gtattctcga tgccgcagaa agcaaagaag
     1801 ggcgctttat tggtattaat gcgccgcgtt ctggcgctta tgacaacggc ttccaggtcg
     1861 tgcatatggg ctacggtgtt gatgaaaaag tgcaggtgcc gcaaaaactg tatgaagcgg
     1921 gcgtgccaac cgtgctggtg ggtaaggtgg cagatatcgt cagcaatcct tatggtgtga
     1981 gctggcaaaa cctggtggat agccagcgga ttatggatat caccctcgac gaatttaaca
     2041 cccatccgac ggcgtttatt tgcatcaata tccaggaaac cgacctcgct ggtcatgcag
     2101 aagacgtcgc acgttatgcc gaacgtttgc aggtcgttga ccgtaacctt gcccggcttg
     2161 ttgaggcgat gcagccagat gattgcctgg tcgtgatggc ggatcacggt aacgatccga
     2221 ccattggtca cagccaccat acccgtgaag tggtgccagt gctggtttat cagcaagggc
     2281 tggtcgccac gcagctcggt gtgcgcacca cgctttctga tgtgggagct accgtgtgtg
     2341 aatttttccg tgcgccaccg ccacaaaatg gtcgctcttt tctttcctcc ctccggtttg
     2401 caggagacac cctatgagtt ttgatccgac gggttacacc ctggcccatg agcatctgca
     2461 tattgatctc tccggcttta aaaacaacgt ggactgccgc cttgatcagt atgcgttcat
     2521 ttgccaggag atgaacgacc tgatgacccg gggtgtacgt aatgtgattg agatgaccaa
     2581 ccgttacatg gggcgcaatg cgcaatttat gctcgatgta atgcgcgaaa ccgggatcaa
     2641 cgtggtggcc tgtactggtt attaccagga tgcgtttttc ccggaacatg tggtgacccg
     2701 cagcgtgcag gaactggcgc aggagatggt cgatgaaatt gaacagggta tcgatggcac
     2761 ggagctgaaa gccgggatca tcgcggagat cggctccagc gaaggaaaga ttacgccgct
     2821 ggaagagaag gtatttattg ccgctgcgct ggcgcataac cagaccggac gcccgatctc
     2881 cacgcatacg tcgttcagca ccatggggct ggaacaactg gctctgttgc aagcccacgg
     2941 agttgatctt tcgcgcgtca ccgttggtca ctgcgatctg aaagacaacc tcgacaacat
     3001 tttgaagatg atcgatctcg gcgcgtacgt gcagttcgac accattggta agaacagtta
     3061 ctacccggac gaaaagcgca ttgcgatgct ccacgcgcta cgtgaccgtg ggttgctgaa
     3121 ccgcgtcatg ctgtcgatgg atattacgcg ccgctcccat ttaaaagcca atggtggtta
     3181 tggctatgac tatttattga ccacctttat tccgcaattg cgccagtcag gattcagtca
     3241 ggccgatgtg gatgtgatgt tacgtgaaaa tccctctcaa tttttccaat aaggacagac
     3301 tcatgaaaaa gattggcgtt gcaggcttac agcgtgagca gattaaaaaa accattgaag
     3361 cgacggctcc tggctgtttt gaagttttca ttcacaacga catggaagcg gcaatgaagg
     3421 tgaaatccgg gcaactagat tattacattg gcgcgtgtaa taccggtgcg ggcgcggcat
     3481 tgtcgattgc catcgcggtg attggctata acaaaagttg caccattgcc aaaccaggca
     3541 ttaaagcgaa agacgagcat atcgccaaaa tgatcgctga agggaaagtg gcgtttggcc
     3601 tttccgttga gcacgtcgaa cacgcgattc cgatgttgat taaccatctg aaataaaagg
     3661 cacgactatg gatctgtata ttcagattat cgtggtggcg tgcctgacgg gtatgacatc
     3721 gcttctggag catcgctcgg cggctgtttt tcatgacggc atccacccga tcctgccgca
     3781 actgattgaa ggctatatga accgtcgcga ggcggggagt atcgcttttg gtctgagcat
     3841 tggttttgtg gcctcggtgg ggatctcttt taccctgaaa accgggctgc tcaacgcatg
     3901 gttactcttt cttcctaccg atatcctcgg cgtactggcg ataaacagcc tgatggcgtt
     3961 tggtcttggc gctatctggg gcgtgttgat ccttacttgc ctgttgccag ttaaccagct
     4021 gctgaccgcg ctgccggtgg atgtattagg tagcctcggg gaattaagct cgccggtggt
     4081 ttctgctttt gcactgttcc cgctggtggc gattttctac cagtttggct ggaagcaaag
     4141 tctggtcgcc gccgtggttg ttctgatgac ccgtgtggta gtcgtgcgct atttcccaca
     4201 tcttaaccct gaatccatcg aaatctttat tggcatggtg atgctgctgg ggatcgcgat
     4261 aactcacgac ctgcgtcatc gtgatgaaaa tgacatcgat gccagcgggc tttcggtgtt
     4321 tgaagagcgc acgtcgcgga ttatcaaaaa cttaccttat atcgccatcg tgggagcatt
     4381 gattgccgcc gttgccagta tgaagatttt tgctggtagc gaagtttcga tcttcacact
     4441 ggagaaagcg tattccgcag gcgtgacgcc ggaacaatcg caaacgctga ttaatcaggc
     4501 ggctctggca gaatttatgc gcggactggg gtttgtgccg ttgattgcca ccaccgcctt
     4561 agcaacgggt gtgtatgcag ttgcgggctt tacctttgtt tatgcggtgg gctatctctc
     4621 gccgaatccg atggttgcag cggtattagg cgcagtggtt atttcggcgg aagtcttgct
     4681 gcttcgttcg atcggcaaat ggctgggacg ctacccgtcg gtgcgtaatg cgtcggataa
     4741 catccgtaac gccatgaata tgctgatgga agtggcgctg ctggtcggtt cgattttcgc
     4801 agcaattaag atggcgggtt ataccggatt ctctatcgcg gttgccattt acttcctcaa
     4861 cgaatccctg ggccgtccgg tacagaaaat ggcggcaccg gtcgtggcag taatgatcac
     4921 cggtattctg ctgaatgttc tttactggct tggcctgttc gttccggctt aaggaaggca
     4981 cctatgaaga cgtttccttt gcaaagcctg actcttgctg aagcgcagca aaagcagttt
     5041 gcgctggtgg acactatttg ccgccatttt ccaggcagcg agtttcttgc tggcggtgat
     5101 ttagggttaa ctccaagtct gaatcaaccg cgtattaccc aacgggtgga gcaggtgctg
     5161 gctgatgcat ttcacgcaca ggccgcggcg ctggtgcagg gcgcggggac tggcgcgatt
     5221 cgcgccgggc tggcggcttt gctcaaaccg gggcagcgtc ttctggtgca tgacgcgcct
     5281 gtttacccga cgacacgggt tattattgag cagatggggc tgacgcttat tactgctgat
     5341 ttcaatgacc tgtcggcact gaagcaggtc gtcgacgagc aacaacctga tgccgcgctg
     5401 gtgcagcata cgcgccagca gccgcaggac agctatgtgc tggcagatgt gctggcaacg
     5461 ttgcgcgcgg caggtgttcc agcgttaacc gatgataact atgcggtgat gaaggtagcg
     5521 cgaatcggct gtgaatgcgg cgcgaatgtc tcgacattct cctgcttcaa gctgtttggg
     5581 ccggagggcg ttggtgcagt ggttggcgat gccgatgtta ttagtcggat tcgcgccacg
     5641 ctttactccg gcggtagcca gatccagggc tcacaggcgc tggaagtctt gcgtggtctg
     5701 gtgttcgcgc cagtgatgca tgcggtgcag gcgggagtat ccgaacggtt gctggctttg
     5761 cttaacggcg gcgcggtggc ggaagtgaaa agcgccgtca ttgccaatgc gcagtcgaag
     5821 gtgttgattg tcgagtttca tcagctgatt gccgccaggg tgctggaaga agcgcaaaag
     5881 cgtggcgcgt tgccttaccc ggtcggtgca gagtcgaaat atgaaatccc gccgctcttc
     5941 tatcgacttt caggaacgtt tcgccaggcg aatccacaac tcgaacattg tgcgattcgc
     6001 attaacccga atcgcagcgg tgaagagacg gtgctgcgga ttttgcgtga gagtattgcg
     6061 agtatttaat gctggatagc accgttcggc aatcaagcta aattccgcac tgaacggtcc
     6121 cctcgcccct ttggggagag ggttagggtg aggggaacgt gcgggatgta aaattcagtg
     6181 cgccagcatc gccggatgcg atgctggcgc atcttatccg gcctacggtt aatgtctttt
     6241 attatcaatc gttaaggtga tgcgattggc gcggcagaag agttcggaga cgtgcaccgg
     6301 tttgtcattc tgatcaaagg cgattttgct gatgcgaaac aacggttcgc ccagttcgca
     6361 tttcagccat tttgcctgcg ggcgggtggc ggcaaagata tcgatggtct ttttgtcgct
     6421 gaccactcgc gtgtcaaaac gctcctgaaa taactgatag gtggagcttc cttcgacgta
     6481 aatctcatca aagtcaggat aacgcgacag cgggatccat gaactatcaa taaacagcgg
     6541 ctctttatcg agatacatca cccggcagag atggaacact tcgctgtttc cggggatgtt
     6601 cagcttttcg caaaacggcg cggcgctgac ccgttcctgt tcgatcactt tctctttcgt
     6661 cgacttgcct tgtgagacgc caaaatcggt aaaaccactg acagtaagca gggcgttttc
     6721 aactttctgg ctttgtacaa aggtgccttt tccctgccag cggatcagta cgccgtctgc
     6781 cactaagtcg ctgatggctt tgcgaatggt aatgcggctg acgttatatt gtgtacaaag
     6841 ctcgttttcg gtagggatct gttgcccggc ctggtaaacc ccctgcgcga tatcatccag
     6901 cagccgctgg cggacggtag cgtagaggag ttgatgagag tagcggtccg tagctgacat
     6961 aatcgaccct gaaataaaga atgaattact cggattatag ggtcgttttc cggcaagtaa
     7021 cagcacgttg gcgtgaagat gctcacgcca acgttatacc taccaggcac cgtggtactg
     7081 aatggttttc gccgcgcacg ccgttccctg cgccatcgcc tgcggtaatg tcatccccgc
     7141 agaccagccg caaaggaatc cggcaatgaa cgaatctccg gcacccatgg tgtcgataac
     7201 cgtcaccggt tcaggagcct gacgccagaa ctgcgcgcca tcccaggcaa tgctgccgtt
     7261 ttcacccagc gtgacaatca ctgttcctgc gccacgggca acaatcgctt tcatcttcag
     7321 acgcagcgct tcgtcttctt gcggtgcgga ggcaaaggca aaatcgagat gcggcaccag
     7381 tgtctgccag agcgggctgt cccacttgtc ggagaagtcg aaagcggtaa gtttgcccgc
     7441 agcgtgcagc tgtgggaatg cgtcttccgc atgtccccag attgccgcgt gcacaatgtc
     7501 atactgcgcc agccaggcgt aatcctcttc actcagggca aagtcggcca tcacgccttc
     7561 ggtgtagtcg ccaaaaacgc gatcattgtc gtgcagttcc acctgagttt gtgcggtaac
     7621 gccgtgtttc gtatggacat ggctgatatc gacgcccatg cgggcgagat cctgcttcag
     7681 ctttgtgccg tagtcatcgt cacccaccca ggtaatgcat cccggctgta tgccgtagcg
     7741 agtgcagtac accgccacat tgaccgcatt accgccagaa aacgctttat tcagttgcgg
     7801 gtagatatcg acgcagttat cgccgattgt cgccagggtt ttcatctctc atcctccggc
     7861 agcagcgcgc gaaagcgttc cagcgcctgg cgggcataga gtctgggctc gttcatatac
     7921 atcgtcacca gctccaccgt agagtaaccc tcatagcccc gatcaataat atcgcgcatc
     7981 agttcccgca gcggcatttt gccttctcca ggaatgtaat gcgtgtcgct ggccccgtcg
     8041 ctgtcgacaa tatgcagatg acgtaattta tcgcccagtt tgtcgaaata actcatcacc
     8101 ggttccgcct ggacatacgg cgcgcaaatg tcgaccatgc tgaacaggcg cggcgaaggc
     8161 accagcgcca gcgcatgaag cacatcatta gcattacaca cgacgttcga ttcatacggc
     8221 gttaacggtt cgaggatcag atccatgccg atgttttccg cgtactcaca cagttcgctt
     8281 aagttctctg ccagccgccc ccagataacg ttaggcggcg tgagatagcc cgcgtgggcc
     8341 gcagaaatca gcgtataacc cgcgttcatc tcttttgcca tatctatcgc cagcttgatc
     8401 atgtccaggc tttcacgacg catatgttca tcgcccagca tcatgttata cggatagccg
     8461 ttggtttctg gcgtgtaacc gataatcggc atctgatacg tctgcgccag cgccttgatt
     8521 tgtttgatgc cgcccgcttt taagtccggc gcgaacgcgt gcgggcgacc gccccaaatt
     8581 tcgatgccgt cgtaacccag ctcgcttgca tcacgaaatg cgtgttcaat aggcagccgc
     8641 tggtggccgc aggtaaacat acctgttttc atcgggtaac tccttgcgta aatccggccc
     8701 gttgccgggc cgggagaggg attaatattc caccagacca ccgtaatagc ggcgttcatc
     8761 cgggttgtga tctttgtaaa tagacaggta gtagcagagc cactccattg gcacgaacat
     8821 caggaacggt gccagccacg ggtgcagccc ttgcgaaatt tcggcgtaat cgatgacgat
     8881 cacgttgtca gtacgctgtt taacaaagtt aatggcgcgt tcggtggtgt ggcgactttc
     8941 atcattgccg agcaggaaca ggaacggaac gcccggttcg acaatctcca gcgggccatg
     9001 gcggaactct ccgctctcaa tcacgcagcc gtgcgtccag gtaaattcca tcagcgttac
     9061 aatgccttct ttgtaaccca gcggacgcag cggacccgca gcaacggtat aaatcatcgg
     9121 ccactggctg gccagttcac caagctggcg gcctttttct tcccaggtgc gtaccagatg
     9181 accgagcgca ttcggcaact gcttgagatc gtttttgatc ttgccgattt ccgcgttcgg
     9241 cgcgaggcgg gtgatcatct ccagcaccac gctgtagcag agcagcaggt gaatttccca
     9301 gatacagtcg gcctgataat caatgctaaa ttccgccgcc gaggtaatcg ggctgtccgc
     9361 gcgtttggtg aacgccgcag tgagtgctcc gcaggcccgg cccagctcca gcgctttgat
     9421 tacctcttcc gttttaccgt agtcagaaac gccaatcacc gcgcaacgat cgtcgaggcg
     9481 atacggggtg ttatcgcaga actcccagcc ggaaatggcg tagacctgaa gatcggaaaa
     9541 gcgatccgcc agatgtttcg ccgtttgcgc cgcgttgagt ggcgagccgc aggcaacgaa
     9601 ataaatacga tcaatgtcgc gcttcaccat ctcttccacg atggcgtgca ccagcggaac
     9661 gtcatggctg agaacttttt ccacttcctg aaccatattt tcggtgacca gaaagtccac
     9721 ggtgctttta tcaatatcca acattttatt ttcttcagat aacattacga caaagcgttg
     9781 agctggcggc tacgcttcgc ccagaaggcg taagcaggca gaccagtagc aataacgatg
     9841 gcggcgcaga taaggccggg aatcggtgcc cagacaaagg ttgaggcgac cagaatgagg
     9901 cttgacgcaa tggcgagggt ggtcatcagc ccgaaagccg gagtacgcca cagcggtttg
     9961 taatcgtcgc gtttacgaca ccagatgatg gagccgaagg tgagggtatt tttgaaacac
    10021 atcaccaggg tgaaataacc cagcaggctg gtgagatcgg aaacgaagat gaagaagatc
    10081 cccagcgccc cttgcaggat gatggagaca tccggcgtgt tgtatttcgg atgcgcatgg
    10141 ccgaagcatt taaagaacag gttgtctttc gccatcgcgt attccagccg cggctggtac
    10201 atcacgcagc tggaaagcga accaagaatg acgatcatcg ccgtgatggc aacaaagagc
    10261 ccagcggtgc tgccgagtgc ggggatctag gtcagggcgt cggaaatcgg cgtttcagaa
    10321 ttggcgagtt tgtcgaaggg catcaggccg gaaatcacca gcgccagcag ggtgtagagc
    10381 accagaacca gcagacagga accaatcagc gctcgtggca tggtttttcc ggggttttta
    10441 atttcgccgg tcatataaca gatagaggcc atgccggtat acgaccaact ggtggcagag
    10501 atccccgcca gcagcgccat aaagctgccc gttgcgccaa tcgcagtggt ggtaagggcg
    10561 gcaaaattct ccgctttaaa ccagaagatc ccaaggccaa tgacgatagt gaacgggata
    10621 attttggcga tggtaattag cgtctgaaac gctgcgccgc cttcaacgga gcgcaggtgt
    10681 agcaacataa aggcgataat taatccggcg gcgataaatt taccgagcaa cggatcgata
    10741 ggcgttaaaa agccaagatt gctgacaatc gccagcgcca taatcgacaa tgacggcgca
    10801 tcgttggccc agaagctggc ccagccggag aggaaagcca gcggtcggct tccggcattt
    10861 ttcagataaa cataatctgc gccattttcc ggataagcgg tggatagttc cgcatagacg
    10921 cacatttgcg ggatcacaat taacccgcca atgacaaacg cgagcaccgt aagccacggc
    10981 gtgcccgctg cttttgccac ttcacccaca gatacaaaaa taccggagcc gacggttgtc
    11041 ccgacggcga ttgcaagaac ggcccaaaat ccgagcttgc gttggagttc ctggcttccc
    11101 atagcgatac ctttttcttg tccaatacgt tgtaggggag agtttgcagc catttaaaag
    11161 gtaacatgac aatgcatgat gaatataaca acatattatg ttatgtgatg tggaggaaga
    11221 tcacaaaatt tcgcacagga tcgcgctgtg gctaatggat gtagttataa aattgaattt
    11281 aaagtgaaaa tatttttacg ggcgggggca agaaggacat agaaacaaat acgccctcgg
    11341 aaaatccaga gggcgtcggg caattaaacc ggtgttagcc gatttctgtc agagacttac
    11401 tgtgcagtag gaccgcaacc gccaatgatt ttagaaatag aaattgccgg gtgcagcagg
    11461 tagtcgtaag agcagttctt atcacggttt tcaatatgac cagtacatgc agttaacgta
    11521 gccagtactg cacctaccag tgcaacttta ataaatttg
//
LOCUS       DAZPAB010000118        11490 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZPAB010000118 DAZPAB010000000
VERSION     DAZPAB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.118"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(103..2859)
                     /gene="barA"
                     /locus_tag="ACHUPG_002776"
     CDS             complement(103..2859)
                     /gene="barA"
                     /locus_tag="ACHUPG_002776"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR0862322.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            2916..4217
                     /gene="rlmD"
                     /locus_tag="ACHUPG_002777"
     CDS             2916..4217
                     /gene="rlmD"
                     /locus_tag="ACHUPG_002777"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR0862323.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            4265..6499
                     /gene="relA"
                     /locus_tag="ACHUPG_002778"
     CDS             4265..6499
                     /gene="relA"
                     /locus_tag="ACHUPG_002778"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR0862324.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            6595..7386
                     /gene="mazG"
                     /locus_tag="ACHUPG_002779"
     CDS             6595..7386
                     /gene="mazG"
                     /locus_tag="ACHUPG_002779"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR0862325.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            7614..9251
                     /gene="pyrG"
                     /locus_tag="ACHUPG_002780"
     CDS             7614..9251
                     /gene="pyrG"
                     /locus_tag="ACHUPG_002780"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR0862326.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            9339..10637
                     /gene="eno"
                     /locus_tag="ACHUPG_002781"
     CDS             9339..10637
                     /gene="eno"
                     /locus_tag="ACHUPG_002781"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR0862327.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(10697..11490)
                     /locus_tag="ACHUPG_002782"
                     /pseudo
     CDS             complement(10697..11490)
                     /locus_tag="ACHUPG_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="YgcG family protein"
BASE COUNT         2928 a         2767 c         3006 g         2789 t
ORIGIN      
        1 cggataaggc gttaacgccg catccggcat aaacacaggc actttgtcac caatctgaaa
       61 ccagcgtcat aaaaagccga ttgctactcg acaagacgtc cgttacccga gaattttgct
      121 ggcttcgcgc gcgacattat ccatctcgtc caacagttcc agcagctccg gctccaaatc
      181 ttcttcttta gtaccgctac gtagctgttg ttcgatcagc tggcagagat tcttcatacg
      241 tggtacgccg ctatagccgc aactgccatg cagtttatga atcaaatcaa ccaggccttc
      301 cgggttttct ccagccagct gttcctcaac tttgttgcga acttcaggca ggaaatcgag
      361 taacatttgc agcatatcgc gcgctaaatc ggtttttcct gctgcctggc gtagtgccag
      421 ttgccagtcg agggtcgcat tcgggttcac cacaatttcg ttgacttcgg gcgtcacgac
      481 gcgagacgaa ataccgctgc caggcttgta gcgcaacaac aaattatgca atcgctcttc
      541 ttcaatcggt ttcgccagat aatcgctcat ccctgcgcca agcagcttct ctttttgccc
      601 ggccattgca tgcgccgtta ccgcgataac cggcgtttgt cgctgatgcg ggagttggtg
      661 gatgagttcg caggcccgaa tgccatccat gtccggcatt tgaatatcca ttaagatcaa
      721 atcgaacggc atctgtttcg cccgttcaac tgcctgatgc ccgctatcgc aaagttccac
      781 atgttgcacc atatcttcca gcaatgcgcc gataagtttc aggttagcgg ggttgtcatc
      841 aaccgccatg actgtcattg ccagcttact ttcatcggtt acaggcaaaa gcgtgttttg
      901 tttgtgatga caaaattccg tcagggcagg caacaggcgc gtaggtgtta atggtttcag
      961 cagacacgcg ccgataccat cttgcttgag tttttcagca ttaacttgtg catggcaagg
     1021 aagtgccagc atcaggaaat cggtcatcga taccgctttc gctaatcgct catgttgcat
     1081 tgttagcggc tcgcggaagg tcaccgcgat gcctaacaac atcatgtcgt aatgcgcggg
     1141 aggcagcgcg gagaacgttg ggctataaac cacttccagc ggcgtttcac ttaaaatatc
     1201 cagcgtgcat tgcgctgctg cggagtttgg ttcgacatag gccaggcgct tacctgcgag
     1261 gcactggatg gatggccctt cgataataat gttcgggttc agatcgagat taatgtggaa
     1321 ccagaaagtt gaaccgcgat tcggctggct atggaacgaa atatcgccgc ccatttcatt
     1381 aaccagtttt tgtgtaatca ccagccccag cccggtgcca ccatgacggc gggaaatact
     1441 ggcatcagcc tgtcggaagg cctggaataa gcgcgattga tcgcgttcag gaataccaat
     1501 gccggtatcc cgaatctgca cttcaatctg cactttggta ttactcagcg cacgtttttc
     1561 taccagaata tcaatgttgc cattctcggt gaatttaatt gcattcccca ccaggttagt
     1621 gatgatttgc tgtaatcgca gtgggtcgcc gatcacgtta tcaggcacgt cgcttttaat
     1681 attgagcgtc agttccaacc ctttatcgtg agaagaatgt gccagcagag taacgacttc
     1741 atccagcgtg ctgcgtagtg ggaatggaat actttccaga atcagcttac ctgcttccag
     1801 tttcgagaag tcgagaacat cattaataat tgccagcaaa ttatttgccg aacgttcaat
     1861 cgtattcagg tgatcgcgct gcgttggtgt taattctgtt ttcagcgtca ggcgggtaaa
     1921 gccaataaca ccattcagtg gtgtacgcag ctcgtgtgac atatttgcca gaaactcgga
     1981 tttaatacgc gccgcttcct gggcgcgctt tttcgccaga tccaactcaa cgttctgaat
     2041 ttccatctgc tccagcgttt cacgcagatc ggacgtcgcc tggtcgatat tgtgctgcat
     2101 ctcttcgtga taagcagcca gcgacattgc catcgagttg ataccgtttt tcagcatatc
     2161 cagctcgccg agcataaatc cttccacccg gctgtcgagt tgcccgcgac ggatgcggtc
     2221 gacggtattc accatgttgc gaatcggacc ggttacatcg cgcattaagc gccagccaaa
     2281 aataagcgca ataccgatac aaaacagcat catcacgcag gaaataaaga tctctttata
     2341 ttgctgcaag cgaaccgatt taagatccag ctcaagcgca atatatccca acatattttg
     2401 actatttttg gcatcgctac tgggcgattc gtcgggggag taactctcag aaataatcgg
     2461 cgtgcggagg atcataatat cgccgtcacg agtgacagtg agctggcgag gaaacggcac
     2521 gttgctgccg agctgcattg atgagggatc aagatgaaaa ttggaggtga caaagagtcg
     2581 gttattttca tcataaaccg aaatcgcgcg aacaatatcg gaatggcgac gatgcagtac
     2641 gcttattaac tgaccgatag attcgcgatt ttgcaggctc atgccatatt cagtagaaac
     2701 tgcaagcggc tcaataatgc tagcaccggc atcttccagt tgacgctgca agtcgttata
     2761 gcgatgcacg acgaaaaaga tactcagcaa taaaccaata aggacggtcg gtgccaggat
     2821 cagaatcatc atgcgtgcgc gcaggctgta gttggtcatg gagttccgtt atgggacaat
     2881 taaggtcaca ctgttaaatt gagaaagtct cgccaatggc gcaattctac tctgcaaaac
     2941 gacgcacgac gacgcgtcag atcataaccg tttcagtcaa cgacctcgac tcttttggtc
     3001 agggcgtggc gcgacataac ggcaaaacgt tatttatccc cggattattg ccgcaggaaa
     3061 acgcggaagt tgctgttact gaagataaaa aacagtatgc ccgcgctaaa gtcgtacgcc
     3121 ggttaagcga tagcccggaa cgcgaaacgc cacgctgtcc tcattttggc gtatgcggcg
     3181 gctgtcagca acaacacgcc agcgtggatt tacagcagcg aagcaaaagt gcggcactcg
     3241 cccgattaat gaaacacgat gtctctgaag tgatcgccga tgttccctgg ggctatcgcc
     3301 gccgcgcgcg tttaagtctg aactacttac cgaaaacaca gcaacttcag atggggtttc
     3361 gcaaagcggg ctccagtgac attgtcgacg tcaaacaatg ccccatttta gcgccccaac
     3421 ttgaagcatt gctgcccaaa gtcagggcat gtctgggcag cttacaagct atgcgccatc
     3481 ttggtcatgt tgaactggta caggcaacca gcggcacgct gatgattttg cgccataccg
     3541 caccgctaag ttcggcagat cgcgaaaaac tggaacgctt ttcgcattct gaaggcctgg
     3601 atctgtatct cgcccccgat agtgagatac tcgaaaccgt ctctggtgag atgccctggt
     3661 atgactcaaa cgggttgcgc ttaactttta gcccgcgcga ttttattcag gtcaatgcgg
     3721 gtgtgaacca aaaaatggta gcgcgtgcgt tggaatggct ggatgtgcaa cctgaagatc
     3781 gcgtactgga tctgttctgc ggtatgggca actttacact gccattggcg acacaagctg
     3841 ccagtgtggt cggtgtagaa ggtgttcctg cgctggtgga aaaaggccag cagaatgcgc
     3901 gtcttaatgg cttacagaat gtgacgtttt atcacgaaaa tcttgaagaa gatgtcacaa
     3961 agcagccgtg ggcgaaaaac ggcttcgata aagtgttgct ggacccggcg cgagcaggtg
     4021 ccgcaggtgt tatgcagcaa attataaaac tggaacctat tcgtatagtt tatgtatcct
     4081 gtaaccctgc aacgctggct cgggatagcg aagcgttatt aaaagcagga tataccattg
     4141 cgcgactggc gatgctggat atgttcccac acacgggaca tctggaatcg atggtacttt
     4201 tctcgcgcgt taaatagtta cgatttgccg atttcggcag gtctggtccc taaaggagag
     4261 gacgatggtt gcggtaagaa gtgcacatat caataaggct ggtgaatttg atccggaaaa
     4321 atggatcgca agtctgggta ttaccagcca gaagtcgtgt gagtgcttag ccgaaacctg
     4381 ggcgtattgt ctgcaacaga cgcaggggca tccggatgcc agtctgttat tgtggcgtgg
     4441 tgttgagatg gtggagatcc tctcgacatt aagtatggac attgacacgc tgcgggcggc
     4501 gctgctgttc cctctggctg atgccaacgt agtcagcgaa gatgtgctgc gtgagagcgt
     4561 cggtaagtcg gttgttaacc ttattcacgg cgtgcgtgat atggcggcga tccgccagct
     4621 gaaagcgacg cacactgatt ctgtttcctc cgaacaggtc gataacgttc gccggatgtt
     4681 attggcgatg gtcgatgatt ttcgctgcgt agtcatcaaa ctggcggagc gtattgctca
     4741 tctgcgcgaa gtaaaagatg cgccggaaga tgaacgtgta ctggcggcaa aagagtgcac
     4801 caatatctac gcgccgttgg cgaaccgtct tgggattggg caactgaaat gggagctgga
     4861 agattactgc ttccgttatc tccacccgac cgaatacaaa cgcatcgcaa aactgctgca
     4921 tgaacgccgt ctcgaccgcg aacactatat cgaagagttt gtcggccatc tgcgcgctga
     4981 gatgaaagct gaaggcgtta aagctgaagt gtatggtcgt ccgaaacaca tctacagcat
     5041 ctggcgcaaa atgcagaaaa agaacctcgc cttcgatgag ctgtttgatg tgcgtgcggt
     5101 acgtattgtc gccgagcgtt tacaggattg ttatgccgca ctggggatag tgcacactca
     5161 ctatcgccac ctgccggatg agtttgacga ttacgtcgct aacccgaaac caaacggtta
     5221 tcagtctatt cataccgtgg ttctggggcc gggtggaaaa accgttgaga tccaaatccg
     5281 caccaaacag atgcatgaag atgcggagtt gggtgttgct gcgcactgga aatataaaga
     5341 gggcgcggca gctggcggcg cacgttcggg acatgaagac cggattgcct ggctgcgtaa
     5401 actgattgcg tggcaggaag agatggctga ttccggcgaa atgctcgacg aagtacgtag
     5461 tcaggtgttt gacgaccggg tgtatgtctt tacgccgaaa ggtgatgtcg ttgatttgcc
     5521 tgcgggatca acgccgctgg acttcgctta ccacatccac agtgatgtcg gacaccgctg
     5581 catcggggca aaaattggcg ggcgcattgt gccgttcacc taccagctgc aaatgggcga
     5641 ccagattgaa attatcaccc agaaacagcc gaaccccagc cgtgactggt taaacccaaa
     5701 cctcggttac gtcacaacca gccgtgggcg ttcgaaaatt cacgcctggt tccgtaaaca
     5761 ggaccgtgac aaaaacattc tggctgggcg gcaaatcctt gacgacgagc tggaacatct
     5821 ggggatcagc ctgaaagaag cagaaaaaca tctgctgccg cgttacaact tcaatgatgt
     5881 cgacgagttg ctggcggcga ttggtggcgg ggatatccgt ctcaatcaga tggtgaactt
     5941 cctgcaatcg caatttaata agccgagtgc cgaagagcag gacgccgccg cgctgaaaca
     6001 gcttcagcaa aaaagctaca caccgcaaaa ccgcagtaaa gataacggtc gtgtggtggt
     6061 tgaaggtgtt ggtaacctga tgcaccacat cgcgcgctgc tgccagccga ttcctggaga
     6121 tgagattgtc ggcttcatta cccagggacg cggtatttca gtacaccgcg ccgattgcga
     6181 acaactggcg gaactgcgct cccatgcgcc agaacgcatt gttgacgcgg tatggggtga
     6241 gagctactcc gccggatatt cgctggtggt ccgcgtggtg gctaatgatc gtagtgggtt
     6301 gttacgtgat atcacgacca ttctcgccaa cgagaaggtg aacgtgcttg gcgttgccag
     6361 ccgtagcgac accaaacagc aactggcgac catcgacatg accattgaga tttacaacct
     6421 gcaagtgctg gggcgcgtgc tgggtaaact caaccaggtg ccggatgtta tcgacgcgcg
     6481 tcggttgcac gggagttagt cttacatagc tggatatggc gtcagccgta tccagcgata
     6541 tcttttactc gaaggtatta cacctgacag acccgcaatt ttcaggacaa ttcaatgaat
     6601 caaatcgacc gtttgctcac tattatgcag cgcctgcgcg atccggaaaa cggctgcccg
     6661 tgggataaag agcagacatt tgccaccatt gcgccttaca cccttgaaga aacctacgaa
     6721 gtgctggacg ccatcgcccg tgaagatttt gacgatcttc gcggtgaact gggcgatctg
     6781 ctattccagg tggtgtttta cgcgcaaatg gctcaggaag aagggcgctt tgactttaat
     6841 gatatttgcg ctgctattag cgataaatta gagcgtcgcc atccgcatgt ttttgctgat
     6901 agttctgccg aaaacagtag tgaagtgctt gcccgttggg agcaaatcaa aaccgaagag
     6961 cgcgcgcaga aagcgcagca ttcggcgctg gacgatattc ctcgtagttt accggcttta
     7021 atgcgggcgc aaaaaatcca gaaacgttgc gccaacgttg gcttcgactg gacgacgctt
     7081 ggcccggtgg ttgataaagt ctacgaagag atcgacgagg tgatgtacga ggcgcggcag
     7141 gctgttgtcg accaggctaa actggaggag gaaatggggg acctgctgtt tgccacggtt
     7201 aatctggctc gccatttagg gacgaaagca gaaatcgcat tgcaaaaagt gaacgaaaaa
     7261 ttcgagcgtc gttttcgcga agtggagcgt attgttgccg cgcgtggact ggaaatgaca
     7321 ggtgttgacc tcgaaacaat ggaagaagtc tggcaacagg taaaacggca ggaaattgat
     7381 ctctaaggga attacgcggt caagcgccat ttgtgtcatt ttttaaatga caagcgcttg
     7441 atttgcgtca aaaacattta ccccaaaggg gctattttct cactcctgat ttcaatagtg
     7501 cgctggcgaa gaggagggat aatgaaagtt tgtggcacag gtcatgttcg ggtatactgc
     7561 tttcccgtct tggttattcc atcgtctttt caacctaact tctcaggttc agcatgacaa
     7621 cgaactatat ttttgtgacc ggcggggtcg tatcctctct gggtaaaggc attgccgcag
     7681 cctccctcgc agccattctt gaagcccgtg gcctcaatgt gaccatcatg aaactggatc
     7741 cttacatcaa cgtcgatcca ggtactatga gcccaatcca acacggggaa gtgttcgtta
     7801 ctgaagacgg cgctgaaacc gacttggacc tggggcacta cgagcgtttc attcgtacca
     7861 aaatgagccg ccgcaacaac ttcaccacgg gtcgtatcta ctctgacgtt ctgcgtaaag
     7921 aacgccgcgg tgactacctc ggcgcaaccg tgcaggttat tccgcacatc actaacgcaa
     7981 tcaaagagcg cgtgctggaa ggtggcgaag gtcatgacgt agtactggta gaaatcggcg
     8041 gtacagtagg tgatatcgaa tccttgccgt tcctcgaagc tattcgccag atggctgttg
     8101 aaattggccg tgagcacact ctgtttatgc acctgacgct ggtgccgtac atggcagcgt
     8161 ctggtgaagt caaaaccaaa ccgactcagc actctgtaaa agagctgctc tccatcggta
     8221 tccagcctga catcctgatt tgtcgttcag atcgcgctgt accggcgaac gaacgtgcga
     8281 agattgcatt gttctgtaat gttccggaaa aagcggttat ttctctgaaa gacgtcgatt
     8341 ccatctataa aattccgggc ctgttgaaat ctcaggggct ggacgattat atttgtaaac
     8401 gattcagctt aaactgcccg gaagcaaatc tgtccgaatg ggaacaggtt atcttcgaag
     8461 aagcgaatcc ggtaagtgaa gtcaccatcg gtatggtcgg caagtacatt gaactgccgg
     8521 atgcttataa atcagtgatc gaagcactga aacacggtgg gctgaagaat cgtgtcagcg
     8581 tcaacatcaa actgatcgat tcacaagatg ttgaaacgcg cggcgttgaa atccttaaag
     8641 gtctggacgc aatcctcgta cctggcggtt tcggctatcg tggcgtagaa ggcatgatta
     8701 cgaccgcgcg ttttgcgcgt gagaacaata ttccttatct gggcatttgc ctgggtatgc
     8761 aggtggcgtt aattgattac gctcgccatg ttgccaacat ggagaacgcc aactctacag
     8821 aatttgtgcc agactgtaag tacccggttg tggcgctgat taccgagtgg cgcgatgaaa
     8881 acggcaacgt tgaagttcgt agcgagaaga gcgatctcgg cggtaccatg cgtctcggcg
     8941 cacagcagtg ccagttggtt gacgatagcc tggttcgcca gctatacaat gcgccgacaa
     9001 ttgttgagcg tcatcgtcac cgttacgaag tcaacaacat gctgttgaaa cagattgaag
     9061 atgcaggtct gcgcgttgcg ggccgttccg gggatgatca gttggtcgag atcatcgaag
     9121 ttccgaatca cccgtggttc gtggcttgcc agttccatcc ggagtttact tctactccac
     9181 gtgatggtca cccgctgttt gcaggctttg tgaaagccgc cagcgagttc cagaaacgtc
     9241 aggcgaagta agtaaaaaag ttagaacggc aacgcgtacc ttgggtacgc gttgtttgtc
     9301 tggagtttca gtttaactag tgacttgagg aaaacctaat gtccaaaatc gtaaaaatca
     9361 tcggtcgtga aatcatcgac tcccgtggta acccgactgt tgaagccgaa gtacatctgg
     9421 agggtggttt cgtcggtatg gcagctgctc cgtcaggtgc ttctactggt tcccgtgaag
     9481 ctctggaact gcgcgatggc gacaaatccc gtttcctggg taaaggcgta accaaagctg
     9541 ttgctgcggt aaacggcccg atcgctcagg cgctgattgg caaagatgct aaagatcagg
     9601 ctggcattga caagatcatg atcgacctgg acggcaccga aaacaaatcc aaattcggcg
     9661 cgaacgcaat cctggctgta tctctggcta acgccaaagc tgctgcagct gctaaaggta
     9721 tgccgctgta cgagcacatc gctgaactga acggcactcc gggcaaatac tccatgccgg
     9781 ttccgatgat gaacatcatc aacggtggtg agcacgctga caacaacgtt gatatccagg
     9841 aattcatgat tcagccggtt ggcgcgaaaa ctgtgaaaga agccatccgc atgggttctg
     9901 aagttttcca tcacctggca aaagttctga aagcgaaagg catgaacact gctgttggtg
     9961 acgaaggtgg ctatgcgccg aacctgggtt ccaacgctga agctctggct gttatcgctg
    10021 aagctgttaa agctgctggt tatgaactgg gcaaagacat cactctggcg atggactgcg
    10081 cagcttctga attctacaaa gatggtaaat acgttctggc tggcgaaggc aacaaagcgt
    10141 tcacctctga agaattcact cacttcctgg aagaactgac caaacagtac ccgatcgttt
    10201 ctatcgaaga cggtctggac gaatctgact gggacggttt cgcataccag accaaagttc
    10261 tgggcgacaa aatccagctg gttggtgacg acctgttcgt aaccaacacc aagatcctga
    10321 aagaaggtat cgaaaaaggt atcgctaact ccatcctgat caaattcaac cagatcggtt
    10381 ctctgaccga aactctggct gcaatcaaga tggcgaaaga tgctggctac actgcagtta
    10441 tctctcaccg ttctggtgaa actgaagatg ctaccatcgc tgatctggct gttggtactg
    10501 ctgcaggcca gatcaaaact ggttctatga gccgttctga ccgtgttgct aaatacaacc
    10561 agctgattcg tatcgaagaa gctctgggcg aaaaagcacc gtacaacggt cgtaaagaga
    10621 tcaaaggcca ggcataagtc tgactttatc tgatttgata atgccagcct tcgggctggc
    10681 attttttatg gtggagttac cagcggccgg atgcgccgcc acttgagcct ccagcggaac
    10741 cgctgttgtc tgaatgataa cttctaccag atgctctttt ctgaagtaca cataaacagg
    10801 taaagaccat aaatatggtg aaagtaaaaa agaaggaaaa tacagcaatg gaaaaagttt
    10861 tatcggagaa caggaataaa tctaaaagat ggattgctga aatacagact ccacttttac
    10921 atgcgcgaca aaaattactg ccttgatgaa aaaaccagaa agggaagaac atcactgcaa
    10981 atacccaaaa aatagcaaag taataatgat cagtggaaga cgttgattca ctttctgaag
    11041 gattagccgg atattggtgt tgtgaagtga gttggttatt caaagcgatt atagctaact
    11101 ctaatccctt agctaatttt tgttgtttaa atgcgggtat catgttgcta cggatgatat
    11161 ctccagccag agcatcggtt accttttctt ccagcccata acctacctgg atgcggacag
    11221 tgcgatccga ctaggcaaca acgatcagta tcccatcatt acgtttggca tctcctaaac
    11281 gccaattgtc aaaaactctt gtagcatatt gttcaattgt ctcatctttg gttgtggaaa
    11341 caattaatac agcaatttgc acttgggtat tattgaaaat agattctaac ttgtgcgtca
    11401 gttctttctt ttcttgtgag tttaagctac tcgtaagacc cgtaacttgt tgctgtaatt
    11461 gtgggacaat agttggttgg gctgcaacaa
//
LOCUS       DAZPAB010000119        11465 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZPAB010000119 DAZPAB010000000
VERSION     DAZPAB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.119"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            50..322
                     /gene="rcnR"
                     /locus_tag="ACHUPG_002783"
     CDS             50..322
                     /gene="rcnR"
                     /locus_tag="ACHUPG_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFR0862328.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      414..510
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            545..1333
                     /gene="thiM"
                     /locus_tag="ACHUPG_002784"
     CDS             545..1333
                     /gene="thiM"
                     /locus_tag="ACHUPG_002784"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR0862329.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            1330..2130
                     /gene="thiD"
                     /locus_tag="ACHUPG_002785"
     CDS             1330..2130
                     /gene="thiD"
                     /locus_tag="ACHUPG_002785"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR0862330.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            2195..3012
                     /locus_tag="ACHUPG_002786"
                     /pseudo
     CDS             2195..3012
                     /locus_tag="ACHUPG_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
     gene            3064..3810
                     /locus_tag="ACHUPG_002787"
     CDS             3064..3810
                     /locus_tag="ACHUPG_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR0862331.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(3784..4749)
                     /locus_tag="ACHUPG_002788"
     CDS             complement(3784..4749)
                     /locus_tag="ACHUPG_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR0862332.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(4746..5749)
                     /locus_tag="ACHUPG_002789"
                     /pseudo
     CDS             complement(4746..5749)
                     /locus_tag="ACHUPG_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            5789..6841
                     /gene="fbaB"
                     /locus_tag="ACHUPG_002790"
     CDS             5789..6841
                     /gene="fbaB"
                     /locus_tag="ACHUPG_002790"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR0862333.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            7149..8003
                     /gene="gatY"
                     /locus_tag="ACHUPG_002791"
     CDS             7149..8003
                     /gene="gatY"
                     /locus_tag="ACHUPG_002791"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR0862334.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            8032..9294
                     /gene="gatZ"
                     /locus_tag="ACHUPG_002792"
     CDS             8032..9294
                     /gene="gatZ"
                     /locus_tag="ACHUPG_002792"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFR0862335.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            9304..9756
                     /gene="gatA"
                     /locus_tag="ACHUPG_002793"
     CDS             9304..9756
                     /gene="gatA"
                     /locus_tag="ACHUPG_002793"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR0862336.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            9787..10071
                     /gene="gatB"
                     /locus_tag="ACHUPG_002794"
     CDS             9787..10071
                     /gene="gatB"
                     /locus_tag="ACHUPG_002794"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR0862337.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            10075..11430
                     /locus_tag="ACHUPG_002795"
     CDS             10075..11430
                     /locus_tag="ACHUPG_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR0862338.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         2774 a         2809 c         3040 g         2842 t
ORIGIN      
        1 agattaatag tgctatgatt tttcatattc ttgttaacca ggtgttgcca tgtctcatac
       61 aatccgtgat aaacagaaac tgaaagcgcg tgccagtaag attcagggcc aggtcgtggc
      121 gctcaagaaa atgctcgacg agccgcacga atgcgctgca gttttacaac agattgctgc
      181 tatccgtgga gcggtaaacg gtctgatgcg ggaagtgatt aaaggtcatc tgacggaaca
      241 catcgttcac cagggggatg agctaaaacg tgaagaagat ctggatgtcg ttctgaaggt
      301 gctggattca tatatcaaat aatttattaa cgcgattgta aaactgccgt ttttcctcgt
      361 ttacaacggg ggcgctggac attaccatcc tcctctgcga tttatcatcg caaccaaacg
      421 actcggggtg cccttctgcg tgaaggctga gaaatacccg tatcacctga tctggataat
      481 gccagcgtag ggaagtcacg gaccaccagg tcattgcttc ttcacgttat ggcaggagca
      541 aactatgcaa gtcgacctgc tgagttcagc gcaatctgcg cacgcgttac acctttttca
      601 ccaacattcc cctcttgtgc actgcatgac caatgatgtg gtgcaaacct ttaccgccaa
      661 taccttgctg gcgctcggtg catcgccagc gatggttatc gaaaccgaag aggccagtca
      721 gtttgcggct atcgccagtg ccttgttgat taacgttggc acactgacgc agccacgcgc
      781 acaggcgatg cgtgctgccg ttgagcaagc aaaaagctct caaacacgct ggacgcttga
      841 tccagtagcg gttggtgcgc tcgattatcg ccgccatttt tgtcatgaac ttttatcttt
      901 taaaccggca gcgatacgtg gtaatgcttc ggaaatcatg gcattagctg gcattgctaa
      961 tggcggacgg ggagtggata ccactgacgc cgcagctaac gcgatacccg ctgcacaaac
     1021 actggcacgg gaaactggcg caatcgtcgt ggtcactggc gaggtggatt atgttaccga
     1081 tggacatcgt atcattggta ttcacggtgg cgatccgtta atgaccaaag tggtaggaac
     1141 tggctgtgca ttatcggcgg ttgtcgctgc ctgctgtgcg ttaccaggcg atacgctgga
     1201 aaatgtcgca tctgcctgtc actggatgaa acaagccgga gaacgcgcag tcgccagaag
     1261 cgaggggcca ggcagttttg ttccacattt ccttgatgcg ctctggcaat tgacgcagga
     1321 ggtgcaggca tgaaacgaat taacgctctg acgattgccg gtactgatcc gagtggtggt
     1381 gcggggattc aggccgatct taaaaccttc tcggcacttg gcgcttatgg ttgctcagtt
     1441 attactgcac tggtggcgca aaatacccgt ggcgtacagt cggtgtatcg cattgagcct
     1501 gattttgtcg ccgcccagct cgattcggtg ttcagcgatg tgcgaatcga taccactaaa
     1561 atcggtatgt tggcggaaac cgatattgtt gaagcggtgg cagaacggtt gcaacgttat
     1621 cagatccaaa acgtggtact cgacaccgtt atgctggcaa aaagcggcga cccgctgctt
     1681 tcaccttcgg cggttgctac gctgcgcagt cgattattgc cacaggtttc attaataacg
     1741 ccaaacttgc ccgaagctgc cgccttgctc gacgcgccac acgcgcgcac cgaacaggaa
     1801 atgctggaac aagggcgatc gctgttggcg atgggctgtg gcgcagtgct aatgaaaggt
     1861 ggtcatctgg atgatgagca aagcccggac tggctgttta cccgcgaggg tgaacaacgg
     1921 tttaccgcac cgcgcattat gaccaaaaac acccacggca ctggttgtac actctctgcg
     1981 gcgttggctg cactacgccc gcgccataca aactgggctg acaccgtaca ggaggcaaaa
     2041 atctggcttt catcggcgtt agcccaggcc gacacgctgg aagttggtca cggtattggt
     2101 ccggttcacc acttccacgc ctggtggtga gcaccgtact atagctgctt tggttattgt
     2161 cagcctttgg cactgacatt cagggatgaa accaatgcag ctaagaatta ccagtcgaaa
     2221 aaaactcacc gctctattat gcgctcttgg gctaatttcc atcgttgcaa tttatccgcg
     2281 tcagacggtg aattttttct attcgacagc agttcagatt acagactaca tccacttcta
     2341 cggttatcgc ccggttaaat ctttcgctat tcgcattcct gccagttaca ccattcacgg
     2401 attagatgtt tcgcgctggc aggagcgtat cgactggcag cgggtggcaa aaatgcgcga
     2461 caacgatatc cgcttacagt ttgcttttat taaggctacg gaaggcgaaa agctggtgga
     2521 cccctatttt tcgcgtaact ggcaattaag ccgcgaaaat ggcctgctgc gcggggcgta
     2581 tcattatttc tccccgtcgg tatccgcttc agttcaggcg cgattatttc tgcaaacggt
     2641 ggatttctca caaggcgatt tccctgccgt gctggacgta gaagaacggg gaaaattatc
     2701 ggcaaaagaa ttacgcaagc gggtaagtca gtggctaaaa atggtcgaaa aacgcacggg
     2761 aaaaaagccg attatttact caggagccgt tttttatcac accaatctgg cgggctattt
     2821 caatgaatat ccgtggtggg tggctcacta ttatcaacgt cgtccggaca atgacggcat
     2881 ggcctggcgc ttctggcagc attccgaccg tggacaggta gatggcatca atggtccggt
     2941 ggattttaat gtgtttaatg gcacggggat gagttgcagg cattcgttga tgggattaaa
     3001 gaaacgcctt aaataagaag cagtgatata ctcccaccag actccctggc aggaaaaaga
     3061 caaatggaac aagcgcatac ccagctgatt gcccaactga acgagcgtat tttagcggcg
     3121 gataacacgc cgctttatat taagtttgcc gaaacggtaa aaaatgccgt gcgcagcggg
     3181 gtgctggagc atggcaatat tttgcccggt gagcgtgatt taagtcagtt aaccggcgtt
     3241 tcgcgcatta cggtgcgcaa ggcgatgcag gcgctggaag aagagggcgt ggtgacgcgt
     3301 tcgcgcggtt acggcacgca gatcaacaac atcttcgaat actcgttgaa agaagcgaga
     3361 ggtttttctc agcaggtggt attgcgcggg aaaaagcccg atacgctatg ggttaataag
     3421 cgtgtcgtga aatgtcccga agaagttgcg cagcagctgg cggccgaagc gggaagtgat
     3481 gtctttttgc ttaagcgtat tcgctatgtc gatgaagaag cggtatcgat tgaggaatcg
     3541 tgggttccgg cgcatttaat tcatgatgtt gatgccatcg gaatttctct ttatgactat
     3601 ttccgcagcc agcacattta cccacaacgt acgcgttccc gcgttagcgc ccggatgccg
     3661 gatgccgagt ttcagtcaca tattcagtta gatagcaaaa taccggtgct ggtgatcaag
     3721 caagtggcgc ttgaccaaca gcaacggcct attgagtaca gcattagcca ctgtcgcagc
     3781 gatctatacg tttttgtgtg cgaggagtag ttcctcgcgc gttggcgcac aatcaccgcc
     3841 ccgatgcccg acaacccacg acgccactgc attgcccagt aatacggcat ccgccagtgg
     3901 cagaccagag gccagcccgg caagtacgcc acctgcatga ctgtcgcccg ccccaatggt
     3961 gtctacaact tgcgttggaa atgccggaat gcagccagaa gcgtcgttgc tgaaatacca
     4021 tgcgccttct ttatcgagac gaacgatcaa cggcgcggca aatttctcct gccattgctt
     4081 gccaagtgtt gttatctctg cggataaagc aaaacgttcg gcggcaatct cagcctcttg
     4141 acgattgagt gacactaaag gtcgacaggc catgatccgc gccagtaatg cgtccgggat
     4201 atcgccaata cgtgggcaaa aatcgataaa cggcgtcacg tcttgcagct tttccagcca
     4261 ttccactaac aattcgccgc agggcgaggc cagttgataa ccggaaaaat agagcaggtt
     4321 gccaggcgca acggttaatc gtgccaacca ttggcgattc cattgatttt ccacaccgct
     4381 gaatgacata aaagtgcgtt cgccatccgg ctcaaccagt gccagacacc agccgttatc
     4441 gctttcggcg ttatcgatca ggctgattaa cccctctttc gccatccggt tgcgaataat
     4501 ctccgcccac acgccctgac cgagcggcaa ggcattaccc gcttcgatgc cgaggcgctt
     4561 taacgccacg gcaatattca gtgcgcagcc gccaacgtta acgctctgct gtttcagttc
     4621 gatatcgcac ccacgccagg ggagggcata agcgtcggcg atcacgtcaa tgaccgccgc
     4681 gccgacgacc atcaccggct gacgcgtggt taattcaggc agcagtgtgt gtaatcgagc
     4741 gccgctcata ccgcctcccg ttgttgacga tatttcgcca gcgctgtggc atagcggttg
     4801 aaatcaagct gatttaccgc atccagctcc gcctttaacg caggatcgac agcgttaatg
     4861 ccatgcaacg cgccgcaaat cgccgtcgcc atagcaccta tggtgtctgt gtcgccgcca
     4921 agattggcgc acaggacggc gcagcggttt gggtcggttt gtgccagttc aactagcgca
     4981 atggcgcacg gaacggactc aatagtgctg gtgcctgcgc caacgacctg gtaaagctgt
     5041 tcgctggcgg attcggtgcc gtcggcatta cgcacaattt tcagcgcaat ctccagacgt
     5101 gccgccagtg aggcgctgaa cgtggtggtg cgtttttgct gtgcatgtcg cgcaattgaa
     5161 ggcaatgaat caacaatcgc tgaccagcgt tctccgtcaa tggcacgaga aatcgcccat
     5221 gcgatgacta ccgcgcctgc aaccgccaga tcggatttat gggtcgggct ggacgccagc
     5281 gccacatcat caataaagga atcaacatca cgcgctggaa gcaaacaacc taatggcgag
     5341 acgcgcatcg ctgcgccgtt ggttacgccg ttgttttcca gttcagcaac gggtttaccg
     5401 tcgcgaatgg cgttaagcgc aatcttcgag gtcggaccta gtacgttttt gttaaaggcg
     5461 tcgaaacgca acgcccagtc gagaatatta cgcccaatca gattcggatc gatcttgcct
     5521 tcacattcca gtaacgcatc cgccagacac aggccatcga ggtatcgtcg gtgaattcgg
     5581 cgcggttaaa ataacaggcc gcgttattct cctttggtcc aggcagaaaa cggtcaatcc
     5641 agccaaagtg tgctttaacg cggctgcgtg gccaaagctc ggagggcatc cccatcgcat
     5701 cccctaacgc ctgcccataa agagcaccga gaatacgttc tgttttcatt taacttcccc
     5761 ttgaatcgga atttacggga gcatagtaat gacagatatt gcgcagttgc ttggcaaaga
     5821 cgccgacaac cttttacagc accgttgtat gactattcct tctgaccagc tttatctccc
     5881 cggacatgac tacgtagacc gcgtgatgat tgacaataat cgcccgccag cggtgttacg
     5941 taatatgcag acgttgtaca acaccgggcg tctggctggc acaggatatc tttctattct
     6001 gccggttgac cagggcgttg agcactctgc cggagcttca tttgctgcta acccgctcta
     6061 ctttgacccg aaaaacattg ttgaactggc gatcgaagcg ggctgtaact gtgtggcatc
     6121 aacttacggc gtgttggtgt cggtatcgcg gcgctatgcg catcgcattc catttctcgt
     6181 caaacttaat cacaacgaga cgctaagtta cccgaatacc tacgatcaaa cgctgtatgc
     6241 cagcgtggag caggccttca acatgggcgc ggtagcggtt ggtgcgacta tctattttgg
     6301 ctcggaagag tcacgtcgcc agattgaaga aatttctgcg gcctttgaac gcgcgcacga
     6361 gctgggcatg gtgacagtgc tgtgggccta tttgcgaaac tccgccttta agaaagatgg
     6421 cgttgattac catgtttccg ccgacctgac tggtcaggcg aaccatctgg cggcaaccat
     6481 aggtgcagat atcgtcaaac aaaaaatggc ggaaaataac ggcggctata aagcgattaa
     6541 ttacggttat accgacgatc gcgtgtacag caagttaacc agcgaaaacc cgattgatct
     6601 ggtgcgttat cagttagcta actgctatat gggccgggct gggttgataa actccggcgg
     6661 tgctgcaggc ggtgaaactg acctcagcga tgcagtgcgt actgcggtta tcaacaaacg
     6721 cgcaggcgga atggggctga ttcttggacg taaggcgttc aagaaatcga tggctgacgg
     6781 cgtgaaactg attaatgccg tgcaggacgt ttatctcgat agcaaaatta ctattgcctg
     6841 atacgcctca tacgaacagc accaccccgt aggacggata agacgtttac actgcatccg
     6901 gcaacctatg cctgatgcga cgcggaagcg tcttatcatg cctacatagc actgccacgt
     6961 atgtttacac cgcatccggc ataaaaacac gcgcactttg ctacggcttc cctatcggga
     7021 ggccgttttt ttgcctttca ctcctcgaac aattttcata ttgttgtttt tgcgatcatt
     7081 atctcgacat ttaaaagcaa ataatttcat tatattttga aatcgaaagc aaacgacaag
     7141 atatgaaaat gtacgtggta tcgacaaagc agatgctgaa caacgcacag cgcggcggtt
     7201 atgcggttcc ggcattcaat attcacaatc tcgaaacgat gcaagtggtg gtggaaaccg
     7261 ctgccaacct gcatgcgccg gtcatcatcg ccggaacgcc tggcacattt actcatgctg
     7321 gtacagaaaa tctgttggcg ctggtcagcg cgatggcgaa gcaatatcac catccactgg
     7381 caattcatct cgaccatcac acgaaatttg acgatatcgc tcagaaggtt cgttctggcg
     7441 tgcgctcagt catgattgac gcctcgcatt tgccttttgc gcaaaatatt tcacgggtca
     7501 aagaggtggt ggatttttgc catcgctttg atgtcagcgt cgaggcggag ctggggcaac
     7561 ttggcggcca ggaagatgat gtgcaagtca atgaagtcga tgcgttgtac accaaccccg
     7621 cacaggcgcg tgaatttgcc gaggcaaccg ggattgattc cctggcggtc gccatcggca
     7681 cggctcacgg tatgtatgcc agcgcaccgg tgcttgattt ttctcgactg gagaacattc
     7741 gccagtgggt gaacttaccg ctggtgctgc atggcgcgtc aggattatcg actaaggata
     7801 ttcagcaaac catcaaactg gggatatgca aaatcaacgt tgcaacggag ctgaaaaatg
     7861 ccttcttgca gtcgttaaaa aattacctga ccgagcaccc cgaagcgacc gatccccggg
     7921 attatttgca gtcggctaaa tccgcaatgc gcgatgtggt gagcaaagtg attgccgatt
     7981 gtggctgcga gggcagggca taacgcacct gccatttaac aaggaaaaaa catgaaaacg
     8041 ttaattgccc ggcataaagc tggtgaacat atcggcatat gttcagtctg ttctgcccat
     8101 ccgttggtta tcgaagcggc gctggcattt gatcgcaaca gcacgcgcaa agtgctgatt
     8161 gaagcaacgt caaaccaggt caatcaattt ggcggttata ccggaatgac accggcagac
     8221 tttcgcgaat ttgtttttac gattgccgat aaagttgggt ttgcacgcga acgcattatt
     8281 ctcggcggcg atcatctggg accaaactgc tggcagcaag aaaatgcgga tgcggcgatg
     8341 gaaaaatccg tcgagctggt aaaagcatat gttcgtgctg gcttcagtaa aattcatctt
     8401 gatgcgtcaa tgtcctgcgc gggggatacc ataccgttag caccagaaac ggttgcggaa
     8461 cgagctgctg tgctttgctt tgctgcggaa agtgtggcga cagattgcca gcgtgagcaa
     8521 ctgagctatg tcattggcac tgaagttccg gttccgggcg gtgaggccag cgccattcag
     8581 tcagtacaca tcacccatgt tgaagatgcc gccaatactt tacgtacgca tcaaaaggcc
     8641 tttattgccc gtgggctgac agaggcgtta acacgtgtga ttgccatcgt ggtgcagccg
     8701 ggtgtggaat ttgatcacag caatattatc cattatcagc cgcaggaagc gcaggcgctg
     8761 gcgcaatgga tagaaaacac ccgaatggtt tatgaagcac attctaccga ttaccagacc
     8821 cggacggctt attgggaatt agtccgcgat cactttgcaa tattgaaagt cggtcccgca
     8881 ttaacctttg ctttacgcga ggcgatattt gcactggcac aaattgagca ggaacttatc
     8941 gcccccgaaa atcgcagcgg ttgcctggcg gtaattgaag aagtgatgct cgacgaaccg
     9001 caatactgga aaaaatatta tcgcacgggt tttaacgatt cattactgga tattcgttac
     9061 agcctgtcgg atcgtattcg ttattactgg ccgcatagtc ggattaaaaa tagcgttgaa
     9121 acgatgatgg tgaatctgca aggcgtggac atcccactgg gcatgattag tcagtatctt
     9181 cccaaacaat ttgaacgcat tcagtccggg gaattatcag caatgccgca tcagcttatt
     9241 atgaataaaa tttatgatgt tttgcgcgcc tatcgctacg gctgtgcgga ataaggacgg
     9301 tatatgacta acctgtttgt tcgtagcgga atttcttttg tcgatcgtag cgaagtttta
     9361 acctatatcg gtaatgagat gctcgcgaaa ggtgtggttc atgatacctg gccacaggca
     9421 ttaattgcca gagaagcaga attccctacc gggataatgc ttgagcagca cgccattgca
     9481 ataccgcatt gtgaggcgat tcatgctaag tcgtcagcca tttatctgtt aaggccaaca
     9541 aataaagttc attttcagca agcggatgat gataacgacg tggcggtatc gttggttatt
     9601 gcgttgattg tggaaaatcc gcagcagcaa ttgaaacttt tacgctgttt atttggcaag
     9661 ttacaacagc ccgatatcgt cgagacacta attactcttc ctgaaactca gttaaaggaa
     9721 tacttcacaa agtatgtttt agattcagac gaataaatcc ctctgtaaca ataaaaagga
     9781 ctatttatga aacgcaagat tattgtcgct tgcggaggcg cggttgcgac ctctacgatg
     9841 gcggcggaag aaattaaaga gttgtgtcag agtcataata ttcctgttga attaatccag
     9901 tgtcgggtta atgaaataga aacctatatg gatggtgtgc atttgatatg taccactgcc
     9961 agggtggatc gtagttttgg cgatattccg ttagttcacg gcatgccttt tgtttctggt
    10021 gtcggtatcg aagcattaca aaataaaatt ctgactatct tacagggatg acctatgttt
    10081 tcagaagtca tgcgttatat tctcgacctc ggccctacag tgatgctgcc ggttgtcatc
    10141 attatttttt ctaaaatatt aggcatgaag gcaggcgatt gctttaaagc gggtctgcat
    10201 atcgggattg gctttgttgg cattggcctt gtgattggct taatgctgga ttccattggt
    10261 ccggcggcga aagcgatggc ggaaaatttc gacctgaatc tgcatgtggt cgatgtcggc
    10321 tggccgggct cttcaccaat gacctgggcg tcgcaaattg cgctggtggc gattccgatt
    10381 gcgatattgg ttaacgtggc gatgttactg acccgtatga cgcgggtggt aaatgttgat
    10441 atctggaata tctggcatat gaccttcacc ggcgcgttgc tgcacctggc aaccggttca
    10501 tggatgatag ggatggcggg tgtggtaatt cacgcggcgt ttgtttataa gctcggcgac
    10561 tggtttgccc gcgatacccg aaatttcttt gagctggaag gtattgctat tccgcacggt
    10621 acgtcggcgt atatggggcc gattgcggtg ctggtcgatg ctatcatcga gaaaatccca
    10681 ggcgttaacc gaattaaatt tagcgccgac gatattcagc gcaaatttgg cccttttggc
    10741 gaacctgtca ccgtgggttt tgtgatgggg ctgattatcg gcatcctcgc gggttacgat
    10801 gtcaaaggtg tattgcagct ggcggtaaaa acggcggcag ttatgctgct gatgccacgg
    10861 atgattaaac ccatcatgga tggtttaacg cccatcgcta agcaggcccg tagtcgttta
    10921 caggcgaagt tcggcggtca ggagttcctg attggcctgg atcccgcatt actgctgggg
    10981 catacggcgg tggtatcggc aagcctgatt tttattccgc tcaccatttt aattgctgtt
    11041 tgtgttccgg gtaatcaggt actgccgttt ggcgatcttg ccactatcgg cttcttcgtg
    11101 gcgatggcgg tcgcggtgca tcgtggaaat ctgttccgca ccttaatctc gggtgtcatc
    11161 attatgagca tcaccctgtg gatcgcgacg caaactattg gtttgcacac ccaaccggcg
    11221 gctaatgctg gggcgttaaa agccgggggt atggtggctt caatggatca gggcggttct
    11281 cccattacct ggttactgat tcaggttttc tccccgcaaa atattcccgg tttcattatt
    11341 atcggcgcaa tttatctgac cggtattttc atgacctggc gtagagcgcg tggttttact
    11401 aaacaagaga aagccgttct cgcagaataa tttttacctg agggggaggt tatccccctt
    11461 aacaa
//
LOCUS       DAZPAB010000120        11417 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZPAB010000120 DAZPAB010000000
VERSION     DAZPAB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.120"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            327..1967
                     /gene="trg"
                     /locus_tag="ACHUPG_002796"
     CDS             327..1967
                     /gene="trg"
                     /locus_tag="ACHUPG_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR0862339.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2005..2928)
                     /locus_tag="ACHUPG_002797"
     CDS             complement(2005..2928)
                     /locus_tag="ACHUPG_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0862340.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3145..4488
                     /locus_tag="ACHUPG_002798"
     CDS             3145..4488
                     /locus_tag="ACHUPG_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0862341.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4713..6368
                     /gene="opgD"
                     /locus_tag="ACHUPG_002799"
                     /gene_synonym="mdoD"
     CDS             4713..6368
                     /gene="opgD"
                     /locus_tag="ACHUPG_002799"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR0862342.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6508..6732
                     /locus_tag="ACHUPG_002800"
     CDS             6508..6732
                     /locus_tag="ACHUPG_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR0862343.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6795..7331
                     /gene="rimL"
                     /locus_tag="ACHUPG_002801"
     CDS             6795..7331
                     /gene="rimL"
                     /locus_tag="ACHUPG_002801"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR0862344.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7326..8306)
                     /gene="ydcK"
                     /locus_tag="ACHUPG_002802"
     CDS             complement(7326..8306)
                     /gene="ydcK"
                     /locus_tag="ACHUPG_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR0862345.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8430..9422
                     /gene="tehA"
                     /locus_tag="ACHUPG_002803"
     CDS             8430..9422
                     /gene="tehA"
                     /locus_tag="ACHUPG_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR0862346.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9419..10012
                     /gene="tehB"
                     /locus_tag="ACHUPG_002804"
     CDS             9419..10012
                     /gene="tehB"
                     /locus_tag="ACHUPG_002804"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR0862347.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLTRKK"
     gene            10315..10971
                     /locus_tag="ACHUPG_002805"
     CDS             10315..10971
                     /locus_tag="ACHUPG_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFR0862348.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(10989..11417)
                     /gene="tnpA"
                     /locus_tag="ACHUPG_002806"
                     /pseudo
     CDS             complement(10989..11417)
                     /gene="tnpA"
                     /locus_tag="ACHUPG_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         2950 a         2667 c         2867 g         2933 t
ORIGIN      
        1 tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa
       61 agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca
      121 cgaaattgcg tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc
      181 gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga
      241 cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga
      301 gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt
      361 tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat
      421 tcgcattaag ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg
      481 atgttactgc ggagattgag attcggaccg ggttagcgaa cagttcagat tttttgcgtt
      541 cagcccggat caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa
      601 tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg
      661 cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc cttcgacact gaattaaatc
      721 aacgctttca ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca
      781 tgtttgaagc gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata
      841 ccgatatttt gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac
      901 tggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc
      961 tggtcatgac gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac
     1021 atgccgcaca acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg
     1081 cgggtcgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatgcag cattcactgg
     1141 ggatgacagt agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa
     1201 tttcagctgg caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac
     1261 aaactgccgc cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc
     1321 atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg
     1381 tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga
     1441 tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg
     1501 ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac
     1561 ggacactcgc aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat
     1621 cagtcaggtt aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca
     1681 ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg
     1741 atgaacaaag tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga
     1801 cgcaacagaa tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac
     1861 aggctgcacg attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg
     1921 cagaacctcg cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga
     1981 gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag cgttaatcat
     2041 caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc
     2101 tgcaccatgt cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac
     2161 agcgccagaa gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga
     2221 agtagacagc gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc
     2281 atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac
     2341 ccggcttagc gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa
     2401 cgattcaaga aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg
     2461 accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat
     2521 tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg
     2581 taatatcccc agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagacc
     2641 ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg
     2701 cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa
     2761 gcgagcgccc gtcagctgct ccagttcatt caatgtatta gagagcgcag gttgactcaa
     2821 attaagggtt tcagctgcgc gccccaaagt tccttgttgt gcgacagcta cgaatgtatg
     2881 aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa
     2941 aaacgaagcg gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgcaaaat
     3001 attattaaca attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg
     3061 cgagatcaca gtttgtaaat tcttcccgcc agagtgaatg cggttaccta cactccagat
     3121 tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa
     3181 cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg
     3241 ctggaactgg tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa
     3301 aaaatggtaa atgcagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc
     3361 gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac
     3421 ccggtgagct attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg
     3481 cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacatc cttactccgc
     3541 cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc
     3601 gatatcttca ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt
     3661 aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcaccag
     3721 ttagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct
     3781 gatgtggtct gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt
     3841 gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg
     3901 ccgccgcgcc gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag
     3961 acggtgttgt ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag
     4021 cagcgtggcg tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac
     4081 gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact
     4141 tttcctgaca gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg
     4201 acgccttcgg gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta
     4261 attgaacgtg gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc
     4321 gcggcgggga tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat
     4381 gccagtcgcg aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt
     4441 taccaggaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc
     4501 cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa
     4561 aaaaatgccg atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt
     4621 ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc
     4681 attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt
     4741 caatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcag
     4801 cattcgcggc agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc
     4861 tacagtcaat ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac
     4921 ctgacacgct ggcgacaatg acgccgcagg cttataacag tattcaatac gacgccgaaa
     4981 aatcgctctg gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa
     5041 tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg
     5101 aaattcactt tcgcccggag ttgttcaaat acaacgatgc aggtgttgat accaaacaat
     5161 tagaagggca aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg
     5221 cgcgccgtga tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat
     5281 atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag
     5341 agttccccga ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta
     5401 ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt
     5461 gtgagaaaag tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta
     5521 aacagctggg catttcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga
     5581 tgtgcgatac aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca
     5641 acggcgagtg gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca
     5701 ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc
     5761 aggacattat gggctggtat aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt
     5821 ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca
     5881 ttgtctgctt ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt
     5941 atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga
     6001 cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa
     6061 aatgggcgcg tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa
     6121 aaggcattga gccggtgatt acgctttcca gtggggaagc gaagcaaatc gaaattctct
     6181 atattgaacc catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca
     6241 ctgatccggt cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat
     6301 ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga
     6361 tgagttaatc gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat
     6421 cccgctcgca tttttcccta agttaaatga gtaatctgat ggtgtgtatt tcagatacac
     6481 cgtgtcagcc actaacaggg agtgcgtatg tttccagaat accgagattt aatatcccgt
     6541 ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat
     6601 gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg
     6661 aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc
     6721 aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat
     6781 tgactggaga taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg
     6841 ttgcggaaag tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac
     6901 agcagtcgct aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc
     6961 agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag
     7021 atgaacttat cggcgttatc tcgtttaatc gtattgaacc actgaataaa actgctgaaa
     7081 tcggctactg gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcaga
     7141 cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg
     7201 tggacaatcc gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc
     7261 tgaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta
     7321 tcgattcata ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact
     7381 tttggaccac ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt
     7441 aactccacat ggttttcaat tatcaccgca cctgtaatac ggctctcacc ttgtattaca
     7501 acgtgctcgt cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca
     7561 tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa
     7621 tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact
     7681 ttagcgcaat cgcatagcca gacgttgttc tcttcattac cttcaacact ggcaaaatca
     7741 aaaacttcgg cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt
     7801 gcctggtgga caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt
     7861 tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg
     7921 tgaccaaata ttctacattg cacgtaaact aatgagtcat gaatggtgac actgtcactt
     7981 atatatgcac cctggctgat ttcactatta tcgatccaga cgttatccgt tgcgtaaacc
     8041 tctccccaca gcacgctggt gccggtgata cgcgtattac cggaaatcac cgttccgccg
     8101 aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg
     8161 cgatcgatcc agccgccagc ggttcctgct atcacatcgt taaaatcgct aatggcgata
     8221 atctgccgta ataacacact ttttttagtg ccatcttcct gataactaaa ggctcgctgt
     8281 tcttcactta ggcgatattt gcgcatagcg ttttcccaca ggtgacttac tataaccgta
     8341 gcaaattctg cggctctggc tatgctcgag aaaattccat aaaatgcatt tcaaatatac
     8401 tttataaatt aaacaaaatg agtaagaaga tgcagagcga taaagtgctc aatttgccgg
     8461 caggctactt tggtattgtg ttggggacga tagggatggg atttgcctgg cgctatgcca
     8521 gccaggtttg gcaggtcagc cactggttag gggatgggct ggtgattctg gcgatgatca
     8581 tctggggatt attgactagc gcatttatta cccgactcat acgctttccg catagcgtgc
     8641 tggcggaagt tcgccatcca gtgctgagca gttttgtgag tttgtttccg gcaacgacga
     8701 tgctggtggc gattggtttt gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt
     8761 ttggtgtcgt ggttcagttg gcttatgccg cctggcaaac tgcgggatta tggcgcggat
     8821 ctcaccctga agaagctacc acgcctggac tgtatctgcc gacagttgcc aacaacttta
     8881 tcagcgcaat ggcctgtggt gcgttgggct acaccgacgc cggtctggtg tttttaggcg
     8941 caggcgtttt ctcatggcta agccttgaac cggtgatctt gcagcgtctg cgcagttcgg
     9001 gagaattacc cacggcactg cggacatcac tcggcattca gctcgctcct gcgctggtgg
     9061 cctgtagtgc ctggctgagc gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt
     9121 tcggttatgg actgctgcaa ctgctgttta tgctacgtct gatgccatgg tatctctccc
     9181 agccatttaa tgcttcattc tggagtttct cgttcggcgt atctgcactg gcaaccaccg
     9241 gtttgcatct ggggagtggc agcgataatg gatttttcca tacgctggcg gtgccgctgt
     9301 ttatctttac caattttatt attgcaatac tgctcatccg tacttttgcg cttctgatgc
     9361 agggaaaatt gttagtcaga accgagcgcg ccgttttaat gaaagcagag gacaaagaat
     9421 gatcattcgt gacgaaaact attttactga taaatatgaa ttaacccgca cacactctga
     9481 agtactggaa gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa
     9541 tggtcgtaac agtctttacc tggcagccaa tggttatgat gttgacgcat gggataaaaa
     9601 tgccatgagt atcgccaacg tcgagcgcat taaatccatt gaaaatctgg ataatttaca
     9661 cacccgggtc gttgatctga ataacctcac atttgatgga cagtacgatt ttattctttc
     9721 gactgtggtg ctgatgttcc ttgaggctaa aaccatccca gggttgattg ccaatatgca
     9781 acgttgcact aaacctggtg gttacaacct gattgtggcg gcgatggata ccgctgatta
     9841 tccatgtacc gtcggcttca cgtttgcctt caaagaggga gaattacgtc gatattacga
     9901 aggctgggag agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa
     9961 cggtaatcgt attaaactgc gtttcgccac gatgctgaca cgtaaaaaat gacccggtaa
    10021 gcacaaaacg cgtgaaaatt ccccacgctg agatgattta ctgttcttct tttcggtaag
    10081 catatttttt atcgaaggga tgtgaaatta atcacagtag tcgaagtttt ttagcagctt
    10141 aacttactga aatttaacta ctgatgattg acttagcccc tttttcggca ttgactatgt
    10201 cgtctgaaaa ggggctgaaa aatttatttt caccaacact ttttttgcca caacacgaag
    10261 cggcgctttt tgctataact tagaaagtaa tataatcatc tcaggaaact attcatgcgt
    10321 accacatcat ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc
    10381 tctaaaatca cccagccaga taaatataac aattactctg atttaaaaga aacaacctca
    10441 gcaacaggta aacctgtttt acgttgggta gacccgagtt ttgatcaaag caaatatgac
    10501 agcatcgtct ggaacccaat cacttattat ccggtaccaa aaccttctac ccaggtaggg
    10561 cagaaagttc tggataaaat tttgaactat accaacaccg aaatgaaaga agcgatagcg
    10621 cagcgtaaac cagtggttac caccgctggg ccgcgtagtc tgattttccg tggggccatt
    10681 accggtgtag ataccagcaa agaagggctg caattctatg aagtggttcc tgttgcatta
    10741 gtggttgcgg ggacgcaaat ggctacaggc caccgtacca tggatactcg cctctatttt
    10801 gaaggtgagc tgattgatgc agcgactaat aaaccggtta tcaaagtcgt tcgtcagggc
    10861 gaaggtaaag acctgaataa cgaaagtacg ccaatggctt tcgaaaatat taaacaagtt
    10921 attgatgaca tggcgaccga tgccaccatg tttgacgtta acaaaaagta gttccagacg
    10981 cgccatcgtt agatggcgct ttttatccgg tgcgccgtaa aaccccatcc ttcagggcgg
    11041 ggatataagg cgcggttttc cacctgacca ggtgtttgct gttgttcaat gtattggcgg
    11101 atgacggata ttggcgcacc tccgcagcta ctggcaaaat agccaggact ccacaaaacg
    11161 cctttgtagt aatacctgac tgcaatatct ggtcgatctc gtcgcagtaa cctacccgat
    11221 acgcctttga ggctgtttac cagactggat atcgccagtt tgggaggtta gttaattaac
    11281 aaatggacgt gatctggttc gccatccatt tcaaccagtt cagcttcaaa ataagcacat
    11341 acatttgaaa agtaagtgcg tagtttttct gtcgcgtcat ggtcaaaaat ctggcgtcgg
    11401 tatctggtga caaagac
//
LOCUS       DAZPAB010000121        11234 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZPAB010000121 DAZPAB010000000
VERSION     DAZPAB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11234
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.121"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..611)
                     /gene="hdhA"
                     /locus_tag="ACHUPG_002807"
     CDS             complement(<1..611)
                     /gene="hdhA"
                     /locus_tag="ACHUPG_002807"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR0862349.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
     gene            complement(723..1751)
                     /gene="malI"
                     /locus_tag="ACHUPG_002808"
     CDS             complement(723..1751)
                     /gene="malI"
                     /locus_tag="ACHUPG_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR0862350.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1926..3518
                     /gene="malX"
                     /locus_tag="ACHUPG_002809"
     CDS             1926..3518
                     /gene="malX"
                     /locus_tag="ACHUPG_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR0862351.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3528..4700
                     /gene="malY"
                     /locus_tag="ACHUPG_002810"
     CDS             3528..4700
                     /gene="malY"
                     /locus_tag="ACHUPG_002810"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR0862352.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4804..5805
                     /gene="add"
                     /locus_tag="ACHUPG_002811"
     CDS             4804..5805
                     /gene="add"
                     /locus_tag="ACHUPG_002811"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR0862353.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5841..6881)
                     /locus_tag="ACHUPG_002812"
     CDS             complement(5841..6881)
                     /locus_tag="ACHUPG_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR0862354.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7124..7249
                     /gene="blr"
                     /locus_tag="ACHUPG_002813"
     CDS             7124..7249
                     /gene="blr"
                     /locus_tag="ACHUPG_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR0862355.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7522..7737
                     /gene="ydgT"
                     /locus_tag="ACHUPG_002814"
     CDS             7522..7737
                     /gene="ydgT"
                     /locus_tag="ACHUPG_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR0862356.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7823..8263
                     /locus_tag="ACHUPG_002815"
     CDS             7823..8263
                     /locus_tag="ACHUPG_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR0862357.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8340..8921
                     /gene="rsxA"
                     /locus_tag="ACHUPG_002816"
     CDS             8340..8921
                     /gene="rsxA"
                     /locus_tag="ACHUPG_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR0862358.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8921..9499
                     /gene="rsxB"
                     /locus_tag="ACHUPG_002817"
     CDS             8921..9499
                     /gene="rsxB"
                     /locus_tag="ACHUPG_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR0862359.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9492..>11234
                     /gene="rsxC"
                     /locus_tag="ACHUPG_002818"
     CDS             9492..>11234
                     /gene="rsxC"
                     /locus_tag="ACHUPG_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR0862360.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPE"
BASE COUNT         2616 a         2891 c         2830 g         2897 t
ORIGIN      
        1 tctggtgtaa taacggattt cagggcatcg gttaatattg cccccggcgc aatgctattc
       61 acccgaatat ttttttcacc caggtcaaat gccatatttc tgaccagatg actggccgca
      121 gctttagatg atgcatagga agtcatgttt atatttttat tttctgccgc catagaagtg
      181 atggtcagaa taacgccacc tccatttttt tccatttctg gcgcaacaag ttgtgacaga
      241 tggaaaaaag aaaacacatt cagttcataa gcacggcgaa aatctgccat tggcatatca
      301 aacggtttag gaccaccgcc accggcgttg ttaaccagga tatcaacttt acccagatta
      361 ctgacggcaa agtctgccag tgcagagagt tcctgttcgg aagtaatatc acaacgccag
      421 gcaaatgcct gaccatccag ttgttgaatt tcgtctacaa catggttagc tgcgtcggcg
      481 ttaatatcac tgaccaccac agatgcgcca gctgtcgcga atgtaatggc gatttcttta
      541 ccaatacctg cacccgcacc tgtgatgatg gcgcattttc cgtcgagtct caggttgtca
      601 gaattaaaca cataaacctc ctgtgaactt cagtaaaaat aaaaggcacc gcctgtgtgc
      661 cacatttttt atcattaacc ttgaggaaag gacactacca agtatagcag ataaagagag
      721 gattatttcg ccgcaatgag ccgggcggga ataataagat tgcgtgaatg cgtctcttca
      781 tgggtgattt tttgcatcat gcgatccgca agtgtgatac caagttcccg cgctggcgtg
      841 ctggcccagg taacaggtat atcatcaagt gtggttggtg tcgcatcggt aaatgccgcc
      901 agcgaaacct gttgctcaaa gtaacgatcg actccgcttt ccccgctttg ccgccctgct
      961 ttcagcaaac caaaccatgc ccccatcgca atagtttcgt tatagcaaac cacggcactg
     1021 atggtcgggt tatgacgtaa aagcgccgtg atagcttccg cggcttgctt ctggctggaa
     1081 gtgcactcca acacccaatc gctgtgaaac ggcaggccaa attttagtag agttgcacaa
     1141 tagcccccca cccgttctgc acgggttaat gaggaacttt gccctcccag ccaggcgatc
     1201 cgctgatgcc cattgcgaat gagatgctcc gtcaacaact gtgcagcctg catgttgtcc
     1261 gggcgaaccg tatcaacatc atcaagataa ctggcacggg aagcgaaaat caccgggata
     1321 gctttttctt ctgccatccg ccgcaggtca tcgctgcttc ctgcagcccc ggcaattacc
     1381 acaccatcga caccctgatt cagtaacagt gaaaaccgct gtgccagctg ctcgccgtct
     1441 ttaccgccgt gaagcaaaaa aaccatccgt ccctgtgctt ccagagcttc cgtcaatccg
     1501 gccgtcaatt cggcgtaaaa cggcgcagat aaatcacgga cgatcaaacc aatgacgccg
     1561 ctttgcccgc cgcgcagcgc cgacgcctgg cgattgcgca caaatcccag ctcttcaatg
     1621 gcggcgttaa cgcgttctcc tgtggcggta gagattcgcc ctttgccatt aagcaccagc
     1681 gaaacggtac ttaccgacac gcccgcagcc agcgcaacat catgaatggt tatttttttg
     1741 gcggtagcca tgcagatgac ctactccctg attatgtgac agataaaacg ttttaccttt
     1801 tatttatctt atacccgcta ttatcgttgc gtaatgtgat ttatgactca ctaaaatttg
     1861 ataaaacgtt ttatcttctc gcgcaattta ctgaatccag attgttctct acgaggagtc
     1921 gttttatgac ggcgaaaaca gcaccgaaag tcacgctgtg ggagttcttc cagcagttag
     1981 gcaaaacctt catgttaccc gtggcattat tgtcgttctg cggcattatg ctcggcattg
     2041 gtagttctct tagcagccat gatgtcataa ccctgatccc ggtcctgggc aaccccgtgt
     2101 tgcaggctat ctttacctgg atgagtaaga ttggctcgtt tgcttttagt ttcctgcctg
     2161 tcatgttctg tatcgccatc ccgctgggcc tggcacgcga aaataaaggc gtagcggcat
     2221 tcgctggctt cgtcggttat gcggtaatga acctcgcggt aaacttctgg ttgaccaata
     2281 aaggcattct gccaaccaca gatgccgccg ttctgaaagc caataacatc cagagcattc
     2341 ttgggatcca gtcgatcgat accgggatcc tcggtgcggt gatcgccggt attatcgtct
     2401 ggatgctgca tgagcgtttc cataatatcc gcctgccgga tgcgctggca ttcttcggcg
     2461 gtacgcgctt cgtaccaatt atctcctcgc tggtgatggg ccttgtcggc ctggtgattc
     2521 cattagtctg gccgattttc gccatgggta ttagcggctt gggccatatg ataaacagcg
     2581 cgggtgattt cggaccgatg ctgtttggta ccggtgaacg tctgctgttg ccgtttggtc
     2641 tgcatcacat tctggtggca ttaattcgct ttaccgacgc aggcggcacg caggaggtct
     2701 gcggtcaaac cgtcagcggc gcactgacca tcttccaggc gcaattgagt tgcccgacca
     2761 ctcacggttt ttctgaaagc gtcacgcgtt tcctttcgca aggtaaaatg cctgcgtttc
     2821 tcggcggtct accaggtgca gcgttagcta tgtatcactg cgcgcgcccg gaaaatcgcc
     2881 ataaaattaa aggtctgctg atttctggcc tgatcgcctg cgtcgttggc ggcactaccg
     2941 aaccgctgga attcctgttc ctgttcgtag cgccagttct gtatgtcatc cacgcgctgt
     3001 taaccggcct cggcttcacc gtcatgtctg tgctcggcgt caccatcggt aataccgacg
     3061 gcaatatcat cgacttcgtg gtgttcggta ttttgcatgg tctgtcaacc aagtggtaca
     3121 tggtgccagt ggtggcggca atctggtttg tcgtttacta cgtcatcttc cgtttcgcta
     3181 tcacccgctt caatctgaaa accccggggc gcgatagcga agttgccagc tcaatcgaaa
     3241 aagccgttgc cggtgcgccg ggtaaatcag gttacaacgt tcctgcaatc ctcgaagcat
     3301 taggcggtgc cgacaatatt gtcagcctcg ataactgcat tacccgtctg cgtttgtctg
     3361 tggaagatat gtcgcttgtt aatgtgcagg cactgaagga caatcgggca attggcgtag
     3421 tacaacttaa tcaacataac ctgcaggttg ttatcgggcc acaagttcag tcagtaaaag
     3481 atgaaatggc cggtctgatg catactgtcc aggcataagg ataagatatg ttcgattttt
     3541 caaaggtcgt ggatcgtcat ggcacatggt gtacacagtg ggattatgtc gctgaccgtt
     3601 tcggcactgc tgacctgtta ccgttcacga tttcagacat ggattttgcc actgccccct
     3661 gcattatcga ggcgctgaat cagcgcctga tgcacggcgt atttggctac agccgctgga
     3721 aaaacgatga gtttctcgcg gctattgccc actggttttc cacccagcat tacaccgcaa
     3781 ttgatacgca gacggtggtg tatggccctt ctgtcatcta tatggtttca gaactgattc
     3841 gtcagtggtc tgaaacaggt gaaggcgtgg tgatccacac gcccgcctat gacgcatttt
     3901 acaaggccat tgaaggtaac cagcgcacag taatgcccgt tgctttagag aagcaggctg
     3961 acggctggtt ttgcgatatg ggcaagctgg aagccgtttt ggcgaaacca gaatgtaaaa
     4021 ttatgctcct gtgtaaccca cagaatccta ccgggaaagt gtggacgtgc gatgagctgg
     4081 agatcatggc tgacctgtgc gagcgtcatg atgtgcgggt tatttccgat gaaatccata
     4141 tggatatggt ctggggcgag cagccgcata ttccctggag taatgtggcg cggggagact
     4201 gggcgttgct gacatcggga tcgaaaagtt tcaatattcc cgcgctgacc ggtgcttacg
     4261 ggattataga aaatagcagc agccgtgatg cctatttatc ggcactgaaa ggacgtgatg
     4321 ggctttcttc cccttcggta ctggcgttaa ctgcccatat cgccgcctat cagcaaggtg
     4381 caccgtggct ggatgcctta cgcgtctatc tgaaagataa cctgacgtat atctcagata
     4441 aaatgaacgc cgcgtttcct gaactcaact ggcagatccc gcaatccact tatctggcct
     4501 ggcttgatct acgtccgttg aatattgacg acaacgcgtt gcaaaaagcg cttatcgagc
     4561 aagaaaaagt cgcgatcatg ccggggtata cctatggtga agaaggtcgt ggttttgtcc
     4621 gtctcaatgc cggctgccca cgttcgaaac tggaaaaagg tgtggctgga ttaataaacg
     4681 ccatccgcgc tgttcgctaa ccccaattgc gcaacgtaaa aaatcgttgc gcaatcgtgg
     4741 atttttaccc tgctttgttt ttataatggt gcgcactttt atatccagaa aaagagtgcg
     4801 accatgattg ataccaccct gccattaact gatatccatc gccaccttga tggcaacatt
     4861 cgtccccaga ccattcttga acttggccgc cagtataata tctcgcttcc tgcacaatcc
     4921 cttgaaacac tgattcccca cgttcaggtc attgccaacg aacccgatct ggtgagcttt
     4981 ctgaccaaac ttgactgggg cgttaaagtt ctcgcctctc ttgatgcctg ccgccgcgtg
     5041 gcatttgaaa acattgaaga tgcagcccgt aacggcctgc gctatgtcga gctgcgtttt
     5101 tcaccaggct acatggcaat ggcacataag ctgcctgtag cgggtgttgt cgaagcggtg
     5161 atcgatggcg tacgtgaagg ttgccgcacc tttggtgtgc aggcgaagct tatcggcatt
     5221 atgagccgaa ccttcggcga agccgcctgt cagcaagagc tggaggcctt tttagcccac
     5281 cgtgaccaga ttaccgcact tgatttagct ggtgatgaac ttggattccc gggaagtctg
     5341 ttcctttctc acttcaaccg cgcgcgtgat gcgggctggc atattaccgt ccatgcaggc
     5401 gaagctgccg ggccggaaag catctggcag gcgattcgtg aactgggtgc ggagcgtatt
     5461 ggacatggcg taaaagccat tgaagatcgg gcgctgatgg attttctcgc cgagcaacaa
     5521 attggtattg aatcctgtct gacctccaat attcagacca gcaccgtagc agagctggct
     5581 gcacatccgc tgaaaacgtt ccttgagcat ggcattcgtg ccagcattaa cactgacgat
     5641 cccggcgtac agggagtgga tatcattcac gaatataccg ttgccgcgcc agctgctggg
     5701 ttatcccgtg agcaaatccg ccaggcacag attaatggtc tggaaatggc tttcctcagc
     5761 gcagaggaaa aacgcgcact gcgagaaaaa gtcgccgcga agtaacaaaa atggatggtg
     5821 caaattgcac catccatttt tcacgcaagg cacaacgtcg cgcgatgttt ggcggattcg
     5881 atgcccagct caatcaactc cattacctgg attgcctggc ttgccggaac cggattttca
     5941 ccatcgccat ttaacgcatc acgaatcgcc gcatagtaag ccggataatt cccaggcacc
     6001 gtcaacagcg tttcttcgac acgttcctca ccttccacgc gggtaagtac gccatcacgc
     6061 atatcgtagc cccagtcttc ctgcggcaga cgctcgccat ttttcagacg ttcttcctgt
     6121 ggatcgaggc catatttcac ataactgcct cgggatccat gcacgatata ccgtgctgac
     6181 tcagcggctg ccagcatggt accgtgtaaa atgactcgcc gctgtggata ggacaagatg
     6241 gcgtggaaat aatcggtcga ctgcgctccg ggccgtaact gtgccaaatc taccgtcatg
     6301 ctgaccggta aaccaaatag cgtaatggcc tgatcaagaa gatgcggtgc taaatcgtac
     6361 cagataccgc tgcctgggcc gccctgttca cgccaacgat cgcgcacctg cggacggaag
     6421 cggtcaaaat gagactcaaa gtaagcaact tcacccagca cgccttccgc gagcaaacct
     6481 tttagcgtca agaaatcgct atcccaacgg cggttatgga atacagacag cacacgcccc
     6541 aggctttttg ccagcgcatc cagctctcgc gcttgtgaca gtgtcacggt aaagggttta
     6601 tcaacgacca catgtttacc cgcctcaagc gccgctttgg ctaacgggaa atgggtatcg
     6661 ttgggtgtag gaatgacaat caggtctatg ttgggatcgt taaacagatg cttcggctca
     6721 gagacaaccg taaccgttgg ccagtcggct tttacttttg tttcatcact gctggagatt
     6781 accgccagtt ccagcccggg cgtgcccgca atcaggggcg catggaaggt tttgctcgca
     6841 taaccatacc caatcaaccc aacacggatg ttgtcgctca tgtcattgcc tctcattgaa
     6901 gtatgatggc tatttgacac tatcctttac ccacgctcaa cagtttaata acctgccagc
     6961 aataagggat gttgtttaac ttaagtcaaa aaaatagcga attttccaac gacaaaagct
     7021 aaatatcgca aaaacctcag taaaaatctt gctggagcta ttattgctaa gtaacattta
     7081 ccccctgaag ttaatggatc aatcaagaga gatgtgggct gtaatgaatc gtcttattga
     7141 attaacaggt tggatcgttc ttgtcgtttc agtcattctt cttggcgtgg cgagtcacat
     7201 tgacaactat cagccacctg aacagagtgc ttcggtacaa cacaagtaag ctctgcactt
     7261 gtggagcgac atgctgcccg tccgggtgca tgttttcact tgtcggatat taaaccagga
     7321 atttattatc ttgttcgatg ttgttggtga ttgtcaggga tagtaaagcg cgttactctt
     7381 ttcagagcag tttttcatcc ctgaatattt gccatcagcg cattcttatt attgtaataa
     7441 cacaagcgtt cacttttggt gaaattttat catcactaat aatacctacc cctttggtat
     7501 taattatttt atggaccttt tatgactgtt caggactact tattaaaatt tcgcaaaatc
     7561 agttcactcg aaagtctgga aaaactctac gaccatctta attacaccct gacggacgat
     7621 caggaactga tcaatatgta tcgtgctgcc gatcaccgtc gcgcagagct ggtttctggc
     7681 gggcgtttgt ttgacctcgg ccaggtaccg aagtccgtct ggcactatgt ccaataaaga
     7741 aagtagcgat cggttataaa agccttataa taacgcccct aaaattagat ttgcctgttt
     7801 gcgtcgctat tctggagaac ctatgaccac cacaacgcca caacgtattg gaggctggtt
     7861 gcttggcccg ttggcctggc ttttagtcgc tctgttgagt acgacgctgg cgttgttgct
     7921 gtataccgct gcgttatctt ctccgcaaac atttcaaacg ctcggcggac aagcactgac
     7981 gacacaaatc ttatggggcg tttcttttat taccgctatc gccatgtggt attacacact
     8041 gtggttgacc atcgctttct ttaaacgtcg gcgttgtgtt cctaaacact atattatctg
     8101 gctgcttatt tccgtattgc tggcagtaaa agcattcgct ttttcacccg tggaagttgg
     8161 catagccgtt cgccagttgc tgtttacttt gttggcaacc gcattgatcg tcccctattt
     8221 caaacgctcg tcgcgggtaa aagcgacgtt tgtgaatccg taataacctt acagttaacc
     8281 tgttgtcgcc tgctctggat taacggataa taggcggctt ttttatttca ggccgaaaaa
     8341 tgactgacta cctgttactg tttgtcggaa ctgtactggt caataacttt gtactggtca
     8401 agtttctcgg tctctgtccg tttatggggg tttccaaaaa actggaaacc gcgatgggca
     8461 tggggctggc aacaacgttt gtgatgacgc tgacgtctat ttgcgcctgg cttatcgata
     8521 cgtggatttt gatcccactt aatctgattt acctgcgcac cctggcattt attctggtga
     8581 ttgctgtggt cgtgcagttc accgagatgg tggtgcgcaa aaccagcccg gtgctttacc
     8641 gtctgctggg gatttttttg ccgcttatca ccaccaactg tgcagtgctc ggcgtggcgt
     8701 tgctgaatat caatctcggg cacaatttct tgcagtcggc gctgtacggt ttttccgccg
     8761 ctgtcggttt ctcgctggtg atggtgctct tcgccgccat ccgcgaacgc cttgctgtgg
     8821 ctgatgtccc ggcacctttt cgcggtaatg ccattgcgtt aattaccgca ggtcttatgt
     8881 ctctggcctt tatgggcttt agtggtttgg tgaagttgta atgaatgcta tctggattgc
     8941 cgttgccgcc gtgagcctgc tgggcctggc gtttggcgcc attctgggtt atgcctcccg
     9001 ccgttttgcg gtggaagacg atccggtcgt tgagaaaatt gacgaaatct taccgcagag
     9061 ccagtgtggt cagtgcggtt atcccggctg tcgcccctac gcggaagcca tcagctgtaa
     9121 cggtgaaaaa atcaaccgtt gcgccccagg tggcgaagct gtgatgctaa aaattgccga
     9181 gttgcttaat gtcgagccgc agccgctgga tggcgaagcg caagagctaa cgcctgcgcg
     9241 gatggtggcg gttattgatg aaaataactg tattggctgc actaaatgta ttcaggcttg
     9301 tccggtagac gccatcgttg gcgctacccg tgtcatgcat acggtaatga gtgatctctg
     9361 tacgggctgc aatttatgtg ttgatccgtg cccgacgcac tgcatctcgt tgcaaccggt
     9421 cgcagaaaca cctgactcct ggaaatggga tctgaacacc attcccgtgc gtatcattcc
     9481 cgtggaacac catgcttaag ttattctctg cattcagaaa aaataaaatc tgggatttca
     9541 acggcggcat ccatccaccg gagatgaaaa cccagaccaa cggtaccccc ctgcgccagg
     9601 tactactggc gcagcatttt gttattccac tgaaacagca tattggcgct gaaggtgagt
     9661 tgtgcgttag cgtcggcgat aaagtattgc gcggccagcc gcttacccgt ggtcgcggca
     9721 aaatgctgcc tgttcacgcg cccacctcgg gtaccgttac ggctattgcg ccccactcta
     9781 cggctcatcc ttcagcttta gctgaattaa gcgtgattat tgatgccgat ggtgaagact
     9841 gctggatccc gcgcgacggc tgggtcgatt atcgctctcg cagtcgcgaa gagttaatcg
     9901 agcgcataca tcagtttggt gttgccgggc tgggcggtgc cggattcccg acaggcgtta
     9961 aattgcaggg tggtggagat aagattgaaa cgttgattat caacgctgct gagtgcgagc
    10021 cgtacattac cgccgatgac cgtttgatgc aggattgcgc ggctcaggtc gtagagggta
    10081 ttcgcattct tgcgcatatt ctgcagccac gcgaaattct tatcggcatt gaagataaca
    10141 aaccgcaggc gatttccatg ctgcgcgcgg tgctggcgga ctctaacgat atttctctgc
    10201 gggtgattcc aaccaaatat ccttctggcg gtgctaaaca attaacctac attctgaccg
    10261 ggaagcaggt tccacatggc gggcgttcat ccgatatcgg cgtattaatg caaaacgtcg
    10321 gcactgctta tgcagtgaaa cgtgccgtta ttgatggcga gccgattacc gagcgtgttg
    10381 taaccctgac tggcgaagca atcgctcgcc cgggcaacgt ctgggcacgg ctggggacgc
    10441 cagtgcgtca tttattgaat gatgccggat tctgcccctc tgccgatcaa atggtgatta
    10501 tgggtggccc gctaatgggc tttaccttgc catggctgga tgtcccggtc gtaaagatta
    10561 ccaactgtct gttggctccc tctgccaatg aacttggcga accacaggaa gaacaaagct
    10621 gcatccggtg tagcgcctgt gctgacgcct gccctgtgga tcttttgccg caacagttgt
    10681 actggttcag caaaggtcag caacacgata aagctaccac gcataacatt gccgactgca
    10741 ttgaatgtgg ggcttgcgcg tgggtttgcc cgagcaatat tcccttggtg caatatttcc
    10801 gtcaggaaaa agctgaaatt gcggctattc gtcaggaaga aaagcgcgcc gcagaagcca
    10861 aagcgcgttt cgaagcgcgc caggctcgtc tggagcgcga aaaagcgact cgccttgaac
    10921 gacataagag cgcagccgtt caacctgcag ccaaagataa agatgcgatt gctgccgctc
    10981 tggcgcgggt gaaagagaaa caggcccagg ctacacagcc tattgtgatt aaagcgggcg
    11041 aacgcccgga taacagtgca attattgcag aacgggaagc ccgtaaagcg caagccagag
    11101 cgaaacaggc agaactggag caaactaacg acgcagcaac cgttgctgaa ccacgtaaaa
    11161 ctgccgttga agcagctatc gcccgctcca aagcgcgcaa gctggaacag caacaggcta
    11221 atgcggaacc agaa
//
LOCUS       DAZPAB010000122        11192 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZPAB010000122 DAZPAB010000000
VERSION     DAZPAB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11192
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.122"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            169..1310
                     /gene="yghO"
                     /locus_tag="ACHUPG_002819"
                     /pseudo
     CDS             169..1310
                     /gene="yghO"
                     /locus_tag="ACHUPG_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
     gene            complement(1434..2204)
                     /gene="glcC"
                     /locus_tag="ACHUPG_002820"
     CDS             complement(1434..2204)
                     /gene="glcC"
                     /locus_tag="ACHUPG_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFR0862361.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            2455..3954
                     /gene="glcD"
                     /locus_tag="ACHUPG_002821"
                     /pseudo
     CDS             2455..3954
                     /gene="glcD"
                     /locus_tag="ACHUPG_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            3954..5006
                     /gene="glcE"
                     /locus_tag="ACHUPG_002822"
     CDS             3954..5006
                     /gene="glcE"
                     /locus_tag="ACHUPG_002822"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFR0862362.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            5017..6236
                     /gene="glcF"
                     /locus_tag="ACHUPG_002823"
                     /pseudo
     CDS             5017..6236
                     /gene="glcF"
                     /locus_tag="ACHUPG_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            6241..6645
                     /locus_tag="ACHUPG_002824"
     CDS             6241..6645
                     /locus_tag="ACHUPG_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFR0862363.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            6667..8838
                     /gene="glcB"
                     /locus_tag="ACHUPG_002825"
     CDS             6667..8838
                     /gene="glcB"
                     /locus_tag="ACHUPG_002825"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFR0862364.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFFRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9193..10779
                     /locus_tag="ACHUPG_002826"
     CDS             9193..10779
                     /locus_tag="ACHUPG_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HFR0862365.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
BASE COUNT         2631 a         2774 c         3100 g         2687 t
ORIGIN      
        1 aaattgacaa aaggcattga ggcgtttctg tgtagcctct gaccttatca taatcattaa
       61 ttaatactat ttattcgtcg tttgtattca ttgagttgat tattaagatg ccgctcgttg
      121 ctgttgcaac ctgtttttca ccattaatta tttgttatcc acagagatat ggaatgcgat
      181 ctgctgatga ttaaaattga aaaagtaatt aataaaaatg accttaaggc atttatcgct
      241 tttccgtcat cactttatcc cgacgatcca aactggatac cccctttatt cattgaacgc
      301 aacgagcatt tgtctgcgaa aaatccgggg acggaccata tcatctggca ggcgtgggtg
      361 gcaaaaaaag cggggcagat agttgggcgt attaccgcgc aaatagatac cttgcaccgt
      421 gagcgttacg gtgaagatac cggtcatttc ggcatgattg acgccattga tgatccgcag
      481 gattttgccg cgttgtttgg cgcagcggaa gcgtggttga agtcacaagg tgcaagtaag
      541 atcagcgggc ctttcagcct gaatatcaat caggaaagcg gattactgat tgaaggtttt
      601 gacacaccac cctgtgcgat gatgccacac ggcaaaccgt ggtatgccgc gcatattgaa
      661 caactgggtt atcacaaagg cattgattta ctggcgtggt ggatgcagcg aaccgatctc
      721 actttctctc cggcgctaaa aaaactgatg gatcaggtgc gcaaaaaggt gaccattcgc
      781 tgcatcaatc gtcagcggtt tgccgaagag atgcagatcc tgcgtgagat tttcaactct
      841 ggctggcagc acaactgggg atttgtgccg tttaccgaac atgaattcgc gaccatgggc
      901 gaccaactta aatatctggt gccggacgat atgatctaca tcgctgagat tgattctgca
      961 ccctgcgcgt ttattgtggc ttgcctaaca tcaacgaggc gattgccgat ctgaacggat
     1021 cgctctttcc cttcggctgg gcaaaattgc tgtggcgttt gaaagtcagc ggtgtgcgaa
     1081 ctgcgcgagt accgctgatg ggcgtgcgcg acgagtatca gttcagccgc atcggcccgg
     1141 tgattgcgct gttattgatt gaagccttac gcgatccgtt tgcccgccgg aagatcgatg
     1201 cgctggagat gtcatggatc ctcgaaacca atacaggcat gaataacatg ctggaacgca
     1261 ttggggcgga gccgtacaag cgttaccgat tgtatgaaaa gcaaatttaa taccagatcc
     1321 tgtttagcgc aggggcggtc attcgggaga gggtatagat gaacagattt aacaccagat
     1381 cctgttaagt gcaggggcgg tcccctcgcc ttggggatag ggtaaaactc tgctcacatt
     1441 cccaccttgt tcatctccag cggcaccgcc gagcgaatca aatggtggcc ttccagctcg
     1501 atatcgtgga gattcttttt cacggtccgt acatgatcgc gcgctgcgcg ctgagcgacg
     1561 tgcggcagcc gctgcaacac cgcgttgtag atccgcgcat gctggcgatc gatctgcttt
     1621 ttttgctgtg gtcgatggta gagattattt acgctggcaa acactgaatt aaacatcaga
     1681 tcggtcaatg attgcagcgt aaacaccagc acctgattgt gagaagcctg acaaatggcg
     1741 agatggaaag cgtgatccaa ctgcgcatgt tcgatcagcg aaatctcttt gtggttctca
     1801 ctggcggcga gcattttttc ataacagcgg gttatcacaa caaaatcagc ctgcgttccc
     1861 agcgttgccg ccagccttgc cgattcgccc tccagtaatg cgcgaacgtc gagcagatcg
     1921 tacagcgttc gcggctgcgt actgaacaga tggatcagcg ggctggtgtc ctgctcccga
     1981 ttaagccgtg cgacatgaga atcgcgaccc tgcgccgttt caataatccc gcgcccgcgc
     2041 agcacggtca gcccttcacg cagtgcggag cgtgagaagc cgagcttttc acacagtcga
     2101 cgttccgagg gaagcggctg accgaccttc agtacgccgt cgataattaa ccgttcgata
     2161 ctctctgcaa ccacttcgca aatagggcga cgttcatctt tcattcccgg acctcgtgca
     2221 caggtaggac caatttttct gttcttaact cgcaaaacac gcacatcacg caagtgatta
     2281 tgttacatca atttaacatt aagttaacca agacaaggtc acagagctgg aaaaaaaatg
     2341 gtctgaccgg tagctaaaga gatagacgaa aacgaaaagc ccgcttaata actgttcaca
     2401 gaagcagcgc gcaaaaatca gctgccacac aacacaacaa agcgaagcct actcatgagc
     2461 atcttgtacg aagagcgtct tgatggcgct ttacccgatg tcgaccgcac atcggtactg
     2521 atggcactgc gtgagcatgt ccctggactt gagatcctgc ataccgatga ggagatcatt
     2581 ccttacgagt gtgacgggtt gagcgcgtat cgcacgcgtc cattactggt tgttctgcct
     2641 aagcaaatgg aacaggtgac agcgattctg gctgtctgcc atcgcctgcg tgtaccggtg
     2701 gtgacccgtg gtgcaggcac cgggctttct ggtggcgcgc tgccgctgga aaaaggtgtg
     2761 ttgttggtga tggcgcgctt taaagagagc ctcgacatta accccgttgg tcgccgcgcg
     2821 cgcgtgcagc cgggcgtatg taacctggcg atctcctagg ccgttgcgcc gcataatctc
     2881 tattacgcgc cggatccttc ctcacaaatc gcctgttcaa ttggcggcaa tgtggcggaa
     2941 aatgccggtg gcgtccactg cctgaaatat ggtctgaccg tacataacct gctgaaaatt
     3001 gaagtgcaaa cgctggacgg cgaggcgctg acgctgggat cggacgcgct ggattcacct
     3061 ggttttgacc tgctggcgct gttcaccgga tcggaaggta tgctcggcgt gaccaccgaa
     3121 gtgacggtaa aactgctgcc gaagccgccc gtggcgcggg ttctgttagc cagctttgac
     3181 tcggtagaaa aagccggact tgcggttggt gacatcatcg ccaatggcat tatccccggc
     3241 gggctggaga tgatggataa cctgtcgatc cgcgcggcgg aagattttat tcatgccggt
     3301 tatcccgtcg acgccgaagc gattttgtta tgcgaactgg acggcgtgga gtctgacgta
     3361 caggaagact gcgagcgggt taactacatc ttgttgaaag cgggcgcgac tgacgtccgt
     3421 ttggcacagg acgaagcaga gcgcgtacgt ttctgggccg gtcgcaaaaa tgcgttcccg
     3481 gcggtaggac gtatctcccc ggattactac tgcatggatg gcaccatccc gcgtcgcgcc
     3541 ctgcctggcg tactggaagg cattgcccgt ttatcgcagc aatatgattt acgtgttgcc
     3601 aacgtctttc atgccggaga tggcaacatg cacccgttaa tccttttcga tgccaacgaa
     3661 cctggtgaat ttgcccgcgc ggaagagctg ggcgggaaga tcctcgaact ctgcgttgaa
     3721 gttggcggca gcatcagtgg ggaacatggc atcgggcgag aaaaaatcaa tcaaatgtgc
     3781 gcccagttca acagcgatga aatcacgacc ttccatgcgg tcaaggcggc gtttgacccc
     3841 gatggtttgc tgaaccctgg gaaaaacatt cccacgctac accgctgtgc tgaatttggt
     3901 gccatgcatg tgcatcacgg tcatttacct tttcctgaac tggagcgctt ttgatgctac
     3961 gcgagtgtga ttacagtcag gcgctgctgg agcaggtgaa tcaggcaatt agcgataaaa
     4021 cgccgctggt gattcagggc agcaatagca aagccttttt aggtcgccca gtcaccgggc
     4081 aaacgctgga tgtgcgttgt catcgcggca ttgttaatta cgacccgacc gagctggtga
     4141 taaccgctcg tgccggaacg ccgctggtgg cgattgaagc ggcgctggag agcgccgggc
     4201 aaatgcttcc ctgcgagccg ccgcattatg gtgaagaagc cacctggggc gggatggtcg
     4261 cctgcgggct ggcggggccg cgtcgcccgt ggagcggttc ggtccgcgat tttgtcctcg
     4321 gcacgcgcat cattaccggc actggaaaac atctgcgttt tggtggcgaa gtgatgaaaa
     4381 acgttgccgg atacgatctc tcacggttaa tggccggaag ctacggttgt cttggcgtgc
     4441 tcactgaaat ctcaatgaaa gtgttaccgc gaccgcgtgc ctctctgagc ctgcgtcggg
     4501 aaatcagcct gcaagaagcc atgagtgaaa tcgccgagtg gcaactccag ccattaccca
     4561 ttagtggctt atgttacttc gacaatgcgt tgtggatccg ccttgagggc ggcgaaggat
     4621 cggtaaaagc agcgcgtgaa ctgctgggtg gcgaagaggt tgtgggtcag ttctggcagc
     4681 aattgcgtga acaacaactg ccgttcttct cgttaccagg taccttatgg cgcatttcat
     4741 tacccagcga tgcgccgatg atggatttac ccggcgagca actgatcgac tggggcgggg
     4801 cgttacgctg gctgaaatcg acagccgatg acaatcaaat ccatcgcatc gcccgcaacg
     4861 ctggcggtca tgcgacccgc tttagtgccg gagatggtgg ctttgccccg ctatcggctc
     4921 ctttattccg ctatcaccag cagcttaaac agcagctcga cccttgcggc gtgtttaacc
     4981 ccggtcgcat gtacgcggaa ctttgaggag caggctatgc aaacccgatt aaccgaagag
     5041 atgcggcaga acgcgcgctg gaagccgaca gcatcctgtg cgcctgtgtt cactgcggat
     5101 tttgtaccgc aacctgccca acctatcagc ttctgggcga tgaactggac gggccgcgcg
     5161 ggcgcatcta tctgattaaa caggtgctgg aaggcaacga agtcacgctt aaaacacagg
     5221 agcatctcga tcgctgcctc acttgccgca attgtgaaac cacctgtcct tctggtgtgc
     5281 gctatcacaa tttgctggat atcgggcgtg atattgtcga gcagaaagtg aaacgcccac
     5341 tgccggagcg aatgctgcgc gaaggattgc gccaggtagt gccgcgtccg gcggtcttcc
     5401 gtgcgctgac gcaggtaggg ctggtgctgc gaccgttttt accggaacag gtcagagcaa
     5461 aactgcctgc tgaaacggtg aaagctaaac cgcgtccgcc gctgcgccat aagcgtcggg
     5521 ttttaatgct ggaaggctgc gcccagccta cgctttcgcc caacaccaac gcggcaactg
     5581 cgcgagtgct ggatcgtctg gggatcagcg tcatgtcagc taacgaagca ggctgttgtg
     5641 gcgcggtgga ctatcatctt aatgcgcagg agaaagggtt ggcacgggcg cgcaataata
     5701 ttgatgcctg gtggcccgcg attgaagcag gtgccgaggc aattttgcaa accgccagcg
     5761 gttgcggcgc gttcgtcaaa gagtatgggc agatgctgaa aaacgatgcg ttatatgccg
     5821 ataaagcgcg tcaggtcagt gaactggcgg tcgatttagt cgaacttctg cgcgaggaac
     5881 cgctggaaaa actggcaatt cgcggcgata aaaaactggc cttccactgc ccgtgtaccc
     5941 tacaacatgc gcaaaagctg aacggcgaag tggaaaaagt gttacttcgt cttggattta
     6001 ccttaacgga cgttcccgac agccatctgt gctgcggttc agcgggaaca tatgcgttaa
     6061 cgcatcccga tctggcacgc cagctgcggg ataacaaaat gaatgcgctg gaaagcggca
     6121 aaccggaaat gatcgtcacc gccaacattg gttgccagac gcatctggcg agcgccggtc
     6181 gtacctctgt gcgtcactgg attgaaattg tagaacaagc ccttgaaaag gaataaaaaa
     6241 atgaaaacta aagtcattct tagccagcaa atggcgagtg caattattgc cgcaggtcag
     6301 gaagaggcgc agaaaaataa ctggtctgtt tccattgctg ttgccgatga cggcggtcat
     6361 ctgctggcgt taagtcgcat ggacgattgc gcgccgattg cggcttacat ctcccaggag
     6421 aaagcgcgta ccgccgcgct ggggcgtcgt gaaactaagg gctatgaaga gatggtgaac
     6481 aacggacgta ccgcgttcgt gactgcgccg ttactgacgt cgctggaagg cggcgtaccg
     6541 gttgttgttg atgggcaaat tattggtgcc gtgggcgttt ctggtttaac cggagcacag
     6601 gatgcgcagg tcgcgaaagt ggcagcagcg gtgttggcga aataagcgaa aacgaggaga
     6661 gaaacaatga gtcaaaccat aacccagggc cgtttacgca ttgacgccaa ttttaaacgt
     6721 tttgtggatg gagaagtttt gccgggcgtg gaactggacg ctgctgcatt ctggcacaat
     6781 gttgatgaga tcgttcacga tctggcacca gaaaatcgtc agttgctggc agaacgcgat
     6841 cgcattcagg cggcgcttga tgagtggcat cgcagcaatc cggggccggt aaaagataaa
     6901 gcggcctata aatctttctt tcgtgaactg ggctacctgg tgccgcaacc ggagcgcgtg
     6961 acggtggaaa ccacgggcat tgacagcgaa atcaccagtc aggcggggcc acagctggtg
     7021 gttccggcaa tgaacgcccg ctacgcgctg aacgcggcga acgctcgctg gggctcactg
     7081 tacgatgcgt tatacggcag cgacatcatc ccgcaggaag gggcgatggt cagtggctac
     7141 gatccgcagc gcggtgagca ggttatcgcc tgggttcgac gtttcctcga tgaatcacta
     7201 ccgctggaaa acggcagcta tcaggatgtg gtggcgttta aggtggtcga taaacaatta
     7261 cgcatccagt tgaaaaatgg taaagaaacc acgttacgta ctccagcaca gtttgtcggt
     7321 taccgtggcg atgccgctgc gccgacctgc attttgctga aaaataacgg cctgcatatt
     7381 gaactgcaaa ttgatgccaa cgggcggatt ggcaaagacg atccggcgca catcaacgat
     7441 gttatcgtcg aagctgctat cagtaccatt ctcgactgcg aagattcggt cgcggcggtt
     7501 gatgcggaag ataaaatcct gctgtaccgc aatctgctgg gcctgatgca ggggactctg
     7561 caagagaaaa tggagaaaaa cggtcggcaa atcgtacgta aactgaatga cgatcgtcag
     7621 tacaccgccg ccgatggctc tgaaatttct ctgcacggac gctcgttgct gtttatccgc
     7681 aacgtgggtc atttgatgac cattcctgtg atttgggaca gcgaaggcaa tgaaatcccg
     7741 gaaggtattc ttgatggcgt catgactggc gcgattgccc tctatgattt aaaagtgcag
     7801 aaaaactcgc gcactggcag cgtctatatt gtgaaaccga aaatgcacgg tccgcaggaa
     7861 gtggcgttcg ccaacaaact gtttacccgc attgagacaa tgctcggtat ggcaccgaat
     7921 accctgaaaa tgggcattat ggatgaagaa cgccggacct cgctgaactt gcgtagctgt
     7981 atcgctcagg cgcgcaaccg cgtggcgttc atcaataccg gtttcctcga ccgtaccggc
     8041 gatgaaatgc attcggtgat ggaagctggc ccgatgctgc gtaaaaacca gatgaaatcg
     8101 acgccgtgga tcaaagccta cgaacgtaat aacgtgcttt ccggtctgtt ctgtgggctg
     8161 cgcggtaaag cgcaaatcgg taaaggcatg tgggcaatgc cggacctgat ggcagacatg
     8221 tacagccaga agggcgacca actgcgtgcc ggggcaaaca cagcctgggt tccgtcacca
     8281 accgctgcta cgctccatgc gctgcactac caccatacca acgtacagag cgtgcaagcc
     8341 aacattgccc agaccgagtt cagtgctgaa tttgaaccgc tgctggacga tctgctgact
     8401 attccggtgg ctgaaaacgc taactggtcg gcgcaagaga tccaacaaga gctggataac
     8461 aacgtgcagg ggattctggg gtacgtggtg cgttgggtgg agcaggggat tggttgttca
     8521 aaagtgccgg atattcacaa cgtggcgctg atggaagacc gcgcaacgct gcgtatctcc
     8581 agccagcata tcgccaactg gttacgtcac ggtattctga ccaaagaaca ggtgcaggcg
     8641 tcgctggaga atatggcgaa agtggttgat cagcaaaatg ctggcgatcc ggcttatcgt
     8701 ccgatggcgg ggaatttcgc taactcgtgt gcttttaaag ctgccagcga tttaatcttc
     8761 ctcggcgtga aacagccaaa cggttatacc gaaccgttat tacacgcctg gcgtttacgc
     8821 gaaaaagaaa gtcattaata gcgtcaaaaa agccccggca agttttattg tcggggctat
     8881 gatagtaact gcggtagtac atttatcccg gaaataaccg gagcgattat cagggtttaa
     8941 caatattaaa tacccgtagc gcattactag cgggcctaaa acaacaatat ggacatctta
     9001 acgatactct gcgcctgcgt gtgcggaaca gaaaccctta cccgatatac ttgttatgcc
     9061 agaggtagca attgcgttag cggcgtactg ttgctgtaaa gtataacgcc tatattttac
     9121 agttcagtcc tcttttgctt attacaacct taagtatatc aagcatataa agataataag
     9181 agactgaaca atatggttac ctggacccaa atgtatatgc cgatgggagg actggggcta
     9241 tccgctctgg tcgccctgat cccgataata ttcttcttcg ttgcactcgc ggtattacgt
     9301 ctgaaaggac atgttgctgg agcaataacc cttatattat ctatcctgat tgcgatattc
     9361 gcctttaaaa tgccgattga tatggcattt gctgctgcgg gctatggctt tatttatgga
     9421 ttatggccaa ttgcgtggat tattgtcgcg gcggtgttcc tgtataaatt aaccgttgcc
     9481 agcgggcagt tcgatattat ccgcagctcg gttatctcca tcaccgacga tcagcgtttg
     9541 caggtgttac tgattggttt ctcctttggt gcgttgctgg aaggagctgc tggctttggt
     9601 gcgacggtgg cgtttggtgc gttgggcgtg ccgattctgg tcgccggtca ggtaacggga
     9661 atcgatccgt tccacattgg cgcaatggcg ggacgtcagt taccgttcct gtcggttctt
     9721 gtgccgttct ggctggtagc aatgatggac ggctggaaag gggtgaaaga gacgtggcca
     9781 gcggcgctgg ttgctggggg aagcttcgct gtcactcagt tctttacctc taactatatt
     9841 ggtccggaac tgccggatat tacttcggcg ctggtgagta tcgtctcact cgctttattc
     9901 cttaaagtct ggcggccgaa aaataccgaa acggcaatca gcatggggca atccgcaggt
     9961 gcgatggtgg taaataagcc atcttctggc ggtcccgtgc cttcagaata tagtctgggg
    10021 caaatcattc gtgcgtggtc accgttttta atcttaacgg tgctggtcac catctggacc
    10081 atgaagccgt ttaaagcgtt atttgctccg ggcggcgcgt tttattcact ggtgattaat
    10141 ttccagatcc ctcatttgca tcaacaagtg ttgaaagcgg cacccattgt cgcccaacca
    10201 acgccaatgg atgcggtgtt taaattcgac cctctctcgg ctggcggcac cgctattttt
    10261 attgcggcga ttatctctat cttcatcctc ggcgtgggca tcaagaaagg gattggcgtc
    10321 tttgccgaaa tgctaattag tctgaagtgg ccgatattgt cgattggcat ggtgctggca
    10381 ttcgccttcg tcaccaacta ttccggcatg tccaccacgc tggcgctggt actggcaggt
    10441 acaggcgtga tgttcccgtt tttctcaccg tttctcggct ggctgggcgt attccttacc
    10501 gggtcggaca cttcttcaaa cgccctgttt ggttcactgc aatcgaccac ggcgcagcaa
    10561 atcaacgtct ctgacactct gctggtggca gcaaatacca gcggcggcgt aactggcaag
    10621 atgatctccc cgcaatctat cgccgtggcc tgcgccgcga cgggcatggt gggccgagaa
    10681 tctgaactgt tccgctacac cgtgaagcac agtctgattt ttgccagcgt tatcaccctg
    10741 ctgcaggcgt atgtgtttac cgggatgtta gtctcgtaaa tcaataaagc cggatgaagg
    10801 taaatatcac ccggctttcc atttgtctgt cgtttatctc gccagggaac cgtttttacg
    10861 attggcttct ttatacgtag tctccgtttt acgatgcgaa ttgtttaata tttatacaac
    10921 ttggcttctg tgctggttat aaagtaccca ctttcatgag atcaatttta gtatgttatt
    10981 acattgcttc atctgttgct ttcatgacga atggcgtttt ttctcttatt tatatttttc
    11041 actagaacaa tgagtagatc aaaattccca aaactcccaa aaacataaga ttattcgcaa
    11101 agctgatttt gcagaataat gagaactttt aagatttgtc gaggatcaat tttaagcaat
    11161 ttatctccac ttgttacata aaagatgccg tt
//
LOCUS       DAZPAB010000123        11170 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZPAB010000123 DAZPAB010000000
VERSION     DAZPAB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11170
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.123"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..112
                     /locus_tag="ACHUPG_002827"
     CDS             <1..112
                     /locus_tag="ACHUPG_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR0862366.1"
                     /translation="RFLLIFFTDYSHFMDRITLPSTDTASGKGKWKIITA"
     gene            91..195
                     /gene="speFL"
                     /locus_tag="ACHUPG_002828"
     CDS             91..195
                     /gene="speFL"
                     /locus_tag="ACHUPG_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR0862367.1"
                     /translation="MENNNRLMPHIRRTTHIMKFTHRNSFDFHFFNAR"
     gene            506..2703
                     /gene="speF"
                     /locus_tag="ACHUPG_002829"
                     /pseudo
     CDS             506..2703
                     /gene="speF"
                     /locus_tag="ACHUPG_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            2700..4019
                     /gene="potE"
                     /locus_tag="ACHUPG_002830"
     CDS             2700..4019
                     /gene="potE"
                     /locus_tag="ACHUPG_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR0862368.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4075..5715)
                     /gene="pgm"
                     /locus_tag="ACHUPG_002831"
     CDS             complement(4075..5715)
                     /gene="pgm"
                     /locus_tag="ACHUPG_002831"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR0862369.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5741..6286)
                     /gene="seqA"
                     /locus_tag="ACHUPG_002832"
     CDS             complement(5741..6286)
                     /gene="seqA"
                     /locus_tag="ACHUPG_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR0862370.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6471..7235
                     /gene="ybfF"
                     /locus_tag="ACHUPG_002833"
     CDS             6471..7235
                     /gene="ybfF"
                     /locus_tag="ACHUPG_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0862371.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7375..7668
                     /gene="ybfE"
                     /locus_tag="ACHUPG_002834"
     CDS             7375..7668
                     /gene="ybfE"
                     /locus_tag="ACHUPG_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR0862372.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7808..8338
                     /gene="fldA"
                     /locus_tag="ACHUPG_002835"
     CDS             7808..8338
                     /gene="fldA"
                     /locus_tag="ACHUPG_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR0862373.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8548..8634
                     /gene="uof"
                     /locus_tag="ACHUPG_002836"
     CDS             8548..8634
                     /gene="uof"
                     /locus_tag="ACHUPG_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR0862374.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8627..9073
                     /gene="fur"
                     /locus_tag="ACHUPG_002837"
     CDS             8627..9073
                     /gene="fur"
                     /locus_tag="ACHUPG_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR0862375.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9157..9483)
                     /gene="chiQ"
                     /locus_tag="ACHUPG_002838"
     CDS             complement(9157..9483)
                     /gene="chiQ"
                     /locus_tag="ACHUPG_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR0862376.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9520..10926)
                     /gene="chiP"
                     /locus_tag="ACHUPG_002839"
     CDS             complement(9520..10926)
                     /gene="chiP"
                     /locus_tag="ACHUPG_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFR0862377.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2715 a         2761 c         2787 g         2907 t
ORIGIN      
        1 tcgttttttg ttgatcttct tcacagatta tagccatttc atggatagaa taactctacc
       61 ttcaactgac acagcaagcg gtaaaggtaa atggaaaata ataaccgctt aatgcctcat
      121 ataaggcgga caacccatat catgaagttt acccatcgta atagttttga ctttcatttc
      181 tttaatgccc gctagtcttc tgactaaagg gcaccccaac gtacaggtct ccctgacttt
      241 aagcattaca ggttaatacc tgtattcctc ggtgctcata tactgctaac ccttttaaac
      301 tctaaataat tcgagtcgca gcacttgcaa cttgaggtat gacgagtata gccagttacc
      361 gggctggtct gggttattgc atctgcaaaa agcaaactac tgatttattt atcagcggtg
      421 gagctttgct ttttttccga cgtgatcgat ttctcctttg agaaattgag gacctgctat
      481 tacctgaaat aaagagatga ataaaatgtc agaattaaaa attgccgtta gccgttcttg
      541 cccggattgt ttttccactc atcgtgcatg cgtgaatatt gacgaaagta attatattga
      601 cgttgccgcc attattttat cagtcaatga tgttgaacgt ggaaaactcg ataaaataga
      661 cgctactggc tatggcattc ctgtttttat tgcaacggaa aatgaagaac gtgtaccagc
      721 agaatatctg ccacgtattt ctggtgtctt cgagcattgc gaatcacgga aagagtttta
      781 tggacgtcag ttagaaaccg ctgctagcca ttatgaaacg caactgcgcc cgcccttctt
      841 ccgcgcactg gtcgattatg tcaatcaggg caatagcgct tttgactgcc cgggtcatca
      901 gggaggtgag tttttccgtc gtcatccggc agggaatcag ttcgtggagt acttcggaga
      961 aacgctgttc cgttccgact tatgcaacgc agacgtagca atgggcgatc tgctgattca
     1021 cgaaggcgcg ccatgcattg cacagcaaca tgcggcaaaa gtgtttaatg ccgataaaac
     1081 atacttcgtt ctaaatggca cttcatcttc taacaaagtg gttttaaacg ccctgctaac
     1141 accgggtgat ctggtgctgt ttgatcgcaa taaccacaaa tctaaccacc acggagcgtt
     1201 gctacaggct ggtgcaacac cggtttatct ggaaacggca cgtaacccgt atggctttat
     1261 cggtggcatt gatgcgcact gttttgaaga aagttacctg cgtgagctga tcgcggaagt
     1321 cgcaccgcag cgggcaaaag aggctcgtcc tttccgcctc gctgtgattc agttaggcac
     1381 ctacgacggt acgatttata acgcccgcca ggtggtggat aaaattggtc atctgtgtga
     1441 ctacattctg tttgactccg catgggttgg ctatgagcaa tttatcccga tgatggctga
     1501 ctgttcgccg ctgttgctgg atcttaatga gaacgatccg ggtattctag ttacgcaatc
     1561 cgtacataaa cagcaggctg gtttttctca aacttcacaa atccataaaa aagacagcca
     1621 catcaaaggg caacagcgtt atgtaccgca caaacgcatg aacaacgcct ttatgatgca
     1681 cgcctccacc agcccgttct atccgctgtt tgccgcactg gatatcaacg ccaaaatgca
     1741 tgaaggtgtc agcggtcgta atatgtggat ggattgtgtg gtaaatggca ttaatgcccg
     1801 caaactgatc ctcgataact gtcagcatat tcgtccgttc gtacctgaac tggtggatgg
     1861 taaaccctgg cagtcgtatg aaacagcgca aattgcggtt gatctgcgct tcttccagtt
     1921 tgtaccaggg gaacactggc attcttttga aggctatgca gagaatcaat actttgtcga
     1981 tccttgcaaa ctgttgctga caaccccagg tattgatgca cgtaacggcg aatatgaagc
     2041 gttcggtgta cccgcgacga ttcttgctaa cttcctgcgc gaaaatggcg tagtgccgga
     2101 aaaatgcgat cttaacgcca tcctcttcct gctgactccg gcagaagata tggccaaact
     2161 tcagcaactt gtgccctgct ggtacgcttc gaaaaactgc ttgagtccga cgcgccatta
     2221 gcagaagtgc tgccttccat ctacaaacag catgaagagc gctacgccgg ttataccctg
     2281 cgtcagttgt gccaggaaat gcatgatttg tatgcccgcc acaacgtgaa acaactgcaa
     2341 aaagagatgt tccgcaaagc gcacttccca cacgtcagca tgaatccgca agaagccaac
     2401 tacgcctatt tacgcggtga agtggagctg gttcgtctac cggatgccga aggtcgtatc
     2461 gctgccgaag gggcgcttcc ttatcctccg ggtgtgctgt gtgttgttcc gggtgaaatc
     2521 tggggtggtg ctgttttgcg ttacttcagc gctctggaag aagggatcaa cctgctgcca
     2581 ggttttgcac cggagctgca gggtgtctat atcgaagaac atgatggtcg taagcaagtt
     2641 tggtgctatg tcatcaagcc tcgtgatgcg caaagcaccc tgttgaaagg ggaaaaatta
     2701 tgagtcaggc taaatcgaac aaaatgggcg tcgttcagtt aaccatactg acgatggtca
     2761 acatgatggg ctccggtatc atcatgctgc cgacaaagct tgccgaagtt gggacaattt
     2821 caattatctc ctggctggtg acagccgtag gctcaatggc actggcatgg gcattcgcaa
     2881 aatgcggtat gttcagccgt aaatcaggtg gtatgggcgg ttatgccgag tatgctttcg
     2941 gtaaatccgg taactttatt gcgaactata cctacggcgt ctcactgctg attgctaacg
     3001 tcgcgattgc tatttcggcg gttggttacg gcaccgaact gctcggcgcg agtttgtcgc
     3061 cagtgcagat tggtcttgcg accatcgggg tgctgtggat ttgtaccgtg gctaactttg
     3121 gtggtgcgcg cattaccggg caaatcagta gcattaccgt gtgggggggc attattccgg
     3181 tggtaggcct gtgcattatc ggctggttct ggtttagccc gacgctttac gttgattcct
     3241 ggaatccgca tcatgcaccg ttcttcagtg cagtaggttc ttccatcgct atgacgctgt
     3301 gggcttttct tggtctggag tctgcgtgtg cgaatactga tgtagtggaa aacccggaac
     3361 gtaatgtgcc aatcgcggta ctcggcggta cgttaggtgc ggcggtgatt tatatcgtct
     3421 ccaccaacgt gattgcaggg attgtgccta atatggagct ggcaaattca acggcaccat
     3481 ttggcctggc cttcgcacag atgtttacac cagaagtggg taaagtcatt atggcgctga
     3541 tggtgatgtc ctgctgcggt tcgctgcttg gctggcagtt caccattgcc caggtgttta
     3601 aatcttcatc tgatgaaggc tacttcccta aaattttctc ccgtgtaacc aaagtggatg
     3661 caccggtgca gggaatgttg accattgtga ttatccagag cgggttggcg ctgatgacga
     3721 ttagcccgtc gctaaacagt cagttcaacg tgctggttaa cctggccgtg gtgaccaata
     3781 tcattccgta tattctgtca atggcggcat tagtcattat tcagaaggtc gctaatgtgc
     3841 ccccctcaaa agcgaaagtt gcaaactttg ttgcttttgt tggcgcgatg tatagctttt
     3901 atgcgctgta ctcatccggg gaagaagcca tgctgtacgg ttccatcgtg accttccttg
     3961 gttggacact gtatggtctg gtatcaccac gctttgaact gaaaaataaa cacggttaaa
     4021 aaagcaaagt aaaaaagggc gatcttgcga ccgctctttt tttattaaat gtgtttacgc
     4081 gtttttcaga acttcgctaa caatctcaac cgcttctttc tcaatctgct tgcgatgttc
     4141 ttcaccgagg aagctttcgc agtagatctt atatgcgtct tccgtgcctg acggacgcgc
     4201 ggcgaaccag ccgttgtcag tcatcacttt cagaccgcca atagaagcac cgttgcccgg
     4261 agcagcagtc aggcgcgcgg tgatcgggtc acctgccagg gtgctggcgc tcaccatttc
     4321 cggagacagc ttagacagcg ccgctttttg tgcggaagtc gcagctgcct gcaaacggtt
     4381 gtagctcggc gcaccaaagc gttttgccag ttcgttgtag tgttcctgcg ggttcttacc
     4441 ggtgacagcg gtgatttccg ccgccagcag acacataatg atgccgtctt tgtcggtgga
     4501 ccacggcgtg ccgtcgaaac gcaggaagga agcccctgcg ctctcttcgc cgccaaagcc
     4561 gaagctgccg tcgaacagac catcaacaaa ccatttgaaa cctaccggta cttctaccag
     4621 cttacggccc aagtcattga ccacacggtc gatcatcgca gaagaaacca gcgttttacc
     4681 gacagcaaca tctttgcccc actgcggacg atgctggaac aggtagttga tcgccaccgc
     4741 caggtagtgg ttcggattca tcaaacctgc cggagtgacg ataccgtgac ggtcataatc
     4801 cgggtcgtta gcaaacgcca gatcgaactt atcacgcagt gccagcagac ccgccatcgc
     4861 acactcggag gagcagtcca tacggatcgc accgtcttta tcgaggtgca taaagcggaa
     4921 ggtttgatcg acctgatcgt taacgatggt caggttgagg ttgtaatact cgccaatgcg
     4981 cttccagtat tcgataccgg aaccgcccaa cggatcaacg cccagcgtca ggcccgcttt
     5041 ctgaatcgcc gccatatcaa cgatatcggc cagcccttcc acgaacggct gcaccagatc
     5101 ctgctctttc acatagccgg atgccatcgc ttcgtcgagg gagatacgct tcacgccttt
     5161 caggccatcg gccatcagtg cgttggcctt gtcttccacc actttggtga cgttagtatc
     5221 agccgggcca ccatttggcg gattgtactt gataccacca tcttccggcg ggttatggga
     5281 cggtgtaatc acgataccgt ctgccagcgg gccacctttt ttattgtgaa ccaggatggc
     5341 attggaaacg gcaggcgtcg gagtgaagcc attgttttcc tgcacaatga catcaacgcc
     5401 gttcgctgcc agcacttcca gtacggaaat gaacgcaggc tcggacaggg cgtgagtatc
     5461 tttacccaca tagcaagggc cagtgatgcc gtttttcgca cgttcttcag caattgcctg
     5521 agcgatcgcc agaatgtgcg gctcgttaaa gctgtggcgc gctgcactgc cacggtgacc
     5581 ggaagtaccg aatttcaccg cgtgctccgc attccctgct tctggtttca gtacataata
     5641 ttgcgccgtc agttgggcga cgttaatcaa atcactctgt tgtgcaggtt ggcctgcacg
     5701 attgtggatt gccattgctt tgtcctttgt ctgcaacgtt ttagatagtt ccgcaaacct
     5761 tctcaatcaa ttccgccggg aattgcatcg actgcatgat gtgttcgatc atgctgcatt
     5821 tacggccggt gttggtgttg gtgatcaccc aatacggcgt gcctggcaca tgtttcggct
     5881 tggtctgatt accatttttc agcagcgttt gttcatctgc cgcaaagtac acgcgtgtac
     5941 gaccgtgcaa cgattccgtt gcttcggcaa acgcctgggc gtcaagagaa tatagtgtag
     6001 acaacagcag cataaagcga ttgaccgctc gcttttgctc tgcgtattcg tccgaaagca
     6061 gaagttcacg cattgcgcga accttgtctt taatcgtttt gaccggcttc gcttcgacga
     6121 tagcaggtga cgcaacgcga acctctttcg tcaccggagc agcaggctgt gatgcggcgg
     6181 aaaatttcaa catacgccgt aaaatgtcgg atgcgctctc gccgatatgc ttagtgtggc
     6241 tggcaatata gctgtagagt tcatcatcaa cttcaatcgt tttcatctta atccagtgca
     6301 gtgtcttagc tgaatacaag tcgccaagag tatatgaata aatcccggca gcggatagcg
     6361 tcaagcctgg cttgcagaaa aactcggaat aaaccttatt tgttgcagcc ctgtggcaca
     6421 atggtcaaca taatacccta acccgacaac gcgaaaaaaa gaactttgcc atgaaattaa
     6481 atatccgcgc gcaaactgca caaaaccagc acaataattc tcccatcgtt cttgtccatg
     6541 gtctgtttgg tagcctcgac aaccttggcg tactggctcg cgatctggta aacgatcaca
     6601 atatcatcca ggttgatatg cgtaaccacg gtctttcacc gagagagccg gtaatgaatt
     6661 acccggcgat ggcgcaggat cttgttgata ccctggatgc actgcagatc gacaaagcaa
     6721 catttatcgg tcactccatg ggcggtaaag cggtaatggc acttactgca ctggcccccg
     6781 atcgcatcga taaactggtg gcgatcgata tcgcaccggt cgactatcac gtacgccgtc
     6841 atgatgagat tttcgcagct atcaacgcgg tcagtgaatc ggacgcacaa actcgccagc
     6901 aagcagcagc aataatgcgc cagcatctta atgaagaagg ggtgattcag tttctgttga
     6961 aatcttttgt tgacggggag tggcgcttta acgtgccggt attgtgggat cagtatccgc
     7021 atattgtggg ttgggagaaa atcccggcat gggatcaccc tgccctgttt atccctggcg
     7081 gcaattctcc gtatgttagc gagcagtacc gtgatgattt actggcacaa tttccacagg
     7141 cacgggcgca tgtgattgca ggcgcgggtc actgggtcca tgctgaaaaa ccggatgcgg
     7201 tattacgcgc tatccgtcgc tatctcaatg attaacgcaa ctgattaaaa acccagcgtc
     7261 cgcgtcgctt tccgcgcgtg ggcgttggcg tgactcatcg cgctgatgta ttatggcgcg
     7321 ctatccatcc gggcagaggc ctggctgatt gtttcccccg aagtcaccaa gatcatggcc
     7381 aaagaacaaa cggaccgtac gacattagat ctgttcgcgc acgagcgtcg accgggacga
     7441 ccgaaaacta atccgctttc gcgcgatgaa cagctgcgta ttaataaacg caaccagcta
     7501 aaacgcgaca aagtacgtgg ccttaagcgt gtcgaactga agctgaacgc ggaagctgtc
     7561 gaggcgctga acgagctggc ggaatcgcgt aatatgagcc gtagcgaact gatcgaagag
     7621 atgttaatgc agcaactggc ggcgctgcgt agtcagggca tcgtttaaat ttccactttc
     7681 atgtagcaca gtgtgcagtc ctgctcgttt gctgattccg caagactgcc tgttctgcta
     7741 tgattgcctt tatccgtggg caattttcca cccccatttc aataagtttc aagaggttat
     7801 ttcactcatg gctatcactg gcatcttttt cggcagcgac accggtaata ccgaaaatat
     7861 cgcaaaaatg attcaaaaac agcttggtaa agacgttgcc gatgtccatg acattgcaaa
     7921 aagcagcaaa gaagatctgg aagcttacga cattctgctg ctgggcatcc caacctggta
     7981 ttacggcgaa gcgcagtgtg actgggatga cttcttcccg actctcgaag agattgattt
     8041 caatggtaaa ctggttgcgc tgtttggttg tggtgaccag gaagattacg ccgaatattt
     8101 ctgcgacgca ttgggcacca tccgcgacat cattgaaccg cgcggtgcaa ccatcgttgg
     8161 tcactggcca actgcgggct atcatttcga agcatcaaaa ggtctggcag atgacgacca
     8221 ctttgtcggt ctggctatcg acgaagaccg tcagccggaa ctgaccgctg aacgtgtaga
     8281 aaaatgggtt aaacagattt ctgaagagtt gcatctcgac gaaattctta atgcctgatg
     8341 tgatgcggcg tagactcatg tctacgccgt attaatagat aatgccaatc aaaataattg
     8401 ctacaaattt gtaacttttg ctgttgtacc tatacaatgt cccagtgttc aagtggcctt
     8461 gccgttgtaa atgtaagctg tgccacgttt ttattaacaa tatttgccag ggacttgtgg
     8521 ttttcattta ggcgtggcaa ttctataatg atacgcatta tctcaagagc aaattctgtc
     8581 acttcttcta atgaagtgaa ccgcttagta acaggacaga ttccgcatga ctgataacaa
     8641 taccgcccta aagaaagctg gcctgaaagt aacgcttcct cgtttaaaaa tcctggaagt
     8701 tcttcaggag ccggacaacc atcacgtcag tgcggaagat ttatacaaac gtctgatcga
     8761 tatgggtgaa gaaattggtc tggctacggt atatcgcgta ctgaaccagt ttgacgacgc
     8821 tggtatcgtc acccgccaca attttgaagg cggtaaatcc gtatttgaac tgacacagca
     8881 acatcaccac gatcacctga tttgcctcga ctgcggcaag gttatcgaat ttagtgatga
     8941 ttccatcgaa gcgcgtcagc gtgaaattgc cgcaaaacat ggcattcgcc tgactaacca
     9001 cagtctctat ctttacggtc actgtgccga aggcgattgc cgcgaagatg agcacgcgca
     9061 cgaaggcaaa taagccagcc tgaacgagaa aagccaacct gcgggttggc ttttttatac
     9121 aagggaaata agaaaagcga gagttacagc tctcacttat ttgttattac tgcgtatttc
     9181 ctgccagacc ttatcacaat cggctttcac cgcctgatca tttccggttt gcgttgcacg
     9241 cagacactgc tgataatcca gtacgcgtac actttcctgg tcggcaaact gctgatgttg
     9301 tttctctttc ttcagcacgt ttaacacgct ctggcaggct tcaatttttt ctggcgaacc
     9361 ttgcgcggtg ttgatacagg cgctgtatgc ctctttcaga cggctgtctt cctgtggtgc
     9421 ggttgattgc gcacaagcta ccagccccga tgccatcatg gcgatgagaa tcagtttttt
     9481 cattgaatgc ggcaggcaca aaacctgccg cgtcgggcat cagaagatgg tgaatggtgc
     9541 gattaccata aatttcacgt cacgctcatc ctggaagatg ttaccgtaac cgccgcccca
     9601 gcttgggatg tcggagtggt tgtcgtattc ggtgaagtgc agtttgaaca tcgtgccttt
     9661 ggcgcgaccg tcctgaatgg tgtagaccgc atccaggctg taggcagact cttcaatagt
     9721 ccggtttttg tcgtagtacg catccggatt gctctgccag gtcgcaggtt tagcatccca
     9781 tgcgtaaacg taggaagcgc cgatggcgaa gcctggaaga ttccagtttt tcaggtcata
     9841 catcgcactg aagaagaccg ctttttcgcc gttagcgttg aagtcggaac ggttatccca
     9901 ccagatatcc aggcggccgt tggaggaagc gtaggttggg gtcatacgtt gcaggaagta
     9961 tccctgctga ctgtcagcct taatccaggt gccttcgagg cgcaaatcca ctacgtcagc
    10021 cgcccggtaa ccaaaggtca acgcttgcag ccaggcggtg ccgtcataaa gatcgttgac
    10081 gctgcgatcg tcaactttat cgcgggtacc atagaactgg tagctggtgg ttaacgggct
    10141 accggcgata tcaaatttgt agctggcttt ggcaaaatat tgatcgatat acccttccgc
    10201 ctgaccaaat gccgcttcca gtacgaagtt atttttgaag tcgtatttcg caccgatgga
    10261 gtgcagataa tcaactttgg tggttttatc gttctgataa aactcatcca tttccagatg
    10321 ccacggcgct ttgtattcgt tggtccacat gtaggagaaa ctcaaggcac cagcatcgcc
    10381 gtaatcaaaa ttcgccccgg cttccgcccc ctgataagta cccggcataa agctccagtg
    10441 cggcgctaac agcgtttgac cggttggctg aatgtaacct gccctcgccc aaaccggacc
    10501 gtatttaaat ttggccgcag ctttatacag gcttataccg cttttgtcgc cggaccagtc
    10561 ttcgtcatag gctttattac tttttgaaaa cgcgatttcg ttcgggtggg agctgtcgcc
    10621 gttttccgcc atttcaatcg ccgtaaacgc agcaatatca aggccgaaca tatcagcagc
    10681 ataaccggac tgaaaatcga ggttggcatt ccaggtggag tgagaaaggt tggttttgta
    10741 tttgtcgccg tcggtaacat ctttacggtc gcgttcacgc tgccagtaat agataccgcc
    10801 ggttaaggtt gaatcgtcga tgaatccttc ggcgcgagcc tccggcattg ccataaagcc
    10861 cgacattgct gtaacaccgg caatagccag cgccagcgta ctacgtttgc cactaaacgt
    10921 acgcatgggt taatcctctt tgacgtataa attgctgcac caaaggtgaa cagcgaaaaa
    10981 aaataaaaat taaaaaagta gcgttgagaa aagtctcgct gactacagga acagactatt
    11041 tttcgcgacg cgaattatca atctctttct gtaaccagaa tgtaagaaca tgagcaaccg
    11101 cacatatttt gatgattttt gttacaaggc ttaagtttgc ggttgaaagg cttgcagaag
    11161 aataaaaacg
//
LOCUS       DAZPAB010000124        10982 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZPAB010000124 DAZPAB010000000
VERSION     DAZPAB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10982
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.124"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(198..740)
                     /gene="fimA"
                     /locus_tag="ACHUPG_002840"
     CDS             complement(198..740)
                     /gene="fimA"
                     /locus_tag="ACHUPG_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFR0862378.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            1221..2087
                     /gene="folD"
                     /locus_tag="ACHUPG_002841"
     CDS             1221..2087
                     /gene="folD"
                     /locus_tag="ACHUPG_002841"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR0862379.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            2089..2301
                     /gene="ybcJ"
                     /locus_tag="ACHUPG_002842"
     CDS             2089..2301
                     /gene="ybcJ"
                     /locus_tag="ACHUPG_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR0862380.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            2409..2918
                     /locus_tag="ACHUPG_002843"
     CDS             2409..2918
                     /locus_tag="ACHUPG_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0862381.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVLAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(2954..4339)
                     /gene="cysS"
                     /locus_tag="ACHUPG_002844"
     CDS             complement(2954..4339)
                     /gene="cysS"
                     /locus_tag="ACHUPG_002844"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR0862382.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            4513..5007
                     /gene="ppiB"
                     /locus_tag="ACHUPG_002845"
     CDS             4513..5007
                     /gene="ppiB"
                     /locus_tag="ACHUPG_002845"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR0862383.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            5010..5732
                     /gene="lpxH"
                     /locus_tag="ACHUPG_002846"
     CDS             5010..5732
                     /gene="lpxH"
                     /locus_tag="ACHUPG_002846"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR0862384.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            5850..6359
                     /gene="purE"
                     /locus_tag="ACHUPG_002847"
     CDS             5850..6359
                     /gene="purE"
                     /locus_tag="ACHUPG_002847"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR0862385.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            6356..7423
                     /gene="purK"
                     /locus_tag="ACHUPG_002848"
     CDS             6356..7423
                     /gene="purK"
                     /locus_tag="ACHUPG_002848"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR0862386.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(7560..8453)
                     /locus_tag="ACHUPG_002849"
     CDS             complement(7560..8453)
                     /locus_tag="ACHUPG_002849"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR0862387.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(8450..9097)
                     /locus_tag="ACHUPG_002850"
                     /pseudo
     CDS             complement(8450..9097)
                     /locus_tag="ACHUPG_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            9100..10332
                     /gene="allC"
                     /locus_tag="ACHUPG_002851"
     CDS             9100..10332
                     /gene="allC"
                     /locus_tag="ACHUPG_002851"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFR0862388.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            10343..>10982
                     /gene="allE"
                     /locus_tag="ACHUPG_002852"
     CDS             10343..>10982
                     /gene="allE"
                     /locus_tag="ACHUPG_002852"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFR0862389.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2613 a         2897 c         2850 g         2622 t
ORIGIN      
        1 ctgacggcaa aaataaggag tataaagaaa tgattgcttt ggcttcttac aacacatcat
       61 tcccttttca tttaataaag cggcatgata caaagtgaac gtggtttcga tttacattgc
      121 caccataccg cttcagacaa ccggtacatt actttgttat aagacgtgat atataattca
      181 aaacaacgtg gttttgatta ttcgtatctc ataataaaag tcgcgtcagc atttgcttgc
      241 cctgctgatg cacttgtacc ggtgccttta taacgtgcag aaaaatgaag aacgttggtc
      301 ccggggatca agttctggtt ggttgagaag ctattcccat caggtttgag aattgcggat
      361 gtattatcaa gtatttcaat accgacaccc gttgctgttg ttgtatttgt actggataca
      421 atggccaata aattatcatt gattgcatca gcctgaccag aaaatgcaac ggcagcatta
      481 gcggcaacaa caggatcgca gtcgttcaac tgaatggtga atggaattaa tgcagaggta
      541 ttaccaacag cattgaaaat atcggtacga tattgaccga gtgtgacaac ctggtctgcc
      601 gagtcagtat tcactgaaca ggcagcattc accagttcgc cttcaaagtg aattgtaccg
      661 ccatctacaa cggcagcata actacccgta gcggcgaata gtgcggcagc cagcgcagac
      721 gaaataaatc ttaatttcat atatattcct tcaatctcat ttatcgactc cacatccgta
      781 tataaccgat tactttattt aagacactga tagtagtaaa attccttttt atcctctaag
      841 aatgtcttaa ttgaaaatat gcactctatt ctaaaaaata gagagccccg ttagatgaat
      901 acttccgcgc aaaatatatt caccacaaat atagacctga agcggtaaat taccaggctg
      961 aaaattcttt ttatattgtc aggtatttct taaattatct taatccttaa acaaggaaat
     1021 aaatcagttc cagatttaca acgccatcat gtacgaaaaa tgaagctttc aatctcagcg
     1081 acctcagcga cggtgcgcct aaccttcgca agaggtcgct tcacgcgata aatctgaaac
     1141 gaaacctgac agcgcgcccc gcttctgaca aaatagacgc atccccttcg acctacgtaa
     1201 cagatggaat cctctctctg atggcagcaa agattattga cggtaaaacg attgcgcagc
     1261 aggtgcgctc tgaagttgct caaaaagttc aggctcgtat tgcagccgga ctgcgggcac
     1321 caggactggc cgttgtgctg gtgggtagta accctgcatc gcaaatttat gtcgcaagca
     1381 aacgcaaggc ttgtgaagaa gtcgggttcg tctcccgctc ttatgacctc ccggaaacca
     1441 ccagcgaagc ggagctgctg gagcttatcg atgcgctgaa tgccgacaac accatcgatg
     1501 gcattctggt tcaactgccg ttaccggcgg gtattgataa cgtcaaagtg ctggaacgta
     1561 ttcatccgga caaagacgtg gacggtttcc atccttacaa cgtcggtcgt ctgtgccagc
     1621 gcgcgccgcg tctgcgtccc tgcaccccgc gcggtatcgt cacgctgctt gagcgttaca
     1681 acattgatac cttcggcctc aacgccgtgg tgattggcgc atcgaatatc gtcggtcgcc
     1741 caatgagcat ggaactgttg ctcgcaggtt gcaccaccac cgtgactcac cgcttcacta
     1801 aaaatctgcg tcatcacgta gaaaatgccg atctgttgat cgttgccgtt ggcaagccag
     1861 gctttattcc cggtgactgg atcaaagaag gcgcaattgt gattgatgtc ggcatcaacc
     1921 gtctggaaaa tggcaaagtt gtgggcgacg tcgtgtttga agacgcggct aaacgcgcct
     1981 catacattac gcctgttccc ggcggcgttg gcccgatgac ggttgccacg ctgattgaaa
     2041 acacgctaca ggcgtgcgtt gaatatcatg atccacaggg tgagtaacat ggcgacattt
     2101 tctttaggta aacatccgca cgttgagctg tgcgacttgc tgaaactgga aggctggagc
     2161 gaaagcggcg cgcaggcgaa aatcgcgatt gccgaaggcc aggtgaaagt cgacggtgcg
     2221 gttgaaacgc gcaaacgctg caaaatcgtc gccggtcaga cagtgagttt tgcaggacac
     2281 agcgtacagg ttgttgcctg atccctttcc cggcctgtat caacaggccg gttctctttt
     2341 cagacttttg ccctctacgc cccgttcatc catgctgata gcatcgacat tttgccgaag
     2401 cgaccatcat gccaaccgtt attacgcacg ccgccgttcc cctttgcatt ggtttagggt
     2461 tgggttcgaa aggtctgtta tttgccggaa tcatcctggc gatgctgccc gatgccgacg
     2521 tattgtcgtt taaatttggc gttgcttacg gcaatgtttt tggtcatcgc ggttttaccc
     2581 attcgctggt gcttgcgttt gttgtcccgc tcttatgtgt gctgattgga cgtcgatggt
     2641 tcagggctgg gctgattcgc tgctggctgt ttttaaccgt ctcgttgcta tcgcacagct
     2701 tactggattc ggtaaccact ggcggtaagg gcgttggctg gctgtggccg tggtcagatg
     2761 aacgcttttt cgctccctgg caggtgatta aagtcgcgcc gtttgcattg tctcgttaca
     2821 ccacagcgta cgggcatcag gtgattattt ccgaattgat gtgggtgtgg ttgccgggga
     2881 tgctactgat gggaatgttg tggtggcgca gacgataacc ggatgcgaaa actcgcatcc
     2941 ggcaattgcg caattactta cgacgccagg tggtcccttg cgggccatct tccagcacga
     3001 tccccatctc gttaagacgg tcacgtgccg catctgccgc cgcccagtct ttcgctttac
     3061 gggcatccag acgctgttga attaacgctt caatctcagc cacttcgctg tcgtctgcct
     3121 gcgcgccgct ttgcaggaac gcttccggtt cttgctccag caggcccaat acagcggaaa
     3181 gtttacgcag gtgagatgcc attgcattcg ccgctgccat atcttctgct ttcagacggt
     3241 tcacttcacg cgccatatca aacagcacgg aataggcttc cggggtgttg aaatcgtcgt
     3301 ccatcgcttc aataaagcgc gcttcaaacg cttcgccacc ggcaggcgca acggttttat
     3361 ctgtgccgcg cagcgcagtg tagaggcgct ccagcgccgc acgcgcctgc ttcaggttct
     3421 cttcgctgta gttcagctgg ctgcgatagt ggcccgacat caggaagtaa cgcacggttt
     3481 ccgcgtcgta gtatttcagc acatcgcgca cggtaaagaa gttacccagc gatttggaca
     3541 tcttctcgcg gtcaaccatc accattccgg agtgcatcca gtagttcaca tactgaccat
     3601 catgggcaca ggtggactgc gcgatttcgt tctcgtggtg cgggaacatc aggtctgaac
     3661 cgccgccgtg gatatcaaag tggttaccca gctgcttgca gttcatggcc gaacattcaa
     3721 tgtgccagcc aggacgaccc gcgccccacg gagacggcca gctcggttcg ccctctttcg
     3781 acatcttcca cagaacgaag tccatccggt tgcgtttgtc atcaaccacg tcaacgcgcg
     3841 cgccagcctg cagctggtcg agatcctgac gcgacagcac gccataagtt ggatcggtcg
     3901 ggacgtcgaa catcacgtcg ctgttgtccg ccacataagc gtgacctttg gcgatcagtt
     3961 gttcagtgag ttcaataatt tctgcgatat ggtgcgtcgc gcgcggctcc atatccgggc
     4021 gcagaatgtt caaagcatca aaatctttgt gcatttcggc gatcatgcga tccaccagcg
     4081 ccacaaagct ttcgccattt tcattggcgc gtttgatgat tttgtcgtcg atatcggtaa
     4141 tgttgcgcac atacttcagt ttatagccga ggaaacgcag atagcgcgca accacgtcaa
     4201 aagcaacaaa ggtacgcccg tgaccgatat gacagagatc gtaaacggtg attccacaca
     4261 cgtacatgcc gacttccccg gcgtgaatag gcttaaattc ctctttttgg cgtgtcagag
     4321 tattgaagat ttttagcatc gaagattccg tttagacatg tgtgggtaat tgagttgcgt
     4381 ataataccca tatttcccgc gcgaatcagc atacattgcg tgatgatccg atcgtgcggt
     4441 tatgctataa caccacccta tatatgaccc gaactgggtt gaagcaccaa tcaaacggaa
     4501 caggatgcaa aaatggttac tttccacacc aatcacggcg atattgtcat caaaactttt
     4561 gacgataaag cacctgaaac agttaaaaac ttcctggact actgccgcga aggtttttac
     4621 aacaacacca ttttccaccg tgttatcaac ggctttatga ttcagggcgg cggttttgaa
     4681 ccgggcatga aacaaaaagc caccaaagaa ccgatcaaaa acgaagccaa caacggcctg
     4741 aaaaataccc gtggtacgct ggcaatggca cgtactcagg ctccgcactc tgcaactgca
     4801 cagttcttca tcaacgtggt tgataacgac ttcctgaact tctctggcga aagcctgcaa
     4861 ggttggggct actgcgtgtt tgctgaagtg gttgacggca tggacgtggt agacaaaatc
     4921 aaaggtgttg caaccggtcg taacggtatg caccaggacg tgccaaaaga agacgttatc
     4981 attgaaagcg tgaccgttag cgagtaatcg tggcgacact ctttattgca gatcttcatc
     5041 tctgcgtgga agaaccggcg atcaccgccg gttttctgcg ttttttagcg ggggaagccc
     5101 gcaaggccga cgcgctgtat attcttggcg atctgtttga agcatggatt ggcgacgacg
     5161 atcccaaccc actccatcgc cagatggcgg cagcgatcaa agcggtgtcc gattccggcg
     5221 ttccctgtta tttcattcat ggcaaccgtg attttctgct cggcaaacgc tttgcccgtg
     5281 aaagcggcat gacattattg ccggaagaaa aggtgctcga actttatggt cgccgggtgt
     5341 tgattatgca tggcgacacg ctgtgcaccg atgacgcggg ttatcaggct tttcgcgcca
     5401 aagtccacaa accctggctg caaatgctat tcctcgccct gccgttgttt gtgcgcaaac
     5461 gcattgccgc gcgaatgcgc gcgaacagca aagaagccaa cagcagtaaa tcgctggcga
     5521 tcatggacgt taaccaaaac gctgtggtca gtgcgatgga aaaacatcag gtgcaatggc
     5581 taatccacgg gcatacccat cgcccggcgg tgcatgaact tatcgccaac cagcaacctg
     5641 cttttcgcgt ggtactgggt gcctggcata cggaaggttc gatggtgaaa gtcacggctg
     5701 atgacgttga gctgattcat tttccgtttt aaaaaaccca caactttgct gatttcacag
     5761 ccacgcaacc gttttccttg ctctctttcc gtgctattct ctgtgccctc taaagccgag
     5821 agttgtgcac cacaggagtt ttaagacgca tgtcttcccg caataatccg gcgcgtgtcg
     5881 ccatcgtgat ggggtccaaa agcgactggg ctaccatgca gttcgccgtc gaaatcttcg
     5941 aaatcctgaa tgtcccgcac cacgttgaag tggtttctgc tcaccgcacc cccgataaac
     6001 tgttcagctt cgccgaaagc gccgaagaga acggttatca ggtgattatt gcgggcgcag
     6061 gcggcgcagc gcacctgcca ggcatgattg ccgccaaaac gctggtgccg gtgctgggcg
     6121 tgccagtaca gagcgccgca ctgagcggtg tcgatagcct ctactccatc gtacaaatgc
     6181 cgcgcggcat tccggtgggt acgctggcga ttggtaaagc tggcgcggca aacgcggcat
     6241 tactggcagc acaaattctc gcgactcacg ataaagagct acaccagcgt ctgaatggct
     6301 ggcgcaaagc ccagaccgac gaagtgctgg aaaacccgga cccgcgaggt gcggcatgaa
     6361 acaggtttgc gtcctcggta acgggcagtt aggccgtatg ctgcgtcagg caggcgaacc
     6421 gttaggcatt gctgtctggc cggtcgggct ggacgctgaa ccggcggcgg tgccttttca
     6481 acaaagcgtg attaccgctg agatcgaacg ctggccggaa accgcattaa cccgcgagct
     6541 ggcgcgtcat ccggcctttg tgaaccgcga tgtgttcccg attattgctg accgtctgac
     6601 tcagaaacag cttttcgata agctccacct gccgaccgcg ccgtggcagt tacttgccga
     6661 tcgcagcgag tggcctgcgg tgtttgatcg tttaggtgaa ctggcgattg ttaagcgtcg
     6721 cactggtggc tatgacggtc gcggtcaatg gagtttacgc gccaatgaaa ccgaacagtt
     6781 accggcagag tgttacggtg aatgtattgt cgagcagggc attaacttct ctggtgaagt
     6841 atcgctggtt ggcgcgcgcg gctttgatgg cagcaccgtg ttttatccgc tgacgcataa
     6901 cctgcatcag gacggtattt tgcgcaccag cgtcgctttt ccgcaggcca acgcacagca
     6961 gcaggcgcaa gccgaagaga tgctgtcggc gattatgcag gagctgggct atgtgggcgt
     7021 gatggcgatg gagtgttttg tcaccccgca aggtctgctg atcaacgaac tggctccgcg
     7081 tgtgcataac agcggtcact ggacacaaaa cggtgccagc atcagccagt ttgagctgca
     7141 tctgcgggcg attaccgatc tgccgttacc gcaaccagta gtgaataatc cgtcggtgat
     7201 gatcaatctg attggtagcg atgtgaatta tgactggctg aaattgccgc tggtgcatct
     7261 gcactggtac gacaaagaag tccgtccggg gcgtaaagtg gggcatctga atttgaccga
     7321 cagcgacaca tcgcgtctga ctgcgacgct ggaagcctta atcccgctgc tgccgccaga
     7381 gtatgccagc ggcgtgattt gggcgcagag taagttcagt taagcaggtg ctcatttgat
     7441 tgccggatgc ggcgtaaacg ccttatccgg cttaccgctc cggtacccat tgtaggcctg
     7501 ataagacgcg tcaagcgtcg catcaggcat ggtgcaccga ttgccggatg ctctcatcac
     7561 tacagcgaaa tacaggtccc cgcttcgccc gccagcgtct cttcaattcg cgataacgcc
     7621 ccaatccacg cgggtttact acggcttctg acataaccac tcaccgccgt taccttcggc
     7681 cccatcgaac catcggcttt ggcaaatggc gctaactcat ccggtgtggc atggcgaatg
     7741 gcacgttgct gcggcgttcc ccagttttca tataccgcat cagcatcggt gaggatcacc
     7801 agtccatcgg cattaatctg ctcggcgagc aacgcagcgg cgagatcttt atcaatcacc
     7861 gcttcactcc ctgccccatc ttccgtcaca ggcacaccgc cgccgccact gcaaatcacc
     7921 acatgcccct ctttgagcaa caactcgatg gcttcgctgt cgagaatttt acgcggttgc
     7981 ggagacgcca ccacccggcg caaatattta ccatcacgtt tcatctgcca gccgtaagcc
     8041 gcttccagtg cctcttgttc ttctggctga taaaccggac caataaattt ctctggctgc
     8101 aaaaacgccg gatcatcagg cgaaacctca atgcgcgtca gcaccgtcgt aacaggcggc
     8161 atctgcggct gtgcgctcaa gctctgcgcc agcatatagc caatcatccc ctggctttcc
     8221 gccaccagta catccagcgg atacggttct acctctttcc acgccagatt ctgtaacgcc
     8281 agcagcccca cctgcggccc gttgccgtga acaatcgcca gccgataaga acgggccagg
     8341 cgtgccagcg cgggtacagc actggcgata ttgcgatatt gattttccgc cgtcagcgcc
     8401 tcaccgcgct ggagtaaggc gttgccccca agagctacaa ccagtgtttt catggttttc
     8461 cttgtaataa ttgttggcca agccagaacc ccagcagcgt atctgcccct gacgtatgcc
     8521 ccagcgccag cagcgaatca atcgcaacag cggtacgttt cgggcaactc agagcatgga
     8581 caaagtgcag gagtggcgag gcgaaatatc cttgcgcggc ataacgtaaa taactgacgc
     8641 tcaccgctgt ggtaacgagt tgaagattgt cggaacaggc aaaaaacggg cgaccggcgc
     8701 gcgcatctaa agcgccataa taccaggccg ccagcagcat tccgctcaac gtgtcatcat
     8761 gacttggcgt taatccgggg cctttaccca gccagtgccg ccagtcggtc ttaacgccat
     8821 tgagcgcggc ctgaaaacag tgacgaaact ggcgtaattc agcgggcagc ggatcgcttg
     8881 ccgccatcgc cagtggcccg aaaagtccgg tttcctccgc gcgttgcatc catgcagctg
     8941 caagcggttg aggatgcgaa ggcggcgtaa tacgcagcaa acaataacgc tgtggctgtt
     9001 taaccgtgaa acgcccgagg cgaatccctt gagccacaac ctgtggtcgt tcattgccgc
     9061 ataatccacc gcataacgca tcgaattgcg tgcggcgaaa ttacacattc ccgtcaagct
     9121 atagaagaaa cgctgccctg gctttcctct tttggcgctg acccaacggg tgggatgacc
     9181 cgtttacttt attcgccgga atggctggaa acccagcagc aatttaaaaa aagaatggca
     9241 gcaagtgggc tggaaacacg tttcgatgaa gtggggaatt tatacggtcg cctgagtggc
     9301 accgaatatc cacaggaagt ggttctgagc ggttcgcata tcgataccgt ggttaacggc
     9361 ggtaacctcg acgggcaatt cggcgcgctg gcggcgtggc tggcaattga ctggttgaaa
     9421 acgcaatacg gcgcaccgtt acgtacggtc gaagtggtgg cgatggcaga agaagaaggc
     9481 agccgcttcc cgtatgtctt ctggggcagt aaaaatatct ttgggctggc gaatcctgac
     9541 gacgtgcgga atatctgtga tgccaaagga aatagttttg ccgatgcgat gaaggcttgc
     9601 ggatttactc tgccgaacgc cccactaact ccgcgtcagg ctataaaagc ctttgttgaa
     9661 ctgcatattg aacagggctg tgtgctggaa agtaatgggc aatcaattgg cgtggtgaat
     9721 gcaattgtcg ggcagcgtcg ctatacggtg acgctgaacg gcgaatcaaa ccatgcaggc
     9781 accacgccga tgggttatcg tcgtgatacg gtttacgctt tcagtcgcat ttgccatcag
     9841 tcgatcgaaa aagcgaaaaa gatgggcgat ccgctggttc tgacctttgg caaagtagag
     9901 ccgcgcccga atacggtaaa tgtggtgccg ggtaaaacca cgttcaccat tgattgtcgt
     9961 cataccgacg ctgccgtgct gcgcgatttc acccaacagt tagaaaacga catgcgggcg
    10021 atttgcgatg aaatggacat tggtattgat atcgatttat ggatggacga agaacccgtg
    10081 ccgatgaata aggagctggt cgccaccctg acagaattgt gtgaaagaga aaaactgaat
    10141 taccgggtga tgcacagtgg tgccgggcac gacgcgcaaa ttttcgcgcc tcgcgtacca
    10201 acctgcatga tttttatccc cagcatcaac gggatcagcc ataacccggc ggaacgcacc
    10261 aatattactg accttgccga aggggtcaaa acgttggcac tcatgcttta tcaacttgcc
    10321 tggcaggaat aaggagtcat aaatgggata tttaaataac gtcaccggtt accgcgaaga
    10381 tttactggct aaccgtgcga ttgttaaaca cggtaatttc gcactgttaa ccccggatgg
    10441 tctgataaaa aatattattc cggactttga aaattgtgac gcgacaatcc tctccacgcc
    10501 aaagctgggt gcctcttttg ttgattatct ggtcacactg catcaaaacg gtggcaacca
    10561 acagggcttc ggtggcgaag gcattgaaac gttcctgtat gtgatctctg gaaatatcac
    10621 tgccaaagcc gaaggcaaaa catttgcctt aagcgaaggt ggctatcttt attgcccacc
    10681 aggctcctta atgacgtttg ttaacgccca ggccgaagac agccaaatct ttttatataa
    10741 gcgccgctat gttccggtag aaggccatgc accgtggctg ttttctggca atgccagcga
    10801 actggaacgc attcactatg aaggtatgga tgatgtgatc ctgctggatt ttctgcccaa
    10861 agagttaggt tttgatatga acatgcatat cctctctttt gcaccaggtg ccagccacgg
    10921 ttatatcgaa acacacgttc aggaacatgg tgcctatatt ctttccggtc agggggttta
    10981 ta
//
LOCUS       DAZPAB010000125        10981 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZPAB010000125 DAZPAB010000000
VERSION     DAZPAB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.125"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(153..1817)
                     /gene="glnS"
                     /locus_tag="ACHUPG_002853"
     CDS             complement(153..1817)
                     /gene="glnS"
                     /locus_tag="ACHUPG_002853"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR0862390.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2020..3966)
                     /gene="nagE"
                     /locus_tag="ACHUPG_002854"
     CDS             complement(2020..3966)
                     /gene="nagE"
                     /locus_tag="ACHUPG_002854"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR0862391.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            4299..5099
                     /gene="nagB"
                     /locus_tag="ACHUPG_002855"
     CDS             4299..5099
                     /gene="nagB"
                     /locus_tag="ACHUPG_002855"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR0862392.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5159..6307
                     /gene="nagA"
                     /locus_tag="ACHUPG_002856"
     CDS             5159..6307
                     /gene="nagA"
                     /locus_tag="ACHUPG_002856"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR0862393.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6316..7536
                     /gene="nagC"
                     /locus_tag="ACHUPG_002857"
                     /gene_synonym="nagR"
     CDS             6316..7536
                     /gene="nagC"
                     /locus_tag="ACHUPG_002857"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR0862394.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7584..8336
                     /gene="nagD"
                     /locus_tag="ACHUPG_002858"
                     /pseudo
     CDS             7584..8336
                     /gene="nagD"
                     /locus_tag="ACHUPG_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            complement(8387..8485)
                     /locus_tag="ACHUPG_002859"
                     /pseudo
     CDS             complement(8387..8485)
                     /locus_tag="ACHUPG_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8733..10397
                     /gene="asnB"
                     /locus_tag="ACHUPG_002860"
     CDS             8733..10397
                     /gene="asnB"
                     /locus_tag="ACHUPG_002860"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR0862395.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10776..10852
                     /locus_tag="ACHUPG_002861"
     tRNA            10776..10852
                     /locus_tag="ACHUPG_002861"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10810..10812,aa:Met,seq:cat)
     gene            10861..10945
                     /locus_tag="ACHUPG_002862"
     tRNA            10861..10945
                     /locus_tag="ACHUPG_002862"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10895..10897,aa:Leu,seq:tag)
BASE COUNT         2739 a         2833 c         2776 g         2633 t
ORIGIN      
        1 ccggcctaca aaagcacgca aattcaatat attgcagaga tcatgtaggc ctgataagcg
       61 aagcgcatca ggcaatttag cgtttgaaat ttattgtcta tttattgccg gatgcgacgt
      121 aaatgcctca cccggcttgg tagaacttaa aattactcgc ctactttcgc ccaggtatca
      181 cgcagcccaa cggtgcggtt aaataccggt ttttcggccg tggaatggcg gctgtcgagg
      241 cagaagtaac cttcacgctc aaactggaat gctttacccg caaccgcatc tttcagcgac
      301 ggttcagcaa agccctgttt gatcaccagc gattccgggt taatcaccga caggaaatca
      361 tccgcagcac ctgggttagg cacgctgaac agacgatcat acaaacggat ttcaaccggc
      421 agcgcatgtg ccgcgctcac ccagtgaata acacctttga ctttacgacc atctgccgga
      481 tctttgctta aggtgtcggc gtcataagta cagaagatgg tggtgatatt accttcggca
      541 tctttctcca cgcgttcagc cttaatgacg taagcattac gcagacgcac ttctttaccc
      601 agcaccagac gtttgtactg cttgttagct tcttcgcgga aatcggcgcg atcgatccaa
      661 atttcaccgc taaacggcac ctgacggctc cccatttccg gtttgttcgg atggttcggc
      721 atggtgacca tttcgccttc gccctgatag ttttcgataa ccagtttcac cggatcgata
      781 accgccattg cgcgcggcgc attttcgttg agatcttcac ggatgcagga ttccagcgac
      841 gccatctcaa tggtgttgtc ctgcttggtc acgccgatgc gtttgcagaa ctcacgaata
      901 gaagccgcag tgtaaccacg acgacgcaga ccggaaatgg tcggcatacg cgggtcatcc
      961 cagccttcaa cgtgcttgtc ggtcaccagc aggttcagct tacgcttgga catcacagtg
     1021 tattccagat tcaggcgcga gaattcgtac tggcgcggat gaacaggaat ggtgatgtta
     1081 tccagtaccc agtcgtacag acgacggttg tcctggaact caagcgtaca cagagagtgc
     1141 gtaatacctt ccagcgcatc gctgatgcaa tgggtgaagt cgtacatcgg gtagatgcac
     1201 cacttgttgc cagtctggtg gtgttcagca aatttaatgc ggtacagcac cggatcgcgc
     1261 attacgataa atggcgacgc catgtcgatt ttcgcacgca ggcaggcttt accttcttca
     1321 aaaccaccgg cacgcatttt ttcgaacagc gccaagttct cttcaacgct gcggtcgcgg
     1381 tacgggctgt ttttacccgg ctgcgtcagg gtaccgcggt attcgcggat ctgttccggt
     1441 gtcagttcat caacgtacgc caggccttta ttgatcagtt cgatcgcata ggcgtggagc
     1501 tgatcaaaat aatcggagga gtaacggacg ttaccagacc agtgaaaacc taaccactct
     1561 acgtcgtttt tgatcgaatc gacatactcg atatcttctt ttaccgggtt agtgtcgtcg
     1621 aaacgcaggt tgcactggcc tttatagtcc tgggcgatcc cgaagttcag gcagatagat
     1681 ttcgcatggc caatatgcag atagccattc ggctccggcg ggaaacgggt gtgtactgtg
     1741 atgtgcttac cactggccag atcttcatcg atgatctgac ggataaagtt agtcgggcgg
     1801 gcttctgcct cactcatcgt ggattcctca aagcgtaaac aacgtataac ggcgtatgat
     1861 cttataagcg ggacaggctg acaactgtta gttttttaaa attgataatc taacaaggat
     1921 tatggggatg attgatgaaa aaaaaagcgg cagagaagat ctccaccgct tgtcattgtt
     1981 ggatgcgacg ctgccgtgtc gcatcaggca taaagcagat tactttttga tttcatacag
     2041 cggtgtttga cccgccacaa catggccctg agctttaatg agcaagccac tgaaatcgtc
     2101 gatattgctg caaaccaccg ggctaatcat cgagcgggcg ttagcgttca ggtaatccag
     2161 atccatttcc agaatcggtt gccctgcgct tacctgcgca ccctcttcca ccagacgttt
     2221 aaagccttta ccttccagcg ctacggtgtc gatacccata tggacgacga tctccgcgcc
     2281 tttttcggtt tccaggcaga acgcgtggtt ggtgttgaag attttcacga ttgtccctgc
     2341 ggctggtgat acgacgattt tatctgtcgg tttcaccgcc acaccgtcac ccaccgcttt
     2401 gctggcgaat gcttcgtcag gaacctgatc cagtgccacg acatcaccgg taatcggcga
     2461 taccagctcc gcgatagata ccgcgtttgg tacagcctgc ggttttgcta caggcgcggc
     2521 agttgccgga gttgcttcag ctgacgcagc ggctaccgga ccacgggcaa cgactttctt
     2581 catcgcatcg ccgatggatt ctgctttcgc gccaacaatc acctgaatag tctgtttgtt
     2641 cagtttcact acaccagaag cacccagacg tttacacatc gtatcgttga cgcgggcaga
     2701 gtcagccacg gtcaggcgca gacgggtaat acaggcatca atcgctttca ggttgtcagt
     2761 accgccaacc gcagcaatat agttggttgc cagttgagtc agaccttctt cagtattgct
     2821 gttggcttct tcagtaacga tctcgtcttc tttatcttca cgacccggcg ttttcaggtt
     2881 gaacatgcgg ataaccaaac tgaacaccac gaagtagata gcgaagaaga taacgcccat
     2941 caccagcagc atccagacgt tctggctggc ggcaggcagg ttatacatca acgcgtagtc
     3001 gatagcaccc gcagagaaag agaagccggc atggataccc agcagcgttg ccacaaacag
     3061 gctgataccg gtcagcagtg cgtgcaggag gtacagcagc ggcgcaagga acatgaacag
     3121 gaattccagc ggctcagtca caccggtcag gaacgcagta acagcaacag aaagcagcat
     3181 cccgccaacc atcggacgac gctctttcgg tgctgcgaag tacatcgcca gcgccgcacc
     3241 cggcagaccg aacatcatga tcgggaagaa gccggacatg aacatccccg cggtgccgtc
     3301 accagcgtag aaacggttga tgtcgccgtg gaaaaccgta cccgccgcgt tggtgaattc
     3361 accaatctgg aaccaggcga tggtgttcag cacctgatgc agaccggttg ggatcagcag
     3421 acggttgatg aaaccaaaga taccggaacc cagcgcgccc gcagaaacga tccactcgcc
     3481 gcctgcatgg atagcgtgct gtaccggcgg ccagacgtaa ccaaaaatgg ccgccagcac
     3541 cagacagaag aagccggtgg cgatcggcac aaagcgtttg ccgccgaaga agctcaggaa
     3601 gtccggcagt ttaatatcgg accaacggtt ataggctgcg ccaccaacca gaccggtaat
     3661 gatacccgcc agtacaccca tgttaatttc tgggttgatg gtcaccatcg ctttggttaa
     3721 cacaaagtaa cctaccgcac ccgccagcgc cgccgcacct gcgctgtctt tcgaccagct
     3781 ggatgccaca ccgatggcga agattaatgc gaggttatca aaaattgcac cgcccgcctg
     3841 ggcaataaac gcaacgttaa gtaaatctgg ctgaccgaat cgcagcaaca gcgccgccac
     3901 cggcagcacc gcgataggga gctgtaacgc cctaccgagt cgctggaaaa aacctaaaat
     3961 attcatctta ttccccctac gagaacccta tttggctcgt ttaaagccgt atttttattt
     4021 tgctgcaaat tttactgccg atgttctgta atcagattgt tagatcatct gctacagagt
     4081 gtgtgaaaat ttaattcgca tcgcaaatta aacgcgtgtc ttttgtgagt tttgtcacca
     4141 aatatcgtta tcatcactcc cttttactgg ctaaaccaga aaacttattt tatcattcaa
     4201 aaaatcaagt cggattgacg cctgtctgcg caaatccagg ttacgcttaa agatgtctaa
     4261 tccgccaacg gcttacattt tacttattga ggtgaataat gagactgatc cccctgacta
     4321 ccgctgaaca ggtcggcaaa tgggctgctc gccatatcgt caatcgtatc aatgcgttca
     4381 aaccaactgc cgatcgtccg tttgtactgg gcctgccgac tggcggcacg ccgatgacca
     4441 cctataaagc gttagtcgaa atgcataaag caggccaggt cagctttaag cacgttgtca
     4501 ccttcaacat ggacgaatat gtcggtctgc cgaaagagca tccggaaagc tactacagct
     4561 ttatgcaccg taatttcttc gatcacgttg atattccagc agaaaacatc aaccttctca
     4621 acggcaatgc cccggatatc gatgccgagt gccgccagta tgaagaaaaa atccgttctt
     4681 acggaaaaat tcatctgttt atgggcggtg taggtaacga cggtcatatt gcatttaacg
     4741 aaccggcgtc ttctctggct tctcgtactc gtatcaaaac cctgactcat gacactcgcg
     4801 tcgcgaactc tcgtttcttt gataacgatg tcaatcaggt gccaaaatat gccctgactg
     4861 tcggtgttgg tacactgctg gatgccgaag aagtgatgat tatggtgctg ggtagccaga
     4921 aagcactggc gctgcaggcc gccgttgaag gttgcgtgaa ccatatgtgg accatcagct
     4981 gtctgcaact gcatccgaaa gcgatcatgg tgtgcgatga accttccacc atggagctga
     5041 aagttaagac tttaagatat ttcaatgaat tagaagcaga aaatatcaaa ggtctgtaat
     5101 tgttatccct gccctggttc cttgctcagg gcaatatttt ttaaaatcgg ggggcagaat
     5161 gtatgcatta acccagggcc ggatctttac cggccacgaa tttcttgatg accacgcggt
     5221 tgttatcgct gatggcctga ttaaaagcgt ctgtccggta gcggaactgc cgccagagat
     5281 cgaacaacgt tcactgaacg gggccattct ctcccccggt tttatcgatg tgcagttaaa
     5341 cggctgcggc ggcgtacagt ttaacgacac cgctgaagcg gtcagcgtgg aaacgctgga
     5401 aatcatgcag aaagccaatg agaaatcagg ctgtactaac tatctgccga cgcttatcac
     5461 caccagcgat gagctgatga aacagggcgt gcgcgttatg cgtgagtacc tggcaaaaca
     5521 tccgaatcag gcgttaggtc tgcatctgga aggtccgtgg ctgaatctgg taaaaaaagg
     5581 cacccataat ccgaattttg tgcgtaagcc tgatgccgcg ctggtcgatt tcctgtgtga
     5641 aaacgccgac gtcattacca aagtgaccct ggcaccggaa atggttcctg cggaagtcat
     5701 cagcaaactg gcaaatgccg ggattgtggt ttctgccggt cactccaacg cgacgttgaa
     5761 agaagcaaaa gccggtttcc gcgcggggat tacctttgcc acccatctgt acaacgcgat
     5821 gccgtatatt accggtcgtg aaccgggcct ggcgggcgcg atcctcgacg aagctgacat
     5881 ttattgcggt attatcgctg atggcctgca tgttgattac gccaacattc gtaacgctaa
     5941 acgccttaaa ggcgacaaac tgtgtctggt taccgacgcc accgcgccag caggtgccaa
     6001 cattgaacag ttcatttttg cgggtaaaac aatatactac cgtaacggac tttgtgtgga
     6061 tgagaacggt acgttaagcg gttcatcctt aaccatgatt gaaggcgtgc gtaatctggt
     6121 cgaacattgc ggtatcgcac tggatgaagt gctgcgtatg gcgacgctct atccggcgcg
     6181 tgcgattgac gttgaaaaac gtctcggcac gctcgccgca ggtaaagtag ccaacctgac
     6241 cgcattcaca cctgatttta aaatcaccaa gaccatcgtt aacggtaacg aggtcgtaac
     6301 tcaataagag aaagtatgac accaggcgga caagctcaga taggtaatgt tgatctcgta
     6361 aaacagctta acagcgcggc ggtttatcgc ctgattgacc agtacgggcc aatctcgcgg
     6421 attcagattg ccgagcaaag ccagcttgcc cccgccagcg taaccaaaat tacgcgtcag
     6481 cttatcgaac gcgggctgat caaagaagtt gatcagcagg cctccaccgg gggccgccgc
     6541 gctatctcca tcgtcaccga aacccgcaat ttccacgcaa tcggcgtacg gcttggtcgt
     6601 catgacgcca ccatcactct gtttgatctc agcagcaaag tgctggcaga agaacattac
     6661 ccgctgccgg aacgtaccca gcagacgctg gaacatgccc tgctgaatgc cattgctcag
     6721 tttattgata gctaccagcg caagctgcgc gagctaatcg ccatttcggt gatcctgcca
     6781 gggcttgttg acccggacag cggcaaaatt cattacatgc cgcatattca ggtagaaaac
     6841 tgggggctgg tagaagcact ggaagaacgt tttaaagtga cctgtttcgt tggtcacgat
     6901 atccgtagtc tggcgctggc ggagcactac ttcggtgcaa gtcaggattg cgaagactcc
     6961 attctggtgc gtgtccatcg cggaaccggg gccgggatta tctctaacgg gcgcattttt
     7021 attggccgca acggtaacgt cggtgaaatt ggccatattc aggtcgaacc gctgggtgaa
     7081 cgctgccact gcggcaactt tggctgcctg gaaactatcg ccgccaacgc tgccattgaa
     7141 caacgggtgt tgaatctgtt aaagcagggc taccagagcc gtgtgccact ggacgactgc
     7201 accatcaaaa ctatctgcaa agccgcgaac aaaggcgata gcctggcctc ggaagtgatt
     7261 gagtatgtcg gtcgtcatct gggtaaaacc atcgccattg ctatcaactt atttaatccg
     7321 caaaaaattg ttattgctgg tgaaatcacc gaagccgata aagtgctgct ccctgctatt
     7381 gaaagctgca ttaataccca agcgctgaag gcgtttcgta ctaatctgcc ggtggtacgt
     7441 tctgagctgg atcaccgctc ggcaatcggc gcttttgcgc tggtaaaacg cgccatgctc
     7501 aacggtattt tgctccagca tttgctggaa aattaatgtg cttttatagt ggcgcttatt
     7561 gttgtcaata ttctgggtag tccatgacca ttaaaaatgt aatttgcgat atcgacggcg
     7621 tgctgatgca cgataacgtc gccgtaccgg gtgcagcgga atttttgcac gggattatgg
     7681 ataaaggcct gccgctggtg ttgctgacca actatccttc gcagactggg caagatctgg
     7741 cgaaccgctt tgccactgcc ggtgtcgatg tgccggatag cgtgttttat acctctgcga
     7801 tggcgaccgc cgatttcctg cgtcgctagg aaggcaagaa agcgtatgtg gtgggcgaag
     7861 gcgcactgat tcatgaactg tacaaagccg gtttcactat taccgatgtg aaccctgatt
     7921 tcgtgattgt tggcgaaaca cgttcctaca actgggacat gattcataaa gcagcctatt
     7981 tcgtcgctaa cggtgcacgc tttatcgcca ccaacccgga cacccacggg cgcggttttt
     8041 atcccgcttg tggcgcgttg tgtgcaggga ttgagaaaat ctccgggcgc aaaccgttct
     8101 atgttggtaa gcccagcccg tggatcatcc gcgcggcatt aaacaaaatg caggcgcatt
     8161 cggaagaaac agtgattgtc ggcgataacc tgcgtaccga tattctggcg ggcttccagg
     8221 ccggtctgga gacgattctg gtgctttctg gtgtttcatc gctcgacgat atcgacagta
     8281 tgccgttccg cccaagctgg atttacccgt cagtcgctga aatcgacgtt atctgaatac
     8341 cacccacgtc tgcggacgtg gttggctctg atgacaaacg ccaaactgcc tgatgcgcta
     8401 cgcttatcag gcctacgcag ctcctgcaat atattgaatt ttcatgcttt tgtaggccgg
     8461 ataaggcgtt cacgccgcat ccggcatgaa caaatcgcac tttatcagca atttggcccc
     8521 cgtaaaatat aaccattcaa taaaacccgc taaaaattat cgattcatca ataaatacat
     8581 cctcacacca gacccttttt caccaatcga caatcaccat tacgtttttt atttttgcac
     8641 tgccaaatag cttgcgcaca gtgctgcttt gcggcatttt tttaaacaag caaacacaac
     8701 aagcaacaaa taccaggtta acggagaagg ttatgtgttc aatttttggc gtattcgata
     8761 tcaaaacaga cgcagttgag ctgcgtaaga aagcactcga gctgtcacgc ctgatgcgtc
     8821 atcgtggccc ggactggtcc ggtatttatg ccagcgataa cgccattctc gctcacgaac
     8881 gtctgtcaat tgttgacgtt aacgcggggg cgcaacctct ctacaaccaa caaaaaaacc
     8941 acgtactggc ggtaaacggt gaaatctaca accaccaggc attgcgcgct gaatatggcg
     9001 atcgttatca gttccagaca gggtctgact gtgaagtgat cctcgcgctg tatcaggaaa
     9061 aagggccgga atttctcgac gacttgcagg gcatgtttgc ctttgccctg tacgacagcg
     9121 aaaaagatgc ctacctgatt ggtcgcgacc atctggggat catcccactg tatatggggt
     9181 atgacgaaca cggtcagctg tatgtggcct cagaaatgaa atcgctggtg ccagtttgcc
     9241 gcacgattaa agagttcccg gcggggagct atttgtggag tcaggacggc gaaatccgtt
     9301 cttattatca tcgcgactgg ttcgactttg atgcagtgaa agataacgtg accgacaaaa
     9361 acgagctgcg tcaggcactg gaagattccg ttaaaagcca tctgatgtct gatgtgcctt
     9421 acggtgtgct actttctggt ggtctggatt cctcaattat ttccgctatc accaagaaat
     9481 acgcagcccg tcgcgtggaa gatcaggaac gctctgaagc ctggtggccg cagttacact
     9541 cctttgctgt aggtctgccg ggttcaccgg atcttaaagc agcccaggaa gtggcaaacc
     9601 atctgggcac ggtgcatcac gaaattcact tcactgtaca ggaaggtctg gatgccatcc
     9661 gcgacgtgat ttaccacatc gaaacttatg atgtgaccac tattcgcgct tcaacaccga
     9721 tgtatttaat gtcgcgtaag atcaaggcga tgggcattaa aatggtgctg tccggtgaag
     9781 gttctgatga agtgtttggc ggttatcttt acttccataa agcgcccaac gttaaagaac
     9841 tgcatgaaga gacggtgcgt aaactgctgg ccttgcatat gtatgactgc gcgcgcgcca
     9901 acaaagcgat gtcagcctgg ggcgtggaag cacgcgttcc gttcctcgac aaaaaattcc
     9961 ttgatgtggc gatgcgcatt aacccgcagg ataaaatgtg cggtaacggc aaaatggaaa
    10021 aacacatcct gcgtgaatgt tttgagtcat acctgcccgc aagcgtggcc tggcggcaga
    10081 aagagcagtt ctccgatggc gtcggttaca gttggatcga caccctgaaa gaagtggcgg
    10141 cgcagcaggt ttctgatcag caactggaaa ctgcccgctt ccgcttcccg tacaacacgc
    10201 cgacctcaaa agaagcgtat ctgtaccgcg agatctttga agaactgttc ccacttccga
    10261 gtgccgctga gtgcgtgccg ggcggtcctt ccgtcgcgtg ttcttcagct aaagcgatcg
    10321 aatgggatga agcgttcaaa aaaatggacg atccgtctgg tcgtgcggtt ggcgttcacc
    10381 agtcggcgta taagtaagaa atgcggtact gccccggaga aatccggggc tgttttagtt
    10441 attgcgctgc ttgttgattc tgatatgcag taaggaaatg ttccgggttt gccacatcac
    10501 tgtttgacaa ctcatcactg taggccagta tttcatagat taatttccca ccactattgt
    10561 tgaattccga atttcatcct gtacgacaaa agaatggctg tagggtgggg aaaaacacaa
    10621 agtaaacaat aattgacgaa tatagcgcca cgctgttcgc aacctaacca aacagtcact
    10681 ttcgagcaat tttccttgaa aaagaggttg acgctgcaag gctctatacg cataatgcgc
    10741 cccgcaacgc cgataaggta tcgcgaaaaa aagatggcta cgtagctcag ttggttagag
    10801 cacatcactc ataatgatgg ggtcacaggt tcgaatcccg tcgtagccac catctttttt
    10861 gcgggagtgg cgaaattggt agacgcacca gatttaggtt ctggcgccgc aaggtgtgcg
    10921 agttcaagtc tcgcctcccg caccattcac cagaaagcgt tgtacggatg gggtatcgcc
    10981 a
//
LOCUS       DAZPAB010000126        10897 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZPAB010000126 DAZPAB010000000
VERSION     DAZPAB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10897
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.126"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(24..344)
                     /gene="chbB"
                     /locus_tag="ACHUPG_002863"
     CDS             complement(24..344)
                     /gene="chbB"
                     /locus_tag="ACHUPG_002863"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR0862396.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(643..981)
                     /gene="osmE"
                     /locus_tag="ACHUPG_002864"
     CDS             complement(643..981)
                     /gene="osmE"
                     /locus_tag="ACHUPG_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR0862397.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1183..2010
                     /gene="nadE"
                     /locus_tag="ACHUPG_002865"
     CDS             1183..2010
                     /gene="nadE"
                     /locus_tag="ACHUPG_002865"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR0862398.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            2240..3127
                     /gene="cho"
                     /locus_tag="ACHUPG_002866"
     CDS             2240..3127
                     /gene="cho"
                     /locus_tag="ACHUPG_002866"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR0862399.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3087..3662)
                     /gene="ves"
                     /locus_tag="ACHUPG_002867"
     CDS             complement(3087..3662)
                     /gene="ves"
                     /locus_tag="ACHUPG_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR0862400.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3865..4350)
                     /gene="spy"
                     /locus_tag="ACHUPG_002868"
     CDS             complement(3865..4350)
                     /gene="spy"
                     /locus_tag="ACHUPG_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR0862401.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4680..5648)
                     /gene="astE"
                     /locus_tag="ACHUPG_002869"
     CDS             complement(4680..5648)
                     /gene="astE"
                     /locus_tag="ACHUPG_002869"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR0862402.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5652..6973)
                     /gene="astB"
                     /locus_tag="ACHUPG_002870"
                     /pseudo
     CDS             complement(5652..6973)
                     /gene="astB"
                     /locus_tag="ACHUPG_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(6970..8448)
                     /gene="astD"
                     /locus_tag="ACHUPG_002871"
     CDS             complement(6970..8448)
                     /gene="astD"
                     /locus_tag="ACHUPG_002871"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFR0862403.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8445..9479)
                     /gene="astA"
                     /locus_tag="ACHUPG_002872"
     CDS             complement(8445..9479)
                     /gene="astA"
                     /locus_tag="ACHUPG_002872"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR0862404.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9476..10696)
                     /gene="astC"
                     /locus_tag="ACHUPG_002873"
                     /pseudo
     CDS             complement(9476..10696)
                     /gene="astC"
                     /locus_tag="ACHUPG_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
BASE COUNT         2645 a         2978 c         2692 g         2582 t
ORIGIN      
        1 ttgacgggaa aatttaaaat aaattaattt gctgcggctt ttttaatcgc tgcaaccgca
       61 gccttaagca cgcctaaacc atcgactttg ccataaagca gcgagtcaat tacttcaacc
      121 ggtttgttgg gtaacaaacg ctggatttcg ggcaacatat aagcaatctg cggccctaat
      181 aacacgacat cggcattctg acctttttca ccagccagtg tttccggaaa tgcttcaata
      241 atgaccggaa cttcatattt ttctgcctgt gcgcgcattt ttgataccag taaagagata
      301 gacatgcccg cagaacaaaa cagataaatg tgtttctttt ccataaaact gccctcatcg
      361 acgattatct gtcagccaga cactccgcaa gccttgacct gcttccatgc tctgggtaac
      421 ttgcgaaacc aacatgactt tttttgtggc tgaaaggagt atacggtata gacctgagca
      481 atagtatttc tttgacctta taaattgttt ctccttgacc tgtggttatg accctcttca
      541 catttcgggc aaataattcg cgaagataat taagaactca ggccaaaaaa aaccggcgca
      601 atggccggtt tccgttgtta ctcaagcttt cagacgaatt gattacttcg cagcctgtgg
      661 atcagtgtcg tattcagcac aggtctgata accggagttg atgacatgtc cggtatcatc
      721 taacgcgaca aagtaggttt ctgctttacc atcacgttga cccaggatgt aggtctggca
      781 agtaccgcga gcatggatca tgctcacttc agacgaaggt ttacccgcaa tttgtgcaac
      841 ctgcgcccgg ctcatgcctt ttttgacgtc tttcaccaca ggctgtacaa actggtcttt
      901 ggtacgatca taagctgtac aacccgccag catggttaat accgccgctg cactcagaat
      961 tcctgccata ttcttgttca tattccgtcc tcttgtttat cagcgtgtta gataagcctg
     1021 gaatacattg ggcgcttttt caagcccgtg aacgaaacgg ctccgctttc agaggattcc
     1081 tgtatgacgt tttaaccacc attcagcccg ctgtcgcttg tcgtttcagt agcaacgggt
     1141 tagctttaag gaagttttgt cttttctgtc tggaggggtt caatgacatt gcaacaacaa
     1201 ataataaagg cgctgggcgc aaaaccgcag attaatgctg aagaggaaat tcgtcgtagt
     1261 gtcgattttc tgaaaagcta cctgcaaact tatccgttca ttaaatcact ggtgctcggg
     1321 atcagcggcg gtcaggactc cacgcttgcc ggaaagctgt gccagatggc gattaatgag
     1381 ctgcgccagg aaactcgcaa cgaatcactg caatttattg ccgtacgcct gccctatggt
     1441 gttcaggctg acgaacaaga ttgccaggat gccattgcct ttattcaacc ggatcgcgta
     1501 ttaaccgtta atatcaaggg cgcggtattg gctagcgagc aggcattgcg ggaagcaggc
     1561 attgaactga gcgattttgt ccgtggcaat gaaaaagcgc gtgagcggat gaaagcacaa
     1621 tatagcattg cgggtatgac cagcggtgtc gtggtgggca ccgatcatgc agcagaagcc
     1681 attaccggat tcttcactaa atatggtgac ggcggtacgg atattaatcc gctgtatcgt
     1741 ctcaacaaac gccagggtaa acagttactg acggcattag gttgcccgga acacctttat
     1801 aagaaagccc caactgccga tcttgaagat gatcgccctt ctctgccaga tgaagtggca
     1861 ctcggcgtga cctatgacaa tatcgacgac tatctggaag ggaaaaacct acctgaacag
     1921 gtcgccagaa caatagagaa ctggtatctg aaaaccgaac ataaacgccg tccgccaatt
     1981 aacgttttcg atgatttctg gaaaaagtaa taatttgcac ctattggatt gtgcgaaaaa
     2041 gagtaatttg ttcacgccgg atacggcgtg aacgccttat tctacctaca aaactatgca
     2101 aattcaatac attgcaggag tcgaataggc ctgacaggcg tagcacgtca gacggtgtaa
     2161 cctttgtcat caacccgcct cttttttaat cgcctcccgc ctgttacact ggatagataa
     2221 ccagcattcg gagtcaacag tggtacggcg tttaacttct ccgcggctcg aatttgaagc
     2281 tgcggcaatt tatgaatatc ccgaacattt acattcattc cttaatgact tacccacccg
     2341 accaggggtg tatctgtttc atggcgaaag tgacaccatg ccgctctata tcggcaaaag
     2401 cgttaatatc cgcagtcggg tgctttctca tttacgcacc ccggatgaag ccgccatgct
     2461 acggcaatcc cggcgaatta gctggatctg caccgcaggc gaaatcggcg ctctgctcct
     2521 tgaagcgcga ttaatcaaag aacaacagcc gctgtttaat aaacggttgc gccgcaatcg
     2581 ccagctctgt gccctgcaat taaatgaaaa gcgcgtcgat gtggtgtatg ccaaagaggt
     2641 ggatttttca cgagccccca acctgtttgg cctgtttgcc aataggcgcg cagctttgca
     2701 agcattgcag agcatcgctg atgaacaaaa actttgttat ggcctgttgg gactggaacc
     2761 gttaagtcgc ggtcgtgcat gttttcgttc agcgctaaaa cgttgcgccg gagcatgctg
     2821 cggtaaagag agccatgagg aacatgcgct acgcttgcgc cagtctctgg agcgtttgcg
     2881 agtggtgtgt tggccttggc aaggtgcggt ggcactgaaa gaacagcacc cggaaatgac
     2941 tcaatatcat attattcaaa actggctgtg gctgggggcg gttaattcgc tggaagaagc
     3001 gacaacgtta attcggacac ccgccgggtt tgatcacgac ggttataaaa ttctttgtaa
     3061 gccgctgctt tccggtaact atgaaattac tgaacttgat ccggcgaatg accagcgagc
     3121 cagttgattt cactgaacaa cagtttacct tgcggttgta aaagccgcag ggtgtgcctg
     3181 ccatcgaacc agcacacgcc ctggtcggta gtgagcagtt tgtcgcccag ttgccacgcc
     3241 ccgttaatca caaacaccac cccgccgcgt gagccaaagg tagtaaacgt acgttcggca
     3301 attctgactt tcgccttaca gacatccagg cgcgtcataa tattgaaatc catcgacatc
     3361 tgccctgccg tcagtttcgc ttttaccacc tggtccgccg taaaggcaaa aggctgtaac
     3421 ggttttaagg tatggttaaa gcggtctgcg ctttcaagga gcatctcgcc gccttccagc
     3481 aacgtcacta tcctttccat gccgggaaac aaagaaaact ccccattcgc cgcgatggat
     3541 gcaatgctgg cacgccagta aaaatcacgt tttgccggtg ggaaagtgca aatttcgcgc
     3601 gtttcaccgg cagcgtttcg ccacagattc accgacattt tacgcatatc aaagtattcc
     3661 atactcgctc ccggcccgtt cttttcattg ttatcgttca ggtaaccgtg gatggttatc
     3721 ggcaactcgg gtgatttacg ttagtggtga tcaggctttt tttacaactg tttgagaaga
     3781 gaaaagaaaa ccgccgatcc tgtccaccgc attactgcaa ggtagtggac aagaccggcg
     3841 gtcttaagtt ttttggctga aagattattc agcagttgca ggcattttac cttttgccgc
     3901 tggacgttct gtcagacgct tctcaaaatt agcattaaat tgctttttct gttccggcgt
     3961 caggatgttg taaattttgt tctgggtttc catgtgcgcc agcatgttag ctttgcgctg
     4021 ttcttccatt tttgcgatct gcgcttcagc ttttgcttta tcgaaggtat cgctggcaat
     4081 gatgtcatgc attgcgcggc gttcttccag cggcggacgt ttcatctggt cacgctggcc
     4141 tttcatgatt tcgtggatct gctgtttctg cgcgtcggtc aggttcaggt ctttgaacat
     4201 catgtcctga tgcggaccga acttgccttt gtggtgcatc atcggcttcg cgtcagccgg
     4261 tgctgcggta gtggtgtctg cggcatgggc caggttagcc gcgccaagag ccagggtaga
     4321 ggcaacaaac agtgcagtta atttacgcat attctatatc cttcctttca gttatttatt
     4381 acggctttct taagtagcgt gccgtgttga cgagattaac tttactgact ttagcgtcaa
     4441 ttaatcagag caacggtaaa acaatgaaag tgtaaaaaac acttttgcgc caattatgga
     4501 gaaaaaaaga aaatttgatg gagagtgatg agagaatatt acaacacgat gattttgcag
     4561 agattatgaa gaactatacc ggatgactgg tgataaataa agcaaataac caggattaat
     4621 ctgtattaat ttataagaaa gcaacttaat acccgcagaa tgatttctgc gggtaagtat
     4681 tagcttattt tttcgagcat taatcccgcg cgtaatccca acgctaccaa cggattaggg
     4741 aataacacat actctacatc atgggttacg gtaaaacgtt cctctccgtc ctgcgccagc
     4801 aatgttcctt tctcaaacgg cataaaattc agcgtgtcac ttgccatatg catttcgaag
     4861 gacggcgagt ggcgagtaat ttgcgaaacc acccgataac ggagcggcgg tgttctcacg
     4921 ataccgacac tctcaccaga tagcagcgca gcaattgcgc tggcagttac tgcaaactgg
     4981 cgaagatcgt tttgcccaaa gggcaacgct ttgccaagtt ccaacgtaca ggccagcgcg
     5041 ccaaaatgtc tgacgctgaa atgggtaaac gtaccaccag gctcctgatg gaacaccagc
     5101 gcctccagcc ccgccgcacc cagccacgtc agaaatttct cgtcccaggg aatgtcgcgt
     5161 tgcggtaata caccgaactg cggatgcaag gagccacgaa ttgcggtatg tagatcaagg
     5221 tgccaacgca cagattcttt gccctggtca taaaaatctt ccaggcactg ttccagttcg
     5281 cgcgcccgac aggtttctcc gctttcagca aatagctgcc aacgaccgcc aaacattcgg
     5341 ttcatatcgc tatggcaata acgtttccct tgcttcagcg caggaggatt cccgaggatc
     5401 accagcaacc gccaacgtaa cgggatctcg ccgtgagata tcgcgccaag caacgcgtcc
     5461 agcatttcca caggtgccgt ctcattaccg tggatccccg cagaaatcac cagcgcgcct
     5521 tgcggtggcg ttaatggcgt cagttccagc acaccatcgc ccagccagcg ccagcgaacg
     5581 ccgttgattt ctcgctcggt gataaccggt tttttacccg ttaaggtcag agcaagaaaa
     5641 ttatccattg cccctccctc gcgctggaac ggataaaccg aaccgagatt cagtaattgg
     5701 ctcaatacat ccagtgcttc ccgcccttcg cgcagcaatt gcgggtcggc cagatcggca
     5761 gcagtaagac gatcgcggta gtaacgatcc acccagtcat tgagcgcatt aaacaaggta
     5821 tcgttcatca tcaccgccgg attcaccgcc cggcgttctt cttctgtcaa taccacccgc
     5881 aaccgcaggc acgccgggcc gccgccattc gccatgcttt cacgtaagtc aaagactttt
     5941 agttcgctaa tcgggttgtc agcggcaagg agttcattga gataacccca tactccggcg
     6001 tgttcccgac actcctgagg cagcaccaac atcatggaac catcatcgcg gctcaacagt
     6061 tggctgttaa acagataggt agacaccgca tccgacacgg aaacctgagt tgccggaact
     6121 tctatcgcca taaaaccgtt cacccgcgtg ccagtaactg tgactggcga gcgaacgcct
     6181 gttggtggca aaacagcacc tggcggttac tcacggcaat cacgtcatta tgaaaaacgc
     6241 tctggtcgat aacgtccggg ttttgctggg cgaaaatcac ctgttgggga ttcacctgat
     6301 taagccttgc caccgcctcg ctggcttcgc gagtctgtcg cgccggataa cgggaaggcc
     6361 gggtatcatt gccttcttct cgcccgtaga caaaaagtag catacccggt tcaccgtaat
     6421 gaccgccgag acgattgtgg tttgccgccc cctcatcacc gagcaacgct acctgcggca
     6481 acgccgaatg gacgctaaat ttctcttcgt ccttaaaaat cgcttttaac agcgattcag
     6541 tgacgggcgc ttccagcgaa cggtgaaatt tattgttcag gttggcaacg gtgagatgca
     6601 ctttgccatc cagcgtatcg gcagatggcg cgatcgttgc cgcattggct acccacattg
     6661 gcgaagcgga actgacgctg gaaagccagt gcggtgcctg gcgtgcaact ttttccagta
     6721 cctgctcatc gctaccactg aatcccaact gacgcagcac cggaataaac ggacgctcgt
     6781 gcggcggaat cacggcctgg gggaatcccg catcggcaag ggctttcatt ttcagtaagc
     6841 cctgttttgc cgccagtcgc gggttagaca cctgaaaacg gtgacgggta gaggcttcat
     6901 taccaaacga caggcccgcg taatgatgcg tcagccctac cagcccgtcg aaattaactt
     6961 cccaggcgtt catcgcacca cctcatcgga aaaatccagc ccggggttaa gcgtggcggg
     7021 caatgttaac gagtccgact ccaggctcgc cataggccat gcgcagtaat ctgcggcata
     7081 ccaggcgctg gggcgatggt taccggaagc accaatgccg ccgaatgggg cggtactggc
     7141 agcaccagta agcggtttgt tccagttaac aatccccgcc cgcgcctcca gcaacagttg
     7201 atcgaatttt tcccgctcgg gggaaaccag accgcaaaag agtccgaagc gagtgttgtt
     7261 cgccatccga atcgcctcat caaaagtatc ataacgccag acgcgcaaaa acggcccgaa
     7321 cacctcttca tccggcacac cgccaacgcc cgtcatttca atgatccctg gtgtcagcaa
     7381 cgatgtccct gcttgtaata agcgcggcgc aagcagggtt cgtccgccca tcgcttccag
     7441 ttgctgccag gcagtaacca cctgctgtgc ggcctgttca gaaatcagcc cgccaataaa
     7501 cggctgcggt tcgtcatccc agttgcccgg cgttaatcgc tggctgacgg caaccagact
     7561 agcaagaaac gcatcgccct gcgctccgct tttgagcaat aaacggcggg cgcaggtgca
     7621 gcgttgcccg gctgtgacaa acgccgactg aatggtcaga tggacagccg cgtcgatatc
     7681 cgccacctca tcgataatta gcggattatt accgcccatc tcaagggcga gaattttctc
     7741 cggctgaccg gagagctggc gatgcagctg gtagccggta ttggcgctac cggtaaacag
     7801 caaaccgtcg agatcctcca gcgcactcag cgcctgaccc gtttcacgcc cgccctgcac
     7861 caggttcagc acgcccggcg gcaagccagc ctgctgccat aaacgcatta ccgcttcgcc
     7921 actccacggt gtcagttcgc tgggtttaaa gataatggtg ttacctgcca gcaatgccgg
     7981 aacgatatgt ccgttcggca aatgaccagg gaaattatac ggcccaaaca ccgccagcac
     8041 gccgtgcggg cgatgtcgca ggctcgccgc accgtccggc atttcactac gctgctcgcc
     8101 ggtacgaacg tgatacgcct taattgatat cgcgatttta ttgatcatcg ccgtcacttc
     8161 ggttgccgct tcccagcgcg gcttacccgt ttctctggca ataatcgcgg ttaattcggc
     8221 tttattgctt tccagcaatc cggcaaagcg ttcgacaacg acctgacgtt cagccaatga
     8281 gagccgcgcc cagcgcggaa acgccgcacg ggctgcccga caagcctgtt cgacctgagc
     8341 ggcatcggca tcattgcctt gccataacac ctcgcccgat accggattac gcttcacacg
     8401 cgatgcgccc tggcccgtta tccagtcacc gttaatccat aaagtcatgc tgttttctcc
     8461 tctgcgcaca ggcgcaccag acgaacgcga tcccctgcgt ggcatttgag ggcatccagt
     8521 tgtgcggcgg ttaaaatcaa acgctcggtt gccggatcgg tacgcgccag caccacgcgg
     8581 aaatggtgat aattttcatt ggcgaccagg caggccggaa aatcgccctg cgcaggctgc
     8641 ccttctgcca cttccaccag ccgactttta cggatggcgc gcacgcggtc gatgtcacac
     8701 tcaagcgttg gcccgccgtc gaagatgtcg atatagttgc ggtaacgaaa accttctttc
     8761 tccagcaccg cgcgggcagg cgcggtttgc ggatgtacct gaccgatgac gtcctgggct
     8821 tcctgggata aaaagtgggt atagatcgga tgtttcggca tcagttctgc aataaatgcc
     8881 ttttgcccgg tgccgcagag aaaatcggcg cggctaaaat ccatcgaaaa gaagcgttta
     8941 ccgaggcttt gccagaacgg tgaatagccg tgttcgtcaa tcaccccgcg catttcagca
     9001 accactttgt cattaaactt gtcgcgaaaa gccgccataa acataaagcg cgatttcgac
     9061 agcaaatagc cgttgccctc tttgcgccag tccgggtcga gaaacagcgt gcacagctcg
     9121 ctgctgccgg tgtgatcgtt actaagaaac agcgtcggca atgcgttata gacattcagc
     9181 tcttttgagg cgtgaaccaa cgtgccgacg cgatagttgt accagggatc gttcagccca
     9241 accgccacct caatagcgca aatccccgcc acggtgcctg tctcgctatc ttccagcacg
     9301 aacacatagc cctgctcgct tttgggcagt tcaccttgcc aggttttgat tgccctttcg
     9361 atacgcgccg aaagcgtggc ttcattggcg ggaagcgacg tcaggccgcc gcccgttttg
     9421 ctggcaagct gcatcagcgc cgagacatct gagcgctcaa cgggacggat gaccatcatg
     9481 atgaacctcg gctaacaaag tgttcgcaag cagctgcaaa gcgatccagt ccggtcgtca
     9541 cctcttcttc gctgacattg agcgcaggcg caaaacgcac cacgttgcca cccgcaatca
     9601 gtaccatcac gcctgctttc gccgcttcca gagagatctg tttcgcttgc ccggcgtaat
     9661 cggcattcag tacacagcca atcaacaaac ctaagccgcg aacttcactg aacaaaccat
     9721 agcggtgatt aatagtatta agacgctcaa caaaccagtc gtgacgctgt ttaacgccat
     9781 taagcatctc tggtgtgttg atgagctcca gcactttgcc tgctaccgcc gaggccagcg
     9841 gattaccgcc ataggtggtg ccatgagtgc caacggtcat cacgcgggcg cactcttcgg
     9901 ttgccaacaa cgcaccgacc gggaaaccgc cgcccagcgc tttggcggtg gttaacagat
     9961 cgggcgtcac gccgtagtgc atataggcat acagctcacc agtgcgcccg acgccggttt
    10021 gtacctcatc aaaaatcagc aaggcattgt ggcggtcaca caattcacgc agaccttgta
    10081 aaaacgcgtt gctggctggc accacaccgc cttcgcccta gatgggctcg acaatcaccg
    10141 cacaggtggc gtcgtcaatc agcgcgctgg cagagttaat atcgttatat gcagcatgac
    10201 gaatatccgc cggcagtggc gcaaaatcct gtgaataggc tggctgccca cccgcactga
    10261 cagtaaacag cgtgcgacca tgaaacgcat ttttgaacgc cacgatgcca ctcttatggc
    10321 tgccgtagcg gtcgtgagcg aatttacgcg ccagttttag cgccgcttcg ttggcttccg
    10381 caccggagtt acaaaagaag acgcgatcgg caaacgtggc gtcgatcaat tttttcgcca
    10441 gtcgcagtac cggctcgttg gtgtaaccgt tgccggtatg ccagaactta ctcgcctgtt
    10501 cgttcagcgc ttcacgcagt tccggatgcg catggcccag cgcgttcacc gcaatgccac
    10561 ccgcgaagtc gatatactct ttcccctgct gatcccacaa gcgcgaacct tcgccacgta
    10621 ccggtataaa gggtgccgga gcgtaaacag gtatcatcca ttcatcaaag ttttcacgcg
    10681 taattggctg agacatagcg acctctacag taaataattc gttatttgta tgttaacaat
    10741 aagtaatgtt tgcgctgtaa atgtagattg cagggttcgt gccagccagt gataaaagtg
    10801 cataaacggc ggaggctaac tggaaatcaa ggagttataa ccaaaccata tgcatttaaa
    10861 gtgcatataa agtgaatacg tttgcgatgt gggtgaa
//
LOCUS       DAZPAB010000127        10658 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZPAB010000127 DAZPAB010000000
VERSION     DAZPAB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.127"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..1797)
                     /gene="selB"
                     /locus_tag="ACHUPG_002874"
     CDS             complement(<1..1797)
                     /gene="selB"
                     /locus_tag="ACHUPG_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR0862405.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRR"
     gene            complement(1794..3185)
                     /gene="selA"
                     /locus_tag="ACHUPG_002875"
     CDS             complement(1794..3185)
                     /gene="selA"
                     /locus_tag="ACHUPG_002875"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR0862406.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3283..3891)
                     /locus_tag="ACHUPG_002876"
     CDS             complement(3283..3891)
                     /locus_tag="ACHUPG_002876"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR0862407.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(3962..5047)
                     /locus_tag="ACHUPG_002877"
     CDS             complement(3962..5047)
                     /locus_tag="ACHUPG_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR0862408.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5050..5412)
                     /locus_tag="ACHUPG_002878"
     CDS             complement(5050..5412)
                     /locus_tag="ACHUPG_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR0862409.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            5949..7862
                     /gene="mtlA"
                     /locus_tag="ACHUPG_002879"
     CDS             5949..7862
                     /gene="mtlA"
                     /locus_tag="ACHUPG_002879"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR0862410.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            7957..9105
                     /gene="mtlD"
                     /locus_tag="ACHUPG_002880"
     CDS             7957..9105
                     /gene="mtlD"
                     /locus_tag="ACHUPG_002880"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR0862411.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9105..9692
                     /gene="mtlR"
                     /locus_tag="ACHUPG_002881"
     CDS             9105..9692
                     /gene="mtlR"
                     /locus_tag="ACHUPG_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR0862412.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(9704..9913)
                     /gene="yibT"
                     /locus_tag="ACHUPG_002882"
     CDS             complement(9704..9913)
                     /gene="yibT"
                     /locus_tag="ACHUPG_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR0862413.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            10197..10559
                     /locus_tag="ACHUPG_002883"
     CDS             10197..10559
                     /locus_tag="ACHUPG_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR0862414.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         2561 a         2880 c         2750 g         2467 t
ORIGIN      
        1 acgacgacgc gtaaagccaa tgcggtcaaa atattccaga atctgaattg ccagctttcg
       61 gcctacgcct aagcgatcgc ggaaatccgc cgcgcaggtt gaaccacact cctgatcgag
      121 atcgcggatc atattggcaa attcgacaat ccgatcgtta cggtaataac gatctttgac
      181 gatcgcggta attattcctt gctgcgccgc ctggcgtagc gtcaggcgca ttgcctgctc
      241 gtcggtaccc gtctcttttg ccagatcacg cacccaccac ggctcgtcac caaacagcgg
      301 ctctgctttt tgccagatgg cctgctgctc ttcgctgaag cccgctttgt gatccggcag
      361 atgcagccaa ccgtgatgac tgaggatgtc gccgctttcg cgcatctttt caatcagcaa
      421 cagtactagc gcttcatctt ccattggcaa cgccatacgt cgcagacgtt cgcgcccagg
      481 cccaggttca tcgcgatgtt gctcatgata agtcgctaat gtgtcgagaa ttttccgttg
      541 ccagcgagcg gcaaccggcg cattcaacaa gctataacca gcctgaatat agccaggctg
      601 ttgcagcagt tcgcgcatcc cttcgccgtt gagctggcgc gcccaagcga aatcaggaag
      661 attaaccgcg ccgcgttgca ggtgaacggc taacgcttcg ctatcattct gtgccctggc
      721 gagggaagcc agccattgca gatactctgg tttacgttta ccgcgacgcg gcgggttaag
      781 catcacgacg cgcgctccgg ccagcgtgtt gcgggcagag atatcgcgca ataccaggcg
      841 gtcgttatct gccagccata acggggtgtc gaagaccagt tcagcaaggt tatcttccag
      901 cagtgaaacg cgtcccgtga cgtggctggc ggcgtggtga atatgcagcg gctgccactg
      961 ggtcagcggt gcatgggttt gaagctcgac aatcacccgt gtgaacggct ctggcggcac
     1021 atcggcaagc agccagtcgc cacggttaat ctgctctttt tccgcatcac ccgcgatgtc
     1081 aagcgcgata cgctgcccgg cattggcggt ttctgttggc tggttttgcg catgcagcgc
     1141 acgtacacgc atcggtttat ttacaccagt cagccagagt gaatcgccta ccttcacttc
     1201 cccgcttaac gccgtaccgg tgacgaccag cccggcacct tttacggtaa atgcgcggtc
     1261 aatcgcgagg cggaaactat gttggctggc gtgctcgcgt tccggcaact gaagcagatg
     1321 ctcgcgcagg gcatcaattc cccgaccttc ggttgctgcg gtgataaaca gttttgcctc
     1381 agcaaaaccg tattcccgca gaacctcctt tacctggcgt tcaacctcat caacacgcgc
     1441 ttcgtccacg cgatcggctt tggtcagcgc cactgtcagc atcgggttac cggtcagctg
     1501 caaaatcgcc agatgctcac gggtctgtgc catcacgccg tcatcgcacg ccaccaccaa
     1561 cagcgcgtga tcgataccac caacgcccgc cagcatgttg gaaagaaact tttcatgacc
     1621 gggaacgtcg ataaaaccag gcacgcgacc atccggctgc ggccagtagg catagccgag
     1681 atcgatggtc atgccgcgct ttttttcttc cggcagacgg tcagcattta cgccagtaat
     1741 cgcctgcaat aaggttgttt tgccgtggtc aacgtgtccg gcagtcgcaa taatcatttc
     1801 aacaacatct ccaaaaaccg ttgctcatct tcaaggcagc gtaaatccag ccacaatcgt
     1861 ccgtcataaa tacgaccaat caccggcact ggcaattcac gccagcgggc ggctaatgac
     1921 tcaaggtggc taccgcgtcc atcatggggt gtaaacgtta atgccgcgct cggcaggcga
     1981 tcaaccggca gcgaaccact gccaatctgc gaaagacatg gcataacctg taccgcaaac
     2041 tccgcgccgt aatgtgcgac aaggggagcc tgtaaacgtt gtgcctggat ttgaatgacc
     2101 tctgcgctgc gggtaagcag gcgcagggtc ggtaattttt cactcagagc ttcagggtgt
     2161 aaataaagac gcaacgtggc ttccagcgcc gcgagggtca ttttatccgc gcgtaatgca
     2221 cgcttcagcg ggtggctttg caggcgggcg atcatctctt ttttaccaac gataatcccg
     2281 gcctgcggcc cgcctaacaa cttgtcgccg gagaagctca ccagactgac gcccgccgca
     2341 atcaactcct gcggcattgg ctctttcggc aaaccgtact ggctaagatc gaccagcgag
     2401 ccactgccta aatcagtcac cactggaaca tccagctctt tgccgagcgc caccagttca
     2461 gcttcgtcta tcgctttggt gaacccctga atgctgtagt tactggtatg tactttcatc
     2521 aacagtgcgg tattttcatt caccgcctga cgataatcat tcgcgtgcgt gcggttggtg
     2581 gtccctactt cgtgtagggt gcagcctgcc tgacgcataa catcgggaat acgaaacgcg
     2641 ccgccaatct ccaccagttc gccgcgagat accaccacct ctttcccgct ggcagtggcc
     2701 gccaacatca ataacaccgc cgccgcattg ttattgacga tacaggcatc ttccgccccc
     2761 gtaatacggc acagcagctg cgccagcgcc cgatcgcgat gtccgcgtcc ggcgtcgtcc
     2821 agatcgtact cgagggtcac tggcgaacgc atagcctgcg caacggcttc caccgcggct
     2881 tccgcctgta aagctcgccc aaggttggta tgcagcacgg ttcccgtcag gttgatcacc
     2941 ggacgcagcg cgctctgcgc ctctttcgtc aaccgggcat cgacttcttg cgcccagttt
     3001 tcacaccacg caggcagcgt ctggctgcca cgaatcactt ctcgcgcttc gtcgagcatc
     3061 tgacgcaaca attccaccac gcgggtgtga ccataagtat cacgcaaaga aaggaaggag
     3121 ctatcgcgca ataagcgatc aatagccgga agttgactat agagggaacg cgtttcggtt
     3181 gtcataggaa acctggctga tcaaggccct ctcacacgga gaagggcgtt taacataacc
     3241 acggattgta acgtgagatg ggtcaggagg acatatcgcg catcaagcct ttggcggttc
     3301 ggtgcgagca aaactttcgc ggctaaacag gttttcgacc agtttgacta agtgtgggcg
     3361 gtcgacacac caacctggcg caacgcggcg gaaattgaga tatccgacag cacaggcaat
     3421 ggcgatagtc gccagattga ccgtatcggt tttgagtgtg ccatcgacca gatatccttc
     3481 cagcacatcc agactgcggt tgattttctc ccgctggcgt aacaattcat cttcagactg
     3541 ctgcgccgcc ggacgcgcct gttcacgcac cgataccagc ccggcatcca taatgccatc
     3601 cgccagcgcc tcaattttgc gcacccgcaa cgactccagc ggatcgcgcg gcaacatcgc
     3661 cggagcgaca ttcattaact caatatattc agcgatgatc ggcgaatcaa accagcattc
     3721 gccctcttcg gtcaccagca ccggcacttt ccctaacggg ttaaattgcg ccacgccgtt
     3781 atccgcgtta tagggcagtt cattaataaa ttcgaaagtt atgccctttt ctaacaacag
     3841 aataaaaagt ttgcgtacaa acgggctggt gtagctaccg acgagtttca tgccgagtcc
     3901 tttgtgcgag gaaaaatatc agtatggctc aggctcacgc aaaacacagg tgatataagt
     3961 ctcaatgatc caaataaaga taatgcatcc aactggtcat tcttagcagc actttccgca
     4021 tcaccgaaac atggctgaaa tggtcggagt aaaccgccac ctgggcgtag atgccgtcgg
     4081 gtaaggcatc gatatcatcg ttagggtcca gttcaatggt tcccagcaca ccgtccgtgc
     4141 cgggaacgac cgttaatgat tgcaataccc cctgcgcctg ataagaaccg cctggcacga
     4201 caggtaaaat actagtcaat ttgccgtgga acacctgccc aggtagcgcg ttaaacacca
     4261 cttccgcatc atcgccaggt ttcagacgta acagcgagtt ttgccgaaat tgggcgacaa
     4321 tttgccgttt ttgctcgggg atgaagacca tcaccggacg cagcggcaag gcagctgcgt
     4381 atgtacctgg gcggatcagt acctgagtga cgtagccatt gcttggtgcg cgaatgacag
     4441 tctgctcaag attatatttt gcttcagtaa gttgcgctct taagctcaca atctgcgatt
     4501 gctcgccgtt gaccatactg tcgagctgac tctggatctg cgcctgctcc gccaccgagc
     4561 ctttcactaa cgcatcctgc gcgaggaaat tttgccgcgc atcgtcgatg tcacgttccg
     4621 agaacggatt caccgccgcc tggctgcctt tcaggtaacg ttgataattt ttaaacagac
     4681 ggtcgcgctc cgctgaaacc tgggtggtgt tggcctgcgc ttcagtgagc tgcgcacgca
     4741 gcgtctttat attatgcgtc gccgtcatca ggtcagcctg aagtctgtca actcgcgcct
     4801 ggtaacgaac cgggtcgagc ttaaaaagca cctcgccctt ttgaataagc tgattattct
     4861 tgtcagtgac ttcagtaaca attcccgtca cctgtggcgt gatagggatc gctatcactg
     4921 ccttttgcgc ggtaaaagtg taagggtggt tgtagttcat caacaaaatc aaaccactca
     4981 ccagaaacac gcctcccagc gccgccgtcg ccagcgccac gtaagttaaa acaatcaata
     5041 gatccataat tagatctccg ggaacgtgga atagtcagtc tttttctcgg cggcgagttg
     5101 atgctccagc cgggaaattc ggtcagaaag tgcggcgatt tccggttcag tcgctttctc
     5161 ctgcgacgca acatgtgact gcatacccca gccacgctcc ggttgataga gcgtcgccca
     5221 gatccacaga aacggccaga taacatgcag agtaaacagg ctcacccagc ccgccgtatg
     5281 aatagcgtcg gcatgggggt gattgcgctt tttggcaatc agataaggga tgtcatgtat
     5341 cgcgatgatt ccataaaaaa tcaccagaaa gacgaagatc agcactccca acgcgaaata
     5401 gtttagaaac atatctgcct cggattcacg tttatcagtg ttgttttttg gctggcagcc
     5461 agaagggagt caggctgata ttttgacaat aatccgggtt cgcgattctc gccataacac
     5521 caaagaataa tttttagagg tgatgagttg cttagttaca taacgattgt atgacgaagg
     5581 cataacatgc tgtagatcac atcaggtgaa cgccgtaaga aaatatcttg tgattcagat
     5641 cacaaagatt caacaaacca tcaaaacaaa aatgtgacat tactcacatt taaatgccat
     5701 ttttagcgaa aatcaccgcc ttgtttcttt tttatacagg cgttttgtga tgagcgtcac
     5761 gtcaattacc tctctacccc ctatatttat gtgactgata tcacacaaaa gcccgtcgac
     5821 tggacagtta accgattcag tgccagattt cgcagtatct acaaggtccg gctacctctg
     5881 ccgccacatt aacaaaaaac ctcgggcttc cagcctgcgc gacagcaaac ataagaaggg
     5941 gtgtttttat gtcatccgat attaagatca aagtgcaaag ctttggtcgt ttcctcagca
     6001 acatggtgat gccaaatatc ggcgcgttta tcgcgtgggg tatcatcacc gcgttattta
     6061 ttccaacagg gtggttaccg aacgagacgc tggcgaagct ggtcgggccg atgatcactt
     6121 atctcctacc gctgctgatc ggttataccg gtggtaagct ggtaggcggc aaacgtggtg
     6181 gcgtagtcgg tgccattacc accatgggcg ttatcgtcgg cgcagacatg ccgatgttcc
     6241 tcggttctat gattgcaggt ccgctgggcg gctggtgcat taagcacttc gaccgctggg
     6301 tagacggtaa gatcaaatcc ggttttgaga tgctggtgaa taacttctcc gcaggcatca
     6361 tcgggatgat cctcgctatt ctggcattcc tcggcattgg cccgattgtt gaagccctgt
     6421 ccaaaatgct ggctgcgggc gttaacttca tggttgtcca tgacatgctg ccgctggcgt
     6481 ctatctttgt tgaaccggcg aaaatcctgt tcctcaacaa cgccattaac cacggtatct
     6541 tctcgccgct gggtattcag cagtcccatg aactgggtaa atcaatcttc ttcctgattg
     6601 aagctaaccc aggtccaggt atgggcgtgc tgctggcgta catgttcttt ggtcgtggta
     6661 gcgctaaaca gtctgcgggc ggtgcggcaa tcatccactt cctgggtggt atccacgaaa
     6721 tctacttccc gtatgtgctg atgaatccgc gtctgatcct cgcagtcatc ctcggcggta
     6781 tgactggcgt gttcacgctg actatcctgg gcggtggtct ggtttctccg gcatctccgg
     6841 gttctatcct tgctgtactg gcgatgacac caaaaggtgc ttatttcgct aacatcgcgg
     6901 gtgtgtgtgc ggcgatggct gtctccttcg ttgtctctgc tattttgctg aaaaccagca
     6961 aagtgaaaga agaagatgat attgaagcag caactcgtcg tatgcaggac atgaaagctg
     7021 agtctaaagg cacatctccg ctatctgcgg gcgatgtgac taacgacctg agccacgtac
     7081 gtaaaatcat cgttgcctgt gacgccggta tgggttcaag tgcgatgggc gcaggcgtgc
     7141 tgcgtaagaa aattcaggat gcaggtctgt cgcagatttc ggtcactaac agcgcgatca
     7201 acaacctgcc gccagatgtg gacctcgtca tcactcaccg tgacctgacc gaacgcgcta
     7261 tgcgccaggt tccgcaggca cagcatattt cgctgaccaa cttcctcgac agcggcctgt
     7321 acaccagcct aaccgaacgt ctcgttgctg cccagcgcca tactgaaaac gaagtgaaag
     7381 tgaaagacag tctgaaagac agctttgacg attccagtgc taacctgttc aaactgggcg
     7441 cggagaacat cttcctcggt cgcaaagcag caaccaaaga agaagcgatt cgttttgctg
     7501 gcgagcagct ggtgaaaggc ggttacgttg agccggaata cgttcaggcg atgctggatc
     7561 gtgaaaaact gaccccgact tatctgggtg agtctatcgc ggtgccacac ggtacggttg
     7621 aagcgaaaga tcgcgtactg aaaacgggcg tggtgttctg ccagtacccg gaaggcgtgc
     7681 gcttcggtga agaagaagat gacattgccc gtctggtgat tggtattgca gcccgtaaca
     7741 acgagcacat tcaggttatc accagcctga ccaatgcact ggatgatgag tccgtcatcg
     7801 agcgtctggc acacaccacc agcgtggatg aagtgctgga actgctggca ggtcgtaagt
     7861 aatccaatcc taccctctcc tcacggagag ggtttgggtg agggaaaagc ctcaccccag
     7921 ccctctcggg taaaaacatt gatgaaggtt aatactatga aagcattaca ttttggcgca
     7981 ggtaatatcg gtcgtggctt tatcggtaaa ctgctggcag acgcgggtat ccaactgacg
     8041 tttgccgatg tcaatcaggt ggtacttgat gccctgaatg cccgtcatag ctatcaggtt
     8101 catgtggtcg gtgaaaccga gcaggtagat accgtttccg gcgtcaatgc tgtcagcagc
     8161 attggtgatg atgtcgttga tctgattgct caggtcgatt tagtcactac cgccgttggc
     8221 ccggttgtgc tggaacgtat tgctccggct atcgccaaag ggctggtgaa acgcaaagaa
     8281 caaggtaatg aatccccgct gaacatcatc gcctgtgaaa acatggtacg cggcaccacg
     8341 cagctgaaag gtcatgtgat gaacgccctg ccggaagacg ccaaagcgtg ggtagaagag
     8401 cacgttggct ttgtcgattc cgccgttgac cgcatcgtac cgccttcggc ttcggcaact
     8461 aacgatccgc tggaagtgac ggtagaaact ttcagcgaat ggattgtcga taaaacgcag
     8521 ttcaaaggcg cactgccgaa catcccaggc atggagttaa ccgacaacct gatggcattt
     8581 gtcgaacgta aactcttcac cctgaacacg ggtcatgcta taaccgcgta cctcggaaaa
     8641 ctggccggtc atcagaccat tcgtgacgct attctcgacg agaaaatccg cgcggtggta
     8701 aaaggtgcga tggaagaaag tggtgcggta ctgatcaagc gctacggctt tgacgcagac
     8761 aaacatgcgg cgtacatcca gaaaatcctc ggtcgttttg agaacccgta tctgaaagat
     8821 gatgtagagc gcgtaggccg tcagccgctg cgtaaactga gtgctggcga ccgtctgatc
     8881 aagccactgc tcggtacgct ggaatacagc cttccgcaca agaatctgat tcaggggatt
     8941 gctggtgcaa tgcacttccg cagtgaagat gatccacagg ctcaggaact ggcagcactg
     9001 atcgctgaca aaggtccgca ggcggcgctg gcacagattt ccggtcttga tgccaacagc
     9061 gaggttgtat ccgaggcggt aaccgcttat aaagcaatgc aataatggtg gaccaggcgc
     9121 aggacaccct gcgcccgaat aacagattgt cagatatgca ggcaacaatg gaacaaaccc
     9181 aggccgttga aaaccgtgtg cttgagcgtc tgaatgctgg caaaaccgtg cgaagctttc
     9241 tgatcaccgc cgtcgagctt ttgaccgagg cggtaaatct tctggtgctt caggtattcc
     9301 gcaaagacga ttacgcggtg aagtatgctg tagaaccgtt actcgacggc gacggtccgc
     9361 tgggcgatct ttctgtgcgt ttaaaactca tttacgggtt gggcgtcatt aaccgccagg
     9421 aatacgaaga tgcggaactg ctgatggcat tgcgtgaaga gctaaatcac gacggcaacg
     9481 agtacgcctt taccgacgac gaaatccttg gaccctttgg tgaactgcac tgcgtggcgg
     9541 cgttaccacc gccgccacag tttgaaccag cagactccag tttgtatgca atgcaaattc
     9601 agcgctatca acaggctgtg cgatcaacaa tggtcctttc actgactgag ctgatttcca
     9661 aaatcagctt aaaaaaagcc tttcaaaagt aagcaacgtc tgcttactgc ccctctacct
     9721 gcttcggccg ataaagccga cgataatact ccagacgttg tagatataac ggcacgcttt
     9781 catcgggtat tccggacgga atcgcgttac ggggaggaag ttttttcaga tactcccgga
     9841 acgcctggct tgatgccatg aaatctacgg ctttatcgat aagaagcgga acgttttcac
     9901 ctaatttgcc catgatgata tctccgtatt accccgcgcc gggaatgcgc ggccgccaat
     9961 tttagtttag gttcccggta aagatgacat taaggaaaac gtgctgaatc ctcaataatc
    10021 atccgctgct gtttattatc tttttctata gatttcttaa ttaatcaacg aattgcgtag
    10081 tttttgagca tgttaatgat tacgttgcag aactattaca atgtcatcag ttgtgaacaa
    10141 agcacctggt cgcgcatact aggggctata aatttatctt tatcagaagc catcacatga
    10201 aagaagtcga aaaaaacgaa atcaagcgtc tcagcgatcg cctggacgcc atccgccacc
    10261 agcaggccga tctgtcgctg gttgaagccg cagacaaata tgccgagctg gaaaaagaga
    10321 aagccacgct ggaagcagaa attgctcgcc tgcgtgaggt tcatagccaa aaactgagta
    10381 aagaagcaca gaagctgatg aagatgccgt tccagcgcgc aattaccaaa aaagagcagg
    10441 ctgatatggg caagctgaag aaaagtgttc gcggactggt ggttgtgcac ccaatgaccg
    10501 cgctggggcg cgaaatgggc ctggaagaga tgactggatt ttctaagact acgttttaag
    10561 cccctcagac gactggttcg atcgtatcaa cccgcatctg gcttgcctta gctggtcaat
    10621 gcggggactc tcctgtctac tcgcctaatt accaaaca
//
LOCUS       DAZPAB010000128        10636 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZPAB010000128 DAZPAB010000000
VERSION     DAZPAB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.128"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            3..827
                     /locus_tag="ACHUPG_002884"
     CDS             3..827
                     /locus_tag="ACHUPG_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFR0862415.1"
                     /translation="MPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIYLL
                     PLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKITS
                     ALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYAGT
                     TIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGNDP
                     IIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK"
     gene            complement(968..1741)
                     /gene="uxuR"
                     /locus_tag="ACHUPG_002885"
     CDS             complement(968..1741)
                     /gene="uxuR"
                     /locus_tag="ACHUPG_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR0862416.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1956..3416)
                     /locus_tag="ACHUPG_002886"
     CDS             complement(1956..3416)
                     /locus_tag="ACHUPG_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR0862417.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3497..4681)
                     /gene="uxuA"
                     /locus_tag="ACHUPG_002887"
     CDS             complement(3497..4681)
                     /gene="uxuA"
                     /locus_tag="ACHUPG_002887"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR0862418.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            5021..6364
                     /gene="gntP"
                     /locus_tag="ACHUPG_002888"
     CDS             5021..6364
                     /gene="gntP"
                     /locus_tag="ACHUPG_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR0862419.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6615..7517)
                     /gene="fimH"
                     /locus_tag="ACHUPG_002889"
     CDS             complement(6615..7517)
                     /gene="fimH"
                     /locus_tag="ACHUPG_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR0862420.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7537..7980)
                     /locus_tag="ACHUPG_002890"
     CDS             complement(7537..7980)
                     /locus_tag="ACHUPG_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0862421.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(8053..8583)
                     /gene="fimF"
                     /locus_tag="ACHUPG_002891"
     CDS             complement(8053..8583)
                     /gene="fimF"
                     /locus_tag="ACHUPG_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFR0862422.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8593..>10636)
                     /gene="fimD"
                     /locus_tag="ACHUPG_002892"
     CDS             complement(8593..>10636)
                     /gene="fimD"
                     /locus_tag="ACHUPG_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR0862423.1"
                     /translation="RIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNK
                     WQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV
                     IHGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIF
                     TVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQL
                     ADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNI
                     QLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLT
                     VTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGR
                     DQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLS
                     YSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHAN
                     GVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNT
                     LADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQS
                     SGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2536 a         2764 c         2635 g         2701 t
ORIGIN      
        1 ctatgccatt aagcaatgcc gctaataacc aattcaatac aaataatcac ttccttcatc
       61 atccaaaagt cgacagtgag ttaacacgtc gatatgaata tgcacgcctg gatacggaaa
      121 atatctatct tctgcctctt gctcgtggca ataaccataa ttacgatgga aaaagcgttg
      181 tcgaaattag aaaacttaat attggcaaac agtcctggcc atttaattat gtgacagata
      241 catgcagaga attcaatggt attacaacaa caggacgtat gctctatcga aacttgaaaa
      301 tcacctcagc tttagacgaa atatatggtg gtatatgtaa aaaggcgcat gctgctacag
      361 agttagcaga aggtttacgg ctaaatttat ttatgaagag ccctttcgat cctgttgagg
      421 actatacagt tcatgaaata acattaggcc ctggttgcaa tgttcctggt tatgccggaa
      481 ccacgatagg atatatttca acccttccag cttctcaggc aaaaagatgg accaatgagc
      541 agccaagaat tgatatctat attgatcaga taataactgt ctcaggggta gcaaattctt
      601 ccggattcgc ccttgccgca ttacttaatg ctaatattga gatgggaaat gacccaatca
      661 ttggtataga agcctatcct ggtaccgcgg agatccattc aaaaatggga tataacgtta
      721 tccccggtga tgaagatgcg ccactcaaac gcatgactct gcaaccgtca tctcttccag
      781 aactgtttga attaaaaaat ggtgaatgga attatattgg taaatagcca ctgagaagaa
      841 tgagtactaa tattcaggtt atacagatat ttaaacaaaa ccctgcaatt tacaaagtaa
      901 tactcattgc gacttcttaa tttaagaaag cgcaaggaac gtttaccctt gcgcttatta
      961 taataagtca ggcgtcgact ttatccagcg gccatgaatc aaagagatag ccgtcaaaat
     1021 aaatatcgtc aacgttcgag aattccagca gacgttgctt aacgttttcc agatgctgcc
     1081 acattgccag cttcgccgct cgggcatctt ttttgattaa cgcggcgagg atctgtttgt
     1141 gatcgcccaa ccactctttg cgatacaggc tgtcatccag atggctgtgc aactggatcc
     1201 acattggatt gttttcccgc cactgccagg actgacggaa taactccacc agcatgctgt
     1261 tatgcgttgc ttcagcaatg gcgagatgga actgcatgtc accgctttcg ctgctacccg
     1321 gcgcgctgga agccagttca cgctcttcca gttgcaatgc ctgacgcatt ttgacgatat
     1381 cttcgcgggt agcctgcagt gcggcaaact cggcgatgtt actttccagt aactgacgcg
     1441 cctgtaacag ctcaaaagga cccgcatcat tgcagacgtt ggcatccgga ctgtctgtgt
     1501 tgtggctgcc tgagctatca agcacataga taccggcacc ccggcgcact tccaccagcc
     1561 ctttgatctc cagcatgatc agcgcttcac gtaccaccgt ccgcgtgaca tcaagcattt
     1621 ctgcaatttc acgctccggc ggcagccgtt cgccaggatt gtacggcgtc tttatgatca
     1681 gatcgtggat catcgccccg acttcctggt aaggtctttg cgcagaggtg acagatttca
     1741 tggtgttgtg tccgttaggt gcggttaatc aaatttttgc cggatgcggc gtgaacgcct
     1801 tatccggcct acaacgggca attttacggc ccccgttaat ctcaaactat accattagca
     1861 ctggaagttg gcacgtacag gtgggcaaaa aaacgaaaac tggtcaacca aatatcagtt
     1921 tgattgacca gtaagtctgt agggtaatca gttagttaca gcgcagccac acactcgcgc
     1981 gcaccgcgtt cgcacagctg ctgatatgcc gacgtcaccg cgccaacaaa gtcggcattc
     2041 tgcggcagat catcggcaaa aataccgctc aggcccagca gcgctttcac gcggtctgcg
     2101 ccctgatact gcgcgttaat cttctggaac tcggccagca tcgggtcgac cacgtcaatg
     2161 gcattaccct gctcatccac gccctgggtg taacccatcc agccagccac gcccagcgcc
     2221 aggtgacgcc agctgccgcc gttttgcagg tgcagacgca ccgggtccag tagacgctgc
     2281 ggtaacttct ggctgccgtc catcgcaatc tgccaggtac ggtgacgcag agacgggttg
     2341 ctgaaacgct cgatcagcag cgtcgcatag gcgttcaggt ccgtaccttc cggcatcgac
     2401 agcgttggcg cttgttcctg catcatcagg gcaaaggccg ctttgcgata atccgggtta
     2461 gtcatggtgt cggcaatggt ttcatagccg ccgaggtaac cgaggtacgc caggaaagag
     2521 tggctgccgt tcagcatacg cagcttcatc atttcgaacg gcacaacgtc tgcaacgaac
     2581 tgtgtgccca ctttatccca gtccgggcga ccattaacga agttatcttc aatcacccac
     2641 tgacggaacg gttcgcaggc aatggcgcac gggtcgtaaa cacccagctg gtcagcaatt
     2701 tcctgtaagg tttctggcgt cgccgccgga acgatgcggt caaccatggt gcacgggaag
     2761 gtgacgttct cttcaatcca tgccgctagc tgcgggtcac gcgcctgagc cagccccagt
     2821 accgcgacct tcgccacatg gccgttttca cgcacgttat cgcaggacat caccgtaaat
     2881 gctttcagcc ctttttcacg acgcagacgc aaggcttcga cgatgtaacc aatcgcggac
     2941 ttcggcgcag tcgggttttc cagatcgtgc ttgatcagcg ggttattgag atccagttga
     3001 ccgcttgccg catcagcgca gtagcctttt tccgtgaccg ttagagagac aatcgccgtt
     3061 tgcggacgcg ccatcgcgtt gagaatacct tcgcagccgt cgatttccgg atgcagcgct
     3121 tctttcatcg aaccgataat tttcagctcg gtgctttctg cgcctttttc cgctacggtg
     3181 tacagcagtt gctgtttttt caggttttcg atcagcatgc ggtcgttgcc tggcatcagg
     3241 ttaacttcgc agatgcccca gtcgctgtcg gtgctttcca gcagatgatg ggtatacagc
     3301 gcctggtgcg cgcggtgaaa cgccccgcaa ccgagatgca caatgcgtga ttccagacga
     3361 gaatgatccc atgacgggcg ggcaaccggc agattgctgt caacaatagt agtcatcgta
     3421 aactccagaa tccgtcttca gagagtgacg gcaaggaaga aggaaccggc acggcgcgca
     3481 gccatgccgg tgggtattaa cggataaaga aagcgcgctg gatcgccagt tcgacaccgc
     3541 gaacttcggc caggcctttc agacgaccaa ttgcggagta accagggttg gttttcttct
     3601 tcaggtcgtc cagcatctga tgaccgtggt ccggacgcat cgggatcagg tcttctttgc
     3661 cttccgcttt acgacggtgt tcttcttcaa caatcgcttt caccactttg tacatatcaa
     3721 cgtcaccgtt caggtgcgcc gcttcgtgga aggttttcgg gttatcttca cgcatggtgg
     3781 agcgcagatg ggtgaagtaa atacgcggac cgaactgctt gatcatatca accagatcgt
     3841 tgtcagcacg cacgccgtag gaaccggtgc acatggtgaa accgttcgcc atgctgttta
     3901 cggtatcaac catccactgc atatcttcaa tggtggaaac aatgcgcggc aagccgagga
     3961 tcgggcgcgg cggatcgtcc gggtgaaccg ccatacgcac gccaacttct tcagcaactg
     4021 gaataatcgc tttcaggaag acggcaaagt tttcgcgcag tttggctttg tcgatatctt
     4081 tgtacagctc caggtgctta cggaactggt cgagggtata cccttcttcc gcgcccggca
     4141 gaccggcaat gatgttacgc gtcagacgcg ctttgtcttc gtcgctcata gtggcgaagc
     4201 gttcagctgc ctgagcaatt tcttcttcgg tgtaatccgc ttccgcgcct ggacgtttca
     4261 ggatatgcat ttcgaatgca gcgaattcga tctggtcgaa gcgcagagct ttggagccgt
     4321 ctggcagcac gtattcgagg tcagtacggg tccagtcgag caccggcatg aagttgtagc
     4381 acacggtgcg aatgccacac tgcgccaggt tgcgcagagt ctgctgatag ttagcgatcc
     4441 actgctcata gttgccagtg tgggttttga tatcttcgtg aattggtacg ctttctacga
     4501 cagaccacac caggcctgcg tcttcaacga tcgccttgcg tttgaggatc tcttctacgg
     4561 accatacttc gccgttcggg atatggtgca gcgcggtaac cacgccagtt gcgcccgcct
     4621 gacggacatc agctaaagaa accggatcgt ttgggccgta ccagcgccag gtctgttcca
     4681 tgatttttcc tctttcacat agtgggttcc acagcaaaga gacgcggaac cttggagttg
     4741 tttaggtatc cggatgaaca accagaccgc agccgtggcg ttgcatcgga tgagatggcg
     4801 tataagttct accttttggt aagacatgtc aaccagaatt ctgaaattgg ttaaccgcgt
     4861 cgcaagaatt tcactctcat ggttgttgct gcaaattgca gcaaagggta atcgatggtt
     4921 attgatcaga ggatgtgaca ttcatcgcaa caatggttga ccaatttaca taacatgtcg
     4981 cccaaaataa caacggttca accaagtaag gtaaaacgtt atgcatgtgc ttaacattct
     5041 ctgggtggta ttcggcattg gcctgatgct gatactgaat ttgaagttca aaatcaattc
     5101 aatggtggct ttgttggtgg cggcgctgtc cgtcgggatg ctggcaggca tggatttgat
     5161 gtcgctgctg cacaccatga aagcgggctt cggcaacacg ctgggagaac tggctatcat
     5221 cgtggtgttc ggtgcggtca tcggtaaatt gatggtcgac tccggcgcgg ctcaccagat
     5281 agcacatacg ctgctggcgc gtctcggtct gcgctatgta cagctgtcgg tgattatcat
     5341 cggccttatt ttcggtctgg cgatgttcta tgaagtggcc tttatcatgt tagcgccgct
     5401 ggttattgtt attgccgccg aagctaaaat tccgttcctg aaactggcga tcccggcagt
     5461 agcagctgcc actaccgcac attcactgtt cccaccgcag ccgggtccgg tggcgctggt
     5521 gaatgcttat ggcgcggata tggggatggt ttatatctat ggcgtactgg tgacgatccc
     5581 aagtgtaatc tgcgcaggtc tgatcctgcc gaagttcctc ggcaatcttg agcgcccaac
     5641 gccatcattc ctgaaagcag atcaaccggt agatatgaat aatctgccct ctttcggcgt
     5701 ttcgattctg gtgccgctga tcccggcgat cattatgatc tctaccacca tcgccaatat
     5761 ctggctggta aaagataccc ctgcctggga agtggttaac tttattggtt cctcgccgat
     5821 tgcgatgttt attgcgatgg tggttgcatt cgtactcttt ggcaccgcgc gtggtcatga
     5881 catgcagtgg gtgatgaacg cttttgaaag cgcggtgaag agtattgcaa tggtgattct
     5941 gatcatcggt gcgggtggcg tgctgaagca gaccatcatc gacaccggca ttggcgacac
     6001 catcggcatg ttgatgtccc acggcaatat ctcgccctac attatggcat ggctgatcac
     6061 tgtgctaatt cgtctggcga cgggtcaggg tgtcgtttcg gcgatgaccg ccgccgggat
     6121 tatcagtgct gcaatccttg atccagcaac cggtcagctg gttggcgtga atccggcgct
     6181 gctggtactg gcgacggctg cgggttccaa caccctcacc cacattaatg atgcatcttt
     6241 ctggttgttc aaaggttact ttgacctgtc agtaaaagac acgttgaaaa cctggggtct
     6301 gctggagctg gtcaactccg tggttgggct gattattgtg ctgattatta gcatggtagc
     6361 gtaaaagaac accagagcct gccaatggca ggctcagact gatgactaaa gtctaaaaat
     6421 gtgcccggac agtcccctcg cccctccggg gagagggtta gggtgagggg aacagaccag
     6481 taaagtgcgc tttgtttatg ccagatgcga cgctgacgcg tcttatctgg cctacaaagg
     6541 gctaacgtgc aggttttgta ggtctgatag cgtagcgcct caggtaccag cattagcaat
     6601 gcgctgtaat ttttttattg ataaacaaaa gtcacgccaa taatcgattg cacattccct
     6661 gcagtcacct gccctccggt acgtgcgtaa tttgccgtta atcccagact taccgccgaa
     6721 gttcctactg ctcctaacga taccgtgtta ttcgctggaa taatcgtacc gttgcgcgtc
     6781 aactgtacgc cgacgccctg cgctggtgaa aacgacgcgg tattggtgaa aatcgagttg
     6841 cccgcatctg cggttgtgcc ggagaggtaa taccccaggt tttggctttt cgcacaataa
     6901 acggtaagag gaattggcac tgaaccaggg tagtccggca gagtaacggt gacatcacga
     6961 gcagaaacat cacaaccgcc agtgggcacc accacatcat tattggcgta aatattccac
     7021 acaaactgaa aatcatcgct gttatagttg ttggtctgtc gcaaaataag cacggcaatt
     7081 aatgaaccag ctttaatagc caccccgccc gcactgctca caggcgtcaa ataaagcgcc
     7141 accggccacg gcttatccgt tctcgaatta taaacaaccc gcggcgtttc gctggtggtc
     7201 ggaaatggat agctactgcc actatatttt acggtcccgg aaaaattaga taacacgccg
     7261 ccgtaagccg agcctcgttg cagtgtgaca tagtctgtaa tggtttccgg ataatcgtta
     7321 tggcaaaaga tttgcgtcga aagatctacg accaggtttt gccccacatt cacgacaggc
     7381 gcaaggttta cataaacatt agcgctgcca ccgccaatag ggatagcggt accattggcg
     7441 gttttacagg cgaatgacca ggcatttacc gaccagccca tcagcagtac agcaaacagg
     7501 gtaatagctc gtttcattac aattatctct tcgggttcag ctgtaggtat aggtgatgct
     7561 aatcactgcc tgaatggttc cctgagtggc tccgccattt accgtcaatg ctctgacctg
     7621 taacgggaag tgcgctgatt gtgaggaatc atccacctga actgttttgg ttgcgccagt
     7681 attcaatgtg ttgccactgt catcctgtag ctctaactgg atgttttgcg cggtcccctg
     7741 gtttttataa tatccggtgc tgtcggctgc cccgctgaag ctggcagtga ccctcgacgt
     7801 tcccaccgga caattagtca actcaagcgc aacatcatgc caggccgatg ccgccccggc
     7861 agacataaga ctgaaagaat aaagatcgcc gagatctacc gtggcattgg tggtggaaac
     7921 cgtacacggt ttggcgacga ccttaccgtt caccgtgatg gtgacatcgg ctgcctgtat
     7981 cgtcgcactt gcgagcgcca acattgccgc caatacatac ccacgtttgc accatttcat
     8041 gagcatctcc agttactgat attcaagagt gaaggtagcc gtagcattga tatgccccgc
     8101 agtgacaggc acctgtgtcg ccattagccg ggcgtaaaaa ttcagcgtat ttggtttacc
     8161 cggcgtcagg gtcgtccacg aaatcgcaga cgatggagca ttaaggggta tttgattttg
     8221 ctgctcattc agaagctgta ttcccagtcc cgcagccgct gacaccgtat tttcaagtgc
     8281 aagcaggttg gcattgtggc tatctgcaac gccggtaaac ccaactttta cggcagaaac
     8341 ggcattacca cagggtgaca gcaaaatacg aaatggaacg acaggagtcg tcgcgccaat
     8401 gttgttaaat tgcttcgccg cgttttccat cagatcaaca gtaaaattgg ttgattcagc
     8461 ggccacacta cagccgttat ctctgacata gccgcggata gtaatcgtgc tatccgcagc
     8521 caaaacgcga cttaccgcca gccacaaaaa agcgcacaga agataaaaag gtttgtttct
     8581 catcacgccc ccttaacgac attcagctga tagctgggtt aataactgct gctgactctc
     8641 tggtggcagt tgataattgg cgacacagtg agcattttcc tcttctcccc atttcacctg
     8701 aacttttccc gctaaaggca ttccgctgag gtaaacctga ccattatccg caacaatgcc
     8761 gctactctgg ctactctctg atgtcaccat cgccccaaac ggcagcggct tattattgtg
     8821 agttagcgtc atgagcagtt ttatcccaac gcgcgcttta aactctgctc gcacgatcgc
     8881 cccacgagtg ggaacaacgt tagcgaccgc gttatctaaa tcgacgttat cagccaaggt
     8941 attggtatcc agcgccactc tattttcccg atattcagtg gcataaggca gcacggcata
     9001 accacgccag tcggtacgca cccccgtctg gttttcgact tttgcatctt ttgcgccagg
     9061 cgctttaaca agcaccaccg tatcgtttaa cggctgcccc agcgttacgc cattggcatg
     9121 agccaatacc ccaccgctga ctccgtaata gagctgctta atatcatcgc tatggctgta
     9181 accgatattg gcattgccgt aaccaccgcg ataattcagc gtggcgtagc ctgtacttcc
     9241 gctattacca tcgcctcccc cggcatagcc ggtttgcacg ctataactga ggttgttgtc
     9301 ttccagcaac gtaccgtata caccagccag attggtcatc cgaccgttga gatcgtgtga
     9361 catgctgtag ctggcactgg catgtcgcca ctgagattta ctgtcagaac gcagccagtg
     9421 gctgaaagga atattgacgt taagcgctaa catctgatcc cgtccttttt gccaggcgtt
     9481 tttcgtcagg ctatagctga gcgtccagtt gatatcttcg aacgcagtat ttaatccagc
     9541 ctggaattgc tcatcgacat tactcgttcc ccaataagtt tgatggctac cactcaaata
     9601 cagtgttgat gtgcgcccga gttgctgagt aacggtgagc tgtaatttcc cgcgtttgtt
     9661 ataagcgagg ttgtaatagt cggtgaattt cggcttaacc tgaataactc catcctgtgt
     9721 ttcgatgttg tagccattca ttcgactgta tgttgtatca gcgaaattaa aatatccgct
     9781 ggtcgaataa cggtaaccca ctaactgaat attcgtgcct gattcattga gcgatttgtt
     9841 atagagaaaa cgcaccgatt gtccgtcatg ctgactgtca tcgggaagtg tggaattagc
     9901 ctgcgtcata tccacagaca gagcgcccag tgcctccatg tttttcccga taccgaaatt
     9961 aaaagcacga taacgatccg ccagttgcgt tccaccatat attgtccagc cagccggaag
    10021 gccgtggagt aatgtactct ggaaaaagcg gggtttttcc tgctgcgcat ttccactacg
    10081 gtattctcct gccgtaatgg aataacgagt atgcccttca cgttgcaaaa gcgggactga
    10141 cgaatagggt acggtaaaaa tctgcgtgct gccgtcagcc tctttgatcg ttacctgcaa
    10201 gtcaccacta ttacctgcgg catagatatc gttgatggta aaaggccccg gtggcaccgt
    10261 actattataa atgccatatc cattttgttt aatagtgacc tgtgcagtac cacgagcaat
    10321 accgtggatc accggggcaa atcctctttg gctatcgggt aacatattgt catctgaggc
    10381 caattgtgcg ccgcgaaagt taataccatc gaaaatatcg ccctgggtat aaccatcacc
    10441 caacgtcagc cgggaacgta aaggaattat gtctcgctca atccaggtaa tgatatgctg
    10501 ccatttattt ttgctacctg atgaactgtc gctactgtta taactccagg tggtattgtc
    10561 gcgtaaacgc cacgcaccaa tatttaaccc actctgtagg tttaaatatg cataatggct
    10621 gttaccccca atccga
//
LOCUS       DAZPAB010000129        10532 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZPAB010000129 DAZPAB010000000
VERSION     DAZPAB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.129"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..2114
                     /locus_tag="ACHUPG_002893"
     CDS             <1..2114
                     /locus_tag="ACHUPG_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR0862424.1"
                     /translation="CGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYREL
                     NKAAQSSITAPDRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDK
                     YVAYAINIPDYELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQC
                     EIITPKKPGEAILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEK
                     GYWFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDL
                     DYPSADIRAKISKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEAS
                     KDNISKDEWKNLHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAK
                     HSKHEQIERSITPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLL
                     YERDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNN
                     GNLFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEA
                     WLNKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGL
                     VIINKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNIN
                     IPRRSALLLGKTGEPPNYLYL"
     gene            2450..3382
                     /gene="glaH"
                     /locus_tag="ACHUPG_002894"
     CDS             2450..3382
                     /gene="glaH"
                     /locus_tag="ACHUPG_002894"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR0862425.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3402..4670
                     /gene="lhgO"
                     /locus_tag="ACHUPG_002895"
     CDS             3402..4670
                     /gene="lhgO"
                     /locus_tag="ACHUPG_002895"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR0862426.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4693..6141
                     /gene="gabD"
                     /locus_tag="ACHUPG_002896"
                     /pseudo
     CDS             4693..6141
                     /gene="gabD"
                     /locus_tag="ACHUPG_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            6149..7435
                     /gene="gabT"
                     /locus_tag="ACHUPG_002897"
                     /pseudo
     CDS             6149..7435
                     /gene="gabT"
                     /locus_tag="ACHUPG_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            7673..9073
                     /gene="gabP"
                     /locus_tag="ACHUPG_002898"
     CDS             7673..9073
                     /gene="gabP"
                     /locus_tag="ACHUPG_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR0862427.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9094..9756
                     /gene="csiR"
                     /locus_tag="ACHUPG_002899"
     CDS             9094..9756
                     /gene="csiR"
                     /locus_tag="ACHUPG_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR0862428.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9757..10206)
                     /gene="kbp"
                     /locus_tag="ACHUPG_002900"
     CDS             complement(9757..10206)
                     /gene="kbp"
                     /locus_tag="ACHUPG_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR0862429.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10290..10448)
                     /locus_tag="ACHUPG_002901"
     CDS             complement(10290..10448)
                     /locus_tag="ACHUPG_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR0862430.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2645 a         2621 c         2729 g         2537 t
ORIGIN      
        1 cttgtggagt ttatcctcag gatttaaaag acagagaaat tatatcaact tttcacgcag
       61 aaaaagtcat taatgatctg ttaaaaaacg attataaaat ttccctttct ccagatacaa
      121 cttaccgaga gctgaataaa gctgcacaga gtagcattac agcgccagac aggataggag
      181 aagggaaaac atgggtttat caacgagata caatggttga aagaggtgat aacagtggtg
      241 ttcatcagta tggtccagct gaacatttca ctcacattat atctgacaaa ccgtccccaa
      301 aagataagta tgttgcatat gctattaaca ttcctgacta tgagctagca gccgatgtat
      361 ataatattaa cgtgacgtca ccttccggac agcaagaaac atttaaaata ctaatcaatc
      421 cagaacatct acggcaaaca cttgagcgta aatctcttac tgctgttcag aaatcacaat
      481 gtgaaatcat cacccccaaa aaacctggcg aagcgattct tcatgctttt aatgccacct
      541 accagcaaat cagggaaaat atgtctgagt ttgcacgttc ccattatggg tatatacaaa
      601 tccctccagt gacaactttc cgcgccgacg gaccagaaac tcccgaagaa gaaaaaggtt
      661 actggtttca cgcttatcaa cccgaagatc tttgtaccat ccataatcca atgggggatt
      721 tgcaggattt tatcgcattg gttaaagatg ctaaaaaatt tggtatcgat atcattcctg
      781 attatacctt taactttatg ggaatcgggg gaagtggtaa aaatgacctg gattatccct
      841 ctgctgatat acgagcgaag atcagtaaag atatagaaag tggtatccct ggctattggc
      901 aaggtcaggt tttgattcca ttcattaaag atccagtaac aaaagaacgt aaacaaatcc
      961 atccagaaga tatacatctc actgcaaaag acttcgaagc aagtaaagat aacatctcta
     1021 aggatgaatg gaaaaacctc catgcattaa aagaaaagag tttaaatgga atgcctaaaa
     1081 caacccccaa aagtgaccag gttattatgt tgcaaaatca atacgttcgt gaaatgcgaa
     1141 aatatggcgt acgaggttta cgttatgatg cagcaaaaca ctcaaaacat gaacaaatag
     1201 aaagatcaat aaccccaccg cttaaaaatt ataatgagcg attacacaat actaacttat
     1261 ttaacccaaa atatcatgaa aaaaccgtta tgaattacat ggaatatctg gtaacttgtc
     1321 agttgaatga agaacaaatg tcatcgctgc tttatgaaag agatgattta agcgccattg
     1381 atttttcatt gctcatgaag acgataaaag ccttttcatt cgggggagat ctcctaaccc
     1441 ttgcatcaaa accgggttcc acaatctcaa gcatcccatc agaaagacgg atattgatta
     1501 acattaacca cgattttcct aacaatggca atcttttcaa tgactttcta tttaaccatc
     1561 aacaagatga acaattagca atggcatata tggccgctct cccgttcagc aggcctttag
     1621 tttactggga tggccaggta ttaaaatcaa cgactgaaat taaaaattat gatgggccca
     1681 cgcgtgtcgg tggtgaggcg tggcttaata aaggttgctc tacctatcag cagctctaca
     1741 atgaattcca cgcattatat atagataaag caggaatatg gagcgcattt gagggtgtat
     1801 ttgcaactaa aaacgttctg gcctttagtc gtggggattc tgtgaacatt aatcactctc
     1861 ctcatgatgg actagttata ataaataaag gaaacgaaga agttgaaggt acctggccta
     1921 acaaattgca acctggaata tataaaaaca tggggagtaa tagcgttaac attattatta
     1981 ataatacccg aaaaattatc cccccttgta aagtatttac gcttagaggc ggatctctaa
     2041 atatcaatat tcctaggcgt agcgctcttc ttttagggaa aacaggagaa ccgccgaact
     2101 atctctattt ataatttaat gttatatctg ccccgataaa acggggcaga taatatgttt
     2161 agtttactaa cggtcatttt gtagtgaagc catttactgt tttttatcga ccagataatc
     2221 tgttctctaa tgttaactcc ccctaacctg ttgctttagt tattcatttc ctgtctcact
     2281 ttgccttaat accctacgtt aaatgttact aatttgttgc ttttgatcac aataagaaag
     2341 caatatgtcg cttttgtgcg catttttcag aaatgtagat atttttagat tatggctacg
     2401 aaatgagcat cgccatgtca ccctacatct cataagagga tcgcttctga tgaatgcact
     2461 gaccgccgta caaaataacg ctgtcgattc aggccaggac tatagcggat tcactctcat
     2521 cccgtcggcg caatccccgc gtctgctgga actcaccttc accgaacaga cgaccaaaca
     2581 gtttctcgaa caggttgccg aatggcccgt gcaggcgctg gagtacaaat cgtttctgcg
     2641 ttttcgggta ggcaaaattc ttgacgatct gtgtgcgaat cagctgcaac cattgctgtt
     2701 gaagaccctg ctaaaccgtg ctgaaggtgc gctgttgatc aatgcggtag gtatcgatga
     2761 tgtggcgcag gcagatgaga tggtgaagct ggcaacggcg gtagcgcatc tgattggtcg
     2821 ctcaaatttc gatgcgatga gcggtcagta ttacgcgcgt ttcgtggtga aaaatgtcga
     2881 taactcagac agctatctgc gtcagccgca ccgcgtgatg gaactgcaca acgacggcac
     2941 gtatgtcgaa gagatcaccg attacgtgct gatgatgaaa atcgatgact gggaacatct
     3001 ggaccactat ttccgccatc cactggcgcg tcgcccgatg cgctttgccg cgccgccgag
     3061 caaaaacgtc agcaaagatg ttttccatcc agtgttcgac gttgatcaac agggtcgccc
     3121 ggtgatgcgc tatatcgacc agttcgtcca gccaaaagat tttgaagaag gcgtgtggtt
     3181 gagcgagctt tccgatgcca ttgaaaccag caaaggcatt ctttctgtac ccgttcccgt
     3241 tggcaaattc ctgttgataa acaacctgtt ctggctgcac ggtcgcgacc gctttactcc
     3301 gcacccggat ctgcgccgtg aactgatgcg tcagcgtggc tatttcgctt acgccactca
     3361 ccactaccag acgcatcagt aagcgcaaag gaattgagcg gatgtatgat tttgtgatta
     3421 ttggcggcgg catcatcggc atgtcgaccg ccatgcaact gattgatgtt tatccggatg
     3481 cccgcattgc gttgctggaa aaagagtccg gcccggcctg tcaccagacg ggccacaaca
     3541 gcggcgtgat ccatgccggg gtctattaca cgcccggcag cctgaaggcg cagttttgcc
     3601 tggcggggaa ccgtgccacc aaagcctttt gcgatcaaaa cggcattcgc tatgacaact
     3661 gcggcaagat gctggtcgcc acgtctcagc tcgaaatgga acggatgcgc gccttatggg
     3721 aacgcacagc ggcgaacggt atcgagcgcg agtggataaa cgccgtggaa ctgcgcgagc
     3781 gcgaaccgaa tatcaccggg cttggtggca tttttgtgcc gtccagcggc attgtcagct
     3841 accgcgaagt gacggcggca atggcaaaaa tcttccaggc cagaggcggc gagattattt
     3901 ataacgccga agtcagcgcc ctcagtgagc ataaaaacgg cgtggtgata cgcacccgtc
     3961 agggcggtga atatgaagca tcgacgctga ttagctgttc cgggctgatg gctgaccggc
     4021 tggtgaaaat gctcggcctc gaaccgggct ttattatctg cccgttccgt ggcgagtatt
     4081 tccgccttgc gccggagcat aaccagattg ttaaccacct gatttacccc attcccgacc
     4141 ccgcgatgcc atttttgggc gtgcatctca cccgcatgat cgacggcagc gtgaccgtcg
     4201 ggccaaacgc ggtgctggct ttcaaacgcg aaggctatcg caagcgcgac ttctcattta
     4261 gtgacacgct ggagattttg agctcgtcgg ggattcgccg ggtgctgcaa aaccatctac
     4321 gctcaggact gggcgagatg aaaaactcgc tgtgcaaaag cggctatctg cggctggtgc
     4381 aaaagtattg tccccggctt tcgttaagcg atctccagcc ctggcccgcc ggtgtgcggg
     4441 cgcaggcagt atcgccggac ggcaagctga ttgacgattt tctgtttgtc accaccccac
     4501 gcacgatcca cacctgcaat gcgccctccc cggcagcgac atcagcaatt cctattggtg
     4561 cgcatattgt cagcaaggta caaacgctgt tggcaagcca gagtaacccc ggacgcacgc
     4621 tgcgagcggc acgtagtgtg gatgccttac acgccgcatt taatcaataa cctttgaaaa
     4681 caggacgtaa caatgaaact taacgacagt aacttattcc gccagcaggc gttgattaac
     4741 ggggaatggc tggacgccaa caatggtgaa gtcatcgacg tcaccaatcc ggcgaacggc
     4801 gacaagctgg gtagcgtgcc gaaaatgggc gcagatgaaa cccgcgccgc tatcgacgcc
     4861 gccaaccgcg ccctgcccgc ctggcgtgcg ctcaccgcca aagaacgcgc caccattctg
     4921 cgcaactggt tcaatttgat gatgcagcat caggacgatt tagcgcgcct gatgaccctc
     4981 gaacagggta aaccgctggc tgaagcgaaa ggcgaaatca gctacgccgc ctccttcatt
     5041 gagtggtttg ctgaagaagg caaacgtatt tatggcgaca ccattcctgg tcatcaggcc
     5101 gataagcgcc tgattgttat caagcagccg attggcgtca ccgccgccat cacgccgtgg
     5161 aactttccgg cggcgatgat tacccgtaaa gccggtccgg cgctggcggc aggctgcacc
     5221 atggtgctga agcccgccag tcagacgccg ttctctgcgc tggcgctggc ggaactggcg
     5281 attcgcgcgg gcattccggc tggcgtgttt aacgtggtca ccggttcggc aggcgtggtc
     5341 ggtaatgaac tgaccagcaa cccgctggta cgcaaactgt cgtttaccgg ttagaccgag
     5401 attggccgcc agttaatgga acagtgcgcg aaagacatca aaaaagtgtc gctggagctg
     5461 ggcggcaacg cgccgtttat cgtctttgac gatgccgatc tcgataaagc cgtggaaggc
     5521 gcgctggcct cgaaattccg caacgccggg caaacctgcg tctgcgccaa ccgtttatac
     5581 gtgcaggacg gcgtgtatga ccgctttgcc gaaaaattac agcaggcggg gagcaaactg
     5641 cacatcggcg acgggctgga gaaaggcgtc accatcgggc cgctgatcga tgaaaaagcg
     5701 gtagcaaaag tggaagagca tattgccgat gcgctggaga aaggtgcgcg tgtggtttgc
     5761 ggcggtaaag cgcacgaacg cggcggcaac ttcttccagc caaccattct ggtggacgtt
     5821 ccggccaacg ccaaagtgtc gaaagaagag acgttcggcc ccctcgcccc actgttccgt
     5881 tttaaagatg aagccgatgt gattgcacaa gccaatgaca ccgagtttgg tcttgccgcc
     5941 tatttctacg cccgtgattt aagccgcgtc ttccgcgtcg gcgaggcgct ggagtacggc
     6001 atcgtcggca tcaataccgg cattatttcc aatgaagtgg ccccattcgg cggcatcaaa
     6061 gcctcgggtc tgggtcgtga aggttcgaag tatggcatcg aagattactt agaaatcaaa
     6121 tatatgtgca tcggtcttta actggagaat gcgaaggaac agcaataaag agttaatgca
     6181 gcgccgcagt caggcgattc cccgtggcgt tgggcaaatt cacccgattt tcgctgaccg
     6241 cgcggaaaac tgccgggtgt gggacgttta aggccgtgag tatcttgatt tcgcgggcgg
     6301 gattgcggtg ctcaataccg ggcacctgca tccgaaggtg gtggccgcgg tggaagcgca
     6361 gttgaaaaaa ctgtcgcaca cctgcttcca ggtgctggct tacgagccgt atctggagct
     6421 gtgcgagatt atgaatcaga aggtgccggg cgatttcgcc aagaaaacgc tgctggttac
     6481 gaccggttcc gaagcggtgg aaaacgcggt aaaaatcgcc cgcgccgcca ccaaacgtag
     6541 cggcaccatc gcttttagcg gcgagtatca cgggcgcacg cattacacgc tggcgctgac
     6601 cggcaaggtg aatccgtact ctgcgggcat ggggctgatg ccgggtcatg tttatcgcgc
     6661 gctttatcct tgcccgctgc acggcataag cgaggatgac gctatcgcca gcatccaccg
     6721 gatcttcaaa aatgatgccg cgccggaaga tatcgccgcc atcatgattg agccggttca
     6781 gggcgaaggc ggtttctacg ccgcgtcgcc agcctttatg cagcgtttac gcgctctgtg
     6841 tgacgagcac gggatcatgc tgattgccga tgaagtgcag agcggcgcgg ggcgtaccgg
     6901 cacgctgttt gcgatggagc agatgggcgt ggcgccggat cttaccacct ttgcgaaatc
     6961 gatcgcaggc ggtttcccgc tggcgggcgt caccgggcgc gcggaagtga tggacgctgt
     7021 tgcaccgggc ggtctgggcg gcacctatgc cggtaacccg attgcctgcg tggctgcgct
     7081 ggaagtgttg aaggtgttcg agcaggaaaa tctgctgcaa aaagccaacg atctggggca
     7141 gaagctaaaa gacggattgt tggcgatagc cgaaaaacac ccggagatcg gcgacgtacg
     7201 cgggctggga tcgatgattg ccatcgagct gtttgaagat ggcgatcaca gcaaaccgga
     7261 cgcaaaactc accgccgaga tcgtggcacg cgcccgcgat aagggcctga ttcttctctc
     7321 ctgcggcccg tattacaacg tgctgcgcat ccttgtacca ctcaccattg aagacgctca
     7381 gatccgtcag ggtctggaga ttatcggtca gtgttttgat gaggcgaagc agtagcaccg
     7441 tccccatgca ggaggcgacg ctgcgcgtct tgtccggcct acgagaatcc aagtcggata
     7501 aggcgtttac gccgcatccg acaatctgta cgtgaacagg aataaatcta tgtcggccgg
     7561 ataaggcgga gccgctctcc ggcaaaaaga atcaataaca attaaacgcg tgacccgacg
     7621 cgggaaatgt cggggcgctc tcccaagtga cacactttcg agaggattca ggatggggca
     7681 atcatcgcaa ccacacgagt taggcggcgg gctgaaatca cgccacgtca ccatgttgtc
     7741 tattgccggt gttatcggcg caagtctgtt tgtcggttcc agcgtcgcca tcgccgaagc
     7801 ggacccggcg gtattactgg cctatctgtt cgccggatta ctggtggtta tgattatgcg
     7861 gatgctggcg gaaatggcag ttgccacgcc cgataccggt tcgttttcca cctatgccga
     7921 taaagccatt ggccgctggg cgggctatac catcggctgg ttgtactggt ggttttgggt
     7981 actggttatc ccactggaag ccaacatcgc cgccatgatc ctgcactcgt gggttccagg
     8041 cattcccatc tggttatttt ccctcgtcat taccctcgcc ttaactggca gtaatttatt
     8101 aagcgttaaa aactacggcg aatttgagtt ctggctggcg ctgtgcaaag tcatcgctat
     8161 cctggccttt attttccttg gtgcagtcgc aattagcggt ttttaccctt atgccgaagt
     8221 gagcgggatc tcaagattgt gggatagcgg cggctttatg cccaacggtt tcggtgcggt
     8281 attaagcgcg atgttgatca ccatgttctc gtttatgggc gcagaaattg tcaccattgc
     8341 cgccgcggaa tccgacatgc cggaaaaaca tattgtccgc gccaccaact cggttatctg
     8401 gcgtatttct atcttctatt tgtgctctat ttttgtcgtc gtggcattaa taccgtggaa
     8461 tatgccgggg ctgaaagccg ttggatctta tcgctcggtt ctggaattgc tcaatattcc
     8521 ccatgcgaaa ttaatcatgg actgtgtgat attactttcc gtaaccagtt gcctgaactc
     8581 ggccctgtat accgcctcaa ggatgctcta ctccttaagc cgtcgcggtg atgctcccgc
     8641 ggtaatgggt aaaatcaacc gcagtaaaac cccgtacgtg gcggtgttac tctccaccgg
     8701 cgcagcattc ttaacggtgg tggtgaacta ttacgcgcct gcgaaggtat ttaaatttct
     8761 gatcgacagc tccggcgcta tcgccctgct ggtttattta gtcatcgccg tttcacagtt
     8821 gcggatgcgc aaaattctgc gagcagaagg aagcgaaatt cgcttgcgaa tgtggcttga
     8881 tccctggctc acctggctgg tcatcggctt tattaccttt gtgttggtag tgatgctatt
     8941 ccgtccggcg caacagttag aagtgatctc caccggctta ttagcgatag ggattatctg
     9001 taccgtgcca attatggctc gctggaaaaa gctggtattg tggcaaaaaa cacccgttca
     9061 taatacgcgc tgataatgat caggagtcac accatgacca ttacgtctct ggatggctat
     9121 cgctggctga agaacgatat tattcgcggt aattttcaac cggatgaaaa attacggatg
     9181 agtttactga catcgcgtta tgcacttggc gttgggccgt tgcaggaagc actttctcaa
     9241 ctggtggcgg aacggctggt cacggtggtg aatcaaaaag ggtatcgggt ggcgtctatg
     9301 tcagagcagg agctgctcga tattttcgac gcccgcgcca atatggaagc gatgctggtc
     9361 agtctggcga ttgcccgcgg tggcgatgaa tgggaggcag acgttctcgc aaaagcgcat
     9421 ctgctcagta agctggaagc ctgtgacgcc agcgagaaaa tgcttgatga gtgggatctg
     9481 cgtcatcagg cgtttcatac agcaattgtg gcgggctgtg gttctcacta tttgctgcaa
     9541 atgcgtgaac ggttgtttga tctggcggcg cgttatcgat ttatctggct gcggcgaacg
     9601 gtgctttcgg tggaaatgct ggaggataaa cacgatcagc accagaccct gactgcggcg
     9661 gtactgccgc gagatacagc gcgcgccagt gagttaatgc gccagcattt actgacgcca
     9721 attcccatta tccagcaggc gatggctggc aattaactac tcttccggaa tacgcaacac
     9781 ttgccccgga taaattttat ccgggctttt tagcatcggt ttattcgctt cgaagatttt
     9841 attgtacaga ttagcgttac cgtagacctg tttggaaatg gcactcagag tgtcgccaga
     9901 cttaacggta taaaactggc tggcagtggc tggtgtcgcc gttttcacct gatcatcaac
     9961 actggcaata ccggaaatat tccccaccgc aacaaggatt ttctccttcg cctcctgact
    10021 caggccgtca ccagtgaccg tcgctttgcc gtcggcaatt tgaatgttca ctttatcggc
    10081 atcaggtata ccggttttgc tcagatgctc ctgcaccttc ttcgcctgat cgtctttatc
    10141 gtgctgacct gtaaccgcat cccagagttt ttctccggca tctttaacaa aattgaacag
    10201 acccatagct acctcattcg ttaacggaaa cactcagaaa gtgtagcaga ggaacgcggc
    10261 gcgttaacgc cgcgggggtg agcacaggtt taatcgcggg tttgcaccca gaacgcgtga
    10321 atcaaaccgg ggatataccc aagcagtgtc agcagaatat taataatgaa cgcccaaccg
    10381 aaccctttac cgagcagcac gccgatcggc ggcagaatga tggtgatgac gattctccag
    10441 aaacccatat gtactcccta taagaaaatt actcattgtt taaaaagaga ttttatctct
    10501 taagcgtagt aattttagcg gagcctgcca gt
//
LOCUS       DAZPAB010000130        10521 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZPAB010000130 DAZPAB010000000
VERSION     DAZPAB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.130"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(56..1387)
                     /locus_tag="ACHUPG_002902"
     CDS             complement(56..1387)
                     /locus_tag="ACHUPG_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0862431.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(1662..3026)
                     /gene="tdcG"
                     /locus_tag="ACHUPG_002903"
     CDS             complement(1662..3026)
                     /gene="tdcG"
                     /locus_tag="ACHUPG_002903"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR0862432.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(3098..3487)
                     /locus_tag="ACHUPG_002904"
     CDS             complement(3098..3487)
                     /locus_tag="ACHUPG_002904"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR0862433.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(3501..5795)
                     /gene="tdcE"
                     /locus_tag="ACHUPG_002905"
     CDS             complement(3501..5795)
                     /gene="tdcE"
                     /locus_tag="ACHUPG_002905"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR0862434.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(5829..7037)
                     /gene="tdcD"
                     /locus_tag="ACHUPG_002906"
     CDS             complement(5829..7037)
                     /gene="tdcD"
                     /locus_tag="ACHUPG_002906"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR0862435.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(7063..8394)
                     /gene="tdcC"
                     /locus_tag="ACHUPG_002907"
     CDS             complement(7063..8394)
                     /gene="tdcC"
                     /locus_tag="ACHUPG_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR0862436.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(8416..9405)
                     /gene="tdcB"
                     /locus_tag="ACHUPG_002908"
     CDS             complement(8416..9405)
                     /gene="tdcB"
                     /locus_tag="ACHUPG_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR0862437.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(9504..10442)
                     /gene="tdcA"
                     /locus_tag="ACHUPG_002909"
     CDS             complement(9504..10442)
                     /gene="tdcA"
                     /locus_tag="ACHUPG_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR0862438.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         2823 a         2753 c         2415 g         2530 t
ORIGIN      
        1 ataacggatt taaagtcgaa tcaaacatat taaaaacctt aaaatttcag gtaaatcaag
       61 aaaatgccag gaacagagaa acacaaagca acaaaccagt gacgataatc aggtacagag
      121 acatcccttt gtatttgtgc aatgccggta ctttgtaaac cagccacgcc gggatcaggc
      181 accctaccat gccgaaaatc gggctacaga tagaggtgaa gctcaacacc ggtgcgttca
      241 gtacgatggc gctccaggcc agcaaaatgg cgaaaatcat gatgccgcgc tgaacaagat
      301 tttcgttaat cttctcggca ggcatcttgc gacgcaggat gttcattacg atcccttgcg
      361 ttgcttcgcg aaagcctaaa tagacgccaa agaatgcagt catgacagca aagatattga
      421 gaatgacgct gaccactttc acccacgctg cgccgtcacc gctaataaac tgtgcggcaa
      481 tcgccagcgc ggaaatattc tgctcgtagg cttttaccgc ttcgtcatgt cccatcgcca
      541 gcgtgaacga cacggcgtag aaaaagacgg tgacaaacaa aatgccaaac gcgatattca
      601 ttgcccgcaa tgctttatgc cgcgccactt caattgattt ttcccgcgag cgataagaga
      661 tcaccatcgg gcttaacgtc tggataaaca gaatcgacgt cagggtaaac ggcagcgtaa
      721 taatggcgtt tttcaccagc agccccagcg gcggtagcga accgacgtta tacagatgcc
      781 acattcccac catcgacacg cccagcgcag cgactaccag cagcttggtc agcaccatgt
      841 cggtcgaaat tttgaataac aatttctcgc cgcgtgaaga gatcgccacc agaatgcaaa
      901 tcagtaccag accataaaag ggactgtctg acagcaaccc ttccgtcacg ccgaaggtat
      961 gcagatagga agcactatcg ttggtgatgg cggtggaata gacgaacatc caaatcacca
     1021 gcattacgaa atagagcgca cctaacagga tgccccagtt tttacctaaa taaccgctaa
     1081 tgacactcgg gtaatcttta cattctggtg attctgccag cgtattaata aacaaccgct
     1141 gaaacagata cattgccggg taaccaatca ccgatgagag caaaaatacc cacaatccca
     1201 tcaaaccgac ctgcaccggg agaaaaacaa tccccgcgcc aatcgccatc ccgatactca
     1261 taatcaccca gccggtgtca gtactgtcga atttgatcgc ttctcgccac tcgctctcac
     1321 tcattccagc acgacccgcc ggggtcgaag cgtctgcaat gacgccttta ttcgatgcaa
     1381 tttccataat ttctcgctca atattttgta gggcttatta tttttttccg agccgcatca
     1441 aggcgatatg cggtctacgt ttttgcaggc gagttattag aataaagaaa tgatacgcgc
     1501 gaggtcagag aaaatcttca caatcaagac tttaaaataa cggagatgga taagaatttt
     1561 ctactaaatt aatcacaaga gaatattcat tctctatttg cggcagatca caaaaaaaag
     1621 gtgcacattt gtgcacccaa ggatgaaagc tgacagcaat gtcagccgca gaccacttta
     1681 atggccagtc ctccgcgtga tgtttcgcgg tatttatcgt tcatatcttt gccggtttca
     1741 tacatcgtct cgatcacttt atcgagtgaa acacgcggtg ccgaggtgcg gcgcatcgcc
     1801 atccgcgcgg cgtttactgc tttcacggca ttaatggcat tacgttcaat gcacgggatt
     1861 tgtacctgtc cggcaaccgg atcgcaggtc agcccaaggt tatgctccat cgcgatttcc
     1921 gccgcattgc atacctgcgc cggactgccg cccagtagtt cagttaaccc tgccgctgcc
     1981 attgaacagg ccacgccaat ctccccctga cagccgacct ccgcgccaga gatggaggcg
     2041 ttcattttat acagcgcgcc aatagccccc gctgccagaa aatagcgggc aattgaccgc
     2101 tcgtttaccg gacgacggaa cttatcgtaa taagccagca ctgccggaat aatgccgcac
     2161 gcaccgttag tcggtgccgt taccacgcgc ccgccagctg cgttttcttc actaaccgcc
     2221 agcgcgtaca tgttgatcca gtcgatgaca ttcatcggat cgttagagat gttatcgcta
     2281 gaaaccagct gacgacgcag cgctacggca cgacacggca cattgagcgg accaggcagc
     2341 acgccttcag tgttcatccc gcgttcaata ccgtcatgca tcacttgcca gatacgggca
     2401 aaccctgcgt caatttccgc tttgctgcgc agcgccagct cgttgtgcat catcaggcca
     2461 gatatagaca ggccgttgta atcacacatt ttcagcagtt cacctgctga gtggaaatcg
     2521 taaggtacgg acgtttcgac atcgtgcgac aggccgaagt gttcttcttc gacaataaac
     2581 ccgccgccga cagagtaata ggttttactt aatagcgctt cctgtccctt ccaggcagtg
     2641 atccgcattc cgttctcatg gcgaggcaac atttcgggat ggaagataat gttctttgct
     2701 acaggaaaat caacaatatg cgcaccagat gccactggca gccgcccgct gcgcgttact
     2761 aactctataa atgcagggat ctcatcaatg acaacatcct gcggactgtt tcctgccagt
     2821 cccatgataa tggcgacatc cgtggcatgg cctttgcccg tcagtgacaa cgacccgtac
     2881 agatcgacca caatatggct cgtcgcggtt aataagccgc tactttccag ccgatcaata
     2941 aaactttttc cggcattcat tggccccacg gtatgcgaac tggagggacc aatcccaatt
     3001 ttgaaaatat cgaatgcact aatcatatcc acaccctcgg attgccgttc agtgaagtgg
     3061 agcggaacga ccttgcgacc gtcccgctca cgaggcttta cgcactacgt actgcgatag
     3121 cttcaatttc cagcttcaca tctttcggca aacgcgcaac ctgcacacag ctccgggtcg
     3181 ggtaggtcgc ctgatgctca tcgaagaact gcttatagac ttcattgatg gtggcaaaat
     3241 cattcagatc ggtgatgaac acggtcatct tgatgatatc gcccacgctc agcccggcag
     3301 caaccacgat cgctttgacg ttttcgaggc ttaaacgcgc ctgatcttgc acatcagccg
     3361 ggatctcacc ggtctgcggg caaaccggaa tttgcccgga ggtgaagacc atgctgccta
     3421 aatcaacgcc ctgaacataa gggccgattg cgcctggggc acgttgcgtt tcgataatct
     3481 ttttcataca tcctccggcg tcagagcgcc tgggtaaaag tacgtgaaat aacatcctgt
     3541 tgctgttcac gggtcagtgc gttgaagcgc acggcgtagc cagagacacg gattgtcagg
     3601 ttaggatatt tttccgggtg ctcgatggca tccagcagca tttcccgatt cattacgttg
     3661 acgttgaggt gttgaccgcc ttcgacatcc gcttcgtggt ggaaataccc atccagcagg
     3721 ccgacaaggt tggttttacg tactggatct tctttgccca gcgccgcagg aacgattgag
     3781 aaggtgtacg agatcccatc tttggcgtag gtgaacggca gtttcgccac cgacgtcaat
     3841 gaggccacgg cacctttgcg gtcacgacca tgcatcgggt tagcgcccgg cgcgaacggt
     3901 gttccggcgc gacgaccgtc cggcgtatta ccggttttct ggccgtacac cacgttagaa
     3961 gtgatagtca gaatcgactg ggtagggacg gcgttgcgat aggttggcag cgctttaatt
     4021 ttcttcataa agcgttcaac caggtcgcag gcaatgctgt ctacgcgctc gtcgttgttg
     4081 ccgtactgcg gatattcacc gtcgatttca aagtccaccg ccaggccgtt ttcgtcacgg
     4141 attggtttca cgcgggcata tttgatggca gacagggagt ccgtcgccac cgacaggccc
     4201 gcgatgccgc atgccatagt gcgatagaca tcacgatcgt gcagcgccat cagcgaagct
     4261 tcgtagctgt acttgtcgtg catgtagtga atgatattca gcgcgctgat gtactgcacc
     4321 gccagccagt ccatgaagtg atcgaggctg tccatcactt tgtcgtaatc cagcacgtcg
     4381 tccatcagcg gtgctgtttt cggcccgacc tgaatcttca gcttctcgtc caccccgccg
     4441 ttaattgcgt agagcagcgt tttcgccagg ttagcgcgtg caccaaagaa ctgcatttgc
     4501 ttaccaatca ccattgggct gacgcagcag gcaatcgcgt aatcgtcgct gttgaagtca
     4561 gtacgcatca gatcgtcatt ttcatactgc aaggaagagg tgacgatcga cacctgcgcg
     4621 gcatattttt tgaaggcaat cggtaattct tccgaccaaa gaatggtcag gttaggttcc
     4681 ggtgccggcc ccatagtgtg cagggtgtgc aaatagcgga aggagttttt ggtcaccagc
     4741 gtacgaccgt ccagccccat cccgccgatc acttccgtcg cccagattgg gtcgccggag
     4801 aacagcgaat caaattccgg tgtacgcagg aagcgtacca tacggatctt catgatgaag
     4861 tgatcgatca gttcctgtgc ctgctgctca ttgaggacgc cagctttaaa gtcgcgctca
     4921 atgtagatat ccaggaacga tgccgtgcgc cccagcgaca tcgcgccgcc attttgcgat
     4981 ttcactgccg ccagataagc gaagtagagc cactgcaccg cttcctgtgc attctgcgcc
     5041 gggcgagaga tatcaaagcc atatttcgcc gccatttcct gaatctgcaa cagcgcatga
     5101 cgatgctctg ccagctcctc acgcagacgg atggtggctt ccagatcctc gcctttttcc
     5161 agacgagact ggagatcggc aaattgcagt tcgcgttcac gcaccagata actgatgcca
     5221 tacagcgcta cgcggcgata gtcaccgata atgcgcccac gaccatagcc atctggtaaa
     5281 ccggtcagca cgccagattt acggcagcgc agcatatccg gtgagtaaac atcaaatacg
     5341 ccctggttat gggttttacg cagatcggta aacagatatt caaattcact gtccatttca
     5401 cggccatagg cgtggaatga acttttaatc atattaatgc caccgaacgg gtgtagcgca
     5461 cgtttcaacg gcgcatccgt ttgcaggcca acaatttttt ccagcggctg gttaatatat
     5521 cccgcatcat gagcggtaat tgtggtggca atattggtat cgaaatcaac cggcgcgtgg
     5581 gttgcgtttt cgatacggat gccttccatt accttttccc acaattccgt ggtggcaggc
     5641 gtcgcttcgg cgagaaaaga ttcatcgcct tcatacggtg tatagttatg ttgaataaaa
     5701 tcgcggacat taatttcgct tttccagtcc gtacctttaa agccaagcca tgcgtcagcg
     5761 tacagcttat cgctggtatc aatatctacc ttcatgaaaa ataatctctc tacaatactt
     5821 caactaaatt atgcaaattc tgcgggcgcg ttaactttac ctaaatgaat ggcatccaaa
     5881 gcaatcattt tttcttcgtt agtcggaata acgacgcaaa tgacatgcgc attttcactg
     5941 gaaacaattc gctcgccaaa agagttagaa cgattattca tttctgtatc aatctctacg
     6001 cctaatacag ccaaatgttc catgaccaga cgacgaatta aacttgaatt ctctcctatt
     6061 ccgccggtga atataattcc atccagccga cgtaatgaag ctgcatgtcc ggcaatatga
     6121 cgggcaattc ggtgaacaaa ggttttaatt gccagttgcg cgcgttcgtg accttcatgc
     6181 caggcttttt ccagaacacg taaatccgaa gaaagaccgg aaatacctaa taatcccgac
     6241 tctttattca ctacgcgttc caggtcaccc aggctctggt tggtttggct ggcgacccag
     6301 gacatcgcac caaagtcgac atcgccactg cgggtaccca tcatcaagcc ttccagcggc
     6361 gtcattccca ttgaggtgtc aacactctga ccgttgcgaa ctgcgcagat tgacgcgcca
     6421 ttgccaagat gcgccacaac caggccggag tcatcttccg ccagattcag cagcgaatgt
     6481 gcgcgctggg aaacatagcg gtgcgacgtg ccgtggaaac cataacggcg tacacctaac
     6541 tcttcataat atttccacgg caggccgtat aaataagctt ccggagccat cgtctggtgg
     6601 aaactggtat caaataccgc cacctgagtt acgcccggaa ataattgctg cgccgattca
     6661 ataccactta aatttgcgta attatgcagg ggtgccagtg gagaaacgcg acggatatta
     6721 tcaatgactt catcggtaat aatggcggac tcggtaaaaa tactgccgcc gtgagcgatg
     6781 cggtggccaa ttaaggccac gctgtcattt aaattccgtt tttccagttc aaatgcaatt
     6841 gccttcaatg caccttcgta gctgtggtga gccagcggtg ctggctctcc cccatttacg
     6901 gataagaatg cattttccga gttaataccg tcggcaatac ctgacattaa tacttcacaa
     6961 tcgctggcat cgagtacgga aaacttaatc gaagacgaac cacagttaat aaccaaaaca
     7021 accggaaatt cattcatctc ttttctcatc ctgagttacg gattaaaaca gtttgtatac
     7081 gatgttcagg atggtcagca gaccaatcac ggtaacaaac acgttatcca gacgaccacg
     7141 gtatttcgcc agagacggcg ctttacggat agcatacatc ggcaacaggc acagcaggga
     7201 tgcgataatc ggtgcgccca tggcttcaat caggtcgagg atgttcgggt tggcgtaggc
     7261 aacaacccag gtggagccca tgatgaagat catgctgaga gtattcagtt tacccagcga
     7321 cactttggtt ttgtcacctt tataaccgaa cttcagaatc agaccattca agccttccag
     7381 cgtccccaga tagtgaccga agaaagattt gaagatagcc acgagtgcga tgatggaagc
     7441 cgcatattcc agtgtaatcg cgaacgttgt tttcgtaccg gtcatggacg caaagtggtt
     7501 agccagataa gaaagcactg gaatattctg cgctttggct tccgccatgt tggccggaga
     7561 cagagtaaac aggcagctaa aggcaaagaa catcaccact gcaaccatca gcatgctggc
     7621 acgagaaatg atttgggaac atttacgttc ggtgaagtcg cgaccgaagt ctttctcata
     7681 ctcttcacgt ttagaaacca cgaaggaaga gacgattggc gagaagttaa aggagaaaac
     7741 catgatggaa atccccagcc agacagtgat caggataccg tcatgaccgg ttaacgacag
     7801 cgaaccgagg tcaacctggt cgataactgc agagttccag taagggatca gcgacagaga
     7861 aatcagcacc aggctggcga taaacggcca taccaggtag ctcatcactt taaccatcag
     7921 atccttacca aaccagatga cgaaagccat cagcagcaac aggaacagcg ccacaaagcc
     7981 gcgattcagc ggtgcaaatc cgagctggtt ttcccagaac gtcataaagg tattggtaat
     8041 agtaacgcca taaatccaca gcagtgggca aatcgcgaag aagtacagga acgtgataac
     8101 cacgccgcca gttttaccaa aatgctcttc caccgtttcc gtaatgttgc cggaagggtt
     8161 agagccagaa agacacagac gcgccagcgc ccggtggcaa taaaatgcga tggggtatgc
     8221 caataccaac atcagaagaa tcgggatcag tccgccaaaa cctgcgcgga tagggaagaa
     8281 cagcaccccg gcgccgattg ccgtaccaaa caagcctaac gtccatgtgg tatctgattt
     8341 acgccaggac gattgttttg tctggctgga tacaatgcta tctgaagtac tcatatccta
     8401 tcctcaacga attaattaag cgtcaacgaa accggtgatt tgagagacgc gagaaagatc
     8461 gatattgccg ccggaaataa tactgacggt ttttctattt tgaatatatt ggtctaattt
     8521 accgcttaat aatgcagcac atgccagagc gcctgcgcct tcggtgacga ctttattgcg
     8581 ctgaattaag gcaatcatac tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc
     8641 gactaattca cgaacgattt cgtaagttaa attacccggg cgggagacat cacaaccatc
     8701 cgccagggtg ccggtagttc ggtgcgtggt tatttctccg gagtggaaag aagccgccat
     8761 gccgtgaacg ttttctgact gtacgccaat aacacgaatg gtcgggttaa tagatttaat
     8821 tgccaccgca ataccagcaa ttaaaccacc accaccaatt ggcacaatca cgttatcgac
     8881 atcatagaga tcttccataa tttccagacc aatcgttccc tggccagcaa tcactttcgg
     8941 atcatcgtaa ggtgggataa aaatacgacc ttccatttcg acaatttcgc tcactttagc
     9001 gatagtgtcg ttgaagttat caccatgcag aacgacttct gcggagtagt cgcacgttgc
     9061 cgctactttg gattttggcg caccttttgg catcaccact ttaccgtcga tacccagcat
     9121 cgcgcaggag agcgaaaccc cttgcgcatg gttgcccgca gaacaggcca ccacgccttt
     9181 gcgtttttcc gcatcggtca gtgaacttaa tttattaaat gcgccacgaa ttttaaatga
     9241 acccgtacgc tgcatatttt caaacttcag gaatatttca cctttgcaac gttcactaaa
     9301 atagttggag cgaggcatgc ctgttttata aattcgccca gccagtcgtt gtttcgcttc
     9361 aataatatca tcaatagcaa ccggcagatc gtatgtaata tgcattataa aacctcttcg
     9421 cctgaattaa atatgtaggt aaccgtaacc gacacctgca aaacagacag gtggattatt
     9481 tatgtttaaa taaaaacaaa caactaacca acttctatta attgccttcg tctacaccca
     9541 ttataagatg aatactcttt ggctaattcc accaaaaccg atgctgcttt tttaatacga
     9601 taatttttcg accataccgc ggcatattgt gccacaggta atgtttcttc aaccggaata
     9661 gtaataaatt gattagaacc aaaaggtgac gtcatatcac aaggaattac agttaagaaa
     9721 tcagcattga gaacaagatt ataaattgtc acgactgagt cggttttaac gatgttttca
     9781 atactgatgc catttctttg taacgtagta agcagttcgc tgtagtaccc catattagtt
     9841 tgtggcaaca cccactgttc gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt
     9901 cgggacttac tggctaccag cacaaactcg gactcgaaca gcggctcaac atgtaaatcc
     9961 tgaagcttca tttctgcact taacgtacca atcgcaaaat ccagtcgacc gtcgcggatt
    10021 gccggtaaga acgaagacag ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc
    10081 tctttgaact tgttgatcat ccctgacata aaagtaaaac caatcaatga aggaaaacca
    10141 aatgagactt ccaccaccgc ttcagaagac ataccgctta tctcattaac catatttttc
    10201 atttcacggg taatggattc ggaacgggag agtaacaatt gaccggcagg tgttaatgtt
    10261 acaccggtat ttttccgcac cactaattcc acaccaaaat aatcttcaat atcgttaatg
    10321 attttactga cggccggttg agttaaccct aattcttttg cagccgagcc gatagagcca
    10381 cttctaatga cttcctgaaa gactaccagg tgctgcgttt tcggaagaag aatagtgctc
    10441 ataacgacct acgttaatta cctcattgac ggcatgaagt gtatcaaaat gaaataaaca
    10501 ggatatgtgc gaccactcac a
//
LOCUS       DAZPAB010000131        10464 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZPAB010000131 DAZPAB010000000
VERSION     DAZPAB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.131"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            2..202
                     /gene="cspG"
                     /locus_tag="ACHUPG_002910"
     CDS             2..202
                     /gene="cspG"
                     /locus_tag="ACHUPG_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR0862439.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            213..401
                     /locus_tag="ACHUPG_002911"
     CDS             213..401
                     /locus_tag="ACHUPG_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR0862440.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            376..606
                     /gene="ymcE"
                     /locus_tag="ACHUPG_002912"
     CDS             376..606
                     /gene="ymcE"
                     /locus_tag="ACHUPG_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR0862441.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            596..769
                     /locus_tag="ACHUPG_002913"
     CDS             596..769
                     /locus_tag="ACHUPG_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR0862442.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(818..1891)
                     /locus_tag="ACHUPG_002914"
     CDS             complement(818..1891)
                     /locus_tag="ACHUPG_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFR0862443.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1993..4704)
                     /gene="torS"
                     /locus_tag="ACHUPG_002915"
                     /pseudo
     CDS             complement(1993..4704)
                     /gene="torS"
                     /locus_tag="ACHUPG_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4787..5815
                     /gene="torT"
                     /locus_tag="ACHUPG_002916"
     CDS             4787..5815
                     /gene="torT"
                     /locus_tag="ACHUPG_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR0862444.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5788..6480)
                     /gene="torR"
                     /locus_tag="ACHUPG_002917"
     CDS             complement(5788..6480)
                     /gene="torR"
                     /locus_tag="ACHUPG_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR0862445.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHELSADLLVTQ
                     HGEGYFLAADVC"
     gene            6567..7739
                     /gene="torC"
                     /locus_tag="ACHUPG_002918"
     CDS             6567..7739
                     /gene="torC"
                     /locus_tag="ACHUPG_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR0862446.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7739..10279
                     /gene="torA"
                     /locus_tag="ACHUPG_002919"
                     /pseudo
     CDS             7739..10279
                     /gene="torA"
                     /locus_tag="ACHUPG_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10276..>10464
                     /gene="torD"
                     /locus_tag="ACHUPG_002920"
     CDS             10276..>10464
                     /gene="torD"
                     /locus_tag="ACHUPG_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR0862447.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2523 a         2686 c         2789 g         2466 t
ORIGIN      
        1 aatgactggt ttagtaaaat ggtttaacgc agataaaggt tttggcttta tcactcctga
       61 tgatggcagc aaagacgttt tcgtccattt caccgccatc cagagcaatg aattccgcac
      121 gctgaacgaa aatcagaaag ttgaattttc tattgagcag gggcaacgtg gccccgcggc
      181 agcgaacgtt gttacgctct aaggttgccg ttattactca acatctccat ttccgctgtc
      241 catgttgtca tggttcacag taccgtacat cggcattcga tgtgacggag cgaaaccctt
      301 tgggcgctaa gtgtattttt tgtaaatcga ctatgatcac ctttgataac gtcgcgctgc
      361 aaatacgcac tgaccatgcg ccgctggatt tcacaaaata atatcaggct ccctcgtgga
      421 gcctttttta tatctgcctt atttttcttc aacgctgtat gtatagtaag cgataacctg
      481 ttgattattg aatctttcgg gaagatggct tataacattt cttacctgac cagggtaccg
      541 ggaaccaaca ccttactggc gtgttgctgt ctttcaagac cagacgaggt taacagtgaa
      601 tattgaagag ttaaaaaaac aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat
      661 cgccgagctg aacaagaata cagggaaaga agtctctgaa attcgcttca tcgcacgaga
      721 aaaaatgacc gggcttgaaa gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca
      781 tcaccccgtg cgaggtgatg ttccgcttgt tgctaattta gtgaccaatc attggcgctt
      841 gtggaattaa gcgtcggtac aattcctccg gcaccgggct ttgccatact cccgcataca
      901 ttgcgtaacc aatcaccgca aacataatcc ccagaaccag tagcgtcatt aaccagccag
      961 acaacgcaaa ggctttttta tttgctgcag gtttttgcag tgaaaaggtc aatgttgagg
     1021 ctaccggaca tgactctacg caagtcatac agccggtaca ttccactgtt cgtacctgaa
     1081 ttaatttatc taccgggatc cgtgatgggc acttttttgc gcatttgcca caatcgatac
     1141 aactttcggc attgcgacga atcttaaacg gcgacaatag cgaaaccacg cccatcagcg
     1201 cgccatatgg gcaaagataa cgacaccagg catggcgaat aaacaggctg gcaatcagca
     1261 aaacggtcac gctgattaat gtcgcggtcc ccatatgacg aaagaaatcg agcattttaa
     1321 cgtccatcac cacgctatag ggcgacaaca taaaatagtg aatcgcctga gcgggcatca
     1381 ataacgcgat atagagaaat aaactcaaca gcaaatactt cacgccgcgc agaggaatat
     1441 ccagccagcg gggaaggaca cattgccgac caaacagttt gttaccgaga tcgccgatta
     1501 attcagaaag cgtaccaacc gggcataacc atgagcaaaa ggcctttttg agtaatagac
     1561 tgatgacgat aaaagcgacc aataacagca tcgcggcggc gtggacggac ggtaactgac
     1621 ctgttgcaag gctatatttc agattcatca gaccggcaat cggtagccag ccttcgatac
     1681 ctcccggtct ggcgacaaat gtcgtactac ctgccgtttc gtaatagcgc acccaatacc
     1741 agaacgtgat ggcaatataa atattcattg ccaatagtaa gaattgcgtc gctttacgcc
     1801 aggtcgtggc attgcgccag tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc
     1861 gctgccagcg ggttcttttt ttctctgcca tgattttgcc agtccgttaa gttgcatacc
     1921 aaatgccact attctagtta ttcttaactg gctgatattg attcaaatcg cgttcaggtc
     1981 tttcttattc aatcatgctt ccagagcggc aacactgcgt gtaatttctt cgtgtggaag
     2041 gggggcagat aatggctgct gctccagttg tgcgcatagc tgactggcgc tacgcattcc
     2101 cagactcgag caactgcttt ttagctgatg cgcggcacgc tttattttct cgctgtcctg
     2161 gctggcgcgg gcaatgtcga tttcatcgag aagcggcagg gcatgttgtt taaataatgc
     2221 cagccattcg tggatcttct ccgtccccat taactgagcg tcttcattga gttgcgagac
     2281 atccagtggt agatcgttat tggcttgcag ttggagatag tgcgccagta actgaccgag
     2341 cacttcacgc ggcaccggtt tagggataat cccgcgaaat agtgaactgg tacgctggcg
     2401 cagcgtttcg tcaatgacat gggcgctaaa gccaatcaaa accagcgacg gatattgctg
     2461 tgccagttgc cgggcaaggg taatgccgtc gatatccggc agatcaaaat ccaccagtgc
     2521 ggcagcaaac ggttcgctat tttgcagtgt ctctaaagcc tgcgcggcat tgccaatagc
     2581 aacaacctgc gcaccactgg tgttcagcat ctcaacggta attcgctggg ttagcgggtt
     2641 atcttcaatt aacagcaaac gtaaaccgtc aagacgcacc gcctgattgg ctgtttttgg
     2701 cacgggtgca gtggcaacac gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg
     2761 cgtgctggtg gcgctcagtt cgccgcccat cgcctgggcc agacggctac tgatagtcag
     2821 ccccagcccg gtgccgccgc gtttgccgct tacctgcaca aatggctgga agatttctgc
     2881 cagtttcgcg ggatcaatac cgcagccgct gtcttccact tcgaccagcc attgctcgcc
     2941 atcagtgcga ctacgcagga taatgtgccc ttcgtcagta aaacgcaggg cattgctcaa
     3001 taggttggta atgacctgac gaatacgtcg tggatcgccc attaacgcgc tcggtacatc
     3061 atcggcaatt gccgttgcca ggcgaatcgg gcgacctttc acccgtccgc tcattaattg
     3121 cagggtactt tccagcagcg ggcgcggttc aaagggctca tcgctgactg aaacattttt
     3181 gccacctgct tcgatagccg aataatcgag aatatcgttg aggatggtca gcaacgattc
     3241 gccagagtcg gtaatcgcct gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc
     3301 cagcagttga gcagtgccga gaataccgta cagcggtgtg cggatctcat ggctcatcgc
     3361 cgtcagaaac gccgattttg cctggctggc tttttctgct tccgcccgtg cctgtcggtg
     3421 ttctatcacc agttcctgca attcagctgt acgcgctttg acctgcgccg ccagctgttc
     3481 gcggtggcga ttcagtgcat gaacactgct gcgaaacgca tccatcagcc gcccgatggt
     3541 atccagctcc cgtacgccag cggtttccgg gaaaggggag tcaatatcac cgtccagcag
     3601 ccgttgcagc gcctgcgttt gttcggcaag tggacgcgtg aatgagcgat aaaccacgcg
     3661 ccagaggatc ataatcagtg cgcaaagtga aaccatcccc agcaataaca ggctgtattg
     3721 cccgcgtgca ctggcttttt ccagatgtgc cagtccatgc tgattgtgca gctcaatggt
     3781 gtcgaccagc tgactgactt cgctactaaa ctgcgcgaac tgggcgatat tattttgggc
     3841 gagagtttgc aggtgattgc tgatttcact gtcctgctgg aacagcgcca acagatcgct
     3901 atattggcta acggtagtta acgttgttgc gacctgcgca cgaactcccg gatcttcaat
     3961 gcgtatttgc cgacgttgca gaattttcac cgcattattg agctgctttt ccagcgttgg
     4021 tgcatttttc tggatctgct ccagccccag attcatcacc atttgctgca cccgcagagc
     4081 gctaaggcgc agttcattca tctggttaac atactcaaga tcgatatcaa tcagccgatc
     4141 gagtgcactt tcagcagcct gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg
     4201 ggtcgctcca gcggaagttg tcgcattatt cgcctgacct tgctccaggc gtgcgatctc
     4261 atcggcggca gctatctgct gactgagttg ccgttgttgc tggcgtagtt gcagacgccg
     4321 ccccaccagt tccccttgct gacgtaatga acgggagatc tcctgctcct gttgttcaat
     4381 agcggtggta tcaaaacctt gttcccgtaa cgcttgcagc aacgcattaa tcttcaggct
     4441 ttatgcggtg agcattcgcc cctgcgcctg ccacatcttt tcgttatcgg cactggtcag
     4501 gttctgcgcg gcgaaaagtt cccaggcgct ggcttcgctc aactggcgcg ccatattcat
     4561 ggtaggaatc aatgcctgag tattgtcttt ttccacctgg ctgataaagc gcaggttgta
     4621 ccatcccacc agggtactgg tcagggttaa cagcgccatc agggcaaagc ccatccagag
     4681 tcttcgggtc agggttaaat tcacggtcgg tgcacttaag gtgaaaaagg ttgagtcgca
     4741 aagtggaatg catctagcat aaagccttat tattgatgag gctatcatgc gcgtactgct
     4801 atttttactt ctttcccttt tcatgttgtc ggcattttcg gctgataacc tgttgcgctg
     4861 gcatgatgcg cagcatttca cggtgcaagc ctctatgccg cttaaagcca aacgcgtatg
     4921 gaaactgtgc gcgctttatc ccagcctgaa agattcatac tggttatcgt tgaactatgg
     4981 tatgcaggag gctgctcgcc gctacggtgt ggatttaaaa gtgctggagg caggcggcta
     5041 cagccagttg gctacccagc aagcacaaat cgaccagtgt aaacagtggg gcgcagaggc
     5101 cattttgctc ggtagtagca cgacgtcatt tcccgacctg caaaagcagg tagcaaatct
     5161 gccggtgatc gaactggtaa atgctcttga tgatccccag gtgaaaagcc gcgttggtgt
     5221 accctggttt cagatgggct atcaacctgg gcgatatttg gtgcaatggg cgcacggtaa
     5281 accactgaac gtgctgctga tgcccggacc cgataacgcc gggggcagta aggagatggt
     5341 cgagggtttt cgcgcagcca ttgccggaag cccggtgcgt attgttgata ttgcgcttgg
     5401 tgataacgat attgaaatcc agcgcaacct gttgcaggag atgctggaac gccacccaga
     5461 aatcgacgtc gttgccggaa cggccattgc ggcagaggcg gcaatgggtg aagggcgtaa
     5521 cctgaaaacg ccgcttaccg tggtgtcgtt ttatctttca catcaggtgt atcgcgggct
     5581 gaagcgggga agagtgatta tggctgccag cgatcaaatg gtctggcagg gggaactggc
     5641 agttgagcag gccatcaggc aattacaggg gcaatcggtg tctgataatg tcagcccacc
     5701 gattttagtt ctgacgccga aaaatgccga ccgagaacat atccgccgct cgctgtcacc
     5761 ggggggattt cgtccggtct atttttatca gcacacatca gcggctaaga aataaccttc
     5821 accatgttgc gtcaccagta aatccgcgct gagttcatga cgtaaacgac gaattaacac
     5881 atcgacggtg cgcaggtcag ggttttccac ccgacgcgca gaaagcatac gcagcagacg
     5941 ctcacggctg agaatttcgc ccggattagt cacaaatgcc accaacattt catactctgc
     6001 gcgggtcagt ttaatcggct cgccatcccg ctccagcgta tggcgcgaca cattcaggca
     6061 ataacccgca aagcgatagc agtagtcctg agtgtgcggt tgagcttgtc gcgcgaggtc
     6121 gattcgccag agcagatttt tcacccgtac taccagttcg cgcagttcca gcggtttggt
     6181 gacgtaatcg tctgcgccca tttccagccc aacaatacgg tcaatccgat cgctgcgtcc
     6241 ggtaaccaga ataatcccca ccgttgagcg ttctcgcagg gcgcgggtta acatcaggcc
     6301 attttcatcg ggtaagttga tatccagcag aattaaatct accggctgat tctgcataat
     6361 ttcccgtagc ccggcaccgc tcgccgtaac ggaaacggta tacccctcct gagtgaagta
     6421 ggattgtaat cgcgcctggg taaccggctt atcttcaaca ataacaatgt gatgtggcat
     6481 cagagggttt tactcattct gttcatatct gttcatattt gctcattaag atcgcttcac
     6541 taaaccataa ttctacaggg gttattatgc ggaaactctg gaacgcgcta cgccgaccca
     6601 gtgctcgttg gtcggtactg gcgctggtcg caattgggat tgtgattggc attgcgctga
     6661 ttgtattgcc acacgttggg atcaaagtca ccagcacaac cgaattttgt gtcagttgcc
     6721 acagtatgca accggtgtat gaagaatata aacagtcggt gcatttccag aacgcctccg
     6781 gcgtgcgagc tgaatgccat gactgtcata tcccgccgga tattccaggc atggtgaagc
     6841 gcaaactgga agcgagcaac gacatttacc agacctttat tgcccactcc attgatacac
     6901 ctgaaaaatt cgaagccaaa cgcgcggaac ttgccgagcg tgaatgggcg cgaatgaaag
     6961 aaaacaactc ggcaacctgt cgctcctgcc ataactacga tgcgatggat catgccaggc
     7021 agcatcctga agcggcacgt cagatgaagg tggcagcgaa agataatcaa tcctgcatcg
     7081 actgtcataa aggtattgcc caccagttac cggatatgag tagcggcttc cgtaagcagt
     7141 tcgatgagct gcgcgccagt gctaatgaca gtggtgacac gctgtactct attgatatta
     7201 agccaattta tgcggcgaaa ggcgataaag aagcctctgg ttctctgctg cctgcttcgg
     7261 aagtgaaagt ccttaaacgt gacggcgact ggctgcaaat tgaaattacc ggctggacgg
     7321 aaagcgccgg acgtcagcgt gtactcaccc aattcccagg taaacgcatc tttgttgcct
     7381 cgattcgtgg tgatgtgcag cagcaggtga aaacgctgga gaaaaccacc gttgccgaca
     7441 ccaataccga gtggagcaag ttgcaggcca ccgcgtggat gaagaaaggc gacatggtga
     7501 acgatatcaa accgatctgg gcctatgcgg attcgttgta caacggcacc tgtaacgagt
     7561 gccacggcgc accggaaatc gcccactttg acgctaacgg ttggatcggc acgctcaacg
     7621 gcatgattgg ctttaccagt ctcgataaac gtgaagaacg cactttgttg aaatatctgc
     7681 aaatgaatgc gtctgacacc gcaggtaagg ctcacggcga taagaaggaa gaaaaataat
     7741 gaacaataac gatctctttc aggcatcacg tcggcgtttt ctggcacaac tcggcggctt
     7801 aaccgtcgcc gggatgctgg ggccgtcatt gttaacgccg cgccgtgcaa ctgcggcgca
     7861 agcggcgact gaggctgtca tctcgaaaga gggcattctt accgggtcgc actggggggc
     7921 tatccgcgcg atggtgaagg atggtcgctt tgtggcggca aaaccgttcg aactggataa
     7981 atatccgtcg aaaatgatcg ccggattgcc ggatcacgta cacaacgcgg cacgtattcg
     8041 ttatccgatg gtacgcgtgg actggctgcg taagcgccat ctgagcgaca cctcccagcg
     8101 cggtgataac cgttttgtgc gcgtgagctg ggatgaagcc ctcgacatgt tctatgaaga
     8161 actggaacgc gtacagaaaa ctcacgggcc gagtgccttg ctgaccgcca gtggttggca
     8221 atcgacgggg atgttccata acgcttcggg gatgctggcg aaagctattg ccttgcatgg
     8281 taatagcgtt ggtacgggcg gagattactc taccggtgct gcgcaggtga tcctgccgcg
     8341 cgtagtcggt tcaatggaag tgtatgaaca gcaaacctcc tggccgctgg tattgcagaa
     8401 cagcaaaacc attgtgctgt ggggctccga tttgctgaaa aaccagcaag cgaactggtg
     8461 gtgcccggat cacgatgttt atgaatatta cgagcagcta aaagcgaaag tcgccgccgg
     8521 tgaagttgag gtcatcagca tcgatccggt tgtcacatcc acccatgagt atctggggcg
     8581 cgagcatgtg aagcacattg cggttaaccc gcaaactgac gtgccgctgc aactggcgct
     8641 ggcacatacg ctgtacagtg aaaacctgta cgacaaaaac ttcctcgcta actactgtgt
     8701 gggttttgag cagttcctgc cgtatctgct gggtgagaaa gacggtcagc cgaaagatgc
     8761 cgcatgggct gaaaaactga ccggcattga tgccgaaacc attcgtgggc tggcgcggca
     8821 gatggcggcg aacagaacgc aaattattgc tggctggtgc gtgcagcgta tgcagcacgg
     8881 tgaacagtgg gcgtggatga ttgtggttct ggcggcgatg ctggggcaaa ttggcctgcc
     8941 aggtggtggt tttggctggc actacaacgg cgcaggcacg ccggggcgta aaggcgttat
     9001 tctgagtggt ttctccggct ctacgtcgat tccgcctgtt cacgacaaca gtgactacaa
     9061 aggctacagc agcactattc cgattgcccg ttttatcgat gcgatcctcg aaccggggaa
     9121 agtgatcaac tggaacggta aatcggtaaa actgccgccg ctgaaaatgt gtatttttgc
     9181 cggaactaac ccattccatc gccatcagca gatcaaccgc attattgaag gctggcgcaa
     9241 gctggaaacg gttatcgcca tagataacca gtggacctca acctgccgct ttgccgatat
     9301 cgtgctgcct gcgaccacgc agtttgagcg taacgatctc gaccagtacg gcaaccactc
     9361 caaccgtggc attatcgcca tgaaacaggt ggtgccgccg tagttcgagg cgcgcaacga
     9421 ctttgatatt ttccgcgagc tgtgccgtcg ctttaatcgt gaagaagcct ttaccgaagg
     9481 gctggacgaa atgggctggc tgaaacgcat ctggcaggaa ggtgtacaac aaggcaaagg
     9541 acgcggcgtt catctgccag cgtttgatga cttctggaat aacaaagagt acgtcgagtt
     9601 tgaccatccg cagatgtttg ttcgccacca ggcattccgc gaagatccgg atctcgaacc
     9661 gctgggcacg ccgagtggcc tgattgagat ctactcgaaa accatcgccg atatgaacta
     9721 cgacgattgt caggggcatc cgatgtggtt tgagaaaatc gaacgctccc acggtgggcc
     9781 cggctcgcag aagtatccgt tgcatctgca atctgtgcat ccggatttcc gacttcactc
     9841 gcagctatgt gagtcggaaa cgctgcgtca gcaatatacg gtagcgggta aagagccagt
     9901 gttcattaac ccgcaggatg ccagcgcgcg cggtattcgt aacggtgatg tggtacgcgt
     9961 ctttaacgct cgcggtcagg tgttggcagg agcagtagtt tctgaccgct atgcacccgg
    10021 cgtggcgcga attcacgaag gggcatggta cgatccagat aaaggcggcg agcctggtgc
    10081 gctgtgcaaa tacggtaacc ccaacgtgct gaccatcgac atcgggactt cgcagctggc
    10141 gcaggcgacc agtgcgcaca ctacgctggt ggaaattgag aagtacaatg gaacagtgga
    10201 gcaggtgacg gcgtttaacg gccccgtgga gatggtggcg cagtgcgaat atgttcccgc
    10261 gtcgcaggtg aaatcatgac cacgctgaca gcacaacaga ttgcctgtgt ttacgcctgg
    10321 ctggcgcagt tgttctcccg tgaactggac gatgaacaac tgacgcaaat cgccagtgcg
    10381 cagatggctg aatggttttc gttgctgaaa agcgaaccgc cgctcactgc ggcggtgaat
    10441 gatctggaaa acagtattgc cacg
//
LOCUS       DAZPAB010000132        10460 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZPAB010000132 DAZPAB010000000
VERSION     DAZPAB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.132"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACHUPG_002921"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="ACHUPG_002921"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFR0862448.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /gene="mipA"
                     /locus_tag="ACHUPG_002922"
     CDS             1794..2540
                     /gene="mipA"
                     /locus_tag="ACHUPG_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR0862449.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="ACHUPG_002923"
     CDS             2630..3391
                     /locus_tag="ACHUPG_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR0862450.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="ACHUPG_002924"
     CDS             complement(3442..4326)
                     /locus_tag="ACHUPG_002924"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR0862451.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACHUPG_002925"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="ACHUPG_002925"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR0862452.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="ACHUPG_002926"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="ACHUPG_002926"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR0862453.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="ACHUPG_002927"
     CDS             6202..6474
                     /locus_tag="ACHUPG_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR0862454.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="ACHUPG_002928"
     CDS             6844..7920
                     /locus_tag="ACHUPG_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR0862455.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="ACHUPG_002929"
     CDS             7947..9326
                     /locus_tag="ACHUPG_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0862456.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="ACHUPG_002930"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="ACHUPG_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
BASE COUNT         2662 a         2415 c         2669 g         2714 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcgaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgctgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc caggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa ccgccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatttg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgactgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atgtggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gctccgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 taatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatagggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg
//
LOCUS       DAZPAB010000133        10417 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZPAB010000133 DAZPAB010000000
VERSION     DAZPAB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.133"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            2..1369
                     /gene="dcuC"
                     /locus_tag="ACHUPG_002931"
     CDS             2..1369
                     /gene="dcuC"
                     /locus_tag="ACHUPG_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR0862457.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1412..1909)
                     /gene="sspB"
                     /locus_tag="ACHUPG_002932"
     CDS             complement(1412..1909)
                     /gene="sspB"
                     /locus_tag="ACHUPG_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR0862458.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(1915..2553)
                     /gene="sspA"
                     /locus_tag="ACHUPG_002933"
     CDS             complement(1915..2553)
                     /gene="sspA"
                     /locus_tag="ACHUPG_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR0862459.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(2948..3340)
                     /gene="rpsI"
                     /locus_tag="ACHUPG_002934"
     CDS             complement(2948..3340)
                     /gene="rpsI"
                     /locus_tag="ACHUPG_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR0862460.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3356..3784)
                     /gene="rplM"
                     /locus_tag="ACHUPG_002935"
     CDS             complement(3356..3784)
                     /gene="rplM"
                     /locus_tag="ACHUPG_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR0862461.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4003..5130)
                     /gene="zapE"
                     /locus_tag="ACHUPG_002936"
     CDS             complement(4003..5130)
                     /gene="zapE"
                     /locus_tag="ACHUPG_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR0862462.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5324..5722
                     /gene="zapG"
                     /locus_tag="ACHUPG_002937"
     CDS             5324..5722
                     /gene="zapG"
                     /locus_tag="ACHUPG_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR0862463.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            5876..7223
                     /gene="degQ"
                     /locus_tag="ACHUPG_002938"
                     /pseudo
     CDS             5876..7223
                     /gene="degQ"
                     /locus_tag="ACHUPG_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            7313..8380
                     /gene="degS"
                     /locus_tag="ACHUPG_002939"
     CDS             7313..8380
                     /gene="degS"
                     /locus_tag="ACHUPG_002939"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR0862464.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8442..9380)
                     /gene="mdh"
                     /locus_tag="ACHUPG_002940"
     CDS             complement(8442..9380)
                     /gene="mdh"
                     /locus_tag="ACHUPG_002940"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR0862465.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            9815..10285
                     /gene="argR"
                     /locus_tag="ACHUPG_002941"
     CDS             9815..10285
                     /gene="argR"
                     /locus_tag="ACHUPG_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR0862466.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2546 a         2654 c         2611 g         2606 t
ORIGIN      
        1 aatgttcggc ataattatat ctgtcatcgt attaattacg atgggctatt tgatcctgaa
       61 aaactacaaa cctcaggtgg tgctggctgc cgcaggtgtc ttcctgatga tgtgcggagt
      121 ctggttaggg ttcggtggtg tactcgatcc cgccaaaagc agcggctact tgatcgtcga
      181 catttataat gaaatcctgc gcatgctgtc caaccgcatt gccggattgg ggctgtcgat
      241 tatggcggtg ggcggttatg cccgctacat ggagcgcata ggagccagtt gcgcgatggt
      301 gagcttgcta agccgcccgt taaaactcat tcgctcgccg tatattattc tgtcggcaac
      361 ttacgtcatc ggccaaatca tggcgcagtt tattaccagc gcctccggtc tgggtatgtt
      421 gctgatggtc accttatttc cgacgctggt gagtctggga gtaagtcgcc tctctgcggt
      481 ggcggttatc gcaaccacga tgtccattga gtgggggatt ctggaaacga actccatttt
      541 tgcagcccag gtcgcgggaa tgaaaattgc cacatacttc ttccactacc agcttccggt
      601 cgcctcttgc gtcattatct cggtggcgat ctcccacttt ttcgtgcaac gcgcttttga
      661 caaaaaagat aaaaatatca atcacgaaca ggcagagcaa aaaactctcg ataatgtccc
      721 gccgctctat tacgccattt tacctgtgat gccgttaatc ctgatgctcg gctcgctgtt
      781 cctcgcccac gtcgggctga tgcagtcaga actgcatctg gtggtggtga tgttactaag
      841 tttgacggtg acgatgtttg ttgagttctt ccgcaagcat aacttgcgcg aaacaatgga
      901 cgatgtgcag gcgttttttg acggcatggg tacgcagttt gtcaacgtgg taacgctggt
      961 ggtcgcgggt gaaatatttg cgaaaggctt aacgacgatt ggcactgttg atgcggttat
     1021 caggggtgcg gagcattctg gtctgggcgg tattggcgtg atgattatta tggcgctagt
     1081 cattgccatt tgtgccattg tgatgggctc tggcaatgcg ccgtttatgt catttgccag
     1141 tcttattccg aatatcgcag ccggactaca tgtaccagcg gttgtaatga ttatgccgat
     1201 gcattttgcc acgacgctag cgcgcgcggt ttcgccgatt actgcggtgg tggtcgttac
     1261 gtcaggaatt gcaggcgttt cgccttttgc ggtggtgaag cggacagcga tccccatggc
     1321 agtcggtttc gtggtgaata tgattgccac aatcacgcta ttttattaag tcattaaaaa
     1381 gacaaaacag gccgcctggg cctgttttgt attacttcac aacgcgtaat gccggtcgac
     1441 caccgcgtgg tggctgcgga ggttcatcgt caggatgagt gtcatcatcg tgatctggct
     1501 tgtcgccatc aataaccgac ataacggttt cgttgtctgc cgatgcctct tcatcattca
     1561 tgatgctggt atcttcatcg taggcagctt caggctcaaa catcgtgcct gcgccatttt
     1621 cacgggcgta gatagccagc acggcagcca gcggcacaga aacctgacgc ggaatgccac
     1681 caaagcgcgc gttaaagcgc acctcatcat tcgccagttc cagattgccg acagcacgcg
     1741 gcgcaatgtt gagtacgatt tgcccgtcac gcgcatattc cataggaacc tgcacgccag
     1801 ggagcgtcac atccaccacc aggtgcggcg tgagctggtt atccagcaac cactcataga
     1861 atgcacgcag cagataggga cgacgtggtg ttagctgtga caaatccata cagattaact
     1921 ccggcccaga cgcatttcac gttctgcttc agttaaagaa gcaaggaaag agtcacgctc
     1981 aaagacgcgg gtcatatagc ctttcagctc tttcgcaccc gggccgctga actcgatgcc
     2041 cagttgcggc agacgccaca gcagcggagc aaggtagcaa tcgaccaggc tgaactcatc
     2101 gctcaggaag tacggcttct gaccgaagac cggcgcaatc gccagcagtt cttcgcgcag
     2161 ttgcttacgt gcggcatctg cttcagaagc tgaaccgttg atgatagtgt tcatcagcgt
     2221 gtaccagtct ttttcgatgc gatgcatgta cagacggctt tcaccgcgag ctaccgggta
     2281 aacaggcatc agtggcggat gcgggaaacg ctcatccaga tattccataa tgatgcgaga
     2341 ttcccacagg gtcagctcac gatccaccag ggtcggaacg ctctgattcg ggttgaggtc
     2401 aatcagatcc tgaggcggat tgtccttttc cacgtgttcg atctcgaaac ttacaccttt
     2461 ctcagccagc acaatgcgga cctgatggct atagatgtca gtaggaccgg aaaacagcgt
     2521 cattaccgaa cgtttgttgg cagcgacagc catgaaaacc tccaggtata gtcagaattt
     2581 ttactgctac cagccaccag gtggccagtc agaagttgtg ttacccaata aggaacgact
     2641 ctctttgttc gaaaatcaaa caaaaaatga gcaatacccg acatttgggc agaaaattgg
     2701 atgatagttt accagatttt gcgacctttg tggtgagtgg atgccggaaa tggggaaaaa
     2761 gagatgcgct ttagtctgaa atagttgact tagaccctta ttggcgatgt ggtttttgtt
     2821 ttacctgtct ttcagacggc agcaaaaagc aacttcccat tttttacgct gattcagaat
     2881 ttagccataa aaaaacccgc cgaagcgggt tttttcgcaa attgttttct gccgtagcag
     2941 aagccaatta acgtttggag aactgcggac gacgacgtgc tttacgcaga ccgactttct
     3001 tacgttcaac ctgacgagcg tcacgagtaa cgaagccagc tttacgcagt tcagaacgca
     3061 gggactcgtc gtattccatc agagcgcggg tgataccgtg acggatcgca ccagcctgac
     3121 cagagatacc accaccttta acggtgatgt acaggtccag tttctcaacc atgtcgacca
     3181 gttccagcgg ctgacgaact accatgcggg cagtttcacg accgaagtac tgttccagag
     3241 aacgttggtt gattacgatt ttaccgttgc ccggtttgat gaaaacgcga gctgcggaac
     3301 ttttgcggcg accagtgccg tagtattgat tttcagccat tgcctataat cccgattaga
     3361 tgtcaagaac ttgcggttgc tgtgccgcgt ggttgtgctc gttacccgcg taaactttca
     3421 gtttacggaa catagcacga cccagcgggc cttttggcaa catgccttta accgcgattt
     3481 caatcacacg ctcaggacgg cgagcaatca tctcttcaaa ggtcgcttgt ttgataccac
     3541 cgatgtggcc ggtgtggtga tagtacactt tgtcagtacg cttgttgccg gttacagcaa
     3601 ctttgtcagc gttcagaacg atgatgtaat caccggtatc tacgtgcgga gtatattccg
     3661 ctttgtgctt accgcgcagg cgacgagcca gttcagtagc cagacggccc agagttttac
     3721 cggtcgcgtc aacaacatac cagtcgcgtt ttacggtttc tggtttagct gtaaaagttt
     3781 tcattaaaag cttacccaat aaatagttac acgttggtga acacccaaac gtcttcaatt
     3841 gttgaggttc acacgacaaa gtccggcaaa cctacccctt cgaatagcct atgccagcac
     3901 acaaaaagtt ttgggaaaaa aactttcttg taacgtgggg tcgcaggatt atagagaagt
     3961 cggggtcaaa gatcgacccc tttttgtgat ttgtggcagg ttttaacccg ccaaatgctc
     4021 gcgcttcaga tactcttcgc tttgcatctc ttgcagacgt gacaggcaac gctggaactc
     4081 aaacttcagc cgctcaccct gataaatttc atacagcggc acttctgcac tcaccactaa
     4141 tttgacatgg cgctcgtaaa actcatccac cagcgcaata aagcgccgcg cttcgctctc
     4201 catcaaccgc gtcatgactg gcacatcaaa caacattact gtgtgaaaca ggcgtgagag
     4261 cgcaatatag tcatgctgac tgcgggcgtc gacgcacagc gtagtaaaag aaaccgccag
     4321 cgtctggttc tcgacgccca ttgtcgccaa tggccgatgg ttgatttcta acgtcggtga
     4381 attttctcgt ttcacccccg ccagcgccaa ccatagttta tccatctgcg cccgggtttc
     4441 atcgttgagt ggcgaaagcc acagatgcgc ctgagtgagt gtacgcagtc gataatcaac
     4501 accagcgtcc acgttcatta catcacaatg ctgtttaatg gcatcgattg caggcagaaa
     4561 acgcgcacgt tgcaggccat ttcgataaag ttcgtccggc ggaatatttg acgtcgctac
     4621 cagggtaata ccgcgggtga acagggcttt catcagaccg ccaagtagca tggcgtcggt
     4681 aatatcagaa acaaaaaatt cgtcaaaaca gagcacgtca gtttcggctt taaagcgatc
     4741 ggcaataatt tccagcggat cggtctgccc ctgtaaggca gttagctctt cgtgcacccg
     4801 cagcataaaa cggtgaaagt gcaggcgctg tttccgctct cccggcaggc tttgatagaa
     4861 aaggtccatc agccaggttt tcccgcgtcc tacaccgccc cacatatata agccacgcac
     4921 tggcgtatgc tttgtgtctt cgcgtttacc ccacagctta ccgacccgcg ccattagccc
     4981 actcgtcctg ggggctggtg gcctgctatt gatgagttct tgataaataa tttccaggcg
     5041 gctgacggcc tctttttgaa cgtcgtcggg ttgatggctg ccttcattga gcgccttcag
     5101 gtattgcgat gttggggtaa cgctttgcat gatcttattg ttattccttg aataatcggt
     5161 gcgccgttgt tcacggttga cgaaaaaaag gccgttctac actacgcgat atgcagtcgg
     5221 gattccactt ctgtggaatt aacggttata gtggcataat cagccgcagg catggagcct
     5281 gaagccaaca ccctacggaa acaaaagaca acgggagatg ttcatgacct gggaatatgc
     5341 gctaattggg ttagtcgtcg gcatcattat tggtgctgtg gccatgcgtt ttggtaatcg
     5401 taaactacgc cagcaacagg cgttgcagta cgaactggaa aagaataaag ctgaactgga
     5461 cgagtatcgc gaagagctgg ttagccactt tgcccgcagc gcggaattac tggataccat
     5521 ggcgcacgac tatcgccagc tgtatcagca catggcaaaa agctccagca gcctgctgcc
     5581 ggaactgtct gctgaagcaa acccgttccg taatcgtctg gcagagtctg aagccagcaa
     5641 cgatcaggca ccggtgcagg tgcctcgcga ctattctgaa ggcgcatccg gcctgctgcg
     5701 tactggcgcg aagcgcgact aatttatttt tcgggcgcag ccattgcgcc ctcctcttct
     5761 ctccctcccc gactatcatt taatctggtg tctcattgtt agccgtctga aaattcaata
     5821 acatcaaact gttttgaatc tcttttctta tcattcaggt acgagagcag gaataatgaa
     5881 aaaacaaacc cagctgttga gtgcattagc gttaagtgcc gggttaactc tctcggcgtc
     5941 atttcaggcc gtcgcgtcga ttccaggcca ggttgccgat caggcccctc tccccagtct
     6001 ggctccaatg ctggaaaaag tgcttccggc agtggtgagc gtacgggtgg aaggaacggc
     6061 cagtcaggga cagaaaatcc cggaagaatt caaaaagttt tttggtgatg atttaccgga
     6121 tcaacctgca caacccttcg aaggtttagg ttccggtgtc atcatcaacg ccagtaaagg
     6181 ctatgtgctg accaacaacc atgtgattaa tcaggcacag aaaatcagta ttcagctcaa
     6241 tgatgggcgc gagtttgatg caaaactgat tggtagcgat gaccagagcg atatcgccct
     6301 gttacaaatt caaaacccga gcaaattaac gcaaatcgct attgccgact ccgataaatt
     6361 gcgcgtcggt gattttgccg tagcggtcgg taacccattt ggccttgggc aaaccgccac
     6421 ctctggcatt gtttccgcat taggccgcag cgggttgaat cttgaaggtc tggaaaactt
     6481 tatccagaca gatgcttcca ttaaccgcgg taactccggc ggtgcactat taaaccttaa
     6541 cggtgagtta attggcatca acactgcaat ccttgcgcct ggcggcggga gcgtcgggat
     6601 tggatttgcc atccccagta atatggcgcg aacactggcg cagcagctta tcgactttgg
     6661 tgaaatcaaa cgcggtttgt taggcatcaa aggcaccgag atgagtgccg atatcgccaa
     6721 agccttcaac cttgacgtgc agcgtggcgc gtttgtcagc gaagtgttgc caagttctgg
     6781 ctcagcgaaa gcgggcgtca aagcgggcga tattattacc agcctcaacg gcaaaccgct
     6841 gaatagcttt gctgagttgc gctctcgtat cgcgactaca gagccgggca cgaaagtgaa
     6901 gcttggcctg ctgcgtaacg gcaaaccact ggaagtagaa gtgacgcttg ataccagcac
     6961 ctcttcgtcg gccagcgctg gaaggtgcaa cgttgagcga tggtcagcta aaagatggcg
     7021 gcaaaggtat taagatagat gaagttgtca aaggaagccc agctgctcag gctggcttgc
     7081 aaaaagacga tgtgatcatt ggcgtcaacc gcgatcgggt gaactcgatt gctgaaatgc
     7141 gtaaagtgct ggcggcaaaa ccggccatca tcgccctgca aattgtacgc ggcaatgaaa
     7201 gcatctatct gctgatgcgt taatgtcgta aaccgggcat caggctgacg tgtgatgtcc
     7261 ggttaactcg tggtatgctg ctgccgttcc cttttttaat gacgcctcca tcatgtttgt
     7321 gaagctctta cgttccgttg cgattggatt aattgtcggc gctattctgc tggttgccat
     7381 gccttcgctg cgcagcctta acccgctttc cactccgcaa tttgacagta ccgatgagac
     7441 gcctgccagc tataatctgg cggttcgccg cgccgcgcca gcggtggtta acgtttacaa
     7501 ccgtggtttg aacaccaact ctcacaacca gcttgagatc cgcaccctgg gatccggtgt
     7561 aatcatggat caacgcggtt atatcatcac caataaacac gtcatcaacg acgccgatca
     7621 gatcatcgtc gccttacagg atggacgtgt atttgaagca ttgctggtgg gatctgactc
     7681 tctaaccgat ctggcggtac ttaaaattaa tgccactggc ggtttaccta ccattccaat
     7741 taatgcacgt cgcgtaccgc acattggcga cgtagtactg gcgatcggta acccgtacaa
     7801 cctcgggcag accattaccc aagggattat tagtgccacg ggtcgaatcg gtctgaaccc
     7861 gaccgggcgg caaaacttcc tacaaaccga tgcttccatt aaccacggta actctggcgg
     7921 cgcgctggtg aactcgctgg gagaactgat gggcattaac acgctgtcgt ttgataagag
     7981 taacgatggc gaaacgccgg aaggtatcgg ctttgcgatt cctttccagt tagcaaccaa
     8041 aattatggat aagctgatcc gcgatggtcg cgtgatccgc ggctacattg gtatcggcgg
     8101 acgcgagatc gcaccactgc acgcgcagag cggtggtata gatcaactgc aagggatcgt
     8161 ggttaatgaa gtgtcacctg acggcccagc ggcgaatgcg ggtattcagg tcaacgatct
     8221 gattatttcg gtggataaca aaccggccat ctctgctctg gagacgatgg atcaggtggc
     8281 ggaaattcgc cctggttcgg tgatcccggt tgtagtgatg cgtgatgata agcagttaac
     8341 gctgcaggtc accattcagg aatatccggc aaccaattaa gtcgagtgct caaaacaaaa
     8401 accggagtct gtgctccggt tttttattat tcgctaatca attacttatt aacgaactct
     8461 ttgcccaggg cgatatcttt cttgagcgta tccagcatac cttccagcgc gttctgttca
     8521 aatgcgctca gggtaccgat agatttacgc tcttccacgc cgtttttacc cagcagcagc
     8581 ggttgagaga agaaacgggc gtactgaccg tcgccttcaa cgtaggcaca ttcgacaacg
     8641 ccttgttcgc cctgcagtgc acgaaccaga gacagaccaa aacgtgcagc tgcctggccc
     8701 atagacaggg ttgcagaccc gccaccggct ttcgcttcaa ccacctcagt acccgcgttc
     8761 tggatacgtt tggtcagatc agccacttcc tgctcggtaa aactaacgcc aggaacctgt
     8821 gacagcagcg gcagaatggt aacaccagag tgaccgccaa taaccggcac ttcaacttcg
     8881 cctggctgtt tgcctttcag ttccgcaaca aaggtgttgg aacgaatgat atccagcgtg
     8941 gtaacgccga acagtttgtt tttgtcataa acaccggctt ttttcagcac ttcagcagca
     9001 attgcaactg tggtgttaac cgggttagtg ataataccaa tgcacgcttt cgggcagttt
     9061 ttcgcaactt gctgtaccag gtttttcacg atgccggcgt taacgttaaa caggtcggaa
     9121 cgatccatac ccggtttacg cgctacgcct gcagagataa gaacgacatc tgcgccttcc
     9181 agcgccggag tcgcatcttc accagaaaaa cctttgattt tcacagcagt agggatatgg
     9241 ctcagatcga cggccacacc gggagtcact ggagcgatat catacagaga gagttctgaa
     9301 cctgaaggca gttgggtttt taacagtagt gcaagcgcct ggccaatacc gccagcagcg
     9361 ccgaggactg cgactttcat cctaaactcc ttattatatt gataaactaa gatatgttgc
     9421 tccgctgccg cgaccttaat ccacaaaatt gccgtttaca atgaccacat ctcaagaatg
     9481 tgtagtcacg caagtttagc gtttatgcat ttaattgccg taatcaggaa cctaacttac
     9541 gtaattaaca gtcgcttatg cattagcgca acattccaac aggtggtgac aatataccct
     9601 accgttcagc caaaacaaca tcaatttgat aacaattaat ttacttttaa acagaatttg
     9661 catgccgtga cgcaggcatg tttctcaata acgaaatttg ataaaatccc gctctttcat
     9721 aacattattt cagccttctt cagggctgac tgtttgcata aaaattcatc tgtatgcaca
     9781 ataatgttgt atcaaccacc atatcgggtg acttatgcga agctcggcta agcaagaaga
     9841 actagttaaa gcatttaaag cattacttaa agaagagaaa tttagctccc agggcgaaat
     9901 cgtcgccgcg ttgcaggagc aaggctttga caatattaat cagtctaaag tctcgcggat
     9961 gttgaccaag tttggtgctg tacgtacacg caatgccaaa atggaaatgg tttactgcct
    10021 gccagctgaa ctgggtgtac caaccacctc cagtccattg aagaatctgg tgctggatat
    10081 cgactacaac gatgcagttg tcgtgattca taccagccct ggcgcggcgc agttaattgc
    10141 tcgcctgctg gactcactgg gcaaagcaga aggtattctg ggcaccatcg ctggcgatga
    10201 caccatcttt accacccctg ctaacggttt caccgtcaaa gacctgtacg aagcgatttt
    10261 agagctgttc gaccaggagc tttaatctct gccccgtcgc ttctggcggc ggggaaaatg
    10321 ttgcttatcc ctctcattcc ccttctttct cctgcgatac atttaacgaa tagtgcgttt
    10381 taccgcgaca catcattcac aaaatgaata catacgg
//
LOCUS       DAZPAB010000134        10299 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZPAB010000134 DAZPAB010000000
VERSION     DAZPAB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.134"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(1..71)
                     /locus_tag="ACHUPG_002942"
                     /pseudo
     CDS             complement(1..71)
                     /locus_tag="ACHUPG_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            125..2099
                     /gene="pdeR"
                     /locus_tag="ACHUPG_002943"
                     /pseudo
     CDS             125..2099
                     /gene="pdeR"
                     /locus_tag="ACHUPG_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(2081..2209)
                     /gene="ymiD"
                     /locus_tag="ACHUPG_002944"
     CDS             complement(2081..2209)
                     /gene="ymiD"
                     /locus_tag="ACHUPG_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR0862467.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            2247..2420
                     /gene="yciZ"
                     /locus_tag="ACHUPG_002945"
     CDS             2247..2420
                     /gene="yciZ"
                     /locus_tag="ACHUPG_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR0862468.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            2510..3259
                     /locus_tag="ACHUPG_002946"
     CDS             2510..3259
                     /locus_tag="ACHUPG_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR0862469.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            3528..3746
                     /gene="osmB"
                     /locus_tag="ACHUPG_002947"
     CDS             3528..3746
                     /gene="osmB"
                     /locus_tag="ACHUPG_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR0862470.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(3872..4198)
                     /gene="yciH"
                     /locus_tag="ACHUPG_002948"
     CDS             complement(3872..4198)
                     /gene="yciH"
                     /locus_tag="ACHUPG_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR0862471.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(4198..4935)
                     /gene="pyrF"
                     /locus_tag="ACHUPG_002949"
     CDS             complement(4198..4935)
                     /gene="pyrF"
                     /locus_tag="ACHUPG_002949"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR0862472.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(5128..6297)
                     /gene="lapB"
                     /locus_tag="ACHUPG_002950"
     CDS             complement(5128..6297)
                     /gene="lapB"
                     /locus_tag="ACHUPG_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR0862473.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(6304..6612)
                     /gene="lapA"
                     /locus_tag="ACHUPG_002951"
     CDS             complement(6304..6612)
                     /gene="lapA"
                     /locus_tag="ACHUPG_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR0862474.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(6761..7525)
                     /gene="pgpB"
                     /locus_tag="ACHUPG_002952"
     CDS             complement(6761..7525)
                     /gene="pgpB"
                     /locus_tag="ACHUPG_002952"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR0862475.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            7695..8285
                     /gene="ribA"
                     /locus_tag="ACHUPG_002953"
     CDS             7695..8285
                     /gene="ribA"
                     /locus_tag="ACHUPG_002953"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR0862476.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(8349..>10299)
                     /gene="acnA"
                     /locus_tag="ACHUPG_002954"
     CDS             complement(8349..>10299)
                     /gene="acnA"
                     /locus_tag="ACHUPG_002954"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR0862477.1"
                     /translation="SMFIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVGKFVE
                     FYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELVEKYA
                     KAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNELKVN
                     ATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVTLGLK
                     RQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDPIETA
                     IKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLASEPIGHD
                     RKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDTYGWQ
                     EDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSPAGRY
                     LQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSDVVSI
                     YDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSNLIG
                     MGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVPCRCR
                     IDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT         2626 a         2623 c         2428 g         2622 t
ORIGIN      
        1 ccaaaactgg attacaaata aatatataaa aaaacaatgc gtttagccgc cgttaatttc
       61 atgtaagtca tgttgttcct tttatttaac cgcggactcc gctgttaacc ggaggatatg
      121 catcatgaaa accgttaggg agtccacaac gttgtacaac tttctcggat cgcacaatcc
      181 atactggcgg ttgacggaaa gcagcgatgc tttgcgcttt tctaccacca aaaccacaga
      241 acctgatcgt acattgcagt tatctgccga acaggctgct cgcatcaggg aaatgacggt
      301 catcacctcc agcctgatga tgagtctgac cgtcgatgaa agcgatcttt ctgtgcatct
      361 ggtaggacga aaaatcaata aacgggaatg ggctggcaac gcgtctgcat ggcatgacac
      421 accggcggtt gctcgtgatt tatcacacgg gctttccttt gctgagcagg tagtttctga
      481 agcacattcc gcaatagtga ttctcgacag ccgggggaat atccaacgct tcaatcggtt
      541 atgtgaagat tacacagggt tgaaagaaca cgacgtcatt gggtaaagcg tgtttaaact
      601 gtttatgagc cgtcgtgaag ctgcggcatc caggcgcaat aaccgtgtat tttttcgaag
      661 cggcaatgca tatgaagtcg aactgtggat accaacacgt aaaggccagc ggctgtttct
      721 gtttcgcaat aaatttgtcc acagcggcag tggcaaaaac gagatttttt taatctgttc
      781 cggtaccgac attaccgaag agcgccgcac tcaggagcga ctgcgtattc tggcaaatac
      841 cgacagtatc accggactgc cgaatcgtaa cgcaatgcag gagttaatcg atcacgctat
      901 taatcaggca gataacaata aagttggggt tgtgtatctt gatttggata attttaaaaa
      961 ggtcaacgac gcctatgggc atttgtttgg tgaccagtta ttacgcgacg tgtcattggc
     1021 tattttaagc tgtctcgaac atgaccaggt gttggcgcgt ccaggtggcg atgagtttct
     1081 ggtactggca tccaatacct cacaaagcgc gctggaagca atggcatcac gaattttgac
     1141 ccgcttacgc ctcccctttc gtattggttt aattgaagtt tataccagct gttcagtagg
     1201 tatttcgctc tctcccgaac atggttcgga cagcgcgact attattcgtc acgccgacac
     1261 agcaatgtac acagcgaagg aaggcggacg aggacaattt tgcgttttta ctccagaaat
     1321 gaatcagcgg gtatttgaat atctctggct ggataccaac ttgcgtaaag cactggaaaa
     1381 cgatcagttg gttatcgaaa atcacctagc gtgtcgaagt gcgcagtctg gaagcactag
     1441 tacgttggca gtcacctgaa cgtgggttga ttccaccgtt ggacttcatt tcctacgccg
     1501 aagagtcagg gctaattgtg cctttaggcc gttgggtgat tctcgatgtc gtacgccagg
     1561 tggcaaagtg gcgggataaa ggcataaacc tgcgagtggc ggtaaatatt tctgcacgtc
     1621 agctcgccga tcaaaccatt ttcaccgccc tgaaacaggt tctccaggaa ctcaattttg
     1681 aatactgccc tatagatgtt gaactgacag agagttgtct gattgagaat gatgaactgg
     1741 cactgtctgt tattcaacaa tttagccaac taggtgcgca agtgcatctg gatgattttg
     1801 gtaccggcta ctcttcactt tcgcaactgg cgcgctttcc gatcgatgcc atcaaacttg
     1861 accaggtttt tgttcgagat attcacaaac aacctgtctc gcagtcactg gtccgggcga
     1921 tcgtcgctgt ggcccaggca ttgaatcttc aggtgatcgc cgaaggtgta gagagtgcaa
     1981 aggaagatgc ttttttaacc aagaacggga tcaatgagcg gcaaggattt ttgtttgcca
     2041 aaccgatgcc cgccgtcgcc ttcgaacgct ggtataaacg ctatctgaag cgcgcataag
     2101 tagatgaata tatatactta gcgctatatc ccgtcattat atatcgtatt cctggatgat
     2161 aattagccgt tgctgttaaa cacttctctt ttagcggcaa tttttgcatt attcattttc
     2221 ccccttttgc tataaggatg tccttgatgt ctgaatttga tgcccagcgc gttgccgaac
     2281 gtattgatat tgtgttagat atccttgtgg caggcgatta ccactctgcg atccataatc
     2341 tcgagatact taaagcagaa ctcttgcgcc aggttgctga atctacgcca gacatcccga
     2401 agacaccgtg ggagatttaa caatcgtgcc ttcaggtttt cctatttgag gatgcccggc
     2461 gcgtcagtat ttttttattt agtattataa cgttataaga attacagcga tgaattcccg
     2521 acaacaaact attctacaga tggtcattga ccagggtcag gttagcgtaa ccgatctggc
     2581 aaaagccact ggagtttctg aagttaccat tcgccaggat ctcaacaccc tcgaaaaact
     2641 gagttacctc cgccgtgcac atggctttgc agtttcgctt gatagtgatg acgtcgaaac
     2701 ccgtatgatg agcaactata cgctgaagcg tgaactcgcc gagtttgccg cgtcactggt
     2761 tcaaccgggc gaaaccatct ttatcgaaaa tggcagcagc aatgccctgc tcgcccggac
     2821 tctgggggag cagaagaaaa atgtcactat catcacggtc agcagctaca tcgcgcattt
     2881 gctgaaagac gcgccttgtg aagtcatttt gctcggtggc gtgtaccaga aaaaaagcga
     2941 aagtatggtt ggccctttga cacgccagtg catccagcag gtacatttca gcaaagcatt
     3001 tattggtatt gatggctggc aacctgaaac tggattcacc ggtcgcgata tgatgcgcac
     3061 cgatgtggtc aatgccgtgc tggaaaaaga gtgcgaagcg atagtcctga ctgacagctc
     3121 gaaatttggt gctgtacatt catactccat cggtcccgtt gagcgattca atcgcgtgat
     3181 taccgattcg aaaatacgcg ccagcgatct gatgcatctt gagcacagta aactcaccgt
     3241 tcacgtcgtt gacatttaac catttccctt ctctacggat gatttgcagt ttggcaaatc
     3301 atccgctcta agatgattcc tggttgataa ttaagactat ttacctgtta ttaacactct
     3361 caagatataa aattattatc agcgatataa caggaagtaa ttatcacctg cgtgatataa
     3421 ccctgcgcgc gagcagattt cacggaataa tttcaccagg cttattctta gctattatag
     3481 ttatagagag cttacttccg tgaatcataa attcaggaga gagtattatg tttgtaacga
     3541 gcaaaaaaat gaccgcggct gttctggcaa ttactttggc aatgtctctg agtgcctgtt
     3601 ctaactggtc taaacgggac cgcaacaccg caatcggcgc gggtgcaggg gcattaggcg
     3661 gtgcagtact gaccgatggc agtacgttgg gtacattagg tggtgcagct gtcggtggtg
     3721 ttattggtca ccaggtcggt aaataatcgt aaataaatgt cgattatcga ctcgcaatta
     3781 taatacgctt gattctgttc agtacgaaat aactgaataa tacacacagt aaaatattcg
     3841 aaagccacgg atatatccgt ggctttttat gttaaccgcc tgcgagtttt accttcatcc
     3901 ctttcgcttc aagtagtgat tttaataaat cacgcttatc gccctggatt tcaataactc
     3961 catctttaac tgctccgccg cagccgcatt ttttcttcag ttccgctgca agttttgtca
     4021 gttcggcatc atcgagatcg ataccggtaa tcaggcaaac gcccttacct ttacgtccac
     4081 tggtctgacg ctgaatacgc accgctccat cgcctttagg gcgtactggg gccgctttgg
     4141 gctcatcaat acgcccggtt tccgttgagt agaccagacg gctgttggaa tcactcatca
     4201 tgcactccgc tgtaaagagg cgttgatcgc tttcagtgtc tgcgctggat ctaccgattg
     4261 cgttaccggg cgaccaatca ccatataatc aacaccagcc gccaacgcct gttctggcgt
     4321 cataatgcgg cgctggtcac cagcttcact cccctgcgga cgaatgcccg gtgtaaccag
     4381 tttgaactcc tgaccgaata cctgtttaaa gcgcacagct tcctgagcag aacacaccac
     4441 accatcaagg tcacattttt gcgtcagtgc tgccagacgt tctgcataat ctgcaggtga
     4501 cagtgtcacg ccaagatcgg ccaggtcgct ggcttccatg ctggtcaaca ctgtcacagc
     4561 aatcaaaagc ggtgcatctt tgccaaacgg aaccagtgcc tcacgcgctg cggtcatcat
     4621 acgcgcccca ccggaggcat gaacattcac catccacacg cctaagtcag ctgcagcagc
     4681 gacagcgtgc gctgcagtgt tggggatatc gtggaatttc aggtcaagaa agatatcaaa
     4741 accacgctgt tgaagttcgc gcacaaactg tggcccaaac aatgtaaaca tctctttacc
     4801 gaccttcaga cgacaatcgc gtgggtcgat cttgtcgaca aaggacagcg cgtcatcaca
     4861 attatgataa tcaagggcaa caaccacagg agaattcgta acggcgcggg aagaagatga
     4921 agcagttaac gtcatgacca gaccttcttg atgatgggca ccgggagtgg cgcgaaacag
     4981 gtaaacggcg agcattctac ccgtgaaaat aaaaaattaa cagttgcgat ttttccccta
     5041 gcaggctttt tctgctccgt cgttgccgga gcacaatggg acatcaataa ctagtatgtt
     5101 gtaactaaag tcggattttt ttaaaaatta caggccatca agaccgcgaa tcggtttaat
     5161 agttgaccag gcacgacaag acggacaatg ccagtagagg gtgtatgcgg taaaaccaca
     5221 tttctggcag ctataacgag gcttactacg caccttctcg ccaaccatgt cacgcagcac
     5281 catcagactc tctttggcgc gcccttcttc cgcttcattt aagtggtaat ccattagctt
     5341 atggaataca cgcatggtcg gatgacgctg aagctggcgt gtaatataga cctgtgcagc
     5401 ctcactaccg tcgcgcgctt cgatgatatc agcaagcatc aattcagcat cggcaccggt
     5461 gttctcttcc accgcgcgtt gcaggaattc tgcccattcg gcagttttac ccaactgctg
     5521 atagcaggtt tgcaacattt ccagcgtttc gctgaccagt tctctgtcct gggatatgac
     5581 gcgttgcaaa ctttcgacgg ctttggcgta ttctcctttc gccataaaca cgcgtcccat
     5641 catgatggat acgcgggcgc tgtttttatc tgccgccgcc ccttttttta gcaaggtcat
     5701 ggcacgatcg agatcgtcgc tggccatatg ctgcagggct aactcacagt agaaatgggc
     5761 aatttcgacg cgctgtttat ctttacccag cttcaccagg cgttcggcaa catcaattgc
     5821 tttctgccac tcactggtag cctggtagat ttgtagcaac tgttgcagcg cgccaatgcg
     5881 gaagtcagtt tcatcggtca gctgattgaa catgtcttcc gcgcggtcgt ataacccggc
     5941 ggccatgtaa tcacgcccca gttgttgaat cgccaacaga cgctgttcat aggtcagcga
     6001 ggcgctttcc attagggtct gatggatgcg aatagcgcga tcaacttcgc cacgcgaacg
     6061 gaacaggttt ccgagcgtaa ggtgggcttc aacggtgcct gtatcctctt taagcatatc
     6121 gagaaacagg tctaccgctt tatcctgttg attactaagc aggaagttaa cccccgctac
     6181 gtaatcacgc gacaagcggt tggcttcatc ttgcttgttt tgttgcgcac ttctgcggcc
     6241 catataccag ccataggcag cggctacagg caaaagcaga aacaacaact ccagcataga
     6301 aagttattcc ttcgccgctg acgagtgcgg cactacagcc acgtcagtcg cgggtgaaag
     6361 ctggttttcc agtcgcttta ttttacgttc agcgcgcgcc agggaaacac gaactcgcag
     6421 ccagaacagg ccacaaatca accaaccgat agcaaacccc gcagcaaaca ataccgccag
     6481 caatgtggaa atacggtact ccccttgcgc taacagataa ttaaacgtca cctgttgatc
     6541 gttctgcgca cccaacgtga ccgaaatcac gaagatcgct aacaccagta agaaaatgag
     6601 taaatatttc acattacttc ccgttatgtg gttagagcga ataaattatg gtcaacttac
     6661 cgcaatagcc ctttaaagta ccattttcgc gccgtctgcg aaatggcatc cacgcgcatt
     6721 gttgatgatt taaggcctaa attcggaaaa tcaaccagcg ttaactttct tgttctcgtt
     6781 gtgcaatttc gcgattttct tccgcaggtg gtgttaatgg cccacaaatt cgttgcgcaa
     6841 gccacgttgc caccgccacc agcgcccacg aaatcaacgt agctaccacc agatcgcgtg
     6901 gccaatgcat cccgagcagc aggcggcttc ccatgactcc cgttgcccag accaacaaga
     6961 tagcaatggt taacgttcgc cgacgcggcc acagcaaacc aacggccagc agtgcccaac
     7021 tggcagcaaa catcgtgtga ccggaaggaa aggcaaaccc cgtctctttc tgccagtgtg
     7081 aacgcaaata ttgtggaata tttttctctt cagccaactg ttcttttact agatttccgc
     7141 gttctgctcg ctttaaagtg tagaactcat caaccggaat atgatgtgtt ttttccaacc
     7201 agataacaaa aggtcgtggt tcctgtactt tgtctttgat ccaggattta acgccttgtc
     7261 caacaaggat tgccgccgcc agaatggcaa ataatacaaa ggcagcctta atgcgaaaac
     7321 gcagacacca gagaaaccag ccgaacaaaa tcaaatgtgt aatgacgccc cagggctggg
     7381 tgacagtttc agtaacccaa aaagccgctt ttagtagcca actttgttct ccaggttgcc
     7441 aacgccagcc agaaatccat acggctactg gcatgacaag caatagtgca gctcccactg
     7501 cggtacgtcg ggcaatcgaa cgcatggcct ctcctttttg ataagtccca caatcataac
     7561 tgaaaatgcc agttccaaga aaaattgaca gatttgtgcc attccgtgaa cgatcgacgc
     7621 gtcgtgatta ggtgaacccc ttctcgttat ggcaaaataa gccaatacag aaccagcatt
     7681 atctggagaa tttcatgcag cttaaacgtg tggcagaagc caaactgcca accccatggg
     7741 gcgatttcct gatggtggga tttgaagaac tggcaaccgg acacgatcat gttgcgctag
     7801 tctatggcga tatttccggg cataccccgg tacttgcgcg cgtccattcc gaatgtctga
     7861 ccggtgacgc cctgttcagc ttgcgctgcg attgtggctt ccagctcgaa gcggcattga
     7921 cgcaaattgc cgaggaaggc cgtggtattt tgctgtatca ccgtcaggaa ggtcgtaaca
     7981 ttggtctgct gaataaaatc cgcgcttacg cactgcagga tcaaggttac gataccgtag
     8041 aggctaacca ccagttaggc ttcgccgctg atgagcgcga cttcactctt tgcgctgata
     8101 tgttcaaact ccttggcgtc aatgaagtcc gcttgttaac caataacccg aaaaaagtcg
     8161 aaattctgac cgaagcaggg attaatattg ttgaacgcgt accattgatt gtaggtcgta
     8221 accccaataa cgaacattat ctcgatacca aagccgagaa aatgggccat ttgctgaaca
     8281 aataaccctc ttgcatcgtg taattcattt gcttgccgga agcaaaataa ccggcaagca
     8341 aatacttgtt acttcaacat attacgaatg acataatgca aaatgccgtc gttctggtag
     8401 taggtcaact ccgtcgcggt gtcgatacga caacggcagg gtacgacttc ctggctacca
     8461 tccgcgcgcg taagcgtcac cggaaccgtc gcgccaggtt gtagattttg cagatcgcca
     8521 atatcaatct tctcttcccc ggttagccct aacgttttac gcgtcacgcc ttgcggaaat
     8581 tccagcggca ggatgcccat gccaattaaa ttcgaacggt gaattcgttc aaacgattcg
     8641 gcaatcacca cacgaattcc aagcagacga ggtccttttg ccgcccagtc acggcttgag
     8701 cctgatccat actctttccc ggcaatcacc gccagcggcg tttgctcctg cttatagcgc
     8761 atcgcagcat cataaataga gactacgtcg ctgtcaggta aatgccgcgt catcccccct
     8821 tcaacgccag gcaccatttc attacggatg cgaatattgg cgaaggtgcc gcgcatcatc
     8881 acttcatggt taccacgccg cgaaccgtag gagttaaagt cttttcgctc aacaccccga
     8941 ccttgtagat atcgacccgc tgggctgtcg ggcttaatac tgcccgccgg agagatatgg
     9001 tcagtggtga ctgaatcccc cagcattgcg aggatccgcg caccgtgaat atcttccact
     9061 ggtgctggtg ttgcctgcat ttcatcaaag aaaggcgata agcgaatata ggttgagtcc
     9121 tcctgccaac cgtaggtatc ggatcgtgtg acgttaattt ccttccactc tgctgtgcct
     9181 tcaaaaactt ctgcgtactc tttgcggaac atttctgtgg agacttgttc taccgcacgg
     9241 gcaatttctt gtgccgatgg ccagatatct ttcagataaa ccggctcgcc tttgcgatca
     9301 tggccgatag gctcagaagc caggttgata ttcatatttc ccgccagcgc ataggcaacc
     9361 accagcggtg gcgaggccag ccagttagtt ttaaccagcg gatggatacg gccttcaaag
     9421 ttacggttgc cggacagcac cgcaccgacg gttaaatcgc cttttttgat tgccgtttcg
     9481 ataggatcgg gcagcggccc agagttacca atacaggtgg tacaaccgta tcccacaagg
     9541 ttaaacccca gttcgtcgag atacggtgtc agtttcgctt ttgccagata atcagaaacg
     9601 actttcgaac ccggtgccaa cgacgctttg acccacggtt gccgcttgag gccaagagtt
     9661 acggcttttt tcgccagcaa gcctgcggcc atcagcacac ttgggttaga ggtgttggtg
     9721 cacgaggtta tcgcagcaat gaccacagcg ccatcaggta actgatactg atgtccgttc
     9781 ataacataat cgactggcag gcgatcttta tgcgtggcat tcactttcag ttcgttactg
     9841 gcggcaaatg cttttggtac atcgggcagt gcaacgcgat cctgtgggcg ttttggccct
     9901 gccaggctcg cttcaacgtc attcatatcc agttctaaca tactggtaaa aatcggttca
     9961 tcgcccgggt tacgccacat gccctgcgct ttggcatatt tttcgaccaa ctcgacctga
    10021 tcttcgctgc gcccgcttaa acgcatgtaa tcgagggtta ctgcatcgat tgggaagaag
    10081 ccacaggtgg caccatattc tggcgacata ttggcaatgg tggcgcgatc cgccaacggt
    10141 agtgaatcca gaccatcacc ataaaattcg acgaatttcc ccaccacgcc atgtttgcgc
    10201 agcatttggg taacagtgag aaccaggtct gtggcggtaa taccttcacg taattttccg
    10261 gtaagtttaa agcccactac gtccgggata aacatggaa
//
LOCUS       DAZPAB010000135        10271 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZPAB010000135 DAZPAB010000000
VERSION     DAZPAB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10271
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.135"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            51..1445
                     /gene="galP"
                     /locus_tag="ACHUPG_002955"
     CDS             51..1445
                     /gene="galP"
                     /locus_tag="ACHUPG_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR0862478.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1522..2019
                     /locus_tag="ACHUPG_002956"
     CDS             1522..2019
                     /locus_tag="ACHUPG_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR0862479.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            2114..2821
                     /gene="endA"
                     /locus_tag="ACHUPG_002957"
     CDS             2114..2821
                     /gene="endA"
                     /locus_tag="ACHUPG_002957"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR0862480.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            2901..3632
                     /gene="rsmE"
                     /locus_tag="ACHUPG_002958"
     CDS             2901..3632
                     /gene="rsmE"
                     /locus_tag="ACHUPG_002958"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR0862481.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3645..4595
                     /gene="gshB"
                     /locus_tag="ACHUPG_002959"
     CDS             3645..4595
                     /gene="gshB"
                     /locus_tag="ACHUPG_002959"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR0862482.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4704..5267
                     /locus_tag="ACHUPG_002960"
     CDS             4704..5267
                     /locus_tag="ACHUPG_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR0862483.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5267..5683
                     /gene="ruvX"
                     /locus_tag="ACHUPG_002961"
     CDS             5267..5683
                     /gene="ruvX"
                     /locus_tag="ACHUPG_002961"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR0862484.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5859..6839)
                     /locus_tag="ACHUPG_002962"
     CDS             complement(5859..6839)
                     /locus_tag="ACHUPG_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR0862485.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            6857..7561
                     /gene="yggS"
                     /locus_tag="ACHUPG_002963"
     CDS             6857..7561
                     /gene="yggS"
                     /locus_tag="ACHUPG_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR0862486.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7579..8145
                     /gene="yggT"
                     /locus_tag="ACHUPG_002964"
     CDS             7579..8145
                     /gene="yggT"
                     /locus_tag="ACHUPG_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR0862487.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            8142..8432
                     /gene="yggU"
                     /locus_tag="ACHUPG_002965"
     CDS             8142..8432
                     /gene="yggU"
                     /locus_tag="ACHUPG_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR0862488.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8440..9033
                     /locus_tag="ACHUPG_002966"
     CDS             8440..9033
                     /locus_tag="ACHUPG_002966"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR0862489.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            9026..10162
                     /gene="hemW"
                     /locus_tag="ACHUPG_002967"
     CDS             9026..10162
                     /gene="hemW"
                     /locus_tag="ACHUPG_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR0862490.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2413 a         2709 c         2713 g         2436 t
ORIGIN      
        1 tactcaccta tcttaattca caataaaaaa taactatatt ggagggcatc atgcctgacg
       61 ctaaaaaaca ggggcggtca aacaaggcaa tgacgttttt cgtctgcttc cttgccgctc
      121 tggcgggatt actctttggc ctggatatcg gtgtaattgc tggcgcactg ccgtttattg
      181 cagatgaatt ccagataact tcgcacacgc aagaatgggt cgtaagctcc atgatgttcg
      241 gtgcggcagt cggtgcggtg ggcatcggct ggctctcctt taaactcggg cgcaaaaaga
      301 gcctgatgat cggcgcaatc ctgtttgttg ccggttcact gttctctgcg gctgcgccaa
      361 acgttgaagt actgattctt tcgcgcgttc tgctggggct ggcggtgggt gtggcctctt
      421 ataccgcacc actgtacctg tcagaaattg cgccggaaaa aattcgcggc agtatgatct
      481 cgatgtatca gttgatgatc actatcggga tcctcggtgc ttatctttct gataccgcct
      541 tcagctacac cggtgcatgg cgctggatgc tgggtgtgat tatcatcccg gcaattttgc
      601 tgctgattgg tgtcttcttc ctgccagaca gcccacgttg gtttgccgcc aaacgccgtt
      661 ttgttgatgc cgaacgcgtg ctgctacgcc tgcgtgacac cagcgcggaa gcgaaacgcg
      721 aactggatga aatccgtgaa agtttgcagg ttaaacagag tggctgggcg ctgtttaaag
      781 agaatagcaa cttccgccgc gcggtgttcc ttggcgtact gttacaggta atgcagcaat
      841 tcaccgggat gaacgtcatc atgtattacg cgccgaaaat cttcgaactg gcgggttata
      901 ccaacaccac cgagcaaatg tgggggacag tgattgtcgg cctgaccaac gtacttgcca
      961 cctttatcgc aatcggcctt gttgaccgct ggggacgtaa accaacgcta acgctgggct
     1021 tcctggtgat ggctgctggc atgggcgtac tcggtacaat gatgcatatc ggtatccact
     1081 ctccgtcggc gcagtatttc gccatcgcca tgctgctgat gtttattgtc ggttttgcca
     1141 tgagtgccgg tccgctgatt tgggtactgt gctccgaaat tcagccgctg aaaggccgcg
     1201 attttggcat cacctgctcc accgccacca actggattgc caacatgatc gttggcgcaa
     1261 cgttcctgac catgctcaac acgctgggta acgccaacac cttctgggtg tatgcggctc
     1321 tgaacgtact gtttatcctg ctgacattgt ggctggtacc ggaaaccaaa cacgtttcgc
     1381 tggaacatat tgaacgtaat ctgatgaaag gtcgtaaact gcgcgaaatc ggcgctcacg
     1441 attaatctcc tcaggcttcc tcccgtcgcg gaggaagccg cctcttgcag tcatcttttc
     1501 ttcgctctat cctctgccgc tatgaaaaca tcccgtctcc ctatcgccat ccaacaggcc
     1561 gttatgcgcc gcctgcggga aaaactcgcc caggccaacc tgaagctagg gcgtaactac
     1621 ccggagccaa aactctctta cacccagcgc ggaacctccg ccggaacagc ctggctggaa
     1681 agctatgaaa tccgcctcaa tcccgttttg ctgttggaaa acagtgaagc ttttattgaa
     1741 gaagtggtac cgcacgaact ggcacatttg ctggtatgga aacatttcgg ccgcgtagcg
     1801 ccacatggca aagagtggaa gtggatgatg gaaagcgtgc tgggcgttcc cgcccgtcgt
     1861 acgcatcagt tcgaactgca atccgtgcgt cgcaacacct tcccctaccg ctgtaagtgc
     1921 caggaacatc agcttaccgt acgccgccat aatcgcgtag ttcgtggcga ggccatctat
     1981 cgctgtgttc actgcggtga acagctggtt gcgaaataac cacctgaact atcaggaact
     2041 ttcctgatct ggctgattgc ataccaaaac aactttcgct acgttgctgg ctcgttttaa
     2101 cacggagtaa gtgatgtacc gttatttgtc tattgctgcg gtggtactga gcgcagcatt
     2161 ttccggcccg acgttggccg aaggtatcaa tagtttttct caggcgaaag ccgcggcggt
     2221 aaaagtccac gctgatgcgc ccggtacgtt ttattgcgga tgtaaaatta actggcaggg
     2281 caaaaaaggc gttgttgatc tgcaatcgtg cggctatcag gtgcgcaaaa atgaaaaccg
     2341 cgccagccgc gtagagtggg aacacgtcgt tcccgcctgg caattcggtc accagcgcca
     2401 gtgctggcag gacggtggac gtaaaaactg cgctaaagat ccggtctatc gcaagatgga
     2461 aagcgatatg cataacctgc agccgtcagt cggcgaggtg aatggcgatc gcggcaattt
     2521 tatgtacagc cagtggaatg gcggcgaagg ccagtacggt caatgcgcca tgaaggtcga
     2581 tttcaaagaa aaagctgccg aaccaccagc gcgtgcacgc ggtgccattg cgcgcaccta
     2641 cttctatatg cgcgaccaat acaacctgac actctctcgc cagcaaacgc agctgttcaa
     2701 cgcatggaac aagatgtatc cggttaccga ctgggagtgc gagcgcgatg aacgcatcgc
     2761 gaaggtgcag ggcaatcata acccgtatgt gcaacgcgct tgccaggcgc gaaagagcta
     2821 acctacacta gcgggattct ttttgttaac ccctacccca cgcgtacaac cgcgtgggga
     2881 gacgacgcgg atttttaact atgcgtatcc cccgcattta tcatcctgaa ccactgacca
     2941 gccattctca catcgcgctt tgcgaagatg ccgccaacca tatcgggcgc gtactgcgca
     3001 tggggccggg gcaggcgttg caattgtttg acggtagcaa ccaggtcttt gacgccgaaa
     3061 ttaccagcgc cagcaaaaaa agcgtggaag tgaaggtgct ggaaggccag atcgacgatc
     3121 gcgaatctcc gctgcatatt cacctcggtc aggtgatgtc gcgtggtgaa aaaatggaat
     3181 ttactatcca gaaatcgatc gaactcggtg taagcctcat tacgccactt ttttctgagc
     3241 gctgcggcgt taaactggat agtgaacgtc tgaacaagaa gcttcagcag tggcagaaga
     3301 ttgcaattgc tgcctgtgag cagtgtggtc gtaaccgggt gccggaaatc cgtccagcga
     3361 tggatctgga agcctggtgt gcagagcagg atgaaggact gaaactgaat cttcacccgc
     3421 gcgccagtaa cagcatcaat acgttgcctt taccggttga acgcgtccgc ctgctgattg
     3481 gcccggaagg cggtttatcg gcagatgaaa ttgccatgac tgcccgctat caatttactg
     3541 atatcctgtt gggacctcgc gttttgcgta cagagacaac tgcgctcacc gccattaccg
     3601 cgctacaagt acgatttggc gatttgggct aacggagaag aataatgatc aagctcggca
     3661 tcgtgatgga ccccatcgca aacatcaaca tcaagaaaga ttccagtttc gctatgttgc
     3721 tggaagcaca gcgtcgtggt tacgaacttc actatatgga gatgggcgat ctgtatctga
     3781 tcaatggcga agcccgcgcc catacccgca cgctgaacgt gaagcagaac tacgaagagt
     3841 ggttttcgtt cgtcggtgaa caggatctgc cgctggccga tctcgatgtg atcctgatgc
     3901 gtaaagaccc gccgtttgat accgagttta tctacgcgac ctatattctg gaacgtgccg
     3961 aagagaaagg gacgctgatc gttaacaagc cgcagagcct gcgcgactgt aacgagaaac
     4021 tgtttaccgc ctggttctct gacttaacgc cagaaacgct ggttacgcgc aataaagcgc
     4081 agctaaaagc gttctgggag aaacacagcg acatcattct taagccgctg gacggtatgg
     4141 gcggcgcgtc gattttccgc gtgaaagaag gcgatccaaa cctcggcgtg attgccgaaa
     4201 ccctgactga gcatggcact tgctactgca tggcgcaaaa ttacctgcca gccattaaag
     4261 atggcgacaa acgcgtgctg gtggtggatg gcgagccggt accgtactgc ctggcgcgta
     4321 ttccgcaggg gggcgaaacc cgtggcaatc tggctgccgg tggtcgcggt gaacctcgtc
     4381 cgctgacgga aagtgactgg aaaatcgccc gtcagatcgg gccgacgctg aaagaaaaag
     4441 ggctgatttt tgttggtctg gatatcatcg gcgaccgtct gactgaaatt aacgtcacca
     4501 gcccaacctg tattcgtgag attgaagcag agtttccggt gtcgatcacc ggaatgttaa
     4561 tggatgccat cgaagcacgt ttacagcagc agtaacccac cttagcgaga aggatctcgt
     4621 tgagactctg agtgacagcg cccttctttc cacgcatact gggcgctgtt gcttttttga
     4681 accaggaaac agaacctctg acaatgaatt tacagcatca ctttcttatt gccatgcctg
     4741 ctctccagga tccgattttc cgtcgttccg tggtctacat ttgcgaacat aataccaatg
     4801 gtgcaatggg gatcatcgtc aacaagccgc tggaaaatct caaaattgaa gggattctgg
     4861 aaaagctgaa gatcacgccg gagccgcgtg atgaatcaat ccgtctggat aaaccggtta
     4921 tgctcggcgg cccactggct gaagatcgcg ggtttatttt gcatactccg ccctccaatt
     4981 ttgcttccag cattcgcatt tcagacaaca cggtgatgac cacttcccgc gatgtgctgg
     5041 aaacgctcgg caccgataaa caaccgtctg acgtattggt ggctctgggc tatgcctcct
     5101 gggaaaaagg tcaactggaa caagaaattc tcgataacgc gtggctaacg gccccggcgg
     5161 atctgaatat tctgttcaaa acgccgattg ccgaccgctg gcgcgaggcg gcaaaactga
     5221 ttggtgtgga tattctcacc atgcctggtg tggcaggaca cgcctgatga gtggaacctt
     5281 actcgccttc gacttcggca ccaaaagcat tggcgtagcg gtcggccaac gcattaccgg
     5341 caccgctcgc cctttgcctg caattaaagc gcaggacggt acgccggact ggaacctcat
     5401 cgaacgttta ctgaaagagt ggcagcctga cgaaatcatc gtcggtttgc cgctgaatat
     5461 ggacggcacc gaacagccgt taaccgccag agcgcgcaaa tttgccaacc gtattcatgg
     5521 tcgtttcggt gttgaagtaa agctccatga cgagcgtctt agcactgtgg aagcccgttc
     5581 cggtcttttt gaacagggcg gctatcgggc gctcaacaaa ggcaaagttg actctgcctc
     5641 tgcggttatt attctcgaaa gctatttcga gcagggatat taaggcgatc taaaagcctg
     5701 gcggatagtc gttaccgcca tcttgccgga tgcggcgtga acgccttatc cagcacacat
     5761 ctggcagcgg ctgtaggtct gataagacgc gttagcgtcg catcagacat ttattgcctt
     5821 tataggccct ataaacttgc gcatcgggca tggcaacgtc acaaacgccc ttcccccacc
     5881 cgctgctgat aactctgctg aaacgttaac atccccacct tctgcccggt ttgaataaca
     5941 tgcggtaact ggtgggtttt cccttcgcga atcaaattcc ccaccgcggg agtgttaatc
     6001 agtaattcaa acagcgccac gcgtccttcc tgtttatcca cttccagctt ttgtgacagc
     6061 actgcccgta aactacccgc aagttgatta cgtacgggat ctttttcctg cgccggaaat
     6121 gaatccacca gtcgctcaac tgcctgcgcg gcaccacgtg tatgtaaggt tgccagcacc
     6181 agatgcccgg tttctgccgc cgtcagcgcc agacggattg tctcactgtc acgtagctct
     6241 ccgagcaaaa tcacatcagg atcttcccgc aatgcagccc gcaaccccga tgcgaacgtc
     6301 atacagtgca aaccaatttc ccgctgctgg atcaaacatc gctggctggc atagagatat
     6361 tccacaggat cttccagcgt cagaatatgc gcatcggcat gttgattaag atagccaacc
     6421 atcgccgcca gcgtggtaga tttgccactc cccgtagccc ccgtcaccag aatcaggcca
     6481 ttctcgctct tgagtaattc cggcaatacc ggtggtgcac caagctgttc gagctgtgga
     6541 cagtgcgaag gtaacaaccg taatgccaga gaaatacctt gccgttgcgc gaacgcactg
     6601 ccacgcaacc gctggttttc cgccagcgac acggcaaaat ccagctggcc attttccagc
     6661 aatattgccc gctgatcgtc atccagccac tcccgcagta gctcttcgac gtccggcgca
     6721 tcaaacggcg cagcttccat tctgccacga atgcgccatc gtgcgggcca ggcgctgcac
     6781 aggtgtagat ccgagacgtt atgctttaca ctaagggcca caatttcttc catattcata
     6841 ctaagatcct cggaaaatga acgatattgc gcataacctg gcacaggtcc gggacaaaat
     6901 ctcagcggct gcaacgcgtt gcggccgttc tccagaagaa attacgctgc ttgcagtcag
     6961 taaaacaaaa cctgcgagcg ccatcgcaga agccattgat gccgggcagc gtcaatttgg
     7021 tgaaaactac gttcaggaag gggtagataa aattcgccac tttcaggaac tgggcgtaac
     7081 aggattagaa tggcatttta ttggcccgtt gcagtctaat aaaagccgcc tagtggcaga
     7141 gcatttcgac tggtgtcata ccatcgaccg tttgcgcatt gctacccgcc tcaacgagca
     7201 gcgcccggca gaacttcccc ctcttaacgt actgattcaa attaatatta gtgatgaaaa
     7261 cagtaagtcc gggattcaac tggcagaact ggacgagctg gcagcggcgg tcgctgaact
     7321 accgcgttta cgtctgcgcg ggctgatggc aatccctgcg cttgagtcag aatatgtaag
     7381 gcagtttgaa gttgcacgcc aaatggctgt agcatttgcc ggactgaaaa cgcgctaccc
     7441 gcatatcgac acgctctctc tgggaatgtc ggacgatatg gaagccgcca ttgcggcagg
     7501 tagcacgatg gttcgtatcg gcactgcaat ttttggtgcg cgtgattact ctaaaaaata
     7561 aggaattaaa ggaacgccat gaatacgttg actttcctgc tttcaacggt cattgagctg
     7621 tatacaatgg tgctgttatt acgcatctgg atgcagtgtg ctcattgtga tttttacacc
     7681 cccttctcac agtttgtagt gaaggtagcg cagccaatta tcgggccact gcgccgcgtt
     7741 attccggcaa tgggtcccat tgacagcgcc tcgctgctgg ttgcctatat tctcagtttt
     7801 atcaaagcca ttgtgctgtt tgaagtggtg accttcctgc caatcatctg gattgccggt
     7861 ttactgattc tgctgaaaac catcggcttg ctgattttct gggtcctgct ggtgatggcg
     7921 attatgagct gggtaagcca gggtcgtagc ccgattgaat acgtgctgat tcagctggcc
     7981 gatccgctgc tgcgcccgat tcgccgcttg ctaccggcaa tgggtgggat tgatttctcg
     8041 ccgatgatcc tcgttctgct gctgtatgcc atcaatatgg gtgtcgcaga agtattacag
     8101 gcaaccggaa acatgctgct gccggggctg tggatggcgt tatgagtgcc gtaacagtta
     8161 atgatgacgg tctggtttta cggctctata ttcagccgaa agccagccgt gattctattg
     8221 tcggtttaca tggcgacgaa gttaaagtcg ccattaccgc gccgccggtt gacggccagg
     8281 ccaacagtca tctggtgaag tttctcggta agcaattccg ggttgccaaa agccaggtgg
     8341 tgattgaaaa aggcgaactt ggccgccaca aacaaattaa aatcattaat ccgcaacaaa
     8401 tcccgccaga aatcgcggcg ttaattaatt aggtatccta tgcaaaaagt tgtcctcgca
     8461 accggcaatg ccggtaaagt gcgtgagctg gcgtcgctgc ttagcgactt tggtcttgat
     8521 atcgtggccc aaacagacct cggcgttgat tccgctgaag aaaccggcct aacctttatc
     8581 gaaaacgcga ttctgaaagc gcgccatgcg gcaaaagtga ccggtttacc ggcaattgcc
     8641 gacgactctg gtctggcggt agatgcgctg ggtggcgcgc cgggtattta ctccgcgcgt
     8701 tattccggtg aagacgcgac cgatcaaaag aatctgcaaa aactgttgga aacactgaaa
     8761 gacgtaccgg acgaccaacg tcaggcgcgt ttccactgcg tgctggtata tctgcgtcac
     8821 gcggaagatc cgactccgct ggtatgccac ggcagctggc cgggcgtgat tactcgcgaa
     8881 ccagcgggca ctggtggctt tggttatgat ccaatcttct tcgtaccttc cgaagggaaa
     8941 accgccgccg aactgacccg cgaagaaaag agcgccattt cccaccgtgg tcaggcgttg
     9001 aaactgctgc tggacgcttt acgtaatggt taaattaccg ccgctgagtc tctacattca
     9061 catcccgtgg tgcgtgcaga aatgcccgta ctgcgatttc aactctcacg cgttgaaagg
     9121 agaagtgccg cacgacgatt atgttcagca tctgctttgc gatctggaca atgatgtggc
     9181 ttacgctcag ggccgtgaag taaagacaat ctttattggc ggtggtacgc cgagcctgct
     9241 ttccggcccg gcgatgcaaa cgctgctaga cggcgtgcgt gcgcgtttgc cgctggcagc
     9301 ggatgcagaa attactatgg aagcgaaccc aggcacggta gaagcggatc gctttgtcga
     9361 ttatcagcgt gctggtgtaa accgcatctc tattggtgtg cagagtttta gcgaagaaaa
     9421 gctgaaacga cttgggcgta ttcatggccc gcaagaagcg aaacgagcgg caaagctggc
     9481 gagcggttta gggttacgta gctttaacct tgatttgatg catggactac cggatcaatc
     9541 actggaagag gcgcttggcg atctacgcca ggccattgaa ctgaatccgc cgcatctttc
     9601 ctggtatcaa ctgactatcg aacctaatac gctgtttggc tcgcgaccgc cggtgctgcc
     9661 ggacgatgac gcgctgtggg atattttcga acaggggcat cagttattaa ccgcagcggg
     9721 ttatcagcaa tatgaaactt ccgcttacgc caaacccggt tatcagtgcc agcacaatct
     9781 caactactgg cgctttggtg actacatcgg tattggctgc ggcgcgcacg gcaaagtgac
     9841 cttcccggat gggcgcattc tgcgtaccac taaaacgcgt catcctcgtg gttttatgca
     9901 aggaaggtat ctggaaagcc agcgtgatgt cgaagccgca gataagccgt ttgagttctt
     9961 tatgaatcgc ttccgtctgc tggaggccgc gccgcgcgtg gagtttattg cgtataccgg
    10021 gctttgcgaa gatgtgattc gcccacagtt agacgaggcg attgcccagg gttatctcac
    10081 cgaatgtgcg gattactggc agataacgga acatggtaaa ttattcttaa actcacttct
    10141 tgaattattc ctcgctgagt aatcattaat gcgcctgcgg gcgcattaat cacttctttt
    10201 atccccccgt taattaacct cggcagaaat atatttctta ctgaataaat atcgacgaca
    10261 tttattatat a
//
LOCUS       DAZPAB010000136        10241 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZPAB010000136 DAZPAB010000000
VERSION     DAZPAB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.136"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..1613
                     /gene="mxiD"
                     /locus_tag="ACHUPG_002968"
     CDS             <1..1613
                     /gene="mxiD"
                     /locus_tag="ACHUPG_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HFR0862491.1"
                     /translation="EQNDIAKYVAQSDTVGSFFERFSALLNYPIVVSKQAAKKRISGE
                     FDLSNPEEMLEKLTLLVGLIWYKDGNALYIYDSGELISKVILLENISLNYLIQYLKDA
                     NLYDHRYPIRGNISDKTFYISGPPALVELVANTATLLDKQVSSIGTDKVNFGVIKLKN
                     TFVSDRTYNMRGEDIVIPGVATVVERLLNNGKALSNRQAQNDPMPPFNITQKVSEDSN
                     DFSFSSVTNSSILEDVSLIAYPETNSILVKGNDQQIQIIRDIITQLDIAKRHIELSLW
                     IIDIDKSELNNLGVNWQGTASFGDSFGASFNMSSSASISTLDGNKFIASVMALNQKKK
                     ANVVSRPVILTQENIPAIFDNNRTFYVSLVGERNSSLEHVTYGTLINVIPRFSSRGQI
                     EMSLTIEDGTGNSQSNYNYNNENTSVLPEVGRTKISTIARVPQGKSLLIGGYTHETNS
                     NEIISIPFLSSIPVIGNVFKYKTSNISNIVRVFLIQPREIKESSYYNTAEYKSLISER
                     EIQKTTQIIPSETTLLEDEKSLVSYLNY"
     gene            1632..2699
                     /gene="mxiC"
                     /locus_tag="ACHUPG_002969"
     CDS             1632..2699
                     /gene="mxiC"
                     /locus_tag="ACHUPG_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFR0862492.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            2712..4772
                     /gene="mxiA"
                     /locus_tag="ACHUPG_002970"
                     /gene_synonym="virH"
     CDS             2712..4772
                     /gene="mxiA"
                     /locus_tag="ACHUPG_002970"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HFR0862493.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            4785..5186
                     /gene="spa15"
                     /locus_tag="ACHUPG_002971"
                     /gene_synonym="spaK"
     CDS             4785..5186
                     /gene="spa15"
                     /locus_tag="ACHUPG_002971"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HFR0862494.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            5190..6482
                     /gene="spaL"
                     /locus_tag="ACHUPG_002972"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             5190..6482
                     /gene="spaL"
                     /locus_tag="ACHUPG_002972"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HFR0862495.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            6563..6913
                     /gene="spa13"
                     /locus_tag="ACHUPG_002973"
                     /gene_synonym="spaM"
     CDS             6563..6913
                     /gene="spa13"
                     /locus_tag="ACHUPG_002973"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HFR0862496.1"
                     /translation="MYRDVEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVK
                     KHIIQYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLI
                     LDELSQEDMKYGIR"
     gene            6900..7778
                     /gene="spa32"
                     /locus_tag="ACHUPG_002974"
     CDS             6900..7778
                     /gene="spa32"
                     /locus_tag="ACHUPG_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HFR0862497.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            7772..8653
                     /gene="spa33"
                     /locus_tag="ACHUPG_002975"
                     /gene_synonym="spaO"
     CDS             7772..8653
                     /gene="spa33"
                     /locus_tag="ACHUPG_002975"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HFR0862498.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            8658..9308
                     /gene="spa24"
                     /locus_tag="ACHUPG_002976"
                     /gene_synonym="spaP"
     CDS             8658..9308
                     /gene="spa24"
                     /locus_tag="ACHUPG_002976"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HFR0862499.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            9323..9583
                     /gene="spaQ"
                     /locus_tag="ACHUPG_002977"
     CDS             9323..9583
                     /gene="spaQ"
                     /locus_tag="ACHUPG_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HFR0862500.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            9583..>10241
                     /gene="spa29"
                     /locus_tag="ACHUPG_002978"
                     /gene_synonym="spaR"
     CDS             9583..>10241
                     /gene="spa29"
                     /locus_tag="ACHUPG_002978"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HFR0862501.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     I"
BASE COUNT         3266 a         1455 c         2041 g         3479 t
ORIGIN      
        1 tagaacaaaa tgacatagcg aaatatgttg ctcaaagtga tacggtagga tctttttttg
       61 aacgtttttc tgcgttatta aattatccta ttgttgttag caaacaagct gcaaaaaaga
      121 gaatatctgg ggagtttgat ttatctaatc cagaagaaat gcttgaaaaa ctgacattac
      181 ttgtgggact catatggtat aaagatggaa atgcattata catatatgac tctggagaat
      241 taataagcaa agttattttg ctggagaata tatcacttaa ttatcttatt cagtatctga
      301 aggatgctaa cctttatgat cacagatatc ctataagagg aaacattagt gataaaacat
      361 tctatatatc tggtcctccg gcattggttg aactggtagc taatacagca actttgcttg
      421 ataagcaggt ttcttctatt gggacagata aagttaattt tggggttata aaattaaaaa
      481 atacatttgt tagtgacaga acttataata tgagagggga ggatattgtc atcccagggg
      541 tggctactgt tgtagaacgt ttattaaata atggtaaggc tctgagtaac agacaagccc
      601 aaaatgatcc gatgccaccc tttaatatta cacagaaagt atctgaggat agtaatgact
      661 tttcatttag ttcagttacc aatagttcta tattagagga tgtatcactt attgcatatc
      721 cagaaaccaa ttctatatta gtcaaaggta atgatcagca aatacagatt attcgcgata
      781 ttatcactca gttagatata gccaaacgac atatagaatt atcattatgg ataatcgata
      841 ttgataaatc tgaattaaat aaccttggtg ttaattggca aggaacggct tcatttggtg
      901 attcgtttgg tgcgtcattt aatatgagct catcagcatc gataagcact ttggacggca
      961 ataagtttat tgcttcagtt atggcattaa atcagaaaaa gaaagcaaat gtagtttcaa
     1021 ggccggtgat tttgacacaa gagaatattc cggctatatt tgataataac aggacttttt
     1081 atgtgtcatt agttggtgag cgcaattctt cgttggagca tgttacgtat ggaacattga
     1141 taaatgttat acctcgcttt tcatctcggg gacaaattga gatgtcttta acgatagagg
     1201 atggaacagg aaatagtcag tcaaattaca attataataa tgaaaataca tctgtccttc
     1261 cagaagttgg aaggaccaaa atcagtacaa tagcgagagt tccacaaggc aaaagtttgt
     1321 tgataggtgg ttatacgcat gaaacgaact ctaacgaaat aatatctatt ccttttctct
     1381 cctctattcc tgtaatcggt aatgtgttca agtataaaac aagtaatatc agtaatattg
     1441 taagggtttt tctgattcaa ccccgtgaga taaaggagtc aagttattat aatactgcag
     1501 aatataaaag cctcatatct gaaagagaaa tacaaaagac tacacaaata atcccatctg
     1561 aaactacttt gctggaagac gaaaaatcat tggtttcata cttaaattac taactataaa
     1621 gtaggtgatg tatgcttgat gttaaaaata caggagtttt tagctctgca ttcattgata
     1681 agttgaatgc aatgacaaat tcagatgatg gagatgagac tgctgatgca gagcttgatt
     1741 ctggcttggc taatagcaag tatattgact catctgatga gatggcttcc gctctttcgt
     1801 catttataaa cagaagagac cttgagaaac tgaaaggaac aaatagtgat agtcaggaac
     1861 gtattttaga tggggaagaa gatgaaatta atcacaagat ttttgattta aagagaacgt
     1921 taaaagataa ccttcctcta gatcgggatt ttatagacag actaaagaga tattttaaag
     1981 atccaagtga tcaagtctta gcattaaggg aacttttgaa tgaaaaagat cttactgctg
     2041 aacaagtcga attattaact aaaattatta atgagataat atcaggtagt gaaaaaagtg
     2101 ttaatgctgg aataaattca gctatacagg ctaaattatt tggcaacaaa atgaaacttg
     2161 aaccacagct tttgcgtgca tgttatcgtg gttttatcat ggggaacata tcaacaacag
     2221 atcagtatat agaatggctt ggtaattttg gttttaatca cagacataca attgtgaatt
     2281 ttgtagagca gtcactgatt gtagacatgg attctgagaa accgagctgt aatgcttatg
     2341 agtttggttt tgtgttatct aaattaattg caattaagat gattagaact tcagacgtaa
     2401 tttttatgaa gaaactggaa tcctcaagct tgctaaaaga tggcagttta agtgcagagc
     2461 agctattgct aactttatta tatatttttc aatatccaag tgaaagtgag caaattctta
     2521 cttctgttat agaagtatca cgagccagtc atgaggattc tgtagtgtat caaacatatc
     2581 tatcttctgt taatgaaagt cctcatgata tatttaaaag tgaaagtgaa agagaaattg
     2641 cgatcaatat tctacgagag cttgtcacaa gtgcatacaa gaaagagctt tctagataac
     2701 aggagataaa agtgatccag tcttttctta agcaagtaag tactaagcct gaattaatta
     2761 tattagtgct tatggttatg atcattgcta tgttgatcat accattacct acataccttg
     2821 ttgatttttt aatagggctt aatattgtac ttgctattct cgtttttatg ggatcatttt
     2881 atattgaaag aatattgagt ttttctacat tcccttcggt tttgttgata acgacattat
     2941 ttcgtctcgc tttatcaata agtactagtc gactaatttt agttgatgcg gatgcgggta
     3001 aaattattac tacatttggc cagtttgtca ttggtgatag tctggctgta ggttttgtta
     3061 ttttttcaat agtaactgtt gtgcagttca ttgttataac aaaaggatct gaaagggttg
     3121 cggaggtagc tgctcgcttt tctcttgacg gtatgccggg gaaacaaatg agcattgatg
     3181 cggatttaaa ggctggaatt attgatgccg caggagctaa agaaagacgc agtattctgg
     3241 aacgtgagag tcaattgtat ggttcatttg atggagcaat gaagttcatt aaaggtgatg
     3301 caattgctgg catcattatt atttttgtca atttaatagg aggtatttct gttggtatga
     3361 gtcaacatgg aatgtcccta tctggtgcat tatctactta taccatacta actattggtg
     3421 acggattggt ctctcagatt cctgctctgt taatttctat aagtgcagga tttatcgtta
     3481 caagagttaa tggtgatagc gataatatgg gacgtaacat aatgtcccag atatttggga
     3541 atccttttgt tctcattgtt acatcagctc ttgccttggc gatagggatg ttgccaggtt
     3601 ttccgttttt tgtttttttc ctgatagcag ttactttgac ggctttattc tattataaaa
     3661 aggtcgtaga aaaagaaaaa agtctgtctg agtctgattc tagtggctat actggtacat
     3721 ttgatattga taatagccat gactcatctt tggcaatgat agaaaatctg gatgctatta
     3781 gttcagaaac cgttccttta attttattat ttgccgaaaa taagataaat gcaaatgata
     3841 tggaaggtct tattgaaagg ataagaagtc agttctttat tgattatggt gtcaggcttc
     3901 caactatttt atatagaaca agcaatgagc ttaaggtcga tgatattgtt ttgttaataa
     3961 atgaggtgcg tgccgatagt tttaatatat attttgacaa agtgtgtatt acagatgaaa
     4021 atggagatat agatgctcta ggtattcctg ttgtttcaac ttcatataat gaacgtgtta
     4081 tttcttgggt agacgtttca tatacagaaa atctaactaa tattgatgct aaaattaaaa
     4141 gtgctcaaga tgaattttat caccagttgt cacaagcttt attaaacaac ataaatgaga
     4201 tttttggtat acaagaaaca aaaaatatgt tagatcagtt tgaaaatcgg tatcctgatc
     4261 tattaaagga agtcttccga catgtgacta tacagagaat ttctgaggta ttacaaagat
     4321 tgcttggaga aaatatttct gttcgcaatt taaaacttat tatggagtct ttggcgcttt
     4381 gggctcccag agaaaaagat gtcataacat tagttgaaca tgtccgtgca tcactttcta
     4441 ggtatatttg tagtaaaata gctgtttctg gtgagattaa agttgtgatg ctttccggat
     4501 atattgagga tgcaataaga aaagggataa ggcaaacctc tggtggctct ttcttgaata
     4561 tggatataga ggtttcggat gaggtaatgg aaactttagc acatgctttg agagaattga
     4621 gaaatgcaaa aaaaaatttc gttcttttgg tatcagtaga tatacgtagg tttgttaaaa
     4681 gacttataga taacagattt aagagtatac tcgttatatc gtatgctgag attgatgaag
     4741 catataccat taatgtatta aagactattt agtgaggttt aaatatgagt aacattaatt
     4801 tagttcaatt agttagagat agtcttttca cgattggttg tcctccatca attattacag
     4861 atttagacag ccattcagcg attaccattt ctttggatag tatgcctgct attaatatag
     4921 ctcttgtcaa tgagcaggtt atgttatggg ctaattttga tgcaccaagt gatgtaaaac
     4981 ttcagtcatc tgcttataac atattaaatt taatgttaat gaactttagt tatagcatta
     5041 atgagctggt agagcttcat cgttctgatg agtacttaca gttaagagtt gttattaaag
     5101 atgactacgt tcacgatggg attgtttttg cagagattct gcatgagttt tatcagagga
     5161 tggaaatctt aaatggggtc ttataatcaa tgagctatac aaaattgctc actcaattat
     5221 cttttcctaa tagaatctcg gggccaatct tggaaacaag tcttagcgat gtttcgattg
     5281 gtgagatttg taacattcag gctggaattg aaagtaatga aattgttgca agagctcagg
     5341 ttgtaggatt tcatgatgaa aaaacaatat taagcttgat tggaaattct cgtggacttt
     5401 cacggcaaac gttgattaag cccactgccc agtttcttca tacgcaagtc ggccgtggat
     5461 tattgggagc agtagtcaat cctttagggg aggttactga taagtttgct gttacagata
     5521 acagtgaaat tctttatcga cctgtagata atgctcctcc gctatatagt gaaagggctg
     5581 caattgagaa gcctttttta acaggtatta aggttattga ttctttactc acgtgtggtg
     5641 aaggacagcg aatggggatt tttgcgtcag ctggttgtgg caaaactttt ctcatgaata
     5701 tgctcattga acatagtggt gctgatatat atgttattgg gttaattggt gagcgaggtc
     5761 gagaggttac tgaaacggtt gattatttga aaaactctga gaaaaaaagc aggtgtgttt
     5821 tagtatatgc aacttcggat tactcttcgg ttgatcgttg taatgctgca tatatagcca
     5881 ctgctatagc cgaatttttt aggactgaag gacataaagt agcgcttttt attgattcat
     5941 taacaaggta tgccagagca ttacgtgatg tggccttagc cgctggagaa tcacctgcca
     6001 gaagaggcta tccggtttcg gtttttgata gcttacccag acttcttgaa aggccaggaa
     6061 agttaaaggc aggtggctct attactgcat tttacactgt tcttttggag gatgatgatt
     6121 ttgctgatcc attagctgaa gaggtaagat ccattttaga tggacatata tatttgagca
     6181 gaaatctagc ccaaaaagga caatttcctg caattgattc cttaaaaagt ataagcaggg
     6241 tatttacaca ggttgttgat gaaaaacatc gtattatggc cgctgcattt cgggagctac
     6301 tttctgaaat agaagagcta aggacaatta ttgactttgg tgaatacaaa ccgggggaga
     6361 atgcctctca ggataaaatc tacaacaaaa tatctgttgt tgagagtttt ctgaagcaag
     6421 attatcgact cggttttact tatgagcaga caatggagct tattggtgaa acaattagat
     6481 aaggttttaa aaatcaaaga taaatatcaa cggtctgtta aattaattga agcacacata
     6541 ttaacactaa aaaaaaaact ctttgtatcg agatgtggag gcattagata aaaggattat
     6601 atacttcctg caactggaga atgacttaga acctgtaggc gctcagagtg ttagtcagct
     6661 tttcaataca agacgtaaga tcgctatagt aaaaaagcat attatccaat atcagtctga
     6721 acgaattctt ctaaagggaa gaattgaaga aattcaaaaa gatattgatg aggcaaatgc
     6781 ttctaaaaga aaactgcttc ataaagagag taaaatatgt aaacggattg gcttgattaa
     6841 aagaaataac tttgccaaac agttgatact tgatgagtta tcacaagagg atatgaagta
     6901 tggcattaga taatataaac ctaaatttta gtagtgacaa acagatagaa aaatgtgaga
     6961 aactatcttc aatagataat attgatagtc tcgttttgaa gaagaagagg aaggttgaaa
     7021 ttcctgagta ctctttaatt gcatctaact attttactat tgataagcac tttgaacata
     7081 agcatgataa aggagaaatt tatagtggca ttaaaaatgc gttcgaactt agaaacgaac
     7141 gagcgacata ttctgatatt ccagaatcaa tggccattaa agaaaatatt ttgataccag
     7201 atcaagatat caaagcaagg gaaaaaataa atatcggcga tatgaggggg atcttttcat
     7261 ataataagag tggaaatgca gacaagaact tcgaaagaag tcatacttct tctgtaaacc
     7321 ctgataatct gctagaatct gataatagaa atggtcaaat tggtttaaaa aatcatagct
     7381 tgtctattga taagaatatt gctgacatca tttctttact aaatggaagt gttgctaaat
     7441 catttgagct gcctgtaatg aataaaaata ctgcagacat aaccccatcc atgtcattgc
     7501 aagaaaaatc aatagttgaa aatgataaaa atgtttttca aaaaaatagt gaaatgactt
     7561 accactttaa acagtggggg gctggacatt ctgttagtat ttcagtggag tctggttctt
     7621 ttgttctaaa accgtcagat caatttgtag gaaataaact tgacttaatt ttgaaacaag
     7681 atgctgaggg taattacaga tttgatagca gtcaacataa taaggggaat aaaaataata
     7741 gtacaggata taatgaacag agtgaagaag aatgctaaga attaaacatt ttgacgctaa
     7801 cgaaaaacta cagattttat atgcaaagca actctgcgag cgtttttcga ttcagacatt
     7861 caaaaataaa tttacaggca gtgaaagttt agtcactctt acttccgtgt gtggggattg
     7921 ggtaattcgt attgatacat tatctttttt gaaaaaaaaa tacgaggtat tttcaggatt
     7981 ttctacacaa gaatctttac tgcatttatc aaaatgtgtc tttatagagt cgtcatctgt
     8041 attttcgatt ccagaactgt ctgataagat tactttccgg atcacgaatg aaatccagta
     8101 tgcaactact gggagtcatt tatgctgttt ttcctcttct ttaggtatta tttattttga
     8161 caagatgccg gtattacgta atcaagtttc tcttgactca ttgcatcatc ttttagagtt
     8221 ttgcttaggt tcatctaatg taaggctggc tactttaaaa agaattcgca ctggtgatat
     8281 aatcatagtt cagaaacttt ataatttatt attgtgtaat caagttatta ttggggatta
     8341 tattgtgaat gataataatg aggcaaaaat taatctgtca gaaagtaatg gtgagtcaga
     8401 acacacagaa gtttctttgg cattattcaa ttatgatgat atcaatgtaa aagtggactt
     8461 tattctttta gaaaaaaata tgacaatcaa tgaactaaaa atgtatgtag aaaacgaatt
     8521 attcaagttt cccgatgaca tagttaaaca tgtaaatatt aaagtaaatg gttctttggt
     8581 tgggcatggg gaacttgttt ctattgagga tggttatggt atcgagatta gttcttggat
     8641 ggtaaaggag taatctcatg ctgagtgaca tgtccctcat cgctacactt tcttttttta
     8701 cgctattacc atttttagtt gctgctggta cctgttatat aaagttttct attgtttttg
     8761 taatggtacg aaatgcactt ggtttgcagc aggttccatc aaatatgact ctcaatggta
     8821 ttgcattgat tatggctttg ttcgtgatga aacccattat tgaagctggt tatgaaaatt
     8881 atctaaatgg ccctcaaaaa tttgatacaa tatctgatat tgtccgtttt tctgattctg
     8941 gcttgatgga atataagcaa tacttgaaaa agcatacaga tcttgagtta gcacgttttt
     9001 tccaaaggtc tgaagaagaa aatgctgatt taaagagtgc agagaataat gattattcat
     9061 tgttttccct cttgcctgca tatgcgctaa gtgaaatcaa agatgcgttc aaaataggtt
     9121 tttatttata tttgccattt gttgttgttg atcttgttat ttcaagcatt ttacttgcac
     9181 ttggtatgat gatgatgagt ccaatcacaa tatcagtacc aataaaactt gtattatttg
     9241 ttgcgcttga tgggtggggt atcctgtcta aggccttgat tgaacaatat atcaatattc
     9301 ctgcttagat gatggaggta atgtgtctga tatagtttat atgggtaata aggctcttta
     9361 tttaatcctt atcttttcct tatggccagt aggtatagct acggttattg gattatctat
     9421 tggtttgtta cagacagtga ctcaactcca agagcagaca cttccttttg gtataaagct
     9481 tataggtgtc tcaatatctt tgctacttct ttctggatgg tatggtgagg ttttattgtc
     9541 tttttgtcat gaaataatgt ttttaattaa gagtggggtt tgatggacat ttcaagctgg
     9601 ttcgaaagta ttcatgtgtt tttaatactc ctgaacggcg ttttttttag attggctcca
     9661 ttatttttct ttcttccatt tttaaataac ggtataattt ctccatctat tagaatacct
     9721 gtgatttttc ttgttgcatc aggattaatt acttctggta aggtagacat aggttcttct
     9781 gtttttgaac atgtttattt ccttatgttc aaggaaataa ttgttggcct ccttctctct
     9841 ttttgcttgt ctcttccctt ttggatattt catgctgttg gtagcattat tgacaaccag
     9901 cgtggggcaa cgcttagtag ttcaattgat cctgccaatg gtgttgatac gtctgagttg
     9961 gcaaaatttt tcaatctttt ttctgcagtt gtatttctat acagtggtgg tatggtcttt
    10021 attttagaat ccatacaatt gtcttataat atatgcccgt tattttctca atgttctttc
    10081 cgtgtctcaa atatcttaac atttctgact ttattggcaa gtcaggctgt tattttagcc
    10141 agtcctgtta tgatagtatt gttactatca gaagtattac ttggtgtatt atcgagattt
    10201 gctccgcaga tgaatgcttt ttccgtatca ttaactatta a
//
LOCUS       DAZPAB010000137        10178 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZPAB010000137 DAZPAB010000000
VERSION     DAZPAB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.137"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="ACHUPG_002979"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="ACHUPG_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR0862502.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="ACHUPG_002980"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="ACHUPG_002980"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFR0862503.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="ACHUPG_002981"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="ACHUPG_002981"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR0862504.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="ACHUPG_002982"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="ACHUPG_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR0862505.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="ACHUPG_002983"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="ACHUPG_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR0862506.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="ACHUPG_002984"
     CDS             4025..4615
                     /locus_tag="ACHUPG_002984"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR0862507.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="ACHUPG_002985"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="ACHUPG_002985"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR0862508.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="ACHUPG_002986"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="ACHUPG_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR0862509.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="ACHUPG_002987"
     CDS             complement(6551..6658)
                     /locus_tag="ACHUPG_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HFR0862510.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="ACHUPG_002988"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="ACHUPG_002988"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR0862511.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="ACHUPG_002989"
     CDS             7648..7875
                     /locus_tag="ACHUPG_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR0862512.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="ACHUPG_002990"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="ACHUPG_002990"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR0862513.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..>10178)
                     /locus_tag="ACHUPG_002991"
     CDS             complement(9447..>10178)
                     /locus_tag="ACHUPG_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862514.1"
                     /translation="ISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAILV
                     LKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQSD
                     NKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRIK
                     AVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPPE
                     LFSQAYKLKNKEMNAQLDYLNRYAT"
BASE COUNT         2363 a         2627 c         2627 g         2561 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataatatt cactttttaa aaactggcat ccgctttgcg aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct catctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 ggcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa tatccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatat
//
LOCUS       DAZPAB010000138        10149 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZPAB010000138 DAZPAB010000000
VERSION     DAZPAB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10149
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.138"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..2098
                     /locus_tag="ACHUPG_002992"
     CDS             <1..2098
                     /locus_tag="ACHUPG_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR0862515.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2095..3183
                     /locus_tag="ACHUPG_002993"
     CDS             2095..3183
                     /locus_tag="ACHUPG_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR0862516.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="ACHUPG_002994"
     CDS             complement(3271..4569)
                     /gene="exuT"
                     /locus_tag="ACHUPG_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFR0862517.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5054..6466
                     /gene="uxaC"
                     /locus_tag="ACHUPG_002995"
     CDS             5054..6466
                     /gene="uxaC"
                     /locus_tag="ACHUPG_002995"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFR0862518.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6481..7968
                     /gene="uxaA"
                     /locus_tag="ACHUPG_002996"
     CDS             6481..7968
                     /gene="uxaA"
                     /locus_tag="ACHUPG_002996"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR0862519.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8051..8602
                     /locus_tag="ACHUPG_002997"
     CDS             8051..8602
                     /locus_tag="ACHUPG_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR0862520.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="ACHUPG_002998"
     CDS             complement(8607..9851)
                     /gene="sstT"
                     /locus_tag="ACHUPG_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR0862521.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         2647 a         2509 c         2654 g         2339 t
ORIGIN      
        1 tttcaacggt tatgatgcag atgatgtcag tgacagtaat actgacgtca gcgatctcta
       61 ttatcgttat gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg acaaccgcac
      121 actatttaat gcacaaggcg ggaactttac ctttaacttt ctgccactcg gtgcaatcga
      181 tgggatgcgt atcgggtcga cactgagcta tttaaaccag gcgcaaagcc agcaaggaac
      241 cccagtaatg gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta atgagcaact
      301 tttgggatcg ttttatctca atagtggttc gcaatttatt gataccagtt cctttccgcc
      361 aggcagctac agcgtggcat taaaagtcta cgaaaataac caactcaccc gcaccgagct
      421 tgtgccgttt accaaaaccg gcggtctgac tgacggaaat gcgcaatggt tcttacaggc
      481 aggtaaaact acatcacagg tttctgatga tgaaagttca gcttatcagc taggagtacg
      541 cctgccatta catccgcaat atgagctcta cgcagggctg gcgaatgctg atgatgtgag
      601 tgctttcgag ttaggtaata actggacggc agatttaggc agggtgggga atcttgcaat
      661 cagcgccagc gtgttccgta acgatgacgg cggcaaaggt gatatgcaac aggccaactg
      721 gagtaatccg ggatggccga cgttgggctt ttatcggacc aactctgacg gcgatgcttg
      781 tacaaccgac agcagagaga gctataacgc cttaagctgt tatgaaagta tttccgcgac
      841 agtttcactg aattttgtcg gctggaatat gatgctgggt tatacctgca cacaaaataa
      901 cactgatgat agtttgcgtt gggataaaca gcagagcttt gaaaataact atcttcgcca
      961 gacaacagcg caaagtatct ctgaaactgt acaacttagc gcttcccgcg ctattgtgat
     1021 gcgtgactgg attttgagta cgtcagtcgg tgttttccat cgtaatgaca acggtggcga
     1081 taacgacgac aacggcttgt acttatcgtt ttcgttatct gacacgccaa cgatggatag
     1141 caataacaac agccattcaa ccaatgtgcc tacggattat cgttatagcg aacaggatgg
     1201 cgatcaaacg tcatggcagt tatcgcatac cttttataac gattcattca gccataaaga
     1261 acttggcgta accgtcggag gcctgaacac cgataccata aacagcgcgg ttaacgggcg
     1321 ttgggatggg caatacggaa atgtctacgc taccgtatct gatagttatg accgtaagaa
     1381 tcatgatcat ctttcggcct ttacgggcac ttatagctct acgctggctg tcagtcgcta
     1441 tggcgttaat ttgggagcca gtggtacaga cgatttgctg ggtgcggtac tggtggatgt
     1501 gaaaggcttc tctgaacagg atgaagagag tcaggatctg caactcgaag cgcgggtggc
     1561 aggcagccga actttgcagc ttggtcaaag tgacagtgtg ttgttccctt atcctggatt
     1621 tcagtctggt tttgttgagg ttaacgacag tagccagggc aatcagcagg ggacaacaaa
     1681 catcattaac ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc tgcgctatcg
     1741 cgaagtgtct gccagcttta actacaacta tatcggtcgt ttattactgc cggcagcggt
     1801 gaaaaaattc ccgattgtgg ggctgaacag cgccatgtta ctggtagctg aagatggcgg
     1861 atttacactt gaaattaacg gcagcgaaaa agagctctat ctgctttccg ggcagcaatt
     1921 ccttaagtgt ccactgagtg ttgtaaagaa acgcgccagc attcgttaca gcggagatgt
     1981 tacttgtagt gtggtgactt attcacaatt accggagtcg attcaggttc aggcacagtt
     2041 gaaacagcct aaattacgtg gaaacgttca gacggcgcaa agggaggttg caccatgaga
     2101 aatcgattga ttgcggcgat attgggtttg tttggcacgc tcactggcgt tcaggcagct
     2161 cctgacgtga ccagtgaaat tacgtatgat ttggcatctg gtagagcgga ttattacttc
     2221 tggaaggatg aagcgtcggc aggaaataat ggatatatgt ggtatgaatg ttcgtatcct
     2281 gacctccaac aaacctgtac agctaatgga aatatatcga cagtacaaat ctatttaact
     2341 gaacaacgca gtgggatgcg ttggccggta aaactcaaag gatttaaaac agccattgta
     2401 agtagcgatg aagcgccgct aggatgcaag gggggcaaag ggcttcagac gaatcttaag
     2461 gattctaata gatcttcatg tacagaagat ggtcaacatt attatatata cgatacaaag
     2521 tttcttacgc tctaccttga gcagacagag atgaagaatt tgccgattgg tggcgtctgg
     2581 aaggggaaag ttaaattaca ttcgaacagc ccggcccagg actatttcgc aaatattacc
     2641 ctgaatacgc tcgaccccaa ccatattgac gtgttcttcc cggagttcgc ccacgccacg
     2701 ccaagggtgc agttagactt gcatccaaca ggaagcgtta acggcagcaa ctacgcgcaa
     2761 gatctgacta tgttggacat gtgcctgtac gatggtttta acggtaatgc catcagttat
     2821 gaaatcatgc tcaaagatga agggcgacca gctgcagggc gcagagacgg ttacttctct
     2881 atctatcgtc agggagggac caccaccgac gagggagaac gcattgatta ccgggtcaaa
     2941 atgtacaacc cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa tatggtctgg
     3001 aacagcatta acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg ctatgccgtg
     3061 atgtgtgtgc cgacaccctt gactctggca gtcgataaat tcagcgtgat ggataaacag
     3121 gccgggtact acatgggcaa attgtcagta atctttacgc cttccttgcc aaccatcaat
     3181 taaccctgca aaccggacaa acgctttttt ctatataaac gacgaagccg cctgataagg
     3241 cggcttcgtc gttataactt acgttaaacg ttaatgttgc ggtgcgggat cgttatgtgt
     3301 ttcctgcgcc acctcaatcg ccggtttgtt ctgcaatacg gtccagataa ccagcgcacc
     3361 cagcaggtca aatactgcca gaactgcgaa cagcgggctg aagccgatgg tgtcagccag
     3421 tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc cggttaaacc
     3481 gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa tcagcgcgcc
     3541 agacagtgcc tggtgggcaa aaccaccgat acacagcagc ataattgcga catacgggtt
     3601 ggtgaacaga ccgatcatac ccgggccaat catcagcaca gcacccagcg ttacgaccat
     3661 cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg gcaggtaacc
     3721 accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca tcgcgatctc
     3781 tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt taaaagtacc
     3841 ccaggccggt tctgcaagga aacgcggcag tgcgataccc cagaactgac ggttacgcag
     3901 gatctgacca acggacattt tcttcgccgt gctcacctgg tgctgggctt cctgaccatt
     3961 aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat gtttatagaa
     4021 aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa atgccatctg
     4081 ccagctgtgc atcacgattg cccataccac cagcggcggc gcaatcatcg caccaatcga
     4141 agaacctacg ttaaagtagc ctactgcgat ggaacgctct ttcgccggga accattcgga
     4201 gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc
     4261 aacagctagg ccaccccagc tacctgccag cgcggttgca ccacagaaca cggcccacag
     4321 cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat aacctgctac
     4381 cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat actgttgggt
     4441 ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag
     4501 gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta accctttaat
     4561 tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc catcatcccg
     4621 ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaaaccg gcacgccccg
     4681 aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc cgattcatct
     4741 aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga cggcagaaag
     4801 ataacttgtc atacaacctt aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg
     4861 gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagcgaga tttaccactt
     4921 tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc ttcggactct
     4981 ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct cactcgttga
     5041 gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata ccgaatttgc
     5101 ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc attgccattt
     5161 gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca tctggttgaa
     5221 gggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggctgagc gtctgtgtac
     5281 tggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc cgcacactat
     5341 cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgttcg gtatcactgg
     5401 caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg aactgctggc
     5461 gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac
     5521 caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag acggctcttt
     5581 caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaatatcg aacaggcgac
     5641 ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc gccgctttgc
     5701 tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt
     5761 gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg aactggacag
     5821 cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa
     5881 aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg tacagcagta
     5941 ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg ggccggatgt
     6001 aggctttgac tccatcaacg accgtccgat ggcggaagag ttgtctaagc tgctgagcaa
     6061 gcagaacgaa gaaaacctgc tgccgaaaac cattctctac tgcctgaacc cgcgcgataa
     6121 cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca
     6181 gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc agatgaccca
     6241 actggcgcag ctcggtctgc tgagccgctt tgtcggtatg ctgactgaca gccgtagctt
     6301 cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga tcggtcgctg
     6361 ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg tgaaaaatat
     6421 ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc tggggttgat
     6481 atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc agatttggct
     6541 gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga tgttgctcgt
     6601 ggacataaat ttgcgttaac ggatatcgca aaaggggcca acgtcatcaa atatggcctg
     6661 ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc ccacaatacg
     6721 cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ttgattttca ggatctgcct
     6781 gcgcaagcgg cagatcctga agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg
     6841 cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc
     6901 cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta ccgacggcgt gttcctcttc
     6961 agccacacct atggctgctc acaactgggc gacgatcaca tcaacacccg caccatgctg
     7021 caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa
     7081 aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat
     7141 ttcatgatct gccaacagca ggatgatgag atcgaagccg ggatcgagca tttgcatcag
     7201 ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga actgaagttt
     7261 ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc gatgctgggg
     7321 cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga agtgccggag
     7381 atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac gtttgaaaaa
     7441 ctggtcacca tggtcaacga cttcaaacag tactttattg cccatgatca gccgatctac
     7501 gaaaacccat caccggggaa caaagcgggc ggtatcacca cgctggaaga caaatcactt
     7561 ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt
     7621 ctgaaaacgc cagggctgaa cttgttaagt gcgccgggta acgatgccgt agcgaccagc
     7681 gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgcgg cacgccatac
     7741 ggtggatttg tgccgacggt gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa
     7801 cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc gcagttgctg
     7861 gaagaattta tcgacaccat cgttgagttt gccaacggta agcaaacctg caacgagcgt
     7921 aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag
     7981 ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc atacctgcta
     8041 aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg cctttctgat
     8101 tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta
     8161 tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg gtgccagtgc
     8221 catccttaat gccattcgta cattgattac cttacgcacg cgcagcttat gggtaatggc
     8281 gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact
     8341 attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta aagggctcac
     8401 catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact tctgggcggg
     8461 gtcgataggc ggcacgatga ttgagggtag ttttctgctt atgaatggcc tgaatatcat
     8521 tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga aaacccctcc
     8581 cgccatagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc cagacgatcg
     8641 tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt
     8701 tcgcaagagt cctgcaatac gccgataata aagccgacgg caaccacctg catggcgata
     8761 tcgttcgaaa taccgaacat attacaggcc agtgggatca gcagcaaaga accccccgcc
     8821 acgccggatg cgccacaggc acacagagaa gccactacgc tcaacagcag cgctgtgggc
     8881 agatcgaccg gaatacccag cgtattaacc gcagccagcg tcaacacggt aatggtgatt
     8941 gctgcgcccg ccatattgat ggtggctccc agcggaatag aaactgaata ggtatcgcga
     9001 tccagattca gcttttcaca cagcgccata ttcaccggaa tgttcgctgc agagctgcgg
     9061 gtgaagaagg catacacacc gctttcgcgc aggcacagca gcaccagcgg gaacgggtta
     9121 cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag taacatacag
     9181 ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc
     9241 agggtagaag aaaccagccc aaaaataccg aacggtgcga agcgaatgac cagtttcacc
     9301 ataaaggtaa cggcattcga catatcattg accaggtttt tggtggtctc gttaccgtga
     9361 cgcagtgcga agccgaggcc gatcgcccac accagaatcc cgatgtagtt acctttcagc
     9421 agggcgtcga tggggttgga aaccatgctc attaccagtc cgcgcatcac ttcgacaatg
     9481 cctgacggcg gcgaaatatc acccgcgcta ctggacaagt gcagggtaga agggaaggca
     9541 aagctgaaga ctactgcggc cagagctgca gagaaggtgc ccagcagata gaggaacaaa
     9601 ataggacgga tattggtttt ctgcccgtgc tggtggttag caatagatgc cattaccagc
     9661 atcaacacca ggatgggggc aacagctttc agtgcgccga cgaacaaagt acctaacaga
     9721 ccaacagctt ccgccgcggg ttttgagatc catgccagaa gaatccccag aacaaggccg
     9781 accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc cggtgaacgt
     9841 tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac atccctggtg cgttctgtcg
     9901 acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa agttacagct
     9961 tcgggaagcg aaaaatgctg gattttaaag agaggtcatg ttttgcaact taatggttgc
    10021 atttatttaa cataaacaga gggaaggact ggctactaag gctgattaga taacatatgg
    10081 gcaaaactat ttggaatatg agaacggttg cgctccttta acgtgttatg ccatataaag
    10141 tcactggcg
//
LOCUS       DAZPAB010000139        10125 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZPAB010000139 DAZPAB010000000
VERSION     DAZPAB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.139"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..686
                     /locus_tag="ACHUPG_002999"
     CDS             <1..686
                     /locus_tag="ACHUPG_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HFR0862522.1"
                     /translation="RYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSY
                     RGEVGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLS
                     ERPVMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGN
                     CLDNAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEY
                     RNQTYMPRV"
     gene            783..935
                     /gene="hokE"
                     /locus_tag="ACHUPG_003000"
     CDS             783..935
                     /gene="hokE"
                     /locus_tag="ACHUPG_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR0862523.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            1286..1438
                     /gene="hokE"
                     /locus_tag="ACHUPG_003001"
     CDS             1286..1438
                     /gene="hokE"
                     /locus_tag="ACHUPG_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR0862524.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(1560..2180)
                     /gene="entD"
                     /locus_tag="ACHUPG_003002"
     CDS             complement(1560..2180)
                     /gene="entD"
                     /locus_tag="ACHUPG_003002"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR0862525.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(2355..4595)
                     /gene="fepA"
                     /locus_tag="ACHUPG_003003"
     CDS             complement(2355..4595)
                     /gene="fepA"
                     /locus_tag="ACHUPG_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR0862526.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4838..6040
                     /gene="fes"
                     /locus_tag="ACHUPG_003004"
     CDS             4838..6040
                     /gene="fes"
                     /locus_tag="ACHUPG_003004"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR0862527.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            6043..6261
                     /gene="ybdZ"
                     /locus_tag="ACHUPG_003005"
     CDS             6043..6261
                     /gene="ybdZ"
                     /locus_tag="ACHUPG_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR0862528.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            6258..10103
                     /gene="entF"
                     /locus_tag="ACHUPG_003006"
     CDS             6258..10103
                     /gene="entF"
                     /locus_tag="ACHUPG_003006"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR0862529.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         2270 a         2617 c         2762 g         2476 t
ORIGIN      
        1 gccgatacgg ataccgtagg gtaacgctgt ctcttcatcg agaagggaaa cagattaacc
       61 ataaagctgt tcagcgcctg atgggaaccc tctcacttaa agcagcgatt aaggtcaagc
      121 gataccgctc ttacagagga gaggtagggc aaaccgcccc taatgttctc caaagagatt
      181 tcaaggctac gcggccaaac gagaagtggg ttaccgatgt tactgaattt gcagtcaatg
      241 ggcgcaagct gtatttgtct ccagtaatag atctcttcaa caacgaagtt atttcttaca
      301 gcctttcgga aagaccagtg atgaacatgg ttgagaatat gctcgatcag gcattcaaaa
      361 agcttaatcc tcacgagcat cctgttctgc actctgacca gggatggcag tatcgtatga
      421 gaagatatca aaatatcctt aaagaacatg gtattaaaca aagcatgtcc agaaaaggca
      481 attgtctgga taatgctgtg gtggagtgtt tctttggaac cttaaagtcg gagtgttttt
      541 atcttgatga gttcagtaat ataagcgaac tgaaggatgc tgttacggaa tatattgaat
      601 actataacag cagaagaatt agcctgaaat taaaaggtct gactccaatt gaatatcgga
      661 atcagaccta tatgcctcgt gtataactgt ccaacttttt ggggtcagta caccctgggg
      721 ctgccactcc aaaccagaca atttggatgg tagtcccttc ttcgcatgga ggtaatataa
      781 acatgctgac gaaatatgcc cttgtggcag tcatagtgct gtgtttgacg gtactgggat
      841 ttacgcttct ggccggagac tcgctgtgtg agtttacggt gaaggaacgt aatattgagt
      901 tcagggctgt tctcgcttac gaaccgaaga agtagccgtt gtgcggggag taatccccgc
      961 atatccggtt gtcaggtcag gatggtaagg cacctgcttt acactttcgc ccgcggtcag
     1021 tgatggctgc gggcgatttc gttttaagag caatgatgag ttttgccgac agaagtccga
     1081 aacaggactc actgtataaa taaacagcta ttttgctaag gaagggtaag ataacggcgg
     1141 gtgcctgaag ctttccggtt tcaggttaac tctgaggttt ggaaagatga agccccaaga
     1201 gatatttcta tcaatcctgg ggctgccact ccgaacctca acaactcgga tggtagtccc
     1261 ttcttcgcat ggaggcaata taaacatgct gacgaaatat gcccttgtgg cagtcatagt
     1321 gctgtgtttg acggtgctgg gatttacgct tctggtcgga gactcgctgt gtgagttcac
     1381 cgtaaaggaa cgtaatattg agtttaaggc tgttcttgct tacgaaccga agaagtagcc
     1441 gttgtgcggg gagtaatccc cgcatatccg gttgtcaggt cagagtggta aggcacctgc
     1501 tttacacttt cgcccgcagt cagtgatggc tgcgggcgaa tcgtaccaga tgttgtcaat
     1561 taatcgtgtt ggcacagagt tatgactatt ttttctttta tctgccagtg cacagcaaac
     1621 atctcattct cacgatgaat gatgacctgc tgtttattcc agctaattat ctgatagtcc
     1681 agaaaacctg catcagtttg gatctcactt gccttaaatg cgctctcttt ggcggaaaat
     1741 gcaagtgtca gcgccagaga aaaggctaaa ccgcagtctg cgagtcgctc gtgttctgct
     1801 ggtgtaataa tgttgtctgt caattcggtc gctgtttgcg cagaaaaaat ttcttcgata
     1861 tcaacgccaa tcggttgacg ggaaaccacg gccaatgccg tagccccgca gtggctaata
     1921 ctgccgtata cctccgcagg ccacaccggt tggcgtagct cgccgattgc gggcacacat
     1981 ttatagccat attcccgcaa ggcataaaca gcagcgatcc gtccggctaa atgctctgtt
     2041 ttacgtttac gtccagcgtg ttgcagttgt gcgtagtgcg gcagccagag taaatcctgc
     2101 tcacaaaaat tcgccggatc gaactcaaca aaatgcagcg tatgtccggc aaaggggagg
     2161 gaggtatgcg tagttttcat atcgaccata ttcgagactg atgacaaacg caaaactgcc
     2221 tgatgcgcta cgcttatcag gcctacgtga tcctgcaata tattgaattg gcaagatctt
     2281 tgtaggccgg ataaggcgtt cacgccgcat ccggcgtgaa cgacgcgcac tttgtcaaca
     2341 atctgacgtt agcatcagaa gtgagtatta atgctcatat accacgtacg tcccggctcg
     2401 ttataggtat acgcgccggc accggcgata tagttggctc ctgccagatc gcccgtggtc
     2461 tgggcattac ctgcacgcca caaacgtttg tcgaacaggt tgtccacgcc gccggtcaga
     2521 ctgacattct tcgtcacatc ccaggtcgcg ctcagaccca caatgctgta aggactgatt
     2581 tctttggttt ccgggccaac cgctggctga cctttatagt tgtacttctt cggctgctgc
     2641 ttgccgtacc aggtgaaggt cgtttgcatc gacaaatctt cccgtgcctg ccagctcagc
     2701 gttgagttca acgtgtactc cgggatgatc gacaaacggt cgcccgtggt tttgttttca
     2761 ctcttcagca tataagtgat gttattggtc cacatcaccg tttcgctaac tggtacgttt
     2821 aacgatcctt ccagaccttc aaccactgct ttcggcacgt tatcccactg atagagatca
     2881 gtgccgactg cgttttgccc tacagccaca tagcccgctt caatcttatt gcgataatcg
     2941 ttacggaacc aggtgacgcc cgccagccag ccgtcgcgtt tgaactccag accaatctct
     3001 ttgttgatgc tggtttctac tttcaggtca tcgttacctt gcagatagca gccgcccgcg
     3061 ctggcatagc agccctgacc tttactgtag agaatgtagt tcgggttagt ctggtatagg
     3121 ctcggcgctt tataggcgcg ggcaatgcct attttcagcg tgaagtcatc gcctaaacct
     3181 tgcgatatgt tcagcgccgg gctccagtta ttgccgacaa tactgtgatg atcgaaacgc
     3241 agccccggcg ttacgatggt gctgtcagtc agctccatgt tgttttcggc aaacagcgag
     3301 aaaatttctg cttttgaata cgggctacgg tcggtggcac tcacgccatc aatagcgcca
     3361 ccggtattcg ttccggtcag tgcttgggtg ttggaactta agtccttcat ccgttgctga
     3421 ttccactccg tacccagcgt cagcgtctgg ttaacgagga aatcaatcgg caggttaact
     3481 tcgctgtgca gcatcacgtc atcgagatcg atatcgacga aatcctgtgt cgctttttcg
     3541 ttaaatttcc cttcggtacc gcccgccaga ccttccggaa tacgcgagtt acgggtgtgt
     3601 tcatactgca cccagttgct ggtggtcacg ccgttatccc agccaccgtt ccaggtcagc
     3661 gcgtagttct ggcgatacag gcggttggtt tcatcgccat atttcgagcg agtgtaagcg
     3721 tcagagttgg tgttctgggt atcgcccgca tacaggttac cctggcggct gtaacccgct
     3781 tccagttcca gcgattgcaa tggtgcgaag tcccagcgca ccacgccgtt aatatcttta
     3841 ttgatcaccc cttcgcgccc ggctggtaac gtcgtggcat acgttccggc acgcgcggac
     3901 tgatggccct ggttgatatc ccacgcgtca gcctgagttt tgtcgaggtt gccatacaag
     3961 cggaagctga attcgtcgcc cagcggaccg gtcaggctaa agttagtgcg tttggtggca
     4021 ccttcctctt tatgttctgg cgcattgaaa tatgcgtccc aggagccgtg ccactcgccg
     4081 ctgccttttt tggtaatgat gttaaccacg ccgcccgccg cgccgttgcc ataacgcgca
     4141 gctgccggac cacgcagaac ttcaatacgt tcaatcattt caggtggcac ccaggaggta
     4201 tcaccacggg tatcgcgctc gccacgccag ccctgacgca ccgagttacg gctgcttacc
     4261 ggcttgccgt caatcaaaat cagcgtgttt tccggaccca taccgcgaat atcaatctgt
     4321 cggttattcc cacgctgacc actggtggag ttaccggtca ggttaacgcc tggcatggta
     4381 cggatgatct ccgacacatc gcgggcaacc gggtttttgc ggatttcatc tgcggtgatg
     4441 gtcgaaacgc caggagcctg caagttctgc tcggcggcgg taacgacaat ggtatcgtca
     4501 tgtgaaacag gtgtatcggt cggctcttgt gcctgcgcta ccccataaat ccccagattg
     4561 accaacaagg ccagggaatg aatcttcttg ttcattgttt tattcctgca tttttgccac
     4621 gaattgcaac tgtcgggcat ggtcgtcatc aacacgacgc atcccgttac cgcgaaaacc
     4681 tttgatcctg aaagacacgc agtgcagttg gttaattaat gtccgcgctt cccacggcgc
     4741 gccattacgc tattgcaaat gcaaatagtt attaataaca ttatcaatat atttctgcaa
     4801 tcaatgaaaa attgcacagt aaacatgggg ttatggtgtg acggcgttaa aagtaggaag
     4861 tgagagctgg tggcagtcga aacatggccc ggaatggcag cgtctgaatg acgaaatgtt
     4921 tgaggtcact ttctggtggc gtgatcccca aggttctgaa gaatactcga cgataaagcg
     4981 cgtatgggtc tacatcactg gtgtgaccga tcatcatcag aacagccagc cccagtcgat
     5041 gcagcgaatt gcaggtactg atgtctggca gtggacgaca caactcaatg ccaactggcg
     5101 cggcagctac tgctttattc ccaccgaacg cgatgacatt ttttctgcac catcccccga
     5161 tcgcctcgaa ttacgtgaag gctggcgaaa actattaccc caggcaatag ccgatccgct
     5221 gaatccacaa agctggaaag gcgggctagg gcacgctgtt tctgcactcg aaatgccgca
     5281 agcgcctctg caaccgggat gggattgtcc gcaagcgcca gaaataccag ccaaagaaat
     5341 tatctggaaa agtgaacggt tgaaaaattc acgtcgtgta tggattttta ccaccggcga
     5401 tgtaacagca gaagaacgcc cgctggcagt tttgctcgat ggcgaatttt gggcgcaaag
     5461 tatgcccgtc tggcctgtgc tgacttcgct gacccatcgt cagcaacttc ctcccgccgt
     5521 gtatgtgttg atcgacgcta tcgacaccac gcaccgcgcc cacgaactgc cgtgtaatgc
     5581 ggatttctgg ctggcagtac agcaagagtt attacccctg gtgaaagtta ttgccccttt
     5641 tagcgatcgc gccgatcgca ccgtggtcgc cgggcagagt tttggtgggc tgtccgcgct
     5701 gtatgccgga ctgcactggc ctgaacgctt tggctgtgta ttaagccagt caggatcgta
     5761 ctggtggccg catcggggcg ggcagcaaga gggcgtgtta cttgaaaagc taaaagctgg
     5821 tgaagttagc gccgaaggtc tgcgcattgt gctggaagcg ggtattcgcg agccgatgat
     5881 catgcgggcc aatcaggcgc tgtatgcgca attacaccct ataaaagaat ccattttctg
     5941 gcgtcaggtt gacggcggac atgatgcgct ttgttggcgc ggtggcttga tgcaggggtt
     6001 aatcgacctc tggcaaccac ttttccatga caggagttga atatggcatt cagtaatccc
     6061 ttcgatgatc cacagggagc gttttacatt ttgcgcaatg cgcaggggca attcagtctg
     6121 tggccgcaac aatgcgcctt accggcaggc tgggacattg tgtgtcagcc gcagtcacaa
     6181 gagtcctgcc agcagtggct tgaagcccac tggcgtactc tgacaccggc gaatttcacc
     6241 cagttgcagg aggcacaatg agccagcatt tacctttggt cgccgcacag cccggcatct
     6301 ggatggcaga aaaactgtcg gatttaccct ccgcctggag cgtggcgcat tacgttgagt
     6361 taaccggaga ggttgatgcg ccattactgg cccgcgcggt ggttgccgga ttagcgcaag
     6421 cagatacttt gcggatgcgt tttacggaag ataacggcga agtctggcaa tgggtcgatg
     6481 atgcgctgat attcgaactg ccagaaatta tcgacctgcg aaccaatatt gatccgcacg
     6541 gtactgcgca ggcattaatg ctggcggatt tgcaacaaga tttgcgcgtc gatagcggta
     6601 aaccactggt ctttcatcaa ctgatacagg tggcagataa ccgctggtac tggtatcagc
     6661 gttatcacca tttactggtc gatggcttca gttttccggc cattacccgc cagatcgcca
     6721 acatttactg cgcattgctg cgtggcgaac caacgcctgc ttcgccgttt acgcctttcg
     6781 ctgatgtagt ggaagagtac cagcaatacc gcgaaagcga agcctggcag cgtgatgcgg
     6841 cattctgggt ggaacagcgt cgccaactgc caccgcccgc gtcactttct ccggcacctt
     6901 tagcggggcg cagcgcctcg gcagatattt tgcgtctgaa aatggaattt accgacgggg
     6961 aattccgcca gctggctacg caactttcag gtgtgcagcg taccgattta gcccttgcgc
     7021 tgacggcttt gtggctgggg cgattgtgca atcgcatgga ctacgccgcc ggatttatct
     7081 ttatgcgtcg actgggctcg gcggcgctga cggctaccgg acccgtgctc aacgttttgc
     7141 cgctgggtat tcatattgcg gcgcaagaaa cgctgccgga actggcaacc cgactggcaa
     7201 cccgactggc agcacaactg aaaaaaatgc gtcgtcatca acgttacgat gccgaacaaa
     7261 ttgtccgtga cagcgggcga gcggcaggtg atgaaccgct gtttggtccg gtactcaata
     7321 ttaaggtatt tgattaccaa ctggatattc ctggtgttca ggcgcaaacc cataccctgg
     7381 caaccggtcc ggttaatgac cttgaactgg ccctgttccc ggatgaacac ggtgatttga
     7441 gtattgagat cctcgccaat aaacagcgtt acgatgagcc aacgttaatc cagcatgctg
     7501 aacgcctgaa aatgctgatt gcccagttcg ccgcggatcc ggcgctgttg tgcggcgatg
     7561 tcgatattat gctgccaggt gagtatgcgc agctggcgca gatcaacgcc actcaggttg
     7621 agattccaga aaccacgctg agcgcgctgg tggcagaaca agcggcaaaa acaccggatg
     7681 ctccggcgct ggcagatgcg cgttacctgt tcagctatcg ggaaatgcac gagcaggtgg
     7741 tggcgctggc gaatctgctg cgtgagcgcg gcgttaaacc aggggacagc gtggcggtgg
     7801 cactaccgcg ctcggtcttt ttgaccctgg cactccatgc aatagttgaa gctggcgcgg
     7861 cctggctacc gctggatacc ggctatccgg acgatcgcct gaaaatgatg ctggaagatg
     7921 cgcgtccgtc gctgttaatt accaccgacg atcaactgcc gcgctttagc gatgttccca
     7981 atttaaccaa cctttgctat aacgccccgc ttacccccca gggcagtgcg ccgctgcaac
     8041 tttcacaacc gcaccacacg gcttatatca tctttacctc tggctccacc ggcaggccga
     8101 aaggggtaat ggtcgggcag acggctatcg tcaaccgcct gttgtggatt caaaatcatt
     8161 atccgctcac aggcgaagat gtcgttgccc aaaaaacgcc gtgcagtttt gatgtctcgg
     8221 tgtgggagtt tttctggccg tttattgccg gggctaaact ggtgatggct gaaccggaag
     8281 cgcaccgcga cccgctcgct atgcagcaat tctttgccga atatggcgta acgaccacgc
     8341 actttgtgcc gtcgatgctg gcagcatttg ttgcctcgct gactccgcaa accgctcgcc
     8401 agagttgcgt gacgttgaaa caggttttct gtagtggtga ggccttaccg gctgatttat
     8461 gccgcgaatg gcaacagtta accggcgcgc cgttgcataa cctgtatggc ccgacggaag
     8521 cggcggtaga tgtcagctgg tatccggctt ttggcgagga actggcacag gtgcgcggca
     8581 gcagtgtgcc gattggttat ccggtatgga atacgggtct gcgtattctt gatgcgatga
     8641 tgcatccggt gccgccgggt gtggcgggtg atctctatct caccggtatt caactggcgc
     8701 aggggtatct tggacgaccc gatctgaccg ccagccgctt tattgccgat ccttttgctc
     8761 ctggtgaacg gatgtatcgt accggagacg ttgcccgctg gctggataac ggcgcggtgg
     8821 agtacctcgg gcgcagtgac gatcagctaa aaattcgcgg gcagcgtatc gaactgggcg
     8881 aaatcgatcg cgtgatgcag gcgctgccgg atgtcaaaca agccgttacc cacgcctgtg
     8941 tgattaacca ggcggcagcc actggtggtg atgcgcgtca gttggtgggc tatctggtat
     9001 cgcaatcggg cctgccgttg gataccagcg cattgcaggc gcagcttcgc gaaacattgc
     9061 cgccgcatat ggtgccagtc gttctgctgc aactgccgca gttgccactt agcgccaacg
     9121 gcaagctgga tcgcaaagcc ttaccgttgc ctgaactgaa ggcacaagcg ccagggcgtg
     9181 cgccgaaagc gggcagtgaa acgattatcg ccgcggcatt cgcgtcgttg ctgggttgtg
     9241 acgtgcagga tgccgatgct gatttctttg cgctgggcgg tcattcgcta ctggcaatga
     9301 aactggcagc gcagttaagt cgacagtttg ctcgtcaggt gacgccgggg caggtgatgg
     9361 tcgcgtcaac cgtcgccaaa ctggcaacga ttattgatgg tgaagaggac agctcccggc
     9421 gcatgggatt cgaaaccatt ctgccgttgc gtgaaggtaa tggcccgacg ctgttttgtt
     9481 tccatccggc gtccggtttt gcctggcagt ttagcgtgct ctcgcgttat ctcgatccac
     9541 aatggtcgat tattggcatt cagtcgccgc gccctcatgg acccatgcag acagcgacaa
     9601 acctggatga agtctgcgaa gcgcatctgg caacgctact tgagcaacaa ccgcacggca
     9661 ttgcggcgcg gctgcgtgcc cgtggcgaac aggtggcatt tcttggtttg ctggatacct
     9721 ggccgccaga gacgcaaaac tggcaggaaa aagaggctaa tggtctggac ccggaagtgc
     9781 tggcggagat taaccgcgaa cgcgaggcct tcctggcggc acagcaggga agtacttcaa
     9841 cggagttgtt taccaccatt gaaggcaact acgctgatgc tgtgcgcctg ctgacgactg
     9901 ctcatagcgt accgtttgat ggtaaagcga cgctgtttgt tgctgaacgc acgcttcagg
     9961 aaggtatgag ccccgaacgc gcctggtcgc cgtggattgc ggagctggat atctatcgtc
    10021 aggattgtgc gcatgtggat attatctctc caggggcgtt tgtaaaaatt gggccgatta
    10081 ttcgcgcaac gctaaacagg taaattaata ttatctataa accca
//
LOCUS       DAZPAB010000140         9796 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZPAB010000140 DAZPAB010000000
VERSION     DAZPAB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9796
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.140"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            383..1501
                     /locus_tag="ACHUPG_003007"
     CDS             383..1501
                     /locus_tag="ACHUPG_003007"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR0862530.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1567..1815
                     /locus_tag="ACHUPG_003008"
     CDS             1567..1815
                     /locus_tag="ACHUPG_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFR0862531.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1880..2248
                     /locus_tag="ACHUPG_003009"
     CDS             1880..2248
                     /locus_tag="ACHUPG_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR0862532.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFCPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2342..2995
                     /gene="nfsB"
                     /locus_tag="ACHUPG_003010"
     CDS             2342..2995
                     /gene="nfsB"
                     /locus_tag="ACHUPG_003010"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR0862533.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3103..4350
                     /gene="ybdG"
                     /locus_tag="ACHUPG_003011"
     CDS             3103..4350
                     /gene="ybdG"
                     /locus_tag="ACHUPG_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR0862534.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="ACHUPG_003012"
     CDS             complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="ACHUPG_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR0862535.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="ACHUPG_003013"
     CDS             complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="ACHUPG_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR0862536.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKQLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="ACHUPG_003014"
                     /pseudo
     CDS             complement(9050..9568)
                     /gene="cusB"
                     /locus_tag="ACHUPG_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2397 a         2717 c         2375 g         2307 t
ORIGIN      
        1 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
       61 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      121 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      181 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg
      241 ccacattgaa aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa
      301 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      361 aacgtcaact taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac
      421 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      481 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
      541 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
      601 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
      661 aatttgcggc ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa
      721 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
      781 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg
      841 cttgtcacgg tttgtgccgc actttattgc cctttccgcc gcgtcgccat atatgcaggg
      901 aacggatacg cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg
      961 cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtctgagtta
     1021 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
     1081 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat
     1141 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1201 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga
     1261 aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg
     1321 attgctggaa aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc
     1381 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1441 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
     1501 agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac
     1561 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     1621 ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg
     1681 gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg
     1741 tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
     1801 tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc
     1861 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc
     1921 tcttgagtta ccctttgtcg agctttgctg gcctttttgc ccggagttcg atgtttttaa
     1981 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2041 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg
     2101 gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc
     2161 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2221 cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta
     2281 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt
     2341 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
     2401 aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag
     2461 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     2521 tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca
     2581 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     2641 ccaggaagat gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg
     2701 tcgcaagttc ttcgcctata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     2761 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     2821 cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga
     2881 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     2941 cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc
     3001 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
     3061 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3121 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
     3181 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3241 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3301 aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta
     3361 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
     3421 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3481 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     3541 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     3601 attctaatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     3661 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     3721 tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     3781 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     3841 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     3901 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     3961 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4021 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4081 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc
     4141 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     4201 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4261 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4321 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4381 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4441 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4501 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     4561 acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa
     4621 aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg
     4681 ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     4741 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     4801 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     4861 ccccgctgtt atacaccgac agcgatgcca ctagaatgac gaagttcagc gcagaagcta
     4921 ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     4981 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc
     5041 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5101 ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5161 tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5221 ctttctcgcc gccgtaacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5281 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5341 ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5401 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5461 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     5521 aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc
     5581 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     5641 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg
     5701 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     5761 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     5821 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     5881 cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     5941 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggtgcg
     6001 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6061 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac
     6121 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc
     6181 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca
     6241 agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac
     6301 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6361 cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt
     6421 ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6481 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6541 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6601 agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc
     6661 ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc
     6721 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     6781 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     6841 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcagcaagc
     6901 gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg
     6961 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7021 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc
     7081 acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc
     7141 tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7201 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7261 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7321 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7381 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7441 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg
     7501 ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat cactacgatc
     7561 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7621 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7681 ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7741 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagctg tttatgcgca
     7801 ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc
     7861 agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac
     7921 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacggcgcg
     7981 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc
     8041 cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta
     8101 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8161 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc
     8221 cggcgcatct tcgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca
     8281 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgtag atagccgctg
     8341 gcgcgcacca tatattctgc ttccgccagt tcgattgacg aaccgcccgc ttcctggttt
     8401 gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctga
     8461 ggatcgataa ccacctgata ctctttcacc acaccgccta ccgacgccac ttccgcaacg
     8521 tcaggaatgg ttttcagctc atatttgaga aaccagtcct gcaatgagcg taaatcggca
     8581 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
     8641 gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg
     8701 tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac
     8761 acataagagt cgccaaactg cgagaaacca cgcacagtct tcgcgccagg caccgacaac
     8821 atggtggtgg ttagcggata agtcacctga ttttcaacga tttgcggtgc ctggccggga
     8881 tagctggttt taataatcac ctgcacatcg gagagatccg gcaacgcatc cactggcgta
     8941 ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa
     9001 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
     9061 gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
     9121 tcaggaacag gccgccggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
     9181 tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat
     9241 caacggtaat cacccgctgt tcgttgccgg tatcaatcag cgcctgtgac ggaatgagca
     9301 gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct
     9361 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
     9421 gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga acggtgaggg
     9481 taaactgcga ggcatctttc accagccagg cgatggactc cgggatcgca gcagtgaccc
     9541 acaccgggtc cataccctga attcgcgatt acgcgcaatc cccctgagaa ccgccatgaa
     9601 caagggtttc atgatttgtg attttagttt ggtacgcaat ttggtacaca acactatttt
     9661 cacttcagcg ggtagtcatc aaactcacca taacgagcat cgttgatgat gtatgtgatc
     9721 accacaaata tcacatctgg actagttccg tcttcatgcg caggtagact ggccccctga
     9781 atctccagac aaccag
//
LOCUS       DAZPAB010000141         9774 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZPAB010000141 DAZPAB010000000
VERSION     DAZPAB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.141"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            392..2023
                     /gene="oppA"
                     /locus_tag="ACHUPG_003015"
     CDS             392..2023
                     /gene="oppA"
                     /locus_tag="ACHUPG_003015"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR0862537.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2109..3029
                     /gene="oppB"
                     /locus_tag="ACHUPG_003016"
     CDS             2109..3029
                     /gene="oppB"
                     /locus_tag="ACHUPG_003016"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR0862538.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3044..3952
                     /gene="oppC"
                     /locus_tag="ACHUPG_003017"
     CDS             3044..3952
                     /gene="oppC"
                     /locus_tag="ACHUPG_003017"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR0862539.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3964..4977
                     /gene="oppD"
                     /locus_tag="ACHUPG_003018"
     CDS             3964..4977
                     /gene="oppD"
                     /locus_tag="ACHUPG_003018"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR0862540.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4974..5978
                     /gene="oppF"
                     /locus_tag="ACHUPG_003019"
     CDS             4974..5978
                     /gene="oppF"
                     /locus_tag="ACHUPG_003019"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR0862541.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6031..6360)
                     /locus_tag="ACHUPG_003020"
     CDS             complement(6031..6360)
                     /locus_tag="ACHUPG_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR0862542.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6395..7855)
                     /gene="cls"
                     /locus_tag="ACHUPG_003021"
     CDS             complement(6395..7855)
                     /gene="cls"
                     /locus_tag="ACHUPG_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR0862543.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7998..8171
                     /locus_tag="ACHUPG_003022"
     CDS             7998..8171
                     /locus_tag="ACHUPG_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR0862544.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8226..9479)
                     /gene="kch"
                     /locus_tag="ACHUPG_003023"
     CDS             complement(8226..9479)
                     /gene="kch"
                     /locus_tag="ACHUPG_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR0862545.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2563 a         2286 c         2433 g         2492 t
ORIGIN      
        1 tgaaagatgt ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga
       61 gaatcgctta acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc
      121 gggcaaccgc cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg
      181 cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc
      241 tgaatcacca gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca
      301 gcagaaagtc tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac
      361 cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt
      421 agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc
      481 cgcaggcgtc acactggcgg aaaaacaaac actggtacgt aacaatggtt cagaagttca
      541 gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt
      601 tgaaggctta ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg
      661 ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga
      721 tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa
      781 cactgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat
      841 tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt
      901 agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac
      961 ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa
     1021 tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct
     1081 tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt
     1141 gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac
     1201 gtataacaac atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt
     1261 tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga aaccgccatt
     1321 caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata tcattgttaa
     1381 taaagtgaaa gcgcagggcg acatgcccgc ctatggttat actccaccgt atactgatgg
     1441 cgcaaaattg actcagccag aatggtttgg ctggagccag gaaaaacgta acgaagaagc
     1501 gaaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgcg
     1561 gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa
     1621 aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg
     1681 tcaccagggc acttttgatg tggcccgtgc aggctggtgt gcagactaca acgaaccaac
     1741 ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc
     1801 ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc
     1861 tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta
     1921 ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca aagatccgct
     1981 ggataatacc tatacccgga atatgtacat tgtgaagcac taatggcaat acgtggggca
     2041 ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggcac aggccagaag
     2101 gtagggcaat gttaaaattt attctacgtc gttgtctgga agcgattccg acgctattta
     2161 ttcttattac tatttcgttc tttatgatgc gcctcgcacc gggaagccct tttaccggcg
     2221 aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat cttaatgatc
     2281 cgatcatgac acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat
     2341 cgtttaaata taaagattat tcggtcaatg acctggtggc atccagtttt cccgtttctg
     2401 ccaaactggg agccgccgca tttttccttg cggtaatact gggtgttagt gctggcgtta
     2461 ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg
     2521 gggttgttat ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt
     2581 tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga
     2641 tggtggcgtt gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga
     2701 ttgaagtatt acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc
     2761 ggatcatttt acgccatgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc
     2821 ccgcatttgt cggcattatt accggttcta tggtcatcga aaccatttat ggtttgccgg
     2881 ggattgggca actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc
     2941 tgaccatcct ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg
     3001 cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa
     3061 aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg
     3121 gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct
     3181 ggtgctgatc gcgttatttg taatcctggc accgatgctt tcccagtttg cctatgacga
     3241 tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac
     3301 tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat
     3361 ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc
     3421 cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa tcctcaactc
     3481 cttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggccaaa acatcctgct
     3541 gattttcgtg gcgattggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca
     3601 aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac
     3661 gccgggcatt gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc
     3721 atcgctactg gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg
     3781 tacgcaagag ccgttaagca gctggggggc attgctgagt gatggcgcga actcgatgga
     3841 agtctctcca tggttactgt tgttcccagc gggattcctt gtggtgacac tgttttgttt
     3901 caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca
     3961 gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg
     4021 ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc
     4081 aatgatttaa atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt
     4141 tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt
     4201 ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat
     4261 aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc
     4321 tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa
     4381 gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt
     4441 aaacgcatga aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agtcatgatt
     4501 gcgatggcat tgctatgtcg acctaagttg ctgattgcgg atgaacccac tacagcgctg
     4561 gacgtcaccg tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat
     4621 actgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg
     4681 ctggtaatgt acgccgggcg cacgatggaa tatggcaatg cgcgcgatgt cttttatcaa
     4741 cccgttcatc cttattctat cggtttgctc aacgcggtgc cgcgtcttga tgcggaaggt
     4801 gaaacaatgt tgaccatccc gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc
     4861 ccgttccagc cacgttgtcc acatgcgatg gaaatttgta gtagcgcacc gccgctggaa
     4921 gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg
     4981 ctgtaactga aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg cactttgaaa
     5041 tcaaagatgg caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg
     5101 tcactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta
     5161 agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt catgttgcct
     5221 ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata
     5281 ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga
     5341 tcatcgctga accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc
     5401 gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc
     5461 atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt attcttgaac
     5521 cgaagctgat tatctgcgat gagccggttt cggcgctgga cgtgtcaatt caggcgcagg
     5581 tggttaacct gctccagcag ctgcaacgtg agatgggatt gtcattaatt tttattgctc
     5641 atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg
     5701 cagtagaact ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg
     5761 cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt
     5821 tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc
     5881 gttgcccgat tgccggtccg gagtgcgcca aaacacgtcc tgttctggag gggagtttca
     5941 gacacgccgt ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg ctgacagttg
     6001 tcagcccttt ttcacgctaa aagcgattat ttattcccgc cagatgatat ggcaaagttt
     6061 gtgatctttt tcgcggcata acagaatgcg ggcgaaaaca tcgctgattt caccatcttc
     6121 actgtccgcc agaccaatca ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac
     6181 atgttcctgc cagtcttccg ccggatcgaa taactccgcg ccgccgcgct cttcaaactg
     6241 aagattgaac agcagaacat cagctggatc gagattgtcg gcagccagtt cgagaaaaat
     6301 atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga ttgtttagat ccatatccat
     6361 agtcactacc tgtttaacct ctgttggcga cgttttacag caacgggctg aagaagtaaa
     6421 acagtcgctc ggcgacacgt tgccataatg gacgttttag ccataaacgg gcatcgagca
     6481 gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca aaacctttat
     6541 cgtcgattgc caaggtaatc tcgaaattca gccacagact acgcatatca aggttaacgg
     6601 tgccaaccag acttagttcg ccatcgacca gcacgctctt ggtatgcagt aacccgcctt
     6661 caaactgata aattttaacc ccagcagcaa gcagttccgt aaagaatgcg cgactggccc
     6721 agccgaccag catcgagtca ttttttcgcg gaaggataat actgacatcc accccgcgct
     6781 gcgccgccgt gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca
     6841 tgatcaaata ttcatgcgcc gaataagccg cagtcaataa tgcctggtga atgagatctt
     6901 ccggaaagcc ggggccagaa gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg
     6961 gcataatatt gacatctggt ggtggcggca gaatacgttt tccggtttca atctcccagt
     7021 cgcaggaata aataatcccc atcgcggtgg cgatggggcc ttccatacgc gccatcagat
     7081 caatccattg ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc atattcatgc
     7141 tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca
     7201 tacggcgtaa aaacacacgc atcagattga cctttaaggc ttcgaccact tcaataccgg
     7261 cattacgcat tagctcgggc cacgggctgc ggaaaaaagc cacactcccg gcggagtcga
     7321 gcatcaatcg gcaatgaata ccacgccgtg cagccgccat taatgattca gccacctggt
     7381 ccgccatgcc gccgggctgc cagatataaa acaccatctc aatattatgg cgcgcgagct
     7441 ggatgtcgcg gattaacgcc tgcatcacat catctgactc ggtcatcagc tgtagctgat
     7501 tccctttgac cccagcgatc ccctgacgac gctcgcaaag cttgaataat ggcgcagcga
     7561 cactgctatt ttcttcggcg aagatatgct tacaggcttt aaggtcgtta agccattttg
     7621 cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc
     7681 caacggcaag ataggcaata attccgacta acggcagaat gtaaataatc aacagccagg
     7741 ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg taaagttacg cctgcaatga
     7801 gcaaccagta tcccagaatg gccaaccaac tcaccaacgt ataaacggtt gtcatagatt
     7861 agaaatcctt ttgaaagcgc attgttatga gtttacgcat atggattcat ctggcaaata
     7921 aaaacgcggg aaaagcactg gtcagcgtcg ggggcggggg tataatgacc attctgttat
     7981 tgcatagagt agttaacatg aagcggagta gaacggaagt ggggcgctgg cgcatgcagc
     8041 gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga aatatgcgta
     8101 tccacagcat caggaagtgc attctaaaca aacagcgtaa ctcgttattg tttgcgatct
     8161 acaatatcta aatgtactgg gcaccattgc ggtgcccaat aagtttactc ttccgtcttt
     8221 aaactctatt tttgcgccga ttctttactg tcagctttcg tttcttgttc atcgttatcg
     8281 ctgaaaatac catgacctga gttcaacaac attgaaacga gcatatcatt attaatctct
     8341 tcaccattta aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga gagaatgata
     8401 tccggatgta ccatcttaat cttattcagg tttttgctat cactgacggc gagaactgtt
     8461 ttgacatcac tgctcatatc ttttgccgag agtacaacaa acgcgttatc tgcatcgtta
     8521 tcactcagcg ccagaatggc ccggcaacga tcgattcccg ctttctttaa tactgaactg
     8581 tcattactgt caccggggat aacatcagcg ttatcgccta agcgttgctc aagttgcttg
     8641 atatcatctt caggcaagtt gctgataacc gttacgtttt gtccgcgttg attcagttgc
     8701 aggatcgtat tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc
     8761 attgtatgat tgtttccttt taaaagtttg ttgaatcccc cgcggataag cggaccaaaa
     8821 attgaggtca tagatgtggc aaaaacggta atgccggaaa taatgaccga aatagtgaac
     8881 aatcgtgctg attcagaaac agggacaata tctccatagc cgacggttga catggtttct
     8941 atcgaaaaat agaacgcggt catcaaactt tctattcgcg gattaaaacc ttcgcttaaa
     9001 taaagcgcac cgtaggttga gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt
     9061 gtcccggctg cggcgctact gtgggagaag tctttgcgca gtatcagcaa aaacaccagc
     9121 gtaaaaatgc aaaatccaat actaaatttc agccagggat aaaaatgcag ggtgtaaatt
     9181 agcgctatca acaacaaaat gatactgatt gcccatgcga gctttgcccg gaacagtaag
     9241 ccaatggagt tcaggacaag aaagacgccg agcataaata gtggggcgtt tgccagcaag
     9301 gagagatcca actctgaaaa tgctttgata tggaaaatat caagcaaatt gaccgacata
     9361 ccatagattg ttttaaaaat aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg
     9421 tgccgtaggg tcacccataa atttgttgct gtttgtttga atgtagccca gtgactcact
     9481 gatacctctg taaccacaat tttgtttaat tatcttagcg tgcacatttt ctgaacatac
     9541 atgcagcgcg tgaatgtgtt aaacaatagt agatgacttt atccgcagta atcaaaggtt
     9601 ttgagtgagc aactctgcgt caggaaaggg cttttttcct gaacatgctc acatcatata
     9661 aagaaataca gataaagtta ttatctgctt gtggtagtga atgcactgac cggctataag
     9721 gaaaggccaa acaagaacac ggttgcaaaa accgtgccct taaatattgg atct
//
LOCUS       DAZPAB010000142         9755 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZPAB010000142 DAZPAB010000000
VERSION     DAZPAB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.142"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(119..1459)
                     /gene="gudD"
                     /locus_tag="ACHUPG_003024"
     CDS             complement(119..1459)
                     /gene="gudD"
                     /locus_tag="ACHUPG_003024"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR0862546.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(1480..2820)
                     /locus_tag="ACHUPG_003025"
     CDS             complement(1480..2820)
                     /locus_tag="ACHUPG_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR0862547.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(2822..4174)
                     /gene="gudP"
                     /locus_tag="ACHUPG_003026"
     CDS             complement(2822..4174)
                     /gene="gudP"
                     /locus_tag="ACHUPG_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR0862548.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(4608..5057)
                     /locus_tag="ACHUPG_003027"
     CDS             complement(4608..5057)
                     /locus_tag="ACHUPG_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR0862549.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(5075..5857)
                     /gene="truC"
                     /locus_tag="ACHUPG_003028"
     CDS             complement(5075..5857)
                     /gene="truC"
                     /locus_tag="ACHUPG_003028"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR0862550.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(5857..6186)
                     /locus_tag="ACHUPG_003029"
     CDS             complement(5857..6186)
                     /locus_tag="ACHUPG_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR0862551.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(6808..7353)
                     /gene="syd"
                     /locus_tag="ACHUPG_003030"
     CDS             complement(6808..7353)
                     /gene="syd"
                     /locus_tag="ACHUPG_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR0862552.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            7421..8269
                     /gene="queF"
                     /locus_tag="ACHUPG_003031"
     CDS             7421..8269
                     /gene="queF"
                     /locus_tag="ACHUPG_003031"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR0862553.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            8381..9745
                     /gene="ppnN"
                     /locus_tag="ACHUPG_003032"
     CDS             8381..9745
                     /gene="ppnN"
                     /locus_tag="ACHUPG_003032"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR0862554.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         2434 a         2661 c         2300 g         2360 t
ORIGIN      
        1 caattaaatt caatgaatta aaaaatgatg tagacctgat acatagcaca acaggctatt
       61 ttgcgtttag catcagtctc aaaccggctc cagatagagc cggttttggt tttctgtctt
      121 aacgcaccat gcacgggcgc ttgttatcga acgtccagcc aggaatcaga tactgcattc
      181 ccatcgcatc gtcacgcgcg ccaagcccgt gtttctgata cagctcatgg gctttcatca
      241 cttgatccat atcgatttct acacccagcc ccggtttttc tggcacctgt accagcccgc
      301 ctttgatctc aaacggttct ttggtcaggc gctgattacc ttcctgccag atccagtgcg
      361 tatcaatagc agtaatttta cccggtgcag cggcggcaac atgggtaaac atcgccaggg
      421 aaatatcgaa gtggttgttt gagtgtgaac cccaggtcag gccaaattca tggcacattt
      481 gcgccacacg taccgaacct tgcatagtcc agaaatgcgg atccgccagc gggatatcaa
      541 cggattgtaa cgagagcgta tggcccatct gccgccagtc ggtggcgatc atattggttg
      601 cagtcggcag gccagtcgcg cgacggaact ctgccatcac ttcacgcccg gagaaacctt
      661 gctccgcacc acacggatct tctgcataag ccagcgaacc tttcaggtat ttaccaattt
      721 taattgcttc gttcagcgac caggcaccgt taggatcgag cgtaatacgc gcctgcggga
      781 agcgttgcgc cagtgccaca atagactcgg cttcttcttc cccggccagt acaccgcctt
      841 tcagtttgaa atcgttgaag ccatattttt catacgccgc ttccgccagg cgcaccaccg
      901 catccggcgt catcgcttct tcatgacgca ggcgatacca gtcgcatgag tcatccggct
      961 ggctttgata cggcagaggt gtggctttgc gattaccgac gaagaacaga taaccgagca
     1021 tttcgacttc gctacgctgt tgaccatcgc ccagcagcga tgccacgttt acccccagat
     1081 gctgccccag cagatccagc attgccgctt ctatcccggt aactacatga atagtggtac
     1141 gcaaatcaaa tgtctgcaaa ccgcgcccac tagcatcacg atcggcaaaa gtattacgca
     1201 ccagcgtcag aacgtttttg tattcaccca gcgttttacc taccaccagc ggaatcgcat
     1261 cttccagcgt tttacggatt ttctcgccgc cgggaatttc ccctacgcca gtgtgaccgg
     1321 aattatcttt gataatcaca atattacgcg tgaagaacgg tgcgtgtgca ccactcagat
     1381 tcatcagcat actgtcatga cccgccaccg ggatgacctg catttcagta acaacaggcg
     1441 tcgtaaattg agaactcata tttatgtcct ttttcagaat caatgacggc cgaaaacggg
     1501 acgtttacgg tcaaaggtcc agccggggat caggtactgc atcggacctg cgtcgttacg
     1561 cgcaccgcaa ggcagacgtt tataggcctc atgtgccttt tgtacctgtt cccagtccag
     1621 ttccacgccc agaccgggcg catcaggaac ggcaattttt ccgtttttaa tctccagcgg
     1681 atttttggtc aggcgacaat cgccctcctg ccaaatccag tgggtatcga tagcggtagg
     1741 attacccggt gccgccgcgc ccacatgggt aaatatcgcc agagagatat cgaaatggtt
     1801 gttggaatgg cagccccagg tcagccccca gtcgtcgcaa agctgcgcca cgcggactgc
     1861 accggaaagc gtccagaagt gcggatcggc aagtggaata tctaccgcat tgagcatcac
     1921 cgcatgaccc atttcgcgcc agttggtggc gatcatgtta gtcgcgacgg gcaagccggt
     1981 cgcccgtcga aactccgcca tcacttcacg tccggagaag ccctgttctg cgccgcatgg
     2041 atcttcggca taggtaagaa catcattcag ccctttgcat aaagaaatgg cttcatcaag
     2101 cagccatgca ccgttgggat caacggtaat ccgcgcatcc gggaagcttt tcttcaatgc
     2161 acgaacagtg tcgatttctt gctcgccagg taacaccccg cccttaagtt tgaaatcttt
     2221 aaagccatag cgatcctgtg aggcttccgc cagacgcaca acggcttcgc tgttcatcgc
     2281 tttctgatgg cgcaactgat accactcatg gttgcccggc gtattttcca gataaggaag
     2341 atcggttttg gtccgatcac ctatataaaa cagataaccg aggacggtaa tagtctcgcg
     2401 ttgcttgcct ggccccaaca gttcgcagac cggaacattc agcgccttac ctagcaggtc
     2461 aagcaaggcg gcttccagcg ccgccacggc attaacgcgc aattcaaaag tccaggcacc
     2521 tttgccaaag gtatcaaaat cggctgcctg attaccttta tgtacctgct gaaccacttt
     2581 attcaggcgc gcaacttcct ggcccagaac catcggaata gcatcgacca gtgtttgata
     2641 aatcacctct ccgcccggcg cttcaccaat gccggtatgc ccggcgttat cggtgagtac
     2701 cacgatattg cgggtgaagt atgcgttatg cgcaccacca atattgagca acatgctgtc
     2761 atgtccggcg accggaatga ctttcatatc agtaataaca ggactggatt gtgtcgccat
     2821 cttattgccc cgcgacaggt ttcagctcga tacgcttgat atcgcccacc agcaccaggt
     2881 agctcagtac cgcgattagg gcatgaacac caacataaat cagcgcccca ttaaacgagc
     2941 cagtcgtgcc aacgatataa ccaattgcga ttggcgtgac gatgccagaa atgttaccga
     3001 acatgttgaa caggccaccg gaaagaccgc tgatctcttt tggcgcggta tctgccatta
     3061 ctgcccaacc cagcgccccg atgcccttac cgaagaaggc cagcgccata aagccgatga
     3121 tcatccactc aacgttgacg tagttgcaga acaccatcac catcgacagc aacatgccca
     3181 ttacgatcgg tgttttacgc gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg
     3241 aaataattcc acccagcaca ccgccgataa aaccgcaaac cgccggaacg gaagccacaa
     3301 agcccgcttt cagaatcgac atcccacgcg cctgcaccag ataaaccgga aaccaggtaa
     3361 tgaaaaagta agtcagggcg ttgatacagt actgaccgat ataaacgccg atcatcatcc
     3421 gtgacccgag cagctgtttg atctgccccc acttcacgct gaacggcact ttaactttgg
     3481 tgctttgctg atccatattg atcagcgcgc cacccgcggc gatatactcc agctctttct
     3541 gatttacccc cggatgttga tttggctcgt ggatgacttt caaccagata aagctgatga
     3601 caatgcccag accgcccata aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc
     3661 agcccataat cggcgcgaag atcaccgttg cgaagtattg agcggagtta aaaatcgaca
     3721 ccgccgttcc cctttcctgc accggaaacc aggccgcaac aatgcgactg ttgccgggga
     3781 aagatggcgc ttcagcaagc ccgaccagga agcgcagagt aaacagggca acgataatgc
     3841 cgaatccact aaagatatcg acgaagcctt gcagcaaggt aaacatcgac cagataaaga
     3901 tcgaccagaa gtagacgcgt tttgaaccaa aacgatccag caaccaacca ccagggatct
     3961 gcccgataac ataagcccat gagaaggcag agaacacata gcccattccc acgggatcaa
     4021 ggccgatatc tttggccatt tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt
     4081 tgaaggatgt gacgataaac aacatcacca ctatccagta acgagcattc gtgcgttttt
     4141 ccacactgct cgcagcctga cttaaagaac tcattgttgc actcctgaaa attcgcgtta
     4201 gccacgctca ctctggactg cgacatcgcc aggaaatcag aggtgacgta gggtgttttt
     4261 tgccgttttt ataggtcgtt cgccgaatac ggcgcgtgtt tatatctggc aatagcagta
     4321 taaaaagcgc gccatagcgg ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg
     4381 cccgattttg gcattagccc tggacggtgg aatccacttc acggaaatga aaacaagaac
     4441 aagaaaggaa gggtaaaaac gaagaaataa agaagagtat gaaatggatc gcttgactct
     4501 aggcaaacgc cagtaaaaat ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa
     4561 gattcgattc agcaccgcta aaacgacatt taccgctcgc tgacatatca ggacaacagc
     4621 gtgccccact gttcgaccca cggatttgat tccgtttccg gttccgggtt ctcactggcg
     4681 tcaatcaaca gcatttcgcc aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac
     4741 tgtttgccgc cattgcaaaa atttacataa ctgctgtcgc cgagcgcaat cacgccataa
     4801 cgcagattcg gctggaagcc cagactatct ttgattccct gaaagagcgg cacaatgcta
     4861 tcaggaaggt cgccctgccc ggtcgtggac gtaaccacca gaacatactt atcctgatag
     4921 ggcagccagt cgcttaattc aggatcttca aataccgttg ctttgtggcc ctgtgcggtc
     4981 agaattgctt ctgcttcttc cgccactaac agcgaatttc cgtacatggt gccgacaaaa
     5041 ataccaattt ccgccatacc gttttctccc tggattagga acttatctct ccatcctgac
     5101 ccgatggcgc actgaactca accctttcat tttcaggaag cagaccgcgc cagccaaatt
     5161 gtgataacgc ctgcatccag gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag
     5221 taaaaggatg cgtcaatgac aactgactgg catgtagcat taaccgctgg aggccaaaat
     5281 gctcagcacc gctgcgattc tggcgtaaat cgccatgttt gctatcgcca ataatcggat
     5341 gacgcaaatg ggcaagatgt cgtcgaagct gatgtttgcg tccggttttc ggctccagtt
     5401 ccaccaggcc gtagcgcgtg gtcgggtaac gtccggtcgc taccggcatt tctacggtcg
     5461 ccagaccgcg ataatgcgtc actgctggct gcgggccttt atcttcgcgg gcaaatttat
     5521 cagcgatttt gtccagttct tccaccagtg gataatccaa caccgcttct tccatcaacc
     5581 agccgcgcac aatcgcatgg taacgtttct ggatttggtg ctgttcaaac tgttgtgcca
     5641 gcagccgtcc ggcctcgctg gataatccca tcaacaacac accagaagtg ggtcggtcca
     5701 gacgatgagc agtaaaaaca tgctgtccta tctggtcacg cacggtttgc atgaccacta
     5761 ctttctcgtc gcgatccagc cagctacggt gaaccagcca gccggagggt ttatttaccg
     5821 caaccagcca ttcatcctga tagagtattt ccagcattag ctcgcatcat ccgcaaaaag
     5881 agcatccagt ttttccaact cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag
     5941 cgccatttca taatagggtg caacagcaaa agcgcccggt aacggctggt tgttatttaa
     6001 caaatcgtgc attcgcggga tcagccccca ctgcaaccac tccagtggtt ccatggtgtc
     6061 cataaagaac ggttgggtac tattaaattg atgcggctgg ggttcatcgt ttcgccagtg
     6121 ctgatgttca cgcagtaacg cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt
     6181 ggtcataaag caacctcaat aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg
     6241 gagcactgta ttcacagtgc tcccggttcg tttcgcagca ttccagctac ttttgttgct
     6301 ccctgctcat ccttgacaac ttttcctctg gccttgcggc caatcgttca tcctgaacta
     6361 ttgcttcctg ctcacaccac cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc
     6421 tgacccaccg aatcatcctg accggttctc attctccatc ctggaggtgt cctttaacgc
     6481 gtcctgcgtc atcctcttcg cttcatccag aagcctttcc ctgaaacacc atcctggtgt
     6541 gtcctgcaga agtgtcatca tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa
     6601 gatagaatcg tctttttcgc gaagtcttac aaggcgctta cagacaatgc cttaaggaaa
     6661 atttcatatg aaagttaaga attaacaatc aacattttga ttttaaatga aaataacgat
     6721 tcttatcttt ttaagtattc gcttatttcc tggcgatctc ctacagagtc tgtaagagat
     6781 ctctcacaac cagatggggt atttggatta cagaagaggc ttaagttgat taaggaattc
     6841 cgcaagattt gaggctaaat tcgtccgttt acgtgttccc agcgtctctt tgcacacttc
     6901 accggagaga ttacacaccg agatgacctc taactcttct tccagcgtcg cgataaatag
     6961 cgtgggcggc agttttagcc ttttctgagt caccagatga ccaatcagat tttcctgcac
     7021 ccgacggaag tcgtcttcac tccaggtttg cagcaatgtc agtttgatat cgccaaactg
     7081 cgcgtgcata tccccggcaa actgagtcgt ataaaaggta tgaattgtgg gttgtatcac
     7141 aatatcaaaa gcgcgttcaa ccgcgtttac attttgttcc ccggtaaacg gctggggttg
     7201 ccagtatacg gcatcttcgg tagttgaaat aatgcacggt gaaggaacac cgtacaattc
     7261 ctcacttaac ggccagcttt tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa
     7321 atctttcagg gcttgtgcgg tcaaatcgtc cactaatttc tctcttcact taaaccagat
     7381 acacttgtcg tttagtttat ctggtttatg acggtgaaac atgtcttctt atgcaaacca
     7441 tcaggcactt gcgggcctga ctcttggaaa atcaaccgat taccgggata cctatgacgc
     7501 cagcctactg caaggcgttc cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga
     7561 taacctgcct tttcacggta cggatatctg gacgctgtat gaactttcct ggctgaatgc
     7621 gaaaggtttg ccgcaggtcg ctgtcggtca tgttgaactt gattacacca gcgtaaatct
     7681 gattgagtcg aagagtttta agctctatct caacagtttt aaccagacgc gttttaataa
     7741 ctgggatgag gtgcgccaga cgctggagcg cgacttaagc acttgcgctc agggtaagat
     7801 tagcgtggcg ttatatcgtc ttgatgaact ggaaggccag ccgataggtc atttcaatgg
     7861 cacttgcatt gatgaccagg atatcactat cgataactat gaattcacta ctgactatct
     7921 ggagaatgcc accagcggtg aaaaagtagt ggaagagacg ctcgtcagcc acctgctgaa
     7981 atcaaactgc ctgatcaccc atcaaccaga ttggggttcg atccaaattc agtatcgtgg
     8041 acgccaaatt gacagagaaa aactgctgcg ttacctggtc tcattccgtc atcacaacga
     8101 gttccacgaa cagtgcgtgg aacgcatctt taatgacctg ttacgcttct gccagccaga
     8161 gaaattgagc gtttacgcac gttatacccg tcgtggcggt ctggacatta acccgtggcg
     8221 cagtaatagc gattttgtcc catcgaccac aagactggtt cggcaataaa ttttttctca
     8281 attttgcgtg ctggattcac gcagaaggtt gtgaaaggtc atcaggcagg gctattgtaa
     8341 tcaaagggaa tgacgatatt cgtcccataa ggagtttttc ttgattacac atattagccc
     8401 gcttggctcc atggatatgt tgtcgcagct ggaagtggat atgcttaaac gcaccgccag
     8461 cagcgacctc tatcaactgt ttcgcaactg ttcacttgcc gtactgaact ccggtagttt
     8521 gaccgataac agcaaagaat tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg
     8581 ccgtgaacgc ggcgtaaagc tggaactgat taatcccccg gaagaggctt ttgtcgatgg
     8641 gcgaattatt cgcgctttgc aggccaactt gttcgcggtt ctgcgagaca ttctcttcgt
     8701 ttacgggcaa atccatcata ccgttcgttt tcccaacctg aatctcgaca actccgtcca
     8761 catcactaac ctggtctttt ccatcttgcg taacgctcgc gcgctgcatg tgggtgaagc
     8821 gccaaatatg gtggtctgct ggggcggtca ctcaattaac gaaaacgagt atttgtatgc
     8881 ccgtcgcgtc gggaaccagc tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc
     8941 aggagcgatg gaagcgccga tgaaaggtgc tgcggtcgga cacgcgcagc agcgttacaa
     9001 agacagtcgt tttattggta tgacagagcc gtcgattatc gccgctgaac cgcctaaccc
     9061 actggtcaac gaattgatca tcatgccgga tatcgaaaaa cgtctggaag cgtttgtccg
     9121 tatcgctcac ggcatcatta tcttccctgg cggtgtgggt acggcagaag agttgctgta
     9181 tttgctggga attttaatga acccggccaa caaagatcag gttttaccat tgatcctcac
     9241 cggcccgaaa gagagcgccg actacttccg cgtactggac gagtttgtcg tacatacgct
     9301 gggcgaaaac gcgcgccgcc attaccgcat catcattgat gacgccgctg aagtcgctcg
     9361 tcagatgaaa aaatcgatgc cgctggtgaa agaaaatcgc cgtgatacag gcgatgccta
     9421 cagctttaac tggtcaatgc gcattgcgcc agatttgcaa atgccgtttg agccgtctca
     9481 cgagaatatg gctaatctga agctttaccc ggatcaacct gttgaagtgc tggctgccga
     9541 cctgcgccgt gcgttctccg gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc
     9601 cattgaagag tttggtcctt acaaaatcaa cggcgataaa gagattatgc gtcgtatgga
     9661 cgacctgcta cagggttttg ttgcccagca tcgtatgaag ttgccaggct cagcctacat
     9721 cccttgctac gaaatctgca cgtaatctcc cctcc
//
LOCUS       DAZPAB010000143         9729 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZPAB010000143 DAZPAB010000000
VERSION     DAZPAB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9729
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.143"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1..1457
                     /gene="citT"
                     /locus_tag="ACHUPG_003033"
                     /pseudo
     CDS             1..1457
                     /gene="citT"
                     /locus_tag="ACHUPG_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            1571..2377
                     /gene="rna"
                     /locus_tag="ACHUPG_003034"
     CDS             1571..2377
                     /gene="rna"
                     /locus_tag="ACHUPG_003034"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR0862555.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            2606..3016
                     /gene="rnk"
                     /locus_tag="ACHUPG_003035"
     CDS             2606..3016
                     /gene="rnk"
                     /locus_tag="ACHUPG_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR0862556.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            3154..3243
                     /gene="yldA"
                     /locus_tag="ACHUPG_003036"
     CDS             3154..3243
                     /gene="yldA"
                     /locus_tag="ACHUPG_003036"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR0862557.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(3247..4456)
                     /locus_tag="ACHUPG_003037"
                     /pseudo
     CDS             complement(3247..4456)
                     /locus_tag="ACHUPG_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
     gene            4677..5105
                     /gene="uspG"
                     /locus_tag="ACHUPG_003038"
     CDS             4677..5105
                     /gene="uspG"
                     /locus_tag="ACHUPG_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR0862558.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(5226..6791)
                     /gene="ahpF"
                     /locus_tag="ACHUPG_003039"
     CDS             complement(5226..6791)
                     /gene="ahpF"
                     /locus_tag="ACHUPG_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR0862559.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(7036..7599)
                     /gene="ahpC"
                     /locus_tag="ACHUPG_003040"
     CDS             complement(7036..7599)
                     /gene="ahpC"
                     /locus_tag="ACHUPG_003040"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR0862560.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            7971..8717
                     /gene="dsbG"
                     /locus_tag="ACHUPG_003041"
     CDS             7971..8717
                     /gene="dsbG"
                     /locus_tag="ACHUPG_003041"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFR0862561.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            8927..>9729
                     /locus_tag="ACHUPG_003042"
     CDS             8927..>9729
                     /locus_tag="ACHUPG_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0862562.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLE"
BASE COUNT         2457 a         2420 c         2310 g         2542 t
ORIGIN      
        1 ttagcaaaag ataatatatg gaaactattg gccccactgg tggtgatggg tgtcatgttt
       61 cttatccctg tccccgacgg tatgccgccg caggcatggc attacttcgc tgtgtttgtg
      121 gcaatgattg tcggcatgat cctcgagcca attccggcaa cagcgatcag ttttattgcg
      181 gttactattt gcgttattgg cagtaattac ctgctctttg atgccaaaga attagctgac
      241 ccagcgttta atgcgcaaaa acaggcgctg aaatggggcc tggctggttt ttccagcacc
      301 acggtatggc tggtatttgg cgcatttatt tttgcattag ggtatgaagt ttccgggtta
      361 ggtcgtcgca ttgccctttt cctggtgaaa ttcatggaca aacgcacgct gacgttgggt
      421 tatgcgattg tcattatcga cattctgctg gcaccgttta caccgtccaa caccgcgcgt
      481 accgggggta cggtttttcc ggtcattaaa aacctgccgc cgttgtttaa atcattcccg
      541 aacgatccgt ccgcgcgtcg tattggcggc tatttgatgt ggatgatggt cattagtacc
      601 agtctgagtt cgtccatgtt tgtcaccggt gcggaccaaa cgtgctgggt ctggagttcg
      661 tcagcaaaat tgccggtatc cagattagct ggttgcagtg gttcctctgc ttcctgccgg
      721 ttggggttat cttgcttatc attgcgccgt ggctttccta cgtgctgtac aaaccggaaa
      781 tcacacacag tgaagaagtg gcaacctggg cgggtgatga actaaaaacc atgggtgcgc
      841 tgacacgcag agagtggacg ctgattggcc ttgtattgct cagcttaggt ttgtgggtat
      901 ttggcagtga agtcattaat gctactgcgg ttggtctgct ggcagtttcg ctaatgctgg
      961 ctctgcacgt tgtgccgtgg aaagacatta cccgctataa cagcgcatgg aacacgctgg
     1021 tcaacctggc aactctggtt gtgatggcta acggcctgac tcgttctggt tttattgact
     1081 ggttcgccgg taccatgagt acgcacttgg aaggattctc accaaacgca acggtgattg
     1141 tactggttct ggtgttctac tttgcacact acctgtttgc cagcctgtct gcgcacaccg
     1201 caaccatgct gccggttatt ctggccgtcg gtaaaggtat tccgggcgta ccaatggaac
     1261 aactgtgtat cctgctggtg ctgtctatcg gtatcatggg ctgtctgacg ccgtatgcaa
     1321 ccggtcctgg ggtgattatt tacggctgtg gctatgtgaa atcaaaagat tactggcgtc
     1381 ttggcgcaat cttcggggtg atttacatct ctatgttgct gttggttggc tggccgattc
     1441 tcgccatgtg gaactaatac gttcgccaaa cgctgaataa atcatcacgc ccgccaggta
     1501 agcccacctg gcgggctttt ttatgattta atagatagta ttcagtgtat gtatgagttc
     1561 cacacccatt atgaaagcat tctgccgtaa cgccgcgttg ctcgcggttt ctctgcttcc
     1621 cttatcttct gccaacgccg tagcgttgca ggcaaaacag tatggcgatt ttgatcgcta
     1681 tgtcctggcc ctctcctggc aaaccggatt ttgccagagt caacacgatc gaaatcgtaa
     1741 cgaacgagat gaatgtcgcc tgcaaaccga aacgaccaac aaaactgatt ttctgaccgt
     1801 acatggtctg tggccaggat tgcctaaatc ggttgctgcc cgtggtgttg atgaacgccg
     1861 ctggatgcgc ttcggttgcg ctactcgccc aatcccgaat ctaccagaag cacgcgccag
     1921 ccgaatgtgt tcatcgccgg aaaccgggtt atcactggaa acggccgcta aactaagtga
     1981 agtcatgcca ggagctggcg gacgttcctg cctggaacgc tacgaatatg ccaaacacgg
     2041 tgcctgcttt ggttttgatc cggacgcata cttcggtacg atggtacgcc tgaatcaaga
     2101 aattaaagag agcgaagccg gaaaattcct tgcggataat tacggtaaaa cagtgagccg
     2161 ccgtgacttt gacgccgcct ttgccaaaag ctggggaaaa gagaacgtga aagcagttaa
     2221 gctaacgtgc cagggtaacc ctgcgtattt gactgaaatt cagatctcga tcaaagctga
     2281 cgccatcaac gctccgcttt ctgcaaactc attcttgcca caacctcacc caggtaactg
     2341 tggcaaaacc tttgtgattg ataaagcggg ttattaatcc tctgatttac aaaacacgct
     2401 aattcgtctt ccataaccat gccgattaag aattatacat aatgcgctgc attgaggaat
     2461 tccctgaatg tgacgcaaat cacttcaaag agtgagcaac agtcagtatc atcatgtgag
     2521 ttaaaccctc gccgcctgac ggtgagggtt ttcttttggg tatgtttatc tgcgacactc
     2581 gcagtaccga cgacatggag taaaaatgtc cagaccaact atcatcatta acgacctgga
     2641 tgccgaacgc atcgatattc tgctggagca acccgcctat gctggtttgc caatcgccga
     2701 cgcgttaaac gcagagttgg atcgcgccca aatgtgttcg ccagaagaga tgccacacga
     2761 cgtggtgaca atgaacagcc gggttaaatt ccgcaatctt agcgatggcg aagtgcgtgt
     2821 gcgcacgctg gtgtatccgg caaaaatgac cgatagcaat actcagcttt ccgttatggc
     2881 tccggtaggt gccgcactgc tggggctgcg cgttggcgat tccattcact gggaacttcc
     2941 gggcggcgtt gcaacccacc ttgaagtgct ggaactcgaa taccagccag aagctgctgg
     3001 cgactacctg ctttaatccc atctgcacaa gcgataactc gctttgccca gaggatgcac
     3061 tcgcatcctc tttacgcaaa atcagcaatt tttcctggtt ctcactgttc acttttgctg
     3121 caaaattatt ccgtttcgtt tgtgggaata accatggcag aagcatttta catcctgata
     3181 ggatttctga ttatggcggc gattatcgtc atggctgtcc tttacctcga aaaccactcc
     3241 tgacgatcat attgttcccc ccggcatcgc attcaccaga cctgaaaccg ccttctgcgc
     3301 cgcctctgcg ctttgtgcac cgggtaccag aatcacctta cggcactcct cttcacgttt
     3361 ttcgaaaatc tcatatcccc gggcggcctc ttcaaacggc atatagtggg taacaatttc
     3421 ttctggtttc agtaatcctt tctcaattaa cggtaataat tctcccagcc atgcgtgaac
     3481 gtgggtctgt cccattttaa acgtcaaccc tttatcaaag gcgtcgccaa acaggaaacc
     3541 gtgaataaat ccagcgtaga cgcccggtac gctaacaatg ccgcaacgct ttaccgctgc
     3601 tgccctccag tttcaggtta gtcagcaccg tttccgtggt gctgcctttc gcttcaaaac
     3661 cgacggcgtc tattactgca tccacgcccc ggtgacctgc cgtttgttca ataattgact
     3721 gtgccggatc gctatcttca tcgaaattaa tcgggatcgc gccgtagcgg tcagcggcga
     3781 aacgcaagcg gtagggatga tgatcaacaa caaaaatctg ttccgcaccg agcaaccgtg
     3841 cacaggcgat tgtcaacaat cccacaggac cagcaccata gactgcaacg cttgaacctt
     3901 gttggatctg cacatttttt gctgcctgcc atgccgttgg cagaatatca gaaaggaaaa
     3961 gggctttatc atctgaaagc aaaggcggta ctttaaacgg ccccacattc cctttcggga
     4021 cgcggacata ttccgcctgc ccgccaggaa cgccaccata caggtgacta taaccaaaca
     4081 atgccgcggg agcaggtatc tgttttttat tgagcgcagc gcctttaccc gcattggtat
     4141 tttcgcaggc ggcatattgc tgtaatcgac agaaaaaaca gtcgccacaa gcaatgacga
     4201 acggaattac cacccggtcg cctttttgca aattttttac gtccttcccg gtttcaacga
     4261 cttcccccat aaattcatga ccaaaaatat cgccatgttt aacctggggt attttgcctc
     4321 gataaagatg gaggtcagag ccacagatcg ccgttaccgt aatacgcaga ataatatcat
     4381 ctgcctgttc aatgcccgga tcgggaacat tttctacctg aacgtgatgt gggccgtgat
     4441 aagtcaatgc tttcatgcaa cctccagaga cagttcaaaa aagagtcagt ctttaagggt
     4501 agctatcaca gcgggaaaac gggcggtacc gcaggttttg ggagaattcc tagtgttcag
     4561 tgagaatata ttaatcacag aaaccgttcg taaggaaaaa gaaccagaca aatatcaggg
     4621 ataaatatta taatcatgga gaatgatttg ttcatgatta acagggagaa agggttatgt
     4681 ataaggcaat cattatgcca gttgatgtat ttgaaatgga attgagcgac aaagctgttc
     4741 gccacgctga attcctcgcc caggatgacg gagttattca tctacttcac gtactacccg
     4801 ggtcagccag cctgagcctg caccgttttg ccgctgatgt gcgtcgtttt gaagagcatc
     4861 tgcaacatga agcagaagaa cgtctgcaaa cgatggtcag ccacttcacc atcgatcctt
     4921 cccgcattaa acaacatgtc cgttttggta gcgtgcggga tgaagtcaat gagttggcag
     4981 aagaactggg ggctgatgtt gtagttatcg gttctcgcaa cccatcgatt tcgacccatc
     5041 tgttaggttc taacgcctcg agcgtgatcc gccacgccaa tctgcctgta ctggtcgttc
     5101 gctaacagat aaaagtattt atgccgccct gcctggaaca tcccggcagg gctttttaat
     5161 gccataaaaa agccgccagg tttgaccccg gcggctaagc aattgcaggt gaatcttact
     5221 tcttcttatg cagttttggt gcgaatcagg tagtcaaaag cactcagaga ggctttggca
     5281 ccttcgccag tggcgatgat gatctgcttg tacggaaccg tcgtacagtc acccgctgcg
     5341 aacacgcctt tgacgttggt ttcacatttc gcatcaatga taatctcgcc catgcggtta
     5401 cgttcgactg cgccttccag ccagttggtg ttcggcagca gaccaatctg gacaaaaata
     5461 ccggccagtt cgatgttgtg aatattgccg ctgacacgat ctcgatattc cagaccaacg
     5521 actttgctgc cgtcgccttt cacttccgtg gtttgcgcat tcagaataat gtcgacgttt
     5581 ttcaggctgc gcagtttgtc ctgcagaacc tggtcggctt tcatttctgg cgcaaattcc
     5641 agcagcgtta cgtgctcaac gatacccgcc aggtcgattg ccgcttccac gccggagtta
     5701 ccgccgccga taaccgctac gcgtttgcct ttaaacagcg ggccgtcgca gtgcgggcag
     5761 taggtcacgc ctttagtgcg atactgatct tcgcccggaa cgttcatgtt gcgccatttt
     5821 gcacccgtcg ccacgataat gctgcgtgct ttcagtaccg cgccagaagc tgtttcaatc
     5881 tggtgtagac caccttcaac cgctgccggg atcagtttgc tggcgctctg gctgtcgatc
     5941 acatcaacgt cgtattcatc aacgtgaact ttcagtgcgc ctgccaactt ctgcccttca
     6001 gtcttcggta cagaaatgta gttttcgata tcaacggtat cgaggatctg accaccaaaa
     6061 cgttcgccca tcagaccggt acggatgcct ttacgtgcgg agtaaattgc tgccgctgca
     6121 cccgccgggc cggaaccaac gattaatacg tcataagcat cacgcttgtt cagctcttct
     6181 gccgcacgat tttccgcgcc agtatcaatt ttggcaacga tttcagtcaa cgtcatgcgg
     6241 ccctgaccaa actctttccc gtttacgaac actgccggaa cgcccatcac gttgcgatcg
     6301 gtgatttcgt tctggaaggt gccgccgtca attgcagtgt gcttgatgcg cgggttcaat
     6361 acgctcatca ggttcagcgc ctgcaccacg tccgggcagt tatggcaaga gagcgagtaa
     6421 taggtttcga attcaaaatc accgtcaata tggcgaatct gctccagcag agactgcgct
     6481 tctttcgacg gatgaccacc ggtccacagc aacgccagta ccagcgaggt gaactcgtgg
     6541 cccagcgggg agcctgcaaa acgtggtccc tggttggaac ctgggttggt gatcaggaaa
     6601 gacggcttac gcactggcaa gctgttatct tctttaaagg tgactttgtc tgacagttct
     6661 gcgatttcag ccaacagttc cttgatttct gccgatttag cgctgtcatc cagcgtggca
     6721 attaactcaa caggcttggt caatttctca aggtaagcct tgagttgagt tttcatattt
     6781 gtgtcgagca tgtttatctc ctgggcttta aacatcatca tgcaagcggc ctctgcggct
     6841 acctggatgc agcttgcatc atggtgcggg gaagaaaatc gggtgccgca gcacccgaag
     6901 aattaggtga atttccgaag tatttcgcgt cagagcaagg cgactagcct gtgaatcccc
     6961 gggagcttac acaagtaagt gaccggggtg agcgggcgac gccaacgccg ctatggcgtg
     7021 aaagacgaag gaaatttaga ttttaccaac caggtccaga gacggagcca gagttgcttc
     7081 accttctttc catttagccg ggcaaacttc acctgggtga gaagctacgt actgagctgc
     7141 tttgatttta cgcagcagat cagacgcgtc acggccaatg ccttcagcgg taacttcgat
     7201 tgcctggatg ataccctgcg ggtcaacaac gaaggtcgca cggtcagcca gaccttcatc
     7261 ttcacgcatg ttgtcgaagt tacgggtcag ggcgccagtc gggtcgccga tcatcgcata
     7321 tttgatttta gcgatggttt cagagctgct gtgccatgct ttgtgggtga agtgagtatc
     7381 ggtagatact gcgtatacgt ctacgcccag tttctgcagt tcttcgtagt ggtcagcaac
     7441 gtcacccagt tcggtcgggc atacgaaagt aaagtcagcc gggtagaaga agaagacgct
     7501 ccagcggcct tcggtatctt tttcggtgat ttcgatgaat tcgccgtttt tgaatgcctg
     7561 gtttttaaaa ggtttaattt tggtgttaat caaggacatc tatacttcct ccgtgttttc
     7621 gatgagatgt aaggtaacca atttttgccg attcggctaa tgcgtttcca ttatcaaatc
     7681 gataagtttt accttacaac cttcgtaaga caactttgtg aactttgcac cgccagcggc
     7741 gataaaaagt aaaatggccc cctttccagg tgaccgtgta atctgaacta ccctttggta
     7801 tcttcagcgc ctgcctgcag gctgaggtgt tgcccttaca aatgcaacaa cgacatggat
     7861 tacaacaccc tcataaacaa agggcaatca cctgatctaa gctcttacct atgacagtga
     7921 taggttatgc cttttactcg acttttgcac tgactgaaaa gaacaaatta atgttaaaaa
     7981 agatactttt actggctctg cttcctgcaa tcgccttcgc agaggaactt ccttccccag
     8041 taaaagcgat tgaaaaacag ggcattacaa tcatcaaaac gttcgatgcc cccggaggaa
     8101 tgaaaggtta tctcggaaag tatcaggata tgggcgtcac catctacctg actcccgatg
     8161 gtaagcacgc tatctctggt tacatgtaca acgagaaagg tgaaaacctg agtaacacac
     8221 ttatcgaaaa agaaatttac gcaccagccg gacgcgaaat gtggcaacgg atggaacaat
     8281 cccactggct cctcgacggt aaaaaagatg cgccggtcat tgtctacgtc ttctccgatc
     8341 cgttctgccc atattgtaaa cagttctggc agcaggcgcg cccgtgggta gattctggca
     8401 aagtgcaatt aagaacattg ttggttgggg ttatcaagcc agaaagcccg gcgacagcag
     8461 cggcaattct tgcctccaaa gatcccgcaa aaacctggca acaatatgaa gcctctggtt
     8521 gcaagcttaa gctaaacgtg cctgcaaacg taagtacaga gcaaatgaaa gtgttaagtg
     8581 acaatgagaa actgatggac gatctggggg caaatgtcac gccggctatc tattacatga
     8641 gtaaggaaaa tacgctgcaa caggccgtgg ggttgcccga tcagaaaacg cttaatatca
     8701 ttatgggaaa taaataaaag gtaattcatg gatggttgaa ctatatccct gcttttcaac
     8761 catcctaaaa aagcatgaat ttctcgctct accctaagtg gctgtgctat tttagaaaaa
     8821 cttaatcctg agttaagcaa aaaaacataa tcaataaaaa tacatacgca aaaaacattg
     8881 attaagtgaa tataccatgg aagaaaaata taaaccggag tagtgtatgg ccaatctcta
     8941 cgacttgaaa aagttcgact taaatcttct tgtcatattt gagtgtattt accagcattt
     9001 aagcattagc aaagctgctg aatcactata tatcacccca tcagccgtga gccagtcgtt
     9061 acaacgctta cgtgcgcaat ttaatgaccc tttattcatt cgctcaggaa aaggtatcgc
     9121 gccgacgaca acagggttga atctgcatca ccatcttgag aaaaacctca aaggccttga
     9181 acaaacaatt aatatagtca ataagtccga actaaagaaa aactttatta tctacggccc
     9241 tcagctcatt tcttgctcta ataatagtat gctgatccgc tgcctacggc aagatgcctc
     9301 tgtcgagatt gagtgccatg atatcctcat gtcagcagag aatgctgaag aactgttggt
     9361 tcaccgcaaa gccgatctgg tgatcaccca aatgccagtg atcagccgtt ccgtcatttg
     9421 catgccattg catacaattc gcaatactct aatttgtagt aataagcatc cgcgaattac
     9481 tgacaatagc acttatgaac aaattatggc agaagagttc acccaattaa tagccaaatc
     9541 agcaggagtt gatgatattc aaatggaaat agacgagaag tttatgaatc gcaaaatttc
     9601 atttcgtggt tcctctttgc tgacaataat taatagcatt gcagttacag atttactggg
     9661 aatagtcccc tatgaacttt ataactacca ccgtgacttt cttaatttaa aagagataaa
     9721 actagaaca
//
LOCUS       DAZPAB010000144         9706 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZPAB010000144 DAZPAB010000000
VERSION     DAZPAB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9706
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.144"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(62..802)
                     /gene="cysQ"
                     /locus_tag="ACHUPG_003043"
     CDS             complement(62..802)
                     /gene="cysQ"
                     /locus_tag="ACHUPG_003043"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR0862563.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            992..2935
                     /gene="cpdB"
                     /locus_tag="ACHUPG_003044"
     CDS             992..2935
                     /gene="cpdB"
                     /locus_tag="ACHUPG_003044"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR0862564.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(3061..3441)
                     /locus_tag="ACHUPG_003045"
     CDS             complement(3061..3441)
                     /locus_tag="ACHUPG_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR0862565.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            3530..4390
                     /gene="qorB"
                     /locus_tag="ACHUPG_003046"
     CDS             3530..4390
                     /gene="qorB"
                     /locus_tag="ACHUPG_003046"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR0862566.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            4499..5464
                     /locus_tag="ACHUPG_003047"
     CDS             4499..5464
                     /locus_tag="ACHUPG_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0862567.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            5572..6234
                     /gene="ytfE"
                     /locus_tag="ACHUPG_003048"
     CDS             5572..6234
                     /gene="ytfE"
                     /locus_tag="ACHUPG_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR0862568.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6279..7691)
                     /gene="cycA"
                     /locus_tag="ACHUPG_003049"
                     /gene_synonym="dagA"
     CDS             complement(6279..7691)
                     /gene="cycA"
                     /locus_tag="ACHUPG_003049"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR0862569.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(7999..8619)
                     /gene="fklB"
                     /locus_tag="ACHUPG_003050"
     CDS             complement(7999..8619)
                     /gene="fklB"
                     /locus_tag="ACHUPG_003050"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0862570.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            8838..9383
                     /locus_tag="ACHUPG_003051"
     CDS             8838..9383
                     /locus_tag="ACHUPG_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HFR0862571.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(9367..>9706)
                     /locus_tag="ACHUPG_003052"
     CDS             complement(9367..>9706)
                     /locus_tag="ACHUPG_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR0862572.1"
                     /translation="FLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYN
                     IFKPGIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWL
                     LVDPSSTAHE"
BASE COUNT         2476 a         2532 c         2460 g         2238 t
ORIGIN      
        1 tttaggttgg gataaatcag gatggcagaa tcaggaaata cactgtttct gccatctgaa
       61 tttagtaaat ggacactctg aaccccggat tcaggaacga ctcgcgcgga gtgtaatcca
      121 gcggtttacc ctgccagtcg tgaacgtgcg ctccggcagc tgcagccaca gcatgtccgg
      181 cggcggtgtc ccaaatattc gttggtccga agcgcgggta cagctgcgcc tgtccttccg
      241 ccaccaggca gaatttcagc gaagagccga tggacgtggt ctgatgttcg ccaagctgtt
      301 gcagatactc tttcagctcc gcatctgcat gggaacggct gataaccacc agcggcgggc
      361 gcgcatcgcg gacctgaatc agcttgcgca caccgcactc ttctttccag gccttgcctt
      421 ctgccgcgct gtacattacg ttcattaccg gcgcatacac cacacctaaa atcggtttgc
      481 catggtcaat gagcgcaatg ttaacggtga attcgccatt acgtttaata aactctttag
      541 taccatccag cgggtctacc agccagtaac gctgccagtg ctgacggact tcccaaccgg
      601 gaggatcttc ttcagaaagg accggaatat ccggtgccag cgtacgtaaa ccgtccatga
      661 taacggtgtg agcggcaata tccgctgccg ttaccggaga attgtccgct ttgctgacga
      721 cgtccatcgg tttcgtcccg tcgtagacct gcataatggc atcgcctgca ttccgtgcaa
      781 gctggcatac ttgatctaac atttctccac ctcgtctctg tgagcggtgt taacttattg
      841 ttttacttat accctatcgt taatgaatgc gccaactgtg atagtgtcat cattttcaaa
      901 gcgtaaaatt gtggcattct tcactgttct ataagtaagg cgtttattct cccttttctt
      961 tcgtattccc gatgataaaa ggatgtccct gatgattaag tttagcgcaa cgctcctggc
     1021 cacgctgatt gccgccagtg tgaatgcagc gacggtcgat ctgcgtatca tggaaaccac
     1081 tgatctgcat agcaacatga tggatttcga ttattacaaa gacaccgcca cggaaaaatt
     1141 cggactggta cgtacggcaa gcctgattaa cgatgcccgc aatgaagtga aaaacagcgt
     1201 actggtcgat aacggcgatt tgattcaggg gagtccgctg gccgattaca tgtcggcgaa
     1261 aggattaaaa gcaggtgatg ttcatccggt ttataaggcg ctgaatacgc tggattatac
     1321 ggtcggtaca ctcggcaatc atgaatttaa ctacggtctg gattacctga aaaatgcgct
     1381 ggcgggagcg aaattccctt atgtaaatgc caacgtcatt gacgccagaa ccaaacagcc
     1441 aatgtttaca ccgtatttaa ttaaagatac cgaagtggtc gataaagacg gaaaaaaaca
     1501 gacgctgaag attggctata ttggcgtcgt gccgccgcaa atcatgggct gggataaagc
     1561 taatttatcc ggaaaagtga cggtgaatga tattaccgaa accgtgcgca aatacgtgcc
     1621 tgaaatgcgc gagaaaggtg ccgatgtcat tgtcgttctg gcgcattccg ggctgtctgc
     1681 cgatccgtat aaagtaatgg cggaaaactc agtttattac ctcagtgaaa ttccgggcgt
     1741 taacgccatt atgtttggcc atgctcacgc cgttttcccg ggtaaagatt ttgctgatat
     1801 cgaaggggct gatatcacca aaggcacgct gaatggtgtt ccggcggtaa tgccgggcat
     1861 gtggggcgat catcttgggg tggtcgactt acaactcagt aatgacagcg gtaaatggca
     1921 ggtgacgcag gcgaaagcgg aagcacggcc gatttacgac atcgccaata aaaaatccct
     1981 cgcggcggaa gacagcaagc tggtagaaac actcaaagcc gatcacgatg ccacacgcca
     2041 gttcgtcagc aagccaatcg gtaaatctgc cgacaatatg tatagctatc tggcgctggt
     2101 gcaggacgat ccgaccgtgc aagtcgtgaa caacgcgcaa aaagcgtatg tcgaacatta
     2161 cattcagggc gatccggatc tggcaaaact gccggtgctt tcagctgccg caccgtttaa
     2221 agttggtggt cgcaaaaatg acccggcaag ctatgtggag gtggaaaaag gccagttgac
     2281 cttccgtaat gccgccgatc tttatctcta ccccaatacg ctgattgtag tgaaagccag
     2341 cggtaaagag gtgaaagagt ggctggagtg ctccgccgga cagtttaacc agattgctcc
     2401 taacagcacg aaaccacagt cactcatcaa ctgggatggt ttccgcactt ataactttga
     2461 tgttattgat ggtgtgaatt atcagattga tgttacccaa cccgcccgct atgacggcga
     2521 gtgccagatg attaatgcca atgcagagcg gattaagaac ctgaccttta atggcaagcc
     2581 gattgatccg aacgctatgt tcctggttgc caccaataac tatcgcgctt acggcggcaa
     2641 atttgccggt acgggcgaca gccatatcgc ttttgcttca ccggatgaga accgctcggt
     2701 gctggcagcg tggattgctg atgagtcgaa acgtgcgggg gaaattcatc cggcggcaga
     2761 taacaactgg cgtttagcac cgatagctgc cgataagaaa ctggatatcc gtttcgaaac
     2821 ttccccgtca gataaagccg cagcgtttat taaagagaaa gggcagtatc cgatgaataa
     2881 agtcgcgacc gatgatatcg ggtttgcgat ttatcaggtg gatttgagta agtaaaacac
     2941 ttctttttcg gcctataaat caaccgcatc cggcatttat tggcggatgc gatgctgccg
     3001 tatcttatcc accctacaag ccatgaaccg tagaccggat aagcgcagcg catccggcag
     3061 ttatgccgca cgttcatccc gcaccgccaa cacctcgggc aaattcaact caatccagtc
     3121 cgccagtgca gcaacctttt cgctcacctg ctcgcccagc ggcgtgaggc tatattccac
     3181 atgcggcggc accaccggat acgcgatacg gttaagaaaa ccatcctgtt ctaacgcctg
     3241 taacgactgc gcaagcatct tttcactcac cccaccgatt ttgcgccgca ggtcgctaaa
     3301 gcgatgagta ccttcgcgta gcgccaccag aatcaacacc ccccaacggc tggtgacgtg
     3361 tttcaacacc tcgcgcgacg ggcactgttc cgcaaagaga ttaccctctt tcagttgttg
     3421 tgacaggcta acctgactca tttcatactt acctttttgc acgtacttac taaaagtaag
     3481 tttaggtgtt agcgtattta aacacaagac aaaacgatgg agacttccca tgatcgctat
     3541 tactggtgcc actggccaac ttggtcacta tattattgaa tccttgatga aaacggttcc
     3601 tgccagccaa atagtggcta tcgttcgtaa tccggcaaaa gcccaggccc tggcagcaca
     3661 aggcattacc gtgcgtcagg ctgactacag cgatgaagcc gcactgacat ctgcacttca
     3721 gggagtggaa aaactactgc tgatctcttc cagcgaagtg ggtcaacgtg ccccgcagca
     3781 tcgtaatgtt attaatgccg caaaggcggc tggcgtgaaa tttatcgctt ataccagcct
     3841 gctacatgca gatacctccc cgctcggcct cgccgatgag cacatcgaga cggagaaaat
     3901 gctggctgat tctggcatcg tttacaccct gctgcgcaac ggctggtaca gcgaaaacta
     3961 cctcgccagc gccccggcag cactggaaca cggcgtattt atcggtgcgg ctggcgatgg
     4021 caaaatcgcc tcagcaacgc gggcagatta tgcgacagct gcggcacgcg tgattagcga
     4081 agccggtcac gaaggcaagg tttacgaact ggcgggcgat agtgcctgga cgttgacaca
     4141 gttagcggca gagctgacca aacagagcgg caaacaggtt acctatcaaa atctgagcga
     4201 agccgatttc gccgcggcac tgaaaagcgt cggactgccc gacggactgg cggatatgct
     4261 ggcggattct gacgttggcg catcgaaagg tggtctgttt gataacagca aaacgcttag
     4321 caaattgatt ggctgcccaa cgacaacgtt agccgaaagc gtaagccatc tttttaatgt
     4381 taataactag ttaattaaag tggcatcctc ccgcatcctc tctgataatg acgggatgcc
     4441 gggagcaatc atgtctgctt cctgaacgtt tcttctgaca gaccaatgga tgccagtaat
     4501 gattagcggc gtgctgtacg ccctgttagc agggttgatg tgggggctta tttttgtcgg
     4561 gccgttgatc gtgccggaat acccggcgat gttgcagtcg atggggcgtt atctggcgtt
     4621 agggttaatt gcgctaccca ttgcctggct gggacgcgtg cgtctgcgtc agttggcgcg
     4681 tcgggactgg cttaccgcct tgatgctcac tatgatgggc aacctcattt attacttctg
     4741 ccttgccagt gccattcaac gtactggcgc gcctgtttcc acgatgatta tcggcaccct
     4801 gccggtggtg attcctgtct ttgccaatct gctttatagc cagcgcgacg gcaaactcgc
     4861 gtggggaaaa ctcgccccgg cactgatttg tattggcatc ggcctggcga gtgtgaatat
     4921 tgctgagtta aaccacggac tccccgattt tgactgggca cgttatacct ctggcatcgt
     4981 gctagcgtta gtttccgtgg tctgctgggc atggtatgcc ctgcgcaacg cccgctggct
     5041 gcgggaaaat cccgacaaac atccgatgat gtgggcgacg gcgcaggcgc tggtcacgct
     5101 gccggtttct ctcatcggct atctcgtcgc ctgttactgg ctgaatatac aaacgccgga
     5161 cttctcctta ccttttggcc cccgtccgct ggtgtttatt agtctgatgg ttgcgatagc
     5221 cgtgctttgc tcatgggttg gcgcactctg ctggaacgtc gccagccagc gattaccgac
     5281 agtgattctc gggccgctga ttgttttcga aacgctggca ggtttgctgt acaccttttt
     5341 actccgccag caaatgccgc cgctaatgac gctgagcggt atcgcgctgt tagtgattgg
     5401 cgtggtcatc gcggtcagag caaaaccaga aaaaccttta actgaatctg tctcagaaag
     5461 ttgacatgct ggcagtgagt taaataagcc tctgctacgt aagggttata gcttttgcct
     5521 taaaggtgta tttaaaatac atcttatctt attaagaatg aggtatcagc tatggcttat
     5581 cgcgaccaac ctttaggtga actggcgctc tctatccctc gcgcttcggc tctgtttcgt
     5641 aaatatgata tggattactg ctgtggcggt aagcagacgc tggcgcgcgc ggcggcacgt
     5701 aaagaactgg atgttgaggt cattgaagct gaactggcaa agcttgctga acaaccgatt
     5761 gagaaagact ggcgtagcgc cccgctggca gaaatcattg accatatcat cgtgcgctac
     5821 cacgatcgtc accgcgagca gctgccggag ctgatcctgc aagcgactaa agtcgagcgc
     5881 gttcacgccg acaaaccgag cgtgccaaaa gggctgacaa aatacctgac catgctgcat
     5941 gaagagcttt ccagccacat gatgaaagaa gagcagatcc tcttcccgat gatcaaacaa
     6001 ggcatgggca gccaggcaat ggggccaatc agcgtaatgg aaagcgagca cgatgaagcg
     6061 ggcgaactgc tggaagtgat caaacacact accaataacg tcacaccgcc gccagaagcc
     6121 tgcaccacct ggaaagcgat gtataacggc attaatgaac tgattgatga cctgatggat
     6181 cacatcagtc tggaaaacaa tgtactgttc ccacgcgccc tagcgggtga gtgatctaaa
     6241 agctggatgg cattgcgcca tccagcatga taatgcggtt atttccgcag ttcagcagcc
     6301 cgcttcttac caataaacag ccagcccaac cccagcgcga taaaccacag cggggtaacc
     6361 agcagcgcct ggcgagtgtc atcttccagt gtcagcaaca cgaccacgaa cacaaagaac
     6421 gccatacata cccagcacat cagcttgccg agcggcatct tgtagatcga cttttcatgc
     6481 agatgaggac gctgtttgcg gtacaccagg taagagcaaa ggataatcgt ccagacgaac
     6541 ataaacagaa tcgcggaaac ggttgtaatc atcgtgaacg cgccaatcac gctaggattc
     6601 acatacaaca tcaccacgcc gccgagcaga cagatacacg agaaggtcaa ccctttcgcg
     6661 ggtactgcgc gcttagaaag tttagcgaac gctttcggcg caacaccttc ctgcgccaga
     6721 ccaaacagca tacggctggt agagaagaca ccgctgttag cggaagacgc cgcagaggtc
     6781 agcaccacaa agttgatcac gctggcagcc gcaggcaaac ctaccaacac aaacagctca
     6841 acgaacgggc ttttctccgg gactaccgaa ctccacggtg tcacggacat aatcacaatc
     6901 agcgcgaaga cgtagaacat aatgatacgg atcggaatgg agttaatcgc gcgtggcagt
     6961 gatttctccg gatctttggt ttccgcagct gttgtaccta ccagctcaat ccccacgaaa
     7021 gcgaaaaccg ctatctggaa tccggcaaag aagccactta aacctttcgg gaaccagccg
     7081 ccgtcattcc acaaatgcgc gaatgacgct tccacaccgg tcggtgactg aaagtgcatc
     7141 gccaccatga ccaggccgac gacaatcagc gacacgatgg cgacgatttt gatcatcgca
     7201 aaccagaact ccatctcacc gaacattttc acggtggcaa ggttgagcgt cagcagcagc
     7261 actatcaccg ccagcgaggc gacccagtcg gagagatcgg ggaaccagaa ctgagcataa
     7321 gccgtgatcg ccaccacgtc tgccataccg gttacaaccc agcagaacca gtaagtccag
     7381 ccggtgaaat atcccgccca cggccccagt aaatcggaag cgaagtcact aaaagatttg
     7441 tattccagat tcgaaagcag caattccccc attgcccgca tcacgaaaaa gagcataaaa
     7501 ccaatgatca tataaacgaa aatgatcgac ggcccggcaa ggctaatcgt tttgccggac
     7561 cccataaaca acccagtacc aatggcacct ccaatggcaa taagctgaac atgtcggttt
     7621 gtgagattgc gccgtagcga ctgttcagcc ggagcctgat catcggcaac gacttttacc
     7681 tgatctacca tgttttttct tcctgtacct gtctgtgttg ttcaggctct acggccttta
     7741 gtcagtctat gatacgacga atctgtatcg ggactcatcg atattaggta acaatacgcg
     7801 ggatgaatac taagatttag atctaatgtt aattttatgt ttaaagtgag tgtcatatca
     7861 cggtgataac gcgaaacagc tcacaaaaat acacgcttac ggggtctttg caacacaaaa
     7921 tagcgccgtt tggcaaactg aagggtttat tgctgaatgc ctgctcccct ctggttcgag
     7981 gggaacagaa tgcgctgctt agaggatttc cagcagttcg acttcaaaca ccagggtgct
     8041 gaacggaggg atggatgcac ctgcgccgcg ctcgccatat gccagttcct gcgggatagt
     8101 cagttcccat ttagaaccta ccggcatcag agtcagtgct tcaatccagc cagggatcac
     8161 gccattaacc gggaattcag cgggttcacc acgagcaacg gagctgtcaa acacggtgcc
     8221 gtcgatcagt ttaccggtgt aatgaacacg aacgcggtcg gtacgtgccg gaattgcgcc
     8281 ttcaccctgg ttgatcacgc ggaattgcag gccagattcg gtgctattca caccttcttt
     8341 tttggcgttt tcttccaggt atttcacgcc ttcagcagcc atcgcctgga aacgctgacg
     8401 acgaacggca tcggcgcgtt cgtggatttc acgcagcgcg cgatgcacca catcaaccgg
     8461 cacagccgga tgtttgcctt ccagcgcatc ggcaatacct gcaaccagtg cttctggcag
     8521 cagcccttcc aggccagatt cactcagttg ttgccctacc tgcaaaccaa tgccgtagct
     8581 tgcttgcgct tcgatggtgt caaaagttgg ggtggtcatc gtatttcctt tcttagggta
     8641 taaaagtagc gggcagcata tcagccacac ttctttgggt aaaactttgt cgcaggaatg
     8701 atgacaaatc ccggggaaaa agaaacaata gcaatatccc gtatgaatat aataaaggcg
     8761 ggtttaccac ggacatcata gcgttagctt attcataggc tatgattgag gaacaagacg
     8821 tggagcagga ggaaaccatg cccgggcgct ttgaactaaa accaaccctg gagaaagtct
     8881 ggcacgcgcc ggacaatttt cgcttcatgg acccgctgcc gccgatgcat cgtcggggga
     8941 tcattattgc cgccattgta ttggtggtcg gatttctgct tccatctgat gatacgccca
     9001 acgcgtcggt ggtgacgcgt gaagcgcagt tagatattca gtcgcaatca caaccgccga
     9061 cagaagaaca gccgcaaact acccaaacgc aaccattcca gccagatagc ggaattgata
     9121 atcaatggcg ctcttaccgt gtcgaacccg gtaaaacgat ggcgcagctg ttccgcgacc
     9181 acggactgcc cgctaccgat gtctatgcaa tggcgcaggt agaaggcgcg ggtaagccgc
     9241 tgagtaattt gcaaaatggg cagatggtga aaatccgcca gaacgccagc ggcgtggtga
     9301 cgggcttaac cattgatacg gggaataatc aacaggtcct gtttacccgc cagccggatg
     9361 gcagttttat tcgtgcgcgg tagatgaagg gtcaaccaaa agccagctaa ggattgtctc
     9421 tttgatgacc ttacgtctgg cttccagtgc ctggggggaa accatactga tatgataaac
     9481 taggccaagc gtatgctggt tgatcaaata atatccccca agcgcagcaa tattaatatt
     9541 cacctgcaat gggtcgatac ctggcttaaa aatattgtat ttcttccctt catccaataa
     9601 ggattccatt aactgcaagt gagcatggtt aatttctaag agacgctgtg attttgcata
     9661 atgcacacct ttactttgat tttcactatg cacaattttt aaaaac
//
LOCUS       DAZPAB010000145         9680 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZPAB010000145 DAZPAB010000000
VERSION     DAZPAB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.145"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(44..214)
                     /locus_tag="ACHUPG_003053"
                     /pseudo
     CDS             complement(44..214)
                     /locus_tag="ACHUPG_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACHUPG_003054"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="ACHUPG_003054"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR0862573.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="ACHUPG_003055"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="ACHUPG_003055"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR0862574.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /gene="frdB"
                     /locus_tag="ACHUPG_003056"
     CDS             3536..4270
                     /gene="frdB"
                     /locus_tag="ACHUPG_003056"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR0862575.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="ACHUPG_003057"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="ACHUPG_003057"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR0862576.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="ACHUPG_003058"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="ACHUPG_003058"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR0862577.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5109..6241
                     /locus_tag="ACHUPG_003059"
                     /pseudo
     CDS             5109..6241
                     /locus_tag="ACHUPG_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            6330..6863
                     /gene="blc"
                     /locus_tag="ACHUPG_003060"
     CDS             6330..6863
                     /gene="blc"
                     /locus_tag="ACHUPG_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR0862578.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6860..7177)
                     /gene="sugE"
                     /locus_tag="ACHUPG_003061"
     CDS             complement(6860..7177)
                     /gene="sugE"
                     /locus_tag="ACHUPG_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR0862579.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7353..7499)
                     /gene="ecnB"
                     /locus_tag="ACHUPG_003062"
     CDS             complement(7353..7499)
                     /gene="ecnB"
                     /locus_tag="ACHUPG_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR0862580.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7610..7735)
                     /gene="ecnA"
                     /locus_tag="ACHUPG_003063"
     CDS             complement(7610..7735)
                     /gene="ecnA"
                     /locus_tag="ACHUPG_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR0862581.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7787..8353)
                     /gene="efp"
                     /locus_tag="ACHUPG_003064"
     CDS             complement(7787..8353)
                     /gene="efp"
                     /locus_tag="ACHUPG_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR0862582.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8395..9423
                     /gene="epmB"
                     /locus_tag="ACHUPG_003065"
     CDS             8395..9423
                     /gene="epmB"
                     /locus_tag="ACHUPG_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR0862583.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2465 a         2479 c         2466 g         2270 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac
     5101 cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat
     5161 ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
     5221 aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta
     5281 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
     5341 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
     5401 aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga
     5461 atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc
     5521 cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat
     5581 gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg
     5641 ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac
     5701 tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg
     5761 gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg
     5821 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta ccccccttga
     5881 tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct actggcaaac
     5941 cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca atcctgacag
     6001 catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa aagcgattaa
     6061 gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga ccggcggatt
     6121 tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc tggctaacaa
     6181 aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg ctctacagta
     6241 aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct gtcatctaca
     6301 cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt gccgcagcga
     6361 cagctgcatt tctggtcgtt gcctgtagtt ttcctacgcc gccgcgtggc gtgaccgtag
     6421 tgaataattt cgacgccaaa cgttatctcg gtacctggta tgagattgcc cgttttgatc
     6481 accgttttga acgtggactg gaaaaagtca ccgcaacata cagcctgcgt gatgacggcg
     6541 gcctgaatgt cattaataaa ggctataacc ctgacagagg aatgtggcag cagagtgaag
     6601 ggaaagcgta ctttaccggc gcaccaactc gcgctgcgct gaaagtgtca ttctttggtc
     6661 ctttctatgg cggttataac gttattgcac tcgatcggga ataccgccat gcgctggttt
     6721 gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt tctgacgaag
     6781 tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt aagtttattt
     6841 gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc cccaatacga
     6901 ttaacgccag actcgccaga cgcatcgggt tagcggactc accgagcagc acaatgccgg
     6961 tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc cctaccggta
     7021 acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc acggtaataa
     7081 cactcggcgt caaacggcta aagccgtggg tatatttcag gccaacggcc cataccactt
     7141 ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga aatttggggc
     7201 cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt tgaacggata
     7261 ttttgccagg tggcagggga atttcaacta ttacctttat gacgatcgca gaaaaaacaa
     7321 acggcacgac actgctgtcg taccgttatt gcttattgct gcgctttcgt tgctgcgcca
     7381 gaaatcgcgt taccgccatc agaaatgtct tcaccaacgc cacgcgtggt gttgcaggca
     7441 gttaatactg ttgaaagcac cagaacagaa aagatcgctg caattgtctt cttcaccata
     7501 acgtcttcct tttaggtaat gttatttatg tttgcctata gcaaattaat aatagacaac
     7561 atcgccaaaa atgatggaaa tctgatgatt tttcggaaga gaagaaaaat tagctggcag
     7621 cgcgagagat ggagttaccg agatgtttga tgtcttcgcc gaaaccgcaa gcggtgttac
     7681 agcccgtgag cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc atcatctctg
     7741 acctgaataa gtgatggtgc agcctgcagg ccgcaccaca accgcattac ttcacgcgag
     7801 agacgtattc accagagcgg gtatccactt tgatgacttc gccgatttgt acgaacagcg
     7861 gaactttaac cacagcgcca gtagacaggg tagccggttt gccgccagta cctgcggtat
     7921 cacctttcag gcccggatcg gtatcaacga tttccagttc aacgaagttc ggcggagtaa
     7981 cggagatcgg ctgaccattc cacagagtta cgatacactc tgcctgatcc agcagccatt
     8041 tagcattgtc gccaattgct tttgcatcag cagacagctg ctcgaaagtt tcgttgttca
     8101 tgaagtgcca gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg acaacatcag
     8161 cgccttcagc ggaatcagta gatttgaagg ttttttctac gcgagtaccg gtcagcagac
     8221 gacgcagttt aacgcgagca aatgcctggc ctttacccgg ttttacgaat tcactcgctt
     8281 caaccgcgta aggttcgccg tctaacatga ttttaagacc agcacgaaaa tcgttgctat
     8341 agtacgttgc cataaggccc tctgaaattt gttaattggt agctaagcca caaaatggcg
     8401 catattgtaa ccctaaatac cccatccaga gaagattggt taacgcaact tgccgatgtt
     8461 gtgaccgatc ctgatgaact tctgcgtctt ttgaatatag acgcggacga aaaactgttg
     8521 gccggacgca gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt tatcgatcgc
     8581 atggagaaag gcaatccgga cgatcctctt ttgcgtcagg tacttacctc gcaagatgag
     8641 tttgtcgtcg cgtccggatt ctccaccgac ccactggaag aacagcacag cgtagtgcct
     8701 ggtttgttgc ataaatacca caaccgggcg cttttgctgg tcaaaggcgg ctgcgcggta
     8761 aattgccgct attgcttccg tcgccacttc ccctatgccg aaaatcaggg caacaagcgt
     8821 aactggcaga ctgcacttga gtatgttgct gcgcatccgg aactggacga gatgattttc
     8881 tccggcggcg atccgctgat ggcgaaagag cacgagctgg actggttgct cacacaactg
     8941 gaagccatcc cgcatatcaa acgtctgcgg attcacagcc gtctgccgat tgtgatcccg
     9001 gcacgtatca ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca aatcttgctg
     9061 gtgaatcaca tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc gatggctaaa
     9121 ttgcgccgtg tcggtgtcac cctgcttaac cagagcgttc tgttacgtgg tgtgaacgat
     9181 aacgcacaaa cgttggcaaa cctgagtaat gcgttgttcg atgccggcgt gatgccgtat
     9241 tacctgcatg tgctagataa agtacagggc gcggcgcatt ttatggtgag tgatgacgaa
     9301 gcacggcaga ttatgcgtga gttgctgaca ttggtgtcgg gatatctggt gccgaaactg
     9361 gcgcgagaaa ttggcggcga acccagcaaa acgccgctgg atctccagct acgccagcag
     9421 taactgatta aacagggaag ttaaacatta aaattgcctg atgcgctacg cttatcaggc
     9481 ctacatcgtg cattgaaatt gaatggattt gcaatgaact gtaggccgga taaggcgttt
     9541 acgccgcatc cggcaagagc aacaaacgca agtacagcaa ccactgagaa atttaatctt
     9601 tgcacgttgc ataaccttta attattcata tgcattgctg ttgttacttt ttatatagct
     9661 aacaccaata attaaccttt
//
LOCUS       DAZPAB010000146         9593 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZPAB010000146 DAZPAB010000000
VERSION     DAZPAB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9593
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.146"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            11..1735
                     /gene="iucA"
                     /locus_tag="ACHUPG_003066"
     CDS             11..1735
                     /gene="iucA"
                     /locus_tag="ACHUPG_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR0862584.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            1736..2683
                     /gene="iucB"
                     /locus_tag="ACHUPG_003067"
     CDS             1736..2683
                     /gene="iucB"
                     /locus_tag="ACHUPG_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR0862585.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            2683..4425
                     /gene="iucC"
                     /locus_tag="ACHUPG_003068"
     CDS             2683..4425
                     /gene="iucC"
                     /locus_tag="ACHUPG_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR0862586.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            4422..5759
                     /gene="iucD"
                     /locus_tag="ACHUPG_003069"
     CDS             4422..5759
                     /gene="iucD"
                     /locus_tag="ACHUPG_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR0862587.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            5765..7960
                     /gene="iutA"
                     /locus_tag="ACHUPG_003070"
     CDS             5765..7960
                     /gene="iutA"
                     /locus_tag="ACHUPG_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR0862588.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(8511..8654)
                     /locus_tag="ACHUPG_003071"
     CDS             complement(8511..8654)
                     /locus_tag="ACHUPG_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862589.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            8893..>9593
                     /locus_tag="ACHUPG_003072"
     CDS             8893..>9593
                     /locus_tag="ACHUPG_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR0862590.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQW"
BASE COUNT         2365 a         2391 c         2493 g         2344 t
ORIGIN      
        1 ctgtttgact atgaccctgc cctctgaaaa accagccaca gatgtggctg cgcagtgctt
       61 cctgaatgca ctgattcgtg aaaccacaga ctggaaactg acagaatacc cgccagacga
      121 attgctcatc ccgctggatg agcagaaatc gctccatttc agagtggctt atttctcccc
      181 aacccagcat caccgctttg catttcctgc acgtctggtc acggcatcag gcagttatcc
      241 tgtcgacttt accactctct cccggcttat tattgataag ctacgccatc aactgtttct
      301 gcccgttccc ctctgcgaaa ctttccacca gcgcgtgctg gaaagccatg tccatacgca
      361 acaggcaatt gatgcccgtc atgactgggc cgctctgcgt gaaaaagcgt tgaattttgg
      421 cgaggctgag caggcgctgc tgacaggaca cgctttccac cctgcgccta agtctcatga
      481 accgtttaac cggcgggagg ctgaacgcta cctgcctgat atggcacccc acttcccact
      541 gcggtggttt tcggtggata aaacgcaaat cgccggtgaa agtttgcatc tcaaccttca
      601 acagcggctg acgcgatttg ccgcagagaa tgcgcctcag ttactcaacg aattaagtga
      661 caaccaatgg ctgttcccgt tgcacccgtg gcagggggaa tatcttttgc agcaggggtg
      721 gtgccaggca cttgttgcta aagggctgat taaagactta ggtgaggccg gcacgtcgtg
      781 gctgccgacc acctcttccc gttccctcta ctgtgccacc agccgcgata tgatcaagtt
      841 ctccctgagc gtacggctga ccaactccat ccgtactctg tccgtgaaag aagtgaagcg
      901 tgggatgcgc ctggcacgcc tggctcaaac cgacggctgg cagatgctac aggtccgctt
      961 cccgactttc cgggtaatgc aggaggatgg ctgggccggg ctgctcgatc ttaacggcaa
     1021 catcatgcag gaaagtctgt ttgccctgcg tgaaaatctg ctggtggatc agccgaaaag
     1081 ccagactaac gtactggtct ccctgactca ggccgcacct gatggcggtg attcgctgct
     1141 ggtttcggcg gtaaaacgcc tgagcgatcg cctcggtatc actgtgcaac aggccgccca
     1201 tgcatgggtt gatgcatact gtcagcaagt gctaaagccg ctgtttacgg ctgaagcgga
     1261 ttacggcctg gtgctgctgg cgcatcagca aaatattctt gtccagatgc ttggggatct
     1321 gccggtcgga tttatttacc gtgactgtca gggcagcgct tttatgcctc acgcgacaga
     1381 ctggctcgat tctattggcg aggcgcaggc ggaaaatatt ttcacccatg agcagttgct
     1441 gcgctatttc ccttattacc tgctggttaa ctccactttt gctgtgaccg ctgcgctggg
     1501 ggctgccggg ctggacagcg aatcgaatct gatggctcgt gtacgagcat cgctggctga
     1561 agtgcgtgat caggtgactc ataaaacctg tctcaactac gtgctggaaa gtccgtactg
     1621 gaacgtaaaa ggtaactttt tctgttatct gaacgatcat aacgagaaca ccattgttga
     1681 cccttcggtg atctatttcg atttcgctaa cccgctgcag gctcaggagg tctgaatgtc
     1741 tgaggcaaac attattcaca gcagatatgg actgcgctgt gaaaaactcg acaagcccct
     1801 gaatcttggc tggggactgg acaatagcgc ggtgttgcac tgtcccgggg agctgccgac
     1861 agggtggctg tgcgatgcgc tggatcagat atttatcgcc gcaccacaac tgtcagcagt
     1921 ggctctcccc tgggccgaat ggcgtgagga gccacaggcg ctgacgcttt ttgggcaggt
     1981 aaaaagcgac attatccatc gtactgcctt ctggcagtta ccgttatggt tgagttcacc
     2041 ggcaaaccgg gcctccggtg aaatggtttt tgatgcagag cgtgagattt atttcccgca
     2101 gcgcccaccc cgtccacagg gtgaggttta ccgccgttac gatccacgta ttcgcaggat
     2161 gctgagtttc cgcattgccg atcccgtttc tgatgcagaa cgtttcaccc gctggatgaa
     2221 tgatccgcgc gttgagtatt tctgggagca aagtggctca ctggaggtac agaccgccta
     2281 tctggagcgc cagctcaccg gtaaacatgc attcccgctg attgggtgct tcgacgatcg
     2341 gccattcagc tatttcgaaa tctactgggc ggcggaagac cgcattggtc gtcactattc
     2401 gtggcagccc tttgaccgtg gcctgcatct gctggttggt gaacagcaat ggcgcggggc
     2461 ccactatgtg caaagctggc tgcgcgggct gacacattac ctgctgctgg atgagccccg
     2521 tacgcagcgc acagtactgg agccacgcac cgataaccag cgcctgttcc gccatcttga
     2581 gcctgcggga taccggacaa ttaaagagtt cgacttccca cataagcgct cgcgcatggt
     2641 gatggcggat cgtcatcact tcttcacgga ggtcggtctg taatgaatca caaggactgg
     2701 gattttgtta accgtcggct ggtggcaaaa atgttgtctg agatggagta tgagcaggtt
     2761 ttccacgcgg aatctcaggg cgatgaccac tactgcatta acctgccggg agcacaatgg
     2821 cgcttcatcg ctgaacgtgg tatctggggc tggctgtgga ttgatgccca aacgttgcgc
     2881 tgcacggacg aaccagtact ggcgcagacg ctgctgatgc agctaaagcc ggtgctgtca
     2941 atgagtgatg ccactgttgc agagcatatg caggatttgt atgccacgct actgggcgac
     3001 ctgcaactgc tgaaggcccg tcgcggactg agtgccagtg acctgattaa tcttgatgcc
     3061 gatcgtctgc aatgcctgct gagcggtcat cctaaattcg tttttaataa aggtcgccgt
     3121 ggctggggca aagaggcgct ggaacgatat gcgccagagt ataccaacac cttcagactg
     3181 cactggctgg cggtaaaacg tgaacatatg atctggcgct gcgataacga tctggatatt
     3241 cagcagttgt tgactgccgc aatggatccg caggagttta cacgcttcag tcaggtctgg
     3301 caggaaaacg ggctggatca caactggctg ccgctgccgg tacatccgtg gcagtggcag
     3361 caaaaaatcg ccacagactt catcgctgat tttgccgaag gcaggatggt gtctctcggc
     3421 gagtttggcg accagtggct cgcccagcag tcgctgcgta ccctgaccaa cgccagccgc
     3481 cggggagggc tggatatcaa gctgccgctg accatctaca atacctcatg ctaccggggg
     3541 atccctggca gatatatcgc tgccggacca ctggcttcac gctggctgca acaggttttt
     3601 gcgaccgacg ccaccctggt gcaaagcggc gcagtgatcc ttggtgaacc ggctgcaggc
     3661 tatgtgtccc atgaaggcta tgccgcgctt gcccgggctc cctatcgcta ccaggaaatg
     3721 cttggtgtta tctggcggga gaatccgtgc cgctggctga aaccggatga aagtcctgtt
     3781 ctgatggcaa cactgatgga gtgcgacgaa aacaatcagc cgctggcagg cgcatatatc
     3841 gaccgctccg gactggacgc tgaaacctgg cttacgcaat tgttccgggt ggttgtggtt
     3901 ccgctgtatc acctgctttg ccgctacggt gtcgcgctta ttgcacacgg acaaaatata
     3961 acgctcgcca tgaaagaggg ggtaccacag cgtgttctgc tgaaagactt ccagggcgat
     4021 atgcggctgg tgaaagaagc gtttcccgaa atggactctt tgcctcagga ggtgcgtgat
     4081 gtcacatccc gtctgagtgc ggactactta atccatgatt tgcagacggg tcacttcgtg
     4141 acagtactgc gttttatttc gccactgatg gttcgtcttg gcgtacctga aaggcgattt
     4201 tatcaactgc tggcagcagt gttgagtgat tacatgaaca aacatccaca aatggcagag
     4261 cgttttgcgc ttttctcact cttcaggcca caaatcattc gcgtggtgct gaacccggta
     4321 aaactgacct ggccggacct ggatggcggc agtcgcatgc tgccgaatta ccttgagaat
     4381 ctgcaaaatc cgctgtggct ggtaactcag gaatatgaat catgaaaaaa agtgtcgatt
     4441 ttattggtgt cgggacaggg ccatttaatc tcagcattgc tgcattgtca catcagatcg
     4501 aagaactgaa ctgtctcttc tttgacgaac atcctcattt ttcctggcat ccgggtatgc
     4561 tggtaccgga ttgtcatatg cagaccgtct ttctgaaaga tctggtcagt gcagttgcac
     4621 ctacaaatcc ctacagcttt gttaactatc tggtgaagca caaaaagttc tatcgcttcc
     4681 ttacaagcag gctacgtaca gtatcccgtg aagagttttc tgactatctc cgctgggctg
     4741 ctgaagatat gaataacctg tatttcagtc ataccgttga aaatattgat ttcgacaaaa
     4801 aaagtcgatt gtttctggtt cagaccagtc ggggagaata ttttgcccac aatatctgcc
     4861 ttggtacagg aaaacaacct tatttaccac cctgtgtgaa gcatgtgaca caatcctgtt
     4921 tccatgccag tgaaatgaat cttcgtcggc cggacctgag tggaaaacgg ataaccgtgg
     4981 ttggtggagg acagagtggt gcagacctgt tccttaatgc attacgcggg gaatggggag
     5041 aagcggcgga aataaactgg gtctcacggc gtaataattt taacgcactg gatgaggctg
     5101 cttttgctga tgagtatttt acacctgaat atatttcagg cttctccgga ctgaaggaag
     5161 atattcgcca tcagttactg gatgagcaga aaatgacatc ggatggcatc actgccgatt
     5221 ctttactgac catttatcgt gagttgtacc accgttttga agttctgaga aaaccaagaa
     5281 atatccgtct gctacccagt cgctcggtaa caactctgga aagtagtggt ccgggctgga
     5341 ggttattgat ggaacatcat ctggatcagg gcagagagag cctggaaagt gatgtggtga
     5401 ttttcgccac aggttaccgt tctgcattgc cacaaatact tccctcactg atgcccttga
     5461 tcaccatgca cgataagaac acctttaaag tgcgtgatga cttcactctg gaatggagtg
     5521 gcccgaaaga aaataacatc tttgcggtca acgccagcat gcaaactcat ggcatcgccg
     5581 aaccccagct cagcctgatg gcctggcgat ctgcacgtat tcttaatcgc gtactggggc
     5641 gtgatttatt cgatctcagt atgccgcccg cactgattca gtggcgcagc ggcagccgga
     5701 aaaaaccaca gccggaggct gcttctttaa ctcactatac agcaaatatt caggaataac
     5761 gatgatgcgc aaaaagtata tgccccgggc tcttggtccg ctgcttcttg tcgtgctgtc
     5821 accagctgtc gcccagcaaa acgatgataa tgagatcata gtgtctgcca gccgcagcaa
     5881 tcgaactgta gcggagatgg cgcaaaccac ctgggttatc gaaaatgccg aactggagca
     5941 gcagattcag ggcggtaaag agctgaaaga cgcactggct cagttaatcc ccggccttga
     6001 tgtcagcagc cagagtcgaa ccaactacgg tatgaacatg cgtggccgcc cgctggttgt
     6061 cctgattgac ggtgtgcgcc tcaactcttc acgttccgac agccgacaac tggactctgt
     6121 cgatcctttt aatatcgacc atattgaagt gatctccggc gcgacggccc tgtacggtgg
     6181 cgggagtacc ggagggttga tcaacatcgt gaccaaaaaa ggtcagccgg aaaccatgat
     6241 ggagtttgag gctggcacaa aaagtggctt taacagcagt aaagatcacg atgagcgcat
     6301 tgccggtgct gtctccggcg gaaatgacca tatctccgga cgtctttccg tggcatatca
     6361 gaaatttggc ggctggtttg acggtaacgg cgatgccacc ctgcttgata acacccagac
     6421 cggcctgcag cactccaatc ggctggacat catgggaacc ggtacgctga acatcgatga
     6481 atcccggcag cttcaactga taacgcagta ctataaaagt cagggagacg acaattacgg
     6541 gcttaatctc gggaaaggct tttccgccat cagcgggagc agcacaccat acgtcagtaa
     6601 ggggctgaat tctgaccgca ttcccggcac tgagcggcat ttgatcagcc tgcagtactc
     6661 tgacagtgat ttcctgggac aggaactggt cggtcaggtt tactaccgcg atgagtcgtt
     6721 gcggtactac ccgttcccga cggtaaatgc gaataaacag gcgacggctt tctcctcgtc
     6781 acagcaggat accgaccagt acggcatgaa actgactctg aacagccaac tgatggacgg
     6841 ctggcaaatc acctgggggc tggatgctga gcatgagcgc tttacctcca accagatgtt
     6901 cttcgatctg gctcaggcaa gtgcttccgg agggctgaac aaccataaga tttacaccac
     6961 cgggcgctat ccgtcatatg acatcaccaa tctggcggcc ttcctgcaat ccagctatga
     7021 cattaatgat atttttaccg ttagcggtgg cgtacgctat cagtatactg agaacagggt
     7081 agatgatttc atcgactaca cgcagcaaca gaagattgct accgggaagg cgatatctgc
     7141 cgacgccatt cctggtggtt cggtagatta cgataacttt ctgttcaatg ctggtctgct
     7201 gatgcacatc accgaacgtc agcaggcatg gttcaatttt tcccaggggg tggcattgcc
     7261 ggatccgggg aaatattatg gtcgcggcat ctatggtgca gcagtgaacg gccatcttcc
     7321 cctgacaaag agcgtgaacg tcagcgacag taagctggaa ggcgtgaaag tcgattctta
     7381 tgaactgggc tggcgcttta tcggtgacaa cctgcggact caaatcgcgg catattactc
     7441 gctttccaat aagagcgtgg aaaggaataa agatctgacc atcagtgtga aggacgacag
     7501 gcgccgtatt tacggcgtgg aaggtgcggt ggactacctg atcccggata ctgactggag
     7561 taccggtgtg aacttcaatg tgctgaaaac cgagtcgaaa gtgaacggtc aatggcaaaa
     7621 atatgacgtg aaggaatcaa gtccatcgaa agcgacagct tacattaact gggcgccgga
     7681 accgtggagt ctgcgtgtac agagcaccac ttctttcgac gtaagcgatg cagagggtaa
     7741 cgatattaat ggttacacta ccgtcgattt tatcagtagt tggcagcttc cggtgggaac
     7801 actcagcttc agcgttgaga acctcttcga ccgtgactat accactgtct ggggacagcg
     7861 tgcacctctg tactacagcc cgggttacgg ccctgcttca ctgtacgact acaaaggccg
     7921 gggccgaacc tttggtctga actactcagt gctgttctga cggggatgct tttgcaacaa
     7981 gggtatgctg taaacaacat ggtccttggt ggccatgttg tttacagcca gtataatata
     8041 cagccttaac aagatattcc gtctccggaa tgggctgttc accactgaat aacattatgt
     8101 ctgttttatg gatctttgac tgaaatttat cgcatatcct catttcattg tccgtctgaa
     8161 aactaacctt acaagtaagc gtcgtctcag caccgtctgg cagatcctga aattcctgag
     8221 aggatgatct gcgcccaata tcctgagccg gaactttgct gcaatgaagg ccaacgaaaa
     8281 atgggggagt atgttataga gtacttgcta cagggtaaaa agaggtacct gtcgctggtt
     8341 ctccgttcag tttaaccggg agattttctc ctgcgcctgt cataaagtcc ggtaatggag
     8401 attgttaata cgatggtgca cgatgcatcg tcagtgcccg gcccggagga tgccccgtag
     8461 ctacatacgg atcagggttg gcaatatcga atggcaggct ataggcaaag ttaaagcccc
     8521 atggagtagc acaaaatatg ccgcacaaag gaaacggtct gaataacgca gtgatggaga
     8581 acttcttcgg cacactgcaa aatcgcatcg tggtcgatcg ctaattcgtc gtgaatcact
     8641 gaacgatgtt ccatgcgaac agatattcga tcatatcgaa atattaaaca ttacccctcc
     8701 ccacattggg caaaaaaaaa caaaaaacga tcaattagta ttgattcaca taatctctca
     8761 tcattaatga gaacattgtc acatatttac aatatagaac tgttactttt agcaagctgt
     8821 tagtaaccaa ccgttactaa taattttaat tcttaatact ttgttcattc aataaatgag
     8881 ttgagagaat atatgaataa aatatactcc cttaaatata gtgctgccac tggcggactc
     8941 attgctgttt ctgaattagc gaaaagagtt tctggtaaaa caaaccgaaa acttgtagca
     9001 acaatgttgt ctctggctgt tgcaggtaca gtaaatgcag caaatattga tatatcaaat
     9061 gtatgggcga gagactatct tgatcttgca caaaataaag gtattttcca gcccggagca
     9121 acagacgtaa caatcacttt aaaaaacgga gataaattct ctttccataa tctctcaatt
     9181 ccggattttt ctggtgcagc agcgagtggc gcagctaccg caataggagg ttcttatagt
     9241 gttactgttg cacataacaa aaagaaccct caggccgcag aaactcaggt ttacgctcag
     9301 tcttcttaca aggttgttga cagaagaaat tccaatgatt ttgagattca gaggttaaat
     9361 aaatttgttg tggaaacagt aggtgccacc ccggcagaga ccaaccctac aacatattct
     9421 gatgcattag aacgctacgg tatagtcact tctgacggtt caaaaaaaat cataggtttt
     9481 cgtgctggct ctggaggaac atcatttatt aatggtgaat ccaaaatctc aacaaattca
     9541 gcatatagcc atgacctgtt aagtgctagt ctatttgagg tcacccaatg gga
//
LOCUS       DAZPAB010000147         9560 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZPAB010000147 DAZPAB010000000
VERSION     DAZPAB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9560
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.147"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="ACHUPG_003073"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="ACHUPG_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR0862591.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1102..1185
                     /gene="yqiM"
                     /locus_tag="ACHUPG_003074"
     CDS             1102..1185
                     /gene="yqiM"
                     /locus_tag="ACHUPG_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR0862592.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1207..1869
                     /gene="yqjA"
                     /locus_tag="ACHUPG_003075"
     CDS             1207..1869
                     /gene="yqjA"
                     /locus_tag="ACHUPG_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR0862593.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="ACHUPG_003076"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="ACHUPG_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR0862594.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /gene="yqjC"
                     /locus_tag="ACHUPG_003077"
                     /pseudo
     CDS             2403..2757
                     /gene="yqjC"
                     /locus_tag="ACHUPG_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
     gene            2795..3100
                     /locus_tag="ACHUPG_003078"
     CDS             2795..3100
                     /locus_tag="ACHUPG_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR0862595.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="ACHUPG_003079"
     CDS             3103..3507
                     /locus_tag="ACHUPG_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR0862596.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="ACHUPG_003080"
     CDS             3497..3796
                     /locus_tag="ACHUPG_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR0862597.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4278
                     /locus_tag="ACHUPG_003081"
     CDS             3982..4278
                     /locus_tag="ACHUPG_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR0862598.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4348..5334
                     /gene="yqjG"
                     /locus_tag="ACHUPG_003082"
     CDS             4348..5334
                     /gene="yqjG"
                     /locus_tag="ACHUPG_003082"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFR0862599.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5627..5995
                     /locus_tag="ACHUPG_003083"
     CDS             5627..5995
                     /locus_tag="ACHUPG_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR0862600.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="ACHUPG_003084"
     CDS             complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="ACHUPG_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFR0862601.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7203..7904
                     /locus_tag="ACHUPG_003085"
     CDS             7203..7904
                     /locus_tag="ACHUPG_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR0862602.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7927..8091
                     /gene="yhaL"
                     /locus_tag="ACHUPG_003086"
     CDS             7927..8091
                     /gene="yhaL"
                     /locus_tag="ACHUPG_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR0862603.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8303..>9560)
                     /gene="cyuA"
                     /locus_tag="ACHUPG_003087"
     CDS             complement(8303..>9560)
                     /gene="cyuA"
                     /locus_tag="ACHUPG_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR0862604.1"
                     /translation="EEVKPALGCTEPISLALAAAVAAAELEGPVERVEAWVSPNLMKN
                     GLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQAITDAKALLAAGKVSVKI
                     QEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNGVVFTHQACVAEGEQESPL
                     TVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEGLSGKWGLHIGATLEKQCE
                     RGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQGITAIMPVVVVAEHFGAD
                     DERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAAGMAWLVDGRYETISMAIS
                     SMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDTAVTGNEGIVAHDVEQSIA
                     NLCALASHSMQQTDRQIIEIMASKAR"
BASE COUNT         2369 a         2509 c         2388 g         2294 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatat ttccgccagt agtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtaga gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc atcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cggccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     5701 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     5761 ctggcaacgc gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt
     5821 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     5881 actgctattc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     5941 gggtacgcca ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt
     6001 aagcaccttg taaaaggtgc ttcaaattgt tgacaaagtg cgctttgttt atgccggatg
     6061 cggcgtaaac gccttatcca gcctacaaat tgccaaaatt caatatattg cagtgactgc
     6121 gtaggcctga taagcgtagc gcatcaggca gttttgcgtt tgtcagcagt cagaagcacc
     6181 ttgtgaaagg tgcttaatag attattttcc agcgaacaac tttggaattt cccgcagaca
     6241 ccaggatttc gcttccccca tactgtcacg acgccaggcc ataataatat cgatctcgct
     6301 ggtcgattcc gggctgacga cacgcaaccg cccttccgca atatcttttt cgaccatcgg
     6361 atacggcatc gtcgccacgc caagccccgc cagtaatgcc tgacgtttat cttcaatcgt
     6421 gctcaccgtt aagcgcggct gtttgtccag cagctgtacg gtcaacaccg ggcgctcacg
     6481 agcggtatcc gctaccgcaa ttccacgata tttcacacgc gtcacttcag ataacggttc
     6541 cggctcctga tgaatcgggt gatcaggcgc ggcaacgtag acgttcatta acgtatagag
     6601 cttgcgcgag ttgatctccg acgaggaacg aaaatgcata tccggcgcga taacgatatc
     6661 cgcccgcccc tgctccagcc gttcccacgc ccccgccagc acttctgtga tgattgccag
     6721 ttgggtattg gcttttgccg ccagtttgtc gattaacggg aaaaaggcag gtgtcggtac
     6781 cagcgcttcg gtcacaatgg tgagatgcgt ttcccaaccg cgcgcgagag cttccgcatc
     6841 agtagtcagt ttatctgcgg cttccagcaa aacgcgccct cgctccagca acatcctccc
     6901 gacattggtg aatttggtgc gatggcccga gcggtcaaac agcaccacat ccagctcttc
     6961 ttccagtttt tgcatggtgt agctaagtgc ggaaggcacg cgtcccagct catccgctgc
     7021 cgccgcaaaa ctgccccggc gatcgatcgc atccataacc cgtagtgctt ccagcgttaa
     7081 tgccctttct ttggccattt cgttctcatt caggaaattt gaacataccg ggcagaatat
     7141 ctggctaaca atgtagcgtc cagcccctta ccataaaagg aagtaaagag aggtcaagaa
     7201 ttatgattac tacccgaact gccaggcagt gtggacaagc agactacgga tggttgcagg
     7261 cccggtatac tttttccttt ggacactact tcgacccgaa attgttaggc tatgcctccc
     7321 tgcgtgtgct taaccaggaa gtgctggccc caggtgccgc ctttcagccg cgaacctatc
     7381 ccaaagtcga tattttaaat gtgattctgg atggagaagc agagtatcgc gacagcgaag
     7441 gcaatcatgt tcaggccagc gccggagagg cgttgctgct ctctacccag ccgggtgtca
     7501 gctatagcga acacaatctc agcaaagaca aaccgttaac gcgaatgcag ctttggctgg
     7561 atacctgccc gcagcgagag aatccgctga ttcaaaagct ggcgcttaat atggacaagc
     7621 agcaattaat cgcctcgcca gagggtgcga tgggaagcct gcaattacgc caacaagtgt
     7681 ggctgcacca tatcgtgctc gacaaaggcg aaagtgcgaa tttccagttg catgggccac
     7741 gcgcgtattt gcaatcgatt cacgggaaat ttcatgcgct tacgcatcat gaagagaaag
     7801 cggcgctaac ctgcggtgat ggggcgttta ttcgtgacga ggctaacatt acgctggttg
     7861 ccgattcccc actgcgcgct ttgctgatag atttgcctgt ctagttgttt ttacagggag
     7921 atgatgatga gtaaaaaatc gaccaaaaag cgccagccag cgaagcccgt ggtggcgaaa
     7981 gaacctgctc gcaccgccaa aaattttggc tatgaagaga tgttgagcga gctggaagct
     8041 atcgtcgcgg atgctgaaac gcgtttagcc gaggatgaag ctaccgcgta aagacaggtt
     8101 gccggatgcg gcgtaaacgc cttatccggc ctacaaaaca caacaacatc aacaaattaa
     8161 acaccccgta ggccggataa gatgcgccag catcgcatcc ggcatcaggt acaaaacaca
     8221 acaacatcaa caagttgagc accccgtagg ccggataagg tgcgccagca tcgcatccgg
     8281 cacaatactc aaaacctggc gtttatctgg ccttgctcgc cataatctcg ataatctgcc
     8341 gatccgtttg ctgcatcgaa tggcttgcta acgcacacag gttggcaatc gactgctcaa
     8401 catcatgcgc cacgatccct tcattgccgg tcacggcggt atcatccagc gccattaaca
     8461 ccgctttcca cgcagccgaa gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac
     8521 catcgcaaat catgccgctg acatcgccga tcatactgct gatcgccatc gagatagttt
     8581 cataacgccc atccaccagc catgccatcc cggcggcggc ccccattgct gcggtcgttg
     8641 cggcacaaag tgcagacaaa cgcggtaact ggttatggat gtaaattgcg ctcaaatgag
     8701 aaagcatcag cgcacgcgcc agtcgttcat catccgctcc gaagtgttct gctacaacca
     8761 ccacaggcat tattgcagtg atcccctggt tacccgagcc ggagttactc atagccggaa
     8821 gcgtagcgcc gcccatacgc gcatcggatg ccgcgctggt acgaatcaca atggatgaag
     8881 agagatcttt cgccagcaaa ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca
     8941 gcccccactt accgctcaaa ccttcctgcg ataacgcaca atttagcttc gcggaatcga
     9001 gaataaagcg gatcgccgca aatgggactt cattgacgaa cttcaggatc tcagccagcg
     9061 tcgttctgga aagcaccgtc agcggcgact cttgctcgcc ctctgccaca cacgcctggt
     9121 gggtaaacac cacaccattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga
     9181 tggtgacaca cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat
     9241 tgcaaggttc ctggatctta acagagactt tccccgccgc cagcagtgct ttggcatcgg
     9301 taattgcctg cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta
     9361 acgcccccag cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc
     9421 ccagaccgtt cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt
     9481 ccagttctgc cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca
     9541 gcgccggttt tacttcctcc
//
LOCUS       DAZPAB010000148         9537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZPAB010000148 DAZPAB010000000
VERSION     DAZPAB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.148"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(54..533)
                     /gene="xdhC"
                     /locus_tag="ACHUPG_003088"
     CDS             complement(54..533)
                     /gene="xdhC"
                     /locus_tag="ACHUPG_003088"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR0862605.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="ACHUPG_003089"
     CDS             complement(530..1408)
                     /gene="xdhB"
                     /locus_tag="ACHUPG_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR0862606.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="ACHUPG_003090"
                     /pseudo
     CDS             complement(1419..3716)
                     /gene="xdhA"
                     /locus_tag="ACHUPG_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            4131..4886
                     /gene="actS"
                     /locus_tag="ACHUPG_003091"
     CDS             4131..4886
                     /gene="actS"
                     /locus_tag="ACHUPG_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR0862607.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4965..5038
                     /locus_tag="ACHUPG_003092"
     tRNA            4965..5038
                     /locus_tag="ACHUPG_003092"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4997..4999,aa:Gly,seq:ccc)
     gene            5173..6783
                     /locus_tag="ACHUPG_003093"
     CDS             5173..6783
                     /locus_tag="ACHUPG_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0862608.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6815..7234
                     /locus_tag="ACHUPG_003094"
     CDS             6815..7234
                     /locus_tag="ACHUPG_003094"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862609.1"
                     /translation="MVKEFLVSKENKPSYECMNTAETCLGLAGMASEDWQREHHCRTG
                     IIFTAFAIEAMFIFYRKQVDPGYDKTQKECRKTMHKNTLKLCGINNYMGTKPYQIIKE
                     CLEVRDAIAHGDSYTSSFNFSADHLDNQDDIAKVSWR"
     gene            8353..9099
                     /locus_tag="ACHUPG_003095"
     CDS             8353..9099
                     /locus_tag="ACHUPG_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR0862610.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
     gene            9397..>9537
                     /locus_tag="ACHUPG_003096"
     CDS             9397..>9537
                     /locus_tag="ACHUPG_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862611.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLP"
BASE COUNT         2432 a         2362 c         2422 g         2321 t
ORIGIN      
        1 tgttaaacac ataacctcag tgaatccggc ctgaattcag gccggatatc cttttacttc
       61 gttttctcgc aatccagaac tgtatttaca atcatctgat accccgtgca gcgacaaaga
      121 tttcccgcca gtccgcgacg aatttccgta atggttaatg gcttctcgcg tggtttcgcc
      181 agcattgccg tggtagccat aatcaggcca ggcgtacaaa acccgcactg cactgtgccg
      241 gatttcgcat aagcctgctg aacatgagaa agttttccgc ctttcgcttc accttccagc
      301 gtgcggatct cttttccttc agcccaggcg gcaaggtata agcaactgtc tattgctgtg
      361 ccgtcgacca acaccgtaca ggcaccacat tcacccacgc agcacccttg tttgacactt
      421 agcagtcctt gttcgcggag taattccgag agcggcgtgc ctggtgcggc gtgaagctga
      481 aaaggcatcc cgttaatggt gcattcggtg gtaattgttt cgctgtgatt cattgcaatt
      541 ttccccccgc cgcggcgacg gcttcgctaa tcactttttt ggtcatcgtc tggatgagat
      601 gcagacgaaa ctctttactg gcccgccatg aagaacgcgg ggcgacatct tgcaggacag
      661 attcgctgat agcttccagc gtttgcaggt ttaatggcgc attttgtgca gtctgttcgg
      721 catgttggca gcgaatcggc gttggcgcgg caacaccaaa tgccaggcgt aattcgctga
      781 aattgccgtt atccagtcgg caatgtgcgg cgcagccaat cgttgaaata tccattgcgt
      841 cgcgcatggc atatttaaaa tgcgcgctgc ccgcgtgttc tttcggctgt ggcggaaaat
      901 gaaaggcgac gaggatttcg tcatgctcaa gagacacttt gcccggcccg gtgtgaaagc
      961 cattaatcgg gacgaaacga acaccgcgcg gggagtggat ctccagtttc gcgtcataaa
     1021 ttagcgttgg cgtggcagaa tctgcgctgg tggcaccgtt gcaaatattt ccaccgtagg
     1081 tagcgacgtt acggatctgc ggtccagcaa tgaacgtggc cgcagcacat aacgccggga
     1141 gatgacgttg agttatagga tcttctatta gctgggtaaa tgtcgttgca gagccgatac
     1201 gtagcgagcc atcttccgcc agcgtaattc cccgcagctc cgccagatta tgaatatcaa
     1261 caatatggcg ataacggtca ttgtgatggt ggagctgaat cagtacgtca gtgccaccgg
     1321 cgagcagttt ggcctgcggg ttgtcagcca gcaggttgat ggcatcggca agggttgctg
     1381 cgcgatggta agaagcaaaa tcaaacatga tgttatcctc aaatcaatcc tgccagatgg
     1441 aactcttcat ataaccgttt tggcgtcagc ggcagtgtat tgattgcaac accggtagcc
     1501 atcttcaccg cgttacgaat agcagcggca acaggaatta tgggtggctc acccagtgac
     1561 ttatgtccgt atgcggattg cggctcattg atttcgacga acgcgctttc cagttgtggc
     1621 agatccggca tggtcggcat tttgtaatcc agcagattgg ggttacggac cacgccgctt
     1681 ttagcatcga tgatcatctc ttcaaatagc gcccagccaa tgcccattcc cattccgccg
     1741 tgtacctgac cttctgccag cagtggatta agaatatgcc ctgaatcatg aacgttgagg
     1801 atgcggttga tggtgacttt gcacagcgca atatcgaccg tcagatcaac aaaggtacag
     1861 ccaaacgccg gtgggttagt ggtggttttg atggagcttt cagcagagag ctgcccgccg
     1921 cgttcagggt ggtagaaagc gtccatcgcc aaatctttta acgacattaa cggctcttcc
     1981 ggtcgttcaa ccagcacgat atggcctttt atcagggtca aattcatcgc tgactgatgt
     2041 agcatgactg cggcgtgagc gatgattttc tcttttaata atagtgccgc actgcgcagc
     2101 gcaggcgcgg caacatagct ctggcgtgag gcaaatgcgc cgggatcgaa cggcgtaacg
     2161 tcggtatctt gtgttgaaat aacgcgaacg tcgctgaccg gaacccccac ggtttctgcc
     2221 accatttgcg agaagacggt gtcggcaccc tgaccgattt ccgtcgcgcc gctttgcacg
     2281 ttgatggttc catcctgatt catcagaagg cgcgcgcctg ctatttctac gccgacaggc
     2341 caggtgttag aggtgtagct aaaacaggcg acgccaacgc cgcggcgcaa attgccttgc
     2401 tggttctggc attctgcacg gcgtttttcc cattcaaaga ttttccggcc tttttcaaga
     2461 cactccggca accctgcgct gcaaatacgt ttgcccgtga gcggattagc atctccttcg
     2521 cgggcggcgt tgcgtaaacg aatttcaaca ggatcaatac ctaacgctgt cgcggcgtca
     2581 tcaagcatag actcaacggc aaatacgact tgtggcgcgc cataaccacg catcgcacca
     2641 gccgagggga ggttggtata gcaggtcttt gaactgtaag cgtaggcaca acgaggataa
     2701 aggtaagcga ctttattccc ccccgcagaa gcgatggagt gcccgtgaga tgcataagcg
     2761 ccggtgttag acagaacatc cagactataa cctttcaagg ttccgtcgcg gttcacgccc
     2821 atttgcccgt caatagtaaa agcgtggcgg gtacgggttg cgaggaaaca ctcttcacgg
     2881 ctaagggaaa ctttcaccgg aatgccgcca agcttgctgg tcaggaatgc cgccattggc
     2941 tcttccagta catcctgttt attaccaaaa ccgccaccga caaacggttt gatgactcgt
     3001 acgcatgacc agggaatatc cagcgcctga ccaaccacgc ggcgaacaat gtgcgggatc
     3061 tgggtgctgg aaacgatggt aattcgcgag tcatcctcca tccacgccag cgatgttacg
     3121 ctttccatgt gacaatgttg aataacggga gtctgatagt gcccctgtac ctggtagtcg
     3181 gcggcatcga ttgtttgttg gacattgccc gtcgacatcg tgctttgttt cagtaaattg
     3241 ccgccgttat ggattggtgc agcgtcttct gtcagcgccg cttctggcgt ggtgataacg
     3301 ggtaattctt gccactcaat gctgaccaat tgcgccgctt tttctgccgt gagttcatcg
     3361 cgggccacga cgatggcaac ggcgtcacca tgatgacgaa catggcgagt tagcagtgcg
     3421 cgatcggcgg tatcgcgctt gttttcgtca agtgtctagg catgcccagc tgtagcgaat
     3481 ggaatatcag gcacatcttc ccaagtaaaa atcgccagta cgcccggtaa acttctggct
     3541 tgttcatcat taatacttac ggcataacca tgtgcgatag ggctacgtac atatttcgcg
     3601 taacacatgc ccgccataac ataatcgtca gtatatcgtg cccgcccggt gaccttagca
     3661 atggcatcga cgcgcatgca tgattcaccc gtagcggttg cttcccgcgc ttccatagaa
     3721 atcccctcga tttgaacatt tggttcttat ggcaaagatt tagtctaagc aggcaagatt
     3781 tacgccagaa acgacgataa gcgttagggg aggaaggaag tgtgatgacg ttaaaaaaac
     3841 acagatatga tacgaaacag gactctgaat agggacagtg atacgtatca atatgagtgg
     3901 cgataaaatc aatgtgataa atcaggatta ttacgcatca ggatgggaaa tagggtaaaa
     3961 agtcaggaac ttcgatacct ctacagcgaa tattttgctg atgatacttc cctttgccaa
     4021 atgaaattaa cctctttctc tattaccacg tttttccaga agcaagagat tgggtatcaa
     4081 gaggctggct gctatgatat ggcgtcttgt tttttaagcg aggaaagatt ttgagtgcgg
     4141 gacgcctgaa taaaaaatct ctgggtatcg tgatgttttt atcggttgga ctgctattgg
     4201 cgggctgttc gggtagcaaa tcatccgata caggaacgta ttccggctcc gtttacaccg
     4261 tgaaacgggg ggatacgcta tatcgtattt cgcgcaccac gggaaccagc gtaaaagagc
     4321 tggcgcgact gaacggcatt tccccccctt acaccattga agttggtcag aaactaaaac
     4381 tgggtggggc gaaaagtagc agtagtacac gtaaatcaac cgccaaatca acgactaaaa
     4441 ccgcatcggt tacaccgtca tcagcggtac cgaaatcttc ctggccgcca gtagggcaac
     4501 gttgttggtt atggccaacg acagggaaag ttatcatgcc gtattcgaca gcagatggcg
     4561 gcaataaagg gattgatatc tcagctccac ggggtacacc tatttacgcc gcgggtgcag
     4621 gaaaggtggt gtatgtgggc aaccagctgc gtggctacgg taatctcatc atgattaaac
     4681 acagtgaaga ttacattacg gcttacgccc ataatgacac gatgctggta aataatgggc
     4741 aaagcgtgaa ggctgggcaa aaaatcgcca ctatggggag cacggatgcg gcatctgttc
     4801 gcctgcattt ccagattcgt taccgtgcaa cggcaattga tccgctacgt tacttgccgc
     4861 cgcagggcag caagccaaaa tgctgatggc gaattaatca gcagtcagca gcgcggcact
     4921 tgctaaggag agcgtaaggt ttataatgcc ttacgcatct cgaagcgggc gtagttcaat
     4981 ggtagaacga gagcttccca agctctatac gagggttcga ttcccttcgc ccgctccatg
     5041 acacttctta aagtctacta acttactgaa tcctaaggga tacggcttaa cgccttccga
     5101 tagttttact cactgtaacc aacactgtga ctaactttgt tgaagtagtg agcagaccta
     5161 aggtctcagt acatgctcaa gtcaagaacc tacctttatc agcgtaatgg tgtcttttac
     5221 atccgtctta ggatgaagac cactagtcgc ctgaccgctt cgcttccttc tcacaaccgt
     5281 tataaactag cgtcagtatc tttacggacc aaggatagac gtaccgctat ggctcactca
     5341 cggcacatca agtcagcact taaagcaatc catgcagaca accctaacgc ctcttatgag
     5401 gagctacggg agcacctgaa gaccattgtc gagtgggaac ttagtgtcag ccgtgatgac
     5461 ctgaacgacc cggagtccta ccagctttac gttgaccagt acgatgacat taagtccaac
     5521 cttcgggaag ctgtagcgac cgagcgtctg actgtagacc agcatcgcta tattaatgac
     5581 gttatcggtg tgcttaaggc ctgtcaggat agactcaatg gcgatagctc tggcctattg
     5641 tcttaccttg agcctgagac tggcagtcta agaccgtccg tctccctatc tgtattggcg
     5701 gaacctgaag tccctgaacc taaagcactg accctcgcgt ctctcattga gcagtacgag
     5761 caggagaacg cccagaactg gaagccagcg accctaagcg aaaaccgagc gtcacactct
     5821 acgctaatcg agatattcga ctatctggat attcaggatg tcggcaaggc gacccgtgca
     5881 gatatgctca gagttcgtga agtcctccag cagctaccga agaaccgcaa gcaacgcttt
     5941 aagtctatgc cgctgtctga cttgctgaac cgggagtcta agactgactg tctggacgtc
     6001 gttaccatta acaataaata tcttatcaag atggctgctg tgtttaagtg ggcggtacgc
     6061 aacgacttga tagctaagaa cctgaccgaa ggtcttgagc tgaaagtccc tcagcgtaaa
     6121 gcctccgacg ctcgtgatgc gttcagtcct gagcaggtag ggcaactact ggtcgcagct
     6181 aaagcgtact cgcagaagac ctcaggtaag ccataccatt attacgtcac cgctctggct
     6241 gcgattacag gtgcgagact taacgaggtg gcccaacttc aggttaaaga tgtcaggacc
     6301 actgaggctg ggaccgtttt tatccatatc aacgaagacg atagcagcct gccgggtaag
     6361 agtatcaaga acgcccacag tgaccgctgt gtcccgttgg tagatggtgc ttatggtttt
     6421 gtattggctg actttatgtc actcgtggaa gaccgtagaa agactgaggg tgataatgct
     6481 atggtgttca atggcctcaa gctaatgaag aacggctacg gtgagcaggt tagcaagtgg
     6541 tttaaccgaa cgttgctgcc caaagtcctc gctgaccgta gcggcttagc ttttcactcg
     6601 tttagacata cagttgcgac ccagttaaaa caacatgggg tcgagttagc gtatgctcag
     6661 gcgattatgg gtcatagctc agggtctata acttacgacc gttacgcgaa agaggtcgag
     6721 gtggaaacgc tgaaagaaaa attagcggag tcattgtcag taaaaaaaat agacggaaag
     6781 taatcgcata gggatagcgg aatgtgagga cgtaatggtc aaagaatttt tagttagcaa
     6841 agaaaataag ccttcatatg aatgtatgaa tactgctgaa acttgtttgg ggctggctgg
     6901 aatggcttcg gaagattggc aacgtgagca tcattgccgc acaggtataa tatttactgc
     6961 ttttgctatt gaggcaatgt tcatcttcta tagaaaacag gttgaccccg gctacgataa
     7021 gacgcagaaa gagtgcagaa agacaatgca caagaatacg ttaaaattgt gcggtataaa
     7081 caattatatg gggacaaaac cttaccaaat tatcaaagag tgtcttgagg taagagacgc
     7141 tattgctcac ggcgattcgt atacttcgag ctttaatttc tcggcggatc atttggataa
     7201 ccaagacgat attgcaaaag tgtcctggcg gtaaactctg aacaatttcg gggattaact
     7261 gttgagcgcc ttgcaaaata cattgaaatg gcaaagcata ttgaccatga gatttctgat
     7321 aatggttaca ggccttctga aggttacctt ccagcagccg agcgcagaag tctgatgtta
     7381 gcttttggcg tcagtggtgt gtcaatgtgg cccagtgctt gagcgataat acacctcact
     7441 atagaggaat ggacccttaa ctatcactat aggggagaga ccctaagtcc ccaccttaat
     7501 agcagtcacg acccatacac acattcatta agtctcaggc ttccggtagc ggaccgattg
     7561 gaaaccatcc gcagacgcct agtgttaaat caccttggcc tgagaccatt ccttttaacg
     7621 taagctatct tcccttttga caggcgggga gcagtagacc acatccgact ttaagttggc
     7681 tctgtgataa tcgctgcgcg gataactgaa gaaccaagac accattactg aggtctgcca
     7741 tccatttgtg gacaggcgga aactcaaaga ggttagatca gtggtgtctg aggttggagc
     7801 tttgagtctt gaccagcagg ctggaggaga ccatgagttt atcttgttat ggattgtgtt
     7861 atcacaattc caataagaag aactttaagt ccagcctcca ctcccgccaa cccgaagtaa
     7921 aattatggtc gctatgatgt ggtctccatg cttcgggtct tactggaagt actgaacact
     7981 ttcgcgtcaa gttctccgaa gttcattcct gtttgactaa actatcacta tagggaccat
     8041 atctcgcctc tgacctcctg agagacggca agcgagaaac cgtggtccac gctagcaagc
     8101 tagccaccag cctaaggcaa gacagcctct ggtcagcctg tgtgttcttt ggtctcgtga
     8161 cttaagtcta tcaccacaca cgctcggaca tgctgtcgaa ccttcatctc tatggtcatc
     8221 gcagaccact aaccccaatt acctcaagtc attctgtttg cgtcctgttg cctgctggtc
     8281 tccctttacg tgggggcttg agtaggtcag tggggtgcga cttcagggtg caaacaaaag
     8341 gagtacacgt aaatgtccca acaagaaatc agcatcatta acctcgacca gcttgtgtcc
     8401 atgacctcag ttgaaattgc tgagctgaca ggaaaagaac ataaacacgt tttacgagat
     8461 attcgtaata tggtcgaaga gcttaacggg gccaaaactg agcactgctc aactttgagc
     8521 agcgagctta acgggtccaa atttgggctc gttggcgagg aagtatacaa ggacgcgaaa
     8581 ggtgagagcc gaacaatgta tcgtctcgac cgtaagcata ctttcattct ggtcgctggc
     8641 tattccgttc acctccgcgc caagtgttac gaccatattc agacgttaga gcgtcgagtc
     8701 cttcaactgg aagaccagaa gaagcgagca gccattcagt ccgctaaccg ccgaggcgtg
     8761 acttggggcg actactgcaa aacctatggt ctgcccgctc agaagctaat gaccgcactt
     8821 ctccagcatc gtggcctctt tcgtaagaac ccaatcagta acgagtggtc tgtaaatccg
     8881 aagtattccg actgctttcg tatcattaag ccgtcagacc agaagttctc agcaggtggg
     8941 tataacttcc ggttcaacgc taaggggctg gaggtcttcg gtaagcctga gatggttgac
     9001 aagatgcgag gcatccttat tgcattcact ggcaccgacc agcagaagca agagcacctc
     9061 ctgaagctgg cacaatccgg taaggtggag ggaatctaag atgatgattg acccgcgcac
     9121 acctgagggg cgcatgactc tccgttaccg tggctaccgg actgaagtcc tcctcagaga
     9181 gcttggtcta gacccggaag atgagacccg ccagcaccaa tcccgagacg agctaatagc
     9241 ccaactggtc gctatgaaac tgccgcttaa ccgttagtag ctcacctact caatacctaa
     9301 tttaaacacc ttttgagatg accgatgcgg ggagacccgt aggccttact gtgcctgcga
     9361 taccgatagt ctcaactctc aagaggagaa ttacctatga tgttcaacaa caacaactgg
     9421 aagctgtcag tcaccgacat caacctttat gaaaacactg tgagtctgga cggtcagtcc
     9481 tatccgttgt cgctcgctat caagaccctc attccgggct acctgtcagg cttaccg
//
LOCUS       DAZPAB010000149         9494 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZPAB010000149 DAZPAB010000000
VERSION     DAZPAB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.149"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..190)
                     /locus_tag="ACHUPG_003097"
     CDS             complement(<1..190)
                     /locus_tag="ACHUPG_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR0862612.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            710..1405
                     /gene="minC"
                     /locus_tag="ACHUPG_003098"
     CDS             710..1405
                     /gene="minC"
                     /locus_tag="ACHUPG_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR0862613.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1429..2241
                     /gene="minD"
                     /locus_tag="ACHUPG_003099"
     CDS             1429..2241
                     /gene="minD"
                     /locus_tag="ACHUPG_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR0862614.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2245..2511
                     /gene="minE"
                     /locus_tag="ACHUPG_003100"
     CDS             2245..2511
                     /gene="minE"
                     /locus_tag="ACHUPG_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR0862615.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2883..3227)
                     /locus_tag="ACHUPG_003101"
                     /pseudo
     CDS             complement(2883..3227)
                     /locus_tag="ACHUPG_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3262..3381)
                     /locus_tag="ACHUPG_003102"
     CDS             complement(3262..3381)
                     /locus_tag="ACHUPG_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR0862616.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3342..3527)
                     /gene="ymgJ"
                     /locus_tag="ACHUPG_003103"
     CDS             complement(3342..3527)
                     /gene="ymgJ"
                     /locus_tag="ACHUPG_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR0862617.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3628..3801
                     /gene="ymgI"
                     /locus_tag="ACHUPG_003104"
     CDS             3628..3801
                     /gene="ymgI"
                     /locus_tag="ACHUPG_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR0862618.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3803..4147
                     /locus_tag="ACHUPG_003105"
     CDS             3803..4147
                     /locus_tag="ACHUPG_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR0862619.1"
                     /translation="MKKKILAFGLISALFCSTPAIADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4151..4486
                     /locus_tag="ACHUPG_003106"
     CDS             4151..4486
                     /locus_tag="ACHUPG_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR0862620.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4543..6864)
                     /locus_tag="ACHUPG_003107"
     CDS             complement(4543..6864)
                     /locus_tag="ACHUPG_003107"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0862621.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6981..7190)
                     /locus_tag="ACHUPG_003108"
     CDS             complement(6981..7190)
                     /locus_tag="ACHUPG_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862622.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7590..7809)
                     /gene="ymgF"
                     /locus_tag="ACHUPG_003109"
                     /pseudo
     CDS             complement(7590..7809)
                     /gene="ymgF"
                     /locus_tag="ACHUPG_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
     gene            complement(7941..9464)
                     /locus_tag="ACHUPG_003110"
     CDS             complement(7941..9464)
                     /locus_tag="ACHUPG_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR0862623.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2660 a         2206 c         2079 g         2549 t
ORIGIN      
        1 cggctttttc gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac
       61 aaatatacgg gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc
      121 cagacgccat tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca
      181 tcatattcat agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc
      241 aatccgtaat caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac
      301 gtcttctgcg gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct
      361 gttctttaat gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata
      421 cacttgcctc aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg
      481 gaaaaaggaa gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag
      541 ggtatttttt aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata
      601 aatgggaggg tgacttgcct caatataatc cagactataa catgccttat agtcttcgga
      661 acatcatcgc gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac
      721 tccaatcgag cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga
      781 acctaaggtt atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa
      841 acatgccccc gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat
      901 gcataaggcg gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc
      961 gcaacttaaa gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa
     1021 agcgccacgt ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa
     1081 aacgcgttta atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga
     1141 tctgattgtt acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca
     1201 tgtctatggc atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca
     1261 aatattttgt acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag
     1321 tgatcaaatc ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc
     1381 tttgaccgtt caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt
     1441 attgttgtta cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc
     1501 actggtttgg cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt
     1561 aatctcgacc tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt
     1621 cagggcgatg caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat
     1681 attcttccgg catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa
     1741 gttcttgatg atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg
     1801 attgaaaccg gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac
     1861 ccggaagtct cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca
     1921 cgccgcgcag aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac
     1981 ccaggccgcg taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc
     2041 atcaaactcg tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt
     2101 gaaccggtca ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa
     2161 cgtctgttgg gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc
     2221 aaacgcttgt tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag
     2281 aacacagcca acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc
     2341 gatgcagaac cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa
     2401 tacgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
     2461 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
     2521 aaaaacgcat ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat
     2581 ccacgttgtg gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc
     2641 cccctttaca ccttaaggct gttcccacgc acagaacaaa atgtttatgc ctggttgagt
     2701 aaataaccat gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt
     2761 cattgttctc ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga
     2821 attttctctt tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat
     2881 ggtcagaacg accagttcac accagccacc gcgttccacg gggactccac accggcacca
     2941 tggctatacc ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct
     3001 gcctggtata ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact
     3061 ttcacccgat tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt
     3121 tccataccgt tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata
     3181 ctgcgggtat cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt
     3241 gacctaagct ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag
     3301 agacagcgca ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata
     3361 tcccgcataa gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa
     3421 atcactcttt tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa
     3481 aataaaatga ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac
     3541 aataatattg ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca
     3601 cttcttgaat aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt
     3661 cacgtaaaaa acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat
     3721 agcgagcgtt ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact
     3781 tacttaatac cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc
     3841 attgttctgc tccacgccag cgatagcgga tatgaaccgc accacgaaag gtgcattgct
     3901 aggcgcaggc gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc
     3961 tgttggcgct ggtgttagtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa
     4021 aggtgcaaaa gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct
     4081 cgaaggggcg attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat
     4141 gaaataagga atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc
     4201 aatgacctgg gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac
     4261 ttgcgcagaa gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc
     4321 ggtaatgggt aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact
     4381 gagcgccaaa gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt
     4441 gatcacggca attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac
     4501 cgcgtcgatg aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag
     4561 gcccaccatc actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc
     4621 ccagacgctg gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt
     4681 actcacccca tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt
     4741 atggatccag ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt
     4801 ctcctgagta cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc
     4861 attctcaata cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat
     4921 agcctgtgcc tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt
     4981 attcagggca aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga
     5041 ttcataggct aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga
     5101 gtcaatgtat gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc
     5161 gctatagcct ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt
     5221 gtagtcgcgg gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc
     5281 accggttaac agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg
     5341 tccgttgctg tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga
     5401 acgctcctgt tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg
     5461 cataacaaaa agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg
     5521 caggacgggg gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc
     5581 gctcgttgcc tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg
     5641 ggttaatgaa tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt
     5701 tgattccccc tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc
     5761 tgcattggta actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc
     5821 cgtagctgaa ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac
     5881 gtaattttca gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat
     5941 atagaccgta ctattatcaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca
     6001 ggtactatta tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc
     6061 tgaacccgtt atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat
     6121 aaaaacatct gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatc
     6181 tttatggtag aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt
     6241 tgcttcctga ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc
     6301 agatgtattt ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg
     6361 acccgttgta taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc
     6421 atgagcattg tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt
     6481 ataatcaagc gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc
     6541 tgttccgtgg gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc
     6601 attacttcct gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct
     6661 tccgggagat gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt
     6721 atcatctccg tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt
     6781 gtcagtattc aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt
     6841 gacaatcgtg tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct
     6901 agctgttaat gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac
     6961 aatgacgcca ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt
     7021 ccattacccg ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt
     7081 gccacagcat gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc
     7141 gccagagcaa taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta
     7201 tccattcatt ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa
     7261 acggagggta aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata
     7321 tataaatgat aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac
     7381 tttggggtga ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc
     7441 catggtaatt atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta
     7501 tttgcgcaat aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag
     7561 ccgcatactg acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt
     7621 tctcttaaat cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat
     7681 cgtcacaagc acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt
     7741 gatcagcata aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact
     7801 attattcatg ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa
     7861 taatgaacaa tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact
     7921 atggccttac tgatcaattt tcactcaacc acaaccttca ccttcggttt agaaaggata
     7981 attttgacca ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg
     8041 atattatttt ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata
     8101 ctgatcgaaa gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg
     8161 tctgcgttta cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca
     8221 tgaagataag aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca
     8281 ttttcatgca gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc
     8341 tcggtgactt ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa
     8401 cactcgtcga caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga
     8461 aaaccttccg gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg
     8521 ctttgtgtaa gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt
     8581 gaaatatatc caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc
     8641 ccttcccgac catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc
     8701 cgtcgcagga tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca
     8761 gcggcgctgg cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg
     8821 tagaagctaa agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga
     8881 accttctcgg tatagggata atgtccatta agcgtcatca catcagccgt tggtccaata
     8941 atgtcatccg ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg
     9001 cgaatatgtt gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctctgatag
     9061 agcaggatag gtaatctatt aacggtgtcg attgccggag ccaacagtaa attactgtcc
     9121 gggacaacag gaatacgaga cagcaggacc cgattcccag gcagggatgt acaccagact
     9181 attccctgtt tgagaatgat gattgtgcgc aggtgaggtt tcagagctgc ttcagtgcca
     9241 agctgatatt gctcctcgag gtcgcattcc ttcccggcaa tgttaaccgc tgtttgagtc
     9301 gcctgtgacg cttcttcaag tatatgattg atattgttgg cggcatatct tgctcccgcc
     9361 aggtactctg cttttgcgga ataccagagc tggatgttca aaatagcgac accggtaata
     9421 aacaagcata tcgcaacgag gatgggtatg agtgtattgc gcatttgctt gtcccggcca
     9481 aagggtttaa taca
//
LOCUS       DAZPAB010000150         9479 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZPAB010000150 DAZPAB010000000
VERSION     DAZPAB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.150"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="ACHUPG_003111"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="ACHUPG_003111"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR0862624.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="ACHUPG_003112"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="ACHUPG_003112"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR0862625.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="ACHUPG_003113"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="ACHUPG_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFR0862626.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="ACHUPG_003114"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="ACHUPG_003114"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR0862627.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="ACHUPG_003115"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="ACHUPG_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR0862628.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="ACHUPG_003116"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="ACHUPG_003116"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR0862629.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="ACHUPG_003117"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="ACHUPG_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFR0862630.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..9206)
                     /locus_tag="ACHUPG_003118"
     CDS             complement(8064..9206)
                     /locus_tag="ACHUPG_003118"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFR0862631.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9215..>9479)
                     /locus_tag="ACHUPG_003119"
     CDS             complement(9215..>9479)
                     /locus_tag="ACHUPG_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 C-terminal
                     domain-containing protein"
                     /protein_id="HFR0862632.1"
                     /translation="SEYYTRFINDDTVFIHYTGITKPWHDWANYASADYFRNIYNISP
                     WRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNVMKG"
BASE COUNT         2631 a         2189 c         2075 g         2584 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctggc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa
     9241 ttgctgtaaa aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt
     9301 tgtgctcatg ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat
     9361 aaatattacg aaaataatct gcagaggcgt agttcgccca atcatgccat ggcttagtta
     9421 taccagtata atgtatgaag acagtgtcat cattaataaa ccgggtgtaa tattcacta
//
LOCUS       DAZPAB010000151         9440 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZPAB010000151 DAZPAB010000000
VERSION     DAZPAB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.151"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..1154
                     /locus_tag="ACHUPG_003120"
     CDS             <1..1154
                     /locus_tag="ACHUPG_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862633.1"
                     /translation="EDMCERMKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKY
                     SAKDVGPDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNV
                     PKNNKPKTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACE
                     AHSHRSVALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFN
                     QSDISVRNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVM
                     GCKSAKDRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKD
                     LFDIRVLHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            1833..2051
                     /locus_tag="ACHUPG_003121"
     CDS             1833..2051
                     /locus_tag="ACHUPG_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFR0862634.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2269..2511
                     /gene="yiiF"
                     /locus_tag="ACHUPG_003122"
     CDS             2269..2511
                     /gene="yiiF"
                     /locus_tag="ACHUPG_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR0862635.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="ACHUPG_003123"
     CDS             complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="ACHUPG_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR0862636.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="ACHUPG_003124"
     CDS             complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="ACHUPG_003124"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR0862637.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="ACHUPG_003125"
     CDS             complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="ACHUPG_003125"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR0862638.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="ACHUPG_003126"
     CDS             complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="ACHUPG_003126"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7777..7779),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR0862639.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            8558..9391
                     /gene="fdhD"
                     /locus_tag="ACHUPG_003127"
     CDS             8558..9391
                     /gene="fdhD"
                     /locus_tag="ACHUPG_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR0862640.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHVAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2311 a         2314 c         2446 g         2369 t
ORIGIN      
        1 ttgaggatat gtgtgaaaga atgaaaggtg gcatctctat gtttaaccac accacaaaaa
       61 cagctgataa tatagagcat aatttgcagc taagttttct cgtgggttca cgtccgtcga
      121 tcaccaataa atactcagca aaagatgtag gtccagatat acttgcattg cccgtcaacg
      181 tatatcatgc ggattttaaa attaaaatca ataatgcagt ggagaaggct tcatcaattg
      241 tgttatgtgc tgtgcaaaaa aatctccatg cagcaaggaa ggttcttagc gggataatag
      301 gaaatgtgcc aaaaaataat aaaccaaaaa caattatttt tgatttgcgt tttcttagtg
      361 caattaagca gaaagttttt ttgggcagtg aaaaaccgtt gtttgtaaag cataccgcaa
      421 tgatgaggga ggcatgtaag gaattaccgc agtcagtgga atatgttcct ttagcctgtg
      481 aagcgcatag ccatcgcagc gtcgcccttg ccaaaaccgt ggctgcgccc atcatggcga
      541 caggtgtggg cttggcatta atgtatgcaa catcatcagg accgtattat gcgcaaagtc
      601 tttctggttc gccggatatc gagatgataa aaaatcgcat ggctgaattg tttaaccagt
      661 cggatatttc tgtgcgcaac aaatatagcc cggcttttga taaatggaat gaactggtag
      721 ataaattaaa tcacgaaaga aatacagttc cgtttgcgtg tttgaccacc attttagcga
      781 cggcgataaa tgaaactctt gagggggata cgaatgttgc cgttgtgatg gggtgtaaat
      841 ctgcgaaaga tcgcacgata tccattgtcc ttggcaactc gatgttgcaa acactccttg
      901 aaaaacgcct cgccgatagt ggagaaatag aaaatctttt tgatcaacag ggatatttta
      961 gttgcgattc attaacaccc gaagaattaa tgatgttaaa agaccttttt gatattcgag
     1021 ttcttcatgt ctctaacaaa ttcaatgttg gattgcaagg taatatcaac actgatgttt
     1081 tacaagattc gttttttaaa aatgtcgatt tcattcatga atataactcg tataccgtag
     1141 ggatgggagt ctgacgagta aaactcatta ggattatcag aattcggaca atgcattgca
     1201 aaaatgacat acaaagagag atgtgtgcag gtacttagcc gcgaggggca tggcgaagcc
     1261 atacagaaag taccagtatt cagaactcca gtgaaagcgg catagtggcc gcctgctcag
     1321 gagttttgct atgacccgta atcgtgctga acgtcgccac cctatgatgc gattaaaaaa
     1381 actcatcgtc gcgctgatga ttgacgtcaa tgctatcgga tgatgccggg ggtgttccgg
     1441 aaggaattcc ttgtttcccg aactgttacc aacaaaatta taacgatgag caagtaaaga
     1501 ccggttggtg gcagtactga ccggtctgtg atgaaagatt aatttcacct taaatcctgg
     1561 tgctgttagg atgctgacgt ttttcggcta caccattcag attatgtaaa taaatatccc
     1621 tgaatataat atattcatag cacccataac tcatcctggg cgataactaa accatttttg
     1681 agtaataaat gaaacaactg caacgctctt attatttctg gaaaacgcct cgcaaaaaca
     1741 aaatgacatg caaaaatccg cattttttta aatgcttttc cggcgtaatt ttcgcacata
     1801 cattaatgag taatatatgt aaggtggtgg taatggcgat gaatacggtt tttcttcatt
     1861 tatcagaaga ggcaattaaa cggctgaaca agcttcgggg ctggcgtaag gtttcacgat
     1921 ctgcaatttt acgcgaagcg gttgagcaat atctggagcg gcagcaattt ccggtgcgta
     1981 aagcaaaggg aggcaggcaa aagggggagg tagttggtgt tgatgatcag tgtaaggagc
     2041 ataaggaatg aaaaaaattt ttcattcttg aatataaaaa acagatgccc ttattctggt
     2101 attaatacaa ggctgtttta cttgaactta taataactgc aactgttaca tcgtatctgg
     2161 aaaacgcctc gcaaaaataa aaaatgatgc gtaaatgagc tttctttaaa gattcggcaa
     2221 gggtactttt ctctccatag attcaaaaat gatattggcg agatatttat gaactctctg
     2281 gcaggtatag atatgggcag aattttactc gatttatcga atgaggtgat taagcaactt
     2341 gatgatctcg aggtgcagcg taatcttcct cgtgcggacc tattaaggga agcggtagat
     2401 caatacctga taaatcaatc gcaaacagca agaaccagtg ttcctggcat ctggcaaggg
     2461 tgtgaggaag atggtgtcga atatcagcgt aagctgcgcg aggaatggta atgggaaaaa
     2521 atgatgtcaa tcagattgct gacaacgtgc gcgttgttca tgccggatgc ggcgtgaacg
     2581 ccttatccgg cctacaaagt catgcaaatt caatatattg aagtttattg gtaggcctga
     2641 taagcgcagc gcatcaggca atcatacgct tgtcatcagt gacataccca taaccttatc
     2701 gatgtgttgg agggaagaac gaggcaaaca gacaattgcg gcatgatctt gaatgggtaa
     2761 cgcggaatac gagagatggt gtcgtcacac cctatgagct gtaatccggg cgacctgcgc
     2821 cacccggaaa acgtcagaga ttactctcct tcacccggaa acaggaacgg gttaatggaa
     2881 ctacgggcgt agccttcttg ctccattcgc gcgtccagta ccagagaggc gaggtcatct
     2941 gccacggctt caactttcgg ttctttttcc tgataaagaa tcttcaggta agtgccgcag
     3001 tcatcgcagc tttcggcttt aatcgcggcc tgttcgtcat ccagcgacca gtaatgcagt
     3061 ttgccgctct gctcgcagtt gctgcatttt acgcgcacta cgtgccattc ggtttcacac
     3121 aggttgcagt gcaggtaacg cagaccctga gtggtgccaa tttgcaccat gctggacacc
     3181 ggcatagagc cacataccgg gcaatattga cgttgttcgc cgtattcagc gcgggctttg
     3241 ccggggatca gattggccat ctgcgcccag tagagcgaca gcgcagccca gataaacggc
     3301 gctttatcgc tgctgacgga cgagaaatca gaggcaaaca gcgcgctggc catatcttcc
     3361 agctcctgag tcgatgcctt ctccagattc tcaatcactg ccagcgccgg gccgctcatt
     3421 tcaggtttca gctcagcaat tagcgccatc agcagctttt gccagtgctt atcacgcggc
     3481 agaacgtgaa tatccagcgg gggcttgccc tgtgcgctgg cttctttaat gcgcgcggtc
     3541 agatccatct ccagcggatg gtcgtacagc accactttct gggcgtgggc gataagcgca
     3601 gcaaagcgca ggtaatcacc cagcggatta ttttctgcca gctcgcgcag acgctcggcg
     3661 cggcggttgt ataaattctt gagccgaggg aacaataacg gcggaatcat atccgccgta
     3721 cgtttctcgc tcgaacccag ctcatcttgc gggattatgc gaatactcat tcagctttct
     3781 tttccgttgt cttgcggacc tcacggtacc agcgcgggtg atgtttcttc gcccaggcgc
     3841 tggtaaccca tccttccacc atggcggtaa tcgtgccttt cacccaaagg gcggcgtaga
     3901 tatgcaccat gataaccaca attaacgcca ctgcggcaaa tgaatgcagc attaacgcga
     3961 atcggatcac cgggattgag aaagcaggcg caaaataagg acgccagata atcacgccgc
     4021 tcaccagcag cagaaccagg aaaataatcg ccgcccagaa aacgcatttc tgaccgaagt
     4081 tataacgccc ggtgtcacct acttcctcgt tgacgacgat cttacgaata ttcttcgccc
     4141 aaaagatatc atcccgattg attaggtttt gatgccagta acggaaaaac atgatgatga
     4201 acgaggcaaa cataaccacg ccgacaaacg ggtgcagaat tcgcgccagc tgcggtgtgc
     4261 ccatgatttg catcaaccag ttgaaggacg ggaacaaaaa gcccagcccg ctcaccgccg
     4321 ccaggatgaa gcagaaggcg gtgatccagt ggttgatacg ttccggcgcg gtgtagcgca
     4381 cgatggtgtc acgtcgtttc atttgcgctc ctcgtctttc tcttcgtgca gattattctc
     4441 ttcctcatcc gcacggttcg gaccgacacc cacgtagtgg aagatactgg ccgcaaaggt
     4501 agccgcaaag ccaacagccg cgagcggttt ccagatgcct ttccagaatt tcacggtttc
     4561 gctgatttcc gggttctccg gcaagccatg atacagattt ggcttgtcag catggtgcag
     4621 cacgtacatg acgtgtgtac caccgacgcc tgccggatcg tacagacccg cattgtcgta
     4681 accgcgggtt ttcagctcag caacgcgctc gctcgccagc gttttcatcg actctttcgt
     4741 gccgaagtga atcgcgcccg ttgggcaggt cttcacgcag gctggttctt gcccaaccac
     4801 cacgcggtca acgcacagcg tacatttgta gacgcggttg tcttccgggt tgaggcgcgg
     4861 aatgtcgaac ggacagcccg caatgcaata accgcagcca atgcactgct cggactggaa
     4921 gtcgacgata ccgttggcat actgaataat tgcaccttcc gccgggcacg ctttcaggca
     4981 gcctggatcg gaacagtgca tacagccgtc tttgcggatc agccattcca gtttgtcgtt
     5041 ctgctccact tccgagaagc gcattaccgt ccacgatttg gcgcttaaat cattggggtt
     5101 gtcgtacacc ccaatgttat tgccgacggt atcgcgaatg tcgttccact ctgaacacgc
     5161 cacctgacag gctttacagc cgatacaggt ggtaacgtcg atgagtttcg ccacttcttc
     5221 ctggaagtcc cgcgcctgag gcgcgggggt cagaccgtta gtcgcggaac gacgaatgat
     5281 atcttgagat tgataagcca tatgtcgtct ccgttacacc ttttccacat tcacaaggaa
     5341 ggacttaaac tccggcgtct gcgtgttcgc atcaccaacg aatggcgtta aagtattggc
     5401 aataaagcct tttttagcta cgccttcata gccccagtga atcggaatac cgatggtatc
     5461 gatatctttg ccgttcgctt tcagcgtgcg aatacgtttg gtcaccaccg ctttggcttt
     5521 gatatagcca cggttggagg agactttcac ggtatcgccc tgggcaatgc caagtttatt
     5581 cgccagcgac tccccgattt ccacaaactg ctctggctgc aaaatcgcgt tcaacagcgc
     5641 gtgtttggtc cagtagtgga agtgctcggt cagacgatag gtggttccga catacgggaa
     5701 cttatcggct ttacccaatg cttcggcgtc gtctttaaag atacgtgcag ctgggttcga
     5761 gataacgttt ggatgcagcg ggttagttcc cagcggcgtt tcaaacggct cgtagtgttc
     5821 cgggaacgga ccttccgcca tcttatcgag ggcaaacaga cgccccatgc cttcctgctg
     5881 catgataaac ggcccgacgc cgctacccgg aggcgctgcg ctgtagtccg gaatatccca
     5941 gccggtccac ttagtgccgt cccatttcag caactgacgc ttcggatccc acgggttacc
     6001 ctgcggatct gcggaggcgc ggttatacag aatgcggcgg ttaagcggcc atgcccatgc
     6061 ccagcccagc gtgttaccga ggccagacgg atcggcgtta tcacgacgcg ccatctggtt
     6121 gccttccggc gtccagctac cggcgaaaat ccagcagcca caggaggttg taccgtcatc
     6181 gcgcaattgg gcgaacgagc taagttgttg gcctttcttg acgataaccg caccggttgc
     6241 cggatcggta atatcggcca gcgccttacc gttgctctcc atcgctactt cttccgactt
     6301 cggctcatgc ggaatggcgt agttccaggt catgttcagc acctgatccg ggttcgcgcc
     6361 accctgttcg gcatacatct tgcgcaagcg caggaagata ccggagagga tctcaccgtc
     6421 agtcagcgca atccccgggg cgtccgcacc tttccagtgc cactgcaacc agcgacctga
     6481 gttaacgatt gaaccgttct cttctgcgaa gcaggtcgat ggcagacgga acacttcggt
     6541 ctggatcttc gacgagtcaa cttcgttcag ctcaccgtgg ttctgccaga agttagaggt
     6601 ttcagtgttc agtgggtcga tggtgacgag gaacttcagt ttcgacagac aaccgatcac
     6661 tttgtttttg ttcgggaatg aggcaacagg gttaaagccc tggcagatat agccattgac
     6721 cttgccctct ttcatcatct cgaagtattg caggacgtcg tagcctttat cccacttcgg
     6781 caaccagtca aagccccagc tattttccgc cgtcgcttta tcaccaaaga aggccttcat
     6841 catagagacg aagaatttcg ggtagttgcc ccagtagttt acctggcctt ccagcagcgg
     6901 ttttggcgtg ttggcggtaa ggtaggtttg cagatcggtc tgcttctcgc ttggcagcgt
     6961 catgtaacct ggcagactct gcgacagtag ccccaggtcc gtcagcccct gaatattgga
     7021 gtgaccgcgc agggcgttaa cgccgccgcc ggccatcccc atattgccga gcagcagctg
     7081 gatcatcgcc atcgtacgaa tgttttgcgc accaacggag tgttgcgtcc agccgagggc
     7141 atacaggaac gaggcagttt tatcgtgagc actggtttct gcgatgtatt cgcagacttt
     7201 caggaacgcg tcttttggcg taccacagat gttttcaacc acatctggcg tgtagcggga
     7261 aacgtgctgt ttcagcaagt tccacacgca gcgcggatgt tgcagcgtgg tatcgcgttt
     7321 ggcgaagccg ttttcgtcca gttcataagt ccaggaggat ttatcgtact tgcgtttttc
     7381 cgcgtcgtag ccggtgaaca ggccatcttc aaagccgtaa tcctcacgca cgatcaggct
     7441 ggcgttggta taggcttcgg tgtattcgtg gttgaatttt tcattgttca gcaggtacag
     7501 caatacgcct gacaggaaag caatgtcagt accggaacga ataggggcat agtagtcagc
     7561 taccgccgcc gtacgcgtaa agcgaggatc gatcacaatc agcttcgcgc cgttgtgaat
     7621 tttggcttcc atcgcccagc ggaacccgac cgggtgagct tcagcggcgt taccgcccat
     7681 caccacgacg aggttggcgt tcttgatgtc gacccagtgg ttggtcatcg caccgcgacc
     7741 aaatgttgga gcaagacttg ctaccgttgg tccgtgtcag acacgcgcct ggttgtcgac
     7801 cgcgagcata cccagcgcgc gggagaattt ttgcgttaaa tagccggttt cgttactcga
     7861 cgcggaagca cacagcatcc cggtggagag ccagcggtta acagtcacgc cttcggcgtt
     7921 ttgcgcaatg tagttagcat cgcggtcttc tttcatcagt ttggcgatgc gatcaaacgc
     7981 ctcttcccaa ctgatttgct gccatttatc agaacctggc gcacggtatt ccggaaactt
     8041 cagacggctt tcggagtgga tgaaatccac caggccagcg cctttcggac aaagtgcacc
     8101 gcggttgacc gggtgatccg gatcgccttc gatatggaag atagatgctt tggcgttttt
     8161 tgctccgtca ccgaggctgt acatcaacag cccacagcct acggaacaat aggtgcaggt
     8221 attacgggtt tcgcgggtgc gcagcagttt atactgccgg gtttccgcga gtgctacgct
     8281 gggtgcaaaa cccagtgccg ctgccgtggt gcctgccata ccgccagcgc agatcttaaa
     8341 gaactgcctt ctgctgacct gcatggattg ctccttgttt cgacattgtc acgtcccatt
     8401 tacattcgct tgctgcgtgt gcagggagtg ggagttattt ttctttgcgg aaggggccgc
     8461 aaaggtccag aattggctca atttccctcc atctaggaag ggtttgtaac agaataccat
     8521 aatgttggtg tgtatgttct tatctggtta agagaaagtg aaaaaaacac agcgaaaaga
     8581 aatcgaaaat gtgacaaata tcacaggtgt tcgtcaaatt gagttatggc ggcgtgatga
     8641 tttacaacac ccacggttag atgaggtcgc ggaagaagtt cccgttgcgc tggtctacaa
     8701 cggcatttcg catgtggtga tgatggcgtc gcccaaagac cttgagtact ttgcgctcgg
     8761 tttttcgctt tccgagggga taatagaaag tccgcgcgat atcttcggca tggatgtcgt
     8821 tccttcctgt aatggtcttg aagtacaaat tgagctttcc agccgccgct ttatggggtt
     8881 gaaggagcgc cgccgggcgc tggcgggacg tacgggctgt ggcgtatgcg gcgtggagca
     8941 acttaatgac atcggaaaac cggtgcagcc gctaccgttc acccagacgt ttgatctcaa
     9001 caaactggat gatgcattac gtcatctcaa cgattttcag ccagtggggc gactgactgg
     9061 ttgtactcac gtcgctgcct ggatgttgcc atctggcgaa ctggtcggcg ggcatgaaga
     9121 cgtgggtcgc catgtggcgc tggacaaact gttaggccgc cggtcacaag aaggggaaag
     9181 ctggcagcaa ggtgcggtac tggtttccag tcgtgccagt tatgaaatgg tgcaaaagtc
     9241 ggcgatgtgc ggcgtagaga ttttgtttgc ggtgtctgcc gcgaccacgc ttgctgtaga
     9301 agtggctgag cgctgtaatc tgacactggt aggtttttgt aaaccgggtc gggcaacggt
     9361 ttatacccat ccgcagcgtt tgagcaatta attaaaataa gtcctgcgat atatattaaa
     9421 ttgcaggact tattcatttc
//
LOCUS       DAZPAB010000152         9389 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZPAB010000152 DAZPAB010000000
VERSION     DAZPAB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.152"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(38..940)
                     /locus_tag="ACHUPG_003128"
     CDS             complement(38..940)
                     /locus_tag="ACHUPG_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFR0862641.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            complement(1078..2319)
                     /gene="yihS"
                     /locus_tag="ACHUPG_003129"
     CDS             complement(1078..2319)
                     /gene="yihS"
                     /locus_tag="ACHUPG_003129"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFR0862642.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            complement(2336..3214)
                     /gene="yihT"
                     /locus_tag="ACHUPG_003130"
     CDS             complement(2336..3214)
                     /gene="yihT"
                     /locus_tag="ACHUPG_003130"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFR0862643.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(3238..4134)
                     /gene="yihU"
                     /locus_tag="ACHUPG_003131"
     CDS             complement(3238..4134)
                     /gene="yihU"
                     /locus_tag="ACHUPG_003131"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR0862644.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            4302..5198
                     /gene="yihV"
                     /locus_tag="ACHUPG_003132"
     CDS             4302..5198
                     /gene="yihV"
                     /locus_tag="ACHUPG_003132"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFR0862645.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5232..6017
                     /locus_tag="ACHUPG_003133"
     CDS             5232..6017
                     /locus_tag="ACHUPG_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR0862646.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            6116..6715
                     /gene="yihX"
                     /locus_tag="ACHUPG_003134"
     CDS             6116..6715
                     /gene="yihX"
                     /locus_tag="ACHUPG_003134"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR0862647.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            6709..7581
                     /locus_tag="ACHUPG_003135"
     CDS             6709..7581
                     /locus_tag="ACHUPG_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR0862648.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            7578..8015
                     /gene="dtd"
                     /locus_tag="ACHUPG_003136"
     CDS             7578..8015
                     /gene="dtd"
                     /locus_tag="ACHUPG_003136"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR0862649.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            8060..9001
                     /gene="fabY"
                     /locus_tag="ACHUPG_003137"
                     /gene_synonym="yiiD"
     CDS             8060..9001
                     /gene="fabY"
                     /locus_tag="ACHUPG_003137"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR0862650.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2197 a         2355 c         2497 g         2340 t
ORIGIN      
        1 atgttcttca atgattttga ctgctaatca atacaagtta ttttctctgt ccgtaaatat
       61 taaaaaacag gcggtgcggt tttcctggtt ccagtaataa taagtcgata cctgaattaa
      121 aggcgttagg cgggcaactc ataggttcaa ctgccagtcc ctgacgctgt aatttttcac
      181 cgctataaac ctgcacccag ggttgatcgg aacaaaggct taccgaaagc gcctgctgtg
      241 gatgggtaat ggtcatttcc cacaaatcat tagcggtttt aaaggtgtgg tcgatttttg
      301 tcgcggctat ttttttcgcc tgcgtgaaat ttaaatccag ctcatcaacg tggtgaagcg
      361 ttgtcggatt tacgtgttca tcgacggcat aaacctggtt tgccggaagc tggaacagat
      421 attcatcaac agatgttaag ttgcaggtta aataaggatg aatacccacg ccataaggcg
      481 ctgcgacagt gccgatattc tggcttgcga tctcaacgga aagacccgtg tgtgcgttga
      541 gcgaataaac aacttgtgaa gccagcataa acggatagcc gtaactgggc ggcagaaaag
      601 ctgtcagggt aacgctggtg gtggttaatt cactaatttg ccagtctcgc caggcaagca
      661 gcccgtgaat agcggctttt gaactatgtt cattaatggg taactgatat tcctgcccct
      721 gataacggta acagccgttg gcgatccggt tcggccacgg aataagcact ttacccagat
      781 gcgccagcgg catctcttcg ggtttgtgcg ggatgactaa atgacaaccc tgaaaggtta
      841 attcagccag accagcaccg acggtcacaa tggtcgcgtg ataatccccc gcggcaagtg
      901 aaacggtctg tccactgcaa tgcatattgg tgatttgcat acaaggcacc ttaattaacg
      961 acatacagcc tcccgtgagg ctgatgtcaa acccaaaaac tgcaccggac ggtcccctcg
     1021 cccctttggg gagagggtta gggtgagggg aaaacccgca ccctattttc agatcagtta
     1081 tttcgcatta atatccagca gacccgctgc aaccgctgga gccatgccgg gcgctaacgg
     1141 gatacgcggg atcaccaggc aatgcagcag gtgataaata tcctgtttgc cgtcccagac
     1201 tttggtgatg accttattgt ccgcatccag ctcctgccac caggaaccat tttcatagtc
     1261 catcaggtac ttaatgcagt actcccacca tgtttgatac caggtttcat actggcgatc
     1321 gccagtgacg gtgtagagcg cgtagaccgt acccattgct tcgacgatag gccaacgtac
     1381 acgttcgcgg accaccggtt ttccttccca gtcaacggta taaacaatcc cgtccgctcc
     1441 atcgggtgcc caggcatcgc gcacggtggc gttaaacaga cctttggcat cttctagcag
     1501 ccatgctggt ggttgttcgc aacgggcttc cagggcagca tggatgtgca gcattaaacg
     1561 gccccattcg atccagtggc ctggtgtacc tccgaacgcg cggaagcgat gcgccgggtt
     1621 atctttgttg taatccggca gcggattcca ctgggtatcg aagtgttcgt taacgcgata
     1681 atgattattt ctggcgacgt cgtggataat cacggaagcc acgcgaatcg cgcgatccag
     1741 ccattttttg tcgtgagtga cgtcataaac aatcaagaaa gcttccaccg cgtgcatatt
     1801 ggcattgccg ccgcggtact cttcggtttt gctgaaggct tcgtcccagg actccaggca
     1861 catctgctct tcttcgctcc agaaatattt ctcgataatc tcaatggtgt aatcgagcag
     1921 cttgcgcgct tccgggtgac ccgttgtgac ggcgctggcg gcacccagca gagcaaagaa
     1981 gtgttgatag ccctgtttgg aggcatccac cacgccctcg tcattcacgc aggcatacca
     2041 gccgccatat tttttatcac gcagtgcgcc gttcatggct ttaataccgt gatcaaccaa
     2101 cgcgtaagcg ccaggtcgac ccatcgccgc agcaacggaa taaacgtgca acatacgagc
     2161 tgtgatccac agatgggtgc ccatctcttc tttgatttgc cctttattgc ctaaccagcc
     2221 aaaaccagtc ggcactacgg aatttttacc aaaatcaaag atgcggtcgg tttcctgttc
     2281 cagccaacgg ttgtggctta gggtgttaaa ccatttcatt ccttttatcc tcatcttagc
     2341 ggcgtttagc catcatttcg tcgacgatat cgccaagttg ttgtaatttc ggtgcgcaaa
     2401 cgtcacggag catcagctcg ttgtctggta aaccgacgac cgatgcccag actgcacggc
     2461 ctgccaggaa tcccgatgcg cccgccgtca ttgccacgcg tacggcacgc gggaacagtt
     2521 tttcgtcgac actggaagag aggatcaccc atggcatatt gatatggtca ttcagacgtt
     2581 gtgaagcaca gagaagctct tgttgcggac ctttgccata aagcggcatt tcaactttgt
     2641 agaggtcagc gccactgtcg cccagctctt tggcggcatc gatgatcgct tgttcgcgat
     2701 cgaatttatc gccacgacgc ggtgggcgga cgactggctc aatgatgctt accagaccgt
     2761 gtgagtggca cagttcgttg aactctttca ccatatccag acgttgctgc gcatcttcat
     2821 cactacgcca cagcaccagc agttttaagg ctttgccacc gtcttgtttg atctgtagcg
     2881 gattgatttt gcggtcaata accacgctat cgaccggaat accgttgcca ggaatgaact
     2941 catcggcggc gacaatcatg gcgcaacttt tggcaatcgc gttttgctca accacctggc
     3001 ggtagcagaa ttgttgatct accagaatcg ccgaggcata aggcgagagg gcctttgcag
     3061 cgttaacttt gaaatcagtt aaaacgctat cggctaccgg agcgggtgcc ccagccgcgg
     3121 caaacatcat gcgcatggct tcgcgctgat cgaccgccag catggcaaaa ccgcccgatg
     3181 cgcgcgtaat gtcgttgatg gtgtacttat tcattcattt attcctttat ccagtcgtta
     3241 catttttact ttggcagtca tcccggcact gacacggacc tgttccagaa tggcggacca
     3301 gtcctggcga ccgcgacccg ctgcgcgcgc ctggctataa acctcccgtg aggcggcccc
     3361 cagcggcatt ggcacatgca gctggttggc gacatcaagg gcgatgccaa gatccttatg
     3421 ggcaagatcg atcatgaagg cgggagaaag atctccgctg aggactttgt ttggccagga
     3481 agtggtgaag tgacctttac cggcggcggt accgctcatc actttgacgg caacatcgaa
     3541 gggaagattc agggcttcgc acaaaacggc ggcttctgcc gaaagcgcat tgagcgcgat
     3601 gctcatgtag ttgttgatga gcttaacgcg gatccccatg cccggaccgc cagcgttgat
     3661 caactcactg cccatcgcca tcaggatcgg tgtggcacgt tcaacttgtt cagcggtgcc
     3721 gccagccagc agtaacagag taccggtaat ggcatttgca gaagtacggc ctaccggaac
     3781 atccatcatg ctgaagcctt tggcttgcat atcggcaatc aatttatcgg tttgcagcgg
     3841 atggatggtg gacatatcaa tgaccagcgc atcggtagat aagccttcgc aaacgccgtt
     3901 ttcaccgaac aacacgttgc gcaccagatc gccattcggc aacatggtaa tgataaattc
     3961 ggcatcttta gcggcctgcg ccgggttggc ggcgggagtc gcacctttgt ctaccagatg
     4021 ccgcacagct tcggcgttca catcaaagac gcgaagttga tgcccttgct gcaataaatt
     4081 gctcgccatt ggcgaaccca tttgtcctaa accgataaac gcgattgctg ccataaccct
     4141 ctcctgaata cagtgatgtc actttttgtc atttatgaca tgctttgctt gtctgttttt
     4201 gatcgaattt gtaatttatc gtcaaaaaat tgacagccgt cactttttaa acaattggtg
     4261 aaattaaaat gaacgcatcc caaaatgttt aaggaatgac catgattcgt gttgcttgtg
     4321 taggtataac cgtgatggat cgcatctatt acgtggaagg gttaccgacg gagagcggta
     4381 aatacgtggc gagaaattat acggaagttg gtggcgggcc agcggcgacg gcagcggttg
     4441 cggcggcaag gctgggggcg caggtcgatt ttattggtcg cgtaggtgat gacgacgcgg
     4501 gcaacagcct gctggcagag ctggaatcct ggggcgttaa cacccgttac accaaacggt
     4561 ataaccaggc gaaatcttcg caatccgcca tcatggtgga taccaaaggc gagcggataa
     4621 tcattaacta ccccagcccg gatctgctgc ctgacgcaga gtggttggag gaaattgatt
     4681 tctctcagtg ggatgttgtg ctggcagatg tacgctggca cgacggcgct aaaaaagcct
     4741 tcaccctggc ccgtcaggcg ggtgtgatga ccgttctgga cggggacatt acgccgcagg
     4801 atatcagtga gctggtggca ttaagcgatc acgcggcctt ttcagaaccg ggtctggcgc
     4861 gcttaacggg cgtgaaagag atggccagtg cgctaaaaca ggcacaaacg ctcacaaatg
     4921 gtcatgtcta tgtgacccag ggtagcgcag gctgcgactg gctggaaaat ggtgggcgtc
     4981 agcatcaacc ggccttcaaa gttgatgtgg tagataccac aggtgcggga gatgtttttc
     5041 acggcgcttt ggcggtggcg ctggcaacaa gtggggattt agcggagtca gtccgcttcg
     5101 ccagcggtgt agcggcgtta aaatgcacac gtcccggtgg acgagccggg atccctgact
     5161 gtgatcaaac ccgatctttt ttgtcacttt ttgtataaaa tgccagggtg atggtttttc
     5221 gaggaatttt catgagcctt accgaactaa ccggtaaccc gcggcacgac caactcctca
     5281 tgctgatcgc cgagcgtggg tatatgaata ttgatgagct ggcaaatctg ctggatgtct
     5341 ccacgcagac ggtccgccgg gatattcgta aattaagcga gcaaggcctg attacgcgcc
     5401 atcacggtgg cgcgggtcgg gcttccagcg tcgttaatac ggcgttcgag cagcgtgagg
     5461 tttcgcaaac cgagaaaaaa aaagcgattg ccgaagcggt ggcagactat attcctgatg
     5521 gttcaacaat atttatcacc attggtacga ctgttgagca tgttgcccgg gcgttactta
     5581 accataatca tttgcggata atcaccaaca gcctgcgtgt ggcgcatatt ctttaccaca
     5641 acccgcgctt tgaagtgatg gtgcccggcg gtacgttgcg ctctcataat agcgggatca
     5701 ttggcccttc agcggcgtcc tttgtggctg attttcgtgc tgattatctg gtaacaagcg
     5761 ttggggcgat tgagagcgat ggcgcgttga tggagtttga tgtaaacgaa gctaacgtgg
     5821 tgaaaacgat gatggcgcac gcgagaaata ttctgctggt cgccgatcac actaagtatc
     5881 atgcttcggc ggcggttgaa attggtaacg tggcacaggt cactgcgctc tttaccgacg
     5941 agctgccgcc cgctgcgcta aaatcacgct tacaagacag ccaaattgaa atcattcttc
     6001 cccaggaaga cgcgtagatt tttgtgaccg ttaacctggc ttcatacctt gccacatagc
     6061 caaacccatc ctttcccgct acagttaatt tcttgtggcg cgaaaggagg caaaaatgct
     6121 ctatatcttt gatttaggta atgtgattgt cgatatcgac tttaaccgtg tgctgggagc
     6181 ctggagcgat ttaacgcgta ttccgctggc atcgcttaag aagagttttc atatggggga
     6241 ggcgtttcat cagcatgagc gtggggaaat tagcgacgaa gcgttcgcag aggcgctgtg
     6301 tcatgagatg gctctaccgc taagctacga gcagttctct cacggctggc aggcggtgtt
     6361 tgttgcgctg cgcccggaag tgatcgccat catgcataaa ctgcgtgagc aggggcatcg
     6421 cgtggtggtg ctttccaata ccaaccgcct gcataccacc ttctggccgg aagaataccc
     6481 ggaaattcgt gatgctgctg accatatcta tctgtcgcaa gatctgggga tgcgcaaacc
     6541 tgaagcacga atttaccagc atgttttgca ggcggaaggt ttttcaccca gcgatacggt
     6601 ctttttcgac gataacgccg ataatataga aggagctaat cagctaggca ttaccagtat
     6661 tctggtgaaa gataaaacca ccatcccgga ctatttcgcg aaggtgttat gctaaaaacc
     6721 attcaggaca aagccaggca ccgtacccgt ccactatggg cctggctaaa actactctgg
     6781 caacgtattg atgaggacaa catgacaacc ctggcaggta accttgccta tgtgtcgttg
     6841 ctctcattag tgccgctggt tgccgttgtt tttgcgcttt tcgccgcttt tcccatgttt
     6901 tccgacgtca gcattcagtt acgtcacttt atttttgcca actttctgcc tgctactggc
     6961 gatgttattc agcggtatat cgaacaattt gttgctaatt ccaacaagat gaccgccgtt
     7021 ggggcgtgcg ggctgatcgt caccgcgtta ttgttgatgt actccatcga tagcgcgttg
     7081 aataccatct ggcgcagtaa acgagcgcga ccaaaaattt actcgttcgc cgtgtactgg
     7141 atgattttaa cgctggggcc gctgctggca ggggccagtc tggcgatcag ttcctatttg
     7201 ctctccctgc gctgggcgag cgatctcaat actgtcatcg acaacgtgct gcgtattttt
     7261 ccgttgctgt tgtcgtggat ctccttctgg ttgctgtaca gcattgttcc taccatccgc
     7321 gtacctaacc gcgacgcgat tgtcggcgcg tttgtcgccg cactcctgtt cgaagcagga
     7381 aagaaaggtt tcgcgcttta tatcaccatg ttcccgtcat atcagctcat ttacggtgtg
     7441 ctggcggtga tccccattct ctttgtttgg gtctactgga catggtgtat cgtcttgctt
     7501 ggcgcggaaa ttactgtcac tctcggggaa taccgcaaac taaaacaagc agctgaacaa
     7561 gaagaagacg acgaaccatg attgcattaa ttcaacgcgt aacccgtgcc agcgtcaccg
     7621 tggagggaga agtgacgggc gaaattggcg cgggactttt ggtgttattg ggtgtcgaaa
     7681 aggatgacga cgaacagaaa gcaaaccgtc tgtgcgagcg tgtgctcggc taccgcatct
     7741 ttagcgatgc cgaaggcaag atgaatctca acgtgcaaca ggcgggcggc agtgtactgg
     7801 tggtttccca gtttaccctc gccgcagata ccgaacgggg gatgcgccca agtttctcca
     7861 aaggcgcatc accggatcgc gcagaggcgt tatatgacta tttcgtcgaa cgctgccgcc
     7921 agcaagagat gaacacgcaa acaggacgct tcgctgccga tatgcaggta tcgctggtca
     7981 atgatggccc cgtgacattc tggttgcagg tatgagccag cttccagggt tgtcacggga
     8041 aacaagagag agtatcgcta tgtatcacct tcaggttcca caaacagaag aagagttaga
     8101 gcgttactat cactttcgct gggaaatgtt gcgtaagccc ctgcatcaac caaaaggttc
     8161 ggaacgcgac gcgtgggatg cgatggcgca tcaccagatg gtcgtcgacg agcagggtaa
     8221 tctggtggcg gtaggccgat tgtatattaa tgccgacaat gaagcgtcca ttcgctttat
     8281 ggtcgttcac ccggacgtgc aggacaaagg gttaggaacg ctgatggcga tgaccctgga
     8341 gtcggtggcg cgtcaggaag gcgttaagcg cgtgacctgt agcgcccgtg aagacgcggt
     8401 ggagtttttc gccaagctgg ggtttgttaa tcagggagaa atcaccacgc caaccaccac
     8461 gccgattcgc cattttttga tgattaagcc cgtcgccact ctggatgata ttttgcatcg
     8521 cggcgactgg tgcgcgcagc tgcaacaggc gtggtacgaa catatcccgc ttagtgaaaa
     8581 aatgggcgtg cgtattcaac aatataccgg gcaaaaattt atcaccacca tgccggaaac
     8641 cggcaatcag aatccgcacc atacgctgtt tgccgggagt ttattctcgc tggcgacgct
     8701 caccggttgg gggcttatct ggctgatgct gcgtgaacgc cacctcggcg gaacgattat
     8761 tctggcggat gcgcatatcc gctacagcaa accgattagc ggtaaacccc atgcggtagc
     8821 cgaccttggt gccttaagcg gcgatctcga ccgtctggcg cgcggacgaa aagcacgggt
     8881 gcagatgcag gtcgaaatct ttggcgacga gacgccgggt gcagtgtttg aaggcacgta
     8941 tatcgttctg cccgcgaagc catttggccc gtatgaagag ggcgggaacg aagaagagta
     9001 gcttatggag aggggcggtg ctgcctctct cattcattcc ggtcatttac atcagtccct
     9061 gactattccg gttatattga ataagaactt tataaatttt tattcttcgt tggcaccata
     9121 cgtgtcttgc tacatcactg ttcaggagca tttaaatatt atttgtagag ctgagtggca
     9181 attattgcag ataatgctta actcatcata ttagttcagg tttgagcgat ttttgatagt
     9241 caaaaccccc tctggtgcca tacttttttc ataaatattt ttaaaatcta tagctcacaa
     9301 atattatgca aagataatgg gctggcatac attgtcagta tcattcattt actgctctgt
     9361 atgttatgaa tcattatgtt gtatgtttg
//
LOCUS       DAZPAB010000153         9381 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZPAB010000153 DAZPAB010000000
VERSION     DAZPAB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.153"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="ACHUPG_003138"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="ACHUPG_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR0862651.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="ACHUPG_003139"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="ACHUPG_003139"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR0862652.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="ACHUPG_003140"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="ACHUPG_003140"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR0862653.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2433..3296
                     /gene="pdxY"
                     /locus_tag="ACHUPG_003141"
     CDS             2433..3296
                     /gene="pdxY"
                     /locus_tag="ACHUPG_003141"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR0862654.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACHUPG_003142"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="ACHUPG_003142"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR0862655.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACHUPG_003143"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="ACHUPG_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR0862656.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6165..6800)
                     /gene="nth"
                     /locus_tag="ACHUPG_003144"
     CDS             complement(6165..6800)
                     /gene="nth"
                     /locus_tag="ACHUPG_003144"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR0862657.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6800..7495)
                     /gene="rsxE"
                     /locus_tag="ACHUPG_003145"
     CDS             complement(6800..7495)
                     /gene="rsxE"
                     /locus_tag="ACHUPG_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR0862658.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7499..8119)
                     /gene="rsxG"
                     /locus_tag="ACHUPG_003146"
     CDS             complement(7499..8119)
                     /gene="rsxG"
                     /locus_tag="ACHUPG_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR0862659.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8123..9181)
                     /gene="rsxD"
                     /locus_tag="ACHUPG_003147"
     CDS             complement(8123..9181)
                     /gene="rsxD"
                     /locus_tag="ACHUPG_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR0862660.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9182..>9381)
                     /locus_tag="ACHUPG_003148"
     CDS             complement(9182..>9381)
                     /locus_tag="ACHUPG_003148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862661.1"
                     /translation="PEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAV
                     AAAIARVQAKKAAQQKVVNED"
BASE COUNT         2394 a         2351 c         2385 g         2251 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tctttttttt acatcccata
     5581 ttaacggggg tgctttctcg cgttatgtct aaaaaacata cgcgcaattg ttatagtggg
     5641 aggggaaacg gcaggtaatg ttcactatct tgcactttca gacaagtcct ttgtaataat
     5701 ctgttacata taactgaggc ggggaattca accattccgg gaaatgttat ccagcgttaa
     5761 atttttcaaa aaacgtaacg gcagattttt tcactgcatt gagaggggga tgatggcttg
     5821 gcggcgatat gttctgctat ttagcgtttt tcctagtgag atagtccagt ttctgaaaaa
     5881 tagccagtgt aatgttttgc aggtcaatat ttgttgcgtg tgtaaattta aatcatttta
     5941 acctgattct tgcaatgttt ttacattgcc acagcatcgt gatcttgatc acgaatgaat
     6001 agaaaatttc gctattaaaa aaacaattaa cctgcatttt tggttatccg caaacggatt
     6061 acctgatgat aacgctaagc aatggcatca ttgacgttaa aaagatgttt taaaagagga
     6121 taaagaaagg ttatcaatgg ggtaatcggt gttacccctt ttcttcagat gtcaactttc
     6181 tctttgtatt cacaaagatc ttcaataata caagagccac agcggggctt gcgggcaatg
     6241 caggtataac gcccgtgcag gatcaaccaa tggtggcagt cgactttaaa ctctgccgga
     6301 accactttca gtagcttttc ttctacctgt tcgacgtttt tccccggcgc aaattgagta
     6361 cgattacaaa cgcggaaaat gtgcgtgtcg acagcaatag tcggccagcc gaatgcagtg
     6421 tttaatacga cgttggctgt tttacgacct acgccgggca gggcttcaag cgcagcacga
     6481 tcttccggaa cctcgccatt atgttgttcc agtaatatac ggcaggtttt gatgatattt
     6541 tctgctttgc tgttataaag gccaatcgtt ttgatatagg ttttcacccc ttcaacgcct
     6601 agttcaagca tcgctgcagg cgtattcgcc accgggtaga gtttcgccgt cgccttatta
     6661 acactgatat cggtcgcctg agcggaaagc agtacggcaa tcagcaattc aaaaggcgaa
     6721 ctgaaattaa gctcggtggt gggatgagga ttgttctcac gcaggcgagt gaggatctcc
     6781 agacgttttg ctttgttcat cagacattcc ctgtttcacc gtttggcaat gcacgttcgg
     6841 cagctgcttc agcacggcgc tttttcattc tttcatcaat caggtatttt cctgccagca
     6901 tcagtcccaa gccaataaat gcacctggtg gcagcatcgc cagcaggaaa ggggagtcgg
     6961 tgtggaaaat ctccacgcgt aatacttttg cccagctacc taacagcgca tctgcaccgt
     7021 caaacaacgt gccattgccg ataatttcgc gtagtgaacc cagcacgaac atggcgcagg
     7081 ttgcgcccgt accaattgaa aagccgtcca gtgccgaaag cgccggacct tttttggcgg
     7141 cgaaggcttc agcgcggccc acaacgatac agttagtgac aatcagcgga ataaaaatcc
     7201 ctaatgattg atacaggcca aaggcgtagg cgttgatcag catctgtaca gcgctgacca
     7261 ccgaggcgat gatcatcacg taaatgggaa tgcggatctc ggctggcgtc cagtgacgca
     7321 gcgtcgaaat ggtcaggttg gtcagcgtca gtaccagcgt agtcgcaagt cctaaaccca
     7381 gagcgttagt ggcagtggat gtgaccgcca atagcggaca aaggccgagc agttggacca
     7441 gtgcagagtt gtttttccac aacccctgaa caataacgtc tttaatttcg ctcacgcttt
     7501 attctccaca ggcaggaagt tgagaaagtt gtgccggtaa cgtctgagcg tacaatccgg
     7561 cgcgttttac cgcattaacc accgcgcggg gagtaatcgt cgcgccggtg aactggtcga
     7621 aatcaccacc atctttcttc accgcccagt gcgcatcatc cgcaccactg attttttttc
     7681 ccgcaaaatg ggtgagccag tcagaaaggc gcaattcgat tttatcgcca agccctggcg
     7741 tttcgtggtg ctctgtcacg cgcgtgccaa gtaccgtgcc attaaaatcg gctcccacca
     7801 gcagctgaat cgcaccggaa tagccatctg gcgcggttgc ttccagaacg gcggctaccg
     7861 gtttgtcatc ctgtttggcg atgtaaaccc gatgctcacc tttacctaac tctggcgcag
     7921 ttaccagata gcaactctgt gcgagcgcat tgttatagcg ttcggctggc agcacctgat
     7981 caaataacgt cttttgttgc agactggcct gttcagcaat tgtcgttttg gtcatctggt
     8041 tgatggccgc agttaaccct gtagaacccg ctgcaaatag tgccagtgta atgccgtgtt
     8101 ttcggatagt tttcagcatg gtttaccctt tgcgatggcc gtagacgcgc ggacgcgtgt
     8161 agtaatcgat cagaggaacc gtgatgttcg ccagcaggac ggcaaaagcc acgccgtcag
     8221 gatagccgcc gaaactgcgg atcaaccaga ctaataagcc cgccagcgcg ccgaacatca
     8281 gccgaccacg attggtcgta gaagcagtaa ctgggtcagt caaaataaag aatgcgccga
     8341 gcatagtcgc accagacagc agatgaattt gcggtgctgc cagtgtttct ggtgagaaca
     8401 accagcccaa cgttgcgcat aacgccagcg ttactaagaa gctgagggga atatgccagc
     8461 gaatcgcttt ctgccatagc aaccacacgc caccagccag ccaggcgaga tttacccatt
     8521 gccaaccagc gcccgccaga ataccgctgt agatcggata ttgcataatc tgttcaaccg
     8581 aatgacctgc gcggacagag gttttgaatg tatccagcgg tgtcgcctga ctaatgccat
     8641 caatacctaa gcgtagcgta ttcatatcac caccactggc ggtatgaccg ctgaaaataa
     8701 cctgtatggc gtcgataaaa ccagggatgt tgaccgcaat ttcatgtggc ggtaaccagc
     8761 tggtcatctg caccgggaag gagatcagta agaccacata accaatcatt gccggattaa
     8821 acgggttttg ccccagaccg ccatacaact gtttagcgat gatcaccgca aacaccgtac
     8881 ccagtacgac catccaccat ggcgctaggg ggggaatact taccgccagc aataagcctg
     8941 tcagcaatgc tgagttatct ttcaacgttg cggctaccga ctgcttgcgt agtttgagta
     9001 cgagagcttc ggctaacaga gcactgaccg acgccaacag gatctgaacg agagtacccc
     9061 aaccaaaaaa ccgcagttgc gctgcgattc ctggcacggc tgcgagcaac accaacagca
     9121 taatgcgcga tgtctggcgc tggttatggg tataagggga gctagctatt ctgaatacca
     9181 tttagtcctc gtttacaacc ttttgctggg cggctttttt ggcctgaacg cgggcaatag
     9241 ccgcggcaac tgcggctttg cgcggatcga cctgttcttc tggttccgca ttagcctgtt
     9301 gctgttccag cttgcgtgct ttggcgcggg caatagcggc ttcgacggca gctttgcgcg
     9361 gatcaacctg ttcttctggc t
//
LOCUS       DAZPAB010000154         9357 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZPAB010000154 DAZPAB010000000
VERSION     DAZPAB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.154"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="ACHUPG_003149"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="ACHUPG_003149"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR0862662.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="ACHUPG_003150"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="ACHUPG_003150"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFR0862663.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="ACHUPG_003151"
     CDS             3115..3399
                     /locus_tag="ACHUPG_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR0862664.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="ACHUPG_003152"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="ACHUPG_003152"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR0862665.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="ACHUPG_003153"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="ACHUPG_003153"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR0862666.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="ACHUPG_003154"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="ACHUPG_003154"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR0862667.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQSLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQSHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8449..9330
                     /gene="yddG"
                     /locus_tag="ACHUPG_003155"
     CDS             8449..9330
                     /gene="yddG"
                     /locus_tag="ACHUPG_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR0862668.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2226 a         2476 c         2386 g         2269 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg aggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ctataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggctctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcga ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8461 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8521 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8581 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8641 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct atctggcgct ttccttaggg
     8701 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8761 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8821 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8881 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8941 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9001 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9061 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9121 accttaggat ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9181 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9241 agcgccccgc tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg
     9301 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggca
//
LOCUS       DAZPAB010000155         9184 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZPAB010000155 DAZPAB010000000
VERSION     DAZPAB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.155"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..923
                     /locus_tag="ACHUPG_003156"
     CDS             <1..923
                     /locus_tag="ACHUPG_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0862669.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="ACHUPG_003157"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="ACHUPG_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR0862670.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACHUPG_003158"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="ACHUPG_003158"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR0862671.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSNSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="ACHUPG_003159"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="ACHUPG_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR0862672.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="ACHUPG_003160"
     CDS             4446..4805
                     /locus_tag="ACHUPG_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR0862673.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="ACHUPG_003161"
     CDS             complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="ACHUPG_003161"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR0862674.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6059..6249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6314..8158
                     /gene="btuB"
                     /locus_tag="ACHUPG_003162"
     CDS             6314..8158
                     /gene="btuB"
                     /locus_tag="ACHUPG_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR0862675.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8103..8960
                     /gene="murI"
                     /locus_tag="ACHUPG_003163"
     CDS             8103..8960
                     /gene="murI"
                     /locus_tag="ACHUPG_003163"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR0862676.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2102 a         2220 c         2481 g         2381 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcgcc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccatgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatttgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcatcgcgca gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc
     5581 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
     5641 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt
     5701 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
     5761 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga
     5821 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
     5881 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
     5941 gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg
     6001 tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct
     6061 gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga
     6121 cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga
     6181 agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt
     6241 cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg
     6301 tggatgcttt acaatgatta aaaaagcttc gctgctgacg gcgtgttccg tcacggcatt
     6361 ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga
     6421 acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg
     6481 ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca
     6541 aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt
     6601 gctggtgtta attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct
     6661 tagccagttc cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc
     6721 tgtttatggt tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc
     6781 cggaacggaa atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac
     6841 gcagcaacaa ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca
     6901 tggttatgat gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg
     6961 ttttttaagt aaaacgcttt atggggcgct ggagcataac tttactgatg cctggagcgg
     7021 ctttgttcgc ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg
     7081 ttcaccgttg ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta
     7141 taacggcgaa ctgattaaat cacaactcat taccagctat agccatagca aagattacaa
     7201 ctacgatccc cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata
     7261 caccgtccag tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga
     7321 ctggcagaaa cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg
     7381 taataccggc atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc
     7441 agcacgcagc gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg
     7501 ttgggaattc atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcact
     7561 aaatctgggg caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa
     7621 acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata
     7681 tcgtaacgat gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga
     7741 agggaaagcg cggattaagg gtgtcgaggc gacagccaat tttgataccg gaccactgac
     7801 gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt
     7861 acgccgtgct aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg
     7921 gggtattact tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta
     7981 tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac
     8041 ctctcacctg acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt
     8101 ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac
     8161 cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga
     8221 tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt
     8281 atggcgaaaa aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc
     8341 aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac
     8401 ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac
     8461 ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta
     8521 aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc
     8581 tgggctcggc agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc
     8641 tggatgcact aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg
     8701 ttgtattggg ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag
     8761 agggaacccg gctggtggat tctggcgcag cgattgctcg ccgaacggcc tggttgttag
     8821 aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga
     8881 cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa
     8941 aactggcagt tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt attttaaatt
     9001 tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc
     9061 aaacacgccg acgggtcagc ggggttctcc tgagaattcc ggcagagaaa gcaaaaataa
     9121 atgcttgact ctgtagcggg aaagcgtatt atgtacaccc cgcgcagctg agaaaaagcg
     9181 aagc
//
LOCUS       DAZPAB010000156         9029 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZPAB010000156 DAZPAB010000000
VERSION     DAZPAB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.156"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACHUPG_003164"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACHUPG_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR0862677.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACHUPG_003165"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACHUPG_003165"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR0862678.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACHUPG_003166"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACHUPG_003166"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR0862679.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACHUPG_003167"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACHUPG_003167"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR0862680.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVQ
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4466..5590
                     /gene="dprA"
                     /locus_tag="ACHUPG_003168"
     CDS             4466..5590
                     /gene="dprA"
                     /locus_tag="ACHUPG_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR0862681.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5562..6035
                     /gene="smg"
                     /locus_tag="ACHUPG_003169"
     CDS             5562..6035
                     /gene="smg"
                     /locus_tag="ACHUPG_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR0862682.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6064..6606
                     /locus_tag="ACHUPG_003170"
     CDS             6064..6606
                     /locus_tag="ACHUPG_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR0862683.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6611..7183
                     /gene="tsaC"
                     /locus_tag="ACHUPG_003171"
     CDS             6611..7183
                     /gene="tsaC"
                     /locus_tag="ACHUPG_003171"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR0862684.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7188..8006
                     /gene="aroE"
                     /locus_tag="ACHUPG_003172"
     CDS             7188..8006
                     /gene="aroE"
                     /locus_tag="ACHUPG_003172"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR0862685.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8003..8260
                     /locus_tag="ACHUPG_003173"
     CDS             8003..8260
                     /locus_tag="ACHUPG_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR0862686.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8236..8790)
                     /locus_tag="ACHUPG_003174"
     CDS             complement(8236..8790)
                     /locus_tag="ACHUPG_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR0862687.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2259 a         2191 c         2251 g         2328 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gtgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacaacgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgctgca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag
     4441 gcttgcttat ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag
     4501 catcagcagc ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc
     4561 gcatattgat gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt
     4621 tctttcattt ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca
     4681 tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta
     4741 ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt
     4801 agtggggagt cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac
     4861 tctggcgacg tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt
     4921 gcataaagcg gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa
     4981 taccattcat ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc
     5041 tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa
     5101 tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg
     5161 ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg
     5221 tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct
     5281 tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga
     5341 cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga
     5401 gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg
     5461 ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc
     5521 agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt
     5581 atttgtttga aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg
     5641 aacaggatct taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc
     5701 tggaaaaact tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc
     5761 ctctctccat gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg
     5821 ggtttctgct tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga
     5881 tagagcgagt gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga
     5941 tcctgatggt gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat
     6001 tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt
     6061 gttatggcaa aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg
     6121 gctgaactgg ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg
     6181 gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg
     6241 gaggggcagg tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt
     6301 atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg
     6361 gacgaaacag caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc
     6421 cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac
     6481 ttcaaaccaa tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa
     6541 accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg
     6601 gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct
     6661 caatgaaaaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc
     6721 tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg
     6781 gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt
     6841 gactgacgtg cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt
     6901 tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg
     6961 agtcaccgac catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc
     7021 taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca
     7081 atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga
     7141 aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg
     7201 ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc
     7261 agcaactgaa tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca
     7321 acacactgaa cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta
     7381 aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag
     7441 ttaataccct caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag
     7501 gcttgttaag cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta
     7561 tcggcgctgg tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg
     7621 tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca
     7681 ctggcagtat tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta
     7741 ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc
     7801 atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat
     7861 ggtgtgagca gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg
     7921 cggctcatgc ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc
     7981 aattgcagga ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg
     8041 ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg
     8101 cgcgatctct ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt
     8161 agcgagtttt cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga
     8221 acaaagtgaa gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca
     8281 tttcacgtaa ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg
     8341 gatctgtttg acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg
     8401 gaccagacta cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat
     8461 tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg
     8521 accaacagtg acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt
     8581 atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata
     8641 atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac
     8701 gtcaccaatc acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag
     8761 atcgcggtat gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat
     8821 gactttgggg gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt
     8881 gtgtgcgatt gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat
     8941 acttgtgcaa aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca
     9001 cttcacagga tggtcggaac aacgaagag
//
LOCUS       DAZPAB010000157         8891 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZPAB010000157 DAZPAB010000000
VERSION     DAZPAB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.157"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            5..2177
                     /gene="espL4"
                     /locus_tag="ACHUPG_003175"
                     /pseudo
     CDS             5..2177
                     /gene="espL4"
                     /locus_tag="ACHUPG_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(2146..3883)
                     /gene="aceK"
                     /locus_tag="ACHUPG_003176"
                     /pseudo
     CDS             complement(2146..3883)
                     /gene="aceK"
                     /locus_tag="ACHUPG_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(4067..5371)
                     /gene="aceA"
                     /locus_tag="ACHUPG_003177"
     CDS             complement(4067..5371)
                     /gene="aceA"
                     /locus_tag="ACHUPG_003177"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFR0862688.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(5401..7002)
                     /gene="aceB"
                     /locus_tag="ACHUPG_003178"
                     /pseudo
     CDS             complement(5401..7002)
                     /gene="aceB"
                     /locus_tag="ACHUPG_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(7271..8200)
                     /gene="metA"
                     /locus_tag="ACHUPG_003179"
     CDS             complement(7271..8200)
                     /gene="metA"
                     /locus_tag="ACHUPG_003179"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR0862689.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8357..8800
                     /locus_tag="ACHUPG_003180"
     CDS             8357..8800
                     /locus_tag="ACHUPG_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR0862690.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2316 a         2163 c         2159 g         2253 t
ORIGIN      
        1 tcgtattcct cgtagttctt tctctgcaaa tattaataat acagcccaga caaatgaaca
       61 ccaaaccctg agtgaattgt tttataaaga actcgaggat aaattttctg gcaaggagct
      121 ggcgacgcct ctattaaaaa gcttctcaga gaactgtaga caaaatgttc gtcatatctt
      181 tagcaacaag gattttgtca ttaaattttc cacgtccgtc ttacaagctg ataagaaaga
      241 aattacgatg attaataaaa acgaaaacac gacactcact caaaccattg ctacaatatt
      301 tgaagaatac ctaatggaaa ttttacctca acgctcagac actcttgata aacaagaatt
      361 aaaccaaaaa tcagatagaa aagaaaaaga attcccaaga attaaactta atggtcaatg
      421 ttatttcccg gggcgcccca aaaccgtata gtataccgac acattgctgc acaatatatt
      481 aatgatattt atcagaatgt tgattacaaa ccccatcaag atgattactc ttcagctgaa
      541 aaatttctca ctcacttcaa caaaaaatgc aaaaaccaga ctttggcgtt gatttccagc
      601 cgtcctgagg ggcgttgcgt tgctgcctgc ggtgatttcg ggctagttat gaaagcatat
      661 tttgacaaga tggaatcaaa tggcatcagt gttatggcag ccatattact ggtggataac
      721 catgctttga cggtccggct aagaataaag aacacaactg aaggatgtac ccattacgtg
      781 gtttcggttt atgatcctaa tgtaactaac gataaaataa gaattatgag cgaaagcaaa
      841 gaggatatta aacactattc tctgatggat tttatgaatg tagattatag cctcctgaaa
      901 tggtcaaatg atcatgttat taaccaatct gttgcaataa ttccagcact tccgaaagaa
      961 cagctattga tgttaaaagg aactgtggat gaaataaccc ctccattatc accagcaacg
     1021 atgaatttgc taatggcaat tggtcagaat caccaactta cgcaactgat gattcagctc
     1081 cagaaaatgc cagaactaca tagaacagaa atgttgactg cctataatag tattaacctc
     1141 cccggtttat atttggctat aaattatggc aatgcggata tcgttgagac tattttcaat
     1201 tcattgtcag aaacaggata tgaagggtta ctctcgaaaa aaaatctcat gcatattctg
     1261 gaggcaaaag ataaaaatgg tttttctgga ttatttttag cgatatcacg taaggataaa
     1321 aatgttgtaa cctcgattct gaacgtctta cctaaactgg ccgcaacaca tcatttagat
     1381 aatgaacagg tgtataaatt cctgagtgcc aaaaatagaa cgtccagcca tgttttatac
     1441 catgttatgg cgaatggtga tgccgacatg ctgaaaattt ttttggtcgc gttacctttg
     1501 ttaattcgca catgtcattt gactaaagaa caggtactcg atctcctgaa ggcaaaggat
     1561 ttttatggtt gcccaagact atacctggcg atgcaaaatg gacatagcga tatcgtgaaa
     1621 gttattctcg aagcattgcc ctgtctggcc caggaaatta acatttcagc ttccgatatt
     1681 gtagatcttc tgaccgctaa aagtcttgcg cgcgacacgg gtttgtttat ggccatgcag
     1741 cgcggacata tgaacgttat taatactatt tttaacgcat tacccactct ttttaatacg
     1801 tttaaattcg ataaaaaaat atgaagcccc tcctcctggc aaataattct aatgaatatc
     1861 ccggtttgtt ttcagcgata cagcataaac aacaaaacgt tgtagagacg gtttatcttg
     1921 ctttatctga ccatgcacgc ctgtttggat ttaccgctga agatattatg gatttttggc
     1981 aacacaaagc gccacaaaaa tactctgcct ttgagttggc ttttgaattt ggtcaccggg
     2041 ttattgctga attaatcctt aatacattaa ataagatggc tgaaagcttt ggctttacgg
     2101 ataaccctcg atacattgcg gagaaaaatt atatggaagc tttactcaaa aaagcatctc
     2161 cccataccgt acgctaaatc tttgcctgcg ccgatacgca taaacatctt ccacatgccc
     2221 ttcacgtatg cggttttgta gtgcgcgcca gtaatcagcg cggaacaggt cggcgtgcat
     2281 ctcttcaaac agcggaccaa tacgcgggtc ggcgcatagc cagtggcgaa actcttccgg
     2341 gaaaacatcg cccggcgaga cgctgtacca cggttcgctg gcaagttcgt cttccggata
     2401 gcgcggcggc gggatgtcgc ggaaattcac ttccgtcatg tagcaaattt catcgtaatc
     2461 ataaaaaacc acacgcccgt gacgggtgac accgaagttt ttaaacagca tgtcgccagg
     2521 gaaaatgtta gcagcggcaa gctggcgaat agcgttaccg tattcttcaa tggcgtcgcg
     2581 caactgctga ccttccactt gttccagcca gatattgagc ggcaccatcc gccgctcgat
     2641 tatagagatg gcgaattaca atctgctcac caagatcggt gattttttcc gctgcttcct
     2701 ggagcagtaa ttccattaat accggggaaa tatgccgctt ctccagcaca aagttttcaa
     2761 actcctgggt gtccgccatt cggcccacac ggtcgtgctc tttcaccagt tgatagcagg
     2821 cacgaacgtg agcggcagac atctctttct gcggcgcgaa cttgtctttg atgactttga
     2881 atacccgatc aaagcccggc agcgtaaaca ccaacatcac cattccacga atacccggcg
     2941 cttcaatgaa ctgctcatta cagccctgta gataaacgag atattcgcgg tagctttcgg
     3001 ttttggcgtg cttctggcag ccgatagcca tatacaattc agcagtggtt ttacctggca
     3061 gaatttcccg tagccactcg accagcgctg cgggcagcgg tgcataaacc ataaaataag
     3121 aacgcgcaaa gccaaaaaca atgctcgctt cggcggtcgt cgtcaggcag gtatcaataa
     3181 ataactcgcc gtcgtccgtc tggtggatcg gcagtaagaa gggcaacgtg ccggaaggcg
     3241 tgatcagttt gcccaccagc caggcggctt tattgcggta aaacagttcg ttcgccacct
     3301 gtaaatgact ttccgcgagg ttgtctgtcc ccagcgtttc cgtcagatgg cgaatgatgt
     3361 aatgaatgtc acggctttta ttctgccagc gcaggcgcag cggcaggtcg ctgataacgc
     3421 gcatcagtag agattcccag ccgtgatcgg ggtgaaagtc tttcgccagc gggcggggaa
     3481 tggtacgaaa gcggcgctct ggctgagagc taaagataaa aagccgctcg ggagtaagcg
     3541 agcggtggtc aaataaccga cagtacacgg agttaaaaaa gctctccgca atctcgaagc
     3601 gcgggtaatc cggcaacagc cgcgtgtaat gctctttaac gcgcagtaaa aatgccgcgt
     3661 ccgtactttg gccgttagta atgcagcgca gttgctccac gaccagacca acgtgatgat
     3721 cgtaaagatg gatacggttt ttcatcgcct gctggacagc atgccagtcg gcctgttcga
     3781 aacgctgctg cgccccggaa gtcacttcga ggaatcgacc atactgagcg tcgaagcctt
     3841 gcaaaatggt ttgagcaatc agtaattcca ggccacgcgg catttagcgc cctcatcagg
     3901 agcagagaat tgccggatgc ggcgtaaacg ccttatccag cctacgttcg gcaacggttg
     3961 taggcatgat aagacgcgca agcgtcgcat caggcatcgt gcaccgattg ccggatgcgg
     4021 cgtaaatgcc ttatccggcc tacagtcagc aacgtttgta ggttgcttag aactgcgatt
     4081 cttcagtgga gccagtcagc gcggtgactg aagacgtgcc gccctgaata atagtcgtca
     4141 ctttatcgaa gtaacctgta cccacttcct gctggtgaga tacgaaggta tagccatctt
     4201 tcgcggcggc aaattccggc tgctgcactt tctcaacata gtgcttcata ccttcgccct
     4261 gcgcataagc gtttgccagg tcgaacatgt taaaccacat gctgtggata cctgctaggg
     4321 tgatgaactg gaacttgtag cccatatccg acagctgctg ctggaagctg gcaatagttt
     4381 tgtcgtcgag gtttttctgc cagttgaacg acggcgagca gttataagcc agcagtttgc
     4441 ccggatattt cgcgtggata gcttgtgcaa agcgacgcgc cagttccaga tccggcgtgg
     4501 aggtttcaca ccagaccagg tcagcatatg gcgcatacgc caggccacgg ctgatcgctt
     4561 gctcaatgcc cgcatgagta cggaagaagc cttcactggt acgctcgccg gtaataaatt
     4621 cgctgtcata cgggtcgcaa tctgaggtga tcagatccgc cgcatcagca tcggtacgtg
     4681 caaccagcag ggttggaacg cccgtcacgt cagctgccag acgcgccgcg accagtttct
     4741 gaatagcttc ctgagttggc actaaaactt tgccgcccat gtgaccgcat ttcttcactg
     4801 acgccagctg atcttcgaag tgaactgccg cagcaccggc ttcaatcatc gctttcatca
     4861 gttcaaaggc attcaggaca ccgccaaaac cggcttccgc atcggcaacg atcggcagga
     4921 agtaatcgac atagcgcgga tcgcccggct caatgcccac ggaccattgg atctgatcgg
     4981 cacgacggaa ggtgttgttg atccgctcca ccacagctgg caccgagttt gccggataga
     5041 gcgactgatc cggatacatg ctggccgcca ggttagcgtc cgccgctacc tgccatcccg
     5101 acagatagac tgcttcaata cccgctttcg cctgttgcag cgcctgaccg ccagtcagtg
     5161 caccgaggtt gttgatgtag ccttttttcg actcaccgtg cagcagacgc cacattttcg
     5221 ctgcgcccag ttgcgccagc gtgcattcag gattgactga accgcgtaat ttcaccacat
     5281 cttccgcact gtatgggcga gtaatgcctt cccaacgcgg ttgagtccac tctttctgta
     5341 attcttcaat ttgttgtgta cgggttttca tgtgcagatg ctccatagtt atgtggtggt
     5401 ttacgctaac aggcggtagc ctggcagggt caggaaatca attaactcat cggaagtggt
     5461 gatctgttcc atcaagcgtg cggcatcgtc aaaacggccc tgggagaaac gttcttcgcc
     5521 cagttcgctg gcaatgactt tcatctcttc gcccagcatc tggcggaaca aggctttggt
     5581 caccggtttg ccattgctca acgttttttg atgatggatc cactgccaga tcgaggtacg
     5641 ggaaatttca gccgtcgccg catcttccat caggccataa atcggcacgc agccgttgcc
     5701 ggagatccac gcttcgatgt actgcacagc cacgcgaatg ttagcgcgca taccttcttc
     5761 ggtgcgttca ccatcacaag gtgccagcag ctgatcggca gtaatcggcg cgtcttgttc
     5821 gcgcaatact tcaagctgat ttttacggga gccgagaatg tcgttgaata ccgccattgc
     5881 cgtgtccgca aggcctgggt gagcgatcca tgtgccatcg tgaccgttat tggcttccag
     5941 cgctttatcc gcttttactt tgttgagcac ctggttatta cgctcttcat ctttgctcgg
     6001 aataaacgcg gccatgccgc ccatcgcaaa agcaccgcgt ttatggcagg ttttaatcag
     6061 caggcgtgag taagcattca ggaatggttt atccatcgtc acggcctgtc tgtctggcag
     6121 gacacgatcg ggatagtttt tcaacgtttt gatatagctg aagatgtaat cccagcgacc
     6181 gcagttcaga ccaacaatat ggtcgcgcag cgcgtgaagg atttcgtcca tctggaacac
     6241 ggcgggcagc gtttcaatca gcaacgtcgc cttgatggtg ccgcgcggca gattaaagcg
     6301 atcttctgca tagctgaaga cttcgctcca ccaggccgct tcctgctagg actgggtttt
     6361 cggcagatag aaatagggac cgctgccctt tgccaacagt gcctgatagt tgtggaagaa
     6421 atagagcgca aaatcaaaca ggctaccggg gattgcctca ccacgccagg tgacatgttt
     6481 ttccggcaag tgcagaccgc gtacccgaca aatcaaaacc gctggattgg gcttgagctg
     6541 ataaattttg cctgcttcat tggtatagct gatggtaccg ttaaccgcat cgcgcaggtt
     6601 aatttgcccg tcgatcactt tgttccagtc tggtgccagt gaatcttcga aatcggccat
     6661 aaagactttc acattggcgt tcagtgcgtt gatcaccatc ttgcgctcta ccgggccagt
     6721 tatctctacg cggcggtctt ctaagtccgc aggaatcccg cgaattttcc aatcagcatc
     6781 gcgaatggaa gccgtttccg aaataaaatc aggcaacgtt ccgttatcaa tatcttgctg
     6841 ctgctgaatg cgcgctgcca gaagtttatt gcgttgtggc gtaaaatgcg tcaccagctc
     6901 agtcagaaat tctaccgctt cggcagtaag aatttgcttc tcctgctcgc catacggcct
     6961 tgtgaaagcc agttcatcgg ttgttgttgc ctgttcagtc atcgtgcagc tcctcgtcat
     7021 ggatccgaaa acttcccctc tgtgaacgaa ggaacgttgt gcgcttttcg ttcagcacaa
     7081 ctaagactac tcatttaaaa tgcaaaatca aaaacaattt ccattttaat taaaaatact
     7141 tgataactta ttgataacaa tatgaataaa atttattcac ttgctgaggt gcgtttcgga
     7201 aataaaaaag gcacccgaag gtgcctgagg taaggtgctg aatcgcttaa cgatcgacta
     7261 tcacagaaga ttaatccagc gttggattca tgtgccgtag atcgtatggc gtgatctgat
     7321 agacgtaata gttgagccag ttggtaaaca gcaaattacc gtgactacgc cagctcgctc
     7381 gcggtttgtt ttgcggatcg ttgtgcggga aatagttata cggtacatcc gggtctagtc
     7441 cggcttccac atcgcgtaaa tattcctgcg ccagcgtttg cgcatcatat tcgggatggc
     7501 ccgtcacaaa ggcaatgcgc ttatctttgc tggcaaacag atatgcatcc ccttcttccg
     7561 tctctgccag aatttccaga tcggtgtaat cacgaatcaa cgctgccgga aagtcagcat
     7621 agcgcgaatg cggtgccagg aatgaatcat caaagccacg cgtcagaagc gcatgaggat
     7681 ggagaatatg atgctcgtaa acgccagaga gtttgtcggt gcgagtttgc ttaggaatgc
     7741 cgtagaggat attgagcgcg gcctgtaccg cccagcagac aaacagcgtc gaggtgacgt
     7801 gatctttcga ccactccagc acctgtttga tctgcggcca gtaagcgaca tcattaaact
     7861 ccaccaggcc cagcggcgca ccggttacaa tcagaccgtc aaagttctgt tcctgaatat
     7921 cttcaaagtt acagtagaag ttgttcagat gctctgcggg cgtgttgcgc gattcacggg
     7981 aatcgatgcg caacagctga atatcgacct gcaaaggtga gtttgaaagc aggcgcagaa
     8041 actgattttc agtttcaatc ttcttcggca tcaggttaag gatcagaacc ttaagtggac
     8101 gaatttcctg accagacgca cgagaagttg tcatcacaaa gacgttttct tcacgcaaga
     8161 aattgacggc gggtagctcg tccggcacac gaatcggcat aacctgatta cctcactaca
     8221 tacgcttata cgtttagaca tccagatagc tgaagataac cagaaaattt gccaatgtcg
     8281 agcctgcatg ttgaaggtga gaaagtttca aggaaaaggc tacgttagaa tataagaatg
     8341 acgaaaagga gagaaaatgg ttattagtat tcgccgctca cggcatgagg aaggggagga
     8401 actcgttgcg atttggtgtc gatctgtcga tgccacccac gattttttat cagcagagta
     8461 tcgggccgag ctggaagagc tggtgcgttc ctttctgccg gaagcaccgt tgtgggtcgc
     8521 ggttaatgaa cgggagcagc cggttgggtt tatgttgtta agtgggcagc atatggatgc
     8581 gctgtttatc gatcctgatg tgtgcggctg cggcgtgggt cggatgctgg tggagcatgc
     8641 gctctcgatg gcaccggaac tgacaaccaa cgttaatgag caaaatgagc aggcggttgg
     8701 gttctataag aaggtgggtt ttaaggttac gggacgctct gaggtggacg atttggggaa
     8761 accgtatccg ttgctgaatc tggcgtatgt gggggaataa gccgcctctg ccgtgcctgc
     8821 ccaagctgga gaagacgcgc cagcgtcgca cccggcattt ttttcaccaa cgcaaaaagg
     8881 ccatcctgac g
//
LOCUS       DAZPAB010000158         8760 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZPAB010000158 DAZPAB010000000
VERSION     DAZPAB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.158"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..439
                     /locus_tag="ACHUPG_003181"
     CDS             <1..439
                     /locus_tag="ACHUPG_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR0862691.1"
                     /translation="QKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQ
                     FAVQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYA
                     KAKLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="ACHUPG_003182"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="ACHUPG_003182"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR0862692.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="ACHUPG_003183"
     CDS             complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="ACHUPG_003183"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR0862693.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="ACHUPG_003184"
     CDS             complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="ACHUPG_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR0862694.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4441..5148)
                     /locus_tag="ACHUPG_003185"
     CDS             complement(4441..5148)
                     /locus_tag="ACHUPG_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFR0862695.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPGFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="ACHUPG_003186"
     CDS             complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="ACHUPG_003186"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR0862696.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6006..6209)
                     /locus_tag="ACHUPG_003187"
     CDS             complement(6006..6209)
                     /locus_tag="ACHUPG_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0862697.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6368..7876
                     /locus_tag="ACHUPG_003188"
                     /pseudo
     CDS             6368..7876
                     /locus_tag="ACHUPG_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7873..>8760
                     /locus_tag="ACHUPG_003189"
     CDS             7873..>8760
                     /locus_tag="ACHUPG_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFR0862698.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEP"
BASE COUNT         2032 a         2389 c         2251 g         2088 t
ORIGIN      
        1 gcagaaggtg gcgttaaatc tcgcttcagg tgaagggaat ttctttatcc gtcctggcgg
       61 cgtgttttat gtcgcgggag ataaagtcgg catcgttcgt ctggatgctt ttaaagccag
      121 taaagatatt cagtttgcgg tgcagtcagg accaatgttg atggaaaacg gtgtaattaa
      181 tccgcgtatt catcccaacg tcgcctcacg taaaattcgt aacggtgttg ggattaataa
      241 acatgggaat gccgtgtttt tgttgagcca gcaggcgaca aatttttatg attttgcctg
      301 ttatgccaaa gcgaaactga acgttgagca gctgctttat cttggtggca cgatttcaca
      361 tatgtatatg aaaggcgggg caatcccgtg gcaacgctat ccttttgtca ccatgatttc
      421 cgtcgagcgg aaaggttaga atgatagctt tgtgtcagat actaaatatg ctgctgatgc
      481 cggatgcggc gtaaacgcct tatccggcct acatgacgtt gcaatttatt gaatctgaat
      541 gattttttag gccaaataag gtgcgcagca ccgcatccgg caacgttaca ccgactccag
      601 ccgggcgtaa gctgccacca gccatttgat tccctggccc tggaatgcca cctgcaaacg
      661 gctatgttca ccgctgcctt ccatattgac gatggtgcct tcaccaaact tagcgtggcg
      721 tacacgctgg ccgagcttgt agccgctgtc gttctcgacc atcggcgtac ccatccgctg
      781 atggctgacc gggcggctta ccgtggcgcg caggcgcacc tcttccacac actcttccgg
      841 caactcgcca ataaagcgcg acgggcgatg gtaaacctct ttgccataca gacggcgagt
      901 ttccgcgtag gtcagcgtca gtttctgcat cgcgcgggtt acgccaacgt aggccagacg
      961 gcgttcttct tccagacgcc cgccttcatc cagcgacatc tggcttggga acatgccctc
     1021 ttccataccg acgataaaca cctgcgggaa ctccagacct ttcgccgagt gcatggtcat
     1081 cagttgtacc gcatcctgcc aggtatccgc ctgcccttcg cctgcttcca gcgccgcatg
     1141 ggataggaac gcctgcagcg gcattaaatc ttcgtcttct tcgttgtagc tgaactggcg
     1201 cgttgccgtc accagttcct ctaagttttc gatacgcgtc tgaccttttt cgcccttctc
     1261 ctgctcgtac atggtacgca ggccggagtc tttaattacc cggtcagtct gtacatgcag
     1321 cggcatatcg gcagtttcct gcgctaaggc gtcgatcagt tccataaacc gctgtaaggc
     1381 gctggcagca cgtccggcga gggctttttc ctgcaacagt tcacgacatg cctgccagag
     1441 tgttaactgg cgatcgcgcg atgtctgacg taccacgtcc agcgtccggt caccaatacc
     1501 ccgcgttggc gtattcacca cacgctcaaa ggccgcgtcg tcgttacggt tggcaatcag
     1561 gcgcagatac gagagcgcat ctttgatttc ctggcgttcg aagaagcgca tcccgccgta
     1621 aatacggtac ggcatactcg cctgcaacag tgcttcttcc agcacacgcg actgggcgtt
     1681 actgcggtag agaatggcgc actcggcaag cgccccgccg ttgtcctgcc aggttttgat
     1741 gcggttaacc acaaaacgcg cttcatcgag ttcgttaaaa gcgcaataga gggaaatagg
     1801 ctcaccgtcc gcgccatcgg tccacagttt tttacccaga cgcccgttat tgttttcaat
     1861 cagggcgtta gcggcgctca gaatattgct ggtagagcgg tagttttgct ccagacgaat
     1921 aatttcggca ccggggaaat cattaaggaa acgctgaata ttctccacct gcgccccgcg
     1981 ccagccgtag attgactggt cgtcatcacc gacgatcatc actttgccgg tgtcgcccgc
     2041 cagcaggcgg atccacgcgt actgaatgtt gttggtatcc tggaattcgt ccaccaggat
     2101 attggtaaaa cgttcgcggt agtgttgtag gatatgcggc ttgttaagcc acagttcgtg
     2161 agcgcgcagc agcagctcgg cgaagtccac caggcccgcg cgatcgcacg cttcctgata
     2221 cgcctgatac accttctgcc aggtctgctc caccggatta ccgtagcttt gaatatgatg
     2281 cggacgcagg ccttcatctt tctggctgtt gatgtaccac attgcctggc gcggcggcca
     2341 ctgcttctcg tcgaggttca tggctttgat cagacgctta agcaggcgta gctggtcttc
     2401 gctatcgagg atctggaaat cctgcggcag attggcgtcc atatggtgcg cacgcagcag
     2461 gcggtgcgcc agcccgtgga aggtgcctac ccacatgccg ccctggctgg tgcccatcag
     2521 ttgtccgata cgatgacgca tctccgccgc cgctttgttg gtaaacgtca cagccataat
     2581 cgagtatggc gagcagtttt ccacgctcat cagccaggcg atacgatgca ccagtacgcg
     2641 cgtcttacca ctgcccgcgc ccgccagcac cagaaggttg ctgcgtggcg cggccaccgc
     2701 ttcgcgttgt ttgtcattaa ggctgtcgag caggtaagaa acgtccattg gcaccgccgc
     2761 gtaaaaatag gttgggcgga gaagcgcaac gccctccgcc aaaaagctgg gtatatatac
     2821 agatttgctg attatatcag cgaggtcaga gatgccaacc gggaaatttc cagatgtggc
     2881 agtaaacggc tgtcccaggt ttgcatcaga tcgccatttt ccggtctgat ccaacaagcc
     2941 tgcattccgc tgcgaattgc cccacccacg tcagtggtga gatcgtcccc aacatgtaag
     3001 atctcgccga tcggcacgtt gagtttttcc gcagccaaaa agtacatatc gctgaacggt
     3061 tttgagcgcc cgtgcgggcc agcgcgcagc acaaactcaa aataatcccc caaaccaaac
     3121 agttccggct gggcgttacc gttggtgatc gccaccagcg gccatttctt cgccagctgt
     3181 tttaaggtgt cgtgagtttg ctgcgggacg tcgattcggc tgcgccattt ggcaaagtta
     3241 atcattgctg cgtgtgcgcc tgcactggct tcttcggcac tcagcccggc gtcgagcatc
     3301 gcttgttcaa tcgaacgaaa acgccaacgt gtcacgtcgt gataaatctc gggttccgct
     3361 tcccgtaccg cctggcgcag gcgttgcaga tcttcattct ggaagctgcg cagcgccgga
     3421 tgataatttt gcacaaaggt aagcgcctct cgctcggtgc gcaaaatcac cggacggtta
     3481 tcgtaaaggg tatcatccag gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa
     3541 aaacgcatta tttcccccgt ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt
     3601 gttgaaaatc aagatgagta tagatttgcg tggtggagag gttggcatga cccagtagct
     3661 cctgcacacc acgaagatcg ccgctcgact ccagcatatg cgtagcgaac gagtgacgta
     3721 atttatgtgg gtgaacgtga ttattcagcc cttgttttat gccccattcg gcaaagcgtt
     3781 tctgcacatt acgcgcggag atacgcttgc ccagtttcga cagaaaaagc gcgtcgtctt
     3841 cgctaccaaa caggtcgcgc aaatcaagcc agtgctcaat ccacgccaca gcgttgcgac
     3901 caatcggcag gcggcgctct ttgctgcctt tccccatcac ccacacttcg ccggactcca
     3961 ggtcgaggtg tttgatatcc agccccacca gctcggaaag acgcagaccc gcgccgtaca
     4021 tcacttccag cattgcacgg tcgcgtacag cgaggggatc gttgatatca atatccagca
     4081 gccgattcat atcgtcgacg tcgatatttt tcggcagatg acgcggcgct ttcggtgccg
     4141 aaactccttt cgccgggtta gctttgagtt cgttctggct gaccagccag tcaaaaaagc
     4201 tacgtagcgc agaaagccgt aacgccagac ttgctgctcc cagcccttta cggcgactgc
     4261 gtacagcaaa attgcgtacc atcgctgcat cgcattgttg ccagctttgc aggccgtttt
     4321 cgctggcaaa attgatgatc gcctcaagct gacgctggta gttaagtagg gttatcgggc
     4381 taagctgacg ctccacgctc agataacgta ggtagcgttc tacatcggtg tgtaaatcgg
     4441 tcatacgcgt tcaatccaac gctccagaag ctccggcaac atcagcgcaa tttcatgaag
     4501 taactgcgtt ccctgccctt gttgatagtg actggcatcg cgactggtaa acagcacaac
     4561 acccaaatca gcatcgcttc ccagcatcga catcgccacc gatcccaccg ctttcgcttc
     4621 cggtagcacc accagcagct ccggtccgtt aagcggccca agatagtgct gttcctgccc
     4681 caaacgctga atacgcagcg gttcgaaaga ctgacggctt aatgccagat gagtgtggtt
     4741 cgacggcgca cctaagcgcc agcgatctgg aaacaggcgc agactcgcac cagctaggcc
     4801 gagatcgcgc gcccagcggt gaaagcgcat cagcatatcg tcgagactgc tggcggcggt
     4861 gagactgcgc tgcaggtaga gtaggcgata aaacagccct tcgttggcga tagcctgctc
     4921 catcaacaac gccatgttct cttccagaac atgaatgtga ttacgtgcgc gggccatgtg
     4981 ccactcgacc aacgaaacgg tgccgcgtac cggatgcggc acacgtatcg cttctactgc
     5041 gcgcgcattg cggataaaaa acccaggatt tttaatcaga taatcgacaa ccgcccggtc
     5101 atcaagctcc gtgagtgttt cctgcagttc ttcccctggt tgcttcatag atgaataaat
     5161 ccatcgtaga catgtaccgc cgggccagtc atatataacg ggtgacccgg acctttccag
     5221 gcgatatcaa gacgaccgcc ggggagttcc acgcgtactt cttcgtccag caaaccttgc
     5281 tgaatcccta ccgccaccgc cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc
     5341 ccacgctcat aaacgcgtaa acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata
     5401 ttggcgcgct ccggaaaacg ctcgtgactt tccagaacag gaccaagcgt ttctaccgcc
     5461 gcggtatcga catcatcgac ctgaatcacg caatgcggat ttcccatcga caccacgccg
     5521 cataagattg tctgctcggc ggcgcgcata atataggtct tttccgcttt gttagcgcga
     5581 aacggcacgg cggaaggttc gaagttgggt tcgcccatat ttacgcggac cagatcatca
     5641 tcggtgacgg tcagaaccat ccgcccgttg gcggtgctga cgcggatatc acgcttattg
     5701 gtcagtcctt tcagacgcac aaaacgggca aagcagcgcg caccgttacc gcactgcgcc
     5761 acttcactgc catcagcatt gaaaatgcga tagtgaaaat ccagttcagg atcatacggc
     5821 ggctcaacca ccagcagctg gtcaaaccct acccccaggt gccgatccgc caggcgacga
     5881 atcagctccg gtgaaaaaaa gacattctgc gttaccgcgt cgacgaccat aaaatcgttg
     5941 ccaaggccat gcattttcga gaactgcatc atttactcca atcacgcggg tacagaaact
     6001 gacttttagt aattcacctg ggatggacca tcgccagtgg cgcgatcgtt tttatccggc
     6061 accgtggatt gcgtttgcgt ctctaccggt ttggtcggcg gcggtgcgtt tttatctgca
     6121 ggcgggaaat agagcggacc tttcagaccg cagcccgtca ggctgaagag agtaagtaat
     6181 acagtgagtg ccttaaacac gtttttcatt atggattgcc tgtaaagttc atcgctttct
     6241 gctttctatc atcgcaggag aaggcgtaaa agcaagcgtt tagcgactct ttatgaagtc
     6301 tgaaaagtga ggaaatgctg cgagtataac gcttactcgt gaggatttat gttctgctgg
     6361 cagtttcgtg atcaacacct cgcgccagga tggctttatg cactatcgca atgtggtttg
     6421 tttgttgtct tgttcattgt ttttatcgtc ggcatgggga tgtcggcttt ccgcacctga
     6481 acataatatt taccagaaac agggaaaagg cgtggtgtac ctgcgccctt ataaagaaac
     6541 aaatctttct ctacctgacg tgaattacaa gagtttgcgt cggttaccca atttattaat
     6601 agacccgaca acactagacg aatgggataa agaaccgcct ctgactgatt tgacaaccga
     6661 ctacctctat gagggcgcac aggcctggta tcctcattat tcatggcata gcgatggtcg
     6721 caatatcctc tacgcgggtg aagttgtgca aaaccctccc ggcacacctc ccgtcgatgt
     6781 cgcctcgttc aaggcatggg gtgattttgc cgcagataaa cacagtctct attttgaagg
     6841 caagcgcacc gatgacaatg gtggtggaaa tagcttggat atcaaaacgc tgcatcaggt
     6901 ggaattccgg cctccctggg atcctgactt actggggctg atattacgtg acgctaattt
     6961 cttgtatatc aatgggcatc gcctcgctga ccccgagagt ttccgtgtgc tggcgcagaa
     7021 gtcctgggat cagcgaggaa agttttccac cacgtttaac ccctgcattg ccgtaccatt
     7081 tggcccctgg gataccctgg ctcgtacacg gaccaaaatc ctgctcaacg gcgaacagct
     7141 tgatgccgac ccggacacct tttccgtcgt gcgctggatg cccggctcac tcttgacctg
     7201 gcgtgataaa aacgggctgc agcgtaaagt cctcgacaag gaaaatctgg cgtgggatga
     7261 agatttaaca aagcactgtc tggatttttc tctgctggaa aagaaagtgt tctggcgtaa
     7321 aggacctgct tgtaaacagg aagaattacc cggactcgat ccggagcagt ttcaacccat
     7381 cagtgatgct gtcgcccagc atcaggactc gctttatacc atcatcgaaa cagagtctgg
     7441 taaccgtaag ctggagatcg tgaaacttga tgatcccaat cttattatca acaaacgttt
     7501 caacgccggg aaacgccacg gctatttact tacgcgtgcc gaagggtagc cataccattc
     7561 cggtttacac gtgtttgaat ctgacggacc gctgatctta ctggataacc gttctccgga
     7621 tgaacgcgaa gcccatctta atgaccatcc ctttttgcgc agatggtatg cccgcgataa
     7681 ccgctacatt tacagctttg atggcgcgca gctctggcga taccgcaccg ctgatccgaa
     7741 acaagttcgc ttaatctgga aggaacaaca ttcgggatat gtctatggcg taaattacaa
     7801 aacgggatat ctggacggaa aaattactga tgacggcgaa tttattcctg ccccgcgcaa
     7861 tgaggcgaca aaatgaaggg tcaaataatc acagttgcac tcatcctgtt gggacacaca
     7921 tttctgacgc ctgctgtaca ggccataggg ttcgactatt acaatgatca cggtgttatg
     7981 tcttacggta aaggttattg gggggataag aaaatcattg cccagtttcc ggagatgagc
     8041 agggccggtt tgcgccttgt gaaaaatatc tctgggaaaa cgggttatat accttccgat
     8101 gaaataagca tagaaaatga ggaataccac tgggtgacag acggccgcgt tattctctgg
     8161 cgtggcaaaa tcgttagcaa tccaccggga acactcactg tcgatattgc cagctttcag
     8221 gctatgggcc gcttcgcggt cgataaatat agcctctatt ttgacggaca gcgcaccgaa
     8281 agtaatagcg gcgcgtcacg tgttgagctg gcgacactaa aagctatcga aggtaactct
     8341 accacgctgg tggatagtaa aaatctctac ttgtccggtc gacgtcaggg gagcagcagt
     8401 gatgttactg tattagaaaa aagatggtgg ggtattaatc cacgtcttat gagtgtaaac
     8461 agaaattcat attccaatga tctgcttatc cgcagtgggc agaatattta tttaaatggc
     8521 gttcacctta cggcgaatgc agactcattt gagataattc gctggatacc tcattcactg
     8581 ctggtttttc gcgacaataa gggtctgcat cgttatccct ttggtcaatt atcaggcaaa
     8641 gcgatacccg tagatgatga cgtctctttt gaagtagggg aaagtcgcgt tcgctggcgt
     8701 aaacagctca cgcccgaccg tcagtggagc aagtggatag acctaccagg tattgaacct
//
LOCUS       DAZPAB010000159         8690 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZPAB010000159 DAZPAB010000000
VERSION     DAZPAB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.159"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(330..593)
                     /gene="rpsT"
                     /locus_tag="ACHUPG_003190"
     CDS             complement(330..593)
                     /gene="rpsT"
                     /locus_tag="ACHUPG_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR0862699.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            696..914
                     /locus_tag="ACHUPG_003191"
     CDS             696..914
                     /locus_tag="ACHUPG_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR0862700.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            922..1863
                     /gene="ribF"
                     /locus_tag="ACHUPG_003192"
     CDS             922..1863
                     /gene="ribF"
                     /locus_tag="ACHUPG_003192"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR0862701.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1906..4722
                     /gene="ileS"
                     /locus_tag="ACHUPG_003193"
     CDS             1906..4722
                     /gene="ileS"
                     /locus_tag="ACHUPG_003193"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR0862702.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4722..5216
                     /gene="lspA"
                     /locus_tag="ACHUPG_003194"
     CDS             4722..5216
                     /gene="lspA"
                     /locus_tag="ACHUPG_003194"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR0862703.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5303..5752
                     /gene="fkpB"
                     /locus_tag="ACHUPG_003195"
     CDS             5303..5752
                     /gene="fkpB"
                     /locus_tag="ACHUPG_003195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0862704.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5754..6704
                     /gene="ispH"
                     /locus_tag="ACHUPG_003196"
                     /gene_synonym="lytB"
     CDS             5754..6704
                     /gene="ispH"
                     /locus_tag="ACHUPG_003196"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR0862705.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6770..7684
                     /gene="rihC"
                     /locus_tag="ACHUPG_003197"
     CDS             6770..7684
                     /gene="rihC"
                     /locus_tag="ACHUPG_003197"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR0862706.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7851..8672
                     /gene="dapB"
                     /locus_tag="ACHUPG_003198"
     CDS             7851..8672
                     /gene="dapB"
                     /locus_tag="ACHUPG_003198"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR0862707.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
BASE COUNT         1990 a         2160 c         2462 g         2078 t
ORIGIN      
        1 aggtctaatg aggagatatt tatgaatggt taaagtatat aggaatttaa ttcaaaaata
       61 gagtggtctt aagtttaata cctcaatgtg tatcacaata tccatattct ttgtggggga
      121 gtctggagat tgagtagata ttcttgttca gaatgtatca gccgatggtt ctacgattct
      181 taagccacga agagttcaga tagtacaacg gcatgtctct tttgactatc tggcaaccgg
      241 cagtgtgttc tctcacgcat cacaaaagca gcaggcataa aaaaacccgc ttgcgcgggc
      301 tttttcacaa agcttcagca aattggcgat taagccagtt tgttgatctg tgcagtcagg
      361 ttagccttat gacgtgcagc tttgtttttg tggatcagac ctttagcagc ctgacggtcc
      421 acgatcggtt gcatttcgtt aaatgctttc tgtgcagcag ctttgtcgcc agcttcgata
      481 gctgcgtata ctttcttgat gaaagtacgc atcatagagc gacggcttgc gttgtgctta
      541 cgagcctttt cagactgaat ggcgcgcttc ttagctgatt tgatattagc caaggtccaa
      601 ctcccaaatg tgttctatat ggacaattca aaggccgagg aatatgccct tttagccttc
      661 ttttgtcaat ggatttgtgc aaataagcgc cgttaatgtg ccggcactcg ttacgtagtg
      721 atggcgcagg attctaccag cttgcggggt gtgaatacag cttttccgcg ataaaaattg
      781 cagcaggcgg tcagtttctt cccgtgattt gctccatggc aatgaaaagc cacttctttc
      841 tgatttcggt actcaatcgc cggttaacct tgaccgctgt acaaggtata ctcggacgat
      901 tttcactgtt ttgagccaga catgaagctg atacgcggca tacataatct cagccaggcc
      961 ccgcaagaag ggtgtgtgct gactattggt aatttcgacg gcgtgcatcg cggtcatcgc
     1021 gcgctgttac agggcttgca ggaagaaggg cgcaagcgca acttaccggt gatggtgatg
     1081 ctttttgaac ctcaaccact ggaactgttt gctaccgata aagccccggc aagactgacc
     1141 cggctgcggg aaaaactgcg ttaccttgca gagtgtggcg ttgattacgt gctgtgcgtg
     1201 cgtttcgaca ggcgttttgc ggcgttaacc gcgcaaaatt tcatcagcga tcttttggtg
     1261 aagcatttgc gcgtaaaatt tcttgccgta ggtgatgatt tccgctttgg cgctggtcgt
     1321 gaaggcgatt tcttgttatt acagaaagct ggcatggaat acggcttcga tatcaccagt
     1381 acgcaaactt tttgcgaagg tggtgtgcgt atcagcagca ccgccgtgcg tcaggcgctg
     1441 gctgatgaca atctggctct ggcagaaagt ttactggggc acccgtttgc catctccggg
     1501 cgtgtagtcc acggtgatga attagggcgc actataggtt tcccgacggc gaatgtaccg
     1561 ctacgccgtc aggtttcccc ggtgaaaggg gtttatgcgg tagaagtgct gggcctcggt
     1621 gaaaagccgt tacccggcgt ggcaaacatc ggaacacgcc caacggttgc cggtattcgc
     1681 cagcagctgg aagtgcattt gttagatgtt gcaatggacc tttacggtcg ccatatacaa
     1741 gtagtgctgc gtaaaaaaat acgcaatgag cagcgatttg cgtcgctgga cgaactgaaa
     1801 gcgcagattg cgcgtgatga attaaccgcc cgcgaatttt ttgggctaac aaaaccggct
     1861 taagcctgtt atgtaatcaa accgaaacac ggaaccgaga atctgatgag tgactataaa
     1921 tcaaccctga atttgccgga aacagggttc ccgatgcgtg gcgatctcgc caagcgcgaa
     1981 ccgggaatgc tggcgcgttg gactgatgat gatctgtacg gcatcatccg tgcggctaaa
     2041 aaaggcaaaa aaaccttcat tctgcatgat ggccctcctt atgcgaatgg cagcattcat
     2101 attggtcact cggttaacaa gattctgaaa gacattatcg tgaagtccaa agggctttcc
     2161 ggttatgact cgccgtatgt gcctggctgg gactgccacg gtctgccgat cgagctgaaa
     2221 gtagagcaag aatacggtaa gccgggtgag aaattcaccg ccgccgagtt ccgcgccaag
     2281 tgccgcgaat acgcggcgac ccaggttgac ggtcaacgca aagactttat ccgtctgggc
     2341 gtgctgggcg actggtcgca cccgtatctg actatggact tcaaaactga agccaacatc
     2401 atccgcgcgc tgggcaaaat catcggcaat ggtcacctgc acaaaggcgc gaagccggtg
     2461 cactggtgcg tagactgccg ttctgcactg gcagaagcgg aagttgagta ttacgacaaa
     2521 acttctccgt ccattgacgt cgctttccag gcggtcgatc aggatgcgct gaaagcgaaa
     2581 tttggcgtaa gcaacgttaa cggcccaatc tcgctggtga tctggaccac tacgccgtgg
     2641 actctgcctg cgaaccgcgc aatctctatt gcacctgatt tcgactatgc gctggtgcag
     2701 atcgacggtc aggccgtgat tctggcgaaa gatctggttg aaagcgtaat gcagcgtatc
     2761 ggcgtgaccg attacaccat tctcggcacg gtaaaaggtg cagagcttga gctgctgcgc
     2821 tttacccatc cgtttatggg cttcgacgtt ccggcaatcc tcggcgatca cgttaccctg
     2881 gatgccggta ccggtgccgt tcacaccgcg cctggccacg gcccggacga ctatgtgatc
     2941 ggtcagaaat acggcctgga aaccgctaac ccggttggcc cggacggcac ttatctgccg
     3001 ggcacttatc cgacgctgga tggcgtgaac gtcttcaaag cgaacgacat cgtcgttgcg
     3061 ctgctgcagg aaaaaggcgc gctgctgcac gttgagaaaa tgcagcacag ctatccgtgc
     3121 tgctggcgtc acaaaacgcc gatcatcttc cgcgcgacac cgcagtggtt cgtcagcatg
     3181 gatcagaaag gtctgcgtgc gcagtcactg aaagagatca aaggcgtgca gtggctcccg
     3241 gactggggcc aggcgcgtat cgagtcgatg gttgctaacc gtcctgactg gtgtatctcc
     3301 cgtcagcgca cctggggcgt accgatgtca ctgttcgtgc acaaagacac ggaagagctg
     3361 catccgcgta ccctcgaact gatggaagaa gtggctaaac gcgttgaagt tgacggcatc
     3421 caggcgtggt gggatctcga tgcgaaagag atcctcggcg acgaagctga ccagtatgtg
     3481 aaagtgccgg atactctcga cgtatggttt gactccggat ctacccactc ttctgttgtt
     3541 gacgtgcgtc cggaatttgc cggtcacgca gcggacatgt atctggaagg ttctgaccag
     3601 caccgtggtt ggttcatgtc ttccctaatg atctccaccg cgatgaaagg caaagcgccg
     3661 tatcgtcagg tactgaccca cggctttacc gtggatggtc agggtcgcaa gatgtctaaa
     3721 tccatcggca acaccgtttc gccgcaggat gtgatgaaca aactgggcgc ggatattctg
     3781 cgtctgtggg tggcatcaac cgactacacc ggtgaaatgg ccgtttctga cgagatcctg
     3841 aaacgtgctg ccgatagcta tcgtcgtatc cgtaacaccg cgcgcttcct gctggcaaac
     3901 ctgaatggtt ttgatccagc aaaagatatg gtgaaaccgg aagagatggt ggtactggat
     3961 cgctgggccg taggttgtgc gaaagcggca caggaagaca tcctcaaggc gtacgaagca
     4021 tacgatttcc acgaagtggt acagcgtctg atgcgcttct gctccgttga gatgggttcc
     4081 ttctacctcg acatcatcaa agaccgtcag tacaccgcca aagcggatag cgtggcgcgt
     4141 cgtagctgcc agactgcgct gtatcacatc gctgaagcgc tggtgcgctg gatggcacca
     4201 atcctctcct tcaccgctga tgaagtgtgg ggctatctgc cgggcgaacg tgaaaaatac
     4261 gtcttcaccg gtgagtggta cgaaggcctg tttggtctgg cagacagtga agcaatgaac
     4321 gatgcgttct gggacgagct gttgaaagtg cgtggcgaag tgaacaaagt cattgagcaa
     4381 gcgcgtgccg acaagaaagt gggtggctcg ctggaagcgg cagtaacctt gtatgcagaa
     4441 ccggaactgg cggcgaaact gaccgcgctg ggcgatgaat tacgatttgt cctgttgacc
     4501 tccggcgcta ccgttgcaga ctataacgac gcacctgctg atgctcagca gagcgaagta
     4561 ctcaaagggc tgaaagtcgc gttgagtaaa gccgaaggtg agaagtgccc acgctgctgg
     4621 cactacaccc aggatgtcgg caaggtggcg gaacacgcag aaatctgcgg ccgctgtgtc
     4681 agcaacgtcg ccggtgacgg tgaaaaacgt aagtttgcct gatgagtcaa tcgatctgtt
     4741 caacagggct acgctggctg tggctggtgg tagtcgtgct gattatcgat ctgggcagca
     4801 aatacctgat cctccagaac tttgctctgg gggatacggt cccgctgttc ccgtcgctta
     4861 atctgcatta tgcgcgtaac tatggcgcgg cgtttagttt ccttgccgat agcggcggct
     4921 ggcagcgttg gttctttgcc ggtattgcga ttggtattag cgtgctcctg gcagtgatga
     4981 tgtatcgctc gaaggccacg cagaagctaa acaatatcgc ttacgcgctg attattggcg
     5041 gcgcgctggg caacctgttc gaccgcctgt ggcacggctt cgttgtcgat atgatcgact
     5101 tctacgtcgg cgactggcac ttcgccacct tcaaccttgc cgatactgcc atttgtgtcg
     5161 gtgcggcact gatcgtgctg gaagggtttt taccttccaa agcgaaaaaa caataataaa
     5221 tcctgccgga tgcggcctac agattgctac gaaatcgtag gccgcatccg gcaaaaatcc
     5281 tcaaaaataa gagcaaacct gcatgtctga atctgtacag agcaatagcg ccgtcctggt
     5341 gcacttcacg ctaaaactcg acgatggcac caccgccgag tctactcgca acaacggtaa
     5401 accggcgctg ttccgcctgg gcgatgcttc tctttctgaa gggctggagc aacacctgct
     5461 ggggctgaaa gtgggcgaca aaaccacctt ctcgctggag ccagatgcgg cgtttggcgt
     5521 gccgtcaccg gacctgattc agtacttctc ccgccgtgaa tttatggatg caggcgagcc
     5581 agaaattggc gcaatcatgc tttttaccgc aatggatggc agtgagatgc ctggtgtgat
     5641 ccgcgaaatt aacggcgact ccattaccgt tgatttcaac catccgctgg ccgggcagac
     5701 cgttcatttt gatattgaag tgctggaaat cgatccggca ctggaggcgt aacatgcaga
     5761 tcctgttggc caacccgcgt ggtttttgtg ccggggtaga ccgcgctatc agcattgttg
     5821 aaaacgcgct ggccatttac ggcgcaccga tatatgtccg tcacgaagtg gtgcataacc
     5881 gctacgtggt cgatagcttg cgcgagcgtg gggctatctt tattgagcag attagcgaag
     5941 tgccggacgg cgcgatcctg attttctccg cacacggtgt ttctcaggcg gttcgtaacg
     6001 aagcgaaaag ccgcgatttg acggtgtttg atgccacctg tccgctggtg accaaagtgc
     6061 atatggaagt tgcccgcgcc agtcgccgtg gcgaagaatc tattctcatc ggccacgccg
     6121 ggcacccgga agtggaaggg acgatggggc agtacagcaa ccccgaaggg ggaatgtatc
     6181 tagtcgaatc gccagacgat gtgtggaaac tgacggtcaa aaacgaagag aagctctcct
     6241 ttatgaccca aaccacgctg tcggtggatg acacgtctga tgtgatcgac gcgctgcgta
     6301 aacgcttccc gaaaattgtc ggtccgcgca aagatgacat ctgctacgcc acgactaacc
     6361 gtcaggaagc ggtacgcgcc ctggcagaac aggcggaagt tgtgttggtg gtcggttcga
     6421 aaaactcctc caactccaac cgtctggcgg agctggccca gcgtatgggc aaacacgcgt
     6481 ttttgattga cgatgcgaaa gatatccagg aagagtgggt gaaagaggtt aaatgcgtcg
     6541 gcgtgactgc gggcgcatcg gctccggata ttctggtgca gaatgtggtg gcacgtttgc
     6601 agcagttggg tggtggtgaa gccattccgc tggaaggccg tgaagaaaat attgttttcg
     6661 aagtgccgaa agagctgcgt gtcgatattc gtgaagtcga ttaagtcatt agcagcctaa
     6721 gttatgcgaa aatgccggtc ttgttaccgg cattttttat gaagaaaaca tgcgtttacc
     6781 tatcttcctc gatactgacc ccggcattga cgatgccgtc gccattgccg ccgcgatttt
     6841 tgcccccgaa ctcgacctgc aactgatgac caccgtcgcg ggtaatgtct cggttgagaa
     6901 aactacccgc aatgccctgc aactgctgca tttctggaat gcggagattc cgctcgccca
     6961 gggagccgct gtgccgctgg tacgcgcacc gcgtgatgca gcatctgtgc acggcgaatc
     7021 gggaatggct ggctacgact ttgttgagca caaccgaaag ccactcggga taccggcgtt
     7081 tctggcgatt cgggatgccc tgatgcgtgc accagagccc gttaccctgg tggccatcgg
     7141 cccgttaacc aatattgcgc tgttactttt acaatgcccg gaatgtaaac cgtatattcg
     7201 tcgtctggtg atcatgggcg gttctgctgg tcgcggcaac tgtacaccaa atgccgagtt
     7261 taatatcgct gccgatccag aagcggctgc ctgtgtcttc cgtagcggca tcgaaattgt
     7321 catgtgcggt ctggatgtca ccaatcaggc aatattaacc cctgactatc tcgctacttt
     7381 accggagttg aatcgcaccg gaaaaatgct ccacgccctg tttagccact accgtagcgg
     7441 cagtatgcaa agcggtctgc gaatgcacga tctctgcgcc atcgcctggc tggtgcgccc
     7501 ggacttattc accctcaagc cgtgttttgt cgcggtagaa actcaaggcg aatttacctc
     7561 aggcacgacg gtggttgata tcgacggttg cctgggcaaa ccagccaatg tacaggtggc
     7621 attggatctg aatgtaaaag gcttccaaca gtgggtggct gaggtgctgg ctctggtgcc
     7681 gtaacctgtc acatgttatc ggcatgcagt cattcatcga ctcatgcctt tcactgatat
     7741 ccctccctgt ttatcattaa tttctaatta tcagcgtttt tggctggcgg cgtagcgatg
     7801 cgctggttac tctgaaaacg gtctatgcaa attaacaaaa gagaatagct atgcatgatg
     7861 caaacatccg cgttgccatc gcgggagccg gggggcgtat gggccgccag ttgattcagg
     7921 cggcactggc attagaaggc gtgcagctgg gcgcggcgct ggagcgcgaa ggatcttctt
     7981 tactgggcag cgacgccggt gagctggctg gtgcagggaa aacaggcgtt accgtgcaaa
     8041 gcagtcttga tgcgataaaa gatgattttg atgtgtttat cgattttacc cgtccggaag
     8101 gtacgctgaa ccatctcgct ttttgtcggc agcatggcaa agggatggtg atcggcacta
     8161 cggggtttga cgaagccggt aaacaggcaa ttcgtgacgc cgccgccgat attgcgattg
     8221 tctttgccgc caattttagc gttggcgtta acgtcatgct taagctgctg gagaaagcag
     8281 ccaaagtgat gggtgactac accgatatcg aaattattga agcacatcat agacataaag
     8341 ttgatgcgcc gtcaggcacc gcactggcaa tgggagaggc gatcgcccac gcccttgata
     8401 aagatctgaa agattgcgcg gtctacagtc gtgaaggcca taccggtgaa cgtgtgccag
     8461 gtaccattgg ttttgctacc gtgcgtgcag gtgacatcgt tggcgaacat accgcgatgt
     8521 ttgccgatat tggcgagcgt ctggagatca cccataaggc gtccagccgc atgacatttg
     8581 ctaacggtgc ggtaagatcg gctttgtggt tgagtggtaa ggaaagtggt ctttttgata
     8641 tgcgagatgt gcttgatctc aataatttgt aaccacaaaa tgtttattat
//
LOCUS       DAZPAB010000160         8528 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZPAB010000160 DAZPAB010000000
VERSION     DAZPAB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.160"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..189
                     /gene="csgC"
                     /locus_tag="ACHUPG_003199"
     CDS             <1..189
                     /gene="csgC"
                     /locus_tag="ACHUPG_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR0862708.1"
                     /translation="GSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVS
                     DGQALHLSQQWTPSSEKS"
     gene            310..621
                     /gene="ymdA"
                     /locus_tag="ACHUPG_003200"
     CDS             310..621
                     /gene="ymdA"
                     /locus_tag="ACHUPG_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR0862709.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            716..1249
                     /gene="ymdB"
                     /locus_tag="ACHUPG_003201"
     CDS             716..1249
                     /gene="ymdB"
                     /locus_tag="ACHUPG_003201"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR0862710.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1191..2672
                     /gene="clsC"
                     /locus_tag="ACHUPG_003202"
     CDS             1191..2672
                     /gene="clsC"
                     /locus_tag="ACHUPG_003202"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR0862711.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2680..3837)
                     /gene="mdoC"
                     /locus_tag="ACHUPG_003203"
     CDS             complement(2680..3837)
                     /gene="mdoC"
                     /locus_tag="ACHUPG_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR0862712.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4231..5766
                     /gene="mdoG"
                     /locus_tag="ACHUPG_003204"
     CDS             4231..5766
                     /gene="mdoG"
                     /locus_tag="ACHUPG_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR0862713.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5759..8302
                     /gene="mdoH"
                     /locus_tag="ACHUPG_003205"
     CDS             5759..8302
                     /gene="mdoH"
                     /locus_tag="ACHUPG_003205"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR0862714.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2150 a         2025 c         2222 g         2131 t
ORIGIN      
        1 ggcagttcag ggcaaagtca gacgaagcaa gaaaagaccc tctcattgcc tgctaatcaa
       61 cccattgctt tgacgaagtt gagtttaaat atttccccgg aagatcgggt gaaaatagtt
      121 gttactgttt ctgatggaca ggcacttcat ttatcacaac aatggacgcc ctcttcagaa
      181 aagtcttaat ttgttgaaat atcgagcata agatgaatct ggagagaatg gtctgctgcg
      241 aatcagccaa cctgaaagta tggataacac aaccttcaag gatgactaat cattgaggaa
      301 atagaataaa tgttcagacc ttttttagac tctcttatgc tcggcagttt gttttttcct
      361 tttattgcca ttgcaggaag caccgcgcaa gggggcgtga tccattttta tggccaaatt
      421 gtggaaccgg catgtgacgt cagcacccag tcatcacccg tagaaatgaa ctgcccacag
      481 aatggttcta ttccgggcaa aacctactcc agcaaagcgt taatgagcgg caatgtcaaa
      541 aacgcgcaaa tagcctcagt aaaagtgcag tatcttgata aacagaaaaa gctggctgta
      601 atgaacatcg aatataacta agtttctgaa caactcacgt tgctgagcag aaaaatgcga
      661 tttaaccaaa aagcctgctg tacacttaag aaacaagaag gtgtaaggag gcgttatgaa
      721 aacgcgtatt catgttgtgc agggtgatat taccaaactg gccgttgatg tgattgtgaa
      781 tgcggctaat ccgtcattaa tgggaggcgg cggcgtcgat ggggccattc atcgcgcagc
      841 gggtccggcc ctgctggatg cctgtttaaa agtcaggcaa cagcagggcg attgccctac
      901 ggggcatgcc gttattacgc ttgcaggcga tcttcccgct aaagccgtag tacacaccgt
      961 cgggccagtc tggcgtggtg gggaacaaaa cgaagaccag cttttgcagg atgcctatct
     1021 gaatagccta cgactggtgg cggcaaacag ctatacgtca gtggcttttc ctgcaatcag
     1081 tactggggtt tatagttacc ctcgtgcggc agcggctgaa atcgcagtta aaaccgtttc
     1141 agaatttatt acccgtcacg ctttacccga acaggtatac tttgtctgtt atgatgaaga
     1201 aaacgcccac ctctacgaaa gactccttac ccaacaagga gatgaatgat ttgccccggc
     1261 tggcgagcgc ggtgctgcca ctgtgttcgc aacatcccgg tcagtgtggt cttttccctc
     1321 tggagaaaag tctggatgcg tttgccgccc ggtatcgtct ggccgaaatg gcagagcata
     1381 cgctggatgt tcagtattac atctggcagg acgatatgtc ggggcggtta ctgttttccg
     1441 ccctgttagc cgcagcaaag cgtggcgttc gcgtccgttt gttgctggac gacaacaata
     1501 cgcccggact tgacgacatt ttgcgcttgc ttgacagtca tccacgcatt gaagtccggc
     1561 tttttaatcc tttctcgttt cgcttgctac gtccgcttgg atatatcacc gacttttccc
     1621 gtctcaatcg ccggatgcac aataaaagtt tcactgtcga tggcgtggtt accctagtgg
     1681 gaggacgaaa tattggtgat gcctattttg gtgcagggga agagccactt ttttcggatt
     1741 tagatgtcat ggcaataggc cccgtggtag aggatgttgc cgatgatttc gcccgctact
     1801 ggtattgcaa atcggtttca cccttacagc aggtgctgga tgtcccggaa ggtgaaatgg
     1861 cggatcgcat cgagttaccc gcctcctggc ataacgatgc catgacgcat cgttatttac
     1921 gcaaaatgga atccagtcca tttataaatc atctggttga tggaacgttg ccgcttatct
     1981 gggcgaagac acgtttatta agtgatgatc cggcgaaagg ggagggcaag gcaaaacggc
     2041 attcactgtt accgcagcgc ctgttcgata tcatgggctc acccagtgaa cgcatcgata
     2101 ttatctcttc ctattttgta ccgacacgcg caggtgtggc gcaactctta cggatggtga
     2161 gaaaaggggt aaagattgcg atcctaacca attctcttgc cgctaacgat gttgctgtcg
     2221 tccatgccgg atacgcgcgc tggcgcaaaa aattgctccg ctatggcgtg gaattatatg
     2281 aactcaagcc gacgcgtgaa caaagcagta cgttacacga tcgcggcata accggtaatt
     2341 ccggtgccag cctgcatgct aaaactttta gcatcgatgg taaaacggtg tttatcggct
     2401 ctttcaattt cgatccgcgt tcaacattgc tcaatactga aatgggcttc gtgatagaga
     2461 gcgaaacgct ggcacagtta attgataaac gctttattca gagccagtac gatgcggcct
     2521 ggcagctccg tctggacagg tggggacgga tcaactgggt tgatcgtcat gcaaagaaag
     2581 aggttattct caaaaaagaa cccgccacca gtttctggaa gcgggttatg gtcagactgg
     2641 cgtcgatatt gcccgtggaa tggttattgt aaatgtggct taacgggctg gtgctttatc
     2701 gttctcacgc ttaacaaccg gtttaccaga gaacaaaaac ttcagtaacg ggatgcgtaa
     2761 atgaatttca tacagaatta tcgcaatccc tactacgaat atcaggccac agagaaaacc
     2821 aagccagttg gaggtgatgt gcggtgtaat gtatgcgccg aaaaacagcg ttaacgggtg
     2881 gtgaaccaga tagataaaca gcgatgcgtt aacaaaataa gtcacccgcg ctgactggaa
     2941 gttaagcaaa cggtggccaa aggagaagac cacattcacc atccacaggc cgaggaccat
     3001 ggtgatcacc gactcggttt cgtacatcca ggcatcgcca ctgccatagc gctggttgag
     3061 taaataagcg acaaacgcca atgctgcagc gagggtacag ccacgagacg gcgtggtaaa
     3121 caaggcttta agatgaggga aaatgaaagc cagtgcgccg aggataaaga acggtaaata
     3181 aaatagcgtt tgcatgacaa taaaattgaa catgccatta ctcagaatgg gtggatacac
     3241 aataaaaatc gttcttctta ttaccgcata gccgatgccg aggcataaaa aaatcaccga
     3301 tagttttacc atcgagaatt ttttattcgt tttatcagaa ttttctaaat tatttctgat
     3361 gcgcttaaat atccatacac acagcgtcgt catgactact aacaccagta aaaaccacag
     3421 gtgtgatatt aattcccagg ccaacgtatt atatttgtca tacaatgata gcccaggcca
     3481 actttccgct ttccctttga catattgcag cataataaat tgcggtaatg tcagtagggg
     3541 gatggctgtt aacatcggga tacctacacg ttcgacacgt actttccacc attttttcaa
     3601 gggatagcgt aaaaaaagca tgtaggaaaa gtatccggat ataacgaaaa atacctgcat
     3661 gcggaacgag tggatgaagt cattaaaaag ggttagccac aatgacggtt cggcgctatt
     3721 cacatgccat gtatggctcg aatagattaa agaaatatga aaagggatcc ctaacaacat
     3781 cagccaggcg cggatggagt cgaggaaata ttcacgttgc gcgggtactg gattcatata
     3841 tggttaacta atctcggatt tttcgtctta tccctgtcgg gttatgcctt taggcttgtt
     3901 gccatagcga caccgacctg accgcgccag gcgcaggctt caaggttttt atgcatagca
     3961 tcatcgctac cactaaccag aatggaagcg tctgtaagac ggttgataaa taaatttgct
     4021 ggcaaaccct acacgaagtc gatgcttctg tctttaggag aagcacggaa agtgaaaacg
     4081 gttgcaatca ggtgcttaat ccatgagcca gcgtgctgaa cgataccggg attcagttgt
     4141 cggaatggct ggttatccat taaaatagat cggatcgata taagcacaca aagggggaag
     4201 tgcttactaa ttatgaaaca taaactacaa atgatgaaaa tgcgttggtt gagtgctgca
     4261 gtaatgttaa ccctgtatac atcttcaagc tgggctttca gtattgatga tgtcgcaaag
     4321 caagctcaat ccttagccgg gaaaggctat gaggcgccta aaagcaactt gccctccgtt
     4381 ttccgcgaca tgaaatacgc ggactatcag cagatccagt ttaatcatga caaagcgtac
     4441 tggaacaatc tgaagacccc attcaaactc gagttctacc atcagggtat gtacttcgat
     4501 accccggtca aaataaatga agtgactgcc accgcagtca aacgaatcaa atacagcccg
     4561 gattatttca ctttcggcga tgttcagcat gacaaagaca cggtaaaaga ccttggtttt
     4621 gccggtttta aagtgcttta cccgatcaac agcaaagata aaaacgatga aatcgtcagc
     4681 atgctcgggg ccagctattt ccgcgtgatt ggtgcaggtc aggtttatgg cctttctgcc
     4741 cgcggcctag caattgatac cgctttgcca tcgggtgaag aatttccacg cttcaaagag
     4801 ttctggatcg agcgtccaaa accgactgat aaacgtttaa ccatttatgc attgcttgac
     4861 tcgccgcgtg cgacaggtgc ttacaaattc gtggttatgc cagggcgtga cacggttgtg
     4921 gatgtgcagt cgaaaatcta tctgcgcgat aaagtcggca aactgggggt tgcaccgtta
     4981 accagtatgt tcctgtttgg gccgaaccaa ccgtcgcctg caaataacta tcgtccggag
     5041 ttgcacgact ctaacggtct gtctatccat gctggtaatg gcgaatggat ctggcgtccg
     5101 ttgaataacc cgaaacattt agcggtcagc agcttctcca tggaaaaccc gcgaggcttt
     5161 ggtctgttgc aacgaggtcg tgatttctcc cgctttgaag atctcgatga tcgttacgat
     5221 ctccgtccaa gcgcatgggt gactccgaaa ggggagtggg gcaaaggcag cgttgagctg
     5281 gtggaaattc caaccaacga tgaaaccaac gataacatcg tcgcttactg gacgccggat
     5341 cagctgccgg agccgggtaa agagatgaac tttaaataca ccatcacctt cagccgtgat
     5401 gaagacaaac tgcatgcgcc agataacgca tgggtgcaac aaacgcgtcg ttcaacgggg
     5461 gatgtgaagc agtcgaacct gattcgccag cctgacggta ctatcgcctt tgtggtcgat
     5521 tttaccggcg cagagatgaa aaaactgcca gaggataccc cggtcacagc gcaaaccagc
     5581 attggtgata atggtgagat agttgaaagc acggtgcgct ataacccggt taccaaaggc
     5641 tggcgtctgg tgatgcgtgt gaaagtgaaa gatgccaaga aaatcactga aatgcgtgct
     5701 gcgctggtga atgccgatca gacgttgagt gaaacctgga gctaccagtt acctgccaat
     5761 gaataagaca actgagtaca ttgacgcaat gcccatcgcc gcaagcgaga aagcagcatt
     5821 gccgaagact gatatccgcg ccgttcatca ggcgctggat gccgaacacc gcacctgggc
     5881 gcgggaggat gattccccgc aaggctcggt aaaggcgcgt ctggaacaag cctggccaga
     5941 ttcacttgct gatggacagt taattaaaga cgacgaaggg cgcgatcagc ttaaggcgat
     6001 gccagaagca aaacgctcct cgatgtttcc cgacccgtgg cgtaccaacc cggtaggccg
     6061 tttctgggat cgcctgcgtg gacgcgatgt cacgccgcgc tatctggctc gtttgaccaa
     6121 agaagagcag gagagcgagc aaaagtggcg taccgttggt accatccgcc gttacattct
     6181 gttgatcctg acgctcgcgc aaactgttgt cgcgacctgg tatatgaaga ccattcttcc
     6241 ttatcagggt tgggcgctga ttaatcctat ggatatggtt ggtcaggatt tgtgggtttc
     6301 ctttatgcag cttctgcctt atatgctgca aaccggtatc ctgatccttt ttgcggtact
     6361 gttctgttgg gtgtccgccg gattctggac ggcgttgatg ggcttcctgc aactgcttat
     6421 tggtcgcgat aaatacagta tatctgcgtc aacagttggc gatgaaccat taaacccgga
     6481 gcatcgcacg gcgttgatca tgcctatctg taacgaagac gtgaaccgtg tttttgctgg
     6541 cttgcgtgca acgtgggaat cagtaaaagc caccgggaat gccaaacact ttgatgtcta
     6601 cattctgagt gacagttata acccggatat ctgcgtcgca gagcaaaaag cctggatgga
     6661 gcttatcgct gaagtcggtg gcgaaggtca gattttctat cgccgccgcc gtcgccgcgt
     6721 gaagcgtaaa agcggtaata tcgatgactt ctgccgtcgc tggggcagcc agtacagcta
     6781 catggtggtg ctggatgctg actcggtaat gaccggtgat tgtttatgcg gcctggtgcg
     6841 cttgatggaa gccaacccga acgccgggat cattcagtcg tcgccgaaag cgtccggtat
     6901 ggatacgctg tatgcgcgct gtcagcagtt cgcgacccgc gtgtatgggc cactgtttac
     6961 agccggtttg cacttctggc aacttggcga gtcgcactac tggggacata acgcgattat
     7021 ccgcgtgaaa ccatttatcg agcactgcgc actggctccg ctgccgggcg aaggttcctt
     7081 tgccggttca atcctgtcac atgacttcgt agaagcggcg ttgatgcgcc gtgcaggttg
     7141 gggggtctgg attgcttacg atctcccggg ttcttatgaa gaattgccgc ctaacttgct
     7201 tgatgagcta aaacgtgacc gccgctggtg ccacggtaac ctgatgaact tccgtctgtt
     7261 cctggttaag ggtatgcacc cggttcaccg tgcggtgttc ctgacgggcg tgatgtctta
     7321 tctctccgct ccgctgtggt ttatgttcct cgcgctctct actgcattgc aggtagtgca
     7381 tgcgttgacc gaaccgcaat acttcctgca accacggcag ttgttcccgg tgtggccgca
     7441 gtggcgtcct gagctggcga ttgcactttt tgcttcgacc atggtgctgt tgttcctgcc
     7501 gaagttattg agcattttgc ttatctggtg caaaggaacg aaagaatacg gcggcttctg
     7561 gcgcgttaca ttatcgttgc tgctggaagt gctgttttcc gtgctgctgg ctccggtacg
     7621 catgctgttc catacggtct tcgttgtcag cgcgttcctt ggctgggaag tggtgtggaa
     7681 ttcaccgcag cgtgatgatg actccacttc atggggcgaa gcgttcaaac gccacggctc
     7741 acagctgctg ttggggttag tgtgggctgt tgggatggcg tggctggatc tgcgtttcct
     7801 gttctggctg gcaccgattg tcttctcgtt gatcctgtca ccgtttgttt cggtgatttc
     7861 cagccgtgcc accgttggtc tgcgcaccaa acgctggaaa ctgttcctga tcccggaaga
     7921 gtattcaccg ccgcaggtgc tggttgatac cgatcggttc cttgagatga atcgccaacg
     7981 ctcccttgac gatggcttta tgcacgcagt gtttaacccg tcatttaacg ctctggcaac
     8041 cgcaatggcg acagcgcgtc accgcgccag taaggtgctg gaaatcgccc gtgaccgcca
     8101 cgttgaacag gcgcttaacg agacgccaga gaagctgaat cgcgatcgtc gcctggtgct
     8161 gctaagcgat ccggtgacga tggcccgtct gcatttccgc gtctggaatt cccctgaaag
     8221 atattcttca tgggtgagtt attacgaagg gataaagctc aatccactgg cattgcgtaa
     8281 accggatgcg gcttcgcaat aaaaacgtag ttgcctgatg cgctacgctt atcaggccta
     8341 catcgttcct gcaatttatt gattttgcaa gattttgtag gtcggataag gcgttcacgc
     8401 cacatccggc aaaaacaacg agccaataaa aataccggcg ttatgccgta atgccagtca
     8461 gttaagcaac tgactggctc tttttcgggg ctgtggggta tttccagggc ctctccttta
     8521 ccactctc
//
LOCUS       DAZPAB010000161         8515 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZPAB010000161 DAZPAB010000000
VERSION     DAZPAB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.161"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..688
                     /locus_tag="ACHUPG_003206"
     CDS             <1..688
                     /locus_tag="ACHUPG_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFR0862715.1"
                     /translation="VKLVMQLKILNIVNKNFIENMKKTFSHCPYIKDPIIRSYIHSGE
                     DDKFDNFMRQHRFSKVNFDTQQMIHFINRFNMNKGLVDKNNNFFIQLIDQALRSTDDM
                     IKANAWYLYKEWIRSDDVSPIFIETEEKLRTFNTNKLTRNDNIFILFSSVDDGPVMVV
                     SSQRLHDMLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYALFPIFTASW
                     RATRIKNKGI"
     gene            911..1408
                     /gene="ubiC"
                     /locus_tag="ACHUPG_003207"
     CDS             911..1408
                     /gene="ubiC"
                     /locus_tag="ACHUPG_003207"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR0862716.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1421..2293
                     /gene="ubiA"
                     /locus_tag="ACHUPG_003208"
     CDS             1421..2293
                     /gene="ubiA"
                     /locus_tag="ACHUPG_003208"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR0862717.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2448..4871)
                     /gene="plsB"
                     /locus_tag="ACHUPG_003209"
     CDS             complement(2448..4871)
                     /gene="plsB"
                     /locus_tag="ACHUPG_003209"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR0862718.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            5042..5410
                     /gene="dgkA"
                     /locus_tag="ACHUPG_003210"
     CDS             5042..5410
                     /gene="dgkA"
                     /locus_tag="ACHUPG_003210"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR0862719.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5520..6128
                     /gene="lexA"
                     /locus_tag="ACHUPG_003211"
     CDS             5520..6128
                     /gene="lexA"
                     /locus_tag="ACHUPG_003211"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR0862720.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6201..7526
                     /gene="dinF"
                     /locus_tag="ACHUPG_003212"
     CDS             6201..7526
                     /gene="dinF"
                     /locus_tag="ACHUPG_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR0862721.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7642..7851
                     /locus_tag="ACHUPG_003213"
     CDS             7642..7851
                     /locus_tag="ACHUPG_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR0862722.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7893..8408)
                     /gene="zur"
                     /locus_tag="ACHUPG_003214"
     CDS             complement(7893..8408)
                     /gene="zur"
                     /locus_tag="ACHUPG_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR0862723.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1976 a         2039 c         2289 g         2211 t
ORIGIN      
        1 agtaaaactg gtgatgcaat taaaaattct caatattgtt aataaaaact ttattgagaa
       61 tatgaagaaa acatttagcc attgtcctta tataaaagat ccaattatac gtagttatat
      121 acattctggg gaagatgaca agttcgataa ttttatgcgt caacatcgat tcagtaaggt
      181 gaatttcgat acccaacaga tgatccattt tattaacaga tttaatatga ataaagggct
      241 ggttgataaa aataacaatt tttttatcca acttatcgat caggccttac gatcaacgga
      301 tgatatgatc aaagcaaatg cctggtatct ttataaagag tggattcgta gtgatgatgt
      361 ttcacctata tttatagaaa ctgaagaaaa gttaagaacc tttaacacta ataaattaac
      421 acgaaacgat aatatattta tcctgttctc ctctgtcgat gatgggcctg ttatggtggt
      481 aagctcccag cgcttacatg atatgttgaa tcctacaaaa gataccaatt ggaattccac
      541 gtgtatctat aaatccagac ataagatgtt gcctattaat cttactcagg aaacactttt
      601 cagctccaaa tctcatggta aatatgcgct tttcccaatt tttactgcga gttggcgagc
      661 tactcgtata aagaataaag gtatttaagt aaaggaaaac atcaccgttc ctagcatcat
      721 gggcggtgat gcccctatgg ttgccctcgc cagcacgggc atcggcaaag cgtaaggttc
      781 aacagcgttt taccacttca tgcgattgtt gcgtttttgt tgcgcattag atcacttaat
      841 ttgctttaca tctcccgtaa acacttttct gcgatacaat gcctttacgt tatgtaacgg
      901 agagttcggc atgtcacacc ccgcgttaac gcaactgcgt gcgctgcgct attttaaaga
      961 gatccctgcc ctggatccgc aactgctcga ctggctgttg ctggaggatt ccatgacaaa
     1021 acgttttgaa cagcagggaa aaacggtgag tgtgacgatg atccgcgagg ggtttgtcga
     1081 gcagaatgaa atccccgaag aactgccgct gctgccgaaa gagtctcgtt actggttacg
     1141 tgaaattttg ttatgtgccg atggtgaacc gtggcttgcc ggtcgtaccg tcgttcctgt
     1201 atcaacatta agcgggccgg aactagcgtt acaaaaattg ggtaaaacgc cgttaggacg
     1261 ctatctgttc acatcatcga cactaacccg ggattttatc gagataggcc gtgatgccgg
     1321 actgtggggg cgacgttccc gcctgcgatt aagcggtaaa ccgctgttgc taacagaact
     1381 gtttttaccg gcgtcaccgt tgtactaaga ggaaaaaaat atggagtgga gtctgacgca
     1441 gaataagctg ctggcgtttc atcgcttaat gcgtacggat aagccaattg gcgcgttact
     1501 gctgctctgg ccaacattat gggcgttgtg ggtggcgaca ccgggcgttc ctcagctctg
     1561 gatcatggcg gtgtttgtcg cgggtgtctg gctgatgcgc gctgctggtt gcgtggtgaa
     1621 tgattatgct gatcgcaagt ttgatggtca tgttaagcgc acggcgaacc gaccacttcc
     1681 cagcggcgcg gtaacagaga aagaggcgcg cgcgctgttt gtagtgctgg tactgatttc
     1741 gtttttactg gttctgacgc tgaatacgat gaccattctg ttgtcgattg ccgctctggc
     1801 attggcgtgg gtgtacccgt ttatgaagcg gtatacccat ctcccgcaag tggtgctggg
     1861 cgcggcgttt ggctggtcga ttccaatggc ttttgccgct gtgagtgagt cggtgccatt
     1921 gagttgctgg ttaatgttcc tcgccaatat tctctgggcg gtggcttacg acacgcagta
     1981 tgcgatggtt gaccgcgatg atgatgtgaa gattggcatt aaatccacgg caatcctgtt
     2041 cggccaatac gataaattga ttattggtat tttgcagatt ggcgtactgg cactgatggc
     2101 gatcatcggt gagttaaatg gcttaggctg gggatattac tggtcaattg tggtggctgg
     2161 cgcgctgttt gtttatcaac aaaaactgat tgccaaccgc gagcgtgaag cctgctttaa
     2221 agcatttatg aataataact atgttggtct ggtactgttt ttaggactgg caatgagtta
     2281 ctggcatttc tgatgatgta aaaaagccgg atgatcatcc ggctttcttc tgggttgcct
     2341 gatgcgttgc gcttctcagg cctacccggt gaatcgcaat ttattgaatt tgcaggtcat
     2401 ggtaggccgg ataaggcgtt ttcgccgcat ccggcaattc tctctgatta cccttcgccc
     2461 tgcgtcgcac tctcaatcgt caaacgcacg tctgatgtaa tcaactccgc cagcaactga
     2521 taaaccttca tcgtttctgc tggttcggca tcgccgctat cgctgatata cccttcatca
     2581 cgcagcgtca gcaccagaga actgaacacc gccttgtcga agaactccgg cgcgttgatg
     2641 ccgtgcagca cggagagacg ttgcgcaacg gtgcggctct ctttctccag cgtaccgcgg
     2701 ttgatcgacg ggttggcact caacaaccag aaggtgatgg cataacgttg cagcgtttcg
     2761 cgcgcgcctg cggccagcag ctgtagggtg cgagaatgcg ccgggttgat atgcaactca
     2821 tcatcttgca gggtaatcag cccctgacgt tgcatctcat ttgccagcgc atcaataacg
     2881 tccggcaact cgtcgcgatc ccagcgcagg aacagctccg ctttcagcat tgggtaaagc
     2941 acattgacgt gctccatcaa tacgtcgcgg gagatgtggc gatgctgggt gacaattgcc
     3001 gccatcagcg aaggcagcac caacatatgc gcaatgttgt tgcgatagta ggtcatcagc
     3061 accgcttgct cgcgtggcag aatgatgatg tcgccgatag tgtctttctc gacttcaaac
     3121 ttgttcattt gcagcgcgtg atcgataagc tcgctggcgc tggctgaagg aacggtagag
     3181 tccgtggagt aaggtacgtt gcgcatcaga tccaggtagc agttgagttg ctcggttaac
     3241 tgctcgcggg tgagtgagcg ctgacgtgat gccagtagcg cagtacagca caggttcatg
     3301 gcgtttgccg cgcctgcgtt gttaatgcgt accatcagat cggcagcaat attattgacc
     3361 gtcggcgtta accaggccgg acgcaccgct tcgatgggat cgatagattc acgccagtct
     3421 ggtacgtgct ggttaaggta ggtcatcaac ggcattggtt caccgaagtt gacgtaaccc
     3481 tgaccgagat tacgcagctt gcttaaaccg cgcagcatct gcggcaggct ctctttctct
     3541 ttcgtcgcgc cgcgcagttc tttggcgtaa gtacccactt ccatgacgtg ctcataaccg
     3601 atatagatcg gaatcagcgt aatcggacgc gtgccgccac gcagcatcgc ctgaatggtc
     3661 atcgacagcg taccggtttt cggatccagc aaacgccccg tgcgggaacg accgccttcc
     3721 acgaagtact cgacggaata accacggctg aacagttcgc cgagatactc acggaaaacg
     3781 gtggaataaa gtttattgcc tttaaaagta cggcgaataa agaacgcccc cagacggcgg
     3841 aaaatcggcc cggccggcca gaagttcagg ttgatcccgg cggcgatatg cggcggcacc
     3901 agtccctggt ggtacagcac gtaagaaagt agcaggtagt ccatgtgact gcggtggcaa
     3961 ggcacataca ccagctcatg accgtcgtgg gccagctggc gaacgcgctc ggcgttatgg
     4021 acgttgatgc cctgataaaa tcggttccag gtgaagccca gaatgcggtc agtcaggcgg
     4081 atcatctcgt aagagaaatt cgccgcaatc tcttccatca gcgcaatcgc gttctgctgc
     4141 gctttttcat gggagatttt tttgctgcgc gcttcatctt ctaccgcttt ggcaatggcg
     4201 cgggaggcga gcagcttatt aaacagatcc tgacgagcag gcagacgtgg gcctacggca
     4261 gccagacgtt gacgggcaaa gtgcatacgc gccacgcgcg ccagtttctg agcgatagtt
     4321 ttatccgtgc cgtgttcatc cgccatacgg cgcagcgaca ctgacggcga gaaacgcaca
     4381 aaactgtcgc gaccgagcca cagtacagcg aaaaatttct gtacgccgtt aagcatacgc
     4441 agcggcgggt tcacttcgcc tttttcacgc cccggcgcgc gaccaaacat caccgatact
     4501 ggcaccatct gcacatccag atttgggttg ctacggtgca aatcgagata gtcgtggaaa
     4561 agcttaatag actcttcttt cggcgtgtaa taggtgaata cacgcggccc gccgtgaatg
     4621 aacacatagc gcggcagtag cgtgccgtcg atttccagcg gctctaacgg gtcaggcaaa
     4681 tcatgtgcca gacactgggc gcgcaacgtc agcaaatctg ctttcgagtt gtacggtaaa
     4741 acgtacataa ttggacgaga ggtatccagc cccagttccg gggcaggatc tgccggaata
     4801 gacttgcttt ttaccaggat gcttaatggt aaattcagta atttgtagta aattcgtggc
     4861 cagccggaca taaacgatgt aaagcctctg gttaataatg caaatgcgcg gcaaggatag
     4921 cagaaagtca tgggaaattc tgtggtatcc gctcatgttt cgcgcggcgt tacgcaaacc
     4981 cgaatcatcg gatttaacgg tacactgata ttgacgctca taatgtaaaa aggttctttc
     5041 aatggccaat aatacaactg gattcacccg aattatcaaa gctgctggct attcctggaa
     5101 aggtttacgc gctgcatgga tcaacgaagc ggcattccgt caggaaggcg tagcggtatt
     5161 gttggcggtg gtcatcgcct gctggctgga tgtggacgca attacccgcg tgctgcttat
     5221 cagctccgtg atgctggtga tgattgtgga aatcctcaat agcgccatcg aagcagtggt
     5281 tgaccgaatt ggctctgaat accatgagct ttccggacgc gcaaaagata tgggatccgc
     5341 cgcggtgctg attgccatta tcgtcgccgt gattacctgg tgcatcctgt tatggtcgca
     5401 ttttggataa cccttccaga tttcgataaa tctctggttt attgtgcagt ttatggttcc
     5461 aaaatcgcct tttgctgtat atactcacag tataactgta tatacaccca gggggcggaa
     5521 tgaaagcgtt aacggccagg caacaagagg tgtttgatct catccgtgat cacatcagcc
     5581 agacaggtat gccgccgacg cgtgcggaaa tcgcgcagcg tttggggttc cgttccccaa
     5641 acgcggctga agaacatctg aaggcgctgg cacgcaaagg cgttattgaa attgtttccg
     5701 gcgcatcacg cgggattcgt ctgttgcagg aagaggaaga agggttgccg ctggtaggtc
     5761 gtgtggctgc cggtgaaccg cttctggcgc aacagcatat tgaaggtcat tatcaggtcg
     5821 atccttcctt gttcaagccg aatgctgatt tcctgctgcg cgtcagcggg atgtcgatga
     5881 aagatatcgg cattatggat ggcgacttgc tggcagtgca taaaactcag gatgtacgta
     5941 acggtcaggt cgttgtcgca cgtattgatg acgaagttac cgttaagcgc ctgaaaaaac
     6001 agggcaataa agtcgaactg ttgccagaaa atagcgagtt taaaccaatt gtcgttgacc
     6061 ttcgtcagca gagcttcacc attgaagggc tggcggttgg ggttattcgc aacggcgact
     6121 ggctgtaaca tatctctgag accgcgatgc cgcttggcgt cgcggtttgt ttttcatctc
     6181 tcttcatcag gcttgtctgc atggcattcc tcacttcatc tgataaagca ctctggcatc
     6241 tcgccttacc catgattttc tccaatatca ccgttccgtt gctgggactg gtcgatacgg
     6301 cggtaattgg tcatcttgat agtccggttt atttgggcgg cgtggcggtt ggtgcaacgg
     6361 cgaccagctt tctctttatg ctgttgctgt ttttacgcat gagcaccacc gggctgactg
     6421 cgcaggctta tggtgccaaa aatcctcagg cattagcccg tgcgctggtg caaccgttgc
     6481 tgttggcgtt gggggccggg gcgttaattg cgctgttgcg tacgccgatt atcgatctgg
     6541 cgctgcatat tgttggcggt agtgaagcgg tcctggaaca ggcgcggcgc tttcttgaaa
     6601 tccgctggtt aagcgctccg gcgtcgctgg cgaatctggt attactcggt tggttactcg
     6661 gcgtgcaata tgcccgtgcg ccagtaattt tgttagtggt cggcaatatc ctcaacattg
     6721 tgctggatgt ctggctggtg atggggctgc atatgaacgt gcagggcgcg gcgctggcga
     6781 cggttattgc ggaatatgca acattgctga ttggtctgct aatggtgcgt aaaatcctca
     6841 aactacgcgg aatttccggc gaaatgctga aaactgcctg gcgaggaaac ttccgtcgct
     6901 tgctggcgct taaccgcgat atcatgctgc gttcgctgtt gttgcaactc tgtttcggcg
     6961 cgatcaccgt acttggtgcg cgactgggga gtgacattat cgctgttaac gcggttctga
     7021 tgacgctact cacctttacc gcctatgcgc ttgatggttt tgcctacgcg gttgaagcgc
     7081 actccggtca ggcatacggt gcgcgcgacg gtagccagtt gctggatgta tggcgggcag
     7141 cgtgccgcca gtcggggatt gtagcgttac tattttcggt ggtttatttg ctggcagggg
     7201 aacacatcat tgcgttgctg acgtcgttaa cccagattca gcagctggct gaccgctatc
     7261 ttatctggca ggtgattttg ccgttggttg gcgtctggtg ttatctgctg gacggcatgt
     7321 ttataggcgc aacgcgtgcc gccgaaatgc gtaacagtat ggcggtggcc gccgcaggtt
     7381 ttgcgctgac gctccttacg ctgccgtggc tgggtaatca tggtttgtgg ctggcattaa
     7441 ccgtctttct ggcgttgcgc gggctttctc tggcggctat ctggcggcgt cactggcgca
     7501 acggtacctg gtttgccgca acgtgacggt taaaaattct gaataaatta tcctaagcca
     7561 aattgctgac tacacttaat ctcacgttca gaagaaaagt gaacgtactc tcattcacaa
     7621 cctaacgatg aggtcttgat tatgaataaa gatgaagccg gcggtaactg gaaacagttt
     7681 aaaggtaaag tgaaagagca atggggcaaa ctgaccgatg atgatatgac gatcattgaa
     7741 ggtaaacgtg atcaactggt cggtaaaatc caggaacgtt atggttatca gaaagatcag
     7801 gcagaaaaag aggtcgtgga ttgggaaacc cgcaatgaat atcgctggta attaatccct
     7861 cctgcccgac gtgtacaagg atgtacgccc tcttaacgcg gtttcttttt cacctgcaca
     7921 gagtgatcat gctggcactg ttcaggatga cgacacgctt ccacttccac gcatgccgca
     7981 cagagcccat gtgcttcaat cacattatgc cgcagggcaa accccatttt tgccgccagc
     8041 gtatgcataa tgtcttccac gccttctgca cactcttctt tcaccgcgcc gcagcgatcg
     8101 caaataaaca tggctgacgt atgcgtgggc tggtcgaaca gatgacagag cacataactg
     8161 ttggtggatt ccaccttatg cacaaaacct tgctcaagca gaaaatccag cgcgcgataa
     8221 accgttggcg gcttggcttg cggttcagct tcgcgcagta aatcaagcag atcataagcg
     8281 ctgatagcgc catcctgcag actcatcagg cgcaacactt ccaggcgctg tggggtcagg
     8341 cgcacattac gctgcgcgca gattttttca gcctgcgcta ataactcctg cgttgtggtc
     8401 ttttccatcg ggcacctcaa agtgggggtt aagaatgtcc ttactttacc atgttccagg
     8461 aaaaacaccg agaaccctta ttgttgccgt aatgttgatt ttctgttttg taggt
//
LOCUS       DAZPAB010000162         8515 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZPAB010000162 DAZPAB010000000
VERSION     DAZPAB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.162"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..417)
                     /locus_tag="ACHUPG_003215"
     CDS             complement(<1..417)
                     /locus_tag="ACHUPG_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFR0862724.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITIPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFE"
     gene            451..1173
                     /locus_tag="ACHUPG_003216"
     CDS             451..1173
                     /locus_tag="ACHUPG_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0862725.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(1170..1637)
                     /gene="rnhA"
                     /locus_tag="ACHUPG_003217"
     CDS             complement(1170..1637)
                     /gene="rnhA"
                     /locus_tag="ACHUPG_003217"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR0862726.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            1702..2433
                     /gene="dnaQ"
                     /locus_tag="ACHUPG_003218"
     CDS             1702..2433
                     /gene="dnaQ"
                     /locus_tag="ACHUPG_003218"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR0862727.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            2566..2642
                     /locus_tag="ACHUPG_003219"
     tRNA            2566..2642
                     /locus_tag="ACHUPG_003219"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2600..2602,aa:Asp,seq:gtc)
     gene            complement(2773..3819)
                     /locus_tag="ACHUPG_003220"
     CDS             complement(2773..3819)
                     /locus_tag="ACHUPG_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFR0862728.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(3833..4392)
                     /gene="tssG"
                     /locus_tag="ACHUPG_003221"
                     /pseudo
     CDS             complement(3833..4392)
                     /gene="tssG"
                     /locus_tag="ACHUPG_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(4389..6272)
                     /gene="tssF"
                     /locus_tag="ACHUPG_003222"
     CDS             complement(4389..6272)
                     /gene="tssF"
                     /locus_tag="ACHUPG_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR0862729.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(6288..6782)
                     /gene="tssE"
                     /locus_tag="ACHUPG_003223"
     CDS             complement(6288..6782)
                     /gene="tssE"
                     /locus_tag="ACHUPG_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR0862730.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(6779..7609)
                     /locus_tag="ACHUPG_003224"
                     /pseudo
     CDS             complement(6779..7609)
                     /locus_tag="ACHUPG_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
     gene            complement(7596..8510)
                     /gene="tagK"
                     /locus_tag="ACHUPG_003225"
                     /pseudo
     CDS             complement(7596..8510)
                     /gene="tagK"
                     /locus_tag="ACHUPG_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_137593242.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         1887 a         2256 c         2391 g         1981 t
ORIGIN      
        1 ttcaaacaac cgaccacacc caccagaaaa cagcgtgtcg ccgcaaaata gataaggttt
       61 actgaagtaa cagatatgtc ctaaagtgtg acccggtatg gtaattacac taaattcatg
      121 ccctaaaacg aaggcagttt cgccatcttt gactacctgt gttgttccct tatcttgtgt
      181 ctcttgtgga ccatacacta caatttgcgg aaacttttcc accagttctt ttacgccgcc
      241 aacgtgatcg tggtgatggt gggttagaaa tatggcctcc ggttgccagt tattggcggt
      301 aatggcgttt aatactggct ctgcgtctcc gggatcgaca atcaggcagc gacctgcctc
      361 atcattcaaa acccagatgt aattgtcatc aaaggcggga atactgttaa gattcataga
      421 ttacctctca gtgtaaaacg gaaggttgtg atgaaaccgg caagagtccc tcaaactgtc
      481 gtggctcctg attgctgggg cgatttgccc tggggagagc tttatcgcaa ggcgctggag
      541 cgccagctca acccgtggtt cactaaaatg tatggttttc atctgcttaa gattggcaat
      601 ttaagcgcag aaatcaattg cgaagcgtgc gcggtttctc atcaagtgaa tgtttctgcg
      661 caaggaatgc ccgtccaggt gcaggcggat ccacttcatc ttccttttgc cgataaatcc
      721 gttgatattt gtctactggc acataccttg ccgtggtgca ccgatccgca tcgtttatta
      781 cgtgaagccg atcgggtact gattgatgat ggctggctgg tgattagtgg cttcaacccc
      841 atcagtttaa tgggattacg caaacttgtg ccggtattgc gcaaaacctc gccctataat
      901 agccggatgt ttactctgat gcggcaactg gactggctct ctttgttgaa ttttgaagtg
      961 ctgcacgcca gccgtttcca cgttctcccg tggaacaaac acggaggaaa actattgaat
     1021 gcgcatattc ctgcgcttgg ttgcttacaa cttattgttg cccggaaacg gactattcct
     1081 ttaacgctaa atccgatgaa acagagtaaa aacaagccac gaattcgcca ggcggttgga
     1141 gccacccggc aatgtcgtaa accacaggct taaacttcga cttggtagcc cgtatcttcc
     1201 agtgtgggat tcatcgccgc ggcacgagcc agttcatcac agcgttcgtt ttccgggtgt
     1261 ccggcatggc ctttaaccca ttcccatttg atttgatgct gccccaatgc agcatcaaga
     1321 cgttgccaga gatcgacatt ttttactggt tttttgtctg ctgttttcca gccacgtttt
     1381 ttccagttat ggatccactg ggtgataccc tggcggacat actggctgtc ggtactcaaa
     1441 atgacttcgc aatgttcttt taacgcctcc agcgcgacaa tagcggccat caactccata
     1501 cggttgttgg tggtgcgggt gtagcctgcg ctaaaggttt tctcgcgtcc gcgatagcgt
     1561 aaaatagcgc cgtaaccccc aggtccagga ttacccagac acgaaccatc ggtgaaaatt
     1621 tctacctgtt taagcatctc tggtagactt cctgtaattg aatcgaactg taaaacgaca
     1681 agtctgacat aaatgaccgc tatgagcact gcaattacac gccagatcgt tctcgatacc
     1741 gaaaccaccg gtatgaacca gattggtgca cactatgaag gccacaagat cattgagatt
     1801 ggtgctgttg aagtagtgaa ccgtcgcctg acgggcaata acttccatgt ttatctcaaa
     1861 cccgatcggc tggtggatcc ggaagccttt ggcgtacatg gtattgctga tgaatttttg
     1921 ctcgataagc ccacgtttgc cgaagtagcc gatgagttta tggactatat ccgcggcgcg
     1981 gagttggtga tccataacgc agcgttcgat ataggcttta tggactacga gttttcgttg
     2041 cttaagcgcg atattccgaa gaccaatacc ttctgtaagg tcaccgatag ccttgcggtg
     2101 gcgaggaaaa tgtttcccgg taaacgcaac agcctcgatg cgctatgtgc ccgctacgaa
     2161 atagataaca gtaaacgaac gctgcacggg gcattactcg atgcccagat ccttgcggaa
     2221 gtttatctgg cgatgaccgg tggtcaaacg tcgatggctt ttgcgatgga aggagagaca
     2281 cagcagcaac aaggtgaagc aacaattcag cgcattgtac gtcaggcaag taagttacgc
     2341 gttgtttttg cgacagatga agagctggca gcacatgaag cccgtctcga tctggtgcag
     2401 aaaaaaggcg gaagctgcct ctggcgggca taaatacatg taaaaggcgc taaaaatagc
     2461 gacttgggcg atttttgcag caaacgattc aaaagatgag aaaaatcgtt gacgaaggtc
     2521 gaggcaatcc gtaatattcg cctcgttccc aacggaacac aacgcggagc ggtagttcag
     2581 tcggttagaa tacctgcctg tcacgcaggg ggtcgcgggt tcgagtcccg tccgttccgc
     2641 caatattcag aaagcccgaa tcagtaatga ttcgggcttt cgtcgttttg gtgaagtgag
     2701 aatttttcac tccgaagtaa aacggatatg atttaaaaaa tggccagaca ttataaattc
     2761 tggccacttc tgttactcca gcgcatccca tttctccagc agttctactg gaatttcatt
     2821 tattacctcc gagacgcgtt ttccgaccag gcgttctgcc tggcgcagca aaggaatggt
     2881 cgggctgctc ggttcgctgt gctcaaacca gcgacggatg acacgcaggc gttccagcgc
     2941 gtcgtttcgg tcgcggatct gccggggttc tgcgccggtc atcggtgata tttcctccgc
     3001 gatatgcacc ggtagcgcaa tctgctctgc ctgcatctgt tcagtatgaa cgatggtggc
     3061 gttttccggc tgctcagcct gtggttgtgg caggatctcc ggcgtggtgc tctgcactgc
     3121 tcccggtaac agggccagca gttgcgtcag acgggagaag tccggtgcct ggtcattcag
     3181 cgtttcacgc gcatggcgtt gcagccgctc agtggtctct gctgcctggc gaaaggcatc
     3241 cagcggcagt gtgccgcgcg cttccaggtc tgccagttgc tggcgtaccg attctggtgc
     3301 cagcgcatcc gccgggcgta atgcagatag cgaacgctcc acatcccgca cctgaaggcg
     3361 catggcagca ttatttgaca gtgtaatgcc acgaatatcc gccattacac cttcgtggtc
     3421 cagcaacgcc gccagcgcgt tactgcgtgc caccgcagca tcttcggcgg tgatatcttc
     3481 agtcgcgagc agttgaggat ggatggcgtc cgggtacgtg gagcacaacg tttccagcag
     3541 cgtgagcgcc tccgacagtc ccctggcacc ggcctgctgg atacggcagc gcagcaggac
     3601 aaccagaatg cggatatctt tgctgcgcat cagcagacga tgggcgtccc gctcaagctc
     3661 cgcccagtta atgttttccg gcgtactgac aaaatcgcca tattgcgctt cttcacgcgg
     3721 tgcggcgcgg gtgaacagca gcaggtattc cggatcgtac tccagatccg ggccgcaggg
     3781 gcattccgcc gaaattgggt cagacaggaa tatttccatt taattatcct cagtgcgcca
     3841 gcgtggtggc cagaccgtcc ggattgctgg actcacgcac cagatgggcc gatgccgcca
     3901 gacgagcgtg atctggcaac gggctgtcag cgctctcccg caaatcctgc ccgctgaggc
     3961 tggcgacata aaaggcaaag cggtcatgtt gcggtttatc cagaccgccc ccagcggact
     4021 gggcgctgcg ccatgcctgg taaaactgcg tcagccagcg gtgatgaaaa agatcagaaa
     4081 aacgggtcag cgtcggcttt cagtccggtt acgcgccagt tcggtgtagt gcagcggcag
     4141 tggaccgttc ggcccccaca gcacgaggct gtacagactg atgtgcaggc gcccttcctg
     4201 ccagcccaca ctggcgattt ctcgtggcgc aaaggtcagc gcgggcgttt gtccgaggcg
     4261 aaatttttcc atccgtggtt gccaggtgtt gcccgccgga acggtacaca actgcacatc
     4321 cacgcggcgc atcaggttca gaaaaccgta acgccagggg ggggcctgcg cctgagtgaa
     4381 atcgttgctc atacgcttcc ccgctgtccc gccctgacag gccaggtcat aattttgccg
     4441 cgctgcatgc tgtgcagcgt catctgcgag aaggtattaa tggaaacatg gcgggcgata
     4501 tagtgttcca gcaccaggcc gaacagatac ggactgatgc cggaaaaacc ttcttcatcc
     4561 accgtcagtt cacaactgac gccacgccca tagaccagta gtccggaacc gggcagacgg
     4621 cgggtgaccg gggtcgtttt acaaccaatc aggctgcgca cctggcgcga ttgcggactg
     4681 tcatgagcag gaataaacag gttcagcaga tctcgcaacg cctggccgcc ggtacggtga
     4741 tccagatcgg ccagcggcag ataattaaaa gataactggc ggatcaaccg ccatgccatc
     4801 tcacgttcag ccatcggggg ctgcggcgag cgtggcgggc ggataagccc tacgcccgcc
     4861 accgggatgg ctgcatccac ggttagatcg tcccggccat tgcgggcaat aagacagggc
     4921 agatcgcggt tagtgaccat ggccgtgatg gtgatgtggc gcagattttc cgggtacggc
     4981 gcttcatact ggtcaaccag cgacaggaac acttccgagc ccgtataggg ggtacgggtg
     5041 ccgtaacggc gggcgttttc tgataagcgg cggggctccc ggcgcaggga aaaataacgt
     5101 ccgtgattgc cttcatcatt attgcgggtg tggtagagcg ggcgaaaggc catcttccgt
     5161 gtggtgtcgg tttccagccc ttccacttcc tggaccgaga acacctcata atccagcgga
     5221 tgggttctgt ccaccaccag atgctgttcc gtcgtgctgt gcgtgacatc gatccgggcg
     5281 gtgacgcggg gaaacaggtt gataaccggg gtacagaaca ggctgaactg tgccgcattt
     5341 gtctggtgga tcagccagtc cggcggcagg cggttcagca ggataacaat ctccgccacg
     5401 ccaccgtcaa ttttctgtaa tccggcagac aggcctgtcg gggtgaaaaa atagaagcgt
     5461 tccggacagg caaaatattc atgcagcagg ttatgaccgt gaaacacgtt ccacgccagc
     5521 ggcagcagcc cctgatccgg ctccaggcct tcgtacataa cgggctgttg cagattcaca
     5581 tccagcgcgc cgtcaaaatg ccccggaatg ccagccaggg gcgcgatggc gctggtatgt
     5641 agaagttcta acaggtgtga ggccgtacgc tcctcgccgc agagataaaa cggcagcctg
     5701 tccagtccgg caagctgact gaacgttagt tcaccaaagg tgcgcagggt aatacgcagc
     5761 gccccggcga cgtgaatgtt ggccggcagg tagcggtgca gggcaggcat atccggtggc
     5821 gcggcggtca gtcgcgcctc ttcgatggcg agcggccaca gcgttacatc ctggctgctg
     5881 cgaaactggc aggcagtgct ttcgccttcc ggaatggcgg agaaaaaggc tgtgtcgcgc
     5941 gggacggtaa atcctttcgc cagatcgcct tcttccgtat cgggatgcaa ctgcgccacg
     6001 ctcattgagg gcgtgggtgt gacgtaattg gggctgacca cttccagcag gcgctgggtg
     6061 aaacggggga attcagcgtc aatttttagc tgcgtgcggg cggtcaggaa gctgaaggcc
     6121 tcgaccattc gctctacata cggatcggca atatccgttc cctgcatgcc cagccgggcg
     6181 gcgactttgg gatgacgggc ggcaaattcg gctcccgttt cccgcagata gctcagttcg
     6241 cggttgtagt attccagtag ccgtggatcc atcggtctct ctctgaatta aaaaaacgta
     6301 atgcgactct gctccatatc cagcgcgcta cgtacccgga atgcagtcgg atacggctgc
     6361 gtgaggatct cgccgcgaat ttcaaactgt aatgtgttat ggccactctg gcggcgctca
     6421 tcctgaagtg ggatgatttg cagagtggac gctttcagcc ggggttcaaa ccgggtgatg
     6481 gttcgcaaaa tggccctgcg gatatcgtcc cacttgtgct catacagaaa gctgcccgcc
     6541 agtggcggca ggccgtagtt aagtacggac gctgccgcgt acggataacg tgcagcatca
     6601 atatcatctt catggctgac ggtgttgagc agaaatgaca aatcccggcg gatgatctcc
     6661 ttaatctgaa ccggactgac gctgataccg tggtcgcgtt tctggtttgg ggcgtcgtca
     6721 cacaggcggt caaacagcgt gggcagcagg tgattgcctg gacgctggcg ggaagcgctc
     6781 acgcgtcgct tccttcagcg gtgttaaagg tacaggtgga caaatccagc aggctgatat
     6841 cgccgtgacc ggtgagccac accctctgac ccagcgcctg taccagtgtc tctcccggac
     6901 cgtcctgcca gaccgtttcc cggcacagac gcagggagtc ggcagccttt tcagagccgc
     6961 tgtagcgggg aaacagctag gcactgtgtg tatcgccatt gttcagggtg acgttgacgg
     7021 gtttccagat caaatcgatc agatgcgcag gccgcggcga tttcagcgaa cagatttgtg
     7081 aaaacggcag ccagatatat gctccgcccg cgatcagttc gagtaccgga ccggtacggg
     7141 agtcgctgtc gctaatccag tcaaatgcgc cgccattcca ctgcccggaa gtgtctgtta
     7201 tggcctcaag cgcgcggctg cggtgagcat cagcttcctg cgcttcacca cgggcgttac
     7261 aggcgagggc ggtcagcaga tcctccatcc aggcgggcgg cgggagaata acaccgggcc
     7321 gctgttcgcc ctgaaagcag gcgtggcggt atatctcaca gcggatcagc tcgccaaata
     7381 cctgcgcttc gcgagtataa tttgcgtcca tccgggcgca tagctggatc tgttgcagtg
     7441 cccgcgacca gtcgcctgcc acgcacaata actgaaacag gctgtggcgg cgggaggcct
     7501 gcgctgggtg catcctgacc tgttgctctg ccatctgaat gccctccacc acggaataat
     7561 cccgcagcag cgccgacaag gtaatcggta gctcgttatt gtttgtcatg atatatctct
     7621 ccatgttgtg ccgggtttat ccggtaatgg ctgtcaatgc caatgatgcg atgctctttc
     7681 cgtgtcagat ctggcagtac ggtgttatgc gctgtttccg gggcgtattc cggggaaagt
     7741 aaatgcagga tatcaggggg agtcttttcg acaggccaga tagtttcatc ttcgccagtg
     7801 gcatccagta cattgagaat agcgtcaatg cttaacccac cagtgaccat atcctgcaat
     7861 gtctcggctt cggtgaccag gtcacgcaat gaatccagat cctgcgagga gggctcttcg
     7921 ttaaaaggaa aaggatcagc atggcgatta agatcttgtt cgggagataa caacgcctcg
     7981 cggtattcct gataaagtcg ggtcagggca tttccttttt ccgcaccttc tccggtggag
     8041 gaggctccat gaacaggaat gatatcgaat ggattttgtg atgtcagatg ctgctgcctg
     8101 aaccaatcca gatccagata ttcggttatt gcttccgacg tgatggcagt ctgcgatatc
     8161 gtttttactg tgctcaggtt cggagatgat ggattgccat gtacaaggca ctatgaagat
     8221 aacccccatt ctatcaggtc gccttcattc agacgcatac ggtgatgagg ttccacaatc
     8281 tggtcgttta ccgtacagca atattcggta gactgattga taatccacca tgcttcttcg
     8341 tggcgggtca gagtgagggc aatctttaca tcatcattca ccttaaaaac aggaatccag
     8401 ggagaggtaa cggtgaagat aatttgtgtt ccttcagtcg cgtaaattgt gttatcggcg
     8461 atcccgtgcg cctgtatttt ttggagtccc cacgtaatat ttggtttcat tgtgt
//
LOCUS       DAZPAB010000163         8495 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZPAB010000163 DAZPAB010000000
VERSION     DAZPAB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.163"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            44..397
                     /locus_tag="ACHUPG_003226"
     CDS             44..397
                     /locus_tag="ACHUPG_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR0862731.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(434..2512)
                     /gene="flhA"
                     /locus_tag="ACHUPG_003227"
     CDS             complement(434..2512)
                     /gene="flhA"
                     /locus_tag="ACHUPG_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFR0862732.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="ACHUPG_003228"
     CDS             complement(2586..3596)
                     /gene="hypE"
                     /locus_tag="ACHUPG_003228"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR0862733.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="ACHUPG_003229"
     CDS             complement(3593..4714)
                     /gene="hypD"
                     /locus_tag="ACHUPG_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR0862734.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="ACHUPG_003230"
     CDS             complement(4714..4986)
                     /gene="hypC"
                     /locus_tag="ACHUPG_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR0862735.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="ACHUPG_003231"
     CDS             complement(4977..5849)
                     /gene="hypB"
                     /locus_tag="ACHUPG_003231"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR0862736.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="ACHUPG_003232"
     CDS             complement(5853..6203)
                     /gene="hypA"
                     /locus_tag="ACHUPG_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR0862737.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            6415..6876
                     /gene="hycA"
                     /locus_tag="ACHUPG_003233"
     CDS             6415..6876
                     /gene="hycA"
                     /locus_tag="ACHUPG_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR0862738.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            7001..7612
                     /gene="hycB"
                     /locus_tag="ACHUPG_003234"
     CDS             7001..7612
                     /gene="hycB"
                     /locus_tag="ACHUPG_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR0862739.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            7609..8495
                     /locus_tag="ACHUPG_003235"
                     /pseudo
     CDS             7609..8495
                     /locus_tag="ACHUPG_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
BASE COUNT         1904 a         2307 c         2287 g         1997 t
ORIGIN      
        1 attaagatgt aatataaatg catcttaatg aggtggaggc agcatgtccg gcaagcgtat
       61 ctctcgtgaa aaactgacga ttaaaaaaat gatcgatctt tatcaagcga aatgcccgca
      121 ggcgtcagcg gagccggagc attacgaggc gttgtttgtt tacgcgcaaa agcggctgga
      181 taaatgtgtg ttcggcgaag agaaaccagc ctgtaagcag tgtccggtgc actgttacca
      241 gcctgcaaag cgtgaagaga tgaaacagat tatgcgttgg gccgggccga gaatgctgtg
      301 gcgtcatccg atcttaaccg tgcgtcatct catcgatgac aagcgtccgg tgccagaact
      361 gccggaaaaa tatcgtccga aaaaacctcg tgaatagtat gccggacagg cagatctgtc
      421 cggcaatttg cagttaaatc aatgccgatt tatcaattcc cagccgcttc atccgtgaca
      481 gcagggtcgt gcgtttcagc cccagacgtt gcgcagcgcc tttaggcccg gcaaccacgc
      541 tgttggtttc tttcagcacg cgcacaatca actgatattc atcttcgccc tcctgggcga
      601 caaccgttgc ggcaggcggc gtttcaggct ccggtaaagc aatatctggc aatgacagct
      661 gcagcacgtt gccgcgtgtt agcaatactg cgcgctcaat gacgttttcc agttcgcgta
      721 cgttacccgg ccactccatg ttgctcaagg tgcgcagcgt ctcggcagga atgctgtcga
      781 tattgcgccc cagacgacgg gcaattttga aggtaaaggc tttcgccagc agcggaatat
      841 cttccggacg ctcgcgtagt ggcggcaggt gaatcgggaa tacgttcagg cggtaataga
      901 gatcgctacg gaactctcgg tcggcgacca tttttttcag atcgcggtta gtcgcggcaa
      961 ttaaacgtac gtccgtctga atgattttgt tgctgccgag acgttcaaac tcctgttcct
     1021 gcaatacacg cagcaacttc ggctgtaact ccagtggcat atcgcccact tcgtcgagga
     1081 acagagagct tttatccgcc agttcaaaac gaccaatgcg ctgggcgttg gcaccggtaa
     1141 aagccccacg ctcatgacca aacagatcgc tttccagcaa tccggcaggc atcgccgcgc
     1201 agttcatttt gaccatgcgg cgattattac gcccactgag attatggatc gcacgggcaa
     1261 tcagctcttt acccgagcca gtttcaccga ggatcagcac ggtactgtca ctttgcgcca
     1321 ccatttcaac ttgtttaagc acgctgtaca tggcttcgct gcggccaata atctcgccaa
     1381 attcactatc aacattgttg agctgctcgg tcagggcgag gttttcatca accagccgtt
     1441 ctttcagacg atggatttcc tgataggcga gggcgttatc gacagcgatt gccacacgtt
     1501 cggcaatctg gcgcagcaaa ttcagattgg tagtggtaaa cactttctct tcgcattgcg
     1561 ccagtttcag cacgcccagc atggtgtcgc cagacatcag cggtaacagg cacaaggttt
     1621 gaatctggtt gccccaggtg tcgaacaaca tgcgttcata gggggctaaa tcgtcccgct
     1681 cgtagagatt gatcagcagc atctctttac ttttgaacac gcgttcggtg agggttccgg
     1741 cttcatcgac ttcgctctgt tcgtgggcgg gatgttgttt atcaagatag tgagtggagt
     1801 agatgttgag tttgttttta cggtggctgc gtaagacaat gctgatatcg tcaatgtcga
     1861 aatagtaatg gatttctttg gcgacttcgc tgaccagttc gtccatatcc aggcgggaaa
     1921 gcaccgcgtt ggtaatggca accaggatgc ggaagttatc gcgctcccgg cataacaact
     1981 catagtcgac attgttgacg acgcggctct ggatttgttc ggtgacgaca gaaacgatct
     2041 gcgtaaatgt ttgcagacga ttgaactctt tttcgctcca gggacgctcg tcataacgaa
     2101 taaattcaca gccaccaaaa atatgccctc ccgccgccag tggcatcagg caatagtgac
     2161 caaatttggg gtatagccca cctgcggcca gctgcggcca ggtttcacaa aattcttcgt
     2221 aactgcaatg cagcgtatca gggcgcgaca aaatgctgcg taccggaccg tgtgccagaa
     2281 cagtttcgtc ttcatattta atgggggtgt ctttttcacg cgacgcgtaa taagacgcac
     2341 gttgagtctg cgcttgccac aacacaatag ccgcgttgtc ggcgagcgca gaacgcttta
     2401 ccagttgcga aagagcctca cacagcgagg ccagatcggg ctgctgcaat agtgtccgag
     2461 tgatgtcgaa caacccttgc tgtccgagat cactcatcgg tgtatatgac attttgttct
     2521 tccaggaaag caccgcaaag cggtgcaaaa ggtcattgac taactatagg agatttagaa
     2581 ttttattagc atatacgcgg aagcggttcg gcgtgtggta aatcgagggt tcgtttcacg
     2641 ccatacagac cggcaagacg aacaccttta cgttccacca cttcaccaat cagcgccgcg
     2701 tctttcccca gtggatggga atgtaacgct gccagcactt gctctgccgc gttgcgttca
     2761 acggcgatca ccagtttgcc ttcgttggca aagttcaggg cgtccagtcc cagcaattcg
     2821 caaacgccgc gcacggcagg tttaaccggc agcgctgatt cagaaatttc aataccacaa
     2881 ccgcaggctg ccgcgaactc atgaaccacc gcgtttacac caccacgggt ggcatcacgc
     2941 agcgctttca cgccgggaat gtcacgcagc gtctgaataa gcggcgtcag caccgcgcag
     3001 tcgctgacca gttcgccatc cagccccagc tgctcacgca ggttaaggat agtcgccccg
     3061 tggtcgccga gtgtaccgct aaccagcaat acatcgcctg cggttagcgt ctgtgcgccc
     3121 cagtgaatat tcgccggaat tgcgcccatg ccagcggtgt tgataaacag tttatctgcc
     3181 gcgccgcgct gcaccacttt agtatcgcca gtaacgatgg caatgcctgc cgtgcgggcg
     3241 gtttctgcca tgctggtcac tacggctttc agtgtctcca tcggcaatcc ttcttcgagg
     3301 ataaagccac aggagagata gcgcggaata gcgccactgc ccgcaacgtc attcgcggtg
     3361 ccgcaaatcg ccagcttgcc gatattaccg ccaggaaaga acagcgggtc aatgacgtaa
     3421 ctgtcggtgg agaacgccag acggtcgcct tccgctacca gctgcgccag atcaagacgt
     3481 gcctgatctt cctgctctgc cagccacggg ttggcaaagg cttccataaa caggctgttg
     3541 attaattgct gcatcgcctg gccgccgctg ccgtgggcga gttggatatt attcacgctt
     3601 cactctcctg ctgacgatac tgataccacg cggcgcacgc tccttcggag gagaccatca
     3661 gtgcaccaaa tgcggtttga ggattacagg tgttaccaaa cagcgggcat tgatgcggct
     3721 tacatttgcc cgttaatacc tcaccacaac gcgcgcgcgg gtcatcgcag acctgctgcg
     3781 gtgccgggcg gaaatgtgct tcggcatcga atcgttgata atccggcgtc aggtgcacgc
     3841 cagaagattc aatcacgcct aagccgcgcc attcgctgtc gccgttgaca cagaacacat
     3901 cggcaatcgc ctgttgcgcc agtaggttac cggcatccgg taccacccga cgatactgat
     3961 tctctacctt gctgtgggcc gctattttct gctccaccag catgaccacg ccttgcagta
     4021 gatcaagggg ttcgaaacca gcgaccacca gcggacgatg aaaatcgctg gcgataaaat
     4081 tataggcatc agtgccgata accatactaa cgtggcccgg cgcgaggaac gcgtcgatac
     4141 cgttatccgg ctgttccagc aaactgcgca gcgtcgggat aagcgtaatg tgctggcaga
     4201 agaagtaaaa attctgcaca tcacgcgctt tcgcctgttg cagagtgatg gcggtggtcg
     4261 gcatggtggt ttcaaaacct aagccgaaga acaccacttt gcgggttgga ttctcctgcg
     4321 ccagtttcaa cgcatccatc ggcgagtaaa cgatgcgcac atcggcaccg cgtgcttttg
     4381 cctgcaacag cgatccctgt ttccctggta cgcgcatggc gtcgccaaag gtacagaaga
     4441 tgacttccgg atggctggca atctccacgc aggtgtcgat tctacccatc ggcagtacgc
     4501 acaccgggca acccggaccg tggataaact caacgttttc aggcagtaac tggtcgaggc
     4561 cgaatttaaa aatggcgtgg gtatgaccgc cacacacttc cataatccgc agagggcgtt
     4621 cggcggtgta agagagatgt gaagcacgtt cgcgcagatg ctcaattaac tgcatcacct
     4681 gttccggcgc gcgatattca tcaacaaaac gcattatttt tcctcgccat acaacagcgc
     4741 gccgacatcc ggctcaacgt caaacatgtt ttgtaaggcg tcgagagtgt cgcgtgcttc
     4801 ggcttcatta attacgctca tggcaaagcc aacgtgaacc agtacccact ggcccacgcg
     4861 cggctgaccg ttttcatcgc agctgccgac taacgttaaa tcgacatcgc gctgaatgcc
     4921 gcagacgtcg actttcgcct ggttgccgtc aatggtgcgg atctggccgg gaacgcctat
     4981 gcacatcgct gtgtctccag ccagttcagc cactggtcca tcccttcgcc gctggtggcg
     5041 gaaataagga tgatttcaat ttctggattg acttcgcggg cgcaggcgat gcacttctca
     5101 acgtcaaagt tgagatacgg caacaggtca actttgttga gcagcatcag cgaggcggcg
     5161 gcaaacatat gcggatattt cagcggtttg tcttcacctt cggtaacgga aagcaccgct
     5221 actttgtgtt tttcaccgag atcgaagctg gccgggcata cgaggttgcc aacgttttcg
     5281 ataaacagaa taccgttatc gtccagtggc agacgcggtg cggcgtcggc aatcatctgt
     5341 gcgtcaagat ggcagccttt accggtgttc acctgaatcg ctggtgtacc ggtagcgcga
     5401 atgcgtgcgg catcgttcac ggtttgctgg tcgccttcaa taaccgcgca cggaacgctg
     5461 tctttcaggc gcattaaggt ttccgtcagc agggtggttt taccggaacc agggctggaa
     5521 accaggttga gcaccagttg cttgcgggca gcaaagcgcg cgcggttgcg ttcagccaga
     5581 cggttgtttt tgtccagcac gtcaatttcg acttccagca tccgacgctg gctcatgccc
     5641 ggtgtgtgag tgcccgcttc gccatgaccg taatgcaggt cgccttcttc agtctggctg
     5701 ggggtaaatt caggcgcttt aatgccggtg attttcatct gcgggcgtgc cgccggggca
     5761 aatggcgcgc tacgaaacgc ggaatgaggg ttatgttcat caccctcgat atacaggttg
     5821 ccttcaccgc aaccgcatgt tgtacacatc gctcactcct ggtctatttc tatccgccga
     5881 atctgtaaac cgtcgtctgc cacaatctgt agcatgtcac catgacactg tggacagcgg
     5941 cggacgcgct gggtcagtag cgtcacatac tgttggcatg tttcacacca gcattcggcc
     6001 tcttgttctt cgaggtgcag tttacaacct tccgccacgc tgccgcggca aaccagatca
     6061 aaacaaaagg caagagagct ggtttcgaca caagaaaatg cgccaatttt gagccagacc
     6121 ccagttacgc gttttgcgcc gtgttttgcg gcctgctgtt cgatcaattc cagtgcccgt
     6181 tggcagaggg ttatttcgtg catatcgcct cccattaact attgccagct acaagcaata
     6241 attgcgccag tgttgattat ccctgcggtg aataatgtcg atgatgtcga aatgacacgt
     6301 cgacacggcg acgaaattca tctttagctt aaaaatctct ttaataacaa taaattaaaa
     6361 gttggcacaa aaaatgctta aagctggcat ctctgttaaa cgagtaacct gacaatgact
     6421 atttgggaaa taagcgagaa agccgattac atcgcacagc ggcatcgtcg cctacaggac
     6481 cagtggcaca tctactgcaa ttcgctggtt caggggatca cgttatcgaa agcgcgcctg
     6541 catcacgcca tgagctgcgc gccggacaaa gaactctgtt tcgtcctttt tgaacatttt
     6601 cgtatttacg tcaccctggc ggatggcttt aacagccaca ccatcgagta ttacgtcgaa
     6661 acaaaagatg gcgaagacaa acagcggatt gcgcaggcgc aactgagcat tgacggcatg
     6721 attgatggca aggtcaacat ccgcgatcgc gaacaggttc tggaacacta tctcgaaaaa
     6781 atcgctggcg tttacgacag cttatacacc gccattgaaa acaatgtgcc ggtgaattta
     6841 agccaactgg taaagggaca aagcccggca gcatgagctg aggctttgcc cgttttgcag
     6901 gcgttacgcc tgtttgggga tgggcgtgtc gatgagtgtc gaaaatgaca tttcatcggc
     6961 atgttttcgt caaaaatgac aatcacctga ggaatgcctg gtgaatcgtt ttgtaattgc
     7021 cgactccacg ctctgtatcg gctgccacac ctgtgaggcc gcctgttcag agacgcatcg
     7081 ccagcacggc ctgcaatcaa tgccgcgcct gagagtgatg ctcaatgaaa aagaatctgc
     7141 gccgcagctc tgtcaccact gtgaagatgc accctgcgcg gtggtctgcc cggttaacgc
     7201 catcacccgc gttgatgggg ccgtgcagtt gaatgaaagt ctgtgcgtaa gctgcaaact
     7261 gtgcggcatc gcctgcccgt ttggcgcaat tgaattttcc ggcagccgtc cgctggatat
     7321 tccggcaaac gccaataccc cgaaagcgcc accggcaccg cctgctccgg cgcgtgtcag
     7381 cacattgctt gactgggtgc caggtattcg cgcgatcgcc gtcaaatgtg acctttgtag
     7441 ctttgatgaa caaggtccgg cctgtgtgcg gatgtgcccg actaaagccc tgcatctggt
     7501 ggataacacc gatatcgccc gcgtcagcaa acgtaagcgt gagctgacct ttaacacgga
     7561 ctttggcgat ctcaccttgt ttcagcaggc tcaaagtgga gaggctaaat gagcgcaatt
     7621 tccctgatca atagcggcgt ggcatggttt gtcgccgccg ctgttctggc atttctcttt
     7681 tcaaaaagcg ctaagtggct ggatagccgg aattggcggc gcggttggta gtctgtatac
     7741 ggcagccgcg ggcttcactg tactgactgg cgcggttggc gtgagcggtg cgctgtcgct
     7801 ggtaagctac gatgtgcaaa tctccccgct taacgcgatt tggctgatta ccctcggcct
     7861 ttgcggtctg tttgtcagcc tctataacat tgactggcat cgccacgcgc aggtgaagtg
     7921 caacggcttg cagatcaata tgttgatggc tgccgccgtc tgcgccgtca tcgccagcaa
     7981 cctcggcatg ttcgtggtaa tggccgaagt catggccttg tgtgcggtgt tcctcaccag
     8041 caacagcaaa gagggcaaac tgtggtttgc gctgaggcgt cttggcactc tgctgctggt
     8101 gattgcttgc tggctgctgt ggcagcgtta tggcacgctg gatctgcgcc tgctggatat
     8161 gcgtatgcaa cagctgccgc tcggttccga tatctggctg ctcggtgtga ttggctttgg
     8221 cctgctggct gggattattc cgctgcacgg ctgggtgccg caggcacatg ctaacgcctc
     8281 tgcgccagct gccgcgttgt tttctacggt agtcatgaaa attggcctgc tgggcatgtt
     8341 aaccctgtca ctgctgggcg gtaatgcacc gctgtggtgg gggatcgcgc tgctggtgct
     8401 cggcatgatc accgcgttcg tcggtggtct gtatgcgttg atggaacaca acatccagcg
     8461 cctgctggct taccacaccc tggaaaatat cggca
//
LOCUS       DAZPAB010000164         8483 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZPAB010000164 DAZPAB010000000
VERSION     DAZPAB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.164"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            182..3049
                     /locus_tag="ACHUPG_003236"
     CDS             182..3049
                     /locus_tag="ACHUPG_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0862740.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(3149..5068)
                     /gene="dhaR"
                     /locus_tag="ACHUPG_003237"
     CDS             complement(3149..5068)
                     /gene="dhaR"
                     /locus_tag="ACHUPG_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFR0862741.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            5296..6366
                     /gene="dhaK"
                     /locus_tag="ACHUPG_003238"
     CDS             5296..6366
                     /gene="dhaK"
                     /locus_tag="ACHUPG_003238"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFR0862742.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6377..7009
                     /gene="dhaL"
                     /locus_tag="ACHUPG_003239"
     CDS             6377..7009
                     /gene="dhaL"
                     /locus_tag="ACHUPG_003239"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFR0862743.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            7020..8438
                     /gene="dhaM"
                     /locus_tag="ACHUPG_003240"
     CDS             7020..8438
                     /gene="dhaM"
                     /locus_tag="ACHUPG_003240"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFR0862744.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
BASE COUNT         2199 a         2134 c         2130 g         2020 t
ORIGIN      
        1 gggttaaata tatattaatt cctgtgttcc tgattttgcc cggtaataga gaattattct
       61 cagagaagct catcatgatt gcaccataca gatatcatga tgataagagg atattattca
      121 ttgacttaaa caaaatgaca aaatccatag acccatagcc tgggtactat ataggagtgg
      181 tatgggcatc aaacaacaca atgggaatac caaagccgat cgtctcgctg aattaaaaat
      241 ccgttcgccc tcaattcaac tgataaaatt tggcgctatt ggtttgaatg caattatctt
      301 ttcccccctg ctgatagctg ctgatacagg aagtcaatat ggcaccaata ttactattaa
      361 tgatggtgac agaattacag gagataccgc cgatccatca ggaaacctct atggtgtaat
      421 gaccccagca ggaaacacgc ctggcaatat caacctgggt aatgatgtca ccgtcaatgt
      481 caacgacgcc tctggatatg caaaaggaat cattattcag ggcaaaaaca gctccctgac
      541 agctaaccga ctcacagtag atgttgttgg tcaaacctct gccatcggca ttaacttaat
      601 tggtgactat acccatgctg acttaggcac aggcagcacc attaagagta acgatgacgg
      661 catcattatt gggcatagct caacactaac agccactcaa ttcaccattg aaaactcgaa
      721 cggtataggc ctaaccatca atgactatgg caccagtgtc gatcttggaa gcggaagtaa
      781 aatcaagacc gatggaagta caggtgttta tatcggtggt ctcaacggca ataacgccaa
      841 tggtgctgcg cgttttacgg cgacagacct gacaatcgat gttcagggct acagcgccat
      901 ggggataaac gtacagaaaa actctgttgt cgatctcgga acaaacagta ccattaaaac
      961 caatggcgat aatgctcacg gcctctggag ctttggccag gttagcgcga atgcactcac
     1021 tgttgatgta actggagccg cggccaatgg cgtcgaagtt cgtggtggta caaccactat
     1081 cggtgcagat agccatattt cttccgcgca gggcggtggc ctcgtcacca gtagttcaga
     1141 cgcgacaatc aatttttctg gcacggcagc gcaacgaaac agcatctttt ccggcggttc
     1201 ttatggtgcc tcggcccaga cggcaacggc tgttatcaac atgcaaaata ccgatattac
     1261 ggttgatcgt aatggcagtc tggcgctggg tttgtgggcg ctcagcggcg gtagaataac
     1321 cggagacagt ttggctatca ccggcgcggc aggagccaga gggatttatg ccatgaccaa
     1381 cagccagatc gacctcacga gcgatctggt cattgatatg agtacacccg accagatggc
     1441 catcgcaacg caacatgacg atggttatgc cgccagccgc atcaacgcct cgggtcgtat
     1501 gcttatcaac ggtagcgttc tttccaaagg tgggctaatc aatctggata tgcaccctgg
     1561 gtcggtttgg acaggttcct ccctcagcga taatgtcaat ggcgggaaac tggacgttgc
     1621 aatgaataac agcgtctgga acgtaacaag taattctaat ctcgacacgc tggcgctgag
     1681 ccattcaact gtcgattttg ccagccacgg gtcaactgcc ggcacattta ccacattaaa
     1741 cgtagagaac ctgagcggta acagtacctt tattatgcgt gctgatgttg ttggcgaggg
     1801 taatggcgtt aataatagag gggatttatt gaatatcagc gggagtagtg ctggtaatca
     1861 cgtattggct atccgcaacc agggcagcga ggccacaacg ggaaatgaag ttctgacagt
     1921 ggtaaaaacc actgacggcg cggcctcgtt cagcgcgtct tctcaggttg agttgggggg
     1981 atatctgtac gatgtgcgta aaaatggcac taactgggag ctttacgctt ccgggacagt
     2041 tccggaaccg actcctaatc ctgaacccac accagctccc gctcagcctc ccatagtcaa
     2101 ccccgatcct acgcctgaac ccgctcccac gcctaaaccc accacgaccg cagatgctgg
     2161 cggcaattat ctcaatgtcg gttacttatt gaactatgtt gaaaaccgta cgctgatgca
     2221 acggatgggt gacttgcgaa atcagagtaa agacggtaat atctggttgc gcagttatgg
     2281 gggaagcctg gactcctttg ccagtggcaa actgagcggc tttgacatgg gttacagcgg
     2341 tatccagttt ggtggggata aacgtctctc tgatgtaatg ccgttgtatg tcggtctgta
     2401 tattgactca acacatgcat cgccggacta tagcggaggc gacggtaccg cacgttcaga
     2461 ctacatggga atgtacgcca gttacatggc acaaaacggt ttttacagcg atctcgttat
     2521 aaaagcatcg cgccagaaaa atagtttcca cgtactggac agtcagaaca acggcgttaa
     2581 cgccaacggc actgcgaatg gaatgagcat ctccctggaa gccgggcaga ggttcaacct
     2641 gtcccctact ggttatgggt tctatataga gccgcaaacc cagcttacat acagccacca
     2701 gaatgagatg gctatgaagg cgagtaatgg cctcaatata catctgaatc actacgaatc
     2761 gctgctgggg cgtgccagca tgatactggg gtatgacatc accgcaggca acagccagct
     2821 gaatgtctat gtgaagactg gcgctatccg cgagttttca ggggataccg aatatctgtt
     2881 gaacgactcc cgggagaagt acagtttcaa aggtaatggc tggaataacg gcgtgggagt
     2941 cagtgcacag tataacaaac agcacacatt ctatctcgaa gcggattaca cgcagggtaa
     3001 cctctttgat cagaagcaag tcaacggagg atatcgcttc agcttttaag tgcgggatag
     3061 acaagccctt aaacgatgct gaaaattgtt agcgtgcgcc ggaatgaaat acagatcact
     3121 ctgatgtatc agaaatcgtt tctgtctttc atacccggcg cttaaactgc cctgcatcaa
     3181 tgccatgttg cttcattttc cgccacagcg tagtgcggcc gatcccaagt aaagccgaca
     3241 tttcctgaat gcgaccgcct gtgacctggg ctgcgttaat aattgcctct ttttcaactt
     3301 ccgcaaatga cagactggtg gaaaggcggg tggcgctgac atcatctgtc gcctgctcgg
     3361 taaacagatg ttcaggtaaa tcactgacgc gaatgcgccc gttatcacta ctcagagcaa
     3421 gattctcgat gacgctgtaa agttcaaaat cgttgcctgg ccatgcacaa gaaactaggc
     3481 gagcgagggc atcgtcatca attttcagcc gcgtagagaa gcgtttttca agactgcgta
     3541 atttgttatt caccagcgcc ggaatgctgc cacgccgcat acgcagaggc gggatggtaa
     3601 tttcaaatgc atgcagcgcg taatacagct ggcgactaaa acgattttgt tccaccagca
     3661 ttgcgaggtc cgcggtcgtt gttgcaatca ctttgacatc aattggtatt aaacgccgcg
     3721 catccagtcg cgtgataacc ccctgcttga taacctgcag caaagcagac tgtaactcca
     3781 ccgccagata ttcaatcttt tcaagaaaca gcgtgccgcc gtgtgccagt tccagccgac
     3841 tcagacggcc attttcattg tccgtgcgat cgccaccaat aaattcttcc gccagcgcag
     3901 catcaccata taactcacaa ttgacggcga tataaggacc tgcagcacgc tcgctttcat
     3961 tatgaattgc ctgacttagc agtgccttgc ccacgccctc ttctccacaa agcaggacag
     4021 gaaagctact gcgcgccgcc tggcgaccaa aatgaatcaa gcggcgggtt tgcggatcgt
     4081 cctgtggcat atgagcgaag gtatggctga cttttcctaa ttgactggtc attaactgcc
     4141 gcatctgttc cacaggatgg agcaacaaaa taaagctggt tccctgcgtt tctattatcg
     4201 gtttaagggt tatcaccgca tcaataaact ggtgctggct ttcaaaggtt gcttctacgt
     4261 gtttgagcgg atgtgccagt tttattgctt gttgcaatac ggcgggtaac gttaagagtt
     4321 cagtgattgc ccgtccctga cttgccgtcg cgtcaaggcg caagacccgc gccgcctggg
     4381 cattaataaa ttgcaaatta ccctgctcgt cccagctaat cacgccatca tccatacttt
     4441 ctaacagggc attaagttga tttaaatgac ggttagtttc agcgagcaaa ctgtccgtca
     4501 gcagtaaatt accgacctcg cgggcgattg ccaacgtcaa cggcaaatca gctgcggtag
     4561 tttgctcaac cggacacgcc agcgctattg ttcccgtcaa tcggcccttg ctgtcaaaca
     4621 acggcgttgc acaaaaggcc cagttccaga gtgcctgttt gaaatgttga tcggccatcg
     4681 ttttcacggc ctgaccagag atagccgcta acgatagcgc acaagtacca ataattccct
     4741 cggcgcaata cgtgccgtca ttgaatccca gtgcacttag ctgctgcaag gtttgcggat
     4801 cgccattacg gctgagaatg caggcggttt catcgaggat aaacagcgca cactctcgcg
     4861 gtgccatata ttcccaggca tcttccagcg cagcctgccc gagcgtcagc atcgctttct
     4921 tacgccgata aatagaagca aatgtcacgc cctgggcctg atgtggtacg ttccatgtct
     4981 cccgtttcat cagcttattg catcgctccc aggaggttgc aattaaggga gatatgcccc
     5041 gaccatcgtt gttaaaagcg ccactcatat cccgcatccc ttatgacaaa aggtgttccg
     5101 gctcgcaatt ttaagtatta atacacgtaa aagtttcaat atggaacatt cattaaagca
     5161 tatgtttcac tatgaaacat aatttgggct aattttcttt tcccttgcct gatgcacaat
     5221 ggattcatcg gcagtcagtg gtcgccgtgt cgttgaacat catccatgcc ctaccgtaat
     5281 tgctggagca aaataatgaa aaaattgatc aatgatgtgc aagacgtact ggacgaacaa
     5341 ctggcaggac tggcgaaagc gcatccatcg ctgacactgc atcaggatcc ggtgtatgtc
     5401 acccgagctg atgcccctgt tgcaggaaaa gtcgccctgc tgtcgggtgg cggcagcgga
     5461 cacgagccga tgcactgtgg ttatatcggt caggggatgc tttcgggggc ctgtccgggc
     5521 gaaattttca cctcaccgac gcccgataaa atctttgaat gctccatgca aattgatggc
     5581 ggcgaaggtg tactgttgat tatcaaaaat tacaccggcg atattcttaa ctttgaaaca
     5641 gcgaccgagt tactgcacga tagcggcgta aaagtgacca ctgtggtcat tgatgacgac
     5701 gttgcggtaa aagacagtct ttataccgcc gggcgacgcg gcgttgccaa caccgtatta
     5761 attgaaaaac tcgtaggcgc agcggcggag cgtggcgact cactggacgc ctgtgcggaa
     5821 ctggggcgta agttgaataa tcaaggccac tcaataggta tcgctctcgg tgcctgtacc
     5881 gttcctgccg cgggcaaacc ttcttttacc cttgcggata atgagatgga gtttggcgta
     5941 ggcattcatg gtgagccagg tattgaccgc cgccctttct cttcccttga tcaaaccgtc
     6001 gatgaaatgt tcgacaccct gctggaacat ggctcatacc atcgcacttt gcgtttctgg
     6061 gattatcaac aaggcagctg gcaggaagaa ccacaaacca aacaaccgct ccagtctggc
     6121 gatcgggtga ttgcactggt taacaatctt ggcgcaactc cgctttctga gctgtacggc
     6181 gtctataacc gcctgaccac acgttgccag caagcgggat tgactatcga acgtaattta
     6241 attggcgcgt actgcacctc actggatatg accggtttct caatcacctt actgaaagtt
     6301 gatgacgaaa cgctggctct ctgggacgcc ccggtccaca ccccggccct taactggggt
     6361 aaataaggag aaagcaatgt cactgagcag aactcaaatt gttaactggc tcactcgttg
     6421 tggcgatatt ttcaacaccg agagcgagta tcttaccggg ctggatcgcg agattggcga
     6481 cgctgaccac gggctaaata tgaaccgagg ctttagcaaa gtggtggaaa aactccctgc
     6541 tatcgcagat aaagatatcg gtttcattct caaaaatacc ggtatgacgc tgctttccag
     6601 cgtcggtggt gccagtggtc cgctgttcgg taccttcttt atccgcgccg cacaggcgac
     6661 ccaggcacgg caaagcctga cactggaaga gctttatcag atgttccgcg atggcgcgga
     6721 cggcgtaatc agtcgcggga aagccgaacc tggcgataaa accatgtgtg atgtgtgggt
     6781 gccggtggtg gaatcgttac gtcagtccag cgagcaaaat ctctctgttc cggtggcgct
     6841 cgaagctgcc agtagcatcg ccgaatccgc tgcacaaagt acgattacga tgcaagcccg
     6901 caaaggccgc gccagttatc tcggtgaacg cagtattggt caccaggatc ccggcgcgac
     6961 ctcggtgatg tttatgatgc aaatgttggc gttagccgca aaagagtaag gaattggtga
     7021 tggtaaacct ggtcatagtt tcacatagca gccgactggg agaaggtgtc ggtgaattag
     7081 cccgtcagat gttaatgagt gatagttgta aaatcgccat tgccgcggga attgacgatc
     7141 cacaaaatcc cattggtacc gatgccgtca aagtgatgga ggccatcgaa tctgttgctg
     7201 atgccgacca tgtgctggtc atgatggata tgggtagcgc attattgagt gctgaaactg
     7261 cgctggaatt gctggctccc gagatcgccg caaaagtacg tttgtgtgct gcgccgttgg
     7321 tcgaaggtac actggcagca acggtcagcg cggcctcggg ggcggatatc gacaaagtta
     7381 tctttgacgc tatgcatgcg ctggaagcca aacgtgaaca actgggttta ccgtcctccg
     7441 acactgaaat ctctgacaca tgtcctccgt acgatgaaga agcccgttct ctggcggtgg
     7501 tcataaaaaa ccgtaacggc ctgcatgtac gtccggcctc ccggctggtt tataccttat
     7561 cgacatttaa tgccgatatg ttgctggaaa aaaacggcaa atgcgtcaca ccagagagta
     7621 ttaaccagat tgcgttacta caagttcgct ataacgatac gctgcgcctg attgcgaaag
     7681 ggccagaagc tgaagaggca ctgatcgctt tccgtcacct ggctgaagat aactttggtg
     7741 aaacggagga agtcgctcca cctactctgc gtcccgttct gcctgtttcg ggtaaagcct
     7801 tttattatca accagtttta tgtacggtac aggcaaaatc aaccctgacc gcggaagaag
     7861 aacaagatcg attacgccag gctattgact tcacgttatt agatctgatg acgttaacag
     7921 cgaaagcaga agccagcggg cttgacgata ttgccgcaat cttttctggt caccatacac
     7981 tgttaggtga tccggaactg ctggcggcgg caagcgaact ccttcagcat gaacattgca
     8041 cggcagaata tgcctggcag caagttctta aagaacttag ccagcaatac cagcaactgg
     8101 atgatgaata tctacaagct cgctatattg atgtggacga tcttctgcat cgcaccctgg
     8161 tccacctgac ccaaacgaaa gaagaactcc cgcagtttaa ctcgccaact attctactgg
     8221 cggagaacat ttatccttcc acagtactgc aactggatcc ggcggttgta aaaggtatct
     8281 gccttagcgc cggaagtccg gtatcccaca gcgccctaat cgcccgtgaa ctggggattg
     8341 gctggatttg ccagcagggt gagaaactgt atgcgataca accagaagaa acgctaacgc
     8401 tggacgttaa aacgcaacgt ttcaaccgtc agggttaagc caccgttcgg atggcatcgt
     8461 tctgatgtca tccggcaatt tac
//
LOCUS       DAZPAB010000165         8332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZPAB010000165 DAZPAB010000000
VERSION     DAZPAB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.165"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(168..566)
                     /locus_tag="ACHUPG_003241"
     CDS             complement(168..566)
                     /locus_tag="ACHUPG_003241"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFR0862745.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(566..793)
                     /gene="vapB"
                     /locus_tag="ACHUPG_003242"
     CDS             complement(566..793)
                     /gene="vapB"
                     /locus_tag="ACHUPG_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFR0862746.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            875..6128
                     /gene="traI"
                     /locus_tag="ACHUPG_003243"
                     /pseudo
     CDS             875..6128
                     /gene="traI"
                     /locus_tag="ACHUPG_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6148..6894
                     /gene="traX"
                     /locus_tag="ACHUPG_003244"
     CDS             6148..6894
                     /gene="traX"
                     /locus_tag="ACHUPG_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFR0862747.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6949..7509
                     /locus_tag="ACHUPG_003245"
     CDS             6949..7509
                     /locus_tag="ACHUPG_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR0862748.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7640..7852
                     /locus_tag="ACHUPG_003246"
     CDS             7640..7852
                     /locus_tag="ACHUPG_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR0862749.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8096..>8332
                     /locus_tag="ACHUPG_003247"
     CDS             8096..>8332
                     /locus_tag="ACHUPG_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR0862750.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1913 a         2086 c         2670 g         1663 t
ORIGIN      
        1 atcagtgaat ccggtgaagt ggtggatatg gccgcttatg aggcatggca gcaggaaaat
       61 catccggaca cctggcagca gatgcagcgt cgtgaagagg tgaacatcaa tgtgcaccgg
      121 gaacgcgggg aggatgttga gccgggagat gatttctgat gaacaggtca gctccagtct
      181 tcagttctca ggccgcccac acgttcaaat tcccgggtgt tattagtcac aataatcagc
      241 ccccgactgc gggcatgacc tgcgatcatt tgatcaaatg gcccgacagg gcgtccctga
      301 cgggcaagtt ctgctcttat ctggccggtg tgtgtggctg cagcagcgtc gtaatccaga
      361 acatcaatgc gggaaacaaa tccctcgatc acagcgagat tacgttcagg catctggctt
      421 ttttctgcac catatatcag ctccatcagg gtgaccgaac tgatacacat tttcccctgg
      481 ttcaggttaa aacgctccct gacgctggcg ggtttgttct ttatcgtaaa aatgcagatg
      541 ttggtatcga gcataaactt cagcatcaga atgactccct ttcttgcatg ccgggctgtt
      601 ccctgttatc cataaaatcg gtgctgacgc tgtgtccgtc gaaccattcg tcccatgtct
      661 ctccggctgg cgtaatgatt ctggttcgtc cgacagcaat cacttcaacg cgttttacgt
      721 tttccggcaa tgcaaccgct tttggcagtc tgaccgcctg gctgcggttg ctgagaaata
      781 cggtggtttc cattatttgc ctccttatgt ggatatgtct tgtgtatatc tattgtggga
      841 ttgataccgg gatatgtcaa agggatatac gtttatgatg agtatcgcgc aggtcagatc
      901 ggccggaagt gccggtaatt attataccga caaggataat tactatgtgc tgggcagcat
      961 gggagaacgc tgggccggcc agggggctga acaactgggg ctgcagggca gtgtcgataa
     1021 ggatgtcttt acccgccttc tggagggcag gctgccggac ggggcggatt taagccgcat
     1081 gcaggatggc agtaacaggc atcgtcccgg ctacgacctg accttctccg ctcccaaaag
     1141 tatctccatg atggccatgc tcggggggga caaacgtctt attgaagcac ataaccaggc
     1201 cgtggatttt gctgtccgtc aggtggaggc cagtgcctcc acctgacgga cggacagtca
     1261 gaaacggtgc tgaccggtaa tctggtgatg gcactgttta accacgacac cagtcgcgat
     1321 caggaaccac agttacacac gcatgcggtg gtgactaatg ttacgcagta taacggcgaa
     1381 tggaagacac tgagcagtga caaagtgggg aaaacggggt tcattgagaa tgtgtacgct
     1441 aatcagattg cctttggcag gctctaccgg gaaaaactga aagagcaggt tgaggcgctg
     1501 ggctatgaaa ctgaagtggt cggtaagcac ggtatgtggg aaatgccggg tgtaccggtg
     1561 gaggcctttt ccggacgctc acagactatc cgggaggccg tcggggaaga cgcctcgctg
     1621 aaatcccggg atgtggcggc gctggatacg cgtaaatcca aacagcacgt cgatcctgaa
     1681 gtcagaatgg ccgagtggat gcagacgctg aaggaaaccg ggttcgacat ccgggcatat
     1741 cgtgacgcag cggaacaacg ggcatatacc cgcacgcaga cacccggacc cgcttcacag
     1801 gacgggccgg atgtgcagca ggcggtgaca caggccattg ccggattaag tgaacgcaaa
     1861 gtgcagttta tgtatacgga cctgctggcc aggacggtcg gcatactgcc gccggaaaat
     1921 ggtgtgattg aacgggcacg cgccggtatc gatgaggcca tcagccgtga gcagcttatc
     1981 cccctcgatc gtgagaaggg gctgttcacg ttcgggattc atatgctcga tgagctgtca
     2041 gtccgggcac tcagtcgtga catcatgaaa cagaaccggg tgaccgtaca tccggagaaa
     2101 agtgtccccc ggacggccgg ttacagcgat gccgtcagtg ttctggcaca ggaccgtccg
     2161 tcgctggcca ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg ggtggctgaa
     2221 ctggtcatga tggcccggga gcaggggcgg gaggtgcaga ttatcgctgc tgaccgtcgc
     2281 tcgcagatga acctgaagca ggatgaacgg ctgtccggtg aactgataac cggacgtcgt
     2341 cagttgctgg aaggaatggc cttcacgccg ggcagtaccg ttatcgtcga ccagggcgaa
     2401 aaactctccc tgaaagagac gttaaccctg ctggatggtg ccgcacgtca taacgtacag
     2461 gtcctgataa ccgacagcgg gcagcgaacc ggtacaggca gtgcactgat ggccatgaag
     2521 gatgccgggg tgaacacata tcgctggcag gggggagaac agcgaccggc caccatcatc
     2581 agtgaaccgg accgtaatgt cggctggccg gagattttgc ggccagcgtg aaagccggag
     2641 aagagagcgt ggcacaggtc agcggagtac gggaacaggc catactgaca caggccattc
     2701 gcagtgagct gaaaacacag ggcgtgctcg gacacccgga ggtaaccatg accgccctgt
     2761 caccggtctg gctggacagc cggagccgtt atctgcggga tatgtaccgc ccgggaatgg
     2821 tgatggagca gtggaacccg gagacacgca gtcatgaccg ctatgtgaca gaacgtgtga
     2881 cggcgcagag tcacagcctg accctgcgga atgcgcaggg cgaaacgcag gtggtgcgta
     2941 tttcttccct ggacagcagc tggtcgctgt tccggccgga aaaaatgccg gtggcagacg
     3001 gtgagcgtct gagggtgaca gggaaaattc ccggactccg cgtctccggc ggtgaccgcc
     3061 tgcaggtggc atccgtcagt gaagatgcga tgacggttgt tgtgccggga cgggctgaac
     3121 cggccaccct gcctgtgagc gattcaccgt tcactgcact gaagctggag aacggctggg
     3181 tggaaacgcc cgggcattcc gtcagcgaca gtgcgacggt ttttgcctcc gtcacacaga
     3241 tggcaatgga caatgccacc ctgaatggtc tggcccgcag tggtcgtgat gtccggctgt
     3301 attcctcact ggatgaaacc cgtactgcgg aaaaacttgc ccgccatccc tcctttacgg
     3361 tggtttctga gcagataaag gcgcgtgccg gtgagacatt gctggaaacc gctatcagtc
     3421 tgcagaaagc cgggcttcac acgccggcac agcaggccat tcatctggcc cttcctgtgc
     3481 tggaaagtaa aaacctggcc ttcagcatgg tggacctgct gacagaggcg aagtcgtttg
     3541 ctgcagaagg aaccggtttt gctgacctgg gaggggaaat caatgcgcag ataaaacggg
     3601 gtgatttact gtatgtggat gtggcaaaag gctatggcac aggcctgctg gtttcccgtg
     3661 cgtcgtatga ggcagaaaag agcattcttc gccatattct cgaaggtaag gaggcggtca
     3721 cgccgctgat ggagagagta cctggcgaac tcatggagaa actgacatca ggacagcgcg
     3781 ccgccacccg tatgatactg gaaacgtctg accgtttcac ggtggtgcag ggctatgccg
     3841 gtgtgggtaa gaccacacag ttccgggcgg tgatgtcagc cgtgaacatg ctgccggaga
     3901 gtgaacgtcc ccgtgtcgtg gggctggtgc ccacacaccg tgcggtcggg gagatgcgca
     3961 gcgccggcgt ggatgcacag acactggcgt cctttctgca tgacacgcag ctgcagcagc
     4021 gcagcggaga aactccggat ttcagcaaca cgctgttcct tctcgatgag agctctatgg
     4081 tgggcaatac cgacatggca cgggcatacg ccctgattgc ggccggtggc ggtcgggctg
     4141 tggccagcgg tgacacggac cagctgcagg ccatcgcgcc cggtcagcct ttccgtctcc
     4201 agcagacgcg cagtgctgcc gatgtggtca tcatgaagga gattgtgcgt cagacgccgg
     4261 aactgcggga ggcggtatac agcctgatta accgggatgt ggaaagggcg ctgtccgggc
     4321 ttgagcgtgt gaaaccgtct caggtgccac gtctggaggg cgcatgggca ccggagcact
     4381 ccgtgacgga gttcagtcac agccaggaag cgaaactggc agaagcgcag cagaaggcga
     4441 tgctgaaagg cgaggctttt ccggatgtcc ccatgacact gtatgaagcc attgtccgcg
     4501 actataccgg caggacaccg gaagcacggg agcagacgct gattgtcacg cacctgaatg
     4561 aggaccggcg cgtactgaac agcatgattc atgatgcacg ggaaaaggcc ggagaactgg
     4621 ggaaggtgca ggtcatggtg cctgtcctga acacagcgaa tatacgcgac ggggagctgc
     4681 gtcgtctctc cacctgggag aataacccgg atgcccttgc cctggtggat aatgtgtatc
     4741 accggattgc cggtatcagt aaggatgacg ggctgataac cctgcaggat gcggaaggta
     4801 acacgcggct gatttcgccc cgggaggcgg tggctgaagg cgtcacgctg tacaccccgg
     4861 acaccatccg ggtggggacc ggtgacagga tacgcttcac gaagagtgac cgggagcgcg
     4921 gttatgtggc caacagcgtc tggacggtga cagcggtttc cggtgacagt gtcacacttt
     4981 ctgacggtca gcagacccgt gtgattcgcc ccggtcagga gcgggcagag caacatattg
     5041 acctggccta tgccatcacc gcccacggtg cacagggggc gagtgaaacc tttgccatcg
     5101 cgcttgaagg cacggaaggt aaccggaaac tgatggccgg ctttgagtca gcctacgtgg
     5161 ccctgtcgcg tatgaagcag catgtgcagg tgtacaccga taaccgtcag ggctggacgg
     5221 atgccatcaa caatgccgta cagaaaggaa ctgcccacga tgtgtttgag ccgaaaccgg
     5281 accgggaggt catgaatgca gagcggctgt tcagtacggc gcgggagctg cgggacgtgg
     5341 cggcaggccg tgccgttctc cgtcaggcgg ggctggccgg gggagacagt cctgcacggt
     5401 ttattgctcc gggacgtaaa tatccgcaac cgtatgtggc actgccggcg tttgaccgta
     5461 acggcaagtc cgccggtatc tggctgaacc cgctgaccac ggatgacgga aacgggctgc
     5521 ggggattcag cggtgaaggt cgggtaaaag gcagcgggga tgcgcagttc gtggccctgc
     5581 agggcagccg taacggagaa agcctgctgg ctgataatat gcaggatggc gtccggattg
     5641 cccgtgataa tcctgacagt ggtgtggtgg taagaatcgc cggtgaaggc cgtccgtgga
     5701 atcccggtgc catcaccggt ggccgcgtgt ggggggatat cccggacaac agcgtccagc
     5761 cgggagccgg aaatggtgaa ccggtcacgg cagaggtact ggcacagcgg caggctgaag
     5821 aggccatccg ccgtgaaacg gaacgtcgcg cagatgaaat tgtccgtaaa atggcagaga
     5881 acaaacctga cctgccggac ggcaaaacag agcaggctgt cagggaaatt gccgggcagg
     5941 agcgtgaccg ggctgccata actgaacggg aagccgcgct gccggagagt gtactgcgtg
     6001 aaccacaacg ggtgcgggaa gcggtccggg aagttgcccg ggaaaatttg ctgcaggagc
     6061 gtctgcagca gatggagcgg gatatggttc gtgacctgca gaaagagaaa accccgggcg
     6121 gagactgata caggaagaca aacactgatg acaacggata acacgaacac gacacgtaac
     6181 gattcactgg ctgcacggac cgatacctgg ttgcagtcat ttctggtctg gtcacccgga
     6241 cagcgggata tcatcaaaac ggtggcactg gtgctgatgg tactggacca catcaatctg
     6301 atattccagc tgaagcagga atggatgttt ctggccgggc gaggagcctt tccgcttttt
     6361 gccctggtgt gggggctgaa tctgtcccgt catgcgcata tccggcaacc ggccattaac
     6421 cggctgtggg gatggggaat tattgcccag ttcgcatatt atctggcagg ctttccttgg
     6481 tatgagggga atatcctgtt tgcctttgca gtggcggcac aggtgctgac gtggtgtgaa
     6541 acgcgcagtg ggtggcgtac agcagcggcc atactcctga tggccctgtg ggggcctttg
     6601 tccggcacca gttacggtat tgccgggttg ctgatgctgg cagtcagcaa ccggctgtac
     6661 cgggcggaag acagagcgga acgtctggcg ctggtcgcct gcctgctggc tgttattccg
     6721 gcgcttaacc ttgccaccag tgatgcagcg gcggtggccg gtcttgtgat gacggtgctg
     6781 accgttggtc tggtgttgtg tgccggaaaa tcattacccc gtttctggcc cggagacttt
     6841 ttcccgacgt tctatgcctg tcatctggct gtgctgggcg ttctggcgct gtgacgggtg
     6901 tggtatcttt ggccgcaaga ggatgattcg tcagaggcag aacacagcat gacagagcag
     6961 aagcgaccgg tactgacact gaagcggaag acagagggaa cagcgcctgt ccgcagccgg
     7021 aaaaccatca tcaatgtcac cacgccacca aagtggaagg tgaaaaagca gaagctggcc
     7081 gagaaagcgg cccgtgaagc agagctggcg gcaaaaaaag cgcaggccag acaggcgctg
     7141 tccatttatc tgaacctgcc cacgctggat gaggctgtga ataccctgaa gccctggtgg
     7201 ccgggattat ttgacggtga cacgccccgg cttctggcct gcggtatccg ggacgtgtta
     7261 ctggaagacg tggcgcagcg gaatatcccg ctctcgcata aaaaactgcg cagggcgctg
     7321 aaggccatca cccgttcaga aagctatctg tgtgccatga aagccggtgc ctgccggtat
     7381 gacacggaag ggtatgtgac ggagcatatt tctcaggagg aggaagcgta tgcgggagcg
     7441 cgtctggcga aaattcgtcg ccagaaccgg ataaaggcag aacttcaggc cgtgcttgat
     7501 gagaaataaa aaaagcctcc cccagagaga ggaggcaggg aaataaggct taaaaggaaa
     7561 taacttccgc ttccgaaagg aagccagtcc actgaattat ttatcacaat aatactgaca
     7621 actaaagggg gacaagaaaa tgaatggatt cagaaacagt tcacggaacg gccaggtctg
     7681 gcgttaccag cgtgccggcg ggcgggccgt tattctggaa gtcagtggac gctggatgga
     7741 agcggcagaa gcatggcgac gggctgcctg tgttgcgcct aggacagact ggcagcagtt
     7801 tgcccggaaa agggctgaac actgccaccg gcgctgccgg ggaagggtgt aacgaaaaaa
     7861 gcagccttca ttactctagg agtcagaagg ctgaaaggag tttatgagat ttgcttctta
     7921 atatccggtc gggcagtatg accggacagt tctgagtagc acagagtctt ttccgcatta
     7981 agtcgatttt ttatattttg tgattagttt cacaaaataa ggtgttattc aatgtgtgct
     8041 gcactattca ggggggctga aatacaggtg gcatttatct gatatggaga ataacatgag
     8101 gaaatatatt ccactggcat tatttatctt ttcatggccg gtattgtctg cagatattca
     8161 tggacgggtt gttcgtgtac tggacggtga taccatcgag gtcatggact cactgaaggc
     8221 ggtgcggatc aggctggtaa atattgatgc accggaaaag aaacaggatt atggccggtg
     8281 gtcaacagac atgatgaaat cactggtcgc aggaaaaacg gttaccgtca ca
//
LOCUS       DAZPAB010000166         8290 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZPAB010000166 DAZPAB010000000
VERSION     DAZPAB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.166"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="ACHUPG_003248"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="ACHUPG_003248"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR0862751.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="ACHUPG_003249"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="ACHUPG_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR0862752.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="ACHUPG_003250"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="ACHUPG_003250"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR0862753.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="ACHUPG_003251"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="ACHUPG_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR0862754.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="ACHUPG_003252"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="ACHUPG_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR0862755.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="ACHUPG_003253"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="ACHUPG_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR0862756.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="ACHUPG_003254"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="ACHUPG_003254"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR0862757.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         2093 a         2114 c         2099 g         1984 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa
//
LOCUS       DAZPAB010000167         8278 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZPAB010000167 DAZPAB010000000
VERSION     DAZPAB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.167"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(412..1608)
                     /gene="hemY"
                     /locus_tag="ACHUPG_003255"
     CDS             complement(412..1608)
                     /gene="hemY"
                     /locus_tag="ACHUPG_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR0862758.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1611..2816)
                     /gene="hemX"
                     /locus_tag="ACHUPG_003256"
     CDS             complement(1611..2816)
                     /gene="hemX"
                     /locus_tag="ACHUPG_003256"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR0862759.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(2838..3578)
                     /gene="hemD"
                     /locus_tag="ACHUPG_003257"
     CDS             complement(2838..3578)
                     /gene="hemD"
                     /locus_tag="ACHUPG_003257"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR0862760.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3575..4516)
                     /gene="hemC"
                     /locus_tag="ACHUPG_003258"
     CDS             complement(3575..4516)
                     /gene="hemC"
                     /locus_tag="ACHUPG_003258"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR0862761.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            4903..7449
                     /gene="cyaA"
                     /locus_tag="ACHUPG_003259"
     CDS             4903..7449
                     /gene="cyaA"
                     /locus_tag="ACHUPG_003259"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR0862762.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7489..7809)
                     /gene="cyaY"
                     /locus_tag="ACHUPG_003260"
     CDS             complement(7489..7809)
                     /gene="cyaY"
                     /locus_tag="ACHUPG_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR0862763.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7857..>8278)
                     /locus_tag="ACHUPG_003261"
     CDS             complement(7857..>8278)
                     /locus_tag="ACHUPG_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFR0862764.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREGNVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1850 a         2179 c         2141 g         2108 t
ORIGIN      
        1 tgaattttat cattcataat aagtatgtgt aggatcaagc tcaggttaaa tattcactca
       61 ggaagttatt actcaggaag caaagaggat tacagaatta tctcataaca agtgttaagg
      121 gatgttattt cccgattctc tgtggcataa taaacgagta gatgctcatt ccatctctta
      181 tgttcgcctt agtgcctcat aaactccgga atgacgcaga gccgtttacg gtgcttatcg
      241 tccactgaca gatgtcgctt atgcctcatc agacaccatg gacacaacgt tgagtgaagc
      301 acccacttgt tgtcatacag acctgtttta acgcctgctc cgtaataaga gcaggcgttt
      361 ttttatgcat cagaaaggtc ccggaggtgc tttcctccgg gtgagaagga actactgtgg
      421 cgggttattc tgcaacgtta acatcaaacc gtcgcgacgc atcgctgcag cttcttccgg
      481 cttgtgcagt ctgtccagcg cgtcggcaag ccatgcgtaa tcgtaggcgt ccggacgttg
      541 tttcagcgcc gcgcggaagg cgagcgatgc ttcctgccat tcaccgtgct tcatcagtga
      601 ctgacccaat gtgctccaca acagcgggcg atcaccgacg tttttgattt gctggcgtag
      661 cactttttcc agctgttccg ggttattggt tttcagacgc gggatcggca gcagtaggcg
      721 atcgtcatac tggcgtttca ggccgtcgat gataatttgc tgggcagtat catgatcgtc
      781 acattcaata agatgttccg ccattgccac ctgcaacgct acctgatgac gcgttttccg
      841 gctttggttt ttccaccagt tacgcaaacc ttcactaccg ttatcggcac gcgcctgatc
      901 catcaggcca atccatgcct gttgttccag cattgcacga tgttcttcat caccaacatg
      961 ggctttcgcc attgatggga tgatatccag cagagaactc catgcccctg tgcggatata
     1021 cgcctgttcc gccagacgta atacttcagg atggcgtggc gtaacttcca gcagcttatc
     1081 cacgccgtgg cgtgcagcat ggttttcatt acgggccagt tgcagacgta cacgggtgat
     1141 ttctaccgga atggtgtcat tgccggccag ctccgctgcg cgttccagat gttggttggc
     1201 gcgtgcttca tcaccacgtt gttgcgcggc ttcggcagcc agtagatagt tcaccaccgg
     1261 ttgttcagcg tgatcggcat ttttcgccat cagcttttca acttgctgat aatcaccttc
     1321 cgccagtttc agcagcgcct gttcggtctg cttacgcgca cggcgacgct tacgtccgac
     1381 aaaccatcca cgggtgtgcg cgccagtgcg gaagatccgc cgcagtagcc actcaatggc
     1441 aaacagcact accatcgcca gaatcaatat gatcgccagg cccgtgacgc tggtttcgat
     1501 attgtagttg tcggtctgga tcagcacata accctgatga ccggcaatca tcgggccaac
     1561 cactatcccc gcaatcagca aaacaaagag taataacact tttagcatgg ttattctcct
     1621 tgcggcgcgg ctgccggagt atcaccttgc ggtgcaggtg caggtgcagg tgcaggtgca
     1681 ggtgcaggtt tagcttccgt tgtccccgct gccggttgtg ccagcaggtt acgcacgcga
     1741 gtctgcatca gtttttccag catcgcctgg ctttgcaggg tttccggaag atccatcgag
     1801 atattttgct ggcttaactg gtccacctcg tcgaggaacg ctttggtggt ggcatcatca
     1861 gtatcgtagt aagcacgtac ccaggtggag acgttctcca gcgcctggcg ataagtctct
     1921 tcctgatgac gcggtacagc ttgtgctgcg accagcaggc gagagcgaat attttcgcgc
     1981 agatagatat cctgatttgg cgctaacagc ggtacggcgg tgtcatcacg acggcgaatc
     2041 gtaatgaagt tgtccataaa gttctgccag cttttttgca gattgatacg ccattcgctg
     2101 atggaactgg aaagctcttc accgtctgaa tccatcggcg aaccatcgct gtcattatcg
     2161 gccagacgca ggttatctac ctgatttgaa agctgattaa gcttaaggat gatgccgtca
     2221 taatccacct gcgatactgc agaaaggctg gcgatatcat cggtaattgc ccgacgaacg
     2281 gtaatcagac tcgggtcatt catatccgcc aggctggcgt ctgcactttt cagcaacgct
     2341 gcagaggtcg tgacgtcctg atcgctccac agcttccgtc cggcgagttt caccagaaaa
     2401 tcggcctgag ccagcagcca ggttttagca tcgctgccgg aaatggtggc gatcttttgt
     2461 tggacttcat ccaactgttt tgccagcgtt tcttgctgac gattcgcctg cttaagctgc
     2521 gcagcttgtt gcttaataat gccttccagc tcggcttttt ggctctcctg ggctttttgc
     2581 aatgccgtca gttggttagc cagggcatcg ctggtggcgg tctgattgac ggcctgttgt
     2641 ttaccccagc catacaaacc gacacccgcc gccagagcaa tagcgatagc caccgcgctg
     2701 agaatcaatg cagtattgtt cttactcttt ttttctgttg cgacaggttg tgacgtggtg
     2761 tccacggcct ccctggtctc ttcaaccacg gcggaggttt tttcttgttc cgtcattatg
     2821 gcttcctgtt atgagagtta ttgtaatgcc cgtaaaagcg catcgttgtc agcgttatcg
     2881 gcgaccttaa tgtcttgcca gcccagttcc cgggcgagtt tcgccaaacg ctcactgacg
     2941 accaatagtc gacagtgtag taaccagtgc tcacgatacc attgtgggat cagcgaccag
     3001 agttgctgca acatttcacc gctggtaaca acgaccatcg tcacctcgcg ggattgccag
     3061 cgcatcgctt cttctgcacc atcgtaatgg attgcgcatc gttgataaca ttcacaaaaa
     3121 gtgacctcag caccgcgcac cgtcagggta tccccaatta gctcacgacc gccattgcca
     3181 cgtaatatca gcgcacgttt gcccgcaata ttttgtaatt caggtaattg tagcaagact
     3241 tcgctgattt cccgatcctg tgggtagaga atcttctgtc cgcttacggt atgtagcgcc
     3301 agtgcggtgg tgcgtccaat ggcgaaataa gtaggtagtt ggggccattt acgatcttgc
     3361 tgatgcagct gtgattgggc aaaagcaacc gcgtgttgcg agagggcaaa caacagatcg
     3421 ctctccccca gcgctgccag ttgatcagca agttgcggta attgtcgacc cggagaaaac
     3481 tcaatcagcg gaaaatgcca ggccacctgc cccagtgtgc gcagacggct cactaactct
     3541 tctccagcgg gagacgggcg ggtgaccagg atactcatgc cggggcctct ccgttataga
     3601 cttcagcgag gatctcgcgc gcgccgttat tcagtagctc ttctgccagc gaaatcccca
     3661 tttgttcggc atcttgcggc gcaccgcggc gttcaccgcg aataatctgc gaaccgtccg
     3721 gcgcgccgac cagcgcacat agccagattt cgccatcaat aagctcggcg tagctaccaa
     3781 ttggcacctg acatccgcct tcgagacggg tattcatggc gcgttctgcg gtaacgcgca
     3841 gtgcagtttc gtggtgattc agcgcggcaa gcagctcgcg agtgcgtgta tcatcaaggc
     3901 ggcattcaat accgactgca ccttgtccta ccgccggaag agaaatctcg ggtggtaacg
     3961 cggcgcgaat gcgggactcc agacctaaac gttttagtcc ggctacggca agaatgatgg
     4021 catcgtattc gccgttatct agtttgctca ggcgagtgcc gacgttgccg cgcagggagc
     4081 ggataatcag atccgggcgg cgttcagcca gttggcactg gcgacgtaaa ctggacgtcc
     4141 cgacgatact gcctgctggt aacgcatcca gactgtcata gttattggac acaaaggcat
     4201 cgcgaggatc ttcacgctcg caaatagtga ccagccccag accttgcggg aattcaaccg
     4261 gcacatcttt cattgaatgt acggcgatat cagcgcgatt ctccaggagc gcgacttcca
     4321 gctcttttac aaataagcct tttccgccta ctttcgccag cggcgtatca agaatcacat
     4381 cgccgcgcgt caccatcggt accagttcaa cgaccaggcc cggatggctc gccatcaact
     4441 tgtctttgac atagtgtgcc tgccagagtg caagtgggct ttggcgtgtg gcaattctta
     4501 aaacattgtc taacatgctt gttaccgtca ttatcatccg tggtccatcc taacatcctt
     4561 gccagagtga tgtcagtgtt gtggtgaaac gtagacgcct gcgcaaaccg taaaatgagg
     4621 tctggcagtg gatcctgaca ggcgtttcac gccgtcgtaa taaggaattt acagagaata
     4681 aacggtgcta cacttgtatg tagcgcatct ttctttacgg tcaatcagca aggtgttaaa
     4741 ttgatcacgt tttagaccat tttttcgtcg tgaaactaaa aaaaccaggc gcgaaaagtg
     4801 gtaacggtta cctttgacat acgaaatatc ccgaatgccg cgtgttaccg ttgatgttgg
     4861 cggaatcaca gtcatgacgg gtagcaaatc aggcgatacg tcttgtacct ctatattgag
     4921 actctgaaac agagactgga tgccataaat caattgcgtg tggatcgcgc gcttgctgct
     4981 atggggcctg cattccaaca ggtctacagt ctactgccga cattgttgca ctatcaccat
     5041 ccgcgaatgc cgggttacct tgatggtaac gttcccaaag gcatttgcct ttacacgcct
     5101 gatgaaactc aacgccacta cctgaacgag cttgaactgt atcgtggaat gtcagtacag
     5161 gatccaccga aaggtgagct tccaattact ggtgtataca ccatgggcag cacctcgtcc
     5221 gtagggcaaa gttgttcctc tgacctggat atctgggtct gtcatcaatc ctggctcgat
     5281 agcgaagagc gccagttact acaacgtaaa tgcagcctgc tggaaagctg ggccgcctcg
     5341 ctgggtgtgg aagtcagctt cttcctgatt gatgaaaacc gcttccgtca taatgaaagc
     5401 ggcagcctgg ggggcgaaga ttgtggctcc acccagcata tactgctgct tgacgaattt
     5461 tatcgtactg ccgtgcgtct cgccggtaag cgtattctgt ggaatatggt gccgtgcgac
     5521 gaagaagagc attacgacga ctatgtgatg acgctttacg cgcagggcgt gctgacgcca
     5581 aatgaatggc tggatctcgg tggcttaagc tcgctttctg ctgaagagta ctttggtgcc
     5641 agcctttggc agctctacaa gagtatcgat tccccataca aagcggtgct gaaaacactg
     5701 ctgctggaag cctattcctg ggaatacccg aacccacgtc tgctggcgaa agatatcaaa
     5761 cagcgtttgc acgacggcga gattgtatcg tttggcctcg atccatactg catgatgctg
     5821 gagcgtgtta ctgaatacct gacggcgatt gaagatttca cccgtctgga tttagtacgt
     5881 cgctgcttct atttaaaagt gtgcgaaaaa ctcagccgtg aacgcgcctg tgtaggctgg
     5941 cgtcgcgcag tgttgagcca gttagtgagc gagtggggtt gggacgaagc tcgtctggca
     6001 atgctcgata accgtgctaa ctggaagatt gatcaggtgc gtgaggcgca caacgagctg
     6061 ctcgacgcga tgatgcagag ctaccgtaat ctgatccgct ttgcgcgtcg caataacctt
     6121 agcgtctccg ccagtccgca ggatatcggc gtactgacgc gtaaactgta tgccgcgttt
     6181 gaagcattgc caggtaaagt gacgctggta aacccgcaga tttcacccga tctctcggaa
     6241 ccgaatctga cctttattta tgtgccgccg ggccgggcta accgttcagg ttggtatctg
     6301 tataaccgcg cgccaaatat tgagtcgatc atcagccatc agccgctgga atataaccgt
     6361 tacctgaata aactggtggc gtgggcatgg tttaacggcc tgctgacctc gcgcacccgt
     6421 ttgtatatta aaggtaacgg cattgtcgat ttgcctaagt tgcaggagat ggtcgccgac
     6481 gtgtcgcacc atttcccgct gcgcttacct gcaccgacac cgaaggcgct ctacagcccg
     6541 tgtgagattc gccatctggc gattatcgtt aacctggaat atgacccgac agctgcgttc
     6601 cgcaatcagg tggtgcattt tgatttccgt aagctggatg tcttcagctt tggcgagaat
     6661 caaaattgcc tggtaggtag cgttgacctg ctgtaccgca actcgtggaa cgaagtgcgt
     6721 acgctgcact tcaacggcga gcaatcgatg atcgaagccc tgaaaactat tctcggcaaa
     6781 atgcatcagg acgccgcacc gccagatagc gtggaagtct tctgttatag ccagcatctg
     6841 cgcggcttaa tccgtactcg cgtgcagcaa ctggtttctg agtgtattga actgcgcctt
     6901 tccagcaccc gccaggaaac cgggcgtttc aaggcgctgc gcgtttctgg tcaaacctgg
     6961 ggcttgttct tcgaacgcct gaatgtatcg gtacagaagc tggagaacgc catcgagttt
     7021 tatggcgcta tttcgcataa caaactgcac ggcttgtcag tgcaggttga aaccaatcac
     7081 gtcaaattac cggcggtggt ggacggcttt gccagcgaag ggatcatcca gttctttttc
     7141 gaagaaacgc aagacgagaa tggctttaat atctacattc tcgacgaaag caaccgggtt
     7201 gaggtgtatc accactgcga aggcagcaaa gaggagctgg tacgtgacgt cagtcgcttc
     7261 tactcgtcat cgcacgaccg cttcacctac ggctcaagct tcatcaactt caacctgccg
     7321 cagttctatc agattgtgaa ggttgatggt cgcgaacagg tgattccgtt ccgaacaaaa
     7381 tcgattggca acctgccgcc cgccaatcag gatcacgata cgccgctatt acagcagtat
     7441 ttttcgtgat gagcgtgccg gaaagcgagg cttatccggc atgcaatctt agcggaaact
     7501 gactgtttca cccgcctgtt gcgtcgccgc ctgttccagc aaatcccaga aggtttcgcc
     7561 gctgcgatcg caaatccatt catcgccttt caggtcaaaa tggtagccgc cctgtttggt
     7621 tgccagccac acctggtgca gcggctcctg gcggttaata atgattttgc tgccgttttc
     7681 aaaggtaatg gtcagtacgc cgccgttgat ttcgcagtcg atatcgctgt cgccatccct
     7741 gtcgtccaga cgctcttcaa tggtcagcca cagttgatca gccaggcgat gaaattcact
     7801 gtcgttcatt gttgtatcct gtttttaagt gatggcggca gtatagcggc atggggtcag
     7861 ggcttcaaag tttgcacctc tgctgctgcg ttccggcatg attcatccgt caccggaata
     7921 atgatgtctc tgttttgaac ttcacgtacg ttcccctctc tgacacgagt tttcgccggg
     7981 cagggggagg gcgtaatcct tacctctgta tcggtccatg tataaacatt gcgatcgtca
     8041 taagcgaagc gatcgttttt agttagcgaa ccgttaactg gaataatttg caagctcttt
     8101 gaccgtaatt ggcggataaa gtaagcatgc tgtttgccgc tattaaatga gcgatcaatg
     8161 accaggtctg gcgtatccag cgggattatc tcaagctggt cttcaccaaa tgtcgataat
     8221 tttgttacat acagccgatc tttatattgc gcaatattgc cagtaatcag atggaatt
//
LOCUS       DAZPAB010000168         8159 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZPAB010000168 DAZPAB010000000
VERSION     DAZPAB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.168"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(355..1548)
                     /gene="tyrB"
                     /locus_tag="ACHUPG_003262"
     CDS             complement(355..1548)
                     /gene="tyrB"
                     /locus_tag="ACHUPG_003262"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR0862765.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1801..2880)
                     /gene="alr"
                     /locus_tag="ACHUPG_003263"
     CDS             complement(1801..2880)
                     /gene="alr"
                     /locus_tag="ACHUPG_003263"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR0862766.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(2933..4348)
                     /gene="dnaB"
                     /locus_tag="ACHUPG_003264"
     CDS             complement(2933..4348)
                     /gene="dnaB"
                     /locus_tag="ACHUPG_003264"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR0862767.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4431..5414
                     /locus_tag="ACHUPG_003265"
     CDS             4431..5414
                     /locus_tag="ACHUPG_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR0862768.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5580..5822)
                     /gene="pspG"
                     /locus_tag="ACHUPG_003266"
     CDS             complement(5580..5822)
                     /gene="pspG"
                     /locus_tag="ACHUPG_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR0862769.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(5956..6993)
                     /gene="dusA"
                     /locus_tag="ACHUPG_003267"
     CDS             complement(5956..6993)
                     /gene="dusA"
                     /locus_tag="ACHUPG_003267"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR0862770.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(7356..8063)
                     /locus_tag="ACHUPG_003268"
     CDS             complement(7356..8063)
                     /locus_tag="ACHUPG_003268"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR0862771.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            complement(8087..>8159)
                     /gene="yjbL"
                     /locus_tag="ACHUPG_003269"
     CDS             complement(8087..>8159)
                     /gene="yjbL"
                     /locus_tag="ACHUPG_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR0862772.1"
                     /translation="AKIDLFIQRITHNLWHARKKMCF"
BASE COUNT         2151 a         2093 c         1901 g         2014 t
ORIGIN      
        1 aagagttctc tttaacatta gaatttaacg atactataat tgaatctcag aaagtcacag
       61 gcaataaggc aaagcatatt cttgaggata tagaaatatt ctatcgtaac aaacccgata
      121 cttattatta aatttaataa tggttagatg tgcagaaatt attactccac acgaaaaaat
      181 agggaaagga attccagata attgggataa acttaacaat aaaagaactg acaacgtttt
      241 attatctctc acgtagaaca acggcatcaa aaaaaatcat ctacaaagaa atagtgaaag
      301 cgacgacagt ttaatttcac tgcaggctgg gtaactccag cctgctttcc tgcattacat
      361 caccgcagca aacgcctttg ccacacgttg tacatttgcc gtatttaacc cggcgacaca
      421 catgcgaccg ctggcgatga gatagacacc aaattcttca cgtagtcggt caacctgagc
      481 ggcacttaaa ccggtataac tgaacatgcc gcgctgatta agcagataat cgaaattgcg
      541 ttctggcatc tctgtgctta ataccttcac caattcctga cgcattgcca gaatgcgagt
      601 acgcatctct tctacttccg ccagccagct ggctttcaat gcctcgtcat tcagcactgc
      661 agccaccacc tgcgcaccaa aattcggcgg gctggagtag ttgcggcgaa ctgttgcttt
      721 caattgcccc agtacgcggc cagcggcttc ggcatcttca cacagaacag aaagtccgcc
      781 gacgcgctcg ccgtaaaggg agaaaatttt cgagaacgaa ttgctcacca gagcgggtaa
      841 tccagcactg gcaatggcgc gaatagcgta ggcatcctct tccataccgg cactaaatcc
      901 ttgataggca atatcgagga atgggataag ctcgcgggct ttgagaattt caatcaccgc
      961 atcccactga tcattagtga gatcggcacc cgttgggttg tggcaacatg gatgcagcaa
     1021 cacaatactg cgggcaggta atgttttcag cgtcgccaac aggtcattaa agcgcacgct
     1081 gttagtcact tcgtcatacc aggggtaagt actcacttcg aatccagccc cggcgaatat
     1141 tgctacgtgg ttttcccaag taggatcgct gacccagacg cctgattccg ggaagtagcg
     1201 tttcaggaaa tccgcgccca ctttcaatgc ccctgagccg ccaagggttt gaatggttgc
     1261 tacgcgctgt tgttgcagta ccggatggtc cgcaccaaac agcagcggcg caatggcatg
     1321 gcgatagctg ttaagccctt ccatcggtaa ataaagcgaa gcgccatggg gctgcgcatt
     1381 caggcgcgct tccgcctccg ccacggcttt caattgtgga ataattccgt cttcgttgta
     1441 gtacagaccg atacttaaat tcactttgtc gctgcgaggg tcttctttaa aacgctccat
     1501 aagcgtaaga atcgggtcgc cagcgtaggc gtcaactttt tgaaacacgc gatggttctc
     1561 caggtttacg ggcaggtggt taaaacacaa taaaccggaa gaaggcgaag atcgagtgga
     1621 tgttcaggag cgaacggcaa ttagcaacag agtgagacta atgataaacg tacatccgcc
     1681 agaggcacga cctttataag agcatgaaag aacatcaact tattgaattt ttaggatttt
     1741 attggccgga taaggcattc acgccgcatc cggcacagac aatcaaatat tacagaacga
     1801 ttaatccacg tatttcatcg cgacccttga agtcaggcgc gtaataagtt cgtaagcgct
     1861 tactttcgtc atttcagcga tacgttctac gggcaaacct tcgccccata aaatgaccgg
     1921 atccccggct ttgtcctgcg cctgtggacc taagtctacg cagatcatat ccatcgccac
     1981 gcgcccgaca atcggtactt cgcgaccgtt caccagcact ggcgtaccgg acggcgcggc
     2041 gcgcggataa ccatcgccat agcccatcgc gactacgcca agacgggtat cacgttcgct
     2101 tacccaggtt ccaccataac caacaggctc tccggcttta tgctcacgca cggcaatcag
     2161 gctggaggtt agtgacatca ctggctgaca gccaaaatcg gcaccggtgg agcgatcttc
     2221 cagcggcgag acgccataaa gaatgatgcc cgggcgcacc cagtcaaaat gcgactgtgg
     2281 ccacagcaga atgccacccg acgcggcaat ggaacgttga ccaggtttgc cttcgcaaaa
     2341 ggtattaaag atagcgagtt gtttctcggt tgcgccacat tttggttcat ccgcgcgcgc
     2401 aaaatggctg acgatattca ccggctgacg aacgtttttg cactgggtca ggcgatgata
     2461 aaacgcctca gcctgttccg gccttacgcc cagacggtgc ataccggtat cgagtttcat
     2521 ccagacggta accggctcgt ccaagctagc ctcttccagc gcagccagct gttcttcgtt
     2581 atgcacggcg gtatgaaaat gttgcgcaga aatcgtcggc agatctctgg catcaaaaaa
     2641 gccttcgagt aacagtacag gtttggtgat tccccccgca cgcagtcgca gagcttcttc
     2701 gagacgggct acgccaaagg cgtcagcatc ggggagcgtt cgcgcggtct caagaagacc
     2761 gtgaccataa gcgttcgctt tcaccaccgc aaccatttta ctggcaggtg ccagttcacg
     2821 aagacgttgc aggttgtgtc gcagagcgcg gcggttaatc acaacagttg ccgcttgcat
     2881 ttgtgttcct tgataattgt ttgctttaat tacctaattc ataaaataat tattattcgt
     2941 cgtcgtactg cggccccgca tagttgtcga agcgcgacca ttgaccgtta aaggtcaggc
     3001 gtaccgtccc gattgggccg ttacgttgtt taccgataat aatttccgcg atgcctttta
     3061 aatcactgtt ttcgtgatac acctcatcac gatagataaa catgatcaag tccgcatcct
     3121 gctcgataga gccagattca cgcaggtcgg agttgaccgg acgtttgtcg gcacgttgtt
     3181 ccagagaacg gttcaactgg gacagcgcca ccaccggcac gttcagttct ttcgccagtg
     3241 ctttcagcga gcgggagatt tctgcaattt ccagcgtacg gttatcggaa agcgccggta
     3301 cgcgcatcag ttgcaggtag tcgatcatga taagcccgat gccgccgtgt tcacgggcaa
     3361 tacggcgtgc gcgggaacgc acttccgttg gcgtcaggcc ggaagagtca tcgatataga
     3421 tattgcgttt ttcgagcaaa atacccatgg tgccggaaat gcgcgcccag tcttcatcat
     3481 cgagctgccc ggtacggatt ttagtctggt caacgcgcga cagcgacgcc agagaacgca
     3541 tcatgatctg ttctgatggc atctccagcg agaagataag tactggttta tcctgcaaca
     3601 ttgccgcgtt ttcgacgagg ttcatcgcaa atgttgtttt acccatcgac ggacgcgcgg
     3661 cgacgatgat caaatccgac ggctgcaagc cagcggtttt tttgttgaga tcgtcataac
     3721 cggtgtttac cccggtaacg ccatcgtgtg gctgctgaaa caactgctca atacgcgcca
     3781 cggttgcgtc gagcacatcg gcgatgttct tcggcccttc gtctttgttt gcacgacttt
     3841 cggcaatttt aaagacgcgg gattcagcaa ggtccagcag atcttcgctg gtacgcccct
     3901 gcggatcaaa accggcttca gcaatctcat tcgcaaccga gatcatctca cggacaacgg
     3961 cacgttcacg cacgatgtcc gcataagcac tgatgttcgc cgcacttggc gtattttttg
     4021 acagctctgc cagataagca aaaccaccga cgctatcgag ttgcccctgg cgttccaacg
     4081 attccgcaag ggtaatcaga tcgataggac taccgctttc ctgcaaacgc gccatttcag
     4141 taaagatatg acggtgtggg cgggtgtaaa aatcgtctgc taccacacgc tcggctacat
     4201 catcccagcg ttcgttatcc agcattaaac cgcccaacac cgactgctcc gcttcgatcg
     4261 agtgcggagg cactttcagc ccggcaactt gtggatcgcg ttcgcggggt tcagcctgct
     4321 gtttgttgaa gggtttattt cctgccatag tgaatggagt taccgaaata aagaatgggt
     4381 cgaaacttta ccatatgaag cagaccctga cgatacgttc tggaggacac atggcaacac
     4441 gaattgaatt tcacaagcac ggtggcccgg aagtacttca agccgtagag ttcactcctg
     4501 ccgatccggc ggagaatgaa atccaggtcg aaaataaagc catcggcatc aattttatcg
     4561 acacgtatat ccgcagcggc ctttacccgc cgccatcgct acccagcgga ttaggtaccg
     4621 aagcggcagg catcgtgagt aaagtcggca gtggtgtaaa gcatattaag gctggcgatc
     4681 atgtagtcta tgcgcagtcg gcgttaggcg cttacagctc tgtgcataac attaatgcgg
     4741 ataaagcggc gattctgcct gcggcaattt cttttgagca agctgcggca tccttcctga
     4801 aaggcttaac ggtttattat ctgctgcgca aaacctatga aattaaaccc gatgaacagt
     4861 tcctgttcca cgcagcggct ggcggcattg gcttaattgc ctgccagtgg gcaaaagccc
     4921 tgggcgcgaa actcatcggc accgtaggaa ccgcgcaaaa agcgcagagc gcgctaaaag
     4981 ccggcgcgtg gcaggttatt aactatcgtg aagagaatct ggtcgagcga ttgaaagaga
     5041 tcaccggcgg taagaaagtg cgcgtagtgt acgattccgt gggcagagac acctgggaac
     5101 ggtcgctgga ttgcctgcaa cgccgcggct taatggtcag ttttggcaac tcatcaggtg
     5161 cggttaccgg tgtgaactta ggcattctca atcaaaaagg ctcgttgtat gtgacacgcc
     5221 cttccctgca aggctatatc accacgcggg aggaattaac cgaggccagt aatgaactgt
     5281 tctctttgat tgccagcggt gtgattaagg tcgatgtcgc cgagcagcag aaatatccgc
     5341 tgaaggatgc gcagcgtgcg catgagattc tggaaagccg ggcgacgcaa ggttccagcc
     5401 tgctgattcc ataaaagaaa tagggcttcc acctgggaag ccctttcttt ttatagttcg
     5461 gctgtatgta gggtacagca cgatgaatct gttagaggcg caatagtgac agatttgatt
     5521 atcaattcct attttgttct aaggataaaa ccttaggttg tgatcatccg cacaatccct
     5581 tagtaacgcc agcggtcata acgctgatat ttcggcattt ttggtgcttt aatcgcctta
     5641 ataacccaca ccaccgcaat cgccagtagt aaccacggca gcaacttaat catcaatgcc
     5701 agcataccgc cgaggaacat aatggccgtc gccacaacca gcgcggcgat aatgcccagc
     5761 aacgaaacgc cggtgaccat cagcatgaca aaaaagccaa tcacaaaaag tagttccagc
     5821 atgatgctct cccaaatatg aaatctcttg ctggcattac aagaatcatg ccaaaaataa
     5881 tctattgatt taacagcaaa acgccccgcg acggtgcgca gggcgtggtg aatttgacta
     5941 ctttttggtg aaaagttaac gcttatccgc caccagtttg agcgcgtgtt ccagcacgtt
     6001 aatgtcagca cccgctttat gggcattttc acttaaataa cgccgccact gccgcgcgcc
     6061 aggaataccc tggaacaaac ccagcatatg ccggataata tggccgagat acgtcccctg
     6121 gctgagttca tgctcaatgt acggatacat ggcacgcact actgccaccg gatcggcatc
     6181 ggtatccgag gaaccaaaga tctcccggtc taccgccgcc agaatacccg gattctgata
     6241 cgcctcgcgc ccgaccatca cgccatccat atgttgcaaa tgcgctttag cttcttccag
     6301 cgacttgata ccaccgttaa tcgacattgt cagatgcggg aagtcacgct tcagttgata
     6361 cacacgcgga taatcgagcg gcgggatttc acggttttct ttcgggctta accccgaaag
     6421 ccaggcttta cgtgcgtgga tgatgaacat ctcacactcg cctttgccgg aaacggtatt
     6481 gatgaaatcg cagagaaatt catagctgtc ctggtcgtcg atgccaatac gcgttttcac
     6541 cgtcaccgga atcgacacca catcgcgcat cgctttcacg cagtcggcaa ccagctgcgc
     6601 attacccatc agacacgcac caaacatgcc gttctgcacc cggtcagacg ggcagccgac
     6661 attcaggttg atctcatcat atccgcgcgc ttctgccagc tttgcacact gcgccagcgc
     6721 cgccggatcg ctaccgccca gttgcaacgc taccggatgt tcttcttcac tgtacgccag
     6781 ataatcacct ttaccgtgaa ttatcgcccc tgtggtcacc atttcggtat aaagcaacgt
     6841 attgcgggaa agcagacgca agaaatagcg gcaatgtctg tccgtccagt cgagcattgg
     6901 tgcaacacta aaccgaccac tccagtgaac gtcagttttt tcaggcattg cgctggtttg
     6961 gttgattttt tgcatttcag aattaccgtg cattttcaaa tgtagagatt attttatcga
     7021 tatatcatgg ggttatgtta ttcagcttca ctgttcagga ggctcaatag cggggtacta
     7081 taccataaca acaggaagcg cctgtctcat tgcaaaagaa aattgagatc ctctcaaggc
     7141 atgaagctct cacgaagtga tggaaataat cttattagcc gttaactttg ttaaggccaa
     7201 tgataaacaa tccaggttcg acgataaata aaaaatcaca cattaaactc tggtgatata
     7261 tctccctgct aatagcattg atagagaaaa aagaacccaa taaatattgg gtccttttat
     7321 ataatgcctt ccatactatc gaagaacttc acatattatt tctccgattt aaccccgaac
     7381 aagtcataaa ttaatttaga agtgtctgta agtattttaa tctcttcctt tgaggttggg
     7441 tcaaacgact tcgcgaagct aattaattgt ggtgcagcat ctcgcatttt gcttaaaata
     7501 tcaggatcga gcgttccttc attcaccaga tgtgacatct tatccagata gccatcaaaa
     7561 ttcatttctc ccccatctgc cagacgcttc atctctccca ggtacttctt catatcacgt
     7621 tgatataatt tttcaaactg agctttcagg tagctctcat tattttcatt aatatacact
     7681 gtttctgata aatctgcgta ggcactagaa tatgaaagtg aaattaacag cgaagcaaat
     7741 aattttaagc tgttgtcatc atgttcctgg caggcattaa caaacgttaa aaaaccagaa
     7801 cctattgctt gaaaatgtat attcgctaaa ggttcattat ccttagattc ctcataaaat
     7861 agctgaaccg tggagggaag gccattctta gtttctacat caaaagtaca ttgttcaatg
     7921 ggcattgcat ctttattatc cccaggatta catatactat taatcgcgaa atgaaaaata
     7981 gcccggtcaa caactgatgc aataaagtca cgatcgttta aatcctcatc agtgcaatcg
     8041 tcagtatact tattgaccca cattttgaga tcatttatgt gctcaattaa aaacacattt
     8101 ttttacgtgc atgccataaa ttatgcgtga ttcgttgaat aaacaaatct atttttgca
//
LOCUS       DAZPAB010000169         8153 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZPAB010000169 DAZPAB010000000
VERSION     DAZPAB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.169"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..972
                     /locus_tag="ACHUPG_003270"
     CDS             <1..972
                     /locus_tag="ACHUPG_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0862773.1"
                     /translation="TRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSH
                     LSLKGIVIKDEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNF
                     AQIKMADGTLNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYV
                     NLFKADLSFSKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICT
                     GSNMANVNFNNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENI
                     PIDSDTLVTLQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(1126..2775)
                     /locus_tag="ACHUPG_003271"
     CDS             complement(1126..2775)
                     /locus_tag="ACHUPG_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR0862774.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2926..4275)
                     /gene="ghxP"
                     /locus_tag="ACHUPG_003272"
                     /gene_synonym="yjcD"
     CDS             complement(2926..4275)
                     /gene="ghxP"
                     /locus_tag="ACHUPG_003272"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR0862775.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4981..5445)
                     /gene="soxR"
                     /locus_tag="ACHUPG_003273"
     CDS             complement(4981..5445)
                     /gene="soxR"
                     /locus_tag="ACHUPG_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR0862776.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5531..5854
                     /gene="soxS"
                     /locus_tag="ACHUPG_003274"
     CDS             5531..5854
                     /gene="soxS"
                     /locus_tag="ACHUPG_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR0862777.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5857..7434)
                     /gene="pdeC"
                     /locus_tag="ACHUPG_003275"
                     /pseudo
     CDS             complement(5857..7434)
                     /gene="pdeC"
                     /locus_tag="ACHUPG_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            7863..8144
                     /locus_tag="ACHUPG_003276"
     CDS             7863..8144
                     /locus_tag="ACHUPG_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFR0862778.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         2267 a         2088 c         1863 g         1935 t
ORIGIN      
        1 acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt atattttata
       61 aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa tggtggttac
      121 gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt atccaattcg
      181 aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat gtataaaacg
      241 aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct cgatgactca
      301 aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc tatgcatgtt
      361 caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct tgactattca
      421 aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc gccatatgtt
      481 aatttattta aagccgacct tagtttctct aaactcgatt taattaactt tgaacatgct
      541 gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt aattgatagc
      601 aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg taccggctct
      661 aatatggcta atgttaattt taataatgcc aatttaagca actgccattt caactgttct
      721 attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt cgatgaggct
      781 agcgtccagg gaatgggcat ttccattctc cgtggggagg aaaatatccc cattgatagt
      841 gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca tactggcatg
      901 tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga tattatgcaa
      961 cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa gagagataac
     1021 ttatttgttt tagaatcatg aatgttcgcc cgagctaacc tgttaaaggt gcaaaagatt
     1081 gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact gattttcctc
     1141 aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg tttcgttgct
     1201 gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca atgccgccag
     1261 acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt tacgtccatc
     1321 ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca actggttatc
     1381 gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg ccacaatcgc
     1441 cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg aatggtccgc
     1501 gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca gcgagaagag
     1561 aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt taccatccgg
     1621 caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca ccccagcgaa
     1681 agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct tcttcttcag
     1741 gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca gcatcagcgc
     1801 cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag ggtcgatttc
     1861 tgccgccatc agcgacgtct ccagaatacc cggcagttgc agacctaaca gcaggaacac
     1921 catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca ggcgcattgc
     1981 cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg caaccgcagc
     2041 gaggatgccc gaaacgccaa tatgttcggc aatcagataa gaagcgaatg gcagcaagaa
     2101 cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga atcgcagcga
     2161 gcgaccatac agccagctca ccacaaaacc ggcaagaata ccgccaatgg ctactttcat
     2221 aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc cccatcgcca ctgccacggc
     2281 aaacttcaac gacaccaggc cggaagcgtc attcatcaac gcttcgccct gcaaaatgcc
     2341 catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca cagcatcggt
     2401 cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg gcaccaccca
     2461 gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg cgaggccgaa
     2521 aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat cagcgaacag
     2581 caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat gcaaaccaaa
     2641 cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg ggatctgaaa
     2701 gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta tcagtatggt
     2761 gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag aaagacgtta
     2821 tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc ggagaaaata
     2881 aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga tagcccagcc
     2941 acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt tcaacttgcg
     3001 ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca tgatgcctgt
     3061 tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca tcgcatcaac
     3121 aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa cgtacatcag
     3181 cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga acagaatcag
     3241 caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg ccgccgtacc
     3301 cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag agaaaacgct
     3361 gctcatggag tcagtggtca gtgctttccc gccgtcgatg atctgcccat ctttatccag
     3421 caggttcgcc tggccggcga cagcgcggat agttccggtg gcatcaaata ctgccgtcat
     3481 caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa tgtccaggct
     3541 gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa cgccggagaa
     3601 atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg tcagcagaat
     3661 gccaccaggg actttcagtt tttccaagcc gatgatcacc gccagacctg ccagtgacat
     3721 aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa gcgggttttt
     3781 aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga taccgatccc
     3841 cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga tacccgtggc
     3901 agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga caggtacgct
     3961 aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagggaga tggcgcaacc
     4021 aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc cggcaaccag
     4081 acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca tacctggaac
     4141 gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca cttcctgacg
     4201 gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc cggtacgcgc
     4261 tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa gatgcgtggc
     4321 ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcggc aatttggtgc
     4381 gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa aaaaagcaaa
     4441 cgattatccg gcgatcatag cggctttttc aaccgaagtt tgctgctttt ttctctttta
     4501 ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac gttatcgtcg atgtgcaaga
     4561 ttgcagcgtt atcgagccgt ataccgttct ggattgcgat aaaaaatcgt ctgagatgac
     4621 aaaaatattg cggctaaaaa gtatactttt acactggcga agaaacgtcg gtgacataca
     4681 attaaagcat caacaccaac cggaacctcc accacgtgct cgaatgaggt gtgttgacgt
     4741 cgggggaaac cctcctgtgt tccagcggga tagagagaaa gacaaagacc ggaaaacaaa
     4801 ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga taattccaaa agagctcgat
     4861 cagtcccctc gccccttcgg ggagagggtt agggtgaggg gaacaggcag caccagtgca
     4921 aatatttacc ctcaccccgg ccccatagtt aacgatgagt cagcttgtcc ttgtggcgct
     4981 ttagttttgt tcatcttcca gcaagcgtgc gccggtacct tcttctccta agcggtcgcc
     5041 cgggttacgc aacgggcaat cactgcgcga aaggcagcca caaccaatac atccgtccag
     5101 ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc aactcttctc gccattggga
     5161 cgaaagctgt ttccactctt tcgcacttaa cgtatgccct tctggcaaca cgccaaacgc
     5221 ttcaccaatg gtcgccagcg gaatgccaat acgctgagcg attttgataa ttgcgacata
     5281 tcgcaacaca tcacgtttat atcgccgctg attgccgctg ttacggatac tagtaatcaa
     5341 ccctttgctt tcatagaaat gcagcgccga taccgccaca ccgctgcgtt tcgccacttc
     5401 gccaggggtt agcagcgctt taatgcgggg taatttcttt tccataaatc gctttacctc
     5461 aagttaactt gaggaattat actccccaac agatgaatta acgaactgaa cactgaaaag
     5521 aggcagattt atgtcccatc agaaaattat tcaggatctt atcgcatgga ttgacgtgca
     5581 tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa tcaggctatt caaagtggta
     5641 cttgcaacgg atgttccgca cggtgacgca tcagacgctt ggcgattaca ttcgccaacg
     5701 ccgcctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt ttgatatcgc
     5761 aatggacctg ggttatgtct cgcagcagac cttctcccgc gttttccgtc ggcagtttga
     5821 tcgcactccc agcgattatc gccaccgcct gtagttttat tgcccgcgcg ttaactcccg
     5881 cgcgggtaat tgctccatcc attgcataaa cacctgcggc ggcatcgcct tcgcaaaaaa
     5941 ccatccctgg caatagcgca cgccgcgttt gcgcagccag ttaacctgct cctcagtttc
     6001 gacgccttca gcgatcgttt ttaaccccag gctgtgcgcc agctcgatga tgtgttccgc
     6061 aatcaaatga ctggttttgt gggtggtcag cgtttcaaca aacgatttat cgattttcaa
     6121 aatatcgaca ttcaatgatt taaggttatg caagttagag tagccaatac caaaatcatc
     6181 aattgccact tcgtaaccag cctggcggaa agccagaatt aatcggcgtc attttttcga
     6241 catcaagaaa tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt
     6301 gctctgtttt ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca
     6361 ggttaataga aacatagcga tcggcatgtg ttgccaggta cgcgcccaga tcgcggaaga
     6421 cattatcaat aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa
     6481 taaactctgc cggattcatt atttgcccct gctcaccagg ccaacgtaac aacgcttcag
     6541 cgccgataca tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat
     6601 gtttttcgag ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta
     6661 gccagagtaa tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat
     6721 gattataaaa atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc
     6781 gttttgtcga ataaattgtc gcatataaat agccatttct ttgtacggtt aaatccttca
     6841 aatgaatcag cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt
     6901 tcatcaccgt atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc
     6961 agaagagagg gttgataacc gccacataat ttccccgctg catataggtc attttatagc
     7021 cagagaaaaa aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat
     7081 cggccggtgc aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt
     7141 tatcacgaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     7201 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     7261 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     7321 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     7381 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     7441 caatttcctg cctgtttata cccggccatt gcaaggtgaa accctggcta ccgacgtctt
     7501 tagataaatc ttacggtata aatagcactt ttgcctgatt tgaaattgat acctttttaa
     7561 tcgtcacctg gatgaaaata attcattgat attcaaaatt aattttctac aggaatacag
     7621 aggcggcggg aagatatgcc cttaatgcca ctgaaaaacc acataatttg tacagcaata
     7681 aaagtcacgg cctaatatgg ctaacaaaca atcaattgtg atatagttca caaaattaat
     7741 gaaacaaaca gagtgtttca tttttgtgtt ctgcaagccc cgcgatagcc agagtgtacc
     7801 acgcctcccg tgaacaacgc cgcgctgtcc agggcatcgg ctcttttcag aggaattgtt
     7861 tgatggctac cctcaccact ggcgtggttc ttcttcgctg gcaacttctt agtgccgtaa
     7921 tgatgtttct ggccagcaca ctcaacatcc gttttcgtcg gtctgattat gtcgggcttg
     7981 cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg gggttgcttg
     8041 gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg atgatagaag
     8101 agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta gaa
//
LOCUS       DAZPAB010000170         8072 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZPAB010000170 DAZPAB010000000
VERSION     DAZPAB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8072
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.170"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1..1331
                     /gene="yjeM"
                     /locus_tag="ACHUPG_003277"
                     /pseudo
     CDS             1..1331
                     /gene="yjeM"
                     /locus_tag="ACHUPG_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="ACHUPG_003278"
     CDS             complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="ACHUPG_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR0862779.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFVSGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4815..5783)
                     /gene="asd"
                     /locus_tag="ACHUPG_003279"
     CDS             complement(4815..5783)
                     /gene="asd"
                     /locus_tag="ACHUPG_003279"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR0862780.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="ACHUPG_003280"
     CDS             complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="ACHUPG_003280"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR0862781.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7027..7572
                     /gene="orn"
                     /locus_tag="ACHUPG_003281"
     CDS             7027..7572
                     /gene="orn"
                     /locus_tag="ACHUPG_003281"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR0862782.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7783..7858
                     /locus_tag="ACHUPG_003282"
     tRNA            7783..7858
                     /locus_tag="ACHUPG_003282"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7816..7818,aa:Gly,seq:gcc)
BASE COUNT         1843 a         2083 c         2192 g         1954 t
ORIGIN      
        1 ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat ctattcctgg
       61 atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg gttttcctct
      121 tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc aacattcctc
      181 tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac gcaggtggtt
      241 ggtctgctgg cagtagcatg gatgattctg gtcacagtcg ttgcttcaaa ggggattaat
      301 aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa tttagtattg
      361 ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca ggatattaat
      421 ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc gtttgtggta
      481 tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga taaaacggaa
      541 aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat ttcaatcggt
      601 tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt attaagtaat
      661 ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg ggtgatgctg
      721 ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg gtttgcgcgt
      781 attaccggac tttcgatgtt cctcgcttat accggtgcgt ttgctattca ccgctgaaag
      841 ccatcatcca ggggacgccg aaagcgttgt ggccggaacc gatgacgcgc ctgaatgcga
      901 tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt ggttactgtc ttcattctgc
      961 tggtttcgtt tggtggcggt accgcatcgg cgttctttaa caagctgacg ctgatggcga
     1021 acgtgtctat gacgcttcct tacctgttcc tcgcgctggc tttcccgttt tttaaagcac
     1081 gtcaggatct cgacagacca tttgtgattt tcaaaacgcg tatgtcggca atgattgcga
     1141 cggtggttgt cgttctggtg gtgacatttg cgaacgtctt caccattatt cagcctgtgg
     1201 ttgaagccgg agactgggac agcacattgt ggatgattgg cggccctgtc ttcttctcgc
     1261 tgttagcgat ggcgatttac cagaactatt gcagtcgcat ggcgaataaa cctgagttag
     1321 ctctcgactg atcatatgaa atcctgcaac tcatcgtctc tccggcgatg agttgtcagg
     1381 aagtaatgcg tatggatgat actctccagg gccggtgata actgaagatg atgttaagct
     1441 gctaaatgag ccacgttgac atcatttggc agcttcatgc attacaaact tcccgcctga
     1501 cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt taccgttgag gctttccaga
     1561 cgcatctgga agggcgggaa tggcatatcg ataccgtggg cgtggaagcc tgccagaatc
     1621 agctggtgga tctcatggcg tagcggcata cggtgaccca tctcagcggc gtaaatacgc
     1681 agctcgaaaa tctgaatccc ctgttgcaga tccaccagga agacttccgg tgccgggttg
     1741 tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga tttccgtcac ttcttcactg
     1801 ttggcatcgg caggggccgg aatcgtcaac actacgcgcg tgaccgagtc agagagcgac
     1861 cagttgataa actgctcggt aataaacgcc ttgttcggca cgattatctc tttacggtcc
     1921 cagtcgctga tggtggtggc gcgggtgtta atttttgtca cgctaccagt aagatcgcgg
     1981 atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca ggatgatcag gccagagaca
     2041 aagttggcga aaatttcctg caaaccaaaa ccgagaccaa caccgagcgc ggcaaccagc
     2101 cactgcaatt tcgaccactc aataccaatc attgagaagc cgaccagccc gccaatcagc
     2161 atcagcagat attttgtgat ggtggtgatg gcgtaacccg tacccggcgt taaatccagg
     2221 tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc gcaccagctg cgtggtgatg
     2281 ataaatacca gaatggcaat cagcaccgca ccgagggtaa ttggctccag actttctacg
     2341 ccctgtaccg tggaggtgac atcccacagc gaaatatttt cgaggaagcc gaaagcggaa
     2401 tggatttctg accacagcac aatgaccgaa agcagggcga tcaacatcaa aattgagcgc
     2461 accagccgca aggattgcgc actgatggca tcgagatcga cttcgctttc atcgacttca
     2521 attgctcctt ccgggctact gtgatgatgc gcttcctctt cgccacgcgc acgttgcgct
     2581 aacatctctg cccggcgatg cttcgcccgg tcaaacgcca gcctgcgacg ctggatcagc
     2641 atccagcggc ggataacgtg ataaaccacc agtagcagga accagatggc aaccgaggtt
     2701 tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac ccaccgccga cgccagaatg
     2761 gcaaccagtg gtgcgccaat catcatgttc cacagcatat ggttggtaat gttgtcgccg
     2821 ctgccttctt tgttgagata caacggaata ccggcttttt tcaggcttag ggtgaccacc
     2881 gccagcgcac cgcaaatgag gataaagcaa agccgtccca gcgaaccgga aaattcgcgg
     2941 tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc cgatgctcat caggtagtag
     3001 cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat gagcgataaa caagccgttc
     3061 gggcgggcaa aggtggcgca aatcatcact acccacagca gcggcacggt ggccgttaca
     3121 ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct cgcgcaagcc gtaacccagc
     3181 gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg accagaaaag agtgcgcaac
     3241 gtcagccaga agtgatcctg agtcactttg ccgactttcg ccgccgaacg ttcaagaaaa
     3301 cgggtgaaat agcggcgcga gtaaatactg caaccgacca gaatcaacgc gccaaacagc
     3361 ggcaaaattg tctctttgct ggtcagcatc atcacactgg ctttgcccaa ctgactgaag
     3421 gtgtccagcg aaatgagacg acgcagatcc tgggcgattt ccagcggcca ggcgatggtc
     3481 atcgggcgca cgtcagaggc ccagaacaga tagcggtgcg ttgcttcgtt cacctctttc
     3541 agcgcatcct ccagttgcct gttggagact ttcagcttgg tcagttccaa tagtagcgtg
     3601 tcgccaccct gcaacaatga gttcagcaac tcacgctgag tgcgcagttg tgcttccaga
     3661 atacggtttt gctcggcggt cagcggctga ccgtcggcct gatggatttg ccgtagcagc
     3721 ggctgtttat taagtaaatc ctcataacgt aaccgttgca cacgcaactg cgccatttcg
     3781 gtatcaagct gttgtggttt tggcatttct ggtagccgtg ccacctgcgc ccgcagcgct
     3841 tcgccgagca ggttggacga tcccagccat tgcgactgtt cacgcagcgt attcaacgcc
     3901 tgccggacct gtaacgtctg gctggcagcc tgacgctgtt gcgaggcaac gagctccatc
     3961 cgctgcgcct gttgattcag cgctgccgat agttcgcggt taattttgaa ttgcgcgacg
     4021 atatctttcg gcaaatcggc actgttttct gccagtaatt cggtactttc cagcgcccgc
     4081 tccgcctcaa gctgacgttg gctattcaac tgattacgta aggcctgcaa atacgcatcc
     4141 agttgctggc tctctttttc cgccagctct gagcgtaagc gcgctaattc ctggcggtta
     4201 ttggcagaca gctgcgccag ctccagttca tcaacgagcg ccttaagacg tgcagagtca
     4261 gactgcaacg cgaaattttg tgcctgattg agcggagtat tgccggtaag cgttcccagg
     4321 cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt gctgttgcgg cagttgattc
     4381 agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct gggcctgacg gcttttatcc
     4441 agcaactggc tgctgacctg gagaatttcc tgattcagcg cgtcggtaga cattcccggc
     4501 gacacgctgc gcggctcgtc acgaatgttg tttaattgtg cgcgcagagt agcggagagt
     4561 ttcggataat tatcgataac ttgctgatat tgtttgatgc gctcaaggga accttttcgt
     4621 tcctcaagcg catttaaggc agactggagc gcctctacga cttccggctg tgcgggtttc
     4681 gccgcttttg cctgctccag ttcctgagtg atttgtttgc tatcgggggc cgtcgcggcg
     4741 tacgcccccc aactgaggca ccaggccatc agaaaagtga taatcaggcg cacgtcagcg
     4801 tttcctttga tggattagac ctggtctttt ttatcgtcaa ccaatgggct ggcgtcgtgt
     4861 tctgcttcga tctcttcagc aggaagtggg gcgggttcag cgtctggcgt aacaaaggtt
     4921 tcggtagata ctgccagcgg ctggccaatt ttcgtgacag acaggctttc cagttgctca
     4981 accagattca ctttacccgg tgcaaacagg ttgataacgg tggaaccgag tttaaagcga
     5041 cccatttcct ggcctttcag cagtgccaca gaaccgtcgt tttccccggc aggccaggtc
     5101 cagcgcttga tgataccttc gcgcggcggc gtaatggtgc ccgcccagac cgtctcaatg
     5161 ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg ggccaaattc ggtatcgaaa
     5221 aggcaaatca cgcgttcgtt acgggcaaac agatttggca cgttctgagc ggtgagatgg
     5281 ttaacggaaa agagatcgcc cggcacgtag atcatctcac gcagaatacc gttgcacggc
     5341 atgtgtacgc gatggtagtc acgcggggag aggtaagtgg tcacaaacgt accgttgcgg
     5401 aacaggtccg ccatcagata gttgcctgcc agcagggctt cgaggctgta gttgtggcct
     5461 ttggcttgca ggattttatc ttcttcgatt ttacccaact ggctgataac gccatcggca
     5521 ggcatgacca gtacattcgg atcggtatcg attgggcgta cttcgtcacg cagcggacgg
     5581 acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg gcttttgcgc ctctttcatg
     5641 tcgaccttgt agtatttaac gaacagatcg ataaccagtt ttgtcagcca tcctgcccgc
     5701 ttgcttgcgc cccaacccgc caggcgagta agccatagtt tcggcagaat gtactgtagc
     5761 gaaagtttaa atgaatttaa caaggtagcc tccaggccat tgttttgtcg ttcctgatcc
     5821 ggccaacatg ccggatcctg aaaaaaaggg gacgattcta acgacggtta gcttaattgt
     5881 cagtcatccg tatcagaaaa gtttttacgc gtttttacct gcgccatgct ttccagaata
     5941 cggtgatagt tttcgaaacg ggtttccgcg attttccctt cctcaaccgc ttcccggata
     6001 gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt tacacagacc taaatagtca
     6061 tggaattcga caaagccctg agtgatttgt tccggctcca ggtgccagag gccgaactca
     6121 cgcacaccag gggaatcaat cacatcaccg ccgtgcggga agtgatacag ccgagcggcg
     6181 gtcgtggtgt gctgaccgag ccccgagttg tcagagacat cgttggtcag gatctctttt
     6241 tgcagcccca gcaacgcgtt cagcaggctg gatttaccga cgccagactg cccggcaaaa
     6301 atgctgatgc gcccggtcaa cgcctcttcc agcggtttca gcccatcctg agtatggctg
     6361 gaaaccatca atacgcgata accgatgttg cggtagatat ccatctgctc gttgacgaac
     6421 gccatgcctt cgtcgtccag caggtctatc ttgttgagca cgataatcgg ctcaatctgc
     6481 aaggtttcgc aggccaccag gtaacggtcg ataatattga gcgacagctc cggcaaaatg
     6541 gcggagacaa tgacaatctg gtcgatgttg gcggctatgg gtttcacgcc gtcgtagaaa
     6601 tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt ccacgatccc tttgacattt
     6661 acgccttccg ccgccggttt acccggacgc cagactacgc ggtcgccggt taccagcgaa
     6721 cggatggtac ggcgaatatt gcagcggtga acgtcgccat cggcggattc cacatcagcg
     6781 tgcataccaa agcggctgat gacgatacct tcatcaggct cgccaaacag attgtcgtcg
     6841 tagtcgggct tctccttaga cgttttaaga cgacgctggt gattggcgtt cacgcggcgc
     6901 tgctggcctt tggagagttt atttttactc aatcgtactg actcctggtc gcccctgatg
     6961 ggcaaaacat ctatgataca cgcaattgtg gatcaatata gtcactgtga atgggtggaa
     7021 aatagcatga gtgccaatga aaacaacctg atttggatcg atcttgagat gaccggtctg
     7081 gatcccgagc gcgatcgcat tattgagatt gccacgctgg tgaccgatgc caatctgaat
     7141 attctggcag aagggccgac cattgcagta catcagtccg atgaacagct ggcgctgatg
     7201 gatgactgga acgtgcgcac ccataccgcc agcgggctgg tagagcgcgt gaaagcgagc
     7261 acgatgggcg atcgggaagc tgaactggca acgctcgaat tcttaaaaca gtgggtgcct
     7321 gcgggaaaat cgccgatttg cggtaacagc atcggacagg accgtcgttt cctgtttaaa
     7381 tacatgccgg agctggaagt ctacttccac taccgttatc tcgatgtcag caccctgaaa
     7441 gagctggcgc gccgctggaa gccggaaatt ctggatggtt ttaccaagca ggggacgcat
     7501 caggcgatgg atgatatccg tgaatcggtg gcggagctgg cttactaccg cgagcatttt
     7561 atcaagctgt aaaatttttc ctggtcacgt aagcgccgga atccacttgc ggcggcgtga
     7621 ttttgacgct aaatcgaacg tcttgctgat aatttcagca cttgagataa aaacgcaaaa
     7681 aaaacttttt tggggggttg cagagggaaa gatttctcgt ataatgcgcc tcccgtaacg
     7741 acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt acgcgggaat agctcagttg
     7801 gtagagcacg accttgccaa ggtcggggtc gcgagttcga gtctcgtttc ccgctccaaa
     7861 atttgaaaag tgctgcaaag cacagaccac ccaatcgcag tatttcgaat tgcgacaagg
     7921 cggcaactga acgaatcctt gggaacttac aaaagtatgt gactaaggtg agtgaaggca
     7981 gccaaggcag tagcggttcg aaagacgaag attaagcggg aatagctcag ttggtagagc
     8041 acgaccttgc caaggtcggg gtcgcgagtt cg
//
LOCUS       DAZPAB010000171         7980 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZPAB010000171 DAZPAB010000000
VERSION     DAZPAB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.171"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            101..190
                     /gene="kdpF"
                     /locus_tag="ACHUPG_003283"
     CDS             101..190
                     /gene="kdpF"
                     /locus_tag="ACHUPG_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR0862783.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            190..1863
                     /gene="kdpA"
                     /locus_tag="ACHUPG_003284"
     CDS             190..1863
                     /gene="kdpA"
                     /locus_tag="ACHUPG_003284"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR0862784.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1886..3636
                     /gene="kdpB"
                     /locus_tag="ACHUPG_003285"
                     /pseudo
     CDS             1886..3636
                     /gene="kdpB"
                     /locus_tag="ACHUPG_003285"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            3942..4514
                     /gene="kdpC"
                     /locus_tag="ACHUPG_003286"
     CDS             3942..4514
                     /gene="kdpC"
                     /locus_tag="ACHUPG_003286"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR0862785.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4507..7187
                     /gene="kdpD"
                     /locus_tag="ACHUPG_003287"
                     /pseudo
     CDS             4507..7187
                     /gene="kdpD"
                     /locus_tag="ACHUPG_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            7184..7869
                     /gene="kdpE"
                     /locus_tag="ACHUPG_003288"
                     /pseudo
     CDS             7184..7869
                     /gene="kdpE"
                     /locus_tag="ACHUPG_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
BASE COUNT         1651 a         2087 c         2348 g         1894 t
ORIGIN      
        1 ttttatactt tttttacacc ccgtccgcag atttttgcga aatctttgca gccagaattc
       61 tactcttccg gtatcacttt taggccactg gaggtgcact gtgagtgcag gcgtgatagc
      121 cggcgtgttg ctggtgtttt tattactggg ctatttggtt tatgccctga tcaatgcgga
      181 ggcgttctga tggctgcgca agggttctta ctgattgcca cgtttttact ggtattaatg
      241 gtgctggcgc gtcctttagg cagcgggctg gcgcggctga ttaatgacat tcctcttcca
      301 ggtacagcgg gcgttgaacg tatactcttt cgtttacctg gcgtctctga ccatgagatg
      361 aactggaagc aatatctttg tgccattctc ggcctgaaca tgctggggct ggcggtgctg
      421 ttttttatgt tgctcggtca gcactatctg ccgcttaatc cacagcagtt gccggggctg
      481 tcgtgggatc tggcgctgaa taccgccgtc agctttgtca cgaataccaa ctggcaatct
      541 tatagcggtg aaaccacgtt gagctatttc agccagatgg caggcttaac ggtacaaaac
      601 tttctttctg ccgccagcgg gattgcggtg atttttgcct tcatccgtgc gtttactcgc
      661 cagagcatga gcacactcgg gaatgcctgg gtcgatctgc tacgcatcac gttatgggtg
      721 ctagtccctg tggcgttgtt gatcgcactg ttttttattc aacaaggtgc gcagcaaaac
      781 tttctgccct atcaggcagt gaataccgtt gaaggagcgc aacagctgtt acccatggga
      841 ccagtagctt ctcaggaagc gatcaagatg ctcggtacta acggcggtgg cttctttaat
      901 gccaactcgt cgcatccgtt tgaaaaccca accgcactga ccaacttcgt gcagatgctg
      961 gcgatcttct tgatcccaac agcgctgtgc tttgcctttg gtgaagtgac gggcgatcgc
     1021 cgccaggggc gcatgttgct gtgggcgatg tcagtgattt ttgtcatctg cgtaggcgtg
     1081 gttatgtggg cagaagttca gggtaatcct catctgctgg cgctcggcgc agacagcagc
     1141 atcaatatgg aaggtaaaga gagccgtttc ggcgtgctgg tcagtagcct gtttgcggtc
     1201 gtgactacgg cggcgtcctg tggcgcggtg attgcgatgc atgattcgtt taccgctctc
     1261 ggcggcatgg tgccgatgtg gctaatgcaa attggtgaag tggtgttcgg cggtgtcggt
     1321 tctggtcttt acggcatgat gctgtttgtc ctgctggcgg tgtttattgc cgggctgatg
     1381 attggtcgta caccggaata tctgggtaaa aaaatagacg tacgcgagat gaaactgact
     1441 gcactggcga ttctggttac tccaacactg gtactgatgg gcgcggcgtt ggcgatgatg
     1501 accgacgccg gacgtagcgc catgcttaac cctagcccgc atggttttag cgaagtgctg
     1561 tacgccgtgt cgtccgccgc taacaacaac ggcagcgcct ttgccggatt aagcgccaac
     1621 tctccgttct ggaactgttt actggcgttc tgcatgtttg tcggtcgctt cggggtgatt
     1681 atcccggtga tggcaattgc cggttcgctg gtgagtaaaa agagccaacc cgccagctcc
     1741 ggcacgctgc caacgcacgg cccgctgttt gttggcctgt taatcggcac cgtgttgctg
     1801 gttggcgcac tgacctttat ccctgccctg gcgcttggtc cggtggcgga atatctctcc
     1861 tgatgatatt gagtgagcac tgaatatgag tcgtaaacaa ctggcgctat tcgaaccaac
     1921 acttgtcgtt caggcgctga aagaagcggt gaaaaaatta aacccgcagg cgcaatggcg
     1981 caatccggtg atgtttatcg tctggatcgg cagtctgctg accacctgta ttagcatcgc
     2041 gatggcaagc ggtgcgatgc ctggcaatgc gctgtttagc gcggcaatta gcggctggct
     2101 gtgggtcacc gtactgttcg ctaatttcgc cgaggcgctg gcagaaggcc gcagtaaagc
     2161 gcaggccaac agtcttaaag gggtgaaaaa aactgccttt gcccgcaagc tgcgtgagcc
     2221 gaaatatggc gctgcggcgg acaaagttcc tgccgatcaa ctgcgtaaag gtgacatcgt
     2281 gctggtagaa gctggcgata ttatcccctg cgatggtgaa gtcattgaag gcggcgcatc
     2341 ggtcgatgaa agcgccatca ccggggaatc ggcaccggtg atccgtgaat ccggcggcga
     2401 ttttgcctcc gttaccggcg gcacgcgtat tctttctgac tggctggtga ttgagtgtag
     2461 cgttaacccc ggtgagacat ttctggatcg gatgatcgcg atggtggaag gcgcacagcg
     2521 acgcaaaaca ccgaacgaga ttgccctgac tatcctgctg attgctctga ccatcgtctt
     2581 tttactggca accgccacgc tgtggccgtt ttccgcgtgg ggcggtaatg ccgtgagtgt
     2641 aacggtactg gtggcgctgc tggtctgtct gatcccaacc actattggtg gcctgttgtc
     2701 agcgatcggc gtcgccggga tgagtcggat gctgggcgcg aatgtgattg ccaccagcgg
     2761 acgtgcagtt gaagcggcag gtgacgttga cgttctgcta ctggataaaa ccggcaccat
     2821 cacactcggt aaccgtcagg cgtcggagtt tatccccgcg cagggcgtgg atgaaaaaac
     2881 gctggctgac gccgcacaac tggcttcgct ggctgatgaa acgccggaag gtcgcagtat
     2941 tgtgatcctc gccaagcaac gttttaacct gcgcgagcgc gatgtgcagt cgctccatgc
     3001 cacctttgta ccgtttactg cgcaaagccg gatgagcggg atcaacatcg acaaccgcat
     3061 gatccgtaaa ggttctgtcg atgccattcg ccgccatatt gaggctaacg gcggtcactt
     3121 ccctgccgat gttgatcaaa aagtcgatca ggttgcgcgt caaggagcca cgctgctggt
     3181 ggtggtggaa ggttctcgtg tgctgggcgt tattgcgctg aaagatatcg tcaaaggcgg
     3241 tattaaagag cgcttcgccc agctgcgcaa atgggcatta aaacggtgat gattaccggc
     3301 gataaccgtc tgactgccgc cgcgatagct gcggaagcgg gtgtcgatga ttttctcgcc
     3361 gaagcgacac cggaggccaa gctggcattg atccgtcagt atcaggcgga aggtcgtttg
     3421 gtagcgatga ccggcgacag caccaacgat gctacggcgc tggcgcaggc agatgtcgcg
     3481 gtggcgatga actccggcac ccaggcggct aaagaggcgg gcaatatggt ggatctcgac
     3541 tctaacccga ccaagttgat cgaggtggtg cacattggca aacagatgct gatgacccgt
     3601 ggctcgctga ccaccttcag cattgccaac gatgtggcga aatacttcgc cattattccg
     3661 gcggcgttcg cggcaacata tccgcagtta aatgcgctga acatcatgcg cctgcattcg
     3721 cccgactccg ccattctcag tgcggtcatt ttcaacgcct tgattatcgt ctttttgatt
     3781 cctctggcgt taaaaggcgt gagttataaa ccgcttaccg cttctgccat gttgcgccgt
     3841 aacttatgga tttacggtct gggtgggctg ctggtgccgt ttattggtat caaagtcatt
     3901 gatttactgc taaccgtttg tagtctggtg tgaggtttac catgagtgga ttacgtccgg
     3961 cattatcgac atttctcttt ctgttattga ttactggcgg cgtttacccg ctgctgacca
     4021 ccgcactggg gcaatggtgg tatccctggc aggccaacgg ttcgttgatt cgtgaaggtg
     4081 atacggtgcg cggttcggca ttaatcgggc aaaattttac cggcaacggc tattttcatg
     4141 gtcgcccgtc ggcaacagca gaaatgccct ataatccaca ggcttctggc gggagcaatc
     4201 tggcagtcag taacccggag ctggataaac agatagccgc acgcgttgcc gcattacggg
     4261 ccgctaaccc ggacgccagc acgaacgttc cggttgaact ggtgacggcg tcggcaagcg
     4321 gactggacaa taacatcacc ccgcaagcgg cggcctggca gatcccacgc atcgcaaaag
     4381 cgcgtaatct cagcgttgaa cagctcacgc agctgatcgc aaaatacagc caacaaccgc
     4441 tggtgaaata tatcggccag ccggtcgtca acattgtcga actcaatctg gcactggata
     4501 agcttgatga ataacgaacc cttacgtccc gaccccgacc gtctgctgga acaaactgcc
     4561 gcgccgcatc gggggaagct gaaagttttc ttcagtgcct gtgcaggcgt cgggaagacc
     4621 tgggcgatgc tggcagaagc ccagcgactg cgggcgcaag ggctggatat tgtggttggt
     4681 gtggtagaaa cccatgggcg gaaagatacc gccgccatgc ttgaagggct ggctgttctg
     4741 cctctgaaac gccaggcgta ccgtgggcgg catatcagcg agtttgatct cgatgccgcc
     4801 ctcgcccgcc gtccggcgct gatcttaatg gacgaactgg cgcacagtaa tgcgccaggt
     4861 tctcgtcatc ctaaacgctg gcaggatatc gaagaactgc tggaagctgg cattgatgtt
     4921 ttcacaaccg tcaacgttca gcatctggaa agtctgaatg atgtggtcag cggcgtcacc
     4981 ggaattcagg tacgggaaac cgtgcccgat ccttttttcg atgccgccga cgacgtggtg
     5041 ctggtggact tgcccccgga cgatctgcgc cagcggctga aagaaggcaa agtctatatt
     5101 gccgggcagg cggagcgcgc cattgaacat tttttccgca aaggcaatct gatcgccctg
     5161 cgcgaactgg cactgcgccg tactgccgat cgcgttgatg agcaaatgcg cgcctggcgg
     5221 gggcatcctg gcgaagagaa agtctggcac acgcgcgacg cgatcctttt atgcatcggc
     5281 cataacaccg gcagcgaaaa ctggtccgcg cggcggcgcg gctgacgtca cggctgggga
     5341 gcgtctggca cgcggtgtat gttgaaaccc ctgccctgca tcgcttaccg gagaaaaaac
     5401 gtcgggcaat tctcagcgcc ttacgtctgg cgcaggagct gggcgcggag acggcaacac
     5461 tttctgatcc agcggaagag aaagcggtag tgcgttatgc ccgtgaacat aatctcggca
     5521 agattattct cggtcgccca gcctcgcgcc gctggtggcg tcgggaaaca tttgctgacc
     5581 gactggcgcg catcgccccc gatctcgatc aggtgctggt cgcgcttgat gaaccacccg
     5641 cccgcacgat taacaacgcg ccggatagcc gctcatttaa agacaagtgg cgggtacaga
     5701 ttcagggatg cgtggttgcc gccgcgttat gcgccgttat caccttaatt gccatgcagt
     5761 ggctgatggc gtttgatgcc gccaacctgg tgatgctgta tctgcttggc gtggtggtgg
     5821 cgctatttta tggacgctgg ccttcggtgg ttgccaccgt cattaatgtg gtgagtttcg
     5881 atctcttttt tatcgcccca cgcggcacgc tcgccgtctc tgatgtgcaa tatctgctga
     5941 ccttcgcggt gatgttaacc gtcgggctgg tgatcgggaa ccttactgct ggtgtgcgtt
     6001 atcaggcgcg ggtagcccgt taccgcgagc aacgcacacg gcacttatat gaaatgtcga
     6061 aagctctggc ggtgggccgc agtccgcagg acatcgccgc caccagcgaa caatttattg
     6121 cctccacgtt tcatgcccgc agtcaggtgt tgttgcccga tgacaacggt aaattgcagc
     6181 cgttaacaca tccgcaagga attacgccat gggacgatgc catcgcgcag tggagttttg
     6241 ataaaggcct gcctgcgggc gcgggcaccg acacgttacc cggtgtaccg taccagattt
     6301 tgccgctaaa aagcggcgag aaaacctacg ggctggtggt ggtggagccg gggaatctgc
     6361 gccagttgat gatcccggaa cagcagcgcc tgctggagac ttttacgctg ttagtcgcca
     6421 atgcccttga gcggctgacg ctaaccgcca gcgaagaaca ggcgcggatg gcaagcgaac
     6481 gtgaacagat ccgcaacgcc ctgctggcag cgctttcgca tgatttacgc acgccgctta
     6541 cggtgctgtt tggtcaggca gaaatcttaa cgctcgatct ggcaagcgaa ggatcacccc
     6601 acgcccgcca ggccagcgag attcgtcagc atgtgctgaa cactacccga ctggtgaata
     6661 atctactgga tatggcgcga attcagtccg gcggctttaa tttgaagaaa gagtggttaa
     6721 cgctggaaga agtagtcggc agcgcgctgc aaatgccgga accgggttta tcgtcgccca
     6781 tcaatctttc tctgccagaa ccgctgacct taatccacgt tgacgggcca ctctttgaac
     6841 gggtgctgat taatctgctg gagaacgcgg tgaaatatgc gggtgcgcag gccgaaattg
     6901 gtatcgatgc ccacgttgag ggcgaaaatc tacaactgga tgtctgggat aacggccccg
     6961 gtcttccgcc aggccaggag cagacgatat ttgataagtt tgctcgcggg aataaagagt
     7021 cggcggtgcc gggtgtcggg cttgggttgg caatttgtcg ggcgattgtg gatgtacacg
     7081 gcggcaccat taccgcgttc aaccgaccgg aaggtggtgc ctgttttcgt gttacacttc
     7141 cccagcaaac cgctcctgaa cttgaagact ttcatgagga tatgtgacaa acgttctgat
     7201 tgttgaagat gaacaggcta ttcgtcgctt tctgcgcacg gcgctggagg gcgacgggat
     7261 gcgcgtcttt gaggccgaaa ctctgcaacg cggcttgctg gaagcggcaa cccgtaagcc
     7321 agatttgatt attctcgatc tcggcctgcc cgatggtgat gggattgagt ttatccgcga
     7381 cctgcgccag tggagcccgg tgccggtgat tgtgctttcc gcacgcagcg aagagagcga
     7441 caagatcgcc gcgctggatg ccggagcgga tgattatctg agtaagccgt ttggtattgg
     7501 cgaattgcag gcccgtctgc gcgtcgcttt acgccgccac tccgccactc cgccaccgcc
     7561 gcgcccgatc cgctggtgaa attttccggt gttaccgtcg atttagccgc ccgcgtgatt
     7621 caccggggtg aggaagaggt gcatctcaca ccaattgagt tccgcctgct ggcggtgctg
     7681 ctcaacaatg ccggaaaagt actcacccag cgccagctcc ttaaccaggt gtgggggcca
     7741 aacgcggtcg aacacagtca ctatttgcgt atttatatgg gacatctgcg acaaaaactg
     7801 gaacaggatc cggcccggcc tcgccacttc attactgaaa ccggtattgg gtatcggttt
     7861 atgctttgaa ttgttattca ataaattcgc ttaaatagtg attaatttat tattaaacct
     7921 gcgccgaata ttattatttg gcaggtttaa tttcttaacg caaatataaa acacgaaatt
//
LOCUS       DAZPAB010000172         7938 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZPAB010000172 DAZPAB010000000
VERSION     DAZPAB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7938
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.172"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            259..762
                     /gene="dps"
                     /locus_tag="ACHUPG_003289"
                     /gene_synonym="pexB"
     CDS             259..762
                     /gene="dps"
                     /locus_tag="ACHUPG_003289"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFR0862786.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1166..1912
                     /gene="glnH"
                     /locus_tag="ACHUPG_003290"
     CDS             1166..1912
                     /gene="glnH"
                     /locus_tag="ACHUPG_003290"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR0862787.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            2051..2710
                     /gene="glnP"
                     /locus_tag="ACHUPG_003291"
     CDS             2051..2710
                     /gene="glnP"
                     /locus_tag="ACHUPG_003291"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR0862788.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2707..3429
                     /gene="glnQ"
                     /locus_tag="ACHUPG_003292"
     CDS             2707..3429
                     /gene="glnQ"
                     /locus_tag="ACHUPG_003292"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR0862789.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            3690..5914
                     /gene="ybiO"
                     /locus_tag="ACHUPG_003293"
                     /pseudo
     CDS             3690..5914
                     /gene="ybiO"
                     /locus_tag="ACHUPG_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(5911..6837)
                     /gene="rlmF"
                     /locus_tag="ACHUPG_003294"
     CDS             complement(5911..6837)
                     /gene="rlmF"
                     /locus_tag="ACHUPG_003294"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR0862790.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            7113..7373
                     /gene="mcbA"
                     /locus_tag="ACHUPG_003295"
     CDS             7113..7373
                     /gene="mcbA"
                     /locus_tag="ACHUPG_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR0862791.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            7638..>7938
                     /locus_tag="ACHUPG_003296"
     CDS             7638..>7938
                     /locus_tag="ACHUPG_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR0862792.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT         1935 a         2032 c         1980 g         1991 t
ORIGIN      
        1 tctcgctact tttcctctac accgtattta tatatcgaat tatgcaaaag catatttatt
       61 ccgaaaattc ctggcgagca gataaataag aattgttctt atcaatatat ctaactcatt
      121 gaatctttat tagttttgtt tttcatgctt gttaccacta ttagtgtgat aggaacagcc
      181 agaatagcgg aacacatagc cggtgctata cttagtctcg ttaattactg ggacataaca
      241 tcaagaggat atgaaattat gagtaccgct aaattagtta aatcaaaagc gaccaatctg
      301 ctttataccc gcaacgatgt ctccgacagc gagaaaaaag caacagtaga gttgctgaat
      361 cgccaggtta tccagtttat tgatctttct ttgattacca aacaagcgca ctggaacatg
      421 cgcggcgcta acttcattgc cgtacatgaa atgctggatg gcttccgcac cgcactgatc
      481 gatcatctgg ataccatggc agaacgtgcc gtgcagctgg gcggtgtagc tctggggacc
      541 actcaagtta tcaacagcaa aaccccgctg aaaagttacc cgctggacat ccacaacgtt
      601 caggatcacc tgaaagaact ggctgaccgt tacgcaatcg tcgctaatga cgtacgcaaa
      661 gcgattggcg aagcgaaaga tgacgacacc gcagatatcc tgaccgccgc gtctcgcgac
      721 ctggataaat tcctgtggtt tatcgagtct aacatcgaat aaatccatcg ctgatggtgc
      781 agaactttag tacccgataa aagcggcttc ctgacaggag gccgttttgt tttgcagccc
      841 acctcaacgc acttatttag tgcatccatc tgctatcccc agctgattaa gtaaattttt
      901 tgtatccaca tcatcacaca atcgttacat aaagattgtt ttttcatcag gttttacgct
      961 aaataatcac tgtgttgagt gcacaatttt agcgcaccag attggtgccc cagaatggtg
     1021 catcttcagg gtattgccct ataaatcgtg catcacgttt ttgccgcatc tcgaaaaatc
     1081 aatgagttgc aaaattggca cgattttttc atatatgtga atgtcacgca ggggatcgtc
     1141 ccgtggatag aaaaaaggaa atgctatgaa gtctgtatta aaagtttcac tggctgcact
     1201 gaccctggct tttgcggttt cttctcatgc cgcggataaa aaattagttg tcgcgacgga
     1261 taccgccttc gttccgtttg aatttaaaca gggcgataaa tatgtgggct ttgacgttga
     1321 tctgtgggct gccatcgcta aagagctgaa gctggattac gaactgaagc cgatggattt
     1381 cagtgggatc attccggcac tgcaaaccaa aaacgtcgat ctggcgctgg cgggcattac
     1441 catcaccgac gagcgtaaaa aagcgatcga tttctctgac ggctactaca aaagcggcct
     1501 gttagtgatg gtgaaagcta acaataacga tgtgaaaagc gtgaaagatc tcgacgggaa
     1561 agtggttgct gtgaagagcg gtactggctc cgttgattac gcgaaagcaa acatcaaaac
     1621 taaagatctg cgtcagttcc cgaacatcga taacgcctat atggaactgg gcaccaaccg
     1681 cgcagacgcc gttctgcacg atacgccaaa cattctgtac ttcatcaaaa ccgccggtaa
     1741 cggtcagttc aaagcggtag gtgactctct ggaagcgcag caatacggta ttgcgttccc
     1801 gaaaggtagc gacgagctgc gtgacaaagt caacggcgcg ttgaaaaccc tgcgcgagaa
     1861 cggaacttac aacgaaatct acaaaaaatg gttcagtact gaaccgaaat aataacgcta
     1921 cacctgtaaa gcgcactggc agttccctct cccccctggg gagaggatta gggtgagggg
     1981 cgcaaacccg ctccggggcc attaattacc ctgaatttga ttatttacac cacggtaaca
     2041 ggaacaacat atgcagtttg actggagtgc catctggcct gccattccgc ttctgattga
     2101 aggtgccaaa atgaccctgt ggatttcggt cctcggtctg gctggcggtc tggtaatcgg
     2161 attgctggca ggttttgcac gcaccttcgg tggttggata gccaaccacg tcgcgctggt
     2221 ctttattgaa gtgatccgcg gcacacctat cgtcgtccag gtgatgttta tctatttcgc
     2281 cctgccgatg gcgtttaacg acttacgcat cgacccattt actgcggcgg tggtcaccat
     2341 catgatcaac tccggcgcgt acattgcgga aatcacgcgt ggtgcggtgc tgtctatcca
     2401 caaaggtttt cgtgaagcag gactggcgct cggtctttca cgttgggaaa ccattcgcta
     2461 cgtcatttta ccgctggcac tgcgtcgtat gctgccgccg ctgggtaacc agtggatcat
     2521 cagcattaaa gacacctcgc tgtttattgt tatcggcgtg gcggaactga cccgtcaggg
     2581 gcaagaaatt attgccggta acttccgcgc ccttgagatc tggagcgccg tggcggtgtt
     2641 ctatctgatt attaccctgg tgctgagctt tattctgcgt cgtctggaaa gaaggatgaa
     2701 aatcctgtga ttgaatttaa aaacgtctcc aagcactttg gcccaaccca ggtgctgcac
     2761 aatatcgatt tgaacattgc ccagggcgaa gtcgtggtga ttatcgggcc gtccggttcc
     2821 ggtaaatcga ccctgctgcg ctgcatcaac aaactggaag aaatcacctc cggcgatctg
     2881 attgtcgatg gcctgaaggt caacgatccg aaagttgacg agcgcctgat tcgccaggaa
     2941 gcaggtatgg tgttccagca gttttacctc ttcccgcatc tgacggcgct gggaaacgtc
     3001 atgtttggtc cgctacgcgt gcgtggcgcg aacaaagaag aggcggaaaa actggcgcgt
     3061 gagctgctgg cgaaagtcgg tctggcagaa cgtgcacatc actacccttc cgaactttct
     3121 ggtggtcaac agcagcgtgt ggcgattgcc cgcgcgctgg cggtgaagcc gaaaatgatg
     3181 ctgtttgatg aaccgacttc cgctcttgac ccggaactgc gccatgaagt gctgaaggtt
     3241 atgcaggatc tggctgaaga agggatgacg atggtgatcg tgacccacga aatcggtttt
     3301 gccgagaaag tagcttcgcg gctgatcttt atcgacaaag gccggattgc ggaagatggc
     3361 aatccgcagg tgttgatcaa gaacccgccg agccagcgct tgcaggaatt tttgcagcac
     3421 gtctcttaat aagacacatt gcctgatgcg ctacgcttat caggcctaca ggatatctgg
     3481 caacttatta aaattgcatg aacttgtagg acggataagg cgttcacgcc gcatccggca
     3541 aaaaagcccg cacgttgtca gcaacctgct taatatccct tcctcccttt cacccgaaag
     3601 ggaggcacac cagattcctc tcatttaaaa tcgcccctcc cccagcatct atacttatct
     3661 ttttgctctg ttttctcact ggaggagtca tgcggtggat cctgttcatc ctcttctgcc
     3721 tgctgggcgc acctgcccac gcggtatcca tacccggcgt tacaaccaca acgacaacgg
     3781 actcaacgac tgaaccggcc ccggaaccgg atatcgaaca aaaaaagcgg cctatggcgc
     3841 actggcggat gtgctggata acgacacctc gcgtaaagag ttgatcgacc agttgcgtac
     3901 tgtcgccgcg acgccccctg cggaaccggt cccgaagatc gtgccgccga cgctggttga
     3961 agagcaaacc gtgctgcaaa aggtcaccga agtcagccgc cattatggtg aagccctttc
     4021 cgcccgcttc gggcaacttt atcgcaatat caccggctcc ccgcataagc catttaatcc
     4081 acaaaccttc agcaatgcgc tgagccattt ttcaatgtta gcggtattag tgtttggttt
     4141 ttactggctg attcgcctgt gcgcactgcc gctatatcgc aaaatgggtc agtgggcgag
     4201 gcaaaaaaat cgtgagcgca gcaactggtt gcagcttccg gcgatgatta ttggggcgtt
     4261 tattatcgac ctgctgttac tggccctgac attatttgtc ggccaggtat taagcgacaa
     4321 cctgaatgca ggcagtcgca ccatcgcttt ccaacaaagt ttgtttctca acgcctttgc
     4381 cctgattgaa tttttcaaag ccgtactacg cctgattttt tgcccaaacg tggcggagct
     4441 gcgcccgttc acgattcagg acgagagcgc ccgttactgg ggtcgtcgcc tgagctggtt
     4501 aagcagcctg attggctatg gcctgattgt ggccgtgccg attatctcta atcaggtgaa
     4561 tgtacagata ggtgcgctgg cgaacgtcat cattatgctg tgcatgaccg tctgggcgtt
     4621 gtacctgatc ttccgaaata aaaaagagat tacccagcat atgctcaact ttgcggagca
     4681 ttcgctggct tttttcagcc tgtttatccg cgcctttgcg ctggtgtggc actggctggc
     4741 aagcgcctat tttatcatgc tgtttttctt ttcgttgttc gatccgggca acagtctgaa
     4801 atttatgatg ggtgcaacgg tgcgcagcct ggcgattatt ggtatcgcag cgtttgtttc
     4861 cggtatgttt tcccgctggc tggcgaaaac catcaccctc tcgccacata ctcagcgtaa
     4921 ctatccggag ctacaaaaac ggctgaatgg ctggctttcg gcggcgctga aaacggcgcg
     4981 tattctgacc gtctgcgtgg cagtaatgtt gctgttgagc gcatggggat tgttcgattt
     5041 ctggaactgg ctgcaaaacg gcgcggggca gaaaaccgta gatatcctga tccgcatcgc
     5101 actcattctt ttcttctcgg cggttggctg gacggtgctc gccagtttga tcgaaaaccg
     5161 gctggcttcg gatattcatg gccgcccgct acccagcgcc cgtacgcgta ccctgctgac
     5221 gctgttccgt aacgcgctgg cggtgattat cagtaccatc accatcatga ttgtgttgtc
     5281 ggaaatcggc gtcaatatcg cgccattgct ggcaggtgcc ggggcattag gtctggctat
     5341 ctcgtttggt tcgcaaacgc tggtaaaaga tattatcacc ggggtattta ttcagtttga
     5401 aaacggcatg aacactggag atttggtgac tatcgggccg ttgaccggca cagtggaacg
     5461 gatgtcgatt cgctccgtgg gcgtgcgcca ggataccggg gcgtatcaca tcattccgtg
     5521 gtcttcgata accacctttg ctaacttcgt ccgcggtatt ggttcggtag tggcaaacta
     5581 cgatgttgat cgccatgaag atgctgataa agccaatcag gcactgaaag atgcggtaac
     5641 ggaattgatg gaaaacgaag aaattcgcgg gctgattatt ggtgaaccga actttgccgg
     5701 gattgtcggc ttaagcaata ccgcgtttac actgcgtgtt tcgttcacca cgctgccact
     5761 caaacagtgg acggtacgct ttgccctcga cagccaggtg aaaaaacatt tcgacctggc
     5821 gggcgttcgc gcgccagtgc agacttatca ggtgctgcct gctccgggcg cgaccccggc
     5881 tgaaccgctg ccgccggggg agccaacgct ttaacgttgg cgattaacaa aacggcggcg
     5941 ctgctcgtcg ttcataaagg tccaggcaat aaagcgactc tgcttttgcc cctgggccat
     6001 ctcttttttt accaccttca ccgcgcccac gtcggtcaga gcacgataca acggcggtaa
     6061 gttttcacca cgagatacca gcgaggtaaa ccacatcacc tgcttcgcga agcctttgct
     6121 ctcttcaatc atctttttga taaagttgac ttcaccgcct tcacaccaca actcctgttg
     6181 ctgaccacca aagttcagtg catcgtcttt gttcagcccc aggttacggc gtttacgctc
     6241 actacctgcc cgcgccgcag cggcggaatc gtggaatggc gggttacaca aggtcgcgtc
     6301 gtattgctcg tttttgtgga tgatgccgtt aaaaatcgcc ccactctctt tttgccgacg
     6361 cagacgaatg gcgcggttaa gccccggatt agcactgata atcgcctgcg cactgcttaa
     6421 cgcctggctg ctggtttcac taccggtaaa acgccagcca tattcatgta cgccaatcag
     6481 cggataaata cagttcgcac caacgccgat atccagaatg ctggcattcg ctggaattgt
     6541 tccgcttgct tcagccagta aatcggcaag gtgatgaata taatccgccc ggcccggtac
     6601 tggtgggcag agaaaaccgt cggggatatc ccagttcgct acggcgtaaa aatgggccag
     6661 caacgcctta ttgagcgcct tcaccgccag cggattggca aagtctacgc tttgctcccc
     6721 tgcgggtgta agcgtgagga attgcctgag ttcaggattg acctgacaaa gcgtggcgag
     6781 atcgtagcgg ctgtgatgac ggttgcgcgg atgcaacccc ggtttctggg cggacatagc
     6841 attctccttt aagcagcggc gtaagatacc cgcataacgc gacagggtaa atatctggct
     6901 gcgtttatca ttccccaaaa ccttaacatg gctatggttt atggcaattc atcataaaaa
     6961 tcagcattat gcatgtcatt gatttgtctt attttgtaaa aatatatcgt tcaaaaaata
     7021 agcatttctc tgtcaatttt ttgcgtcatt tgcatcgcgc aggcgaccta cacttatcag
     7081 gcactacccg ttgtatttat gaggatgtca ttatgaaaaa gtgcctcaca ctactgattg
     7141 ccaccgtcct gagcggaatc tctctcacgg cttatgccgc gcaaccgatg agtaacctgg
     7201 acagcggtca gctacggccc gccggtaccg tttcggcaac tggcgcatca aacctaagcg
     7261 atctggagga taaactggca gaaaaagcgc gcgaacaagg cgcgaaaggt tatgtcatta
     7321 attccgcagg cggaaatgac cagatgttag gtactgcaac catctacaaa taaccccacc
     7381 aacgtaactg cattcgcctc tggatgcagt tttcgcctgt tttgtaatca aaaaacatca
     7441 aatatgaacg caatgtaaat aaatgtattt ctttttcgcg caatgggtga tagaaaatcg
     7501 catcaagtga taatgcttat caaaattatt atcacttgca cgaacactat cacgggatta
     7561 acagtggcat cgcatccgca gagatgcttt ctcgtggcag tgaaaatttc aacatataag
     7621 aaaaagtcac ctgcaaaatg gaaaacaatc gcaatttccc tgccagacaa tttcattcgc
     7681 tcacgttctt tgccggtctt tgtattggta ttacgcctgt ggcacaggca ctcgccgccg
     7741 aagggcaagc taacgcggat gacacgctgg ttgtcgaagc atcaacgcct tcgctttatg
     7801 cgccacaaca atctgccgat ccgaaattct cgcgtccggt agcggatact acccgcacga
     7861 tgacggtgat ttctgaacaa gtgattaaag atcagggcgc aaccaacctt accgacgcgc
     7921 tcaaaaacgt ccccggcg
//
LOCUS       DAZPAB010000173         7934 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZPAB010000173 DAZPAB010000000
VERSION     DAZPAB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.173"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..956)
                     /locus_tag="ACHUPG_003297"
     CDS             complement(<1..956)
                     /locus_tag="ACHUPG_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFR0862793.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="ACHUPG_003298"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="ACHUPG_003298"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFR0862794.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="ACHUPG_003299"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="ACHUPG_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="ACHUPG_003300"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="ACHUPG_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR0862795.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="ACHUPG_003301"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="ACHUPG_003301"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR0862796.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="ACHUPG_003302"
     CDS             complement(5018..6346)
                     /locus_tag="ACHUPG_003302"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR0862797.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..7934)
                     /locus_tag="ACHUPG_003303"
                     /pseudo
     CDS             complement(6354..7934)
                     /locus_tag="ACHUPG_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616724.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
BASE COUNT         2102 a         2010 c         1789 g         2033 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctgatgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaagca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttaacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgc
//
LOCUS       DAZPAB010000174         7721 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZPAB010000174 DAZPAB010000000
VERSION     DAZPAB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.174"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..832)
                     /locus_tag="ACHUPG_003304"
     CDS             complement(<1..832)
                     /locus_tag="ACHUPG_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0862798.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     E"
     gene            1014..2438
                     /gene="sbcB"
                     /locus_tag="ACHUPG_003305"
     CDS             1014..2438
                     /gene="sbcB"
                     /locus_tag="ACHUPG_003305"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR0862799.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2481..2708)
                     /gene="tsuB"
                     /locus_tag="ACHUPG_003306"
     CDS             complement(2481..2708)
                     /gene="tsuB"
                     /locus_tag="ACHUPG_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR0862800.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2722..3780)
                     /gene="tsuA"
                     /locus_tag="ACHUPG_003307"
     CDS             complement(2722..3780)
                     /gene="tsuA"
                     /locus_tag="ACHUPG_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR0862801.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3959..5317)
                     /gene="plaP"
                     /locus_tag="ACHUPG_003308"
     CDS             complement(3959..5317)
                     /gene="plaP"
                     /locus_tag="ACHUPG_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR0862802.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5307..5369)
                     /gene="yoeI"
                     /locus_tag="ACHUPG_003309"
     CDS             complement(5307..5369)
                     /gene="yoeI"
                     /locus_tag="ACHUPG_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR0862803.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5530..6513)
                     /locus_tag="ACHUPG_003310"
     CDS             complement(5530..6513)
                     /locus_tag="ACHUPG_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0862804.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6559..7383)
                     /locus_tag="ACHUPG_003311"
     CDS             complement(6559..7383)
                     /locus_tag="ACHUPG_003311"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0862805.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7466..7720)
                     /gene="yoeB"
                     /locus_tag="ACHUPG_003312"
     CDS             complement(7466..7720)
                     /gene="yoeB"
                     /locus_tag="ACHUPG_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR0862806.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2067 a         2012 c         1812 g         1830 t
ORIGIN      
        1 gttcgaagat tggacggcgg ataacattac ccatcttgct atgctctttc ggtacggtcc
       61 atacgttgtc cagcaggtca aattctgtct ttgttgccag cctaagctct gagagcctcg
      121 ccccccacag cataagcatc tgatgaagga gcttatttga cgtagacgca cggcttcttt
      181 caatagcaag ccaaatctta gccagttcgt gatacgacag tacccgatcc cctacctcag
      241 cgcgggagcc gaagtccctt ggctggatgc tcataattgc gcaactatct atcaactgac
      301 gccgcatgca ccaactaatt gctgatctta gttgacttag cacctgcctt gctcggcgtg
      361 gattatcttt ttcttcttcg gtaagcaggt caacccattg cttaaccgtg atagaagatg
      421 ccggacgatt agggaaggcg tcatgcatgc gcttcataac cgttgatcga taaagtgcct
      481 gggtcttttc tctgagagtt gtagagacgt agttgtcgaa ccagtagtcg agacactggg
      541 cgaccgtcat ggagttctcc accttctctt caaaataggt gcgtggatcc gttcctgaga
      601 aatagagctt tcgcaagtca gcagtgatct gtctggcatc cttcaaagac agggatgggt
      661 atcgaccaag cccaagtcga ttaggcttgc catgccagcg atagcggtac tggaactgga
      721 tgaccccctt cggtgaaatt cgtacgctga ggccatcggc atcagccact tcttgtgggc
      781 ccgaatatgg tttaccataa atagtacgca gttttgtgtc gcttatagcc ataaataata
      841 tttggtacgc tcagaaataa tagtttggca cacatctggt acacaatatc acatggacaa
      901 cagcgcacag taaacaacta tatttgacgt tcgtagacat actcaggtga aaaaagggtt
      961 gcttttcgag ttataacaga cagttaatta actacagtgg acaatgctag acaatgacag
     1021 acacagataa acaacctacc ttcctctttc acgattacga aacctttggc acgcaccccg
     1081 cgttagatcg ccctgcacag ttcgcagcca ttcgcaccga tgacgaattc aatgtcatcg
     1141 gcgaacccga agtcttttac tgcaagcccg cggatgacta tttaccccgg cctggagcag
     1201 tattaattac cggtattacc ccgcaggagg cacgggcgaa aggagaaaac gaagccgcgt
     1261 ttgccgcccg tattcactcg ctttttaccg taccgaagac ctgtattctg ggctacaaca
     1321 atgtgcgttt cgacgacgaa gtcacacgca acgtttttta tcgtaatttc tacgatcctt
     1381 acgcctggag ctggcagcat gataactcgc gctgggattt actggatgtt atgcgtgcct
     1441 gttatgccct gcgcccggaa ggaataaact ggcctgaaaa tgatgacggt ctaccgagct
     1501 ttcgccttga gcatttaacc aaagcgaatg gtattgaaca tagcaacgcc cacgatgcga
     1561 tggctgatgt gtacgccact attgcgatgg cgaaactggt aaaaacgcgt cagccacgcc
     1621 tgtttgatta tctctttacc catcgtaata aacacaaact gatggcgttg attgatgttc
     1681 cgcagatgaa acccctggct cacgtttccg gaatgtttgg ggcatggcgt ggcaatacca
     1741 gctgggtggc accgctggcg tggcatcctg aaaatcgcaa tgccgtaatt atggcggatt
     1801 tggcaggaga catttcgcca ttactggagc tggatatcga cacattgcgc gagcgtttat
     1861 ataccgcaaa agccgatctt ggcgataacg ccgccgttcc ggttaagctg gtgcatatca
     1921 ataaatgtcc ggtactggcc caggcgaata cgctacgccc ggaagatgcc gaccgactgg
     1981 gaattaatcg tcagcattgc ctcgataacc taaaaattct gcgtgaaaat ccgcaagtgc
     2041 gtgaaaaagt ggtagcgata ttcgcggaag ccgaaccgtt tacaccttca gataatgtgg
     2101 atgcacagct ttataatggc tttttcagtg acgccgatcg tgcagcaatg aaaattgtgc
     2161 tggaaaccga gccgcgtaat ttaccggcac tggatatcac ttttgttgat aaacgaattg
     2221 aaaagttgtt gttcaattat cgggcacgca acttcccggg gacgctggat tatgccgagc
     2281 agcaacgctg gctggagcac cgccaccagg tcttcacgcc agagtttttg cagggttatg
     2341 ctgatgaatt gcagatgctg gtacaacaat atgccgatga caaagagaaa gtggcgctgt
     2401 taaaagcact ttggcagtac gcggaagaga tcgtctaatt tgaagccgcc gccgctggta
     2461 cggcggagga caatgttgag tcaggctttt tgaacggtga tgctccacgc tgcatcgcca
     2521 atctgctgat aatcggtgat ggcatgtcct tcttctgccg cccactgtgg gattgcttca
     2581 gttgcctggg tgcaatcaaa ctcaattacc aactcatcgc cgctggccat ttctgccagt
     2641 gctgcttttg cttctatgag tggaaacgga cagacctgcg tcaccacatc cagcttttta
     2701 atcgccatct ttacacctta cttaatttgc cgcagcagtt gccagtcgcg ccttacgctg
     2761 cggtcgaaca tacacaagcc aggacgcagt ccagactccg agaatcataa atacaaggcc
     2821 aatccagccc tgccaggtca tcattgcagt cataaccagt ccattaccga tagagcaacc
     2881 acctgcaata ctggcaccga agcccatcag tacaccacct aacccgctac gtaatgttgt
     2941 ttgtgcatca gctgcgcgaa cgcggaactc acggctcgct ttggctgcaa taaatgcctc
     3001 cacgaagatc cctaacacca ggaaaacgcc ccagttaatg tatttcacgt cacccgcgac
     3061 cagaaattga agaatattgg ccgttgggga agtgactcct aacccaaaca tgcgtccggt
     3121 tgcttcactc aggggccagg ctaaaagcgc aatcaaaccg atgagtacag cagtgacaaa
     3181 gggatgccag cgtttttcaa acagaatatg agcgatcccg gttcggcgcg gcggtaaggt
     3241 cgcgactttg agttttggct tcttcaactc tttcatcacc acccagagcg ttatcaccag
     3301 caaaacggta accaacggcc acacagacaa attaaaagtc tcagcaatag agttatgttc
     3361 agtagtgtag tgctgcaagg tttgatttaa accactggcg tgtggagaac gcatcaccgc
     3421 actcatcacc atataagtga aaagcgcgat ccaactgccg atcaatccct cacccgcgcg
     3481 ataccaggtc ccggtggcac atccacccgc cagaacaatt cccagcccga aaagataccc
     3541 acctataaca gtaccaagcc acgggaacgc cccggcttcg taagtcagta ggcccgcctg
     3601 aattaacgca aaaaccccca cgctttgtgc cgaaattgca atcagcaagg cgtaaaacat
     3661 gcgattattt ttcgcgatat acatatcgcg aaaaccacca gtcaggcaaa aacgcccacg
     3721 ctgcatgaca aatcccagca gagcaccaca aattagcccg cttaatatca ttgaaaacat
     3781 aaattaatta accagatgaa tgttaatggg gaaatttttc atgactggtg gaatgcagac
     3841 caataaccaa attctctaaa ttagaacaaa tggttattaa tgaggatttc gataaaaaag
     3901 aataaaaaaa ccggagccaa atgttcattc gactccggtt tattattaga aaagatgatt
     3961 atgctacgtc ttcgtactgc ggaaccggat tacggaagct cttggtgacg caagccaggt
     4021 aaatcagacc gatagcagcc cagatcagac ccagaaccat tgagctttct tccaggttaa
     4081 cccacagcgc accaacggtc agcgcaccac acatcggcag gaacagatac tggaagtgat
     4141 ctttcagcgt cttgttacgc ttctcacgga tccagaactg cgagatgacc gacaggttaa
     4201 caaaggtgaa cgccaccagc gcaccaaagt taatcagcgc cgttgccatt accaggtcga
     4261 agttgattgc cagcagggca atcgcgccaa ccaggatgat gttcatcgcc ggagtacgcc
     4321 atttcgggtg tacataaccg aagaagcttt tcgggaacac gccgtcacga cccattacgt
     4381 acatcagacg cgctacgcct gcgtgcgccg ccataccgga cgccagtacg gtaatggtgg
     4441 agaagatcag cgcgccaacc tggaacgctt tacccgcaac gtacagcatg atttcaggct
     4501 gtgacgcatc cggatcttta aagcgagaga tatccgggaa gtacagctgc aggaagtaag
     4561 ttgcaaagat gaagatcatg ccgccaatca gcgcggtcag gaaaatcgca cgcgggatca
     4621 cgcgctctgc atctttggtt tcttccgaca gattgctgat gccgtcaaag ccggtaaagg
     4681 agaagcacag gattgtcgcc ccggtaatca tcgggataac atgcgcatcg ccagaccaga
     4741 acggacgcgt gctcgccagc gtaccagcgc cttcaccttc aaatacgcca taaacaacca
     4801 tgcccagaat caccgcgatc agcactacct gcaacacgac gattacagtg ttgacgttcg
     4861 ctacggattt cagactacgc aggttaaagg cggtcataaa ggccaccagc gccaccacaa
     4921 acatccacga tggaatggaa ggcaccagag cttcaaaata gattttcgcc agcagaatgt
     4981 tgatcatcgg cgcgaacaga tagtcgagca gagaagacca acccaccata aagccgacag
     5041 tcgggctaat ggatttctgg gcgtaagtgt atgcagagcc agcagaagga tagcggcgaa
     5101 ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat caatgcgaac gcataggctg
     5161 tcggcacatg accatccgtg aggcctgaaa cgataccaaa tgtatcaaac agcgtcatcg
     5221 gctgcatata ggctagaccc atcattacaa ccggaactaa cgtaagcgtt ttacgcaatt
     5281 ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt tattctcctt tacggtgata
     5341 accgtcgcgt aagcaaaaaa ttgccccatt tttttggatt cctcagcgac aacaactgtc
     5401 gatttttagt aaatatctat ccggtacgaa gcccggcctc ttggtatgaa tgattggttt
     5461 gaagcaaaaa ataaccgacg ctaattaaac gtcggttttt aatcattttt tgacagcggc
     5521 gcattgtgcc taaatggggg ggaaatgaca agagaatgag aggcttgtca taataatttt
     5581 tctttgaatg gctgattttc cgtcatcaga ttttgcataa acaccggcga tcgcactatt
     5641 tgctaaaatt tcatctcgcc accagccgct ggcaagccct gcggtctgtt cattccagcc
     5701 aatccagaca ggttcaaaga gagtttgggc gaccactttc ttctcgatta acgcgccact
     5761 atcgagaaaa cgttgcgcca gataccgtgg taaatagccg cagccaaggc cgctaatttg
     5821 cagctccagt ttggttttaa aatcgaaaac ggtaatggct tcttgttcgt cgagaagttg
     5881 tgaggccgta gaagcgcctg cctgcgcagt atcccccacc acaatcgccc gatatcgttt
     5941 aatgatacga cggtttaacg gttcttcttc ctgggctaac ggatgatgag gcgcgaccgc
     6001 aaagacttgt tcaagatcgc ccaaccgcga gaaaccaaac tcactggaag atggtggctc
     6061 atgcatcgcg ccaacgataa tatccgctcg cccctgagtc agggcatccc aggaaccacc
     6121 gagtacgcca ttgataaact tcaggcgcgt cacgctatga tgttgataaa aagcttcaat
     6181 gagaggcgca agcagagaaa aaggaaacgt gtcgtccacg ccaatcacca gctcgttttc
     6241 ccagccttca tgcagtttga tcgcctgctt ttccagttct cgcacggtat gcagaacttc
     6301 ccgccctttc tctaataaca tttttccggt gcgggtgaat ttagcgcggt gtccgctacg
     6361 atcaagcaat tgaatattta ggtcgctttc cagcttgtga acggtgtaac tcagagcgga
     6421 cggcgtcttg tagagtttgg ctgacgccgc cgcaaaactg ccctcttttt ctatggcatc
     6481 gaggatcatc aacacatcca gcagtggttt catacacgcc cccttgcagt gcatgcggcg
     6541 tcctgctggc gtgatcgttt actccaacgg cattaccagc ggatcgggat actggtattc
     6601 aaatcccagt tcattacaaa tccgactgcc atcaataatc ttacctttgc cgctgtccag
     6661 actatttctg aactgcggag gttgcagccc cagtaaacgg gccatctgcg gatagaaaac
     6721 attacgcgca gggtgagctg gcgcacatat attatagatg tgtccgcctt taggtgcctg
     6781 taacaacaga gtgatagcgc caataacatc ttctaaatgg actaaattaa caccatgttc
     6841 accatcaggg gcggtttttc cggcaaagaa gcgtcccgga tgacgtcccg gtccgaccag
     6901 gcccgcaaga cgcagaatat cgaccgaagt accgggtaaa ttgtgcagcc agtcttcgag
     6961 ttcttctaat actcgtccac tgttggttac cggattacgc ggggtagttt ctttaaccgt
     7021 gccttgcgcg tcgccataga cagatgttga gctggtaaaa ataattcggg gaatacgatg
     7081 ggccagcgcg ctatccacta actcttgtac cgcttgtaaa tagaactcat cgccggggcc
     7141 gctacgacgt gccggaagcg taatgaccag cgcatcggca tccataaggg catccagatc
     7201 gtcagaatcg caaactaact caggctccat gcgaagcaga tagctatcaa tgccactcat
     7261 tcgggccgct tcgacaccat cttgtgtggt tttactcccg gtgacttgcc agcctcgcgc
     7321 tgaaagtgac atcgccagcg gcatacctaa ccaccctaac ccgacaattg cgaccttttt
     7381 catccgttat ctcctgatac accttagatc tataaggcta cgctagcgta tcaaaactga
     7441 caattcattc tatgaatgaa tctgttcaat aatgataacg acatgctgca atgagcagtg
     7501 aatcatcagt aaccgcgtat accagacggt gctcctctgt aatgcgtcgg gaccagaaac
     7561 ctgacaaatt atgtttcagg ggttctggtt tccccttacc ttcaaatggt gttctgcggg
     7621 tatctttgat aagttcattg atctttttaa caattcgctt atctgtttcc tgccagtaca
     7681 gataatcatc ccatgattcc tcagaccaga ttagtttcac t
//
LOCUS       DAZPAB010000175         7565 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZPAB010000175 DAZPAB010000000
VERSION     DAZPAB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.175"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(88..1559)
                     /gene="yeeO"
                     /locus_tag="ACHUPG_003313"
                     /pseudo
     CDS             complement(88..1559)
                     /gene="yeeO"
                     /locus_tag="ACHUPG_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            1720..1795
                     /locus_tag="ACHUPG_003314"
     tRNA            1720..1795
                     /locus_tag="ACHUPG_003314"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1753..1755,aa:Asn,seq:gtt)
     gene            complement(1833..2783)
                     /gene="cbl"
                     /locus_tag="ACHUPG_003315"
     CDS             complement(1833..2783)
                     /gene="cbl"
                     /locus_tag="ACHUPG_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR0862807.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2885..3802)
                     /gene="nac"
                     /locus_tag="ACHUPG_003316"
     CDS             complement(2885..3802)
                     /gene="nac"
                     /locus_tag="ACHUPG_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR0862808.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4130..4205
                     /locus_tag="ACHUPG_003317"
     tRNA            4130..4205
                     /locus_tag="ACHUPG_003317"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4163..4165,aa:Asn,seq:gtt)
     gene            complement(4261..5196)
                     /gene="ldtA"
                     /locus_tag="ACHUPG_003318"
     CDS             complement(4261..5196)
                     /gene="ldtA"
                     /locus_tag="ACHUPG_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR0862809.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(5258..6337)
                     /gene="cobT"
                     /locus_tag="ACHUPG_003319"
     CDS             complement(5258..6337)
                     /gene="cobT"
                     /locus_tag="ACHUPG_003319"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR0862810.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6349..7092)
                     /gene="cobS"
                     /locus_tag="ACHUPG_003320"
     CDS             complement(6349..7092)
                     /gene="cobS"
                     /locus_tag="ACHUPG_003320"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR0862811.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7089..>7565)
                     /gene="cobU"
                     /locus_tag="ACHUPG_003321"
     CDS             complement(7089..>7565)
                     /gene="cobU"
                     /locus_tag="ACHUPG_003321"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFR0862812.1"
                     /translation="SSQVLYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDEL
                     IHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQR
                     CPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2086 a         2048 c         1639 g         1792 t
ORIGIN      
        1 ggcgaatatt agcgatgccc acccacaatg tcaaagcctg ttttttaaat ttgaaatcgt
       61 ttgctgaaat aatctgcatt ttgtcgttta ttccgacaca actggctttt tttcacactt
      121 ttgcggctcg gatcgagggt atttccacag ccaacgtcca gtaaccattc gccagtaaaa
      181 cagcacggcc cgcacagccc agtcggcaaa cattcccatc cagacaccaa ccacacccca
      241 gccaagcatg attccctgca cataaccgac tacaacccgg caaccccaca tgctcaacat
      301 agaaacccac atggcgtaac gggcatcacg agcaccctta aatccagcgg gtagcaccca
      361 tgaggcggac caaataggca taaataaagc atttagctaa atcagaatca caacgacgtg
      421 tttaacctgt ggatcctggg tgtaaaacga tgccataacc ccggcaaagg gagccgttag
      481 ccaggcgatg gccgttaatc caagagtgga cagccagaac acatgccgca actgaatctc
      541 tgcttgcgct atctgcccta cccccaacct tcggcctgta atgatcgtag aagcagaacc
      601 gagcgcactt ccgggtaagt tgataagagc cgcaattgag aacgcgataa aatttccggc
      661 aataacgctg gtccccatcc cggcaacgaa catttgggtt aataaccgac cactggtaaa
      721 taacactgat tcgacactcg ctggaatacc aatccccatg acttcccaga taatgctaaa
      781 attcagcggt ttaaaatagc tctttaacga aatccttagc gcaggattaa aaccaatcgc
      841 cagcacccac aaaattgcaa ctgcgccaat ataacgagaa atggttaaac ccaacccagc
      901 cccgaccagg agaaaaggcc gtaaatcaat atgccgctaa taataatatt aagaatattc
      961 aggctaccgt taatcagtag cggtattttc gtattccctg caccacgaag tgccccgcta
     1021 ccaataagag tgatggcagc ggctggataa ctgagtacgg tcagctccag ataagtcaac
     1081 gccagtgctt taacttctgt cgtggcatca cccgcgacga aatcaataat ttgttcgcca
     1141 aaatgatgaa ttagcgttgc caacagtacg gcaaacaacg tcatgatcac caatgactgc
     1201 cgcgtcgcca ccctcgctcg tcgtcgatcc cgcttaccga gactaaatgc tacaacgaca
     1261 gtagtaccaa gatcgatagc tgcaaaaaaa gccataatga ccatattgaa gctgtccgcc
     1321 aaccccacgc cggccatcgc atcttttccc agccaactga ccagaaaagt gctcaggacc
     1381 cccatcaaca ggacacaggc gttctccatg aagataggaa cagcaagcgg ggttatctcg
     1441 cgctagaaca acactttgta gctcttgcgt ttagagtgcc agcgagtgcc gtgaacaacc
     1501 tggcgtaaag cagaggagat attcaaagcc gaccttaatt gcagaaagtg aaaccacatt
     1561 tcaaatgatg agggagaatc agcaaagctg caaagatttt cgccaacaaa ttgtctgcaa
     1621 atgcaacaaa ctgttgatag aaacggcaaa cagttgggaa atttaaaaat cgggtttgac
     1681 aaaatatttt tcgccgttaa gatgtgcctc aacagcgatt cctctgtagt tcagtcggta
     1741 gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattt
     1801 ctgaaaagcc cgcttttata gcgggctttt tgctatatct gataatcaat ttcctcttca
     1861 ctgttttcca tcacctggcg cttgatatcc tcaactgaca gtcctgcatt acaaagttcc
     1921 agaaagcgcc aaacatagtt acgctgaagt tgtcctcgct tcagtcccaa ccagacagta
     1981 ttagcatcga aaagatgccg cgtatccagg cggattaaat tctcttcctc ttgttcgccg
     2041 ctggattgtt cggcaactaa tccgatccca agcccaagag caacataggt tttaatgaca
     2101 tcagaatcct gtgcacttaa tacaatatct gccggcaaac ctttgcgggt aaatgcgtca
     2161 tcagtgcgtg agcgccccgt aattccctgt cggtaagtaa ttaacggcca cttcgctatt
     2221 gattccagcg tcaatggtgt aatttgcgtt aagggatgat cgagtggaac aagcaaacta
     2281 tggtgccaac aaaaccatgg gaaggcgacg agctgcgggt cattactcaa acgctcgctg
     2341 gcgataccaa tatcagcttc gccattttgc aacaacgtcg caatttcctg tggcgaaccc
     2401 tggattagct cgagccgaac ctccgggaaa agttcgcgaa aagctttaat gacctctggc
     2461 aagctataac gtgcctgagt atgcgtcgtt gcaatagtga gaacaccaga cgtatcgttg
     2521 gtaaacaggt ctgcaagccg acgaacatta ctggcttcat tcagaatacg ttctgcaatg
     2581 accagtaatg ctttgcccgg ttcagtcatc cccagcagtc gcttacctcg tcgaacaaat
     2641 atttcgatgc caaattcatc ctccagttcc cgaatatgac ggctaacgcc tgactgtgag
     2701 gtaaaaagca tattcgcaac ctctgtcagg ttgtaatcct gacgtgcagc ctcgcggatt
     2761 atctttagtt gttggaaatt cacggtaaac tccgggcagt tcagatatcc cgttattgtt
     2821 aaagtctaat gcccggcata acaaataata aaaacccgca tcttattcca tcccgatata
     2881 acgcttagct caccaattgc cactgccttt tttccatcac tggagaacta atcactgaca
     2941 ttaacaactc tttcactgcc tgtgcctgtg gtgacaagtt tgctctggcg ggtaaattta
     3001 aggacaaaga gagactcatt gaaggagtgg taatgcgtga catccaccca tttactgcgc
     3061 cacataacga acgcgcagcc gattcaggta atactgcaac gcccatgccg ctggcaatcg
     3121 ctgcggtaag cgtggcaata gactcaattt caccaatgac ttttgccgtg agccgccgta
     3181 gggaaaaagc ctcatcaaca cgaagtctaa cagcgctgta atcactgggg agaaaaaggt
     3241 tcatttgcgc aatagcatta acatcaacgt tttgccccgg gcaatcttga gttcctacca
     3301 ggaaaagatc ttctttcagc aaagcctgac tggatacccc agccacaggg gaatgctcat
     3361 aaatcaccgc catatcgagt tggtgattta tcagtttttc gttaagcact gcaccactat
     3421 tttcatgaag atagataacg acctccggga attcagcgcg aaccgcctgt aataagggca
     3481 tggtgatgga tgacgcagcg gttcctggcg caaagccaat cgagacttgc cccgataatg
     3541 actgaccaac gttatgcacc gccagttggg cctgctcaca ctgacgtaaa atggcccgtg
     3601 catgggtata gagaattttt ccggcgtctg ttggcgtaac gccccgtttt gtgcgaatca
     3661 aaagctgttg atttaactca ccttccagta tggcaacctg ttggctgagc gctggttgtg
     3721 cgatatgcaa tacttcagca gcctgggtca ggctaccaat atctacaatt tttacgaagt
     3781 atttcaggcg tctgaagttc atgttgcctc cggtttttaa gaatcggccc aagtgccgcc
     3841 attacttaca accagtatgc aagatgcctg ccagttctat tttggcgttg atgcgcaagc
     3901 taaccaactg tcaaataaga gattatgata gattcgtcat tggccctttt tatcagcagt
     3961 cgcattcacc tgccctataa aaggagggta tgcaccacga tggttcatta cccaataaga
     4021 ttgaaagctc accactttgt tgaaattggc agcaaacaaa caaaaaaaat gcatttcacc
     4081 ctttgacatc accatgcact gccattaata tgcgccccgt tcacacgatt cctctgtagt
     4141 tcagtcggta gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg
     4201 agccaaattc aaaaaagcct gctttcgagc aggcttttta ctttctaatt accaacgctc
     4261 ttaaaacatc tgtcttgaac cagaactaat ttgcacaggc attcccgatc gatgttgcaa
     4321 cgcagcattt gcgcgattta catcaacttc ttgcccgttg ataaacgccc gcaaagatgg
     4381 ggttaccggc aatggcactt ttcggtcaga ctcatattct gcacgattgc gcgacagcgg
     4441 ctcatgaact tccagccact ttgaaccatc cggttccgtt gtgtatttca ctggctgatc
     4501 aataatttgt acacgagtgc ctacaggaac attatcaaac agatatttga tatcgtcatt
     4561 acgcagacga atacagccct gacttacacg gagcccaata ccaaaattgg cattggtacc
     4621 gtggatggca tacaacctgc caatataaat cgcgtacagc cccatggggt tatcgggccc
     4681 cgcaggaaca aatgcgggca aactctcccc tcgtttcgca tattcgcgcc ggatgttcgg
     4741 cgttggcgtc caggttggtg cttcttgttt acgttcaacg gtagtcaccc agttacgcgg
     4801 ggtttctcgc ccagcctggc cgataccaat aggaaagact tccactgtat tactgtctgg
     4861 cggatagtaa taaagacgca tttcagcgac gttaaccaca atccctttac gaacagtggc
     4921 gggcaaaatc agttgctgcg gaatggtgag ttgtgagcca ggcttcggca aaaaaacatc
     4981 agcgcccgga ttcgcttcca gcatattact taacccttgc ccgtattgtg cggcaaaagt
     5041 ctccagcggc tgagtattgt gatcaggaac agttacagta agcgactgcc ccactaaacg
     5101 gctaccctct ggaggtaatg gataagttac cgccaggcta ttttggctgg caaaaagcag
     5161 agcaaatgag caaagaatat ttacacgacg catcatgtcc ctttcctaag tcgcgaatac
     5221 tatccgataa gtatagcttt tatcagactt ttcgttttta gctgttcaaa tcagaagtcg
     5281 tattccccgg tagaacaata ttactggcag caagttcgcc catgttgttg tatatcgcac
     5341 aagcagcttc gatgatggac atcgccagag ctgcaccact cccctcacct aaacgcatat
     5401 ccatattgag ataaggctcc agccccaaat gcgagagcgc tatacgcgcg cctttttctg
     5461 ccgacaagtg agaaggaatg agatacggtt tgattgcagg agacatctgg caggctgcga
     5521 gcgcagcagc ataagaaaga aatccatcca gcaacacggg taaaccgcag gaagcagcac
     5581 ctaacatcac tccagctatt ccgaccaaat caaatccacc cacttttgcc aggacatcaa
     5641 caccatcctg aggatttggt tgattcaacg taatcgcccg acgcacaaca tcaattttat
     5701 tagccagttt atctgtcggc aggtttgcgc caatcccaac cacttcttca ggatcccggc
     5761 cagtgattgt gctgactatt gccgctgccg gtgtcgtgtt tgccatcccc agttcaccta
     5821 caccaaacag cgtgacaccg tttttcgcca gctcccgcgt ataacatatg acgtccaaaa
     5881 gcaacttttc agcctgacgg cgactcattg ccggagctga agcaatattg ccgctacctc
     5941 gtgcgacacg catgttgata agcccgggga taggctcagc agtatcaata ccaacatcaa
     6001 ttacgtggac gttagcgccc gcttgtgctg ccagcacaca cacgccggtt gttccacggg
     6061 tcatattttc agcctgtatg gctgtcactt cttttggaga aatagcgacc ccttcctccc
     6121 agacgccgtg atcggcacac ataaccagta ccgctttttt gcccacatgc ggtatgccat
     6181 tcacccccgg cattcctgcc agttgtatgg caagcgcctc cagctttcct aggctaccaa
     6241 caggtttgaa taacccgtca acatgccgtt gtgcacgcga catagcggca gaattgatgg
     6301 ctgggatcgt attcagtaaa tcggcaatta tttgcatgtc acgtttcttt atagcagtgc
     6361 cagcaggaag accagttcac caagctctat ggctgctccc agcgtatcgc ccgtttgccc
     6421 gcccagcgta cgtttgagaa gctgaccgag gatgaaaatc gccaccatcg tcaccaccat
     6481 agcagccaca ccatgcatac ccagcaataa tacggcagca aaaatagcgg ctaagccgag
     6541 tgtgacgcag gtttgtcgcc catcaatttt gccaatgaac acattgccaa gcccttcttc
     6601 ccgcgcataa cgatgacgat acatcaataa tgcggcagtc ccgcggctga ccgcacatgc
     6661 tgccgccagc gatgcaagga tcggttcgcc acgcagagcc aactcgctta acaccagaat
     6721 ctttgccagt accacaaaaa tcaatgccag accgccgtgg gtgcctaaac ggctatcacg
     6781 catgatttcc aacatgcgat cgcgactacg tgcagaaaat acgccatcac aggtatcagc
     6841 gaggccgtcc agatggaatt ccccggtcat cagcgccagc acaagtacgc taaacagtgc
     6901 cgccagtggt gcgccacacc atgcctgcag caccatgaag accagcccgc taatcgcgcc
     6961 aagcaataat ccaatcaaag gaaaagtaat aataccgcga gaataatgtt cgaaatccag
     7021 tccctgggac cagcgacgcg gtacaggcag acgcgtaata aatgagagca tcgcccaaaa
     7081 taatttgctc atttgatttt tactccaata cccgaaacca ccagccatac ttcatttgcc
     7141 gcagcggcca actgctgatt tacccgcccg gcaatatcac gaaaatgtcg tgccagacga
     7201 ctctccggca caatccccat tcccacttcg ttagtcacta atacaacctt tgcggggcaa
     7261 cgttggcagg cagcaatcaa cgactgaatc tcagcattaa tcgcctgttc catcgcctga
     7321 taatcccatt catcagggtc tttatcgccg ccataatcaa acaacagatt agtcaccatt
     7381 gtggtaacgc attccagcaa cacagcctca ttcgggttaa tgtctgcatg aattaattca
     7441 tcaagatgtt gccagcgctc cactgtgcgc cagtgctccg ggcggctttg ccgatgatgt
     7501 tctatccgtg cagccatctc atcatcaagg atttgcgagg tagcgatata cagaacctgt
     7561 gaaga
//
LOCUS       DAZPAB010000176         7557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZPAB010000176 DAZPAB010000000
VERSION     DAZPAB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.176"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="ACHUPG_003322"
     CDS             complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="ACHUPG_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HFR0862813.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="ACHUPG_003323"
     CDS             complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="ACHUPG_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR0862814.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="ACHUPG_003324"
     CDS             complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="ACHUPG_003324"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR0862815.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="ACHUPG_003325"
                     /pseudo
     CDS             complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="ACHUPG_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="ACHUPG_003326"
     CDS             complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="ACHUPG_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR0862816.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNCIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="ACHUPG_003327"
     CDS             complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="ACHUPG_003327"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR0862817.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6042..7181)
                     /gene="wza"
                     /locus_tag="ACHUPG_003328"
     CDS             complement(6042..7181)
                     /gene="wza"
                     /locus_tag="ACHUPG_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR0862818.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
BASE COUNT         1756 a         1997 c         1908 g         1896 t
ORIGIN      
        1 acctaaatcg cccagacggg tggaaatata aggaaacgcg attaccacac caacccggaa
       61 tacagccaga gaaatcaatg ctaacggtaa ttttttctta atcgcctctt tattcagata
      121 ttgaaatgcc cactccagca aataaaacag gataaaggtc ataacccctg aaaacgatcc
      181 ggataatatt atccctgcga gaatcagggc atctgtttta ggcgttttga taccaaactg
      241 tttgatgctg agccaaattg agattaatgc cagagcgaaa aatgccggtt cgaaataaag
      301 tgctgtcgtg cgcttgccgc cgaatttaat gaaattcagc acatagctgt tgctgtaaat
      361 cagatatttc gaaatcgact ccataatact gctgccaccg gtgaggataa tttgcgccat
      421 ctccaccgcc gccagcgcca ccaccagtcc gaccaccaga taaaagaaac gtaatatctt
      481 acgatggttg tgtggcgaaa ttgttttaaa acgaatactc cacaccatgc caataatgat
      541 cacaatatag acaaacagca tagttgaagt gacgtattta ccggcatcca acgactgacc
      601 aaacaggtag ttaaacgccg tgagtcccgc gccaatccct aaggcaatca tcaatttttt
      661 aatgctgatg cgttctaaaa acagcagtaa caagacaggt aaaaaagtga cgatggtgat
      721 ggggaaactt tcgccaagct gggcgatttt gacgttaacc agcaggtaga tcagtggcag
      781 cagcaggtag ctacagattc tgatagaagt tgacatactc ctccagcatc tgttgtccac
      841 tgtaggcggc gcgactgcgt tggctgaact cagccagcgt ggtaccaaat atcgcctgcg
      901 cgatttccgg tttgcttaac tgtaccagtt gcagcacctc ttcttcactg acggttttac
      961 caccggattt ttgcagcact tcccgcgccg catcgctatg ggtggcaatc accggcacgc
     1021 caatcgatag cgcctcacac aaaatcagcg ggtagttatc gacacgagaa ctgaacacca
     1081 gcgcatccat ctgattgagc gcgctcatca acttgcgctt gtcagtttca aagccgtgat
     1141 taaccacgtt gccagcggtg aacggcgaga acttaccaaa ggtatgcagt tcgattttgt
     1201 cgcccagcgc catcatctca cgcaccagtt gctggttagt tttgccgtcg taacgcaggt
     1261 catgcgccac cactgcgatt ttcggcttgc cctgagtttc gcgcaccgga ggcagatctg
     1321 ccagaatcgc ttcggttgcc atatcaatgc cattattgat aatccggcaa cgccctggcc
     1381 cgtacagact attgaaagcg tcagccacat gctggctggg ggaaataaac tgacagccca
     1441 gcgccagcat ctcgcggaat aactggcgtt tgcccgccac cagctggtgc gcgcgatcaa
     1501 tcttcaccgg cggatagtta tttaaggtcg ggcatttctg gcagcccgtt ttccagcctt
     1561 cgcaaccgtc ggtaaaggcg cagcgtccgg ttacactcca gtggtcgtgc agcgtccaga
     1621 ccagggtgac atccggttta tggtttttca ctttttcgca aaagcgcacc acgctgttaa
     1681 gattcaacca gtagctgtgc agcacatgaa aatgcaggac caccggaccc ggtgtgcgag
     1741 taatggtgcg atataactca ttgaaattgc cgaacagatc gcgattaaac agacgaaaca
     1801 acgcaatgtt cgccatcgcg gtcatccgcg gcgtatgttt gatgacctgc ggatagttct
     1861 gatggctgac gctctctttg ccgcctttgc cgtagccgta gacaaaatgt gacgccagcc
     1921 cctgttgcag cgcacgctgg tggagatcta acgccacacc tgctgccccg ccttccgcca
     1981 gtcgcacatt aaattgcaga atattcattt aatcaccttc actcgcgctt tttctcccac
     2041 caccagcgcg ttgtccggga cagagtcgag caccacgctg cccgcaccca cggtgacgtt
     2101 gttaccaagc gtgatatcac caagaataat gacgttggca ccgagttcga cgccgttgcc
     2161 aatgtgtgga catgccatgt tatcggcacc ccgattgccg atagtgacgc cgtggcgaat
     2221 ggtgaaatca tcccccgcca ccacgttttt attgatcact acggcgtaac cgtgatggat
     2281 agtaaagcgg cggccaatgg tcgcggcggc ctggatttca taaccgaaaa agcattcggt
     2341 gataatgcga tacagcacca gcagcggggc cgcccacaga ttgttgagga cgttcttttt
     2401 gcgccacacc gaacaaaaat gagcgacgcg ataggcaagt accatgcagc acgggcgtaa
     2461 actccagctg ttggcgcgca gatcttccag catctttagc gcccccgaat accatcagcc
     2521 agacgtttgc cgttacgcac cgacagcagc gtcaacaagg tgcgccaggt catacgttta
     2581 ttgcggatct ggtagagggt aaacagctga tattttttac tggcgcggtc gaatttgtct
     2641 ttgtgcttgc ggtaaaagtg gaagtatccg gagaattttt tcggtgacga ggtgatctgc
     2701 atttcgccgt gattgatatg caggatctgc gtcgcctctt ccactttcca cggttcgccg
     2761 tactccacca ccatccgcag gaatatgtcg taatcctgtg ctgctttcag ttcggtatcg
     2821 aacaaacact ctttgaaacg ccatgcccag gtaaagacct gattgccaat gatattacgt
     2881 ttatagaaca ggcggcgtga gtacggcgat ttgggataca gtggtaagct cgccggttgg
     2941 gaataaactt ctccctagca aacgtagtcg ttagcgtaca aaaaggcgtg tgtgaccagt
     3001 tgctgtttat gggcgaggaa gacgctcagg cggttgggcg tccattcatc atcatcatcg
     3061 atcccggtga tgtattcccc ttgcgccagc ataatcgcct ggttacgcac cgcgcacgcc
     3121 ccgctgttaa tgtcgttgtg gatgtaagtg atacgcggat cgttgagggc agtgacgtac
     3181 tgttgcagtt gttcccagga ggtggagcaa tcatccacga tgatcatctc ccagttgctg
     3241 tagtcctggc gcaggaccga ttttatcgcc cgaatcgcca gttgttggcg gttccaggtt
     3301 ggcatataga ttgagatcag cggattgtct ttcataggtt gttctcccgg cttatgagct
     3361 ggtttgcttg ccggatgcgg cgtgagcgcc ttatccggcc tacggagcgg tgcgaatgca
     3421 ggcgtcgtta tttcgcatcc gacttatatt cgtattcgta atagccataa tcctgatacg
     3481 cgctggcgcg gcggaagatg gagttcagaa tcaccccttt caccggaata cagttttgct
     3541 caaagcggct cagactggtt tccacttctt tcaatgtgtt gaccgcataa cgcgccacca
     3601 ttaacgtggt tccgacatga cgaccaacaa ttgccgcatc ggtcactgcc agaatcggcg
     3661 gcgtatcaat caacaccagg tcatagtttt tactcgccca gttcaccagt tcggcaaagc
     3721 gttcgctcat taacagttca gaaggatttg gcggcacctg accgcgcggg atcaggtcaa
     3781 atttggcaat agaggtcggt ttagcagcgg tagtaatatc gccctgacca atcagaatct
     3841 ccgacagccc attgacatta ttggtgccca acagctcgtg ggtgtagcct ttgcgcatat
     3901 cgcagtcgat caacaacacg cgtttattgg tctggctgat caccgccgcc aggttggcgc
     3961 agacaaaggt tttaccgatt gacgggctaa ccccggtcat catcaacaca ttgttctgcg
     4021 cctgcatcat cgcgaagtgc agactggtac gcagactacg gatggcttca atcgccagat
     4081 cggttggatt ccccactgcc agtagctggc tctgtttata gcgtttaacc cctttgatgg
     4141 ttttgacgct atcgcgcgct ttctgccact ccgacagcgg gatgctggca tagacgctga
     4201 ttccgtgttc ttccagcact tgcgggcttt cgatgccgcg attaaacaac gagcgcagca
     4261 gcacacccac gatagagagc atcaggccaa ggataatcgc gccgaggata atcagccctt
     4321 tcttcggttt tagcacgcca ggctgggtga ttgccgggtc aataatgcgc acatcgccga
     4381 cggtgctggc ttcggtgatt ttcagctcct gctctttatt cagcagttgc atatagacct
     4441 gctgaccaga ctcgacatcg cgggtcagac ggacaatttc ctgctggttt ttcggcatcg
     4501 ccgttacgcg accgttaagt ttggctttct cttcttccag cgcctgacgt ttctccagca
     4561 gcgtgcggta cgccgggtga actttggtgt acagcttgga gatttccgcc tctttaaagg
     4621 tcagttcgtt caactgcgcg tcgatgttca ccatcgaatc gagcaccgct ttcgcttcaa
     4681 gcggcagatc aacagaatct ttatcctgac ggaaggcatt cagtttgttt tcggcaacat
     4741 cgaggcggct acgtacttcc ggtaactgtt gcgcgaggaa agcgaggctt ttcgacgctt
     4801 ccgccgattt gcgctcaata ttttgttcct gatagttacg agcgatgctg ttaagaatgt
     4861 cgcggatctg ttcgcgatct tcaccggtat aagtcaggct cagtacgcct gcgtctttgc
     4921 cgttctccgt taccgtcagg ctgttttgca gttggttgat catccccaac gtggagtatt
     4981 tggtgacggt aaactcactg cccgggcggg cgtgaatggc ttcaaccatc agcgtgacgc
     5041 cttctttttt cagcatctgg cccgcttgcc cacgggcgct aaagccgcca tcactgctca
     5101 gggtgtagtt tttgtcatcc agcacattaa gcgtaaacac ctgatccgcc atctctttcg
     5161 ggcggttaaa ggtagtcact ttcaccgtct cgttctgacg ccccatcagg cgatcccagc
     5221 ccgccccgaa aatcgggaac gtgtttttgc tcaccgcaat atcgaggtcg agatcgtcca
     5281 ccgttttacc aagcaccaga cgcgaacgaa tcaactggat ctcggcgtcc gatgcaggcg
     5341 gtttgttggc taatgccgag ccgatatcct gcaccagcga attgccgctg ttttgctcga
     5401 tttgtaccag tgcgtcggca ctataaatcg gcgtggcgaa gaaagtgtaa accacggcac
     5461 agagggcaaa tacgacggtg atgccaatca cccaccagcg cgcttcaatg acggtgccga
     5521 ccaggcgacc aatatcgatt tcatcactgc ccgttaccgg agcggcatgt tgttttactt
     5581 tttctgtcat tcttatacct gctctgcgtt caatgcctgc gcccactggc gggcagaccg
     5641 ttcaagtaat gtgtacaccg ctgcaaacgt ttcccggctt ttgtgatacg gatcggggat
     5701 ttcacattcg ttatcccagt gaccaaacag catcactttg ccgcgcatct ccggtgccat
     5761 ctcgcataag cgttcgatat ggcgcttatc catggtcaaa atcaggtcat agttgcgaca
     5821 caggcggcgg ctgatttgtc gtgcacagtg gccttccaga gacagttgat gttctgcggc
     5881 gacgctgata gcggtaggat cagcgccctt accgaccagc gcgccgagtc cagcggactc
     5941 cactttcagt tccgggtgat aacgttgcag taagcgttcc gccatcgggg aacggcaaat
     6001 attgccgaca cagacaacta agatgttgtt aaacatgacg attaccagtt atgaatgtcg
     6061 ctggctgtat ccgtcatgta acggacaccg ctaatagttg gcagcaactg attgatcaga
     6121 cggttccagc gggaaaccgg ggcggtggtg acatacacca cgtcataagg ttgcaggcgg
     6181 aattctgtcg ccatcaccag cgacgtcgca tcggacatat ccagctggta gatattggca
     6241 atcttgccgt tacgcccgcc ctcgcctttc agcggacgaa tgacaaagat gccgctggcg
     6301 ttggaggtgg tcatgtcgat accttcggca ttggccagag cttcagtcag ggtcatgccg
     6361 ctaaagtcca ttttcagggt gctctgtttc ttcacttcac ccatcacaaa tactttcaga
     6421 tcatcattac gcggcacgta gagaatatcg ccgggataaa gcagtcggtt ctgattgagg
     6481 tcgccgtttt gcatcagcgc ctgtaaagaa atgcgttctt cacgaccatt gtgtgttagc
     6541 accacgttgc gccagtcagc ggtgtcggtc aggccacctg cggcgttgat ggcgtcgaga
     6601 atggtcagtg gtacgttggt gatcgcctgt tgaccggatt tattcacctg acctgagata
     6661 taggcctttt gtgagcggaa ggcggcgata ttaacgtcca cctgcgggtc agcgatgtac
     6721 gtcgctaagc gcccggtaat atcactgcgg atttcagcga gcgttttccc gactacgtga
     6781 accttgccga tatacgggta aaacatagtg ccgtcaggct gtacccagtt gccggtgtcg
     6841 ctggagctgc ggtactgacc agctggcgtg gtgagttccg ggtgatccca gacggtgaca
     6901 ttaagaacgt cccccggccc gacgcgatac tggtaattcg cgatctcgct ttccagcgtc
     6961 atattggggc gcgctacatt cgggcgtggg cgtaattggt caatcaggcg cggggtcagc
     7021 ggataaacat tcaccatttt gtcgagatcg aaatcagcgt cctgctgttt gatgacgtct
     7081 ttgcccatcg tcgacatatt gctgcccgga agtactgtgc aaccgcttat caaggttact
     7141 gacaccaata atggcatcaa tttcattttg gatttcatca ttgtttattt atcactttgg
     7201 cagagtaatt atcctgtgca ctattaatag caatgtcgcc atgcacattt accttgcagt
     7261 taattgaata aaaatttaac tggcatcagt cctaaaaaaa ttgatttcat ccgcaggcta
     7321 ttgacagaat aattcagact ggtctttcag gcatccagac acgctaccgc ccctggcttt
     7381 ttagctacca atacactgat tgagcttaat ttttcaaacc ctctctgcat acaatcgttg
     7441 atgagaaagg gttattgcgg aaattaactt ccgaatataa ggtgacatta tggtaattga
     7501 atattggctc gccaataatg caggaggaag tgttacagct aacggaatag caggcaa
//
LOCUS       DAZPAB010000177         7538 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZPAB010000177 DAZPAB010000000
VERSION     DAZPAB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.177"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1..1160
                     /gene="rbsA"
                     /locus_tag="ACHUPG_003329"
                     /pseudo
     CDS             1..1160
                     /gene="rbsA"
                     /locus_tag="ACHUPG_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1165..2130
                     /gene="rbsC"
                     /locus_tag="ACHUPG_003330"
     CDS             1165..2130
                     /gene="rbsC"
                     /locus_tag="ACHUPG_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR0862819.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2155..3045
                     /gene="rbsB"
                     /locus_tag="ACHUPG_003331"
                     /pseudo
     CDS             2155..3045
                     /gene="rbsB"
                     /locus_tag="ACHUPG_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3171..4100
                     /gene="rbsK"
                     /locus_tag="ACHUPG_003332"
     CDS             3171..4100
                     /gene="rbsK"
                     /locus_tag="ACHUPG_003332"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR0862820.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4104..5096
                     /gene="rbsR"
                     /locus_tag="ACHUPG_003333"
     CDS             4104..5096
                     /gene="rbsR"
                     /locus_tag="ACHUPG_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR0862821.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5062..6489)
                     /gene="mdtD"
                     /locus_tag="ACHUPG_003334"
     CDS             complement(5062..6489)
                     /gene="mdtD"
                     /locus_tag="ACHUPG_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR0862822.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6512..7204)
                     /locus_tag="ACHUPG_003335"
     CDS             complement(6512..7204)
                     /locus_tag="ACHUPG_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR0862823.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1918 a         1827 c         1986 g         1807 t
ORIGIN      
        1 aaaaccatgt atgtcgaagc ggataaattg ctggctaaac ttaacctgcg ctttaaaagc
       61 gacaagttag tgggcgatct ttccatcggt gaccagcaaa tggttgaaat cgccaaagtg
      121 ctgagctttg agtcgaaagt catcattatg gatgaaccga ccgatgcgct gaccgatacc
      181 gaaaccgaat ccctgttccg cgtcatccgc gagctgaaat cgcaaggcca ctgtattgtc
      241 tatatctccc accgcatgaa agaaatcttc gagatttgcg atgacgttac cgtttttcgt
      301 gatgggcaat ttattgctga gcgcgaagtg gcatcactga ccgaagattc gctgattgag
      361 atgatggtgg gccgcaagct ggaagatcaa tatccacacc tggacaaagc gccaggagat
      421 atccgcctga aagtcgataa tctctgcgga cctggcgtta acgatgtctc ttttacttta
      481 cgcaaaggcg aaattcttgg cgtctctggt ttgatgggcg caggtcgtac cgaactgatg
      541 aaagtgctct acggcgcact gccgcgcacc agcggttacg tcaccctgga tgggcatgaa
      601 gtcgttaccc gttcaccgca ggatggcctg gcaaacggca ttgtgtatat ctccgaagac
      661 cgtaaacgtg acggtttagt gctgggcatg tcagtaaaag agaacatgtc gctgacagcg
      721 ctgcgctact tcagccgcgc tggcggcagt ttgaagcatg ccgatgaaca gcaggctgtg
      781 agtgatttca ttcgtctgtt taatgtgaaa acgccgtcga tggaacaggc aattggtctg
      841 ctttccggtg gcaatcagca aaaagtggcg attgcccgcg gtctgatgac acccccaaag
      901 tattgatcct tgatgagcct acccgtggcg tagatgtcgg cgcgaaaaaa gagatctatc
      961 aactgattaa ccagttcaaa gccgatggct tgagtatcat tctggtgtca tcggagatgc
     1021 cagaagtatt aggcatgagc gatcgcatca tcgtcatgca tgaagggcat ctcagcgggg
     1081 aatttactcg tgagcaggcc acccaggaag tgttaatggc tgtcgctgtg ggcaagctta
     1141 atcgcgtgaa tcaggagtaa aaaaatgata acccagactg tctctggtcg ccgttatttc
     1201 acgaaagcgt ggctgatgga gcagaaatcg cttatcgctc tgctggtgct gatcgcgatt
     1261 gtctcgacgt taagcccgaa ctttttcacc atcaataact tattcaatat tctccagcaa
     1321 acttcggtga acgccattat ggcggtcggg atgacgctgg tgatcctgac gtcgggcata
     1381 gacttgtcgg taggttctct gttggcgcta accggcgcag ttgctgcatc tatcgtcggc
     1441 attgaagtca atgcgctggt ggctgtcgct gctgctctca cgttaggtgc cgcaattggt
     1501 gcggtaaccg gggtgattgt agcgaaaggt cgcgtccagg cgtttatcgc tacgctggtt
     1561 atgatgcttt tactgcgcgg cgtgaccatg gtttatacca acggtagccc agtgaatacc
     1621 ggctttactg agaacgccga tctgtttggc tggtttggta ttggtcgtcc gctgggcgta
     1681 ccgacgccag tctggatcat ggggattgtc ttcctcgcgg cctggtacat gctgcatcac
     1741 acgcgtctgg ggcgttacat ctacgcgctg ggcggcaacg aagcggcaac gcgtctttct
     1801 ggtatcaacg tcaataaaat caaaatcatc gtctattctc tttgtggtct gctggcatcg
     1861 ctggccggga tcattgaagt ggcgcgtctc tcctctgcac aacccacggc ggggactggc
     1921 tatgagctgg atgctattgc tgcggtggtt ctgggcggta cgagtctggc gggcggaaaa
     1981 ggtcgcattg ttgggacgtt gatcggcgca ttaattcttg gcttccttaa taatggattg
     2041 aatttgttag gtgtttcctc ctattaccag atgatcgtca aagcggtggt gattttgctg
     2101 gcggtgctgg tagacaacaa aaagcagtaa taacgactac aggacatctt gaatatgaac
     2161 atgaaaaaac tggctaccct ggtttccgct gttgcgctaa gcgccaccgt cagtgcgaat
     2221 gcgatggcaa aagacaccat cgcgctggtg gtctccacgc ttaacaaccc gttctttgta
     2281 tcgctgaaag atggcgcgca gaaagaggcg gataaacttg gctataacct ggtggtgctg
     2341 gactcccaga acaacccggc gaaagagctg gcgaacgtgc aggacttaac cgttcgcggc
     2401 accaaaattc tgctgattaa cccgaccgac tccgacgcag tgggtaatgc tgtgaagatg
     2461 gctaactagg cgaacattcc ggttatcact cttgaccgcc aggcaacgaa aggtgaagtg
     2521 gtgagccaca ttgcatctga taacgtactg ggcggcaaaa tcgccggtga ttacatcgcg
     2581 aagaaagcgg gtgaaggtgc caaagttatc gagctgcaag gcattgccgg tacatccgca
     2641 gcccgtgaac gtggcgaagg cttccagctg gccgttgctg ctcacaagtt taatgttctt
     2701 gccagccagc cagcagattt tgatcgcacc aaaggtttga acgtaatgca gaacctgttg
     2761 accgctcatc cggatgttca ggctgtattc gcgcagaatg atgaaatggc gctgggcgcg
     2821 ctgcgcgcac tgcaaactgc cggtaaatcg gatgtgatgg tcgtcggatt tgacggtaca
     2881 ccggatggcg aaaaagcggt gaatgatggc aaactagcag cgactatcgc tcagctaccc
     2941 gatcagattg gcgcgaaagg cgttgaaacc gcagataaag tgctgaaagg cgagaaagtt
     3001 caggctaagt atccggttga tctgaaactg gttgttaagc agtagtttta atcaggttgt
     3061 atgacctgat ggtgacataa atacgtcatc gacagatgaa cgtgtaatat aaagaaaagc
     3121 agggcacgcg ccaccctaac acggtggcgc attttatgga catcccgaat atgcaaaacg
     3181 caggcagcct cgttgttctt ggcagcatta atgctgacca cattcttaat cttcaatctt
     3241 ttcctactcc aggcgaaacc gtaaccggta accactatca ggttgcattt ggcggcaaag
     3301 gcgcgaatca ggctgtggct gctgggcgta gcggtgcgaa tatcgcgttt attgcctgta
     3361 ctggcgatga cagcattggc gagagcgttc gccagcagct cgccactgat aacatcgata
     3421 ttactccggt cagcgtgatc aaaggcgaat caacaggtgt ggcgctgatt tttgttaatg
     3481 gcgaaggtga gaatgtcatc ggtattcatg ccggcgctaa tgctgctctt tccccatcgc
     3541 tggtggaagc gcaacgtgag cgtattgcca acgcgtcagc gttattaatg cagctggaat
     3601 caccactcga aagtgtgatg gcagcggcga aaatcgccca tcaaaataag actatcgttg
     3661 cgcttaaccc ggctccggct cgcgaacttc ctgacgaact gctggcgctg gtggacatta
     3721 ttacgccaaa cgaaacggaa gcagaaaagc tcaccggtat tcgtgttgaa aatgatgaag
     3781 atgcagcgaa ggcggcgcag gtactgcatg aaaagggtat ccgtactgta ctgattactt
     3841 taggaagtcg tggtgtatgg gctagcgtga atggtgaagg tcagcgcgtt cctggattcc
     3901 gggtgcaggc tgtcgatacc attgctgccg gagatacctt taacggcgcg ttaatcacgg
     3961 cgttgctgga agaaaaacca ttgccagagg cgattcggtt tgcccatgct gccgctgcga
     4021 ttgccgtaac acgtaaaggc gcacaacctt ccgtaccgtg gcgtgaagag atcgacgcat
     4081 ttttagacag gcagaggtga cgcttggcta caatgaaaga tgttgcccgt ttggcgggcg
     4141 tttctacctc aacagtttct cacgttatca ataaagatcg cttcgtcagt gaagcgatta
     4201 ccgccaaagt tgaagcggcg attaaagaac tcaattacgc gccatcagct ctggcgcgta
     4261 gcctcaaact caatcaaaca cataccattg gcatgttgat cactgccagt accaatcctt
     4321 tctattcaga actggtgcgt ggcgttgaac gcagctgctt cgaacgcggt tatagtctcg
     4381 tcctttgcaa taccgaaggc gatgaacagc ggatgaatcg caatctggaa acgctgatgc
     4441 aaaaacgcgt tgatggctta ctgttactgt gtaccgaaac gcatcagcct tcgcgtgaaa
     4501 tcatgcaacg ttatccgaca gtgcctaccg tgatgatgga ctgggctccg ttcgatggcg
     4561 acagcgatct tattcaggat aactcgttgc tgggcggaga cttagcaacg caatatctga
     4621 tcgataaagg tcatacccgt atcgcctgta ttaccggtcc gctggataaa actccggcgc
     4681 gcctgcggtt ggaaggttat cgggcggcga tgaaacgtgc gggtctcaac attcctgatg
     4741 gctatgaagt cactggtgat tttgaattta acggcgggtt tgacgccatg cgccaactgt
     4801 tatcacatcc gctgcgtcct caggccgtct ttaccggaaa tgacgctatg gctgttggcg
     4861 tttaccaggc gttatatcag gcagagttac aggttccgca ggatatcgcg gtgattggct
     4921 atgacgatat cgaactggca agctttatga cgccaccatt aaccactatc caccaaccga
     4981 aagatgaact gggggagctg gcgattgatg tactcatcca tcggataacc cagccgaccc
     5041 ttcagcaaca acgattacaa cttactccga ttctgatgga acgcggttcg gcatagattt
     5101 acgctgtctt ttgatcaaat tattaccatc ggttgttttc agaagcatga acattgctgc
     5161 tgaagcaaca gtaataatgc ccatcgtgat aaacgtatag tggaattgtt cgacagtcgt
     5221 tgtgccttcc atcccttcat aaatgcgaag gacggccgca cttacagcaa cgcctaaact
     5281 aatcgacagt tgctgcgtga ccgccagaac actgttaccg ctgctggcgt tgtcatcggt
     5341 cagatcggca agtgtgatgg tattcatcgc ggtaaactgc gtcgacatag ccatccctaa
     5401 tataaacaac ggcaagatca gcatccatat cgccattgcc ggtgattgca aagagaactg
     5461 agcgatcatt agcccaataa tcaccgtgat ccccactaat gtatggcgat agcccagacg
     5521 acgtaagact tgggtaacca tcgattttgc aataatggaa cctaacgctg tcggtgccat
     5581 catacagccg gcaataaacg cctgataacc aaatcctacc tgtaacatca atggcataag
     5641 gaacggtaca cagccggtcc ccagacgggt tgcaatattg cctacgatac cgatcgagaa
     5701 agtgcgggtt ttaaataaat ctaatgaaat taatgggttt ggcgtacgtc gcgcatggag
     5761 aatatagaga agcagtaacc cgatgctggt gacaattacc gtcaaggcaa tccagctggc
     5821 gacaatcttt tccccgaata gctctattcc gcttgaaaag agaacaaggc tgaggccaaa
     5881 cagcaaaaag ccagtgatat cgaatctgcg tcgtgcggtg gtgaaattgg gcatatgttt
     5941 gcgcgcgtaa agaaggcccg caatacctat ggggatatta attaaaaata tccagtgcca
     6001 ggttgcccag gtgaccagca cgccgccaag aacggggcct aaaattggcc ccaccagacc
     6061 cggcatggcg acaaaattca ataccggaag aagttcatta cgaggataag cacgcagtaa
     6121 ggccggccga gcaacaggca tcatcattgc gtcgcctatc ccttgaataa cccggaagac
     6181 aaccagctgt ggtagcgaat tagaaagtgc gcaggccaga gaacccaacg tgaacagact
     6241 cacggcaagg gtaaaaatgc gacgcgtacc gaagcgatcg gctagccatc cgcttaccgg
     6301 aataagcatc gccaccgtca gcgtataact gatgatggct gattgcatcg cgagaggaga
     6361 acgattaagg ctatgagcga ttgcgggtaa ggcggtattc agaatagtgg catcaagtgc
     6421 ctgcatgaag aaggccatcg ccgcgatcca cggcaaaccc gacatactac gcttcttttt
     6481 atcgctcatt caatgtcctg ttatcgggtt atcacttatc aggtgagcgt agcagcgcct
     6541 gacaagcttt aaatgccgcg tcgccatcgc tttggataat cgcatcgaca atcgcctggt
     6601 gcagatccag ctttatcact gtgtcgctgg taattgacgt gaagtaagtg tgataaaccg
     6661 aatggaatag cgaggcgaat gaggtcaaaa acggattggc gctcatttca tagatatgct
     6721 catgccaggc catatcgact tcgatccagc gttcacggcg aaagttctct tttaatgccg
     6781 ccatttcggc cattaacgta ttgagatgcg ccttctgttc cgcggtgcca accgttgctg
     6841 ccagtaggca ggcttgcggc tccagacaga tacgcataac cagaaagtga tcgatgacct
     6901 gatgaaagtt ctcttctgtt atccaccaag taagcaattc ctgatcaaga aaattccagt
     6961 tcgattgtgg catgacccga gtaccaattc gcggtcgcgg taaaaccatc ccttttgccg
     7021 ttaacgtttt gaccgcttcg cgtaccgctg tacgactcac tccaaattgc tcgcccagct
     7081 caatctcacc gggcaaaatg gtgccgggtt catattcacc ttttaagatc cgttgcgcca
     7141 gcttctcagc cagaacatac gaaaggtttt tctgtgcagc taactgttgt gcgcttaaag
     7201 gcattactta tcttcctttt tctttttatt cctccttagt atgccaccag gaagtgtgat
     7261 tacggttgca aaaacggcaa attgcttgtt ttatggcaca ttaacggggt gttttgctga
     7321 aaaaatgcgc ggtcagaaaa ttattttaaa tttcctcttg tcaggccggg ataactccct
     7381 ataatacgcc accactgaca cggaacaacg gcaaatacgc cgccgggtca gcggggttct
     7441 cctgagaatt ccgacagaaa aagcaaaaat aaatgattga ctctgtagcg ggaaagcgta
     7501 ttatgcacac ccccccgcgc cgctgagaaa aagcgaag
//
LOCUS       DAZPAB010000178         7527 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZPAB010000178 DAZPAB010000000
VERSION     DAZPAB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.178"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(291..1691)
                     /gene="asnS"
                     /locus_tag="ACHUPG_003336"
     CDS             complement(291..1691)
                     /gene="asnS"
                     /locus_tag="ACHUPG_003336"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR0862824.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1860..3062)
                     /gene="pncB"
                     /locus_tag="ACHUPG_003337"
     CDS             complement(1860..3062)
                     /gene="pncB"
                     /locus_tag="ACHUPG_003337"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR0862825.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3328..5943
                     /gene="pepN"
                     /locus_tag="ACHUPG_003338"
     CDS             3328..5943
                     /gene="pepN"
                     /locus_tag="ACHUPG_003338"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR0862826.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(6150..6917)
                     /gene="ssuB"
                     /locus_tag="ACHUPG_003339"
     CDS             complement(6150..6917)
                     /gene="ssuB"
                     /locus_tag="ACHUPG_003339"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR0862827.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(6914..>7527)
                     /gene="ssuC"
                     /locus_tag="ACHUPG_003340"
     CDS             complement(6914..>7527)
                     /gene="ssuC"
                     /locus_tag="ACHUPG_003340"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR0862828.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         1913 a         1999 c         1908 g         1707 t
ORIGIN      
        1 taaacggaaa ttttgtttct gatggaaact ttatcgacct ggcacaaaat cctctttcag
       61 acatccagaa tgccaaaaaa tagtatgaaa attgtgctat cagctaagaa aaaactaatc
      121 cgcaataaga gtacgggtta ttttttgtgc gtaaatgttg caatgtaata ttccgtaaca
      181 ggatgatcgt tatggcgggg gtgcaaatag attgcttgac ttaacaaaca gataacttga
      241 cagaaaggat aaaaaagagc cagcaggcgc tggctctttg aggaagaaaa tcagaagctg
      301 gcgttgcgcg gagtacgtgg gaacggaatc acatcacgca cgttttgcac gccagttacg
      361 taagcaatca ggcgttcaaa accaagaccg aaacctgaat gcggaacagt accgtagcga
      421 cgcagatcgc gataccacca gtaatcttct ttattcaggc ccatttccag catacgctcg
      481 tccagaacgt ccagacgttc ttcacgctgg gagccaccaa tgatctcacc gatgcccgga
      541 gccagaacgt ccatagccgc aacggtttta ccgtcttcgt tgaggcgcat atagaacgct
      601 ttaatatctt tcggatagtt tttaaccact accggtgctt taaagtgttc ttccgccaga
      661 taacgctcat gctcagaaga gagatcgacg ccccagtaaa ccgggttttc aaacttcctg
      721 ccgcagtttt cgagaatggt cactgcgtcg gtgtaatcca cctgcgcgaa atcggcttca
      781 atgaagcgtt ccagacgtga aacggcatct ttatctacgc gttcagcgaa gaatttcatg
      841 tcgtctgcgc gttcttcgag aaccgctttg aagacatatt tcagcatggc ttcagccaga
      901 cccgcgatat cgttcaggtt agcaaacgcc acttccggct ccagcatcca gaattccgcc
      961 aggtgacggc tggtgttgga gttttcagca cggaaagtcg ggccgaaggt ataaattttg
     1021 gataatgcgc aagcgtaggt ttcgccgttc aactggccag atacggtcag gaaagactct
     1081 ttaccaaaga agtctttgtc gaaatccact ttgccctgat cgttacgcgg caggttttcc
     1141 agatccagcg tagaaacgcg gaacatttcg cctgcacctt cggtatcaga tgcggtaatc
     1201 agtggcgttg aaacccagaa gaatccctgc tcgttaaaga agcgatgcag cgcctgcgcc
     1261 agcgtatggc gaacgcgcgc gacggcacca atcaggtttg tgcgcggacg caggtgagcg
     1321 acttcacgca gatactcaat gctgtggcgt tttgccgcca tcgggtaagt gtctggatct
     1381 tcaacccaac cagcaacttc aaccttgctg gcctgaattt caaattgttg cccctggccc
     1441 ggcgatgcca cgactttacc cgtcacaatg accgagcagc cggtggtcag acgcaggacg
     1501 tcttcattgt aattgggcag agaattattg atgacagcct gtacaggatc aaagcaggaa
     1561 ccgtcataaa cggcgaggaa ggagatgcca gcttttgaat ctcggcgggt acgtacccat
     1621 ccgcgtacgg tgacttcgct gtcaacggct acgcggccct ggagtacgtc ggctacaggc
     1681 acaacgctca taatattctc tctgttaata gtcggaaaaa ataaacactt gtccacccgt
     1741 aaatgggggg gatacctatg ttacctggca tctgcaatca gacaagcaga attcgcaaat
     1801 gcagcgaaag atttcggaat taaaggtaaa aaagggagcc gattagctcc ctgatgatat
     1861 taactggctt ttttaatatg cggaaggtcg aacgctttgc gcagcgcccg aacaaacgct
     1921 ttatcatggc agatagtttt gccagggctg tcagaaagtt tcgccaccgg tttaccgtta
     1981 cactctacca acttaatcac aatattcagg ggttttacct gggggatatc gcaggtcagg
     2041 cgagtcccaa taccaaaact taattgcacg cgggaagaga agtggcggta tagctcaacc
     2101 gctttgcgta aatccagatt gtcagagaaa accagcgttt tactctgtgg atcaattccc
     2161 agcttttcat aatgtgcaat ggctttttca ccccattcaa ccgggtcgcc agagtcatga
     2221 cgcaggccct gataccgact agcgaactcg acaccgaaat cacgcaggaa agcatccata
     2281 gtgatgcagt cggttaatgc aatgccaagt tggtcgggat actcttccag ccaggcagca
     2341 agtgcagctc gctggctgtt ggctagatcc gggctgattt gctgatgtgc ctggaaccat
     2401 tcgtgtgcct gtgttcccat cggcgtgagg gaaagccgac gcgccagatc gtagttgctg
     2461 gtgcccacga accaggattc ctgttgcaga cgcttaacga tggtttcttg tacttcgcga
     2521 gaaaaacggc gacgggtgcc aaaatccatc agatggaagc gcgacatatc aagaccggcg
     2581 gttaacgccg agaagtcgac taatttgctt tccagcgtgt cgagggcttg cgcaacgtcg
     2641 gcctgcggtg agcgatagcg atgtaccatt tcactgataa ccgccagcaa aggaacttcc
     2701 cagaggatga cttcacgcca cggaccgctt aagcgaatat ccagcttgcc attatcgttg
     2761 gacacggtga cttgttccgg gttaaagcgg aactcgcgta accagttaag atagtcggcc
     2821 ttaaagaaag gcagggcaga gagccactga tattcatcat cctgcaggcg caggtgctgc
     2881 atcgcctgaa tctgttcacg aatagcatcg gcataaatac ccagcagatc gtcacctcgg
     2941 caacgaaact ccgccgcgac atgcacatcg taatagtgat gaaacacggc ttgctgcata
     3001 tgcaacttat aagcatctgt atccagcaac gagtgcagaa caggagaagc gaattgtgtc
     3061 ataggtgcgc agtagcgtcc tcgtacagga gcgtttagta caataaacat cttcaggaac
     3121 tgctggagta taccttgttt cgcaatttat tgaaccccga tcacaccata tgccaccttt
     3181 ctggtcgatg gcattacgcg gtgtatgtta taaaaatgta gcaataaagg cgtttgtacc
     3241 tgaaaagatg aagattctgc atagcgcgat ttacgcaaca ggaatagact gaacaccaga
     3301 ctctataaaa gatgctaaag gttatttatg actcaacagc cacaagccaa ataccgtcac
     3361 gattatcgtg cgccggatta ccagattact gatattgact tgacctttga cctcgacgcg
     3421 caaaagacgg tcgttaccgc ggtcagccag gctgtccgtc atggtgcatc agatgctccg
     3481 cttcgtctca acggcgaaga cctcaaactg gtttctgttc atattaatga tgagccgtgg
     3541 accgcctgga aagaagaaga agagggcgca ctggtcatca gtaatttgcc ggagcgtttt
     3601 acgcttaaga tcattaatga aataagcctg gcggcgaata cggcgctgga agggctttat
     3661 cagtcaggcg atgcgctttg cacccagtgt gaagccgaag gtttccgcca tattacgtat
     3721 tatctcgacc gcccggacgt gctggcgcgt tttaccacca aaattattgc cgataaaatc
     3781 aaatatccct tcctgctttc caatggtaac cgcgttgcgc aaggcgaact cgaaaacgga
     3841 cgccattggg tacagtggca ggacccgttc ccgaaaccgt gctacctgtt tgcgctggtg
     3901 gcaggcgact ttgatgtact gcgcgatacc tttaccacgc gttctggtcg cgaagtagca
     3961 ctggagctgt acgtcgatcg cggcaacctt gatcgcgcgc cgtgggcgat gacctcgctg
     4021 aaaaactcca tgaaatggga tgaagaacgc tttggcctgg agtatgacct cgacatctat
     4081 atgatcgtcg cggtggattt cttcaatatg ggcgcaatgg agaataaggg tctgaatatc
     4141 tttaactcca aatatgtgct ggcccgcacc gacaccgcca ccgacaaaga ttacctcgat
     4201 attgaacgcg ttatcggcca tgaatatttc cataactgga ccggtaaccg agtgacctgc
     4261 cgcgactggt tccagctcag cctgaaagaa ggtttaaccg tcttccgcga tcaggaattc
     4321 agctctgacc ttggttcccg cgcagttaac cgcatcaata atgtgcgcac catgcgcgga
     4381 ttgcagtttg cggaagacgc cagcccgatg gcgcacccaa tccgcccgga catggtcatt
     4441 gagatgaaca acttctacac cctgaccgtt tacgagaagg gcgcggaagt gatccgtatg
     4501 atccacaccc tgctgggcga agaaaacttc cagaaaggga tgcaactcta tttcgagcgt
     4561 catgatggta gcgcagcgac ctgtgacgac tttgtgcagg cgatggaaga tgcgtcgaat
     4621 gttgatctct cccatttccg ccgttggtac agccagtccg gtacgccggt tgtgaccgtc
     4681 aaagacgact acaatccgga aactgagcag tacaccctga ccatcagcca gcgcacgcca
     4741 gccacgccgg atcaggcaga aaaacagccg ctgcatattc cgtttgccat cgaattgtat
     4801 gacaacgaag gtaaagtgat cccgttgcag aaaggcggtc atccggtgaa ttccgtgctg
     4861 aacgtcaccc aggcggaaca gacctttgtt tttgataatg tctacttcca gccggtgcct
     4921 gcgctgctgt gcgaattctc tgcgccagtg aaactggaat ataagtggag cgatcagcaa
     4981 ctgaccttcc tgatgcgtca tgcgcgtaat ggtttctccc gttgggatgc ggcgcaaagt
     5041 ttgctggcaa cctacatcaa gctgaacgtc gcccgtcatc agcaagggca gccgctgtct
     5101 ctgccggtac atgtggctga cgctttccgc gcggtgctgc tcgatgagaa gattgatccg
     5161 gcgctggcgg cagaaatcct gacgctgcct tctgtcaatg aaatggctga actgttcgat
     5221 atcatagacc cgattgctat tgccgaagta cgcgaagcac tcactcgtac tctggcgact
     5281 gaactggcgg atgagctgct ggctatttac aacgcgaatt accagagcga gtaccgtgtt
     5341 gagcatgaag atattgcaaa acgcactctg cgtaatgcct gcctgcgctt ccttgctttt
     5401 ggtgaaacgc atctggctga cgtgctggtg agcaagcaat accatgaagc aaacaatatg
     5461 accgatgcgc tggcagcgct ttctgcggcg gttgctgcac agctgccttg ccgtgacgcg
     5521 ctgatgcagg agtacgacga taagtggcat caggacggtc tggtgatgga taaatggttt
     5581 atcctgcaag ccaccagccc ggcggcgaat gtgctggaga cggtgcgtgg tctgttgcag
     5641 catcgctcat ttaccatgag caacccgaac cgtattcgtt cgttgattgg tgcgtttgcg
     5701 ggcagcaacc cggcagcgtt ccatgccgaa gatggcagcg gttaccagtt cctggtggaa
     5761 atgcttaccg acctcaacag ccgtaacccg caggtagctt cacgtctgat tgaaccgctg
     5821 attcgcctga aacgttatga tgccaaacgt caggagaaaa tgcgcgcggc gctggaacag
     5881 ttgaaagggc tggaaaatct ctctggcgat ctttacgaga agataactaa agcactggct
     5941 tgatactttt gagccaaaat gcctgatgcg acgcttgacg cgtcttatca ggcctacaac
     6001 tccgttgaaa ttgctggttt tgtaggctgg ataaggcgtt cacgccgcat ccggcagccg
     6061 tgcccaactg cctgatgcga tgcttgacgt gtcttatcag gcctacaagt ccgttgaaaa
     6121 tgctggtttt gtgcgccaca tccggcatat taaccatgac gccgaatcag ttgttcagaa
     6181 tgcccacgcc gcattacccg ttgcaatact tccgcttcca gctccgctaa tcgcaccgac
     6241 cctaatcgac gcggacgggg gatatccact gtcaaatcca gactaatttt tccctcttca
     6301 attaacaaca cccggtcagc cattgctacc gcttcgctca catcatgcgt caccaacagt
     6361 acggtaaagc cgtgctgctg ccaaagtgac acaatcaaat cctgcatctc gagtcgcgtt
     6421 aaggcgtcca gtgccccgag cggttcatca agcaacaata aacccggtcg atgaatcaac
     6481 gctcttgcca gcgccacacg ctgtttctgc ccgccagaaa gtgcggcagg ccattccccg
     6541 gcgcgattct ccagtcctgc cgcagccaac gcccgacgcg cggcatcgcg ccactgacct
     6601 ttaaggccta acccaacgtt atcaatcacc gatttccacg gcagcagacg cgcatcctga
     6661 aacatcattc gcgtatcatc ctgaatttca gccagcggtg tggtgcccgc taacacatcg
     6721 cctgcggttg gcgtttccag accggccagc aggtgcagca gggtactttt accaccaccg
     6781 ctgcggccca ccaccgccac aaactgacct gccggaatat gtaaatccag ttggttcagg
     6841 acgatatttt ccgcgtaatg tttgcttact gcattgagca acaatggtgt gccctggttc
     6901 agacgagcag tattcatatc gtggcctcct tcaaatgata agccgggttc cagcgcaacc
     6961 agaggcgctc taacagctgc gcgctgacgt cagcaagttt gccgagcagg gcgtaaagaa
     7021 taatagcgac taccaccacg tctgtttgca aaaactctcg cgcattcatc gccagataac
     7081 caatgccgga attggcagaa atggtttcgg caacaatcag cgtcagccac atcaggccca
     7141 acgcaaaacg cacgccgacc ataattgagg gcagggcatc gggcaggatc acatggataa
     7201 acagcggtat gccggataat ccatagctac gcgccatctc caccagcccg cgatcgatat
     7261 tacggatgcc atgccaggtg ttgatataaa tggggaacag cgtaccgagc gccaccagaa
     7321 agattttcgc ggattcatca atgccaaacc acaaaatcac cagcgggatc agcgccagat
     7381 gcggcacgtt gcgcaacatc tgaattgagg tatccagcag cctttctccc cagcgcgaca
     7441 gtccgctaat cagtcccaga atcaggccca gcgatccgcc aattgaaaag ccaatcagcg
     7501 cccgccagga actgatcgcc agatgct
//
LOCUS       DAZPAB010000179         7402 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZPAB010000179 DAZPAB010000000
VERSION     DAZPAB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.179"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            175..1209
                     /gene="rhaT"
                     /locus_tag="ACHUPG_003341"
                     /pseudo
     CDS             175..1209
                     /gene="rhaT"
                     /locus_tag="ACHUPG_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(1206..2054)
                     /gene="rhaR"
                     /locus_tag="ACHUPG_003342"
     CDS             complement(1206..2054)
                     /gene="rhaR"
                     /locus_tag="ACHUPG_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFR0862829.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(2128..2964)
                     /gene="rhaS"
                     /locus_tag="ACHUPG_003343"
     CDS             complement(2128..2964)
                     /gene="rhaS"
                     /locus_tag="ACHUPG_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR0862830.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            3252..4721
                     /gene="rhaB"
                     /locus_tag="ACHUPG_003344"
     CDS             3252..4721
                     /gene="rhaB"
                     /locus_tag="ACHUPG_003344"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR0862831.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            4718..5977
                     /gene="rhaA"
                     /locus_tag="ACHUPG_003345"
     CDS             4718..5977
                     /gene="rhaA"
                     /locus_tag="ACHUPG_003345"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR0862832.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            6282..7043
                     /gene="rhaD"
                     /locus_tag="ACHUPG_003346"
     CDS             6282..7043
                     /gene="rhaD"
                     /locus_tag="ACHUPG_003346"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR0862833.1"
                     /translation="MKGWDERNGGNLTLRLDDADIALYHDNFHPQPRYIPLSQPMPLL
                     ANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGYHILWGLTNEAVPTSELPAHFL
                     SHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAVFTRQLWEGSTECLVVFPDGVG
                     ILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSGPTLDEAFGLIDTAEKSAQVLV
                     KVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            7053..7367
                     /gene="rhaM"
                     /locus_tag="ACHUPG_003347"
     CDS             7053..7367
                     /gene="rhaM"
                     /locus_tag="ACHUPG_003347"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR0862834.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         1773 a         1945 c         1991 g         1693 t
ORIGIN      
        1 aagggtatgg ttttgcagga aaatgcccga gatgtgaagc aaatcaccca cttaatgccg
       61 tgattgccag taaatcgaca acggcggcaa caggcgaaag gttaatcgac agcacgattt
      121 ttacactcat ctcgtcggag atgtgaagcg acgaaaaatg atgaggataa gaagatgagt
      181 aacgcgatta cgatggggat attttggcat ttgatcggcg cggccagtgc agcctgtttt
      241 tacgctccgt tcaaaaaagt aaaaaaatgg tcatgggaaa ccatgtggtc agtcggtggg
      301 attgtttcgt ggattattct gccgtgggcc atcagcgccc tgttactacc ggatttctgg
      361 gcgtattaca gctcgtttag tctctctacg ctgctgcctg tttttctgtt cggcgctatg
      421 tgggggatcg gtaatatcaa ctatggcctg accatgcgtt atctcggcat gtcgatggga
      481 attggcatcg ccattggcat tacgttgatt gtcggtacgc tgatgacgcc aattatcaac
      541 ggcaatttcg atgtgctgat taacaccgaa ggcggacgca tgacgttact cggcgttctg
      601 gtggcgctga ttggcgtagg gattgtaact cgagccgggc agttgaaaga gcgcaagatg
      661 ggcattaaag ccgaagagtt caatctgaaa aaagggctgg tgctggcggt gatgtgcggc
      721 attttctctg ccgggatgtc ctttgcgatg aacgccgcaa aaccgatgca tgaagccgct
      781 gccgcacttg gcgtcgatcc actgtatgtt gctctgccaa gctatgttgt catcatgggc
      841 ggcggcgcga tcattaacct cggtttctgt tttattcgtc tggcaaaagt gaaggatttg
      901 tcgctaaaag ccgacttctc gctggcaaaa ccgctgatca ttcacaatgt gttactctcg
      961 gcactgggcg ggttgatgtg gtatctgcaa ttctttttct atgcctaggg ccacgcccgc
     1021 attccggcgc agtatgacta catcagttgg atgctgcata tgagtttcta tgtattgtgc
     1081 ggcggtatcg tcgggctggt gctgaaagag tggaacaata caggacgccg tccggtaacg
     1141 gtgttgagcc tcggttgtgt ggtgattatt gtcgccgcta acatcgtcgg catcggcatg
     1201 gcgaattaat ctttctgcga attgagatga cgccactggc tgggcgtcat cccggtttcc
     1261 cgggtaaaca ccaccgaaaa atagttacta tcttcaaagc cacattcggt cgaaatatca
     1321 ctgattaaca ggcggctatg ctggagaaga tattgcgcat gacacactct gacctgccgc
     1381 agatattgat tgatggtcat tccagtctgc tggcgaaatt gctgacgcaa aacgcgctca
     1441 ctgcacgatg cctcatcaca aaatttatcc agcgcaaagg gacttttcag gctagccgcc
     1501 agccgggtaa tcagcttatc cagcaacgtt tcgctggatg ttggcggcaa cgaatcactg
     1561 gtgtaacgat ggcgattcag caacatcacc aactgcccga acagcaactc agccatttcg
     1621 ttagcaaacg gcacatgctg actactttca tgctcaagct gaccgataac ctgccgcgcc
     1681 tgcgccatcc ccacgctacc taagcgccag tgtggttgcc ctgcgctggc gctaaatccc
     1741 ggaatcgctc cctgccagtc aagattcagc ttcagccgct ccgggcaata aataatattc
     1801 tgcaaaacca gatcgttaac ggaagcgtag gagtgtttat cgtcagcatg aatataaaag
     1861 agatcgccac gggtaatgcg ataaggacga tcgttgagta catgcagacc attaccgcgc
     1921 cagacaatca ccagctcaca aaaatcatgt gtatgttcgg caaagacatc ttgcggataa
     1981 cggtcagcca cagcgactgc ctgctggtcg ctggcaaaaa aatcatcttt gagaagtttt
     2041 aactgatgcg ccaccgtggc tacctcggcc agagaacgaa gttgattatt cgcaatatgg
     2101 cgtacaaata cgttgagaag attcgcgtta ttgcagaaag ccatcccgtc cctggcgaat
     2161 atcacgcggt gaccagttaa actctcggcg aaaaagcgtc gaaaagtggt tactgtcgct
     2221 gaatccacag cgataggcga tgtcagtgac gctggcctcg ctgtggcgta gtagatgtcg
     2281 ggctttcatc agtcgcaggc ggttcaggta tcgctgaggc gtcagtcccg tttgctgctt
     2341 aagctgccga tgtagcgtac gcagtgaaag agaaaattga tccgccacgg catcccaatt
     2401 cacctcatcg gcaaaatggt cctccagcca ggccagaagc aagttgagac gtgatgcgct
     2461 gttttccagg ttctcctgca aactgctttt acgcagcaag agcagtaatt gcataaacaa
     2521 gatctcgcga ctggcggtcg agggtaaatc attttcccct tcctgctgtt ccatctgtgc
     2581 aaccagctgt cgcacctgct gcaatacgct gtggttaacg cgccagtgag acggatactg
     2641 cccatccagc tcttgtggca gcaactgatt cagcccggcg agaaactgaa atcgatccgg
     2701 cgagcgatac agcacattgg tcagacacag attatcggta tgttcataca gatgccgatc
     2761 atgatcgcgt acgaaacaga ccgtgccacc ggtgatggta tagggctgcc cattaaacac
     2821 atgaataccc gtgccatgtt cgacaatcac aatttcatga aaatcatgat gatgttcagg
     2881 aaaatccgcc tgcgggagcc ggggttctat cgccacggac gcgttacaag acggaaaaaa
     2941 atccacacta tgtaatacgg tcatactggc ctcctgatgt cgtcaacacg gcgaaatagt
     3001 aatcacgagg tcaggttctt accttaaatt ttcgacggaa aaccacgtaa aaaacgtcga
     3061 tttttcaaga tacagcgtga attttcagga aatgcggtga gcatcacatc accacaattc
     3121 agcaaattgt gaacatcatc acgttcatct ttccctggtt gccaatggcc cattttcctg
     3181 tcagtaacga gaaggtcgcg aattcaggcg ctttttagac tggtcgtaat gaaattcagc
     3241 aggatcacat tatgaccttt cgcaattgtg tcgccgtcga tctcggcgca tccagtgggc
     3301 gcgtgatgct ggcgcgttac gagcgtgaat gccgcagcct gacgctgcgc gaaatccatc
     3361 gttttaacaa tgggctgcat agccaaaacg gttatgtcac ctgggatgtg gatagcctgg
     3421 aaagtgccat tcgccttgga ttaaacaagg tgtgcgagga agggattcgt atcgatagca
     3481 ttgggattga tacctggggc gtggactttg tgctgctcga ccaacagggt cagcgtgtgg
     3541 gcctgcccgt tgcttatcgc gatagccgca ccaatggcct gatgccgcag gcacaacagc
     3601 aactcggcaa acgcgatatt tatcagcgta gcggcatcca gtttctaccc ttcaatacgc
     3661 tttatcagtt gcgtgcgctg acggagcaac aacctgaact tattccacac attgctcacg
     3721 ctctgctgat gccggattac ttcagttatc gcctgaccgg caagatgaac tgggaatata
     3781 ccaatgccac caccacacaa ctggtcaata tcaatagcga cgactgggac gagtcgctac
     3841 tggcgtggag cggggccaac aaagcctggt ttggtcgccc gacgcatccg ggtaatgtca
     3901 ttgggcactg gatttgcccg cagggtaatg agattccggt ggtcgccgtt gccagccatg
     3961 ataccgccag cgcggttatc gcctcgccgt taaacggttc acgcgccgct tatctctctt
     4021 ctggcacctg gtcattgatg ggcttcgaaa gccagacgcc atttaccaat gacacggcgc
     4081 tggcagccaa catcaccaat gaaggcgggg cggaaggtcg ctatcgggtg ctgaaaaata
     4141 ttatgggctt atggctgctt cagcgagtgc ttcaggaacg gcaaatcaac gatctcccgg
     4201 cgcttatcgc cgcgacacag gcacttccgg cctgccgctt catcatcaat cccaatgacg
     4261 atcgctttat taatcctgac gagatgtgca gcgaaattca ggctgcgtgt cgggaaacgg
     4321 cgcaaccgat cccgggaagt gatgctgaac tggcgcgctg tattttcgac agtctggcgc
     4381 tgctgtatgc cgatgtgttg catgagctgg cgcagctgcg cggtgaagat ttctcgcaac
     4441 tgcatattgt cggcggcggc tgccagaaca cgctgctcaa ccagctatgc gccgatgcct
     4501 gcggtattcg ggtgatcgcc gggcctgttg aagcctcaac gctcggcaat atcggcatcg
     4561 agttaatgac gctggatgaa ctcaacaatg tggatgattt ccgtcaggtc gtcagcacca
     4621 ccgcgaatct gaccaccttt acccctaatc ctgacagtga aattgcccac tatgtggcgc
     4681 agattcactc tacacgacag acaaaggagc tttgcgcatg accactcaac tggaacaggc
     4741 ctgggagctg gcgaaacagc gtttcgcggc ggtggggatt gatgtcgagg aggcgctgcg
     4801 ccaacttgat cgtttacccg tttcaatgca ctgctggcag ggcgatgatg tttccggttt
     4861 tgaaaacccg gaaggttcgc tgaccggggg gattcaggcc acaggcaatt atccgggcaa
     4921 agcgcgtaat gccagtgagc tacgtgccga tctggaacag gctatgcggc tgattccggg
     4981 gccgaaacgg cttaatttac atgccatcta tctggaatca gatacgccag tctcgcgcga
     5041 ccagatcaaa ccagagcact tcaaaaactg ggttgaatgg gcgaaagcca atcagctcgg
     5101 tctggatttt aacccctcct gcttttcgca tccgctaagc gccgatggct ttacgctctc
     5161 gcatcccgac gacagcattc gccagttctg gattgatcac tgcaaagcca gccgtcgcgt
     5221 ttcggcctat tttggcgagc aactcggcac accatcggtg atgaacatct ggatcccgga
     5281 tggtatgaaa gatatcaccg ttgaccgtct cgccccgcgt cagcgtctgc tggcagcact
     5341 ggatgaggtg atcagcgaga agctaaaccc tgcgcaccat atcgacgccg ttgagagcaa
     5401 attgtttggc attggcgcag agagctacac ggttggctcc aatgagtttt acatggggta
     5461 tgccaccagc cgccagactg cgctgtgcct ggacgccggg cacttccacc cgactgaagt
     5521 gatttccgac aagatttccg ccgccatgct gtatgtaccg cagttgctgc tgcacgtcag
     5581 ccgtccggtt cgctgggaca gcgatcacgt agtgctgctg gatgatgaaa cccaggcaat
     5641 tgccagtgag attgtgcgtc acgatctgtt tgaccgggtg catatcggcc ttgacttctt
     5701 cgatgcctct atcaaccgca ttgccgcgtg ggtcattggt acacgcaata tgaaaaaagc
     5761 cctgctgcgt gcgttgctgg aacctaccgc tgagctgcgc aagctggaag cggcgggcga
     5821 ttacactgcg cgtctggcac tgctggaaga gcagaaatcg ttgccgcggc aggcggtctg
     5881 ggaaatgtat tgccaacatc acgatacgcc agcaggtagc gaatggctgg agagcgtgcg
     5941 ggcttatgag aaagcgattt tgagccagcg tgggtaaaca ctgccggatg cggcacgagc
     6001 gccttatccg gcctacgggt cgaatgtcca tgcgggctgt caccctcacc ccggccctct
     6061 ccctgaaagg gcgagggaga aaaacatagc ctcgtgcggc aacggagctg aacaccgctc
     6121 cggacagtcc cctcgcccct acggggagag ggttagggtg aggggaacaa gccgcaccat
     6181 gtctaaacaa ggcaacctaa tttacaggaa cacagaacat gcaaaacatt acccagtcct
     6241 ggtttgtcca gggaatgatc aaagccacca ccgatgccta gctgaaaggc tgggacgagc
     6301 gcaacggcgg caacctgacg ctgcgcctgg atgacgccga tatcgcgctt tatcacgaca
     6361 acttccaccc acaaccgcgc tatattccgc tcagccaacc catgccttta ctggcaaata
     6421 cgccgtttat tgtcaccggc tccggcaaat tcttccgtaa cgtccagctc gatcctgcag
     6481 ctaacttagg cgtcgtaaaa gtcgacagcg atggcgcggg ctaccatatt ctctgggggt
     6541 taaccaacga agccgttcca acttctgaac ttccggctca ctttctctcc cactgcgagc
     6601 gcattaaagc caccaacggt aaagatcggg tgatcatgca ctgccacgcc accaacctga
     6661 tcgccctcac ctatgtactg gaaaacgaca ccgcggtctt tactcgccaa ctgtgggaag
     6721 gcagcaccga atgtctggta gtattcccgg atggcgttgg cattctgccg tggatggtgc
     6781 ccggcacgga cgaaatcggc caggcgaccg cacaagagat gcaaaaacat tcgctggtgt
     6841 tgtggccctt ccacggtgtg ttcggttccg gaccgacgct ggatgaagcc tttggtttaa
     6901 tcgacaccgc agaaaaatca gcacaagtat tagtgaaggt ttattcgatg ggcggcatga
     6961 aacagaccat cagccgtgaa gagttgatag cgctcggcaa gcgcttcggc gttacaccac
     7021 tagccagtgc gctggcgctg taaggagcaa acatgatccg caaagccttt gtcatgcagg
     7081 taaaccccga cgcccacgaa gagtatcagc gtcggcataa tcccatctgg ccggaactgg
     7141 aagcagtgtt gaaatctcac ggtgtgcata actacgccat ttacctcgac aaagcgcgta
     7201 atctgctgtt tgccatggta gagattgaat ctgaagaacg gtggaatgcg gtagccagta
     7261 ctgatgtttg ccaacgttgg tggaaatata tgaccgatgt tatgcccgct aacccagata
     7321 acagcccggt gagtagcgag ctgcaagaag tgttttacct gccgtaattg cccaatgtgc
     7381 cggagatgcc tccggcacaa ta
//
LOCUS       DAZPAB010000180         7370 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZPAB010000180 DAZPAB010000000
VERSION     DAZPAB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.180"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            115..1512
                     /gene="ulaA"
                     /locus_tag="ACHUPG_003348"
     CDS             115..1512
                     /gene="ulaA"
                     /locus_tag="ACHUPG_003348"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFR0862835.1"
                     /translation="MEILYNIFTVFFNQVMTNALLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            1528..1833
                     /gene="ulaB"
                     /locus_tag="ACHUPG_003349"
     CDS             1528..1833
                     /gene="ulaB"
                     /locus_tag="ACHUPG_003349"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR0862836.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            1843..2307
                     /gene="ulaC"
                     /locus_tag="ACHUPG_003350"
     CDS             1843..2307
                     /gene="ulaC"
                     /locus_tag="ACHUPG_003350"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR0862837.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2321..2971
                     /gene="ulaD"
                     /locus_tag="ACHUPG_003351"
     CDS             2321..2971
                     /gene="ulaD"
                     /locus_tag="ACHUPG_003351"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFR0862838.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2981..3835
                     /gene="ulaE"
                     /locus_tag="ACHUPG_003352"
     CDS             2981..3835
                     /gene="ulaE"
                     /locus_tag="ACHUPG_003352"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR0862839.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            3835..4521
                     /gene="ulaF"
                     /locus_tag="ACHUPG_003353"
     CDS             3835..4521
                     /gene="ulaF"
                     /locus_tag="ACHUPG_003353"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR0862840.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4650..4925)
                     /gene="yjfY"
                     /locus_tag="ACHUPG_003354"
     CDS             complement(4650..4925)
                     /gene="yjfY"
                     /locus_tag="ACHUPG_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFR0862841.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5251..5646
                     /gene="rpsF"
                     /locus_tag="ACHUPG_003355"
     CDS             5251..5646
                     /gene="rpsF"
                     /locus_tag="ACHUPG_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR0862842.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5653..5967
                     /gene="priB"
                     /locus_tag="ACHUPG_003356"
     CDS             5653..5967
                     /gene="priB"
                     /locus_tag="ACHUPG_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR0862843.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            5972..6199
                     /gene="rpsR"
                     /locus_tag="ACHUPG_003357"
     CDS             5972..6199
                     /gene="rpsR"
                     /locus_tag="ACHUPG_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR0862844.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6241..6690
                     /gene="rplI"
                     /locus_tag="ACHUPG_003358"
     CDS             6241..6690
                     /gene="rplI"
                     /locus_tag="ACHUPG_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR0862845.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(6761..7324)
                     /locus_tag="ACHUPG_003359"
                     /pseudo
     CDS             complement(6761..7324)
                     /locus_tag="ACHUPG_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         1756 a         1799 c         2014 g         1801 t
ORIGIN      
        1 cgcgtcacat ttaatcataa ataatcttgt tgtgattact tttgaaaatt agagtgagtg
       61 cacaacattc cgggtgtgcg gaatacccgg ttacctcttc ttcaggagat cgttatggag
      121 atcctctaca acatctttac cgtgtttttt aaccaggtca tgaccaatgc cctgttgttg
      181 ctgggtattg tgacctgtct gggctacatc ctactgcgca aaagtgtcag cgttattatt
      241 aaaggcacga ttaaaaccat aattggtttc atgttgttgc aggcagggtc cggcatcctc
      301 accagcacct tcaaaccggt ggtggcgaaa atgtccgaag tctacggcat taacggcgca
      361 atttccgata cttacgcgtc gatgatggca accatcgacc gcatgggcga tgcctatagc
      421 tgggtaggtt acgcggtgtt gttagcgctg gcgctgaaca tctgttacgt gctgctgcgt
      481 cgcattaccg gtattcgtac catcatgctg actggtcata tcatgttcca gcaggcgggg
      541 ctgattgccg ttacgttgtt tattttcggc tactccatgt ggacaaccat tatctgcacg
      601 gcgattctgg tttcgctcta ctggggcatt acctccaaca tgatgtacaa gccgactcag
      661 gaagtgacgg acggctgcgg tttctccatc ggtcaccagc aacagtttgc atcattaatt
      721 gcctataaag ttgcgccgtt cctcggcaaa aaagaggaaa gcgttgaaga cctcaaactg
      781 ccaggctggc tgaatatttt ccacgacaac atcgtctcca cggcgattgt catgaccatc
      841 ttctttggtg ccattctgct ctccttcggt atcgacaccg tgcaggcgat ggcaggcaaa
      901 gtgcactgga cggtctatat cctgcaaacc ggtttttcct tcgcggtggc gatcttcatc
      961 atcactcagg gtgtgcgcat gtttgtggcg gaactctctg aagcgtttaa cggtatctcc
     1021 cagcgcctga tccctggtgc ggttctggcg attgactgtg cggctattta tagcttcgct
     1081 ccgaacgccg tggtctgggg ctttatgtgg ggcaccatcg gtcagctgat tgcggttggc
     1141 atcctggtcg cctgcggttc ctcgatcctg attatcccag gctttatccc gatgttcttc
     1201 tccaacgcca ccatcggcgt gttcgctaac cacttcggcg gctggcgtgc ggcgctgaag
     1261 atttgtctgg tgatggggat gatcgaaatc tttggttgcg tctgggtggt gaaacttacc
     1321 ggtatgagtg cctggatggg gatggcggac tggtcgattc tggcaccgcc gatgatgcag
     1381 ggcttcttct ccatcggtat cgcctttatg gccgtcatca ttgtaattgc actggcttat
     1441 atgttcttcg ctggccgcgc actgcgcgca gaagaagatg cagaaaaaca attggcagaa
     1501 cagtctgctt aataaggagt tttgattatg accgtacgta ttctggctgt gtgtggcaac
     1561 ggacaaggca gttccatgat catgaagatg aaagtggacc agtttttaac ccaatcaaac
     1621 attgaccaca cggtaaatag ctgcgcggtt ggcgaataca aaagcgagtt gagcggcgcg
     1681 gatatcatca tcgcttctac gcacattgcg ggcgaaatca ccgtgaccgg caacaaatac
     1741 gtggttggcg tgcgcaacat gctctctcct gccgactttg gcccgaaact gctggaagtg
     1801 atcaaagcgc atttcccgca ggatgtgaag taaggacgcg ccatgaaatt acatgattcg
     1861 ctggcggaga ataaatccat ccgcctgcag gctgaagctg agacatggca ggacgcagta
     1921 aaaatcggtg ttgacctgct ggtggcggcg gatgttgtcg agccgcgtta ctaccaggcg
     1981 attctggatg cggttgaaca gcacggtccg tactttgtgc tcgcaccagg cctggcaatg
     2041 ccgcacggtc gcccggaaga gggcgttaag aaaaccggtt ttgcgctggt gacgctgaaa
     2101 aagccgctgg agttcaacca cgaagataac gacccggtgg atatcctcat caccatggcg
     2161 gcggtcgatg ccaacacgca ccaggaagtg ggcatcatgc agatcgtcaa cctgtttgaa
     2221 gatgaagaaa atttcgaccg tttacgtgcc tgccgtaccg agcaggaagt actggatctc
     2281 attgaccgca ccaacgcggc agcttaagaa ggaattgaag atgtcattac cgatgttgca
     2341 agtcgcgctg gacaaccaga ctatggatag cgcctacgaa accactcgcc tgattgccga
     2401 agaagtcgac attatcgaag tgggtaccat tctgtgcgtg ggcgaaggcg tgcgtgcggt
     2461 tcgcgatctg aaagcgctct acccgcacaa aatcgtgctg gcagacgcca aaattgccga
     2521 tgcgggcaaa attctttctc gtatgtgctt cgaagccaac gctgactggg tgaccgtgat
     2581 ttgttgtgcg gatatcaaca ccgccaaagg tgcgctggac gtagcaaaag agtttaacgg
     2641 cgacgtgcag atcgaactga ccggttactg gacctgggaa caggcgcaac agtggcgcga
     2701 cgcaggtatt cagcaggtgg tttatcaccg cagccgtgac gcgcaggccg ctggcgtggc
     2761 gtggggtgaa gccgatatca ctgcgatcaa acgtctttcc gatatgggct tcaaagtcac
     2821 cgtcaccggt ggcctggcgc tggaagatct gccgctgttc aaaggcattc caattcatgt
     2881 gtttatcgcc ggtcgcagca tccgtgatgc cgcttctccg gttgaagccg cgcgtcagtt
     2941 caaacgttcc atcgccgaac tgtggggcta aggagcggat atgttgtcca aacaaatccc
     3001 gcttggcatc tatgaaaaag cgctccccgc cggggagtgc tggctggaac gcctgcgact
     3061 ggcaaaaacg ttgggcttcg attttgtcga gatgtcggta gatgaaacta acgagcgcct
     3121 gtcgcgcctt gactggagcc gcgaacagcg tctggcgctg gtgaatgcta ttgttgaaac
     3181 cggcgtgcgt gtgccgtcca tgtgcctttc tgcccatcgt cgtttcccgt tgggcagcga
     3241 agatgacgcg gtgcgggcgc aggggctgga gattatgcgt aaagccatcc agttcgccca
     3301 ggacgttggc attcgcgtga tccagctggc gggctatgac gtttactatc aggaagccaa
     3361 taacgaaacg cgtcgtcgtt tccgtgacgg cctgaaagag agcgttgaga tggcgagccg
     3421 cgcgcaggtg acgctggcga tggagatcat ggattatccg ttgatgaact ccatcagcaa
     3481 ggcgctgggt tacgcgcact atctcaacaa cccgtggttc cagctctacc cggatatcgg
     3541 caacctgtcg gcgtgggaca acgatgtgca gatggagttg caggccggaa tcgggcatat
     3601 cgtcgcggtg catgtgaaag acaccaaacc gggcgtcttc aaaaacgtgc catttggcga
     3661 aggtgtggtg gatttcgaac gttgcttcga aacgctcaaa cagagtggct attgcgggcc
     3721 gtacctgatt gagatgtgga gcgaaacggc ggaagacccg gcggcagaag tggtgaaagc
     3781 gtgtgattgg gtgaaagcgc gcatggccaa agcaggcatg gtggaggcgg cataatgcaa
     3841 aagctaaaac agcaggtgtt tgaagccaac atggatctgc cgcgctatgg gctggtgacc
     3901 tttacttggg gcaacgtcag cgctatcgac cgcgaacgcg ggctggtggt gatcaagccc
     3961 agcggcgttg cctacgaaac catgaaagca gacgatatgg tggtggtcga tatgagcggc
     4021 aaggtggtgg aaggggagta tcgcccatct tccgacactg cgacgcatct cgaactctac
     4081 cgtcgttacc cgtcgcttgg cggcattgtc catacccact ccactcatgc caccgcatgg
     4141 gcgcaggcgg ggctggcgat cccggcatta ggcaccacgc acgctgacta cttctttggt
     4201 gatatcccgt gtacgcgtgg attaagcgaa gaagaggtgc agggtgagta tgaactgaac
     4261 actggcaaag tgattatcga aacgctgggc aacgccgagc cgctgcatac gccgggaatt
     4321 gtggtgtatc agcacggacc gttcgcctgg gggaaagatg cccacgacgc ggtgcataac
     4381 gcggtagtga tggaagaagt ggcgaaaatg gcgtggattg cgcgcagtat taatccacaa
     4441 ctcaatcata tcgacagcta cctgatgaat aaacacttca tgcgtaagca cggtcctaac
     4501 gcttattacg ggcagaagta aaacacgcgc tgcggaaatt tccttcctcg ggagataact
     4561 ggtctaattc cgcagccgtt tttcaaaaaa agccccctgc gaagggggca aagcaaacta
     4621 tggcaatgtt tcgttggtta tacctggtgc tagcgataaa tatccgcgct ggcgtgcata
     4681 ttgccgttac tccccggttc ccgcatcaga attacgtggt agtacgttgc gccttgcgca
     4741 tctgtttctt catttaatgc ctgacgtgct tcgctttcag tggcgaaatt atgattgata
     4801 taaatcacgc ctaagctttg cacatcgtcc atatttctgg cctggtggtt attaatttca
     4861 atggctgccc atgtatttgc acttagcaaa agcacagcca gaagggctaa aactcgactg
     4921 aacatagata cctcctcgac ggctgacttt gtgtgctctc ctctgtgatg atcttctgat
     4981 ttaattttaa tcaatgataa agaagttgat ggtgaccatt tctgatgcag ttgttcaaaa
     5041 aacactgtga cggaatgtga tgaacttcaa atcagcggtt tagaggttat ttgcgaaagg
     5101 ggagatttat ttctgctccg cccttgagtt tagcggggta tgccagtact ataacgcgtc
     5161 atttttcagc cgacctttaa cacgttcctt gcctccccgg gattcggctg acccagacag
     5221 gaggctgaat aatccgtaag gagcaattcg atgcgtcatt acgaaatcgt ttttatggtc
     5281 catcctgacc agagcgaaca ggttccgggc atgatcgagc gctacactgc tgccatcact
     5341 ggtgcagaag gcaagatcca ccgtctggaa gactggggcc gccgtcagct ggcttacccg
     5401 atcaacaaac tgcacaaagc acactacgtt ctgatgaacg ttgaagctcc gcaggaagtg
     5461 atcgatgagc tggaaactac cttccgcttc aacgatgccg ttatccgcag catggttatg
     5521 cgtaccaagc acgctgttac cgaagcatct ccgatggtta aagcgaaaga cgagcgccgt
     5581 gagcgtcgcg atgatttcgc aaacgaaacc gctgatgatg ctgaagctgg ggattctgaa
     5641 gagtaatttc tgatgaccaa ccgtctggtg ttgtccggca ccgtgtgcag gactcccctt
     5701 cgaaaggtca gtccatcagg aattcctcac tgccagttcg tgcttgagca tcgttctgtg
     5761 caggaggaag ccggctttca ccggcaggca tggtgtcaaa tgcccgttat tgttagcgga
     5821 cacgaaaacc aggccattac tcacagtata acggtcggca gtcgcataac cgttcagggg
     5881 ttcatttcat gccacaaggc aaagaacgga ctgagcaaaa tggttttgca tgccgagcag
     5941 attgaattga tagattctgg agactagcca tatggcacgt tatttccgtc gtcgcaagtt
     6001 ctgccgtttc accgcggaag gcgttcaaga gatcgactat aaagatatcg ctacgctgaa
     6061 aaactacatc accgaaagcg gtaagattgt cccaagccgt atcaccggta cccgtgcaaa
     6121 ataccagcgt cagctggctc gcgctatcaa acgcgctcgc tacctgtccc tgctgccgta
     6181 cactgatcgc catcagtaat cggtcacggt ccattaatac gactttgaga ggataaggta
     6241 atgcaagtta ttctgcttga taaagtagca aacctgggta gcctgggtga tcaggtaaac
     6301 gttaaagcgg gctatgctcg taacttcctg gtaccgcagg gtaaagctgt tccagctacc
     6361 aagaaaaaca ttgaattctt cgaagcacgt cgcgctgaac tggaagctaa actggctgaa
     6421 gttctggcag ctgctaatgc tcgcgctgag aaaatcaatg cactggaaac tgttaccatc
     6481 gcgtctaaag ctggcgacga aggtaaactg ttcggttcca tcggtactcg cgacatcgct
     6541 gacgctgtaa ctgcagctgg cgttgaagtg gctaagagcg aagttcgtct gccgaacggc
     6601 gttctgcgta ccactggcga acacgaagtg agcttccagg ttcacagcga agtattcgcg
     6661 aaagtgatcg taaacgtagt agctgaataa ttcgttattc aacgagacgt aaaaacgccg
     6721 accattggtc ggcgttttgc tttctatttt tcgtcaggca ttaatttcgc aagtagatcc
     6781 attcaccatt ctcttgtgtc cataagtcag gatggtttgc tggcgttaga tgcatacgat
     6841 cactccttat gtctcggtcc tgcggcactc gctcgaaacc taaacccgag tagattgcgg
     6901 ctgaatttga taaggaacgt agaccaattt tcaaatcatg gggtaaatca gaatacgcgt
     6961 ttagcaatgc agcaatgatg aatccgaggg cattagaaac gcctgtaaca gtgtgaagtt
     7021 ggtcgatata aagatcgatt gttggtagtt cagatgagca gcaattaggc atttgggcag
     7081 gaagggttac aacgtatcct atcgtttggc cggtatagtc tggagtacta taaccaggtc
     7141 ccaaaacaac ctcgtgtatt gtgtatttac tgttgggttc gaaggattcg acatcttgca
     7201 ataaatattt cagttctgcg gttaacctcg cacccgcgta agctttattg tatttctggg
     7261 cgtgaatatt atctaatgta gcttcatggt tccatccaaa agaattatca ccattcattg
     7321 ttactgaatc gccacgggtt taacagacac ctcagagtca tttaagatgg
//
LOCUS       DAZPAB010000181         7363 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZPAB010000181 DAZPAB010000000
VERSION     DAZPAB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.181"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACHUPG_003360"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="ACHUPG_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFR0862846.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="ACHUPG_003361"
     CDS             complement(1433..1759)
                     /locus_tag="ACHUPG_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFR0862847.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="ACHUPG_003362"
     CDS             1894..2235
                     /locus_tag="ACHUPG_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFR0862848.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="ACHUPG_003363"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="ACHUPG_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="ACHUPG_003364"
     CDS             complement(2833..3543)
                     /locus_tag="ACHUPG_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFR0862849.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="ACHUPG_003365"
     CDS             3651..3956
                     /locus_tag="ACHUPG_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFR0862850.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..5568
                     /locus_tag="ACHUPG_003366"
                     /pseudo
     CDS             4155..5568
                     /locus_tag="ACHUPG_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(6298..7287)
                     /locus_tag="ACHUPG_003367"
     CDS             complement(6298..7287)
                     /locus_tag="ACHUPG_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862851.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
BASE COUNT         1945 a         1873 c         1762 g         1783 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagtgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgacaggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc
     4441 tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca
     4501 atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc
     4561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta
     4621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     4681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     4741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     4801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     4861 cgcatgagcg gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     4921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg
     4981 ctccctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     5041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     5101 accttacccg cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt
     5161 gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac
     5221 cgtatcatca aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc
     5281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct
     5341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat
     5401 accattacga tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc
     5461 ttcagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaactacaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc aggaccctcc ggtggcctcg
     5701 gtctggactt gaggcgggct atgggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagta caaacctaag cccccagaag
     5821 cccacccaca gccttagaca gagtgtcgaa tacattcaca ccgagtcgac cggagaggac
     5881 catatagagg cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag
     5941 gtcttcggaa gtcatggaca taaagccccc cctatcttct attgcactac cgcactagac
     6001 aactagtaca aaacaaaggc ccagaccatt ggtccagacc gtagttagtt aatcacagtg
     6061 acaagccacc acctgaagtc ctgctgatat tcagtccaga cttacctctg ctcatggtct
     6121 tacctccctc ggtctcctga ccttgggtat cgactacagc ttgctgagcg tcatctggag
     6181 gagctacagg aggagctaca ggaggcggga ccgaagcccc accttgagtc ccaccaccac
     6241 ctgtcaggcc cttggcgatg gacctgaagc ctttactaat gctcttaccc attggtctta
     6301 cgcttcggtc ttggtgaact tcaggcgttt gactacaggc aggacggcag tagctgagcc
     6361 agtgcccatc ttgatggtcg tagcagcttc agcgacaacg aacactttac cgaggatttc
     6421 caagtcgtgg gactgagagt cgccatcacg tgaaaggacg accgtaatat tctcaccgtc
     6481 gtgacggaag cagccgaagc tcagggccgt gaagcgttta gccagcatga atacaccctt
     6541 gtcttcaccg ttgtaatagg cgatgtctgt ccccagcata tcgacgatag cgtcttcacc
     6601 ggagaagcct tgcttagcag ccagacggga cagtgctttc agttggctct catgagcgat
     6661 aataccgtag tcagacacct cagaaccgaa agaagactct gtgttctcat acagagcgtc
     6721 catgatagcg tctgccttag cggtcaggct accagtcagg acaggaagct ctacggtctt
     6781 cagcagctta gaggattcca gaacgtcgac catgcgagcg ttgatggtac ggaccacatt
     6841 ctcacagatg ttcatagcgt cctgctgaag tgtgccctga gtgaatccgg tggatgactt
     6901 ggcgtcccag agcttggtct gctcagagat gagaatagcc tgctcgattg gttcgaccac
     6961 gatttcttgt ccagacggaa cggtcacgac ttcaggtcgc ttgccaccac ggacaggaac
     7021 gaccacagag cggtcagcca gagtgtgagc aatgattggg gtcggtaagc tgttacgggt
     7081 cggcaggact tctacaggga acgggatgtt ctcttgagcg tctttaagta caccgttcag
     7141 catagcctta accgtcttag ctaactggag ggcatagacc tgcgggtcag cgttagtcgg
     7201 cagaccttca ttgacctgaa tgttgtcggt atcggtgatg cgtactggct caatgccaaa
     7261 ctggaaagcg tctttaatcg tagtcataat aatcaatctc ctaaattagt ttaaatactg
     7321 tgagtcctca aaactgagga gtccctaaca tcgggtcagg gac
//
LOCUS       DAZPAB010000182         7310 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZPAB010000182 DAZPAB010000000
VERSION     DAZPAB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.182"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..478)
                     /gene="folK"
                     /locus_tag="ACHUPG_003368"
     CDS             complement(<1..478)
                     /gene="folK"
                     /locus_tag="ACHUPG_003368"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR0862852.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(475..1872)
                     /gene="pcnB"
                     /locus_tag="ACHUPG_003369"
     CDS             complement(475..1872)
                     /gene="pcnB"
                     /locus_tag="ACHUPG_003369"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR0862853.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTT"
     gene            complement(1932..2828)
                     /gene="gluQRS"
                     /locus_tag="ACHUPG_003370"
     CDS             complement(1932..2828)
                     /gene="gluQRS"
                     /locus_tag="ACHUPG_003370"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR0862854.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWMLTAVPESAIVNSTFSNASC"
     gene            complement(2895..3350)
                     /gene="dksA"
                     /locus_tag="ACHUPG_003371"
     CDS             complement(2895..3350)
                     /gene="dksA"
                     /locus_tag="ACHUPG_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR0862855.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3528..4232)
                     /gene="sfsA"
                     /locus_tag="ACHUPG_003372"
     CDS             complement(3528..4232)
                     /gene="sfsA"
                     /locus_tag="ACHUPG_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR0862856.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4247..4777)
                     /gene="thpR"
                     /locus_tag="ACHUPG_003373"
     CDS             complement(4247..4777)
                     /gene="thpR"
                     /locus_tag="ACHUPG_003373"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR0862857.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4851..7280
                     /gene="hrpB"
                     /locus_tag="ACHUPG_003374"
     CDS             4851..7280
                     /gene="hrpB"
                     /locus_tag="ACHUPG_003374"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR0862858.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPKILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
BASE COUNT         1653 a         1906 c         2060 g         1691 t
ORIGIN      
        1 accatttgct taatttgtca aatgctcttg tatgtaagac ttcacgcaac gtctccccat
       61 caggaaacgc cagctccggt gcaatctcaa acagcggcca cagcataaat ccacgattct
      121 tcatatcgta gtgcggaacg gtcaggcgtt cggtatttat cacttcatta ccaaacagca
      181 tgatatcgag atccagcgtg cgtggtcccc agcgttcagc tttacgaaca cgaccctgtt
      241 gcagttctat gcgctgtgta tggttgagta actcttccgg ggcgggcgaa gtttcaagcg
      301 ccacggcggc gttgaggtaa tcgggttgat cttgcggccc cagcggtggg gtgcggtaaa
      361 acgaagaaac ggcaagaatg cggctttcag ggatatcgcc taatgctttc agggcagcat
      421 tgacctgctc cagcggagag gccagattgc tgcctatggc aatatacgcc actgtcatgt
      481 ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga gtacgacgac gcggtgacgg
      541 ttcttcatcc agctcgttaa gcatcccttt ttgatctggt ggtgcggaaa cctggaactc
      601 accccaccat ttcaccagac gctgcagttc agcattacgt tcaacttcag ctcgcaaggc
      661 caacaggtca taagccgcac ggaacttagg atgctccagc agtttccatg cgcgtttacc
      721 ctgacgacgg gacatacgca actgcaactg ccagatatcg cgggttaacg tcgtcagacg
      781 tttcgggatt gccagtgaac ggcaggcttc gtccagcacg tcgttcatcg ccagcgcgaa
      841 agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc tgtgccgtct ccagcagtgg
      901 gtaccagaac atggcggcaa acaagaacgc cgggttcacg cgcatatcgt tatggatacg
      961 cgtatcggta ttcttcagca cctgttcaat gatccgctcc atcgggctgt cgccattttc
     1021 cgtgaagtag cgggtaatgg tcgggaacag cggctggaac agatgatatt cacacaacag
     1081 cttataggtt tcgtaaccgt agcccgcttg tagcagttta agcgattctt caaacaggcg
     1141 tgccggtggg atatcgttca gcagggtagc gaggcgaggg atcggttctg cggtttccgg
     1201 gctgatgcgc atacccaatt tggcggcaaa acgtaccgcg cgcagcatac gtaccggatc
     1261 ttcacggtag cgcgtttccg ggttaccaat cagacggata acgccgtcct tcagatcctt
     1321 cataccacca acgtaatcac ggacggtaaa atccgctacg ctgtaataca ggctgttgat
     1381 agtgaaatcg cggcgctggg cgtcttcttc gatggagccg aaaatgttgt cgcgcagcaa
     1441 catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt cgtggtgtcc
     1501 acggaaggtc gcaacttcga taatctccgg gccaaacatt acatgagcca gacggaaacg
     1561 gcgacccacc aggcggcagt tacggaacag tttgcgcacc tgctcaggcg tggcgttagt
     1621 ggttacgtca aaatctttcg gctttttgcc aagtaacagg tcgcgcacgc cgccgccaac
     1681 cagccaggct tcgtatcccg ctttgttgag cctgtacatt accttcaggg cattttcact
     1741 gatatctttg cgggaaatag catgctgctc acgcgggatc accgtcacct gtggacgggc
     1801 gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa aattagcgac
     1861 tcgggtaaaa atagtacacc tcggtagtgt caaacatcat tcaggacaaa aaaatagcgg
     1921 ctaatcatag ctcagcatga cgcatttgag aatgttgaat ttacaattgc cgactcgggc
     1981 acggcggtca gcatccagtt tttgacggct gactgaagga tttgctcgac gctgaaatcc
     2041 tgccagtgtg cttctgcctg ctgccccaga aattgaagtg ccgcgattag taccgggcgt
     2101 ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc gccttgtgga
     2161 ttaagcgcca gcggcagatg aatgtaatct ggcactttcc agccaaaaag ctggtacagc
     2221 gggatttgcc ttactgttgg ttcaatcaga tcagccccac gcactatttc tgtaacgccc
     2281 tggaaatgat catcaaccac aacagccagg ttgtaggcga acaacccatc acggcgatga
     2341 atgataaaat cttcccgtgc cagtttttcg tcggcgtgaa taatgcctcg cagcaggtca
     2401 gtaaattgcg tgaccggatg ctgctggcgg atacgcactg cggcgttgtc tggtccatga
     2461 tgcaacaccc ggcaatgacc gtcgtaaata ccgccaatgc tttgaatacg cgcacgcgta
     2521 caggtgcagt aataacttag tccttgttca tgtaaccagg cgagtgcttc acgataggcg
     2581 tcgtgacgtt gcgattgcca gagaacatcg ccgtcccagt gcagaccgta atgttccagc
     2641 tggcgcagga tagtttctgc ggcaccggga acttcacgag gcgggtcgat atcttctatg
     2701 cgtaccagcc agcgaccttg ccgggcgcga gcctgcaaat agctgccgag cgcggcgatc
     2761 agagagccaa aatgaagctc gccggaagga gagggggcga agcggccaat atactgtgtg
     2821 tctgtcatct ctttgaacaa aaaataaggc gggagcattt cccgcctgtg gtaaacgtga
     2881 tggaacggct gtaattagcc agccatctgt ttttcgcgaa tttcagccag cgttttgcag
     2941 tcgatgcaca gatcggctgt cgggcgcgct tccagacggc gaataccaat ttcaacaccg
     3001 caggattcgc agtagccgaa atcttcgtct tccacttttt tcagcgtctt ctcgatcttt
     3061 ttgatcagct tacgctcgcg atcgcggtta cgcagttcga ggctgaactc ttcttcctgg
     3121 gctgcacggt ctaccgggtc cgggaagttg gctgcttcat cctgcatatg tgtaacggtg
     3181 cgatcgactt catccctgag ttgattacgc catgcttcca gaatacgacg gaagtgcgcc
     3241 agctgggctt cattcatata ctcttcgccc ggcttctcct gatatggttc caccccagcg
     3301 atggcgagaa tactcaggga cgatgtttta cggttttgcc cttcttgcat gttgcttctc
     3361 cttaacacgc actatcgatc cccatgttcg ggggaaaaat gaggccgcta taaatagcag
     3421 atgcttttcc ggatagcaat tatctaaacg taacacttga caactgtgtg aggaaaagcg
     3481 tatttgcgca cgcgaccaga atgtaaatta accagttact tactttacta caatgtaacc
     3541 ggcagtgatt ttttaagagc catgccttca gcagaaattt ccgctttgta agccagaatt
     3601 tctacccccc tctgttgagc ttctgacaat agttgcgcgt atttctcatc gatgtggcgc
     3661 gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga aaaaaataac cgcacgctgg
     3721 ccttcagccg ctacgctcat caactcccga aggtgtttct gacctcgttc agtgaccgca
     3781 tcgggaaaat atccctgttc gttctccgct aacgtaaccg atttcacttc aatatagcag
     3841 tctggacgcg aatccgcctg caacataaag tcaatacggc tgcgttcgga gccgtatttt
     3901 acttcgcttt tcagcgagct atagcctgac agttctgaaa ttgattcatt aaggatagcc
     3961 tctttcgtca acctgttagc ccaaagcgta ttgacgcaaa taaatgcgcc gctctggctt
     4021 tgagttaatt cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt cgaataccag
     4081 acggtatcgc caggcgttgc acaaccggtc atcgcacccg tattcgggca gtgtagcgtt
     4141 aattcgcgac catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg ctgaattagc
     4201 gtcgcgcgct gtagaggggg agaaaattcc attgcgacaa tccttgttat tgcgttagcg
     4261 cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag gcgtaaaggg
     4321 tgaactccgt caccgcataa gaccagttaa aacccggcgg cgggaatgtc accgcctcgc
     4381 tggcgtcgcg caataaggta atatgtggat gaaacggacg attgctttga aaacaaccgc
     4441 tgcgggcagc ctgtgaacgg agcatattcg ccagctggat taagccgcgt ggaggctgac
     4501 gcatccctaa ccacaccaca cgcgaacgca gccattgtcc ggcgtcatca agcgtgagtg
     4561 tgaaaccagg ttgacgaatc cgtccggcta aaagagaaag cgccttctct ttctctgcgc
     4621 tcacttcgcc taaaaatgcc agagtcaggt gcaaattatc ggcggcgacc ggacgtcccg
     4681 cctcaggtgg gaagtgtgtg gcgcgccaat ggataatctg ttcgcggatt tctgcaggta
     4741 agtcgatagc aaagaacaga cgttgcggtt cagacatgtg aggcactcgg ttatgaatta
     4801 ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt gtgtcgtcgt
     4861 tgcccgttgc tgccgtctta cctgaattac ttaccgctct cgattgtgcg ccgcaggtat
     4921 tattaagtgc gccgaccggg gccgggaaat caacctggct gccgctgcaa ctgctggcgc
     4981 atcccggcat taacgggaaa attatcctgc tggagccgcg tcgtctggcg gcgcgtaacg
     5041 tcgcgcaacg gctggcggag ctgcttaacg aaaagccagg cgataccgtt ggctaccgga
     5101 tgcgtgcgca aaactgcgtc gggctgaata cccgcctgga agtggttacc gaaggcgtgc
     5161 tgacgcggat gatccagcgt gacccggaac tgagcggtgt tggattggtg atccttgatg
     5221 agtttcatga gcgcagtttg caggcggatt tggcgttggc gctgttactc gatgtgcaac
     5281 aaggtctgcg tgatgacctt aaactgctga ttatgtcggc tacgctggac aatgaccgct
     5341 tgcagcaaat gctgccggaa gcgcccgtcg tcatctcaga agggcgctcg tttccggttg
     5401 aacgccgtta tttaccgctg cccgcgcatc agcgttttga cgaagccgtt gcggtagcca
     5461 ccgccgaaat ggtgcgtcag gaaagtggat cattactgtt atttttacct ggcgtcggag
     5521 aaattcagcg cgtgcaggaa caactggctt cgcgcatcgg cagtgatgtg gtgctctgcc
     5581 cgctgtatgg cgtgttgtca ctgaacgatc agcgaaaagc gatcctcccg gcaccgcaag
     5641 ggatgcgcaa agtggtgctg gcgaccaata ttgctgaaac cagtttaacc atcgaaggta
     5701 ttcgtctggt ggtggattgt gcccaggagc gtgtggcgcg ctttgatccg cgcacggggc
     5761 ttacgcggtt gattactcaa cgcgttagtc aggcatccat gacgcagcgt gccgggcgcg
     5821 ccgggcgtct ggagccgggt atcagcctgc atttaatcgc caaagaacaa gcagaacgcg
     5881 ccgcggcgca aagtgagccg aagatcttac aaagcgatct ttccggtttg ctgatggaat
     5941 tactgcaatg gggatgcagc gatccggcgc agatgagctg gctggatcaa ccgccaacgg
     6001 tgaatctact ggccgcgaaa cgcctgttac aaatgctggg ggcgctggag ggtgaacggc
     6061 ttagtgcgca agggcaaaaa atggcagcgc tgggtaacga tccgcgttta gcggcaatgc
     6121 tggttaacgc aaagagcgat gacgaagctg ctaccgcggc aaaaattgcc gccattctcg
     6181 aagagccgcc acggatgggc aatagtgacc tgggcgtggc gttttcgcgc aatcaaccag
     6241 cctggcagca acgtagtcag caactgttaa aacgcttaaa cgtacgcggc ggtgaggcag
     6301 acagttcgct tatcgcgccg ctacttgccg gggcgtttgc cgatcgcatt gctcatcgcc
     6361 gtgggcaaga tggacgctat caactggcaa acagcatggg agcgatgctc gatgccgacg
     6421 acgcgctaag ccgccacgaa tggttgatcg caccgttatt attgcagggc agcgcctcgc
     6481 cggatgcgcg gattttactg gcgctaccgg tcgatattga tgaattagta caacgctgtc
     6541 cgcagctggt gcagcaatct gacaccgtgg aatgggatga cgcgcaaggt acgctgaaag
     6601 cctggcgtcg gctacaaatc ggtcagttga cggtgaaagt gcagccgctg gcgaaaccgt
     6661 cagaagacga gttgcatcag gcgatgctta atggcatccg tgataaaggt ttaagcgtgc
     6721 tcaactggac ggcggaagcg gaacagctac gcttgcgttt gttatgcgcc gcaaagtggt
     6781 tgccggaata tgactggcca gcggttgatg atgaaagttt gttggcgacg ctggaaacgt
     6841 ggctgctgcc acatatgacc ggcgtacatt cactacgcgg cctgaaatca ctcgacattt
     6901 atcaggcact acgcggatta cttgattggg gaatgcagca acgtctggat agtgaattgc
     6961 ctgcgcatta cactgtgccg acgggaagcc ggatcgccat tcgttatcat gaagataacc
     7021 cgcccgcgct ggcggtgaga atgcaggaga tgtttggtga ggccactaat ccgacgatcg
     7081 cccaggggcg cgtgccgctg gtgctgaagt tgctttcccc tgcccaaagg ccgctgcaaa
     7141 tcacgcgtga tttgggggcc ttctggaaag gggcgtatcg tgaggtgcaa aaggagatga
     7201 aagggcgtta tcccaaacac gtctggccgg acgacccggc aaacacggca ccgacgcgac
     7261 ggacgaaaaa gtattcgtaa ggttggtttt ctccctctcc ctgtgggaga
//
LOCUS       DAZPAB010000183         7261 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZPAB010000183 DAZPAB010000000
VERSION     DAZPAB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.183"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..92
                     /locus_tag="ACHUPG_003375"
     CDS             <1..92
                     /locus_tag="ACHUPG_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862859.1"
                     /translation="GAVQRAFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(106..297)
                     /locus_tag="ACHUPG_003376"
                     /pseudo
     CDS             complement(106..297)
                     /locus_tag="ACHUPG_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            295..619
                     /locus_tag="ACHUPG_003377"
                     /pseudo
     CDS             295..619
                     /locus_tag="ACHUPG_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(649..3561)
                     /locus_tag="ACHUPG_003378"
     CDS             complement(649..3561)
                     /locus_tag="ACHUPG_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFR0862860.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            4044..4886
                     /locus_tag="ACHUPG_003379"
     CDS             4044..4886
                     /locus_tag="ACHUPG_003379"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR0862861.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4889..5977
                     /gene="capU"
                     /locus_tag="ACHUPG_003380"
     CDS             4889..5977
                     /gene="capU"
                     /locus_tag="ACHUPG_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFR0862862.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5982..6932
                     /gene="virK"
                     /locus_tag="ACHUPG_003381"
     CDS             5982..6932
                     /gene="virK"
                     /locus_tag="ACHUPG_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR0862863.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6997..>7261
                     /locus_tag="ACHUPG_003382"
     CDS             6997..>7261
                     /locus_tag="ACHUPG_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR0862864.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMF"
BASE COUNT         1938 a         1647 c         1733 g         1943 t
ORIGIN      
        1 ccggggcggt tcaaagggcc ttcccgagag tggtaaagga gaggccctgg aaatacccca
       61 cagccccgaa aaagagccag tcagttgctt aactgactgg cattaaggcc gccggggacg
      121 tttttttacc accgggagtt tgggcataac ggtgctttca gtaagtacga caggtactgt
      181 ggaggcactt ccgtaaagaa gggcatcaag ctcctgctcc gacatacctg caggcaaagg
      241 ccatgaaagc cctgatttcc ggaagcgagc gaacagtcca caaactgttg aacgtggtgt
      301 cagcggcgga cagggataag ccctcgtcag cagttccgtc agcactgcga cagcgttctt
      361 tacccggtca aaacagcgtt gcggtgcacc acgcctgacg gatgaactgc gtgctcaggg
      421 ttaccacttt aacgtaaaaa ccgtggcggc aagcctgcgc cgtcaggggc tgagggcaaa
      481 ggcctcccgg aagttcagcc cggtcagtta ccgcgcacat ggcctgcccg tgtcagaaaa
      541 tctgctggag caggattttt acgccagtgg tccgaaccag aagtgggcgg gagacatcac
      601 gtacttacgt acagatgaag ttcggttaca tcctgtcagc acggaacctc acgctttttg
      661 agcggcacga cggatccccc ccacccagta tccgacagac ttttcactgc gttcgtaaaa
      721 acccttcttc cctcccagca tatccactaa ttccggtgac atatcctgca gataagtctg
      781 caaatcatgc cccagatctt ccggtgactg caactcataa accgcgcgcc acacatcacc
      841 cttccttatt ttaagccccg ggttaagaat atactgtccg tggttcagcc tcagaaaaag
      901 cttttttcca taaggatttt tgctgttaac ttcatgttga gagagctggc tgctgatata
      961 actccttcgt ttgcggcgtt ccggcagcaa cgcttcagga agatgaacca cacactctgc
     1021 cagtcgtgcc gcactgaagg cttccgaaaa aaacagagca cttcccagat gcgtgtggaa
     1081 cagcgcctgc atcaggatca ccagaaacat tgccatctgc cggttgtcca gtttgatgag
     1141 gcgtccttcc acctgcagtg acagactttc aggtttcagc agggtataca actgcgcact
     1201 tttctgttgc gcataacggg gcgtactgac cgccgctgac agaacttgat gataagccgc
     1261 cagcccgtca ttaccggtca gcaagggatc tgcaccacgc tccagcagta actgaaccag
     1321 tgcaatatta cctgcccggg ctgctgacat cagcggagta agattaaacg gcgaacggtg
     1381 atcaacacca taacgttcaa tatcccgcaa cacggcaacc gggttattca ttgagtatgg
     1441 ccggaaatac ttcagctcca gtaactgcag agtcttcgtt ctgggctgcc ttgtgggctt
     1501 aaaatcttcc cggtacagtg cattatacag cgggaacagg gaataaatca gactgtactc
     1561 cagcgcctgc agttgtatgg ttttatcggc tgtacctgcg aggatatgct gtttccactg
     1621 tcgtgcatcc tctgcggtga taatctgcca tggcatctca gatgtctgca ggatccggct
     1681 acggatggtg tccgcctgct cctgcttccc ctgtttttca agcagatgtg cggttttctg
     1741 ccactcatca cggctggaaa tctcttcctg cagattcaga ggctcttcct gatgcgtgag
     1801 cgcaagcaga ctccacagag gatgctccac ctgctgttca acaagataca cattgtgttt
     1861 agcacgggtg agcgcaacat acagcgcgtt ggtaaaaaat ttatagattt ccgctgaacg
     1921 atcctgcctg tcccgcggac gggaataacg ggcctcgccc tcaagatcag ccggtgtaag
     1981 accctcgcaa atatcatcaa atgcctgccg ggcagcgctt actatattgt aaaggatcac
     2041 cgtttcgtat tccagcccct taacctcctg aaccgaaaag accagcggcg tactgaacca
     2101 gcatcgcgcc tgtgctttct gttccggatg catcacaatg accgctacac gattcgaaag
     2161 tgaggttttg gcattcagtt cctgctttgt ctcttcccta tcgtccagca accggatggt
     2221 cccttctgcc tgaccgcagc ttctgacaaa atggtgactt tcccgatcaa tagcactaaa
     2281 gcggacctgt ttgagtctca gcaaccggtt agccagtgca gtgacgtggt gcccgttacg
     2341 gtaattcgcc tgcaggatat taacggttgt gtcgtttccc tgttgctgcc ggaaaaagag
     2401 acttttgagc gaactccatg agaaaaagct cggatgaact atctgattgg catcaccaca
     2461 gagtaaaaac tgaccgggat gacgtagcgt tttcagtacc agttgcagtt gaacaggcgt
     2521 catatcctga acttcatcaa caaaaatgac atcataacgg ggagttgcca acgaaagata
     2581 ctgatgactg agcagattac tgtcatactg atgcgactgt ttcagccatg cgatataacg
     2641 ttcaaacagg acatagaccg tcgggcggtc ctccatgcca tagagcgact gccgtatccc
     2701 cagtgacaga tacgcctccc gggacagcgg cccattcccg gaggcaaccg caccgatcac
     2761 gcccctgaac tcctcataca gggtatgggc agcccccagt gcggcgatag ccctgttgcg
     2821 gggcagccag tcgctgaacg cactgaaagt cacctccctg ccttccggaa tgcgcagagt
     2881 ttccagaaat tccgtcagcg aaagaaagtc aatattctgt acctcactcc cttcccctga
     2941 agcgtcatag agagtacgcg ctttttccac cagaaatgag gaaaggctga gatacagtat
     3001 atcaccagca gcctgcttca ttttttccag taccagcgcc gtcttaccac ttcccgccga
     3061 gccaacaatc accagcggta gcgggtgttc gtacagtgcc tgctgatcat catcaaagga
     3121 cagcatttta tccaggcggt ggaaacgccc atgagacgga ttgatatacg tcagcgcttc
     3181 agtcgcgata tcaacaggat ccggcactgg ttgttgttgt aagcgccctt catcaatcac
     3241 cacgtttctg cgaagaaaac gtgaagtgtt ataggcatga ttgcgaatat attccagaac
     3301 aaggcagata gtttcaccgt ggtagcgata cagcgaaaac agtaatcggt cccggatact
     3361 gagccgggcg cggtaaagat tggtgtcaat tttacgaaca tcagcctgaa caaagttatc
     3421 ggtggcaata gcctgtgcaa attttttata acccgggatc gtgtcaggat tcagagtatg
     3481 ataaagacag attttcatgg ctgccggttt ccgtcgtgca aaaaacatgc tgattttacg
     3541 aaggaatgcc ttactgaaca accttgcccg gaataccccc cgaaatcaga tggaacaatc
     3601 agaaaaatgc tctgcagtta tggaataaag aaggtggggt ggtacgccgg gagggcgatc
     3661 cgctggaaat ggcgcagtag ctgcaggcct gtcatgatgc aggaatagaa gttcgtgtac
     3721 aggttaatga aagcgtcacc ccgtaaataa actctgcgct gaaattcctt tttttcattg
     3781 gagtacctgt gttgttctga ggtgaacata tcacctctgt tcaggtagcc aaattcagtg
     3841 tgccacctca aggttcttag tgagtcgttt ttaaaagata tgataatata tcattgttta
     3901 tgaattgttg atgtttaatt tagtaaatta tatcaaatat gccatgggtt tgctatgtta
     3961 ttcatccagt atctggaaat aaaatgcacc agctttattg ggttttcctt ttgtctctgc
     4021 ttgctaggtg ccagaaactt taaatgttaa acgaaggggg cattttgttc aaagccaatc
     4081 atgttccggt attaatgtat catcatgtca gtcattgccc cggactggtt acactttctc
     4141 ccgtgacatt ccggaagcaa ataaaatggt tggctgaaaa caactggaaa acactgtcat
     4201 cagatgaact ggagtttttt tatcggggcg gaaaactccc ccggaaaagc gttatgttga
     4261 catttgatga tggatacctt gacaactggt ttcaggtata tcctttatta aaagagttta
     4321 atctcaaagc acatattttt ttaattaccg gttttattgg gaatggtccg gttcgtcact
     4381 ctcctggaaa ggaatattct catcgcgatt gcgaacatca aatagcgaca gggaatgctg
     4441 ataatgttat gcttcgctgg tctgaagtaa acgaaatgtt gcaatctggt ctggtcgaat
     4501 ttcatgttca tacgcatact catacacgct gggataaaaa gttcagttca cgagaagaac
     4561 agtgtaaaca tcttcggcag gacttgttga gcgggaggga atatttgaaa gaaatgacag
     4621 gaaaatgcag taaacatttg tgttggccgg aaggttatta taacaaggat tatattcagg
     4681 tcgctgaaga acttggattc tattatttat atacaacaga aagaagaatg aatgctcctg
     4741 ctaaaggaac tacacggata ggaagaataa gcaccaaaga acgggaaagt tgcgcatggc
     4801 tgaaaagacg acttttttat tatacaaccc cgtttttttc atcgttgctg gcctttcata
     4861 aggggccaag attaccggat gattaataat gaatatacta tttacggaat catcaccgaa
     4921 tataggtggt caggaattac aggctgttgc tcaaatgaag gccctgaaga aaatggggca
     4981 ttcagttctg cttgtctgca gggaaaacag caaaattgct tttgaagcca gtaaattggg
     5041 gattgatatc acattcgcgt tatttcgaaa cagtcttcac atccctactg catggagatt
     5101 actcggaata gttcatggtt ttcagcccaa tgcaatcgtt tgtcacagtg ggcatgatag
     5161 caatattgtt ggtttagtac ggttattcac taggaaacat ccattcagaa ttatcaggca
     5221 aaagacatat ttgacacgaa aaacaaaagt tttttcaata aatcattttt gcgatgaggt
     5281 gattgttccc ggaacaagta tgaagacaca tctggagcag gaaggatgtc gaacccgggt
     5341 tactgttgtg cctccaggct ttgatttcca gaaattatac gttgattccc gaaacagttt
     5401 gcctccaaat gttctttctt ggctggcgtc ccgaagggga tgccccgtta ttgctcaggt
     5461 aggaatgttg cgcccggaaa aagggcacga atttatgttg aatttactgt tccatttaaa
     5521 aatgaatgga cgacagttct gttggttgat tgtggggtct ggttcgcctg aactgcggga
     5581 gcatttacag tatcagattg acagtatggg catgcatgat gatgttttta ttgctgacaa
     5641 tgtttttcct gccgcccccg tatatcgggt tgccagtctg gtggttctgc cttcagaaaa
     5701 cgaatcgttt ggtatggtac tggcagaagc atcggcattt tctgtgcctg tactggccag
     5761 tcagattggt ggaatccctg atgttattca gaataaccag accgggacat tgttaccagc
     5821 aggtaataag cacgcatgga tgtgcgccct gaatgatttt tttaatgacc ctgggcgttt
     5881 ttatcagatg gctcgccagg caaaacagga tatagaagag cggtttgata ttaataaaac
     5941 tgcgttaaaa atactcacat tagcgaagca caagtaacaa tatgttttct gtaagtaact
     6001 tatcatttat cggtttcctt aaaaggattg ttttttcctc agattcactc ccggggaagt
     6061 gggaacacag aaaattccgg ttcatgtaca ttttgcgatg tgctataaat ccggttgcca
     6121 gtattcgata ttattacgaa ctgcgttcct tgcagtgcat tgaggatatt ctggcaatac
     6181 aacccacgtt gccagcaaga attcatcgac cttatttgca taaagggggg cgagcctggt
     6241 cccgggggca atatattctt gagcattatc gctttgtgca aaatttgcca gaaaaatatt
     6301 ctgaattttt gtttccacaa aaatcggtat cattagtcca attcatcggg aaagatggag
     6361 aggattttga tatccagtgt tctcccagtg gttttgaccg ggaaggagag ttgatgttgt
     6421 cattgttttt caataaaata gtgattgccc ggttaacatt ttcagtcatt ctgactcaaa
     6481 atggacacac cgcttttatt ggcggattac agggagcccc caaaaatacc ggacctgata
     6541 ttattcggtg cgcaacccgg gcctgttatg ggcttttccc aaaacggatt atttttgaag
     6601 cattttgtgc gttgatgaaa gcttgtaatg tctctgagtg tctggcagtc agtgagcaca
     6661 gtcatgtttt ccggcaattg agatactggt atcagaaacg caaaaccttt gttgctgtct
     6721 acagtgattt ctgggagtct gtagcaggga aaacctgtgg tgactggtac aaattaccaa
     6781 cacaggtggt tcgaaagccg ctaagcaata tagctagtaa aaaacgctct gagtatcgaa
     6841 aacgatacgc tttgctggat tatatccatg aaactgctat ccgctctttg gatgcatatc
     6901 ctgtaaactc agaacatcag gacttaaatt aaggtttgat gtattctctg aataaaatat
     6961 taatgatgat tttggtaggg gcattcgcac taaataatga aaaaatacaa atccgagttt
     7021 attcctgaat ttaagaaaaa ttatctttcc cctgtttact ggtttacatg gttcgttttg
     7081 ggaatgattg caggtatttc aatgtttccc ccttcattca gagatcctgt cttggccaaa
     7141 atagggcgtt gggtgggcag attgagcaga aaagctcgtc gcagggcgac gattaattta
     7201 tcgctttgtt tcccggaaaa gagtgataca gaacgggaaa taattgtcga caatatgttt
     7261 g
//
LOCUS       DAZPAB010000184         7203 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZPAB010000184 DAZPAB010000000
VERSION     DAZPAB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.184"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(94..2097)
                     /locus_tag="ACHUPG_003383"
     CDS             complement(94..2097)
                     /locus_tag="ACHUPG_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862865.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2094..3230)
                     /locus_tag="ACHUPG_003384"
     CDS             complement(2094..3230)
                     /locus_tag="ACHUPG_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR0862866.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3223..5502)
                     /locus_tag="ACHUPG_003385"
     CDS             complement(3223..5502)
                     /locus_tag="ACHUPG_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFR0862867.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(5513..6601)
                     /locus_tag="ACHUPG_003386"
     CDS             complement(5513..6601)
                     /locus_tag="ACHUPG_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFR0862868.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1684 a         1936 c         1920 g         1663 t
ORIGIN      
        1 ggtgactctc accaggggct atataggata tgcctaatac cgtggcgtga gcagtccgga
       61 actggagtag aactcttagt aaaaagcact atttcatcct tgttgctgaa gcatggggaa
      121 taattgttcg caaagcaaaa caccgttatt cattgcttct acccgtgcct cgctttctgt
      181 attacgaaat tgtgccaaca catgtgccag ccgataaaaa cccaccgcgg tgaggtcatt
      241 cgccagcaac tctgcctgat taatagcgct ctgttcctga tagcgccagc cgttatggag
      301 cagttgaata agtaacgcct ggcagcgcat cagcaactga tgagcagtag acggcacagg
      361 cagaacgctg gcagaaggta gtgataccac cacaggcgca gtttctgcgt ccagcgccca
      421 ggcacgagtc tttgtcatca ttacctgtgg ttccagtgtc aattgcccat caacaaaact
      481 gacaaagcca gaaaccagac acacggggtc gtctgtttgt tgcaaaagcg ccgccatgcg
      541 ttcaatggca taaggcgcgc tggctgaggc tggcagtgat aacgtcagca gattatcttc
      601 accttcgccg ctgattacct gcgcatccag cgtctggcgg ctgctatccc aaccgagcga
      661 aatacactca gcgaccggca gaataaataa gttatcgacc tgattaagag gccgtatgca
      721 ggcgggggga cgctggcgta aatattcccg caaagccaca atgcccggct ggcgtaacgg
      781 cgcgctcaac atttgccagg catcaggcga cagcggtaca acgctgctta agcggttgcg
      841 ggtagctaac agcagctcgc catcggcact gcgttttgct gcttgtgaaa caatttgccc
      901 acccgccagt gcgccagcct gaaaactaaa cagccgacgc gtagctgccg gtgagtcttc
      961 ctgttcactt cgcggccaac tgcgcgaaag gtgcaaaata ctgccggtgt cgggatcggt
     1021 aaaccagatg cgtaaaccat aatgctcaat atcctgccag caacgcatac ctaaagacac
     1081 cagccgcaga tgatcaagct ttgcttctcc ggcaatgcca gagccaacga ccgtgcgcca
     1141 cggcacagga ggaacttcac caacactgtc gctccgggcc atctcttgtg cgcaatttaa
     1201 tcgactgttt aatgccgcaa gctgacgtaa gcattctccg gcatgatagt ggctggcgcg
     1261 ggcgtggaag gcatcaacgc tggcgcgcag ttgccgtagc gattcactca cccatcgcca
     1321 gctgcagcgt tccgctgctt gctgcgcgcg gctgaaagcg gcctcgtagt gaataagcgg
     1381 ctggctgatg ccgctcagcc ataatgcctg gcttagttgt tgaacatatt gacgacacgt
     1441 tttgccttct tcgctggcaa acggatcgtc agatgatgtg acgtgttcgc tgcgcatctg
     1501 ccagattaaa tgagtcaatt ccgcttgctg cgcttttgcc tggacaaagg cctgtactgc
     1561 cagtacgaca tgttcgcaaa gtgtgccttc aatacaatca caacgggcga aacgaatact
     1621 gctgcgggaa taaaaacgca catcgctcat cggtaagcgg gcagagggaa tttcacccgg
     1681 cgcacagaac aactcaatgg tgatgctttt agcgaccagc gcctgtgcgc gtttgcgggt
     1741 ggcatcggga agggtagcca gttcttccag ccagattgcc ggatcccatt cctcttcttt
     1801 tcccgtaggc tgggcggtag cacaaagtcg ttgataactt aacaccagca tcacgcgatg
     1861 acggcacatg ccgctggccc cataggtgca ctgagcctct ttcagtgcct ggccgttcgc
     1921 cagctgggta cggacaccgt cactgaaggt ggcgattaaa gcgctgttct catggctgat
     1981 ttccgggacg ttgccatttt ccagttcctt aaggctgcgc ttaacaaaac cggcattgct
     2041 taacgccgtc agtgcctgcg gtgtcagttc taataattcc ggacgtagtg aattcatgac
     2101 tgaagattct ccgcaagcca tgatgccagc tcgcccggtg tcatggcggc tatttgtgcg
     2161 ccgacattaa ccagcgcctg ggccgtatcg cggtcatagc aaggtgttgc tgtgctatcg
     2221 agcgctgcca gtcccagcac tttgatgccg ctctggacac actttttcac ttggtgcgtc
     2281 agcaatgatg atgaaccccc ttcataaaaa tcgctcacga ggataatgac gctttttgca
     2341 ggttgttcaa taagttgccg accatactcc atggcactgg cgatattggt tccgccgcct
     2401 aattgcactt tcattaataa ctctaccgga tcggcaacgt ctgccgtgag atcaacgacg
     2461 cttgtgtcaa acgccaccag atgggtacga atgccgggta actgccataa acaggccgcc
     2521 atcacagcag agtggatcac cgaatcgacc atcgatccgc tttgatcaac cagtaagacc
     2581 agttgccatt gctcgctttg gcgtttaatg cggctgttaa agcgggggaa ttcgatatac
     2641 aacttgccgt gttgcgggtg ccagtgttgc aggttggcgc gcagagtact tttgaaatca
     2701 aagtttcgcg ccagtggaat aaatgagcgg cgacgacgat cgcggacacc agaaaaagcc
     2761 tgacgaactt cctttgccag tcgcgccata atttcttcca caacctggcg cacaatcctg
     2821 cgggcggcag ccagtacttc ggggttcatc agatgtttgg tgtgcaaaac ggcgcgtagc
     2881 aggctttcgg aaggctgcat acgttccagc acgtcgagat ttgtcaccac atcttcaatg
     2941 ccgtagcgca gtacggcatc gctttccagc cgctcaatca cctgttgcgg aaacagcgtg
     3001 tgaatactgt tgatccactc aggagtggtg agatttgagc cacctaatcc actagagcgt
     3061 tcaccacgct ggagccgttc aggatcgcgc ccatacagcc actccagcgc gtgatctatc
     3121 tgccgggcgt tgtcatccag cccacaaagc gtcgtttctg ccgcttcgcc aagaattaat
     3181 cgccagcgtt gtagctcacg ggtggtcaga agatcgttca gttcagacat ggaaaactcc
     3241 ccagtgttgc agcgatgcca gtgcctgctg ttcgagttgt tgatgatgtg caatggcttg
     3301 cggtggacaa tgcaacggca tttgcagtgc cgaaacggga agttgcgcca gttgataatg
     3361 ctcaagcacc tgatgcgcca gcgtcccgcg ttctcgtggc ggtagccagg ccattgccgc
     3421 gcgtaaatcg ggcagggcat tggtaaaatc ggcatcactt agttgattta aatgactgct
     3481 gaaaccggcg ataaatgccg gctgacaggc cagttgatga cgggctaatg ccagcaaacc
     3541 gtgcagcgcc tcgccggatt gtgccgggga taactgcgcc agcatcgtca gcgccgcttc
     3601 ggcactagcg ttgggatgtt ccagacgcat cagcgcacca agagtcgcgc cgcgatccag
     3661 tgccaggaca tgaatcgcct gactgtgtcg ctccagcaag gctaccgccg cagaaagcga
     3721 aacgccgggg agttgcacac cgctatgtag atcgcgcagg atatgacaca acgcttgcca
     3781 gctatgcaag tgagcgtgaa actccttttc atccggtctg ccgttggatt cacacagcca
     3841 cagcgtgcga tcgatagccg cgcataacgt cgtttgtaat atctgcgcgc cttgcatacc
     3901 gctaatttca tccagccgcc ataaggcata tagcacttcc agcgcctggc ccatttcggc
     3961 aaattgattt tcctgggcga ttaataatgt gagttgctcc agtaattgtt gactgaagga
     4021 cgccagaccc gctgacgccg cttggctaag acatgtggta atgctgccga ttccacccgc
     4081 gtccagcata tcggcttcta atttatggcg tgcggcttcc aggagtgtgg caccaaagca
     4141 ggcggcctca atcaatgccg catgctggct aagcgggcgg gttaatttcc agcgttcttc
     4201 accgttgcct gcaagtgtca gtgtgcttcc ctgctggcgt acaatccctg ggatctccag
     4261 tattgccagc cgatgtaaca cctgactttg cgctagtcca ttggggttaa agcgatttaa
     4321 ggtaagctcc gccggaagag agattgcggt acgttccagt tcgcaagtga catcttttgg
     4381 cagaggcggt tgtggcgtag aaggggcaag tttaccgaat ccgtcacccg ctaatgtgtc
     4441 tatcaacgtt agcagaatcg gatcggtatc gggatgaata acgccgcgat agctccacgg
     4501 caacggcgca ttcagggctt ctttaatcag cgatcctgct atggcatcca gccagtcagt
     4561 gcgtaacggt agtgtatgac cgcgcaactg tgccagtgcc atcgcatgca gatgagcggc
     4621 agccatatcc gcggtcgaag caggcaactt gtgctggcgc aaacgggtga gaaccgtttt
     4681 tagcagttgt tctcccgcct gctgtaagcc ccactgccag caccagtttt gccagaccgg
     4741 ggcaggcatt cctgaaaggt atcctgccag cacatcaagg cgcttttcac tgtagggggt
     4801 gagataacaa cctgtaacgg catctgccag cgacggcaat tctggcgtgt taattacctg
     4861 cggacattcg cgccacattt ttgccagtgc cggagcgtgc cagccaccgc agacaactaa
     4921 cacgtcgcca ttattttgct gcatcgccca tccaatccag cgggccataa aggcttcgcg
     4981 ctgacgattt agagcatcgc cgggggagtc gccccgcagt tgggcaaaat agtgcgccag
     5041 tgcagagggt aatgcagttt gctggctttc atcttcgaac aagtgatccc acagggtgtc
     5101 gctgttatcc atgcgggtgg cacgcagcag caaggtctgg ctttcctctt gcgtatcagg
     5161 cgaatcgtcc tcttcttcac tctgcgccca gcaaggcaaa tcgatgaagt aagtttgtgc
     5221 ctgaatacga cgatcggctt gtagcgcctg ccactccggc gaaaattcag caaatggcgt
     5281 ccaggcacca cgcccggggg ctgcaccgtc ctgatactgg caataactgt aaatagctac
     5341 cggaagctgg tgggctaaaa acagttcgtc tacccggtca ttaaaatcag cagggccttc
     5401 aatcaacacg tatcgtggcc gctggctttc gattaacgat ttcaccagcc gggcgcaggc
     5461 cggactatga tgccggatgc cgacaattaa cggctcgctc atgcatcttt cctcacggca
     5521 ggcggtggcg ggcttgataa taagcctgcc agtgagcttc tttatgtgtg gcgacacgct
     5581 gttcgaagta acggcgcaga cgagcgcgat cttcttcgtt atctttgacg atggttccgg
     5641 caatgcagtc caccaggtcg gctggctcac ctgcgcgatt cgctaaaaac caggcgcgga
     5701 cgcctacggc gtgggccacg ttcacggctt cagcggtgga cataatcgct gttagtgtat
     5761 ccattgacgt tttcttttcg ccgttggcac gcagatcgcg gaaggtgcgg accagtaact
     5821 ccagtaccgc gtcaggcact ttatgcggaa taccgctatg ggccagcaaa cgcgccgaag
     5881 cactggcgac aagctccagc tcctgggcga aatccataat cggaaagaca gtttcaaaat
     5941 cgaagcgacg ctttagcgca gcactcattt cattgacgcc gcgatcgcgg gtattggcag
     6001 tggcaataat attaaagcct tctcgcgcat agagctgact ggcttcacca gtgagttccg
     6061 gcaccgtcat caccctgtca gaaagcatac ctaacagaca gtcttgcact tccagcggtg
     6121 tacgcgttat ctcttcaaaa cggacgattt tgccgtcgcg cattccctgg taaagcgggg
     6181 cagggaccag cgcttccgtt gatggaccat gattgataag cagggcgtag ttccagccgt
     6241 acttgatttg atcttcggta gtagatgcgc cgccctggat ggttaacccg gcatcgccgc
     6301 taatagaggt tgccagtaat tcagacagga gagattttgc ggtgccaggt tcccccacca
     6361 acatcaaacc gcgtccggtg gcaagagtga ctaacatacg ttcgacagcg gagggactaa
     6421 tcaccacttt acggctaata ttttgcgcgc tatcgccaag gataaaagca cgggccgcag
     6481 gcaaacttaa ctgccaaccg ggcgggcaag gtgcgttatc attttgtttt aattttgcca
     6541 gttcatcggc gtataacaca gcagcaggcg gacgctgcag atgattattc tgcggtgaca
     6601 tatattcatc cctgaaatgt attaatgaaa taaacgatat tttacgcgca ataaatgttt
     6661 ggcgcattag ttgtttgagc gaaaaatatc tggaaagcag atcacagtaa ataaaaaaaa
     6721 ctgctggtga ggcaggttct tttatttact attttcaggc ctaacaccac tgcctgtgca
     6781 acatcgtgtt ccaacgaacg aattataacc acctctggac agtgttgcaa ttaagataat
     6841 gagatttata atcctgactg ttgggaagtt gcgccggatc gaacctattt caagtcctgg
     6901 ctaccggacg ggtggtgccg caggtagtgc tctgatccgg agtgcaacat ggcgactcaa
     6961 caaacgtaaa ccagtatcga caggcagtgt aagggtaagg tgctggtgtt gtgctcctta
     7021 ataaggagag cggatggaca cgctgacaca aaagttaacc gtgctcattg ctgtactgga
     7081 gctattagtg gctctgttac ggttgattga tttgttgaag taacgggcaa attgaaacgc
     7141 atctaagggg gaataccgtt ccccccttta ataaagctat actcgtcata cttcaagttg
     7201 cat
//
LOCUS       DAZPAB010000185         7183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZPAB010000185 DAZPAB010000000
VERSION     DAZPAB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.185"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(371..1198)
                     /locus_tag="ACHUPG_003387"
                     /pseudo
     CDS             complement(371..1198)
                     /locus_tag="ACHUPG_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACHUPG_003388"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACHUPG_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFR0862869.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="ACHUPG_003389"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="ACHUPG_003389"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFR0862870.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="ACHUPG_003390"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="ACHUPG_003390"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFR0862871.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="ACHUPG_003391"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="ACHUPG_003391"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFR0862872.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="ACHUPG_003392"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="ACHUPG_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7183
                     /locus_tag="ACHUPG_003393"
     CDS             7090..>7183
                     /locus_tag="ACHUPG_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HFR0862873.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1676 a         1812 c         1876 g         1819 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gtattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgc
//
LOCUS       DAZPAB010000186         7140 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZPAB010000186 DAZPAB010000000
VERSION     DAZPAB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.186"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..151)
                     /locus_tag="ACHUPG_003394"
     CDS             complement(<1..151)
                     /locus_tag="ACHUPG_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862874.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLN"
     gene            229..1323
                     /gene="ddlA"
                     /locus_tag="ACHUPG_003395"
     CDS             229..1323
                     /gene="ddlA"
                     /locus_tag="ACHUPG_003395"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR0862875.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1347..1559)
                     /locus_tag="ACHUPG_003396"
     CDS             complement(1347..1559)
                     /locus_tag="ACHUPG_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR0862876.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1819..2127
                     /locus_tag="ACHUPG_003397"
     CDS             1819..2127
                     /locus_tag="ACHUPG_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR0862877.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2186..3280)
                     /gene="yaiW"
                     /locus_tag="ACHUPG_003398"
                     /pseudo
     CDS             complement(2186..3280)
                     /gene="yaiW"
                     /locus_tag="ACHUPG_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
     gene            complement(3293..4513)
                     /gene="sbmA"
                     /locus_tag="ACHUPG_003399"
     CDS             complement(3293..4513)
                     /gene="sbmA"
                     /locus_tag="ACHUPG_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR0862878.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4865..6022
                     /gene="ampH"
                     /locus_tag="ACHUPG_003400"
     CDS             4865..6022
                     /gene="ampH"
                     /locus_tag="ACHUPG_003400"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR0862879.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6023..6526)
                     /locus_tag="ACHUPG_003401"
     CDS             complement(6023..6526)
                     /locus_tag="ACHUPG_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0862880.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(6614..>7140)
                     /locus_tag="ACHUPG_003402"
     CDS             complement(6614..>7140)
                     /locus_tag="ACHUPG_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0862881.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         1812 a         1825 c         1726 g         1777 t
ORIGIN      
        1 aattaagaga aaaaaactca ccgcattgcc atttaagcca ggaataaatt agcttcccgt
       61 tcatcatttt tgtgatcttg cgcacatctt tgagataacg cactttcttc accagtacgt
      121 tgcacgcttt gtcagcagaa gattgcaaca atctttcaga aggccgcgaa tcccccgttt
      181 tgtcgtggta atgtatgcac tttgtgttag acgggatagg tttttaagat ggaaaaactg
      241 cgggtaggaa tcgtttttgg tggtaaatca gcggaacatg aagtgtctct gcaatcggca
      301 aaaaacattg tcgatgccat tgataaaagt cgcttcgacg ttgtgctgct gggcattgat
      361 aaacaagggc aatggcacgt cagcgatgcc agcaattatc tgctaaatgc agacgatcct
      421 gcccatattg cgttgcgccc ttcggcgacc agccttgcgc aggtgccagg taaacatgag
      481 catcagctta tcgacgcgca aaacggtcag ccgttgccga cggtggatgt cattttcccg
      541 attgtccacg gtacgctggg cgaagatggt tccttgcagg gaatgctgcg ggtcgccaat
      601 ttaccgtttg taggttctga tgttctggct tcagcagcct gtatggataa agatgtcacc
      661 aaacgtctgc tacgcgatgc cgggctgaac attgcgccat ttattaccct gacgcgcgct
      721 aatcgtcaca acatcagttt tgccgaagtg gagtctaaac tggggttacc actgtttgta
      781 aaaccggcta atcagggctc ttctgttggt gtcagcaaag taaccagtga agaacagtac
      841 gcaattgccg tcgatctggc gttcgagttc gatcataaag tgatcgttga gcaagggatc
      901 aaaggtcgtg agatcgaatg cgcagttctg ggcaacgaca atccgcaagc cagcacctgt
      961 ggcgagatcg tactcaccag cgatttctat gcctacgaca ccaagtacat tgacgaagat
     1021 ggcgcgaaag tggtagttcc ggcagccatt gcaccagaga tcaacgataa gatccgggcg
     1081 attgccgttc aggcttatca gacgttggga tgcgcaggca tggcgcgtgt agacgtgttt
     1141 ttaaccccag agaacgaagt ggtgatcaac gagatcaaca cactgcctgg cttcaccaac
     1201 atcagtatgt atccgaagct gtggcaagcc agcggtctgg gttacaccga tctgatcaca
     1261 cgtttgattg agctggcgct ggagcgtcac gctgcggata acgcattgaa aaccacaatg
     1321 taatatttta acgcccgata atattcttat tgggcgtttt cagtttcttc atcgcgacga
     1381 cgaataatca accccgccag ccagaaacta atcacccacg tcaccagacc gacggcatag
     1441 gtctgccaac cttttgcttc aaatcccagt acccccacca cgccattaag aatgaatata
     1501 aggccaatgg cgaacgcata gtagtgccag tcacggcgga tttttacagg cagattcatg
     1561 gcagctccgt taacgaaaaa caacatcctt gcatattctg taacaaccgt ctgtggtgta
     1621 gacaacattt tcgtaattat taataaattt aacttaaggt tgccattttg tgactcacaa
     1681 cgaagattca cgcttcggga atattcccaa catcaattta ccatgattct gatattgaca
     1741 atctaaatga cctgtcagac ttgccataag ccgctggtat cacaccagaa gagaagcaga
     1801 tgacttccgg aggtttttat ggctgatttc accctgtcaa aatcgctgtt tagcggaaaa
     1861 tatcgcaatg cctcttcaac gcctggcaac attgcctatg cgttgtttgt gctgttttgc
     1921 ttttgggctg gggcgcaatt gctgaacctg ttagtgcatg cgcccggcgt ctatgagcgt
     1981 ttaatgcagg tccaggaaac aggtcgccca cgggtggaaa ttggtttagg tgtcggcacc
     2041 attttcgggc tgatcccgtt tttagcaggc tgcctcattt ttgcagtggt ggcgctatgg
     2101 ctgcactggc gacatcgccg ccagtaaaat ttacgttgcc agatgcggcc tgaaacgcct
     2161 tatctggcct gacattcctg tttgatcact gtttcataca acgcgcccgc cgttcgtcta
     2221 cgcgcttcgc aaaccaggcc gtagtcaggt tgcgggtgat tttcgggctt tccagttgaa
     2281 tgccaggcaa catttcgcgg ggtagtgatt tacccgtttt cgcttcggca agttgataaa
     2341 ctttcttgta cagcgccgtc tcctcaaaag agaagctgtc acctttctct aactgacggc
     2401 gaatttcgct gtcgctcatc cccagttttc ccgccagttt gcgcgttgcc agttcggttt
     2461 tcccgggttc tttactgtcg taacgaatta aatcaccgtc cagtgccagc ttcacgccgc
     2521 tggccttact gactgcgttc tgaaacgcgg cattacggct ggcgtaccag ccagcgttaa
     2581 aatcagcaaa gcggtatatc ggtgcgctat agctggcggg ataattcagt aaatggtaag
     2641 taccaaacca caacccgccg cggcgactga agacttcctg acgaactgta ccgtccattt
     2701 tccacggata ccctttggta tgctgttcgg caaaagcaat gctgacctgc atcggcccac
     2761 cggtgcgcac cggattaagc gaaccaaaca gcgtctgccc cattggtacc atgctgatca
     2821 gatcgtcaaa aatcgcgctc aattgctttt ctgtacgcac cgaatctaac cgttcgctat
     2881 aacttttgcc gtttggcgat ttgattttta gcgcggtatg aaccagaaaa gcaggaatgt
     2941 gcatccgttc agcacgacgg tcaatctctt gctaggcgat cttgcttaag cccggaacgg
     3001 ccggatctgc ctgataattt gactcctgct gcgccaccgc cagcaccgag tagacgtttt
     3061 ccagcgtcgg tgccagcccc tgactttcaa aggtggtggc gagatctttc gcccaggcat
     3121 cgcggtcttt cacgctcgca ggcatcttct gccgcacgac gctcgctaca tctatcgctt
     3181 tttcgccctt tttcaacggt tgcggagcct gactactaca ccccgctaaa accagaaccg
     3241 ccaacaatga cagcgaagat agatgattga cgcgcgacat tacttctcct ttttagctca
     3301 aggtatgggt tacttcctga attttgtcac catccagctc atgttcaaag ctgcgcagac
     3361 gtttgtagat agacatcaac tcaaccagtg tggtccatga gttaatcagg tactggaaag
     3421 caccgcgaac ctgaccaaaa acgttggtaa tctgcgtcat caggccgagc gtaatcgtac
     3481 cggcaacaat tgacggaaac agcaagaaca aaccgaaaac gttatcgacc tgcaaataga
     3541 gaatgcgggc gatgttgaaa tacatatagt gaaaatagag gcgaaaatag tttttccgta
     3601 cggcgctaaa cagctcgcgt accgtaggcg gcgtcgcgcg cgtggcatcg tcttcaccat
     3661 aaaccagctc tttacggtag gcggcctcta ctcgctggtt tttaaactcc agccccggca
     3721 gtttgatccc taccactgcc agcaatccgg tccccatcag cgaccagacg atagcggcaa
     3781 tcaccagacc atacggaatg tgcccgataa tcggcagctc cggcacatgc gcggagagcg
     3841 ttaccagcac cggcaggaag gcgatcaacg tcatgatggc gttgataaaa ctgacgccca
     3901 tattctccag cgttgaagca aaacgcatgg tgtcttcctg cacacgctgt gcggcacctt
     3961 cgatatgacg cagttgttgc cagttcgcca tgtaatattc gttcatcgcc gtacgccagc
     4021 ggaacacgta gtgactgaca aagaagttgt tcagtacact gatcaccacc gcgatcagcg
     4081 caatccccag aaagacgccc acttcgcggt aaaattgttc gatagtgact ttatgcggcg
     4141 aacttagcgc ggtttgaatc agatcataga acggcgcata ccaggcgttg acggcgaccc
     4201 cgacttccac caaaaaccaa gtgacgaaga taatcagcgc agtaccgagt attgaccagt
     4261 attgccaacg gtgcgggctg tagataaacc agaaaaatgc aaaaagtcct acgcaaacaa
     4321 tgtagtaagc gtaaaaaatc aggaaatcca acgaccagaa acgcgcggcg ctaatcggga
     4381 tctgcccgga agcgccggtg atacgtgcca cccagtcacc cccaccggct tgccagaaga
     4441 taacggcaat caatgcccaa acaaaggccg agagaaaaaa cgttcccggc tttgggaaaa
     4501 aagacttaaa catatgcact cctgctaact tcttatcgtt ttaacaactg ctgtgtatta
     4561 accgcatgac cgctacaaag cgtcagccaa atgaaaccgc ccattgtgac ccatagcaag
     4621 gcgcttagtt cgccagcctg accataagaa ttgtttcgac ggatttcaca aaccaaagat
     4681 tgtttacata agcggattaa cacgcgcagg atcacaaatg caggcattgg tgagcaggaa
     4741 aaagatcgtg tttacgcgcc gttacgtttt taacactatt attgcactaa ttcgcccttt
     4801 gcaatccatc aatgagtagt ataaatacgc tcagttacct tcattcaatc tatggacacc
     4861 accgttgaaa cgtagtctgc ttttttctgc cgtgctgtgt gcggcgtcat tgacctccgt
     4921 ccatgcggcg cagccgatca ctgaaccgga gtttgcctct gatattgtcg atcgttatgc
     4981 cgatcatatt ttttacggca gcggtgccac ggggatggcg ctggttgtta tcgacggtaa
     5041 ccagcgcgtc tttcgtagtt atggtgaaac gcgacctggt aataacgttc gcccgcagct
     5101 ggattcggtc gtgcgtattg cttccctcac caaactaatg accagtgaaa tgctggtgaa
     5161 attgctcgac caggggaccg taaagttaaa cgatccgcta agtaaatatg ctccgccagg
     5221 cgcacgcgtg ccgacctaca atgggacgcc cattacccta gtgaatctgg caacccatac
     5281 cagcgccctg ccccgtgaac agcccggtgg cgcggcacat cgtccggtgt ttgtctggcc
     5341 aacgcgcgag caacgctgga aatacctttc tacggcgaag ctgaaagccg cgccaggcag
     5401 tcaggcggcg tactctaacc ttgcgttcga cctgctggca gatgcactgg cgaatgcgtc
     5461 cggcaagcct tatacccagt tgtttgaaga gcagattacc catccattgg ggatgaaaga
     5521 caccacctac accccctcac cggatcagtg ccgccgttta atggttgctg agcgcggtgc
     5581 cagtccgtgc aataacacgc tggcggcaat tggcagcggc ggcgtctatt ccacgcctgg
     5641 cgatatgatg cgctggatgc agcagtattt gtcatctgat ttttatcagc gcagtaacca
     5701 ggcagatcgc atgcagacgt tgatttatca gcgcgcgcag tttacgaaag tgattggcat
     5761 ggatgtcccc ggcaaagcgg atgcgcttgg tttaggctgg gtatatatgg ccccgaaaga
     5821 gggtcgtccg aggattattc agaagacagg cggtggtggc ggtttcatta cctatatggc
     5881 gatgatcccg cagaagaata ttggcgcgtt tgtggtggtc actcgttcgc cgttgacgcg
     5941 ctttaaaaat atgagcgatg gcatcaatga tctggtgacc gagctaagcg ggaataaacc
     6001 gttggttatc cccgcatcct gattaatact ctgaaggcaa acggttgata gcgaccagtt
     6061 tgcctttatt catttcgata tagccgcctt tacgcaaagc agcgagaact tctgcgacga
     6121 cagaacgcga tatgcgtgta cgttgatgga tataattcat cacaccaata cgtgatcgca
     6181 attcttcatt ccagtcaatc attgataata atgttgcgcg ggtttgtttg gctggtagat
     6241 gatatcccgt acaatttcct tctgatatta atttgtatgg tatatcgttt ttcattaaac
     6301 catcagccag ccccataata taaggagcct gggtaatacc gataagtacg ttttcttctc
     6361 tacgcagaga gataactcct tctaaaataa cgaaagtatc ttcgttattg acatcactgg
     6421 aaaatataac ttgcttttca ttattaaact cgaagcgcgt accgtatctg gacaaacatt
     6481 tatcgagctt accaaattcc tgaagaggtt taactacaga taacatttgc gcgtcctttg
     6541 cagtaatgcc cgtcaaatcc ttgacgggca ttatttagat taaattacca gtatttcttc
     6601 ggagtgaaga atattaccaa gtatatttaa cacccacgtt cgcggaccag tcttgatcta
     6661 cgtcaccacc accgaggtag ttagcatcgg tataggcgct gaagttcttg gtgaagctga
     6721 actgagtacc cagaccaaca cgtaccgcag acccttcagt accgttatcg atggaatcac
     6781 cgttcacatc gttatcgttg ttagagtcgt cgtagacgta agccagtttg aagtacggag
     6841 tcagagcctg atcttcgctg taggtgaagg tataacctgc atctacaccc agttcataac
     6901 gcatgctgtc gtaaggctga ccgtcaactt tcatgtcgtt gctcagctgg tagtcatcac
     6961 cagactggaa cagaccagaa atgctgccgt aaggagtcac gtaaccagca tcacccagtt
     7021 tgaagtcgta accggttttc aaaccgaagc cccaagcgtc ggagttggtg ctaccgtcaa
     7081 cgtaagtacc gttgctcatg gttgcagaca ggtcgttgtt gaagtgagag tagctcaagc
//
LOCUS       DAZPAB010000187         7113 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZPAB010000187 DAZPAB010000000
VERSION     DAZPAB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.187"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            121..1692
                     /gene="garD"
                     /locus_tag="ACHUPG_003403"
     CDS             121..1692
                     /gene="garD"
                     /locus_tag="ACHUPG_003403"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR0862882.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(1744..2568)
                     /gene="agaR"
                     /locus_tag="ACHUPG_003404"
     CDS             complement(1744..2568)
                     /gene="agaR"
                     /locus_tag="ACHUPG_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFR0862883.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            2817..4097
                     /gene="kbaZ"
                     /locus_tag="ACHUPG_003405"
     CDS             2817..4097
                     /gene="kbaZ"
                     /locus_tag="ACHUPG_003405"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFR0862884.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            4120..4593
                     /gene="agaV"
                     /locus_tag="ACHUPG_003406"
     CDS             4120..4593
                     /gene="agaV"
                     /locus_tag="ACHUPG_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFR0862885.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            4604..5383
                     /gene="agaW"
                     /locus_tag="ACHUPG_003407"
     CDS             4604..5383
                     /gene="agaW"
                     /locus_tag="ACHUPG_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFR0862886.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            5373..6182
                     /gene="agaE"
                     /locus_tag="ACHUPG_003408"
     CDS             5373..6182
                     /gene="agaE"
                     /locus_tag="ACHUPG_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFR0862887.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            6269..6703
                     /gene="agaF"
                     /locus_tag="ACHUPG_003409"
     CDS             6269..6703
                     /gene="agaF"
                     /locus_tag="ACHUPG_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFR0862888.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            6700..>7113
                     /locus_tag="ACHUPG_003410"
     CDS             6700..>7113
                     /locus_tag="ACHUPG_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR0862889.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         1684 a         1849 c         1894 g         1686 t
ORIGIN      
        1 acattgatca catttctgcg tttaaactcc tgacattctt atttcaccca atgaaatcat
       61 ttatttttaa atgagaccag gtcctcattt taataacccc tggctggaga atattgcaca
      121 atggccaaca tcgaaatcag acaagaaacg ccaactgcgt tttatataaa agttcacgac
      181 acagataatg tggcaattat tgttaatgat aatggcctga aagcaggaac gcgttttccg
      241 gatgggctgg aattaattga acatattccc caggggcata aagtcgcatt gctggacatt
      301 ccggctaatg gtgaaattat tcgttatggc gaagtgattg gttacgccgt gcgtgcaatc
      361 ccacgcggaa gctggatcga cgaatcaatg gttgtactac cggaagcgcc gccgttacac
      421 acgctgccac tggcaaccaa agtcccggaa cccttaccgc cgctggaagg atacaccttt
      481 gagggctatc gcaatgccga tggcagcgtg ggcaccaaaa acctgctcgg catcaccacc
      541 agcgtccact gtgtggcagg cgtggtggac tatgtagtaa aaatcattga acgcgatctg
      601 ctaccgaaat acccgaacgt cgatggcgtg gtggggctga atcatttgta cggttgtggc
      661 gtggcgatta acgcaccggc agcagttgta cctattcgta ccattcacaa tatttcgctg
      721 aaccctaact ttggcggcga agtaatggtg attggcctgg gttgtgaaaa gttgcagcct
      781 gagcgcctgc tgactggaac ggatgatgtg caagctattc cagtagaaag cgccagcatt
      841 gtcagtttgc aggatgaaaa gcatgtcggt tttcagtcca tggtcgagga tattttgcag
      901 gtcgccgaac gccatctaca aaaactgaat caacgtcagc gagaaacctg tccggcttca
      961 gaactggttg tcggcatgca ttgcggtggc agcgatgcgt tttctggcgt aacggcaaac
     1021 ccggcggttg gctatgcgtc tgatctactg gtgcgctgcg gcgcaacggt gatgttttca
     1081 gaagtcacgg aagtgcgtga cgcgatccat ctgctgacac cacgcgcagt gaacgaagag
     1141 gtcggcaaac ggctgctgga ggagatggag tggtacgata actatctcaa tatgggaaaa
     1201 accgaccgca gcgccaaccc ttcgccgggc aacaagaaag gcggtctagc aaacgtggta
     1261 gagaaagcgc tcggctccat tgctaaatcg ggcaaaagtg caattgttga agtgctgtcg
     1321 cccggtcaac gcccgactaa acgcggatta atttacgccg cgacgccagc cagcgatttt
     1381 gtctgtggca cgcaacaggt ggcttcgggt atcaccgtgc aagtgtttac gaccggtcgt
     1441 ggtacgccgt acggcctgat ggcggtaccc gtcattaaaa tggcaccccg caccgagctg
     1501 gcgaaccgct ggtttgattt aatggatatt aatgcgggca ccatcgctac cggcgaagaa
     1561 actattgaag aggagggctg gaagttgttc cactttattc tcgacgtcgc cagcgggaag
     1621 aagaaaacct tctcggatca atggggactg cataaccagc tggcggtgtt taacccagca
     1681 ccggtgacct gataattcaa ttcgtattat gcccgatagc acacgtttat cgggcatatt
     1741 gagtcacagc gaagacgcgt gatccccgac cagaatcact tcaaccccag cctttcgcag
     1801 tccttccaga ctatccgcag gaatgccttc atcaacaatg atcatgtcga tacgttgagt
     1861 atcaatgatc ttatgtaaac tggaacgatt gaatttactg gaatcggtga ccacgatgat
     1921 ccgttccgca acttcgcaca tccgacggct taaacgggct tcatcttcat tatgtgtgct
     1981 gacgccgcgc tccagatcga tcgcatctac accaagaaac agcatatcga agtggtaatt
     2041 ttgcagcgat tgctcagcct gatcgccgta aaaagattgc gactgacggc gcaaatgccc
     2101 gccggtcatc agcagctcaa cgccttccgc ttccagcaac gcattagcca cgttcatacc
     2161 gttggtcatc gcaattacgt cagtgtactt gcgcatcaga cgagcaatct caaaagtggt
     2221 ggtcccggaa tcgaggatca cccgatgacc tggctgaatc aactcaacgg cagctttcgc
     2281 aacgctgcgt ttcatcgcgg tgttcagtgc gcttttatct tccactgatg gctcgactga
     2341 cggcgtcgtg ctatcgcaga tcaacgcgcc accataggca cgcacagcga tcccctgctt
     2401 ttccagaaac gccagatcat tgcggatcgt cacagtagat acgccataca atgccgacag
     2461 atcgttaacc tgcacactcc cttgctgtcg cagacgctga atgatctgtt ctcgtcgctc
     2521 gctggtgcct gtcactcgct tctcacctga agcgtcggta ttactcatag taagtccttt
     2581 cgtaaaactt tcgtttcatt tcgttttgcc tattaacgcc tttctattaa gcaaatgcaa
     2641 gcccaccttg cccattggcg caagctactc tcgtttcagt gactttcatt acgtttcttt
     2701 tgtgaatcag atcagaaaac tattatcttt cgttttattt ttatctcacc atgacgcagt
     2761 atcaattgaa acaaaacgaa agatcaatat cgcagtaatc tgaactggag aggaaagtga
     2821 aacatctgac agaaatggtg agacagcaca aagcgggcaa aacaaatgca atttatgccg
     2881 tttgttccgc acatccgctg gtgctggagg ctgcaatccg ctacgccagt gcaaaccaaa
     2941 cgccgttact gattgaagca acctccaatc aggtagacca gttcggcggt tataccggaa
     3001 tgacgcccgc cgattttcgc ggctttgttt gtcagctcgc cgactcgttg aatttcccgc
     3061 aggatgcgct gattctgggt ggtgaccatc tggggccaaa ccgctggcaa aacctgccag
     3121 ccgctcaggc aatggccaat gccgatgatt tgattaaaag ctacgttgcg gcaggattca
     3181 aaaaaatcca ccttgattgc agcatgtcct gtcaggacga tccaattccc ttaactgatg
     3241 acatcgtggc tgaacgcgcc gcccgtctgg cgaaagtggc ggaagaaacc tgtcgcgaac
     3301 actttggcga agccgatctg gagtatgtca ttggtaccga agtgccggta cctggcggcg
     3361 cgcatgaaac cttaagcgag ctggcggtca ccacgccgga tgccgcccgc gccacgctgg
     3421 aagcccatcg tcacgccttt gaaaagcaag gtttgaatgc catctggcca cgcattattg
     3481 ccctggtggt tcaacccggc gtcgaattcg atcacaccaa cgttattgat tatcagcccg
     3541 ccaaagcgag cgccttaagc cagatggtcg aaaactacga aacgctgatt ttcgaagcgc
     3601 actctaccga ttatcaaacg ccgcaatctc tgcgccagct ggtgattgac cactttgcca
     3661 ttctgaaagt tggcccggcg ctgaccttcg ccctgcgtga agcccttttt tctctggcgg
     3721 cgattgaaga agaactggta ccagcgaaag cctgttccgg tctgcgtcag gtgctggaaa
     3781 acgtgatgct cgaccgcccg gaatactggc aaagccacta ccacggtgac ggcaacgcgc
     3841 gtcgtctggc gcgtggttac agctactcag atcgcgtgcg ctattactgg ccggacagcc
     3901 agattgatga cgctttcgct catctggttc gtaatctggc ggattcacca attccgctac
     3961 cgctcatcag ccagtatctg ccgctgcagt acgtgaaagt tcgctccggc gagctgcaac
     4021 cgacgccacg ggaactcatt ataaaccata ttcaggacat cctggcgcag taccacacag
     4081 cctgtgaagg ccaataagca aaacaaagag gaacatgcta tgccaaatat tgttttaagc
     4141 cggattgatg aacgcttgat tcacggtcag gtcggcgttc agtgggtcgg atttgcgggg
     4201 gcaaatctgg tgctggtagc caacgatgag gttgccgaag atccggtaca acaaaacctg
     4261 atggaaatgg tactggcaga agggatcgcc gtacgtttct ggacgctgca aaaagttatc
     4321 gacaacattc atcgcgccgc cgatcgacag aaaatcctgc tggtttgtaa aacacccgcc
     4381 gatttcctga cgctggtgaa aggtggcgtt ccggtgaatc gcattaacgt tggcaatatg
     4441 cactacgcca atggcaaaca acaaatcgcc aaaacggttt ctgtggatgc gggcgatatc
     4501 gcagcattta acgacctgaa agccgctggg gtggaatgct tcgttcaggg cgtcccgaca
     4561 gagcctgctg tggacctctt taaattactt tgagggattc atcatggaaa tcagcctgtt
     4621 gcaggcattt gcgttgggca ttatcgcctt tatcgctggc ctagatatgt ttaacggctt
     4681 aacccatatg caccgcccgg tggtcctcgg cccgttggtc gggctggtac ttggcgatct
     4741 gcataccgga attttaaccg gcgatacgct ggaactagtg tggatggggc tggccccgct
     4801 ggcgggcgca cagccgccta acgtgattat cggtactatc gtcggcacgg cgtttgccat
     4861 tactactggc gtgaaacccg atgtcgcggt cggtgtcgcc gtacctttcg ctgtcgcagt
     4921 acagatgggg attaccttcc tgttctcggt gatgtctggc gtgatgtctc gctgcgaccg
     4981 gatggcggag aacgccgata ctcgcggcat tgaacgcgtg aactatctgg cactgctggc
     5041 actcggcact ttctattttc tctgcgcttt cctgcctatc tacttcggcg cggaacatgc
     5101 aaaaaccatc attgatgtcc tgccgcaacg attaatcgac ggcctcggcg tcgcgggcgg
     5161 catcatgcca gcaatcggtt ttgccgtgct gctgaaaatc atgatgaaaa acgtctacat
     5221 accctacttc atcctgggct ttgttgccgc aacctggctc aagttaccgg tgctggctat
     5281 tgctgccgcc gcgctggcga tggcgctgat cgatctgctg cgtaaatctc ctgaaccgac
     5341 tcaacctgcg gcacagaaag aggaatttga agatggcatc taatcaaacc accctgccga
     5401 acgtcagcga aaatgaagaa acactgctga ctggcgtcaa tgaaaacgtg tatgaagatc
     5461 ggagcattgc cgcggagctg acgaaaaaag atatcaaccg cgtcgcctgg cgttccatgc
     5521 tgttacaagc gtcttttaac tacgaacgta tgcaggcttc tggctggttg tacggtctgc
     5581 tgcctgctct gaaaaagatc cacactaata aacgcgacct ggcgcgcgcc atgaaggggc
     5641 atatgggttt cttcaatacc catccgtttc tggtgacgtt tgttatcggc attatccttg
     5701 cgatggagcg ttctaagcag gacgttaaca gtattcagag caccaaaatt gccgtcggtg
     5761 cgccgcttgg cgggattggc gatgcgatgt tctggctaac gctactgccg atttgtggcg
     5821 ggataggcgc aagcctcgcg ctacaaggct ctattcttgg cgcagtcgtc tttattgtgc
     5881 tgttcaacgt ggtacacttg gggctgcgtt ttggtctggc gcattatgct taccgcatgg
     5941 gcgtggcggc gattcccctt attaaagcga ataccaaaaa agtcggccat gctgcgtcaa
     6001 tcgttggaat gacggtaatc ggcgcactgg tggcaaccta tgttcgttta agcaccacgc
     6061 tggaaatcac ggcgggcgac gcagtagtta agttacaggc tgatgttatc gacaaactga
     6121 tgcctgcctt cttaccgctg gtctacaccc tgaccatgtt ctggctggta cgccgcggct
     6181 agagtccgct gcgcctgatt gcggttaccg tggttctagg gatcgtcggt aaattctgcc
     6241 atttccttta aatacaatga ggtattagat gttaagtatt attttgacag ggcatggcgg
     6301 gtttgccagt ggcatggaaa aagcaatgaa gcaaatcctc ggcgatcaat cgcagtttat
     6361 tgccatcgat tttcccgaaa cctcaagcgc cgcgctgctt acatcgcagc ttgaagaggc
     6421 catcgcccaa ctggattgtg aagacggcat tgtttttctg acggatttgc tgggcggcac
     6481 gccgtttcgc gtagcttcaa cgctggcgat gcaaaaaccg ggctgtgaag tgattaccgg
     6541 caccaatttg caactgctac tggagatggt gctggagcgc gaagggttaa gcggtgaaga
     6601 attccgtgta caggcgctgg agtgtggaca ccgagggctg accagtctgg tggacgagtt
     6661 aggccgctgc catgaagaat gtccggtcga ggaaggaata tgacacacgt tctgcgcgcc
     6721 agaaggctgc tgactgaaga gggatggttc gatgaccatc agttgcgtgt tgctgacggt
     6781 gtcatcgcag caatcgaacc gattccagtg ggcgtgactg aacgcgatgc ggaacggctc
     6841 tgccccgctt acatcgacac ccatgtacac ggtggtgcgg gcgttgatgt aatggatgac
     6901 gcgccggatg tactcgacaa gctggcaatg cacaaggcac gcgaaggtgt cggcagttgg
     6961 ttaccgacca ccgtaaccgc gccgcttaat accattcatg cggcgctgaa acgtattgct
     7021 caacgttgcc aacgcggcgg acctggtgcg caagtgctgg ggagttatct cgaaggaccg
     7081 tacttcacgc cgcagaataa aggcgcgcat ccg
//
LOCUS       DAZPAB010000188         7066 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZPAB010000188 DAZPAB010000000
VERSION     DAZPAB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.188"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..183
                     /gene="ipgA"
                     /locus_tag="ACHUPG_003411"
     CDS             <1..183
                     /gene="ipgA"
                     /locus_tag="ACHUPG_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HFR0862890.1"
                     /translation="NYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLSPDELIESLYEF
                     LFCIKLTIANITSEVN"
     gene            199..825
                     /gene="ipgB1"
                     /locus_tag="ACHUPG_003412"
     CDS             199..825
                     /gene="ipgB1"
                     /locus_tag="ACHUPG_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HFR0862891.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            882..1349
                     /gene="ipgC"
                     /locus_tag="ACHUPG_003413"
     CDS             882..1349
                     /gene="ipgC"
                     /locus_tag="ACHUPG_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HFR0862892.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            1355..3097
                     /gene="ipaB"
                     /locus_tag="ACHUPG_003414"
     CDS             1355..3097
                     /gene="ipaB"
                     /locus_tag="ACHUPG_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HFR0862893.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            3060..4208
                     /gene="ipaC"
                     /locus_tag="ACHUPG_003415"
     CDS             3060..4208
                     /gene="ipaC"
                     /locus_tag="ACHUPG_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HFR0862894.1"
                     /translation="MLQKQFCNKLLLDTNKENVMEIQNTKPTQILYTDISTKQTQSSS
                     ETQKSQNYQQIAAHIPLNVGKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKD
                     LSEMSHTLTPENTLDISSLSSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKS
                     AAENIVRQGLAALSSSITGAVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKE
                     LAGSKLGLNKQIDTNITSPQTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDK
                     IDTQRRTYELNTLSAQQKQNIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASS
                     KQAEEASQVSKEASQATNQLIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            4259..5257
                     /gene="ipaD"
                     /locus_tag="ACHUPG_003416"
     CDS             4259..5257
                     /gene="ipaD"
                     /locus_tag="ACHUPG_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HFR0862895.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            5266..>7066
                     /gene="ipaA"
                     /locus_tag="ACHUPG_003417"
     CDS             5266..>7066
                     /gene="ipaA"
                     /locus_tag="ACHUPG_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HFR0862896.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLK"
BASE COUNT         2626 a         1408 c         1166 g         1866 t
ORIGIN      
        1 aactactctt tgagtagaga ttatggttct tctatctgtc ttctccctga tactcatcaa
       61 tgtgttttga ccaagaaaca ctacaaaaaa tatctttcac ctgatgaact cattgaatct
      121 ttgtatgaat ttttattttg tattaaacta accattgcaa acatcacttc agaagtgaac
      181 taacatatag ggggtatcat gcaaattcta aacaaaatac ttccacaggt tgaattcgcg
      241 attcctcgcc catcatttga ttcgttaagt cgcaataagc ttgtaaagaa aatattatct
      301 gtttttaact taaaacaacg ctttcctcag aagaattttg gatgcccggt gaatataaat
      361 aaaatcaggg acagcgtcat agacaaaata aaagatagta attcggggaa tcaactattt
      421 tgttggatga gccaagagcg aaccacttat gtctcttcaa tgataaacag aagtatagat
      481 gaaatggcca tacacaatgg agttgttttg acctcagata acaaaaaaaa catatttgca
      541 gctattgaaa aaaaattccc ggatataaaa cttgatgaaa aatcagcaca aacatctatc
      601 agtcatacag cactaaacga gattgcctca tctggcctca gagcaaagat tttaaaacgc
      661 tatagcagtg acatggattt gtttaacaca caaatgaagg atcttacaaa tctagtatcc
      721 agttccgtct acgataaaat atttaatgaa tcaacaaaag ttttacaaat agaaataagc
      781 gcagaggttc taaaggctgt ataccgtcaa agcaatacaa attaaatctt ttatattaaa
      841 tcttatactt attaaagtaa agcagacaaa aaggagacct tatgtcttta aatatcaccg
      901 aaaatgaaag catctctact gcagtaattg atgcaattaa ctctggcgct acactgaaag
      961 atattaatgc aattcctgat gatatgatgg atgacattta ttcatatgct tatgactttt
     1021 acaacaaagg aagaatagag gaagctgaag ttttcttcag gtttttatgt atatacgact
     1081 tttacaatgt agactacatt atgggactcg cagctattta tcagataaaa gaacagttcc
     1141 aacaagcagc agacctttat gctgtcgctt ttgcattagg aaaaaatgac tatacaccag
     1201 tattccatac tggacaatgc cagcttcggt tgaaagcccc cttaaaagct aaagagtgct
     1261 tcgaactcgt aattcaacac agcaatgatg aaaaattaaa aataaaagca caatcatact
     1321 tggacgcaat tcaggatatc aaggagtaat tattatgcat aatgtaagca ccacaaccac
     1381 tggttttcct cttgccaaaa tattggcttc cactgagctt ggagacaata ctatccaagc
     1441 tgcaaatgat gcagctaaca aattattttc tcttacaatt gctgatctta ctgctaacca
     1501 aaatattaat acaactaatg cacactcaac ttcaaatata ttaatccctg aacttaaagc
     1561 accaaagtca ttaaatgcaa gttcccaact aacgctttta attggaaacc ttattcaaat
     1621 actcggtgaa aaatctttaa ctgcattaac aaataaaatt actgcttgga agtcccagca
     1681 acaggcaaga cagcaaaaaa acctagaatt ctccgataaa attaacactc ttctatctga
     1741 aactgaagga ctaaccagag actatgaaaa acaaattaat aaactaaaaa acgcagattc
     1801 taaaataaaa gacctagaaa ataaaattaa ccaaattcaa acaagattat ccgaactcga
     1861 cccagagtca ccagaaaaga aaaaattaag ccgggaagaa atacaactca ctatcaaaaa
     1921 agacgcagca gttaaagaca ggacattgat tgagcagaaa accctgtcaa ttcatagcaa
     1981 acttacagat aaatcaatgc aactcgaaaa agaaatagac tctttttctg cattttcaaa
     2041 cacagcatct gctgaacagc tatcaaccca gcagaaatca ttaaccggac ttgccagtgt
     2101 tactcaattg atggcaacct ttattcaact agttggaaaa aataatgaag aatctttaaa
     2161 aaatgatctg gctctattcc agtctctcca agaatcaaga aaaactgaaa tggagagaaa
     2221 atctgatgag tatgctgctg aagtacgtaa agcagaagaa ctcaacagag taatgggttg
     2281 tgttgggaaa atacttgggg cacttttaac tatcgttagt gttgttgcag cagctttttc
     2341 tggaggagcc tctctagcac tggcagctgt tggtttagct cttatggtta cggatgctat
     2401 agtacaagca gcgaccggca attccttcat ggaacaagcc ctgaatccga tcatgaaagc
     2461 agtcattgaa cccttaatca aactcctttc agatgcattt acaaaaatgc tcgaaggctt
     2521 gggcgtcgac tcgaaaaaag ccaaaatgat tggctctatt ctgggggcaa tcgcaggcgc
     2581 tcttgtccta gttgcagcag tcgttctcgt agccactgtt ggtaaacagg cagcagcaaa
     2641 acttgcagaa aatattggca aaataatagg taaaaccctc acagacctta taccaaagtt
     2701 tctcaagaat ttttcttctc aactggacga tttaatcact aatgctgttg ccagattaaa
     2761 taaatttctt ggtgcagcgg gtgatgaagt aatatccaaa caaattattt ccacccattt
     2821 aaaccaagca gttttattag gagaaagtgt taactctgcc acacaagcgg gaggaagtgt
     2881 cgcttctgct gttttccaga acagcgcgtc gacaaatcta gcagacctga cattatcgaa
     2941 atatcaagtt gaacaactgt caaaatatat cagtgaagca atagaaaaat tcggccaatt
     3001 gcaggaagta attgcagatc tattagcctc aatgtccaac tctcaggcta atagaactga
     3061 tgttgcaaaa gcaattttgc aacaaactac tgcttgatac aaataaggag aatgttatgg
     3121 aaattcaaaa cacaaaacca acccagattt tatatacaga tatatccaca aaacaaactc
     3181 aaagttcttc cgaaacacaa aaatcacaaa attatcagca gattgcagcg catattccac
     3241 ttaatgtcgg taaaaatccc gtattaacaa ccacattaaa tgatgatcaa cttttaaagt
     3301 tatcagagca ggttcagcat gattcagaaa tcattgctcg ccttactgac aaaaagatga
     3361 aagatctttc agagatgagt cacaccctta ctccagagaa cactctggat atttccagtc
     3421 tttcttctaa tgctgtttct ttaattatta gtgtagccgt tctactttct gctctccgca
     3481 ctgcagaaac taaattgggc tctcaattgt cattgattgc gttcgatgct acaaaatcag
     3541 ctgcagagaa cattgttcgg caaggcctgg cagccctatc atcaagcatt actggagcag
     3601 tcacacaagt aggtataacg ggtatcggtg ccaaaaaaac gcattcaggg attagcgacc
     3661 aaaaaggagc cttaagaaag aaccttgcca ctgctcaatc tcttgaaaaa gagcttgcag
     3721 gttctaaatt agggttaaat aaacaaatag atacaaatat cacctcacca caaactaact
     3781 ctagcacaaa atttttaggt aaaaataaac tggcgccaga taatatatcc ctgtcaactg
     3841 aacataaaac ttctcttagt tctcccgata tttctttgca ggataaaatt gacacccaga
     3901 gaagaactta cgagctcaat accctttctg cgcagcaaaa acaaaacatt ggccgtgcaa
     3961 caatggaaac atcagccgtt gctggtaata tatccacatc aggagggcgt tatgcatctg
     4021 ctcttgaaga agaagaacaa ctaatcagtc aggccagcag taaacaagca gaggaagcat
     4081 cccaagtatc taaagaagca tcccaagcga caaatcaatt aatacaaaaa ttattgaata
     4141 taattgacag catcaaccaa tcaaagaatt cgacagccag tcagattgct ggtaacattc
     4201 gagcttaata tatccaagag ccataataat atatggctct tcctgtaagg aaataaccat
     4261 gaatataaca actctgacta atagtatttc cacctcatca ttcagtccaa acaataccaa
     4321 cggttcatca accgaaacag ttaattctga tataaaaaca acgaccagtt ctcatcctgt
     4381 aagttccctt actatgctca acgacaccct tcataatatc agaacaacaa atcaggcatt
     4441 aaagaaagag ctttcacaaa aaacgttgac taaaacatcg ctagaagaaa tagcattaca
     4501 ttcatctcag attagcatgg atgtaaataa atccgctcaa ctattagata ttctttccag
     4561 gcacgaatat ccaattaata aagacgcaag agaattatta cattcagctc cgaaagaagc
     4621 cgagcttgat ggagatcaaa tgatatctca tagagaactg tgggctaaaa ttgcaaactc
     4681 catcaatgat attaatgaac agtatctgaa agtatatgaa catgccgtta gttcatatac
     4741 tcaaatgtat caagatttta gcgctgttct ttccagtctt gccggctgga tctctcccgg
     4801 aggtaacgac ggaaactccg tgaaattaca agtcaactcg cttaaaaagg cattggaaga
     4861 actcaaggaa aaatataaag ataaaccgct atatccagca aataatactg ttagtcagga
     4921 acaagcaaat aaatggctta cagaattagg tggaacaatc ggcaaggtat ctcaaaaaaa
     4981 cgggggatat gttgtcagta taaacatgac cccaatagac aatatgttaa aaagcttaga
     5041 taatctaggt ggaaatggcg aggttgtgct agataatgca aaatatcagg catggaatgc
     5101 cggattctct gccgaagatg aaacaatgaa aaataatctt caaactttag ttcaaaaata
     5161 cagtaatgcc aatagtattt ttgataattt agtaaaggtt ttgagtagta caataagctc
     5221 atgtacagat acagataaac tttttctcca tttctgaggt gcgccatgca taatgtaaat
     5281 aatactcaag cgccaacatt cttatataag gcaacttcac catcatcaac agaatacagc
     5341 gagttaaaaa gcaaaatatc cgatatccat agttcgcaaa cttctctaaa aacaccagca
     5401 tcagtgtctg aaaaagaaaa ctttgcaacg tcttttaatc agaaatgtct tgatttttta
     5461 ttttcttcct cagggaaaga agatgtgtta agaagcattt attccaactc aatgaatgcg
     5521 tatgccaaaa gcgagattct cgaattttca aatgttttgt actccttagt acatcaaaat
     5581 ggtcttaatt ttgaaaacga aaagggactt caaaaaattg tcgcacagta ttcggaacta
     5641 attataaaag ataaattatc ccaagattct gcctttggac catggtcggc aaagaataag
     5701 aaactccatc aattacgaca aaacattgag cacagacttg cactattagc acaacaacac
     5761 acatctggtg aagctttatc attgggacaa aaactcctca atactgaagt atcatcattt
     5821 atcaagaata atattcttgc tgaattaaag ttaagtaatg aaactgtttc atctctcaaa
     5881 ctagatgatt tagttgacgc acaggcaaaa cttgcctttg atagtttgcg caatcaacgt
     5941 aaaaatacta ttgatagtaa aggatttggt ataggtaaac tgtcaagaga cttaaataca
     6001 gtagccgtgt ttcctgagct gttgagaaaa gtccttaatg atattttaga agatataaaa
     6061 gattcgcatc ctatccaaga tggcctccct acacctcccg aagatatgcc agatggcgga
     6121 ccaacccccg gagccaatga gaaaacatcc caacctgtaa ttcactatca tataaataat
     6181 gataatagaa cttacgataa tagagttttt gacaacagag tatatgacaa tagctatcac
     6241 gagaacccag aaaatgatgc acagtctcct acttctcaga caaacgatct attatcccgt
     6301 aacggaaact cattactaaa tccacaaaga gcactagttc aaaaagtaac ttccgttcta
     6361 ccacactcta tatcagatac tgtccagaca tttgcaaata attcagcttt agaaaaggct
     6421 ttcaaccata ctccagataa ttcggatgga ataggttcag acctgttaac tacgagtagt
     6481 caagaaagat ctgcaaataa ctctctttct cggggacaca ggcctctgaa catacagaac
     6541 tcttcaacca ccccccctct ccacccggaa ggagtgacaa gcagtaatga taactcatca
     6601 gatacaacta aaagtagcgc ttctctttct catagagtag cttcgcaaat caataaattc
     6661 aactcaaaca ctgattcaaa agtacttcag actgattttt tatcaagaaa tggagacaca
     6721 tatttaacac gggaaacgat atttgaagct tcaaaaaaag taacaaactc cctaagtaat
     6781 cttatatctc tcattggaac taaatcagga acacaagaac gagagttaca ggaaaaatca
     6841 aaggacatta caaaatccac aacagaacat agaataaaca acaaattaaa agttacagat
     6901 gcaaatataa gaaactacgt aacagaaacc aacgcagata caattgataa aaatcatgcg
     6961 atctatgaaa aggcaaaaga agtatctagc gccctcagca aggtattgtc aaaaattgac
     7021 gatacctctg cagaattact tacagatgat atatctgatt taaaaa
//
LOCUS       DAZPAB010000189         7031 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZPAB010000189 DAZPAB010000000
VERSION     DAZPAB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.189"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1..3052
                     /locus_tag="ACHUPG_003418"
                     /pseudo
     CDS             1..3052
                     /locus_tag="ACHUPG_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3062..6708
                     /locus_tag="ACHUPG_003419"
                     /pseudo
     CDS             3062..6708
                     /locus_tag="ACHUPG_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6769..>7031
                     /gene="yehK"
                     /locus_tag="ACHUPG_003420"
     CDS             6769..>7031
                     /gene="yehK"
                     /locus_tag="ACHUPG_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFR0862897.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPV"
BASE COUNT         1846 a         1822 c         1710 g         1653 t
ORIGIN      
        1 tgggattatg cgaataatgt cattattatt ctgccgtctg gcattgcacc tgactacttg
       61 gagcaatatt ccagatttga attacgccta cgtaaacatt tatcactggc ggaagagtct
      121 ctctggcaaa aatgtgcaca aaaacttatt gccgcaattc cacatattcc agaatggcgg
      181 caaccattaa ttgctttgtt attacccgaa aaaccagaaa ttgcacatga aattgcccag
      241 catctactgg ggcaaaaaaa attaccctcg cttgagtggt taaaaatagt ggcgactgat
      301 gagcacattc ttgcctcatt agaaaaatat cacgagccat atgccatttt tgatgattac
      361 tattgtggtg cgatatggtc agccaccgta ttacaggagc aaggtgttgc agccctgccc
      421 cgatttgctc cctatgccgc aagtgactac tgcgccgatg tgttgcgtca tatcaatcat
      481 ccgttcgcat taacactgct tatacgtgta gccgggcata ctaaacgctg tcacgatcga
      541 atgacgaaag cctgcgctgc gttcccacac gcagcactgg cagcactggc ggaacttcta
      601 gtgcaaaaag aagagaatag ttggcgcatt atgctaatga cgatgtttat ctcgcaacca
      661 gcactggcag aacaggtcat tccctggctt tcgacacccg cagttgcagt gctgaaatca
      721 tgccagcacc aactgacaca gccctcaaac catgccagcg ccgatctact gccagccata
      781 gtagtctccc caccatggat ttcgaaaaag aaaaaatcgc cgattccggt gctggattta
      841 gcgccattag gcatcgagcc aatctgttat ctgacagaag aaatcagtaa tcaacttttg
      901 gcgaaatata tctggtattc aaaacacatc acggttagcc atgaagaaag tactaccaac
      961 ctgttggcaa ggatgggttt tcaacgacgg atcgctggta catatattaa agctcccgaa
     1021 gcggtagttg aggcatggct aaatgaagat tattcaacct tactaagtga atttaaggtg
     1081 tttcattcac ctaccgggca ttattggcag ttggggattt tgacaacatt gccgctggag
     1141 gctggagaaa gcagtaaaag catggaatgc ccttactcta tctccacata ccgataccga
     1201 atacgccatg ttacattttg gactcaaagg tttacctggt ttagttaact caattacacg
     1261 ctatccacag gaagccttgc ccatcacgaa ttacttcgca gcgagtgagc tggctcctgc
     1321 cgtcgcccgt gccttcaaca aactcaaaac gctacgcgaa aacgcccgca cctggctgtt
     1381 gaaatacccg gaacatgccc ttaccggcct gctgcctgcg gcgctcggca aagccggtga
     1441 agcacaggat aacgcccgcg ctgccttgcg tatgcttacc gaaaacggtc atcagccatt
     1501 actgcaagaa atcgcccggc gttataacca gtcggaagta acagacgcgg tgaacgctct
     1561 gcttgcgctc gatcccttag ataatcaccc gacaaaaatc cccactcttc cgacctttta
     1621 tcagccatcg atctggacgc gcccggtatt aaaagcaaac gcccaatcac tgccagataa
     1681 cgccctcctc cacctcggcg aaatgctccg cttccctcag gaagaggctc tgtatccggg
     1741 attattgcag gtgaaagacg cctgtaccgc tgactctctg gctgaatttg cctgggatct
     1801 gtttaccgcc tggcagaccg ctggcgcgcc gtcgaaagag agttgggcgt tcactgcgtt
     1861 aggcgttctc ggtaacgatg acaccgcccg caaactgacg ccattaatac gcgcctggcc
     1921 tggtgaatcc cagcataaac gcgccaccgt tgggttggat attctcgccg ctatcggtag
     1981 tgatatcgcc ctgatgcagc ttaacggcat tgctcagaaa ctgaaattca aagcattaca
     2041 ggagcgggca aaagaaaaga ttgccgacat tgccgagagc cgcgaactca cggtagcgga
     2101 gcttgaagat cggttagcac cggatctcgg tctggatgat aacggttcgt tgctgctgga
     2161 ttttggccca cgccagttca ccgtcagctt tgatgaaacc ttaaaaccgt ttgtgcgtga
     2221 tgtttccggc agccgcctga aagacctgcc aaaaccaaac aaaagcgatt atgagtcaca
     2281 ggcgaacgat gctgttaacc gctacaaatt gctgaaaaaa gatgcgcgta ccgtcgcggc
     2341 ccagcaggta gcaaggcttg aatccgccat gtgcctgcgt cgccgctggt cgccggaaaa
     2401 cttccaactc ttcctggttg tgcatccgct ggttcgccac ttaacccgcc gtctgatttg
     2461 gggcgtttat agcgccgaca accagctact ggcctgtttc cgggtagcgg aagataacag
     2521 ctacagcacc gctgacgatg atcttttcac cctgccggaa ggcgatatct ctatcggcat
     2581 tcctcacgtt ctggaaatat caccgacgga tgctgtcgcc tttggtcagc tttttgccga
     2641 ctacgaactg ctaccaccgt tccgtcagct tgaccgtaac agttacgccc tgacagaagc
     2701 cgagcgcaac gccagcgaac tgacccgctg ggcaggcaga aaatgcccga gcggccgggt
     2761 catggggctg gcaaataaag gctggataaa gggcgaacca caggatggag gctggatgat
     2821 caaacctctc ggtcgctggt cgttaatcat ggaaatcgac gaaggttttg cggtgggtat
     2881 atcaccagcc gaacttagcg ctgagcagct cttaagcaag ctgtggctat gggaaggcaa
     2941 agcggaaagc tatggctggg ggagtaattc aacacaggaa gcgcagttct ccgtactcga
     3001 tgccatcacc gccagcgagc taattaacga tattgaagcc ctgtttgaat aaggaaagag
     3061 catggacaag gaattaccgt ggctggcgga taacgcccaa ctggaactga aatataaaaa
     3121 aggcaaaaca ccactcagtc atcgcaattg gccgggcgaa ccagtgcctg ttatcactga
     3181 aagcatcatt cagacactgg gtgatgaatt gctacaaaaa gctgagaaga aaaaaaacat
     3241 tgtctggcgt tatgaaaatt tttcaccgga gtggcagtcc gccattacgc aggccatcaa
     3301 cttgatcggc gaacacaaac cctcagtccc ggcccgcaca atggcggcac tagcctttat
     3361 cgcgcaaaat gacaaccaac agttgctcga cgaaatcgtc caacaagagg ggctggaata
     3421 tgcgactgag gtggtgattg cacgccagtt tattgcacgg tgttatgaga gtgatcctct
     3481 ggtagtcaca ttgcagtatc agaacgagga ctatggctat ggttatcgct cagaaaccta
     3541 taacgaattc gatctccgac tgcgtaaaca cctctctctg gcagaggaaa gctgctggca
     3601 gcgttgcgcc gacaaactca ttgccgcact gccaggaata accaaagttc gccgcccttt
     3661 tattgcgctg atcctccccg aaaaaccaga aatcgccaat gagttggtaa gccttgaatg
     3721 cccgcggact cattttcatt ctaaggagtg gttaaaagtt gttgctactg accccaaagc
     3781 ggtgagaaaa ctcgaacgct actggagcca ggatatattt agcgatcgag aagccagcta
     3841 catgtcgcat gaaaaccact tcggctacgc ggcctgcgct gcccttttgc gcgaacaagg
     3901 actggcagtc gttccgcgcc tcgcgatata tgcccataaa gaagattgcg gcagtctgct
     3961 ggtacaaatt aaccatccgc aagtcatccg caccttgctg ttagttgctg ataaaaacaa
     4021 acccagcctg caacgtgtgg ctaaatacag taaaaacttc ccccatgcga cactcgccgc
     4081 actggcagaa ctgctggcgt taaaagaacc accagcccgc ccaggttatc caatcatcga
     4141 agacaaaaag ctgcctgcac agcaaaaagc tcgagatgaa tactggcgta cgctgttaca
     4201 aacgctgatg gcatcgcagc cacaactggc agcagaagtg atgccgtggt taagtactca
     4261 agcccgggca gtgctgaata gttatttatc ggcaccgccc aaaccagtta ttgatagcac
     4321 cgataacagc caaatgcctg aaatcctcgt ttcaccacca tggcgtggga gaaagaaaaa
     4381 gacttccata cgtcactttt cgctgcctga tcttacgctt gcaccgcaaa cgtacaggtc
     4441 gccgaataaa cactggacgc cggaacagca aactgcaatt gaccatttta gcgccatgtc
     4501 ttttgacgaa cgtcttgcta cgagaggagc ggatatcttc ttgcgggaat taggttttga
     4561 cgttcatagc ctggaattta ataagcgtct tcttaatgga cagttggatg cctttgatgc
     4621 tcagcaactg caagaataca catcaatagg actattgcgt acttatcacg cgcatcaggc
     4681 caaatctaaa cagtaccggg aagacgccac caatgcatta ctggcacaag atagcattgc
     4741 gttgatggga gggtggcgaa cttttcagca cggattttac tatggtaatg aacagcgttg
     4801 ggggatttgg aatctgtacc ttatcgcgca gatgccgcgc gaaatggcgg tatcgtgctg
     4861 gcaacaaatt gtcgaggctg attttcatta caccggggtg gaatatttgt taagtgtgct
     4921 gggtaccgat gctttgcctg gattaaacac cgcctttgcc cgacatccaa aagagatatt
     4981 tccgctgctg attcatttcg gcgcaacaga actggccctg cccattgccc gcgtctggca
     5041 cctttttgcg ggccagcgta atctggctcg ccagtggatt ttacaatggc cggaacatac
     5101 ggctactgca cttatccctc ttgtcttcac caaatctagc gataaaagcg aagctgcatt
     5161 acttgccctg cgtttacttt acgaacatgg gcatggcgaa tggctacaaa ctgtagccaa
     5221 tcgttggcag cgtacagatc tatggcctgc cctggaacaa ttacttaaac agagtccaat
     5281 agagatttac ccggcacgca ttccaaaagc ccctgatttc tggcatccgc aaatgtggtc
     5341 caggccgcgc cttatcacta ataatcaacc tgttaccgat gacgctctgg aaattatcgg
     5401 cgaaatgctg cgctttaccc aggggggacg tttttatagc gggctggaac aactgaaaac
     5461 gttctgccag ccacaaacgc tggcagcttt tgcctgggat ctcttcactg cgtggcaaca
     5521 agctggtgcc cccgcaaaag acaactgggc atttctggcg ttaagtctct ttggtgacga
     5581 aagcacggca cgggatctaa cgacacatat cctcgcctgg ccacaagaag gcaaatctgc
     5641 ccgtgcggtc agtggcatga acatccttac cctgatgaat aatgatatgg cgctgatgca
     5701 gctgcatcat atatcgcaac gggcgaaatc ccgcccctta cgtgataacg cggcggaatt
     5761 tcttcaggta gtcgcagaaa atcgcgggct aagccaggaa gagttagcgg acagattagt
     5821 cccaacactg ggccttaatg atccgcaggc gttgagtttt gattttggtc cccggcagtt
     5881 taccgttcgc ttcgatgaaa acctcaaccc ggttatcttt gatcagcaaa acgttcgcca
     5941 gaaaagtgtt ccccggttgc gcgccgatga cgatcaactg aaagcgcccg aggcactggc
     6001 ccgactaaaa gggttaaaaa aagatgctac tcaggtgagc aaaaacctgc tcccgcgtct
     6061 tgaagctgcc ctacgtacca cccgacgctg gtcgctggca gattttcatt ctctgtttgt
     6121 taatcatccc tttacccgtc tggttaccca gcgattaata tggggggtta tccggcaaat
     6181 gaaccgcgtc gtttactcaa cgcctttcgt gtggccgcag agggggagtt ctgcaatgcg
     6241 caagatgagc caattgacct gcctgcggac gctctgattg gcattgccca cccgttagaa
     6301 atgacagcag aaatgcgcag tgaatttgca cagctttttg ccgattacga aattatgccg
     6361 ccttttcgcc agttgtcgcg ccgcacggtg ctgctcacac ctgacgagtc aaccagtaac
     6421 agcctgactc gctgggaagg taaatccgct accgttgggc aacttatggg aatgcgatac
     6481 aaaggctggg agtcaggtta tgaggacgca tttgtctatg acctgggtga gtaccggctg
     6541 gtccttaagt tttcacccgg ttttaaccac tacaatgttg atagcaaagc gctaatgagc
     6601 ttccgttctc ttcgagtgta ccgtgacaat aaatccgtca cttttgccga acttgatgtg
     6661 tttgatttga gtgaggcgtt aagcgcacct gacgtcattt tccattaaaa caaagcgggc
     6721 agtgatgcga ctgcccgtta tctacacgac ttaccagcgg ggaaagcgat gatcgtgcaa
     6781 aaagagctgg ttgctattta cgattatgag gtccctgtac ctgaagatcc gttttccttc
     6841 agacttgaga tccataaatg ctctgaatta tttacaggtt ccgtctaccg actggaacgg
     6901 ttccggctac gtccaacatt tcatcaacgt gatcgagagg atgttgaccc gctaataaat
     6961 gatgcgttga tttatataag agatgagttt attgatgagc aaaaattacg aggcgaatca
     7021 cctgaacctg t
//
LOCUS       DAZPAB010000190         7000 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZPAB010000190 DAZPAB010000000
VERSION     DAZPAB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.190"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            203..1216
                     /gene="intI1"
                     /locus_tag="ACHUPG_003421"
     CDS             203..1216
                     /gene="intI1"
                     /locus_tag="ACHUPG_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR0862898.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            1419..1769
                     /locus_tag="ACHUPG_003422"
                     /pseudo
     CDS             1419..1769
                     /locus_tag="ACHUPG_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            1895..2455
                     /locus_tag="ACHUPG_003423"
     CDS             1895..2455
                     /locus_tag="ACHUPG_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0862899.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            2458..5424
                     /locus_tag="ACHUPG_003424"
     CDS             2458..5424
                     /locus_tag="ACHUPG_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFR0862900.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            5491..5868
                     /locus_tag="ACHUPG_003425"
     CDS             5491..5868
                     /locus_tag="ACHUPG_003425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0862901.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(6069..6728)
                     /locus_tag="ACHUPG_003426"
     CDS             complement(6069..6728)
                     /locus_tag="ACHUPG_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFR0862902.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1515 a         2083 c         1963 g         1439 t
ORIGIN      
        1 attggtttgg taaagggctt aattttaacg gctaacaatt aatgaggctc cgggttcgcc
       61 caactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
      121 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
      181 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
      241 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
      301 cgaacaggct tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
      361 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
      421 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt
      481 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
      541 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
      601 tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg atcagtgagg gtttgcaact
      661 gcgggtcaag gatctggatt tcgatcacgg cacgatcatc gtgcgggagg gcaagggctc
      721 caaggatcgg gccttgatgt tacccgagag cttggcaccc agcctgcgcg agcagctgtc
      781 gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc cgcagcggcg ttgcgcttcc
      841 cgacgccctt gagcggaagt atccgcgcgc cgggcattcc tggccgtggt tctgggtttt
      901 tgcgcagcac acgcattcga ccgatccacg gagcggtgtc gtgcgtcgcc atcacatgta
      961 tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa caagcaggca tcacgaagcc
     1021 cgccacaccg cacaccctcc gccactcgtt cgcgacggcc ttgctccgca gcggttacga
     1081 cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc tctacgacga tgatttacac
     1141 gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg cttgatgcgc tgccgcccct
     1201 cactagtgag aggtagggca gcgcaagtca atcctggcgg attcactacc cctgcgcgaa
     1261 ggccatcggt gccgcatcga acggccggtt gcggaaagtc ctccctgcgt ccgctgatgg
     1321 ccggcagcag cccgtcgttg cctgatggat ccaacccctc cgctgctata gtgcagtcgg
     1381 cttctgacgt tcagtgcagc cgtcttctga aaacgacaat ggaggtggta gccgagggtg
     1441 tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg ttgcgacacg gtgcagggtt
     1501 tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg cttcgtcaac caatggagga
     1561 acaccaccat gaacgccaat gaaccgagca ccagttgctg cgtgtgctgc aaggaaatcc
     1621 cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt ggagcatttc tgcgggctgg
     1681 agtgctatca gcgcttccag gcgcgggcca gcactgcgac cgaaaccagc gtcaaaccgg
     1741 acgcttgtga ttcgccgccg tcaggttgag gcatacccta acctgatgtc agatgccatg
     1801 tgtaaattgc gtcaggatag gattgaattt tgaatttatt gacatatctc gttgaaggtc
     1861 atagagtctt ccctgacatt ttgcagggaa ttccatgact ggacagcgca ttgggtatat
     1921 cagggtcagc accttcgacc agaacccgga acggcaactg gaaggcgtca aggttgatcg
     1981 cgcttttagc gacaaggcat ccggcaagga tgtcaagcgt ccgcaactgg aagcgctgat
     2041 aagcttcgcc cgcaccggcg acaccgtggt ggtgcatagc atggatcgcc tggcgcgcaa
     2101 tctcgatgat ttgcgccgga tcgtgcaaac gctgacacaa cgcggcgtgc atatcgaatt
     2161 cgtcaaggaa cacctcagtt ttactggcga agactctccg atggcgaacc tgatgctctc
     2221 ggtgatgggc gcgttcgccg agttcgagcg cgccctgatc cgcgagcgtc agcgcgaggg
     2281 tattgcgctc gccaagcaac gcggggctta ccgtggcagg aagaaatccc tgtcgtctga
     2341 gcgtattgcc gaactgcgcc aacgtgtcga ggctggcgag caaaagacca agcttgctcg
     2401 tgaattcgga atcagtcgcg aaaccctgta tcaatacttg agaacggatc agtaaatatg
     2461 ccacgtcgtt ccatcctgtc cgccgccgag cgggaaagcc tgctggcgtt gccggactcc
     2521 aaggacgacc tgatccgaca ttacacattc aacgataccg acctctcgat catccgacag
     2581 cggcgcgggc cagccaatcg gctgggcttc gcggtgcagc tctgttacct gcgctttccc
     2641 ggcgtcatcc tgggcgtcga tgaactaccg ttcccgccct tgttgaagct ggtcgccgac
     2701 cagctcaagg tcggcgtcga aagctggaac gagtacggcc agcgggagca gacccggcgc
     2761 gagcacctga gcgagctgca aaccgtgttc ggtttccggc ccttcaccat gagccattac
     2821 cggcaggccg tccagatgct gaccgagctg gcgatgcaaa ccgacaaagg catcgtgctg
     2881 gccagcgcct tgatcgggca cctgcggcgg cagtcggtca ttctgcccgc cctcaacgcc
     2941 gtcgagcggg cgagtgccga ggcgatcacc cgtgctaacc ggcgcatcta cgacgccttg
     3001 gccgaaccac tggcggacgc gcatcgccgc cgcctcgacg atctgctcaa gcgccgggac
     3061 aacggcaaga cgacctggtt ggcttggttg cgccagtctc cggccaagcc aaattcgcgg
     3121 catatgctgg aacacatcga acgcctcaag gcatggcagg cactcgatct gcctaccggc
     3181 atcgagcggc tggttcacca gaaccgcctg ctcaagattg cccgcgaggg cggccagatg
     3241 acacccgccg acctggccaa attcgagccg caacggcgct acgccactct cgtggcgctg
     3301 gccaccgagg gcatggccac cgtcaccgac gaaatcatcg acctgcacga ccgcatcctg
     3361 ggtaagctgt ttaacgctgc caagaataag catcagcagc agttccaggc gtcaggcaag
     3421 gccatcaacg ccaaggtacg tctgtacggg cgcatcggtc aggcgctgat cgacgccaag
     3481 caatcaggcc gcgatgcgtt tgccgccatc gaggccgtca tgtcctggga ttcctttgcc
     3541 gagagcgtca ccgaggcgca gaagctcgcg caacccgatg acttcgattt cctgcatcgc
     3601 atcggcgaga gctacgccac cctgcgccgc tatgcaccgg aattccttgc cgtgctcaag
     3661 ctgcgggccg cgcccgccgc caaaaacgtg cttgatgcca ttgaggtgct gcgcggcatg
     3721 aacaccgaca acgcccgcaa gctgccagcc gatgcaccga ccggcttcat caagccgcgc
     3781 tggcagaaac tggtgatgac cgacgccggc atcgaccggc gctactacga actgtgcgcg
     3841 ctgtccgagt tgaagaactc cctgcgctcg ggcgacatct gggtgcaggg ttcacgccag
     3901 ttcaaggact tcgaggacta cctggtaccg cccgagaagt tcaccagcct caagcagtcc
     3961 agcgaattgc cgctggccgt ggccaccgac tgcgaacaat atctgcatga gcggctgacg
     4021 ctgctggaag cacaacttgc caccgtcaac cgcatggcgg cagccaacga cctgccggat
     4081 gccatcatca ccgagtcggg cttgaagatc acgccgctgg atgcggcggt gcccgacacc
     4141 gcgcaggcgc tgatagacca gacagccatg gtcctgccgc acgtcaagat caccgaactg
     4201 ctgctcgaag tcgatgagtg gacgggcttc acccggcact tcacgcactt gaaatcgggc
     4261 gatctggcca aggacaagaa cctgttgttg accacgatcc tggccgacgc gatcaacctg
     4321 ggcctgacca agatggccga gtcctgcccc ggcacgacct acgcgaagct cgcttggctg
     4381 caagcctggc atacccgcga cgaaacgtac tcgacagcgt tggctgaact ggtcaacgct
     4441 cagtttcggc atccctttgc cgggcactgg ggcgatggca ccacatcatc atcggacgga
     4501 cagaatttcc gaaccgctag caaggcaaag agcacggggc acatcaaccc aaaatatggc
     4561 agcagcccag gacggacttt ctacacccac atctccgacc aatacgcgcc attccacacc
     4621 aaggtggtca atgtcggcct gcgcgactca acctacgtgc tcgacggcct gctgtaccac
     4681 gaatccgacc tgcggatcga ggagcactac accgacacgg cgggcttcac cgatcacgtc
     4741 ttcgccctga tgcacctctt gggcttccgc ttcgcgccgc gcatccgcga cctgggcgac
     4801 accaagctct acatcccgaa gggcgatgcc gcctatgacg cgctcaagcc gatgatcggc
     4861 ggcacgctca acatcaagca cgtccgcgcc cattgggacg aaatcctgcg gctggccacc
     4921 tcgatcaagc agggcacggt gacggcctcg ctgatgctca ggaaactcgg cagctacccg
     4981 cgccagaacg gcttggccgt cgcgctgcgc gagttgggcc gcatcgagcg cacgctgttc
     5041 atcctcgact ggctgcaaag cgtcgagcta cgccgccgcg tgcatgccgg gctgaacaag
     5101 ggcgaggcgc gcaatgcgct ggcccgtgcc gtgttcttca accgccttgg tgaaatccgt
     5161 gaccgcagtt tcgagcagca gcgctaccgg gccagcggcc tcaacctggt gacggcggcc
     5221 atcgtgctgt ggaacacggt ctacctggag cgtgcggcgc atgcgttgcg cggcaatggt
     5281 catgccgtcg atgactcgct attgcagtac ctgtcgccac tcggctggga gcacatcaac
     5341 ctgaccggtg attacctatg gcgcagcagc gccaagatcg gcgcggggaa gttcaggccg
     5401 ctacggcctc tgcaaccggc ttagcgtgct ttattttccg ttttctgaga cgacccctat
     5461 tatggcgaag gtatcattca ggaagcgttg atgaaggatt atctctgtaa aaagttattc
     5521 aaccggcttt ccggtaccct ggtgatcaga gcgcgctgcg gcaataacat tactggcctg
     5581 gcatgttgca atattcttta tccctcgccc cgatacagcg gtcagctgca tattaaagag
     5641 ctgtatgttt ctcagtgcga tcgaaataag ggcacgggta aagcgataat gcgctttata
     5701 gcccggcttg cgcttgaaca ggaatgcctt agccttagct ggaacgctga aaaatccaac
     5761 cccggcgcta accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat
     5821 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
     5881 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     5941 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     6001 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
     6061 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     6121 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     6181 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     6241 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     6301 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     6361 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     6421 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     6481 accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca
     6541 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     6601 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     6661 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     6721 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     6781 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     6841 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat
     6901 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct
     6961 gccaacttac tgatttagtg tatgatggtg tttttgaggt
//
LOCUS       DAZPAB010000191         6791 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZPAB010000191 DAZPAB010000000
VERSION     DAZPAB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.191"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(195..680)
                     /gene="sixA"
                     /locus_tag="ACHUPG_003427"
     CDS             complement(195..680)
                     /gene="sixA"
                     /locus_tag="ACHUPG_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR0862903.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(883..3027)
                     /gene="fadJ"
                     /locus_tag="ACHUPG_003428"
     CDS             complement(883..3027)
                     /gene="fadJ"
                     /locus_tag="ACHUPG_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR0862904.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3027..4337)
                     /gene="fadI"
                     /locus_tag="ACHUPG_003429"
     CDS             complement(3027..4337)
                     /gene="fadI"
                     /locus_tag="ACHUPG_003429"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR0862905.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4517..4801)
                     /locus_tag="ACHUPG_003430"
     CDS             complement(4517..4801)
                     /locus_tag="ACHUPG_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR0862906.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5173..6513
                     /gene="fadL"
                     /locus_tag="ACHUPG_003431"
     CDS             5173..6513
                     /gene="fadL"
                     /locus_tag="ACHUPG_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR0862907.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1691 a         1818 c         1613 g         1669 t
ORIGIN      
        1 atattattca tttcaacatg taactttggg caaaatcaag atacgcgtag aagaataatt
       61 tccgaatgaa tgtagaaagt gttttattta tagataagga gagtcttcga tagtagtgag
      121 gttgacagat atccgctgcc taatgagcaa ttatgcccaa tgaatctacc tcattgggca
      181 taatttggaa cacatcagat agcttttgcc atcttcagat tacacgggct catctgccag
      241 ttgaatgtac cgtttccgct ttcatcaagc gttacgctgg caatggccga ggtggtaaac
      301 ataggcggcg tttcgcccgg acataactcg gcaaccagat acccgactaa cggtaagtga
      361 gagatcacca gtaccgaagc aacaccttca ttggtcaacg cctgcaaata ggcactgacc
      421 agaccaacat cgccgcaggg cgttaactcc ggcagaactt ctgcactgga aggcaggttc
      481 agacaatccc ctacttcttc cagtgtttgc tcggctcgca ggaacgggct caccagaacg
      541 cgttcgattt ccactttttg acctttcagc cagttcgcca tcaggcgaga ttcgtcacaa
      601 ccattagtgg tcagaggacg aaccgaatca ctggcggaat cgagggctgc gtcgccgtga
      661 cgcatgataa aaacttgcat attgcaccgc ttttgttaac cagattcgcc cgttttcttt
      721 catctgaaac cagagaaaga aaacggtggc cggcattgtg ccttatccat tcaccgaatg
      781 aaacgctgtt ttttacctca atggcgtaag tatagtcaat atgcgtttac attttgacca
      841 acactccgcc attcagcgcg gattcatata gctttgacct tcttattgca ggtcagttgc
      901 agttgttttc caaaaacttt ccccacgcgc gcccatctcg accaaacgct cgcaaggggt
      961 aaaacgggaa ccatactgcg tggccagtcg ttgcattatt gcaaccactt cgcccgcgcc
     1021 gagagaatcg atatagcgga acggtccacc gagaaatggc ggaaaaccaa tgccaaatac
     1081 cgcaccaata tccccgtcac gcacgctacg gataacctgc tcatcaacac aacgtactgc
     1141 ttcattcagc atcaacatca cacaccgttc agcaacctgc ggtgcggaga gtcgcccctg
     1201 cccttgtgcg ccaatgagcg ggtaaatggc aggatcgacc tgttttttgc ttttacgccc
     1261 tttctgacca taaagataga aaccccgacc attttttctg cctttgcgat cgtcgttcaa
     1321 aattgaagaa acaacatttg caggcgcgct aaaacgttct ccataagcgg cttccagtac
     1381 aggaataatt ttagtcccgg tgtcgattcc tacctcatcc aaaagttgga ttgggcctac
     1441 cggaaaacca aatttcacta gcgcggcatc aatgtgctct acccgttcac cttcggtcag
     1501 catgcggata gcttcattaa tgtaaggcgc taagatgcga ttgacgtaaa acccggcttt
     1561 gtcacgcacg acaatgggcg ttttgccctg ttttttcgcc agttttaccg tagtggcgat
     1621 ggtttgcgcc gatgtacccg catgaggaat aatctctacc agcggcattt tttccaccgg
     1681 actgaagaaa tgcaggccaa taacttgctc aggtcgcgcg gcgtgagcgg cgatatcacc
     1741 aatcggtaaa gatgacgtgt tcgaagcaaa aatggtatga gcggcgcaat tttgctccac
     1801 ttccgccacc atctgttgtt ttaattcgag attttcaaac accgcttcaa taatcagatc
     1861 gcgatgggca aagccgcgat agtccgtcgt tccggagatt aatgccagct gtttgtcacg
     1921 ttcgctggct ttgagatgac gacggcgaac tttgccctcc agctgatccc aactgtactt
     1981 cagcgcatga tttatgccct gcgggttgat atctttaatt ctgaccggaa gccccgcttt
     2041 acaagcagtg acataagcaa taccgccgcc catcaagcca ccacctaaaa ttcccacgct
     2101 gtttaatggc gcaggcggtg catcactacc gggatctttc ttcacgtccg tactggcaaa
     2161 aaagatacta cgcaaagctt gcgattgcgg cgtcatcgcc agttcgccaa aggcgcgagc
     2221 ttcagcgtca tagccgctgc tggtgccctg cgctaatccc gtttcaacaa cctccaggat
     2281 gcgttctgtc gccggataat tgccttgagt tttgtgttct gttttcttgc cgaccatttt
     2341 gaacagcagc gcacgaccta acggccccgc cagaatacgc tcgcgtacag gtagagggcg
     2401 ggaagatggg cgatccttct tcgccagctc aacagcggct tccagcaaaa tggagtgcgg
     2461 aacaacgtca tccaccagcc ccagctttaa tgcctgtttc gcccgaagtt gttttccggt
     2521 gagaatcatc tctaatgctg tactgacgcc tatcagacgc ggtaaacgct gggtgccgcc
     2581 tgaaccgggt aacaatccaa gttgtacttc aggcaaaccg agcaccgttt taggatcgtc
     2641 agtacaaacg cgaccgtggc acgccagcgc cagctccagc ccaccgccca ggcaagcgcc
     2701 atgaatagcc gcgataaccg gaatgggcaa agcatgaatc tccgccatca actgttggcc
     2761 ctgccgcgcc agtgcttccg cttcttgcgc cgttttgcag ttgccaatca tgttgatgtc
     2821 tgctccggcg ataaaattgt ccggtttcgc ggagataaac accacgccgc gcaactcttt
     2881 gttttcacgg agttgcttaa taatggcgcg cacctgcgag gcaaattccg ccttcagggt
     2941 attcattttc tcatccggta cgtcgatggt gatgatagca atgttgtcca gacgaacatt
     3001 aagagtaaac gctgatgcca tttccattat tccgcctcca gaaccattgc cgcgccaagc
     3061 ccaccggcag cacaggcggt aactaaacca aatccaccgc cgcgacggcg aagttcatgc
     3121 aatgtctggg taatcatccg cgcgccggtc gccgcgaagg gatgtccgta agcaatcgaa
     3181 ccgccaagca cgttaaattt gctatcgtcc acttcgccag tggcatgtgc acgccccagt
     3241 acttcacgag caaaacgttc actacccagc aactgaatat tcgccagtgt ctgagctgca
     3301 aaggcttcgt gcatatcgat caatgtcagt tcgctcatcg tcaaaccggc acgttccagc
     3361 gccagcggtg ttgaccaggc tggaccgagc aacatgtcct gccagacatc aatcgcagta
     3421 aatgcgtagc tgcgcagata ccccagcggc accagcccta attctttcgc ccgggattca
     3481 gtcatcagga tcaccgctgc cgcgccatcg gtcagcggcg tactgtttgc cgccgttacc
     3541 gttccgtgtt tgcgatcaaa cgccgggcgc agctttgcgt aatcggcaag cgaggaatta
     3601 ccgcgaatat tgttgtcttc gacaagcggt tgtttataag gagggataaa ggcagtcatc
     3661 acctcttctt tgagttttcc gtctgaccat gcctgagcgg cacgctgatg cgaacggtgc
     3721 gctaatgcat cttgctgttc tcgggtgatg ccgtaggttt tcgccatttg ttcggcggtg
     3781 tcgcccatcc gcaagccagt agaatattct gctaccgcag gcggtacggg cattaagtcg
     3841 cgcaaacgca ggcgagagaa gagtttcagg cgctggctca tggttcgagc tttgttgaca
     3901 tcaaccagca cgtacgccag ttttttactg acgccaattg gcaatacgga agaggaatct
     3961 gccccaccgg caatccccgc tcggatagtt cccgccatca ggctttctgc gacgtttgca
     4021 accgcttgga aactggtagc gcaagcgcgg ctgacgctgt aagcatcggt atgcacattc
     4081 attccggtac cgagaacaat ttcacgcgca atgttggggg cttcaggcat ttgtacgacc
     4141 tgaccaaaga ccagctgttc aatcacttca gcggggatct cgctgcgtgc cagcagttcg
     4201 cctaccacca tcttccctaa atcaaccgtg ggaatgccat gaaaagccgt cgcctggcgg
     4261 gcaaaaggcg tacgtaaacc gctaacaatg gcgatacgat cgccctggcg ggtaaccagt
     4321 ggtaaaacct gacccataac actcccctgt aaaaaaataa ataaagtggt ctgacctgat
     4381 catagtctta accatttttt tacatttagc caagcggaga aaaaggaaag tgggagctat
     4441 gacacagtga agacagaaga aagaaaagaa aacgcccctg ccagcaactg acaggggcgt
     4501 acgtacccga gaggaattaa cgcagaccca gctggaagat cagcatttca gcttcgcagg
     4561 caaaagtgaa atcgatatcc aggcgcacac cgtcagactc ttcagtgaaa gtcggggaga
     4621 ttttgcacgg ttcggatttc acgctacggg atttttcagt cagcgccgcc agcgtttgtt
     4681 ctgcttctgc gcggtttgca aacacgcggc tgtaagacgc ggtgcagtcg gagttgtcca
     4741 taatggtgcc aacatccata cagcagcaaa ccggggtttc atcagcacta catttactca
     4801 tcgttgattt cctctgtatg tgcacccaag gtgccagata aacgttgtgg atattttacg
     4861 cttccggaaa gtgctgctcc agttgttaat tctgcaaaat cggataagtg accgaaatca
     4921 cacttaaaaa tgatctaaaa caaaattcac ccgaatccgc gagtgcgcaa cctccaaatt
     4981 ttgccagctg gatcgcgttt cttagatcat atttgaaaaa agatggaaac atacttgcaa
     5041 cattctagct ggtccgacct atactctcgc cactggtctg atttctaaga tgtacctcag
     5101 accctacact tcgcgctcct gttacagcac gtaacatagt ttgtataaaa ataaatcatt
     5161 gaggttatgg tcatgagcca gaaaaccctg tttacaaagt ctgctctcgc agtcgcagtg
     5221 gcacttatct ccacccaggc ctggtcggca ggctttcagt taaacgaatt ttcttcctct
     5281 ggcctgggcc gggcttattc aggggaaggc gcaattgccg atgatgcagg taacgttagc
     5341 cgtaaccccg cattgattac catgtttgac cgcccgacat tttctgcggg tgcggtttat
     5401 attgacccgg atgtgaatat cagcggaacg tctccgtctg gtcgtagcct gaaagccgat
     5461 aacatcgcgc ctacggcatg ggttccgaac atgcactttg ttgcaccgat taacgaccaa
     5521 tttggttggg gcgcttctat tacctctaac tatggtctgg caacagagtt taatgatact
     5581 tatgcaggcg gctctgtcgg gggtacaact gaccttgaaa ccatgaacct gaacttaagc
     5641 ggtgcgtatc gcttaaataa tgcatggagc tttggtcttg gtttcaacgc catctacgct
     5701 cgcgcgaaaa ttgaacgttt cgcaggcgat ctggggcagt tggttgctgg tcagattatg
     5761 caatctcctg ccgggcaaac tcctcaaggg caagcattgg cagctaccgc caacggtatc
     5821 gacagtaata ccaaaatcgc tcatctgaac ggtaaccagt ggggctttgg ctggaacgcc
     5881 ggaatcctgt atgaactgga taaaaataac cgctatgcac tgacctaccg ttctgaagtg
     5941 aaaattgact tcaaaggtaa ctacagcagc gatcttaatc gtgcgtttaa taactacggt
     6001 ttgccaattc ctaccgcgac aggtggcgca acgcaatcgg gttatctgac gctgaacctg
     6061 cctgaaatgt gggaagtgtc aggttataac cgtgttgatc cacagtgggc gattcactat
     6121 agcctggctt acaccagctg gagtcagttc cagcagctga aagcgacctc aaccagtggc
     6181 gacacgctgt tccagaaaca tgaaggcttt aaagatgctt accgcatcgc gttgggtacc
     6241 acttattact acgatgataa ctggaccttc cgtaccggta tcgcctttga tgacagccca
     6301 gttccggctc agaatcgttc tatctccatt ccggaccagg accgtttctg gctgagtgca
     6361 ggtacgactt acgcatttaa taaagatgct tcagtcgacg ttggtgtttc ttatatgcac
     6421 ggtcagagcg tgaaaattaa cgaaggccca taccagttcg agtctgaagg taaagcctgg
     6481 ctgttcggta ctaactttaa ctacgcgttc tgataacgcg ttcgcctgga taaagtcacc
     6541 tgctatgcag gtgactttaa ctcccccact tcaccgaaag tagtttcctc attgcttgcc
     6601 tcctgagttt tgcaaacagc ctgatggcag cttgcccttt caaatcagta agtggtatcc
     6661 acagacaaac ctgaagggaa atgcatattt tcaagcgttc tgctcgtcct tctcaaagag
     6721 tttacttttc tgcatttcca ggatactcaa cccccctggc tattgtgcgc ttatacactc
     6781 aaattaaaga t
//
LOCUS       DAZPAB010000192         6784 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZPAB010000192 DAZPAB010000000
VERSION     DAZPAB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.192"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            188..442
                     /gene="bssS"
                     /locus_tag="ACHUPG_003432"
     CDS             188..442
                     /gene="bssS"
                     /locus_tag="ACHUPG_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR0862908.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            557..1675
                     /gene="solA"
                     /locus_tag="ACHUPG_003433"
     CDS             557..1675
                     /gene="solA"
                     /locus_tag="ACHUPG_003433"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR0862909.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            1723..1836
                     /locus_tag="ACHUPG_003434"
     CDS             1723..1836
                     /locus_tag="ACHUPG_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFR0862910.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2097..2663
                     /locus_tag="ACHUPG_003435"
     CDS             2097..2663
                     /locus_tag="ACHUPG_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR0862911.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2667..3242
                     /locus_tag="ACHUPG_003436"
     CDS             2667..3242
                     /locus_tag="ACHUPG_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR0862912.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3284..4336)
                     /locus_tag="ACHUPG_003437"
     CDS             complement(3284..4336)
                     /locus_tag="ACHUPG_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR0862913.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4561..5481
                     /gene="lpxL"
                     /locus_tag="ACHUPG_003438"
     CDS             4561..5481
                     /gene="lpxL"
                     /locus_tag="ACHUPG_003438"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR0862914.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            5653..5838
                     /locus_tag="ACHUPG_003439"
                     /pseudo
     CDS             5653..5838
                     /locus_tag="ACHUPG_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5874..>6784
                     /locus_tag="ACHUPG_003440"
     CDS             5874..>6784
                     /locus_tag="ACHUPG_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0862915.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGII"
BASE COUNT         1755 a         1619 c         1613 g         1797 t
ORIGIN      
        1 atgctaaaaa aatatgaatg acatgctgtt ttattgttca tataaatctt aaaagcagaa
       61 atttatctgt tccccccttt aaaaattcgg tgaataccct tacttattgg tatactgaag
      121 ttgcttcaga taaaaacgca ttgaacctcg aataacgttg tctagtaaca cgaattaggg
      181 ggccatgatg gaaaaaaata atgaagtcat tcagactcat ccgctcgtag ggtgggacat
      241 cagcaccgtt gatagctatg atgcgctgat gttgcgtttg cactaccaga ccccaaataa
      301 gtccgagcag gaagggactg aagttggtca gacgctctgg ttaaccactg atgttgccag
      361 acaatttatt tcgatattag aagcaggaat cgccaaaatt gaatccggtg atttcccggt
      421 aaacgagtat cggcgtcatt aacatgacat gaccatccac ccaaaaggca cctcaccggt
      481 gcctttacca tttctgggtt gtatatctgc attcagttaa ttactctgct taaaccatga
      541 gagcagagag aaaaaaatga aatacgatct catcattatt ggcagcggtt ccgtaggcgc
      601 tgccgccggg tattatgcaa cccgcgccgg tttaaacgtg ctaatgaccg acgcccatat
      661 gccaccgcat caacacggca gccaccacgg cgatacgcga ttaattcgcc atgcttatgg
      721 tgaaggcgaa aagtatgtcc cgttggtcct ccgcgcgcaa acgctgtggg atgaactttc
      781 ccgccacaac gaagatgatc ccatttttgt acgctctggt gtcattaacc tcggcccggc
      841 tgactccgca tttctcgcca acgtcgccca cagcgccgaa cagtggcaac tcaacgttga
      901 aaagctcgat gcgcaaggga ttatggcccg ctggccagaa atacgcgtcc cggacaacta
      961 catcggctta tttgagactg attccggttt tttgcgcagc gaactggcga ttaaaacctg
     1021 gatccaactg gcgaaggaag cgggctgtgc gcaactgttc aactgcccgg tcaccgcaat
     1081 tcgtcatgac gatgatggcg taactattga aacggttgac ggtgagtatc aggcgaaaaa
     1141 agcgattgtc tgcgcgggaa catgggtaaa agacctgctc ccggagctgc ctgtccagcc
     1201 cgtacgtaaa gtatttgcct ggtatcaggc cgatggccgc tatagcgtga agaataaatt
     1261 cccggcgttt accggtgaac tgcccaatgg cgatcaatat tatggttttc cggcagaaaa
     1321 cgacgcgttg aagattggca aacataacgg aggccaggtt atccattcag cggatgaacg
     1381 tgttccgttt gcggaagtgg ccagtgatgg gtcagaagcc ttcccgttct tgcgcaatgt
     1441 attgccgggt atcggttgct gcctgtacgg cgctgcctgc acctatgata attcgcctga
     1501 cgaagatttt attatcgata ccctacccgg ccacgataat acactgctca ttaccggcct
     1561 gagtgggcac ggttttaaat ttgcgtcagt tttaggggaa atagctgccg attttgcgca
     1621 agaccaaaaa agcgattttg atttgacgcc attcaggctt tcccgcttcc aataatcatc
     1681 attacggcct ccagaatgag gccgttttta caggagtatt taatgcgtcg gctgttgcac
     1741 tatctcatca ataatattcg cgagcatctg atgctttatc tttttctctg gggattgttg
     1801 gcaattatgg atctgatcta tgtgttttat ttttagtttt ccgcactgac ataaactgac
     1861 aaatatttaa tctccctttc atttagcgtg aaaaattgaa tgagattatt cctgcttcta
     1921 cagcactcac cagacatctc tgatgctgaa cccactaaac ttatgaagat tctttcatta
     1981 ttaattcaaa attattgaaa taaaaataaa tatcattcaa tttgttattt ttgtgttgca
     2041 attcattctc cgacacgcca ttttctttct ctcgcaaatt cgtattggat gcacttatgt
     2101 cattcacaaa tacccctgaa cgctatggtg ttatatctgc ggccttccat tggctaagcg
     2161 caattattgt ctacggcatg tttgctttag gtctgtggat ggtcacgctc agttattacg
     2221 atggctggta tcacaaagca cccgaactgc ataaaagcat cggtattttg ttaatgatgg
     2281 ggctggttat tcgcgttctg tggcgtgtca tatctccacc gccgggtccg ctgccgagtt
     2341 attcgcccat gactcgtctt gccgcaaaag ctggacatct cgcattgtgc ctattgctgt
     2401 tcgccatcgg catcagcggc tatctgatct caactgccga tggtaaaccg atcagcgttt
     2461 ttggctggtt tgacgtcccc gcgacccttg ccgacgctgg cgcacaggca gactttgccg
     2521 gtgccctgca tttttggctc gcgtggagcg tcgtagtact gtctgttatg cacggattta
     2581 tggccctgaa gcatcatttc atcgataaag acgacactct gaagcgcatg ctgggaaagt
     2641 cgtcatctga ctatggagta taaaaaatga aaaaaagcct gcttggttta accttcgcgt
     2701 ccctgatgtt ctctgccggt tcagcggttg ccgccgatta caaaattgac aaagaaggtc
     2761 agcacgcctt tgttaacttc cgcatacagc atcttggcta tagctggtta tatggcacct
     2821 ttaaagattt cgacggtact tttacctttg atgaaaaaaa tccggctgcc gataaagtga
     2881 atgtgacaat taacaccacc agcgtcgata ctaaccacgc cgaacgcgat aaacatcttc
     2941 gcagtgcgga ttttctcaat accacaaaat atccacaggc aacattcacc tccaccagcg
     3001 tgaagaaaga cggtgacgaa ctggatatta ccggcgatct gacgctgaat ggcgtaacca
     3061 aacccgtcac gctggaagcg aaattaattg gtcagggcga cgacccatgg ggcggtaaac
     3121 gtgcaggctt tgaggccgaa ggcaaaatta agctcaaaga cttcaatatc aagacagatt
     3181 taggtccagc ttctcaggaa gtggatctga ttatttcagt ggaaggcgta cagcagaagt
     3241 aagagtgaaa tgttgccgga tggcacgcat ccggcaatga tatttattct gttggatcag
     3301 gaatacccag tgttgtattc agacgtccac gagacttatt aaagatctta ttgccatttt
     3361 cacgtcctgc ccgacggcgt cgctgttctt ctggcggtaa cgcgctttct tcgcagcaaa
     3421 tctcactaca acaacctttg tatttttccg cgcatactgg acactgaata aacagcaggt
     3481 ggcagccatc atttttacag ttggtatggc tgtcgcacgg cgcgccgcac tggtggcaat
     3541 gcgcgataat ctcatccgat atacgttcgc ccatccgctc gtcaaaaaca aaatttttgc
     3601 caataaaacg caccggcaag ccctgctcgc gcgccttacg ggcgtattca ataattccgc
     3661 cctcgatatg ccagactttg ttgaatccgt tatgtttcat ccaggcactg gccttttcac
     3721 aacgaatgcc gccggtgcag tacatgacga tttttttatc tttatgtgcc tgcatcatct
     3781 cgactgcttt tggcagctgc tcacggaagg tatctgccgg aatttcgagc gcgttttcaa
     3841 agtgccccac ttcatactca tagtggttac gcatgtcgat aaatagtgca tcgggatcgt
     3901 caagcatggc gttcacttcc gccgcttgca gatactcacc aacattgctg gcatcaaagt
     3961 gaggatcgtc aataccgtcg gcaacaatgc gatcgcgtac cttcatgcgc agtacccaga
     4021 aggatttccc gtcatcatcc aacgcgatat tcaggcgtaa accctctaaa gccgggtcga
     4081 aggcatagag ctgcgcgcga aatgtttcaa cattgctcgc aggtacgctg atttgcgcgt
     4141 taatgccctc atgcgccaga tacactcgcc caaaaacatt cagcgcggta aacagctgat
     4201 ataaagcgtc acgggtcgcc ttaggatcgg cgatgtggaa atacttgtaa aacgaaatgg
     4261 tggttcgcgg ttcgctctca gccaacattt tggcttttaa cgcgtcgttg gaaatgcggt
     4321 tgtgtaacac tggcatggtg tacggttcct gcgagatggg aaagtaaaaa tccgcggcat
     4381 gatatagcaa ttatcgataa ttaacatcca cacattttac gctacatttg cgcattaaaa
     4441 attatttgtt atttacaagc gcggcaattt cgcccagtct tcagccacaa attttggttg
     4501 cgggcgaaaa aatgcgacaa tacatacaat tgcccgtata ggttgaaaaa caggattgat
     4561 atgacgaatc tacccaagtt ctccaccgca ctgcttcatc cgcgttattg gttaacctgg
     4621 ttgggtattg gcgtactttg gttggtcgtg caattgccct acccgattat ctaccgcctc
     4681 ggttgtggat taggaaaact ggcgttacgt tttatgaaac gacgcgcaaa aattgtgcat
     4741 cgcaacctgg aactgtgctt cccggaaatg agcgaacaag aacgccgtaa aatggtggtg
     4801 aagaatttcg aatccgttgg catgggcctg atggaaaccg gtatggcgtg gttctggccg
     4861 gaccgccgaa tcgcccgctg gacggaagtg atcggcatgg aacacattcg tgacgtgcag
     4921 gcgcaaaaac gcggcatcct gttagttggc atccattttc tgacactgga gctgggcgcg
     4981 cggcagtttg gtatgcagga accggggatt ggcgtttacc gcccgaacga taatccactg
     5041 attgactggc tacaaacctg gggccgtttg cgctcaaata aatcgatgct cgaccgcaaa
     5101 gatttaaaag gcatgattaa agccctgaaa aaaggcgaag tggtctggta cgcaccagat
     5161 catgattacg gcccgcgctc aagcgttttc gtcccgttat ttgccgttga gcaggctgcg
     5221 accacgaccg gaacctggat gctggcacgg atgtccggcg catgtctggt gcccttcgtt
     5281 ccacgccgta agccagatgg caaagggtat caattgatta tgctgccacc agagtgttct
     5341 ccgccactgg atgatgccga aactaccgcc gcgtggatga acaaagtggt cgaaaaatgc
     5401 atcatgatgg caccagagca gtatatgtgg ttacaccgtc gctttaaaac acgcccggaa
     5461 ggcgttcctt cacgctatta aatctcccat gccggatgct tcagaatggc atccggcatt
     5521 accacagcaa atccccctga tttagcgata aaagctctct ggattgcgcc ccctggaagt
     5581 cgggcgcata attagtgtgt ttatcttttc ttcttatgtt caccgcgcct ggcgcaccaa
     5641 cagcggattg ctatgtcacc ctgtgaaaat gacaccccta taaactggaa acgaaacctg
     5701 atcgtcgcct ggctaggctg ttttcttacc ggggccgcct tcagtctggt aatgcccttc
     5761 ttacccctct acgttgagca gcttggtgtt accggccact ccgccctgaa tatgtggtcc
     5821 ggtattgtct tcagtataat gggttcctaa acacatcatt ttaggtattg aatatgagac
     5881 gggcaatcag ctacatcaga ttttcaagtg aacgacaact gaaaggggac tctgtacgcc
     5941 gacaatctaa gctggttact gactggcttg ataagaaccc tgagttttac ttggattcat
     6001 ccttgagctt taaagatttg gggaaaagtg cttttagtgg taagcacctg aaaggtggtc
     6061 taggtgactt tttaaccgct atcgagaaag ggcttgttaa agctggtgat acgctactaa
     6121 tcgagagttt agaccgtctg tcacgtcagg atattgatat tgcttctgag ctattacgcc
     6181 gtatcttaag ggctggtgta gatgtagtca cactgagcga tggagaacat tacacacgag
     6241 aaagtttaaa agacccgtta gccctgatta aaagcattct gataatgcag cgggcgcatg
     6301 aggaaagttt gagaaagtct gagcgtgtcc aagctgcatg gaaccgtaaa aaagaactca
     6361 tctccgaagg tatcaaggta tctaggcgtt gtcctgcatg gttaaggctg aatgatgacc
     6421 gtcgaacatt cacaatcatt cctgataaag tggaagttgt taaacgagca tttgacctga
     6481 gattacaggg tttaagcttt tgggctatca ccagaacttt aaacgatgaa ggtcacttgt
     6541 cattgaatca atacacaccc aaacaaaagg ggtggtctga tactgctgta aagaaactgc
     6601 tcagaaatcg tgctgtgatt ggatgtttca cacctgctgg acgtgaagaa gttcagggtt
     6661 actaccccgc aataatcagt gagtcactat tctatcgagt acagcaactg aacacagggc
     6721 aatatggtag ggcttctgta tcaagtaatc cgctatcagt caacttgttc aggggtatca
     6781 tcaa
//
LOCUS       DAZPAB010000193         6756 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZPAB010000193 DAZPAB010000000
VERSION     DAZPAB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.193"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..1107
                     /gene="pdeF"
                     /locus_tag="ACHUPG_003441"
     CDS             <1..1107
                     /gene="pdeF"
                     /locus_tag="ACHUPG_003441"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR0862916.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
     gene            complement(1146..2687)
                     /gene="ppx"
                     /locus_tag="ACHUPG_003442"
     CDS             complement(1146..2687)
                     /gene="ppx"
                     /locus_tag="ACHUPG_003442"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR0862917.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(2692..4758)
                     /gene="ppk1"
                     /locus_tag="ACHUPG_003443"
     CDS             complement(2692..4758)
                     /gene="ppk1"
                     /locus_tag="ACHUPG_003443"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR0862918.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(4930..5568)
                     /gene="purN"
                     /locus_tag="ACHUPG_003444"
     CDS             complement(4930..5568)
                     /gene="purN"
                     /locus_tag="ACHUPG_003444"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR0862919.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(5568..6605)
                     /gene="purM"
                     /locus_tag="ACHUPG_003445"
     CDS             complement(5568..6605)
                     /gene="purM"
                     /locus_tag="ACHUPG_003445"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR0862920.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
BASE COUNT         1663 a         1723 c         1682 g         1688 t
ORIGIN      
        1 aagcaaaaac tttctcactg gctgtcaccc ttgctggaac cgggtgaaga tgtttatcag
       61 ctttcgggta acgatctcgc gctgcgactg aatacagaat cgcaccagga gcgcattacc
      121 gcactggata gtcatctcaa gcaatttcgt ttcttttggg atgggatgcc gatgcaaccg
      181 cagattggcg tcagttactg ctatgtgcgc tcgccagtga atcatatcta cctgctgctg
      241 ggagagctaa atacggtcgc cgaactttcc atcgtgacca acgccccgga aaatatgcag
      301 cgttgcgggg caatgtattt gcaacgcgaa ttgaaagata aagtcgcgat gatgaatcga
      361 ctacagcagg cgctggaaca caaccatttt ttcctgatgg cccagccgat taccggtatg
      421 cgtggtgatg tttaccatga aattcttctg cgcatgaaag gtgagaatga tgaactgatc
      481 ggccccggta gcttcttgcc ggtcgcgcac gaatttggtt tatcgtcgag tatcgacatg
      541 tgggtcattg agcatacgct gcaatttatg gctgaaaaca gagcgaagat gcccgctcac
      601 cgttttgcta ttaatctgtc tccaacctcg gtatgtcagg ctcgttttcc tgttgaagtc
      661 agtcagttgc tggctaaata tcagattgaa gcgtggcaac ttatttttga agtcaccgaa
      721 agtaatgctc tgaccaatgt taagcaggcg caaatcacct tgcagcatct tcaggaatta
      781 ggctgccaga ttgcgattga tgatttcggc accggctacg ccagctatgc acggcttaaa
      841 aatgtgaatg ccgatctgct taaaattgac ggcagcttta tccgcaatat tgtgtcaaat
      901 agtctggatt atcagattgt ggcgtcgatt tgccatctgg cgcgaatgaa gaaaatgcgg
      961 gtagtggcag agtacgttga aaacgaagag atccgcgagg cggtgctctc tttggggatc
     1021 gattatatgc agggttatct tattggtaag ccgcaaccgt taattgatac gctgaatgaa
     1081 atcgaaccca ttcgcgaaag tgcctgaata atgcgggccg acatttctcg tcggcccgca
     1141 aagcattaag cggcgatttc tggggtactt tcttcttcaa ttttcaaccg ccagccagcc
     1201 acgccttccc agtattcttg ctccttttcc agatcaagca gtaccagcgc attctgacta
     1261 aaccagtcat gcgggaaacg cagtgtccag tgactgtcat cagtaatcag tgtcaatgtt
     1321 ggcggtgtgg tggttgcctg acgttgattg tttagtaata cgccaaggcg caatagctgt
     1381 atcagtggca ggaactgttt cttcttaaac aaggtaaagc gcggcagatc gtcgagctta
     1441 atcgctttac ggtgatagcg caccagtgtc gccatcatca gctgctgttc ctgattaaaa
     1501 cccggcaagt cactgttttg cagaatataa gcggagtggc gatgcaaacc gctgtggttg
     1561 atattcaacc cgacctcatg cagcatggcg gcccatcgca gtagcgcctc cagttgcgga
     1621 tgcgccagct tcggttgctg ttcccgccac tgttcgtaca tttgcatagt ggtatccagc
     1681 actcgtcggg cctgttcgct gtctatgtga tactggttgg cgaggctgct ggcggtgcga
     1741 ctacgcacat cctgatgacg gaaacgtcct tccatttcat acagtacgcc ttcgcgtaac
     1801 gccccgtcag aaaggcgcag ttcacggatg gctaaagcat caaacacacc acataaaatc
     1861 gccagtccag gaacgaagac tgttttccgc tcttcggaaa gacccggtaa actcagcgat
     1921 gcgaaattac ggtgacgtaa aatttctttt accagttttt ccagacgttc cggggtaatt
     1981 atcccgtctt tctcgcccat ttccattaac acttcatggg cggcttttat ggtaccggaa
     2041 gcgcccattg caacgttcca gccctgaata cggaattgcc aggttaaagt ttccagtttt
     2101 tgtgctgccg ccatgcgagc gcgctgaaaa ttctctttat tgatgacccc gccaggaaaa
     2161 taaagctggg caaagctgac acaacccatc cggcggcttt caacgagaat aggttcgaaa
     2221 ttttcaccaa tcaccagttc cgtagatccg ccgccaatat caataaccag tttgcgacct
     2281 ttttccggtt gggtatgttc cacgcccata aaaatcagac gggcttcttc attaccggaa
     2341 ataatttcaa tcgggtaggg aatgaccttt tccgcgcgtt tcagaaagtc agtggcgttc
     2401 agcgcctgac gcagcgtatg ggtgccgact atacagacgc tggcaggaga aaacccttgt
     2461 agccgttcgg caaacagcga cagacagttc aaaccgcgcg tcattgcctc ttcactcaac
     2521 atattatctg gccccaggcc gtccgccaga tgcacccgct gtttcaggcg gccaataatc
     2581 tgcatggcac catctaccac acgggctatg accatgtgaa aactgtttga accaagatcg
     2641 accgcagcaa actcctgcgg acgaggggat ttatcgtgta ttggcatagg gttattcagg
     2701 ttgttcgagt gatttgatgt agtcgtaaat cgccaactgc gcccgtactt tgcggcgatt
     2761 gccgcgggga acgtagcgat tactgagttc tttatcgata taacgtgctt tgaccgtatc
     2821 gctgaacaat atgtcgatga tgtccagcac ccgctgcttc aggcgcggat cgagcagcgg
     2881 tgtcgccact tcaatacgat aatcaatatt gcgcgtcatc cagtcggcgg aagaaaggta
     2941 gaccttttta tcgccgccat tttcaaaaat ataaacccgg tcatgttcaa ggtaacggtc
     3001 aacaatactg atggcacgaa tgttgtcgct aatgccttcc agattgggga tcagcgaaca
     3061 cattccgcga accagcagat taaccggtac gccggagctg gaggccgcat acagacgatc
     3121 aaccaggcct ttatcgacaa ggttatttag cttcagggtg ataccactgg gcagcccttg
     3181 ctgcgcgttg gcgatttcgc ggtctaccat ttcatataac agacggcgag agttttgcgg
     3241 tgacaccatt aaataatcaa atgtcaccgg gcggtatggg ttttcaataa agttaaatac
     3301 ccgccgtact tcgttggtga tacgcgcatc tgcggtcagc aacgaatagt cagtataaag
     3361 acgcgcggtt ttttcgttaa agttcccggt cccgatgtgt gcgtaacgga ccacttcacc
     3421 gttttcttta cgtgaaatca ggaacagttt ggcgtgaatt ttcagccccg gcgcagagaa
     3481 gataacgtgc acgcctgctt cggtcaggcg cttcgcccag tgaatgttgg cttcttcgtc
     3541 gaaacgcgcc tgtaactcaa ccaccacagt gactttctta ccgttatgtg cggcgtggat
     3601 cattgagtcg atgatgcgtg aatctttcgc cacgcggtaa atgttaattt taatcgccag
     3661 tacgctcggg tcgaacgaag cctgacgcag cagttccagc acatgctcaa aggtgtgata
     3721 aggataatag agcaacacat cgcgttcgcg aatggcatca aaaccattgc ggaactgggc
     3781 tttatcaaac caaatatggc gtaaacgcgg cagtggtttg ttcaccagat tggctttgcc
     3841 gacattgggg aaattaataa agtctttaaa attatgataa cgaccgccgg ggacgatgga
     3901 gtcgtagcgg gaaatagtca gtttttcgcg taacacttca accagcgcat tgggcatatc
     3961 gcgctgataa acaaaacgca ccggctcagc agttaaacgc tgcttgagac tggaagacat
     4021 caactccatc aggctggctt ccatctcatg cactaaatcg tattcggcat cgcgggtcat
     4081 cttcattgaa taggcattca gcgcgtcata atcaaagaag cctttgaaaa tatcatcaag
     4141 gcagtaacgc agaatgttat ccagaagaat catcggcttg cgtcgacgcg gcgcttctgg
     4201 cggtaaattc acaaagcgcg gcactttatc tgatgggatc tccagcagcg cgtaacggat
     4261 gctatcgcta cggataattt ccaccgccag ataggtgtaa tcatctttca ggaactgcac
     4321 taagtcagtg tcaggattga ttaaaatcgg cgtaatgtgc tgacgcagat actgcttaaa
     4381 ataatgacgc agccagtttt gttgattgac ggagagctgg cgttcattaa tcaggaagat
     4441 ctggttgcgc gccatctcca gcaatagctc gttgtagagg ccgtcgaatt cctgatcggc
     4501 tttcagcacc cgggactgaa ttttgcccag taaatggcgg gaatgagcgt tggagccttg
     4561 ttcttcgcta ataatgatgc gtcgcttcag ttcagcgaag cggactttat agaactcatc
     4621 aaggttattg gaatagatcc ccaggaaacg catcctttca atcagcgggt tagatttgtc
     4681 cgccgcttcc tgaagcacgc gttcattgaa cgataaccaa ctgagctctt tttcgatgta
     4741 tagcttttcc tgacccatta ccacttttac tccgttttat tcacgggaca ctgcctggat
     4801 attatggcga gcaatattgg cagatgtcca actatgccag aaaaggggga aaaaaactgc
     4861 tggagcttgc gatttacagg tgaattgaaa aacgccagcg gcagagctgg cgctttaact
     4921 acgggggcat tactcgtcgg cagcgtagcc ctgcggcggc agacgttgac catccagcca
     4981 cgcggcgttt tcgtgcattt tcagacgacc atcggcaaac cagctaatca ccagtggata
     5041 aatagcgtgt tcctgggttt gcacgcgggc ggtgatgtca tcttccgtat caccagcaaa
     5101 taccgggact ttcgcctgta aaataaccgg gccaccgtcc agttcatcgg tgacgaaatg
     5161 caccgatgta ccgtgctctt catcgccatt ttccagcgcc tgacgatggg tgtgtaatcc
     5221 gggatatttc ggcagcagag aagggtgaat gttcagcaaa cgcccggcat agtgggagac
     5281 aaacgccggg ctgagaatgc gcataaaacc agccagcacg accacatcgg gtgcgtacat
     5341 gtcgatttca tgaatcaact cccggtcata ggcttcacga ctgtcaaacg cgctggcgat
     5401 gagcgtatgc gttgcaatac ccgcctggcg ggcgcgttca aggccgaacg cgtcggcctt
     5461 attgctgaaa actgcccgta cggtgccttt aattttgttg gttttacagg cgtcaataat
     5521 tgcctgtaaa ttacttccgt tgccggaaat aagcaccaca atattcatta ttcgataacc
     5581 acgcgttgtt cggaatcaga ggctttgatg ataccgattt tccacgcgtt ttcaccgttg
     5641 gcatttagca gggcgagggc tttgtccact tccggagcag gcagggcgat aatcatcccg
     5701 acgccgcagt tgaaggtgcg atacatttca tggcgctcaa cgttacctgc cgtttgcagc
     5761 cagttgaaca cttccggcca ctgccaggaa gattcatcaa tcactgcctg ggtattatct
     5821 ggcaatacgc gcggaatgtt ttcccagaag ccgccgccgg tcaggtgcgc aatggcatgc
     5881 acatcgacct tttcaatcaa ctccagcact gacttcacgt aaatgcgggt cggtgccagc
     5941 agatgatcgg ctaatggctt accatcaagt tcggtggttt gcggatcaca accgctgact
     6001 tcaagaattt tgcgcaccag cgaatagccg ttcgagtgcg gaccgctgga accgagtgca
     6061 atcagcacat cgccgtcgct gactttagag ccgtcgatga tttctgattt ttctaccacg
     6121 cccacgcaga aacccgcgac atcgtaatct tcaccgtgat acatccccgg catttctgcc
     6181 gtttcgccac ccaccagaga acagcccgat tgcagacaac cttccgcgat gccgctgatc
     6241 accgctgaag cggtatcaac atccagtttt ccggttgcgt aatagtcgag gaaaaacagc
     6301 ggttccgcac cttgcaccac caggtcatta acgcacatgg cgaccagatc aataccaatg
     6361 gtgtcgtgac gttttaagtc cattgccaga cgcagcttgg tacctacgcc gtcagtgccg
     6421 gaaaccagca cgggttcacg atatttttgc ggcaatgcac acagcgcacc gaagccgccc
     6481 agaccgccca tcacttccgg acgacgcgtt ttcttcacta cgcctttgat tcttccaacc
     6541 agagcattac ccgcgtcaat atcaacaccg gcatctttgt agctaagaga ggttttatcg
     6601 gtcactgctt gggtccccac gcgttacttg cggtagaaaa ataaaattcg gcgcaattct
     6661 aacagggaaa gcaaacgttt gcgagactgt tttacacaac ctttttgcac gtcttttccc
     6721 cgggcgcgcg gcgaaagaag acttgtgcca gggcaa
//
LOCUS       DAZPAB010000194         6598 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZPAB010000194 DAZPAB010000000
VERSION     DAZPAB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.194"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..1416
                     /locus_tag="ACHUPG_003446"
     CDS             <1..1416
                     /locus_tag="ACHUPG_003446"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR0862921.1"
                     /translation="DRNNLAPRIVHLGFGAFHRAHQGVYADILATEHFSDWGYYEVNL
                     IGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQMDGLETVLAAMCEPQI
                     AIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATGVIVEALARRKAAGLP
                     AFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFPSTMVDRIVPAVTEDT
                     LAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPYEEMKLRM
                     LNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAPTLKVQGVDLQDYANR
                     LIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLALGVAGWMRYVG
                     GVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLPDNSLFTAKVT
                     EAYLSLLAHGAKATVAKYSVK"
     gene            complement(1452..1655)
                     /gene="ydfZ"
                     /locus_tag="ACHUPG_003447"
     CDS             complement(1452..1655)
                     /gene="ydfZ"
                     /locus_tag="ACHUPG_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR0862922.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1833..2519)
                     /gene="rspR"
                     /locus_tag="ACHUPG_003448"
                     /gene_synonym="ydfH"
     CDS             complement(1833..2519)
                     /gene="rspR"
                     /locus_tag="ACHUPG_003448"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR0862923.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2608..3354)
                     /gene="ydfG"
                     /locus_tag="ACHUPG_003449"
     CDS             complement(2608..3354)
                     /gene="ydfG"
                     /locus_tag="ACHUPG_003449"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR0862924.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3491..5536
                     /gene="dcp"
                     /locus_tag="ACHUPG_003450"
     CDS             3491..5536
                     /gene="dcp"
                     /locus_tag="ACHUPG_003450"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR0862925.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5581..6099)
                     /locus_tag="ACHUPG_003451"
     CDS             complement(5581..6099)
                     /locus_tag="ACHUPG_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR0862926.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            6375..>6598
                     /gene="ydeI"
                     /locus_tag="ACHUPG_003452"
     CDS             6375..>6598
                     /gene="ydeI"
                     /locus_tag="ACHUPG_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFR0862927.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKG"
BASE COUNT         1661 a         1567 c         1672 g         1698 t
ORIGIN      
        1 gatcgtaata acctggcccc aagaattgtt catttaggct ttggtgcatt tcaccgtgcg
       61 catcagggcg tgtatgccga tattcttgct acagaacatt tcagtgactg gggatattat
      121 gaggtcaact taatcggcgg cgaacagcaa attgccgatt tacaacagca agataatctt
      181 tataccgttg cggaaatgtc ggccgatgcg tggacggctc gcgtcgttgg cgtcgttaaa
      241 aaagccttgc acgtacagat ggatggctta gaaaccgtgt tggctgcgat gtgtgaaccg
      301 caaatcgcga ttgtctctct gacaatcacc gaaaaagggt atttccactc tccggcgacc
      361 ggacagttaa tgctcgagca cccgatggtc gctgccgacg tgcaaaatcc ccaccagccg
      421 aaaacagcaa caggggtgat tgttgaggcg ctggctcgcc gtaaagcggc aggacttccc
      481 gcatttaccg tcatgtcatg tgacaacatg ccagaaaacg gtcatgttat gcgtgacgtt
      541 gtcacttcct acgcgcaagc cgttgatgta aaactggcac aatggatcga agataacgtg
      601 actttcccat caacaatggt ggaccgtatt gtgcccgcag tgacagagga tacgctggcg
      661 aaaatcgaac aacttaccgg tgtgcgcgat cctgctggcg ttgcctgtga acctttccgc
      721 cagtgggtta tcgaagataa ctttgtagcc ggacgtccgg aatgggaaaa agcgggagcc
      781 gaactggtta gtgatgtgct gccttatgaa gagatgaagt tgcgcatgct caacggcagt
      841 cattcattcc tggcatatct gggttatctt gctggatatc agcacattaa tgactgtatg
      901 gaagatgaac attatcgtca tgcggcgtat ggcatgatgt tgcaggaaca agcgccgacg
      961 ctgaaagtgc agggcgttga tttgcaagat tacgctaacc gattaattgc acgctatagc
     1021 aacccggcgt tacgtcatcg aacctggcag attgcgatgg atggcagcca gaaattgcca
     1081 cagcggatgt tggattctgt tcgctggcat ctggcgcatg acagcaagtt cgatctgctg
     1141 gcgctgggcg tcgcgggttg gatgcgttat gtcggtggtg ttgatgaaca gggaaatccg
     1201 atagaaatca gtgacccact gttacctgtt attcagaagg ctgtacaaag tagtgccgaa
     1261 gggaaagcgc gcgtccagtc attgctggcg attaaggcaa tctttggtga tgatttgcca
     1321 gacaatagct tgtttactgc aaaagtgacg gaagcgtact tgtctttatt agcgcatggc
     1381 gcgaaagcga ccgtggcgaa atattccgtg aagtaaaaaa agcaatgccg ccatcggcgg
     1441 cattcacaca cttagttcat gccgagacga atcaggtcca gaggtgccag tttctcttca
     1501 caaccttcaa caactacggt ttttccgcgg cggatttgct cagcggtcag accttcagta
     1561 tcaatcgaca ggatcttgcc agtgtgaccg gtgccgctaa ccataacacg gctgccagtg
     1621 gtgattgcgt tacggttacg atcgtaggtc gtcataggtg ttttctcctt tctgatttac
     1681 agtcgaagcc cacgtaacgg gcgcaatata aatactcctg tcgagtgaaa tattttttgt
     1741 ttttgatcaa gctcacatct tttttgatgc atgcatgagc cgtaaggcgg ggggaaaagc
     1801 ctctcatccc ggatgggatg agagtaaatg aattactctt cgctaaacca gtcgctgttt
     1861 tcctggcgga tctggcgcac ggattcgctg atttcctgca aatgttgcgt cattgcacgt
     1921 tctaccgcat cgccatcacg tttttgcagg gcagagaaaa tatcaagatg ctggcgtaac
     1981 agcatttctg gtggagaaac atggtcgaaa ctcatatagc gcacgcgatc aacggtcgct
     2041 ttcaggttct caatggtatc ccacgccagt tgacagtcgg caatctgcgt caggagttga
     2101 tggaagttgt catcaagttc aaaaaaatca tccagttgct tgcgctcaat ggcaatgcgt
     2161 tgctggtgaa gattttgttc cagttggtag cactggcttt cggtaatcat gctcgccgcc
     2221 cgacgcgcca ccgcgcactc aatggcctga cggataaaac tgccgttgcg cacctgggcc
     2281 atggaaattt tgttgacgta gctgccacgc tgcggacgaa tttgaatcag gccgttttcc
     2341 gccagtttaa taaaggcttc acgaaccggc tggcgtgaca cattgaaacg aacagaaact
     2401 tctttttccg acaacggtgt gcctggtgca atcaggcaat ggacaatgtc gcgacgaaga
     2461 atacgataaa tctgctgatt gacaggctgt gtgggattaa gttgcgtttc gacggtcatt
     2521 cgttcttgct tgtgagtgag ttaactgcat gagtctacca cttttttgtg acaagcaata
     2581 acccggcagt tacgccgggt ataaaaatta ctgacggtgg acattcagtc cggcatagct
     2641 ttgggtaacc ggcatcattt ccagggtatt gatattgacg tgagcaggca gcgttgacac
     2701 ccaccagacg gcttcgctga catcttctgg cgtcaatgca acggtatttt gataggtttt
     2761 ttccgcttta ccgtcatcgc ctttaaagcg gacattggaa aactcggtgc cacccaccag
     2821 acccggttcg atgtcggtga cgcgcaccgc cgtaccatgc agatccgtac gcagattcag
     2881 gctaaactga cgaacaaacg ctttcgtcgc accgtaaacg ttaccaccgg catacggcca
     2941 gctgcctgcc gttgagccaa tgttaataat atgaccatga ttacgttcaa ccatacccgg
     3001 taagacggcg cgcgtcatat atactaggcc tttgttgttg gtatcaatca tcgtttccca
     3061 gtcttcaacg ctggctttat gcgcaggctc catgcccaac gccaggccgg cattatttac
     3121 caggatatca atattgctcc actcggcagg gagcgatgcc agcatctctt caatagcggc
     3181 gcggttgcga acgtccagtt gggcgatata cagattatct cccagttcgt ctgttaactc
     3241 ctgcaaccgc tcctggcgac ggccagtggc gataacttta tgcccttgtt gaataaaacg
     3301 acgagtaatg cattcaccaa aacctgccgt tgctccagtt actaaaacga tcatctcact
     3361 gttcctcaac gcttttgtgt gtcaataaca tagcacgtga tgcttaagag cgggtaatac
     3421 tggtttgcaa gattgcactc cgttatcagt aagcctacgc tatggaacac ctcgcgttca
     3481 ggagaagaaa atgacaacaa tgaatccttt ccttgtgcaa agcacactgc cgtatctggc
     3541 tcctcatttt gatcaaattg ccaatcatca ctatcgcccg gcattcgatg agggaatgca
     3601 acaaaagcgg gcagaaattg ctgccatcgc gcttaacccg caaacgcctg atttcaacaa
     3661 tactattctg gcactggaac aaagcggaga attaattacc cgcgttacca gcgtcttttt
     3721 tgcgatgact gcggcgcata ccaatgatga attacagcgt cttgatgaac agttttccgc
     3781 tgaactggcg gaactggcta atgatatcta tctgaacggt gaattattcg cgcgggtaga
     3841 tgctgtctgg cagcgccgtg aatccctggg gcttgatagt gaatccatcc gcctggtgga
     3901 ggttattcat caacgttttg tccttgccgg agccaaactt gcacaagctg ataaagcaaa
     3961 attaaaagta ctgaatacag aagctgcgac cctgaccagc cagtttaacc agcggttact
     4021 tgcagcaaat aaatccggcg gtctggttgt gaacgatatc gcgcagctgg caggaatgag
     4081 tgagcaagag attgcgctgg cggcagaggc ggctcgcgag aaaggtctgg ataacaaatg
     4141 gctgattccg ctgctgaata ccacccaaca accggcgctt gccgaaatgc gcgatcgtgc
     4201 gacgcgtgaa aaactgttta ttgcgggctg gacgcgagcg gaaaaaaatg atgccaatga
     4261 tacccgcgct atcattcaac gtctggtaga gattcgcgca cagcaggcga aactgcttgg
     4321 ttttcctcat tatgccgcat ggaaaatcgc cgatcagatg gcaaaaacgc cagaagcagc
     4381 actcaacttt atgcgggaaa ttgttccagc ggcgcgtcaa cgtgcgagcg atgaattagc
     4441 ctccatacag gcggttatcg ataagcagca gggcgggttt agcgcgcagc cgtgggactg
     4501 ggcattttat gcggaacagg tacggcggga gaaatttgat cttgatgagg cgcagctcaa
     4561 gccatatttt gaattaaaca cggtgttaaa tgaaggtgta ttctggaccg cgaatcagct
     4621 cttcggtatt aagtttgtcg aacgttttga tattccggtc taccatcctg acgttcgtgt
     4681 gtgggaaatt tttgatcata atggcgtggg actggcgtta ttttacggtg atttcttcgc
     4741 ccgtgattca aaaagcggcg gtgcatggat gggcaatttt gttgagcaat caacgcttaa
     4801 tgaaacgcat ccggtaattt ataacgtctg caattatcag aaacccgctg ccggtgagcc
     4861 tgcgttgtta ctctgggatg atgtaataac cttattccat gaatttggtc atacgctgca
     4921 cggccttttt gcccgccagc gttatgccac gctttccggc accaacacgc cgcgtgattt
     4981 tgtcgaattt ccgtcgcaaa tcaacgaaca ctgggcaacg catccgcagg tattcgctcg
     5041 ctacgcccgg cattatcaga gcggggcagc aatgcctgac gaactgcaac agaaaatgcg
     5101 taatgccagc ctgttcaaca aagggtatga gatgagcgaa ctgcttagcg ccgcacttct
     5161 cgatatgcgc tggcattgcc tggaagaaaa cgaagcaatg caggatgtcg atgattttga
     5221 attgcgggcg ctggtggcgg aaaatatgga tcttcctgct ataccgccac gctatcgcag
     5281 cagttatttc gcccatattt ttggtggcgg atatgctgca ggttattacg cttatctgtg
     5341 gacgcaaatg ttggccgatg atggttacca gtggtttgtt gagcagggcg gattaacgcg
     5401 tgaaaatggg cagcgttttc gcgaggcgat cctttccaga ggtaacagcg aagatctgga
     5461 acgcctgcat cgacaatggc gtggtaaggc tcctcagatt atgccgatgc tgcaacatcg
     5521 tggcttgaac atataaacac caccaatggc tggcctggag atatcgccag gccagcctgg
     5581 ttatagcagt aattgcagca gctgggcgag ggcaaacctc accgctaaag ctaataccgt
     5641 ttcgcagtcg ccagcaaaat gcataaccgc agtgtagttc tggcctttaa tatgccacgc
     5701 gaaccagacg gtacccgctg gcgtaccatc ttcaccgccc tccggtccgc cgtagccggt
     5761 aatggcaata ctgacatcag catccgcacg ctctatggca cctgttgcca tttctgctgc
     5821 cactttctca ctcaccgcag aatatcgttc aagagactgc tggcttacgc tgaggatttt
     5881 catctttgcc tgatcggtga aagtaacaaa gcctgcaccg taaaatttgg gtgtatcttc
     5941 agctgcacac agggcgctag ccaacttacc gccggtgcat gattcggcgg tagttaagcg
     6001 taattgatcc gcgataagtc tttgactcaa cgcggatgtc aggttttcaa tagtgtcggt
     6061 atctgcgagt tgtacaattt tatctttatt aatgttcact gttttttgtc cacgctatta
     6121 tatgtaaagg tgatttgctt tttctgtatg ctcgtccaat ataataattt tcaaacatat
     6181 aaatccgatt attctgaaaa catgtttaag actcttcaat cttcttaact ttagtgagtc
     6241 aaattctctg aatatttgac agctagtggg ctgattttaa atgccttata acaattggca
     6301 gtattcttat ttggcaatta taaaaatagc aaccagactt aataagtcaa accaaacgca
     6361 attaaggagt tagtatgaaa tttcagtcca ttgtattagc aagttttctt gtcatgcctt
     6421 atgcgttggc agatgaccag ggcggtttaa aacaggatgc agcgccaccg ccgcctcatg
     6481 caatagaaga tggctatcgc ggaaccgatg atgcaaaaaa aatgaccgtt gatttcgcaa
     6541 aaaacatgca cgatggcgct tcggtttcac tacgcggtaa tttgatttct cacaaagg
//
LOCUS       DAZPAB010000195         6597 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZPAB010000195 DAZPAB010000000
VERSION     DAZPAB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.195"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..1076
                     /locus_tag="ACHUPG_003453"
     CDS             <1..1076
                     /locus_tag="ACHUPG_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAPES1 domain-containing protein"
                     /protein_id="HFR0862928.1"
                     /translation="AEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTA
                     STDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPV
                     NVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVA
                     GQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYV
                     SYVHDLPGGIRVSLSIDILYFITSSRKSVLFWILTALILLNMVRMHFRLYQNVSRENI
                     SDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAIT
                     LHDAYKASDERLYVNKQNKNSRS"
     gene            1119..1622
                     /gene="yeaK"
                     /locus_tag="ACHUPG_003454"
     CDS             1119..1622
                     /gene="yeaK"
                     /locus_tag="ACHUPG_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFR0862929.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1623..1727)
                     /gene="yoaI"
                     /locus_tag="ACHUPG_003455"
     CDS             complement(1623..1727)
                     /gene="yoaI"
                     /locus_tag="ACHUPG_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFR0862930.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1897..2343
                     /locus_tag="ACHUPG_003456"
     CDS             1897..2343
                     /locus_tag="ACHUPG_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFR0862931.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2300..3118)
                     /gene="nimR"
                     /locus_tag="ACHUPG_003457"
                     /pseudo
     CDS             complement(2300..3118)
                     /gene="nimR"
                     /locus_tag="ACHUPG_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            3215..4395
                     /gene="nimT"
                     /locus_tag="ACHUPG_003458"
                     /pseudo
     CDS             3215..4395
                     /gene="nimT"
                     /locus_tag="ACHUPG_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4450..4797
                     /locus_tag="ACHUPG_003459"
     CDS             4450..4797
                     /locus_tag="ACHUPG_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFR0862932.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4819..5073)
                     /gene="yoaF"
                     /locus_tag="ACHUPG_003460"
     CDS             complement(4819..5073)
                     /gene="yoaF"
                     /locus_tag="ACHUPG_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFR0862933.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5256..6281
                     /gene="dgcP"
                     /locus_tag="ACHUPG_003461"
     CDS             5256..6281
                     /gene="dgcP"
                     /locus_tag="ACHUPG_003461"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFR0862934.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6314..6412
                     /locus_tag="ACHUPG_003462"
     CDS             6314..6412
                     /locus_tag="ACHUPG_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFR0862935.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6415..6489
                     /gene="yoaJ"
                     /locus_tag="ACHUPG_003463"
     CDS             6415..6489
                     /gene="yoaJ"
                     /locus_tag="ACHUPG_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFR0862936.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
BASE COUNT         1599 a         1599 c         1615 g         1784 t
ORIGIN      
        1 ctgctgagca gtcggaagtg tcgccagaac agcggcgcgc catctgcgag gcttttgagt
       61 ctgccaataa tacccatggc ttaaacctga ctgcccataa atacccgggc ttacgcggca
      121 cactacaaac cgcatccact gactgcgaca caattgtgga agctgcagca ctattacccg
      181 cttttgatca ggcagtggaa ggcaaccgcc accaggatga ttacggttca ggtcttggga
      241 tggccgaaga gaaatttcac tattatctcg atctcaatga ccgctatgtc tatttttatg
      301 agccggttaa tgttgaatac tttgcgatga ataactggtc cttcctgcag tcaggaagta
      361 ttggcatcga tcgcaaagat attgaaaagg tatttaccgg gcgtaccgta ttgtcgagca
      421 tttaccagga tcagcgtact aaacagaacg tgatgagttt gctgacgccg gtatatgtcg
      481 cagggcagct aaaagggatt gtgctgctgg atattaacaa aaacaatttg cggaatatct
      541 tttacactca tgaccgccct ctcctctggc gttttctcaa tgtcacgcta accgataccg
      601 attcggggcg cgacattatc atcaaccaga gcgaagataa tctgttccag tatgtcagtt
      661 acgtccatga cttacctggc ggcattcgtg tctcgttatc cattgatatt ctttacttta
      721 tcacgtcttc gaggaaaagc gttctgttct ggattttgac ggcgttaatt ttgctgaata
      781 tggtccggat gcacttccgt ttataccaaa atgtgtcgcg agaaaatatt agtgatgcga
      841 tgactggact gtataatcgc aaaattttaa cccctgaact ggagcagcgg ttgcagaaac
      901 tggtgcaatc cggttcttcg gtgatgttta ttgcaattga catggacaag ttaaagcaaa
      961 taaatgacac cctcggtcat caggaggggg atttagcgat tacgttacat gatgcgtata
     1021 aagcttccga tgagcgttta tatgtcaata agcagaacaa aaacagccgt tcatgataac
     1081 cttctgtggt tgtttgcttg taatctcagg agcgtgaaat gactgaaatg gctaaaggaa
     1141 gcgtgacgca tcagcagtta atcgctttat tatcacaaga aggtgctaac ttccgcgtcg
     1201 ttacccatga agcggtaggt aaatgtgaag cggtgtcaga aattcgtggt accgctctag
     1261 gccagggtgc taaggcactg gtatgtaaag tcaaaggcaa tggcgtaaat caacatgttc
     1321 tggcgatcct cgcagccgat caacaggccg atctgagcca actcgccagc catataggcg
     1381 gattacgcgc ttcgttggcc agcccggcgg aagttgatga actcacaggc tgtgtcttcg
     1441 gcgcgatccc ccctttcagc ttccatccaa aactcaaact ggttgccgac cctctactct
     1501 tcgaacgatt tgatgaaatc gccttcaatg cagggatgct ggataaatcc gttattttga
     1561 aaaccgcgga ttatttgcgc attgcgcaac cagaactggt caatttccgc cgcactgcgt
     1621 agctagccta tccgttcaat aagcagaacg gacaaaacca gcacaaccgc aatagcaaaa
     1681 aacgatgatg tgataatcag tgtctcgacg aacatttgat cgttcatagc atgcccccgg
     1741 gagatagctg ttttcacgtt attgttagcg tgcacaaatg gcagtttgat gacagttcgc
     1801 ctgatttttt atgcaaaaaa cgtaaagatt ttctacttcc ttcctgcagc aagcataaag
     1861 taagcaggct tattattttt tggcaaggaa accacgatgt ttgatgtcac tttgctgatc
     1921 ctgctcgggt tagctgcgct gggctttatc agtcataaca ccactgtcgc cgtttcaatt
     1981 ctggtgttga tcattgtccg cgtcacaccg ttaagcacct ttttcccgtg gattgaaaaa
     2041 caggggctaa gtatcgggat aatcattctg acgattggcg ttatggcccc catcgccagc
     2101 gggacgctac cgccctcaac cttgattcac tcgtttctga actggaaatc gctggtggca
     2161 attgcagtag gcgttattgt ctcctggctg ggtgggcgcg gcgtgacgtt aatgggcagc
     2221 cagccgcaac tggttgctgg gctgctggtg ggcactgttt taggcgtagc gctgtttcgc
     2281 ggcgtaccgg ttggcccgct tattgccgcg ggtctggttt cgctgattgt ggggaaacag
     2341 tagttaatcc cgcaatatat cggcctggcg tttgccccaa gcctttctta aacatagtaa
     2401 taaaggccgt tgttgaatca taccccagcg tatgagcaac tttctgtaca gtatcgcctt
     2461 tcactaatcc ctgaagcgcc ataatcagct gtaactgctg acgccactgg cgaaaactca
     2521 accccgtctc tttcactatc aggcgggcaa gattgcgttc actcatcgca aaaaagccag
     2581 cccattgccc caatgccccc cactcgacgg gccctttcgc catcatctcc accatcgtgc
     2641 ggattttagg atgagaagaa acgggcaggt gcagctgttg ttgtggctgc tgagggagtt
     2701 catcaaacag gacctgaatt aatctgcggg tcatcggctc agcacgctga gtcgtcgtcc
     2761 tgtttgccag ggttaaaatc aattcccggc ataagggcga tattttcaat gtgcaacatg
     2821 gtcggcattg tcactgcgct gggttcgata aacagaaaac agagttcagc gttagctgtc
     2881 acttgattgc tatgcttcac cccgcccggt atccaaaccg catattgtgg cggcaccatc
     2941 cacaaagcat tttccaccgt acaggtaatt gcaccatgta atgccagtat cagctgtcct
     3001 ttgcggtgct gatgtacagg actggttaac tcatccgttc cggcgtgaat acaaaatgcg
     3061 accgcggctt catggtgtcg gtgggttcat agccattgag attcaacctg tgcatcattt
     3121 tgtccgaact tagcgataat ttgtcatttt agcttgattc aacataacaa taaaaacggt
     3181 aaggtacagc ctcgtttgta acaatgagaa gcatatgacc tgttcaactt cattaagcgg
     3241 caaaaacagg attgtcctta tcgctggcat tctgatgatt gccacaacat tacgcgtcac
     3301 ctttaccggc gcagcgccgt tactggatac gattcgttcc gcttactcgc tgacgacagc
     3361 gcaaaccggc ttattaacca ccctgccatt attggccttt gcgctaatct cacctctggc
     3421 tgccccggtg gcgcgacgtt ttggtatgga acgtagcctg tttgccgcgt tacttttgat
     3481 ctgtgcaggt atcgcaattc gttctctccc ttcgccttac ttattatttg gcggtacagc
     3541 ggtcattggc ggtgggattg cattaggcaa tgtcttactg ccaggattaa ttaaacgcga
     3601 tttccctcat tccgtcgcca gacttaccgg cgcatattcc ctgacaatgg gagctgcagc
     3661 ggcactggga tcggctatgg tcgtgccgct ggctttgaac ggttttggct ggcaaggcgc
     3721 gttgttcatg ctgatgtgtt ttcctctgct ggctcttttt ttatggctgc cacagtggcg
     3781 aagtcagcaa catgcaaatt tgagtacctc acgcgcctta catactcggg gtatctggcg
     3841 ttcgccactt gcctggcagg taacattgtt tcttgggatc aactcactgg tctattacgt
     3901 gattattggc tggcttccgg cgatcctcat cagtcacggc tatagcgaag cacaggcggg
     3961 ttcactgcat ggtttgctgc aactagccac agcagctccc ggtttgctga tcgcactttt
     4021 cttacatcat gtgaaagacc agcgtggtat tgcagcgttc gttgccttga tgtgcgcagt
     4081 gggcgcggtt gggctctgct ttatgccagc gcacgcgatc acctggactc tgcttttcgg
     4141 ttttggttcc ggcgcaacaa tgatactggg gttgacgttc attggtcttc ggcaagttct
     4201 gcgcatcagg cggcggcact ctcggggatg gcacaatccg tcgggtattt gctggcagcc
     4261 tgtgggccgc cgctaatggg taaaatacac gatgctaacg gtaactggtc cgtaccactg
     4321 atgggtgttg caatactttc actactgatg gcgattttcg gactttgcgc cgggagagac
     4381 aaagaaattc gctaatatcc ggtgctatag tgacgtgaca aatcttgcgt gaaagggaga
     4441 acaaacacga tgaatattca gtgcaaacgc gtttatgatc cggctgaaca gagcgatggt
     4501 tatcgcgtac tggtcgaccg cctctggccg cgcggtatca aaaaaaccga tttagccctt
     4561 gatgagtggg ataaagaaat cacgccgtca acggaactgc gcaaagcctt tcacggcgaa
     4621 gtcgtcgatt ttgcaacctt tcgcgagcaa tatcgcgcag aactggcgca acacgagcaa
     4681 gaaggaaagc ggctggcgga catcgccaaa aaacagccgc tgaccctgct ctacgcagca
     4741 aaaaacacca cgcagaacca tgcgctggtg ctggccgact ggctacgtag cttgtgattt
     4801 tagtacggca tccggcggtt atttttcacc agccggatgg tcccgtcgcc acaatgccca
     4861 ttcatcaatc gtttcaccgc ccggtaattt gcaattgttg ctgacccctt gcgctgtctg
     4921 cactggaatg agcgtcccgc ccttctgctg gcaatagacc gacgccggat ttgccatacc
     4981 aatctgcggc ggtttaggtg cttctggctg agaaggggtt gaacaaccag ccaggaccag
     5041 caagcaaggc agaacaaaac tgataatttt catttattga tctcacatat ttatccaagc
     5101 ttagagtatc gcggtatcgt tttgttttgc agcactattt ttattacatt cactcaaaac
     5161 atattacgtc ttgtttcatc tttgttgatg atgttttatc atgcctgcaa agattaaata
     5221 atcagcactt acccgccgta tcctggagtt gttccgtgtc agatcagatt atcgcccgcg
     5281 tctcgcaatc ccttgccaaa gaacagtcac tggaaagtct ggtccgacag cttctggaga
     5341 tgctggaaat ggtcactgat atggaatcaa cctacctgac caaagtggat gtcgaagcgc
     5401 gcctgcagca tattatgttt gcccgtaaca gccagaaaat gcacatcccg gagaatttta
     5461 ccgtcttgtg ggattactcg ttatgcaaac gcgccattga tgaaaactgc tttttcagcg
     5521 atgaagtccc tgaccgttgg ggcgactgta ttgcggcacg caatcttggc atcaccacat
     5581 ttctgagcac gccaattcac ttaccggatg gatcattcta tggcacgctt tgcgccgcca
     5641 gcagtgagaa gcgccagtgg agtgaacgcg cggaacaggt tttacagtta ttcgccggac
     5701 tgattgcaca atatattcaa aaagaggcac tggttgaaca gctgcacgaa gccaatgctg
     5761 cgctgattgc gcaatcgtat accgactcgt taaccgggct accgaatcgg cgggcgattt
     5821 ttgaaaatct gacgacgctg ttttccctcg cccggcatct taaccataag ataatgatcg
     5881 cgtttatcga tctggataac ttcaaattaa tcaatgatcg ttttggtcat aatagtggcg
     5941 atctgtttct cattcaggtt ggcgagcgcc ttaatacgct ccagcaaaat ggcgaagtta
     6001 ttggtcgtct cggtggtgat gagtttttgg ttgtttcact aaacaacgag aatgcggata
     6061 tttcgtcgct gcgagaacgt attcaacagc aaatacgtgg agaatatcac ttaggtgatg
     6121 ttgatttgta ttatcccggt gccagtcttg gcatagtaga agtcgatcct gaaacaaccg
     6181 atgcagacag tgccctgcat gctgccgata ttgcgatgta tcaggagaaa aaacacaaac
     6241 agaaaacacc ttttgtcgcg catccagcgc tacattcctg aggcgtattc acatcctttt
     6301 gattggtgat aacatgcgaa tcggtattat ttttccggtt gtaatcttca ttacagcggt
     6361 cgtattttta gcatggtttt ttattggctg ctatgctgcc ccgggagcat aaagatgaaa
     6421 aaaacaacga ttattatgat gggtgtggcg attattgtcg tactcggcac tgagctggga
     6481 tggtggtaac gtcacctcta aaaaatagca aaggctgcct gtgtgcagcc tttgtgcaat
     6541 ttaagcgtta acttttaatc ttcctgtaga taaatagcac gacaatcgca ccaataa
//
LOCUS       DAZPAB010000196         6533 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZPAB010000196 DAZPAB010000000
VERSION     DAZPAB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.196"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..353)
                     /locus_tag="ACHUPG_003464"
     CDS             complement(<1..353)
                     /locus_tag="ACHUPG_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR0862937.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMA"
     gene            complement(350..934)
                     /locus_tag="ACHUPG_003465"
     CDS             complement(350..934)
                     /locus_tag="ACHUPG_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0862938.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            complement(921..1298)
                     /locus_tag="ACHUPG_003466"
     CDS             complement(921..1298)
                     /locus_tag="ACHUPG_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR0862939.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            complement(1310..1696)
                     /gene="gpFI"
                     /locus_tag="ACHUPG_003467"
     CDS             complement(1310..1696)
                     /gene="gpFI"
                     /locus_tag="ACHUPG_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR0862940.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            complement(1748..2776)
                     /locus_tag="ACHUPG_003468"
     CDS             complement(1748..2776)
                     /locus_tag="ACHUPG_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR0862941.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            complement(2834..3181)
                     /locus_tag="ACHUPG_003469"
     CDS             complement(2834..3181)
                     /locus_tag="ACHUPG_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR0862942.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            complement(3218..4723)
                     /locus_tag="ACHUPG_003470"
     CDS             complement(3218..4723)
                     /locus_tag="ACHUPG_003470"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR0862943.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATDAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            complement(4713..6306)
                     /locus_tag="ACHUPG_003471"
                     /pseudo
     CDS             complement(4713..6306)
                     /locus_tag="ACHUPG_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(6303..6506)
                     /locus_tag="ACHUPG_003472"
     CDS             complement(6303..6506)
                     /locus_tag="ACHUPG_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFR0862944.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
BASE COUNT         1421 a         2069 c         1574 g         1469 t
ORIGIN      
        1 gccatctcat tatcacgttg atagttaaaa ccgagtggga tcatggtgtc gatgagtccg
       61 gaaagcaccg gaatatcctt catggccgga aatatggtgt tctccatcca cgtatccagc
      121 tcagaatccg gcgcctgtgc acggacgaaa acggcaatat gcagaacagc ctgccagtcg
      181 tcctcatccg tcatttttcc ggtgtactgc gcatcactca gccacaccgc cacggcgggc
      241 agttcctgcg catcaataaa tgccggaagc ccgtcaaaaa acgtggcgct gtctccacac
      301 tgttcccgaa ggcgtgccag cacgctctga cggatttgtg tgtgtcggtt catttactca
      361 gatgtaacct cagttgttgt ttcagggcat accccagctg tttcggcatt tccgcagcaa
      421 tgatgcggtc gcgggcatct tcaaatgcct gtgtcagcgg tccggacatc gggattttca
      481 ccacatcaat ggggtaacga tttttgccgt caatacgccg catcacatgc cagcgaccat
      541 tcgccagttg ctgaataaac gcatcccgga aaaggtattt ccccactttc agtacgctgc
      601 cacgatgacg tgagttgctg ccatgccggg agcgggccag tctgacctgt gcggtaccca
      661 gcttaatggc gggcaggtta ccccggttaa cacgcatgcg ggcatacatt tttcctgagg
      721 ggctggcttt aagcagcctg acacgctccc tgaccgtttt caggggaata cctttcacct
      781 ggttatcccc ggcaacggta ttctgcgcaa cctgccgtgt ggcgacagaa acaatctttg
      841 ccgccacacg gtttaccgcc catgcgctgg tctgtggcac catgcgggta tcgaggctgt
      901 tcagattgcg gatggcattc tcaagtccct tcatcatatc gcctcattct ggcattattc
      961 tgtggggcag gactgccccg ccttaaccgg atataacagc atcccccgtc atccggagta
     1021 atacgatcca caaagaaaga atcatccccg atagttaacg tatcctgacg gcgtaactgc
     1081 gatatatctg ccgttttcac aaacaggcac ggtgaggaat cctcaagccg gatccccccg
     1141 gcaacaaaag aaatactttc gggatcatca aaaacccctg tcaggtgtgt cccctcaagt
     1201 tctccggacg taatgacggc gctgatcccc atatcacgga gaatgacttc attgacaccg
     1261 gcaagcgcag catcaaacag attatcgaaa ccgtccacga catctcctgt tactgccttt
     1321 ttgccagacc acctgccgcc atgctggcag ccacctcagc agaaacacga aacgacgttc
     1381 ccggtgaaac aaatatcact gaaccattac attttgtatg cagtgtcgcc agtgccacca
     1441 ccgtaacctg ccccgtttcg tctccggtaa caatgcctgg ctgttcagga actgactcgc
     1501 ctttatgcgc ggtcggatca tctttcgatg gtgagtacgt atccggctct gcgacctccc
     1561 ccgcatctcc aagctcttcc tccagttccg ccactcgctg cgcaatatct gccgcactac
     1621 cggagacatc cggttcacgc cccagcctct cagccagctc acaaagacgt ttcaggtttt
     1681 cttctttcgt agccataccc gctcctgatt gaatgaaaaa cgggagcatt tagctcccgc
     1741 tcacggatta tttcacctgc accaccacaa actcatccgg atccggcagc accatcagcg
     1801 gcgcggactg cgtcatggtg aattcacgcg ccggatcacc cacggtcagc cagtgtttcg
     1861 gataacggga agacgccacc acgccttcag acaatgcctg cgcatcctga atggcaccgt
     1921 agcaacgaat accctctgcg gccgtatttc ccagtaccag tgtgccgtcc ggcagataac
     1981 gtttttcggt accgtcctct gccacatagg acgtttttgc caccacgatt gccagatcac
     2041 cataataccc tttgaaggac accaccgccc ccaggtcttt cactgccgtt tccagctgtg
     2101 aatttgagcc acgacgggta tccagttttt cgcggaacag cttaaagcca ttcagcagac
     2161 gccagacctt accatccata atggcaatat tgatggtgcc ggaagcaaaa tcacagtacg
     2221 catccagatc atgcgtcgga tcaaaggtat cgcggtcctg agcgctccac tccgtccctc
     2281 ctgcctgcgt aatgttattt cccaccgacc ggccaaaatc gacctcaaca gtttcaaact
     2341 gctctccttc catggtgtat ttgccataca gcacggcatt caccgcctgc atctcttcca
     2401 cctggacaat ggcgtgctct tcctgtttga ggttatcggt gatgatacgc aggcgacggt
     2461 aagccgggcc gttcagctgg gccggatctt caccgggaag acgctccacc gcctgctggt
     2521 aattaaattc gtgtttcggc ttaacgtagc ccgggcgcag tacacgagtt tcaccgccac
     2581 ggtgacgcaa cacctttcct tcaacaaccg gggagacata ggccgccacc ggcgtttttc
     2641 cggtaatttt gtccagcatt acctcttcgg tatggaaatt caccgtacgg cggaaaaaca
     2701 gctccagaaa cagcgcacga aatttaactt tttgttcggt ataaccgagt aactggcggg
     2761 tcgtaaacaa tcccataaat cagttccttt cattcagaaa tcagtcaggc taccgcagtg
     2821 acctgataaa atgttacggc agcgctgcgt gactcagggc actgccggca aaggcattcg
     2881 cctttttgtg tgtatccact ctctcaggcc agtggatggc ctccgtcgca aaggtccccg
     2941 acttgtaata cgtcagcacc gtctctgtac cttcaagcgg cagtaccagt atgccaacca
     3001 catggcctgc cttctgtccg tcccagacca ccagtttccc ggtatcctca tccagcatca
     3061 ggggcgtcag agccggtgtt gccgaggaaa tcccgctgca gcctgtggcg gtatgggccg
     3121 gatcattacc ggcaaaaata cgtacttccg cacgctgttc agtgatggtt ttcgttacca
     3181 tattgtaaaa acctcatatt gatggtcagc actgacttca tggcatggcc atgagcattt
     3241 tcacgtccgc atcaccgtct gctgacgtct gtggcacgcc accctgtacc gctgccggtg
     3301 aatggttcgc catgaaatgt tcaaacatgg cggttgtgga tgcagaaacc ggttctgccc
     3361 tgcctgaggc tgccgccagt acagcccggg cattctccac ggtcatcccc ggacaggccg
     3421 ccagtttttc agcctgcgcc tctgcccctt ttgcctcatc cagggccata atctgatcac
     3481 gaagtgaggg tccggcattc gcctgcggtg aagcagccag aatcggacgg gctttttcca
     3541 ccgtcatctc cggcatcgcc gccagcgttg ccgccagttg ttcacgtcct ttcgcctctt
     3601 cacatgccat aatgcgatcg gcttcactct gcgcagatac accagattgt tgcggtgccg
     3661 ccacggccag aatcgcccgg gcctgctcaa cgctcatacc ctgttgccct gccagcatcg
     3721 tggccagttg ttcacgtcct ttcgcttcct gacacgtcag gatccccatc actcgctggt
     3781 tctcctgcgc ggcagcatcc gttgcagtta attgcggcat agtgcctcct gtatcgtgtg
     3841 tgttcagcgt cgcagccatc acgctgatgg cgtccgacgc attgattaat tcatccgcca
     3901 gttcggcctc aataccggac tgaccttcaa aaacggcggc ctctgttccc ataaccgcat
     3961 ccacagataa accggtatac atcgccactt tttcagcaaa catccggcgc gccgcatcaa
     4021 tgcgctgctg catgtcctgg cgaacctttg ctggcaggga ttcaaactga ttgccatcca
     4081 ccttgtgcga tccggcgtaa atcagcgtga tatccacacc ggcctgtgcc agatgaccgg
     4141 catagctgac atggcccatc atcaccccaa tggagccgat acgggatgtc tgggtaacca
     4201 gccgtcggga gcaggccgat gccagtaaca tggcggctga acaggccgtg tcattgcaca
     4261 gtgcccagac aggcttcagc tgccggagac ggtaaatcat gtcagcgcag tcaaacgcgc
     4321 cggtggcctg cccgccagaa ctgtcaatgt ccagcagtac gccccgtacc tgagtgtccg
     4381 ccattgcctg ctgaagacag gcgacaatgc cgtcatagcc agtcattccg gaaaatggcc
     4441 gcatgccacc cagccggtgt accagcgtgc cggtcaccgg cagtacagca ataccgttca
     4501 ccacccggta aacacgggca ggtcgtttgc ctccggccat gtactcgtcc gtttcagcca
     4561 gcatcccggg agcgtcaagc tgtacctgct gttgcggtac cgaaaggctt gctgcgccca
     4621 tctcacgccc gagcgcgcaa aagaaaaccc gcgcataggc gggctccaga agcagcggct
     4681 cattgaatgc cgcagcgata atatgtgaaa gattacgtct cacgtgatgt tgtctcctct
     4741 tccggcctgc gactctccgc tatctgctgc tgatacgcct gggctatcca caccggacgt
     4801 gagagtccgg ctttttcgcg ctctgcggat tccctgacct gctggcggaa aatgtcctga
     4861 taatcttcgc ccatcagtgc cagttctttc tcatacgtgc tcagtccggc ctcaatgcgc
     4921 atcaccgatt cctggacctc cttgagcccg tcaatggcca ttcttccgga gctaatccac
     4981 tctgcccgtg accaggctga acgcgcctgg taaaaatcaa aacgcgcccg tggcggacga
     5041 ataatccccc gaagaagcgc ctcttccagc cagcaggaaa acatctgcgt ggccagccgg
     5101 gccgcaataa attttcgccg ccccataaaa tagcgccacg actcattggc ggaggcgcgg
     5161 gcacttgagt aactgacctt cgagtaatca cgggacaact gttcgtagga aacgccaaga
     5221 ccggctgcaa tataccgcag cagcgcctgt tcaagcgccg aaaatccatt gtctgaatcc
     5281 tgcgctgtct gcaggttcag cgcatcgcca ggaaaaaagg tgcggaattt taacaccgcc
     5341 cagcgtcacg ttattcgtgt cataccagcg ggtgaacttc tccagaatat taataagcgg
     5401 attatcctgc tgaccttgcg gtgcccccgc gatatattca aatgcctttt cggtatcaag
     5461 ttcactttcg attgtggctg catacatcgc cttcacaatg gccgactgaa gctgcgtggc
     5521 ctgcagggag tccagcatct tcatccgctc cattacgctg taaaactgat tagccccacg
     5581 ggtctgcccg tcctccaccg gctcaaaaat atgcagcatg gccggacgcc cggtgggaag
     5641 ctcacgcggg atccgctccc agcgtccggc accagagaac ggaaaatcat cctcacagat
     5701 atggtacgca atcgcccgcc cataccgatc gacctcaacc ccggcccgca gaaagtggtt
     5761 ccccataccg tgtcctggcg tgtccacccg tttcggactc acggctttaa aacgcgtacg
     5821 gaataactgc gtggtttccg tatcccagac cggctgcaca aagatttcgc cgttaaaggc
     5881 atgcacgccc accccttcac ggataaattc cgtaaacgtg cgtttccctt ccacgtcgat
     5941 ctcgccaaac attccttctg cgtattccga ccaggctgct tccacctccc cgacaaaact
     6001 ttttgccgtg gtctcccgca tccccagcca acgccagttc agacggtagc tgataagaaa
     6061 catgtgcccg acgatatgat ccttatgcag ggccaccgca ttagccgcga taccgttatt
     6121 gcgcaccaga tcatctgccc gggcattccc cagacgcaat gcaggcaaca gggccgcatc
     6181 ggcactctgc gccggtggta accactctgc catttgcccg ccaaatcctg cgccgcctcc
     6241 gttgtagctg agactctcac gaagcggaac gccgttcaca tcaatcagga caggcgttcg
     6301 tttcataacc tcactcccag cggtcggcgg cgtcgggtcg tacccagaac cgactccgca
     6361 tcgttgatcg cacggttaag ctcattcagg gacgctgccg tatattcgat cctgcgaccg
     6421 tctttctgta ccgacacaac ccgtttaccg gttaataaat caaggcgcgc ctgacgcagc
     6481 gcctgcagtt cagcgactgt aaccattcac tcctccggac agcttcgctg cca
//
LOCUS       DAZPAB010000197         6520 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZPAB010000197 DAZPAB010000000
VERSION     DAZPAB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.197"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..115
                     /locus_tag="ACHUPG_003473"
     CDS             <1..115
                     /locus_tag="ACHUPG_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862945.1"
                     /translation="LDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            439..1827
                     /gene="narU"
                     /locus_tag="ACHUPG_003474"
     CDS             439..1827
                     /gene="narU"
                     /locus_tag="ACHUPG_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR0862946.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1909..5649
                     /gene="narZ"
                     /locus_tag="ACHUPG_003475"
                     /pseudo
     CDS             1909..5649
                     /gene="narZ"
                     /locus_tag="ACHUPG_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5646..>6520
                     /gene="narH"
                     /locus_tag="ACHUPG_003476"
     CDS             5646..>6520
                     /gene="narH"
                     /locus_tag="ACHUPG_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR0862947.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1558 a         1713 c         1690 g         1559 t
ORIGIN      
        1 cttagatgaa ataaacaata cttactataa taacgattat tacaatcagg caataagaga
       61 ggaaccgata gactttctgg acagatcgtt ttcctccaat tcatggcctt tttagtgaat
      121 aaatcatcac taattctata agtgtctggt cttgttgaga atatagttaa tatgtaaccc
      181 tttagattca cgatctgaag ggttgaaaca tgacatacgt tcaaagatta acccccgttt
      241 gaaggttagc ttgttagtta catttagtaa cacatatctg gctacttaat ccccattctg
      301 cccccgcggt tacgctttct tatctattct ttcaatcagc acttcaccca attcatcctt
      361 aattctaata actggctatt gcgccttacc tcctgttttg tcttttttca ttaccaatgt
      421 gtgcatatga ggaacaatat ggcactgcaa aatgagaaaa atagtcgtta tcttttacgc
      481 gactggaaac cagaaaatcc ggccttctgg gaaaataaag gaaaacatat tgctcgaaga
      541 aacctctgga tatcagtcag ttgtctactt cttgccttct gtgtctggat gctatttagc
      601 gcagttaccg ttaatctcaa taaaatcggt tttaatttta ctaccgatca actcttttta
      661 ttaaccgcat taccctccgt ttctggcgca ttattgcgtg ttccctactc ctttatggtg
      721 cctatattcg gtggacgccg atggccggtt tttagtactg caatcctgat tattccttgc
      781 gtctggctcg gaattgccgt gcaaaatccg aatactcctt ttgggatatt tatcgttatc
      841 gctttgctat gcggttttgc aggtgcaaac tttgcttcga gcatgggcaa tatcagtttc
      901 ttctttccaa aagccaaaca agggagcgct cttgggatta atggcggatt aggaaactta
      961 ggtgtaagtg taatgcagct ggttgcaccg ctggtcattt ttgtacctgt atttgccttt
     1021 ctcggcgtca atggcgtacc gcaggccgac ggttcggtga tgtcgctggc gaatgccgca
     1081 tggatttggg taccgctact ggcgattgcc acgatcgccg cctggtcagg gatgaatgat
     1141 atcgccagtt cacgcgcctc aattgccgac cagctccctg tcttacaacg cctgcatctc
     1201 tggctgctga gcctgcttta ccttgccacc ttcggttcgt ttatcggttt ttctgcgggt
     1261 tttgccatgc tggcaaaaac ccagttcccg gatgtgaata ttctgcgcct ggcgttcttt
     1321 ggcccattta ttggtgccat cgcgcggtcg gttggtggtg ctatttccga taaattcggc
     1381 ggcgtgcggg tgacgttgat caactttatt tttatggcga ttttcagtgc cctgctgttc
     1441 cttaccttac cgggcacagg ctccggtaat ttcatcgcct tttacgccgt atttatgggg
     1501 ctgtttctga ccgcgggtct gggaagtggt tctactttcc agatgatcgc cgtcatcttt
     1561 cgccagataa ccatttatcg ggtgaagatg aaaggcggta gtgatgagca agctcagaga
     1621 gaagccgtca ccgaaacggc ggcaacgctg ggctttatct cagccattgg cgcagtgggc
     1681 ggctttttta ttccgcaggc atttggcatg tcgctcaata tgaccggctc tccggtcggc
     1741 gcgatgaaag tgtttttaat cttctacatc gtttgtgtgc tgctgacctg gctggtttat
     1801 ggtcggcgga agttcagcca aaaataagag atcgatgcct gatgtacaac catgtcgcat
     1861 ccgacaaggc atcagatccc aattcattcc tggagcagga gtcatgtcat gagtaaactt
     1921 ttggatcgct ttcgctactt caaacaaaag ggcgaaacct ttgccgatgg tcacggacag
     1981 gtgatgcata gcaaccgcga ctgggaggac agctatcgcc agcgttggca gttcgacaaa
     2041 atcgtgcgtt ccacccacgg cgttaactgt acaggctcct gtagctggaa aatctacgtt
     2101 aaaaatggtc tggtgacctg ggaaatccaa cagaccgact acccgcgcac tcgccctgac
     2161 ctgcccaatc atgaacctcg cggctgcccg cgtggcgcaa gttactcctg gtatctttac
     2221 agcgctaacc gcctgaaata cccgctcatt cgtaaacgac tgattgaact gtggcgcgaa
     2281 gccctcaaac aacacagcga tccggtactg gcgtgggcat cgattatgaa tgatccgcaa
     2341 aagtgcctga gctacaaaca agtgcgtggg cgcggcgggt ttatccgctc caactggcag
     2401 gaactaaacc agctgattgc cgccgctaac gtctggacca tcaaaaccta cggcccggat
     2461 cgcgttgccg gtttctcgcc gatcccggcg atgtcgatgg tttcttacgc cgccggaacg
     2521 cgttatctgt cgctgcttgg cggcacctgt taaagtttct acgactggta ttgcgacctg
     2581 ccgcccgcct cgccgatgac ctggggcgag caaaccgacg taccggaatc tgccgactgg
     2641 tataactcca gctacatcat cgcttggggg tctaacgtac cgcagacacg tacgccggac
     2701 gcccacttct ttaccgaagt acgctacaaa ggcactaaaa ccatcgccat tacccctgac
     2761 tactctgaag tggccaaatt gtgcgaccag tggctggcac cgaaacaagg cactgatagc
     2821 gccctggcga tggcaatggg gcatgtgatt ttaaaagagt ttcatctcga taatcccagc
     2881 gactacttta tcaactactg ccgccgctac agcgacatgc cgatgctggt aatgctggag
     2941 cctcgcgacg atggtagcta cgttcccggg cgcatgatcc gcgcatctga cctggtggat
     3001 ggactgggcg aaagcaacaa tccgcagtgg aaaaccgtag cagttaatac cgcaggtgaa
     3061 ttggtagtgc cgaacggttc gattggtttc cgctggggag aaaaaggcaa atggaatctg
     3121 gaatccattg ccgccggtac ggaaaccgaa ttgtcgttaa ccctgctcgg tcaacatgac
     3181 gctgttgcag gcgtggcctt cccctacttt ggcggcattg aaaatccgca ttttcgcagc
     3241 gtaaaacaca atccggtgct ggtgcgccaa ttgcccgtta aaaacctgac gttagtcgat
     3301 ggcaacacct gtccggtggt cagcgtttat gatttggtac tggcgaatta cggcctcgat
     3361 cgcgggctgg aagatgaaaa cagcgcgaaa gattacgctg aaatcaaacc gtacacccca
     3421 gcctggggtg agcaaattac cggcgtaccg cgccagtata ttgaaaccat cgcccgcgaa
     3481 tttgccgata ccgcccataa aacgcatggg cgctcaatga ttatcctcgg cgcaggtgtt
     3541 aaccactggt atcacatgga catgaactac cgcgggatga tcaatatgct gatcttctgc
     3601 ggttgtgtcg ggcaaagcgg tggcggctgg gcacactatg tcggccagga aaaactgcgc
     3661 ccacaaaccg gctggttgcc actggccttt gcgctcgact ggaaccgacc accgcgccag
     3721 atgaacagca cctcgttttt ctacaatcat tccagccaat ggcgctatga aaaagtctct
     3781 gctcaggagt tactttcacc gctcgccgat gccagtaagt acagcggtca tctgattgat
     3841 ttcaacgttc gcgccgaacg tatgggctgg ctaccttctg cgccgcagct ggggcgtaac
     3901 ccgctcggga ttaaagctga agccgacaag gccggattat cccccacaga atttaccgcc
     3961 caggcgctga aatcgggcga tttacgtatg gcctgcgaac aaccagatag cggcagcaat
     4021 catccgcgta atttgtttgt ctggcgctct aacctgcttg actcctccgg caaaggccac
     4081 gagtatatac agaagtatct gctggggacc gaaagcggga ttcagggcga ggaactcggt
     4141 gccagcgacg ggatcaaacc ggaagaagtc gagtggcaaa ctgcggcgat tgaaggcaag
     4201 ctcgacctgc tggtgacgct cgacttccgc atgtccagta cctgcctgtt ctccgatatc
     4261 gttctgccca ccgccacctg gtatgaaaaa gacgatatga acacctcgga tatgcatcca
     4321 tttattcatc cgctttctgc ggcggtcgat ccggcctggg aatcacgcag cgactgggaa
     4381 atctacaaag gtattgccaa agcattttcg caagtgtgcg tgggccatct tggcaaagaa
     4441 accgacgtgg tattacaacc actgctgcat gactctccgg cagagctctc acagccgtgt
     4501 gaagtgctcg actggcgcaa aggcgaatgc gatctgatcc cgggtaaaac cgcgccgaat
     4561 attgtggcgg tggagcgcga ctaccctgct acgtatgaac gctttacctc gctcgggcca
     4621 ttgatggaca aacttggcaa tggcggtaaa gggatttcgt ggaataccca ggatgaaatc
     4681 gatttcctcg gcaaactcaa ttacaccaag cgtgatggcc cggcacaggg gcgtccgctg
     4741 attgacaccg ccattgacgc ttcagaagtg attctggcac tggcaccgga aaccaacggt
     4801 catgttgcag tcaaagcgtg gcaggcgctg ggcgagatca ccgggcgcga acatacccat
     4861 ctggcgctgc acaaagagga cgagaagatt cgctttcgcg atattcaggc gcagccgcgt
     4921 aaaattatct ccagccccac atggtccggt ctggaaagcg atcacgtatc ctataacgcg
     4981 ggatacacca acgttcatga gttaattccg tggcgcacgc tgtcgggacg ccagcagctc
     5041 tatcaggatc atccgtggat gcgtgctttt ggtgaaagcc tggtggctta tcgcccgcct
     5101 atcgacaccc gtagcgtcag tgagatgcgc cagataccgc caaacggctt ccaggaaaaa
     5161 gcacttaact tcctgacgcc gcaccagaaa tggggcattc actcaaccta cagtgaaaac
     5221 ctgctaatgc tgacgctctc tcgcggtgga ccgattgtct ggatcagcga aacagatgcc
     5281 cgagaactga ccattgtcga taacgactgg gtggaagtat ttaacgccaa cggcgcgctg
     5341 actgcccgcg cggtggtcag ccaacgtgta ccgccgggca tgaccatgat gtatcacgcc
     5401 caggaacgca ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg cggcattcat
     5461 aactcggtca cccgcgtttg cccgaaaccg acgcatatga ttggcggtta cgcgcagctg
     5521 gcctggggct ttaactacta cggcaccgtc ggttcgaacc gcgacgaatt catcatgatc
     5581 cgcaagatga agaacgttaa ctggctggat gatgaaggtc gcgatcaggt acaggaggcg
     5641 aaaaaatgaa aatacgttcg caagtcggca tggtgcttaa cctcgataaa tgtatcggct
     5701 gccatacctg ttcggtgacc tgtaacaacg tctggaccgg acgcgaaggc atggagtacg
     5761 catggtttaa caacgtcgaa accaaaccgg gcattggtta tccgaaaaac tgggaagatc
     5821 aggaagagtg gcaaggcggc tgggtccgcg atgtgaatgg caagatccgt ccgcgtctgg
     5881 gcagcaagat gggcgtaata accaaaatct tcgccaaccc ggtggtgccg cagcagattg
     5941 atgattacta cgaacctttc accttcgact acgaacattt gcatagcaca ccggaaggca
     6001 aacatatccc tactgctcgc ccgcgttcac tgattgacgg caaacggatg gacaaagtga
     6061 tctggggccc aaactgggaa gaactgctgg gcggcgagtt tgaaaaacgt gcccgcgacc
     6121 gcaacttcga ggccatgcaa aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt
     6181 acctgccgcg cctgtgcgaa cactgcctca atcccagctg cgtggcgacc tgcccaagcg
     6241 gcgctatcta caaacgcgaa gaagacggca ttgtgctgat tgatcaggat aaatgccgtg
     6301 gctggcgttt gtgcattagc ggttgtccgt acaaaaaaat ctacttcaac tggaaaagcg
     6361 gcaagtcaga aaaatgtatc ttctgttatc cgcgaattga gtcaggccaa cccaccgtgt
     6421 gctcagaaac ctgcgtgggt cgcatccggt atctgggcgt gctgctttac gacgccgacc
     6481 gcatcgagga agcgtcgagc accgagcgcg aagttgacct
//
LOCUS       DAZPAB010000198         6484 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZPAB010000198 DAZPAB010000000
VERSION     DAZPAB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.198"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            157..504
                     /locus_tag="ACHUPG_003477"
                     /pseudo
     CDS             157..504
                     /locus_tag="ACHUPG_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            557..952
                     /locus_tag="ACHUPG_003478"
     CDS             557..952
                     /locus_tag="ACHUPG_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR0862948.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="ACHUPG_003479"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="ACHUPG_003479"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR0862949.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="ACHUPG_003480"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="ACHUPG_003480"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR0862950.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="ACHUPG_003481"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="ACHUPG_003481"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR0862951.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="ACHUPG_003482"
     CDS             complement(5323..6423)
                     /locus_tag="ACHUPG_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR0862952.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
BASE COUNT         1476 a         1701 c         1544 g         1763 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctatttg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaat
//
LOCUS       DAZPAB010000199         6374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZPAB010000199 DAZPAB010000000
VERSION     DAZPAB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.199"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..378
                     /locus_tag="ACHUPG_003483"
     CDS             <1..378
                     /locus_tag="ACHUPG_003483"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862953.1"
                     /translation="IIRWLLKKRTNGSIRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACHUPG_003484"
     CDS             complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACHUPG_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR0862954.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACHUPG_003485"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACHUPG_003485"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR0862955.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="ACHUPG_003486"
     CDS             complement(1787..2476)
                     /locus_tag="ACHUPG_003486"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR0862956.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACHUPG_003487"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACHUPG_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR0862957.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACHUPG_003488"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACHUPG_003488"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR0862958.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACHUPG_003489"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACHUPG_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR0862959.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1621 a         1716 c         1577 g         1460 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagta tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcagaatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
     6241 atataacatc attgttttca atctgccatt tatgggattg accgttttct tttgacacgg
     6301 agttcaacaa tgggtaatgc ttccaactta ctgatttagt gtatgatggt gattttaagg
     6361 tgcttgcgtg gctt
//
LOCUS       DAZPAB010000200         6325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZPAB010000200 DAZPAB010000000
VERSION     DAZPAB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.200"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACHUPG_003490"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACHUPG_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR0862960.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACHUPG_003491"
     CDS             2219..2767
                     /locus_tag="ACHUPG_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR0862961.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACHUPG_003492"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACHUPG_003492"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR0862962.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACHUPG_003493"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="ACHUPG_003493"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR0862963.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACHUPG_003494"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="ACHUPG_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR0862964.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1596 a         1549 c         1605 g         1575 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggtctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgctgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agttttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaa
//
LOCUS       DAZPAB010000201         6292 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZPAB010000201 DAZPAB010000000
VERSION     DAZPAB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.201"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            28..858
                     /gene="rcnA"
                     /locus_tag="ACHUPG_003495"
                     /pseudo
     CDS             28..858
                     /gene="rcnA"
                     /locus_tag="ACHUPG_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            1077..1415
                     /gene="rcnB"
                     /locus_tag="ACHUPG_003496"
     CDS             1077..1415
                     /gene="rcnB"
                     /locus_tag="ACHUPG_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR0862965.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1497..2530)
                     /locus_tag="ACHUPG_003497"
                     /pseudo
     CDS             complement(1497..2530)
                     /locus_tag="ACHUPG_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(2546..5008)
                     /locus_tag="ACHUPG_003498"
     CDS             complement(2546..5008)
                     /locus_tag="ACHUPG_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR0862966.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            complement(5024..5698)
                     /locus_tag="ACHUPG_003499"
     CDS             complement(5024..5698)
                     /locus_tag="ACHUPG_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR0862967.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            complement(5779..6291)
                     /locus_tag="ACHUPG_003500"
     CDS             complement(5779..6291)
                     /locus_tag="ACHUPG_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFR0862968.1"
                     /translation="MIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFEAGGD
                     SVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGN
                     DKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQSGDVL
                     SSLVMRVAQD"
BASE COUNT         1697 a         1496 c         1254 g         1845 t
ORIGIN      
        1 gccgaattaa tactaagaat tattatcatg accgaattta caactcttct tcagcaagga
       61 aacgcctggt tcttcatccc cagcgccatc ttacttggtg cgcttcatgg cctggaacca
      121 gggcactcaa aaacgatgat ggcggcgttt atcatcgcca tcaaaggcac cattaaacag
      181 gcggtgatgc tcggactggc agcaaccatt tcgcataccg cggtggtctg gttgattgcc
      241 tttggcggga tggtgatcag taagcgcttt actgctcaat cagcagaacc gtggctccag
      301 ctgatttccg cagtgatcat tattggcacc gcgttctgga tgttctggcg tacctagcgc
      361 ggcgaacgca actggctgga gaatatgcac gggcatgatt atgagcatca tcaccatcat
      421 cacgatcacg aacatcacca cgaccaagga catcatcacc atcacgaaca tggcgagtat
      481 caggatgccc atgcacgagc ccatgccaat gacattaaac gacgctttga tggtagagag
      541 gtcatcaact ggcaaatttt gttatttggc ttaaccggtg gctttatccc ctgcccggca
      601 gcaattaccg tgctgttgat atgcattcag ttgaaagccc tgacactggg cgcaacactg
      661 gtcgtcagtt tcagcattgg cctggcgtta acgcttgtca ccgtaggcgt tggcgcagca
      721 atcagcgttc agcaggtcgc aaaacgctgg agcggattta acactctcgc taaacgcgcc
      781 ccctattttt ccagtctgtt gattggctta gtcggtgtgt atatgggcgt acatggcttc
      841 atgggcataa tgcgataact caatccttta ggcttgcatg ttatcttcag cccaggatga
      901 ttcttaaatc agctatttcc gctgactctc tgctcgctaa ggatgctttt agggcatcct
      961 ttcatttaca ctttttacga aatcatggga tcactaacaa aatatcgctt gtcagttata
     1021 ttgtatgaca ggaaagatat gcgactgata ttacagatcc ccaaagtgga gagtttatga
     1081 ccattaaaaa taagatgttg ctgggtgtgc ttttgctggt taccagtgcc gcctgggccg
     1141 caccagccac cgcgggttcg accaatacct cgggaatttc taagtatgag ttaagtagtt
     1201 tcattgctga ctttaagcat ttcaaaccag gtgataccgt cccagagatg taccgcaccg
     1261 atgagtacaa cattaagcag tggcagttac gtaacctgcc cgcgcctgat gccgggacgc
     1321 actggaccta tatgggtggc gcgtacgtgt tgatcagcga caccgacggt aaaatcatta
     1381 aagcctacga cggtgagatt ttttatcatc gctaaaaaaa gccccctcat catgaggggg
     1441 aaatgcagac accttgttat tttttattat tagccacttg ctcgttttgc ttgttattag
     1501 tcgtatttca cgttgattaa tgcggttgcc tccagtgcgc cagatttaac tttgtttgta
     1561 tcgtagacgt agtaactggc tgttatcgga attgaaaccg ttgtatctgt aagttttgac
     1621 tttgccgcaa tttcttttaa atttgtgtat cctttactgc tgttagtgat atttaaaagt
     1681 gtgactggcg aaccattact ttcaagaata aatcctacgc attatcttcc gcacctttca
     1741 gtattgtgtt gctgttggtt aacaagtcag aacttgataa atacacctgc attttccgtg
     1801 atgtttgatt ttgttgacct aataaatttt tacaacgcag aataatatcc ctttttttac
     1861 tatttattgt tgctttgtta cctgtggctg gtaattgtaa aacgggaata ctgtctaaag
     1921 taattaataa atcgtcctgt ttgcaggttg tggttatcgg gttgtatgac aatgtcagat
     1981 tgaaggaata gacacctgca ttttgtgcca atgcatttct gtagctattt acacatgcac
     2041 cccatgactt gccacctaat aaacacagta gaaattgtac tgcggcactc ccgcgtgtcg
     2101 atgatgaagc actggataat gtcgcaacac cattaatcat cactgaacca ccgccccccg
     2161 cagaaatatt cacttttgca ttactggccg caggtgtcac cgttaccgaa taaccactgg
     2221 tgaaactgct ctgattattt gacaacgtga tgttgttttt attggtccat tcaaaatgta
     2281 aatttaattt aatggtatcg ttataaagtc caacaataat atccgatggg ctcatagggg
     2341 tgatagtatt tgtactcacg catgacgaat cattaaaatt atgctcatat gtcgctattt
     2401 gtgtttgagt aagtgcaaaa ttcccctgaa caaccaaatt gtgctgaaca gataactcac
     2461 tataacatgc cgcatagctc acaggcataa ccatcatcat taatataaat agcattatgc
     2521 gaatttccat agaaccacct gactattact ggcaaatata atttctactt tcatcaatct
     2581 ctttaccgaa ggtgattgtg catgaaagtc cttgttgctt atcaattgct acattaaccg
     2641 atggcggtat ttcattggtg cgaataaata actggcttcc ctggccgaca acgccaatat
     2701 tatgaccatg gatatcattg acttcataac caaacgttaa tggttgacca tccgctctta
     2761 acgcttttat aaaccagggc ttgcgctgat cggtatcaaa attaaccagt acaaccgcgc
     2821 cgcgataagg ggcggcaatt ttccggttgc cacgtaattc tgcttcgcta tcgctttgcg
     2881 acacatccag catcaggtga ttttcccgat aaggtgtcat tccgtcgtat accaccactc
     2941 cattacggtt tgttgtgcga tatttttgcc cattgacata agcatcttta attcctggcg
     3001 cattcatcac agcaaacgtt tcggaaagac ggttcgccag attaacgcca cccgaccagg
     3061 cgacaatgcc ccctgaaaca ctggctccag tctgtcgata agtactcgac tgactataac
     3121 tgccattcac tgtcgcaacc ggcgcgttcc aggtcaaatt cgccccagct gtcgtttcat
     3181 ttccctgatg ttgatgactc aggttgacac cataattgaa ctgatcccga ctccctactg
     3241 ttcctgataa tcccgtatta tttgaggcaa aaccctgatc atcaaacgtc gttgagttag
     3301 acatatatat ttgccgacga ggcgtcgtaa cgtcatcacc ccaatcacag ggaattgata
     3361 taaaaatatt aaaacgtttc tcttcatgat gattctcgcc atacgcctgg cttgccgcga
     3421 gggtatagct tatccgccgc cagttattgg aataactcaa ctgataatcc ttactgctgc
     3481 cgctacgccc ccagtaatct cgccataacg tacttaagga cacagaaccc cagccttctg
     3541 gcaatgactg gctcatattg gcagaaaagc tatttttacg gccaaaatcg ttctggtaat
     3601 aatcggcaat gtcatagata tcgttttcat cacggcgata attatcttta ttgtttgccc
     3661 aaacgtgatc gttaaatgtc cggtaatcac gcgacgaata acgccaggcc gccagaccaa
     3721 aacgcgtcga cgtttggctc acaaatttgt tgtaggcaat ttgataactt tgcccgtcaa
     3781 acacatcgcc gttgtcttgt ttactatgcg acttcgtggc atcgacggaa attgcgccaa
     3841 tgcgcgtgtt ccaacccgtt ccgagggtaa acgcatagta attattagca accatcgtgc
     3901 cgccatacag cgtcaataag ttattaaaac catactgata acctgcctgg acaaaatcac
     3961 tttgcttgct cgcccccgcc gcaaaatcat atttcgacac gccgggttgc agcatatttg
     4021 gcaccgctgc ataaggcacc agataggtgg ttaccgagcc gtccgcctct ttcacgctaa
     4081 catcaagatc tgctccacca ccggccaact gcaaatctgt aatcgcaaac gggccaggag
     4141 gaacctcttt ctgataaacc acaaaaccat tctgttcaat agttaccagc gcgttactct
     4201 gagcaatccc ctgcacccgt ggcgtaaaat tttgtttcga gttaggcaac atctgcatat
     4261 cacgaaacaa ccgcacaccg ctgaagcgaa cagaatcaaa aatatcgctt gatgtgtaca
     4321 tatcacccac gcgaagcgtg ccgagaaatt gggcaaatcc acgttccaga tacagggtat
     4381 tgcttttcca cacccctgga ttgttatttg ttttactgaa actggcatca gaatgcagtt
     4441 gccactccag taaatttaac ccgctgttaa aacgtacata tgtactcttg ttgttacccg
     4501 acgctttata gtcgctgtaa tactgactca cataataaga agtataaaac gcattaatac
     4561 cccgctccca gttttccggt ggaacatagc cactttccag ttcttccacc caagcctgcg
     4621 ggacactgaa atcgagacga aaaaccccga tatcccagct atagctccca ccctgaacaa
     4681 gttgctcaaa tgttaaacat tgcttaccgc tggcgaagtt atcgctatta atgcctaacc
     4741 gcttgataac ttctcttgat aaacatgttt cttgcgggtt gtctttaaca ataatctcat
     4801 atttcccgcg ccattgctta ttgacataaa tatcgatgtc atactgcccc ggtaagggtt
     4861 gattatcatc aagacgaata tttgcaacct gctggtcttt cattccacct atcataaaat
     4921 gggtatcaaa ggtttcttca gctgcataag cttcaatgcc gagcaataac gctacgattg
     4981 ctgatgcaag tggggtcatt ctcaacatag gcagctcctg caattaaatt ttgtcactaa
     5041 tatagttgcc gtggtcattg ataatggtca gataccagtt atttgcatta ttacttttga
     5101 tattaacact ctgactttct aagggggcaa tcataatagt ttcataattg actttaacat
     5161 tattagcttt gacatccgaa atcgtcaccc aattagctga gtcatttttt atcaccaatc
     5221 cattgccact gcgatttacc agtaactttt taaaggtcgc tttatttacc ggagcaatac
     5281 ccgctggccg gtaaaacaac ttaattctgt tttgcatcgc aaacttcagt gcattcttgc
     5341 cttcttgctc tggactattt ggtggaatat acagaacatt cagataaaaa atgctttctt
     5401 tattagtggg cagtttattc ggcataattt tgatttttac ttgctgcccg gaatttgccc
     5461 ctatttttgc cactggtggc gttaacatga aaggaacctg aattttttct ggtggtaacg
     5521 acgtatcgcc atcatcaatc cacgcctgca gcagcgaaga acgatttccc tggttcatca
     5581 actgcaccat aacttcttta ttttctgccg ggtaaataat gcgcgtcccg tagataacaa
     5641 ttccggcatg tgcaggaagg accatagaaa aaatgagtaa agataataat cctttcatct
     5701 taatgcctct taaattaaaa agccgccatg gggatagcgg ccatgtttat tacctggaga
     5761 aacttgcaat tttatttatt aatcctgcgc cacacgcata accagagaag ataacacatc
     5821 acctgattgc actgagttat ctgtcagttt caggtatttt gctttaaacg gaatagcata
     5881 ttgaccttcc gctgttttat aactctcaat gttaaaaggt gcagaaacat ctaggacatc
     5941 gctgttatca cctacggtat cgttgctact ggctatcgcc ttatcgttac cggttatttt
     6001 gtcgtttgtg atcaacgaga gttttacacc agcaacttct ttcgggcagc taatttttaa
     6061 catgctgctg gttacaccga ctgcatcatc ataggtagaa tatttagctt taccttcaaa
     6121 gacagtggtt ggtacagtcg gcatattaat acttactgaa tcaccacccg cttcgaattt
     6181 acacggagat tctgcaatat ttcctttaat ggttaatgtt ccggtatcaa cgtctgcagc
     6241 aaatactcca gcgctcataa aaaaagaaga aaagacagcg gcagcaataa tt
//
LOCUS       DAZPAB010000202         6260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZPAB010000202 DAZPAB010000000
VERSION     DAZPAB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.202"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="ACHUPG_003501"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="ACHUPG_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR0862969.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="ACHUPG_003502"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="ACHUPG_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR0862970.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHUPG_003503"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHUPG_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..6054)
                     /locus_tag="ACHUPG_003504"
                     /pseudo
     CDS             complement(5797..6054)
                     /locus_tag="ACHUPG_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1663 a         1455 c         1471 g         1671 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt acaggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcaacagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg ccccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgttaa ttaacaaatg atatcttcta tatattttta
     6181 ttgtttatag atggctattt cggtaaaaat gcattttcag aatatcaacc agcggtaaat
     6241 cgaagtaaag ggagtgaatt
//
LOCUS       DAZPAB010000203         6258 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZPAB010000203 DAZPAB010000000
VERSION     DAZPAB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.203"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            85..735
                     /gene="csgD"
                     /locus_tag="ACHUPG_003505"
     CDS             85..735
                     /gene="csgD"
                     /locus_tag="ACHUPG_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFR0862971.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            740..1129
                     /gene="csgE"
                     /locus_tag="ACHUPG_003506"
     CDS             740..1129
                     /gene="csgE"
                     /locus_tag="ACHUPG_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFR0862972.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            1154..1570
                     /gene="csgF"
                     /locus_tag="ACHUPG_003507"
     CDS             1154..1570
                     /gene="csgF"
                     /locus_tag="ACHUPG_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFR0862973.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1597..2372
                     /locus_tag="ACHUPG_003508"
                     /pseudo
     CDS             1597..2372
                     /locus_tag="ACHUPG_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
     gene            complement(2436..2927)
                     /locus_tag="ACHUPG_003509"
     CDS             complement(2436..2927)
                     /locus_tag="ACHUPG_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR0862974.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3029..3583)
                     /gene="ycdY"
                     /locus_tag="ACHUPG_003510"
     CDS             complement(3029..3583)
                     /gene="ycdY"
                     /locus_tag="ACHUPG_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFR0862975.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3607..4344)
                     /gene="ycdX"
                     /locus_tag="ACHUPG_003511"
     CDS             complement(3607..4344)
                     /gene="ycdX"
                     /locus_tag="ACHUPG_003511"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFR0862976.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPCRLLNFLESRGMAPIAEFADL"
     gene            complement(4399..5337)
                     /gene="ghrA"
                     /locus_tag="ACHUPG_003512"
                     /pseudo
     CDS             complement(4399..5337)
                     /gene="ghrA"
                     /locus_tag="ACHUPG_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            5572..5659
                     /locus_tag="ACHUPG_003513"
     tRNA            5572..5659
                     /locus_tag="ACHUPG_003513"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5606..5608,aa:Ser,seq:gga)
     gene            complement(5809..6090)
                     /locus_tag="ACHUPG_003514"
                     /pseudo
     CDS             complement(5809..6090)
                     /locus_tag="ACHUPG_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
BASE COUNT         1678 a         1537 c         1431 g         1612 t
ORIGIN      
        1 gtgcaacatc tgtcagtact tctggtgctt ctattttaga ggcagctgtc aggtgtgcga
       61 tcaataaaaa agtggggttt catcatgttt aatgaagtcc atagtattca tggtcataca
      121 ttattgttga tcactaaacc ttctttgcag gcgacagctc tcttgcagca ccttaaacaa
      181 tcgctagcaa ttacaggaaa attacataat attcaacgtt ctctggacga tatctcttca
      241 ggctctatta ttcttctgga tatgatggaa gcggataaaa agcttatcca ttattggcag
      301 gatactttga gcaggaaaaa caacaatatc aaaatattgt tgctaaatac gcctgaagat
      361 tacccgtacc gcgacattga aaactggcct catatcaacg gcgtttttta tgccatggag
      421 gatcaagaac gtgttgtcaa tgggttgcaa ggcgtcctgc gtggcgaatg ctactttacg
      481 caaaagcttg ccagttacct gattacacat tctggtaact atcgttataa cagcacggaa
      541 tcagccctcc ttactcatcg ggaaaaagag atcctgaata agctgcgtat cggcgcgtct
      601 aataacgaga tcgctcgttc gttgttcatc agcgaaaata cggttaaaac gcatctttat
      661 aatcttttca agaagatagc cgtaaaaaac cggacacaag cggtttcctg ggcaaacgat
      721 aacctcaggc gataaagcca tgaaacgtta tttacgctgg attgtggcgg cagaatttct
      781 gttcgccgca gggaatcttc acgccgttga ggtagaagtc ccgggattgt taactgacca
      841 tactgtttca tctattggcc atgattttta ccgagccttt agtgataaat gggaaagtga
      901 ctatacgggt aacttaacga ttaatgaaag gcccagtgca cgatggggaa gctggatcac
      961 aataacggtc aatcaggatg ttattttcca gactttttta ttcccgttga aaagagactc
     1021 cgagaaaact gtcgtctttg cactgattca aactgaagaa gcactaaatc gtcgccagat
     1081 aaatcaggcg ttattaagta cggacgattt ggcgcatgat gaattctaaa taaaaaattg
     1141 ttcggaggct gcaatgcgtg tcaaacatgc agtagttcta ctcatgctta tttcgccatt
     1201 aagttgggct ggaaccatga ctttccagtt ccgtaatcca aactttggtg gtaacccaaa
     1261 taatggcgct tttttattaa atagcgctca ggcccaaaac tcttataaag atccgagcta
     1321 taacgatgac tttggtattg aaacaccctc agcgttagat aactttactc aggccatcca
     1381 gtcacaaatt ttaggtgggc tactgtcgaa tattaatacc ggtaaaccgg gccgcatggt
     1441 gaccaacgat tatattgttg atattgctaa ccgcgatggt caattgcagt tgaacgtgac
     1501 agatcgtaaa accggacaaa cctcgaccat ccaggtttcg ggtttacaaa ataactcaac
     1561 cgatttttaa gccccagctt cataaggaaa ataatcatgc agcgcttatt tcttttggtt
     1621 gccgtcatgt tactgagcgg atgcttaacc gccccgccta aagaagccgc cagaccgaca
     1681 ttaatgcctc gtgctcagag ctacaaagat ttgacccatc tgccagcgcc gacgggtaag
     1741 atctttgttt cggtatacaa cattcaggac gaaaccgggc aatttaaacc ctacccggca
     1801 agtaacttct ccactgctgt tccgcaaagc gccagggctt acaaaacctg cttaacgagc
     1861 gcaaggttat tcgtgcggca caagaaaacg gcacggttgc cattaataac cgaatcccgc
     1921 tgcaatcttt aacggcggca aatattatgg ttgaaggttc gattatcggt tatgaaagca
     1981 acgttaaatc gggcggtgtt ggggcaagat attttgggat cggtgcagac acgcaatacc
     2041 agctcgatca gattgccgtg aacctgcgtg tcgtcaatgt gagtaccggt gagatcctct
     2101 cctcggtgaa caccagtaag acgatacttt cctatgaagt tcaggccggg gttttccgct
     2161 ttattgacta ccagcgcttg ctggaagggg aagtgggtta cacctcaaac gaacccgtta
     2221 tgctgtgtct gatgtcggct atcgaaacag gggtcatttt cctgattaat gatggtatcg
     2281 accgtggtct gtgggatttg taaaataaag cagaacggca gaatgacatt ctggtgaaat
     2341 accgccatat gtcggttcca ccggaatcct gacagataaa aatgccggaa ttaataatcc
     2401 ggcatttact gtttaagcga aagcgtgagt gcccctcacg ctttattttt gatttcctgc
     2461 tcacggtgag ccgtctttgc actacgcgcc gccagccaca ggccgctgtt tttcattgcg
     2521 taaccaaata ccagcccaag cgccagcgaa ggtaacacca gtttccagtc accctgcccg
     2581 gcaaacgtcg cgcatgcgcc tataaacgtc cccggaacaa atgaaagcag cagctgtttg
     2641 gcctgaatac acatcagaaa cgcgacaata ccggttatga cataaccgag aatttccaga
     2701 tgtggtgcca gtgcactacc gtaaataatg accatcgccc acaccacgcc actaagcagg
     2761 gttgcagcgg agatcgccag ccctttcagc ccaccttgcg ggcaagcaaa gtaggccgta
     2821 cagcccagga agcccgccca gcttagtaag cccagggaaa cagccaccca tccccagata
     2881 ccggagagaa tgcctgttgt gattgcaata gagagaagta tgttcatgcc gcgcatgata
     2941 gcagaaaacg cggccatgaa tgagatcgag cacacatttt atgcaacaac aaattatctg
     3001 ttgcagttat gagtgacgat gatcacattt actcttcaga atcttcttcc agctcgtccc
     3061 acattgcact aatggcatca cgggttagcg gtgccatggt gcgccagaaa ggcgtggttg
     3121 catgggcctc cactttgccg aggaacgcac cacaccaggg taacagatac tcactgaaca
     3181 gtgtttccag tgcttcgctc tcatcttccg ttgactgatc ttccagccag gaagccgcaa
     3241 gcagcaatgt gccgatgtga tcggctggcg tatccgccag tagcatccct cgttcggaaa
     3301 gaaaagcgcg cacttccgct tccgttgcgt cctcaaccca tgcgctacga tatggcggca
     3361 cggcacattc atcgccgata aacaacgcat tgtaatcggc agagacttgc gtcatatcac
     3421 agcttttctg taaacgcgtc agtaactcat cctgctccag tggccagttc gcagccagtt
     3481 tcccctcacg aatcagagta aacagcggca ccagtaaagg atcttgcggt tggcggtaat
     3541 agagcgaacc cagcacacga cagaggatag aaaactcgtt cattaatata tttccattac
     3601 tagtaattaa aggtctgcaa attccgcaat cggtgccata ccgcgagatt caaggaagtt
     3661 cagtaagcgg cacggagaaa cattcaaaat gcgctctagc ggaaaatcta ccgcgtcgag
     3721 gattttaaga cactcttcaa attcccccat ggtaaacgcg gtgtgagaat ccgagcctaa
     3781 tgccacccaa cctccagcat cacgtaccgc tgcagctacc gcacgacagt tgtcttcact
     3841 gcccttacgt gagtgtaaaa atgaggaatt attgatttcc agcgccacct gatgtttcgc
     3901 ggctgcttca gcaacggctt taacatctat ttcatatttc ggatttccgg gatggcttat
     3961 tatatgcaca ttgccgctgg cgatagtgga gatcatcgct tgtgtattgg tcgctttgtc
     4021 atgtggcgca aaaaccggct catgaaaacc ggcaataatc agatccagcg agtcaaacat
     4081 tttaccgctg cagtcaattt caccatcaac atttttaatg ttagcttcaa tgccgcgcag
     4141 gatccctacc ccatcaacca ctcgcggcca gatacgcatg ttaatgaagt gccagtgatg
     4201 cggcgcatct tccatatccg ggccatgatc ggtgatcgca aaaagtttaa tgcccttttg
     4261 tttggcctgg gcaatgtaat cactcaatgt gctatatgca tgtgtgctgg caacggtatg
     4321 catatgaagg tcgacgggat acataactct ctcctgtatt caattcccgc caaggatagc
     4381 aggaatcctg atgctttatt agtagccacg tgcgcggtcg acttgcccgc agaccctctc
     4441 ccctttttcg agctgggcaa tagtgcgaga aatgtactcc acagcttcag cgggacgggt
     4501 aatcgcggcg acatgtggtg ttatcgtcac gcgtggatgt tgccagagcg gactttcagg
     4561 cggtaagggt tcacgattaa aaacatccag cattgcgcct ttaactttcc cgctatccag
     4621 cgccgcgagc aggtcatctt ccacaacatg aacaccacgc gccaggttga ggagatacgc
     4681 gccatcaggt aatttttcga gtaattgttg attaataatg ccgacggttt caggggtatt
     4741 cggtaacaaa ttaatcaata cccgacattg gctcagaaat gcagacagtt cttcccgtcc
     4801 ggcaaagctt tgcacgccag gccacgattt acgggttcga ctccagcaac gcagcggaaa
     4861 gcgctaggtt tgcagactct gagcaacttt acagcccagt acgcctgcgc ccaaaatgcc
     4921 gatggtaaaa tcttcccgat gatattcagg cagcggttgc caatgcgaac tattttgctg
     4981 gatgcgataa tcgtcaaaac gtcgaaacca atgcagcacc tgactgacag catattcctg
     5041 catttgctcg cccataccgg tatcttccag gcgaaaaagt ggaacagaag ggttcagcat
     5101 ttcagggtgt gcctgtagct tgctcaaaat agaatcaaca ccggccccga gtgcgaacac
     5161 cgctttaaga tcgcgccctg ccagcatttc aacaggagga tgccagacta aaacataatc
     5221 agcagagtca ttatctccgc ttttccatgc cctgactctt gcctgaggaa tagctttgcg
     5281 cagtgcctca atccaccatt gggtatcgaa cgttgggtga taaaagatga tatccatact
     5341 gactcccgaa aagcgttgtg cgaaatttat tcgcacttat cgttatgatc tacaaaggcc
     5401 accagcataa caaatccgtg gtcggtggca aaaaaagcag atttcgctta ttaaaaccac
     5461 acattgattg aatgtttgaa taaacgcgcg attttttcaa aaagtttgtt gacctcaggt
     5521 catgatttcc ctaaattagc gcccgttcca gcaagacagg aacgacaatt tggtgaggtg
     5581 tccgagtggc tgaaggagca cgcctggaaa gtgtgtatac ggcaacgtat cgggggttcg
     5641 aatccccccc tcaccgccag attataagtg catgttattg tttaacatgc gcttttttat
     5701 ttttccttcc agacagtatg ccatcgggta tgccatctga aaccggttta tatgccccgc
     5761 gatcatatct tatcggacaa tttccgatct ttgagcatac tggcaaagct atactgccag
     5821 catatcagga tagtgttttg cgataatatc attcagtctg tcaaccagtt ctggttttct
     5881 gaacgtgata tgcgcggagc ccttcttaaa gtatctgatg ctgaacatct catcttcata
     5941 gctctcttta ccctgaacag actgaatgtg atcatccaga cgaagggtga tgtttccccg
     6001 gttgtcgggg attggtttac cgctgaacag gtgcaacatt ctttccagat cggcgagtcg
     6061 gtcagcctgt tgtccggtga taagatgaaa tctatggtca cccccgtttt tgcaacactg
     6121 atttttgatt aatggttggc ttgcttaaat ctatccggcg tctctgtggg caaggatgcc
     6181 cgcgccccga tgagttccgc acctgataat cctaaaaatc ccctcggctt cagtgagcca
     6241 tttcatctgt taggtttt
//
LOCUS       DAZPAB010000204         6242 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZPAB010000204 DAZPAB010000000
VERSION     DAZPAB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.204"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..694
                     /locus_tag="ACHUPG_003515"
     CDS             <1..694
                     /locus_tag="ACHUPG_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR0862977.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1033..1803)
                     /locus_tag="ACHUPG_003516"
     CDS             complement(1033..1803)
                     /locus_tag="ACHUPG_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862978.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1848..2334)
                     /locus_tag="ACHUPG_003517"
                     /pseudo
     CDS             complement(1848..2334)
                     /locus_tag="ACHUPG_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(2294..2515)
                     /locus_tag="ACHUPG_003518"
     CDS             complement(2294..2515)
                     /locus_tag="ACHUPG_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862979.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2516..3199)
                     /locus_tag="ACHUPG_003519"
     CDS             complement(2516..3199)
                     /locus_tag="ACHUPG_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR0862980.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3263..3574)
                     /locus_tag="ACHUPG_003520"
     CDS             complement(3263..3574)
                     /locus_tag="ACHUPG_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862981.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3571..4473)
                     /locus_tag="ACHUPG_003521"
     CDS             complement(3571..4473)
                     /locus_tag="ACHUPG_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFR0862982.1"
                     /translation="MYFPSEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4913..5871
                     /locus_tag="ACHUPG_003522"
                     /pseudo
     CDS             4913..5871
                     /locus_tag="ACHUPG_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
     gene            5871..>6242
                     /locus_tag="ACHUPG_003523"
     CDS             5871..>6242
                     /locus_tag="ACHUPG_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR0862983.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRR"
BASE COUNT         1663 a         1665 c         1542 g         1372 t
ORIGIN      
        1 tgatgcagaa gccatatgtg aagcggcttc acgaccatca atgcgttttg tccagccaag
       61 aactgagacc cagcaagcaa taagggcatt acaccgggtc agagaatcgc tcatcagaga
      121 taaggtgaaa acaaccaacc agatccacgg tttcctgctg gaatttggaa tcagcctgcc
      181 gacaggggat gccgtaataa aacggttgtc tctcgtattg gctgaacacg aaatccccga
      241 atatctgagc cgccttttgg tgaggttgca tacccactac ctttaccttg ttgagcaaat
      301 agcagagctg gaatcagaat taagtcagtc aatcaacgct gatgataccg ctcagcgcat
      361 tatgacaatt cctggtgttg ggccgattac tgccagctta ctatcgtcgc aacttggtga
      421 cgggaaacag ttttcatgca gcagagattt tgcggcttcc acagggctcg ttccccggca
      481 gtatagcact ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg ataaaaattt
      541 acgccggtta ctggttcagt gtgcccgctc attcatgatg cagcttgaac gccagcatgg
      601 aaaacttgct gagtgggttc gggagcaact caacaagaag cattcgaatg tggttgcctg
      661 cgctctggca aataaactgg cccgaattgc ctaggcaatc actactcagc aaaaagaata
      721 tcaggcctga tatatagatg ctgacaaaac tcaattagct tattgcaaac aataagatac
      781 aagttaccgt ctggttttgc gaagattgat tattgatgac atgaacggca aatcgacctg
      841 attaaaaacc taacagggga aaatggctca ctgaagccga ggggattttt aggattatca
      901 ggtgcggaac tcatcgaggc gcgggcatcc cttgcccaca gagacgccgg atagatttaa
      961 gcaagcccac cattaatcaa aaatcagtgt tgcaaaaacg ggggtgacca tagattttcc
     1021 ccgccatcgt catcatttcc ggatttttgc cagggcatcc gccatgctgc ggatttcggc
     1081 ccgggcttcc cggacaactt cccgcagaac accacggtca aaccaccggc ggggcggttc
     1141 atccggtata cgcagcacat cgcaggccag cccactggac aggatatgct caatacaatc
     1201 gctgaccaga tacatttcca ccgccgaaaa acgtgcgccc cggttcagga tggcatcgag
     1261 caccacgggc aggttttcat cgtcttcatc gagccagcag gggtgatcgc tttccttcac
     1321 gaccaggcga aagcggcgcg tctggcgggt cagcaacgtc tgaccgcttg tggtcagcgc
     1381 cgcccggata cgccgcgcat cttctgcccc ccggcaactg acactgcata ctgcatcgcg
     1441 caccgcatca agaactgccc tgatttcgtc ttcggtcagc cattcaccgg catacgggaa
     1501 agacacctgc gttccgtccg ccggaagtac cggcagcgac tgccactgac tgccggacag
     1561 aacaagttcc gctccggttc cggtcagttt ctgaatggcg gaggccaggt gcggcggcag
     1621 cacgccatcg tggcggttaa cgagtaacgt cgccagccgg ggcgcatcca gccgcacgta
     1681 aatgctgatc cagcccggat agttcgggtt acattggtca acatccgtct cttccggcca
     1741 gccctgcgcc agcagcaggt tacgggcgtg acgacaaatc agagcattca gcgtttcatt
     1801 cataattcat gaactccctg tatcgccgcc cacaggcggc gataacatca gtcgttttcg
     1861 tgtgtgttgt ttttcagcag gctgcgggcc tgatcaacca tcgcctgtcg gcgggtaacg
     1921 tattccggga gggggaaacc gtgatcccgg gcagcccatt cacgtttcca cagtgcctgt
     1981 tcagcgatct gagtcagggt ctccagcagt tcagcaggga cggtcacctg gcggttttcg
     2041 cgcacgttgc tgataatctc catcgccgcg gcggaagaaa taccggactc aatccgctag
     2101 tcttccggga tgtcctccag gtgcgccagg acggtcctga tttcatgatc ggacagggaa
     2161 atatccattc cgtcagcgag ggcctgaatc tcttccgggg accagataac cagcatcagc
     2221 ggcgtgtctc ccgaatactt tgctgccagc gcccggcata gcgtttcgca tgttccgtac
     2281 atactctctc ccctcaatct ttgaagcagg aatccagttc cagaatttcg gtatatgcct
     2341 ccatcaagag gcggtttgcg cgaaacaacg tctgacgcac cagacgttcc ggcagggtgt
     2401 cagcattaaa gctgtaccgc cgctccatct cattcatggt tgtacgcagg tcgcacatgg
     2461 cattggccac ctccgccact tcggcgcgaa gtttttcgtg tccggcatag ttcatttacg
     2521 cagcctccgg cataaaccag tcatcattcg cggccccctg ctgcatggcc cgctgtacgg
     2581 cagccagtcg ttgctgtccg gcacggtgat actgctcaag cagctcgata ccgatatacc
     2641 ggcgtccgga ctggagggcg gcgacgcagg ttgaaccgct gcctgcaaac gggtccagca
     2701 ctatcgcatt cgggtgtgtg aagctctcaa tcagcggttg caggctggta acgggctttt
     2761 ccgtcgggtg atggcggttg cccgaatatt tccagcccaa cacgtccggc agcgggtttt
     2821 gtggcagacg tggacggcct tttgccagga tgtaggcgca ttcgtggcga tagcccacat
     2881 atgcggcctt cgatgtgtag tttttggtga agaccaggtg accaacaacg ctgaatcccg
     2941 catttttcca ggcagacata aagcgatcga cgcggttcca gccatagaag ctcaccatca
     3001 gcgcgtcttt tttgagtacg cggtacattt cattacaggc cggttgcagc cattcatcag
     3061 ttttatcacc agcaatggta cgcccctgac ggtcacgaaa accgacgaga tatggcggat
     3121 cggtgaggat gaaatcaatg gcgttatcag gaatacgggc cataacatcg atgcagttac
     3181 cgaggacaaa acgggacata acagatcctt ctcttacaga gggcacccct gtggtgcccg
     3241 ttccccgctg cctgtgggct gttcatcgct tccggtaacg ggtcaggccg accgtggaat
     3301 accgcagccg tcgtcttttt gacagcaagg ttatgccgcg aaagcggctt gaagggcgtt
     3361 catcagaaaa gggcgacggg ctggcgtaca ctgtcaccga caccagccgg gagcggtaaa
     3421 agcgcccgtc ccgtgcccgc cggacgcggg acactgacca caggcagcgt ttatgccgcg
     3481 ccgcgtcagc cggcgcagta aaaagaggag cacggcgaca tcctttgtcg gaaaagaaac
     3541 gggcggttgc ccgcccgtct ccgtcatact tcatccgcca taatgcgcca cattgtcgac
     3601 tatccacgca ggtccggtga cttccggctg ctcatcgtca tctgtcgggg aagaccattc
     3661 aagttccccc cacagcacat tgacgagctc tccacgctca tacaactgcc agccacagaa
     3721 atcacagccc tgttcggcat accagtgctc cagcgtgcag ctgaaacggc ggcttaattc
     3781 tgcaataacg tcgctttccg gctgacacca cggagtgtcg aaatccacct gaatgaagtt
     3841 ttctccctga ctgctgatat tcaggtcata tccacaaggc cacttcacgc cataccgttc
     3901 cgtataccag tgatatgcag aaggaacacc gttcagaaca ccaccattaa agccgttcac
     3961 ttcaacatcc aggcgggtcg gcagaaccgt cagcaggtca aacggcatgg ttttttccgg
     4021 cactacgttg tcacacagac ggttccacca caccgataca tcctcgttac cccatatgtc
     4081 acaccagtca cctctttttg cggtgaaatg cacagtgatg gtttccctgg catcatccgg
     4141 taatccttcc caacgacgct ggccagtacc gctctgtaac cagagttcat gcagcatcag
     4201 gcagttttgt tcatccagca acacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc
     4261 gatattttcc gtcgatacag caccacgtcc ggcagccgtc actccggggc actgttcaga
     4321 gcggatattt tcagtgatct gcagaagtcc ggcacttccg gccagaaaca gctgaatacc
     4381 ttcatttgtg gcgcggcgat aaagcggtgt gactgcaccg ctggccagtc gtttaatctc
     4441 agcgatctgt gccggctcac taggaaaata catacgattc gagcaccagt ttggcatggg
     4501 aattctcctc tcacatgcgg cctccatcag gccgtgccgc acctgtaacc gggcgaccgc
     4561 atacgaatgc aggtgcggcc ccgaagccgc agcgcgtgga ggaggaatga cgaaacgtga
     4621 ccgggtgagg aggccggcga cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc
     4681 cggcacggcc tggttaaata gtatccgtct cagttcgtgg cgcaaaatga gaaaccaata
     4741 agaactcaaa atgaaccaca acaagaacat aaagagactc taaaagagca acaccgtttg
     4801 acatagactc aaaatgagtc acaataagaa acaataaaaa actcaaaaag atgcaccgac
     4861 ataaaggatg ctcaatttga gataaacaca aactcaaaac gaggcaaaaa gaatgctaaa
     4921 agtatcatgc gatgatggtt caactaacgt aaaactggcc tggcttgaag atggcgaggt
     4981 aagaaccagt ctgtcaggaa acagctttaa agaaggctgg aacccgggac tgtttaatgc
     5041 cggcaaagtt tacaattacg tcgtggatga aaaaaataca cctatgattt gggaagcact
     5101 gctgtcatag gtacaacaca tgtcagttat caatacagca ccaccaatct gcttgccata
     5161 catcatgcac tgctgacatc cggccttcag ccacaggatg tagaactgac agtaacatta
     5221 cctgtgaccg aattctttga taatgacaat caaccaaatg aagaaagaat agaaagaaaa
     5281 aaggcaaatg ttctccgtga gatatctctg aataaagggg aaacatttaa aattaaaaaa
     5341 gttaatgtaa tgcctgaatc tttgccagct gcatttgaat cactaaaaaa agataaagta
     5401 aacaaacttg aacgcagcct gattatcgat cttggaggaa ccactctgga ttgtggttta
     5461 attctcggtg catttgaagg catttctgaa atccgtggct attcagaaat cgggacatcc
     5521 cgcatcactc ataccgtcat gaatgcgctg acgaaagcct ccacaccatg taattatttt
     5581 attgctgatg agttaataaa aaacagacac gataatgaat atctccagac attaataaat
     5641 gatgtagctg aaataaaaaa catttctcat gtaatagaca gagaagtgaa atctctggca
     5701 gaaagcatca ggcaggaaat ttcaacattc tcaggaatga accgaatcta tctcactggc
     5761 ggaggagccg agcttattta ccctcacata aagcaatatt tcccgaatct gaaagtgaac
     5821 aaggtggatg aacctcagtt tgcattggtg aaagcaatgg tgcatgcgta atggaaagca
     5881 gtgatccaaa aaaaagaaag aaagttgtgg catacctgca cccggcactt tatccgcagg
     5941 acaacctgac acaacaaaca atagactctc tcccagtaca aatgcgggga gatttctaca
     6001 gacaatccct tatttgcgga gcagctcttt attctgttgc cccccgactg ttaacgctaa
     6061 tcagcgtttt tttcagtgag aaaatcactg cagaaaatct ggtgaaactt atagaacaaa
     6121 caacaggata cacatccaca tcaattgata taagtgtgtt aaaaaacatc atagaggcat
     6181 cttcagaaaa caaatcagag agcatcactt caaaagatga tttcgaagaa caaaccagac
     6241 gc
//
LOCUS       DAZPAB010000205         6224 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZPAB010000205 DAZPAB010000000
VERSION     DAZPAB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.205"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..1007)
                     /locus_tag="ACHUPG_003524"
     CDS             complement(<1..1007)
                     /locus_tag="ACHUPG_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862984.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     G"
     gene            1108..1395
                     /locus_tag="ACHUPG_003525"
     CDS             1108..1395
                     /locus_tag="ACHUPG_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0862985.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            1367..2896
                     /locus_tag="ACHUPG_003526"
     CDS             1367..2896
                     /locus_tag="ACHUPG_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFR0862986.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(3066..4283)
                     /locus_tag="ACHUPG_003527"
     CDS             complement(3066..4283)
                     /locus_tag="ACHUPG_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0862987.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(4650..5636)
                     /gene="ycdU"
                     /locus_tag="ACHUPG_003528"
     CDS             complement(4650..5636)
                     /gene="ycdU"
                     /locus_tag="ACHUPG_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFR0862988.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(5633..6161)
                     /locus_tag="ACHUPG_003529"
                     /pseudo
     CDS             complement(5633..6161)
                     /locus_tag="ACHUPG_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         1908 a         1460 c         1213 g         1643 t
ORIGIN      
        1 caaccattct tatcattaat attaaataca agatctttca gacgggaaat atattcctga
       61 gatttatcat caagtgaaac actataacga gacaatgttc cttctgcact gttttgaaac
      121 ttataaaaca tttccagtaa tttttttgca taaggcgttg tatcaaagct ggtaaagtca
      181 taaagatatt gcgcagcgta accatacaaa ccatgaatcc agagtccttc tctcaccaac
      241 actctgagat aatatgcaag gctttgaagt atcttcgtct ttcgttgtat atcaagtaag
      301 acacttaatg aataaaatgc attatagaat ttatcaatat tcctgagcac tttctccagt
      361 tcatccttat ccagcttctt ctcccggtaa tcctttttat attcattttt gatagccatg
      421 atatattgtt gcatcaggct cttgcttttc ccgttatcct tccgtatagt tcctgtatat
      481 ttccccagag catacagctt tccattttca tgaaaatctt ccttgttgaa tcccagttca
      541 gccagatatt cccgaagata acgtttaaca gcagcttcat cgctttctgt atcagcgtga
      601 tcaagacaag gacaatacga tttctgtaaa aaattataaa tatgataata aaaagcagag
      661 tcattctctg gtctgtatgt attatacata tctacaacaa gaaaaataac cccaaaagtg
      721 cgcagacaat agctgcgaag gaattcaggt tttagtgtgg aatgcactgg ataagaattc
      781 atcatgtaat aaaaatgttc atatcgggca tatatgatcc gccgttgctc ctgactgaac
      841 accccttcag catcaataat acgactgatc tcattataac gttgatccca gaatttttga
      901 tgcatttcaa aattggtact cttccgcttg tgtgccggat tctcgccttc cttaaacaac
      961 cccaaaacag tacaaatatc atacaacata tttgtaaatg tgcgcatatg ctgtgctctc
     1021 cggttggttt gcattttatc gcagaggaca ttctgagctt aacgtatcaa actgacacaa
     1081 cgacatgcaa tgcatatgga gaacaatatg tcaaatcaaa ccggacagct ttacacgcct
     1141 tcacgcatcc tgcgtatggc tgaacttacc cgactgctgg gtatatcaag atctactatt
     1201 tacgtgaaaa taaatccggc ctcgaaatac cacgacccta cctttcccaa accaataagg
     1261 cttggtagtg catccgtagg ctggcgggca tctgccatcg atgagtggct gatgttacat
     1321 acggcaccag cctggtctga acctgaaaac aaactaaagg aacttaatga tgacgacaaa
     1381 actatcactc cctgaacttg acacaactaa actgcctgac aggtgccagg cattacttga
     1441 tgaaatgcat gaagaaacag gaatacgccg tgaaatactt ctgtctgtta tgctgacggt
     1501 catggctgcc tcggtccagg atacgcatga agttgaactt tccggagggc aacgtaccag
     1561 ccttcagata tttatgtgtc tttcatcagc atcaggcagt ggaaaaacct ctgcctgcgc
     1621 aaaattaatc gctcctgttc atgaaacaga agaagaacta catcaggcat atatcgacga
     1681 taaaaaaaac tatgaccgaa tgatggaaat gtggacaaca gataaaaaaa tcctggagcg
     1741 gagatataaa aaggaaattg aaagatcccc ggaaaatgct gctgctgcac gagcagcact
     1801 tgaaaagtgt atagcaaata aacccgaacc tcccgtacaa caggttctca tcgtgaatga
     1861 cgcgacacca gaagggatag ctttgaaact cagccagtca ccctctcttc tgttgctgtc
     1921 tgatgaagga ggtactattc tggataagcg tttcgaacgc aaatcggcac tgtacaacac
     1981 attatggagt gggcaaccag tcaacgttga acgggcatcc agacccgggt tccggctgaa
     2041 tgatgtccgt ctcactatac ttatactgac tcaaccggta atatttaata agttttttac
     2101 tctcactggc gatcagatcc ggggcaatgg ctttctggcc cggttgctat tttgcgaacc
     2161 cggagcaatt aaaataatga cgacagaacc gcatactgcc actcctgttg tgacgcaaca
     2221 atgtgcaagc tgttttcgta agaaaagttt tggcagtcag atcagagact ctctgagagc
     2281 atccagagaa agacgtgcaa aaagtaaaga gcgcatctgc atgacattat caaatgcagc
     2341 atcccgcgca ctggagcgct ttcacaaaga aaatatggat actgtcagac agaatcctca
     2401 tatgggtaca tttgaagaca ttattgtcag aaaaagagag caggttgtca ggatagcagc
     2461 acttctggaa ctggagaaag atccagactc gacagtaata acgctggaaa gtactaatag
     2521 cgccatttat ctcattgaat tttatttcaa gcatcttata tataaacttg aatcccttcg
     2581 ggaaatatcc ccggctgaga aacttgataa atggcttcag aaaagaatta tcacaacagc
     2641 aggttacata ttccagaaaa gttatatttt acaatatgcc ccatatgctc tcaggaaaaa
     2701 atgtgttctg gatgaagcac ttgacatact ggcggaacaa aggaaaataa ggattgatga
     2761 taacctggtt gtttatatag gaaacacgat tacaccatcc gaactggcaa aaaaactaaa
     2821 tattcctgca ttcgatgccg gagttttcat ttgtgatcat caaaacatac tgaagtatca
     2881 ccgaaacaga ctatagatac tatcggacaa aaaataaatt atcacaagtg tattattcgt
     2941 tataataatg aataatcgtt atctgagtgc aatgccaggt tttcattacg aactggcatt
     3001 gcatttattt ttcaaaaaag aaagcagtaa ccatttggct actctgagta tgccagtcca
     3061 tgaaactaag cgaattcctt agtgttttta gcaagttgag acatcacata atccgcccat
     3121 gcctgcatca tcggtacacg ctgctccagt agatcagtac gatgatatgc cgcctcaacc
     3181 ttatttttca gcgtatgagc gagcgccctt tccgccagat cccgcgaata cccctgttca
     3241 ctacaccagt ccctgaatgt tgagcgaaaa ccatgtgccg tggcaactcg ccccggaatg
     3301 tcactgacgg ctttcttttt acgcagaaaa cttgtcaaca ccatatcgga aaggatctgc
     3361 tgctttctgg gtgaagggaa caccagttca tcatgcaggc cacgtatatt ttccagaatg
     3421 taaatagcct gccgggataa aggaacacga tgctgtagcc tagctttcat tctttctgca
     3481 ggtatagtcc atacccgctt atgaaaatca atttcagccc agcgcattcc cctggcttcg
     3541 cccgagcgag ttgctgtaag tatcaccatt aataacagtg cgcgggtaac attataaggt
     3601 tcatcactat acacactggt cgccacaaaa agcggtaact gcctccaggg cattgcgggt
     3661 tggtgttcat cacgtcctct tgtctgctga ggaagcaaat ggtcaaccac atcaacagga
     3721 tttgccacac aaaaaccgtg cgcccatccc cactgcataa caacatgaat gcgctgttta
     3781 acccggcttg ccgtttctga caaggttaac cagactggac gcagtgtttc tgccacgtcc
     3841 gcagccgtaa tcgaatccag cgtttttgct cccagttgag gaaacgcgta attctcaagc
     3901 gtcgataacc actgccttac atgctttgga ttctcccatc caggagacag ttctgcatgt
     3961 acacgcctgg ctgcatcggc aaatgttggg ataacgactt tctcagattc agccttttta
     4021 atctccagag gatcatcacc tgcagcaagt tgctctcgca ttatccgggc agtacgtgca
     4081 gcttcagcaa tactgacctc tgggtaagtt cccaatccag cattacgtct tttttgtgtc
     4141 accggactta cataacgaaa aacccatttc ccccgcccct ttactgaaga aggatgaagg
     4201 gtcagtccgg taattccccc atggggcaat ggtttgtcat caggtttgat atgtcttgct
     4261 ttcgtatccg tcaatactgc catacgctaa ttcccgtcac tctggtatgc catccagtat
     4321 gccattagtc cctcgcttca ctaagaatac attagataac atcggacaac aaaaagtgta
     4381 atacattgtt tataaagaaa ttatataaca cccctggatt aagtcagatt tatttcaggc
     4441 ggtccccctc accgccatat ttaaagaaga gcgcgtacga aagtacgggc ttttttttcg
     4501 tatattgtac acaccgggtg cccccgccca cgtctgttag ggcgagggga aattgtgcac
     4561 atcaactgtc tggttactca gtcaacaatc aaaatgcgta agatagtaca gaacatatat
     4621 gcgttatctg gtcacttcct gtaacttcct cacttattaa aatgccccca acggaaatac
     4681 atgtcacatg ccatcataaa atcgttatgc cagaggaatc gacaatggaa aaaatagtaa
     4741 attaagcacg gtacatagca gagtaaaaaa taggcttcaa accaaatccg ccattccaca
     4801 agcgttgcca ataccgacac cagcgtaatc cacatcattt caataacccg caatatgaat
     4861 aaccgaagcc aaagcggctt ggcaaaaaag gtcgacaacg tattttcact ggagaaaaac
     4921 agatcgattt cctgcaaaaa aaacctgccc agaaaagcgg gccgcttgga gaagcgcaga
     4981 aaaaacagat taatcgccaa cactacgcca caaagaatga gcaatgcaat tatcgattta
     5041 aacgttaacc actgcccctc tgtcacaaaa gaatcactgt tacgcagcgg gctgatagta
     5101 agcaggttaa caaccgttga gatgcacacc gaaagcagca catagcgagt cagactttta
     5161 tattttactt ttttcgctac tgaaatagcg acctgcttga cataactggt gcggatcatc
     5221 cgaaacagca gaaagatgac caacggccca agtaacgaaa tcaggaacag cgcatataac
     5281 gagggtagaa gctggtatag cccccaactc attgcactca acatgattaa cgacagaaat
     5341 cctgccacca gaggttgtgt aagaaataat tctattttgt ggttctgctt tttcttaaac
     5401 cacgaaaaat cgatattctt cgtgacgcat ttataagccc agtacgcctt gagatcaaaa
     5461 ataaaattat acagcaccag ggccagaatc ccactaacta caccaatatc ctctgatgga
     5521 aaagtaaacc ctgcattttt ccaataaaag cctatcagaa taagtccaat agcataaatg
     5581 tatatcagat ttcgtttatc ctgaaagcga ataaaagtta aataatcagg aatcatttga
     5641 atacctttgc atcactaaat gctttacgac aatgcataac tcgttgctga tccacgccag
     5701 caaaatcatt taacatattc cccaaatagg gcttattact tatttcaagg aaagctaccg
     5761 gggttggcat attcagaata tgtatcgaga gattcagtcc atttcaccgt atgagttaag
     5821 tgtaatgaga gattttcttt gataattgac ggagcgacct cgctttttgc tgttacgttc
     5881 atcagaacct gatgctctgg cgaagcgata tccagtccgg caagataatc gcgcattgcc
     5941 tgaacgccgt cttccatcaa acgcgtgtgc caggcaccgc tcacaccgag tttaaccggt
     6001 tcgtatccag cggccatcag cagcgtggta aattcattca acgcagcctg cgttccccca
     6061 ataacctgct ggcgcggcgt gttatcacag ctaatatcca gcgcaatgcc tgattccgta
     6121 atcatcgtct gcagttgttc gcggttgatg cctttaaccg cctgatctta cccagcaata
     6181 gtggacacgc ggctaagtga gtaaactctc agtcagaggt gact
//
LOCUS       DAZPAB010000206         6161 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZPAB010000206 DAZPAB010000000
VERSION     DAZPAB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.206"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..430
                     /locus_tag="ACHUPG_003530"
     CDS             <1..430
                     /locus_tag="ACHUPG_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0862989.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
     gene            550..723
                     /locus_tag="ACHUPG_003531"
     CDS             550..723
                     /locus_tag="ACHUPG_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFR0862990.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            823..1644
                     /locus_tag="ACHUPG_003532"
     CDS             823..1644
                     /locus_tag="ACHUPG_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFR0862991.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            1986..2459
                     /locus_tag="ACHUPG_003533"
     CDS             1986..2459
                     /locus_tag="ACHUPG_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR0862992.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            2475..2951
                     /locus_tag="ACHUPG_003534"
     CDS             2475..2951
                     /locus_tag="ACHUPG_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFR0862993.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            3014..3235
                     /locus_tag="ACHUPG_003535"
     CDS             3014..3235
                     /locus_tag="ACHUPG_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFR0862994.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            3398..3772
                     /locus_tag="ACHUPG_003536"
     CDS             3398..3772
                     /locus_tag="ACHUPG_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFR0862995.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            3819..4193
                     /locus_tag="ACHUPG_003537"
     CDS             3819..4193
                     /locus_tag="ACHUPG_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFR0862996.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            4190..4681
                     /locus_tag="ACHUPG_003538"
     CDS             4190..4681
                     /locus_tag="ACHUPG_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFR0862997.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            4693..4890
                     /locus_tag="ACHUPG_003539"
     CDS             4693..4890
                     /locus_tag="ACHUPG_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFR0862998.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            4975..5535
                     /locus_tag="ACHUPG_003540"
                     /pseudo
     CDS             4975..5535
                     /locus_tag="ACHUPG_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            5607..>6161
                     /locus_tag="ACHUPG_003541"
     CDS             5607..>6161
                     /locus_tag="ACHUPG_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HFR0862999.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHY"
BASE COUNT         1511 a         1655 c         1551 g         1444 t
ORIGIN      
        1 aagtcctgac acaaacgccg ccactgtaaa tcagacactt aaatgcgact caaaagcccg
       61 ggctgaccag cctggtgcat gtccaacaac gtacgagtta tacgaaggtg acgctaccta
      121 cagagctgct atcgacaaag cattaaaccc tgttgggtta aacggaatgt ttggggaaga
      181 cggctatatg gatggtcctg atggtggtgc gtatcctgta aacattaacg gaacgacctg
      241 ggtagaaggt ggcggttgta aagcccacgc ctgcggctgg gactatatcg taacactcta
      301 taacccaaaa acgcataaag tcgttggtta ctattacaac atcgaccctg gttacctgat
      361 ttggtttggt gaaactggcg ttcacgaatt cgcgtacctg gtcagggact acgtaaacaa
      421 aacaaattaa aggcgtaagt tatgcggttc atcttcgcgt tattaattta ttgatatcaa
      481 aagtaaatac ggctctgcca tgatgacagg gacttatatt ttcagggaac caaatacagg
      541 tctggttgta tgtcacgttt attgcccgaa gtccctttct cacgcgagca ggctgttgtc
      601 atcacaacag cttaccgcaa tgtgtttatc gacgatgacc agggaactca tttccgtcgg
      661 gttatccgca acgcagagac tgacgccgga aggttcgcca gtgacggtct tctcagacaa
      721 taaacctcat taatttcgtc catcaggccg cgtcttctcc gggagacgtg gctttttcat
      781 ttattacacc agtaaatctt aaccaccgat aaggagcaaa ttatgcgact agccagtcgt
      841 tttggttatg ctgcaaacca gatacgccgt gaccgtccgc tgacacacga agaactgatg
      901 cactatgttc ccagtatttt cggtgaagac cggcatactt cccgcagtaa acgctatgcg
      961 tacactccca ccatcaccgt actggaaagc ctgcagcggg aaggctttca gccattcttc
     1021 gcctgccaga cacgtgtgcg tgacccgggt cgccgggaat acacaaaaca catgctgcgt
     1081 ctgcggcggg ccggacagat aaccggtcag catgtccctg aaattattct gctcaactct
     1141 catgacggtt catccagcta ccagatgtta cccggatatt ttcgtgccat ttgtaccaat
     1201 ggactggtct gcggtcagtc tctgggagaa ttgcgtgttc cacaccgggg aaatgtggtg
     1261 gagaaagtta ttgaaggggc ttacgaggtg gtgggcgtgt ttgaccggat agaggagaag
     1321 cgtgatgcca tgcagtcgct ggtcctgccg ccaccggcac gccaggcgct ggcacaggcg
     1381 gcactgactt accgttatgg tgacgaacat cagccagtca ccaccgctga cattctgaca
     1441 ccacgacgca gggaggatta cggtaaagac ctgtggagcg catatcagac catccaggag
     1501 aatatgctga aaggcgggat ttccggccgc agtgcaaaag gaaaacgtat ccacacccgt
     1561 gccattcaca acatcgacac cgatattaag ctcaaccgcg cattgtgggt aatggcagaa
     1621 acgctgctgg agagcctgcg ctgaggcagc atgtccctga aggcgtgatt caggcaccag
     1681 aatcacccga cgccccggag gcgctgccgg ggtgtcgttc catttctgat gatgacctgt
     1741 gcgcctggtg tacccgtctg ctttaccgtc ccggagaaat cagtctgtgc agactgagta
     1801 tacaggcaat ggcatctggc catccgtctg tgaccggaac ggttacgcgt acggctgtcc
     1861 ggaatttcag ccgaatatca ttcatccctg atattaccgg gtcatcacgg tcagtgatga
     1921 acgtgatgtt gccagctccc gacctttact ccctctcatt tattcacatt acaaggattt
     1981 catacatgaa aacattgtct cagaacacca ccgcttcggc ctgtgcaccg gagactggcc
     2041 tgcaacaact ggttgccact cccgtccctg atgaacagcg catcagcttc tggccgcagc
     2101 attttggcct cattccacag tgggtcaccc tggaaccccg tgtcttcggc tggatggacc
     2161 gtctgtgtga agactactgc ggtggtatct ggaatctgta caccctgaac aacggcgggg
     2221 catttatggc acccgaaccg gatgacgatg atgacgaaac atgggtactg ttcaatgcca
     2281 tgaacggtaa ccgcgctgaa atgagcccgg aagccgccgg cattgctgcc tgtctgatga
     2341 cgtacagtca tcatgcctgt cgtacggagt gttatgccat gacggtccat tattaccggc
     2401 tgcgggatta cgccctgcag catccggaat gcagcgccat tatgcgcatc atcgactgaa
     2461 tggaggcagg gacaatgcag cagctttcct ttctgcccgg aaacatgacg cccggcgagc
     2521 gcagtctcat tctgcgggcc ctgcaaaccc tggaccgcca tcttcatgaa cccggcgtgg
     2581 ccttcacctc cacccgtgcg gcacgggaat ggctgattct gaacatggcg ggactggagc
     2641 gggaagagtt ccgggtgctg tatctgaaca accagaatca gctgattgcc ggtgaaaccc
     2701 tcttcaccgg caccatcaac cgtacggaag ttcatccccg ggaagtgatt aaacgcgccc
     2761 tgtaccacaa tgccgctgcc gtggtgctgg cgcacaatca cccgtccggt gaagtcacac
     2821 ccagtaaggc agaccggctt atcaccgaac gtctggtaca ggcactgggc ctggtggata
     2881 tccgggtgcc ggaccatctg atagtcggtg gcaaccaggt tttctccttt gccgaacatg
     2941 gtctgcttta acccgtcaca accacatcac acctgttttc acttttatct tctgtcttca
     3001 gaggtatcac atcatgagaa tcatcacccg tggtgaagcc atgcgtattc accaacagca
     3061 tcctgcatcc cgtctttttc cgttctgtac cggtaagtat cgctggcacg gcagtgctga
     3121 agcgtatact ggtcgtgaag tgcaggatat tcccggcgtt cttgccgtgt ttgcagaacg
     3181 ccgtaaggac agttttggcc cgtatgtccg gctgatgagt gtcaccctga actgatgttc
     3241 cgtcacccgg gaaacgagta cgaccagatg acctcagacg ccgctgtgga tgaggccatt
     3301 ctcatcaatg catacatctt caccgaaacc ggtcagcgcg ccggctgacc tgtcatcgtt
     3361 aaaccattct taaccacttt tcagagagga ttttatcgtg tcagacaaac tctccgggat
     3421 aactcatccc gatgacaatc acgaccgccc ctggtggggg cttccctgca cagtgaggcc
     3481 ctgttttggc gctcgtctgg tgcaagaggg taaccgcctg tattaccttg ccgaccgcgc
     3541 cggtatcaga ggccgcttca gcgacgcaga tgcataccat ctggaccagg cctttccgct
     3601 gctgatgaaa caactggaac tcatgctcac cagcggtgaa ctgaatcccc gatatcagca
     3661 taccgtcacg ctgtatgcaa aagggctgac ctgcgaagcc gacaccctcg gctcctgtgg
     3721 ttacgtttat ctggctgttt atccgacacc agcacccgca accacctcat aaccccgaag
     3781 ctctcacctt tagcactacc agttacagag aacttaagat gaaaacatta tccgacacac
     3841 atgtacggga ggtatctcgc tgcccgtctc ccgtcaccat ctggcagaca ctgctcatcc
     3901 gactgctgga ccagcattat ggcctcacac tgaatgacac accgtttgtc gatgaacgtg
     3961 tgattgagca gcatattgag gcaggcattt cactgtgtga tgcagttaat tttctcgtgg
     4021 aaaaatacgc gctggtgcgt accgaccagc cgggattcag cacctgtccc cgctctcagt
     4081 taataaacag tattgatatc ctccgggctc gccgggcgac cggcctgatg acccgcgaca
     4141 attacagaac ggtaaataac attaccctgg gtaagtatcc ggaggcgaaa tgatgaaact
     4201 ggccctgaca ctggaagccg acagcgttaa cgtacaggca ctgaacatag ggcgcattgt
     4261 cgttgacgtc gatggtattg agctcgctga actgattaac atggtctgcg ataacggcta
     4321 ctcccttcgt gttgttgatg aatctgacca gacctcagca gaatgcacgc caccatttgc
     4381 tacccttacc ggcatacgct gcagtaccgc acatatcacg gaaacggaca acgcctggtt
     4441 gtactcgctg tcacaccaga ccagtgactt cggtgaatca gaatggattc attttacagg
     4501 tagcggatat ctgttacgta ccgatgcgtg gtcatacccg gttctgcggc ttaaacgcct
     4561 ggggctgtca aaaacgttcc gtcgtctggt cgtcacactc atccggcgtt atggcgtcag
     4621 tctcattcat ctggatgcca gcgctgaatg cctgccgggt ttacccactt tcgactggta
     4681 accaggaaca acatgaaatc attaaccacg gaaacagcgc tggatattct gattgcgtgg
     4741 ctgcaggaca atatcgactg cggatcggga attatctttg acaacgatga ggataaaacg
     4801 gattcagcag cactgttgcc ctgtatcgaa caggcccggg aggatgtccg taccctgcgt
     4861 catctgcagc ttctgcacca gaaccggtga agctcactca ccagacttca ttcctctgac
     4921 gccagcctgc acagggctgg cgttttcatt tatctgcaaa aaggaatatc gattatgtca
     4981 gatatcacaa tctcccgtcc ggaagtggtc aacgggcata cggacgttat ctgctcaacc
     5041 agcattcgcc acattctggc tgtacgaaag agtacactgt tgcaaatcga cacacttatc
     5101 cgacaactgg ctgaaatctc ggtgctgacg gaaagcatcg gaggtaaaac tgccccggac
     5161 tgggccatga aacaggattt tcgctgcggc tgctggctga tggagaaacc cgaaacagca
     5221 atgaaagcca ttacccggaa tcttgatcgc gaaatctggc gtgacctgat gcaacgttcc
     5281 gggatgctgt cattaatgga tacgcaggcc cgtgatacat ggtaccggtc actggagtac
     5341 gataattttc cggaaatcag cgaagcgaac attctgagca catttgaaca actgcaccag
     5401 aataaggatg aggtgtttga gcgaggggtg atcaacgtct tcagagggct gaactggaat
     5461 tacaaaacca attgcccctg taaatttggc agtaaaatta tcgtcaacaa tctggtgaga
     5521 tgggaccggt ggggataatg atatggtcac ccccacctta acagcagtac gctgttaagg
     5581 taaatccctt taacggagag accatcatgc agaacatcac acttattggt atcgatctcg
     5641 gaaagcactc cttccacatt cattgtcaag acaagtctgg taaggctctt ctgcgtaaaa
     5701 aatttacccg tactaaactg atgcagtttc tggcgacatg tccgccctca gttgttgtaa
     5761 tggaagcctg tgcgggtgca cactttatgg ctcgctgtat ctctgatttt gggcatgaaa
     5821 ctaagctgat ttctccacag ttcgttcgcc cgtttgtcaa aagtaataag aatgattttg
     5881 ttgatgcaga agccatatgc gaagcggctt cacgaccatc aatgcgtttt gtccagccaa
     5941 gaactgagac ccagcaggcc atgagggcgt tacaccgggt cagagaatcg ctcatcagag
     6001 ataaggtgaa aacaaccaac cagatccacg gtttcttgct ggaatttgga atcagcctgc
     6061 cgataggtga tgctgtaata aaacggttgt ctctcgtgtt ggctgaacac gaaatccccg
     6121 aatatctgag ccgccttttg gtgaggttgc atacccacta c
//
LOCUS       DAZPAB010000207         6103 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZPAB010000207 DAZPAB010000000
VERSION     DAZPAB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6103
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.207"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..84
                     /locus_tag="ACHUPG_003542"
     CDS             <1..84
                     /locus_tag="ACHUPG_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863000.1"
                     /translation="YESVCIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(88..1478)
                     /gene="tcyP"
                     /locus_tag="ACHUPG_003543"
                     /pseudo
     CDS             complement(88..1478)
                     /gene="tcyP"
                     /locus_tag="ACHUPG_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(1611..2201)
                     /locus_tag="ACHUPG_003544"
     CDS             complement(1611..2201)
                     /locus_tag="ACHUPG_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0863001.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2364..3032)
                     /gene="hxpB"
                     /locus_tag="ACHUPG_003545"
     CDS             complement(2364..3032)
                     /gene="hxpB"
                     /locus_tag="ACHUPG_003545"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR0863002.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3179..3715
                     /locus_tag="ACHUPG_003546"
     CDS             3179..3715
                     /locus_tag="ACHUPG_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR0863003.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(3756..4616)
                     /locus_tag="ACHUPG_003547"
     CDS             complement(3756..4616)
                     /locus_tag="ACHUPG_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR0863004.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4722..5012)
                     /gene="ghoS"
                     /locus_tag="ACHUPG_003548"
     CDS             complement(4722..5012)
                     /gene="ghoS"
                     /locus_tag="ACHUPG_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR0863005.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5113..6042)
                     /gene="pfkB"
                     /locus_tag="ACHUPG_003549"
     CDS             complement(5113..6042)
                     /gene="pfkB"
                     /locus_tag="ACHUPG_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR0863006.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1566 a         1543 c         1512 g         1482 t
ORIGIN      
        1 tatgagtcag tatgcatcgc cgcgatggaa catttaaaaa gtaaagcaac ggcccctata
       61 caaaacggac cgttgccagc ataagaatta acggtgtgcc agttcggcgt cgtcttcgct
      121 atccagaatg gctttatcgg tttgcttcag ccactggctg gtcagcgtgc cagctgtcat
      181 cgagccacta acgtttagcg ccgtacggcc catgtcgata agcggttcaa cggagattaa
      241 cagcgccacc agggttactg gcaggcccat cgcaggcagc acaatcagtg cggcgaaagt
      301 tgcaccacca ccgacaccgg caacgcctgc ggaactaacg gtaacaatac cgaccagcgt
      361 cgcaatccac atcgggtcca gcgggttaat gccaaccgta ggcgcaacca tcaccgcagc
      421 attgccggat acaaaccggc gcagccgttc tgaccaatgg ttgcaccgaa agaggcggcg
      481 aaactggcga tggattcagg aacgcccaga cgacgcgttt gtgcttccac attcagtggg
      541 atagacgcag cactggaacg gctggtaaag gcaaacgtca gcacaggcca taccttacgg
      601 aagtacttca gcggactcac gccattaatg cccagcagaa tgccatgcac agcaaacata
      661 atcagcagac cgaggtagga cgcgacaacg aaacttccca gtttgatgat gtcttgcaag
      721 ttagaacctg caaccacttt ggtcattaga gccagaacgc cgtaaggggt caactgcatg
      781 acaaggcgaa ccagtttcat cacccagctt tgtagggtat cgatagcggt taagacgcgt
      841 tcccctttcg gcgcatcatc cttcagcagt ttcaatgcag ctacgccgag gaatgcggca
      901 aaaattacca cgctgataat tgacgtcgga ttggctccgg taagatcggc aaacgggttt
      961 ttcgggataa aggacaagac cagctgcgga acgctctgat cagagacttt accaacatag
     1021 ttactttcaa tggcgttcag acgtgcagtt tctgcaccac cctgaaccag accttcagcc
     1081 gtcaaaccaa acaggttggt gaccagcaca ccgaccagcg ccgcaatcag cgtggtaaac
     1141 aaaagcgtac cgatggtcag aaaactgatt ttgcctaact gagatgcgtt atgcagacgg
     1201 gcaaccgcgc tcagaataga ggcgaagact aacggcataa cgatcatttg cagcagttga
     1261 acatagccgt taccaacgat gttaaaccac tgtacagaat ctttaagtac ctggctgtca
     1321 gaaccataaa tggtatgcag ggcaaggcca aaaaccacac ccatcaccag acccaccagc
     1381 acttttttcg ccagactcca ctgtttatgg cgggtctgag ccagcgcaaa cagcagtaca
     1441 acgaacacca cgatgttcgc aattaatgga aagttcatcc ccgttctcct gatcttatta
     1501 tttctatcac tgcgtagcag tcatagtgtt ggcgcaagat taacagaact gtgagctggc
     1561 tcttatattc aaatggaatt gcttattcca gaaaggtgat ttttgccttt ttagtattca
     1621 acctgatgct taataagccg atgaaactgg atctgcaagg tattgatcat ttgcgatgac
     1681 caacgaacag cgctgttctc gggtaatgaa tggggcatcc ataccgacca gacaaataat
     1741 gccatgcaga taaaacgttc cagttggttg cccttttggg gaaccaggat aggcaagcgg
     1801 aaacgccagc ggcatggcca gagcagggga acaccggcgg gtgtcagcat atcggcaagt
     1861 atgtggctca aataacccag caccattcct tgtagcgcat cagccggaat gaaccagcct
     1921 tccggaacct taaggtaaaa ggttgccagc agcgcaaata ccgccagcag actgtgggta
     1981 aaaccacgat gcccaaaagc gcgggcgatc ggttttgata tccattttaa tcgctgccca
     2041 agaaacgact ttgggtgatc gatgtccggt aacaaacacg tcaggattgc ggaagggaca
     2101 atatgccacc agtcaccctg tgccagcacg ggcgtcagct cggcattttt ggcaaatacc
     2161 gcacaagcaa tagaaaagag aaggtgacct tccgccgtca tgataaaact cactaaactg
     2221 tcgatacgta cagtatagga tttttataca gtgcacggaa gtggtgacgg tgtaactctt
     2281 tctcttaaat tgacgattaa tccgtcagtt gaatgcaaat tcagataaag aaccaggggc
     2341 agcgtgatca ctgcccctgg tcattaaccg agaaggtctt ttgcggtgag ttctgtcagc
     2401 gatgagagtt tgacgtttgc taatacaaaa cgtggatcat tttgcgcttc tggcgcagga
     2461 acgacgatgg aacgcatgcg ggctgcttta gaagcgatca tgccatttac cgagtcttcc
     2521 agcgccacgc aggtcagtgg gtcaacgccc agttttgctg cgcagtcgag atatacttgc
     2581 ggatgcggct tgctgtaagg cagtttttca gccgaggcga gggcatcgaa actgtcgcgt
     2641 aagtcaaaca tggtcaacac tttttccagc atatgtagtg gtgacgcgga ggccagtccc
     2701 accaataaac cttgttcttt gcataacgca acagcttcgc gcacgcctgg taataatgga
     2761 cgtgtctctt caaccagtga aatggcacgg gcaataaccc gttctactac ttcctgacgg
     2821 cttggcccat tccacggttg ccgggcgtac caaagatcga ccaccatatc gatgcgtaaa
     2881 cctaaggtgt ccggcagctc gttacgacgg gagatatcta cccccaggct tgccatcaca
     2941 tccagttcgg ctcgatccca taaaggttct gagtcgataa gtaatccatc catatcaaaa
     3001 attgcagcaa gaatctgacg cggggttgac attacaacct ctcctttaga gcattatgtg
     3061 taaggtatta agccgtgctt atgattaagc gcctatcata tcgcctttaa agaatgggcg
     3121 aaaacagata tctacaggta aactaagtga aacgacgcgt cttaatcaag gagaactcat
     3181 gacgtatcaa caagctggac gcattgctgt tttgaaaagg attttgggtt gggtgatttt
     3241 tatacctgca ttgatctcta cattgatttc tttgctgaaa tttatgaata cccggcagga
     3301 aaaccaggaa ggcattaatg cggtcatgct cgactttgct catgtcatga tcgatatgat
     3361 gcaggcgaat acgccttttt tgaatctgtt ctggtataac tccccgaccc ctaattttaa
     3421 tggtggtgtg aacgttatgt tctgggtgat tttcatcctg atttttgtcg gactggcgtt
     3481 gcaggattcc ggtgcccgga tgagtcgcca ggcgcgcttc ttgcgggaag gtgttgaaga
     3541 tcaactcatt ctggaaaaag ccaaagggga agaggggcta acgcgtgaac aaattgagtc
     3601 ccgtattgtt gttccgcacc atactatttt cctgcagttt ttctcactat atatcctgcc
     3661 ggttatttgt attgctgcag gttatgtgtt cttttctctg cttgggttta tttaatcaaa
     3721 aggccgcacg gagcggccat tatcctcaac ccatatcatg ctgctaataa tctatccaat
     3781 gactgctgag caataaccaa atgctgaccg ccaaataatc ttgcacgatt taacagcgtg
     3841 tagagttggt aaaccggttg acgttcaagg aaatcggcag gtagcgggga tactgactga
     3901 tagccgtcat agatttgtgg cggctgttca gtatgcagcg gtaacatcgc taggtcgcac
     3961 tctcggtcac cccagtcgca ggcagggtcg aaaatgtacg ggccatccgg acccagcgca
     4021 cagttgccgg accataaatc gccgtgcaac agagaaggct gcggttgatg cgaggccaga
     4081 cgctgctgaa tatgctcgac gatggcgtcg atattgccga aagcgatccc tttctctgct
     4141 gccagttcca actgccagcc aatccgttgt tcagcaaaaa acgttgacca gcgacgttgc
     4201 caggtgttgg gctgtggagt tgtggagagc gagttatcaa aatcgaggcc aaattgtggt
     4261 tggtcactcc attgatgtaa acgcgcaatt tgctgaccaa gaataaatgc gctatgcgca
     4321 tccagcggac gaggtgggag ataatccatc accagaaaac tgtagtcacg gtcagcgcct
     4381 actgcccaaa ccttaggcac ggtgacggtt ttactacgcg acagtaactc cagttggtcg
     4441 gcttcggcgg taaaaccggg aagcagttcc ctttcatcac atttgacgaa aaagtcatgt
     4501 cctgcatagc gcaaatgcca tgcggcatgg acttctccgc caggcagttc attacgcagt
     4561 tcgatttcgc cttcacctaa ctgctcgctc aaaagacgac tgattgcctg ccacatgatg
     4621 tctctcccat gttgtctgcc agatcataag gttagcgcaa gaatgcggtg taaaaacacg
     4681 agctaacgca caccacagac tgattattgc ccaaaaacca tttattttct gttgctattc
     4741 cattcctccc aggtcgtgat ggtgatttca ggatctttgc cggttgcact ttgggtaagc
     4801 cccgaaacca gttctctaat ttcttcttca ctttgggtac tgataagtcc aaaagtgtta
     4861 gtccccagct catggatatt tccctcatca tccgtcatgg tgagcaaaaa accgtcgcga
     4921 gtcaggtaat tattcaactc gttaatttca gtcaatgtat cctcatgcaa catgacggtt
     4981 atgacataat ggacaagatc gccactggcc atagttcacc cctttgttat cacatgactt
     5041 ttgagcatag tcggagaaac gcgttgccga caggttggtg gtgattcccc caatgctggg
     5101 ggaatgtttt tgttagcggg aaaggtaagc gtaaattttt tgcgtatcgt catgggagca
     5161 cagacgtgtt ccctgattga gtgtggctgc actccccgca gctacgccaa aacggaccat
     5221 ctcttcaaga gaggcatttt ctgccagttt cagtgtcatc gcgccgacca tgctgtcacc
     5281 agcgccaacg gtactctggc ttttcaccgg tggtggcacc acctgaatgc agttttcact
     5341 atcgacaccc agcgctcctt gtggaccaag ggaaacgaca acccgtttgg ccttgccgct
     5401 attaacgatt tcctgcgcgg ctttgcggac atcgtctggc tgggtgagtt cgcgattcac
     5461 cagcgcactg agttcttttt ggttaggctt aaccaactcg atgttaccaa tcgccagtgc
     5521 tgcacttaac gcttcgccag aactgtcgat gatgcagcgg atcccttggt tttgcgcagc
     5581 ggaaatcagt tgggttaatt tttccagctt cacacctggc ggcaggcttc cgcttatgac
     5641 caggatggcc ccggattcaa tttccagaac ttgctcttca agctggcgaa actcatcttc
     5701 atttaatgcc gcgcctggca taacaaaacg atactgttca ccgcttgctt ccacatgtac
     5761 gtgtaaattc tgccgggtcc agtctttggc ttctacagta gcgacgggga cattttcatc
     5821 cgccaacagt gaaaccaggt gttcgccggt cgcgccaccc gccgggaaga tcgctgtggc
     5881 actgcctcca agatgggcaa tggcgcgggc gacgttgatg ccgccgcccc cgggttcgaa
     5941 caccggtgcg gtacagcgca gttttccttc gggataaatt tgcggggtaa ttgttgcgct
     6001 atcgagagag ggcgcaagtg tcaacgtata gatacgtacc atcatttcct cctataggct
     6061 gatttcagtc tggcaccgaa tcagcggaaa gtgaagaaat taa
//
LOCUS       DAZPAB010000208         6076 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZPAB010000208 DAZPAB010000000
VERSION     DAZPAB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.208"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..143)
                     /locus_tag="ACHUPG_003550"
     CDS             complement(<1..143)
                     /locus_tag="ACHUPG_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HFR0863007.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQ"
     gene            379..651
                     /locus_tag="ACHUPG_003551"
     CDS             379..651
                     /locus_tag="ACHUPG_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFR0863008.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(617..1024)
                     /gene="nudG"
                     /locus_tag="ACHUPG_003552"
     CDS             complement(617..1024)
                     /gene="nudG"
                     /locus_tag="ACHUPG_003552"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR0863009.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1111..1749
                     /locus_tag="ACHUPG_003553"
     CDS             1111..1749
                     /locus_tag="ACHUPG_003553"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR0863010.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1758..3065)
                     /gene="ynjE"
                     /locus_tag="ACHUPG_003554"
                     /pseudo
     CDS             complement(1758..3065)
                     /gene="ynjE"
                     /locus_tag="ACHUPG_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3132..3785)
                     /locus_tag="ACHUPG_003555"
     CDS             complement(3132..3785)
                     /locus_tag="ACHUPG_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0863011.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3785..5256)
                     /locus_tag="ACHUPG_003556"
                     /pseudo
     CDS             complement(3785..5256)
                     /locus_tag="ACHUPG_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5229..6076)
                     /locus_tag="ACHUPG_003557"
                     /pseudo
     CDS             complement(5229..6076)
                     /locus_tag="ACHUPG_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1427 a         1623 c         1607 g         1419 t
ORIGIN      
        1 atctggcgat attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga
       61 acggcttgcg cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac
      121 tccagagaat atgtctgatc catagatata aaaaccttat atattaatac gattgtgctt
      181 atgttgttta tgtcatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta
      241 agcaagaaaa aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat
      301 gcgataactc agacgctaaa gttttgccac ccggctaccg ttatcagtag cagagacaat
      361 aacgttaagg agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta
      421 attgcactgg ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg
      481 ttcagttcag gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggatcaa
      541 aattattctg aaggggcggt aattaaagcc gaaggaatac tgctgcaatg ccagcgtgat
      601 gacaaaaccc ttagcactaa tccgctgctc tggcggcgcg taaagccata aacgcctcta
      661 ataatggaat gtcagcaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga
      721 ccagcgcctg atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat
      781 gaagatggat aatccggccc gaaacttctc gctgatggct ggcaacatac tcacccacag
      841 ttgcttcgat gtccaattct tcgtttaact cacgcaccag cgcctgctgc tggctttcat
      901 ccagctcgac tttacctccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc
      961 gttgcgcgag taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt
     1021 tcataaatta cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa
     1081 tcagaaaggc tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt
     1141 aaaccgttgt tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt
     1201 ttaacattag tgggatttgc catcggcgtg ctggctctgc cgtttctggc gctgggctgg
     1261 tatctggcgg cgctagtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg
     1321 ctggcgcggc gcagagggct taccgacgcg ggcggctttc ttgatatttc tctcgatttt
     1381 ctcttttacg cgctggtgcc gtttggcttt attcttgctg caccggagca aaatgcgctg
     1441 gcaggcggct ggttgttgtt tgcgtttatc ggcacgggca gcagttttct cgcctttgct
     1501 gcactggcag caaaacatca gattgataac cccggttatg cacataagtc gttttactat
     1561 ctggggggat taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc
     1621 cctgcatggt ttgcgtggtt tgcgtggttt gcgtggtttg cgtggatctt cggtgcgttg
     1681 tgctggatga cgacctttac gcgggtgtgg agtgggtatc tgacgctgaa gtcgctccag
     1741 cgtcagtaat tgcaatgtta tttgctactg cccgggccgc gttcaccggt tgcaaccgga
     1801 ttttttggat cgctgctcca ttcgtaccag ccgccgtcat acacggaaac attcttccaa
     1861 cccatggcgc gtgcgtacat aaaggtttcg gacgcgcgcc agccggtgcc gcagtagaat
     1921 gaaacttgct gatctggttt gatattccat gctttccaca tagcggtaat atcatcggcg
     1981 ctacgcatgg tgccatccgg gttatagaaa tcttccatat gcgtcgagtc gctaccagcg
     2041 tgtccccaac gtgctccggc tatttcacct tttggtttaa tatagctgta accgctggtc
     2101 gtaccgataa attctggcca cgaacgaatg ctcaccagcg atgcatcctg gcgatgcagc
     2161 agtccacggg cctgttccat atcgagcatc aactgcggtt gtgccgggat cttcacgccg
     2221 aaatccggtt ccgctttcac tttcggtggc gttcctcgct caacaggcag tcccgcgtcg
     2281 gaccaggttt gccagccgcc atccagcaga cgcacatctt tcacgcctgc gtagagcata
     2341 atttgcgcca cacgcgctcc agcgtatacg tcacgcccgt acagaatgac cgtggtgtca
     2401 tggcgaatgc cgtgttttgc cagcatcgct tttagttgtt catcagaaac tttgttccac
     2461 agcggttcgc tttccacttc gttggtatcg atgtagtcag cgccaggaat atggctgata
     2521 aggtaaagct taggagcgcc ctaggccgct tcaatgactt tccagtcacc gacaggtttc
     2581 gccgtaacct ctttaccttg ttgcaggtca tgcagccatt gcggataaac cagctgctca
     2641 aagtgcggca gtttttgtag acgggaaggt tcgcttagcg cgtcactcag aatggagata
     2701 tgcgtaaaac ctgctttttg cagtcgcgtt ttgacggcgt caacatcttt gccattaccg
     2761 tacagcgcca ccggggcatc ggctttcagg ttatgttgct tgatccacgc gttgagctgt
     2821 tcggtgctca ttttgtccag ccagctagca gagaggttta aggcggcagg ttcatgacca
     2881 gaagggccat ttaaggtttg tggccagccg ttataaaacg cgctggggcg agtatctatc
     2941 gctttgccat tttgttgctg aagttggtca agtgtaagag gcttcgccag ttcagcggcc
     3001 cacgaagaag cgcaagccag ccctaaagcc attgccagcg cggtcatttg agaaacacgt
     3061 ttcatcgaaa atcccgacat tgtagttttg agtgcgccat tctgaactga cgcattgaga
     3121 aaaactttgc gttatcgcag tttgttgtaa ttttctgacc actgcgccat atccagaaca
     3181 gaactatcag caggaacatc ctggagatcg tgcgttacct gaacgacggg gatcgccagg
     3241 gcgcgaactt cgctgaacac ccactggcga aaattatcgc gcagagccac atcaagacgg
     3301 ctgaatggct catccaggag taacgctttt ggctgggcga gaagggcgcg tagtagagca
     3361 acgcgcgctc gctgaccgcc agacaaagtg gcaggatcct gatggaaagc tccgtcaagg
     3421 cctgaacgct caagtgcatc attcacggca ttacgtcggg cattcccctt aagtgtcgcc
     3481 ggtagcgcca gcagtaaatt ttgcccgacg ctgaactggt caaataacag tgcatcctga
     3541 aaaagaatgc caatctgacg ctgtgcggtg ggtagcatgt caatccgttg ctcattgagc
     3601 catagctcac ctgtacaaga aaactgtccg gccagtgcac caatcatcca tgaaaacaga
     3661 gtggattttc cacagccaga cggccccatt aacgtgacga tgtcaccttt atcaaccgta
     3721 aagttaacgt ttgtcagcaa gcggctttct ggtaaacgta gcgaaacatt tttcacgcag
     3781 agcattagcg gattccttgt ctgacataac ctacccattt tgcgactaac gcggtcaggg
     3841 caaaaataat aagcggtaac aacagttgcc ataacgcctg ggcggcgaga ataccgttgc
     3901 tgccgccgct gcttaatgcc accgcctcag tggtgagcgt cggaaaacgc cccgcgccca
     3961 gccacagcgt cggcatatac tgcgcaatac tgactgaaaa tcccaccgcg aaggcaatca
     4021 gcacagggcg caacatgagt gggcatttca cgtaaaagaa gattttggcc cgcgaccagc
     4081 ccagtgtttg cgcaatcaaa attaaccgtg aatcaatgcg ctgccaggcg ggttgcagga
     4141 taaacagcat ccacggcatt acccacagca gatgccccca gaccaccgcc gtccagcttc
     4201 catccaggtt cagccatagc gccagcgtgt actggcccgc caccagtggc agagcgggca
     4261 atagaatggg caaccatagc cacaactggc gacgctgtgc cccccattcc agccacagca
     4321 gtaacaggag caaagcgatg aatgttgcta ccagccccat tgtcagactg ttgatgagtg
     4381 cttcactatt gatcgtcgac tgatccgcga ggatcgccag cagaaccaca cacagcacac
     4441 cggttaaggg taaaaaactc gccagcgtag tgcctggtaa taaaggtgtg gcaggcttgc
     4501 gaatgccatc tacgcgggga atagtgcgcc gccagctgcg ccaaaacgta ggcggcgagt
     4561 aacagcatca gcagcagact ggcgagtgcg cctttcgttt gttgatccgc gtcgccctga
     4621 gttaaccact gccagctaat tactgccagc gtcggtggat tacctggccc aagaataatc
     4681 gccacatcca cgaccgaaag cgaccaggca acaatcgcca gcatcgccat tgccagcggc
     4741 tgtagcccag cgaatccagc acaatgacct gctgcaacag ccgtttttcg ctcaatactg
     4801 ccactaagat ccacagcaga aatgcgcttt ctttcactgc aagggtgagg cccagaccga
     4861 tccaaatcgg tccattggcg gagtgaaata cgggaaatag tcatatagca gccctccgtc
     4921 agcaaagagc agcagggcgc tggtggcaaa agccacatgg ggaatggcga gcagccacgg
     4981 cagacgggcg cacatacgct gccatttcgg cccaggccat agcgccacaa tcaccagcag
     5041 ggcaatcaac aatgccccga ccgccgcgat ggttgtcgac accagcgttg ccagtaatgc
     5101 ctgcggtaac tgtggatcgg caaataacgc ctgccagcgt gtcaacgaca acgcaggcga
     5161 tatcagcgtg agagctgccg ggatcagcgg tgcataaatc accgccacca tcgcccacag
     5221 caaaaagatt aatgcgtacc gtaacggcgt aaccattctt gttccagtgc attcacccat
     5281 cctgcgtgcg gttcagccag taccggcggc agatcctgcg gcattcttga ttgcaatatt
     5341 tcgcgctgcc cgtcaggcag tttttgcgga tcgagaacag aaggatcgcc ccagacaacg
     5401 ggatctgctt tacgcagttg cgcatcgggt gagagcagga aattggcaac taccttcgcc
     5461 gcagcactgg cattcgcgtt ggctggaatg gtgacgaaat gcacgttgcc aatcatcccc
     5521 tcgcgaaagc caaaactgta actgcttgcc ggcaaatcgc cgctggcgat tttttgctgc
     5581 gcatgtgcgg ggttaaaggt cagcgacagg cgcaatgtgc cggctttcag cagagcatcc
     5641 atccgcgcgg gcgaaggcgg gaaatccttt ccttcgcgcc acaaatacgg atgcagtgca
     5701 tcaagatatt gctacaaggg cgcagtgaca cgggcgaaag tcgcatcgtc cggcgcttct
     5761 tttaatgcgg cgggatctgg cgtcagcata atcagcaact gttcaagaaa cgccgtgccg
     5821 gtaaagtccg gtgggcgcgg ataagtaacc gtgccgggat tggctttagc aaactccagt
     5881 aaggcttgcg gcgtttgtgg tggctgtggc gtaacatcgc ggcgggcgat aaacgtcagt
     5941 tgtgcgccgc cccagggcga ttccgcacct tgtgtcggca ctgaaaaatc tccccgcacc
     6001 ggcagctgtg tgtcgacata gcgccagttg ggcagagtct ccgcccagcc cgtttgtaat
     6061 aaattggcct ctttta
//
LOCUS       DAZPAB010000209         6064 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZPAB010000209 DAZPAB010000000
VERSION     DAZPAB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.209"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            165..1286
                     /gene="ompC"
                     /locus_tag="ACHUPG_003558"
     CDS             165..1286
                     /gene="ompC"
                     /locus_tag="ACHUPG_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR0863012.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1398..2453
                     /gene="apbE"
                     /locus_tag="ACHUPG_003559"
     CDS             1398..2453
                     /gene="apbE"
                     /locus_tag="ACHUPG_003559"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR0863013.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2527..3591
                     /gene="ada"
                     /locus_tag="ACHUPG_003560"
     CDS             2527..3591
                     /gene="ada"
                     /locus_tag="ACHUPG_003560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR0863014.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3591..4241
                     /gene="alkB"
                     /locus_tag="ACHUPG_003561"
     CDS             3591..4241
                     /gene="alkB"
                     /locus_tag="ACHUPG_003561"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR0863015.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4317..5960
                     /gene="yojI"
                     /locus_tag="ACHUPG_003562"
     CDS             4317..5960
                     /gene="yojI"
                     /locus_tag="ACHUPG_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR0863016.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1443 a         1520 c         1645 g         1456 t
ORIGIN      
        1 atctatagcg ataaatgaaa catcttaaaa gttttagtat catattcgtg ttggattatt
       61 ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc agtatgcagt
      121 ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa gttaaagtac
      181 tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct gaagtttaca
      241 acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac tatttctctg
      301 acgacaagtc tgtagatggc gaccagacct acatgcgtct tggcttcaaa ggtgaaactc
      361 aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag ggcaacagcg
      421 ctgaaaacga aaacaactcc tggacccgtg tggcattcgc aggtctgaaa ttccaggatg
      481 taggttcttt cgactacggt cgtaactacg gcgtagttta cgacgtaact tcctggaccg
      541 acgtactgcc agaattcggt ggtgacactt acggttctga caacttcatg cagcagcgtg
      601 gtaacggctt cgcaacctac cgtagcaccg acttctttgg tctggttgac ggtctgaact
      661 tcgctgttca gtatcagggc aaaaacggta gcccggaagg tgaaggcatg accaacaacg
      721 gtcgtgaagc tctgcgtcag aacggtgacg gcgttggcgg ttctattact tatgattacg
      781 aaggcttcgg tatcggtgct gcagtttcca gctccaaacg tactgatgac cagaacttcg
      841 gtctgaaccg ttatgatgag cgttacatcg gtaatggcga ccgcgctgaa acctacaccg
      901 gtggtctgaa atacgacgcg aacaacatct acctagctgc tcagtacacc cagacctaca
      961 acgcaactcg cgtaggcaac ttgggttggg cgaacaaagc acagaacttc gaagctgttg
     1021 ctcagtacca gttcgacttc ggtctgcgtc cgtccctggc ttacctgcag tctaaaggta
     1081 aaaacctggg tgtcatcaat ggtcgtaact acgacgacga agatatcctg aaatatgttg
     1141 atgttggtgc gacctactac ttcaacaaaa acatgtccac ctacgttgac tacaaaatca
     1201 acctgctgga cgacaaccag ttcactcgtg acgctggcat caacactgat aacatcgtag
     1261 ctctgggtct ggtttaccag ttctaatctc gatggatatc gaacaaaggg cctgcgggcc
     1321 cttttttcat tgttttcagc gtacaaacac agttttttgg tgtactcttg cgaccgttcg
     1381 catgaggata atcacgtatg gaaataagct ttacccgcgt ggcactgctg gttgccgcgc
     1441 tcttctttgt tggttgcgat caaaaaccac aacccgccaa aacccacgct actgaagtta
     1501 ccgttcttga aggcaaaacc atgggtacct tctggcgtgc cagcatcccg ggcattgatg
     1561 ccaaacgcag tgccgaactt aaagaaaaga ttcagaccca gctggacgcc gacgatcagc
     1621 tgctttcgac ctataaaaaa gattccgcgc tgatgcgctt taacgactcg caaagtttgt
     1681 cgccgtggcc ggtaagtgaa gcgatggccg atatcgtcac cacctcgctg cgcattgggg
     1741 caaggaccga tggtgcgatg gatattaccg tcgggccgct ggtgaatctg tggggctttg
     1801 gcccggaaca acagccggtt caaattccga gccaggaaca gatcgatgcg atgaaagcca
     1861 aaaccggctt acagcacctg acggtcatca atcagtcgca tcagcaatat ctgcaaaaag
     1921 acctgccgga tttatatgtc gatctctcca ccgtcggtga aggttatgcg gcggatcacc
     1981 tggcacgctt gatggagcag gaagggattt cccgctatct ggtgtcggtg ggcggcgcgc
     2041 tgaacagccg tggtatgaac ggtgaaggcc agccgtggcg ggtagcgatt caaaaaccaa
     2101 ccgataaaga aaacgcggtt caggctgtgg tggatatcaa cggtcatggg attagcacct
     2161 ctggcagcta ccgtaactat tacgaactgg acggcaaacg tctttcccat gttatcgatc
     2221 cgcaaaccgg gcgtcccatc gaacacaatc tggtatccgt gacggtgatt gctccgacgg
     2281 cgctggaagc cgatgcctgg gatactggct tgatggtact cgggccggag aaagccaaag
     2341 aggttgttcg ccgggaaggg ctggcggttt atatgatcac caaagaaggc gatagcttta
     2401 aaacctggat gtcaccacag tttaaaagct tccttgtcag cgaaaaaaat taaagcgcaa
     2461 gattgttggt ttttgcgtga tggtgaccgg gcagactaaa ggctatcctt aaccagggag
     2521 ctgattatga aaaacgccac atgcttaact gacgatcaac gctggcaatc tgtcttagcc
     2581 cgcgacccga atgccgacgg cgaattcgtt ttcgccgtgc gcaccacggg tatcttttgc
     2641 cgtccttctt gccgcgccag acatgccttg cgggaaaatg tctccttcta cgcaaatgcc
     2701 agcgaggcac tcgccgccgg atttcgcccc tgcaaacgtt gtcagccaga caaagccaat
     2761 ccccggcaac atcggttgga taaaatcacc cacgcgtgtc gactgctgga acaggaaacg
     2821 cctgtaacgc tggaagcctt agccgaccag gtggcgatga gtccatttca tctacatcgg
     2881 ttgtttaaag ctactaccgg aatgacgcct aaagcctggc aacaggcctg gcgcgctcgc
     2941 cgtttgcgcg aatcgctggc gaaaggggag agcgtgacga cgtctattct taacgccgga
     3001 ttccccgaca gcagcagtta ctaccgcaaa gccgatgaaa cgctgggcat gacggctaaa
     3061 caattccgtc acggtggcga aaatctggcg gtgcgttacg cgctggctga ttgtgagctg
     3121 ggtcgttgcc tggtggcaga aagcgagcgg gggatttgcg cgatattgct gggcgatgat
     3181 gacgcgacat taatcagcga gttgcagcag atgtttcccg ctgccgacaa cgcgcctgcc
     3241 gatctgacgt ttcagcaaca tgtgcgtgaa gtgatcgcca gcctcaatca acgcgatacg
     3301 ccgctgacgt taccgctgga tattcgcggc actgcttttc agcaacaagt ctggcaggca
     3361 ctgcgcacga taccttgtgg tgaaaccgtc agttatcagc aactggctaa cgccatcggc
     3421 aaaccgaaag cggtacgggc cgttgccagc gcctgtgccg ccaacaagct ggctatcgta
     3481 ataccctgtc atcgggtggt ccgtggtgat ggcacacttt ccggttaccg ctggggcgtg
     3541 tcgcgtaaag cgcaactgct gcgccgcgaa gctgaaaatg aggagaggta atgttggatc
     3601 tgtttgccga tgctgaaccg tggcaagagc cactggcggc tggtgcggta attttacggc
     3661 gttttgcttt taatgctgcg gagcaactaa tccgcgatat taatgacgtt gccagccagt
     3721 cgccgtttcg ccagatggtc gctccaggcg gatataccat gtcggtggcg atgaccaact
     3781 gtgggcatct gggctggacg acccatcggc aaggttatct ctattcgccc attgatccgc
     3841 aaacaaataa accgtggccc gctatgccac aaagctttca tgatttatgt caacgtgcgg
     3901 ctacggcggc gggctatcca gatttccagc cagatgcttg tcttatcaac cgctacgctc
     3961 ctggcgcgaa actgtcgctg catcaggata aagacgaacc ggatctgcgc gcgccaattg
     4021 tttctgtttc tctgggctta cccgcgattt ttcaatttgg tggcctgaaa cgtaatgatc
     4081 cgctcaaacg tttgttgttg gaacatggcg atgtggtggt atggggcggt gaatcgcggc
     4141 tgttttatca cggtattcaa ccgttgaaag cggggtttca tccactcacc accgactgcc
     4201 gctacaacct gacattccgt caggcaggta aaaaagaata aaaataagaa ttattattgc
     4261 tgtgcgcgcg aagatgttta aactgcgggc tgtaattcat tgtccgggtt ttctgcatgg
     4321 aacttcttgt acttgtctgg cggcagtatc gctggccatt tatcagtgtg atggcgctaa
     4381 gcctcgccag tgcggcatta ggcattggct taattgcttt tatcaatcag cgccttatcg
     4441 aaacggcgga taccagtctg ctggtgttac cggagtttct gggattattg ctgctgttga
     4501 tggcagtcac tctcggatcg caactggcgc tcaccacttt ggggcatcac ttcgtttacc
     4561 gactgcgtag tgaatttatc aagcggattc tggataccca cgtcgagcgc attgaacaac
     4621 tcggtagcgc ctcgttgctg gcgggattaa ccagcgatgt gcgcaatatc accattgctt
     4681 ttgtgcgtct gccggaactg gggcagggga tcattctcac tatcggttca tcggcgtatc
     4741 tgtggatgct gtcgggcaaa atgttgctgg taacggctat ctggatggcg atcaccatct
     4801 ggggcggttt tgtgctggtg gcgcgggtgt acaaacatat ggcgacccta cgtgaaaccg
     4861 aagacaagct gtacacggat tttcaaactg tacttgaagg gcgcaaagag ctgactctga
     4921 accgggaacg cgccgagtat gtgtttaaca acctctacat ccccgatgcg caagagtatc
     4981 gccatcatat tattcgcgca gacaccttcc atcttagtgc cgtgaactgg tcaaacatca
     5041 tgatgctggg cgctatcggc ctggtgttct ggatggcgaa cagtctcggt tgggctgata
     5101 ccaacgttgc tgcgacctat tcgttgacgc tgttattcct gcgtacgccg ctgctttcgg
     5161 cggttggcgc attgccgacg ctgctgacgg cgcaggtggc gtttaacaag ctgaacaaat
     5221 tcgcgctcgc gcctttcaaa gctgagtttc cgcgcccgca ggcgtttccc aactggcaaa
     5281 cgctggagct gcgtaacgtg acgtttgctt atcaggataa cgcgttttcc gttggtccga
     5341 ttaatctcac catcaaacgt ggcgagctgc tgtttctgat tggcggtaac ggtagcggaa
     5401 aatcgacgct ggcgatgttg ttgacgggct tgtatcagcc acaaagcggc gaaatcttgc
     5461 tggatggcaa acctgtcagc ggcgaacaac cggaagatta tcgcaaactg ttttcggcag
     5521 tgtttaccga tgtctggctg tttgatcaac tgctggggcc ggaggggcaa cccgctaacc
     5581 cgcaactggt tgagaagtgg ctggcgcagc tgaaaatggc tcataagctt gagttaagca
     5641 acgggcgtat tgttaacctg aagttatcaa aagggcagaa aaaacgcgtg gcgctgttgc
     5701 tggcgctggc agaagaacgc gatattatcc tgctggatga atgggcggtg gatcaggatc
     5761 cacacttccg tcgtgagttt tatcaggtgt tgctgccgct gatgcaggag atgggtaaaa
     5821 ctattttcgc tatcagtcat gatgatcatt actttatcca cgccgaccgc ctgctggaaa
     5881 tgcgcaatgg gcaacttagc gagctgacgg gcgaagagcg cgatgccgct tcgcgtgatg
     5941 ccgttgcccg gacggcataa tattacgacg ctgacctggc ggactgctgc cgtcaggtca
     6001 atatgctaaa aatcccttca tttctaataa ccctataatt aatcaacgaa attataatgt
     6061 ttct
//
LOCUS       DAZPAB010000210         6032 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZPAB010000210 DAZPAB010000000
VERSION     DAZPAB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.210"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..464)
                     /locus_tag="ACHUPG_003563"
     CDS             complement(<1..464)
                     /locus_tag="ACHUPG_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFR0863017.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDP"
     gene            complement(722..1357)
                     /gene="msrQ"
                     /locus_tag="ACHUPG_003564"
     CDS             complement(722..1357)
                     /gene="msrQ"
                     /locus_tag="ACHUPG_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR0863018.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            complement(1358..2362)
                     /gene="msrP"
                     /locus_tag="ACHUPG_003565"
                     /pseudo
     CDS             complement(1358..2362)
                     /gene="msrP"
                     /locus_tag="ACHUPG_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            complement(2471..2884)
                     /gene="hiuH"
                     /locus_tag="ACHUPG_003566"
     CDS             complement(2471..2884)
                     /gene="hiuH"
                     /locus_tag="ACHUPG_003566"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR0863019.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3017..3688
                     /gene="hprR"
                     /locus_tag="ACHUPG_003567"
     CDS             3017..3688
                     /gene="hprR"
                     /locus_tag="ACHUPG_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR0863020.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3688..5046
                     /gene="hprS"
                     /locus_tag="ACHUPG_003568"
     CDS             3688..5046
                     /gene="hprS"
                     /locus_tag="ACHUPG_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR0863021.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(5154..6005)
                     /gene="hchA"
                     /locus_tag="ACHUPG_003569"
     CDS             complement(5154..6005)
                     /gene="hchA"
                     /locus_tag="ACHUPG_003569"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR0863022.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
BASE COUNT         1687 a         1283 c         1402 g         1660 t
ORIGIN      
        1 tcaggatctt tacattcgaa taagtaacga acgcctttct tgcctgattt ataggtgagt
       61 attttgtatc catcgtaatc atatttacag gatgttgttt cattatttct atggaattcg
      121 acaatgccgt cctcaatgcc aatcatttcg atatctgtcg gataaccttt gcgataataa
      181 tctttaattt cagcaaatgt tttggtttta tctgcatccg ctttcttctg aaagacggga
      241 tcaagtttgc cactttgcag taaagggtaa acggattgcc agactccatc ccagtcactg
      301 agcgttcggt tttgtacatt ggtatcatca aaaacaccat tagcagcttt ttgttcgacc
      361 tctgttaagg gtttgccatg agaatgatga ccatgcgaaa aggcaggagc gctaacaata
      421 aagacaccta aagcaacagc cagtttatga agacgaatcg ccaaaacaat ttcctccaga
      481 gttaatgtac attatgtgta atgttatatt gtaacatatg aaaaaatgca acaactcatt
      541 aggaaatgag agcatggtat tctcaggtgt attgaactat cttattgaaa taaaataaat
      601 tattctggtg atgaaaggtt gatgacaaac gaaacataac ttgatgcgat ctgtgtgggc
      661 cggatagggc gctcgcgccg catccggccg ggataacgcg aagtttgtcg gcaatatgaa
      721 gttatagaga taatttgttg tgggcctgct tgcgtaaccg gttgaaaagg gaaagcaact
      781 tcttataccg taaggctaaa agcagtacag ccagcccggc gtagatgagt ggctgcggtg
      841 aaataatctt cactgaccac agataatgta ttggggccag gatcgcgaca agatagacga
      901 agttatgcaa ctgttgccag tgtttgccga gttttcgctg catcgactgg gttgaagtga
      961 acgctaaagc aagcaaaata acccagctga taattcctaa cgttaaataa ggccgggtaa
     1021 ttaactcctt acctaataac gccagattat tcacgcccaa ctccagcaat gcgtaactgg
     1081 ttaaatgcag tgtcgcccag gcaaagcacc ataagcctaa caggcggcga gtgcgtatca
     1141 ataacggctg ttttgcgtag cgtgccagag gtgtgattaa caaggccgca agcaaaaatt
     1201 tcagtgcagt gcgaccggta aaatgctgaa tatctttcac cggatcggca cccagtccac
     1261 cgtgattgat cgcccagacc agccagagaa acggcaacaa tccggcaaga tgcaggcaaa
     1321 ctttcagcca tatcacttgt tttgctgtca gacgcattta gaaattctcc cgcaaatcca
     1381 ggccacgata cagcgatgcc acctggtcgg cgtaaccatt aaacagtagt gttggctggc
     1441 gctgaacatc gagaatgccg cctgaaccaa taaatcgttc ggtagcctgt gaccagcgcg
     1501 ggtgatcaac atgcggatta acgttggcgt aaaaaccgta ttcgtcaggc gctgccagat
     1561 tccaggtggt tggcggacgc tcgcgggtca gcttaatact gacgatcgat ttaatccctt
     1621 taaagccata tttccacggc acaatcagtc gcaccggcgc gccattttgc ggtggtaacg
     1681 ccttgccata aacacctacg gtcatcagtg tgagcggatg cattgcttcg tcgagacgca
     1741 atccttcgac ataaggatat ttcagcccgc cgccgataaa gcggtcctgc tggccaggca
     1801 tctgttccgg cgcataaatc gtttcgaaag cgacatactt cgcattgctg gtgggttcgg
     1861 caagcgccag caatttgtgt agcggaaaac caatccacgg caccaccatc gaccacgctt
     1921 ccacgcagcg catacgataa atacgctctt ccagcgggaa gcgacgggtt aaatcatcat
     1981 gatcgagggt caatggtttt gccacttcgc cgctgatttt cagtgtccat ggatcggttt
     2041 tcaggctacc agcattagcc gcgggatcgt ctttatccag cccgaattca tagaagttgt
     2101 tataaccgga gactttatct gctggcgtca gtggcaggtt attttgctag gcggcaggct
     2161 tgctgaactc cagcggtttt ccggcggggg cgggcggacg atcgttccct ttaaaccagc
     2221 taagcagatc ggcatgcgca gcgtgaggca aagaaaatgc agctgcgctg atgcccagtg
     2281 cttttaacac ctgccgacgc ttcataaaga ataccgactc ggccgtaaca tctgattctt
     2341 ttaaaaattg cttctttttc ataagccatc acactttttt gtcgttaacc aacaagagct
     2401 gtttactgtt aatctgtcag ggagtcacct ccctaacagt agcagagatc agcttttaca
     2461 tatttacgca ttaactgcca cgataggttg aatacccata ttggctgagt aacaaaggca
     2521 catgataatg ctcgttcact ttattaatat gaaattcaac gggaatctca gggaagaaac
     2581 tttcaagatt ttgtttcttg aaatagtccc cggttttaaa tacgacacgg taatcgccag
     2641 tagtcgcagt ttgctcgggc cacagtgcct taattcgtcc atccttatct gttttggcgg
     2701 tattaagttg taaccagccg ttatccgctt tcttttcaag agtgactgtc acgtcggcag
     2761 cgggttttcc tgtttgctgg ttcaaaatgt gcacgctaag aatgttttgt tgtgcggcat
     2821 aaaccaaaga aggtaatgaa aaggctgccg ttgctaccga gagtactaaa taacgcttta
     2881 acatgtttat atccttgtca tgtgaatgag ttttgaggat agcgaaaaaa cgtccagggt
     2941 aaccttacag cagcattaca attttgtaat gaaccaggtt gtttctataa catatgattt
     3001 atggcatatt attttcatga agattctact tattgaagat aatcaaagga cccaggaatg
     3061 ggtaacgcag gggctttccg aagcgggtta tgtcattgat gccgtttctg atggcagaga
     3121 tgggctttat cttgcgctga aggatgatta tgcattgatc attctggata ttatgcttcc
     3181 gggtatggat ggctggcaga tcttacaaac gttaagaacg gcaaagcaaa cccctgttat
     3241 ttgccttact gcaagggatt ctgtcgatga cagagtcaga gggttggaca gtggggcaaa
     3301 tgattatctg gtaaaacctt tttcattttc tgagttgcta gcaagggttc gggcacaatt
     3361 aaggcaacat cacgctctga attcaacatt agaaatcagt ggcttaaaaa tggactctgt
     3421 cagtcaaagt gtcagcaggg acaatatcag tattacactg acgcgcaagg agtttcagtt
     3481 actttggcta ctggcctcca gagctggcga gattataccc agaacggtta ttgcgagtga
     3541 aatttgggga atcaactttg atagtgatac caatacggtg gatgtcgcca ttcgcaggct
     3601 ccgcgcaaaa gttgatgatc cttttcctga aaagctaatc gccacaatcc gggggatggg
     3661 ctattcattc gtagcggtaa aaaaataatg aaaagattat ctataaccgt ccgtttaacc
     3721 ttgcttttta tatttctgca gtctgttgct ggcgctggaa ttgtatggac tctctataat
     3781 ggcctggcaa gtgagttgaa atggcgcgat gatacaacac tcattaaccg gacagcgcag
     3841 atcaagcagt tgttaattga tggggtaaat ccagatacgt tacctgtgta ctttaaccgg
     3901 atgatggatg ttagtcagga tattttgatc attcatagtg atggcatcaa taaaattgtt
     3961 aaccggacaa atgtcagtga tgacatgtta aataacatac ctgctagtga gacaatcagc
     4021 gcagcaggca tttacagaag cattattaat gatacagaga tagatgcttt acgaattaac
     4081 attgatgaag tttcgccatc attaacggtt actgtggcta aattggcttc agccagacat
     4141 aatatgcttg aacagtataa aattaatagc attataattt gcattgtcgc cattatactg
     4201 tgctcagtat taagtccgtt gttaatcagg acggggttgc gagagatcaa aaagttgagt
     4261 ggcgtaacgg aagcgctgaa ttataacgat agccgagtgc cggttgaggt taacgcatta
     4321 ccgagagaac taaaacctct tgggcaggcg ttgaataaaa tgcatcatgc tttagtcaaa
     4381 gattttgagc gccttagtca atttgctgac gatctcgctc atgaacttag aacgccaatt
     4441 aatgcattac tgggtcagaa tcaggttacg ctcagtcaaa tcagaagtat cgctgaatat
     4501 caaaaaacaa ttgccggaaa cattgaagag ctggaaaata tttcgcggtt aacagagaat
     4561 atactgtttc ttgccagggc agacaaaaac aatgttttgg tgaaactgga ttcgctttct
     4621 ctcaataagg aagttgaaaa tcttttggac tatcttgaat atctttcaga cgagaaagag
     4681 atttgcttta aggtcaagtg caatcaacaa atctttgcgg ataaaatttt actgcaacga
     4741 atgttatcga atcttattgt taacgccatt cgatattcac cagaaaaatc gcgtattcat
     4801 ataaccagtt ttcttgatgc caacggctct cttaatattg atatcgccag tcctggaacg
     4861 aaaattaatg agcctgaaaa actcttccgt agattttggc ggggagataa ttcgcgtcat
     4921 tccgtaggtc agggacttgg cctttcttta gtcaaagcga ttgccgaatt acatggagga
     4981 agtgctacgt atcactatct cagtaagcat aacgtgttcc gaattacgtt accgcaaaga
     5041 aattaacatc gggcttcagt acgccataag ttaaccgtga aaaaacgcag ccacgtcgtg
     5101 agtattaacg tggctgcgat tgattatgcg cttacatcac aatgtaacag ggattaactc
     5161 gcgtaagctg ccagcatttc ctgcgccgcc agtttaccca acgcattcgc tgcaaaagga
     5221 ctgtcgccgg tgagacgttt acggtcctta tgtactcgcc cggtgatgtc gtcattaatg
     5281 atgttcatgc ccattttctt caactcttcg ccgaagtacc aggtgagatg acccggcata
     5341 tagccaatat ctggcgtttg tttgtctgcg gcgtctggga atgcgcaaat agaataacca
     5401 ttcagtgggt tatcgctgtg gcgaagtgcc agaaaagccg ccggaccgtg gcaaagggag
     5461 ataacaaaac ggtcattttt gattgcccac tgtaaagcgg cagccacgtc ctggctttct
     5521 ggtaaaccaa taagtgcgcc atgcccacca ggaacaaaga ttgctgcata ttcgctatca
     5581 gcgttgaggc tggcgacaac atccgcgagt ttcttcggat tacggaataa cgatttatgc
     5641 tgttcaaaga atggcattac tttttcatct ttgtgcggca tagcccagta ttcaaacttg
     5701 gtcatcagac cggaaatggt cgccacttcg aattcgaaac ctgcagcatg gagatgatac
     5761 aacggcagca acgtttcaat cggatggtta ccggtcgaga acagttttcc gttatcggtc
     5821 ggcaaataac gttcgtccgc ggcgatcacc agaattttat gtttaccgcg atacggtttt
     5881 ggatagtcca cgccatcaag atcagagacg ggactcgtat attggctaag cgaatattct
     5941 gaagggaaga acgcattatc ttcagcaata tcgacctgcg ggtttttact tgtttgaaca
     6001 gtcatagtgt attccttatt gttgcttagt ta
//
LOCUS       DAZPAB010000211         5982 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZPAB010000211 DAZPAB010000000
VERSION     DAZPAB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.211"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(116..434)
                     /locus_tag="ACHUPG_003570"
                     /pseudo
     CDS             complement(116..434)
                     /locus_tag="ACHUPG_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842854.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(578..3886)
                     /gene="icsA"
                     /locus_tag="ACHUPG_003571"
                     /gene_synonym="virG"
     CDS             complement(578..3886)
                     /gene="icsA"
                     /locus_tag="ACHUPG_003571"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HFR0863023.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4415..5617
                     /gene="virA"
                     /locus_tag="ACHUPG_003572"
     CDS             4415..5617
                     /gene="virA"
                     /locus_tag="ACHUPG_003572"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFR0863024.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5796..>5982
                     /locus_tag="ACHUPG_003573"
     CDS             5796..>5982
                     /locus_tag="ACHUPG_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0863025.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT         1840 a         1355 c         1018 g         1769 t
ORIGIN      
        1 caggctgtac gcttccccat gtgatttgcc tcgccacttc accgggctca ggaccccgta
       61 ggatcttcac cgaaaaaaat gcatcaccag ttgctttatt atttgtttta ggtagacctg
      121 ttactaattt atacctactc tgaccaagtc tggataagaa aggcacacct gcacatttct
      181 acttggaaat gttttctttt accattttgc tcaaaaattt caacatccaa tattcctcgt
      241 acagaactac tcaagtcctg aatagaaaaa gttccagctg gtacagtaga gtcatatatt
      301 actcgacctt gttgagatac aacaacacgt gcattagttt ctgcaatacc agtaatctga
      361 ggcgcataac cacgaatatt tggcggtagc attcgttcat cgctttctaa tgcaattcct
      421 gtgtaacgcc aagcaacagc gcattctggc gcatgatctg gtaaacacgt ttggcattga
      481 tcgcaggcat accatcatgt gcacaaaaac ccaccgagct tttacacggt gggtcccaga
      541 gaaatgcagg acatcaacac gccctgcatt tttattatca gaaggtatat ttcacaccca
      601 aaataccttg ggtgtctctg taactgttat tacccaattg ctgagatacg tttccccaga
      661 tgcttaggtt atctgcaaga cgccccccca caccagtttt gagttcaatc acattacggt
      721 tgcctatctg gtgatttgac atgccattca taattacacc atattgcttt gagctccatt
      781 tccaattcac ttccaaaaat ggttcgaact tacgaagagt ctccttatcg atacaactct
      841 gactaattaa ggatgtacgc aatcccaatt ttgcttgaat attattgccc ccagaggatg
      901 tgaccatagt gccattgtgt tcaatatagt cgtttgcttt taccccatgc ctagttatct
      961 ggacttgtgg attgagatac aatgcgttgt caatattatt atgagcagtc cagcgtatgg
     1021 ttgggtagat atagccagct tccaaagctc ctgttattcc tttgctggaa tatttctcac
     1081 cagttaaccc atctcctttg actgtattat taaaccaact gtactgcaac caagcatcca
     1141 tatatagacc tgttcgttct ttttcatcct gaaaccaaga actgtacaaa ccggcagtat
     1201 aaccgctcac agttcctcgt gaactataac tagtatactt attatgagtt ttgctatcct
     1261 gatatccata agctcccata atacccacat gcaagccatc atgatcagtg aagtttgtct
     1321 tcaaaatatc tccaccaatc tgataaacca tcctgttagc tgtggttttt aattgtctat
     1381 cagctaaatt atgctcatta tgtccaccaa cagtacgaat ccacatggtg gtttcgctga
     1441 gctgacctgt agtaggatca acaatagtgc taccgtgtcg gtcatagaga gaaggactaa
     1501 acatcgtgtt cattgctatc aggttagcaa tataactgcc agcttcaggg cgccatgtgt
     1561 gaatactacc ttcacccaaa ttaagtgaag atacaagctg tgaactagct cggcgactac
     1621 tcatttgagt agactcttga ttgctcatct gttttgattc ttgattatcc atctgactag
     1681 ttagatacca cttattggta ttcagtccag atacagtgcc ctgtttcagg cgatagtcat
     1741 aactaccagc aacaatacgg cctttctgaa taaaagcatt cttatcagaa gaatctgtcg
     1801 aaataatttg aacaccttca agagttttat ctccagagcc tccctcattt gtcactgcaa
     1861 cataggtaat accagacgca tggcctgcaa tttccagccg gtcagtttta ctgttgtcat
     1921 tgccgagaac cgcgcccaaa gataatattc cgccctttcc atgccaatta cctttctgaa
     1981 cataggtctg accgacattt gacgaactat tattgagaat aacatgaccg tttttctcca
     2041 gagtcatgtt acctgtgaca atgacggggt caggcaaaaa aggggcatta atattattga
     2101 ttagcacagt cccactattt aataaagtgt taacagtttg gttcatgcct gaaaaattca
     2161 aggtagcacc tttattaaca ataacaccag cggtacgtgt catagcttca actgtcccca
     2221 tttttagaat gcctgcattg atgttggtga aaccagagta ggtatttttt tccgccaaaa
     2281 tgagagttcc ctcccctgat tttgttaggg attttccgtc ccagctattt atattatgat
     2341 ggctgagatt atctgccaga atagtactaa cagtaagttc agcgttttct ttcagattga
     2401 actctccatg actgtcgcca tctgtatcat tccagctcaa accgtagatc aatttattct
     2461 ggtcactgat atagcttttt gtatagtcaa tagcaccagt agtgagtaac ggattgatga
     2521 tagatatatt agaaacactg aaacctgtta tccctttatc tgcacttagc agtttatagt
     2581 ccttctgcgc ccagttattt ataatagcct ccacttttac attgttgcca aatgacatat
     2641 ttccagaagc ttccagacta ctgattccag ctaaatttaa agaactgttc tcaccaaaag
     2701 aaacgtcacc tgaaacagtg aatttgttac ctgatagtga aacagttgta caatcaccag
     2761 cacataaatc accttcaata gttggtgtgg tcccagtgta atcattgttg ataattttca
     2821 gaatggatga attgttaacc tgtacatcac cagtaatgac agaaccttca tggagtatta
     2881 atgaattgtt tccaccagtg atgtggatag cattacctgc tacattttgt gttccatcat
     2941 cttctttacc tgaaattata tatccgctat tagcaatggt catattgcta ccatttatag
     3001 catcccccca atgtccacca ttaccgccta aaatagctcc attgttgatt acagacaggt
     3061 tatctcctgt gatagcatta ccaccgtagc catcataacc ataaccgtaa gcagcacctc
     3121 cgttgcctcc agcgaacgtg cccttattga ttacagagag gctgcttcct gtgatggcat
     3181 cacctccatt accgccatca ccctcaccat aattgttacc tccattacca ccggaaatag
     3241 tccctttgtt gattatgggt aaattgactc ctgtgacggc atcacctcca ttccccccat
     3301 cactatcact ataactatcg ccaccatgtc cacctgaaat aatttctcca tttattataa
     3361 acagattgtc tcctgtaaca gcctcaccac catcaccgtt atggtcagca ccgctaccgc
     3421 cgctaccacc aagaatcatg ccttgattga ttatagacaa gtcagatccg gtaatagagt
     3481 caccaccatt accgccacaa ctattaccat cattattatc accagcacca ccatgacctg
     3541 cgctaatcat aatatcagag ttaatccgta gcttagttct agatgcatga gaggggttgg
     3601 aagaagttat ataccacgca tccattccat cttcaccagc tcccaaggga gaaagtccat
     3661 caacaggtgt taataatttt tcataattgt cctctgaaaa atgaagttct tgagtacccg
     3721 aaagaggagt agcaaaagct attggccccc cgagcaacag ggatgcacca acaacaaaag
     3781 gagaaaaaga caatttgcat gttttttcct ttaccgtagg taattctccg gcccccccct
     3841 gtgaacattg ggtcatatta caaaaaaatt tgtgaatttg attcatgcac tatattatca
     3901 gtaagtggtt gataaacccc tgaaaagatt ccgttttatc cggaataaag ggacgatata
     3961 tgcaaaaaca tattaaacaa agcctcaacc aaaacatgag agctgtgtta tatatcaatg
     4021 caacgggaga aatttacctg tgtcttgctt tgaaaaaaag agggggaaag ctatatgttc
     4081 gataataaat taataatttt actatgatag tctgcttcag accgcaactt ttcagttctt
     4141 tctgtacgct tgcccaaagt ttaaccactt tgtttttcta tacacccaat ggttctatct
     4201 gccccttgat gttaatcccc aaagccaata acatgaatgt attcatatca caactttcct
     4261 accagactct ccaatgcaat aactgcataa ttgtttttag tggaaatgta tcgttttctt
     4321 aaagagaaga ataacattcc attttattat gttcccaatt taattatatt aatatatatt
     4381 ggcattaata ggaaaataca tcaggagaaa tcaaatgcag acatcaaaca taactaacca
     4441 tgaaagaaat gactcatcat ggatgtcaac tgtcaaatca actacagaag taagttggaa
     4501 taaattgagt ttttgtgaca ttttactgaa aataataact ttcggcatat attctccaca
     4561 cgaaactcta gccgaaaaac atagcgaaaa aaaactaatg gatagtttct caccatcact
     4621 atcacaagat aaaatggacg gagagtttgc ccacgcaaac atagatggca tttcaatacg
     4681 gctatgcctt aacaaaggca tatgttcggt tttttatctt gatggagata aaatacaatc
     4741 cactcaactt tcatcaaaag aatataataa cttgttaagt tcactccccc ccaaacaatt
     4801 caacttagga aaagtacata ctattacagc tccggtatca gggaatttta aaactcacaa
     4861 gcctgcacca gaagttattg aaacagccat taattgctgt acatcgataa taccaaacga
     4921 tgattacttc cacgttaaag acactgattt taatagtgtt tggcatgata tttacagaga
     4981 tattcgagca tctgacagca atagtacaaa aatatatttc aacaatattg aaataccact
     5041 aaaattaatt gcagatctaa ttaatgagtt aggaattaat gaattcattg atagcaaaaa
     5101 agagcttcaa atgctaagtt acaaccaagt aaataaaata atcaattcaa acttccccca
     5161 acaagaccta tgttttcaaa cagaaaaact tttgtttact tcactctttc aagaccctgc
     5221 atttatatct gcactaacat ctgcattctg gcaatctctt cacatcacgt cttcctctgt
     5281 ggaacacata tatgcccaaa ttatgtccga aaacatagaa aacaggctta atttcatgcc
     5341 tgaacaacga gttattaaca attgtggaca tattataaaa atcaacgcag ttgtccctaa
     5401 aaacgacact gcaatctctg cctctggggg gagggcttac gaagttagct catcaatatt
     5461 accatcgcac ataacatgta atggtgttgg tattaataaa atagaaacaa gctatctcgt
     5521 tcatgctgga acacttccaa gcagtgaagg gcttcgcaat gctatccctc cagaaagtcg
     5581 tcaagtatca tttgccatca tatctcctga tgtttaagcc aatattatat tggctgccag
     5641 actgtaaaaa aacgttcttc agaacagctc tataaactat taaaccaaag ctaaaaggat
     5701 ggggaccaat agaatctcag cccatccttt ttatatcatt atgaaccgcc ccggaaatcc
     5761 tggagactaa actccctgag aaagaggtaa acaggatgac taaaaataca cgtttttccc
     5821 ctgaagtccg tcaacgggca gttcgtatgg ttctggaaag tcagggcgaa tatgactcac
     5881 aatgggcagc gatttgttcc attgccccaa agattggctg tatactggaa actctgcgtg
     5941 tctgggtacg ccagcatgag cgggataccg ggggcggtga gg
//
LOCUS       DAZPAB010000212         5857 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZPAB010000212 DAZPAB010000000
VERSION     DAZPAB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5857
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.212"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(76..636)
                     /gene="pagP"
                     /locus_tag="ACHUPG_003574"
     CDS             complement(76..636)
                     /gene="pagP"
                     /locus_tag="ACHUPG_003574"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR0863026.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            1225..2610
                     /gene="dcuC"
                     /locus_tag="ACHUPG_003575"
     CDS             1225..2610
                     /gene="dcuC"
                     /locus_tag="ACHUPG_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR0863027.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(2651..2992)
                     /locus_tag="ACHUPG_003576"
                     /pseudo
     CDS             complement(2651..2992)
                     /locus_tag="ACHUPG_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(3330..3839)
                     /locus_tag="ACHUPG_003577"
     CDS             complement(3330..3839)
                     /locus_tag="ACHUPG_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR0863028.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            4011..4247
                     /locus_tag="ACHUPG_003578"
     CDS             4011..4247
                     /locus_tag="ACHUPG_003578"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0863029.1"
                     /translation="MSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVRPWP
                     KGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            4222..5853
                     /locus_tag="ACHUPG_003579"
                     /pseudo
     CDS             4222..5853
                     /locus_tag="ACHUPG_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1519 a         1292 c         1395 g         1651 t
ORIGIN      
        1 ataaaagtcg cgttattttt gatgttgctt attttagtta ttgattttaa agaagttact
       61 aaaacttcat ttgtctcaaa actgaaagcg catccaggca aagtacacat tgccattgtt
      121 gtaggtaccc ggaatgtagg tcatctgaaa agtcactggg ccataaccca cggaggccaa
      181 tggcagtaga accgggagag ggatgtaatt ccagttatcg cgtgccgtta cgccagcggt
      241 gaatcccaga cctaaatgaa aattttcatc cgccagcggt cgccaggtac tttcccatcc
      301 gtatccggca atcggttccc atttgttcca cgagtcctta aatgccatgg catacaggcc
      361 atgccagttt cctttttcat cccaacgcga caggccaaaa ccgccacccc acggtcgctc
      421 gttatagcga tcggtttttt ctttgtcgta agcgaaacgt gcatgccagg tgatggcagg
      481 aatatataaa tcataatgtt caggctgttg ccaggtttgt gcaatatttt ctctaaacgt
      541 tgtcatcctc tcatctgcgt tagcaaaaac tttaccaacg ctgattaact gaataaaaac
      601 aaaggaaaag atagcgacat atttactcac gttcatttgt gaccataaaa catttatcaa
      661 aaatctacta ctagcatagc aaagctacaa ttaacataac cttaatagac ccaacataaa
      721 gaataatctg aatagctgct ctatttattt ccgcttgttc aagttaaata tttccgcgca
      781 tcgattaaag atgatgcgtc gttttctgcg atgggaatag tcaaaaaaga aaaaccaagt
      841 cttttttgat gacaaaatgc aatcaaggaa aaattatttt atttttttaa cgagatacaa
      901 caatcatctt aacgaagtat ataatatata tcgtcaaccc atgctcatat tagattattt
      961 tttgagcgca cagccgcagc taacaccatt gcaattaaca aatttgcatc aatccaccat
     1021 caatttacac acattattat gtgataattg ccaaccgcta aatatgcgtt ttgttatcta
     1081 tgtataaaaa cagcaacttc aatgtcttaa tggcagtttt tcttgatttt aatcagcatt
     1141 catcgccaat ttattgggca tattttttcc ttaagcttta ggaagttttt attatttact
     1201 ttgggcctgg agacaggaaa aattatgctg acattcattg aactccttat tggggttgtg
     1261 gttattgtgg gtgtggctcg ctacatcatt aaagggtatt ccgccactgg tgtgttattt
     1321 gtcggtggcc tgttattgct gattatcagt gccattatgg ggcacaaagt gttaccgtcc
     1381 agccaggctt caacaggcta cagcgccacg gatatcgttg aatacgttaa aatattacta
     1441 atgagccgcg gcggcgacct cggcatgatg attatgatgc tgtgtggctt tgccgcttac
     1501 atgacccata tcggcgcgaa tgatatggtg gtcaagctgg cgtcaaaacc attgcagtat
     1561 attaactccc cttacctgct gatgattgcc gcctattttg ttgcttgtct gatgtcactg
     1621 gccgtctctt ccgcaaccgg tctgggggtt ttgctgatgg caaccctgtt tccggtgatg
     1681 gtaaacgttg gtatcagtcg tggcgcagca gctgccattt gtgcctcccc ggcggcgatt
     1741 attctcgcac cgacttcagg ggatgtggtg ctggcggcgc aggcttccga aatgtcgctg
     1801 attgacttcg ccttcaaaac gacgctgcct atctcaattg ctgcaattat cggcatggcg
     1861 atcgcccact tcttctggca acgttatctg gataaaaaag agcacatctc tcatgaaatg
     1921 ttagatgtca gtgaaatcac caccactgcc cccgcgtttt atgccatttt gccgttcacg
     1981 ccgatcatcg gtgtactgat ttttgacggt aaatggggtc cgcaattaca catcatcact
     2041 attctggtga tttgtatgct gattgcctcc attctggagt tcctccgcag ctttaatacc
     2101 cagaaagttt tctctggtct ggaagtggct tatcgcggga tggcagatgc gtttgctaac
     2161 gtggtgatgc tgctggttgc cgctggggta ttcgctcagg ggcttagcac catcggcttt
     2221 attcaaagtc tgatttctat cgctacctcg tttggttcgg cgagtatcat cctgatgctg
     2281 gtattggtga ttctgaccat gctggcggca gtcacgaccg gttcaggcaa tgcgccgttt
     2341 tatgcgtttg ttgagatgat cccgaaactg gcgcactctt ccggcattaa cccggcgtat
     2401 ttgactattc cgatgctgca ggcgtcaaac ctgggtcgta ccctatcacc cgtttctggc
     2461 gtagtcgttg cggttgccgg gatggcgaaa atctcaccat ttgaagtcgt aaaacgcacc
     2521 tcggtgccgg tgcttgttgg gctagtgatt gttatcgttg ctacagagct gatggtgcca
     2581 ggaacggcag cagcggtcac aggcaagtaa atagtaatgc cggaggcaag tttcctccgg
     2641 ctttatcatg tcacccactg tggtatatgc gttgtggtct gccaactttg ccgtgaacaa
     2701 tttcagcaat aatcagatgg cggctggcgc aatattcaag ataacgcctg gcagtggtgc
     2761 ggctgatggt cagtgcctgc gccaccgttt ctgccgtatg ttgcacacca ggctctttaa
     2821 acagttttcg caccgcgttt agcgtcaagg ggtcaatgcc ggtcggcagt tcgtctttgg
     2881 gttcaccgcg agcataggca ttaaacatct catcaatttg cttctggctg gcgctatcaa
     2941 tactttccag catatgttta cgctggcgga aacgggttag cgtttgcccc agtgtattga
     3001 ttcacttgaa gtacgaaaaa accgggagaa cattggatta ttcgggatct gatgggatta
     3061 gatttggtgg ggcttgcaag cctgtagtac aaattttagg cgttaaccaa tgaaacgcga
     3121 aagatagtaa aaaattgctt ttgtttcatt gaaaaaacga aaaacaaaaa cactataaat
     3181 catttcaatc atagcttcaa aaaacgttca aaaccgataa caaccaggct gtcatcaaat
     3241 gactcatatc acaaatcagc tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg
     3301 tgaagtatgt tttagctggc tcatggttgt tatacggctt ttttcgcatc ttgtggttcc
     3361 aacaaaggag ctacaggctc ttcctgttta tgaacaccat acattgacaa aatctcttgg
     3421 cgagtttctg gaggtaattt atgcagcgcc aaagctgttt gtagtgtttg gcgatcaata
     3481 cccagcgttg ccaatagggt gtcaacggag atggaggggc gttcttgcat taatccggaa
     3541 agcccatatt tagccatgtt tagctctatc tctcggactt cattttctcc tagtttattg
     3601 attaacaggg agaacatttt caatgtatca gcattcattt cttcgatagc aattttttgc
     3661 atagccggta ttgccgggat atattcaacc ccaggcccgc gacgcccttc ttgtgaacgc
     3721 ttcggccatc cttctttctt tgctcgttta tgcaccccct gtattgtccc aggcattcca
     3781 tccagttcag taatttgttt gggtgtcatc cactggttta tctcatttgc gcttttcata
     3841 aactaagttt accagattta tctatataaa aatcaatgga ttacaaaata aaccacatta
     3901 actgatataa accggtttgg tttatttggt aatgtataaa acagataatc aagacgaatt
     3961 atttgttttg gtaacccttt atcatacgca atatggttgg tgtgcgaaag atgagtaacg
     4021 attggcatcg ggccgacgtg attgcggccc tcaaaaaaac aggaacccct ctggcaaaat
     4081 tgtccagacg ttcaggttat tcaaccggga cgcttgcaaa tgcattagtc aggccatggc
     4141 ctaaaggtga acgcattatt gctgacgcct taaacatgaa acctgaggaa atttggccta
     4201 gtagatatct aagggatcac aatgagagag tggatatcag caattgaatg catgggttta
     4261 tctggtttac ctggttcatt aatgggggtt cacaaaagag ctcagagaga aggatggctc
     4321 aagagaagaa gagaaggaat tagaggggtt gcttacgaat accatatatc ttctcttcct
     4381 aacattgctc gagctgaagt tttgctccaa caaggaaaat ttaaaaccag tcagggatgt
     4441 tttgaaatcg cccgccccac gctggaagct catgattatg atcgtgaggc actatggagc
     4501 aaatgggata aagccagcga ttcccagcgc agcctggctg aaaaatggtt gccctcaatt
     4561 caggccacag acgaaatgct gaaccagggg atttcaacga aaacggcttt tgcgaccgtt
     4621 gcagggcatt accaggtcag cgcatccact ttgcgggaca agtattacca ggtacagaag
     4681 tttgcgaagc ctgactgggc ggctgcactt gttgatggac gtggagcatc ccgtcgcaat
     4741 gttcacaaaa gtgaatttga cgaggatgcc tggcagtttc tgattgcaga ttatctgcga
     4801 ccggaaaaac ccgccttccg caaatgttat gagcgtctgg aactggcagc ccgcgagcat
     4861 ggctggagta ttccctcccg tgccacggcc tttcgccgga ttcagcaact ggacgaggca
     4921 atggttgttg cctgtcgtga aggtgaacat gcactgatgc atctgatacc ggcacagcag
     4981 cgaactgtgg aacacctgga cgccatgcag tggatcaacg gcgacggtta tctgcataac
     5041 gtctttgtac gctggtttaa cggtgatgtg atccgtccga aaacatggtt ctggcaggat
     5101 gtgaaaaccc gaaaaattct gggctggcgc tgcgatgtga gcgagaacat tgattcaatt
     5161 cgcctctcgt tcatggatgt tgtgactcgc tacagtatcc cggaggattt tcacatcacc
     5221 attgataaca cccgtggtgc tgcgaataaa tggctgacgg gaggcgcgcc caatcgctac
     5281 cgctttaagg taaaagagga cgatccaaaa ggactgtttt tactgatggg tgcgaaaatg
     5341 cactggacaa gcgttgttgc cggtaaaggc tggggccagg caaaacctgt tgaacgtgct
     5401 ttcggtgttg gtgggcttga ggaatacgtt gataagcatc cggcactggc tggcgcatat
     5461 acggggccaa atccgcaggc aaaacctgat aactatggcg accacgctgt tgatgcagag
     5521 ctgtttctga aaacctttgc cgaaggtgtg gcgatgttca atgccagaac aggccgtgaa
     5581 acagaaatgt gcgggggcaa actctcgttt gatgatgttt tcgagcgtga atacgccaga
     5641 acgattgtgc gtaagccaac cgaagaacaa aaacggatgc tgttactgcc tgccgaggcg
     5701 gtgaacgttt cacgcaaagg cgagtttacg cttaaagttg gcggctccct taaaggcgcg
     5761 aaaaacgttt attacaacat ggcattaatg aatgccggag tgaaaaaagt tgtggtcaga
     5821 tttgatccac agcagttaca cagcacggtt tattgag
//
LOCUS       DAZPAB010000213         5829 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZPAB010000213 DAZPAB010000000
VERSION     DAZPAB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.213"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            79..258
                     /locus_tag="ACHUPG_003580"
                     /pseudo
     CDS             79..258
                     /locus_tag="ACHUPG_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(574..912)
                     /locus_tag="ACHUPG_003581"
     CDS             complement(574..912)
                     /locus_tag="ACHUPG_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863030.1"
                     /translation="MVAETMFPNIAGSRSCIDCHHVAGRTFSKSARRIRGAPLLRRTS
                     RYDAQTSAFGISNDFRCISYVIPFWVVSSAMNVIPIPSLQTHYRSFITTTNGSAPVSR
                     YVPETGSHVP"
     gene            834..1961
                     /gene="ltrA"
                     /locus_tag="ACHUPG_003582"
     CDS             834..1961
                     /gene="ltrA"
                     /locus_tag="ACHUPG_003582"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR0863031.1"
                     /translation="MFDQQRDGNLYKIWNRLCSGTWFPPPVLEKRIPKSNGKERILGI
                     PTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDALKQCAIRCWRYSWILEVD
                     ISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSCENGELITRTRGTPQGGV
                     ISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRMSDATRLKNRLSERFSEV
                     GLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNFNGERYRKCMPGASNAAM
                     RKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKFWSRNFNYRLWSAMQSRL
                     LKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE"
     gene            2044..2223
                     /locus_tag="ACHUPG_003583"
                     /pseudo
     CDS             2044..2223
                     /locus_tag="ACHUPG_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2250..2735)
                     /locus_tag="ACHUPG_003584"
     CDS             complement(2250..2735)
                     /locus_tag="ACHUPG_003584"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR0863032.1"
                     /translation="MSEQKITGIDLAKTNFYLFSINAHGKPAGKTKLSRNQLLNWLVQ
                     QPKMTVAMEAGGASHYWAREIRKLDHDVILLPAQHVKAYQRCQKNDYNDAQAIAEACQ
                     HGTIRPVPIKTLEQQDVQTFLNMRRLVSMERTQLINHIRGLLAEYGIVFSKGAAELRQ
                     K"
     gene            complement(2991..3971)
                     /locus_tag="ACHUPG_003585"
     CDS             complement(2991..3971)
                     /locus_tag="ACHUPG_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFR0863033.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3971..5170)
                     /locus_tag="ACHUPG_003586"
     CDS             complement(3971..5170)
                     /locus_tag="ACHUPG_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0863034.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5344..5478)
                     /locus_tag="ACHUPG_003587"
                     /pseudo
     CDS             complement(5344..5478)
                     /locus_tag="ACHUPG_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5492..>5829)
                     /locus_tag="ACHUPG_003588"
     CDS             complement(5492..>5829)
                     /locus_tag="ACHUPG_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR0863035.1"
                     /translation="HWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTS
                     DDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIG
                     SFIEKHMFY"
BASE COUNT         1597 a         1339 c         1389 g         1504 t
ORIGIN      
        1 gcttattatg cttccatcgg aaacgatgat ctggcagcct gagttcacag ataaaacact
       61 ctccaggaaa cccggggcgg ttcagttgct gaactgctgg aaccgctgta tgacatcctg
      121 cgccagtatg tgctgatgcc cggtaaagtc catgccgatg atatccccgt tccggtccag
      181 gagccgggca gcggtaaaac aaggactgcc cggctgtggg tctacgttcg tgatgaccgt
      241 aacgccggtt cggaaattgc agcataagtt atccatgctc tgaggtgggt agaggtgggc
      301 cctccggtca gggtttacag gagcacagac caaacgaccc ggtgctgctt cattctaagg
      361 aatgaggtgg tgagcgactc gggaaaaggc agagatctac tgtcacgtgg gttcgggaag
      421 ctgaacgaaa gtaaactgtc cgatgaagca tcgaaatctt agatgatgcc aaaatggggc
      481 cgtacagccg tcccctgata agcgcagaag acacctgctt actgactgcg cggcatccgg
      541 tgtgaaggca gcaggatccc gggccagacc atctcaggga acgtgagaac ctgtttccgg
      601 cacgtatcgg gaaacagggg cggacccatt cgtagtagtg atgaaagatc tgtaatgggt
      661 ctggagcgaa gggatgggga tcacattcat tgcagaagac acaacccaaa aggggatgac
      721 gtaactgatg caacgaaaat catttgaaat accaaaagca ctggtctggg catcatatct
      781 ggatgtgcgt cgcaacaagg gagccccccg gatgcgacgg gcagactttg aaaatgttcg
      841 accagcaacg tgatggcaat ctatacaaga tctggaaccg gctatgttcg ggaacatggt
      901 ttccgccacc agtgctggaa aaacggatcc cgaagtcgaa tggcaaggaa cgcattctgg
      961 ggatcccaac agtatccgat cgaattgctc agggagcgat aaaacttttc atggaagaaa
     1021 agcttgaccc gattttccat gctgattcat atggttaccg accaggcaaa tcagctcatg
     1081 acgctctgaa acaatgtgcc atccggtgct ggcgttatag ctggatactg gaagtggaca
     1141 tcagtgcgtt tttcgaccat gtgagacatg atctggtgct caaagctctg gaacaccacg
     1201 ggatgcctaa atgggtcatc ctgtactgtc gacgctggat ggaagcgcca atgcagagtt
     1261 gtgaaaatgg agaattaata acccgaacac gaggcactcc gcagggcggg gtcattagtc
     1321 cgctacttgc taacttgttc cttcactatg catttgattt gtggatggaa agagaatatc
     1381 ggggggtacc gtttgagagg tacgctgacg atattgtagt acattgttca cgaatgagtg
     1441 atgcaacaag gctaaagaac aggttatcgg agcgcttctc ggaagtcggg ctggtcctga
     1501 atgcagggaa aacgaatatc gcctacattg acacgtttaa aaggagaaac gtcgcaacga
     1561 gtttcacctt tctcggatat gacttcaaag tgcgtacgct gaagaatttc aacggcgaac
     1621 ggtaccgaaa atgcatgccg ggtgcgtcaa atgcagcaat gcgcaaaata acagaaacaa
     1681 tcaagaagtg gcgtatacat cgctcaacag ctgagagttt gctggatttt gcgaggcgct
     1741 acaatgcgat agtgagaggc tggatcgagt actacggaaa gttctggtcc agaaatttca
     1801 actatcgact gtggagtgca atgcagtcac gtctgctcaa gtggatgcag tctaaataca
     1861 gactttcgaa ccggaaggct cagcgaaagc tgacgctggt aaggaaggag tatccgaagc
     1921 tatttgttca ctggtattta ctacgtgcat caaatgagtg atcaagagcc gtatgacgcg
     1981 agagtgtcac gtacggttcc gtgagcagcc ggggagtgaa attctctccg gctgactcga
     2041 ctcgcgggtt ccgacagcga tgacgaacat gcggcggtgt tgtactcgct gatcggcaca
     2101 tgccgtctga acaatgtgga accagaaaaa tggctgcgtt acgtcattga gcatatccag
     2161 gactggccgg caaaccgggt acgtgatctg ttgccctgga aagttgatct gagctctcag
     2221 taaatatcaa tacggttctg acgagccgct tacttttggc gtaattctgc tgcgccttta
     2281 ctgaacacaa tgccgtattc agcaagaagt ccacgaatgt gatttatcag ttgtgtcctt
     2341 tccattgaaa ccaaccgacg catattcaaa aaagtctgga cgtcctgttg ctccagagtt
     2401 tttattggta cgggtcgtat agttccatgt tggcaggcct cagctattgc ctgtgcgtcg
     2461 ttataatcat ttttctggca ccgctgataa gctttaacat gttgtgccgg aagcaatatt
     2521 acatcatggt cgagtttcct gatttcccgg gcccagtaat gagatgcccc gccagcctcc
     2581 atcgcaacag tcattttagg ctgttgcacc agccagttaa gcagttgatt gcgagaaagt
     2641 ttggtctttc ctgcgggttt gccatgagca ttgatactga aaagataaaa gttcgtttta
     2701 gccagatcaa tgccggtgat tttctgttca gacatggtat cccgcttact tttgttgtta
     2761 aagtttatcc atcatggcgc aattgaaggc tgccgtgaaa gagggggaag actatctcat
     2821 cggttactgt taaacggcta agagaaacta tctttttcaa gcaaagaaga tacggtgaaa
     2881 caggcgattt caccgtgaaa tactgttcaa aaaaaacgct aataaaagca aaatttcttt
     2941 taaaacagcc aattaaaaac tggcgagttg ctggcgaaaa acttgaattt tcacagcgaa
     3001 ttcagattat cttttagtac aagagcaatc atacgatcta acttatcctg aacttccaac
     3061 ggtaatctgc cgaattcata tgagaacgta cgtcccttta ctcgttttcg agcaaattta
     3121 tctttactat caaatttcca taacaaagtt acaaccgctt tatcctttac tcctgaatca
     3181 agcaatgaag acatttcctt ggttataagc cgcataatac tgtttttaac ctcgtcttca
     3241 gaaagattgt catcagcagt aataagctca atactcggcg agatattctt cacaacttca
     3301 tcaatagata tatcacccag cctcagtcgt tcacttaatt cagccagttg cctgtaatcg
     3361 gcgtatgtca gctcagactg aacaggaaaa agagacacaa aatctttagg tacggacgct
     3421 gcttggaggg cacgagtcac cttagctgcc gacaatccct ctctttccgc aatttgcttc
     3481 tggttcatac cggcctcttt gagacgtact aaacgcaacc caatttcacg aatattatgt
     3541 tcaagtgacg tctgcaaatc tttcgccaga tgctgagctt cagaaacagt aagctcatca
     3601 tccgttacaa gtacacgtaa tccaaccttg catagcaaag ctgctgcccg acgacgggaa
     3661 ccatccaaaa tctcaataag atcgccagtc cgaatcccaa tacatggata gaattgttgt
     3721 aaacgtatag ttctggtgat gtcttttagt gagtccggcg tcagggctgt ctgttcgcgg
     3781 ccattcacct cttcaaccac aaaagtttga gtatccactt tgtcatgatc aaccttgatc
     3841 tcagtaaatt ttgcctttcg gccagtattg agggtaaata catgaactgg agccgatatt
     3901 tcttcagtat tattcaggat atttgtattc agcgtacggc caatagtcgg tcggtgcttt
     3961 ctgttttcca tcagtagttg gcccttataa attcaatacg atcaaaaact gctttagcga
     4021 aatcttctgc tgccatacgt gcatttttca aagcttcacc actgccaaca taagtaacag
     4081 gattcgcaga tatcacggta tcaaacgact cgccgcttct ctcaaaacca tcaagacgtg
     4141 gcattgaaac atcaagcatg tcaccaccga aaatctcttt tgtcaggcta tggcagtatt
     4201 tatgatctga cttattcgcc agtttcgaca tgaagccaat atttgcctgc aaacggcatg
     4261 aacatccatc ctgttcaata atctgtacaa gttcaggtaa acgagccaaa tatttaagcg
     4321 tggaatggaa atcaacttgt gctggcggca ccggtgtaaa catgatatct gcggcagcaa
     4381 tggcattttt caaaaatgcg tccagatgcg ggcctgtatc aataaggatg aagtcgaaat
     4441 catgcttcag cttatcaata atattctcac gcaatatcgc gtgtttgttt tgtcctaata
     4501 gatgctcctc gcacagagta tcccagtttg atgcaataaa ggcgtcgtca atagaagccg
     4561 gcataacata aacgccaggg ataactgacg gaacgataaa atcttccagt aattcctcac
     4621 gactcacgtt ctgcaacata gcctgcggtg cggttgtatc aaccagcccc accgcatgca
     4681 agtagttcag aaacatagtg gctgatgact ggggatctaa atcaaggagc agaatacgta
     4741 aatcttctga cagcaggtga ggatgagcac gcagggcgtg agctacggaa acactactca
     4801 cggttttaga cacaccgcct tttagactac cgatgaatat ggaatatgcc tcactataac
     4861 gatcgcgata tttaggaatg ccacgatgtg catagatatc gataatgttc tggatggtta
     4921 atgcatattt tgtcgcggtt cctgctgggc gtttttcaaa ctgatagccc tgagcctcca
     4981 tctcaccgac tgcaccatcc acgcttgcgc gcgttaattt tggaagctta gctacagcag
     5041 actttgaata gacttgataa aaaacatcag cctgaaactc ctgcttttgc tcctgaatct
     5101 gttttgttag ggcgagcaac attttatctg cacgctgcgc aaccttactt aactgctcaa
     5161 aactagtcat tccccctccg tgctatgtag gattttaact tattacaaaa aaaactgcat
     5221 tagcagggta atgcatgcaa gtacatattg tacatgctta tattaaaaat gcgcgcattg
     5281 ctgatttatt aatcatcgga atgaatatta ctgtaagata gtatgccttt ccgggagaaa
     5341 gacgctgatt tcccccgaat aatccagttc accaccacac tccggacaac agctttcttc
     5401 aggctccaga tggtgtattt cgcgggaaac atgttccggt aacggacggc gatgacgtga
     5461 ctgtcgcaac aggcgggggt agtgcttcca atcagtagaa catgtgcttt tcaatgaagg
     5521 agccgatgac tttttcgtgg atttccacgg aacgcgagaa gcagattgtt ttacgtgcca
     5581 gccgcttgat gcgggtgcgg agggtccggt tgttacgtgc aatacgttgc gtgaagattt
     5641 tgccggtcag gtgtttctcc ttcggcactt ctctggcgta gctgccccag tcatcgctgg
     5701 tcaccatgcc gatacagaac ggagtcaaca gggctaacag ttcccgacag gtctcatcat
     5761 tccgtggccc gaaagtgtaa gccaacacac cgccagtttt agtgttgtac gcataccaga
     5821 gccagtgtt
//
LOCUS       DAZPAB010000214         5804 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZPAB010000214 DAZPAB010000000
VERSION     DAZPAB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.214"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            182..1090
                     /locus_tag="ACHUPG_003589"
     CDS             182..1090
                     /locus_tag="ACHUPG_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFR0863036.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1136..3127)
                     /gene="tkt"
                     /locus_tag="ACHUPG_003590"
     CDS             complement(1136..3127)
                     /gene="tkt"
                     /locus_tag="ACHUPG_003590"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR0863037.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3405..4163
                     /gene="loiP"
                     /locus_tag="ACHUPG_003591"
                     /gene_synonym="yggG"
     CDS             3405..4163
                     /gene="loiP"
                     /locus_tag="ACHUPG_003591"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR0863038.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4369..5289)
                     /gene="speB"
                     /locus_tag="ACHUPG_003592"
     CDS             complement(4369..5289)
                     /gene="speB"
                     /locus_tag="ACHUPG_003592"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR0863039.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5425..>5804)
                     /locus_tag="ACHUPG_003593"
     CDS             complement(5425..>5804)
                     /locus_tag="ACHUPG_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863040.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1393 a         1537 c         1524 g         1350 t
ORIGIN      
        1 tttgtttgtt attgtatttg tgatgcatat ctctatttat ttattgaatg ttcgaaaaca
       61 tacttttcta atgcaatgtt ttttcgctat acccctttat agattctgaa ttctcacctg
      121 tcggctgttt tctcctcatc tcatgtgaca tattcccata ctttactcaa ggaagattgt
      181 catgaaagca cctgtttcat taacctcatt tcgcccacaa aaatctcttg ctatcgccat
      241 tggcgtgctt gccgtggttg tgctgccatt tctctcgtat tacaccgtta atgaaggtga
      301 acgcggcatt ttgctgcgct acggaaaaat cgtaaaagtt gccgaaccgg ggctgggttt
      361 taagatccct tttatggaaa gcgtggagaa aatttccacg cgtaatcagg ctgtggtcta
      421 tcagggatta caggcctaca gccgcgacca gcaaccggca caaatgacag tatcagtcag
      481 cttccacatt aaaccttcag aagcaggcgc ggtttatacc acctacaaca ctattgaagc
      541 attaaaagat cgtctaattg tccgtcagtt gccaactcag ctggagaaca ttttcggcca
      601 atataccgcc atcagcgccg tacaggatcg tacaaaattg gttcaggatc tgcaaaatgc
      661 catgcgcaaa gccgttgttg ggccagtggt aattgacggg gtccagattg aaaatattga
      721 tttttctgac gcttatgaaa agtcgattga aaaccgaatg aaagcagaag tcgccattgc
      781 cacgcgtaag caaaatctgg aaacagagaa aatccaggcg caaatagcgg taactcaggc
      841 ccaggcagaa gcagacagta aactggcggc agccaaagca gaagcggaaa ctattcgcgt
      901 aagaggcgcg gcagaggctg aaactattcg tttaaaaagt gcggctgaag cagaagcaat
      961 ccgcctgcgc ggtgaagctc tgcgggataa tcccggactt gtagccctca ccactgccga
     1021 acgttgggac ggtaaactgc ccgataccat gatccccggc agcacagttc cgtttatctc
     1081 tactaagtaa aaaaagggcc gctttcgcgg tccttctcac aaaacgtctt actgattaca
     1141 gcagcgcttt tgctttcgca acaacgttat caacagtgaa gccgaactct tcaaacagca
     1201 gctctgccgg agcagattca ccgaaggtgg tcatgccgac gatagcgccg ttcaggccaa
     1261 catacttgta ccagtagtca gcaatacccg cttctaccgc aacgcgtgca gtaaccgctt
     1321 tcggcagtac ggattcacgg taagccgcat cctgcttgtc aaatgcgtcg gtagacggca
     1381 tggacaccac gcgcgctttc acgccttcgg cagtcagttt ttcgtaggca gcaaccgcca
     1441 gttcaacttc agaaccggtg gcgatgaaga tcagttccgg ctgaccagca cagtctttca
     1501 gcacataacc accgcgcgcg atgtttgcca gctgctcttc agtacgttcc tgctgcgcca
     1561 ggttctgacg ggagaggata agcgcagtcg ggccgtcctg acgctcaacg ccgtatttcc
     1621 acgcgaccgc ggattcaacc tggtcacacg gacgccatgt agacatgttc ggggtcacgc
     1681 gcagagaagc gacctgctct accggctggt gagtcgggcc atcttcgccc agaccgatgg
     1741 agtcgtgggt gtaaaccatc acctggcgct gtttcatcag cgcagccata cgtacggcgt
     1801 tacgtgcgta ttccacgaac atcaggaagg tggaggtgta cggcaggaag ccaccgtgca
     1861 gggagatacc gttagcaatc gcggtcatac cgaactcgcg aacaccgtag tggatgtagt
     1921 tacctgcagc atcttcgttg attgctttag aaccagacca cagggtcagg ttagacggtg
     1981 ccaggtcagc agagccgccg aggaattcag gcaacagcgg gccgaacgct tcgatagcat
     2041 tctgcgacgc tttacggctg gcgattttcg ccggattagc ctgcagttta gcgataaact
     2101 ctttcgcttt ggcgtcgaag tcagacggca tttcgccttt catacggcgg gtaaattcag
     2161 ccgcttcctg cggataagct ttcgcgtaag ccgcaaactt ctcattccat gcagattctt
     2221 tcgcctggcc tgcttctttc gcatcccact gagcatagat ttcagacggg atttcgaacg
     2281 gtgcatattt ccagcccagc tgttcgcggg tcagggcaat ttcagcgtcg cccagcggcg
     2341 caccgtggga gtcgtgagta ccggctttgt tcggggaacc gaaaccgatg atggttttgc
     2401 acatcagcag ggacggtttg tcagtcactg cgcgcgcttc ttcaactgcg cgtttgatgg
     2461 atgccgcgtc atgaccgtcg atgtcgcgaa taacgtgcca gccgtaagct tcgaaacgca
     2521 ttgctgtgtc gtcggtgaac cagccttcaa cgtgaccatc gatagaaata ccgttgtcat
     2581 cgtagaacgc aatcagttta cccagcttca gcgtacccgc cagagagcaa acttcgtggg
     2641 agatgccttc catcatgcag ccgtcgccca tgaaggcgta ggtgtagtgg tcgacaatgt
     2701 cgtggcccgg acggttaaac tgcgccgcca gcgttttttc tgcaatcgcc ataccgactg
     2761 cgttggcaat accctgaccc agcggaccgg tggtggtttc cacaccagcg gtataaccta
     2821 cttccggatg gcccggagtt ttagagtgca gctgacggaa gtttttcagt tcttccatcg
     2881 gcagatcgta accggtgagg tgcagcaggc tgtagatcag catggagccg tggccgttgg
     2941 acagcacgaa gcggtcacgg tcagcccagg acggattctg cgggttgtgt ttcaggaaat
     3001 cacgccacag gacttcggca atgtcagcca tacccatagg ggcacccggg tgaccggatt
     3061 tggctttctg tactgcgtcc atgctcagcg cacgaatagc attggcaagc tctttacgtg
     3121 aggacatttt gactccagat cggatgatga agggcacgcc cttaacgact tgacgacagc
     3181 gcgttttggg ctacgccgga aaatttgcca acaatttacc gcaagccgcg cgtcatgtac
     3241 atggaacatc cttttgccgc ttcagaaatc tctggatcat gctcgcatgt tgcgcaatct
     3301 gctcgcccgt ccgctgcgct tttccttata ctgagactga gcgtcgattc acctgcaaac
     3361 ggcgcatttt tagaataatc ctgaccttgt gcggaagaga aaacatgaaa attcgcgcct
     3421 tattggtagc aatgagcgtg gcaacggtac tgaccggttg ccagaatatg gactccaacg
     3481 gactgctctc atcaggagcg gaagcttttc aggcttacag tttgagtgat gcgcaggtga
     3541 aaaccctgag cgatcaggca tgtcaggaga tggacagcaa ggcgacgatt gcgccagcca
     3601 atagcgaata cgctaaacgt ctgacaacta ttgccaatgc gctaggcaac aatatcaacg
     3661 gtcagccggt gaattacaaa gtgtatatgg cgaaggatgt gaacgccttt gcaatggcta
     3721 acggctgtat ccgcgtctat agcgggctga tggatatgat gacagataac gaagtcgaag
     3781 cggtgatcgg tcacgagatg gggcacgtgg cgttaggcca tgtgaagaaa gggatgcagg
     3841 tggcgcttgg tacaaatgcc gtgcgagtag ctgcggcctc tgcgggcggg attgtcggca
     3901 gtttatctca atcacaactt ggtgatctgg gcgagaaatt agtcaattcg caattctccc
     3961 agcgccagga agcagaagcc gatgattatt cttacgatct tctgcaccaa cgcggcatca
     4021 gcccggcagg tcttgccacc agctttgaaa aactggcgaa gctggaagaa ggtcgtcaaa
     4081 gctcaatgtt tgacgaccat cctgcatccg ccgaacgtgc ccagcatatt cgcgatcgca
     4141 tcagcgcgga tgggattaag taaagcctag tggtgtcgaa cgtaggtcag ataaggcgtt
     4201 cacgccgcat ctgacattaa tggcacattt gttaccttgt gcgcattgcc ggatgcgatg
     4261 ctggcgcatc ttatccggcc tacgggtgcc gaacgtaggt cggataaggc gttcacgccg
     4321 catccgacat taatggcacg ttttacccgt gcgcatcgca tctggtgctt actcgccctt
     4381 tttcgccgcc tgaatataca gcatctccag cgccagcgtc gccgcagcca gagcggtgat
     4441 ttccgactga tcgtatgccg gagccacttc cactacgtcc atcccaacga tgttgagatc
     4501 tttcaggccg cgtaccagtt taatggcgcg atcggaggtc aggccgccaa tcactggcgt
     4561 accggttccc ggcgcgaaag caggatccag gcagtcgata tcaaaagtca ggtaaaccgg
     4621 catatcaccc acaatctgtt tcacctgggc gataacgtca tccacgctgc gatcgttcac
     4681 ctggcaggcg tccagcacgg taaagccgtt gtctttatca aactcggtac gaataccaat
     4741 ctgcacggaa tgattcgggt cgatcagacc ttctttcggc gcggtataga acatagtgcc
     4801 gtggtcaaat tcacaaccgt tcgcataggt atcggtgtgg gcgtcaaagt gtaccagcgc
     4861 cattttgccg aaatgcttcg catgagcacg cagcagcggc agcgtaacaa agtggtcacc
     4921 accgaaggag agcatacgct taccggcagc cagcagcttc tcggcgtgcg cctgcagctt
     4981 ttcgctcatc tcacgggcat cgccaaaggc atataccaga tcgccgcagt ccacgacgtt
     5041 cagacgctca cgcatgtcga aattccacgg gaagcggttg tgttcccagg ccagattcgt
     5101 cgaaacctga cggatcgctg ccggaccgtg gcgaccaccc gcacgaccag aagtggccat
     5161 atcgaacggc acgccagtaa tcacccagtc tgcatcgctg tcatacggct ggaagttcat
     5221 cggcaggcgt aaaaaaccaa aggcattgga aaccagtgag ttatcgtatt gatgacctaa
     5281 ggtgctcatg gatggactcc tattacaaag tcgatataaa aaaacccttc cgcgtcgtta
     5341 ggcccgacga ggaagggttg gatttgcgaa acacaagttt gtgctaatta tcgccttaaa
     5401 tgggcgaggc atcaagcgag gttgctattt ccgacacggt ggcggcgcat agccattgtt
     5461 atctgccgga aaggcttgtc cagagcacgg cggcagcgcg ttttgcgccg ggccttgtaa
     5521 ttcaaaggcg gcaaagccga ttaccccggt attacgcaca tcgccttgcg ccgaattggc
     5581 tgcgtaggaa tcagcaacgt tggcgaactg aaaagccgct tcagtatgtt tatctttgcg
     5641 gaatccttta atcactaacg aatctccggc attcacaata tagccgttgt tattcagcga
     5701 accttgcttg ccgttcagca catccaggcc atcagccgta gcaacgatct cgtagttggt
     5761 gttacgactg taattacgga catataattg ataattcatt ccga
//
LOCUS       DAZPAB010000215         5797 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZPAB010000215 DAZPAB010000000
VERSION     DAZPAB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.215"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..948
                     /gene="lpfD-O113"
                     /locus_tag="ACHUPG_003594"
     CDS             <1..948
                     /gene="lpfD-O113"
                     /locus_tag="ACHUPG_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR0863041.1"
                     /translation="SGYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIY
                     YTSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKY
                     NCNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMAD
                     VYIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLG
                     ILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTV
                     YGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
     gene            1196..2236
                     /gene="pstS"
                     /locus_tag="ACHUPG_003595"
     CDS             1196..2236
                     /gene="pstS"
                     /locus_tag="ACHUPG_003595"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR0863042.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            2323..3282
                     /gene="pstC"
                     /locus_tag="ACHUPG_003596"
     CDS             2323..3282
                     /gene="pstC"
                     /locus_tag="ACHUPG_003596"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR0863043.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            3282..4172
                     /gene="pstA"
                     /locus_tag="ACHUPG_003597"
     CDS             3282..4172
                     /gene="pstA"
                     /locus_tag="ACHUPG_003597"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR0863044.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            4264..5037
                     /gene="pstB"
                     /locus_tag="ACHUPG_003598"
     CDS             4264..5037
                     /gene="pstB"
                     /locus_tag="ACHUPG_003598"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR0863045.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            5052..5777
                     /gene="phoU"
                     /locus_tag="ACHUPG_003599"
     CDS             5052..5777
                     /gene="phoU"
                     /locus_tag="ACHUPG_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR0863046.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1435 a         1397 c         1516 g         1449 t
ORIGIN      
        1 tcgggatatc ttattaccgc gaacgaaaat aaggttggaa cgacatttaa caccactgta
       61 actaatggat cctcatatct tggacgctgt cattgtgata ctggtaacgt aggagaattc
      121 ccctatatct attatacgtc aaaaataaac caggcattaa cttatgctgg ggttcactct
      181 aatattaatt attatgatct taatccgaac cttgatgttg ggattgctat agatattctt
      241 ggggttgggt atgtgaatgc accttttgaa tatcatgcta acaacccgtc tggtaataca
      301 aagtacaatt gcaaccgcat tgaaccttta agtatatcca gtggggctaa agcgatagta
      361 tatttttata tcaagaagac atttgcagga aaattgatta ttcctgaaac aaagatagtg
      421 acattgtatg gaacaattag ccgtgacacc ccggtggatt actcacagcc gatggctgat
      481 gtttatattc ggggcgatat tacagctccg caaagttgcg aaataaataa tttaaagcca
      541 gtttgttttg attttaaaga aataccagct gcagattttt catctgttgt tggaagtgcg
      601 gtaacaacac ataaaattac taaaacagtc actattgagt gtgaaaacct ggggatactg
      661 aataccgatg atatcagtac gtctttttat gctaccgaac ccaatacgga caattcaatg
      721 gtcgtaactt caaactctaa cgtggggata aaaatttacg acaaaaataa taaggaaatc
      781 aaagtgaacg gtggtgagtt gccaacagac atggataaat caacagtgta tggtgaaaaa
      841 tcaggtagtg taactttttc agctgctcct gcgagcctta ctggagctcg cccagccccg
      901 gggcaattta ctgcaacagc gacgataact gttgagattg tgcgctaata ggtctgacaa
      961 cgattgttca tcacatcgtt gtcatctcac ccatttcttt atcactttta aatgcatcat
     1021 gacaaaaaga tggcatgtgg aattttcatc ttcacattta aaatcagtaa gttatttggt
     1081 aattcctttc tgtcataaaa atgtcatatt ccttatatat aactgtcacc tgtttgtcct
     1141 attttgcttc tcgtagccaa caaacaatgc tttatgaatc ctcccaggag acattatgaa
     1201 agttatgcgt accaccgtcg caactgttgt cgccgcgacc ttatcgatga gtgctttctc
     1261 tgtgtttgca gaagcaagcc tgacaggtgc aggtgcaacc ttccctgcgc cggtgtatgc
     1321 caaatgggct gacacttacc agaaagaaac cggtaataaa gttaactacc agggtatcgg
     1381 ttcttccggt ggcgtaaaac agattatcgc taataccgtt gattttggtg cctctgacgc
     1441 gccgctgtct gacgaaaaac tggctcagga aggtctgttc cagttcccga ccgtgattgg
     1501 cggcgtggtg ctggcggtta acattccagg gctgaagtct ggcgaactgg tgctggatgg
     1561 taaaaccctc ggcgacatct acctgggcaa aatcaagaag tgggatgatg aagccatcgc
     1621 caaactgaat ccgggtctga aactgccttc acaaaacatt gctgtagtac gccgcgcaga
     1681 tggctccggg acttccttcg tcttcaccag ctacctggcg aaagtgaacg aagagtggaa
     1741 aaacaacgtt ggtactggct ctaccgtaaa atggccgatc ggtctgggcg gtaaaggtaa
     1801 cgacggtatc gccgcgttcg ttcagcgtct gccgggtgca attggttatg ttgaatatgc
     1861 ttacgcgaag cagaacaacc tggcgtacac caaactgatc tccgctgatg gtaaaccggt
     1921 tagtccgacc gaagaaaact tcgctaatgc agcaaaaggt gcagactgga gcaaaacctt
     1981 cgctcaggat ctgaccaacc agaaaggcga agatgcatgg cctattacct ctaccacgtt
     2041 cattctgatc cacaaagatc agaagaaacc agaacaaggc acagaggtgc tgaaattctt
     2101 cgactgggcg tacaaaaccg gggctaaaca ggcgaacgac ctggattacg ccagcctgcc
     2161 ggatagtgta gttgaacagg ttcgcgctgc gtggaagacc aatattaaag acagtagcgg
     2221 taagccgctg tactaataaa actccaggcc gggtacggtg ttttacgccg catccggcat
     2281 tacaaaatga ctttgtaaac gcgtttaact gaagagtaac ttatggctgc aaccaagccc
     2341 gcttttaacc caccgggtaa aaagggcgac ataattttca gcgtgctggt aaaactggcg
     2401 gcgctgattg tgctattgat gttgggtggc attattgtct ctctgatcat ctcctcctgg
     2461 ccgagcattc agaaatttgg tctggctttc ctatggacca aagagtggga tgcaccgaac
     2521 gatatctacg gggcgctggt gccgatctac ggtacgttgg tgacttcgtt tatcgcgctg
     2581 ctgatcgccg tcccggtgag tttcggtatc gccctgttcc tgactgagct tgcgcctggc
     2641 tggctgaaac gcccgctggg tatcgccatt gagctgctgg cagccattcc aagtatcgtt
     2701 tacggcatgt ggggcctgtt tatctttgcg ccgctgttcg ccgtttactt tcaggagccg
     2761 gtcggcaata tcatgtcgaa tatcccgatt gttggcgcgc tgttctctgg ccccgcattt
     2821 ggtatcggta tcctcgcggc aggcgtgatc ctcgccatca tgattattcc gtacattgcg
     2881 gcggtaatgc gtgatgtgtt cgaacaaacc ccggtgatga tgaaagagtc ggcctacggt
     2941 attggctgca ccacctggga agttatctgg cgtatcgttc ttccgttcac caaaaatggt
     3001 gttatcggcg gcatcatgct ggggctgggc cgcgcgctcg gtgaaaccat ggcggtgacc
     3061 tttatcatcg gtaacaccta ccagctcgac agcgcctcgc tgtacatgcc gggcaatagt
     3121 atcacctctg cgctggcgaa cgaatttgcg gaagcggaat ccggtctgca cgttgccgca
     3181 ctgatggaac tgggcctgat cctgtttgtg attaccttca tcgtcctcgc cgcatcgaag
     3241 tttatgatta tgcgcctggc taagaatgag ggggcacgct aatggctatg gttgaaatgc
     3301 aaaccactgc ggcgctggct gaatctcgcc gcaaaatgca ggcgcgtcgc cgcctcaaaa
     3361 accgtattgc gctgacgctc tcgatggcga cgatggcctt cggcctgttc tggctgatct
     3421 ggattttaat gtccaccatc actcgcggta tcgacggtat gtcgctggcg ctgttcactg
     3481 aaatgacgcc gccgcccaat acggaaggtg gtggtctggc gaacgccctg gcgggtagcg
     3541 ggctgttaat tttgtgggcc acggtattcg gtacgccgct gggcattatg gcggggattt
     3601 atctggcgga atatggtcgt aaatcctggc tggcagaagt gattcgcttc attaacgaca
     3661 ttctgctctc cgcgccgtcg attgtggttg gtctgtttgt ctacaccatc gtggtggcac
     3721 agatggagca cttctccggc tgggcgggcg taattgcgct ggcgctgttg caggtgccga
     3781 tcgttatccg caccactgag aacatgctga aactggtgcc ggacagcctg cgtgaagcgg
     3841 cttatgcgct gggtacaccg aagtggaaga tgatctccgc gattacgctg aaagcgtcgg
     3901 tgtccgggat tatgaccggt atcctgctgg cgattgcccg tattgccggt gaaaccgcgc
     3961 cgctgctgtt taccgcgctc tccaaccagt tctggagcac ggacatgatg cagccgatcg
     4021 ccaacctgcc ggtgacgatc tttaagtttg cgatgagccc gtttgcggaa tggcaacaac
     4081 tggcctgggc cggggtattg atcattaccc tgtgcgtcct gctgctgaac attctggcgc
     4141 gcgttgtttt tgcgaagaat aaacacggtt aacacttttc cggatgcggc gtgaacgcct
     4201 tacccggccc tacggtaagc ctgattagcg aaaagcatca ggtacgatga ggaaaagatt
     4261 gcaatgagta tggttgaaac tgccccgagt aaaattcagg ttcgtaattt gaacttctac
     4321 tacggcaaat tccatgccct gaaaaacatc aacctggata tcgccaaaaa ccaggttacg
     4381 gcgtttatcg ggccgtccgg ctgcggtaaa tcgacgctgc tgcgtacctt caacaaaatg
     4441 tttgaactgt acccggagca gcgtgcggaa ggtgaaattc tgcttgatgg cgacaacatc
     4501 ctgaccaact ctcaggatat cgccctgctg cgtgcgaaag tgggcatggt gttccagaaa
     4561 ccgacgccgt tcccgatgtc catctacgac aacatcgcct ttggcgttcg tctgtttgag
     4621 aagctctccc gtgccgacat ggacgagcgc gtgcagtggg cattgaccaa agccgcattg
     4681 tggaacgaaa ccaaagataa attgcaccag agcggttact ctctctctgg tggtcagcaa
     4741 cagcgtctgt gtattgcgcg tggtatcgcc attcgcccgg aagtgctgct gctcgacgaa
     4801 ccgtgttcgg cgctcgaccc catctctacc gggcgtattg aagagctgat caccgaactg
     4861 aagcaggatt acaccgtggt gatcgtcacc cacaacatgc agcaggctgc gcgttgttcc
     4921 gaccacacgg catttatgta cctgggcgaa ttgattgagt tcagcaacac ggacgatctg
     4981 ttcaccaagc cagcgaagaa acaaacagaa gactacatca ccggtcgtta cggttgattc
     5041 aggagtgcgt tatggacagt ctcaatctta ataaacatat ttccggccag ttcaacgccg
     5101 aactggaaag tatccgcacg caggtgatga ccatgggcgg catggtggag cagcagcttt
     5161 ctgatgcaat caccgcgatg cataaccagg acagcgatct ggcgaagcgc gtgatcgaag
     5221 gcgacaagaa cgtcaacatg atggaagtgg cgatcgatga agcctgcgtg cgcattatcg
     5281 ccaaacgtca gccgacggcg agcgacctgc gactggttat ggtgatcagt aaaaccattg
     5341 ccgagctgga gcgtattggc gacgtggcgg acaaaatctg ccgtactgcg ctggagaaat
     5401 tctcccagca gcatcagccg ttgctggtaa gtctggagtc gctgggccgt cataccatcc
     5461 agatgctgca cgacgtgctg gacgcgttcg cgcggatgga cattgacgaa gcggtacgta
     5521 tttatcgtga agataaaaaa gtcgatcagg aatacgaagg tattgttcgt caactgatga
     5581 cctacatgat ggaagattcg cgcactattc cgagcgtact taccgcgctg ttctgtgcgc
     5641 gttcaatcga acgtatcggc gaccgctgcc agaatatttg tgagtttatc ttctactacg
     5701 tgaagggtca ggatttccgt catgtaggcg gtgatgagct ggataaactg ctggcgggaa
     5761 aagatagcga caaataattc accagacaaa tcccaat
//
LOCUS       DAZPAB010000216         5738 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZPAB010000216 DAZPAB010000000
VERSION     DAZPAB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.216"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..241
                     /locus_tag="ACHUPG_003600"
     CDS             <1..241
                     /locus_tag="ACHUPG_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863047.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="ACHUPG_003601"
     CDS             complement(238..1179)
                     /locus_tag="ACHUPG_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HFR0863048.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="ACHUPG_003602"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="ACHUPG_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="ACHUPG_003603"
     CDS             complement(1684..2904)
                     /locus_tag="ACHUPG_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR0863049.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="ACHUPG_003604"
     CDS             complement(2908..3651)
                     /locus_tag="ACHUPG_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR0863050.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="ACHUPG_003605"
     CDS             complement(3542..5008)
                     /locus_tag="ACHUPG_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HFR0863051.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5738)
                     /locus_tag="ACHUPG_003606"
                     /pseudo
     CDS             complement(5008..5738)
                     /locus_tag="ACHUPG_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
BASE COUNT         1226 a         1618 c         1435 g         1459 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatgat
//
LOCUS       DAZPAB010000217         5705 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZPAB010000217 DAZPAB010000000
VERSION     DAZPAB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.217"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1..411
                     /locus_tag="ACHUPG_003607"
                     /pseudo
     CDS             1..411
                     /locus_tag="ACHUPG_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="ACHUPG_003608"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="ACHUPG_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="ACHUPG_003609"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="ACHUPG_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HFR0863052.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1400 a         1430 c         1360 g         1515 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tagggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tggtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggggg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc gagaaccacg gcagcctgac ggctgcaaat tgaaaactgc
     5641 ccctgttcgt cctggtgttg ccgcacgctc cgcagaatat gtatgctctg aggacctgtt
     5701 ttatt
//
LOCUS       DAZPAB010000218         5702 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZPAB010000218 DAZPAB010000000
VERSION     DAZPAB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.218"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..170)
                     /locus_tag="ACHUPG_003610"
     CDS             complement(<1..170)
                     /locus_tag="ACHUPG_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863053.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="ACHUPG_003611"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="ACHUPG_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR0863054.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="ACHUPG_003612"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="ACHUPG_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /gene="ygbI"
                     /locus_tag="ACHUPG_003613"
     CDS             complement(3616..4383)
                     /gene="ygbI"
                     /locus_tag="ACHUPG_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR0863055.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5487
                     /gene="ltnD"
                     /locus_tag="ACHUPG_003614"
                     /pseudo
     CDS             4579..5487
                     /gene="ltnD"
                     /locus_tag="ACHUPG_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
     gene            5484..>5702
                     /locus_tag="ACHUPG_003615"
     CDS             5484..>5702
                     /locus_tag="ACHUPG_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HFR0863056.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
BASE COUNT         1410 a         1469 c         1481 g         1342 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
     5401 gccagtaacg cgggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
     5461 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
     5521 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
     5581 gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc
     5641 gctcctgtcc ggtggttgaa gccacacagc aatcgctggc ggctctgagc tggttgcaac
     5701 ag
//
LOCUS       DAZPAB010000219         5642 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZPAB010000219 DAZPAB010000000
VERSION     DAZPAB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.220"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..248)
                     /locus_tag="ACHUPG_003616"
     CDS             complement(<1..248)
                     /locus_tag="ACHUPG_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0863057.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
     gene            complement(335..696)
                     /locus_tag="ACHUPG_003617"
                     /pseudo
     CDS             complement(335..696)
                     /locus_tag="ACHUPG_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1035..1589)
                     /locus_tag="ACHUPG_003618"
                     /pseudo
     CDS             complement(1035..1589)
                     /locus_tag="ACHUPG_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1732..1854
                     /locus_tag="ACHUPG_003619"
                     /pseudo
     CDS             1732..1854
                     /locus_tag="ACHUPG_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2116..2682)
                     /gene="ipgB2"
                     /locus_tag="ACHUPG_003620"
     CDS             complement(2116..2682)
                     /gene="ipgB2"
                     /locus_tag="ACHUPG_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HFR0863058.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2964..3278)
                     /locus_tag="ACHUPG_003621"
     CDS             complement(2964..3278)
                     /locus_tag="ACHUPG_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863059.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3356..3466)
                     /locus_tag="ACHUPG_003622"
                     /pseudo
     CDS             complement(3356..3466)
                     /locus_tag="ACHUPG_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3790..4467)
                     /gene="ospD1"
                     /locus_tag="ACHUPG_003623"
     CDS             complement(3790..4467)
                     /gene="ospD1"
                     /locus_tag="ACHUPG_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HFR0863060.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4839..4976)
                     /locus_tag="ACHUPG_003624"
                     /pseudo
     CDS             complement(4839..4976)
                     /locus_tag="ACHUPG_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5058..5356)
                     /locus_tag="ACHUPG_003625"
                     /pseudo
     CDS             complement(5058..5356)
                     /locus_tag="ACHUPG_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1523 a         1229 c         1147 g         1743 t
ORIGIN      
        1 cgagcgatta ccgttgccgg gattttgtaa ggatgagcat cggcgatgcg ttgacgaata
       61 taatcccggt attcatccag gagtgaagca acagcaggtc gcggcgtata ttttggcggc
      121 tcagattttg cctgcaaata acgtttaacg gtattgcggg agagccccag ttctctggca
      181 atcgcccggc tactcattcc ctgcttgtgc aggattttaa tttccataac tgtctcaaaa
      241 gtgaccataa gctctcctga atcaggagag cagattaccc cctggatctg atttcaggcg
      301 ttgggtgtgg atcactattg caccgttcgt gacagtgttg ttgcgccttg ctgccaacga
      361 agctccactg cccatcaagt tcacagataa gggcaacatc agcgtgagcg actggcgcag
      421 atgttatccg ccggggcggg agctttttaa tgtccgtatg acggtattga tgccgatttt
      481 cagcgtacgg ccagtatcgc gaactccggc accgttgaaa gccatttcag tgatttggtt
      541 tttaactccc ggtttacggg cctcgtaaca gtaagtgagc tgaaacaccc ggtgacagtc
      601 acggcaacgg aatcgatcat gtccttttgg gttctgcccg tgacggtaaa cctgaacgga
      661 ctggcaacgg gggcaatgaa agttaactgt gaccatgaaa gaacctaaaa gcgggtatta
      721 taacccctaa ttcaactaat tggaggcatt gtcattttta atctatggat acacttaaaa
      781 atcctctcac cttacgttaa aaaccttctc atgtttagag ttttttatta tgtttatcat
      841 aatgatatga cgatctgagt ttttaaaatc aagaagaaaa gataagtttt ttaaatgcac
      901 agtgcctgtg gataactttg cgtaaatccg gaggacagat cactctgaag caagacaaca
      961 gcatctgaaa acaatatgct cacgtttcaa ccacagggcg taagtgatcc tacccacgta
     1021 atatggacac aggcctaagc gaggttctgg ttttcaaatt gttccggact gaggccgcca
     1081 caccaactgt gccgtcgcca gcgattgtaa tcacattcga tataattaaa cactgttgcc
     1141 ctcattattt cccggctgat aaagtgttct ccgtggagac attccacttt cagcgaatga
     1201 aagaagcttt ccacgcaggc attatcgtag cagcagccct ttgcgctcat actgccacgc
     1261 agattatgcc acttcagcag cgcctgataa tctgctgaac agtactgact tcctcggtcc
     1321 gagtgaacga taacgttccg gggtctctta cgccgccaca gcgccatctg cagggcatcg
     1381 caggccagtt gcgccgtcag gcgtggtgac atggaccagc caataacggc acgtgaccac
     1441 aggtcaatga ccacagccag atacagccag ccttcatctg tacgtaagta cgtgatgtct
     1501 cccgcccact tctggttcgg accactggcg taaaaatcct gctccagcag attttctgac
     1561 acaggcaggc catgtgcgcg gtaactgacc gtattgctgg gtaagatcaa tttgctcact
     1621 ggtatttact acgtgcatcg aatgagtgat caagagccgt atgacgcgag agtgtcacgt
     1681 acggttccgt aagcagccgg agagtgaaac tctctccggc tgactcgact cgcaggttcc
     1741 gacagcggtg acgaacatgc agcggtgttg ttctcgctga tcggcacatg ccgtctgaac
     1801 aatgtggagc cagaaaaatg gctgcgttac gttattgaac atatccagga ctgaccggca
     1861 aaccgggtac gcgatctgtt gccttggaaa gttgatctga cttctcagta aatatcaata
     1921 tgattctgac gagccgctta cctttgagca gagaaagggg agagggatcc cacatccatg
     1981 agctaggaaa atacaaaaac tggctataca acaattcaat atattaggac ttacttgcga
     2041 ggagaaatat agcaccgctc aggcaattcc tgggtattta cttttcttag tttctttact
     2101 cgggatgttc tgtcatcaga aaggcgattc taaatttgta atatagtcac tcatattccc
     2161 acgtaatttc tttagatcaa tatattgcac atttttctca aaaaactgat cggaaataag
     2221 atttgcaaca ttgcttgttg tatcattttt aacgcttcta ttcatccctt cacaaagttc
     2281 gtccattttc tttttgaaat aactgcttcc tgatacaaga tgatttatgg aactttgtgc
     2341 agcattgatg tctagagaaa tgcgataatc tttctcaacc tgtttaaata tctgtttacg
     2401 gagattatcg ccgatttttc ggctgtgttc tgcacaaaac ttatcaatac attggtttac
     2461 cacccgggat acgtagactt ttttttcatc atttttccaa tctgtaatat tttcaccgat
     2521 ttgttctgaa acagagctca aaatactagt atttattttg gttgatgata tttttactat
     2581 ccttttcccc attgtacagc ggataatctc atccaccttt cctgaaaaat atcttttgtg
     2641 agataaactg attccaaaat tattaaaaga tgttccaagc attataatca ccttgtcatg
     2701 taaaatagta acattgtcat tattttagct gattcaaaca attctgtaaa tgctttgagt
     2761 tctttactcc cttggctcaa tgatggaccg tctggaatcc cagagactaa actctctgag
     2821 aaagagagta tgaaaaaact tggggcagtc cctaaatgaa gggaccaaca gcggtaaaat
     2881 aaccggcatc attcataatg atgccctgtt agttgtagtt atgaagattt acgtcattaa
     2941 aattgctgtt cgtagtgtca gtcctatggt ctggcatcag ctgaggatcg tagccgatac
     3001 atcactggct tcacgacatt cgtgttgagg ctattcatga aaactccacg ctgaaaacgc
     3061 cgttttgtat aaacggtcac ggtatgcccg gagccactgt tgctgataaa ttcgataaaa
     3121 ccttggcgtt tcttgaagct atcgttaatg cggataatga aacaacgatt ggtgagattc
     3181 gctcttttgc cgacgccctg gacgctgtcc ggttcaatcg caacaaaatc aaccggcagc
     3241 tgagcaaacc caaccttgcc tctctggcgc tggagcatga agtgatttgg ctgggacgta
     3301 gccgctgaca gcttctataa aagtatgatc ctacccacgt aatatgaaca caggcttaag
     3361 tgaggttctg gttttcaaat tgttcaggac tgagacctcc acaccaactg tgccgtcgtc
     3421 agcgattgta atcgcacttg atataattaa acactgtata tttcatttgt ggagtctcca
     3481 gtctgagagg gctttatgca ttccgtattg ggcactatga tgccagaaat ctgcaaggct
     3541 ccacaccttc ctgtatttcc cttcctctat cacagtgcac tttacttaat agggtgggat
     3601 gtgttcatta catttattgt gttttttatg attgtttttg cgtccagtgt cgttcgtcat
     3661 gggaaactgg ttctatgggt agtatcgctc agtctagtta actgtttgtt tctttttaga
     3721 aattaagcag atcacacaat tcggggagag atccttctaa gtgcaattct ttgcagctat
     3781 ttagcacact taaaacaact cttgtatttt gtcagaaata atattaaaca tgtcttgttc
     3841 attattgtta agtctgtctg ctatttctgt aagttttatc ccactatgat ggcatatatt
     3901 tatatatgcc atcatagcct cgatattccc tttccctgcg gccagaaata atcctggtaa
     3961 cccctcatta tctcttacat ccaatagttt aatcgtttct tttggtgata aatcaaaagt
     4021 tttaataaaa tctccataag cttgaatcgc agcggaatga ccttcttgaa gagctatgaa
     4081 caaagcagat tccccagcat tatttcgtgc tgcaagaact tctagttttt cctctttggg
     4141 tatttcggct gccaacagcg attcaccgta tttacgcgct gcatcggcat ttccatgcca
     4201 aatcaatgaa aacaaattat ggcggttata ctccttatta ttctttgtcg gaatggatat
     4261 gttctgtatt ttggccaatt ttctgaaaga aacaccacta tatccccccc ctgatttttc
     4321 ttcattgatg gcgtgggata tagcggtatt tgtcacgtta atgatattat ttttcattcc
     4381 tttattttca ttagcagtat tgctgcctat tattaagtgc atatttttgt tgtttgctgg
     4441 atgtaatcca tagttattta ttgacatttt tgttcatcct ttttgctaaa aaaaaacaat
     4501 aacctatttt tgtaatgtaa tagatattag tctattgcta atctaaaaaa accattgcca
     4561 cattgaatgg ctatattcac caataaattc tttcgatttc gtatattgac atatcgtaga
     4621 tatatcttat ccctaagatt caataatcga gtccgtttac cattctgtgc aagcgccttc
     4681 actcagaagt gaccgtcaag gcgatcacca aacttgataa taaagcagct cattgccata
     4741 cgccagtcct taaggggcgt tgtccatttc tgtgatgctg cctagattta actgccagct
     4801 ccggcttatc tacatctaaa tatgtgagcc taataccgct acactttgtc cagactatgt
     4861 ttttctccgg gaagtgggct aaccgtctcc ataaagtgac gaactttttt caacagtggc
     4921 catattgaac ggcactgatg attacgggtt attgtctcat gaagagccgg ccacatcact
     4981 gacagcagtg acttcaggcc ttcagcacca gacagcctga accagttaat ccatcgcccg
     5041 acaaatttag taatctaggg ctggtttttc ttcagctgtt gcggttcgaa agtgccatta
     5101 cgatcacgca gagtacggat ttccagaggt tcatctccag tagtgataat cttcgttaag
     5161 caaccattgc gggaattgct gccagatcta agctgatttt tctcgcaccc aagatgatga
     5221 gtcatattag cattgagtac ggtttcattt tcagcatcca atcaaactga ctgagatctt
     5281 caggattttt gagacttttg gctagttcat tagctaaagc ccgcaactta ttagatgtca
     5341 actgtttttc attcataaat tcaccttcat ttgatatcaa attaaatagt tcaacatcag
     5401 agaattatac aaacttatgt acaggctcct atgatcttcg aatcctgaag attatagtga
     5461 aaataagttt tcacgttgaa atcccacata gttagtatat gataaataat catggatgac
     5521 ctgattaacc tcttggggta gtgcttttat agaagaataa atattctact aatttaatac
     5581 atcacctaaa atagtattgt tttttatggt tggttgaagg tcgtggtgat gatgttagtc
     5641 ct
//
LOCUS       DAZPAB010000220         5590 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZPAB010000220 DAZPAB010000000
VERSION     DAZPAB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.221"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(1..279)
                     /locus_tag="ACHUPG_003626"
     CDS             complement(1..279)
                     /locus_tag="ACHUPG_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863061.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="ACHUPG_003627"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="ACHUPG_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR0863062.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="ACHUPG_003628"
     CDS             complement(1254..1670)
                     /locus_tag="ACHUPG_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863063.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="ACHUPG_003629"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="ACHUPG_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2887
                     /locus_tag="ACHUPG_003630"
                     /pseudo
     CDS             1837..2887
                     /locus_tag="ACHUPG_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2915..3004)
                     /locus_tag="ACHUPG_003631"
     tRNA            complement(2915..3004)
                     /locus_tag="ACHUPG_003631"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="ACHUPG_003632"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="ACHUPG_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR0863064.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="ACHUPG_003633"
     tRNA            3996..4071
                     /locus_tag="ACHUPG_003633"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..5495
                     /locus_tag="ACHUPG_003634"
                     /pseudo
     CDS             4233..5495
                     /locus_tag="ACHUPG_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1508 a         1326 c         1293 g         1463 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     4861 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     4921 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     4981 agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg
     5041 tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac
     5101 agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc
     5161 ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg
     5221 tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca
     5281 gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc
     5341 aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca
     5401 aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc
     5461 cttatacaat tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg
     5521 caaattacca ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg
     5581 aaaaacacct
//
LOCUS       DAZPAB010000221         5586 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZPAB010000221 DAZPAB010000000
VERSION     DAZPAB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.222"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..92)
                     /locus_tag="ACHUPG_003635"
     CDS             complement(<1..92)
                     /locus_tag="ACHUPG_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR0863065.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCT"
     gene            complement(102..877)
                     /locus_tag="ACHUPG_003636"
                     /pseudo
     CDS             complement(102..877)
                     /locus_tag="ACHUPG_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
     gene            complement(895..1947)
                     /locus_tag="ACHUPG_003637"
     CDS             complement(895..1947)
                     /locus_tag="ACHUPG_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR0863066.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(1978..2820)
                     /locus_tag="ACHUPG_003638"
     CDS             complement(1978..2820)
                     /locus_tag="ACHUPG_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR0863067.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(2807..3688)
                     /locus_tag="ACHUPG_003639"
     CDS             complement(2807..3688)
                     /locus_tag="ACHUPG_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR0863068.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(3709..4992)
                     /locus_tag="ACHUPG_003640"
                     /pseudo
     CDS             complement(3709..4992)
                     /locus_tag="ACHUPG_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(5006..>5586)
                     /gene="ycjM"
                     /locus_tag="ACHUPG_003641"
     CDS             complement(5006..>5586)
                     /gene="ycjM"
                     /locus_tag="ACHUPG_003641"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFR0863069.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         1365 a         1508 c         1395 g         1318 t
ORIGIN      
        1 gtgcagtagt acgaagcatg aaccttatcc gccacctggc ccgcgcctat tatcgcgacc
       61 cgcagcggag agcttgtcat tgcacttttc acaaagacct cttagcaggt acgcaaccag
      121 gccaacgaat cacggtacgc ctgggcagga tcttctgccc ggatacgccc ttcatacacc
      181 acataaccct gatagttatc ggcgcgcagc tgttcaaaca gcgcgtggaa atcgagggtg
      241 ccactgcccg gctggtaacg atggttatcg gcaatatgca catgaccgag caagtcacgg
      301 ttgtcgtgca gcgcctgcgc caggttatct tcttcgatgt tcatgtgata gaaatcgccg
      361 ataatctgca catgtttaag attgttttcg acgatgtaac gacgagcatc ggcgagggtg
      421 ttgatcatat gatcctgata gcggtttaac ggttcgagat acaccacggt tccggtacgc
      481 gcggcgacct gttccagtac gcgcagggaa tcactcacca gtttgcggtc gccgtccagg
      541 ctacgcggcg aggtcatcgg cggtaagcgg aaggtaaaca tgccccacgc tgccggaacc
      601 cccccacttc tgccagcgcc tcgagaatgc gttcgatctg ctttaagcca ttaagacgac
      661 gctcttcgat aaagtcacca atccagccgt catagccgcc gcaggcggtg gtcactggta
      721 aaccggtttc tttgatcgcc gctttgactt cttcgatgtt gttgaccagc agtttgccgt
      781 caatctcaaa accatcgaag cccatctctt tgatataacg aaatctctcg cggatatttt
      841 ccggaaagaa tgcctgattc tgtgtgccga ttttcatttt ttcattcctt tgaattaaaa
      901 cgtaacgccc attttgatgc tctgttccgg atgctgatcg acatactgca tgtagctttc
      961 cgggctgttg gcaaaggtca ctaccgggtc gattaaatct tcgcaattga gataaccgtt
     1021 catcagcagt tcccagcagg tttcttcaat acgcttacgg ctccagcgcg gataatccgg
     1081 gttcggttcg ctgcacgcgc gggagaaaac aattttggcg ttattgaaat gcgcttcgcg
     1141 tccgaggtta aaaccttcag caaatggttt ggcaaacgcg acataggaga tggtgccgcc
     1201 gtaggccagg ccgcgcagcg ccgattgcag cgcgtcggcg taaccgctgg tttcgataat
     1261 cacatcagcc ccctgcttgc cggtcagcgt tttgatctct ttgcctacgt cagtgccaat
     1321 ggggttaagg cagaaatcag caccgtggcg acgggcaata tcacagcgat gggcgatagg
     1381 atcgacgcca attaccaccg atgcgccagc gcgtttcgcc agttggatgg caatttgacc
     1441 gatcgcgcca agccctacca ccacgacaaa atcccctacg cgtacgttgg catcgcgcac
     1501 gccgctcatg gcaaactgcg ccgggtcgta acagacggcg tttttccagg agctgccttg
     1561 cggcattttg cgcagcttgt agttattcac tgcgttaatg atgaccgtct cggagagcgg
     1621 gccgtagccg catacgctgt cgcccaccgc gtagtctgta acgtcgctgc cgcactcgat
     1681 aatgtcgcca accaccatgt tgccaagctg gaatttgcca aactcaatgc cgcgcggcgc
     1741 atctgccgga cgcggggtga acatctgcca ctcgccgtta aagtcttcat cgataaacgg
     1801 gctggcggcg cggaagtcga ccacttccgt tccgtgtttc ggtgcgccga aacgcacgcg
     1861 gatcttcact tcattggcta aaatcgcccg atcttcatac tcaaccagcg cagcaacacg
     1921 cggtgctgtg gctactaact ttttcatgta aaactccttg taaagaaact ggccgcttta
     1981 gcccttcacg ccaccggcgg tcaaaccact cttaataaaa cgttccaaaa gcgcatacat
     2041 aatcaccacc ggcaatgcgg tcaccagcga ggccgccatc atccgtcccc agatgtaatc
     2101 aggcgtactg aacagcgcgt tcaggcccac tggtaaggtg aaattgctgg cgctggagag
     2161 gaaaatcgac gcaaacagat agtcgttcca cgccaccata aagcagtaga cgaacaccga
     2221 tatcagcccg gacatcgcca ggggcacggt gatacggaag atgatttgca gacggttgag
     2281 gccatccatc atcgccgctt cttcgatctc atcagggatg gtgtcgaagt aacttttcag
     2341 cataaacacg gcggtgggca gggtttgcgt caccatggta atgatcagcg ccatctcggt
     2401 gtcgtaaata ccaagcgcgg tgatgatttt gaacagaggc accaccagca aaatgccaga
     2461 gaacatatac accgtgtaaa agctggcgtt gatggtcatc cggcctttaa agcgcaggcg
     2521 ggaaagcgcg taagctccga gaatgccgag aaataccgcc accacggagg aaaccaccga
     2581 caccaccatg ctgttacgga agtagtcgac aaacgggaaa atcatcgggt taaaaatgtc
     2641 gacgtaatgc tccagcgtcc actgttgcgg cagcagcgta ggatgcagcg atatcgcctc
     2701 tttcgcgctt ttgaacgagg tcatcagcat cacaaaaaac ggaaacagcg tgatgatgag
     2761 aaacagcgcc agcccgcagt aaaaaccgat acgactgagc gtgcgtttat ttgttgccat
     2821 tgaggttcac ccgtttcctg gtcagcagaa tgacagcgaa aataatgatg aagagcacca
     2881 ccgagatcgc cgccgctttc cctaagtcat taaaggcaaa ggcggttttg tagagataga
     2941 caccaagaat atcgaccttg gttgtcagca gataaacatc agcgaacatg tagaacatcc
     3001 agatggtgcg cagggtcacc accgtcgcca ggaccggcat aattgcgggc agcgtgacga
     3061 tgcgaaaccg ctgccaggca ttagcgccat ccatctctgc cgcttcgtac agcgatttgt
     3121 cgatggtctg caaaatcgcc agaaacgaga taaaggcata cgggaagtag cgccagatgg
     3181 cgaacagcac caccagcaca aagctactgc ccggattatc aaaccacagc ggtgcctgct
     3241 catagagatg cagaagatcg acgccgaggt agttcacaat gccgtagccg ttgttgaaca
     3301 tgtatttcca ggcaaacacc agcgaaatgg acggcgttac gtaggagagg atcaccagcg
     3361 aacgcgcggt tttgcgcagg cggaactcgc ggttgaaaaa catcgccacg gccagcccga
     3421 gcacggtgct gcccgccacc accagcgccg tataccagac cgtcatccac agcgagtgcc
     3481 agaagccggg atcggagaga atgcgcacat agttactcac cccaacaaac gttgactcga
     3541 tgttgggatt gagcggcaga cgtaaaaaac tgatttcgat attcgacacc atcggccacg
     3601 ccaccagacc gcccagcagt aataagctgg gcgcgagaag cagcagcgca aacggcatat
     3661 cggaacgacc tgaaaaaagc ctgttcattg cggattcctg tcctgggttt agtgctgttc
     3721 gatcagttca tccagctttt tctggctcgc ttgcagcgtg ccagggagat cggctttacc
     3781 cacggtgacg ttatgcacca tggagctcac cacaccagaa cccgtcacat cgcccatgcg
     3841 ggtaaagttt ttatccccta ctgcgccaaa gacctgaata tttggcagtt caccgattag
     3901 ctgattcggt agttcaccca gcgccttaat aacgtcgttg tctttccagg tggcggtagt
     3961 gacaaccgct ttattcaccg gcagcgcagc acctggcgac atcatcaccc agtcggcaat
     4021 gttgtccgcc tgctccataa aggtgacaaa tttttctgct gcttcggtct tttgtccggc
     4081 ggtaatggtc agcgaggtca acatgccgta gaccgcagag tttttctcgg ttggcaccac
     4141 gaaaccgacg tttttcgggt cgccttcttt aatcacagcc ggaaggatat aggtggagta
     4201 aatcgccatc ggcgcggtgc cgttcataaa ggcgtctttc acttccatga tgtcgttaga
     4261 acccggcatg gtgttggcag caaggtcgcg gtaataggtc agcgcctgca tcatctctgg
     4321 tgtatcaagg gtgattttgc cttcggcgtt aaagacgttg ccctagttgg ataacgcaaa
     4381 ctgggagaag gattgttccg tcagcacgct ttctgccgta ggcagcgcaa taccgtattt
     4441 tttattcgcc gggtcattca gtttctgtgc aacgtccagc agctgttgcc agtttttcgg
     4501 ctcctccagc cccgcttttg ccagcacatc tttgcgatac cagatgccgc caatccaggc
     4561 gctgacagga acaccggtcc atgcgctacc atcttcggta cgcacaatac gcagtacgcc
     4621 atcgtaaaac gcgccttcac caacgttgct gatgaccgtg gcaaccgctt tgcgatcgat
     4681 aagctgctct ttatccataa ctttggcgta gtcatggctg gtttcgatca cttccggcag
     4741 cgagccgcta cgtgaaaggg tgatgacttt ggtgttatga gcgtcttctt ccacgggcac
     4801 ttgtttaacg gtgatacctg ggttttcttt ttcaaaacgg gcaatcaatt tgctgataac
     4861 tgcctgacgc tcctgctcca ccgaagaatg cataaattcg atggatacat gggttttatt
     4921 ttcttcttta cagcctgaaa tcagggcgca ggaaaccagt gctgataaca gcacgatttt
     4981 tgatttaatc atgagatgtt cctttttatt taatccacat aacctgccag gggcgcagcg
     5041 ttatttcatt acccaatata tcaacttcac taatcagatc ccgaccatgc agatcagtaa
     5101 tatttacatg ctgaatattt ttactgacat taaacaatcc agtcagacaa ttaccatcag
     5161 cgttacttct ttgaatacgc attacggatg aattaaccgt atcgatggta aaattattat
     5221 ccggatgaaa ctcgttatgg ctgcgacgaa gtgtaattaa acgcgacaac tcatgatata
     5281 ccgcatgcct taatgtagct tcatcgttca gttctcgtgt tatctcttca ctgtgatatt
     5341 ttttacggtt aatcgcacgg ttatatccga gtttttcgac acctgcgtaa tcattacgcg
     5401 agccaagaat actttgaata tatatcgcgg gtacaccggg gaaacttaac aaaatcgcat
     5461 gggcaaggat aaacctggtg caacgttctt catcgctact ctcgcggcgg cttaacgcat
     5521 tcatataggt gacattcatc tcatagggac tgcgcgtgcc gtcgggatta tttttccagt
     5581 ttacta
//
LOCUS       DAZPAB010000222         5583 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZPAB010000222 DAZPAB010000000
VERSION     DAZPAB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.223"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..857
                     /locus_tag="ACHUPG_003642"
     CDS             <1..857
                     /locus_tag="ACHUPG_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFR0863070.1"
                     /translation="ELTRVGVDPNNVAQMYQQNPSGFGEFVDHLGMAALGPIDYFNVQ
                     DKMAGREIDRGRLAETIRSNQAGEALTARGQDIQIRGQNISAQNAALSREIQRAELQE
                     KALDRQIARESNQLKLEELKQKQADVRQKAEIARADRQAAAQGAVDTFSTALDSLNEI
                     EQSPGLSKAVGIRSAFPTVPGSDAANFEARLDTFKAQTFLPMVQSLKGMGALSDAEGK
                     KLSDAVGALSPKMSEKAFRDSIGKIRNQLESKLSTVKKQFDYQEPVQNTPGQQSPAGS
                     NFSSLWGD"
     gene            857..2854
                     /locus_tag="ACHUPG_003643"
     CDS             857..2854
                     /locus_tag="ACHUPG_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR0863071.1"
                     /translation="MAKAWKDVIASPQYQALAPEQKAQAQEQYFNEVVAPQAGENAEQ
                     AKQAFYAAYPLPSVQPVETQQPVAQQQPQQSGFMSDLGEAVKETGRGLVQAGVNVANI
                     PASVADAVTSAAAWAGGKLGIGDGTYQPAPRVTTHGLEQDFGLQQGALTPQTTEGRVF
                     AEALPYLTPAGVERAATQAPTLAGRIAQGATRLLAENAVGSLAANSAKDDAEALATDL
                     GVGVLAGGAINAAGRGLGAAYRGVRGAIAPEAQQAIRFAEREGVPLHTTDLLQPTSRV
                     GKMAQTTAENIPLAGTSGMRATQQEARSQLVQRFADKFGEYDPAVVIDSLKAKTSGIR
                     RAAGNRLEQVQNAMAGVNIQPARAIQQIDTEISNLQKLGKVADNETISKLQSYRDELV
                     RNAGPEGPVNLDLKQLSDLRSQFRMDVKGERPVLPNRSDAAIQRVYKAMTDDINGAIG
                     QNLGNDTLRKYQQANAVYADEAAKLKNTRLKNVLMKGDLTPEVVNNMLFSKNKSEIKT
                     LYNSVGRVGRVQMRNGIIGKAMEKSGGSPDQFLRQLNILQNQTGITFKGQDAAYLKGL
                     KNYLQSTQQAAKAAVTTPTGQQTIPFIIGYGTAMNPATTGAAVSYGLLTRAYESEPFR
                     NAMLRMANTPRGSTAFEKAMQQAQKAINALTQGAKSDALSE"
     gene            complement(2944..3204)
                     /locus_tag="ACHUPG_003644"
     CDS             complement(2944..3204)
                     /locus_tag="ACHUPG_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFR0863072.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQRAKMNNRSLNSEIIAAIEES
                     LDKQNSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSS"
     gene            complement(3336..3794)
                     /locus_tag="ACHUPG_003645"
     CDS             complement(3336..3794)
                     /locus_tag="ACHUPG_003645"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR0863073.1"
                     /translation="MEVLYYNKETGIFTWKKKLSARGAVGKVAGTISYGYNAINIDGV
                     RYFAHRLAWLYVYGEWPKQEIDHIDRNRRNNAISNLRDVSRVVNALNVGPRNSNAGIK
                     GVTFCQARNQWQAQINVSGKNITLGRFNTIDEAAIAYKAANMVADHLLSR"
     gene            3859..5571
                     /locus_tag="ACHUPG_003646"
     CDS             3859..5571
                     /locus_tag="ACHUPG_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-binding domain-containing protein"
                     /protein_id="HFR0863074.1"
                     /translation="MPEQLYNVVVSQPSQLFTLARSFKANANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHVPVSQPIVINAAGYPVYNGQIAKFVTEQGHSMAVYDAYGSQQFY
                     FPNLLKYDPDQLKQLLQSDEGATYIGTNSGKNLQDELFAIKNEIYQDPLTSWPFGGTL
                     KIKQGVYDVTTPLVLNYDDYPADFVGAPGVRCFYSGENMAETILECRATDFNVKMLGD
                     AAFNSQRLAAYDYIGNMTLKGNPGSYGMLVQNKAHTRLENIVICRHPDGEGLRTDSML
                     TSDMTNVYLQSNRIGWRATNSVNHSELNAITANRLTCSQNSEWGILGDRWGAGTTINS
                     LTCEGNGTQGDSSTGGAQLAINGLNGSCALVLNNPYFEANAGGADLAIDNTGTRPVTV
                     VINGGNFHRVSSARYTHTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSADRPYWITGA
                     NCEVVDIGCVFTETTSKATSASALSVTRSGKINANGSIDVATGVSSVNVVATGVYEVS
                     FSHPLAAVASGYIVQITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSI
                     TRLL"
BASE COUNT         1547 a         1257 c         1368 g         1411 t
ORIGIN      
        1 aggaactgac tcgcgtcggc gttgatccta acaacgttgc tcagatgtat cagcagaatc
       61 cttcaggatt tggtgagttt gttgatcacc ttgggatggc tgctcttggt ccgattgatt
      121 acttcaatgt tcaggacaag atggctggtc gtgagattga ccgaggtagg ctggcagaga
      181 caatccgcag caatcaggct ggcgaggcac taacagcacg aggtcaggac atccagatac
      241 gtggccagaa catcagcgca cagaatgctg ctctttcccg cgaaatacaa agagcagaat
      301 tacaagaaaa ggctctggac agacagatag ccagagaaag caatcagtta aagcttgaag
      361 agctaaagca gaaacaggca gatgttcggc aaaaggctga aatagctcgt gctgacagac
      421 aggccgccgc tcagggcgct gttgatacgt tcagcactgc gcttgattct ctcaacgaga
      481 tagagcaaag ccctggactt tcaaaagcag ttggtattcg ctcagcgttt ccgacagttc
      541 ctggatctga tgcggctaac tttgaagcaa gactcgacac ctttaaagct caaactttcc
      601 tccctatggt gcagtccctg aaggggatgg gggctctttc agatgctgag ggtaaaaaat
      661 tatccgatgc ggttggtgct ctaagtccca aaatgagtga aaaggctttt cgtgactcta
      721 tcggaaagat tcgaaatcag cttgaaagca agttgagcac tgttaaaaaa cagtttgatt
      781 accaggagcc ggtgcagaat acgccagggc aacaatctcc tgctggcagt aatttttctt
      841 cactatgggg tgattaatgg ctaaagcatg gaaagatgtt atcgcatctc cacagtatca
      901 ggcgttagca ccagaacaaa aagcgcaggc tcaggagcaa tacttcaatg aagtcgttgc
      961 cccgcaagcc ggagaaaatg cagagcaggc taagcaagct ttctatgctg cctatccatt
     1021 gccatctgtg cagccagtgg agacacagca accagtagca cagcaacaac cacagcaaag
     1081 tggatttatg tctgatcttg gcgaagcagt aaaagagact ggtcgcggac tggtgcaggc
     1141 tggcgtgaac gtggcaaaca tacctgcatc ggttgccgat gctgtaacaa gcgcggctgc
     1201 atgggctggc ggtaaactcg gcattggcga tggaacatat caaccagcgc cacgagtaac
     1261 aacgcatgga ttagagcagg actttggcct tcagcaaggt gcgctgactc cacaaacgac
     1321 agagggcagg gtatttgctg aagcattgcc ttacctcact cctgccggcg ttgagagagc
     1381 ggcaacacag gcaccaacac ttgctggtcg aattgctcag ggtgcaactc gccttctcgc
     1441 tgaaaacgca gttggatcac ttgctgcaaa tagtgcgaaa gatgatgcgg aagcactcgc
     1501 caccgattta ggcgttggtg tacttgctgg cggtgctatt aacgctgccg gacgtggatt
     1561 aggtgctgct tatcgtggcg ttcgtggtgc tatcgcgcca gaagctcagc aggctatcag
     1621 atttgcagag cgtgaaggag ttcctctgca caccacagac ctgttacaac caacttcccg
     1681 cgtcggaaaa atggcgcaga ctacagcaga aaatatccct ctggctggca caagcggaat
     1741 gagagcaacg caacaggaag cgagaagcca gttggtgcag agatttgctg ataaattcgg
     1801 ggagtatgat ccagcggttg ttattgacag ccttaaagcg aaaacatcag gaattcgtcg
     1861 tgccgccggt aatcgactgg agcaggttca gaatgctatg gctggagtaa acattcagcc
     1921 tgcgcgagca attcagcaga ttgatacaga aatatctaac ctgcagaagc ttggtaaggt
     1981 ggctgataac gagacaattt caaaacttca gtcatatcgt gatgagcttg ttcgcaatgc
     2041 tggccctgaa ggtccggtaa atctggattt gaagcaatta agcgacctgc gcagtcagtt
     2101 cagaatggac gtgaagggtg agcgaccagt gttaccaaac cgttccgatg ctgccattca
     2161 gcgcgtttac aaggcgatga ccgacgatat caatggtgcc attggtcaga atcttggcaa
     2221 cgatactctc cgtaaatatc agcaggccaa tgccgtctac gctgacgaag cggcgaaact
     2281 aaagaatacc aggctgaaga atgttctcat gaaaggcgac ctgacgccgg aagttgtcaa
     2341 caacatgcta ttcagcaaga acaaatcgga aattaagacg ctgtataact cagttggtcg
     2401 tgttggcagg gtgcaaatgc gcaatggcat cattggaaag gcgatggaga aatctggcgg
     2461 atcccctgac cagttccttc ggcagcttaa catcctgcaa aaccagactg gcatcacatt
     2521 taaggggcag gacgctgctt atctgaaagg attgaaaaat taccttcagt ccactcagca
     2581 ggcagcaaaa gcggcagtaa caacaccaac agggcagcaa accatcccgt tcattatcgg
     2641 gtatgggacg gcaatgaacc cggcgacaac tggcgcagcg gtaagctacg gacttcttac
     2701 tcgcgcctat gagagcgagc cattcagaaa tgcaatgctc cgaatggcaa ataccccacg
     2761 cggatcaaca gcgtttgaga aagccatgca gcaggcgcaa aaggccatta acgccctgac
     2821 tcagggtgcc aagtctgatg cgttgtcaga atagcttcgc aaacaccagg aacgtgcaaa
     2881 aaccaaatat gtagaacgca atattcagca tatctctttg cataaatcct ccgtaactga
     2941 tggttagctg ctgtcttttt tatatagctc tttgagcgta tcaaagacaa ttttcttaac
     3001 catatcggat tgttgttctg ccatacgctc tgcatcgtca atgtaaactg atgcagagtt
     3061 ttgtttatcc agtgattctt caatcgctgc aattatctct gagttcagag acctgttatt
     3121 catcttcgca cgctgcttaa ttttcgcgtg gagttcatgc ggaagtctca agtgaaactg
     3181 cgcctcgtcg tatttgctgt acatccttga tgcctcacca attgggtgga atggcatcgt
     3241 aacttactgg ataaatactc aatagtacca tttcggtatg caatcacatc atggttgcat
     3301 catatcattc gtctggagca atgaaatgtc agatatcacc tactcaacag atggtcagca
     3361 accatgtttg ctgccttata agctatagcc gcttcatcaa tggtgttgaa tctcccaagg
     3421 gttatgtttt tacctgagac atttatctga gcttgccatt gatttctggc ttgacagaaa
     3481 gttaccccct tgattccagc attgctattt cgaggtccga catttaatgc atttacgact
     3541 ctgctgacat ccctaaggtt tgagattgcg ttatttcttc tgtttctatc aatgtggtca
     3601 atctcttgtt tgggccattc gccatataca tacagccagg caagtctatg tgcaaaatat
     3661 cttacgccat caatattaat tgcgttatat ccataagaaa ttgtgccagc aactttccca
     3721 acagcacctc tggcgcttaa tttctttttc caagtgaaaa ttcctgtttc tttattgtaa
     3781 tagagaacct ccataaggcg ctcccgagtt actacctcgt gacgtctctt actcattatt
     3841 ttctccggaa tgtttattat gccagaacaa ttatacaacg ttgttgtttc acaaccaagt
     3901 cagttattta ctttagctcg ctcgtttaaa gcaaatgcca atggaaaaat ttatatcggg
     3961 aaaattgaca ctgacccggt aaatcctgaa aaccagattc aggtttatgt agagaatgaa
     4021 gacggatctc acgtccctgt ttctcagcca attgttatca atgctgccgg atatcctgtg
     4081 tacaacgggc agattgccaa atttgtaact gagcaaggcc attcaatggc tgtatatgat
     4141 gcgtatggtt cgcaacagtt ctattttccg aatttgctga agtatgaccc ggatcagtta
     4201 aagcagttac ttcaatctga tgaaggggct acttacattg gcactaatag cgggaaaaat
     4261 cttcaagatg agctatttgc aataaaaaat gaaatatatc aagacccgct tacatcgtgg
     4321 cctttcggtg gaacattaaa aataaagcaa ggggtgtatg atgttaccac tcctctggta
     4381 cttaattatg atgattaccc tgccgacttt gtaggtgctc cgggtgtccg ctgtttttac
     4441 tcaggcgaga acatggctga gacaatactg gaatgtagag ctactgattt taacgtaaaa
     4501 atgctaggtg acgcagcgtt taacagtcag agattggctg catatgacta catcggcaac
     4561 atgacgctga aagggaatcc cggaagttac ggtatgctgg tgcagaataa agcacatact
     4621 cgtcttgaaa acattgttat ctgccgccac cctgacgggg aagggttacg cactgactcg
     4681 atgctcacga gcgacatgac gaacgtgtac ttgcagagta atcgtatcgg ttggcgtgct
     4741 accaactccg taaatcattc tgaactgaac gctataactg caaataggct tacgtgcagt
     4801 cagaacagtg aatggggtat cctcggggac cgctggggtg ctggtacaac tatcaatagc
     4861 ctgacttgtg aaggaaatgg tactcagggg gattccagta caggtggtgc gcaattagcc
     4921 ataaatggcc ttaacggttc gtgcgctttg gtattaaaca acccatactt tgaggctaat
     4981 gccggggggg cagaccttgc tatagataat actgggacgc gccctgtaac tgtggttata
     5041 aacggtggaa attttcatcg cgtgagcagt gcaagataca cgcacactaa tattcaagta
     5101 acaagctcag gtgggggaaa agttacagtc atactcaacg gcacaacgtt ccagagcgca
     5161 ggggattatc agccaagtgc agatcgacca tactggataa caggagctaa ttgcgaggta
     5221 gttgacattg ggtgtgtttt tacagaaaca accagtaagg caacatcggc ttctgcattg
     5281 tcagtcacac gctcgggaaa aattaacgcc aatggtagta tcgacgttgc tactggtgtt
     5341 tcatcggtta acgtggttgc tacaggtgtg tatgaggtta gcttttctca tccgctcgca
     5401 gcagttgcta gcggttatat tgtgcagata acacctatat ctgcgcctga ttcagttagt
     5461 tgtgatgtta cttatattgg cgtagataca tttagggtga ccctgcgcaa tacgcttagc
     5521 ggagcaggaa tctctagttc gtttgcattt agcattacca ggctactata aacacgacag
     5581 cct
//
LOCUS       DAZPAB010000223         5557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZPAB010000223 DAZPAB010000000
VERSION     DAZPAB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.224"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..641
                     /locus_tag="ACHUPG_003647"
     CDS             <1..641
                     /locus_tag="ACHUPG_003647"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HFR0863075.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCAL
                     GLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(695..1300)
                     /gene="azoR"
                     /locus_tag="ACHUPG_003648"
     CDS             complement(695..1300)
                     /gene="azoR"
                     /locus_tag="ACHUPG_003648"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR0863076.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1501..5403
                     /gene="hrpA"
                     /locus_tag="ACHUPG_003649"
     CDS             1501..5403
                     /gene="hrpA"
                     /locus_tag="ACHUPG_003649"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR0863077.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQARRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLRYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1377 a         1358 c         1540 g         1282 t
ORIGIN      
        1 tcgtaggcgc gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag
       61 tctggttatg ttggccagta ttatcgctac tcattattgg ccgtgggtgc ggtgggcttg
      121 gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga
      181 cattgccctg gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc
      241 cggtgagcaa aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac
      301 agaatgcggt tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac
      361 tctatttctg tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg
      421 ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat
      481 tgggattatc gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta
      541 aaacagttga tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg
      601 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
      661 gaatatctac agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg
      721 ctgcttttgc gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg
      781 cgaagacaaa tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg
      841 tcaccaggtc cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt
      901 ttttacccgt taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc
      961 gtgcaaccag gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg
     1021 ccgcaataac gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca
     1081 gagcttcctg ctgacgcgga gtcagcggcg catcgctcgg acgcagagcg ccaaccagtt
     1141 cgccatccag taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg
     1201 agtgcttttc gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc
     1261 ctgccaggat gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt
     1321 tgaatggacg atgccccgtt gcttgctgac actttattca caatcctgcc acagagatcg
     1381 cgcaataaat cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg
     1441 gtactctata tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt
     1501 atgacagaac aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg
     1561 ctgcgcgaca gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct
     1621 gatgcacaac aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa
     1681 gtcctgctgc gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt
     1741 cagaaaaaac aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg
     1801 gaaaccggct ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt
     1861 attaaagggc tgatcggtca tacccaggcg cgtcgcctgg cggcacgaac ggtggccaac
     1921 cgtattgcgg aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc
     1981 agcgatcacg taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg
     2041 gagatccagc aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac
     2101 gaacgcagcc tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt
     2161 cctgacctaa aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac
     2221 tttaataatg cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat
     2281 cgcccgattg ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac
     2341 gccgtagacg aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag
     2401 cgggaaattc gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc
     2461 ttgccgcttt atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc
     2521 ggacggcgca ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt
     2581 aaatacgtca tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg
     2641 cagcgtttgc cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt
     2701 ggtcgtgtgt ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc
     2761 ccggagttta ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg
     2821 accgcgctgg ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc
     2881 aatatccagg atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag
     2941 gccagcgcct ataaactgac gccgctcggt cgccagctct cgcagttgcc tgtcgaccca
     3001 cgtctggcgc gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt
     3061 atcacgtccg cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca
     3121 tcggacgaaa aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat
     3181 ctgtggaatt atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg
     3241 tgtcgtaccg attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag
     3301 ttgcgccagg tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt
     3361 gaaattcaca tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat
     3421 aaacaggaat ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta
     3481 ttcaaaaagc cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg
     3541 gggcgcattg ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt
     3601 aaacgcacct acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa
     3661 aaagtcactg tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagatc
     3721 gattcggcgt tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag
     3781 acgcgtcacg cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa
     3841 cacaaatcac gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac
     3901 cagcgcatca gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc
     3961 agccgcgaaa cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg
     4021 gaaaaaatca gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt
     4081 ttgagatatc agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg
     4141 ccgttactta accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc
     4201 gaattggtga ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct
     4261 gcaccaaact acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc
     4321 gacagccttg agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg
     4381 cactgggatc aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac
     4441 aagaagctaa aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg
     4501 caggaaacgc tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg
     4561 agttttggtc agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg
     4621 tggccggcgc tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg
     4681 gagcaaaaac aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg
     4741 ccgatcaagt atttacatga gaagttacca aacaaagcca agctggggct gtactttaac
     4801 ccgtatggca aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg
     4861 atcgacgcca atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa
     4921 gtgcgcgccg aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc
     4981 actgcggtgt tcaatatcaa caaacgtctg aaagggcggg tggatatgac catggcactg
     5041 gggctttctg atattaaagc gcagatgggc gggttggtat atcgcggttt tgtcactggt
     5101 aacggcttca aacggctggg cgacacgctg cgatatttgc aggcgattga aaaacgactg
     5161 gaaaaactgg cggttgatcc acatcgcgat cgtgcacaga tgctgaaagt cgaaaacgtc
     5221 cagcaggcgt ggcagcaatg gatcaacaaa ctgccgcctg cacgtcgtga ggatgaagac
     5281 gtgaaagaga tccgttggat gatagaagag ctgcgcgtta gttacttcgc tcaacaactt
     5341 ggtacgcctt atccgatttc agataagcgt attttgcagg cgatggagca gattagcggt
     5401 taaccctgct atttgcctaa taaatctaaa acccggtaag catttagcgc cgggtttttt
     5461 attaattcta aaacggtaag gacaaaaatt caggaattca gaaaaataca attctcagct
     5521 gcaagatgaa taatgtttat ctacagcatt tccttaa
//
LOCUS       DAZPAB010000224         5485 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZPAB010000224 DAZPAB010000000
VERSION     DAZPAB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.225"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..1630)
                     /locus_tag="ACHUPG_003650"
     CDS             complement(<1..1630)
                     /locus_tag="ACHUPG_003650"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR0863078.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPW"
     gene            complement(1646..2541)
                     /locus_tag="ACHUPG_003651"
                     /pseudo
     CDS             complement(1646..2541)
                     /locus_tag="ACHUPG_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
     gene            complement(2541..3146)
                     /locus_tag="ACHUPG_003652"
     CDS             complement(2541..3146)
                     /locus_tag="ACHUPG_003652"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR0863079.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3317..>5485)
                     /locus_tag="ACHUPG_003653"
     CDS             complement(3317..>5485)
                     /locus_tag="ACHUPG_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFR0863080.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1581 a         1364 c         1141 g         1399 t
ORIGIN      
        1 cccacggttt accatcttta tgactggtta acaccccggc aatcagctcc agttgtgggt
       61 gccacggctg cccggtgtag atgagaatgc cacccggttc gatggcattg gctagacctg
      121 cgagtgagtt ttttacctgc tcattttcgg gaaaaagctc atataggcca gagacaatcg
      181 ccagcgtagg ccgcggagtt aacgcgctga gttcctccgg gttaaacgca tcgccctgtt
      241 caaaacgcac ccgcccagac attccccgtt gggcaatcat ctcttgcccc tgtgcaacat
      301 ttaactcact gtaatcacgt aacaaaatat cgcttacggc aggctcgttt gccagcgcat
      361 ccagtacata gcgtccatgc cctgcggcaa tgtcaaccac gcggacggct aaacctttgg
      421 cgtggagatc ggcaacggct tgtttaatca atatttgcaa atgggttttg cgctggcgaa
      481 taccgcgcca tccaacactg ttcaggtaga ttttgtcgat taaacgcccg aatgcgttac
      541 tgccttgcgg ttgattttga tagacataat ccagggaact gcctgaatca aagccggtac
      601 tcattccgag atgcaggccc gcagagtgcg caccgaacaa tttcatcgcc tttcgcataa
      661 agcgatacgc taaatcaacc ggcgataatg gcacgggtcc accagaaagg agccgccatc
      721 gatccgctga tggtcctgtg cggtcttcat gctgataatc aaatttttgc gatttgttag
      781 catataagcg gcagataaag ctttgcattt tttcaaatgc ctgcgccctg ttctcttcac
      841 ccaatgtgtc gtgataaaag cctggcagca gatgcagctc tttcagaggg ctacgtaatc
      901 tctgataaaa atcaatctgc ggttggcgat gcaccacata gtcatcacct gatatcagaa
      961 gttgcgtggg gagcgtaatc gccgccgcat cgctaacaat acgttcagac gttttataga
     1021 gatcgagcaa gatattgacg gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg
     1081 ccacccgttg ccgatcgtgg gtcaaatagc gccctttcac ataggaatta ataaaaaata
     1141 gaccacgcag acgatgccat aacgccagcg caggacgtgc cagcggcaca tacaatttaa
     1201 ccttaaaggc cggagaagcc agcaccagcc cacgaattgc aggtgcataa tcatgaaccc
     1261 atgtggcaac aagcactgcg ccgacgcttt gcgcgatcac taccacctct tccagtccga
     1321 cctggctgtc gctggcagca aaacggacaa attcatcgac atcctgcact gagcgcataa
     1381 gagacgggct ataaccacgc ggccccgaat tttttccatg cccccgggca tcccaggcat
     1441 aaaaagcagt atcaggcatc gccagttcat caacgagatg ttgtagacga ccagaatgtt
     1501 catgcccgcg atgaaataag acgatgacct ttttcgcccc tggctgtaaa gtgggccagt
     1561 gccgataata caatgctgta ttatcactgg tggtaaaaaa atgttcccca gggatgcgtg
     1621 aattttccat tttctctatt ttccttcagt aacagcagta gcgaataaaa taaaaaaata
     1681 ccggagcggt gaaaattaat gagtcaatcc tgtcaaggag tccgccatgt cctagtaata
     1741 gttttccact atctttaaca ccaatatctc gtttgatggc tgacatcact acatcgccgc
     1801 aaaaaccact aataccaatt aacaaacccg ctaataatgc ctgtaatgta tttagcggtg
     1861 tcagcaacgg tccaataatc agtgacgcaa tcatggtggt gattacgccc cccagcagac
     1921 cttccagcgt ttttcccggg ctgactttgg ggaccacttt tcttctgccg caggatttcc
     1981 cccataaata ctgtgcaata tcgtttgact cggttaacgc caaaagaaat aacaccagta
     2041 acgccccgcc ctggatattt atggttggca acattaatag ccaggcggca tgactcaagg
     2101 caaaaacggt cattatccat ccccaaaaaa tggcgctcac ggtatgctga aaaccggagg
     2161 ggtcacccac aaaaactcgc caggtggcta atatcagaaa cccagtcaaa ggtataaata
     2221 acagaaacag ctcaaagcaa ttaaaaccga taagcaaata gttaagagga ataacccaat
     2281 ataaccaacg cgaaaaatgt acggatataa gcgtacaata ttctttcaat gccagaaaac
     2341 ttatcagcgt aaaaaacgtc aacgtcatcc agcgtgggcc tgaaatcacc agtgaaaaac
     2401 aaatgacgat gccccaccag gtacgaatac gtaatgtaac ttcatcccct tttctctcag
     2461 gtaaccgcca tagcagaaaa cggtttatca gcttgctaaa attaacaacg cgaaaagtgt
     2521 tgccagagat ttttccagca ttatctttca gcgctcataa gaagcacact ccgacaacga
     2581 ttaatcgcag tacagataag cagaattgaa gcaatgctcc ataaaagatt attccattgc
     2641 atccactgtg gataaatggc aacggccaga ccccacagac caaatattaa cgcgcgatca
     2701 cttttgccaa atggaccggc ataactgcga acaccgttaa tcgtctgcgc gagtaaaccg
     2761 caaaactcgg tcaatatggt gcaaaataac atgagtatca cgagtgatgc gttactttcc
     2821 ggtaaaaata aaaacggtaa atagagcgca atatcggaaa taacatcgcc agtctcattc
     2881 aaaatcgccc ccagacgtgt ttgctgattg cactcacgcg ccaacatgcc atccagcgca
     2941 ttgagcgcca tacggataaa aagcacgatg ggcaatagca aaaagaggat gggttgtgcc
     3001 accaacacca acagcaatcc ggtaaaaaga gaaagcgcca gtgccgtaag agtgatgtga
     3061 ttcgctgtaa cgtggtgctt ataaagccaa aacatcgtcg gccttaacag cgactgaaag
     3121 agcggtttta tctggtagag cgtcattatc aatccttgat aattcggttt gcgctatggt
     3181 gcatcaattc ccctgacgga gatatcgcaa cacgccgctt cattaaaaat tcagaatata
     3241 tcaaggtatt gggggctcac tgtcaggaat gattatcccc aataaatcaa gtagttaaaa
     3301 attattacgc tgttattcaa tacaactcat caagtttcct catcgcttgt tcccgtattg
     3361 cgcgcgtctt accgttaagc gccagctggc ggactaaatc cagttcaggg ttaacaagct
     3421 ctaacgccag caaaggatca cgtttcagcc tcgccttcaa tacatcaact ggaaagcggg
     3481 gattattcat cgccacaatc caccactcgg gatcaatttc tgccgccagt gccgcagagg
     3541 gggtgtggcg gttgctaagt gcgccgaact tgaaaaatgt gagattgctt ttcgccagtg
     3601 tactccacca tatacgatcc ggttgttttg ccagtacatc cagttgtgct aacgcatcgg
     3661 ggtcgagtgc ataataaaca cgtaatgctt catcaacctg cgcacgggag ctattttcct
     3721 gcctcagaaa aaacttcgcc caaatatcat tgcgtggcgt gttttgctgt aattccttga
     3781 gaacaacagc gggtaaatta tcccaactaa ctaatgcact ctctaaaaag tcccagtctt
     3841 ccaaacgtat cagggcggcg gcatttaata gcatctggtc atcaacttcg ccgtggctaa
     3901 tcagctcaga caaaatcata tcagcgctgg tttcatatgc atctgaatct gctttcggat
     3961 tatttttccg atgcgataaa gcacgttgcc aaattttttg tttcatcgac gtgctcagat
     4021 actgattttt cgccagatta ctccacccct caggatcgtt gaaacgtgtc aataaccgtt
     4081 gtaaagcagc gtcagagtga attacctgca tatcccgaca gcaaaagtag gcaggggatt
     4141 ttttctccat ccttttaaca aaattaaact gtaagttatc tggcaatgct aaaaataaca
     4201 cacgtattag atcatcgtca ttctcgaatt tttggtataa ataggctgca actttgtcac
     4261 tctcttgtgg cgtcatccgc aataattgac catggtgttt catctctgcc agcttgaacg
     4321 ctaagcttgt gacaacattg tttgatgaat cgttcgccaa ctgcattgcc tgattgtaag
     4381 tgagttgtcc tccagcaccg acctgacgaa tagaggaatc atcactgcgg atatagccat
     4441 caataatgga ttcgggcaat aaccacgcat ctttataaag ctcctgaatg atcatatccc
     4501 aatctctttt cgccagagtc atgacgatat ccagagggag attttcacgt agcgcaagat
     4561 tcaacggacc gacaccatca ggtagacgac tcattcgcgt cattatttcg acagagagcg
     4621 gttcacggtc gagcaacccc agaacaacag gctggtaacc caattgctga tcggtcaact
     4681 ccagaatctc attgtcaggc atatatctcg ccatactttc aatcgtttgt tcatcattgc
     4741 tattaataag ttgtttgcgt aggttagctg aaattccttt aagccttggg agagttcgca
     4801 ttatggcaag atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg ttgccttcat
     4861 ccagcatttg caattcctgc actaactcag gagaaggctt agggcgaaac agcgcttctt
     4921 ttttcatctg attacagaca ttccctgcca tgcgaatatt gcttcgctta aaaatttctt
     4981 tcagagcatc ttcatcattt attagacccc aataatatgc atcaggattt attatcatca
     5041 gcgctttttt gacattttca gtgtcatttt tgtcactcgc acggaccatg aattcactat
     5101 ttgtcgaaga cgcaacaaca ggtgcaatta tgtcattgat aaatacccca cgcgcattaa
     5161 tactcccctt aatcgtagac ggattatcgt aatatgagtc gataaatacc agtccacttg
     5221 catttatcga accggtaatt aacagtggtg aatccgtaac tatattcgtt gccgtcacgt
     5281 taccgagaat aacccatgga ttagaaagag agaaagtgga aatattacct ttgactgtca
     5341 aattgccaag aacaatcaat ttttgattac tctctatatt accttctacc actaaatcgc
     5401 cacgatgcac tttccagtcc gatgcagata aaccgaaggg gacaagattc acgctactgt
     5461 tgatagcttg ttcaacttgt tgttt
//
LOCUS       DAZPAB010000225         5483 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZPAB010000225 DAZPAB010000000
VERSION     DAZPAB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.226"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            240..2672
                     /gene="hsdR"
                     /locus_tag="ACHUPG_003654"
     CDS             240..2672
                     /gene="hsdR"
                     /locus_tag="ACHUPG_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFR0863081.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="ACHUPG_003655"
     CDS             2739..4208
                     /locus_tag="ACHUPG_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR0863082.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..>5483
                     /locus_tag="ACHUPG_003656"
     CDS             4208..>5483
                     /locus_tag="ACHUPG_003656"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFR0863083.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNS"
BASE COUNT         1617 a         1300 c         1311 g         1255 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttgttgtc ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt
     4801 ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa
     4861 agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga
     4921 aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct
     4981 ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac
     5041 cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac
     5101 cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact
     5161 tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga
     5221 aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag
     5281 cgatgaggaa aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat
     5341 agttaatatt aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc
     5401 acctgatatt attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc
     5461 aggagtcaaa gggccaaata gtc
//
LOCUS       DAZPAB010000226         5453 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZPAB010000226 DAZPAB010000000
VERSION     DAZPAB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.227"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACHUPG_003657"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACHUPG_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR0863084.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="ACHUPG_003658"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="ACHUPG_003658"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR0863085.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="ACHUPG_003659"
     CDS             complement(3736..4092)
                     /locus_tag="ACHUPG_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR0863086.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="ACHUPG_003660"
     CDS             complement(4096..4512)
                     /locus_tag="ACHUPG_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR0863087.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACHUPG_003661"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACHUPG_003661"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR0863088.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1274 a         1448 c         1435 g         1296 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gat
//
LOCUS       DAZPAB010000227         5398 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZPAB010000227 DAZPAB010000000
VERSION     DAZPAB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.228"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..497
                     /locus_tag="ACHUPG_003662"
     CDS             <1..497
                     /locus_tag="ACHUPG_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HFR0863089.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="ACHUPG_003663"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="ACHUPG_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="ACHUPG_003664"
     CDS             2500..3114
                     /locus_tag="ACHUPG_003664"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR0863090.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="ACHUPG_003665"
     CDS             3114..3443
                     /locus_tag="ACHUPG_003665"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR0863091.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="ACHUPG_003666"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="ACHUPG_003666"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR0863092.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="ACHUPG_003667"
     CDS             4529..4840
                     /locus_tag="ACHUPG_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR0863093.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4824..5373
                     /locus_tag="ACHUPG_003668"
                     /pseudo
     CDS             4824..5373
                     /locus_tag="ACHUPG_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1177 a         1344 c         1425 g         1452 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca
     5101 caaccttcag aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa
     5161 aagcggggct ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac
     5221 ccggcattac caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat
     5281 gctcgtctcg gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg
     5341 tttttggcga ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggca
//
LOCUS       DAZPAB010000228         5396 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZPAB010000228 DAZPAB010000000
VERSION     DAZPAB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.229"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            238..1296
                     /gene="citC"
                     /locus_tag="ACHUPG_003669"
     CDS             238..1296
                     /gene="citC"
                     /locus_tag="ACHUPG_003669"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR0863094.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1311..1607
                     /gene="citD"
                     /locus_tag="ACHUPG_003670"
     CDS             1311..1607
                     /gene="citD"
                     /locus_tag="ACHUPG_003670"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR0863095.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1604..2512
                     /gene="citE"
                     /locus_tag="ACHUPG_003671"
     CDS             1604..2512
                     /gene="citE"
                     /locus_tag="ACHUPG_003671"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR0863096.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2523..4055
                     /gene="citF"
                     /locus_tag="ACHUPG_003672"
     CDS             2523..4055
                     /gene="citF"
                     /locus_tag="ACHUPG_003672"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFR0863097.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4059..4609
                     /gene="citX"
                     /locus_tag="ACHUPG_003673"
                     /pseudo
     CDS             4059..4609
                     /gene="citX"
                     /locus_tag="ACHUPG_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4584..>5396
                     /gene="citG"
                     /locus_tag="ACHUPG_003674"
     CDS             4584..>5396
                     /gene="citG"
                     /locus_tag="ACHUPG_003674"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFR0863098.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIE"
BASE COUNT         1267 a         1523 c         1407 g         1199 t
ORIGIN      
        1 attacattaa ttaaaaaact taaaaccatt aaataaataa aaaaccacta actctatgtg
       61 aaataaatca aaatttcacg ccgaaatact ccttaggatg tatagcaaaa ggagaaaaag
      121 atatacctcg atcacccccc tttctcccaa gtgaaaataa aaggttatca gtttgcaaca
      181 ttgaacaaca ttcgttgcaa atcgataaca acatgcacct tcaggatact atttattatg
      241 ttcggcaatg atattttcac ccgcgtaaaa cgttcagaaa ataaaaaaat ggcggaaatc
      301 gcccaattcc tgcatgaaaa tgatttgagc gttgacacca cagtcgaagt atttattacc
      361 gtaacccgcg atgaaaagct tatcgcgtgc ggtggaattg ccggaaatat tattaaatgc
      421 gttgctatca gtgaatccgt tcgcggtgaa ggactggcgc tgacattagc cactgaactg
      481 ataaacctcg cctatgagcg gcacagcacg catctgttta tttataccaa aaccgaatac
      541 gaggcgctgt tccgccagtg cggtttttcc acgctgacct gcgtacccgg cgtgatggtg
      601 ctgatggaaa acagcgccac gcgactgaaa cgctatgccg aatcgctgaa aaaatttcgt
      661 catccaggga acaagattgg ctgcattgtg atgaacgcca atccctttac gaatggtcac
      721 cgttatctga ttcaacaagc tgcggcacag tgcgactggt tgcatctgtt tttagtcaaa
      781 gaagattctt cacgcttccc ctatgaagac cggctggatc tggtgttaaa aggcaccgcc
      841 gatattccac gcctgactgt gcatcgcggc tcagaataca tcatctcccg cgctacgttc
      901 ccttgctact tcattaaaga acagagcgtc attaaccatt gttacaccga aattgatctg
      961 aagattttcc gtcagtacct cgctcccgcg cttggtgtaa ctcaccgctt tgtcggtact
     1021 gaaccctttt gtcgcgttac cgcccagtac aaccaggata tgcgctactg gctggaaacg
     1081 ccgactatct ccgcagcgcc catcgaactg gttgaaattg agcggctgcg ttaccaggag
     1141 atgccgatat ccgcttcccg ggtacgtcaa ctgctggcga aaaacgatct cacggctatc
     1201 gcgccgctgg tccctgcagt cacgctgcat tatttgcaga acctgcttga gcactcccgc
     1261 caggacgcgg cagctcgtca aaagaccccc gcatgagaaa caggtgaaaa atgaaaataa
     1321 accagcccgc cgttgcaggc acccttgagt ctggggatgt gatgatacgc atcgccccac
     1381 tcgatacgca ggatatcgac ctgcaaatca atagcagcgt tgagaaacag tttggcgatg
     1441 caattcgcac caccattctg gacgttctcg cccgctacaa cgtgcgcggc gtacagctga
     1501 atgtcgatga caaaggcgca ctggactgca ttttacatgc acgactggaa gccctgctgg
     1561 cacgcgccag cggtatcccg gctctgccat gggaggattg ccaatgattt ccgcttcgct
     1621 gcaacaacgt aaaactcgca cccgccgcag catgttgttt gtgcctggtg ccaatgccgc
     1681 gatggtcagc aactccttca tctacccggc tgatgccctg atgtttgacc tcgaagactc
     1741 cgtggcatta cgtgaaaaag acaccgcccg ccgcatggtt tatcacgcgc tgcaacatcc
     1801 gctgtatcgc gatattgaaa ccattgtgcg tgtcaacgcg ctggattccg aatggggtgt
     1861 taacgacctg gaagccgtcg ttcgcggtgg tgcggacgtt gtgcgtctgc cgaaaaccga
     1921 taccgctcag gatgttctgg atattgaaaa agagatcctg cgtatagaaa aagcctgtgg
     1981 tcgtgaaccc ggcagcaccg gcctgctggc ggcgattgaa tctccgctgg gcattacccg
     2041 cgcagtggaa atcgctcacg cttccgagcg tttgatcggt atcgccctcg gtgcagaaga
     2101 ctatgtgcgc aacctgcgta cagaacgctc cccggaagga actgaactgc tgttcgcacg
     2161 ctgttccatt ttgcaggccg cgcgctctgc gggtattcag gcgttcgata ccgtctattc
     2221 cgacgctaac aacgaagccg gattcctgca agaagccgcc cacatcaaac agctgggctt
     2281 tgacggcaaa tcgctgatca acccgcgtca gattgatctg ctgcacaacc tctacgcacc
     2341 gacccagaaa gaagtggatc acgcccaccg cgtcgtagaa gccgctgaag ccgccgctcg
     2401 cgaaggcctc ggcgtggttt ccctgaacgg caagatggtg gacggtccgg ttatcgatcg
     2461 cgcccgtctg gtgctctccc gtgcagaact ttccggcatc cgcgaagaat aaggcaatca
     2521 aaatgacgca gaaaattgaa caatctcaac gacaagaacg ggtagcggcc tggaatcgtc
     2581 gcactgaatg cgatcttgcc gctttccaga actcaccaaa gcaaacctac caggctgaaa
     2641 aagcgcgcga tcgcaaactg tgcgccaacc tggaagaagc gattcgtcgc tcaggtttac
     2701 aggacggcat gacggtttcc ttccatcacg ctttccgtgg cggtgacctg accgtcaata
     2761 tggtgatgga cgtcatcgcg aagatgggct ttaaaaacct gaccctggcg tccagctccc
     2821 tgagtgattg ccatgcgccg ctggtagaac acattcgcca gggcgtggtt acccgcattt
     2881 atacctctgg cctacgcggt ccactggcgg aagagatctc ccgtggtctg ctggcggaac
     2941 cggtgcagat ccactctcac ggcggtcgtg tgcatctggt acagagcggc gaactgaata
     3001 tcgacgtggc tttcctcggc gtcccgtcct gtgatgaatt cggtaatgcc aacggctaca
     3061 ccggtaaagc ctgctgcggc tccctcggct atgcaatggt tgatgccgac aacgcaaaac
     3121 aggtcgtgat gcttaccgaa gaactgctgc cttatccgca taatccggca agcattgagc
     3181 aagatcaggt tgatttgatc gtcaaagtta atcgcgttgg cgatgctgca aaaatcggcg
     3241 ctggtactac ccgtatgacc actaacccgc gcgaactgct tattgcccgt agcgctgcgg
     3301 atgtgattgt caactctggc tacttcaaag aaggtttctc catgcaaacc ggcaccggcg
     3361 gcgcttcgct ggcggtaacc cgtttcctgg aagacaaaat gcgtagccgc gatattcgcg
     3421 ccgacttcgc cctcggcggt attaccgcga cgatggttga tctgcacgaa aaaggtctga
     3481 tccgcaaact gctggatgtg cagagctttg acagccatgc tgcgcaatcg ctggcccgta
     3541 accccaatca catcgaaatc agcgccaacc agtacgctaa ctggggttcg aaaggcgcat
     3601 cggttgatcg tctcgacgtg gtggtactga gcgcgctgga aattgacagg cagttcaacg
     3661 ttaacgtgct gaccggttct gacggcgtac tgcgtggcgc ttccggtggt cactgcgata
     3721 ccgcgattgc ctctgcgctt tccatcatcg tcgcgccact ggtacgcggt cgtattccga
     3781 ctctggtgga taacgtgctg acctgcatca ccccgggctc cagtgtcgat attctggtca
     3841 cagaccacgg tatcgcagtt aacccggcac gtccggaact agcagaacgt ctgcaggaag
     3901 cgggcattaa agtggtttcc attgagtggc tgcgcgaacg tgcgcgtctg ctgaccggtg
     3961 aaccacagcc gattgaattc accgaccgcg tcgttgccgt tgtgcgttac cgcgatggct
     4021 cggtgatcga tgttgtgcat caggtgaagg aataagccat gcacctgctt cctgaactcg
     4081 ccagccacca tgcggtgtca attcccgagc tgctcgtcag ccgggatgaa aggcaagcac
     4141 ggcaacacgc ctggctcaag caccatcctg ttccactggt ctcctttacc gtggttgcgc
     4201 ctgggccgat taaagacagc gaggtcacac gccgaatttt taatcatcgc gtgacagcct
     4261 tgcgtgcctt agccacaaag cagggctggc aaattcagga gcaggctgca ctggtttccg
     4321 ccagcgggcc ggagggcatg ttgagcattg ccgccccggc tcgcgacctc aagctcgcca
     4381 ccattgagct tgaacatagt catcctctcg ggcggttatg ggatatcgat gtcctgacgc
     4441 ccgaaggcga aattctctcc cgccgcgact attcactgcg cctcgccgct gcctgttgtg
     4501 cgagcaaagc gcagccgtct gcgcgcgtgg aaaaacccat caactgaccg atttactcaa
     4561 ccgcatggag gcactgctga acgatgtcga tgcctgcaac gtcaactaaa accacaaagc
     4621 ttgcgacgtc attaatcgat gagtacgccc tgctgggctg gcgcgccatg ctgactgaag
     4681 tcaatctgtc accgaaacca ggcctcgtgg atcgcattaa ctgcggtgcg cacaaagata
     4741 tggcgctgga agatttccac cgcagcgcgc tggcgattca gggctggtta ccccgtttca
     4801 ttgaatttgg tgcctgtagt gcagaaatgg caccggaagc ggtactccac ggattacgcc
     4861 caattggtat ggcttgcgaa ggtgatatgt tccgcgccac tgcgggcgta aacacgcata
     4921 aaggcagcat tttttcttta gggctgctat gtgcggcaat tggccgtttg cttcagctca
     4981 accagccggt tacgccaaca accgtttgtt ctacggcggc aagtttctgc cgtggcctga
     5041 ccgatcgcga actgcgtacc aataattcac gactgacggc aggtcaacgg ttgtaccagc
     5101 agcttggcct taccggcgca cgcggtgaag ccgaagcagg ttatccactg gtgatcaacc
     5161 acgccttgcc gcattacctc actctgctgg atcaggggtt agatcctgaa ctggcattgc
     5221 tcgatacctt gctcctactg atggctacca acggcgatac caacgttgca tcgcgcggtg
     5281 gcgagggggg cctgcgctgg ctacagcgcg aggcgcaaac attattgcaa aaagggggca
     5341 ttcgaacccc caccgatctc gattatctcc ggcagttcga cagggagtgt atcgaa
//
LOCUS       DAZPAB010000229         5395 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZPAB010000229 DAZPAB010000000
VERSION     DAZPAB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.230"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            99..2135
                     /gene="yihQ"
                     /locus_tag="ACHUPG_003675"
     CDS             99..2135
                     /gene="yihQ"
                     /locus_tag="ACHUPG_003675"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFR0863099.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            2181..3555
                     /locus_tag="ACHUPG_003676"
                     /pseudo
     CDS             2181..3555
                     /locus_tag="ACHUPG_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            3598..5001
                     /locus_tag="ACHUPG_003677"
     CDS             3598..5001
                     /locus_tag="ACHUPG_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0863100.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            5069..>5395
                     /locus_tag="ACHUPG_003678"
     CDS             5069..>5395
                     /locus_tag="ACHUPG_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFR0863101.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         1275 a         1324 c         1354 g         1442 t
ORIGIN      
        1 gtaaagtttt tatatatcag ttttatgaga atcattttac ataagcctgt aataacagga
       61 aggcaacaat ctcgttctca actctatgga gattagttat ggatacgcca cgtccacagt
      121 tattagattt tcaatttcat cagaataacg acagttttac cctacatttt caacaacgtc
      181 ttattttaac ccataacaaa gataatcctt gtttatggat tggctcaggt atagcggata
      241 tcgatatgtt ccgcggtaat ttcagcatta aagataaact acaggagaaa attgcgctta
      301 ccgacgccat cgtcagccag tcaccggatg gttggttaat tcatttcagc cgtggttctg
      361 acattagcgc cacgctgaat atctctgccg acgatcaggg ccgtttattg ctggaactac
      421 aaaacgacaa ccttaaccac aaccgtatct ggctgcgcct tgccgctcaa ccagaggacc
      481 atatctacgg ctgcggcgaa cagttttcct acttcgatct gcgcggcaaa ccgttcccgc
      541 tatggaccag tgaacaaggc gttggtcgca acaaacaaac ctatgtcacc tggcaggccg
      601 actgcaaaga aaatgcgggc ggcgactatt actggacttt cttcccacag cctacgtttg
      661 tcagcacgca gaagtattac tgccatgttg ataacagttg ctacatgaac ttcgacttta
      721 gcgccccgga ataccatgaa ctggcgctgt gggaagacaa agctacgcta cgttttgaat
      781 gtgctgacac atacatctcc ctgctggaaa aattaactgc cctgctggga cgccagccag
      841 aactgcccga ctggatttat gacggggtaa cgctcggcat tcagggcggg acggaagtgt
      901 gccagaagaa actggacacc atgcgtaacg cgggcgtgaa ggttaacggc atctgggcgc
      961 aggactggtc tgggatccgt ataacctctt ttggcaagcg cgtgatgtgg aactggaagt
     1021 ggaacagcga aaactacccg caactggatt cacgcattaa gcagtggtat caggagggcg
     1081 tgcagttcct ggcctatatc aacccgtatg ttgccagcga taaagatctc tgcgaagaag
     1141 cggcacaaca cggctatcta gcaaaagatg cctctggcgg tgactatctg gtggagtttg
     1201 gcgagtttta cggcggcgtt gtcgatctca ctaatccaga agcctacgcc tggttcaagg
     1261 aagtgatcaa aaagaacatg attgaactcg gctgcggcgg ctggatggct gacttcggcg
     1321 agtatctgcc caccgacacg tacctgcata acggcgtcag tgccgaaatt atgcataacg
     1381 cctggcctgc gctgtgggcg aagtgtaact acgaagccct tgaagaaacg ggcaagctcg
     1441 gcgagatcct cttctttatg cgcgccggtt ctaccggtag ccagaaatac tccaccatga
     1501 tgtgggcggg cgaccagaac gtggactgga gtctcgacga tggcctggcg tccgtagtcc
     1561 cggcggcgct gtcgctggca atgaccggac atggtctgca tcacagcgac attggcggtt
     1621 acaccaccct gtttgagatg aagcgcagca aagagctgct gctgcgctgg tgcgatttca
     1681 gcgccttcac gccgatgatg cgcacccacg aaggtaaccg tcctggcgac aactggcagt
     1741 ttgacggcga tgcggaaact atcgcccatt ttgcccgtat gactaccgtt ttcaccaccc
     1801 tgaaacctta tctgaaagag gccgtcgcgc tgaatgcgaa gtccggtctg ccggtcatgc
     1861 gcccgctgtt cctgcattac gaagacgatg cacataccta ttccctgaaa tatcagtacc
     1921 tgttaggccg cgacattctg gtcgctccgg tacacgaaga aggtcgcagc gactggacgc
     1981 tctatctgcc ggaggataac tgggtacatg cctggacggg tgaagcgttc cggggcgggg
     2041 aagttaccgt taatgcgccc atcggcaagc cgccggtctt ttatcgcgcc gatagcgaat
     2101 gggcggcact gttcgcgtcg ttaaacagca tctaatcaca tccgcccgtg gggaaactca
     2161 tgggcaaagg gagaacatcg atgagtcaca tcacaacgga agatccagca actttacgcc
     2221 tgccctttaa agagaaactc tcttacggta ttggcgacct ggcctctaac atcctgctgg
     2281 atatcggtac gctttatctt ttgaagtttt ataccgacgt tctggggctg ccaggcacct
     2341 atggcggcat tatctttttg atttcaaaat tctttactgc gtttaccgat atgggtaccg
     2401 gcattatgtt ggattcccga cgcaagatcg gtccaaaagg taagttccgt ccttttattc
     2461 tgtatgcgtc attcccggtc accttactgg cgatcgccaa ctttgtcggc acaccgtttg
     2521 atgtcaccgg taaaacggtg atggccacta ttctgtttat gctttacgga ctgtttttca
     2581 gcatgatgaa ctgctcctac ggcgcaatgg tcccggcaat caccaaaaac cccaacgaac
     2641 gcgcctcact ggcggcatgg cgtcagggcg gcgctacgct gggcctgctg ctgtgcacgg
     2701 tgggattcgt gccagttatg aatcttatcg aaggtaatca gaaacttggc tatatcttcg
     2761 ccgccacgct gttttcactg ttcggcctgc tgtttatgtg gatctgctac tcaggcgtga
     2821 aagagcgtta tgtcgaaacc caaccaacca atccggcgca aaagcctggc ctgttgcaat
     2881 ctttccgcgc aattgccggt aaccgcccac tgttcattct gtgtattgcc aacctctgca
     2941 ccttaggggc gtttaacgtc aagctcgcca ttcaggtcta ttacacccag tacgtactta
     3001 acgatcccat cctgttgtcg tatatgggat ttttcagcat gggctgtatt ttcatcggcg
     3061 tgttcctgat gcctggcgca gtcagacgtt ttggtaagaa gaaggtctat atcggcggcc
     3121 tgctgatttg ggtgctgggc gatctgctca actatttctt cggcggcggc tcggtcagct
     3181 ttgtggcgtt ctcctgcctg gcgttcttcg gctcagcgcc tgaactgggc gctggtttcc
     3241 gacaccgtcg agtacggcga gtggcgcacc ggcgtgcgtt cggaaggcac ggtctacacc
     3301 ggcttcacct tctttcgcaa agtgtctcag gcgctggctg gtttcttccc cggctggatg
     3361 ctgacgcaaa ttggctatgt gccgaacgtc gcacaggctg accacaccat tgaagggtta
     3421 cgccagttga tcttcatcta cccaagcgca ctggcggtag tcaccattgt ggcgatgggt
     3481 tgcttctaca gcctgaacga gaagatgtat gtccgcattg tggaagagat agaagcccgt
     3541 aaacgcacgg cgtaataata ataaacgacg ccctgcgggg cgttataagg agtgattatg
     3601 tctgaccata atccactgac attaaaactg aatctgcggg aaaaaatcgc ctatggtatg
     3661 ggcgacgtcg gttcgaattt aatgctctgc atcggtactc tgtatctcct caaactttat
     3721 accgatgaat tagggatgcc tgcttactat ggcgggatta tctttctggt cgcgaagttt
     3781 tttaccgcgt ttaccgatat gctcaccgga tttttactcg actcgcggaa aaatattggg
     3841 cctaaaggca aatttcgtcc ctttatttta tatgcagcag ttccggcggc gttaattgca
     3901 acgctccagt ttatcgccac caccttttgt ttgccggtta aaacgacgat tgccaccacg
     3961 ctgtttatga tgtttggact ttcatacagt ctgatgaact gctcgtatgg tgcgatgatc
     4021 ccggcaatta ccaaaaaccc gaatgagcgc gcgcagctcg cggcttaccg ccagggtggt
     4081 gcaaccatag ggctattgat ttgtaccgtg gcgtttattc cgttgcagtc gcttttttct
     4141 gactcaaccg tcggttatgc ctgtgcggca cttatgttct ccattggcgg ctttattttt
     4201 atgatgctgt gctacagagg cgtcaaagag cattatgtgg acacagcgcc aaccggacat
     4261 aaagccagta ttctcaaatc tttttgcgcg atatttcgta atccaccatt gctggtttta
     4321 tgcattgcta acctgtgtac tctggcggca tttaatatca aactggcgat tcaggtctat
     4381 tacacccagt atgtgctgaa tgatattaat ttattgtcgt ggatgggatt cttcagtatg
     4441 ggatgcatcc tcgtcggcgt attactggtg ccagtaactg taaaatgttt tggtaaaaaa
     4501 caggtttatt tagctggcat ggtgctatgg gcggtgggtg atatactgaa ttatttctgg
     4561 ggaagtaact ctttcacttt cgtcatgttc tcttgtgtcg ccttttttgg cacggccttt
     4621 gtaaacagcc tgaactgggc actggtacca gataccgtag attacggtga atggaaaacc
     4681 ggtattcgtg ccgaaggttc tgtttatacc ggttatacct tctttcgtaa aatttctgcc
     4741 gcacttgctg gcttcttgcc aggcattatg ctgacgcaaa ttggttatgt tcccaacatc
     4801 gcgcaaagcg atgcgacatt acagggtttg cgtcagctca tttttatctg gccttgtgca
     4861 ctggcaatta ttgctgcatt gacgatggga ttcttttaca cactcaatga aaaacggttt
     4921 gcattgatta ttgaggaaat caaccaacgc aaaaataaag agatggcgac agaaaaaaaa
     4981 acggcttccg taacgttata aatgtcaata gccgctattt ccatcctggt gggtggcggc
     5041 ctccctacgt ttaaaaaatg gacttattat gaaaaagatt aatgcaataa ttttattatc
     5101 ttctctgacc tctgcctcgg tatttgctgg cgcatacgtc gaaaacaggg aggcatataa
     5161 tcttgcctcc gatcagggcg aggttatgct acgtgtgggt tataacttcg atatgggcgc
     5221 gggtattatg ttaaccaata cctacacctt tcagcgagaa gatgaactaa aacatggcta
     5281 taatgaaatt gaaggttggt atcctttatt caaaccaacc gataaattaa ccatccagcc
     5341 cggtggctta attaatgata agagtatcag ttcgggtggt gcagtgtatc tggac
//
LOCUS       DAZPAB010000230         5374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZPAB010000230 DAZPAB010000000
VERSION     DAZPAB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.231"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(99..581)
                     /locus_tag="ACHUPG_003679"
     CDS             complement(99..581)
                     /locus_tag="ACHUPG_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863102.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(649..2598)
                     /locus_tag="ACHUPG_003680"
     CDS             complement(649..2598)
                     /locus_tag="ACHUPG_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR0863103.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2683..3018)
                     /locus_tag="ACHUPG_003681"
     CDS             complement(2683..3018)
                     /locus_tag="ACHUPG_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR0863104.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(3018..3374)
                     /locus_tag="ACHUPG_003682"
     CDS             complement(3018..3374)
                     /locus_tag="ACHUPG_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR0863105.1"
                     /translation="MAKIAGICFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3374..4870)
                     /locus_tag="ACHUPG_003683"
     CDS             complement(3374..4870)
                     /locus_tag="ACHUPG_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR0863106.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVEKYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4867..5028)
                     /locus_tag="ACHUPG_003684"
     CDS             complement(4867..5028)
                     /locus_tag="ACHUPG_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFR0863107.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(5038..>5374)
                     /locus_tag="ACHUPG_003685"
     CDS             complement(5038..>5374)
                     /locus_tag="ACHUPG_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863108.1"
                     /translation="RDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTLL
                     DLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEH
                     HLEMRFPQN"
BASE COUNT         1284 a         1565 c         1155 g         1370 t
ORIGIN      
        1 cagtaagttg gagtcattac caagaaagag aaggtaaaga aggtaagtcc atactgtctc
       61 cactataaaa aaaccgccaa tttctggcgg cttatatatt aatcatcatt aatatctttc
      121 atattcaatt tgcaattttc gtaggtcact cccgactcgc cattaacatt aactccagct
      181 ctgatattcc cgttttcttt tacatctaca aaaaactggc ctcttggcat cttgaacgca
      241 aatgtaaaac caacagtatc tgaatcaaaa tcatcatctt tatatattgg gatcaaaaat
      301 gtcatcgtcg ctggtgatgc attgtttgca aaatcaaata cacctatact tttgccgaat
      361 tcataagaag cttttctcaa tataatcgca ccgctggcag aaggggatgg gcacgatatc
      421 ctcagttgtc tgaatccatc tgtatccaag tcaacgtcat tatctgcaga tgttttaacg
      481 ttactaataa atcgtgaaac aatctcctca tttgttagta ttttcgcctg agcacagcaa
      541 gaggttaata ttgcaaccgt cattaaaaat ggctttatca caaaacaaac tccattgtta
      601 gcatgttacg cgataatttt gcctcatctt tcaccacaaa aaaagaactc accttttaaa
      661 tggagaatac ccaacatcat gggtgatttt cataaaggga tcgcctgttt tcggcaaatc
      721 gataacgcgc attccaggcg gcgcgttgtc aaacgtcacc ttaagttcgc tgcgggttgc
      781 cggtgaagat gcaaggctta acagctgatt cctttccagt gatacattcg gggtgtagcg
      841 tttatcggga gataaatacc catcaccacg tggcatctgc catccggtca tgtcatagac
      901 aaaatcatgg aactgtttgc cccactcgtc gaacttatca ttcagaccaa atccactgtt
      961 cagtgcatca gccacaaaat ttttaacaag ccagggatgt tctttttcaa actcctgcgc
     1021 catcatgccc agttcaagca atccaccaat cagactgatc tttccgaggc cctttaattt
     1081 cagccctgtt tttcctgccg ccgttgtcca ttgattctgg gcgactgttg ccgcacgaat
     1141 acttgccacc gccttcacac caatatacag ggcagaaacc gttgctattg tttttactgc
     1201 ctgttcccat ccccccattg ctctgacaac ctgatcaacc tcctgccaga cttttttcac
     1261 aaccggaccg accgtttccc agttatcaat tatgagatat gcaccaccga cgagaagcgc
     1321 aattaatccc tttgccggcg tcatattcat cacgctaccc atgatttttg ttatgcgtgt
     1381 caatgtgcca atcgccacgc ccatcgtcag taatgccgca ccagttttcg cgatggtttt
     1441 tacgacctca ggattttctc tgacaaacgt gcgcacttct tccagaaaag gcttcatctc
     1501 ttttatgcct tcattgagtg aaggcaggaa ggtttccccc agcgtggaag aaatcgcatt
     1561 gatctggttc tgcagcagca acaactgatt ttctgtcgtc gcggcacggg cggcatattc
     1621 tttctgcatt gagccgccat actgctgcgc atccgccaca cgattaaagt tggttcgcaa
     1681 caaatccaga ttcgtgagca gcggcgcaat ggcccccagg gattccttcc cgaacagcgc
     1741 tttcagcaca gcagcctgtt tttctttcgg cacttttgcc agagaatcca gtacgtgcag
     1801 catggccccg cgggcgtctt tctgcatatc cgccgccagt tttttcgggc taatgcgcaa
     1861 cgagcggagc acttttttct gcgatttggt ggcagaatcc ctcgctgtca gcgacaacat
     1921 aaaattcttt atccccgtcg cagcaatttc tgactccacc cccattccgg caatggttgc
     1981 ccccattgcg gcaatctctc cggaggccac acccgcaaca ctgcctaaag ggccaatacg
     2041 ggtcacaaca tcagaaatct ttttcgcact tgcaggaccg gtattgccaa gatagttaat
     2101 cttatccgca agccctgcca cctctccctg tgtcagttta aaggcagtgc gccactgcgc
     2161 catcatctga ccggattctt ctgccgtggt gtcaaaggcc acgcccatct tcacggcatc
     2221 gtcagtaaac tgcatcagct cgtcacgggc gataccagcc tgaccacccg ccgccacaat
     2281 ttcggcgata ccttccgccg acatgggcag ttctgttgac aggtcgcgca cctgctccgt
     2341 cattgcctta aacgcttccg gcgtatccag accatccacc actttccgga catccgccat
     2401 ttttgattca agggcaatgg cagatttgac cgggagcgcc agcgccccca gtactgcggt
     2461 tccggcaccc gctgcgccca gtgacaggct ggcaaactcc tttttaaaac ctttcagctg
     2521 gcgctgcatt cccttcagcg gtgccgatac cttatccacg gcagtgatga tcgccttcag
     2581 ctgaaaactg tcagccatgc ttcatctcct cgttaatgcg gacggcctcg gcttccagtt
     2641 ctgtaaactg cgaaacagcc acccgtcgca gctccagtgg attcagtttc cagaaccagg
     2701 caacattgta gagtcgtttc cggaggtgct tcccgtctcc gactgggtaa aaaaacgcag
     2761 gatctgcatg ctggtcttaa aaatatccag ttttgccatc tgcgccgccg atgaacacgg
     2821 gatccccgcc agcaacggga tatatttcag tgccacctgg ctgtccagtt taatactgcc
     2881 ttcaccggaa ataatgaaag gaaaacccag cgcctcgatt tcgtcatacg tgggctcgcg
     2941 caactccagc acatgcagca tttcgttatg cgctgtcacc ggttttttta aaactatttc
     3001 tgtaacgttc attgccatta ctgataccct ccctcttcgc cgtggaattc aagatctgcc
     3061 gtgccttctt cggcattatg attagcctct ccgtgcagcc atgccgccga aagcacatac
     3121 accatgccgt ttgccagctc agcggtaatc gtcatctggt ctgaggtggt aattttgtcg
     3181 accggaaagt ttttgggcac tttaaacgtg cccttaacgt aaggtgaccg ccaggtctcc
     3241 ttgtaatcca catcccctgc catgccgacg atgtcatccc ggacattggt gttcatcggc
     3301 acttcaatgc cacctgtcag cgataactgc tgaccatcca ctttaaaaaa acagattccg
     3361 gcgatctttg ccattatgct gactcctctg aatactgaag acggaactgg ttaaccaccg
     3421 caaagacacg taactgatta acataatccg gcgggaacag cgtattcagt cggttcgggt
     3481 tatctgcatc acgctcaact atcagatact gtttaaacag atcgtatttt tccacaatac
     3541 cggcacgctc catctgacga tatgtcgcca gaagttcccc tttgataacg gcaggagtga
     3601 caatcgcctg ccccggacca aaacgggtac catcatttgc cagcttgtga cgtccgtact
     3661 tgcttgtgat gaccgatttc agtttgcgaa gaacatatgc actggtatgc agagtttcac
     3721 tgtccagata gctgttgtct gccacgccat acgcattctt tttgtaggtg gttacggaac
     3781 gctggatctg caacgtgccg ccttccacat aagccgtcgc cacgccgtga gataaaaggg
     3841 tctgctgctc tgtcatgatg aatcgcttac ctttcggtgc cggaagcatc cccaccagct
     3901 cccccgtctg tgtcggacgg gcaggatcat tccggataaa taccgcttca cgggcaaggc
     3961 gactggcaac cagttcatcg acaggcgact gggtttcttt ttcgtaaccg gcaagcgtga
     4021 tatgttgctg attatgcata tctccggcac caaccagctc tgacagcgtt cccagttttg
     4081 cggtatagac atgcccgtat aactggcgcg cataactcca gcgaccgctg ctgtcattca
     4141 tttcggtcat catcatattg atggaggcgg catcgttgaa cggcagaccg ataaagtcga
     4201 atgcctcatc gcccatagca gcaacagcgg cggtaagatc aggcgctccg ctaccggcag
     4261 caccgacttc cgtcaccacc cgaagccctg ctggcaaaat ctcaccacca ccagaaccat
     4321 aataattcag acagaccggt aactcattac catacagccc cttatgacgg gcagtcagcg
     4381 tcaccacgcc tgcatcagat gacgccgtaa acggcagggt gataaccccg tttaccgctt
     4441 ccttaatcgc ggtggcaacc gcagtggcat catcgccatt caccacaggc acctgtacac
     4501 gggagcgccc gacatacaga ctcagggtgc cgctttcctc tgcttctccg gcaaccgtca
     4561 ccctgaccgt cgccgccgcc cctctggctt ccggtactgc aataacatac agttcaccga
     4621 aaggatctgt ctgacggtag acttccacca tacgcgccag ctggctcccc gccccacaaa
     4681 tctggcgggc ataatctgcc gatggcatca gcaccaggct gttaacctca atggctgcat
     4741 cgttgctggc atgcccgatc agtaatgcag gcgcgctggt caccgctgta tttgccgcag
     4801 aattgttcat ctcggcgtag aacaacggca ccagcgtatt cgacggaaca gtattaaaac
     4861 ttatcgtcat gattttttag ccttatcctg tttaacacgt accacatcac ccgccgctat
     4921 acggcggaac cagtagctgc tttcttccac atttcgccct tcggaaggca aaaggtctcc
     4981 acgggcaggg tcaggaaccg accgcccttt caggggtttc acaaacatga gggactctta
     5041 attctgaggg aaacgcattt ccagatggtg ctcaatgtca ccatccgggc cagtaccagg
     5101 atcgatgtaa tccacatcaa tactcagcag ggataaatcc ggcagggcat tcacgtcctc
     5161 tgcctgtcgc gtgtcttctt ccgtgatttc atacttcgcc gtaaaatcaa actggtaata
     5221 cagttcgtaa cggttcagat ccagcagggt gccaccgaca taaacaatct cgcccccttg
     5281 cgggtccggc atccatccaa gcagggcttt ccagagttct ctccggacat catgaacggc
     5341 gtcataggct gcccactgcc ctttttcatc acgc
//
LOCUS       DAZPAB010000231         5346 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZPAB010000231 DAZPAB010000000
VERSION     DAZPAB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.232"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            157..246
                     /locus_tag="ACHUPG_003686"
                     /pseudo
     CDS             157..246
                     /locus_tag="ACHUPG_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
     gene            548..1543
                     /gene="pgl"
                     /locus_tag="ACHUPG_003687"
     CDS             548..1543
                     /gene="pgl"
                     /locus_tag="ACHUPG_003687"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR0863109.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1584..2537)
                     /locus_tag="ACHUPG_003688"
                     /pseudo
     CDS             complement(1584..2537)
                     /locus_tag="ACHUPG_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2721..3773
                     /locus_tag="ACHUPG_003689"
     CDS             2721..3773
                     /locus_tag="ACHUPG_003689"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFR0863110.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3849..5282
                     /locus_tag="ACHUPG_003690"
     CDS             3849..5282
                     /locus_tag="ACHUPG_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFR0863111.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
BASE COUNT         1311 a         1197 c         1333 g         1505 t
ORIGIN      
        1 ttagtaatga tccaaatcat atctcaatgt actgtttttt tctttaaaaa aacagtacat
       61 tctattttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
      121 ttttgacgca gtgtcttgtt aacaggggga ttgaccatgc ttattggcta tgtacgcgta
      181 tcaacaaatg accagaatac cgatctacaa cgtaatgcgt ttatttgtgc cggaagtgag
      241 ttgattgttg cgcgaatggg aagttggcca gaagttgtta tggcatagtg tttacggtcg
      301 ttgatacagg taaacaaaag cggggagagt catactccct gaatagtcgc gaaaatatct
      361 gtggcattgt caaattcgtg tcggtgacta atattttagt aatatgccat tcgaaatgga
      421 gtgctaactt attgatttaa ttgatgtgcg atattgtatt ttatcgtgtt ttgttgtctg
      481 ttttttatcc agtaagtgtt gttatatgta tgaaagataa gggatttatc atttttgagg
      541 tgtgttcatg aagcaaacag tttatatcgc cagccctgag agccagcaaa ttcacgtctg
      601 gaacctgaat catgaaggcg cactgacgct gacacaggtt gtcgatgtgc cggggcaggt
      661 gcagccgatg gtggtcagcc cggacaaacg ttatctctat gttggtgttc gccctgagtt
      721 tcgcgtcctg gcgtatcgta ttgccccgga cgatggcgca ctgacctttg ccgcagagtc
      781 tgtgctgccg ggtagtccga cgcatatttc caccgatcac caggggcagt ttgtctttgt
      841 aggttcttac aatgcgggta acgtgagcgt aacgcgtctg gaagatggcc tgccagtggg
      901 cgtcgtcgat gtagtcgagg ggctggacgg ttgccattcc gccaatatct caccggacaa
      961 ccgtacgctg tgggttccgg cattaaagca ggatcgcatt tgcctgttta cggtcagcga
     1021 tgatggtcat ctggtggcgc aggaccctgc ggaagtgact accgttgaag gggccggccc
     1081 gcgtcatatg gtattccatc caaacgaaca atatgcgtat tgcgtcaatg agttaaacag
     1141 ctcagtggat gtctgggaac tgaaagatcc gcacggcaat atcgaatgtg tccagacgct
     1201 ggatatgatg ccggaaaact tctccgacac ccgttgggcg gctgatattc atatcacccc
     1261 ggatggtcgc catttatacg cctgcgaccg taccgccagt ctgattaccg ttttcagcgt
     1321 ttcggaagat ggcagcgtgt tgagtaaaga aggcttccag ccaacggaaa cccagccgcg
     1381 cggcttcaat gttgattaca gcggcaagta tctgattgcc gctgggcaaa aatctcacca
     1441 catctcggta tacgaaattg ttggcgagca ggggctactg catgaaaaag gccgctatgc
     1501 ggtcgggcag ggaccaatgt gggtggtggt taacgcacac taaccgctga tttaaccggc
     1561 gcagtctctc ctgcgccggt gtattaactt atctcctgta acgcatgtct ctggcgttcg
     1621 acgatattgg tccacaaatt gtctttatcg tcagtccaca aattaatcag caaggaaaaa
     1681 aagcgttcag ctgccggaga aagcacggca tctttgcggc gaataatccc caatgtccga
     1741 cgtataacgg gttccaccag tgggatacca ataatcgaag aatagggcgc atggggcatt
     1801 gccagaccag gaagcgccga aatccccagt cctgcctcca ctagtcctaa tgacgtcgaa
     1861 agatgacgca cttcgtaaaa ccagtccagc ttccagggct tgtcggctag ctgttgttct
     1921 atcaacaatc ggttgccgct ggaggaacgt acgccaatca ttttgtaacc cactaactcc
     1981 tgccattcta caagctactt tttggccaat ggatggtcac gccgacaggc cagcacgaat
     2041 ggctcgttga ccagtggggt aaaatcaatg gatgaatttg tgacgttgtt catgtttatg
     2101 ccaaagtctg attcgttaca cagcaccgat tccatgcaat tattcgtgcc ttgttccaga
     2161 atccgcactt taatattggg atacagctca ttaaatttac cgattgccag cggtaaaaaa
     2221 taaaatactg cggtcggaat acacgctaat gtcaccatac cacgatgata agcattcata
     2281 tcacgaatat taaaaagcgt ttcatcgaat ttctttatta attcccgcgc ctccgggagc
     2341 aacctttttc ctgcttttgt taacgtaacc ttgcgagttg tacgctcaaa tagttgaata
     2401 tgtaaatctt cctccatctt ttttattctt cgcgttaatg caggttgcgt aatattgagt
     2461 aatttagcgg cattgttaaa tgaactggac tctgccagta tgacaaatgc cttcatactt
     2521 gataattcat gcttcattat tactccggaa aatggaagcg acgattttgg ggggctggtc
     2581 gttaaaaatt ttaactgcat ttagccaact taaattaatg aaaaaatgtt attaatcgtt
     2641 gagctaaagt cattagagat gctttgtcct taatgtaacc atatcgcaat aagttatgtt
     2701 tttaaattga gggcattatt atgaaaaaaa taccctgcgt gatgatgcga ggtggaacct
     2761 cgaggggcgc gttcctgtta gcggaacatt tacccgaaga tcaaacgcag cgcgataaaa
     2821 tattgatggc aattatgggt tccggtaacg atctggaaat tgacggtatt ggcggcggta
     2881 atccactgac cagtaaagtc gccattatta gccgttccag cgatccgcgt gctgatgtcg
     2941 attatctgtt tgctcaagtt atcgtccatg agcaacgtgt cgataccacg cctaactgcg
     3001 gcaatatgct gtctggcgta ggggcattcg ccattgaaaa tggtttgatt gcagcgactt
     3061 cgccagttac ccgtgtacgt atccgcaacg tcaatacggg tacgttcatc gaagctgatg
     3121 tgcaaacgcc aaatggtgtt gtcgagtacg agggtagcgc cagaattgac ggcgtaccgg
     3181 gtactgccgc accggttgcg ctcactttcc tgaatgccgc tggaacgaaa actggaaaag
     3241 ttttcccgac tgataatcag attgattatt ttgacgatgt cccggtgacc tgtatcgata
     3301 tggcgatgcc agtcgtcatt attccggctg aatatctggg aaaaacaggt tatgaattac
     3361 cggcggaact ggatgccgac aaagcattat tagcccgcat tgaatctatc cggctacaag
     3421 cgggtaaagc aatgggctta ggtgatgtca gtaatatggt tatccctaaa cctgtgctta
     3481 tttctccagc gcagaaaggc ggggcaatta atgtgcgtta ttttatgccg cattcttgcc
     3541 atcgcgcgct ggcgataacc ggtgctattg ctatttccag tagttgtgca ttggaaggca
     3601 ccgtcacccg acaaatcgtc ccttctgtag gatatggcaa tatcaatatt gaacacccca
     3661 gtggtgcgct cgacgttcat ttaagtaatg aaggtcagga tgccacgacg ttacgcgcat
     3721 ctgttattcg gacaaccaga aaaatatttt ccggtgaagt ttatcttccc tgaaaaaatt
     3781 cgttgtcagg ataaggacaa tcaataaagg acttctgtat gagtcataca gaaagaacag
     3841 gattttaaat gaataagaaa tcgttatgga agctaattct gatattagcg atcccatgta
     3901 ttattggttt tatgccagct ccggcaggat taagcgaact ggcgtgggtg ctttttggta
     3961 tttacctggc ggccattgtg ggtctggtta tcaagccttt cccggaacct gtcgtactgt
     4021 taattgccgt tgctgcctca atggtggtgg tcggtaactt atccgacggt gcgtttaaga
     4081 ccaccgccgt attaagcggt tactcttcag gtactacctg gctggtgttc tcggcgttta
     4141 ccttaagcgc cgcatttgtg accaccggtt taggtaaact tattgcctat ctgctgattg
     4201 gtaaaatcgg taacaccatg ctgggtctgg gttacgttac ggtattcctc gatctggtac
     4261 tggctccggc aacaccgtct aacaccgcgc gtgcgggcgg cattgtgtta ccgatcatca
     4321 acagcgtggc ggtggctttg gggtccgaac cggaaaaaag tccgcgtcgt gtcggacatt
     4381 acctgatgat gtccatttac atggtcacca aaaccaccag ctatatgttc tttaccgcaa
     4441 tggcggggaa cattctggcg ctgaaaatga tcaacgacat tctgcacctg caaattagct
     4501 ggggtggatg ggcgctggcc gccggattgc cgggcatcat tatgctgctg gtcaccccgc
     4561 tggtaattta caccatgtat ccaccagaaa ttaaaaaggt ggataacaaa accatcgcta
     4621 aagcgggcct tgccgaactg ggaccgatga aaatccgcga aaaaatgctg ctcggtgtct
     4681 ttgtgctggc gctactgggc tggattttca gtaagtctct gggggttgat gaatccactg
     4741 tggcaatcgt tgttatggcg accatgctgc tgctgggtat cgttacctgg gaagacgtgg
     4801 ttaaaaataa aggcggctgg aataccttaa tctggtacgg cggtattatc ggcttaagct
     4861 ccttattatc gaaagttaaa tttttcgaat ggttagctga agtctttaaa aataacctgg
     4921 catttgatgg tcacggtaac gttgctttct tcgttattat tttcctcagc atcatcgtgc
     4981 gttatttctt cgcttccggt agtgcctata tcgttgctat gttacctgta tttgccatgc
     5041 tggcgaacgt ctccggcgca ccgttaatgt taaccgcgct ggcactgttg ttctccaact
     5101 cctatggcgg catggttact cactatggcg gcgcggcagg tccggtcatc tttggcgtgg
     5161 gttacaacga tattaaatcc tggtggttgg tcggtgcggt actgacgata ttaaccttct
     5221 tggtgcatat caccctcggc gtgtggtggt ggaatatgct gatcggctgg aacatgctgt
     5281 aaatatactc gtcatacttc aagttgcatg tgctgcgtct gcgttcgctc accccagtca
     5341 cttact
//
LOCUS       DAZPAB010000232         5340 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZPAB010000232 DAZPAB010000000
VERSION     DAZPAB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.233"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACHUPG_003691"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="ACHUPG_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR0863112.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="ACHUPG_003692"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="ACHUPG_003692"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR0863113.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="yfiH"
                     /locus_tag="ACHUPG_003693"
     CDS             1884..2615
                     /gene="yfiH"
                     /locus_tag="ACHUPG_003693"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR0863114.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="ACHUPG_003694"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="ACHUPG_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR0863115.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1290 c         1458 g         1282 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcgggg
//
LOCUS       DAZPAB010000233         5284 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZPAB010000233 DAZPAB010000000
VERSION     DAZPAB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.234"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(140..901)
                     /gene="kduD"
                     /locus_tag="ACHUPG_003695"
     CDS             complement(140..901)
                     /gene="kduD"
                     /locus_tag="ACHUPG_003695"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR0863116.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(931..1767)
                     /gene="kduI"
                     /locus_tag="ACHUPG_003696"
     CDS             complement(931..1767)
                     /gene="kduI"
                     /locus_tag="ACHUPG_003696"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR0863117.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(2054..3235)
                     /locus_tag="ACHUPG_003697"
     CDS             complement(2054..3235)
                     /locus_tag="ACHUPG_003697"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR0863118.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3490..4719
                     /locus_tag="ACHUPG_003698"
                     /pseudo
     CDS             3490..4719
                     /locus_tag="ACHUPG_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            5146..>5284
                     /locus_tag="ACHUPG_003699"
     CDS             5146..>5284
                     /locus_tag="ACHUPG_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0863119.1"
                     /translation="MQLYTDREVLNMGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCA
                     NS"
BASE COUNT         1436 a         1297 c         1118 g         1433 t
ORIGIN      
        1 tttaaagcag ataaaattca taaagttcat taattgataa ttgatatgga ttatttcata
       61 accatgatat ggattatgat gatctagaga tataaaaaac cctgccatgc ggcagggtca
      121 taaaagtaag aggaatgaat taacgcgcca gccaaccgcc atccacagca atggtataac
      181 catttacata atctgaagcg ctggaggcaa gaaacactac cggccccatc aggtcactcg
      241 gcagtcccca acggccagct ggaatgcgat cgagaatttc cgcgctacgt tgttcatctg
      301 cccgcagttg ttgggtattg ttggtcgcca tgtaacccgg agctatcgca ttaacattaa
      361 tgttgtgttt agcccattcg tttgccatca atcgcgtcac acccatcacg ccgctttttg
      421 atgcggtata agaaggcaca cggatcccgc cctggaagga gagcattgac gcgatattga
      481 taatcttgcc gccattgcct tgcgcgataa agtgtttcgc cgctgcctga gacatgaaga
      541 atacgctctt gatattcagg ttcatgacat cgtcccagtc tttttcgctg aactcgagag
      601 cgtcttcgcg gcgaatcaat ccggcgttat tcaccaggat atcaatatga ccaaattcag
      661 ctaccgcgcg atccagcagt ccaggaatgc catcaatctt tcgcagatcg gcggtcaggc
      721 ttaaaaaacg acgccccagc gccgtgacct gctcgatggt ttcagtcggt tcaacgatgt
      781 taatgccaac aatgtcacag cccgcttgcg ccagccccaa cgccatcccc tggcccagcc
      841 cagtatcaca accagtgacg accgcaactt taccttggag agaaaatgca cttaaaatca
      901 taacaacacc ttattcgttt atgcccacaa ctagcgcaaa tctttaacgc ccacatggtc
      961 catatcatta aagacctggt tttcaccgac catgccccag ataaaggtat aagctttggt
     1021 tccgacgccg gaatggatcg accagctcgg ggagatcacc gcctgctcgt tatgcatcac
     1081 aatgtgacgc gtttcttgcg gctgccccat catgtggaaa acgcaggcgt catcatccat
     1141 attgaaatag aaataaactt ccatccggcg ctcgtgggtg tgacacggca tggtgttcca
     1201 caagttacct ggagccagct ccgtcagccc catactcaat tgacaggttt ccagtacatc
     1261 cgggacaaaa tatttgttaa tcgtgcgacg gttactggtg aggttatcgc ctaacgtgac
     1321 tggagatact tcgtccggtg tgactttttt ggtgggatac gtcgtatgcg cgggtgcgca
     1381 attgtaataa aacttcgccg gagtggcggt atcaatactg gcaaagacaa cttcttttgc
     1441 acctttacca acatacaggg cgtcgcggtg accgatttca tagcattgac catcgacagt
     1501 aatcgtaccg gcaccgccaa tattgataac acctaactcg cgacgttcaa ggaaatagct
     1561 tacgccgagt tgtttaccaa cttccccgcc aacggaaacc gttttagtta tcggcataat
     1621 gccgccaaca ataattcggt caatgtggct gtaaaccatg gtgtactcat cggcgacaaa
     1681 taccttttca accaaaaatt cattgcgcag cccttgggta tccagcgttt ttgcgtgcgc
     1741 accgtggatg ctctgtctta cgtccacatc aacctccgat aatcagtgaa gcgtgaaaat
     1801 agaaaacgat agtgaaacac tgtttcgttt tctctataaa gggatgatat gcccatgggc
     1861 tagggttttc aattaaaaat aaaacaatgt tttattttta atttaaggat taaaaaaagt
     1921 gcagtgtcga tcacgaatat gtcatgagaa tggaaaaatg agggagccca gaaaatgtat
     1981 gtcgacttgc cagccatcat tattcaacag attaatgacg gcagatattt atcggtaagg
     2041 aggaaatgca gagctactcg tcacgttcaa tagtcaatgc cacgccctgg cccccgccga
     2101 tacaaagcgt tgccagccct ttgcgggcat tacgtttcac catttcatga accagagaaa
     2161 ccaggattcg gcaaccggaa gcgcctatcg ggtgaccgag tgcgatcgcg ccaccattga
     2221 cattgacccg acgctcatcc cactcaagca tcttgccaac cgaaagcgcc tgtgcagcaa
     2281 acgcttcatt agcctcgata agatcgacat cagccaactg ccagcctaca cgctccaggc
     2341 aacggcgggt cgcatacacc ggcgcaattc ccatcaatgc cggatctaca ccaacgctgg
     2401 caaatgcgcg gatgcgggcc agcacgggta aattcaacgc tcgtgctttg gcttcgctca
     2461 tcatcattac cgcagctgcg ccatcgttta tggatgatgc attccccgcg gtcacagaac
     2521 cgagactatc aaatgaagga tttaaacggg ctaaaccttc tgcactggcg tcagtgcgtg
     2581 gctgttcatc ggtatcaaca accaacgtct gaccgttact ttggatcatt accgggacga
     2641 tctcatcttt aaatcgtccg gcgtcaatcg ccgctcgcgc tttttgttgc gagctaagtg
     2701 cgtaagcatc ctgcaactga cggctgatgc catattcgcg agccagattt tcggcggtga
     2761 caccaatatg ataatcattg aaggcatccc acaacccatc atgcaccaga ctgtcaacca
     2821 actggctatt gccaagctgt tcaccggtgc ggctatcagt cagaacatgt ggtgcgcggc
     2881 tcatgttttc ctggccacct gcgatgacaa tatcagcctc gccacactgt atcgcctgag
     2941 tagccagatg cagtgcttta agaccggaac cgcaaacgtc attaatcgtg attgcagaaa
     3001 cgctgttagg aagaccacct ttaatagccg attgccttgc cggattctgc cctgcccctg
     3061 cagtcaacac ctgaccaaga attacttcat ccaccgcata tgcaggaacg ccggtacgtt
     3121 ctattaacgc tttcacgacc agactaccaa gttccacggc ggaatgaccc gctaacgcac
     3181 cacgaaagca gccgataggt gtccgtaacg ccccgacaat cacaacgtct ttcatcacaa
     3241 cctcgcacca attaacagtg caatagtaaa tgattgttaa ttacagttat cttaattgtt
     3301 taaaaaaagt gatttttatc acaaaggaaa tatgcctgag cagtagtcag agtcataact
     3361 ggcacgtaag gtttgcaacc actaacccac caatagatgg gtagataggg cgttaatctc
     3421 ccatacttaa cttggtttat ggtaaattgc cctccatttt gtttaatttg tagatgatac
     3481 gttcagataa tgtctaatat ttggtctaaa gaagaaactc tgtgaagttt cgcgctctat
     3541 ggcacagccg ttgatgcagg cacgctattc cttcctattc agttaggttc ggcaggggct
     3601 gtggtcctgt ttattactgc tctggtcgcc tggcctttaa catattggcc acataaagcc
     3661 ttatgccagt tcatcctctc atcaaaaaca tcagcaggtg aagggataac gggcgcggta
     3721 acacactact atggcaagaa gattggtaat ctgattacca cgctgtactt catcgccttt
     3781 tttgtcgtcg tgttgatata tgcagtggca attaccaact cacttacgga acagctggca
     3841 aagcatatgg ttattgatct tcgcatccgt atgttggtga gtctgggtgt tgtattaatt
     3901 ctgaatctca tttttctgat gggacgtcat gccactattc gggtaatggg atttttggta
     3961 ttcccattga ttgcctattt cttatttctt tccatttacc ttgtcggtag ttggcaacct
     4021 gatctattaa caacccaggt agagttcaat cagaataccc ttcaccagat atggatatcg
     4081 attcccgtga tggttttcgc ctttagccat acgcccatta tttctacgtt tgccatagac
     4141 agacgtgaaa aatatggcga acacgctatg gataaatgca aaaaaattat gaaagtcgct
     4201 tatctcatca tctgcataag tgtactgttc tttgtcttta gctgcctgct ttctattcca
     4261 tcttcgtata ttgaagcggc taaagaagaa ggggtcacca ttttatcggc gctttctatg
     4321 ctgccgaacg ccccagcatg gttgtcaatt tccgggatta ttgtcgcagt agttgcgatg
     4381 tcgaaatcat tcctgggtac gtactttggc gttattgaag gtgccacaga ggtcgtcaaa
     4441 acaacactac agcaggttgg tgtaaagaaa agtcgtgcat ttaaccacgc actatcaatt
     4501 atgttggtat cgctgattac cttcattgtt tgttgcatta acccgaacgc gatttcgatg
     4561 atttacgcga tcagcggccc gctcattgcc atgatacttt tcatcatgcc tacgctgtca
     4621 acgtatctca tcccggcgct taaaccctgg cgttccatcg gaaatctgat tacgctgatc
     4681 gtgggtatcc tgtgcgtatc ggtaatgttc tttagctaat atccgttgtg gatggttcaa
     4741 aattgctaca attttgaacc attccatttt tcataatatt catttgttat tattccgccc
     4801 tatcacttct ctcgtcatat cctagaatta tcagttagtt aaacacacaa agagatctga
     4861 acatcttaaa taagactatt taagatgcat aacttagatt cgcaagatat cttctggtca
     4921 ttcaaaaaca atttgcgata tagatattct cataccatga gatagttatc tggaccacgt
     4981 tcgaccatgc aagattaaat cagtgaaaca taatcatatt taataacgag aaatgcattt
     5041 ttaaatatct taatcagaat tatagcaaat acagattaat cgcaattttt cccagcaaaa
     5101 ttacaaaatc aaggtgtatt gacatcatca atcaccaatg aaattatgca attatatacg
     5161 gatagggagg ttcttaacat ggggctttgt agtcgttata aaagtcttac atgcaatagt
     5221 tgctcaatgc attgccaaat aatgccagaa gagtcaccgc gtttgcagta ctgcgcaaac
     5281 tcgt
//
LOCUS       DAZPAB010000234         5275 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZPAB010000234 DAZPAB010000000
VERSION     DAZPAB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.235"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHUPG_003700"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHUPG_003700"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR0863120.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHUPG_003701"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHUPG_003701"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR0863121.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3324)
                     /locus_tag="ACHUPG_003702"
                     /pseudo
     CDS             complement(2698..3324)
                     /locus_tag="ACHUPG_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3454..5214
                     /gene="ycaO"
                     /locus_tag="ACHUPG_003703"
     CDS             3454..5214
                     /gene="ycaO"
                     /locus_tag="ACHUPG_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR0863122.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1345 a         1358 c         1263 g         1309 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc cagctgctcg aaataatccg gaaatgtttt ggccgtgcat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg aggagtgata
      241 cgaatgtaat cgtgcccctc ttccacttcc gcgccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcaggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc caggaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gctctttcat acgcggctca ccggtcagca caatatcatt gctatccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttaaccc taacgctgtt aatgcattca gcatatggcg cacgtcgtcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgcaag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggca ttataaatag acgcgcgcat
     1501 tccgccgacc acacggtgtc ctttcagtgc atgaaggcct gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaattaca ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtctcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc cgcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgcgttca tatgaatgat ttcactacaa cgaatgcagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatcac attaccaggc
     3061 ggtataacaa cgccagagtg ataaagacga taagtaccgg caccatcggc acatcatcat
     3121 aaaacgccac atctcccgcc gctgaaccca gcgtgagaat gattaacact tcaaacagcg
     3181 acatctgtcg cacaccgcgt cttccggtca ttttgaggaa caaaaaacca gtacaaaggt
     3241 ataaagacta cgtagtgcaa cttcgccaag gaaatcaaaa ggcactttat caaatgccat
     3301 acggtggaga tcgaacgcct tcatttttat gctcttaacg tggtttatcc gctaaaagca
     3361 tagtcaacag ttcatcggca tggcggcggt gatagcgcct ttgagataaa acccgtatta
     3421 ttgcgcgctt tccgtacgac taaagtgatt ttcatgacgc aaacatttat ccccggcaaa
     3481 gatgccgctc tggaagattc catcgctcgc ttccagcaaa aactttcaga cctcggcttt
     3541 cagattgaag aggcctcctg gctgaatccc gtgcctaacg tctggtctgt acatattcgc
     3601 gacaaagagt gcgcactgtg ttttaccaac ggtaaaggcg caaccaagaa agcggcgctg
     3661 gcttctgcac tcggtgaata tttcgagcgt ctctcaacca actacttttt tgctgatttc
     3721 tggctgggcg aaaccatcgc caacggtcca tttgtgcatt atcccaacga aaaatggttc
     3781 ccactgagcg aaaatgacga tgtgccagaa gggctgctcg atgaccgtct gcgcgcattt
     3841 tacgatccgg agaatgaatt gaccggcagt atgctgattg acctacaatc cggtaacgaa
     3901 gatcgtggta tttgcggtct gccgtttacg cgccagtccg acaatcagac cgtttatatt
     3961 ccgatgaata tcattggtaa cctgtacgtt tctaacggta tgtccgctgg caatacccgc
     4021 aacgaagcac gcgttcaggg gttgtccgaa gttttcgaac gctacgtgaa aaaccgcatt
     4081 attgctgaaa gcatcagctt gccggaaatc ccggcagacg tgctggcgcg ttacccagcg
     4141 gtggttgaag cgatcgaaac actggaaaca gaaggtttcc cgatcttcgc atatgatggt
     4201 tcccttggcg gccagtatcc ggtgatttgc gtggtactgt ttaatcctgc aaacggcacc
     4261 tgctttgcct ctttcggtgc gcatcctgat tttggcgtag cactggaacg taccgtgacc
     4321 gagctgctgc aaggtcgtgg cctgaaagat ttggatgtgt ttactccgcc aaccttcgat
     4381 gatgaagaag tcgctgaaca taccaacctc gaaacgcact ttatcgattc cagcggttta
     4441 atctcctggg acctgttcaa gcaggatgcc gattatccgt ttgtggactg gaatttctcc
     4501 ggcaccacgg aagaagagtt tgctacgctg atggctatct tcaacaaaga agataaagaa
     4561 gtttatattg ccgattacga gcatctgggc gtttatgctt gccgtattat cgtgcctggc
     4621 atgtccgata tttatccggc tgaagatctg tggctcgcga ataacagtat gggcagccat
     4681 ttacgtgaaa cgattctttc gctaccaggc agcgagtggg aaaaagaaga ttacctgaac
     4741 ctcatcgagc aactggatga agaaggtttt gatgacttta cccgcgtgcg tgagctgttg
     4801 ggtctggcga ccgggtcgga taacggttgg tacaccctgc gtatcggtga attaaaagcc
     4861 atgctggcgc tggctggtgg cgatctggaa caggctctgg tctggaccga atggacgatg
     4921 gagtttaact catcagtatt tagtccggaa cgcgccaact attatcgctg cctgcaaacg
     4981 ttgttattac tggcacagga agaagatcgc cagccgctgc aatatctgaa tgcgtttgtt
     5041 cgcatgtacg gcgcagatgc cgtagaagcc gccagtgcgg caatgagcgg cgaagcggcg
     5101 ttttacggcc tgcaaccggt agatagcgat ctgcacgcgt ttgctgcaca tcagtcgttg
     5161 ttgaaggcct acgaaaagct gcagcgcgcc aaagcagcat tctgggcaaa ataaaatcaa
     5221 atagcctacg caatgtaggc ttaatgatta gtctgagtta tattacgggg cgttt
//
LOCUS       DAZPAB010000235         5241 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZPAB010000235 DAZPAB010000000
VERSION     DAZPAB010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.236"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            155..586
                     /gene="osmC"
                     /locus_tag="ACHUPG_003704"
     CDS             155..586
                     /gene="osmC"
                     /locus_tag="ACHUPG_003704"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR0863123.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(642..1568)
                     /locus_tag="ACHUPG_003705"
                     /pseudo
     CDS             complement(642..1568)
                     /locus_tag="ACHUPG_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
     gene            complement(1561..2535)
                     /locus_tag="ACHUPG_003706"
     CDS             complement(1561..2535)
                     /locus_tag="ACHUPG_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0863124.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            complement(2532..3428)
                     /gene="ddpC"
                     /locus_tag="ACHUPG_003707"
     CDS             complement(2532..3428)
                     /gene="ddpC"
                     /locus_tag="ACHUPG_003707"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFR0863125.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(3442..3774)
                     /gene="ddpX"
                     /locus_tag="ACHUPG_003708"
                     /pseudo
     CDS             complement(3442..3774)
                     /gene="ddpX"
                     /locus_tag="ACHUPG_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            complement(4032..4649)
                     /locus_tag="ACHUPG_003709"
                     /pseudo
     CDS             complement(4032..4649)
                     /locus_tag="ACHUPG_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(4574..>5241)
                     /gene="dosC"
                     /locus_tag="ACHUPG_003710"
     CDS             complement(4574..>5241)
                     /gene="dosC"
                     /locus_tag="ACHUPG_003710"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFR0863126.1"
                     /translation="SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDS
                     DLGSSLPLSQADFGLWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNR
                     SLRVKFLLQIRNTVSQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHA
                     NRTGTPLSVLIIDVDKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKL
                     AVS"
BASE COUNT         1332 a         1360 c         1298 g         1251 t
ORIGIN      
        1 cttgagatat atattcccgg ttatctattg tgggaattta atttaagtgc agaagtaata
       61 ttttcgccgt attttattcg gaatatcctg cttatcctcg tgctgtttct cacgtagtct
      121 ataatttcct ttttaagccc acaggagagc aacaatgaca atccataaga aaggtcaggc
      181 acactgggaa ggcgatatca aacgcgggaa gggaacagta tccaccgaga gcggcgtgct
      241 gaaccaacag ccgtatggat ttaacacgcg ttttgaaggc gaaaaaggaa ccaaccctga
      301 agaactgatt ggcgcagcgc atgccgcatg tttctcaatg gcgctttcat taatgctggg
      361 ggaagcggga ttcacgccaa catcaattga taccaccgcc gatgtgtcgc tggataaagt
      421 ggatgccggt tttgcgatta cgaaaatcgc actgaagagt gaagttgcgg tgccgggtat
      481 tgatgcctct acctttgacg gcatcatcca gaaagcaaaa gcaggatgcc cggtctctca
      541 ggtactgaaa gcggaaatta cgctggatta ccagttgaaa tcgtaaagca ttgccggatg
      601 acgcgtcagg cgcgtgaatg cctgatgcgt tgttagcatc tctacagcgc ccgccagcac
      661 cggaggtcac gtccgtcctc ccttattgct aatgattgcc ggacttcaca tccgtgggtt
      721 gccagagggc aacgttcgta gaaaaaacag ccttgcggca acgtgcggtt tcccggcaga
      781 tccgttttac gtaatgctca ttcttcctcc agcggtttat caatggcggg gagggaatcc
      841 agcaataatc gggtgtatgg atgtgccgaa gcagtcaaca cctgttgcgc gtcgcccagt
      901 tctacaatct gcccgagata catcaccgcc acccgatcgc tcatatgacg tatcaccgag
      961 acattgtgtg aaatcagcac ataagtcagc ccgtgatttt cctgtagcgt taccagcaaa
     1021 ttgaggatct gcgcctgcac ggagatatcc agcgccgagg ttggctcatc aagcacaatc
     1081 acgtcaggct gcgaagagag tgctctggca atggcgatgc gttgccgctg cccgccggag
     1141 aacgcatgag gcaggcggtc gagatactcc ggacgaatac ccacctgcac agccaactcc
     1201 tctgccaacg ctcgccgctg ttgttcacta ctacgcttag ctatccagag cgattctgtg
     1261 atgatgcgcc acaccggtaa gcgcggatta agcgaagaga gcgggtcctg aaacaccatc
     1321 tgcataatgc gttgtgagcc tgaacggatg tactgcccgt ggctcggttg cagcatgccc
     1381 attaaaagct gtgcgagggt gcttttgccg cagcctgact cgccgacgat ccctaaggtt
     1441 tcaccacggc ggatctgtaa atcaatacca ttaatggcat gaacatgttc cgtagatttg
     1501 ccaagccagt ttttacgggc cggaaaattg atatggacgt cgcgtaacgt taataacgtg
     1561 tcagacactt atgacctcct gctgcggata ccaacaggtg cagtgctggt tgttgtcacc
     1621 acacgctgtc agcgcacact gtgcacctgc cgcatagcaa cgatcgcgaa aagcgcagcc
     1681 gtcaggcaaa tgggtgaggt ttggtaccgt ccctggaatg gcgggtaatg gctggcgtgg
     1741 tactccatgt tctggtgcgc attgcagcaa accaatggta tacggatgcc ggggatgatg
     1801 gataacgtct gccgtcccgc cgctttcaat tacgcttccg gcatacatca cgtaaacgct
     1861 atcgcacagt tgcgacacca cggccatatc atggctgatg aacagtaccg cagttccact
     1921 ggcgcgtgct ttatgtttaa gcaaacgcag tacctgcaac tgtactgtga cgtccagcgc
     1981 cgtcgtcggt tcgccggcaa taatcaattg cggctcgcag gagaatgccg gcgcaatcat
     2041 tacccgctgg cgcataccac ctgaaagctc aaacggatag cgcgacataa cttccacggc
     2101 atccgggatt tgcatctctt ccagcagtgc aatcgcttta gctctggctt cccgacgact
     2161 tattggttga tgatggcgga ttacgtccat catctgaaga cctattcgac gtgtcggatt
     2221 aagggcggtc atcggttcct gaaagatcat cgccactcgt gcgccgcgcc actgacgaag
     2281 ctgtttttcc cgagcgttaa gaacatcttc tcccagcagt gaaatctgcc cccgatgtac
     2341 gcaataactg cccgtcggta gcagacgcat aatcagcatt gcggtgactg atttacctga
     2401 gccggattct cccaccagac cgacaatttc gccgcggtta atctgcaagg acacattgtt
     2461 gagcgcgtga acatcaccgt taaaaccggg gaaactcaaa tgcagctgtt gaatgtccag
     2521 aacgggttgg gtcatgactg ctttcctcct gctttcgggt caagcagatc gcgaatacca
     2581 tcaccaaaga gattaaaccc gacggaggta agcaaaatcg ctgcccccgg aaatgcgcaa
     2641 taccaccact gatcgagtac atagttgcga ccattcgcta ccatcgcgcc ccattcagca
     2701 ctcggttgtt gagcacccag gccaataaat cccaacgtgg cggccattaa aatcgcgcta
     2761 ccgatatcca gcgatgcctg cacgatcagc ggcggtaggg agttacgtaa aatatgccag
     2821 ttgatcagat gccaacgaga cgcgccaaag gttttcgccg cctgaacata ggtatactgg
     2881 cgtacaacta atgcttgccc ccgcgccaag cgcacataaa agggaattcg cacaatagca
     2941 atcgccagca tggcgttaaa taaactcggc ccgagagcgg cagccagtgc cattgtcagt
     3001 accaacgaag gaatcgacag cataatgtcc atgatgcgca tgatgatggc gtctgcgcgt
     3061 ccgccaagca caccggatag acatccgagt agcgaaccaa tcatacccgc aatggcgacc
     3121 accactaatc cggcgagaat tgattgctga ctgccgacca gtacgcggct aaacagatcg
     3181 cgtcccactt catcggtgcc aaaccagtgc gccgcagaag gcggcaaaag gcgggcggtt
     3241 aaatcaatgg cgttgggatc atgcgtcgcc agccacggtg aaaaaatcat catcagtcgc
     3301 attaatacaa tgatgactgc gccggtcacg gtgagcggac tgcctttcag catccatacc
     3361 agttttgcac cgttaaatcg cgtttgtttt tgtgggcgta cggcggacgt ttcctcgctt
     3421 agcatcattc accacctcca cgtcatcacg aagcgtcagg tcgagcgccg tgccacggct
     3481 gtgattagaa ccgaccgtca catcaacaac atattgcggg tctgggcagg cttgccacaa
     3541 catcgcctgt gcttgttgtg ggcgatacgc atcgtaaatc accagttgta agcctgacag
     3601 ctgggcgata ctgatacttt tcgccagcgc ggtaatcgca tctttatgta gcagacaacg
     3661 cgcttgctga taaatagctt tacctgtgat gttatcaacg caggcatatt tcaattcgat
     3721 ctccagatca gggaagatca cggctaaatc aaccagttcg gtggtatccg acataaacgg
     3781 ttcctgtata agacaaaaat tgctgcgctt tccgcttatg cagatctcat gccatgccgg
     3841 gataagcgcc agaatgctgg cttaaagtta tattttttaa cttttgatca acatttgtgc
     3901 agcgtagtgc agttttggtg caagagggaa agttaaggaa ggaatctccc ggaatcgtag
     3961 ctgaaatcac agtatttaag tgacagtgtc accttaaagg aaaacccgcg agtgcgggcg
     4021 agaggaattt gtcagatttt cagcggtaac acgctgctca tccagcctgg aatttcttcg
     4081 gcgggaaggg ggcgggaaaa gaaatatccc tgaataacgc gacagtggat cttacgtagc
     4141 atctcaaatt gctctttggt ttcgacccct tccgccacga cggttaaatt gaggctttgc
     4201 ccaatgctgg taatggcttc aagtaaggcc aggatgcgtt tttccgtcag acaacgatcg
     4261 acaaagcttt tgtcaatttt gatttccgtt accggaagac tgactaagcg ggataatccg
     4321 gaaaagcccg tgccaaaatc atctaccgat aaacccacgc ccatatcacg caggatctga
     4381 atgcgcttaa agatttcggt atcgtgttcc atcatcatgc tttccgtgat ttccaccgtc
     4441 agctggtggc cgtcaatacc ccaggcttgc attgcatcag acacctgatt aggcagctga
     4501 ttactgcgaa agtgcagcgc cgacaagttc acggataacg ccgggatatg aatattctgg
     4561 ctacgccatt ctgctaactg acggcaagct tccgcgatga cccagcgccc aatattttcg
     4621 atttcaccaa tctcttctgc gagaggaatg agactttacg cagaatttca tcgccagtgt
     4681 tatggcccca cgtatcgttg atctctttga atttatcaac gtcaataatc agcactgaca
     4741 gcggtgtacc ggtccggttg gcatgggcaa tttcgcgttt gaagatagtc ggcaggaaac
     4801 ggcggttaag taatttcgtc agtacatcca taccgacttc gtggcgcgat acttcttcaa
     4861 acaattcacg cagcaaggta ataatctgcg atacggtatt tcttatctgt aatagaaatt
     4921 tcacccgcaa acttctgtta ttcaaaatcc tggtgttacg catggtttga ttgaaaatac
     4981 cgtcgaaatc ctgaatcaga cgggagatat ggcccacttc ggcaatgccg ctaaaataat
     5041 gtcgaccttt atggttaaac cacaggccaa aatcagcctg gcttaaaggc aaactgctgc
     5101 ctaaatcaga atccagcagg actttataga taatatctat ttcccatgaa agtattgagg
     5161 ctatttgccg ttctttttct tcttcggcgt tttccagtaa cgagaagata cgatagtttt
     5221 catcttcctt tgaggcacta c
//
LOCUS       DAZPAB010000236         5237 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZPAB010000236 DAZPAB010000000
VERSION     DAZPAB010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.237"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..171
                     /locus_tag="ACHUPG_003711"
     CDS             <1..171
                     /locus_tag="ACHUPG_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0863127.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..1628
                     /gene="celF"
                     /locus_tag="ACHUPG_003712"
                     /gene_synonym="chbF"
     CDS             276..1628
                     /gene="celF"
                     /locus_tag="ACHUPG_003712"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR0863128.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1641..2399
                     /gene="chbG"
                     /locus_tag="ACHUPG_003713"
     CDS             1641..2399
                     /gene="chbG"
                     /locus_tag="ACHUPG_003713"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR0863129.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2446..4707)
                     /gene="katE"
                     /locus_tag="ACHUPG_003714"
     CDS             complement(2446..4707)
                     /gene="katE"
                     /locus_tag="ACHUPG_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR0863130.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4890..5153
                     /gene="cedA"
                     /locus_tag="ACHUPG_003715"
     CDS             4890..5153
                     /gene="cedA"
                     /locus_tag="ACHUPG_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR0863131.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT         1285 a         1177 c         1385 g         1390 t
ORIGIN      
        1 atccgtatta attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg
      421 atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt
      481 ataaaacgct ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc
      541 gcgttggcca attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc
      601 ttggtcagga aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga
      661 tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca
      721 ctaacccggc gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta
      781 tcggcgtgtg taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga
      841 aagacagcga tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaagg
      901 atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc
      961 agttaaaagt atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc
     1021 gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc
     1081 tggctattga aatgggcgaa tactacaaag gcggcgcacg agcgcaggta gtacagaaag
     1141 tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac
     1201 tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatct
     1261 acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata
     1321 ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac
     1381 atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct
     1441 tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc
     1501 taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga
     1561 ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag
     1621 cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct
     1681 taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga
     1741 cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac
     1801 cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg acagctatgc
     1861 cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg
     1921 ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc
     1981 tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga
     2041 ttttcccgat tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc
     2101 agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta
     2161 gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa
     2221 gccatcgggg tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta
     2281 tccgtcagag tgcttactgt ttcccacggt taacggagct ggaagtgctg acatcagcgt
     2341 cgttgaaata tgcaattgct gagcgtggtt atcggttggg tagttatctt gatgtgtaag
     2401 taaggtggta aggtcactcc ccaggggacg tgaccttatc catatttacg ccggaatttt
     2461 gtcaatctta ggaatgcgtg accacacgcg gtgttctgcc atcagcgtta acagttcatc
     2521 cataaaacta ccgtcggtgc tgtcagcttc cacaatccct tcttcaccct ggtcagctac
     2581 cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt
     2641 gtaggcttcc atcagatagt agttggcatc gccgttgtca gcgatatccg cgatattgcc
     2701 gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat
     2761 aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga gcagtttggc
     2821 atgaacgcct ttggccttca tggccttgag aatggccaga aggtctgccg atctcacttc
     2881 atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa
     2941 acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga tattcagctg
     3001 gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag tgagatcaat
     3061 atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt tgcttaactc
     3121 aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta gccagaacag
     3181 acgcggatgg gaataatatt cgccaaacga tgggctgcgc tcgcgaactt tattgccttc
     3241 cacgcgctct tggtatgatt caaaaccgcc gcgtttcggc cccggtggtg tttcgcgcgg
     3301 ccagttatcg ttgatcgagt tcggttcata attcgccggg ttagtgtcga tccccatacg
     3361 atgcatgccg tcacgctgga aattatggta agggcaggtc gggcggttaa tcggaatctc
     3421 atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga acaaacgtcc
     3481 ctgcaacagg ggatcgttgg tgaagtccag accaggaaca atatgacctg gatggaatgc
     3541 tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca ttttgccgac
     3601 acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa gatcgaagtc
     3661 gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt ccggaaaatc
     3721 gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac gtccggtgag
     3781 tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc agtggaaacg
     3841 tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac cgaagccttc
     3901 catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca cgttgtgcag
     3961 agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt gcccttgtgg
     4021 aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc
     4081 ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct cttcagtata
     4141 gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag cgccaccctg
     4201 aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga ggaaatccgc
     4261 tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg ctgagccgcg
     4321 tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag
     4381 aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt tttgatcatc
     4441 ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt tgcgtacgtc
     4501 ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc caggggcggt
     4561 aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt cctcaggtgc
     4621 cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg
     4681 tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt actgaaaggg
     4741 ccgctgtttt ggtcaaaaaa ctctctaact atagaccacc agccagatcc cgcttcggag
     4801 actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg
     4861 gctaaactac agtttttcta atttttaata tgcgtttagt gaagccagta atgaagaaac
     4921 cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa cccgcgccgc
     4981 cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg
     5041 ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat
     5101 ctgcccaacg ttgggcaaat cagatccgcc aggaagggga agtgactgag taaggattac
     5161 atacctcccg gttttgccgg gagttacagg acctgaaatt ctgcatttcg ttgctacatt
     5221 cattcaaacc acctgta
//
LOCUS       DAZPAB010000237         5163 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZPAB010000237 DAZPAB010000000
VERSION     DAZPAB010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.238"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..220
                     /locus_tag="ACHUPG_003716"
     CDS             <1..220
                     /locus_tag="ACHUPG_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0863132.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(392..721)
                     /locus_tag="ACHUPG_003717"
     CDS             complement(392..721)
                     /locus_tag="ACHUPG_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR0863133.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(726..911)
                     /locus_tag="ACHUPG_003718"
     CDS             complement(726..911)
                     /locus_tag="ACHUPG_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR0863134.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(908..3547)
                     /locus_tag="ACHUPG_003719"
     CDS             complement(908..3547)
                     /locus_tag="ACHUPG_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR0863135.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3755..4744
                     /gene="ldhA"
                     /locus_tag="ACHUPG_003720"
     CDS             3755..4744
                     /gene="ldhA"
                     /locus_tag="ACHUPG_003720"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR0863136.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4855..>5163
                     /gene="hslJ"
                     /locus_tag="ACHUPG_003721"
     CDS             4855..>5163
                     /gene="hslJ"
                     /locus_tag="ACHUPG_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR0863137.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     N"
BASE COUNT         1307 a         1323 c         1203 g         1330 t
ORIGIN      
        1 tttgtaccga atgtttgccg ataaaggttt ggtagtcgcg caatatattc gtaaccgtcg
       61 tctcgatttt tgtgcagatg cgattcgcca tgccgcagat gatgaaaaac tggcaggcat
      121 cggctttcat tggggatttt ctgaccagag tcatttttca acggtattta agcaacgctt
      181 tgggatgacg ccaggcgagt atcgacgtaa attccgctaa ctaattggcg ttgcatgtac
      241 tgcaacgcca attagttaaa acagatattt gactatgcat ttttcagggc gaaaggcaat
      301 ttgacaggag tttttgaata ttcaccttgt ttcaggaaaa tagatcaatc gctttctcct
      361 gtagcgttat cccgatattg cggttttgct attaaaactt tcgcacccat ttaccgttta
      421 tcccttgtac atattccccc ggtttggccc gagctacgag cttttgcccg gcgagtttcg
      481 cgatatcatc tacagacaca ttattttgct ttgcccgttg ttggtaactc gctttacgtt
      541 cggcattaat ttcggttact aatttctctg tttcagcatc cgttttcagc gcaaccagat
      601 aaccataaaa tgtttcacct acccgcccct gagttctggc ttcatccaga gtcaatgcca
      661 ttacattgct gcttaccagc ccaacaagaa acgcacaaag aagtaatgtt ttcttcatca
      721 tcgcctcaga aaagatcgct acgagtttca agcagctctt cgacatcttt gtctgccttg
      781 atgatgatct catgctcaat tttaacgttc atattgatag tgatcggttc cttaggtgca
      841 gcgacttcaa tgcgaggcgt acagccaacc agcataaaag atgacgtcaa cgcagccagt
      901 aaaattttca ttgtttttcc tcacactctt ttccttgcgg acagtcattt cccggcagac
      961 gtgcgttctg ctccaaccat gcctggagat tatcgccaaa gcgtaaactg cgccacagcg
     1021 taaaaatatt ctcttcatga tggtaattaa ggtttaccgt accgctttta ccatcaacgc
     1081 gactggtacc tgtaatgttg gcctgcatgg ttaataaacc gagattatct aagttaattt
     1141 ttgtcgatga ccggctaatt tccatatagc gcaaccagtt aattgctgaa cccgcagtca
     1201 tattgtcttt caccaccgca tctgcggtat ctttatccag ccgcaaggtc attagcccgc
     1261 tatttgccag ccaaccatct ttcactatcc atttttcgtt gtttagccat aacggcaatg
     1321 caccactaaa tgccccggac atggcgaatt gtttcggatt gacggcgcta accagttcgc
     1381 tggatgagag attattcagc cgcaacagcg ccgggtcatg ttgcggcata cgtaattgtt
     1441 tcatcagcac gttaccgcct aacacatcga cgctaacatc agtcaacagc aagggttctt
     1501 cttcagtcca cggataacgc ccttgcaaat cagccgtaat atttttcgct gtcaccagat
     1561 taatcacttc ggcaattcgc aacgtaacgg ggccgcgagt ccccagatgc caggctccat
     1621 cggcaaaacg gaaaggcagg acaaaatcga caccgttaac ctgattatct ggcatccagg
     1681 cactaccgcc tttcaacacg ccgtgccctc ccgcgcggaa tccttgttca ggcgcagcag
     1741 aaaatgcaac ctgagcatac agttcaccat cgcgtaagtt catcttccag tcgggtggca
     1801 ccagcggctg gaatacagtc agtgattgtt ttggccacca ggcgttaccg cgcagacgaa
     1861 taccgtccca gcgaccattt acccgaaccg ggccaatctc accagcatgt aaatcgcctt
     1921 taaagaggaa ataggtcgga ttgcgcccat cgacgctaaa ttttaaggtt gatggcggta
     1981 acacactgcc gccagtgaac agcgtttgcc cggcgtccag tgacagcgcg ccgctgaagg
     2041 agggatgctg tgcgtcacgt acccagacga cgggcttgtc gagaattaat cgcggctttt
     2101 ctaccgtcat cgtaccgtat tgtaactggt cgaaaccggt ggagagatcg gtcagcgtaa
     2161 tcgtgctgtc atgccactca ccttttcctg cgacatccca ggtggcattc atcggtgtaa
     2221 aactcccttt tccccagtag cgccactgcc agcggccagc gtcagggaga aaatcattcg
     2281 ccagcccatc catatgcagc acgaaatcgc ccagttcatt ttcatgcgcc tgcaaaatgg
     2341 cctgcaaacg tccgtcaaca ccacgttggg tgacttttac acccgctaaa ggccagcgga
     2401 tttcatcgat gtccagcgaa tcgatgactc ttccctttga acgaagtaac gcgcctggct
     2461 caaaggtcag cgttgggtca gacagacttc cacttagctg cgcaggtaaa cgggcatata
     2521 aaatgaggtc cgcctgtttc gcttcaccgg tcagctgcag aggcagttga ctgttatcca
     2581 tgcttaattt tcctgggcca aaattaagca ccgcgttgcc cttacccgct tgcccctggg
     2641 tcagcacatt cagtcgtccg ctgaccagag cgttctcaag ccctgcctgc cagttgtcga
     2701 ctttgacacc cagtcggcca ctcaaaggaa aaccagcata cagccagctc cagcgaccat
     2761 cgcttacggt caattgttga cgagtaattt gccacggcaa atcgagcaac ggatcgccgt
     2821 tatcccgtgc cagcacgatc aattgcccgc tattttcctg ccagtccagc tcggcatcca
     2881 ccagtgacgg ttcctgcggc aagtttaacg tcgcagtagc atgcccactt acaggaagtc
     2941 catccggcac gagcggcata gtaaattccc ccaccagttt aaccggcggc tgattttcaa
     3001 acgcgacgac atccagttcg ctgactgtaa gttgttgccc tttcagctgg ccttgaaatt
     3061 taactttttc gccctgataa cgcagttgct ggatatcaga ggttaatgcg agagagagtt
     3121 ttccctgcca ttcctgccag ggagaaaaaa tcagtttatc gatattgatc caggtgttag
     3181 gcagcatgga ctgccactgc gcgagggttt ttggagcagc tggcgattgc tccgtctgcg
     3241 gcaatttcgc cagacaagca gaatcaagtt ctaccgtgcc gacgttcaat aaccagcggc
     3301 tgggatgtga aaggctggcg ttggtgatat gcgcaagctg acaatctccc accagataac
     3361 ggagatcggg aatgattaaa cctttacgcg taatgcgtgg gctttcatct aatgcaatac
     3421 gtgtcccgag cggtagccag atgtccgcca gcgtgggaac ccacagcccg agcgtcatca
     3481 gcagcgtcaa cggcacaaga ataatcagta ataacagcgc gagaacggct ttatatttac
     3541 ccagcatggg tagttaatat cctgatttag cgaaaaatta agcattcaat acgggtattg
     3601 tggcatgttt aaccgttcag ttgaaggttg cgcctacact aagcatagtt gttgatgaat
     3661 ttttcaatat cgccatggct ttcaattata tttgaaattt tgtaaaatat ttttagtagc
     3721 ttaaatgtga ttcaacatca ctggagaaag tcttatgaaa ctcgccgttt atagcacaaa
     3781 acagtacgac aagaagtacc tgcaacaggt gaacgagtcc tttggctttg agctggaatt
     3841 ttttgacttt ctgctgacgg aaaaaaccgc taaaactgcc aatggctgcg aagcggtatg
     3901 tattttcgta aacgatgacg gcagccaccc ggtgctggaa gagctgaaaa agcacggcgt
     3961 taaatatatc gccctgcgct gtgccggttt caataacgtc gaccttgatg cggcaaaaga
     4021 actggggctg aaagtagtcc gtgttccagc ctatgatcca gaggccgttg ctgaacacgc
     4081 catcggtatg atgatgacgc tgaaccgccg tattcaccgc gcgtatcagc gtacccgtga
     4141 cgctaacttc tctctggaag gtctgaccgg ctttactatg tatggcaaaa cggcaggcgt
     4201 tatcggtacc ggtaaaatcg gtgtggcgat gctgcgcatt ctgaaaggtt ttggtatgcg
     4261 tctgctggcg ttcgatccgt atccaagtgc agcggcgctg gaactcggtg tggagtatgt
     4321 cgatctgcca accctgttct ctgaatcaga cgttatctct ctgcactgcc cgctgacacc
     4381 ggaaaactac catctgttga acgaagccgc cttcgatcag atgaaaaatg gcgtgatgat
     4441 cgtcaatacc agtcgcggtg cattgattga ttctcaggcg gcaattgaag cgctgaaaaa
     4501 tcagaaaatt ggttcgttgg gtatggacgt ctatgagaac gaacgcgatc tgttctttga
     4561 agataaatcc aacgacgtga tccaggatga cgtattccgt cgcctgtctg cctgccacaa
     4621 cgtgctgttt accgggcacc aggcattcct gacagcagaa gctctgacca gtatttctca
     4681 gactacgctg caaaacttaa gcaatctgga aaaaggtgaa acctgcccga acgaactggt
     4741 ttaatcttgc agctcccctg caatccaggg gagctgattc agataatccc caatgacctt
     4801 tcattctcta ttcttaaaat agtcctgagt cagaaactgt aattgagaac cacaatgaag
     4861 aaagtagccg cgtttgttgc gctaagcctg ctgatggcgg gatgtgtaag taatgacaaa
     4921 attgctgtta cgccagaaca gctacagcat catcgctttg tgctggaaag cgtaaacggt
     4981 aagcccgtga ccagcgataa aaatccgcca gaaatcagct ttggtgaaaa aatgatgatt
     5041 tccggcagca tgtgtaaccg ctttagcggt gaaggcaaac tgtctaatgg tgaactgaca
     5101 gccaaagggc tggcaatgac ccgtatgatg tgcgctaacc cgcagcttaa tgaactcgat
     5161 aac
//
LOCUS       DAZPAB010000238         5150 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZPAB010000238 DAZPAB010000000
VERSION     DAZPAB010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.239"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            189..446
                     /locus_tag="ACHUPG_003722"
                     /pseudo
     CDS             189..446
                     /locus_tag="ACHUPG_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(496..1446)
                     /gene="uspE"
                     /locus_tag="ACHUPG_003723"
     CDS             complement(496..1446)
                     /gene="uspE"
                     /locus_tag="ACHUPG_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR0863138.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1598..2350)
                     /gene="fnr"
                     /locus_tag="ACHUPG_003724"
     CDS             complement(1598..2350)
                     /gene="fnr"
                     /locus_tag="ACHUPG_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR0863139.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2545..3060)
                     /gene="ogt"
                     /locus_tag="ACHUPG_003725"
     CDS             complement(2545..3060)
                     /gene="ogt"
                     /locus_tag="ACHUPG_003725"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR0863140.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTRYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3071..3214)
                     /locus_tag="ACHUPG_003726"
                     /pseudo
     CDS             complement(3071..3214)
                     /locus_tag="ACHUPG_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            3214..3430
                     /locus_tag="ACHUPG_003727"
                     /pseudo
     CDS             3214..3430
                     /locus_tag="ACHUPG_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885449.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(3604..4419)
                     /locus_tag="ACHUPG_003728"
     CDS             complement(3604..4419)
                     /locus_tag="ACHUPG_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0863141.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4516..>5150
                     /locus_tag="ACHUPG_003729"
     CDS             4516..>5150
                     /locus_tag="ACHUPG_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR0863142.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1374 a         1174 c         1225 g         1377 t
ORIGIN      
        1 ctattttacc gcgacaattc attcagaaca tcaatagtca gggaaggaag tagcaacatt
       61 agctaactaa tcaatgcgtt gattgtaaat ccagctaaga ggtgaggttt tcagagcaga
      121 caacggtgaa acgtcatggt attgttacgt ttaggtaaca agaaatttgt ctgcacaagg
      181 attacatcat gattatggcg aaactgaagt cagcgaaagg gaagaaattt ctctttggtt
      241 tgttggcggt tttcattatt gcggcgtcgg ttgtgactcg cgcgaccatc ggcggcgtta
      301 tagaacagta caatattccg ctgtctgagt ggacgacatc aatgtatgtg atttagtcat
      361 cgatgatttt tgtttatagc ctggtcttta ctgtgttgct ggcaatcccg ttgggaattt
      421 atttccttgg cggcgaagag cagtaagtaa aaaataggcc cgataactcg ggccttgtca
      481 gttattgaag agtcgttaat cgtcttcttc gtcatccagt tcaacgggtg tctgatactg
      541 gtcaggttta atgaccagca ggtcgcagcg aagatgatca atcacctgtt ccgccgtgtt
      601 gccgaggaat gctgctgaaa taccggtgcg tccgaccgtg cccagaacca caattcccgc
      661 ctgtaaatgc tccgccaaat caggaatcac ctcttcgggc agaccttttt ctacgtgcgt
      721 catgttttca ttaatgccga atttctgccg cagggctttc attgccagca aatgttgccc
      781 acgaatggca tcgttataaa cgctcgggtc aaattccggc agttcaatcg cgatattaat
      841 tggcgttacc ggataagcgc caaccagatg aacttcggta tggttgactt gttctgccag
      901 ttcgatcgtc tctttgacca gtttttcatt gagcgcatta tgatacggct cttcactggc
      961 gagattcacc gccaccagcg ccttgcctcc ttccggccac ggctggtctt tcaccatcca
     1021 caccgggctt gggcatttgc gtaacagatg ccagtccgtt ggcgtaaaaa tcaccgcttc
     1081 cagacggtca tgttggtgcg ccatttttag caccaaatcg tgtccgccgc tgatcacttc
     1141 ctgaatgatg gcttcgaaag gacggttatg ccagaccact ttaatttcaa tgggaacgcc
     1201 agcattgaga taatattttg cctgctcgtg gatccaggct gtacgctggc tgatgacgcc
     1261 ctgacgcata gcggtacgtt cgtccgggga gagcagggtg gtcatttcgt atgagaagtc
     1321 atagatcggc aaaaaggctt taattttgcc accaatccgt tgatgtaaat aaacagctcg
     1381 ccgcaatgct ggttggtcgt cctggttagg atcgataaca acgagcatgt tctgatacat
     1441 agccatacag ggtctcctta caacaactgt caacgcagtt tgtaattaaa agattaaccc
     1501 atatctggtg aatgaaacag tgatgaacct tctgccagat caataaatca gaaaaattta
     1561 atgatatgac agaaggatag tgagttatgc ggaaaaatca ggcaacgtta cgcgtatggc
     1621 cagcaagctg ggccagcgca tcgttatttt cgatggtgat gtatttacct ttgactgcca
     1681 gcataccgct tttctggaag cgacccagca gacggctgat ggtttctacc gtcaggccca
     1741 gatagttacc aatatcgcca cgagtcatcg tcaggcggaa ttcacgaggg gagaagccgc
     1801 gttgggcaaa acgacgggac aggttgtaga tgaatgcagc cagacgttcc tcggcatttt
     1861 tcttcgacaa cagcaagatc atgtcctgat cgcctttgat ttcaccgctc atcagacgca
     1921 tcatctgctg acgcagattc ggcattttac cggacaaatc gtccagcgtt tcgaacggaa
     1981 tttcacatac catcgaggtt tccagcgcct gagcgaagct cgggtgatgg ccgctgccga
     2041 tggcgtcaaa tcccactagg tcgcctgcta aatggaaacc agtgatttgc tcgtcgcctt
     2101 gctcagtgat tgtataactt ttaatcgtac cggagcggat ggcataaagc gatttaagtt
     2161 catcaccagc cttaaacagc gtctggcctt tctgaatagg cttcttccgc tcaatgatat
     2221 tatcaagctg atcaagctca tgttcgttga gtgtgaacgg gatgcaaagc tggctgatgc
     2281 tgcaatcctg gcaatggata gcacaaccgc cagactgaat gcgccgtata attcgctttt
     2341 ccgggatcat aggtctgctc aagccgtaat tgatatttgt caattttaac atctttttag
     2401 ggagcaagta agtctaagca aaccttaaca gcagagaatt ccgatattag atgtaaatat
     2461 atgtctatct atttgaaact ccttaagttg tcaagggtaa ctttacataa aagtgtgaac
     2521 aagctggcac aaattgttta atgtttacag caaaagataa ccttcgtggc gcaataacca
     2581 ctcttttcgc tgaactccgc ctgcatatct ggtcatggtg ccgtttcggc caataacccg
     2641 atggcaaggt acgacgatgc tgatgggatt cgatccgttt gccgcaccaa cggcacgcgc
     2701 cgcgccaggg cggcccaatt gttcagccag ttggccgtaa tgcattacct gcccgcaggg
     2761 gatagtgcgt agtgttttcc agacttcgcg ctgaaatggc gtcccccccg tggcagtggg
     2821 aagcgtatca ataatgctaa gattaccggc aaaatattca cgaagcttgt cgcttaaacc
     2881 acctgggttg gtggcagaaa tgcgctcata gccttctttg cgataatgga tgtccagcag
     2941 ctgcaccatg cgttcgctgt actcttccca ttcaaccgcc cgcaggcgaa attgctcatc
     3001 gcaaatcacc cacagtggac ccagtggcgt ggcaattttt tcttcaagta atctcagcat
     3061 ccgttctctc ttaagacaaa cgtgggtaaa taccctgacc tatcggcaga ccgacaagat
     3121 accacgccag caacatcagc agccatacca ccaaaaagat aagtggatag ggcaagacta
     3181 acgaatagta agtacccagt ttcgcgtctg gtttgtcgca gtctgatagc ataaagcgat
     3241 tttttaattt ttctgcctga agaaatgagg gcaagccatc acatggatcg agggtgggat
     3301 aattccagtc agtgaaattt taccgtaggc tatctgttat ttgattctgt ggcgtgatag
     3361 ccgccagaca ccactgaact cgtcactcat tccccaatcc cggcgagaat gtggttattt
     3421 acagagctaa aaaatgaata aaaaaataga gcctgtcgac atccgccaga ctctacagta
     3481 cacacagcag tgcatcctcg tcttaaatcc ggcctggggt cctggtgacc ggtgacagtg
     3541 ttataacggt tttacccatc cgttgccaat tttcttcagt gacagatttc acgaaaatat
     3601 aagttaaatc atttgtcttg tgattgcata attatgcatc gttaatcgac gagtaaattt
     3661 accgggcgag tagcccgttc gacgcttaaa catattgccg aacgattcag ctgaggcata
     3721 gcccagacga tgggcaattt tggtcaacga atatccatca tcaagaagcg tcactgcaga
     3781 aataacgtgc atttgctgaa cccagtggcg aaatgttaat cccgtctctt taacaaataa
     3841 gcgcatgagt gtacggctac tgacgttaat tttatccgct agattatcca gtgttggcag
     3901 atacccttgg ttgtttagca aattctcaca aagctgctgt agccgctcat catttggcca
     3961 agggagttca agtgacattt tcacccccat ttttatttcc tgaagtatca gtgtctcgag
     4021 ggctgaaaaa cgtaactgtt gtttatctgt ggtactttgc gacggtggaa gttggttaat
     4081 tgcaattagt aattccctta acaggggtga aatacccgtc atgaagcaaa ctgttcccag
     4141 cgtttcactt tccgcttctg aaaccagtac ggtttttaaa agtacgttgc ctgtcgcaac
     4201 aaacttatgc ggcatttgtg ggggcagcca aagcgcgctg ctggcatgga ctagccagac
     4261 gctttctcca gcccatacac gaacgactcc ttcagccggg taaagcaact gggcatagag
     4321 atgtttatgc cagggaactg tacctccatt cagatgttcc gaatccagtc cgataatgtg
     4381 atgccctggt gatgctttgg gttgcagtgt aattgtcatt tacagtaggt attttgtcag
     4441 aaatcatcaa ataagccatg ttaaatttta aatatgacgt atgcaagttt ccggatatat
     4501 acggtgtaaa gagtgatgat aatttctaaa aaacagctta ttggggtggt agcgatcggt
     4561 attcttttga ctggcgttgt cttttttatc tggagggtaa gcaaaggaag atttatacag
     4621 acgacggatg atgcttacat tggtggtaat atcaccacgg tggcaagtaa agtttctggt
     4681 tatatttctg ctatagaggt gcgcgataat cagagcgtaa aaaaaggcga tattatcctg
     4741 cgtctcgatg atcgggatta tcgtgcaaat gtcgccaggc ttgaggcaaa aataaaaagt
     4801 agcaaagcca atctcgaagg cattcaagcc actattacga tgcagcagtc gataattcaa
     4861 agtgccagtg aaacatggca ggcagtaaaa catgaggagc aaaagcgcct gcgagatact
     4921 gaacgttatg aaaaactcgc acaatcagcg gcaatatcgc aacaaattat tgataacgcc
     4981 aggtttgact accagcaggt cgcagcaaag gagcgcaaag ccgcgaacga tttcctggtg
     5041 gagaagcaac ggctggcagt tttatctgct caggaagaaa acgtacgtgc gagtatcgaa
     5101 gaagttcagg ccgctttgac tcaggcacta ctggatctgg aatataccct
//
LOCUS       DAZPAB010000239         5133 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZPAB010000239 DAZPAB010000000
VERSION     DAZPAB010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.240"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..244
                     /locus_tag="ACHUPG_003730"
     CDS             <1..244
                     /locus_tag="ACHUPG_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863143.1"
                     /translation="EMLSFLTSEEREALTNMLARKGVETVLRLLDKETQELLQLEGRK
                     RLAALMLVDMPDEQVREILEEIEASILSESVNAKAG"
     gene            493..1437
                     /gene="tauA"
                     /locus_tag="ACHUPG_003731"
                     /pseudo
     CDS             493..1437
                     /gene="tauA"
                     /locus_tag="ACHUPG_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
     gene            1450..2217
                     /gene="tauB"
                     /locus_tag="ACHUPG_003732"
     CDS             1450..2217
                     /gene="tauB"
                     /locus_tag="ACHUPG_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR0863144.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            2214..3041
                     /gene="tauC"
                     /locus_tag="ACHUPG_003733"
     CDS             2214..3041
                     /gene="tauC"
                     /locus_tag="ACHUPG_003733"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFR0863145.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            3038..3889
                     /gene="tauD"
                     /locus_tag="ACHUPG_003734"
     CDS             3038..3889
                     /gene="tauD"
                     /locus_tag="ACHUPG_003734"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR0863146.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACHUPG_003735"
     CDS             complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="ACHUPG_003735"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR0863147.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1174 a         1301 c         1466 g         1192 t
ORIGIN      
        1 agaaatgttg tcgtttctta ccagtgagga acgagaagcc cttacaaaca tgctcgcaag
       61 gaaaggtgtt gagactgtgc tacgcttgtt agataaggaa actcaagagc tactgcaact
      121 agagggccgt aaacgcctgg cagcgttgat gctcgttgat atgcccgacg agcaagtcag
      181 agagattttg gaagagattg aagctagtat tctctcagag agtgtaaacg cgaaggcggg
      241 ttaacttcat tgacctgcct ttagggtgcc tttaaaattc gtttttaaag gctacgatga
      301 catttttgaa gagtcttaaa agaattaaaa tacacggcgc gcctatcttg aatgttttat
      361 cgatatcgaa gcattgagca tttttaactc gttttatctt gaatgcaatc ggcgcataac
      421 ttattgaatc ccatcaaatc ccgtcttttt ttacccattc ccggttatct tgaataaatt
      481 acctagtcat ataacacact tcttgccgca ctggcattca tcgcttttca ggcacaggcg
      541 gtgaacgtca ccgtggcgta tcaaacctca gccgaaccgg cgaaagtggc tcaggccgac
      601 aacacctttg ctaaagagag cggggcgagc gtggactggc gtaagtttga cagcggagcc
      661 agcatcgtgc gggcgctggc ttctggcgac gtgcaaatcg gcaacctcgg ttccagccca
      721 ttagcggttg cagccagcca acaggtgccg attgaagtct tcttgctggc gtcaaaactg
      781 ggtaactccg aagcgctggt ggtaaagaaa actatcagca aacccgaaga tttgattggt
      841 aaacgcattg ccgtaccgtt tatctccacc acccactaca gcctgctggc ggcgctgaaa
      901 cactggggga ttaaaccagg gctagtggag attgtgaacc tgcagccgcc cgcgattatc
      961 gccgcatggc agcggggtga tattgatggt gcttatgtct gggcaccggc ggttaacgcc
     1021 ctggaaaaag acggcaaggt gctgaccgat tctgaacagg tcgggcagtg gggtgcgcca
     1081 acgctggacg tctgggtggt gcgcaaagat tttgccgaga aacatcctga ggtcgtgaaa
     1141 gcgttcgcca aaagcgccat cgatgcacag caaccgtaca ttgctaaccc tgacgcgtgg
     1201 ctgaaacagc cggaaaacat cagcaaactg gcgcgtttaa gcggcgtgcc tgaaggtgac
     1261 gttccggggc tggtgaaggg aaatacctat ctgacgccgc agcaacaaac ggcagaactg
     1321 accggaccgg tgaacaaagc aatcatcgac accgcgcagt ttttgaaaga gcagggcaag
     1381 gtcccggctg tagcgaatga ttacagccag tacgttacct cgcgcttcgt gcaataaaag
     1441 gaggcgcaga tgctgcaaat ctctcatctc tatgccgatt atggcggcaa accggcactg
     1501 gaagatatca acctgacgct ggaaagcggc gagctactgg tggtgctggg gccgtccggc
     1561 tgcggtaaaa ccaccctgct gaatctgatt gccggttttg tgccttatca gcatggcagc
     1621 attcaactgg cgggtaagcg tattgaggga ccgggagcag agcgtggcgt agtttttcag
     1681 aatgaagggc tgctaccgtg gcgcaatgta caggacaacg tggcgttcgg cctgcaattg
     1741 gcaggtatag agaaaatgca gcgactggaa atcgcgcacc agatgctgaa aaaagtgggg
     1801 ctggaaggcg cagaaaaacg ctatatctgg cagctttccg atggtcaacg tcagcgggtg
     1861 gggattgccc gcgcgctggc ggcgaatccc cagctgttat tactcgacga accgtttggt
     1921 gcgctggacg ccttcacccg cgaccagatg caaactctgc tgctgaaact ctggcaggag
     1981 acgggcaagc aggtgctgtt gattacccac gatatagaag aagcggtgtt tatggcgact
     2041 gaactggttc tgctttcatc cggccctggc cgtgtgctgg agcggctgcg gctcaacttt
     2101 gctcgccgct ttgttgcggg agagtcgagc cgcagcatca agtccgatcc acaattcatc
     2161 gccatgcgcg aatatgtttt aagccgcgta tttgagcaac gggaggcgtt ctcatgagtg
     2221 tgctcattaa tgaaaaactg cattcgcggc ggctgaaatg gcgctggccg ctctcgcgtc
     2281 aggtgacctt aagcattggc acgttagcgg ttttactcac tgtatggtgg acggtggcgg
     2341 cgctgcaact gattagcccg ctgtttttgc cgccgccgca acaagtactg gcaaaactgc
     2401 tcaccattgc cggaccgcag ggatttatgg acgccacgct gtggcagcat ctggcagcca
     2461 gcctgacgcg cattgtgctg gcgctactcg cggcggtggt gatcggtatt ccggtcggga
     2521 tcgccatggg gctaagcccg acggtgcgcg gcattctgga tccggtaatc gaactttatc
     2581 gtccggtgcc accgctggct tatttgccgc tgatggtgat ctggtttggt attggtgaaa
     2641 cctcgaagat cttactgatc tatttagcga tttttgcgcc ggtggcgatg tcggcgctgg
     2701 caggggtgaa aagcgcgcag caggttcgca ttcgtgccgc ccagtcgctg ggtgccagcc
     2761 gtgcgcaggt gctgtggttt gtcattttgc ccggcgcgct gccggaaatc ctcaccggat
     2821 tacgcattgg tctgggtgtg ggctggtcta cgctggtagc ggcggagctg attgccgcga
     2881 cacgcggttt aggatttatg gttcagtcag cgggtgaatt tctcgcgact gacgtggtac
     2941 tggcggggat cgcggtgatt gcgattatcg cctttctttt agaactgggt ctgcgcgcgt
     3001 tacagcgccg tctgacgccc tggcatggag aagtacaatg agtgaacgtc tgagcattac
     3061 cccgctgggg ccgtatatcg gcgcacaaat ttcgggtgcc gacctgacgc gcccgttaag
     3121 cgataatcag tttgaacagc tttaccatgc ggtgctgcgc catcaggtgg tatttctgcg
     3181 cgatcaagct attacgccgc agcagcaacg cgcgctggcc cagcgttttg gcgaactgca
     3241 tattcaccct gtttaccctc atgccgaagg ggttgacgag atcatcgtgc tggataccca
     3301 taacgataat ccgccggata acgacaactg gcataccgat gtgacattta ttgaaacgcc
     3361 acccgcaggg gcgattctgg cagctaaaga gttaccttcg accggcggtg atacgctctg
     3421 gaccagcggt attgcggcct atgaggcgct ctctgttccc ttccgccagc tgctgagctg
     3481 gctgcgtgcg gagcatgatt tccgtaaatc attcccggaa tacaaatacc gcaaaaccga
     3541 ggaggaacat caacgctggc gcgaggcggt cgcgaaaaat cctccgttgc tacatccggt
     3601 ggtgcgaacg catccggtga gcggtaaaca ggcgctgttt gtgaatgaag gctttactac
     3661 gcgaattgtc gatgtcagcg agaaagagag cgaagccttg ttaggctttt tattcgccca
     3721 tatcactaaa ccggagtttc aggtgcgttg gcgctggcaa ccgaatgata ttgcgatttg
     3781 ggataaccgc gtgactcagc actatgccaa tgccgattac ctgccacagc ggcggattat
     3841 gcatcgggcg acgatccttg gggataaacc gttttatcgg gcggggtgat acgagtgcgg
     3901 acggttccct cgcccccttg gggagagggt tagggtgagg gggcgttcac tgtactttca
     3961 acaggttaac tccccctttc tgagaggaaa aaaattaacg cagaatcttc ttctcagcca
     4021 aatccagcgc aaagtagctg aaaatcagat ccgcacccgc acgcttaatt gaacctaagc
     4081 tttcgagcac gactttctct tcatctatag cacccgccag cgcggcgaac ttaatcatcg
     4141 cgtactcacc gctcacctga tacgcgccaa tcggcaattc agtacgttca cgcagctcac
     4201 gcacgatgtc gaggtacgct ccagcaggtt taaccatcag gcagtctgcg ccctgggctt
     4261 catccagcag tgactcacga atcgcctcgc gacggttcat tgggttcatc tgatagcttt
     4321 tgcggtcgcc ttttaatgcg cttcctgcag cttcacggaa cgggccataa aaggaagagg
     4381 cgaacttggt cgaatacgac ataatcgccg tatctttaaa gcctgcagcg tccagcgcct
     4441 gacgaatcgc ctgtacctgg ccgtccatcg cggcggaagg ggcgatgaag tctgcgcctg
     4501 cagcagctgc aaccacggct tgcttgccta aattttccag agtcgcgtcg ttgtcgacgc
     4561 catgctcgca cagcacaccg cagtgaccgt gagaagtgta ttcacagaaa caagtgtctg
     4621 acatgacgat catttctggc acagtctgct tgcagatgcg cgacatacgc gccaccagtc
     4681 catcttcccg ccaggcgtcg ctgccggttt catcggtatg gtgagagatg ccaaaagtca
     4741 ttacggaacg gataccggcg ttggcgatgc gttcaatttc gcgtgccaga tgtttctctg
     4801 gaatgcgcat cacgcctggc atggcttcaa cggctttgta gtcgtcaatt tcttcttcaa
     4861 caaagatcgg caacaccagg tcgttaaggc taagtgttgt ctcttcaaac atagcgcgca
     4921 gcgcaggaga tttgcgcagg cgacgagggc gttggattaa gtctgtcatg gtctgcctga
     4981 tgtttgtgga attaaggtgc acagtatacc tgaagcgggg caggagtgtt ttacgaaagt
     5041 tgtcgcgatg ttgacaagaa gagaatgaaa gagagaccag gacatctttg taatagaaat
     5101 agcaataatt tcatattcac tgaaaatatt ttt
//
LOCUS       DAZPAB010000240         5094 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZPAB010000240 DAZPAB010000000
VERSION     DAZPAB010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.241"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(11..322)
                     /locus_tag="ACHUPG_003736"
     CDS             complement(11..322)
                     /locus_tag="ACHUPG_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR0863148.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="ACHUPG_003737"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHUPG_003737"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="ACHUPG_003738"
     CDS             701..1054
                     /locus_tag="ACHUPG_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFR0863149.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACHUPG_003739"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACHUPG_003739"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFR0863150.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="ACHUPG_003740"
     CDS             complement(2810..3280)
                     /locus_tag="ACHUPG_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR0863151.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACHUPG_003741"
                     /pseudo
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACHUPG_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4119..4337)
                     /locus_tag="ACHUPG_003742"
     CDS             complement(4119..4337)
                     /locus_tag="ACHUPG_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR0863152.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACHUPG_003743"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACHUPG_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR0863153.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         1376 a         1176 c         1219 g         1323 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtgcc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gaactatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttactgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg atta
//
LOCUS       DAZPAB010000241         4978 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZPAB010000241 DAZPAB010000000
VERSION     DAZPAB010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.242"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            250..1194
                     /gene="leuO"
                     /locus_tag="ACHUPG_003744"
     CDS             250..1194
                     /gene="leuO"
                     /locus_tag="ACHUPG_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR0863154.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            complement(1349..1393)
                     /locus_tag="ACHUPG_003745"
     CDS             complement(1349..1393)
                     /locus_tag="ACHUPG_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863155.1"
                     /translation="MKRNDKILHYEGLN"
     gene            1512..3236
                     /gene="ilvI"
                     /locus_tag="ACHUPG_003746"
     CDS             1512..3236
                     /gene="ilvI"
                     /locus_tag="ACHUPG_003746"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR0863156.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3239..3730
                     /gene="ilvN"
                     /locus_tag="ACHUPG_003747"
     CDS             3239..3730
                     /gene="ilvN"
                     /locus_tag="ACHUPG_003747"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR0863157.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            3742..3828
                     /locus_tag="ACHUPG_003748"
     CDS             3742..3828
                     /locus_tag="ACHUPG_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR0863158.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            3910..4914
                     /gene="cra"
                     /locus_tag="ACHUPG_003749"
     CDS             3910..4914
                     /gene="cra"
                     /locus_tag="ACHUPG_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR0863159.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT         1210 a         1134 c         1311 g         1323 t
ORIGIN      
        1 attgagtatt cgcggtagtt atgattagat tgttttcgca acaaaacatt atggattatt
       61 atgctgtggt aaatgactca ttccacggca atggattctg tttttatcag aacccgtatc
      121 tttatgtttt ccgaatttta ctcattttgc tttttcttat tttatatgca tgataaatca
      181 tattcttcag gattatttct ctgcattcca ataagggaaa gggagttaag tgtgacagtg
      241 gagttaagta tgccagaggt acaaacagat cattcagaga cggcggagtt aagcaagcca
      301 cagctacgca tggtcgatct caacttatta accgttttcg atgccgtgat gcaggagcaa
      361 aacattaccc gtgccgctca tgttctgggt atgtcgcaac ctgcggtcag taacgctgtt
      421 gcacgcctga aggtgatgtt taatgacgag ctttttgttc gttatggccg tggtattcaa
      481 ccgactgctc gcgcatttca actttttggt tcagttcgcc aggcattgca actagtacaa
      541 aatgaattgc ctggttcagg ttttgaaccc gcgagcagtg aacgtgtatt tcatctttgt
      601 gtttgcagcc cgttagacag cattctgacc tcgcagattt ataatcacat tgagcagatt
      661 gcgccaaata tacatgttat gttcaagtct tcattaaatc agaacactga acatcagctg
      721 cgttatcagg aaacggagtt tgtgattagt tatgaagact tccatcgtcc tgaatttacc
      781 agcgtgccat tatttaaaga tgaaatggtg ctggtagcca gcaaaaatca tccaacaatt
      841 aaaggcccgt tactgaaaca tgatgtttat aacgaacaac atgcggcggt ttcgctcgat
      901 cgtttcgcgt catttagtca accttggtat gacacggtag ataagcaagc cagtatcgcg
      961 tatcagggca tggcaatgat gagcgtactt agcgtggtgt cgcaaacgca tttggtcgct
     1021 attgcgccgc gttggctggc tgaagagttc gctgaatcct tagaattaca ggtattaccg
     1081 ctgccgttaa aacaaaacag cagaacctgt tatctctctt ggcatgaagc tgccgggcgc
     1141 gataaaggcc atcagtggat ggaagaacaa ttagtctcaa tttgcaaacg ctaactgaat
     1201 gcagaatagg ccagacatga atgtctggtt tattctgcat tttttattga atgtagaatt
     1261 ttattctgaa tgtgtggact ctctatttta ggattaatta aaaaaataga gaaattgctg
     1321 taagttgtgg gattcagccg atttattatc aatttaatcc ttcgtaatgg aggattttat
     1381 cgtttctttt cacctttcct cctgtttatt cttattaccc tgcgtttatg tctctggctg
     1441 ccaattgctt aagcaagatc ggacggttaa tgtgttttac acattttttc cgtcaaacag
     1501 tgaggcaggc catggagatg ttgtctggag ccgagatggt cgtccgatcg cttatcgatc
     1561 agggcgttaa acaagtattc ggttatcccg gaggcgcagt ccttgatatt tacgatgcat
     1621 tgcataccgt gggtggtatt gatcatgtat tagttcgtca tgagcaggcg gcggtgcata
     1681 tggccgatgg cctggcgcgt gcgaccgggg aagtcggcgt cgtgctggta acgtcgggtc
     1741 caagggcgac caatgcgatt accggcatcg ccaccgctta tatggattcc attccattag
     1801 ttgtcctttc cgggcaggta gcgacctcgt tgataggtta cgatgccttt caggagtgcg
     1861 acatggtggg gatttcgcga ccggtggtta aacacagttt tctggttaag caaacggaag
     1921 acattccgca ggtgctgaaa aaggctttct ggctggcggc aagtggtcgc ccaggaccag
     1981 tggtcgttga tttaccgaaa gatattctta atccggcgaa caaattaccc tatgtctgcc
     2041 ctgagtcggt cagtatgcgt tcttacaatc ccactactac cggacataaa gggcaaatta
     2101 agcgtgctct gcaaacgctg gtagcggcaa aaaaaccggt tgtctacgta ggcggtgggg
     2161 caatcatggc gggctgccat cagcagttga aagaaacggt ggaggcgttg aatctgcccg
     2221 ttgtttcctc attgatggga atgggggcgt ttccggcaac gcatcgtcag gcactgggca
     2281 tgctgggaat gcacggtacc tacgaagcca atatgacgat gcataacgcg gatgtgattt
     2341 tcgccgtcgg ggtacgattt gatgaccgaa cgacgaacaa tctggcaaag tactgcccaa
     2401 atgccactgt tctgcatatc gatattgatc ctacttccat ttctaaaacc gtgactgcgg
     2461 atatcccgat tgtgggggat gctcgccagg tcctcgaaca aatgcttgaa ctcctgtcgc
     2521 aagaatccgc ccatcaaccg ctggatgaga tccgcgactg gtggcagcag attgaacagt
     2581 ggcgcgctct tcagtgcctg aaatatgaca ctcacagtga aaagattaaa ccgcaggcgg
     2641 tgatcgagac tctttggcgg ttgactaatg gggacgctta cgtgacgtcc gatgtcgggc
     2701 agcaccagat gtttgctgcg ctttattatc cattcgacaa accgcgtcgc tggatcaatt
     2761 ccggcggtct cggcacgatg ggttttggtt tacctgcggc actgggcgtc aaaatggcgt
     2821 tgccagaaga aaccgtggtt tgcgtcactg gcgacggcag tattcagatg aacatccagg
     2881 aactgtctac cgcgttgcaa tacgagttgc ccgtactggt ggtgaatctc aataaccgct
     2941 atctggggat ggtgaaacag tggcaggaca tgatctattc cggccgtcat tcacaatctt
     3001 atatgcaatc actacccgat ttcgtccgtc tggcggaagc ctatggtcac gtcgggatcc
     3061 agatctctca tccgcatgag ctggaaagca aacttagcga ggcgctggaa caggtgcgca
     3121 ataatcgcct ggtgtttgtt gatgttaccg tcgatggcag tgagcacgtc tacccgatgc
     3181 agattcgcgg gggcggaatg gatgaaatgt ggttaagcaa aacggagaga acctgattat
     3241 gcgccggata ttatcagtct tactcgaaaa tgaatcaggc gcgttatccc gcgtgattgg
     3301 ccttttttcc cagcgaggct acaacattga aagcctgacc gttgcgccaa ccgacgatcc
     3361 gacattatcg cgtatgacca tccagaccgt gggcgatgaa aaagtacttg agcagatcga
     3421 aaagcaatta cacaagctgg tcgatgtctt gcgcgtgagt gagttggggc agggcgcgca
     3481 tgttgagcgg gaaatcatgc tggtgaaaat tcaggccagc ggttacgggc gtgacgaagt
     3541 gaaacgtaat acggaaatat tccgtgggca aattatcgat gtcaccccct cgctttatac
     3601 cgttcaatta gcaggcacca gcgataagct tgatgcattt ttagcatcga ttcgcgatgt
     3661 ggcgaaaatt gtggaagttg ctcgctctgg tgtggtcgga ctttcgcgcg gcgataaaat
     3721 aatgcgttga gaatgatctc agtgcgcaat ttgcagccca acatgtcacg ttgggctttt
     3781 tttgcgaaat cagtgggaac ctggaataaa agcagttgcc gcagttaatt ttctgcgctt
     3841 agatgttaat gaatttaacc cataccagta caatggctat ggtttttaca ttttacgcaa
     3901 ggggcaattg tgaaactgga tgaaatcgct cggctggcgg gagtgtcgcg gaccactgca
     3961 agctatgtta ttaacggcaa agcgaagcaa taccgtgtga gcgacaaaac cgttgaaaaa
     4021 gtcatggctg tggtgcgtga gcacaattac cacccgaacg ccgtggcagc tgggcttcgt
     4081 gctggacgca cacgttctat tggtcttgtg atccccgatc tggagaacac cagctatacc
     4141 cgcatcgcta actatcttga acgccaggcg cggcaacggg gttatcaact gctgattgcc
     4201 tgctcagaag atcagccaga caacgaaatg cgctgcattg agcacctttt acagcgtcag
     4261 gttgatgcca ttattgtttc gacgtcgttg ccacctgagc atccttttta tcaacgctgg
     4321 gctaacgacc cgttcccgat tgtcgcgctg gaccgcgccc tcgatcgtga acatttcacc
     4381 agcgtggttg gtgccgatca ggatgatgcc gaaatgctgg cggaagagtt acgtaagttt
     4441 cccgccgaga cggtgcttta tcttggcgcg ctgccggagc tttctgtcag cttcctgcgt
     4501 gaacaaggtt tccgtactgc ttggaaagat gatccgcgcg aagtgcattt cctgtatgcc
     4561 aacagctatg agcgggaggc ggctgcccag ttattcgaaa aatggctgga aacgcatccg
     4621 atgccgcagg cgctgttcac aacgtcgttt gcgttgttgc aaggagtgat ggatgtcacg
     4681 ctgcgtcgcg acggcaaact gccttctgac ctggcaattg ccacctttgg cgataacgaa
     4741 ctgctcgact tcttacagtg tccggtgctg gcagtggctc aacgtcaccg cgatgtcgca
     4801 gagcgtgtgc tggagattgt cctggcaagc ctggacgaac cgcgtaagcc aaaacctggt
     4861 ttaacgcgca ttaaacgtaa tctctatcgc cgcggcgtgc tcagccgtag ctaatccgcg
     4921 aacaaaaata cgcgccaggt gaatttccct ctggcgcgta gagtacggga ctggacat
//
LOCUS       DAZPAB010000242         4973 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZPAB010000242 DAZPAB010000000
VERSION     DAZPAB010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.243"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACHUPG_003750"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACHUPG_003750"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFR0863160.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="ACHUPG_003751"
     CDS             1020..1703
                     /locus_tag="ACHUPG_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFR0863161.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="ACHUPG_003752"
     CDS             1700..2161
                     /locus_tag="ACHUPG_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFR0863162.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="ACHUPG_003753"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="ACHUPG_003753"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR0863163.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="ACHUPG_003754"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="ACHUPG_003754"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFR0863164.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACHUPG_003755"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACHUPG_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFR0863165.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACHUPG_003756"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACHUPG_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFR0863166.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(4903..>4973)
                     /locus_tag="ACHUPG_003757"
     CDS             complement(4903..>4973)
                     /locus_tag="ACHUPG_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863167.1"
                     /translation="NYSEYSEYTSVFFLSLMIFIKS"
BASE COUNT         1177 a         1305 c         1238 g         1253 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctgact caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct tttttcccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg cggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggt gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtgag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaaa cgctagtata ttctgaatat tccgaatagt tac
//
LOCUS       DAZPAB010000243         4860 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZPAB010000243 DAZPAB010000000
VERSION     DAZPAB010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.244"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..527
                     /gene="ydcF"
                     /locus_tag="ACHUPG_003758"
     CDS             <1..527
                     /gene="ydcF"
                     /locus_tag="ACHUPG_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR0863168.1"
                     /translation="TTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
     gene            724..2163
                     /gene="aldA"
                     /locus_tag="ACHUPG_003759"
     CDS             724..2163
                     /gene="aldA"
                     /locus_tag="ACHUPG_003759"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFR0863169.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2205..3205)
                     /gene="gap"
                     /locus_tag="ACHUPG_003760"
                     /pseudo
     CDS             complement(2205..3205)
                     /gene="gap"
                     /locus_tag="ACHUPG_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3394..3924
                     /gene="cybB"
                     /locus_tag="ACHUPG_003761"
     CDS             3394..3924
                     /gene="cybB"
                     /locus_tag="ACHUPG_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR0863170.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4169..4342
                     /locus_tag="ACHUPG_003762"
     CDS             4169..4342
                     /locus_tag="ACHUPG_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863171.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            4368..4436
                     /gene="yncP"
                     /locus_tag="ACHUPG_003763"
     CDS             4368..4436
                     /gene="yncP"
                     /locus_tag="ACHUPG_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR0863172.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="ACHUPG_003764"
     CDS             complement(4414..4563)
                     /gene="hokB"
                     /locus_tag="ACHUPG_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR0863173.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4664..4804)
                     /locus_tag="ACHUPG_003765"
                     /pseudo
     CDS             complement(4664..4804)
                     /locus_tag="ACHUPG_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1243 a         1119 c         1207 g         1291 t
ORIGIN      
        1 gcaccactgg cagagcagaa gcgaccatcc tggtggatat cgctcatcag ttctggcata
       61 ttccgcatga aaaaatctgg attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct
      121 ttagcatcac gctattgaat caggccgtag aacgagttca tacggctatc gttgtacagg
      181 accccaccat gcagcggcgc acgatggcaa cgttccgccg tatgaccggg gacaatcccg
      241 atgcaccacg ctggttaagt tatcccggat tcgttcctca gttaggaaat aacgcagaca
      301 gtgtaatctt tattaatcag ttacaaggat tatggccggt tgagcgttat ctctcactac
      361 tcactggcga gctgccgcgt ttacacgatg atagcgatgg ctacggtccc cgcgggcgag
      421 attttatcgt tcacgttgat tttccggcag aagtcatcca tgcatggcaa acgctgaaac
      481 atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt
      541 gtgaaccact tgtttgcaaa cgggcatgac tcctgacttt tatttctgcc ttttattcct
      601 tttacacttg tttttatgaa gccctccaca gacttgtcct ttcccgatgc cgtctctctg
      661 atgattgatg ttaattaaca atatattcac cgaaaacaaa catataaatc acaagagtcg
      721 cccatgtcag tacccgttca acatcctatg tatatcgatg gacagtttgt tacctggcgt
      781 ggagacgcat ggattgatgt ggtaaaccct gctacagagg ctgtcatttc ccgcataccc
      841 gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa
      901 tgggaagcgt tgcctgctat tgaacgcgcc agttggttgt gcaaaatctc cgccgggatc
      961 cgcgaacgcg ccagtgaaat cagtgcgctg attgttgaag aagggggcaa gatccagcag
     1021 ctagctgaag tcgaagtggc ttttactgcc gactatatcg attacatggc ggagtgggca
     1081 cggcgttacg agggcgagat tattcaaagc gatcgtccgg gagaaaatat tcttctgttt
     1141 aaacgtgcgc ttggtgtgac taccggcatt ctgccgtgga actttccgtt cttcctcatt
     1201 gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca tcgtcattaa acccagtgaa
     1261 tttacgccaa acaatgcgat tgcattcgcc aaaatcgtcg atgaaatagg ccttccgcgc
     1321 ggcgtgttta accttgtact ggggcgtggt gaaaccgttg ggcaagaact ggcgggtaac
     1381 ccaaaggtcg caatggtcag tatgacaggc agcgtctctg caggtgagaa gatcatggcg
     1441 actgcggcga aaaacatcac caaagtgtgt ctggaattgg gtggtaaagc accggctatc
     1501 gtaatggacg atgccgatct tgaactggca gtcaaagcca tcgttgattc acgcgtcatt
     1561 aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg tacagaaagg catttatgat
     1621 cagttcgtca atctgctggg tgaagcgttg caggcggttc aatttggtaa ccccgctgaa
     1681 cgcaacgaca ttgcgatggg gccgttgatt aacgccgcgg cgctggaaag ggttgagcaa
     1741 aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt tgggtggcaa agcggtagag
     1801 gggaaaggat attattatcc gccgacattg ctgctggatg ttcgccagga aatgtcgatt
     1861 atgcatgagg aaacctttgg cccggtgctg ccagttgtcg catttgacac gctggaagat
     1921 gctatctcaa tggctaatga cagtgattac ggcctgacct catcaatcta tacccaaaat
     1981 ctgaacgtcg cgatgaaagc cattaaaggg ctgaagtttg gtgaaactta catcaaccgt
     2041 gaaaacttcg aagctatgca aggtttccac gccggatggc gtaaatccgg tattggcggc
     2101 gcagatggta aacatggcct gcatgaatat ctgcagaccc aggtggttta tttacagtct
     2161 taatgagtga aagaggcgga ggttttttcc tccgcctgtg cgcgtcagag tttagcgaat
     2221 ttttcgaggg tgcgaataag ctgcgtgacg aagccatatt cgttatcgta ccaggcgacc
     2281 gttttcacca gttgtaaatc gcccacggcg gtaatttccg tttgcgtggc atcaaacact
     2341 gaaccgaaat ggctgccaat gatatcggaa gagactattt cttcatcggt ataaccaaat
     2401 gactcgttat tggtggttgc ttgtttaagt gcgttattca cctcttcggc agtcactttt
     2461 ttccgagaat ggacaccagc tcagtgaccg aacctgtttt caccggcacg cgttgcgcat
     2521 gacctttcag tttgccgctc agttccggga tcaccagacc aatggctttt gccgcccccg
     2581 tagtgtgggg aatgatattt tctgccgctg cgcgtgaagc acgtaaatct ttaccacgcg
     2641 ggccatccac cagtgactgg gtgccagtat aggcatgaat ggtcgtcatc gtgccgactt
     2701 ctatcccgaa actgtcatgc aaggctttgg ccatcggcgc aaggcagtta gtggtgcatg
     2761 acgccacgga aacaatggtg tcgttgccat ccagagtgtc gtcattaacg ttataaacga
     2821 tggttttcat ttcaccggca ggagcggaaa tcaacacctt cttcgcacca gcatcaagat
     2881 gcgcctgcga tttatcggcg gaggtataaa agccagtaca ttcgacaatg atttctgccc
     2941 ctttcgcttt ccacggaata tttttagcct ctttttcggc gtaaaccgcg atacttttcc
     3001 catcaacgat aagtgaatct tccgtaaaat caacgctcca ggggaacgga ccatagtttg
     3061 aatcatgttt cagcagatag gcgagaattt ttggggaagt aagatcatta atagcgacaa
     3121 cgtctatgtt gcttttgact tcaagtaaac gacgcaacac cagtcgaccg atacgaccaa
     3181 aaccgttaat accaacttta ctcatggttt tctcctgtca ggaacgttcg gatgaaaatt
     3241 gatcctttcc aggcttagac caggattgtg ggatgggcaa tctccattct tacagtgaaa
     3301 cgtaacgtac tgaaaacggg tgaacaatat ttaatgaaat tttgagaaaa gcccgttatg
     3361 ttaacggaaa attatgttaa agcaggaaat gttatggaaa ataaatactc aaggttacaa
     3421 atcagcattc actggctggt ctttttgctg gttatcgcag cgtattgcgc aatggagttt
     3481 cgtggtttct tcccacgtag cgatcggccg ctcatcaaca tgattcatgt ttcctgtggc
     3541 atctcaattc tcgtgctgat ggtggttcgt cttttgttaa gactgaaata cccaaccccg
     3601 ccgattatac ctaagccgaa accgatgatg acgggactgg cgcatttggg acatttggtg
     3661 atttatctcc tgtttattgc gctgccagtg attggtttgg tgatgatgta taaccggggc
     3721 aacccgtggt ttgcgtttgg tttgacgatg ccttacgctt cagaggccaa tttcgaacgg
     3781 gttgatagct taaagtcgtg gcatgagacg ctggcgaatc tgggatattt tgtcatcggg
     3841 ttgcacgctg cggctgcact ggcacaccac tatttctgga aggacaacac acttctacgc
     3901 atgatgccgc gtaaacgttc ctgaaggata tttaaagaaa acgcctgtac taaaaccgac
     3961 ccgtggtaca ggcgaagaat acgggtctac atcggaagcg cctattatat ttatttgtat
     4021 gacaaataaa accataatcc ttgcccatac gtccatctgg cttattttta atcaattcac
     4081 ccgatctttg atctcatcaa cggtatcaaa ataaaccagc gtataacgtt caaatcgcac
     4141 ataagattcg ataaaccatg gaggttatat gaaaaaactg gcacttattt tgtttatggg
     4201 aacgcttgtt tccttttatg ccgatgccgg gcgcaaaccc tgttctggtt cgaaaggggg
     4261 gatctcacac tgtacggcag gcggcaaatt tgtctgtaat gatggttcta ttagtgcatc
     4321 gaaaaaaaca tgcactaact gaagtgtaaa aggggtgcca tgagaaaatg aatctgctag
     4381 tcagatgcgc ggggaaaatc cccgcgcttg cccttacctg gacgtgcagg ccatgagcgc
     4441 agcaacctcc ttatcaccgt cccggaaccg cagttcgtag agcgtttgtc gggtcaggag
     4501 tgtgaatgcc agaatcgtaa tgcagataat gagcagacac accaccagag ggttgcgctt
     4561 catagccttt ctccttgccg gatggcgggt aagaggctaa caatctgaat tgcttggttc
     4621 attcgttggc ctcggtttgt actgacccca aaaagttgga cagttaaaca cgaggcatat
     4681 aggtctgatt ccgatattca tttggagtca gaccttttaa tttcaggcta attcttctgc
     4741 tgttgtagta ttcaatatat tccgtaacag catccttcag ttcgcttata ttactgaact
     4801 cattagactg gccccctgaa tctccagaca accagtatca cttatttaag tgatagtctt
//
LOCUS       DAZPAB010000244         4828 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZPAB010000244 DAZPAB010000000
VERSION     DAZPAB010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.245"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="ACHUPG_003766"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="ACHUPG_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR0863174.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="ACHUPG_003767"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="ACHUPG_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HFR0863175.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="ACHUPG_003768"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="ACHUPG_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR0863176.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="ACHUPG_003769"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="ACHUPG_003769"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR0863177.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="ACHUPG_003770"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="ACHUPG_003770"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR0863178.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="ACHUPG_003771"
     CDS             4449..4814
                     /locus_tag="ACHUPG_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR0863179.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
BASE COUNT         1322 a         1238 c         1094 g         1174 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggctaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt caccttgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 accatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatttgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgctcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acatcccctt taaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaagggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaa
//
LOCUS       DAZPAB010000245         4809 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZPAB010000245 DAZPAB010000000
VERSION     DAZPAB010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.246"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(12..388)
                     /gene="rnpB"
                     /locus_tag="ACHUPG_003772"
     ncRNA           complement(12..388)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHUPG_003772"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(421..1566)
                     /gene="garK"
                     /locus_tag="ACHUPG_003773"
     CDS             complement(421..1566)
                     /gene="garK"
                     /locus_tag="ACHUPG_003773"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR0863180.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1663..2553)
                     /gene="garR"
                     /locus_tag="ACHUPG_003774"
     CDS             complement(1663..2553)
                     /gene="garR"
                     /locus_tag="ACHUPG_003774"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR0863181.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(2583..3353)
                     /gene="garL"
                     /locus_tag="ACHUPG_003775"
                     /pseudo
     CDS             complement(2583..3353)
                     /gene="garL"
                     /locus_tag="ACHUPG_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3369..4703)
                     /gene="garP"
                     /locus_tag="ACHUPG_003776"
     CDS             complement(3369..4703)
                     /gene="garP"
                     /locus_tag="ACHUPG_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR0863182.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1152 a         1376 c         1156 g         1125 t
ORIGIN      
        1 ttacgtaaaa caggtgaaac tgaccgataa gccgggttct gtcgtggaca gtcattcatc
       61 taggccagca atcgctcact ggctcaagca gcctacccgg gttcagtacg ggccgtacct
      121 tatgaacccc tatttggcct tgctccgggt ggagtttacc gtgccacgga ctgttaccag
      181 ccgcgcggtg cgctcttacc gcaccctttc acccttacct gatcccgctt gcacgggcca
      241 tcggcggttt gctctctgtt gcactggtcg tgggtttccc ccccaggcgt tacctggcac
      301 cctgccctat ggagcccgga ctttcctccc ctccacccgt ctcccccgaa gaggacgacg
      361 acgaagcggc gactgtctgg tcagcttcgg cgcgcagtat agagggtttg cgcgcccttg
      421 tcaccccgtg ttgcgcattc caatcgccag tgtcgcggca atattacgtg aagcacggca
      481 gatattgtca taagccccgc ggaatgcttc gtccaacgta cctatgctgg tcaatacgct
      541 gaagaccgca tcaatgccat gctgatgtac aacgccaaca tcatcggtca ggctacccgc
      601 aatgccaatc accggtttat ggtacttctt cgccacgttt gcgacgccaa tcggtacctt
      661 gccgtgaata ctctggctgt caatacgccc ttcaccggtg atcaccagcg tacaatcgtg
      721 aatatgttcc tccagattca gcgccgtagt gacgatttca ataccacttt tcagttccgc
      781 accaagaaac gccatcagcg ccgcacccat accgcccgca gctcctgcgc cggggacatc
      841 ttttacatca acatgcagcg cttttttaat gacatcggca tagtgagaga ggttattgtc
      901 cagctcaaca atcatcgctt cactggctcc cttttgtggg ccaaagatgc gcgatgcgcc
      961 gttatcgccc accagcggat tggtgacatc acaagcgacg cgaatgacgc aatcttttaa
     1021 gcgcggatcg aggccggaaa tatcaatatc attcagagta ttaagactac cgccgccaaa
     1081 accaatttca ttgccgttgg cgtcgcataa tttcgccccc agcgcctgta ccatgcctgc
     1141 gccgccatca tttgtagcgc taccgccaat gccgataata atgtttgtcg cgccgctctc
     1201 cagcgcctgc aggattaact cgcctgtgcc gcgtgaagtg gtcacgagtg gatcgcgttt
     1261 ttccgcaggt accagctcca gcccactggc cgccgccatt tcaataaacg cggttttgcc
     1321 atcgccggag atcccccaac tggcattcac tttctcgccc agcggccctg taacccaggc
     1381 gtgacgttca gaaccctggg tggctgcaat catcgcttcc accgttcctt cgccaccgtc
     1441 ggcaaccgga atagaaacgt actgtgcatc aggaaaaatt tcccgaaatc ctttttctat
     1501 cgcctgcgca acctcgctgg cagataaact ttctttataa gagtctgggg cgattacgat
     1561 tttcatacct atgcctgtta ccacatgacg ccggagggcg tttctcttat tcggcctgga
     1621 ttccaggccc ggattgcaat acgccatccg ggcacgacgt cattaacgag taacttcgac
     1681 tttcgccagt ttttcgtagt agcacaccag ggcgctatga tccgccgttc ctaaaccatc
     1741 tgctcgcagt gcctgcatca tctccataac cgcagctgtg agcggcagtt gcgcgccgac
     1801 gccgtgggaa gtatccagcg catttgccag atccttaata tgcagatcaa tacggaagcc
     1861 cggcttgaag ttgcggtcca tcaccatcgg cgctttggca tccagcacgg tactgcccgc
     1921 cagtccaccg cgaattgcct gatacaccag gtccgggtta acgcccgctt tagttgccag
     1981 cgttaacgct tctgacatcg cggcaatatt cagcgccaca atgacctgat tcgccagttt
     2041 ggtgacgtta cctgcaccaa ttgccccggt atgcaccacg gaacccgcca ttgctttcat
     2101 caaatcatag tatttgtcga aaatagcctt gtcgccgccc accatcactg acagcgtacc
     2161 gtcgatggct ttcggttcac cgccgctcac cggagcatcc agcatatcaa tgcctttcgc
     2221 tttcagcgct tcgctgattt cacggcttgc cagcggtgcg atagaactca tatcgatcaa
     2281 taccgtacct ggcttcgcgc cttcaataat gccattctca cccagcgcca cctctttcac
     2341 atgaggggag tttggcagca tggttatgat gacgtcgcac tgttcagcga tcgctttagc
     2401 cgtagaggct gtttctgcac ctgcagcaat cacgtcagca atagcttctg ggttacggtc
     2461 agcaaccacc agcgagtaac ctgctttcag aaggttttta ctcattggtt tacccataat
     2521 ccccaggcca ataaaaccaa ctttcatagt catatcaatc atctctcttg ttgcggtggt
     2581 ggttattttt taaaggtatc agccagttta tgagtggcag agcggaagac gccgagatcg
     2641 ctgccgacag ccacaaacgt cgcgccccat tccagataac gacgcgcatc ggcttcgacc
     2701 ggcgcgagga taccgctggg tttgccgtgc gcgctggcac ggttaaaaat gtgctgaatt
     2761 gctttttgta catccgggtg tgatgcattg ccgagatggc ctaatgccgc ggccagatcg
     2821 ctggggccga cgaagatgcc gtctacgcct tcggtagcgg caatggcatc gacgttatct
     2881 acgccctgct gactttctat ctggaccaga atagtgatgt tcttgttcga ctgagcgaaa
     2941 taatccgcca cggtgccaaa catattggcg cggtgagaaa cggagacgcc gcgaatgcct
     3001 tccggtgggt aacgggttga tgccaccgcc tgctctgctt cctcttttgt ttctacaaaa
     3061 ggaatcagga agttatagaa accgatatcc agaagacgct taataatttc cggctcgttg
     3121 gtcggcactc acactactgg cgcgctggcg ctgcctttca aggccattaa ctgcggaata
     3181 aacgtggaga tatcgtttgg cgcatgttcg ccatccagca ccagccagtc aaacccagcc
     3241 aaaccaagaa cttcagtgct aatcgggtta gagagtgctg accagcaacc aatttgtacc
     3301 tgtttcgcag ccagtgcggc tttgaattta ttcgggaaaa catcgttatt catcgcttat
     3361 acctttgctt atttctgcaa ttccatacgt ttaatgtcgc caactacgaa gaggtagcag
     3421 accatcgcca tcagcgctga acatcccacg aaaaccagtg ctgcattgaa ggagtgcagt
     3481 tcacttacca ggtagccaat caccagtgga gtgacaatgg aggcaacatt gccaaagacg
     3541 ttaaagacgc cgccacagag gccaacaatc tctttcggcg cggtgtcaga aatcaccggc
     3601 cagcccagag caccaaatcc tttgccaaaa aaagccagcg ccatcagcat gaccaccagc
     3661 gtggtgttgt tggtgtagtt acataagatg atggtggaag ccagcaacat tcccagcaca
     3721 atcggtagct tacgtgccag ggtcagggat aaaccgcgtt tgatcagata atccgagaag
     3781 acacctccca gcacgccgcc cgcaaaacca cacagtgccg gaatcgaggc gaccagaccc
     3841 actttcagaa tcgacatgcc tttttcctgc accagataaa tcgggaacca ggtgaggaag
     3901 aaccaggtga tggtgttgat aaaatattgt ccgaaaaata cgcccaacat catgcggtta
     3961 gagagcaatt gcttgatgta atgcagtttg ggaccgcttg ctgccgcgct gcccggcttt
     4021 ttgtggtcca tatcgaccac cgcgccattt tcagagataa acttcagctc ttcctcagac
     4081 atacgtgggt gatctgtcgg gttatgaatc aacttgatcc acagcgccgt cagcacaaaa
     4141 caaatcaccc ccataacggt aaagacgtgc tcccatcccc aggcgaaagt cagccagcca
     4201 agcagaggcg aaaagagcgc cagcgagaaa tattgtgccg agttaaagat ggcggaggca
     4261 gtaccacgtt ctttcgtcgg gaaccaggcg gcgacaatac gggcgttcgc cgggaaggat
     4321 ggagcttccg agaatcccag cataaagcgc ataaagaaca tggagatccc tgcccaagcc
     4381 agcgggaaca tatcaacaaa gccttgcagg aaggtgaaca gcgaccagaa aaagaggctg
     4441 taggtgtaaa cttttttcga gccaaactta tcaagcaacc agccgccggg gatttgcatc
     4501 agcaagtagg cccagccaaa agcggagaag atgtaaccca tcgaaaccgc acttaactgc
     4561 aactcttttg ccacttcggt accagcaata gacagcgttg cacgatcggc gtagttaacg
     4621 gcggtaacaa taaaaataat cagtaatatt aaatagcggg tatgcacacc tttccttttt
     4681 tcgtcaacgg tgtccagaat cattttattt acctcgggta cttatgctga tttttattat
     4741 tatggggaag gtgttattta taagtttcat ttatgccgta acgacaatga actcgggaat
     4801 tagtataag
//
LOCUS       DAZPAB010000246         4767 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZPAB010000246 DAZPAB010000000
VERSION     DAZPAB010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.247"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            93..2273
                     /locus_tag="ACHUPG_003777"
     CDS             93..2273
                     /locus_tag="ACHUPG_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR0863183.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVEPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(2414..3697)
                     /locus_tag="ACHUPG_003778"
     CDS             complement(2414..3697)
                     /locus_tag="ACHUPG_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR0863184.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(3832..>4767)
                     /locus_tag="ACHUPG_003779"
     CDS             complement(3832..>4767)
                     /locus_tag="ACHUPG_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0863185.1"
                     /translation="TEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILAS
                     RGIKQKTLINYMSKIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTL
                     SDAFREAIAEGHITTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMEL
                     AVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCK
                     EILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQ
                     ISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         1148 a         1161 c         1216 g         1242 t
ORIGIN      
        1 cttgtcgcct tcctgcaact cgaattattt agagtatatc catttattat ctttctgcgc
       61 acttcacggt gcgcagatat ctggagcatt tgatgatcaa gttatctgaa aaaggcgtgt
      121 ttctcgccag taataacgaa ataattgccg aagaacattt caccggcgaa attaaaaaag
      181 aagaagcaaa aaaaggcact attgcctggt ctattctctc ttctcataat acgtccggaa
      241 atatggataa acttaaaatt aagtttgatt cattagcctc tcacgatatt acctttgttg
      301 gtattgtaca gaccgctaaa gcgtccggaa tggaacgttt cccgctgccg tatgtgctga
      361 ccaactgcca taactcactc tgcgccgtcg gcggcaccat taacggtgat gaccatgttt
      421 ttggtttatc ggcagctcag cgttatggcg gtatttttgt gcctccgcat attgcggtca
      481 tccatcaata tatgcgtgag atgatggctg gcgggggcaa aatgatcctc gggtcagaca
      541 gccacacccg ttacggtgca ttagggacaa tggcagtcgg tgagggtggc ggtgagttgg
      601 taaaacagct gcttaatgac acctgggata tcgactatcc gggcgtggtt gcggtgcatc
      661 tgaccggaaa acctgcgccg tatgtggagc cacaggatgt ggcgctggct atcattggcg
      721 cggtgttcaa aaacggttac gtcaaaaaca aagtcatgga gttcgttgga ccgggcgtta
      781 gcgcgctctc taccgatttc cgtaacagcg ttgacgtgat gaccacggaa accacctgtt
      841 taagctccgt ctggcaaact gatgaagaag tgcataactg gctggcgctg cacggtcgcg
      901 gtcaggatta ctgccagctt aaccctcaac cgatggcgta ctacgatggc tgcatcagcg
      961 ttgatttaag cgccatcaaa ccaatgattg cgctgccgtt ccacccgagc aacgtgtatg
     1021 aaatcgacac actgaaccag aacctgaccg acattctgcg tgagattgaa attgagtccg
     1081 aacgcgtggc gcacggtaaa gccaaactct cgctgctgga taaagtggaa aatggtcgcc
     1141 tgaaagtgca gcaggggatt atcgcgggct gttctggcgg taactacgaa aacgtcatcg
     1201 cggcggcgaa tgcactgcgc ggtcaatcct gtggcaatga caccttctcg ctggcggttt
     1261 acccgtcatc acagccggtg tttatggatc tcgccaaaaa aggtgtggta gcagatttga
     1321 ttggcgcagg cgcaatcatc agaaccgcat tctgtggccc atgctttggc gcgggcgata
     1381 cgccaatcaa caacggtttg agtattcgcc acaccacgcg taacttcccg aaccgcgaag
     1441 gctctaagcc agccaatggg cagatgtcag cggtggcgtt gatggacgct cgttctatcg
     1501 ctgcgactgc ggcaaacggt ggctatttaa cctctgccag cgaacttgat tgctgggaca
     1561 acgtgccgga gtacgccttc gatgtaacgc cgtataaaaa ccgtgtttat cagggctttg
     1621 tgaaaggggc aactcagcaa ccgctgattt acggaccgaa cattaaagac tggccggaat
     1681 tgggtgcgct gactgacaat atcgtcctga aagtgtgctc gaagatcctc gacgaagtca
     1741 ccaccaccga cgaactgatt ccttccggtg aaacctcttc ttatcgttca aatccgattg
     1801 gtctggcggg gaatgtcagc gagctgacag aggtgtttgc gcgcattaag cagattgctg
     1861 gtcaggagca tattgatccg ctgcaaactg aaattggtag catggtctat gcggtgaaac
     1921 caggcgatgg ttctgcgcgt gaacaggcgg cgagctgcca gcgtgtgatt ggcggtctgg
     1981 cgaatattgc cgaggagtac gcgactaaac gctaccgttc taacgtcatc aactggggga
     2041 tgttaccgct gcagatggcg gaagtgccaa cctttgaagt gggggattac atttacatcc
     2101 ctggcattaa agcggcgctg gataatccgg gtacgacgtt taaaggttat gtgatccatg
     2161 aagatgcgcc ggtaacggaa attacgcttt acatggaaag cctgacggct gaagagcgcg
     2221 agattatcaa ggcgggtagt ttgattaact tcaataaaaa ccgtcagatg taaaaagcgc
     2281 catgtgaatg taggtcgcat tcggcactta ttgtcggatg cgatgctggc gcatcttatc
     2341 cgacctacaa atcgcatcga accgtaggcc ggataaggcg tttacgccgc atccggcaaa
     2401 tagttaatag ctcttacttc ttctcctctg caaccacttt gctgcccacg ccgcggttat
     2461 tgtattccca catgcggttg tagttagtgt cattcagatt gcgctgtatt tcgtcgttat
     2521 catctacgct gccggtatta cccgcaaacg gacgattaga gatcaccgca tcggcccacg
     2581 gtttagccgt gttaaaacct tcgttgatgg cgctatcacg gatcaccacc tgaccgttgg
     2641 tattagcatc aacatccagc gagcggccca gttgcgccac gccatcaccg gaagcattga
     2701 aacggctgtt tacggcgagg aaaccgtaat agatgttaga cagcgtagcc ggtgcaaaca
     2761 catatgcttc ttgctgggta cgtgagttca ccacgcggaa ttcggtgtta tcgaacacca
     2821 ctgcgccgcg accagaaacg atatccacat ccccttcaat atagctgttg gtcaccagcg
     2881 tacgcggctg acggttggtt tccagacggt tctgcacacc gctgttggtg acaaagaagg
     2941 tgttctgacg accgagaatg ttaacgttgt taatctgtac ttggtcaccg tcagtacgca
     3001 gtgctaccgc cggatggtta cctgcatcta cgctatcgcc cagcgtgttt tcgatggtca
     3061 gattttgcag ttgcagtcca ttgttttgtg accagaagac cgcagaacac agaacaccga
     3121 tactgtcgct gcgtttgctc tggcagctat cgtacatata ccacgctggt ttacctggca
     3181 tatatttgcc gcgcgggttg acgtcgtgac gccagtcggc agggctcatg cccccatcaa
     3241 gggaaagccc aatcttcaca tcaatcggtt tttcacctgt accgtacaga gtgattccac
     3301 ctggagcagc agggacatac accgttccct gatactcacc aggcatcacg gcaatatact
     3361 ggcgcttgtt ggtgcgcttg ataattgccg catctaccgc cgcctgaatc gtggtatgcg
     3421 ttacaccttg agtacccgcc gggccgacaa caaagtcagg ttgcgcaggc agggttatcg
     3481 gggacggatt ccacgctgca gcacctggtg tcagggatgc aaaatagtgt tgcgcatcga
     3541 aattcagcgc ttcttttgcc gacagaatcg ggcgagaaga ggtaccaggc gcagtttgat
     3601 cagaaggacg ttgatcgggt ggtgttgagc tacaggcggt cagcgtcacg ccaaaagcca
     3661 atgccagcgc cagacgggaa actgaaaatg tgttcacagg ttgctccggg ctatgaaata
     3721 gaaaaatgaa tccgttgaag cctgcttttt tatactaagt tggcattata aaaaagcatt
     3781 gcttatcaat ttgttgcaac gaacaggtca ttatcagtca aaataaaatc attatttgat
     3841 ttcaattttg tcccactccc tgcctctgtc atcacgatac tgtgatgcca tggtgtccga
     3901 cttgtgcccg agaagatgtt gagcaaactt atcgcttatc tgcttctcat agagtcttgc
     3961 agacaaactg cgcaactcgt gaaaggtagg tggatcccct tcgaaggaaa gacctgatgc
     4021 ttttcgtgcg cgcataaaat accttgatac tgtgccggat gaaagcggtt cacgacgagt
     4081 agatgcaatt atggtttctc cgccaagaat ctctttgcat ttatcaagtg tttccttcat
     4141 tgatatcccg agagcatcaa catgcaatgt tgtagggatg gcaattttta cgcctgtttt
     4201 gctttgctcg acataaagat atccatctac gatatcagac cacttcattt cgcataaatc
     4261 accaactcgt tgcccggtaa caacagccag ttccattgca agtctgagcc aacatggtga
     4321 tgattctgct gcttgataaa ttttcaggta ttcgtcagcc gtaagtcttg atctccttac
     4381 ctctgatttt gctgcgcgag tggcagcgac cgggtttgtt gttatatggc cttcagctat
     4441 tgcctctcgg aatgcatcgc tcagtgttga tctgattaac ttggctgatg ccgccttgcc
     4501 ctcgtctatg tatccattga gcattgccgc aatttctttt gtggtgatgt cttcaagtgg
     4561 agcatcaggc agccccctcc ttattgcttt aattttgctc atgtaattta tgagtgtctt
     4621 ctgcttgatt cctctgctgg cgaggatttt ttcgtagcga tcaagccatg aatgtaacgt
     4681 aacagaatta tcactgttga ttctcgctgt cagaggcttg tgtttgtgtc ctgaaaataa
     4741 ctcaatgttg gcctgtatag cttcagt
//
LOCUS       DAZPAB010000247         4740 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZPAB010000247 DAZPAB010000000
VERSION     DAZPAB010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.248"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..746
                     /locus_tag="ACHUPG_003780"
     CDS             <1..746
                     /locus_tag="ACHUPG_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0863186.1"
                     /translation="VDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWN
                     NVTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIAT
                     LGSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKL
                     SFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPL
                     GLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1080..3359
                     /gene="ydeP"
                     /locus_tag="ACHUPG_003781"
     CDS             1080..3359
                     /gene="ydeP"
                     /locus_tag="ACHUPG_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR0863187.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3607..3810
                     /gene="safA"
                     /locus_tag="ACHUPG_003782"
     CDS             3607..3810
                     /gene="safA"
                     /locus_tag="ACHUPG_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR0863188.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3878..4639
                     /gene="ydeO"
                     /locus_tag="ACHUPG_003783"
     CDS             3878..4639
                     /gene="ydeO"
                     /locus_tag="ACHUPG_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR0863189.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
BASE COUNT         1338 a         1026 c         1086 g         1290 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgcgcacaat tgaagccaga gtcttatttt ttatattgga aaatatgtta atttaaacac
      901 tatgagagat gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa
      961 aatagaaaga gagtagtccc ttactcttat atatccatgt tggcgataat ccctttgtat
     1021 gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga
     1081 tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg
     1141 tagcgaatgc agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca
     1201 tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct aagaacagtg
     1261 cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ttgggaagtc acggataagc
     1321 aggtaaatgc ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc
     1381 acgagcttga ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact
     1441 gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct
     1501 atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct
     1561 ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca
     1621 tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg
     1681 tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta
     1741 caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga
     1801 tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt
     1861 ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg
     1921 gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg
     1981 caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg
     2041 gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt
     2101 ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca
     2161 ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc
     2221 aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc
     2281 cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt
     2341 ctgcagagtt tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg
     2401 gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct
     2461 gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa
     2521 cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg
     2581 cacggcatag ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg
     2641 gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt
     2701 taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg
     2761 cagcactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc
     2821 gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc
     2881 ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta
     2941 aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta
     3001 agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg
     3061 atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag
     3121 ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg
     3181 gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc
     3241 gctcactggt gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc
     3301 cattaagtgg tattcctggc tataaaagta ttccggttga attagaacca tcaaattaat
     3361 gcctcttctc atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta
     3421 ttgcgcaatt tgctcacatg gattaaatta aactacatac tataagatat aaacttctgc
     3481 ctacagctgt aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc
     3541 agcctgttat attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata
     3601 tttgaaatgc atgcgaccac agtgaaaaac aaaatcacgc aaagagataa ctataaagaa
     3661 atcatgtctg taattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg
     3721 gcaattgatc agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc
     3781 attatcaata gtttgcaaga ctggaaataa acagcaaatt ataaatgtga aatgtttaaa
     3841 aggtatcgat aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat
     3901 atttattcat catgctttca acgctaacat tttagataaa gattacgcct tctccgacgg
     3961 cgagatcctg atggtagata acgctgttcg tacacatttt gaaccttatg agcggcattt
     4021 taaagaaatc ggatttaatg aaaataccat taaaaaatat ctacaatgca ctaacatcca
     4081 gacagtgacg gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt
     4141 gcttaatgaa atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga
     4201 acttttgctt ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt
     4261 aactaacggt gtgctatccg tttccgggaa agtgagaaat attgtcaaca tgaagctggc
     4321 gcacccatgg aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa
     4381 gaaaaaacta aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca
     4441 gcacgcaaaa aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg
     4501 ttatgccagt acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa
     4561 gagagtttct aaggagtacc gttgtcaaag gcacacgggt atgaatacgg gcaacacgat
     4621 gagtgcttta gctatttgat tatttgctaa cgagtagtca accacgcacg ctacgtaaga
     4681 attatatggg gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat
//
LOCUS       DAZPAB010000248         4602 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZPAB010000248 DAZPAB010000000
VERSION     DAZPAB010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.249"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(78..866)
                     /gene="phoH"
                     /locus_tag="ACHUPG_003784"
     CDS             complement(78..866)
                     /gene="phoH"
                     /locus_tag="ACHUPG_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR0863190.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="ACHUPG_003785"
     CDS             complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="ACHUPG_003785"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR0863191.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="ACHUPG_003786"
     CDS             complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="ACHUPG_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR0863192.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3929..>4602)
                     /gene="efeU"
                     /locus_tag="ACHUPG_003787"
     CDS             complement(3929..>4602)
                     /gene="efeU"
                     /locus_tag="ACHUPG_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR0863193.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1081 a         1296 c         1109 g         1116 t
ORIGIN      
        1 ttcagaaaaa gcgctagtcc gggcataaac acccaaaaca aagcccggtt cgcccgggct
       61 ctgcaccgat aatacactta actgtatgca tgcagcgtac gttggcaaag tgccgaacgt
      121 acgcaatcct cttttccgaa ccggacgatc ccaaccattt catcttcttc gaaacgttcc
      181 agcgcgtcac ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg
      241 ttgacgataa ccgtcacgtt ctccccgagg cgggttaaaa acattttcat ttgcgcggca
      301 gtcacattct gcgcctcgtc aagaatgaca actgcatttt caaaggtacg tccacgcata
      361 taggcgaacg gcgcggtttc caccttccct atttccggtc gcaggcagta ctgcataaag
      421 gaagccccta agcgccggac cagcaagtcg tagaccgggc gaaaataggg agcaaacttt
      481 tctgcgatat caccaggtaa gaagccaaga tcttcatcgg cttgcagaac tggacgggtg
      541 acgataatcc tgtcgacatc cttatgtatc agggcctctg ccgcttttgc tgcgctgatc
      601 caggtttttc cgcacccggc ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc
      661 tgcagatagt gcaattgcgc ttcatttcgc gcaaggatgg gcgaggtatc gcgactgtcg
      721 cgggccatac caatggcttc tacgccgctc atctgcacaa gcgaggtgac cgattcttct
      781 tcacgctgct tatggctgcg cgaatcccgt ctcagcacac gttttgcctc gcgacgagct
      841 ttgatcactg ctttttgtct tcccatggag agcaccttga gttgtttgta ttcatcacac
      901 gcgccgttgg cagcgcgatt atgcgcacga acatcagagg gttggcttcc ttgtaagcca
      961 tagtttgctt ttggataaaa tgccgaaaaa cggctacgca caccgtttgc ggcgtcggta
     1021 acacgagtaa agaaaggaag tgagaaaaat acagagtgac gaagttgcta ccggaggaga
     1081 aatctccgcg agtggtggag cgttgcttaa caaaacattg tccagaacag gacgttacca
     1141 tccgcgatct ccatagtgac tgactatcac tgccgggaac ttccgctgct acttaataag
     1201 tacaacagaa ctcgcattca ttgcaacaat atattcactt atatttaacg ataacaccca
     1261 tttcggtgac attaatttct actggaaaga tgacagagtg atgacagtga tggaaaaagc
     1321 tgtgtgcttt ccgcaggatt cgcagggctg gtaaaatgtg cctcgtgttt gccggattcg
     1381 gcgcgaacgc cttacccgac ctacaagtga ttaataattt gtgcacaaaa ctcatgcccg
     1441 ataaacctgc gcatcgggca tatttgcgtc gttcaaacct gcaatagcgc ccttcccaga
     1501 taatcgttcg cgtccttcac tcccggcagc gaaaaaaaat aaccgccgcc gataggttta
     1561 acgtattcct ccagcgcttc gccattgagc cttttttgta ctgtcaggaa gcctttttcc
     1621 agatcgtgtt ggtagcagac aaacagcaac cccatatcca gttgtccgga gttggtgacc
     1681 cccaacgaat aactgtagcc gcgacgcagc atcaggctgg actcactctc cgccgtgcgg
     1741 ggattcgcca gccggatatg gctgtccagc gcgatcacct tcccttccgg gtcgctggcg
     1801 taatcaggca catcatgctc atgctgcatt cccagcggcg caccggtttg cttatcacgg
     1861 ccaaaaatcg tctgctgttc tttcagcggc gttctgtccc aaaattccac tcgaaactga
     1921 atcaagcgta ctgcctgata gctgccaccg attgtccacg caggctcctg ctgatctgcc
     1981 gttacccaca ccactttttg catcaactta tcattctggc tatcgggatt ggcagtgccg
     2041 tctttgaaac ccagcaaatt aatcggcgtc tctttgcctt tactacgcgc cgcgtgatcg
     2101 gaaataaacc cttcccgctt ccagcgcaca ctgagcaaat ccggcgtgtg tttgatgata
     2161 tcgcgcagcg catggttcac cgtgtcctgg gtgttggcgc aaatctgtag caacacatca
     2221 ccatgacata acgccgcatc cagcgagtcg ttggggaaac gcgtcatctt ctgcagcttt
     2281 tttggcatct gtggcgcaag gccaaagcgc tcatcaaaca atgagtgacc caccgataac
     2341 gtgatggtga gattatcggg cgcaatgtag ccgccaagaa tgccggaatc gagtggtggc
     2401 aggcgcggat ttggcgtttc tggtgctgct ccgccctgac tcagaaaagc aaaacgctga
     2461 gtcaacaagc gaaacaaccg ctcaagatcg gctttatcgc tggcaagcac atcaaacgcc
     2521 accagcatca ttgcggcctg ttgtggcgtc aggatccctg cctgatgctc accataaaac
     2581 ggctgtttct cattgcgtgc atccggtgaa agcgtgcccg gtgcactttg cgttttttgt
     2641 gcatgagcga ccggacaact tcccgccagc gcaccaatcc ctttcagtaa acgtcggcgt
     2701 gacggttcat tcacgccgtt ttcatcttta tactgcataa cgcttaatcc agtcccagca
     2761 caccgcgaag ttgcgccaga tcttccgcca gcgcagtaat cggccctttc agcgcattcc
     2821 ggtcggcatc ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca
     2881 gaatggtatc gaccttttta aagttggcat cgacttttgc caacagttcc gggttggctt
     2941 tttgcagttg tggacgcagc agatcgacaa ttttctgcga gccttcaacg ttagcctgga
     3001 aatcccacag atcggtgtgg ctgtagcgat cttcttcacc gctgattttg ctggctgcca
     3061 cttcctcaat cagtccggct gcgccgccga ccacttttga aggtgggaaa gccagttcac
     3121 tgatgcgttt ttgcaaatcg accacatcgg tataaagctg ctcagcgtac tgatccatcc
     3181 ctttggtggt gttgtcgcca aacaatgctt tttccagacg gtggaaacca gtgaattttg
     3241 ggtcggcggc tttttgctcg taatcatctt cacgggcgtc aatgctgcca tccagatcgg
     3301 agaacagttc agcaatcggt tcaatacgct catagtgctg gcgcgtcggc gcatacagtg
     3361 ctttcgcttt ttcgatatcg cctgctttaa tcgcgtcggt aaaggctttg gtgtcggtca
     3421 ccagctgcgt ggtttccgcc atgacatacg ctttatatgc agtaattgca ccaccaagac
     3481 ttaacagcgc atcactttgc gccgcatccg ccgttgcctc acctttgacg atcaacttcc
     3541 ctttcgggtt agtcagcaga ccgcaggtca tatcgtattc gccaggctgt aaattcgccg
     3601 tcattttctg gctaaaacca ggggcgatat tttcccgctc ttccaccacc atcacgcctt
     3661 tgaggatctc ccactccagc gccttctggc tatggttctg aataatgaac tgtgttttcc
     3721 cggcgttaac cgtaatggtc atcggttcgc actgcttatc cgtcacggtc actttgacct
     3781 gcggcacatc agcggcgtta gccataaaag cagaagaaaa cagcgcagcc acgctcaact
     3841 gcaatgcgtt acggcggaag ttaatggtca tgacgagact atccctttaa agtatgttgt
     3901 aactaagcaa gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc gcccctgcgc
     3961 gtggtggcag agcaaatgcc accagcgccg ggatgagata aataaaccag acggcgactt
     4021 cgctgacgct cggtgcttcc tgatagccaa aaatcccttc catcagcgtg ccaaacagcg
     4081 agtgagttga gaggaccgcg ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc
     4141 cggcttcatg aaatgcgcga atggcaccag ccgccagccc tgcggcgacg aagagaataa
     4201 acaggctggt ccatttaaaa aatgcaccaa gattgaggcg aataccgccc cagtagagca
     4261 ggaagccgag caccacggca gtagcaagac cgagcattgc acccagcggc ggccagatcc
     4321 cgacatcttg ttgaaatgcc gccagcagga aaaagaccga ctccagccct tcccttgcaa
     4381 cggcaaaaaa gaccatcatc accagcgccc agccatgatg atttccacgc tgcaatgcgc
     4441 tatcgactgc ctgttccagt tgcactttga cgttgcgcga cactttgcgc atccagaaaa
     4501 ccatccaggt aaggatcacc acggcgatca ccgccacgat accttcaaac agttcctgct
     4561 ctttttgcgg aaattcgccg gtggtttcgt taatgaagat cc
//
LOCUS       DAZPAB010000249         4540 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZPAB010000249 DAZPAB010000000
VERSION     DAZPAB010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.250"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACHUPG_003788"
     CDS             complement(<1..890)
                     /gene="yafC"
                     /locus_tag="ACHUPG_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR0863194.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="ACHUPG_003789"
     CDS             1131..1931
                     /locus_tag="ACHUPG_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR0863195.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="ACHUPG_003790"
     CDS             2009..2779
                     /locus_tag="ACHUPG_003790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0863196.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="ACHUPG_003791"
     CDS             complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="ACHUPG_003791"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR0863197.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4245..>4540)
                     /locus_tag="ACHUPG_003792"
     CDS             complement(4245..>4540)
                     /locus_tag="ACHUPG_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HFR0863198.1"
                     /translation="LPDDTLVCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAK
                     NQITLPVILKNERQINVFLRTEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
BASE COUNT         1110 a         1129 c         1085 g         1216 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcac caatcgtcaa actttggcct ggcttcagct tagatccgcg
     3061 cagtttgttc cactgctgca aatctttggt gcttacgccg agacgtgaag cgatgcttga
     3121 aagcgtgtcg ccagagcgta cggtgtaaac acggctgtta agcggcgtat tgttggcaac
     3181 cagcgtcgac tgtacagcag caatttcgcc tgaagcgaga gattcacgca gttgatctgc
     3241 atgcttcttt ggcaccatca cgtactgcgg cccacttgcg cccagcgtgg agcctttcac
     3301 gccagcgttg aatgtcttca gcttgctgac ggaaatcccc gccatatctg caaccttcgc
     3361 catttcaacc gggctactca ggtgcacacg cgccagagca cggctttcat cggtcgttgg
     3421 cagacgtacg ccataacgct tgctgttttt gagaatatca ctcaatgcca gcattttagg
     3481 cacgtactgc ttcgtttcct gcggcaacgg taacgaccag aagtccgtgg atttcccacg
     3541 cgctttgttc gttttaattg ccttcatgac ccgaccttcg ccgctgttat aagccgctac
     3601 ggtcagaagc cagtcgtcgt caaacatttt gttcagacgc tgcatcatat tcagcgcggc
     3661 agttgttgaa gcaacaacat cgcgacgcgc gtcataattg cgggtctgtt tcaaaccata
     3721 attgcgcccc gtgctcggaa tgatctgcca gatgcctgcg gcattggcgc cagacgttgc
     3781 gtgaggatca aaagcgctct ccactatggg tagtagtacc agttccatag gcatgttacg
     3841 ttttttaact tgccctgcta tccagtacat atacggctct gcccgtaaag ttacatcgtg
     3901 gagatagctc ttattgcgta aatatttctg tttctgttcg cgaatccggt cattttccgg
     3961 aattcccatc tttagctcgt cgccaatgaa agcccacaag tcaccatctg gcgcgataga
     4021 cgtcccatcg tccatccatc gtgcctgact tgtaaacttt gctgcttccc cttgaccagc
     4081 tgcagaaagg ctctgtgcgt gctgttgaac gttgccggta ctctggcaac ccacgagcag
     4141 gacagaggcg agtaatatcg cttttgcctt catgtgtgtg tcaatagttg cttaaaagac
     4201 gaccgatcat aacggcgaac ggagccgatg acaagaaagt tttatcagaa cctatctttc
     4261 tttgacctta accatgcaaa acgctcttca ggttgttgca ataatgtttc ttcattaatt
     4321 ccattaatta aatcaatatc ttccgttctt aaaaaaacat taatttgccg ctcatttttc
     4381 agaattacgg gtagtgttat ttgatttttt gcccgtaact ccttaacttt acgataataa
     4441 tcatttatgg acaaatcgtg cggaaaaata ctcaaagcaa acttcatatt tgataacgta
     4501 tattcatgag cacaacatac caatgtatcg tcaggtaacg
//
LOCUS       DAZPAB010000250         4464 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZPAB010000250 DAZPAB010000000
VERSION     DAZPAB010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.251"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            179..1036
                     /gene="focA"
                     /locus_tag="ACHUPG_003793"
     CDS             179..1036
                     /gene="focA"
                     /locus_tag="ACHUPG_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR0863199.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1091..3373
                     /gene="pflB"
                     /locus_tag="ACHUPG_003794"
     CDS             1091..3373
                     /gene="pflB"
                     /locus_tag="ACHUPG_003794"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR0863200.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3565..4305
                     /gene="pflA"
                     /locus_tag="ACHUPG_003795"
     CDS             3565..4305
                     /gene="pflA"
                     /locus_tag="ACHUPG_003795"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR0863201.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4387..>4464)
                     /locus_tag="ACHUPG_003796"
     CDS             complement(4387..>4464)
                     /locus_tag="ACHUPG_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863202.1"
                     /translation="FDGEELHASYYQSLLSQVRDMVDAL"
BASE COUNT         1135 a         1102 c         1081 g         1146 t
ORIGIN      
        1 tcatttgtta atttttaaaa tttattttta tttggataat caaatagtta ctccgtattt
       61 gcataaaaac catgcgagtt acgggcctat aagccaggcg agatatgatc tatatcaatt
      121 tctcatctat aatgctttgt tagtatctcg tcgccgactt aataaagaga gagttagtgt
      181 gaaagctgac aacccttttg atcttttact tcctgctgca atggccaaag tggccgaaga
      241 ggcgggtgtc tataaagcaa cgaaacatcc gcttaagact ttctatctgg cgattaccgc
      301 cggtgttttc atctcaatcg cattcgtctt ctatatcaca gcaaccactg gcacaggcac
      361 aatgcccttc ggcatggcaa aactggttgg cggcatttgc ttctctctgg ggctgattct
      421 ttgtgttgtc tgcggagccg atctctttac ttccacagtg ttgattgttg ttgctaaggc
      481 gagtgggcgc atcacctggt gtcagttggc taaaaactgg ctaaatgtct attttggcaa
      541 cctggtcggc gcactgctgt ttgtactttt aatgtggctt tccggcgagt atatgaccgc
      601 aaatggtcaa tggggactaa acgtcctaca aaccgccgac cacaaagtgc accatacttt
      661 tattgaggcc gtctgtcttg gtatcctggc aaacctgatg gtatgtctgg cagtatggat
      721 gagttattct ggccgcagcc tgatggacaa agcgttcatt atggtgctgc cggtcgcgat
      781 gtttgttgcc agcggttttg agcacagtat cgcaaacatg tttatgatcc cgatgggtat
      841 tgtaatccgc gacttcgcat ctccggaatt ctggaccgct gtcggttctg caccggaaaa
      901 tttttctcac ctgaccgtga tgaacttcat cactgataac ctgatcccgg ttacgatcgg
      961 taatattatc ggcggtggtt tgttggttgg gttgacatac tgggtcattt acctgcgtga
     1021 aaacgatcac cattaatggt tgtcgaagta cgcagtaaat aaaaaatcca cttaagaagg
     1081 taggtgttac atgtccgagc ttaatgaaaa gttagccaca gcctgggaag gttttaccaa
     1141 aggtgactgg cagaatgaag taaacgtccg tgacttcatt cagaaaaact acactccgta
     1201 cgagggtgac gagtccttcc tggctggcgc tactgaagcg accaccaccc tgtgggacaa
     1261 agtaatggaa ggtgttaaac tggaaaaccg cactcacgcg ccagttgatt ttgacaccgc
     1321 tgttgcttcc accatcacct ctcacgacgc tggctacatc aataaagcgt tggaaaaagt
     1381 tgttggtctg cagactgaag ctccgctgaa acgtgctctt atcccgttcg gtggtatcaa
     1441 aatgatcgaa ggttcctgca aagcgtacaa ccgcgaactg gacccgatga tcaaaaaaat
     1501 cttcactgaa taccgtaaaa ctcacaacca gggcgtgttc gacgtttaca ctccggacat
     1561 cctgcgttgc cgtaaatccg gtgttctgac cggtctgcca gatgcttatg gccgtggccg
     1621 tatcatcggt gactaccgtc gcgttgcgct gtacggtatc gactacctga tgaaagacaa
     1681 atacgctcag ttcacctctc tacaggctga tctggaaaac ggcgtaaacc tggaacagac
     1741 tatccgtctg cgcgaagaaa tcgctgaaca gcaccgcgct ctgggtcaga tgaaagaaat
     1801 ggctgcgaaa tacggctacg acatctctgg tccggctacc aacgctcagg aagctatcca
     1861 gtggacttac ttcggctacc tggctgctgt taagtctcag aacggtgctg caatgtcctt
     1921 cggtcgtacc tccaccttcc tggatgtgta catcgaacgt gacctgaaag ctggcaagat
     1981 caccgaacaa gaagcgcagg aaatggttga ccacctggtc atgaaactgc gtatggttcg
     2041 cttcctgcgt actccggaat acgatgaact gttctctggc gacccaatct gggcaaccga
     2101 atctatcggt ggtatgggcc tcgatggtcg taccctggtt accaaaaaca gcttccgttt
     2161 cctgaacacc ctgtacacta tggggccgtc tccggaaccg aacatgacca ttctgtggtc
     2221 tgaaaaactg ccgctgaact tcaagaaatt cgccgctaaa gtgtccatcg acacctcttc
     2281 tctgcagtat gagaacgatg acctgatgcg tccggacttc aacaacgatg actacgctat
     2341 cgcttgctgc gtaagcccga tgatcgttgg taaacaaatg cagttcttcg gtgcgcgtgc
     2401 aaacctggcg aaaaccatgc tgtacgcaat caacggcggc gttgacgaaa aactgaaaat
     2461 gcaggttggt ccgaagtctg aaccgatcaa aggcgatctc ctgagctacg atgaagtgat
     2521 ggagcgcatg gatcacttca tggactggct ggctaaacag tacatcactg cactgaacat
     2581 catccactac atgcacgaca agtacagcta cgaagcctct ctgatggcgc tgcacgaccg
     2641 tgacgttatc cgcaccatgg cgtgtggtat cgctggtctg tccgttgctg ctgactccct
     2701 gtctgcaatc aaatatgcga aagttaaacc gattcgtgac gaagacggtc tggctatcga
     2761 cttcgaaatc gaaggcgaat acccgcagtt tggtaacaat gatccgcgtg tagatgacct
     2821 ggctgttgac ctggtagaac gtttcatgaa gaaaattcag aaactgcaca cctaccgtga
     2881 cgctatcccg actcagtctg ttctgaccat cacttctaac gttgtgtatg gtaagaaaac
     2941 tggtaacacc ccagacggtc gtcgtgctgg cgcgccgttc ggaccgggtg ctaacccgat
     3001 gcacggtcgt gaccagaaag gtgctgtagc gtctctgact tccgttgcta aactgccgtt
     3061 tgcttacgct aaagatggta tctcctacac cttctctatc gttccgaacg cactgggtaa
     3121 agacgacgaa gttcgtaaga ccaacctggc tggtctgatg gatggttact tccaccacga
     3181 agcatccatc gaaggtggtc agcacctgaa cgttaacgtg atgaaccgtg aaatgctgct
     3241 cgacgcgatg gaaaacccgg aaaaatatcc gcagctgacc atccgtgtat ctggctacgc
     3301 agtacgtttc aactcgctga ctaaagaaca gcagcaggac gttattactc gtaccttcac
     3361 tcaatctatg taattagatt tgactgaaat cgtacagtaa aaagcgtaca ataaaggctc
     3421 cacgaaagtg gggccttttt tagcacgaga gccttttttg tcagctatct atactttaag
     3481 gtgactgcca aaacagactc gacgtagcct tcgagctgcg caccaacacg gcctcagatg
     3541 ggccacatct ggagaaacac cgcaatgtca gttattggtc gcattcactc ctttgaatcc
     3601 tgtggaaccg tagacggccc aggtattcgc tttatcacct ttttccaggg ctgcctgatg
     3661 cgctgcctgt attgtcataa ccgcgacacc tgggacacgc atggcggtaa agaagttacc
     3721 gttgaagatt tgatgaagga agtggtgacc tatcgccact ttatgaacgc ttccggcggc
     3781 ggcgttaccg catccggcgg tgaagcaatc ctgcaagctg agtttgttcg tgactggttc
     3841 cgcgcctgta aaaaagaagg cattcatacc tgtctggata ccaacggttt tgttcgtcgt
     3901 tacgatccgg tgattgatga actgctggaa gtaaccgacc tggtaatgct cgatctcaaa
     3961 cagatgaacg acgagatcca ccaaaatctg gttggagttt ccaaccaccg cacgctggag
     4021 ttcgctaaat atctggcgaa caaaaatgtg aaggtgtgga tccgctacgt tgttgtccca
     4081 ggctggtctg acgatgacga ttcggcacat cgcctcggtg aatttacccg tgatatgggt
     4141 aacgttgaga aaatcgaact tctcccctac cacgaactgg gcaaacacaa atgggtggcg
     4201 atgggtgaag aatacaaact cgatggtgtt aaaccaccga agaaagagac catggaacgc
     4261 gtgaaaggca ttcttgagca gtacggtcat aaggtaatgt tctaatttcc tcgtaaagcg
     4321 gcaactttat tgagttgccg cttttttatt ccgcgcttca ttctgctaca gcgtttttaa
     4381 cacatcttat agtgcatcta ccatatcccg tacctgagat aataacgact gataatagct
     4441 cgcgtgaagt tcttccccat cgaa
//
LOCUS       DAZPAB010000251         4431 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZPAB010000251 DAZPAB010000000
VERSION     DAZPAB010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.252"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..195)
                     /locus_tag="ACHUPG_003797"
     CDS             complement(<1..195)
                     /locus_tag="ACHUPG_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863203.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSL"
     gene            complement(252..2612)
                     /locus_tag="ACHUPG_003798"
     CDS             complement(252..2612)
                     /locus_tag="ACHUPG_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR0863204.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(2650..4335)
                     /locus_tag="ACHUPG_003799"
                     /pseudo
     CDS             complement(2650..4335)
                     /locus_tag="ACHUPG_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
BASE COUNT         1215 a          980 c          976 g         1260 t
ORIGIN      
        1 caatgaaccg gtatgaattt gcagccataa atttacctga tcctttggat gagcatgcgg
       61 ataaatcata tatcgcaagt cattgtccag ttgcccggta attaactttt catcctgcgg
      121 taaggcggcg gcaatcagcc gcccggggag caacagagtt gccactaacg tcagtaagaa
      181 acagaggttt ctcataatta tctccatgcg aaaaccgggc gaatttaccc ggttaagtaa
      241 aatccgaact attaaaattt catgctgaca tcaagccaga aagtacggcc actggcgtag
      301 gtcgccacac cggtgttcgt tgtatcaaca gcggttttgc tatccagtac attgagaata
      361 tcggcactga tcgtcaggtt ttgttgtttc agaaattgtg gcgtccagga caagcgggtg
      421 tcccaggtca ggctgctatc cagtttttcg tcaacatact gcttgtaatc tgaatattcg
      481 ctgatgtatt gcgcatttgt cttaccaagg ataatgcgag ctttacgcgc ttcttgccag
      541 gccagcgtat tcgcccacac taacccgctc ggttgatgcg tgaaatccat gtttaaggag
      601 atctttaatg ggttattaaa atctgccact ggaacgctat cgtaagagac cagattaccg
      661 ttataaacca cctggttatc accggtattg ctctcctcat aaccattgtt taacgacaag
      721 ttgcccttgc tcttgatata gctaaagaca atttgcgggt taatatctac ctggcggata
      781 tgcaggggtt cggccagttc aaaactgagg ttaaacgaat gcgttttggt tttgccatcg
      841 ttgttatatt cagtaatggt ggttttagtc gcgctgtcgg tacgactgct tttgctgatt
      901 tgatcatgcg cttcacttgc gcgtgcaata acgttcttat cgattttttg ctgcaatccc
      961 attgccagtt catcgttata aggcgttttc aaattctgat aacgcgtcag ggttttatta
     1021 cctgataccg attccgtcca gctattgcgg atatcacgta atcccatatc aagaatattc
     1081 ccgccatagt aacggttata accggcggta atcattgagg tttgatcagc aaaaatatcc
     1141 cattccgtca taaagcgcgg ggagatattg tggtttgaca gatagttgtc atagtcataa
     1201 cgcacaccgg gcattaacga cacattccgc cagctaatgt gatccgccat atacagcgtg
     1261 tagttgtcaa ttcccaggct gcctttacct ttatggtaaa tggtatggtt agttttcttt
     1321 cctgcagcat taatcacata ggattcagac tggttatggc gttcagtcca tgcatcggaa
     1381 tagatgtatt ccgcgccgaa gtagggttga tgcgaaacat cacccacggc gaatttttgc
     1441 cagtccaggc gtgttttgaa ggtgtaatta tctacagcct gggaaatgtg tcctaatccg
     1501 ccacgggtac aacgccctgt aatatcacca tatgtacatg aaagttcggt gtaccagatg
     1561 tcatgatcgt gacgggtata atcactaata tgatcccaac caacggtggt acgtagtttg
     1621 gcccatgcga gttgcgtatc catatcccat gccagaccat aggatttatt acccatttcg
     1681 cgatcagact gcgggaaggt gctggtatta taatcacggc tggagccggt atattttaag
     1741 gttaaatcgt gggtaaagcg gtcgctggca aaccaggtaa atttgctcaa tgcagtatcg
     1801 ataacgtttt tatactgtgc ccgaccggcg acaatgccgt cattcgaaac ataatccgcg
     1861 cgggtgatat cagactggcg gcgcgataaa ccggcggtaa cgccaaagtt atcagccagc
     1921 tcctgattaa acgacaaggt ataaaagttc tttttaaaat ctggagagaa ataggtactt
     1981 cctgaagaac cttgattaaa tgcgctcttg ttattctcat cgatatgcga tgttaaccag
     2041 tccaaacgcg tagtgcgata acccaatttc accttgctat catcagcgtt gaagcgtttg
     2101 atctttgcat caattacccc gccattgaag cgaccaaatt caaccggcac aaaactgtca
     2161 taaagcgtca cattgtccag taagctgaca tcaagataat acccctgtga catcccgcta
     2221 atattggttg cactactggc atcggactca ttcgctgggt tcaggttatt agttgcacta
     2281 ataccgtcaa tcaaataggc attctggtag ggcgacgcac cgtgaataga gattttctca
     2341 gggcgaatat ctccctggtt caacgaggta ctttgcgttg aatccatgcg taccgcaggg
     2401 ttggttctca gcagatcgct gatattgcca ttcccggtcg gtaaacgctc gatgctttca
     2461 ctggtgtaat gcgtgttgcc gtttacgggt acagggacag gagcatagac cgtcatctct
     2521 tcaaaaaaat acatgtacat gtttttgtca tcgacggcct gcgccacatc agcgccacat
     2581 aaaatgacgc caggaataag aactcgcttc atttatttat atctccgtac agcagggctt
     2641 ttattgttgt tataaaaccg tgctaatgtc acaaatatca tcagccaggt tccagacacc
     2701 gggttgatgt gtaaccataa tgacgccgct tgtgggtagt ttttcacgca ctaaacgcag
     2761 taagcgtata gcctcttgtt cctcaagatg agaggtagtt tcgtcaagaa atatccattt
     2821 cggacgtcgt aaaattaatc gtgccagggc gatacgttgt ttttcgccgc tggaaagaat
     2881 atctccccag cgatcgtggt catgaatacg cgcagccaat tttcccagac caacctgatg
     2941 cagtacttcg ctcaacgatt tatcgtctac gggcagggga agtgctttac aaataatctc
     3001 tttcagtaag ccgcttttga ttaacggtgt ttgtgacaca taccaactgt cagcaggaga
     3061 agaaatatca cctttaaacc acggccagca gtgggataat gttttaagca gtgtggtttt
     3121 tcccgcacca gagtagcctt tcagtaatag ccatttgcct ggcgaaacat gaaagttcaa
     3181 gttctctaat atgatcttat tatcaggcgt acgaatactc gcattagcca cttgtaccgc
     3241 atgttggcaa tttttaggct tattcgtagg gcgctattca gtgagttgat ggaactcata
     3301 caagcgatcg ataaccgcag ccagttcagc aagttcgtca tatttataaa taaaccagct
     3361 taaattgttc gatactagca taaatgcctg gcgcgatttc atcagtccgc ccagattgat
     3421 ctgcccgcta ataaactgcg gcaataacag aaagtaggga agaacgctaa gcgagcgcga
     3481 ataaatattt tgccagtaat caagccaccg ctgacgattc attaaacgat gccagttctc
     3541 tttaatggta tgaaaattgt cgcgcaactc ctggcgttgt agagactcag cattgcttag
     3601 cgcaatcagt tctgcctgct tattatgctg cacaagatta gttcgaaatg tcgcttcgct
     3661 acgttgtttt tccacattaa gcggacgaat acgtttaccc accttatggg taaataaagt
     3721 tccaccgatc acaatgagca cgacggtata gaccatatac ccctggatat tccattctgt
     3781 tccaccaaca gtgaatgaga gcgtacccgc gctttgccaa agaataacgg taaaggtgat
     3841 cagcatgcta agtgactgga tgaagccaaa tgaaagactg agcgttttgt tgatcagtaa
     3901 gagaatgtct tcagcgatac gttggtcagg gttatctgta tttttatgct cgccgtagat
     3961 ctgcgtgaag tagtagtttt tatctgcaaa ccagcgatta aggtagtaat ctgttaacca
     4021 ttcgcgccag cgaatagtca gtagtttaat taaccatgtt ttgtttgcgg atattaatac
     4081 aaagatcccc agcagggcag ggaaccaaag aacgagctgc caaagcttat cggtttcttt
     4141 ctggcttagc gcattgaaaa aatcattatt ccagtcgttt aaccagacct gaattttaac
     4201 cacgccaagg atcattgcga ggataataat gattaacaac actgaagttt tattattctt
     4261 acgcagccaa aagggcttca gcagacagag atactttgct atcaacatac aaaacgtaat
     4321 gggaatggtt atcattagcg aaaattgatt gtgccatcca atgattatct atggcaatga
     4381 tttactgatg aaatttattt aagcatcatt taacaatatg aaatatatag a
//
LOCUS       DAZPAB010000252         4392 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZPAB010000252 DAZPAB010000000
VERSION     DAZPAB010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.253"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(277..1023)
                     /gene="cutC"
                     /locus_tag="ACHUPG_003800"
     CDS             complement(277..1023)
                     /gene="cutC"
                     /locus_tag="ACHUPG_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR0863205.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(1037..1603)
                     /locus_tag="ACHUPG_003801"
     CDS             complement(1037..1603)
                     /locus_tag="ACHUPG_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0863206.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1819..3552
                     /gene="argS"
                     /locus_tag="ACHUPG_003802"
     CDS             1819..3552
                     /gene="argS"
                     /locus_tag="ACHUPG_003802"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR0863207.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3594..3638)
                     /locus_tag="ACHUPG_003803"
     CDS             complement(3594..3638)
                     /locus_tag="ACHUPG_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863208.1"
                     /translation="MSVFRIHLDGNKKA"
     gene            3729..4217
                     /locus_tag="ACHUPG_003804"
     CDS             3729..4217
                     /locus_tag="ACHUPG_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFR0863209.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         1174 a         1074 c         1053 g         1091 t
ORIGIN      
        1 atatgattta ttacaatgta atcattaatt gctaaggaat aacccagttg ctatttagaa
       61 tatatattta tcagttttag caatttaaat cccataatca atgtgaatat aaaaaatatt
      121 tttagcacaa cgctatgcgc actgtcaccc gctcaacaga tcacggttat cgttcgtttt
      181 ttatactgct cagggataaa aaaaggcccc tgttgaaatt gcaggggcct ggtacgagca
      241 agcatcatat tgggcgacat gatgcaacgg taaaaatcat ttggcctgat ggcgttcaat
      301 gattcctttc atttcagcaa ccgccgcccc gtctacgata taacgcgaat actcgtccgc
      361 gtgttcatct gatgacatgg acaatccttg attacgataa cgcatcggtg aggcttgcca
      421 cgctcccgcg gagctatgga cttccagcac tccggcatcg aggaagtggt gcaggttttc
      481 tgcacggact cctgctccag ccataatgat tggagcatca cgatgggcaa taagttccat
      541 aatttttgat aaaccttgca gtgcgtctga tttttgccct gatgtcagta cccgggcaat
      601 gcccaattcc gcaagattat tgagtgtata taaagggtta gcgcacatat cgaaggcgcg
      661 atgaaaagtc actgccagcg gaccggcagc agccattatt ttttccattc gtggcatatc
      721 gacattcccg tcaacatcga gaacgcccgt caccagtcca ggaaaaccta attcgcggac
      781 cgtgcgcaca tcctcaagaa tggcggcaaa ctcaccgtcg ctgtagcaaa aatcaccacc
      841 gcgtgggcga attatcggat gcacagggat cgtcacccgc tggcgcacgg atttcagtac
      901 ccccaacgac ggcgttaagc ccccctcttt tggggctgcg cataattcaa ctctgtctgc
      961 gccgttttgc tgcgccgtta gtgcacattc catgctgtaa cagcaaattt ccagtaatgc
     1021 catttttgct ccttaattac gctgactgct cgctggcaac gatctcttca atggaccacg
     1081 gatgaaactt aatagttgtt ttgccatcgg tgaccgccag cgtcggattc ggtaatcgct
     1141 catgctcacc ttttggtgaa ctggttttaa ctgagatccc cggtaggctg agtccttcat
     1201 cagaaagcaa tgccagcgca cgggcattca gcgtttctgg atcacccggc agaacgattt
     1261 caatatgttc ccaaccttcg tgtgggtaac gtttttcccc gggccacggt agctccacat
     1321 tggaaaactg ccagtgcgca acctgtaccg gttcatgcaa tttaaacaga caaatcggtc
     1381 tgccattgat cattttttct gacaaaagct cgccacactg ttcaaaaccg cgacgccagc
     1441 gctcagcggt gacgttttga tggcaacgca aagaaatgtg atcggcagtc agcggagtga
     1501 tatccaaccc cagacggcgg gaaagttcat ctaatgcgtg ggtaaatcgc ggtaaatccg
     1561 atgcaatatc ctgcagctcg tcgatagatt gccagttcgc cataatcact cttcgtcttt
     1621 cagtaaaagc gttaatttac cctgttgccc tgtgccaacc aaccgctgat ttcacgccgc
     1681 ttctgatgca atagtgaaaa cggcaatacg ccacgcgcac gttgctgacg aaaacagcca
     1741 tttgcagtat actcccgccc taatttcttt aactggtgcg ggcaattttt gctcgcttca
     1801 tcaatgtaag gtattccggt gaatattcag gctcttctct cagaaaaagt ccgtcaggcc
     1861 atgattgcgg caggcgcgcc tgcggattgc gaaccgcaga ttcgtcagtc agcaaaagtt
     1921 cagttcggcg actatcaggc taacggcatg atggcagttg ctaaaaaact gggtatggca
     1981 ccgcgacaat tagcagagca ggtgctgact catctggatc ttaacggtat cgccagcaaa
     2041 gttgagatcg ccggtccagg ctttatcaac attttccttg atccggcatt cctggctgaa
     2101 catgttcagc aggcgctggc gtccgatcgt ctcggtgttg ctacgccaga aaaacagacc
     2161 attgtggttg actactctgc gccaaacgtg gcgaaagaga tgcacgtcgg tcacctgcgc
     2221 tctaccatta ttggtgacac agcagtgcgt actctggagt tcctcggtca caaagtgatt
     2281 cgcgcaaacc acgtcggcga ctggggcact cagttcggta tgctgattgc atggctggaa
     2341 aagcagcagc aggaaaacgc cggtgaaatg gagctggctg accttgaagg tttctaccgc
     2401 gatgcgaaaa agcattacga cgaagatgaa gagtttgccg agcgtgcacg taactacgtg
     2461 gtaaaactgc aaagcggtga cgaatatttc cgcgagatgt ggcgcaaact ggtcgacatc
     2521 accatgacgc agaaccagat cacttacgat cgtctcaacg tgacgctgac ccgtgatgac
     2581 gtgatgggcg aaagcctcta caacccaatg ctgccaggaa ttgtggcgga tctcaaagcc
     2641 aaaggtctgg cagtagaaag cgaaggggcg accgtcgtat tccttgatgt gtttaaaaac
     2701 aaggaaggcg aaccgatggg cgtgatcatt cagaagaaag atggcggcta tctctacacc
     2761 accactgata tcgcctgtgc gaaatatcgt tatgaaacac tgcatgccga tcgcgtgctg
     2821 tattacatcg actcccgtca gcatcaacac ctgatgcagg catgggcgat cgtccgtaaa
     2881 gcaggctatg taccggaatc cgtaccgctg gaacaccaca tgttcggcat gatgctgggt
     2941 aaagacggca aaccgttcaa aacccgcgcg ggtggtaccg tgaaactggc agacctgctg
     3001 gatgaagccc tggagcgtgc acgtcgtctg gtggcagaaa agaacccgga tatgccagct
     3061 gacgagctgg aaaaactggc taacgcggtt ggtattggtg cggtgaaata tgcggatctc
     3121 tccaaaaacc gcaccacgga ctacatcttc gactgggaca acatgctggc gtttgagggt
     3181 aataccgcgc catacatgca gtatgcttac acgcgtgtat tgtccgtgtt ccgtaaagca
     3241 gaaattaacg aagagcaact ggctgcagct ccggtaatca tccgtgaaga tcgtgaagcg
     3301 caactggcag ctcgcctgct gcagtttgaa gaaaccctca ccgtggttgc ccgtgaaggc
     3361 acgccgcatg taatgtgtgc ttacctgtac gatctggccg gtctgttctc tggcttctac
     3421 gagcactgcc cgatcctcag cgcagaaaac gaagaagtgc gtaacagccg tctgaaactg
     3481 gcacaactga cagcgaagac gctgaagctg ggtctggata cgctgggtat tgagactgta
     3541 gagcgtatgt aatcgatttt tcgtgagagt gaagcctgat cagggttagc cgatcaggct
     3601 tttttattgc catctaaatg tattctgaaa acggacatgc cattgttttc tcactgttga
     3661 gtaagaggcc agaagcgtaa tatccaggcc caggaaaacg ataacggttc aatttaagga
     3721 ataccgaagt gtttaaattt cttgtattaa cattaggcat tatctcttgc caggcttacg
     3781 cagaagatac ggttatagta aacgaccatg acacttcagc catcaaagat tgttggcaaa
     3841 aaaattcaga tgatgatact gacattaacg tgatcaaatc atgcctgcga caagaataca
     3901 atctcgtcga tgcgcaatta aataaagcct atggtgaagc ttatcgttat atagaacaag
     3961 tgccacgcac aggtgcaaaa aaacttgata ctgaacaact taacttgctt aaaaaatcac
     4021 agcgagcctg gctggatttt agggacaaag aatgtgaatt aatcctttca aatgaggatg
     4081 ttcaggattt aagtgacccc tattctgaat cagaatggct atcatgtatg atcatacaga
     4141 ccaatacgcg aactcgccag ttgcagctat accgtaactc tgaagatttt tatccaagcc
     4201 ctttgacaag aggataattc acatcttttt ggcatgtttt gttgcaagct attcctgata
     4261 aatgattgca acaaaacatc cagccttttg actgagttat taaacatact cgcgagcgcg
     4321 taattttttg tctttcagcg ataattcaca atcacttcat tccgttgtac ctttagcggt
     4381 ggaattaacc gc
//
LOCUS       DAZPAB010000253         4367 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZPAB010000253 DAZPAB010000000
VERSION     DAZPAB010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.254"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..398
                     /locus_tag="ACHUPG_003805"
     CDS             <1..398
                     /locus_tag="ACHUPG_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR0863210.1"
                     /translation="EIAPFTVTPEMLEAAREARRQEIEAWRAEQEAKLFTFEWNGRIW
                     NAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADNQQVKLSMPELEELAAAMVQAQVDRN
                     DEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(599..1456)
                     /gene="sitD"
                     /locus_tag="ACHUPG_003806"
     CDS             complement(599..1456)
                     /gene="sitD"
                     /locus_tag="ACHUPG_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR0863211.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1453..2310)
                     /gene="sitC"
                     /locus_tag="ACHUPG_003807"
     CDS             complement(1453..2310)
                     /gene="sitC"
                     /locus_tag="ACHUPG_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR0863212.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2307..3134)
                     /gene="sitB"
                     /locus_tag="ACHUPG_003808"
     CDS             complement(2307..3134)
                     /gene="sitB"
                     /locus_tag="ACHUPG_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR0863213.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3134..4048)
                     /gene="sitA"
                     /locus_tag="ACHUPG_003809"
     CDS             complement(3134..4048)
                     /gene="sitA"
                     /locus_tag="ACHUPG_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR0863214.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         1188 a         1064 c         1082 g         1033 t
ORIGIN      
        1 gtgagatagc cccattcact gtgacaccag aaatgctgga agcggccaga gaggccagac
       61 gtcaggaaat tgaagcatgg cgcgcagaac aggaggcgaa gctgttcacg tttgaatgga
      121 acggtcgtat ctggaatgct ggtcccgact cactgggccg cctgtccccg gtagtcatgc
      181 tggcaaaatc tgtcacagca caaacacata tggcgtggag cgatgccgat aatcagcagg
      241 tgaaactgtc gatgccggaa ctggaagaac tggcggcagc aatggtgcag gcgcaggtcg
      301 atcgcaacga tgagatttat cgccgtcagc gggagctgaa ggaagagctg aatagtctga
      361 aggatttgaa ttcggttagg aattttatcg tggaataaca gaagctgcgg cacgtcgtat
      421 gcaggaacgt gccgcggttg gctggtaaac tttcgatagt gcgagtattg aatgatttct
      481 aggcgttatc gattttacgt attttttgca tgatagaatt cgtacctcct cccaacgacc
      541 ttccatggct ttccggactt ctgtaggctg gcggacgatg ctgtcctcgg atgttgtgct
      601 aattgggtga taacagatcc tgtgtatggg cgtttccctt tgtgcgtgcg ttgatgctgg
      661 taacggtaaa cgtcatgatg aacacgaccg cgaataagac gacaatcgtc ggagctggtg
      721 cgctgtcgat aaaaaaggac agccagacac cagtcattga cactattacc gatatgccag
      781 tcgccagtag gagcgcaata tgaaagcgtt gtgtcaggag tactgcaatg gcgccaggcg
      841 caatgagcag agaaatagag agaatgatac ctactgcttt cagcgtcgcc acaatggtca
      901 gggaaaccat gcacagcagc ccataatgca gccatctcgt atgcaaaccg cttacctgcg
      961 cctgttgata atcaaagctg aacagcaaaa aatcccgcca ttttacgcta ataacaagtg
     1021 taaccagccc ggcaataatc accgtctgga ttatgtcgcc gatggttatc ccgagcatgt
     1081 cgccgaaaag aatatggtca agatgtacgt ctggtttaat tgcaatatac aagatcagac
     1141 ctgcggcaaa catgccagaa aaaacaatcc ccataactgt atcttgcttg attcggctgt
     1201 tgtctttcag gtatcccgtc gccaccgcgc agaatactcc ggcgacgaat gcccctgtag
     1261 ccagaggtaa ccccagaatc caggtaagca cgatgccagg aaaaaccgca tgactcatcg
     1321 cgtcacccat cagtgcccaa cctttcagga ccagaaagac cgacagcagc gcacagggta
     1381 tggaaactac cagtgaaatc agcagtgcgt gactcataaa ggtgaactgc agtggctcca
     1441 gtaataatgc catcatgagc gatcctccag cgctttatgc gcacgccgac gatttgccaa
     1501 aagtccatgg gtgggcgcaa atacaaatgc taaaagaaac agcagtgtct gcgccaccac
     1561 gatgattcca cctgtcgcgc cgtccagaaa gtaactgacc cacgcgccga ggaaactggt
     1621 tacactgcca atagttacag caattatgag caggcgggga aaacgatcgg ttaacaacca
     1681 tgctgttgca ccgggagtca ccaccagaca gatcaccaga aaggcaccaa ccgtttgcag
     1741 cgccgcaacg gtcgaaaccg acagcagggt gaagaagagg atttttaatc tgcccggatg
     1801 taatccaata gcacgggcgt gattctcgtc gaaaaaggtc accataagat ctttccattt
     1861 gaaaaaaagc acgatgatcg agagaatgcc aatgatagtg agttgcagga tatccgccgg
     1921 atcgattgcc agaatattgc ccaggacgat ggtctgaatg ttcacggatg tcgggttcaa
     1981 tgacaccata aacagcccca ggccaaaaaa ggacgagaag atcaggccga tgatggcatc
     2041 ttcttttaag cgggtgcgtt ggttaagaaa cagcatgctg cccgctgcca gtccgccgga
     2101 aaagaaggct cccagagaaa acggaagccc cagcatatat gcccccgcaa cacccgggac
     2161 aatagagtgc gacagcgcat cgccaatcag cgaccagcct tttagcatca gatagcatga
     2221 caggaaagcg cagagaccgc cgaccattgc cgagacccac atcgcattga gcatatattc
     2281 atagctgaag ggttccagta gcacgttcat cttgcttccc tctgcaccgc tgacggtcga
     2341 tgtgccacaa aagggcgttc atcgtcagta ataatgcttt cttccgagcc gttaagtgtg
     2401 acatggcgca gtacgccgct aaaagccagc tccaggttct cggctgtaaa agtggtgtct
     2461 gtcggcccgc ttgccaggac ggtgcctttg accatgaccg tataatcgca aaatgtcgtg
     2521 actgagccga gattgtgcgt tgagacaagc atcgttttgc cttccgcgcg taactccctc
     2581 aacaggctga tgattttcgc ttcggttttc acatcaacgc cggtaaaagg ttcatccagc
     2641 aggatcacat caccctgctg tgcaattgct ctcgccagaa agacgcgttt cttttgtcca
     2701 ccggaaagct cgccgatttg acgatggcga aaatccacca tatcgacacg ttccagcgca
     2761 tcggtgacaa tctgacgatc cctctttttg gctatacgca acatacccat atgcccatag
     2821 cggcccatca tcaccacatc ttccaccagt acaggaaatg accagtcaac ctcttctgac
     2881 tgagggacgt aggcaaccag gtttttctgt agcgcctgtc gcgtgggaat acccagaaca
     2941 gatatctttc cgctggtcag acgcacaaat cccataatcg ctttaaacaa cgtcgatttt
     3001 ccggaaccgt ttaccccaac cagagcggca atagatccac ttggtactgt gaaggatgca
     3061 tcgcgcagtg ctgtgtgccc gttacgccag gtgacagtaa catcattaac gacaatgcct
     3121 gcagattgca tcattatttc tcccttttcc cggctttaat tccctgtacc agcgtactgg
     3181 tagtaacctt aagaaggtcg atatacgtag gtaccgggcc gttttctgtg ctcagggaat
     3241 cgacatagag cacaccgccg tagtgtgcgc cggtttcacg cgcaacctga cgcgctggtt
     3301 tatcggaaat cgtactctcg ctaaagactg ccgggatatg atttttctta actatatcaa
     3361 caaccttacg tacctgctgc ggtgttcctt gttgatcggc attaatcggc cacagataaa
     3421 gctcttttag ccccaaatcg cgtgcgagat aagaaaaagc cccttcactg gtgaccatcc
     3481 atcgctgatt ctcagggagt tccgtaatct gcttacgcag gggggcaagg gtttgggtaa
     3541 tcttggcttt ataagtatcg gcattacgtt ggtaggtttg tgcatttgcc ggatcgtatt
     3601 ttatcaacgc atcacgaata ttatcgacgt aaatcagagc attatctggc gacatccatg
     3661 catggggatt aggtttgccc tcatagggtc cttcggtgat ccctactggc gtcacacccg
     3721 aagagacaat tacttctgga accccattga gatgctggta aaagcgttgg aaccacaatt
     3781 ccagattcat accattggcg agaatcagtt gtgccccctg cgcacgttta atatcgccag
     3841 gggtaggctg atactcatga atttctgcac caggcttggt tatggatgag acttctgcag
     3901 catctccagc cacgtttttg gccatatccg cgatgatggt aaatgttgta atgaccttga
     3961 atttttcagt tgcacttgcc tgaaatgcga tcgagcaggt gagggcgagg caccccaaga
     4021 gcatggttac tttttttatt gagtgcataa tttagtacct atagtgagca aaattagaac
     4081 aaacatagca acggctatgt tttatagcat aacctataca aaatgaaaat aattatcatt
     4141 aacagctttg aggcctaaaa cagggattag gttgtttcct gctggcgaca tgatattgag
     4201 gaaaaaagta gagcagctat taggctttga aatgacttta tgaggaaaat tgttgatggc
     4261 aaaaatgggg caaaaaagag tgggttatcg tagcttattg ttgtcgctga taatgtttaa
     4321 catattgaaa aataaataaa atacttatga gtcagatagt tgtgatt
//
LOCUS       DAZPAB010000254         4218 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZPAB010000254 DAZPAB010000000
VERSION     DAZPAB010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.255"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..1953)
                     /gene="speA"
                     /locus_tag="ACHUPG_003810"
     CDS             complement(<1..1953)
                     /gene="speA"
                     /locus_tag="ACHUPG_003810"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR0863215.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYG"
     gene            complement(1962..2093)
                     /gene="yqgB"
                     /locus_tag="ACHUPG_003811"
     CDS             complement(1962..2093)
                     /gene="yqgB"
                     /locus_tag="ACHUPG_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR0863216.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            complement(2187..2330)
                     /gene="yqgG"
                     /locus_tag="ACHUPG_003812"
     CDS             complement(2187..2330)
                     /gene="yqgG"
                     /locus_tag="ACHUPG_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR0863217.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2441..2692)
                     /gene="yqgD"
                     /locus_tag="ACHUPG_003813"
     CDS             complement(2441..2692)
                     /gene="yqgD"
                     /locus_tag="ACHUPG_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR0863218.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2748..3902
                     /gene="metK"
                     /locus_tag="ACHUPG_003814"
     CDS             2748..3902
                     /gene="metK"
                     /locus_tag="ACHUPG_003814"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR0863219.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         1065 a         1139 c         1016 g          998 t
ORIGIN      
        1 accgtacaaa cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc
       61 ggttttcttc acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata
      121 ttgcagcatg tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc
      181 agggaacacg aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca tattgccaag
      241 gatctcctga tatgcaccga ccataaagaa accgagcatc ggcggattct ctggatcgta
      301 ctccggcatt ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc
      361 gtcagagtca caggtaatat ccagcagcac agcgcgacgt tccggcactt gatccagccc
      421 ttccagcggt agaaccggga acaactggtc gatcccccat gcgtccggca ttgactggaa
      481 cagcgagaaa ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg
      541 acgatgcgca cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcagata
      601 gagttgttct gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg
      661 aatgtcgtgc agatccatct ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc
      721 cggttcgtgc atttcctgcc aggtttccca catgctttgc agcgcacgcg gcgcatcttc
      781 tacgggcgcg gtcggcaccg tatattcgtt acgttccacg ccgatgatat tcgacaccag
      841 cacggtatgg tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg gatgcggcag
      901 accattttct tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt attcattgag
      961 gccatagttt acggagcagt cagactgcga acgggtgcct tcataatcca cgcccagacc
     1021 gccgccgacg tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg
     1081 cgcggattca cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg
     1141 gaagtgcagt agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg
     1201 cagtacctga gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt
     1261 acccgagccc tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg
     1321 ttctgcttca tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt
     1381 gtgccccatc ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccgtt
     1441 gcagacgatg acgctacgag tcatgccagc atgtgccagc actgccatca actcggcttt
     1501 ggaaccggct tccagaccca gcggttcacc cgaatgaatc agggactcga tcacgcggcg
     1561 gtgctggtta actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc
     1621 cctcgcacgt ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa
     1681 acagaacagt gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag
     1741 atcgacgcga gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt
     1801 aacgtcatag tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc
     1861 ttcctgggag ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga
     1921 cgaaggcaaa cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt
     1981 gtaaaacagt taacgactgt cgcagcctgg aactgcgata acaacccata agcacatgga
     2041 ttttccagca gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta
     2101 ttaagcacat aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt
     2161 tttacaagag cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa
     2221 gcgccgagat gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac
     2281 gagtcttcac cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat
     2341 cacctccacg gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc
     2401 tggaagtggc aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt
     2461 tataccccac aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag
     2521 gaaaaatttc cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc
     2581 gggtgaagaa ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac
     2641 tcagactgcc agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc
     2701 cacacaacag tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc
     2761 tttttacgtc cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg
     2821 atgccgtttt agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct
     2881 acgtaaaaac cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca
     2941 tcgaagagat cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct
     3001 ttgacgctaa ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc
     3061 aaggcgttga ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg
     3121 gctacgcaac taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc
     3181 tggtacagcg tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg
     3241 cgaaaagcca ggtgactttc cagtatgacg acggcaaaat cgttggtatt gatgctgtcg
     3301 tgctttccac tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg
     3361 aagagatcat caagccaatt ctgcccgctg aatggctgac ttctgccacc aaattcttca
     3421 tcaacccgac cggtcgtttc gttatcggtg gcccaatggg tgactgcggt ctgactggcc
     3481 gtaaaattat cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg
     3541 gtaaagatcc atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa
     3601 acatcgttgc tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg
     3661 tggctgaacc gacctccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac
     3721 aactgactct gctggtacgt gagttcttcg acctgcgccc atacggactg attcagatgc
     3781 tggatctgct gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac
     3841 atttcccgtg ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt
     3901 aatctttctt cacctgcgtt caaaggccag cctcgcgctg gcctttttct tttacctcac
     3961 cgcggtggat aggcgttcac gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat
     4021 tcttcctgat cacgtttcac ggcaaattac catcacaact gaaaccgatt acaccaacca
     4081 caccagcaaa atatttgtaa tattttcata ttattattca gctttcacag ttgttacatt
     4141 tcttttcagt aaagtcttaa ttgcagatga cagcgtttaa tctatgatga tataactcaa
     4201 ttattttcat gcccttaa
//
LOCUS       DAZPAB010000255         4211 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZPAB010000255 DAZPAB010000000
VERSION     DAZPAB010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.256"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            139..1203
                     /gene="ulaG"
                     /locus_tag="ACHUPG_003815"
     CDS             139..1203
                     /gene="ulaG"
                     /locus_tag="ACHUPG_003815"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR0863220.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1311..2066
                     /gene="ulaR"
                     /locus_tag="ACHUPG_003816"
     CDS             1311..2066
                     /gene="ulaR"
                     /locus_tag="ACHUPG_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR0863221.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2063..2812)
                     /gene="yjfP"
                     /locus_tag="ACHUPG_003817"
     CDS             complement(2063..2812)
                     /gene="yjfP"
                     /locus_tag="ACHUPG_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0863222.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            2994..3323
                     /gene="bsmA"
                     /locus_tag="ACHUPG_003818"
     CDS             2994..3323
                     /gene="bsmA"
                     /locus_tag="ACHUPG_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR0863223.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3472..3747
                     /gene="yjfN"
                     /locus_tag="ACHUPG_003819"
     CDS             3472..3747
                     /gene="yjfN"
                     /locus_tag="ACHUPG_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR0863224.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3864..>4211)
                     /locus_tag="ACHUPG_003820"
     CDS             complement(3864..>4211)
                     /locus_tag="ACHUPG_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR0863225.1"
                     /translation="SGNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRL
                     QQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQ
                     NDLLLRATGGVCV"
BASE COUNT         1120 a         1054 c         1061 g          976 t
ORIGIN      
        1 tccccactca aaatcaacaa caacttccct ggcaaaaagt caaaaaatgg tcattacctt
       61 cctgaaggaa tagtgtcagg attaatcaaa accaatcaca tgttgattcg aattgaacta
      121 tcatgaaggt aaatggcgat gagtaaagtg aaaagtatca cccgagaatc ctggatcctg
      181 agcactttcc cggagtgggg tagctggctg aatgaagaaa ttgaacaaga gcaggtcgcc
      241 cctggcacat ttgcgatgtg gtggctaggc tgcaccggga tctggttgaa atcagaaggt
      301 ggcaccaacg tttgcgttga tttctggtgc ggcactggca aacaaagtca cggtaacccg
      361 ttaatgaaac agggtcacca gatgcagcgc atggctggcg tgaaaaaact gcagccaaac
      421 ctacgcacca ccccgtttgt tcttgatccg tttgcgattc gccagatcga cgcggtactg
      481 gcgactcacg atcacaacga tcatatcgac gttaacgtcg ctgctgccgt gatgcagaac
      541 tgtgctgatg acgtaccatt cattggaccg aaaacctgcg ttgatttgtg gattggctgg
      601 ggcgtaccga aagagcgttg catcgtggtc aaaccgggcg atgttgtaaa agtgaaagac
      661 attgaaattc atgcgcttga cgctttcgac cgtactgcac tgatcaccct gcctgccgat
      721 caaaaagcgg ctggcgtact gccagatggc atggacgatc gcgcagtaaa ctacctgttc
      781 aaaacccctg gcggttccct atatcacagt ggcgattccc actactctaa ctattatgcg
      841 aagcacggta acgaacatca gatcgatgtg gcgttaggat cgtatggtga gaacccgcgc
      901 ggtattaccg acaaaatgac cagcgccgat atgctgcgta tgggtgaagc cctgaatgcg
      961 aaagtggtta tcccgttcca ccacgacatc tggtcaaact tccaggccga tccgcaagag
     1021 atccgcgtgc tgtgggagat caaaaaagat cgtctgaagt atggcttcaa gccgtttatc
     1081 tggcaggttg gcggcaaatt tacctggccg ctggataaag acaacttcga gtaccactat
     1141 ccgcgcggtt tcgatgattg cttcactatt gaaccggatc tgccgttcaa gtcattcctg
     1201 taatctgtcg tttataccgg atagcgtgga gaagttcgcg ctatccggcg tagtatttcc
     1261 tggctatttc aaatatcatc taaaaaaatc aaattttatc ggaatagctc atgactgaag
     1321 cacaaagaca tcaaatcctc ctggaaatgc tcgcacaatt gggctttgtg accgttgaga
     1381 aagtcgttga gcgtctggga atttcgcctg ccactgcgcg acgcgatatc aataaacttg
     1441 gcgaaagcgg caaactgaaa aaggtgcgca atggcgcaga agctattacc caacagcgcc
     1501 cgcgctggac gccgatgaat ctgcatcagg cgcagaatca cgatgaaaaa gtacgtattg
     1561 ctaaagcggc ctcgcagctg gttaatccgg gcgaaagcgt agtcatcaac tgcggctcca
     1621 ccgcgtttct gcttgggcgg gaaatgtgtg gcaagccagt gcaaatcatc actaattatc
     1681 taccgctggc aaattacctg atcgatcaag aacatgacag cgtgatcatt atgggtggac
     1741 agtacaacaa aagtcagtcc atcactttaa gcccgcaggg cagcgaaaac agtctctatg
     1801 ccgggcactg gatgtttacc agcggaaaag ggctgaccgc agaagggttg tataaaaccg
     1861 atatgctgac agcaatggca gagcagaaga tgctgagcgt ggtagggaaa ctggtggtac
     1921 tggttgatag cagtaagatt ggcgaacgcg cgggaatgct ttttagccgt gccgatcaaa
     1981 tcgatatgct gatcaccggc aaaaatgcta acccggaaat cctgcaacaa ctggaagcgc
     2041 aaggggtcag cattctgcgt gtttaaagat gctggcggaa aaatgtcacg gcagcatcta
     2101 acgcctcagg cgtaatgcgg tgacgcacgc ctggctgcca tgaacaggtt agcagtttat
     2161 cccgtcccgt ctcgcttaag gcctgctgca aacgtagtga ttcgtcggca ggcacaacgt
     2221 catcgtcgag gccatgccac agcagtagag gtctgtcccc aagttgttcc aggtggtttg
     2281 tcgcttccca ctctgccagc ggcgcgacaa ttttattgaa ttcattctgc tgtgctgccg
     2341 tttcaggtat cagcggtgga aacagtgaac gggcgagcga tgtaaaatag cccgatccca
     2401 tcatgctggc ggtacatttc accgtggggt ggcgagcggt aattcccagt gccgtcatcg
     2461 cgcccatcga cgcgccaccg actgccagac ggtcatcaag cagccagttt tcttcggcta
     2521 ttgccgcacg taaagtagtg aattcctgca tactttgtag caagatttgc cagaattgat
     2581 ttaaccgccg cgcttcgtcg ccactaaaac ggctaccgtg atcgggcgca tccggcatga
     2641 tcacccgcaa accagcctgc gccagcgcaa cggcaaaata gctatacacc aggctggatg
     2701 aagtaaagcc gtgataaaaa attacgcacg gtaacggggt atctttttgc ccgacaggat
     2761 aagcatgaag aacgggaata tctgccagct cgcgtgattc tatttcaatc atctgtctct
     2821 cctttttgtc tggtatgaca tgaagaaatc cttagcacaa gtttctgctg gcggtattag
     2881 ttcataatgt tgagatgtgg gttacgcttt cgtcacgttt tcattcgaaa atcgcgtacc
     2941 aggtaacaat tcgggaacat tcccccaaag ttaaattaat aaggcactac actatggtta
     3001 gcaggaaacg taatagcgtt atttaccggt ttgccagttt attattggtg ttgatgttaa
     3061 gtgcctgtag cgcactgcaa ggtacgccac agccagcacc gccagtaacg gaccatccgc
     3121 aagagattcg ccgcgaccag acgcaagggt tacagcgaat aggtagcgta agcacgatgg
     3181 ttcggggttc tccggatgac gcattagcag aaatcagagc aaaagctgtc gctgcaaaag
     3241 ctgattatta cgttgtcgtc atggtggacg aaaccatcgt gacaggacag tggtattcac
     3301 aagccatttt gtaccgtaaa taaatcagac aacttacata gtcagcttta cattgctttg
     3361 cgtccctatg cgtttacctg tgcacaataa attaccggct ggaagcttgg gcgtttatta
     3421 ccgcgatgga atgccctgca acatgtgggg aaacgaaaaa tggagctgac gatgaaacaa
     3481 ttacttgcct caccctcgct gcaattagtg acttatcctg cgagcgccac ggcacagtct
     3541 gcagaattcg ccagtgctga ctgcgtgacg ggcctgaatg aaataggtca gatctcagtt
     3601 agcaatattt caggggatcc gcaggacgtg gaacgtatcg tggcgttaaa ggccgatgaa
     3661 caaggtgcgt catggtaccg cattattacc atgtacgaag accagcagcc tgataactgg
     3721 cgcgtacagg cgatactgta tgcatgagtg tcatctacag caaatgaaca atgccggatg
     3781 cggcgcgagc gccttgtccg gcctacaatt ttcacatatt tcatttagtt aatcgaaacc
     3841 agcgtcgcat cagtcgtata cgcttacaca cacactcccc ccgtcgcccg cagcaataaa
     3901 tcattctgga tctgctctga caaccgtacg ccgccgcgcg tatctaacat cacctgacac
     3961 cacgcctgcg ccattggcgg agaagcatat ttcaacattt gcgcaccgca gcccagcagg
     4021 aatagctgat gagtaatctc tcgccccagt tcttcagctg gcttacgcag ctgctgctgt
     4081 aaacgacgaa ccgcgcgatc aaaatagcga tcctgccctt tcacttccac aaatgcttcc
     4141 gacaataagt cgtatacgcc cgcttgctta ttgagaacgc gcaacacatc caggcacata
     4201 atattgccgg a
//
LOCUS       DAZPAB010000256         4209 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZPAB010000256 DAZPAB010000000
VERSION     DAZPAB010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.257"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..209)
                     /locus_tag="ACHUPG_003821"
     CDS             complement(<1..209)
                     /locus_tag="ACHUPG_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR0863226.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHUPG_003822"
     tRNA            complement(324..399)
                     /locus_tag="ACHUPG_003822"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHUPG_003823"
     tRNA            complement(406..480)
                     /locus_tag="ACHUPG_003823"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHUPG_003824"
     tRNA            complement(597..681)
                     /locus_tag="ACHUPG_003824"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHUPG_003825"
     tRNA            complement(690..765)
                     /locus_tag="ACHUPG_003825"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHUPG_003826"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHUPG_003826"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR0863227.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHUPG_003827"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHUPG_003827"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR0863228.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHUPG_003828"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHUPG_003828"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR0863229.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1046 c          937 g         1137 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAZPAB010000257         4198 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZPAB010000257 DAZPAB010000000
VERSION     DAZPAB010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.258"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            84..752
                     /gene="ykgH"
                     /locus_tag="ACHUPG_003829"
     CDS             84..752
                     /gene="ykgH"
                     /locus_tag="ACHUPG_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFR0863230.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(995..1690)
                     /locus_tag="ACHUPG_003830"
     CDS             complement(995..1690)
                     /locus_tag="ACHUPG_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR0863231.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1683..3110)
                     /locus_tag="ACHUPG_003831"
     CDS             complement(1683..3110)
                     /locus_tag="ACHUPG_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR0863232.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3121..3840)
                     /locus_tag="ACHUPG_003832"
     CDS             complement(3121..3840)
                     /locus_tag="ACHUPG_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR0863233.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1119 a         1085 c          869 g         1125 t
ORIGIN      
        1 ttgctgatat ttaacccgaa cattcctgat acagactatt aatacaacat tatctgcgac
       61 agattttatt ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg
      121 agattttatt tcatctgaag aaaaagattc gtgttatcct ttttattatg gctatctgta
      181 tggcaatggt gctgttgttt ctgtatatca ataaagacaa tacaaaagtg atttacagcc
      241 ttaaaataaa ccagacaacg ccaggtatac ttgttagctg tgatagcaat aataattttg
      301 cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt tttcagacca
      361 gcccagatat caagaacaga gaaataaggc tggaatggtc aggagataag agagatttac
      421 caactgctga agaggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag
      481 aataccataa tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg
      541 agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg
      601 gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg
      661 ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg
      721 tagatgaata ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg
      781 tataaagctg aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc
      841 tttttcactg taaatatcct ggcattacta atgcctggat atttacagaa gagcagcata
      901 accatgacgg aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc
      961 ttcttcaggt ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca
     1021 ccgctttcac cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgagc
     1081 tgggaccact gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat
     1141 gcaatttttc tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa
     1201 gagaagattc cgggagcagg ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca
     1261 cgcctcccga ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg
     1321 cctgcgagat attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct
     1381 gcaaacgttc actaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact
     1441 gatctcccag ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca
     1501 gctcacagcg cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct
     1561 gttggttaag ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt
     1621 gcggttcaag tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc
     1681 gattatccat tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct
     1741 ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt
     1801 ggtgttttgc cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact
     1861 ttccacaatc ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct
     1921 tttgcggtga tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc
     1981 ggaatacgca ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa
     2041 tctttaaaat ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga
     2101 taaatagagc catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa
     2161 gccccgcagc gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc
     2221 ccgttatcga caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc
     2281 ggccctgtca gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc
     2341 atggtgatca atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact
     2401 gcaatatgcg ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg
     2461 cataccgaac cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg
     2521 aggaaatctt cacggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc
     2581 ccctcatagc ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt
     2641 gccgggacca caacatgaga tggcggatct tgatccagct ggagaatata ttcacccaga
     2701 tcggtttcaa tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg
     2761 gtcaccatcg atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa
     2821 atgtagcggg tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc
     2881 actttttctg agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc
     2941 tgggcggccc gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga
     3001 tttgccccaa tacgctgctg cgcgcttgcc accgctttgc gcatgatcgg atcttcaatt
     3061 tgctgacgga tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc
     3121 tcagcggctc atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg
     3181 taatcgccca ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg
     3241 gacttccatc aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt
     3301 gaccgagaac gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc
     3361 cagtccacgc acatttttca gcagcgtaag tggctcgtcc ttcactccca gcttacgggc
     3421 caggctacaa gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac
     3481 ccctaattta ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc
     3541 acgtgatgcc cattcaggtt catccgccag atacgtcggg taacttttta tggcataggt
     3601 gcaagagcca gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt
     3661 tttcatccct ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca
     3721 tccctgtttc tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc
     3781 tcgtgccatt cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac
     3841 aactaatact ccattacttc atgcccattt atgcccgatg tttccccccc accgggggat
     3901 caatcatcaa taaatatggc gttctttttt aattggctag caacatacca cccctcattt
     3961 gtatgacaaa tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt
     4021 cggcatcgct cacattttat ggggaaatcg acgatcattt ttggcataag aaatccattt
     4081 aaaaatggct acatccctta tacctattca gagctactta ttcaggggtt attgagatat
     4141 acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagtaa tagtcgga
//
LOCUS       DAZPAB010000258         4182 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZPAB010000258 DAZPAB010000000
VERSION     DAZPAB010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.259"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..186)
                     /locus_tag="ACHUPG_003833"
     CDS             complement(<1..186)
                     /locus_tag="ACHUPG_003833"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR0863234.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
     gene            complement(327..2360)
                     /gene="metG"
                     /locus_tag="ACHUPG_003834"
     CDS             complement(327..2360)
                     /gene="metG"
                     /locus_tag="ACHUPG_003834"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR0863235.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2492..3601
                     /gene="apbC"
                     /locus_tag="ACHUPG_003835"
     CDS             2492..3601
                     /gene="apbC"
                     /locus_tag="ACHUPG_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR0863236.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3863..4144
                     /locus_tag="ACHUPG_003836"
     CDS             3863..4144
                     /locus_tag="ACHUPG_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR0863237.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1016 a         1043 c         1062 g         1061 t
ORIGIN      
        1 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
       61 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      121 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      181 tctcattctg catccctata attttatcca gttgaatgca gatgctacca gtatttatac
      241 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      301 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      361 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      421 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      481 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      541 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      601 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      661 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      721 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      781 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      841 aggaaggctt ctgcacgctc ggtcagtttc ggcagtacag gcttcaggta agtcatcagt
      901 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      961 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1021 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1081 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1141 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1201 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1261 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1321 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1381 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1441 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1501 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1561 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1621 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1681 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1741 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1801 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1861 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1921 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1981 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2041 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2101 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2161 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2221 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2281 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2341 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2401 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2461 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2521 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2581 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2641 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2701 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2761 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2821 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2881 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2941 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3001 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3061 caatgcgatg gtgtggcgtg gaccgatggc gggcaaggcg ttaatgcaga tgttgcagga
     3121 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3181 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3241 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3301 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3361 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3421 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3481 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3541 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3601 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3661 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3721 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3781 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3841 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3901 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3961 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4021 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4081 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4141 ttaatttggc aacctgattt taattgcatt tagaaagacg aa
//
LOCUS       DAZPAB010000259         4175 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZPAB010000259 DAZPAB010000000
VERSION     DAZPAB010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.260"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..338)
                     /locus_tag="ACHUPG_003837"
     CDS             complement(<1..338)
                     /locus_tag="ACHUPG_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFR0863238.1"
                     /translation="MYTESNIKLSCNALWQKCVLATLYRAAATHPKIKLLQFKLIPCP
                     GMNKAIPSLIADFTGELHRLISEHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLM
                     VLLLNNDVWC"
     gene            complement(366..928)
                     /locus_tag="ACHUPG_003838"
                     /pseudo
     CDS             complement(366..928)
                     /locus_tag="ACHUPG_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(992..1338)
                     /locus_tag="ACHUPG_003839"
                     /pseudo
     CDS             complement(992..1338)
                     /locus_tag="ACHUPG_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            complement(1445..2008)
                     /locus_tag="ACHUPG_003840"
     CDS             complement(1445..2008)
                     /locus_tag="ACHUPG_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFR0863239.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3383..>4175)
                     /locus_tag="ACHUPG_003841"
     CDS             complement(3383..>4175)
                     /locus_tag="ACHUPG_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863240.1"
                     /translation="GQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
BASE COUNT         1329 a          920 c          748 g         1178 t
ORIGIN      
        1 ttacaccaga catcgttatt aagtagcagc accataagat aaggagtggc atcgttagcc
       61 acagcttccc tactccagag gtaatataaa ggggtgggct cgactgattt atctttacgt
      121 cgcttacact gcaaatgttc agaaattagt ctatgcagct caccagtaaa atcagcaatc
      181 agagaaggaa ttgccttatt cataccaggg caaggtatta atttaaactg taataattta
      241 attttaggat gtgtagcagc agcccgatac agagttgcaa ggacacactt ttgccagagg
      301 gcgttacagg aaagcttaat gtttgattct gtatacataa taaatcacct tatagttaca
      361 ataggtcaaa aaccgctgta gccaaagtta cgctggcctg atgctttagt gtcgagcttc
      421 gtcaaataat ccagacgtac caataagcgc tggtactgct ctgggaaatt aggatcgtta
      481 atatccagga tgtaacgtcc attagcaggg aaatgaacta atccctggtg atcttccagt
      541 tccaacccta aggcactgta ccaggctccg gtaattaacc ctctcagggt gtcagagcgc
      601 tcatagtttc ccagatggta ataggcgtct ttgttaaaaa aaggcagacg tgataatgac
      661 atttaaagga ctgcgtatat tctttcgccc agataataca aaccgacgtt gcgtaggtgc
      721 gtttgccttg ttgtttttta cggtgttgat ctgcattaat tttcgcattc agcgagttac
      781 ggaaacgaga tatctctccg gggatcaggt tagggaaaca ggtaatgata tccccgttgt
      841 caatcacagg agggtaatgg agatcccccc gtattgctgt tattctggaa gtgtttttaa
      901 tattcgtatt gatagttctc agagatatat cacactctag atattttaat taagtatcag
      961 ttcgatatcg gcatattgta cactacctca ggtagatgat gagatcagat acagggatca
     1021 actgacggtg agcgcagtcc ccacatttta tccagggctt actgcatatt cccaacagcc
     1081 attcgttagc caggtagacg aataccctga tttgccgctg gttttgcttt cctatcgaag
     1141 ggcccataca tcatcacgcc cacgaaacaa ccgacgaaat aacgcaactt tctcatccgt
     1201 ggataatacg aaaacgcact gcacagggct ctgcggttta cgtcgccatt caatcccatg
     1261 agcttcaagt aatgagacca gccgggtatt ttctgcccgc agtactgcta gttcatcgct
     1321 gtcagattta taaaccatat gcgttatcac ttgtacctgt ctcccggcgg caccacgact
     1381 gaaattcgtg tgaaggcaaa tccacgcgat aacttacgat agttcggaca taagccccca
     1441 taattcagtt atgtcgtggt cgaccccggt tacttttttc gggaagcctg ctacggccca
     1501 gaataatacc gtgttctttc tgatcctgtt tagccagata gctaagggcg taacgtaagt
     1561 cgtctaccgc agatttatca ctatagtgaa tcacgtgatc gatacgcacc gggtatttgt
     1621 ctttagcccg gcacagatga aaataaccct ccccttccgt gatccgcctc cagatatctc
     1681 ccagttgcct ggatatctga taagagttct tgtgacgttg tccatccagg tagccgataa
     1741 aatgaatatg aagccctttg tctggtgtat attccattac ccagtaatat cccgccaaca
     1801 tcgtctgggt ttcgctaagt aaacggtata tttccataca catactgtgc ttacaggaat
     1861 gcccgaaact gggcgtgtct ttcctgtaag caaaatcaat tctgaaaggt aacagtttag
     1921 aaaaacgttg aaacatacca tccatgtgtt cattcacgtc tttcagaatc atgaaatcca
     1981 ttttgtaatt gggattggca ttatacattt tatagtcctt actttataaa actcacagga
     2041 agagagaatt acgtaaatac tgagagtaga gaaacgccgt accacggcat taataaacga
     2101 agtaaaccag cagcacagac aacctgataa aactgttttg tattaccgca ttactgtgat
     2161 gaggtaatac ttcagaatac agtgagactc tcttaaactg agaaccagta cgaattacaa
     2221 tggaaaaaca cacctcatag aattgagttt caggaagcac cactaaattc tttacaggaa
     2281 gtaatctgac attcacaaaa ctcagtatcg gctagctaca ggcaacatcg ctattaatat
     2341 tgtcgaacca ttaagtaaag ccgagagaga ccacgggtat ttaatcatta gcataaaaga
     2401 ataatggtat acagaacata cccacacacc ataagaagat tgttccatac agaaaagatc
     2461 atatttacct atacctataa actgtaacgg agtcataaca cgctctaata gtgttccgat
     2521 acgtaaccag tgttaagtac gtacaggaca acattacaca cataattcac catacagctt
     2581 cccccatatg cttcactgcg tagtgtatgg agatatatgc attacacaca tacagaatac
     2641 ttagcataaa gaggagtgac attaccacgt aaagagataa gttatacaac aaacatacaa
     2701 acaatacggc acagacacac aataccataa ctccaactaa taatactaat taaccttaat
     2761 tatttatggt atatgaaata aatgtgattc tcccggaact aactaaccta accagacaga
     2821 tgatcactgt ataaaaactt cagttaatac tcatttgttt tgtcgactca gtatataatg
     2881 agtgaccagt aatcaggata atcacactta cataacctgt aaaaaagaac aagaagaata
     2941 agccactaac ataatgcaaa atggcatctg aatcgatcgc agtttggcat tcgaatcgat
     3001 catgatttgg cccaatccgt gatcgaatag agcggaatac gcaacataat tcaccagaca
     3061 agagcttaca ccgaattaca gattatgtat taatgtcaaa actggcatat tttacagata
     3121 caatgaacat gcaacccatc aatctaatag gggcggtatg tacatctgcc catgaatggg
     3181 atggcgctaa gaatacaaaa caattagaca tagatcacaa aaacacacca ttctaattga
     3241 aaaacaaaac aaacaaataa atcatactat tatagaattt tctcttgcca gacatagata
     3301 atacccctgc taattttgct aatggttagc aaaattagca gggtatataa agattattga
     3361 cccaaaacac actcagatga attcacgaca gctcaggagg gttattcctg atgtcatctt
     3421 cacaaataat ggttaacgca tcatatatca ttctgcgtct gttcctgttt tcggccttat
     3481 cgccatacaa catcatcaca tttttaattt catcgaccgg tgatttcaca tagtgaacta
     3541 atacaccaag cttctcaaca cattgcagta aagtatattt cttactgcct gtgaaaagcc
     3601 ctatcatagt ttctttacgt tcgcgactgg ataatacttt gttttccata atgacttctg
     3661 ccagcgtctt accatcataa acatactctt tatctatcag aatatttatc ttatccagca
     3721 ccttctctac gtcatcaagc aaacctgtaa tatatggggg cgcaatatat ggtttcgtac
     3781 tgatcttttc aagcatacaa ttatacatct taataacacg catcagggta aaattgttcg
     3841 catcatactg tatttcaccc tccttatata caggatcaat aagtggtatt ttttcaataa
     3901 atgcatcctg ataggctttt acatggtcaa gccaataaaa caacgagcca tactttatat
     3961 tttttctatt tactttaacc tccagtgctg tccgaagcaa cgataatgcc cccctgacat
     4021 atcctgaagg taattcacca aacaatcctt ttctttccat gtcatcataa tgctgcagca
     4081 catcattaat tttatttttc cttatcagac aaatgtacac cgcaagtgtt tcgtagcagc
     4141 tggtcacatg aacagcctca ttctgcaact gccca
//
LOCUS       DAZPAB010000260         4159 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZPAB010000260 DAZPAB010000000
VERSION     DAZPAB010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.261"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..323)
                     /locus_tag="ACHUPG_003842"
     CDS             complement(<1..323)
                     /locus_tag="ACHUPG_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR0863241.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTL"
     gene            complement(333..881)
                     /locus_tag="ACHUPG_003843"
     CDS             complement(333..881)
                     /locus_tag="ACHUPG_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR0863242.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(881..1588)
                     /locus_tag="ACHUPG_003844"
     CDS             complement(881..1588)
                     /locus_tag="ACHUPG_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR0863243.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1603..2280)
                     /gene="ydjY"
                     /locus_tag="ACHUPG_003845"
     CDS             complement(1603..2280)
                     /gene="ydjY"
                     /locus_tag="ACHUPG_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR0863244.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(2285..2995)
                     /locus_tag="ACHUPG_003846"
     CDS             complement(2285..2995)
                     /locus_tag="ACHUPG_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR0863245.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(3162..3968)
                     /gene="xthA"
                     /locus_tag="ACHUPG_003847"
     CDS             complement(3162..3968)
                     /gene="xthA"
                     /locus_tag="ACHUPG_003847"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR0863246.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
BASE COUNT         1060 a         1065 c         1059 g          975 t
ORIGIN      
        1 tttaaggtgc ggaagttttc gccgttcacc cagagcagat ccaccgagcc gcccgtttta
       61 cgaccggcag cagcttcggt ctgaatgcgc ttcaccgcgt ctgcggcatc cgcgagacga
      121 acaatcttca ggtttatagc gtaatgggtt ttcatctcgc cgctaaccca gtcgagatag
      181 cggttaattg cggtatcgcc gccccaggcg ttaaaccaga cggtttgccc tttggcctca
      241 tttttaattt cctgccagtc actggcatgt gttgccaaag aaaacagcga taacaatccc
      301 agcaaccacc cacaatggcg catcttccct ccttaagaac gtttttccgg aatacggcac
      361 agcccctgag cgggaatgtc catggcagaa ttaaaacgag ccgacaggtt ttgcaggccg
      421 atcaatgccg tcagttcggt gagcgcctga gcgtcaaaat gcgcagccag tcgggtacgc
      481 agggcatcat cgactgttgg cggtgtgacg cttgcggctt cggcgtactc cagcgccagc
      541 cgttcttcat cgctaaagag cgggctttgc cgccagtcag ccactgccag cagtttatcg
      601 gtgctgccgg tacgctcggc gactttcata ctggtgatat ccacgcaaaa ctcacacagg
      661 cacatttgcg caatgcgcgc gctgacaagc gatcgcacta ccggatcgag cggtgagcgt
      721 ttgcgctcca gccacccaac aaacattgac accaaataaa agataaacgg gatccgcccc
      781 caccagcgaa tcgggcttag cacctcgccg taatggcgac gatgcagcca cgcctgcgga
      841 cgtaaaatga aaggaatttt gctaagcggc ggtaaaccca tcagtcattc ctcttctggc
      901 gctcaagcca tattttcttc gccataaaaa tcaccacggt cagggcaaac agaataaaca
      961 gttcggtgac aaaccagaaa gtaccgcctg ttaacatgct gcctgcccag gaataaacaa
     1021 tagtcgccgg taattgacca agcccggtgg cgataaaaaa cgagcgaaaa cgtattgaag
     1081 tcagaccggc agcatagctg attggatcga aagggacaaa aggcaataac cgacagacca
     1141 ggatggtgtg tttgccgtag cgagtgaaaa agccgtccat actgtcaagc acggttttgc
     1201 cggttaattt ttccaccact tcgcggccca tcactctggc gataaaaaag cacagcgccg
     1261 cgccggccat cgaactggtc cacgacagca agccgcccca gaacgcgcca aacagcgacg
     1321 cattggcaaa ggtgatcaaa aacgcaggta gcggtgcagc aatggcctgt aaaatcatca
     1381 acaagaacga gacaactgcc gccagtgcgc cgtaagactg aataaagcgt tctataccct
     1441 gttggtccac ggcggcaaac gccgcaacgc tgcgattgat aaactcatgg acgccgggaa
     1501 gcagcgccca tgcgagcagc atagcgaaca acaggatgat gagggttata cggtagtacc
     1561 agattttacg cgattgtatc ttcatcatcc tttatcttgg ctttattcgg tgattttaaa
     1621 ggtaaccgtt gccagcgtgt tatccgccgg gagaactgag gcattgcctt tgaatttcac
     1681 ttcgccgtgt ttttccaccg caccataagt gtatgttgca ttgctgacga tgccgaccgg
     1741 gcagctatcc agacacacca ggcaacccgt tttcttctct tctgctgccg ttaaattacc
     1801 gccaaagcgc atgtccagtt tcttgccatt actgtcaaca atcacttcat cgaaggaata
     1861 cgcttttgcc gccccttgcc agttgaccga aatatccagt ttgctgcctg tgacatgagt
     1921 cgtttcttta ttatccatcg tcatgttttc gcccggcgta ccacctgcct ctttcagggc
     1981 ttcataaaat gctttcgggg gcgcataagc cataaacagc gatttatgtc cgttggagcc
     2041 atctttaaac acaataccgt gacgagtgtc gtcggtgagg aaacgaccat taatttgcac
     2101 cagcatggtt accgtatggt tattggcatc gacgcgcagc ggttggctgt tcgaaagtcc
     2161 gtcgtattcc acttttgccg ccgcattctc tttttgatcg cagccgctaa gcccggcaac
     2221 agccagtaag cagagtgcag ccagtccttt tttccatgac actgaataat gttgcgacat
     2281 cgttctatcc ttcatttttt gggtgagtaa gtgggctgcg gcgcgaagca gacagatcca
     2341 catgtttgtg tcgggcgtag agttttgcga gctggatgag aataaacagc gccaggcccg
     2401 ccagacagag ttgtaaaata aagcgcagcg taatgccttc attggcgaga tcgcttgcca
     2461 tcacggtata aataacaata ccgggcaggg tcgtgagtgc cgaaataagg gtataaggcc
     2521 agaaggcgat ggtggtgaat ccgtaagcgt aattttgaat attgtaagga aacaacggga
     2581 ttaagcgggt cagaataaga aaatcaatac cgttacgcgc aatgcctttt tcaatggcct
     2641 ggaaggtatg gctatggccc acgtatttca gcagtaaatc acgtcccagc cagcgcgcca
     2701 gcaggaatga gcacgaggag gccagcgttg cggcaattaa tgagagttgt gtccctaaga
     2761 gcgggccaaa aactattcct ccagcgatca ccagtatgct tcctggtaac agaaagaggg
     2821 gggcattgat gaataacaga atatagaggc tataaccgaa caatccgctc tggcgaatga
     2881 gtgtctgtaa gtggggtaaa tcggtgagca gatcgaataa accgaaagcg tggatagcgt
     2941 aaatgaccag cgcaaaaata agacaggcaa acagaaagtt acgctcagcg ttcatcgtgg
     3001 agtttcctat atcaaacttg ataatagaga agtggtcaga caactaaaaa cgcagtgacg
     3061 gtataacaaa ggacggcagg caacaaatca ttactgacag tttgttgatt tttctcaagg
     3121 ttaattctcc tgacccagtt tgagccagga gagctgctaa attagcggcg gaaggtcgcc
     3181 cagacggggg cgtgatcgga cggtttttcc atgctgcgaa tttcatagtc gatgccggtt
     3241 tctacgcaac attctgccag cggttggctg gcgagcagca ggtcgatgcg cagaccacgg
     3301 ttatcgtcaa aaccttttga gcggtaatca aaccatgaga aacgatctgc tgtttgcgga
     3361 ttagcatggc ggaaggtatc gaccaccccc cagctcatca gcctgtccat ccattcgcgc
     3421 tcttccggca ggaaagagca tttaccggta cgcagccagc gcttacggtt ttcttcgcca
     3481 atgccgatat ccagatctgt agggctgata ttcatatcgc ccataatcag taccggatta
     3541 tcacgtttga gttcggtttc caggtagttt tgcagattct gataaaactg cgcttttgcc
     3601 gggaatttta tcggatggtc gcggctttca ccctgcggga agtaaccgtt gatcacggtg
     3661 acattaccca gcagtgaggg gatttccgcc ataataatcc gccgctgcgc ctcttcgtcg
     3721 tcaccgggaa agccgcgacg cacggcaatc ggcgtctctt tggtcagcag cgccacgcca
     3781 taatggcctt tctgcccgtg ataaaacacg ttgtagccga gcttcgccac ctcttcgagc
     3841 ggaaacatat cgtcatgaac ttttgtctcc tgcaggccaa tcacatccgg ttggtgcttt
     3901 tcgacgatgg cttcaagctg gtgaggtctg gcgcgcaggc cgttgatatt aaaagagaca
     3961 aatttcatag tcgctgccat ttattcagat aaagagtgcg tggatggtag cagaatttcg
     4021 cgttgcttac cgcctgttgt taagtgattt gatcgatggt tgttaatgat gtcaacgatg
     4081 gcgcaaaaaa tgcccgcttt tggtgcgcgc tgcgtcagaa tggcgcagta atttccagta
     4141 aatttcgtca atgatcaca
//
LOCUS       DAZPAB010000261         4145 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZPAB010000261 DAZPAB010000000
VERSION     DAZPAB010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.262"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..433)
                     /locus_tag="ACHUPG_003848"
     CDS             complement(<1..433)
                     /locus_tag="ACHUPG_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863247.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYI"
     gene            complement(426..1190)
                     /locus_tag="ACHUPG_003849"
     CDS             complement(426..1190)
                     /locus_tag="ACHUPG_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0863248.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGIAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            1436..2197
                     /locus_tag="ACHUPG_003850"
                     /pseudo
     CDS             1436..2197
                     /locus_tag="ACHUPG_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(2304..2816)
                     /locus_tag="ACHUPG_003851"
     CDS             complement(2304..2816)
                     /locus_tag="ACHUPG_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863249.1"
                     /translation="MKKTLLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNT
                     IQPVKGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNN
                     PDHKLKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYT
                     IRTQAYIYAE"
     gene            complement(2955..4103)
                     /locus_tag="ACHUPG_003852"
     CDS             complement(2955..4103)
                     /locus_tag="ACHUPG_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0863250.1"
                     /translation="MSITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQP
                     LGNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDR
                     KLSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAH
                     QSQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDG
                     TLALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTS
                     QLVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDG
                     IAYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT         1276 a          905 c          717 g         1247 t
ORIGIN      
        1 aaatatagac attattaaac ttgcttagcc tcgcattgcc tttacctgtt aaagatgcat
       61 cgagaaaatt gagtctgaag ttctcatcct ttatctgatt ttccagtccc gaaactttgg
      121 ccttagcatt acctgagatc cgtccgttac tatctatttt gtaggtaaaa aaatacgaat
      181 gggaaaattt cctcggtgta tcttcatcag ttaagccaac aatcgtaaaa ctaccgctac
      241 catctttttt taaaatgact gaaaggttag cagagtagta taaagaatga tctgttttag
      301 catttcttgt taacgtcgac gtacattcaa gagacgttgt gctaccatca cgagccttcc
      361 agagagcaaa aaaacacacc tgtagcaatg tgaaaacaag tattaatata aaatacattt
      421 tcttattagt catataatag cacctctgaa caaattgcat aagtttcgtc attagaggat
      481 tttatacatc ttgataaata ccgctgccct tccccaagca aataatgcat actatttaca
      541 tcatatataa aatttgcacc tggcacacag ggtaaatgct ttttcgaaat ttcagcggcc
      601 tctatttgag atttagcgat gccaatctcg ttaagattct ctgaaagata taaatcacag
      661 gtgtcaatct tacctgattt aaccaatgga taatccgtta tactgtaggt atctttaaaa
      721 gccacggcaa acatttcaat aataacaatt actaccacaa gcgaaatcaa aatgcgaaaa
      781 agaggaatac tccttttgcg ctctactttt tcaacaacat cttcttgtaa atgagaatca
      841 tcataagatt gtttacttat tgttatgttt tcactgatgt aaaagcctac tttgggtatc
      901 gtaagaatca cattttcttc aacaccaata tttttaaaac ttttgcgtaa cagggatata
      961 tattggttca atgtgttgtt tgacagatca aatgaaaatc tctccaaaac gctatcaatg
     1021 attgagttcc tgtcaaccgc tccaccatta ctttccagta acactgccaa aatacacccc
     1081 gccgtagctg gaaaatttat ctcatcctgg gtatttacat tcgagagcga gcatttgttc
     1141 ggatcaaaaa taatcgcgtc attgataatg aaaaaacatc tccgctccat gcaggcaaca
     1201 acacaaaata agattttgtc attttagcct attcctggtg gaactgacac aagaaactaa
     1261 aatacaaata taaccctgaa aaagtcatat aaaacttata tccacagaat aaaaaatcaa
     1321 acatgtcagt tttatcaata taaagtatcg caaggcgaaa agtgatgcaa gaatactcct
     1381 aagtaaatga aagcgcacca catgaaatta tcaacacatg taatttcatc attaggtgct
     1441 atcgaaaata actcaggagc tacttaagat gaataatgtc ttactcatga gacccgatga
     1501 ctcagaattt atgatacctg atgactggtc agggctaaac gcaaaaggca ttacatacga
     1561 acaagataat agagtgacag agtcttttct gccttttaaa tctttttatt gttcaccggt
     1621 gtgcacgaca gttgaactca tctcataaaa taattgtttt gctatgggta tccgctacct
     1681 tctggaaaag aaaagagact taaggctaaa tcactttaaa gccttctctc ataaatgcga
     1741 gacaaaacct gacgtgttaa ttatgaccgg gtgcaccgtc gctgatactt atagtatatt
     1801 ctcacgcatt gttgaagaaa atataatgaa aacgatgaca cctatcgtca tcattactgg
     1861 gcatcactca gaagatcttt gttctctggc atcgaaactg ggaggagtta aaataatatc
     1921 accgacagaa aagattgcaa aaatagaaaa cgctatcaat gatgctttta acagcaagcc
     1981 taaaaaaata agatttttaa cagatatact ctctcctcaa caaattgaga ccatgaaaat
     2041 ggttgcgcag ggtagatccg ttaatgagat ttcagccgca atgaatatta gcatcaagac
     2101 cgtgtatgca cataaagttc gtgctatcaa taagctggga attaagaaaa aagtttatga
     2161 agcgcttttt tataaagcgc taattcagtt caactgatgt aaaaatcacc ctcgcttaat
     2221 aaataatata ttctatgtct ttttttataa gttaaaaaag gcgggatatc aaatcccgcc
     2281 atgaaattaa ctaatcatct gcattattct gcgtagatat aagcctgggt acggatggtg
     2341 taagagtctg gctcaacaac ctgttctttc atatgggaaa cgacgcgata ggcaccacct
     2401 gttccagtat ttttcagcat catataacgc ttaccgccaa tctcttcgga ggcagctgaa
     2461 ggaggatcct gctcaagcgt tgtcagggaa actttcagtt tatggtccgg gttattgtca
     2521 ccagaaatta ctgcatccgc tgaagtactg ctctccgcat aaatatcatc cgggaaagtt
     2581 acagcatagt cgtggctctg cccatcagat gcgccaacaa tacctgttgc caaaggcgtc
     2641 catgcagtta acgtaccgct atatacccct ttcactggtt gaatggtatt attaacaacg
     2701 ataggcttgg tcaaagtaag attagctgac gcttcagtac cctggcttac taaagttccc
     2761 gcctgagaag caaaagctgt ccccgccagc atgattgcta acaatgtttt cttcattata
     2821 ttttctccat gattttcatt tacactgcaa aatgcagttt ctttctttgc atttacgtgc
     2881 tatcgcgaaa atgcttccac caaataatgg aaacatcttc acaaaaataa attatgagtt
     2941 tcttaattta gaaactataa acatgtcgcc gctatctggt ataaccctga tgcacttttc
     3001 ttcgcaggaa gttggtattt tgcgatacag gattgatctt tagaataacc ccatttcaca
     3061 ttaaactcgc cttccgcact caagccagaa acataagcaa taccatcttc acctacaatg
     3121 ccggccatat tttcagtgtc tttgctataa ctaatcacag cgccgaaggg gatgggttta
     3181 ccctctttct gttttaaggt tactaatcct ttatccccgc tcttcactgc caatgtcgca
     3241 ggcacaacgg ctccagctgt cggtacaacc aattgactag tttgtggtaa gtcgtaattc
     3301 ttacctatcg ttttttcatc cagaataaca tcgtctcttc ggtatggctg cactggcagt
     3361 attgcatacc cacgaaaatc agtactcagt cccggccagc cattaacgtt aacccccgtt
     3421 gctccgggtg cctctataag ggctaatgtc ccatccacat tttgaccaaa tgtaagtcca
     3481 tcccttgtaa taagtatccc accgcttaaa tttgcaccta gctgtttcat gttttcgctg
     3541 gagctgtaat ggctactaaa ctgaccatat gatccatttt tgccaacttg cacaatgttc
     3601 atcgtgctct tcggttgtga ctgatgagcg ttagttatat ttgtcgctac atcccaccat
     3661 acaccaccac tattctgacc tgataatgaa acctgatagt tattaccctg ctctcccgaa
     3721 cgactcatct gtgaagacgc ataaatttga ttttcgtcac ccagcgataa tggcactgaa
     3781 atacccacgc ttaatttccg atctactttc catttatgcg atgtcagcaa gtcgtttttt
     3841 gcccagttaa tggagaatgt tatgcctctc cataccgtac tataactcag gctggtagac
     3901 cgcgtgtttc ctgtgccccg ggaagaatta tacctcgtgg tattgaagtt caaattcccg
     3961 atattaccaa ggggctggga taaggatatc gtagagccat cgtgactatt cttatctctt
     4021 tttctgttcc gttcatcctg tgcacttact gcttcagtca gtgtggcata acctgatgca
     4081 taacggttcg tggttataga caatagactg gccccctgaa tctccagaca accagtatca
     4141 cttat
//
LOCUS       DAZPAB010000262         4090 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZPAB010000262 DAZPAB010000000
VERSION     DAZPAB010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.263"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACHUPG_003853"
     CDS             complement(<1..334)
                     /gene="flhe"
                     /locus_tag="ACHUPG_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFR0863251.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(334..2273)
                     /gene="flhA"
                     /locus_tag="ACHUPG_003854"
                     /pseudo
     CDS             complement(334..2273)
                     /gene="flhA"
                     /locus_tag="ACHUPG_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520726.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2326..3473)
                     /gene="flhB"
                     /locus_tag="ACHUPG_003855"
                     /pseudo
     CDS             complement(2326..3473)
                     /gene="flhB"
                     /locus_tag="ACHUPG_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
     gene            3709..>4090
                     /locus_tag="ACHUPG_003856"
     CDS             3709..>4090
                     /locus_tag="ACHUPG_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFR0863252.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1015 a         1190 c         1003 g          882 t
ORIGIN      
        1 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
       61 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
      121 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
      181 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
      241 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
      301 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
      361 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
      421 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
      481 cgcacccagc atctcctgac gggatagagc ttcctgagtt tgcgccagta aacgatccgc
      541 cagccctggc tccagtcctc ccccgccctg cagcgcctgc gcccgtccca acgccacgcg
      601 cacgacggcg cttaattcat gtggatcgct ttggatgggc gcatgttccg ccagcgtttc
      661 gagaatggtg cgcatatcgc gaatcggtac tttttcatcg aggagatttt gcagcacttt
      721 atgcagtgtg gtgagcgtga cgacgccagg aacgagatct tccgtcagct ttggcatctc
      781 ctgggcgacg cgatccaaca gctgttgcgc ctcctgacga ccaaacagct ctgcggcatg
      841 ctggctaatg aggtggttaa gatgcgttgc taccaccgtg ctggcctcaa ccactgtgta
      901 cccctgaatc tgcgcctgtt cttttagcgc actttcaatc cagatagcat tcaggccaaa
      961 tgccggatcg acggtcgcct caccaggtaa cgtcccggca gcggttccag ggttaatcgc
     1021 cagccagcgc cccggataag catcaccact gccaatctcc acgcctttca tcaaaatgcg
     1081 atagcgggca ggttgcagat ccatattgtc gcgaatgtgc accactggcg gcagaaatcc
     1141 catctcctgg gcaaatttct tgcggatact gcgtatacgg cccaacaact caccatcctg
     1201 ctggaaatcg accatcggga tcagtcgata acccacttcc attcccagag aatcttccag
     1261 ttgtacatcg ttccacgtcg cttcgacaac ggtattattc tctgccattt ttaccggttt
     1321 gggttcggca ggcgcttttt gttcgcgtcc gcgtatccac caggccagcc ccagcaatcc
     1381 ggcagtgaac agcaaaaata ccaggttcgg cattccaggc accaggccga gtaaaccgag
     1441 cacggcggcg cttaacaaca taacgcttgg gttactgaaa agctgattca ccatctgctc
     1501 gccaacatcc tgatcggtgc tgacacgcgt aacgatgccc cccgcggcgg tagaaatcac
     1561 cagcgccgga atttgtgcca ccagaccgtc gccaatggtc aatagcgtat aactttccgc
     1621 cgcgtgtccc atgctcatgc cagttgcagc acgccgacca gcaacccgcc gacaacgtta
     1681 atgaccatga tgaggatccc ggcaatggca tcgccgcgaa caaacttact tgccccgtcc
     1741 attgagccgt aaaaatcggc ttcctgagtc acttcggagc ggcgtttttt cgcctcatct
     1801 tcaccaatca atccagcgtt aaggtcggcg tcaatcgcca tctgcttacc cggcatacca
     1861 tcgagaacaa agcgcgcacc cacttctgct atacgcccgg cacctttggt aatgaccata
     1921 aagttgatga tcacgagaat gacaaacacc acgataccga tagcgaaatt gccaccaacg
     1981 aggaagtgac cgaacgcttc gaccaccttc ccagccgccg ccgcgccggt atgcccttcc
     2041 atcaaaatga tacgggttga agccacgtta agtgccagac gcaacaacat agtaaacaac
     2101 agaatggtcg gaaacgcagc aaactcaagc gtgcgctggg taaacatcgc caccagcaac
     2161 accatgatcg acaaggcaat attgaaggta aacaacaggt cgagtatgaa tgcgggcagt
     2221 ggcagcacca tcatcgacaa gatcaacagg atcaaaatcg gtccggcaag gatctgccat
     2281 tatgtcgatt tcaggtttgc gggcaggcgc agcatcgcgg ccagattact catgggtcgg
     2341 tttctcgtta ataaaatcca gggctgccgg caccggaaga tgagtaggtg tacagggcgc
     2401 tgtccaccag ccagacgcca gcgtttcagt tgccagaccc aggccagcac ttccgccacc
     2461 gcggcgtaca gttgacccgg gatttgttga ccaatctccg catgtcgata cagcgctcgc
     2521 gccagcggcg gcgcttcaag cgtcgggacg ttattttcag cgccaatttc acgaatgcgc
     2581 agcgcgacca gccctgcacc tttagcgacc actttcggtg cgctcatttt gttttcgtca
     2641 tactgcaacg ctaccgaata gtgggtcggg ttattgacaa tgacatccgc tttcggcaca
     2701 tcggccatca tccgacgccg tgcggcagct cgctgcatct gacagatccg tcctttaaca
     2761 tgggggtcgc cttcgctttg tttgaactca tcacgaatat cctgccgcga cattcgcagc
     2821 tttttcaggt ggctgaagat ttggaaaaag acgtcaaatc ccaccattgg aatgacacca
     2881 agcaccacca gcagtgcgca tagccctacc aaatccatcg cattacccat ggcggtaatc
     2941 ggtgactcgg ccattaagcg catcatctgc ggccagtgat gccagagaaa aaaccccgtc
     3001 acgctgccaa ccaggatggt tttcaaaatt gccttaagca actccgcgct agtctgtgcc
     3061 gagaatatcc gtttaatgcc cggtagcggg ttgagtttgg aaaacttcgg ctgcaaggat
     3121 ttgccgctaa ataccagccc ccccagcata accggagaaa tgagcgccac cagcaccacg
     3181 ccgctaatca gcggtagcag cgccagcatg gcttctctga tcagcagaat aatttgcccg
     3241 aggatcagat tcgggtcttt gataatactg tgatcaaaat gcagcccagc ggagagcatg
     3301 cccgacaatc gacgggccag cgacacaccg ccaaaccaga taacactaac gcccaccagc
     3361 aaaatcagca gtgaggtcag ttcacgggaa cgcgggattt gcccctcttc ccgcgctttt
     3421 tctagtcggt gaggtgtggg ggcttctgtt ttgtcgtcgc tctcgtcaga cacgtcgcca
     3481 atcctggaaa gagtattaag cgtaaatgat gccagagcgc aaagcgttca atggtttaag
     3541 taaggggcaa aacaggcggg atttagggcg tttatgcaaa aataggttca gttgcagaac
     3601 gttttgcgaa ccattttcca gttctgatat ttagccattt agcctataga atcgcactcc
     3661 gcctctctgt tagtttgcta ataattaaat tattacaaag gtgatcaaat ggaatggcaa
     3721 caattgctta actgcaaccg tcggaaagat aatctaaaaa aaaccaacat agcattttta
     3781 catccccttg ctagcggaag acaagagatc gagcgggatt acgaccgatt actttttgct
     3841 gcaccaactc gtcggcttgc ggataaaaca caagttttcc cgttagatca aaatgacagc
     3901 atacggacga ggctgactca ttctcacgaa gtagcaaact ttgctcgcgg gataggaatg
     3961 aggctcgctt tcgagatgaa ggagcacatc tttggcgaaa tacccacggg gattgtcgtt
     4021 gaacgcgacg ttcctgctct gttagcagcg atcggactgg cacacgatct cggtaatcca
     4081 ccatttgggc
//
LOCUS       DAZPAB010000263         4088 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZPAB010000263 DAZPAB010000000
VERSION     DAZPAB010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.264"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..1024)
                     /gene="bglB"
                     /locus_tag="ACHUPG_003857"
     CDS             complement(<1..1024)
                     /gene="bglB"
                     /locus_tag="ACHUPG_003857"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR0863253.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1043..2920)
                     /gene="bglF"
                     /locus_tag="ACHUPG_003858"
     CDS             complement(1043..2920)
                     /gene="bglF"
                     /locus_tag="ACHUPG_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR0863254.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3054..3875)
                     /gene="bglG"
                     /locus_tag="ACHUPG_003859"
     CDS             complement(3054..3875)
                     /gene="bglG"
                     /locus_tag="ACHUPG_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR0863255.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
BASE COUNT         1092 a         1067 c          893 g         1036 t
ORIGIN      
        1 atccaaccgg atcaatttgc cacccccact ctgaactttt cagatgcgga ttggggatca
       61 tattcagtat gttgccctgc gcatttttat taatgctttc gtcgtgggaa acacaaccag
      121 tcatgtaata actaaaagag atgaaatcta cagtatgttt taaatcttct gcgtcacttt
      181 cagtcatctc aatggtgata ttgtggtcgc ggaagaaacg ctgcatatag ccgggatatt
      241 ggccacgcgc ctgaacatca ccaaagaaca tccagcgccg gttctcttcc atggcctgca
      301 acatatcctg tggctggcag gtgagggggt aaaccagccc accgagaagc atattgccga
      361 ttttcgcttc ggggatcagg ctatgatagg ctttaactgc ccgcgcactg gcaaccagtt
      421 gatgatggat cgcctgataa acttccgcct cgccactctc ttctgccagc cccacgcccg
      481 tgaatggcgc gtgtaacgac atattgattt cattaaacgt cagccataac gctactttat
      541 gttggtagcg ggtaaagacc gtgcgggcgt aatgctcgaa gtgatcgatg accgcccgat
      601 tagcccaacc gccgtagttt ttcaccagcc catatagcat ttcgtaatgg gataacgtta
      661 ccagcggctt gatccccgcc tgcgccattt catcaaacag ccgatcgtaa aacgctaacc
      721 ccgcttcatt cggttcggct tcgtcgccct gagggaaaat tcgcgcccag gcaatggaaa
      781 tacgcagaca ggtgaagccc atctcggcaa ataacgcgat atcttccggg taacggtaat
      841 aaaaatcgat ggcgacatct ttgatattct ctttccccag gatgcgcggt tccatttttc
      901 ccattacgcc atgaggctgt aaatctgagg tcgtgatccc tttgccatct tccagccagg
      961 caccttccac ctgattggca gctgttgcgc caccccaaag aaatgtttct ggaaatgctt
     1021 tcataattaa ctccttttat cgttagcgaa tgatggataa cagcggttca cctgcgctta
     1081 tctgcgccgt gccgtggggt aatacgtctg taaaatcatc gctattactg attaataccg
     1141 gcgtcgtcag atcaaatccg gcctcgcgaa tagcagggat atcaaaagaa atcagccgat
     1201 cgcctgtatt gaccttgtca cccacgttga cgtgagcgga aaagaatttg ccgtccagtt
     1261 ttacggtgtc gataccgaca tgaatcagga tctccacacc atcatctgac tcaatgccaa
     1321 tggcgtgtaa tgtggcgaac aacgaagcaa ttcgacccgc aaccggagaa cgcacttcac
     1381 caaccgaggg cagaatggcg atacctttac ccaacaggcc actggcaaac gtggtatcag
     1441 cgacgtgaat gagcggcaca atctctcccg tcatcggtga acagataccg ccctgctcag
     1501 gtggtgtaat aacctctggt gttttctctt gcggggcacc ctgcgctggc tgacgtttac
     1561 tggtgatgaa atgaagcatc aacgtaccaa caaatgcgca acctatggca atgacaccgc
     1621 caataacgct ggcccagacg gtgaaatcaa tccccgttga cgggatggtc tgcatgaagg
     1681 tgaaaatact tggcaaacca aaggagtaaa ctttcgtttg cgcgtagcca atgatggtgg
     1741 cccccaatgc tccactgata caggcgataa caaaggggta cttacgcggc aggttgacgc
     1801 catataccgc tggttcggtg ataccaaaca gacccgtcaa cgccgctgat cccgccacca
     1861 cttttttctg cgcatcgcgt tcgcagagga agacgccgag cgccgccccg acctgcgcca
     1921 taatggcggg cattaacagc gggatcatgg tgtcgtagcc cagcacggtg aagttattga
     1981 tacacagcgg caccaggccc ctgtgcagtc cgaacatgac gaagatttgc cagaagccgc
     2041 ccattaccgc gcccgcaaat gcaggaaccg cctgataaag ccagagataa ccggcggcaa
     2101 tcatttcgct tatccaggtt gatagctgcc ccaccagcag aaaggtgacg ggtgtgataa
     2161 ccatcagaca tagcaatggt gtgaagaaat ttttgattgc cgacggtaac cacgcattaa
     2221 gtcggcgttc cagaatgctg cacaaccagg cagaaaaaat aatgggaata accgatgacg
     2281 agtaattcaa caatgtgacc ggaataccca ggaaatccag ccccggcgca tccgcttttt
     2341 gcccgttctc gaaggcagtc agaattaatg gatgcactaa cgctccacca atcaccatgg
     2401 cagtaaatgg attaccgccg aagcgtttgc ccgcggtgta tcccaggatt atcgggaaga
     2461 acccaaacaa ggcatcactg gcgctgaata aaattaaata agtaccactt tgttcggtcg
     2521 tccactgaaa agtgagcgcc agagccagca tacctttcag gatcccggtt gccgccatca
     2581 gaccgatcag aggcgtaaaa atacctgaaa taacataaac aaagcggttt agcagattac
     2641 ctttctcatc attttccggt gcctgttgcg ctttttcgcc aaggcctgcc acgctgttaa
     2701 ccgccaggaa gacatcggcc acatggttac ctatgaccac ctgaaactga ccaccgcttt
     2761 ccaccaccat aataataccg ggggtctttt tcagtacctc agcttgcgct ttgctttcat
     2821 cctttaactt aaaacgtaat cgcgttgcgc aatgcatcag actcacaatg ttatctgcgc
     2881 ccccgactcc tgcgactatt tttctggcta actccgtcat aacttgccct ctaccgcttt
     2941 gcggcaaaac tccaaaaaaa aacctgaaaa aaacggcctg acgtgaatca agcaattttt
     3001 ttcaggtttt gcccgcttag tgcggtaaca atcctttact cagtaataat atttcagtgt
     3061 tctttgcgca cgcgctctat atttatggct aaaaacataa tctctgcggg tgaaatttta
     3121 cgttgatact gcaaaccaat aaaaatggcg atccgttccg cacattgcca tgcttgcggg
     3181 taattttgtt ttactgcttg ttgtaatgat tcatcactat cgttaattga agcatgttca
     3241 agaatacgcc aggataaaaa cttcagatgt gtaaccagtc gctgataact caagctttcc
     3301 tcctggtaat taaggctgaa ctgaaatttt attaattgca gcatttcgcg cattaactgc
     3361 gtgacacctg caacatcctc catatttccg ctcatttggg cactgaccag atgcatggca
     3421 ataaagccca cttcatcttt cggtaactgc acgcccaacc gtttatcaat aatggttaac
     3481 gcttcttccc ctagctggaa ctctttcggg taaagccgct ggatatccca cagcaacacg
     3541 ttttgctgaa agcgtttaat cgcaaactgg caatggtcag ttagcgagat ataaatactg
     3601 tcctgtaatt ttcccaagcg ctcctgcgct aaagagataa gacgatcaca ggttgccatc
     3661 acctcaagag gaatatgact taagagttcg cttaatcgcc cgttcagttc atgactgctc
     3721 aaggcatact ctttttctat tccacttgag ttaattcttt cgccagcgcg tttttgaaag
     3781 ccaattccgc gccccatgac gactttttcc cgctgttgat catcaataac caccacaaca
     3841 ttattgttga gaattttggt gatttgcatg ttcatagcaa ggaccttttt tataaacaaa
     3901 aaaaaaccga cttcaccagt attctctggt tatgtcaggt tttgcctgcg aatgcagtaa
     3961 caatccagtc atttattaat ggtttttata acgaacatcc aggttcggaa attaatttaa
     4021 ttgcgtgcag agaaaatagc aatgcgctat tgataaaaat atgaccatgc tcgcagttat
     4081 taactttg
//
LOCUS       DAZPAB010000264         4078 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZPAB010000264 DAZPAB010000000
VERSION     DAZPAB010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.265"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(243..890)
                     /gene="ychE"
                     /locus_tag="ACHUPG_003860"
     CDS             complement(243..890)
                     /gene="ychE"
                     /locus_tag="ACHUPG_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR0863256.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(921..974)
                     /gene="ychE"
                     /locus_tag="ACHUPG_003861"
     CDS             complement(921..974)
                     /gene="ychE"
                     /locus_tag="ACHUPG_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFR0863257.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1367..4042
                     /gene="adhE"
                     /locus_tag="ACHUPG_003862"
                     /gene_synonym="adhC"
     CDS             1367..4042
                     /gene="adhE"
                     /locus_tag="ACHUPG_003862"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR0863258.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1132 a         1005 c          946 g          995 t
ORIGIN      
        1 ctctaccaaa ggcagtggaa ttactaatat catttcaatt attcttcagc aagcccagga
       61 ttatgatgtg gcgaaaataa catgatggtg aggagaagca gttaaggaaa tccttataac
      121 taactgtaat ttgagaagaa tatttttacc tattcctttt gcttttgata actgcgcggg
      181 aacaaagtaa aaataatgac gaagtaaacg gagcatggca gctccgtttc atttgaaagg
      241 aattaattaa gcaggccggg gaaaatcccc ttaataccag taacgataaa ttcaatcccc
      301 aatgccatca gcaatagccc cataatacgc gtaatcacgt tgatgccggt ctggcgtaaa
      361 acccgtacca gccacggtgc catgcggaac aatccccagc aacataaagc gaacaatgca
      421 atagccacaa agaaaccaaa cagatagcta atgctgtgat aacgcgtacc ccagacgatg
      481 gtagaactga tcgcccctgg ccccgccatc aacggcaacg ccaatggcac cacaccaata
      541 ctttcacgta ccgcggtttc tgatttttct tgcttgttct gtttatcctc gccaagcttg
      601 ccgctgatca tcgacatcgc tatcgtcacc accaggatgc ccccggcgat acggaacgaa
      661 tcaattgata taccaaaaag ttgtagaatc gtgtcgccga gaaaaagcga gatccacaag
      721 ataatggcca cagacaggtt ggctgtaagg ttagttttgt ttcgcgctgc cgctgtctga
      781 taactggtca tgctgataaa gacgggaata atccctaccg ggttgaccag cgcaaataac
      841 ccgatgaaaa atttgaagta aacgggaaaa tcaaaaaggg tctgaatcac ggttcgctcc
      901 gaagcaaaag ccggataatg ttagccataa ataaggttga aaagacgcgc tgacaatacg
      961 ccttttgaca gcatttttca cctcctaact acttaaaatt gctatcattc gttattgtta
     1021 tctagttgtg caaaacatgc taatgtagcc accaaatcat actacaattt attaactgtt
     1081 agctataatg gcgaaaagcg atgctgaaag gtgtcagctt tgcaaaaatt tgatttggat
     1141 cacgtaatca gtacccagaa gtgagtaatc ttgcttacgc cacctggaag tgacgcatta
     1201 gagataataa ctctaatgtt taaactcttt tagtaaatca cagtgagtgt gagcgcgagt
     1261 aagcttttga ttttcatcgg ttaagcaaat catcaccgca ctgactatac tctcgtattc
     1321 gagcagatga tttactaaaa aagtttaaca ttatcaggag agcattatgg ctgttactaa
     1381 tgtcgctgaa cttaacgcac tcgtagagcg tgtaaaaaaa gcccagcgtg aatatgccag
     1441 tttcactcaa gagcaagtag acaaaatctt ccgcgccgcc gctctggctg ctgcagatgc
     1501 tcgaatccca ctcgcgaaaa tggccgttgc cgaatccggc atgggtatcg tcgaagataa
     1561 agtgatcaaa aaccactttg cttctgaata tatctacaac gcctataaag atgaaaaaac
     1621 ctgtggtgtt ctgtctgaag acgacacttt tggtaccatc actatcgctg aaccaatcgg
     1681 tattatttgc ggtatcgttc cgaccactaa cccgacttca actgctatct tcaaatcgct
     1741 gatcagcctg aagacccgta acgccattat cttctccccg cacccgcgtg caaaagatgc
     1801 aaccaacaaa gcggctgata tcgttctgca ggctgctatc gctgccggtg ctccgaaaga
     1861 tctgatcggc tggatcgatc aaccttctgt tgaactgtct aacgcactga tgcaccaccc
     1921 agacatcaac ctgatcctcg cgactggtgg tccgggcatg gttaaagccg catacagctc
     1981 cggtaaacca gctatcggtg taggcgcggg taacactcca gttgttattg atgaaactgc
     2041 tgatatcaaa cgtgcagttg catctgtact gatgtccaaa accttcgaca acggcgtaat
     2101 ctgtgcttct gaacagtctg ttgttgttgt tgactctgtt tatgacgcag tacgtgaacg
     2161 tttcgcaacc cacggcggct atctgttgca gggtaaagag ctgaaagctg ttcaggatgt
     2221 tatcctgaaa aatggtgcgc tgaacgcggc tatcgttggt cagccagcct ataaaattgc
     2281 tgaactggca ggcttctctg taccagaaaa caccaagatt ctgatcggtg aagtgaccgt
     2341 tgttgatgaa agcgaaccgt tcgcacatga aaaactgtcc ccgactctgg caatgtaccg
     2401 cgctaaagat ttcgaagacg cggtagaaaa agcagagaaa ctggttgcta tgggcggtat
     2461 cggtcatacc tcttgcctgt acactgacca ggataaccaa ccggctcgcg tttcttactt
     2521 cggtcagaaa atgaaaacag ctcgtatcct gattaacacc ccggcttctc agggtggtat
     2581 cggtgacctg tataacttca aactcgcacc ttccctgact ctgggttgtg gttcctgggg
     2641 tggtaactcc atctctgaaa acgttggtcc gaaacacctg atcaacaaga aaaccgttgc
     2701 taagcgagct gaaaacatgt tgtggcacaa acttccgaaa tctatctact tccgccgtgg
     2761 ctccctgcca atcgcgctgg atgaagtgat tactgatggc cacaaacgtg cgctcatcgt
     2821 gactgaccgc ttcctgttca acaatggtta tgctgatcag atcacttccg tactgaaagc
     2881 agcaggcgtt gaaactgaag tcttcttcga agtagaagcg gacccgaccc tgagcatcgt
     2941 tcgtaaaggt gcagaactgg caaactcctt caaaccagac gtgattatcg cgctgggtgg
     3001 tggttccccg atggacgccg cgaagatcat gtgggttatg tacgaacatc cggaaactca
     3061 cttcgaagag ctggcgctgc gctttatgga tatccgtaaa cgtatctaca agttcccgaa
     3121 aatgggcgtg aaagcgaaaa tgatcgctgt caccaccact tctggtacag gttctgaagt
     3181 cactccgttt gcggttgtaa ctgacgacgc tactggtcag aaatacccgc tggcggacta
     3241 tgcgctgacc ccggatatgg cgattgtcga cgccaacctg gttatggaca tgccgaagtc
     3301 cctgtgtgct ttcggtggtc tggacgcagt aactcacgcc atggaagctt atgtttctgt
     3361 actggcatct gagttctctg atggtcaggc tctgcaggca ctgaaactgc tgaaagaata
     3421 tctgccagcg tcctaccacg aagggtctaa aaatccggta gcgcgtgaac gtgttcacag
     3481 tgcagcaact atcgcgggta tcgcatttgc gaacgccttc ctgggtgtat gtcactcaat
     3541 ggcgcacaaa ctgggttccc agttccatat tccgcacggt ctggcaaacg ccctgctgat
     3601 ttgtaacgtt attcgctaca atgcgaacga caacccgacc aagcagactg cattcagcca
     3661 gtatgaccgt ccgcaggctc gccgtcgtta tgctgaaatt gccgaccacc tgggtctgag
     3721 cgcaccgggc gaccgtactg ctgctaagat cgagaaactg ctggcatggc tggaaacgct
     3781 gaaagctgaa ctgggtattc cgaaatctat ccgtgaagct ggcgttcagg aagcagactt
     3841 cctggcgaac gtggataaac tgtctgaaga tgcattcgat gaccagtgca ccggcgctaa
     3901 cccgcgttac ccgctgatct ccgagctgaa acagattctg ctggatacct actacggtcg
     3961 tgattatgta gaagatgaaa ctgcagcgaa aaaagaagcc gctccggcta aagctgagaa
     4021 aaaagcgaaa aaatccgctt aatcagtagc gctgtctggc aatataaacg gccccttc
//
LOCUS       DAZPAB010000265         4047 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZPAB010000265 DAZPAB010000000
VERSION     DAZPAB010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.266"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(139..3654)
                     /locus_tag="ACHUPG_003863"
     CDS             complement(139..3654)
                     /locus_tag="ACHUPG_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR0863259.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
BASE COUNT         1045 a         1025 c          843 g         1134 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtca
//
LOCUS       DAZPAB010000266         4042 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZPAB010000266 DAZPAB010000000
VERSION     DAZPAB010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.267"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(126..284)
                     /locus_tag="ACHUPG_003864"
     CDS             complement(126..284)
                     /locus_tag="ACHUPG_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0863260.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWTRWIEI
                     LRVLEVVA"
     gene            complement(886..1077)
                     /locus_tag="ACHUPG_003865"
     CDS             complement(886..1077)
                     /locus_tag="ACHUPG_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR0863261.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1142..1465
                     /locus_tag="ACHUPG_003866"
     CDS             1142..1465
                     /locus_tag="ACHUPG_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFR0863262.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1674..2105
                     /locus_tag="ACHUPG_003867"
                     /pseudo
     CDS             1674..2105
                     /locus_tag="ACHUPG_003867"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="ACHUPG_003868"
     CDS             2143..2673
                     /locus_tag="ACHUPG_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFR0863263.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="ACHUPG_003869"
     CDS             2680..2913
                     /locus_tag="ACHUPG_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFR0863264.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3459..3851)
                     /locus_tag="ACHUPG_003870"
     CDS             complement(3459..3851)
                     /locus_tag="ACHUPG_003870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863265.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          966 a          916 c         1207 g          953 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgtttctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcaga ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct
     3121 gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgtcgct
     3181 gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctcag
     3241 gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg tctctggctg
     3301 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt
     3361 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     3421 gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt gtgagggaat
     3481 ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga ttgggtttcg
     3541 gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat
     3601 tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct tccgtccgat
     3661 ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt
     3721 tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt
     3781 attattctct cttttaataa gctgtttacc acagggttta gttgttgtct aaagcacgca
     3841 gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt
     3901 gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg
     3961 gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct tttttgcggc
     4021 gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAZPAB010000267         3968 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZPAB010000267 DAZPAB010000000
VERSION     DAZPAB010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.268"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..428)
                     /locus_tag="ACHUPG_003871"
     CDS             complement(<1..428)
                     /locus_tag="ACHUPG_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFR0863266.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNY"
     gene            complement(431..886)
                     /locus_tag="ACHUPG_003872"
     CDS             complement(431..886)
                     /locus_tag="ACHUPG_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFR0863267.1"
                     /translation="MIQFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEVRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            complement(886..1734)
                     /locus_tag="ACHUPG_003873"
     CDS             complement(886..1734)
                     /locus_tag="ACHUPG_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFR0863268.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERISQAESTLQEHE
                     TRIAQNESDIASLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGTLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            complement(1734..3152)
                     /locus_tag="ACHUPG_003874"
     CDS             complement(1734..3152)
                     /locus_tag="ACHUPG_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFR0863269.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDGINYGCEQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGCQIRIE"
     gene            complement(3153..3653)
                     /locus_tag="ACHUPG_003875"
     CDS             complement(3153..3653)
                     /locus_tag="ACHUPG_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFR0863270.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDVSPGINVGYIFADADVAPEPGDEHGLSNNAINAVIFNLACRIAPDYALEAP
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGGE"
     gene            complement(3631..3897)
                     /locus_tag="ACHUPG_003876"
     CDS             complement(3631..3897)
                     /locus_tag="ACHUPG_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863271.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFAHPDDL
                     LKDVAEPEPEPEPEPEPEPEPEEKQRKKPGRKPKAAADEPDNEG"
BASE COUNT          914 a         1039 c          972 g         1043 t
ORIGIN      
        1 ttgtaattgc tcatctggtt tgataaccac gactgaccga gagtcggagc aatcgtagcc
       61 agttgattgc ttgtggctgt cgaaccaagt cctcccgtag cctccgcagc agcaagactc
      121 tggtaacgag cctgacctgc aaggtcttta tactgctgag agttgtaata ctgattaagt
      181 gcctgaccct gtccttctaa actggaaagg ttctgaagct ggttaacata ctgctccgca
      241 agcggcgtga acggagcaag gtttttcatg atcgtctgcc actgctgatt ttgcaggtct
      301 gcggcatact tctgagcttc tgctgcatac tttgcacttt tatcagagct gccacctttc
      361 ccgccttttt cagggcaata aggttcctcg ccgcgcagtt ttctgcccag cttaaatgca
      421 tataacatgg ctatctcccg tgattcagga agtcgattag ttcttcgcgt gtagcactgt
      481 aaaatgtcac gtcatccacg cctttgaagt atttcttgat ggttcctact cgcttaaggc
      541 caatcattgc gcagtacatc tgaccgtggc ggaatttgcg cgcagcgaac gatgtgacgc
      601 actgaacggt ggtgttagtc agaatgtatc gccagaacgc cagcccgatt tccttgctga
      661 atccacgaac ctctggcagg tacatggcgt ggcaatcaaa ggtcaaaggc tgaatctcct
      721 gatagtaaac aatgccgccg aactgcccgt gcacgttcac ctcgaagtaa cggcattcag
      781 gcttgtagtc gtatccatca ccgttgttgc tcccggcgat aatgtcgggg tgatttccca
      841 cggcttctat caggtcgatg tttcgcgttg gtttgaactg aatcatcact gctccgcgat
      901 tatcttgatg gttgtggcag taaacgccgc accattcgac tgaatggtta acgtactgcc
      961 atttgtggca agaaatccgt ctttatccac gctgaagaat gtagctaaca ggatgttgtc
     1021 ggttgttgtc gccgcattac gactgctgac cagcgtatca ggaacagagc cggaaaaggt
     1081 tagctgcatt gacctgttgg cggttccgct gggccacgtc ccgacgatcg acagcttgaa
     1141 gaacaaggtt ttgttctcgt tgaacacaac catcttgttg ttaacggtgt cgaagaatgg
     1201 tgccaacgtg ccggatgacg gcgtgagcgt tttcagtagg ctaacaaggt tggtcggcgc
     1261 tgtcgggatg gttacagata cgccagagta aacaacctct gacttcttgc gagtagtggc
     1321 atactccaga gcatcgatgc gcgtttcatg gtctgaaacc tgcgattcca gcgactgaac
     1381 tctggtatca agcgacgcaa tatcgctttc attctgagcg attcgcgttt catgttcctg
     1441 aagagttgat tctgcctggc tgattcgctc ctcatgatta acaagcgttg cttccgcagc
     1501 agaaattcgc tgctcatggt cagcgagaat cacatcctgc tcatcgttcc tgacttgtgc
     1561 gtcataagcg ccctgtccgg cctcgttggc cttgttggcc acgttaccaa catcagtgcc
     1621 ctgtgcgata acgtaaagca gatacgactg cgagaagata ttgcgtggaa ggactgatgt
     1681 gtcgagccgt gtagcctgaa tgattaccgg cacattgaga ttcgaatccg ccattactca
     1741 atccttatct ggcagccaga cagagtgaca ggtgacttcg tgataacgcg caatttgaag
     1801 ccgacatttt tcctgatgcg ccctactcgc ttccacaaaa cgcgtttgtc gtaaacgaac
     1861 ggttcattct gctcaatcat ctgctcacac ccgtaattga tgccgtcagt ggttgcagag
     1921 agaaaaaggc ggtcagcata ctgcgcaacg ccagttgacg attcaacctc aaggtcgaac
     1981 actctggcgt tatccgcttt gaacagtgga gtaaacagca ggtgttcctg ttgcttgtcg
     2041 tactggctgc tgatgtcgaa ttgcaatttc ccggtcacgg actccagctt atcgccgcac
     2101 gttatttgat tgccttcgta aatgaagtcg atagcgcggt acacatcgtc atacaggcct
     2161 gttttcagca cacaccattg cggaccattg gcgcttgaag atgcgtcgta caccagtaca
     2221 tggcgcggaa ggtggataat cagcaactca tgcgcatcaa accgcagcga ttccatcacg
     2281 ccatcagcca gttcatcagc agtgtaggag cggaggattt tctcaatgct cgcgctggcg
     2341 attggtgaca cctgaccgga gccgatgatg tatacagacg gcgcacctgt tgccggattg
     2401 ctgatgaacg cataagaatc agcgaatggt gttttgcagt aagtcccggc aatacctttc
     2461 tgcaccatca gcgatggctg tgcgacatac aaagcggcac caacggtggt tgcacctgtc
     2521 agggaaaaat attcaatcgt cgatgagcca aagcagacga tgaagtctcg ccatgtacct
     2581 atgccgatga tgccgtccgg ctgagactcg gcacgatatt gtgcactgta gcggtcagga
     2641 tgcgattcat cttcaaggtc agtgataaac catgaatcag tgccgtcttt tgaccacgca
     2701 taacgcccac gtaagcgcgt aatgtcgcga actgaaccta actcatactg agtgaatcca
     2761 ctgtctgtag gccagtttga gacggtttta accgtgccat catagcgata ctcgaccagt
     2821 tgaccattaa cgcctaccgc ctgtgatgtc cgaccatgcg ccattgatac gcgaccactt
     2881 ccggcaacat caccgacctc gctttctccc ttatacaact tgccaccgca aacgcgataa
     2941 acagcattct gcgccatgtt gtactcgaca ccgcgcgata caccattcac atcagaacgt
     3001 ttggcaatgc ccgggaatga gcgaagatat ccgctgctgt tgagtatttc tttgggtgta
     3061 gccagcatat tcactggcag atagtcgatg tagtcggcgt ttcggaaatc tttgccgaca
     3121 cctttcataa gcggaagttg ctgaatcggc atttattcgc ccccgttatc gcaaggttcc
     3181 ttccggtgga agtaattcca accattccac ttcgccaact ggttaccgct accaacaggc
     3241 atacggtttg gataaccgga cttacattta gcggcttttg ctctgtccat tgcagacagt
     3301 ttgacgagtc gctctttccc gtatctggca gtggttataa gttttgctgg cgcttccagc
     3361 gcataatctg gagcaatgcg gcaggcaagg ttgaaaatga cggcattgat agcgttattt
     3421 gataaaccgt gctcatcgcc cggttctgga gcgacatctg catcagcgaa aatgtagcca
     3481 acgttgatac caggtgacac atcaccgcca agccattcag ccatcatcat ttcaaggtcg
     3541 ttgacgccgt cttccataga ctgcggttct acatcggtta acgtggcatt tgatgccaca
     3601 ccgagcttac gtaatgccgc aagaactaaa tcacccttcg ttgtcaggtt catctgctgc
     3661 cgccttaggt tttcgaccag gctttttacg ctgtttttct tctggctctg gctctggctc
     3721 tggctctggc tctggctctg gctctggctc tgcaacatcc ttcagaaggt catcgggatg
     3781 tgcaaaccag ccagcatcca gatattcctg aagctcttcg gctttcacga tttcaaagtc
     3841 gtatccaacg cctttccact tcttcatgtc gccatgacga aagatcatgt gtgtcatgtt
     3901 tgtctccaga taaaaaaggg agccgaagct ccctctggtt atcacgcagt ctggttaggc
     3961 agaccaac
//
LOCUS       DAZPAB010000268         3933 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZPAB010000268 DAZPAB010000000
VERSION     DAZPAB010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.269"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="ACHUPG_003877"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="ACHUPG_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR0863272.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="ACHUPG_003878"
     CDS             1828..3147
                     /locus_tag="ACHUPG_003878"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR0863273.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3524..>3933
                     /gene="dosC"
                     /locus_tag="ACHUPG_003879"
     CDS             3524..>3933
                     /gene="dosC"
                     /locus_tag="ACHUPG_003879"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFR0863274.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQV"
BASE COUNT          991 a          944 c          942 g         1056 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt cta
//
LOCUS       DAZPAB010000269         3931 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZPAB010000269 DAZPAB010000000
VERSION     DAZPAB010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.270"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            72..302
                     /locus_tag="ACHUPG_003880"
     CDS             72..302
                     /locus_tag="ACHUPG_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863275.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            280..780
                     /locus_tag="ACHUPG_003881"
     CDS             280..780
                     /locus_tag="ACHUPG_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFR0863276.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            780..2198
                     /locus_tag="ACHUPG_003882"
     CDS             780..2198
                     /locus_tag="ACHUPG_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFR0863277.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            2198..3046
                     /locus_tag="ACHUPG_003883"
     CDS             2198..3046
                     /locus_tag="ACHUPG_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFR0863278.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            3046..3501
                     /locus_tag="ACHUPG_003884"
     CDS             3046..3501
                     /locus_tag="ACHUPG_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFR0863279.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            3504..>3931
                     /locus_tag="ACHUPG_003885"
     CDS             3504..>3931
                     /locus_tag="ACHUPG_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFR0863280.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNY"
BASE COUNT         1034 a          957 c         1013 g          927 t
ORIGIN      
        1 gttggtctgc ctaaccagac cgcgtgataa ccagagggag cttcggctcc cgtttttatc
       61 tggagacaag catgacacac atgatctttc gtcatggcga catgaagaag tggaaaggcg
      121 ttggctacga ctttgaaatc gtgaaagccg aagagcttca ggaatatctg gatgctggct
      181 ggttttcaca tcctgatgac cttttgaagg atgttgcaga gccagagcca gaagaaaaac
      241 agcgtaaaaa gcctggtcga aaacctaagg cggcagcaga tgaacctgac aacgaagggt
      301 gatttagttc ttgcggcatt acgtaagctc ggtgttgcat caaatgccac gttaaccgat
      361 gtcgaaccgc agtctatgga agacggcgtc aacgaccttg aaatgatgat ggctgaatgg
      421 cttggcggtg atgcgtcacc tggtatcaac gttggctaca tttttgctga tgcagatgtt
      481 gctccggatc cgggcgatga gcacggttta tcaaataacg ctatcaatgc cgtcattttc
      541 aaccttgcct gccgaattgc tccagattat gcgctggaag cgtctgcaaa acttataacc
      601 actgccagat acgggaaaga gcgactcgtc aaactgtctg caatggacag ggcaaaagca
      661 gctaaatgta agtccggtta tccaaaccgt atgcctgttg gtagtggtaa ccagttggcg
      721 aagtggaatg gttggaatta cttccaccga aaggaacctt gcgataacgg gagcgaataa
      781 tgcctattca gcaacttccg cttatgaaag gtgtcggcaa agacttccga aacgccgact
      841 atatcgacta tctgccagtg aatatgctgg ctacacccaa agaaatcctg aacagcagcg
      901 gatatcttcg ctcattcccg ggcattgcca aacgttctga cgtgaacggt gtatctcgcg
      961 gcgtcgagta caacatggcg cagaatgctg tttatcgcgt gtgtggcggc aagctgtaca
     1021 aaggagaaag tgaagtcggt gatgttgccg gaagtggtcg tgtatcaatg gcgcatggtc
     1081 ggacatcaca ggcggtaggc gttaatggtc aactggtcga gtatcgctat gatggcacgg
     1141 tcaaaaccgt ctcaaactgg cctacagaca gtggattcac tcagtatgag ttaggttcag
     1201 ttcgcgacat tacgcgctta cgtgggcgtt atgcgtggtc aaaagacgga actgattcat
     1261 ggtttatcac tgaccttgaa gacgaatctc atcctgaccg ttacagcgca caatatcgtg
     1321 ccgagtctca gcctgacggc atcatcggta tcgggacatg gcgagacttc atcgtctgct
     1381 ttggttcatc gactattgaa tatttctccc tgactggcgc aaccaccgtt ggtgccgctt
     1441 tgtatgttgc acaaccatcg ctgatggtgc agaaaggcat tgccggaact tactgcaaaa
     1501 cgccattcgc tgattcgtat gcgtttatca gcaatccggc aacgggtgcg ccgtctgtgt
     1561 atatcatcga ctccggtcag gtgtcaccaa ttgccagcgc gagcattgag aaaatcctcc
     1621 gctcctacac tgctgatgaa ctggctgatg gtgtgatgga atcgctgcga tttgatgctc
     1681 atgagttgct gattatccac cttccgcgtc acgtcctcgt gtacgacgca tcttcaatcg
     1741 ccaatggccc acaatggtgt gtgctgaaaa caggtctgta tgacgatgtg taccgcgcta
     1801 tcgacttcgt ttacgaaggc aatcagataa cgtgcggcga taagctggaa tctgttaccg
     1861 ggaaactgca attcgatatc agcagccagt acgacaagca acaggaacac ctgctgttta
     1921 ctccactctt caaagcggat aacgccagag tgttcgacct tgaggttgaa tcgtcaactg
     1981 gcgttgcgca gtacgccgac cgcctttttc tctctgcaac cactgactgc atcaattacg
     2041 ggcgtgagca gatgattgag cagaatgaac cgttcgttta cgacaagcgt gttttgtgga
     2101 agcgagtagg gcgcatcagg aaaaatgtcg gcttcaaatt gcgcgttatc acgaagtcac
     2161 ctgtcactct gtctggcgct cagataagga ttgagtaatg gctgattcga atctcaatgt
     2221 gccggtaatc atccaagcta cgcggctcga tacatcagtc cttccacgca atatcttctc
     2281 gcagtcgtat ctgctttacg ttatcgcaca gggcactgat gttggtaacg tggctaacaa
     2341 agccaacgag gccggacagg gcgcttatga tgcacaggtc aggaacgatg agcaggatgt
     2401 gattctcgct gaccatgagc agcgaatttc tgctgcggaa gcaacgcttg ttaatcatga
     2461 ggagcgaatc agacaggcag aatcaactct tcaggaccat gaaacacgaa tagctcagaa
     2521 tgaaagcgat atttcgtcgc ttgacacaag agttcagtcg ctggagtcgc aggtttcaga
     2581 ccatgaaacg cgcatcgatg ctctggagta tgcaaccaca cgcaagaagt cagaggttgt
     2641 ttactctggt gtatctgtaa ccatcccgac agcgccgacc aaccttgtta gcctgctgaa
     2701 aacgctcaca ccgtcatccg gctcgttggc accattcttc gacaccgtta acaacaagat
     2761 ggttgtgttc aacgagaaca aaaccctgct cttcaagttg tcgattgtcg gcacgtggcc
     2821 cagcggaacc gccaacaggt caatgcagct aaccttttcc ggctctgttc ctgacacact
     2881 ggtcagcagt cgtaatgcgg cgacaacaac cgacaacatc ctgttagcta cgttcttcag
     2941 cgtggataaa gacggctttc ttgccacaaa tggcagtacg ttaaccattc agtcgaatgg
     3001 tgcggcgttt actgccacaa ccatcaagat aatcgcggag cagtgatgat tacattcaaa
     3061 ccaacgcgaa acatcgacct gatagaagca gtcggaaatc accctgacat tatcgccggg
     3121 agcaacaacg gtgatggata cgactacaaa cctgaatgcc gttactttga ggtgaacgtg
     3181 cacggtcagt ttggcggcat tgtttactat caggagattc agccgctgac attagattgc
     3241 cacgccatgt acctgccaga gattcgcggc ttcagcaagg aaattgggct ggcgttctgg
     3301 cgatacattc tgactaacac caccgttcag tgcgtaacat cgttcgctgc acgcaaattc
     3361 cgacacggtc agatgtactg cgcaatgatt ggccttaagc gtgtaggaac catcaagaaa
     3421 tacttcaaag gcgtggatga cgtgactttt tacagcgcca cacgcgaaga actaatcgac
     3481 ttcctgaatc acgggagata gccatgttat atgcatttaa gctgggcaga aaactgcgcg
     3541 gcgaggaacc ttattgccct gaaaaaggcg ggaaaggtgg cagttctgat aaaagcgcaa
     3601 agtatgcagc agaagcccag aagtatgctg cagacctgca aaatcagcag tggcagacga
     3661 tcatgaaaaa ccttgctccg ttcacgcctc ttgcggagca gtatgttaac cagcttcaga
     3721 acctttccag tttagaaggt caggggcagg cacttaatca gtattacaac tctaagcagt
     3781 ataaagacct tgcagggcag gcgcgttacc agagtcttgc tgctgcggag gcgacgggtg
     3841 gacttggttc gacagccaca agcaatcaac tggctacgat cgcgccgaca ctcggtcagt
     3901 cttggttatc aaatcagatg agcaattaca a
//
LOCUS       DAZPAB010000270         3904 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZPAB010000270 DAZPAB010000000
VERSION     DAZPAB010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.271"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(131..1480)
                     /gene="lysC"
                     /locus_tag="ACHUPG_003886"
     CDS             complement(131..1480)
                     /gene="lysC"
                     /locus_tag="ACHUPG_003886"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR0863281.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     regulatory      complement(1572..1768)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2005..3654
                     /gene="pgi"
                     /locus_tag="ACHUPG_003887"
     CDS             2005..3654
                     /gene="pgi"
                     /locus_tag="ACHUPG_003887"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR0863282.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT          989 a         1036 c          981 g          898 t
ORIGIN      
        1 ttttctgctc attattagct ccttctgatg ccattctatt tcaggaagga gtgtccgtta
       61 aactcaggct acctcatagt gacaagaaaa tcaatacggc ccgaaatata gcttccaggc
      121 catacagtat ttactcaaac aaattactat gcagtttttg caccacctgc tcggcatctt
      181 cgccgggcac caggaagcac aggttatggc tggatgcgcc gtaacaaatc atgcgaatgt
      241 tgaacggttc cagtacgccg aatacctctt tgccaacgcc gcaggctttt gacaggtcat
      301 tgccaatcaa cgcgaccagc gccagacctt cttccacctc cacccggcac agtgcggaaa
      361 gctccatcag cagagattgc gtcagcaacg tatcgccagt ggaggttgaa ccggtggtat
      421 caagggttaa tgccacgctc acttctgacg tggtgattaa gtctaccgaa atattatgcc
      481 gcgcgaggat gccgaaaact tccgcgagga agccgcgaga atgcagcata ttcaggctgt
      541 gcaaagtgag cagagtctga ttgcgacgaa gcgccagcgc gcggaacagc ggcggatttt
      601 cagttttatt gcacaccagc gtaccacctg cgcgtgggtc tttgctggag ccgacaaaga
      661 ccgggatatc gctgcgtact gcgggtagca acgttgccgg atgcagtact tttgcaccaa
      721 aagttgccat ctctgccgct tcggcaaacg cgatttcatc aatgcgtttt gctgcggaaa
      781 ctacgcgtgg atcggtggtg tagatgcccg ggacgtcggt ccagatatca acacgagatg
      841 cgtgtaaagc ctccgccagc aaggctgccg tataatcgct gcctccacgg ccaagcgtcg
      901 ttgtacgacc tttattttcg ctaccgataa atccctgggt gatcactaag ccttcattga
      961 ggcgtgggag cagctgcagc gcggccagtt ccgccagcgc ggctacatct ggctctgcac
     1021 gaccaaatcg gtcgttggta cgcatcactt tacgtacatc aaaccactgt gcctgaacat
     1081 cgcgttcgcg caggatttcg acaaacagca gggtcgacat cagctcgccg tggctgacca
     1141 gctcatctgt cagcgccgga gacgttgcca gcgccgccgc ttctgccaga acagtaatgt
     1201 tctccagcag acgttcaatc tcttcacgga taacgttcgg gtaacgcaga cgttccagaa
     1261 tggcaaactg gatgttgcga atagcgtcga gtttttcgaa tcgctcgcca ggttccagcc
     1321 cttccgctaa agcgaccagc agattagtga taccagcaga agccgagagg actactaaac
     1381 gcacgttggc atcagaaagc acaatatcag cgctgcggtt catggcgtca aaatcagcta
     1441 cgctggtacc gccaaatttg gagacaacaa tttcagacat aactacctcg tgtcagggga
     1501 tccattttca gccttggcac aagggaagag cggaagacgg gtgggcgcag agcgatactt
     1561 cgctactatt ttcacccaga agtgctccac cacttgcgaa acgcccgact gcgaacgctt
     1621 ctggtgacaa cccaggggat tcagcccctg tagccgatga tgaacgtggc cagccgttca
     1681 atcacctcgg cgatgcaccc cctcaggtgt tatcacagga ctggctcctc caacaccgtt
     1741 acttgggcaa cgcgcctctt ctggcctgcg ctagcgcagg tagtacattt ataaataaag
     1801 ggtgagcggg gcggttgtca acgacggggt catgcggatt tttcatccac tcctggcggt
     1861 cagtagttca gctaataaat gcttcactgc gctaagggtt tacactcaac attacactaa
     1921 cggcactaaa accatcacat ttttctgtga ctggcgctac aatcttccaa agtcacaatt
     1981 ctcaaaatca gaagagtatt gctaatgaaa aacatcaatc caacgcagac cgctgcctgg
     2041 caggcactac agaaacactt cgatgaaatg aaagacgtta cgatcgccga tctttttgct
     2101 aaagacggcg atcgtttttc taagttctcc gcaaccttcg acgatcagat gctggtggat
     2161 tactccaaaa accgcatcac tgaagagacg ctggcgaaat tacaggatct ggcgaaagag
     2221 tgcgatctgg cgggcgcgat taagtcgatg ttctctggcg agaagatcaa ccgcactgaa
     2281 aaccgcgccg tgctgcacgt agcgctgcgt aatcgtagca ataccccgat tctggttgat
     2341 ggcaaagatg ttatgccaga agtcaacgcg gtgctggaga agatgaaaac cttctcggaa
     2401 gcgattatct ccggtgagtg gaagggttat accggcaaag caatcactga cgtagtgaac
     2461 atcgggatcg gcggttctga cctcggcccg tacatggtga ccgaagcact gcgtccgtac
     2521 aaaaaccacc tgaacatgca ctttgtttct aacgtcgatg ggactcacat ctcggaagtg
     2581 ctgaaaaaag taaacccgga aaccacgctg ttcctggtag catctaaaac cttcaccact
     2641 caggaaacca tgaccaacgc ccatagcgcg cgtgactggt tcctgaaagc ggcaggtgat
     2701 gaaaaacacg ttgcgaaaca ttttgcggcg ctctcaacca atgccaaagc cgttggcgag
     2761 tttggtattg atactgccaa catgttcgag ttctgggact gggtcggcgg ccgttactct
     2821 ttgtggtcag cgattggcct gtcgattgtt ctctccatcg gctttgataa cttcgttgaa
     2881 ctgctttctg gcgcacacgc aatggacaag catttctcca ccacgcctgc cgagaaaaac
     2941 ctgcctgtcc tgctggcgct gattggcatc tggtacaaca atttctttgg tgcggaaact
     3001 gaagcgattc tgccgtatga ccagtatatg caccgtttcg cggcgtactt ccagcagggc
     3061 aatatggagt ccaacggtaa gtatgttgac cgtaacggta acgttgtgga ttaccagact
     3121 ggcccgatta tctggggtga accaggcact aacggtcagc acgcgttcta ccagctgatc
     3181 caccagggaa ccaaaatggt accgtgcgat ttcatcgccc cggctatcac ccataacccg
     3241 ctctctgatc accaccagaa actgctgtct aacttcttcg cccagaccga agcgctggcg
     3301 tttggtaaat cccgcgaagt ggttgagcag gaatatcgcg atcagggtaa agatccggca
     3361 acgcttgact acgtggtgcc gttcaaagtg ttcgaaggta accgcccgac caactccatc
     3421 ctgctgcgcg aaatcactcc gttcagcctg ggtgcgttga ttgcgctgta tgagcacaaa
     3481 atctttactc agggcgtgat cctgaacatc ttcaccttcg accagtgggg cgtggagctg
     3541 ggtaaacaac tggcaaaccg tattctgcca gagctgaaag atgataaaga aatcagcagc
     3601 cacgatagct cgaccaatgg tctgattaac cgctataaag cgtggcgcgg ttaatcatca
     3661 tgcgtgcctg atgccgatat gtaggccgga taaggcgttt acgccgcatc cggcaaccgg
     3721 agcctgatgc caatatcgac attaatgcat agtatgagga gttaaataac cttaaaaaca
     3781 atgccgacaa taagtcggca ttgttttatc agataaatcc ccttgtctgt gatttaacgg
     3841 aaatcatacc gtgaggttaa tcctaaaata gatttttaat cgttgtttat ttcggagaat
     3901 acgc
//
LOCUS       DAZPAB010000271         3895 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZPAB010000271 DAZPAB010000000
VERSION     DAZPAB010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.272"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..225
                     /locus_tag="ACHUPG_003888"
     CDS             <1..225
                     /locus_tag="ACHUPG_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFR0863283.1"
                     /translation="GILSQRGGLEIQSQFLRGNEQVYQCQVAFVLPGERVMMAFTYAR
                     TTPLTPADMTRWAEIKKNLRFRMRQEVRTN"
     gene            244..3630
                     /locus_tag="ACHUPG_003889"
     CDS             244..3630
                     /locus_tag="ACHUPG_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR0863284.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDAPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            3633..>3895
                     /locus_tag="ACHUPG_003890"
     CDS             3633..>3895
                     /locus_tag="ACHUPG_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863285.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYD"
BASE COUNT          960 a          916 c         1232 g          787 t
ORIGIN      
        1 ggaatattat ctcaacgagg cggtttggaa atacagagcc agtttctccg gggtaacgag
       61 caggtgtacc agtgtcaggt ggcatttgta ttgccgggtg agcgggtcat gatggccttc
      121 acctacgcca gaaccacacc gctgaccccg gcagatatga cgcgctgggc tgaaattaaa
      181 aagaatctgc gcttcaggat gcggcaggaa gtcaggacga attaatcata cagggaacgg
      241 gacatggaag aggcattctg ggcggcaaga cagggtgatg ccctgctgca tacgtcgttt
      301 atggcagatg tgctgggggc ggtgctggag gtggccgcaa atgtcgttat tgatgccctc
      361 attgtcggca caacctcgtt actggcgacg tcagtgggga tcacgatggg gtgcacggcc
      421 gtcgtgctga ccggttttgt ggcgggtgtg gcgatggtgt acaccggcgt gacagaaaag
      481 gtcagcgaag cctgttcagc cctcgcaaat atgcttttcc cgcctcagat tgaaggttac
      541 atcctcaccg gctctggtga cacatggatc aacagcaaac ctgccgcgcg ggcagcggcg
      601 actgcggcat cgcggcagga catagaggcg caggaagcgc aggcgaaggc agaacaggaa
      661 gaagcgcagc gtcaggagga tgccagaacg ttccgggatg tggccgggga atatctggaa
      721 atggcggggg ccatagccct ggcggttaac ccggtgacag ggcctgtgct gatgtcccgg
      781 gctctgcacg accagctcag tacggaagaa gggcgttctg aaatggtgga tggcgtgaca
      841 cattttgtgt cggaactatg gcagcccact gtggccagtg cggccccggg atccaccccg
      901 acccctgacg ataaaatcga ctgccataaa catccttcct cactgacgca gtttctcgca
      961 caaaaaaaag ccgcctttct ggatgccccc ctgggaacgg cccttaacct gctgaatccg
     1021 ggcggtatgc tggaaaccgc ctttcaggga gtgaatgcgg ttattggcag catcagtaat
     1081 ctgttcaaag gcgatgacga gccgcccgca gcggaatata tcgcggaagg cgcgcgggat
     1141 gtgcgtatca acagccagcc ggcgacacgc agcggggcac gctgtacctg cgaggccagg
     1201 gtggtgaatg agccggggaa cggggcgttt gtgtcaccgg atgtgcgcat tggcggtccg
     1261 ttactggtgg tcagggatat cagaagcggc aggtcgcaga taaccctggt ggcaaccgtg
     1321 gcgctgatgt ttcttcgccc gggcaaaatg ctttctaaaa tcgcctgttt tgctgccagt
     1381 gtcgggatga gcatgctgac gcagaaggcc accagtgccc ttccgcatcc ggtcaatgcc
     1441 gccaccgggg ccaaatacct ggccgatgac gatgacttcg atttcagtct gccgggccac
     1501 tttccgctgg actggcagcg cgtgtacagc agtcgtgacg aacgtacgga aggaatgttt
     1561 ggtcagggct ggagtgtaat gtatgaagtc agcctcgtgt gcacaccggg tagcgcagac
     1621 gaaaactgca tgacctttgt gtcgggcatg gggcgacgct tggatatgga agccgtactg
     1681 ccggggggcg ggttttacag ccccggtgaa gggctggcgg taaggcgtgg tgagcagggg
     1741 cactggctta tcagcagcga tgacgggcag tgttttctgt ttgaggctga cccgcaccac
     1801 ccgcagcgcc agcggctgaa aatgctgggc gaccgtaaca gcaactgtct gaatctgtac
     1861 tacgacgacc ggggccgcat tgtggaaatc agcggcgaac agcagcgtcc gtgcatccgt
     1921 ctgtattacg aactggcggc gcatccccgg cgggtgacac agatttatca gcatttccct
     1981 gagactgcac ctttactgct gcgtcgttac agctatgacg aggccggcca cctgaacggc
     2041 gtgtacgaca gcacggggca cctgctgcgt gagtttgctt acgacgaaaa ccactgcatg
     2101 acgctgcacc gccagccggg cggcgaaggg tattattacc agtggggctg gtatgaaggt
     2161 ccggatgacg cggcatggcg ggtgacgggg caccacacgg acagcggtga acagtaccgg
     2221 ctggcgtggg acctggcgca gcgcaggctg tgcgtgacgg acggactggg gcgcacacgt
     2281 tatcaccagt gggatgcgca gaatcaggtg acggcgtacc aggacgaagc cggacaggtg
     2341 acgacattcc gctggagcga cgaagaacga ctgttgctgg ggatgaccga ccctcagggc
     2401 ggcaaatggc gctatgtgta tgacctgcag gggcatatca cggagacgca cgacccgctg
     2461 gggcgggtgg agcaggcgca gtggcacccg gtgtggcacc agccggaaac ggaggtggac
     2521 gcggcgggaa acctgctggg gaggcgcgcg ggggaaaggg ccacggcaga aaacagcgtg
     2581 gtgccgttca accggctgat gtcttaccgt ggtgtgcact accgttatga cgaatttggc
     2641 cgtgcggtgg aaaaggaggg ccggagcgga acgcagagtt accgttatga tgcggaacac
     2701 cggatggtgg aagtgacgac agcgcggggg acataccggt atgtgtacga tgcactgggg
     2761 aggcggacgg aaaaacagca catcagccct gacggtaagc cgtacaaccg gacaaaattt
     2821 ttatgggacg gaatgcggct ggcgcaggag agcaggccgg agggaacagg cagcctgtac
     2881 atctacaggg accaggggag ttatgaaccg ctggcgcggg tggataaggc gggcaaagag
     2941 ggcccgaacc ggatactgta ttttcacacg gatgtgaacg gcgcgccgga agagatgacg
     3001 gacagcgacg ggaaaatcgt gtgggagacg gggtatcagg tctggggtaa cacgatacag
     3061 gagaaggacc acggcggggt ggagcagaac ctgcgctacc agggacagta cctcgacagg
     3121 gaaacggggc tgcactacaa tctgcacagg tactatgatc cggatgtcgg gcggttcatg
     3181 gtaactgacc cgatagggtt gagaggcggt ttaaatctct acagttatgc acctaatcct
     3241 ctaaaatatg ctgatccact tggattaact ccatgtgctg ttagtaacca aaaagcgaat
     3301 cgtttattag atagtagtga aaccaaagtt actgttaggt ctagaagtga cgctgaacaa
     3361 ttgtttatgg atagatattt aggtcataat tataaaaata tgacaggtga gtcaggcccc
     3421 agcactaaga acttaatgga atatcttact gaaaataaaa caaaagcagg ttcatatcat
     3481 tgggatgata taaaagatcc ttctgtaaca aaaccttcat atagagtgtc aggtcatggg
     3541 ccaggaaatc ctgatggtga tttaccacat ttacaagttc atcaacatgg tggaagtgtt
     3601 agacatatat ttttcccgtg ggagacataa taatgaacaa tctcaataca ttattaagtg
     3661 gaaaggtttt gctcaaagaa attagtccat taatgcctgg atatagaaca tggattgaaa
     3721 tttcattgat cgatgaatta aaaccatctt atccttttag attagatgaa tatgctatga
     3781 tagggcattc accttatgcc aatgaatgta ataaagatga agctaaattt aaactacgaa
     3841 tatcatcatt tttagcttct gacatagata atgaatacga tccatcatat gatta
//
LOCUS       DAZPAB010000272         3831 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZPAB010000272 DAZPAB010000000
VERSION     DAZPAB010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.273"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            71..1405
                     /gene="adeQ"
                     /locus_tag="ACHUPG_003891"
                     /gene_synonym="yicO"
     CDS             71..1405
                     /gene="adeQ"
                     /locus_tag="ACHUPG_003891"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFR0863286.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1458..1910
                     /locus_tag="ACHUPG_003892"
     CDS             1458..1910
                     /locus_tag="ACHUPG_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFR0863287.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2121..3311
                     /gene="nepI"
                     /locus_tag="ACHUPG_003893"
     CDS             2121..3311
                     /gene="nepI"
                     /locus_tag="ACHUPG_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFR0863288.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3352..3645)
                     /gene="yicS"
                     /locus_tag="ACHUPG_003894"
     CDS             complement(3352..3645)
                     /gene="yicS"
                     /locus_tag="ACHUPG_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFR0863289.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
BASE COUNT          780 a          860 c         1016 g         1175 t
ORIGIN      
        1 tatcactgaa ccgttgttgt catttgtttt acaaaagcaa gggattaaat ctcctccaat
       61 ggacaaaaaa atgaataatg acaataccga ttacgtgagt aatgaatcag ggacgctttc
      121 gcgattattt aaactacctc agcatgggac caccgtccgc acagaattga ttgcggggat
      181 gaccactttt ttaaccatgg tgtacattgt ttttgtgaac ccgcaaatcc tcggcgcggc
      241 acaaatggac ccgaaagtgg tgtttgttac cacctgtttg attgccggta tcggcagtat
      301 tgcgatgggg atatttgcta acttacccgt ggcgctggct ccggcaatgg ggctgaacgc
      361 cttctttgcg ttcgtggtcg tgggggcgat ggggatctcc tggcagaccg ggatgggcgc
      421 gatattctgg ggcgcaattg ggctattttt gctcacgctg tttcgtatcc ggtactggat
      481 gatctccaac attccattaa gtttacgtat tggtatcacc agtggaatcg gattatttat
      541 tgccttaatg ggattaaaaa acactggtgt tattgtcgcc aataaagaca cgctggtgat
      601 gattggcgat ttaagttctc acggcgtgtt gttaggtatt ttagggtttt ttattataac
      661 cgtgttgtca tcacgtcatt ttcatgccgc ggtgctggtt tctattgtgg tgacgtcttg
      721 ctgtggatta tttttcggtg atgttcattt tagcggcgtc tattccattc cgcctgatat
      781 tagcggcgtc attggtgaag tagatttgag cggcgcgtta acacttgaac tcgccggtat
      841 cattttctcc tttatgctga tcaacctatt tgattcatca ggaacattaa ttggtgtaac
      901 tgataaagcg ggcttaatag atagcaacgg taaattcccc aatatgaata aggcgctgta
      961 tgttgatagc gtcagttcgg tggcaggcgc atttatcggt acctcgtctg ttactgccta
     1021 tattgaaagt acttctggtg tggcagtcgg tggtcgcacg gggctaacgg ctgtcgtggt
     1081 cggcgttatg ttcctgttag ttatgttctt ctcaccgctg gtggcgatgg ttcctcctta
     1141 cgcaaccgct ggagcgttaa tctttgttgg cgtgctgatg acttcgagcc tggcgcgcgt
     1201 taactgggat gattttaccg aatcggtgcc tgcgtttatt accacggtga tgatgccctt
     1261 tactttctcg atcaccgaag ggattgcact cggctttatg tcgtactgca tcatgaaagt
     1321 gtgcaccggg cgctggcgcg atctgaacct gtgtgtggtg gtggtcgcag ctctgttcgc
     1381 attgaagatt attctggtgg attagtaact ggcgagggtt tccagatatc atgagttctg
     1441 attacgcagg agaactcatg atctggataa tgctcgccac gctggcggta gtgtttgtgg
     1501 ttggttttcg ggtgctgaca tccggggcca gaaaagcgat tcgccgtctc agcgatcggc
     1561 tgaatatcga tgtcgtaccc gtggagtcga tggtcgatca aatgggaaag tcagccggtg
     1621 acgaattttt acgttatttg catcgcccgg atgagtcgca cctgcaaaac gccgcgcagg
     1681 tgttgcttat ctggcaaatt gtcattgtcg atgatagcga acagaacctg ctgcaatggc
     1741 accggatttt acaaaaagcc cgcctttccg cgccgattac cgatgctcag gtcaggctgg
     1801 cgctgggttt tctgcgtgaa accgaacctg aaatgcagga tattaatgct tttcagatgc
     1861 gctataacgc gttctttcag cctgccgagg gcgttcactg gttgcattga aaccgtttcg
     1921 tcttatcagc ctacagaccg cgcctgacct gtcgggtgga taagacgttc acgccgcatc
     1981 cggtaatctt tgccctgttc cgtttcgatc ttaactccag ctcaagcaac accgtaaaag
     2041 ttgcaaaaac gttaaattcg tcacacattt caatgttaaa gtggtcggct tttcccctga
     2101 aacatgccac gggtaacacc atgagtgaat ttattgccga aaaccgcggc gcggatgcca
     2161 tcacccgacc gaactggtca gccgttttct cggtggcgtt ttgtgtcgcc tgtctgatta
     2221 tcgttgagtt tttgcccgtc agtttgttga cgccaatggc ccaggattta ggcatttcgg
     2281 aaggggttgc cgggcaatcg gtgaccgtga ccgcctttgt ggcaatgttt gccagtttgt
     2341 ttattaccca gacaattcag gccactgacc gccgctacgt tgttattttg tttgccgttt
     2401 tgctgacgct ctcctgcttg ctggtttcct ttgctaactc attcagtttg cttttaatcg
     2461 gtcgtgcctg tctggggctg gcgctgggcg ggttctgggc gatgtcggcg tcgctgacca
     2521 tgcgtctggt accgccgcgt acggtgccga aggcgctgtc ggtgatcttc ggcgcggttt
     2581 ctattgcgct ggtgattgcc gcgccgttgg gcagtttttt aggcgagctt atcggttggc
     2641 gcaatgtctt taatgcggcg gcggtgatgg gcgtgctgtg tattttctgg attatcaaat
     2701 cattgccttc actgccaggc gaaccctcgc atcagaaaca aaatactttc cgcttattac
     2761 aacgtccggg tgtgatggca gggatgatcg ccatcttcat gtctttcgcc gggcagtttg
     2821 ctttcttcac gtatattcgc ccggtgtata tgaacctggc gggattcggc gtggatggct
     2881 taacgctggt gctgttgagt tttggtatcg ccagctttat tggtacgtcg ctttcgtcgt
     2941 tcattcttaa acgttcggta aaactggcct tagcaggcgc gccgttaata ctggctgtga
     3001 gtgcgttggt actgacgttg tggggaagcg ataaaatcgt tgctaccggc gtggcgatta
     3061 tctgggggct aacttttgca ttggttcccg tcggctggtc aacgtggatc acccgctcgc
     3121 tggccgatca ggcagaaaaa gccgggtcta ttcaggtggc ggttattcag cttgctaata
     3181 cctgtggcgc ggcaatcggc ggttatgcgc tggataatat tggtctgact tcgccgttga
     3241 tgttgtccgg cacattgatg ttgctgactg cattgttggt tactgcaaag gtgaaaatga
     3301 agaaatcctg atatcaggta aagaagcgtt ggtcagtagt tgttgctgtt cttacatatc
     3361 cgggcattct accttaccca acgcttccca ataattgctc tgattattac gttccatcgc
     3421 cacgatggcc tcgcgtatat gttgtttatt gttttcatct gacaacatta acttttccca
     3481 cgcttcatct gataatgacg catgtggcgt gcaaatttta cgtaaatctt gtaataccgt
     3541 ggttttctct tttgcggact gcagtgagct aaaaggcgac tcggcgtata cgatgcctgg
     3601 catagcgaaa aaagtgaaaa taagcagtag tgtcgttggt ttcatggatt caacctttta
     3661 caaatgtgta tcgaatgaag cataagtgta gtttgctttc atcgctctca tcatgctgta
     3721 cgaaacgcgc ttgcgtggtc agtaagaagt gccagacttt atattccact tttattcctt
     3781 tttattctta tcgatagcgt ttcgtttttt aaaccgcagc gaccttaccg c
//
LOCUS       DAZPAB010000273         3807 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZPAB010000273 DAZPAB010000000
VERSION     DAZPAB010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.274"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            437..2090
                     /gene="ushA"
                     /locus_tag="ACHUPG_003895"
                     /pseudo
     CDS             437..2090
                     /gene="ushA"
                     /locus_tag="ACHUPG_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar hydrolase/5'-nucleotidase
                     UshA"
     gene            complement(2101..2271)
                     /locus_tag="ACHUPG_003896"
                     /pseudo
     CDS             complement(2101..2271)
                     /locus_tag="ACHUPG_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2404..2523)
                     /locus_tag="ACHUPG_003897"
                     /pseudo
     CDS             complement(2404..2523)
                     /locus_tag="ACHUPG_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2597..3596)
                     /locus_tag="ACHUPG_003898"
                     /pseudo
     CDS             complement(2597..3596)
                     /locus_tag="ACHUPG_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1053 a          878 c          874 g         1002 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tcataattgg ttatgatgca atggccgtcg
      781 gtaatcatga atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat
      841 ttcctttcct ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat
      901 ggaaaatttt catccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata
      961 ctgcgaaaac aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg
     1021 aggcgaggag tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg
     1081 caacacatat gggtcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg
     1141 aaatggctcg cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag
     1201 ctcctgtctg catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct
     1261 gtgtaccaga taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg
     1321 tcggtcaggc tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac
     1381 acccagttaa tctcaaaatg agaattacca gagaagatgg aaaaacggaa ttttcttttt
     1441 acacccccga aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata
     1501 aaggcaaagc acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca
     1561 gaagtaaagt tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag
     1621 aacgtattga cgcagatttt gctgttgtca gtggtgggga aattcgagat tccatagaga
     1681 gtggtaacat cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa
     1741 gtattgattt gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac
     1801 cggattccgg agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa
     1861 tcgaaatgct aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa
     1921 catttagctt taatgctact ggtggggatg gctatccacg tattgataat agacccggat
     1981 acataaatac cggatttatt gatgctgaag tacttatcga atatatccgg aaacattcac
     2041 cgctggatgc tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt
     2101 gacgatcatc acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg
     2161 tatgtttgcg atatgggaat gcagttgctg tattttgtgt ttctgcttct gccagttgtt
     2221 gctgaattta accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt
     2281 cgctgattaa agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccatat
     2341 tgatatttac taagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc
     2401 cggtcagtcc tggatatgct caatgacgta acgcagccat ttttctggct ccacattgtt
     2461 cagacggcat gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctatcgga
     2521 accccgccgg agagagtttc actctccggc tgctcacgga accgtacgtg acactctcgc
     2581 gtcatacggc tcttgatcac tcattcgatg cacgtagtaa ataccagtgg gcaaatagct
     2641 tcggatactg tttccttacc agcgccagct ttcgctgagc cttccggttc gaaagtctgt
     2701 atttagactg catccactta agcaaacgtg actgcattgc actccacagt cgatagctga
     2761 agtttctgga ccagaacttt ccatagtact caatccagcc tctcactatc gcattgtagc
     2821 gcctcgcaaa atccagcaaa ctctcagctg ttgagcgatg tatacgccac ttcttgattg
     2881 tttctgttat tttgcacatt gctgcatttg atgcacccgg catacatttt ctgtacaatt
     2941 cgcctttgaa attcttcaga gtacgcactt tgaagtcata tccgagagtg gcatacaaca
     3001 tcatcggcgt acctttcgaa gggaacgccg ggaaactgac gttgcatcca catatcaaag
     3061 gcatagtgca ggaacagatt tgagagcagg ggggagatta cgccgccctg cggcgttcct
     3121 cgaccccgtg ccagcattgc tccatccggc atttgcaccg gtgccttcag cctgcgctca
     3181 atgtacatca ccacccagcg tgcctgagta tgatgctgca ctgtcttcag tagcaggtca
     3241 tgatcgatat catcaacgaa gcctttaata tcgacttcca ccacccagtc aaacttccag
     3301 cagcgtttcc tcgcctgcgc aatcgcctgg tgtgcggatt tacccggtcg ataaccatac
     3361 gagtctgcat ggaacagggg ttccagtacc ggctcgagta tctgcttaac gaccatctgg
     3421 gcgatccggt ccgacacggc cggaataccg agcggacgta caccaccgtc agctttggga
     3481 atgtccacac gccttaccgc ctgcggcata taactgccgg aagccattct gttccacagt
     3541 ttgtagagat tgtctgtaac attctcgtca aacccggcca gtgtctgccc gtcaatgcct
     3601 gccgccccac ggttggcttt tactcgttta taagcttccc atacctgtcg tttacttaca
     3661 ggaaatggct ttgccgattt tacagcagtc ctcccatcgg cattgtcgcc cgtggttgcc
     3721 gccgcaccat cagcacaatg gggcaagccc tttgctccgg acccattacg gtccttcacc
     3781 gctactacgg tttactccgt ccctgta
//
LOCUS       DAZPAB010000274         3777 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZPAB010000274 DAZPAB010000000
VERSION     DAZPAB010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.275"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            185..631
                     /locus_tag="ACHUPG_003899"
     CDS             185..631
                     /locus_tag="ACHUPG_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR0863290.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            642..2093
                     /locus_tag="ACHUPG_003900"
     CDS             642..2093
                     /locus_tag="ACHUPG_003900"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFR0863291.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            2083..3153
                     /locus_tag="ACHUPG_003901"
     CDS             2083..3153
                     /locus_tag="ACHUPG_003901"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR0863292.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            3153..>3777
                     /locus_tag="ACHUPG_003902"
     CDS             3153..>3777
                     /locus_tag="ACHUPG_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFR0863293.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT          856 a          979 c         1015 g          927 t
ORIGIN      
        1 aaaaataatg aataacccat taatgattca tgtggtttat ttaaataacc attatgtgca
       61 ttactccgca aatctgacct ttcactctgt ctcaaattgt cattgtttac cgacatatcg
      121 cctctcatca cttaatcgct ccaccattac aattgattaa aatatatttt atggagtccg
      181 attaatggca gctcttactg caagctgtat tgacctgaat attcagggca atggcgctta
      241 ttccgttctg aagcagttgg cgacaatagc gttacaaaac ggttttatca ccgactcaca
      301 ccagttcctg caaaccctgc tgctgcgcga aaaaatgcac tctactggat ttggttccgg
      361 tgtcgcagtg ccgcacggta aaagcgcctg cgttaaacaa ccgttcgtat tattcgcccg
      421 caaagcgcag gctattgact ggaaagccag cgatggcgaa gacgtcaatt gctggatctg
      481 cctcggcgtg ccgcaaagcg gcgaagagga tcaggtcaaa atcatcggca cactgtgtcg
      541 caaaattatt caccaggaat ttattcatca actgcaacag ggcgataccg accaggtgct
      601 ggccttgtta aatcaaaccc tcagctcata aggaagtggc gatggagtca tccttacgta
      661 ttgtcgcgat caccaactgc cccgccggga tcgctcacac ctacatggtg gcggaagccc
      721 tggaacagaa agcccgttct ctcggccata ccataaaagt ggaaactcaa gggtccagtg
      781 gcgttgaaaa ccgcttatcc agcgaagaga ttgccgctgc cgattacgtc attcttgcta
      841 ccgggcgtgg cctgagcggt gatgatcgcg cgcggtttgc cgggaagaaa gtttatgaga
      901 ttgccatctc cctggcgttg aaaaatatcg accagatttt cagcgaatta ccgacaaact
      961 cgcagctttt tgccgcagat agcggcgtga agcttggcaa acaggaagtg caaagcggca
     1021 gcgtgatgag ccacctgatg gcgggcgtct ctgcggcgct gccgtttgtc atcggcggtg
     1081 gtatcctggt ggcgcttgcc aacatgctgg tgcagttcgg tttgccgtat accgatatgt
     1141 cgaaaggcgc gccgtcgttt acctgggtag ttgaatccat cggttacctt ggcttcacct
     1201 ttatgatccc catcatgggc gcttatattg cctcgtcgat tgccgataaa ccggctttcg
     1261 ctccggcgtt tctggtttgc tatctggcga acgacaaagc gctgctcggc acccagtcgg
     1321 gtgcgggatt cctcggcgcg gtggtgctgg ggctggcgat tggctatttc gtcttctggt
     1381 tccgcaaagt gcgccttggc aaagcattac aaccgctgct cggttcaatg ctgatcccgt
     1441 tcgttactct gctggttttc ggcgtgctca cttactacgt tatcgggccg gtgatgtccg
     1501 acctcatggg cgggctgctc cacttcctga ataccattcc tccatcaatg aagtttgcgg
     1561 cagcgtttct ggtcggtgcg atgctggcgt tcgatatggg cggccccatc aataaaaccg
     1621 cctggttctt ctgcttctca ctgctggaaa aacacattta tgactggtac gccatcgtcg
     1681 gcgttgtcgc gttgatgccg cccgttgcgg cgggtcttgc gacgttcatt gcgcccaaac
     1741 tgtttacccg acaggaaaaa gaggctgcca gtagtgccat tgtggtcggt gccacagtcg
     1801 caaccgaacc ggctatacct tacgctctgg cagctccgtt gccgatgatt accgccaata
     1861 cgctggcggg cggcattacc ggcgtgctgg tgatcgcttt cggaatcaaa cgtctggcac
     1921 ctggtttggg tatctttgac ccgctgatcg gcctgatgtc gccggtaggt tcgttttatc
     1981 tggtgctggc gattggcctc gcgctgaaca tttcattcat tatcgtcctg aaaggattgt
     2041 ggttacgccg caaagcgaaa gccgcgcaac aggagcttgt ccatgaacat tgagttactg
     2101 caacagttgt gcgaagccag cgccgttagc ggcgatgaac aggaagttcg cgacatcctg
     2161 ataaacacgc tggaaccttg cgttaatgaa atcacctttg atggtctggg cagctttgtt
     2221 gcccgtaagg gaaataaagg tccaaaagtt gccgttgtcg ggcatatgga tgaagtcggc
     2281 tttatggtca cccacatcga cgagagcggt tttctgcgtt tcaccaccat tggtggctgg
     2341 tggaatcagt cgatgctcaa ccatcgggta accatacgca cacacaaggg agtgaaaatc
     2401 cctggtgtga ttggtttcgt cgcgcctcat gcgttaacgg aaaagcaaag gcaacagccg
     2461 ctgtcatttg atgagatgtt cattgatatt ggcgcaaaca gtcgcgatga ggtggaaaaa
     2521 cgcggtgtgg cgatgggcga ttttattagc ccggaagcca attttgcctg ctggggcgaa
     2581 gataaagtgg tcggcaaggc gctggataac cgcatcggct gcgcaatgat ggctgaacta
     2641 ttgcagacgg tgaataatcc cgaaattacg ctgtatggcg tcggcagcgt ggaagaagaa
     2701 gttgggctac gcggggcaca aacctcggcg gaacacatta aaccggatgt agtgatcgtg
     2761 ctggataccg ctgtcgcagg cgatgttccg ggcattgata acattaaata cccgctgaaa
     2821 ctgggccagg ggccggggct gatgctgttt gacaagcgct acttccccaa ccagaaactg
     2881 gtagcagcgt taaaaaactg tgccgcacat aacgatttac cgctgcaatt ttccaccatg
     2941 aaaaccggag cgacggatgg cgggcgctac aacgtgatgg gcggcgggcg tccggttgtt
     3001 gcgctgtgcc tgccaacccg ctatctgcac gctaacagcg gcatgatttc aaaagccgat
     3061 tacgatgctc tgctcacgtt gatacggggt tttctgacga ccttaaccgc ggagaaagtc
     3121 aacgcgttta gccagttccg tcaggtggat taatgctaaa cgaacgccag ttaaagattg
     3181 tcgatctgct ggagcaacag ccgcgcacgc ctggtgagct ggcgcaacag actggcgttt
     3241 caggcaggac catcctgcgt gatattgact atctcaactt tacccttaac ggcaaagccc
     3301 gcattttcgc cagtggcagt gcgggctatc agctggaaat cttcgagcgc cgcagctttt
     3361 ttcagttgct gcaaaagcac gataacgacg atcggctgct ggcgctgtta ttactgaata
     3421 ctttcactcc ccgtgcgcaa ctcgcctcgg cgcttaattt gccagaaacg tgggtagcag
     3481 agcgtctgcc ccagttaaaa cagcgttatg aacgcacttg ttgcctggcc agccgccctg
     3541 gtttgggcca tttcattgat gagacagaag agaaactcgt tatcttgctg gcgaacttgc
     3601 tgcgcaaaga tccgttttta attccgctgg cgggcataac acgagacaac cttcagcatt
     3661 tatccacggc ctgcgacaac caacaccgct ggccgctcat acagggtgat tatctctcca
     3721 gcctgattct ggcgatttac gccctgcgta atcaactgac cgatgagtgg ccgcaat
//
LOCUS       DAZPAB010000275         3738 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZPAB010000275 DAZPAB010000000
VERSION     DAZPAB010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.276"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            108..2105
                     /locus_tag="ACHUPG_003903"
     CDS             108..2105
                     /locus_tag="ACHUPG_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFR0863294.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2168..3445
                     /locus_tag="ACHUPG_003904"
     CDS             2168..3445
                     /locus_tag="ACHUPG_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HFR0863295.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
BASE COUNT          904 a          852 c          941 g         1041 t
ORIGIN      
        1 ttccaccatg cattacgatt aacgccgcat agccagttga actttgctac tttgtgagag
       61 gtagtacctt ctatccagtg cgaatttaat taatggaata aatgattatg agtgaaaatg
      121 atacaatccc aaagaagtct acaagtcaga ttaacaaagc ggtattcttt acatctgctt
      181 tgctaatttt ccttcttgtc gcctttgccg ccgtattccc ggatgtcgcc gacaaaaatt
      241 ttaaactact tcagcaacaa atcttcacga atgccagctg gttctacatc cttgctgtgg
      301 ccctgatttt actgagtgtc acgttccttg gactctcacg ctacggtgat atcaagctgg
      361 gcccggacca tgcgcagcct gatttcagct accactcctg gtttgcgatg cttttttcgg
      421 cagggatggg gatcggcctg atgttctttg gcgttgccga acctgtaatg cattatcttt
      481 cgccacccgt tggcactcca gaaaccgttg cggcagctaa ggaagcaatg cgtctgacct
      541 ttttccactg gggactgcac gcatgggcaa tttatgccat tgtggcgctg attctggcgt
      601 tcttcagtta ccgtcacggt ctgcctttaa ctctgcgctc cgcactctat cccattattg
      661 gcgatcgcat atacggacct gtaggacatg cggttgatat tttcgctgtt ataggcacgg
      721 tctttggcgt tgcgacatca ctgggttacg gtgttttgca ggtgaatgcc ggtttgaacc
      781 atcttttcgg ggtgcccatc aatgaaacgg tgcaggtaat tctgatcgtg gtcatcacgg
      841 ggttagcgac gatttcagtg gtgtccggtc tggataaggg aatacgtatc ctgtctgaac
      901 tcaatctggg tctggctttg ttgctcctgg cgctggtcct gtgtctggga ccaaccgtgc
      961 ttctgctgaa gtcatttgtg gaaaatacgg gcggttatct ttcggaactg gtgagtaaaa
     1021 cgttcaacct ttacgcgtat gagcccaagt cgagcaactg gctggggggc tggacattac
     1081 tgtactgggg atggtggctt tcatggtcgc cgtttgtggg gatgttcatc gcacgggtct
     1141 cccgcgggcg aaccattcgc gagtttgtca ccggcgtgct gtttgttccc gcgggtttta
     1201 cgctaatgtg gatgacggtg tttggtaaca gcgcgatcta tctcattatg aaccaggggg
     1261 ccacagacct cgccaatact gttcagcagg atgtggcgct ggccctgttt aatttcctgg
     1321 agcatttccc gttctcttct gtgctgtcat tcattgcaat ggcgatggtc atcgtcttct
     1381 ttgtaacgtc tgctgattcg ggggcaatgg ttgtggatac tctggcatca ggtggagtgg
     1441 caaacacacc cgtctggcag cgaatattct gggcctcgct catgggcatt gttgcaattg
     1501 cgcttctcct tgccggaggg ctaagtgcgc tgcaaacggt gacaatagcg agtgcattgc
     1561 ccttctcagt gatcttacta atatctatat acggactttt aaaagctttg cgccgggatt
     1621 tgaccaagcg tgaaagcctg agcatggcga caattgctcc tacggctgca cgtaacccaa
     1681 ttccttggca gagaaggtta cgcaatatcg cgtatctgcc gaagcgatct cttgtgaaac
     1741 gttttatgga cgacgttatc cagcctgcca tgacgctggt tcaggaggaa ctgaacaagc
     1801 aggggacgat aagccacatt agtgatgcag tcgacgatcg tattcgtctt gaagtcgatt
     1861 tgggcaacga gctgaatttt atatatgaag tgaggcttcg cgggtatatc tcaccgacct
     1921 tcgcgctcgc cgcaatggat aatgatgagc agcagactga acaacatcga tattatcgcg
     1981 ctgaggttta tctcaaagaa ggcggtcaaa attatgatgt gatgggctgg aaccaggaac
     2041 agctgattaa tgacatactg gaccagtacg aaaaacacct gcacttcctg cacctggttc
     2101 gttaatagca acatgccgtc ctgggggcgg caattattat cccggccgca atatgaggga
     2161 atgcagaatg atttcacgct ggaaatggat gctgaagcag acaattaaaa aactatggtt
     2221 cagggcaacg ttattcgcaa ttgtcgcgat aataacggcc cttttatcaa ttctttttaa
     2281 atcaatgata cctgagtcgg tttccgtgaa ggttggtgcg gaagcagtcg ataacattct
     2341 gaacatactg gcatcgagta tgctggcagt gaccacattt tcgctgagta tcatggtcac
     2401 agcctacggt tcagccacta ctaatgtgac tcccagagct acgcgtttag ttgttgaaga
     2461 cgtcaccaca caaaatgtac tggccacctt catcggttct tttctcttca gtctggtagg
     2521 gattattgcc ctcaatatgg gagcttatgg agaaaggggg agagtcattt tattcattgt
     2581 cacactggtt gtcattgcct taatcctcat cacattgctt cgctggatac agcatttgac
     2641 ctctctgggg agggttggtg agacaacggc acaagtagaa caggcggcca tcgaaacatt
     2701 tattgcgaga gcaagaaatc cctgtctcgg tggatatcca tggcttgaga acaatgaaca
     2761 gccgaaagga acggttgcag tttatccgaa gaagattggc tatgttgaat atattgatat
     2821 ggtgaaactt agcaagctgc tgaccaatga tccccgtcat gtatacctcg tggcgcagcc
     2881 aggcagtttc atacatccgt ccatgccagt tttgtacctg agtcagggcc aggagtcgtc
     2941 aatcaacacc gatttacttg agacgattat tgtctcggat gcacgttcat ttgctcagga
     3001 tcctcgattt tgtcttagcg tcatggccga aatagcctgc cgggcccttt cccctgcagt
     3061 gaacgatcct ggaaccgcca ttgatgtcat tggcagaggt gttcgtatac tttccactta
     3121 cgcgcagaat aaatctgatg aaatagaagt gaaatatcct tcagtacatg ttgcaccact
     3181 tcagaataac gatctactgg aagacttttt ctcacctgtc gcgcgcgatg gtgccagtat
     3241 gagggagatt cagataagag tccttaaagg actgtcgatg ctgagtaaag gttggcctga
     3301 gatatttgct gaagccgcac ataccctggc atttgaaaca ttagagcatg caactcgtgc
     3361 tgaccatata gattctgata gatatctaat aaaatcaact tattataatt tatttagtgg
     3421 cgaatattct aataaaaaaa catagctgca gagcaagcaa ttcggttgtt ggcaagtcaa
     3481 gggcaaagca ttcgcgtgag aatgggcgta tgctttgtgc caaaagcaaa cgttgctaat
     3541 tctgcactgt tcagcgatgg cgcagtagag acaggcactg ccactgtcca gtcggcaaaa
     3601 gcgaacactt tgccactgcg gggataaact gattgaagct ctcgcgcaaa atcacttatt
     3661 cttttgcgtt aataaaatgt aggagatggg tcattgatcc tacccacgta atatggacac
     3721 aggcctaagc gaggttct
//
LOCUS       DAZPAB010000276         3728 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZPAB010000276 DAZPAB010000000
VERSION     DAZPAB010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.277"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..224
                     /locus_tag="ACHUPG_003905"
     CDS             <1..224
                     /locus_tag="ACHUPG_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR0863296.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..479
                     /gene="hha"
                     /locus_tag="ACHUPG_003906"
     CDS             270..479
                     /gene="hha"
                     /locus_tag="ACHUPG_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR0863297.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            518..1108
                     /locus_tag="ACHUPG_003907"
     CDS             518..1108
                     /locus_tag="ACHUPG_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR0863298.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1348..1608
                     /locus_tag="ACHUPG_003908"
     CDS             1348..1608
                     /locus_tag="ACHUPG_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR0863299.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1832..1906
                     /gene="tap"
                     /locus_tag="ACHUPG_003909"
     CDS             1832..1906
                     /gene="tap"
                     /locus_tag="ACHUPG_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR0863300.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1899..2756
                     /gene="repA"
                     /locus_tag="ACHUPG_003910"
     CDS             1899..2756
                     /gene="repA"
                     /locus_tag="ACHUPG_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR0863301.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3457..3648
                     /locus_tag="ACHUPG_003911"
     CDS             3457..3648
                     /locus_tag="ACHUPG_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863302.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT         1020 a          907 c          934 g          867 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcggaat
      421 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
      481 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
      541 cattgttctg attattgcag cgctacttat ccggattatc ctcagaagtg taaatcagca
      601 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg actggtgatg cagagcgaat
      661 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
      721 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
      781 acgggtagcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
      841 gcagttattc aggatggtgt ctcagtggca tgttgatgtg gttatcgttg agctgcgttc
      901 gttcagtatt gttgcagcag tagagctgga tgatgccagc catttacgac cggaacgcag
      961 acgccgggat attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag
     1021 ccacgatgcc agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctga
     1081 tcagcagtca ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg
     1141 aaacgggaaa aagcaagttt tcctcgctcc cggcgtttcc ataactgaaa accatactat
     1201 ttcacagttt aaatcacatt aaacgacagt aatccccgtt gatttgtgcg ccaacacaga
     1261 tcttcgccac aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga
     1321 tccctgtttg agtattgagg aggcgagatg tcgcagacag aaaatgcagt gacttcctca
     1381 ttgagtcaaa agcggtttgt gcgcagaggt aagcctatga ctgactctga gaaacaaatg
     1441 gccgctgttg caagaaaacg tcttacacac aaagagataa aagtttttgt caaaaatcct
     1501 ctgaaagatc tcatggttga gtactgcgag agagagggga taacacaggc tcagttcgtt
     1561 gagaaaatca tcaaagatga actgcagaga ctggatatac taaagtaaag actttacttt
     1621 gtggcgtagc atgctagatt actgatcgtt taaggaattt tatggctggc cacgccataa
     1681 ggtggcaggg aactggttct gatgtggatg tacaggagcc agaaaagcaa aaaccccgat
     1741 aatcttcttt aactttggcg agtcagaaag attaccgggg cccacttaaa ccgtatagcc
     1801 aacaatcaag ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
     1861 gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt caacaaactt
     1921 attaccgcca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaaggt gcccggacgc
     1981 ttccgttctg cgggaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
     2041 ccattcatgt ggcacatgcc cgttcgcttg gtctgcgtcg gcgcatgcca ccggtactac
     2101 gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt tcactatgac ccactggcca
     2161 accgcgtcca gtgttccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     2221 ctgccggaaa actctccatc acccgggcca cccgggccct gacgttcctt gcagagctgg
     2281 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgata
     2341 tcacgttcac atctgcactg tttgctgccc tcgatgtatc agaggaggca gtggccgccg
     2401 cgcgccgcag tcgtgtggaa tgggaaaaca gacagcgcaa aaagcagggg ctggataccc
     2461 tgggtatgga tgaactgatg gcgaaagcct ggcgttttgt tcgtgagcgt ttccgcagtt
     2521 accagacaga gcttaagtcc cgtggaatga agcgtgcccg tgcgcgtcgt gatgcggaca
     2581 ggcagcgtca ggatattgtc accctggtga aacggcaact gacgcgtgaa atctcggaag
     2641 ggcgcttcac tgccagtcgt gaggcggtaa aacgtgaagt ggagcgtcgt gtgaaggagc
     2701 gcatgattct gtcacgtaac cgtaattaca gccggctggc cacagcttcc ccctgaaagt
     2761 gacctcctca gaataatccg gcacgcgccg gaggcattcg cacgcctgaa gcccgccagt
     2821 gcacaaaaaa tcagcgctac atacaaaaaa caacctcatc atccaccttc aggtgcatcc
     2881 ggtcccccct gtttttgata caaaacacgc ctcacagacg gggaattttg cttatccaca
     2941 ttaaactgca agggacttca ccataaggtt acaaccgttc acgtcataaa gcgccagacg
     3001 tcaaccctac agggtgcaat gtatctttta aacacctgtt tatatctcct ttaaactact
     3061 taattacatt catttaaaaa gaaaacctgt tcactgcctg tcctgtggac agacaaatat
     3121 gcacctccca ccgcaagcgg cgggccccca accgaagccg gtttagttac aacatgcaaa
     3181 cataaacccc taaaaaaccc aagtccaacg caaaactgaa accacaacgc ccccctcata
     3241 actgaaaagc gccgccgccc ccgcccaaag ggcgggaaca gagtcgcttt taattatgaa
     3301 tgttgtaact acatcatcat cgctgtcagt cttctggctg gacgtatcga atacacgctc
     3361 gtaagcggcc ctcacggccc gctaacgcgg agatacgccc cgacttcggg taaaaccctc
     3421 gtcgggacca ctccgaccgc gcacagaagc tctatcatgg ctgaaaacgg gtatggcttg
     3481 gcagggctgg ggatgggtaa ggtgaaatct gtcaatcagt accggctgac gccgggcttc
     3541 ggcggtttta ctccggtatc acatgtaacc acagcgtgcc gccttccatg ccgctggcgc
     3601 ggcatcagaa taatacaagc agcatttaat gcatttgcaa aagtgtgagt aatattaagt
     3661 ggtcgtttca gggatatcgt taagacttga gcgataggaa ttaaaacccc aaaaagatta
     3721 aaaaaaca
//
LOCUS       DAZPAB010000277         3703 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZPAB010000277 DAZPAB010000000
VERSION     DAZPAB010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.278"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            234..1646
                     /gene="ospC1"
                     /locus_tag="ACHUPG_003912"
     CDS             234..1646
                     /gene="ospC1"
                     /locus_tag="ACHUPG_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HFR0863303.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(1750..1899)
                     /locus_tag="ACHUPG_003913"
                     /pseudo
     CDS             complement(1750..1899)
                     /locus_tag="ACHUPG_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1975..3672
                     /gene="ospD3"
                     /locus_tag="ACHUPG_003914"
     CDS             1975..3672
                     /gene="ospD3"
                     /locus_tag="ACHUPG_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HFR0863304.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1334 a          610 c          651 g         1108 t
ORIGIN      
        1 tatagttatt aaattatgaa aataatttca caaaaaagat taacttgagt tcttaagcat
       61 cggattttag atattttgaa atatctggca tcacataaca tcaaaagtat ataatactct
      121 tatctaatta catgcagtat attgtatata tactcaaaaa ttgtaattca taccctgcta
      181 tatcttgtag ggaaagatat taattaaaac tgttttcata taaggttcat tttatgaata
      241 tatcagaaac actgaactca gcaaataccc aatgcaatat agattctatg gataacagat
      301 tacatacatt gtttccaaaa gtgacatcag tgcgaaacgc tgcacaacaa actatgccag
      361 atgaaaaaaa tttaaaagat agtgcaaata ttattaaaag tttctttagg aaaactatag
      421 cagcacagag ttatagtaga atgttctctc aaggctctaa ctttaaatct ttaaatatag
      481 caattgatgc accatcagac gctaaagcct catttaaggc tattgagcac cttgacagat
      541 tatcgaagca ttatatatct gaaataaggg aaaaacttca tcctctttct gcagaggaac
      601 tcaatttgct ttcgctaatt attaattctg atttaatctt cagacatcaa agtaattctg
      661 atttgtctga taaaatttta aacattaagt cattcaataa aattcagtct gaaggaatat
      721 gcacaaaacg aaacacatac gctgatgata taaaaaaaat agctaatcat gactttgtgt
      781 tttttggcgt tgaaatctct aaccatcaga aaaaacaccc cctgaataca aaacatcaca
      841 ctgttgattt tggtgcaaat gcgtatatca ttgatcatga ctctccatat ggatatatga
      901 cattaaccga tcactttgat aatgctattc cacctgtttt ttaccatgag caccaatcat
      961 ttttagataa attttcagag gttaataaag aagttagtcg atacgtacat ggaagtaaag
     1021 gaattataga tgtaccaata ttcaatacta aagatatgaa gttagggctc ggattatacc
     1081 tgattgactt tattagaaaa agtgaagacc aaagcttcaa ggagttttgc tatggaaaaa
     1141 atcttgcccc tgtggatctg gatagaatca taaactttgt ttttcagcca gagtaccata
     1201 tacctaggat ggtaagtaca gaaaacttca aaaaagttaa gattagagaa atatccttag
     1261 aggaggctgt tacagcatct aattacgaag aaattaacaa gcaggtcact aacaaaaaaa
     1321 ttgctctcca ggctcttttt ctttcgatta ctaatcaaaa agaggatgtc gccttatata
     1381 tattatctaa ttttgagata actagacaag atgttatttc cataaagcat gagttgtatg
     1441 atattgagta tctacttagc gctcataatt caagctgtaa agtacttgag tattttatca
     1501 ataagggatt ggttgatgta aacacaaagt tcaaaaaaac taatagtggg gattgtatgt
     1561 tggataacgc aataaaatat gagaatgcag aaatgataaa actattattg aaatatggtg
     1621 caacatctga caataaatat atttaatcaa aattgaatat cgtttagcaa aaggcagtat
     1681 ttgcacaaaa tatcgatata ttgtttattg tcagtatggc tcaatgtgat aatagttgga
     1741 aagtttgatg ggtttcgccc cgttgtagcg gtagtcgaac tggaaggtct tcaggcactg
     1801 cttacagcga tagagcagcc cccctgaact ggaatggccg ttccggatac ccccctgagt
     1861 ttcagagtaa cggtgacaaa ccacatcaat ctttgccatc aatcatccaa agggcaaaga
     1921 gtacaacaac actaagtctg cgtcacaacc catcaatgaa aggaatatat acatatgcca
     1981 tcagtaaatt taatcccatc aaggaaaata tgtttgcaaa atatgataaa taaagacaac
     2041 gtctctgttg agacaatcca gtctctattg cactcaaaac aattgccata tttttctgac
     2101 aagaggagtt ttttattaaa tctaaattgc caagttaccg atcactctgg aagacttatt
     2161 gtctgtcgac atttagcttc ctactggata gcacagttta acaaaagtag tggtcacgtg
     2221 gattatcatc actttgcttt tccggatgaa attaaaaatt atgtttcagt gagtgaagaa
     2281 gaaaaggcta ttaatgtgcc tgctattatt tattttgttg aaaacggttc atggggagat
     2341 attatttttt atattttcaa tgaaatgatt tttcattccg aaaaaagcag agcactagaa
     2401 ataagtacat caaatcacaa tatggcatta ggcttgaaga ttaaagaaac taaaaatggg
     2461 ggggattttg tcattcagct ttatgatccc aaccatacag caactcattt acgagcagag
     2521 tttaacaaat ttaacttagc taaaataaaa aaactgactg tagataattt tcttgatgaa
     2581 aaacatcaga aatgttatgg tcttatatcc gacggtatgt ctatatttgt ggacagacat
     2641 actccaacaa gcatgtcctc cataatcaga tggcctgata atttacttca ccccaaagtt
     2701 atttatcacg cgatgcgtat gggattgact gagctaatcc aaaaagtaac aagagtcgta
     2761 caactatctg acctttcaga caatacgtta gaattacttt tggcagccaa aaatgacgat
     2821 ggtttgtcag gattgctttt agctttacaa aatgggcatt cagatacaat cttagcatac
     2881 ggagaactct tggaaacttc tggacttaac cttgataaaa cggtagaact actaactgcg
     2941 gaaggaatgg gaggacgaat atcgggttta tcccaagcac ttcaaaatgg gcatgcagaa
     3001 actatcaaaa catacggaag gcttctcaag aagagagcaa taaatatcga atacaataag
     3061 ctgaaaaatt tgctgaccgc ttattattat gatgaagtac acagacagat acccggacta
     3121 atgtttgctc ttcaaaatgg acatgcagat gctatacgcg catacggtga gctcattctt
     3181 agcccccctc tcctcaactc agaggatatt gtaaatttgc tggcctcaag gagatatgac
     3241 aatgttcccg gacttctgtt agcattgaat aatggacagg ctgatgcaat cttagcttat
     3301 ggtgatatct tgaatgaggc aaaacttaac ttggataaaa aagcagagct gttagaagcg
     3361 aaagattcta atggtttatc tggattgttt gtagccttgc ataatggatg tgtagaaaca
     3421 attattgctt atgggaaaat acttcacact gcagacctta ctccacatca ggcatcaaaa
     3481 ttactggcag cagaaggccc aaatggggta tctggattaa ttatagcttt tcaaaatagg
     3541 aattttgagg caataaaaac ttatatggaa ataataaaaa atgaaaatat tacacctgaa
     3601 gaaatagcag aacacttgga caaaaaaaat ggaagtgatt ttctagaaat tatgaagaat
     3661 ataaaaagct gaatattatc tcgtggtgat acactgaatt tga
//
LOCUS       DAZPAB010000278         3643 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZPAB010000278 DAZPAB010000000
VERSION     DAZPAB010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.279"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            43..990
                     /gene="icsP"
                     /locus_tag="ACHUPG_003915"
     CDS             43..990
                     /gene="icsP"
                     /locus_tag="ACHUPG_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HFR0863305.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(1552..1924)
                     /locus_tag="ACHUPG_003916"
                     /pseudo
     CDS             complement(1552..1924)
                     /locus_tag="ACHUPG_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            2168..2287
                     /locus_tag="ACHUPG_003917"
                     /pseudo
     CDS             2168..2287
                     /locus_tag="ACHUPG_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            2537..3403
                     /gene="ospB"
                     /locus_tag="ACHUPG_003918"
     CDS             2537..3403
                     /gene="ospB"
                     /locus_tag="ACHUPG_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HFR0863306.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
BASE COUNT         1150 a          677 c          729 g         1087 t
ORIGIN      
        1 aagaagatca tggacatttc aaccaaaaaa gtagagttct cgatgaaatt aaaattcttt
       61 gtacttgcac tttgtgtacc tgcgatcttt actacacatg ctaccactaa ctatccactt
      121 ttcataccgg acaacatcag tacggatatt agtctaggat ctctgagtgg caaaacaaaa
      181 gaacgcgttt atcatcccaa ggaagggggg agaaaaatta gtcaactgga ctggaaatac
      241 agtaatgcga ctattgttag aggtggcatc gattggaagc taattccaaa agtgtctttc
      301 ggagtttccg gttggactac tttaggtaac cagaaagcaa gcatggttga taaagactgg
      361 aacaattcca atacccctca ggtatggacc gaccagagct ggcatcccaa tacgcatctc
      421 cgtgatgcta acgaattcga gctgaatctt aaaggttggt tattaaataa tttggattat
      481 cgacttggac taatagcagg ttaccaagag agtcgttaca gttttaatgc aatgggaggg
      541 agttatattt atagtgaaaa cggggggagc aggaataaaa aaggggcaca tcctagtggt
      601 gaaagaacaa taggttacaa acagctcttc aaaatacctt atattggatt aactgctaat
      661 taccgccatg agaattttga gtttggagca gaactgaaat atagtggttg ggttctttca
      721 tctgatacag ataaacacta tcagactgag acaattttta aagatgaaat aaaaaaccaa
      781 aattactgct ctgttgctgc gaatattgga tactatgtca cccccagtgc aaaattttat
      841 atagaaggct ccagaaatta catttctaat aaaaaaggtg atacatctct ttatgagcaa
      901 agtaccaata tatctggcac cattaaaaat agtgcaagta ttgaatatat tggttttctc
      961 acttccgcag gtataaagta tattttttga attggttcac cgagttgatt acgttttcat
     1021 ggctaaaaat tgccgcccat tgtgagtcat attcgccctg actttccaga accatacgaa
     1081 ctgcccgttg acggacttcg ggggaaaaac gtgtattttt agtcattctg tttacctctt
     1141 tctcagggcg tttagtctcc aggatttccg gggcggttca aaaaactctt cgttgattac
     1201 tgtggactta ctgttggcat taccgaccct gagaccggag aaataagaac cgcttagatc
     1261 atcgtggctg ttctcggggt atcaagttac acatgggcag aggctaccca aggtctacta
     1321 ttatcgcctg aaagcgctga tggaacagtg ctatcaggga catgctgatg aaaataccta
     1381 aataatcagc cagcactcta tctttccaaa tccacagcat agcaaagaga gcaaaagagc
     1441 ctgtaaattc agaaattaag gaaacgcctc ttggtgtaca taatctatct tatgttaaat
     1501 gaaatctaat ttcttgatta tatgctggat cgttcgtggg tggctttgcc cctatatttc
     1561 cagacacctg ttatcattgg cccgctgccg cagatattcc cgtggcgagc gataatccag
     1621 cgcactatgc ggatgccatt cgttataatg ctcgaatgcc tctgcaaggt tctttgctgc
     1681 cgttaacccg tcaggtttgg gcatgatact gatgtagtca cactttattg ttttcacgaa
     1741 gctctttgct attctgttac tctccggact ccgcaccgcc gtgttcttcg gttcaacaaa
     1801 gtcccaacat ccgggtgaac tggcgtgttt cattagctcg gtagcatgaa ccattatccg
     1861 tcagtcactt cactggagac gccggaagct cgttgccgaa gcggtgttcc accgctccca
     1921 gcatgtcgcc aacgggagag catctacggt ctgattgaac tgtttattca aattgtccac
     1981 cgtctgtcag tacgctctga acgtcggctg gttaaaacac tgctcgctga ttttcagagg
     2041 gttcacggta aaacagcact tttattccgg attgcggaag cggctctgaa taatccggat
     2101 gggctggtta aagaggttgt gtaccatctg catattgttg aaccagatcg cagcggtaag
     2161 agaagttcat catacttggc gcagttacgc gatgtttctg ctagaggtga tgccgtaaag
     2221 aacggcagaa cattaccgga acaagatagc ggtctacctg cactggtatc agacccgggg
     2281 cttccccgga tgatctccac ggtactttag gtactgtgtc tatattatgt aggtaggatc
     2341 aattaacttt gtagttgaaa ttctttcatt gaaataatga ataaaaaaga caaaccgcat
     2401 taaacaatgc cagaatataa ttgcattttt gttccgtttt tttaaatcat agtatacaaa
     2461 gaagcttgca tactatatga aaatataaca ccctttacta tgatgtttat aaaacaatat
     2521 atggagtcat gtaggtatga atttagatgg tgttagacca tactgtagaa tagtcaataa
     2581 aaagaatgaa agcatatcag atattgcatt tgcacatata ataaaaaggg taaaaaattc
     2641 atcatgtact cacccaaaag cagcattggt ttttttagga gagaaaggtt tttgtgatag
     2701 caatgatgtt ctatctatta tgggacaaca aataccaaga gtatttaaga acaagatgtt
     2761 atatgattat gtttttaaaa atgaaaaaag taaaaatgat tttctaaaaa tggctgaatc
     2821 atggctacca cagagtgaac caatagtaat aaataatgat gatgacgcat tgaatgctgc
     2881 tgcttatttt tctgtaaaaa aagcgaaaat aaaaacagta aacgatactg attttaaaga
     2941 gtataataag gtttatattc ttgggcacgg tagtcctggt tctcatcaat taggccttgg
     3001 ttcggaactt attgatgtac aaacaatcat ttcaagaatg aaagactgtg gtattctaaa
     3061 tgtgaaagat atccgtttta cttcatgcgg ctccgctgat aaagtggctc ctaaaaattt
     3121 taacaatgcc cctgctgaaa gtctttcttg tatccttaac tctctgcctt tttttaagga
     3181 aaaagaatct ttgctagagc agataaaaaa acaccttgaa aacgatgagt cattgagtga
     3241 tggtctaaaa atatccggct atcatggata tggagttcac tatggtcaag agctttttcc
     3301 ctactcacat tatcgttcaa cttcaattcc tgctgatccg gagcatacag taaaaagaag
     3361 ctctcagaaa aagactttta ttattaataa agaactggat tagtataaaa tttttaacct
     3421 atagttaatt gtttgcattt aagatgaagt tattttatct ttagtagaaa agtgcttccc
     3481 tatgagggaa aatcacaaat catcataata cagagacaaa acgatacgaa aaaatagata
     3541 aaaaacatca ttcttttacc acattatttt ccgtgaatat gaaaaataat gttattactt
     3601 taatataaga ctattttttg tttttccatc actctgttca aat
//
LOCUS       DAZPAB010000279         3572 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZPAB010000279 DAZPAB010000000
VERSION     DAZPAB010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.280"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            42..1193
                     /gene="yiaY"
                     /locus_tag="ACHUPG_003919"
     CDS             42..1193
                     /gene="yiaY"
                     /locus_tag="ACHUPG_003919"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFR0863307.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            1358..2896
                     /gene="aldB"
                     /locus_tag="ACHUPG_003920"
     CDS             1358..2896
                     /gene="aldB"
                     /locus_tag="ACHUPG_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFR0863308.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3115..3258)
                     /locus_tag="ACHUPG_003921"
     CDS             complement(3115..3258)
                     /locus_tag="ACHUPG_003921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863309.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            3441..>3572
                     /locus_tag="ACHUPG_003922"
     CDS             3441..>3572
                     /locus_tag="ACHUPG_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR0863310.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
BASE COUNT          888 a          855 c          939 g          890 t
ORIGIN      
        1 agtaagctgc ccaccctttt ttacactttc aggagtgtgt tatggcatct tcaactttct
       61 ttattccttc tgtgaatgtc atcggcgctg attcattgac tgatgcaatg aatatgatgg
      121 cagattatgg atttacccgt accttaattg tcactgacaa tatgttaacg aaattaggta
      181 tggcgggtga tgtgcaaaaa gcactggaag aacgcaatat ttttagcgtt atttatgatg
      241 gcacccaacc taaccccacc acggaaaacg tcgccgcagg tttgaaatta cttaaagaaa
      301 ataattgcga tagcgtgatc tccttaggcg gtggttctcc acacgactgc gcaaaaggta
      361 ttgcgctggt ggcagccaat ggcggcgata ttcgcgatta cgaaggcgtt gaccgctctg
      421 caaaaccgca gctgccgatg atcgccatca ataccacggc gggtacggcc tctgaaatga
      481 cccgtttctg catcatcact gacgaagcgc gtcataacaa aatggcgatt gttgataagc
      541 acgtgactcc gctgctttct gtcaatgact cctctctgat gattggtatg ccgaagtcac
      601 tgaccgccgc aacgggtatg gatgccttaa cgcacgctat cgaagcgtat gtttctattg
      661 ccgccacgcc gatcactgac gcttgtgcac tgaaagccgt gaccatgatt gccgaaaacc
      721 tgccgttagc cgttgaagat ggcagtaatg cgaaagcgcg tgaagcaatg gcttatgccc
      781 agttccttgc cggtatggcg ttcaataatg cttctctggg ttatgttcat gcgatggcgc
      841 accagctggg cggtttctac aacctgccac acggtgtatg taacgccgtt ctgctgccgc
      901 atgttcaggt attcaacagc aaagtcgccg ctgcacgtct gcgtgactgt gccgctgcaa
      961 tgggcgtgaa cgtgacaggt aaaaacgacg cggaaggtgc tgaagcctgc attaacgcca
     1021 tccgtgaact ggcgaagaaa gtggatatcc cggcaggcct acgcgacctg aacgtgaaag
     1081 aagaagattt cgcggttctg gcgactaatg ccctgaaaga tgcctgtggc tttactaacc
     1141 cgatccaggc aactcacgaa gaaattgtgg tgatttatcg cgccgcaatg taatcatcat
     1201 tccaacaacg gctggcaaat tgtcagccgc tttttcaact atctctgtaa tccttgcccg
     1261 taaattcgtg atagctgtcg taaagctgtt accgactggc gaagatttcg ccagtcacgt
     1321 ctacccttgt tatacctcac accgcaagga gacgatcatg accaataatc ccccttcagc
     1381 acagattaag cccggcgagt atggtttccc catcaagtta aaagcccgct acgacaactt
     1441 tattggcggt gaatgggtag cccctgccga tggtgagtat taccagaatc tgacgccggt
     1501 gaccgggcag ctgctgtgcg aagtggcgtc ttcgggcaaa cgagacatcg atctggcgct
     1561 ggatgctgcg cacaaagtga aagataaatg ggcgcacacc tcggtgcagg atcgcgcggc
     1621 gattctgttt aagattgccg atcgaatgga acaaaacctc gagctgttag cgacagctga
     1681 aacctgggat aacggcaaac ccattcgcga aaccagtgct gcggatgtac cgctggcgat
     1741 tgaccatttc cgctatttcg cctcgtgtat tcgggcgcag gaaggtggga tcagtgaagt
     1801 tgatagcgaa accgtggcct atcatttcca tgaaccgtta ggcgtggtgg ggcagattat
     1861 cccgtggaac ttcccgctgc tgatggcgag ctggaaaatg gctcccgcgc tggcggcggg
     1921 caactgtgtg gtgctgaaac ccgcacgtct taccccgctt tctgtactgc tgctaatgga
     1981 aattgtcggt gatttactgc cgccgggcgt ggtgaacgtg gtcaatggcg caggtgggga
     2041 aattggcgaa tatctggcga cctcgaaacg catcgccaaa gtggcgttta ccggctcaac
     2101 ggaagtgggc caacaaatta tgcaatacgc aacgcaaaac attattccgg tgacgctgga
     2161 gttgggcggt aagtcgccaa atatcttctt tgctgatgtg atggatgaag aagatgcctt
     2221 tttcgataaa gcgctggaag gctttgcact gtttgccttt aaccagggcg aagtttgcac
     2281 ctgtccgagt cgtgccttag tgcaggagtc aatctacgaa cgctttatgg aacgcgccat
     2341 ccgccgtgtc gaaagcattc gcagcggtaa cccgctcgac agcgtgacgc aaatgggcgc
     2401 gcaggtttct cacgggcaac tggaaaccat cctcaactac attgatatcg gtaaaaaaga
     2461 gggcgctgac gtgctcacag gcgggcggcg caagctgctg gaaggtgaac tgaaagacgg
     2521 ctactacctc gaaccgacga ttctgtttgg tcagaacaat atgcgggtgt tccaggagga
     2581 gatttttggc ccggtgctgg cggtgaccac cttcaaaacg atggaagaag cgctggagct
     2641 ggcgaacgat acgcaatatg gcctgggcgc gggcgtctgg agccgcaacg gtaatctggc
     2701 ctataagatg gggcgcggca tacaggctgg gcgcgtgtgg accaactgtt atcacgctta
     2761 cccggcacat gctgcgtttg gtggctacaa acaatcaggt atcggtcgcg aaacccacaa
     2821 gatgatgctg gagcattacc agcaaaccaa gtgcctgctg gtgagctact cggataaacc
     2881 gttggggctg ttctgatata agaagctggt cgcattgggt attcattgcc tgatgcgacg
     2941 cttacgcgtc ttatcatgcc tacgggaacc tgaccgtagg ccggataagg cgtacacgcc
     3001 gcatccggca taaacaaagc gcacgttgtt acaatttacg ttttaaacct acgtgcgcaa
     3061 taacccagac tacgccaaca gaagtgcgtt tctggggcgt gttttgtcag gagtttaatc
     3121 tcgcgtttcg atggtgagcg ggcgaaccag gccgtaaatc accattggtg atccgaggcc
     3181 aatcagcaat aaacggtaga taattgaagg tttaatacgc ttaatctgta ctgtggatgt
     3241 tttactggag ttattcatta gtttggccaa tagttccact atttcaatat gataaacctc
     3301 gtcctgttga aaaaccagac agcacatttc agaaagtata taaaaaacaa tatgtcgtta
     3361 tataaataag gaaattgcac tgcatcatgg catttcataa tatgcgttaa ttgttctttt
     3421 ttatattctt tgagggaaat atgttcctgg actattttgc actgggagtg cttatttttg
     3481 tatttctggt gatattctat gggattatta ttttacatga tattccctac ctgattgcta
     3541 aaaaacgtaa tcatcctcat gccgacgcca tt
//
LOCUS       DAZPAB010000280         3549 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZPAB010000280 DAZPAB010000000
VERSION     DAZPAB010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.281"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="ACHUPG_003923"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="ACHUPG_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR0863311.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="ACHUPG_003924"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="ACHUPG_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR0863312.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="ACHUPG_003925"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="ACHUPG_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR0863313.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIKNYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          772 c          820 g          951 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tttataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggcg
//
LOCUS       DAZPAB010000281         3530 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZPAB010000281 DAZPAB010000000
VERSION     DAZPAB010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.282"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            175..1326
                     /locus_tag="ACHUPG_003926"
                     /pseudo
     CDS             175..1326
                     /locus_tag="ACHUPG_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc16 family transposase"
     gene            complement(1356..1784)
                     /locus_tag="ACHUPG_003927"
                     /pseudo
     CDS             complement(1356..1784)
                     /locus_tag="ACHUPG_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(2299..3342)
                     /locus_tag="ACHUPG_003928"
     CDS             complement(2299..3342)
                     /locus_tag="ACHUPG_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0863314.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
BASE COUNT          944 a          813 c          841 g          932 t
ORIGIN      
        1 ataacattcc agttataata ttcaataatc aatgcgcgag gtgctactaa tggtattact
       61 gcgcttacag atgatatatc agaaagcgcc cgttttagtt ttaatggagg gtgatcttac
      121 ccaccaatag tggacacagg actaagtgag taaactctca accagaggtg actcatgaca
      181 aaaccagtat caatcagcaa gaagccccgt aaacaacata cgcctgaatt tcgtaacgaa
      241 gccctgaaac tcgctgaacg catcggtgtg gccgccgcag cccgtgaact cagcctgtat
      301 gaatctcagc tttatgcctg gcgcagtaaa cagcagcaac aaatgagttc gtcagagcgc
      361 gaaagcgaac tggccgctga aaatgtccgc cttaaacgac aactggcgga gcaggctgag
      421 gaactgtcca tcctccaaaa ggccgcgaca tacttcgcga agcgcctgaa atgaagtatg
      481 tctttatcga aaatcatcgg gcagagttca gcatcaaagc gatgtgtcgt gtacttcggg
      541 ttgcccgcag cggctggtat gtctggctca ggcgtcgtca ccagatgagc ctgcgccaac
      601 agtttcggct cacctgcgat gccgctgtcc ataaggcatt ctttgaggca aagcagcgat
      661 acggtgctcc ccgccttgct gacgaactgc cggagttcaa tattaaaacc attgccgcca
      721 gcctgcgtcg tcaggggctg cgggcgaaag ccagccggaa gttcagcccg gtcagctacc
      781 gtgcacatgg cctgcccgta ttggagaatc tgctggagca ggacttcagc gccagtggcc
      841 cgaaaccaga agtgggcggg tgacatcacg tacttgcgta ccgatgaggg ctggttgtat
      901 ctcgcagtag tcatcgacct gtggtcacgc gccgtcattg gctggtcgat gtcaccgcga
      961 atgacagcac aactggcctg tgatgcactg caaatggcgt tgtggcggag aagacgcccg
     1021 gaaagcgtca ttgttcatac ggaccgtggt ggtcaatact gttcagggga ttatcaggca
     1081 atgctgaagc gacacaacct gcgtggcagt atgagtgcga aaggctactg ttatgacaat
     1141 gcctgtgtgg aaagcttctt tcattcgctg aaggtggaat gtatccacgg ggaacgcttt
     1201 agcagccggg aaataatgcg ggcaacggtg tttaattata tcgagtgtga ttacaatcgc
     1261 tggcgtcgtc acagtgcctg tggcggtctc agcccggaac aatttgaaaa ccataatctc
     1321 gcttagggcc gtgtccacat tacgtgggta ggatcagtat gggtcgtctt gtatttgtca
     1381 tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag caggtctctg
     1441 ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa ggctggcgtc
     1501 actatcggtg taacaatatc aggcgtgcct gcggaacggt ccttaccatg ctgcagtaac
     1561 cggaaataaa cttttgggcg taagatgaca ttgacagttt cgacatcgtc tttttctccc
     1621 ttaaaaaatt tattgacaat cgcttcgtca agacgtcccg ccgctatccc atgccagtga
     1681 gtgaaatttt gggcgtcttt ccgttcaaaa cagccctttc ctgactcagc atctgcaagc
     1741 gagttgcgcc agtatgaggc aaaccttaaa gcattttcat ccattaccag tcctgtgact
     1801 caatgaaatc cattgattta tcgaatataa aaccaccgta tggacaacta ttgtccatat
     1861 taaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgtact ggttgtacga
     1921 ggttcagaac acaagcacat tcaacagtac gtcaggacta ttgatttcat aacagcgaat
     1981 acaatttttt actgtttata atggctgcct gtgttggacg gggataccag ttcaaccgaa
     2041 aacgccagag gaggggatta cccgctggca gggtaaatct gtggtttttg atgccagcta
     2101 taacgtgttt gacgataaag tattacgaaa gaactttaat gagttcatgg agtaattcgt
     2161 taaagtaatt tacaacgcta tccttttgca aaggaaactt tcagaaaata accccagggt
     2221 aatcacctgg ggaaggaaca cagtgttatt attggataat aaattattac ttgttttaaa
     2281 gttacactaa tgcaatatct actgatactg gatgcttgaa tgtttcatcc ctgctgcgga
     2341 aaagggcaat atccagggcc agatatattc accgttcata tttctgcatt tctgtacaat
     2401 ttcctcagaa agtgtcttgt caattctttt ccacaggtgc tcctgctgtc ttaaaatact
     2461 ttcgcatatt tcgaaatcat cttcctgaat cagcatttcg gcgcatttaa tgatttctga
     2521 tcgttcatgc agcacggagg aatggttata gagccagttt acaaacagca atgcttccgg
     2581 atattgtccc cataatggtg agtatgttgc atagtaatct tctgccgtat ccagagagcc
     2641 ggagaagtca tgccacacag caagtggaaa tggatgcaga tttccgcaca gcatttctgc
     2701 gatgtaagtg tttgtggcaa agccatggcg aaaagctgtg gcggccttaa catgttcccc
     2761 gttcagaaaa tgcaggagac cgagttcata ccagtaaggt gaaaattcat cagcatgctc
     2821 tttcagtaca ctgaaggctc gctcatgatc gccggtgcgt aacagctctg aacccaacaa
     2881 ccagcgagcc ccctgattat cttcaggatt gtatgccagt attttatcag tgagcataac
     2941 cgcatcctga tggcgctgta agtgcacatt tgcgagaatt gctgcataaa gcgcgcgcag
     3001 ataaggccgg ttttcgggat gcatccagat tatttcacca cagaaacttt ccgggatgat
     3061 acgattacct gccgccagtc ctttaagagc ggcgttcagt gccttacgcg gggcattctg
     3121 ttccagaaat gcatatgcca gatgagcgtg tatatcaata aagtccggct cccgttgagc
     3181 aaggcgtcgg agtttgttga tgtaactcgt ctcgctgatt tcatcagcgt cgtatgcatc
     3241 aagcacttct tcaaactccc ccagcaatgg gccaaaccag gtatccggaa atacgaaata
     3301 acccttgtta tcgtgcactt caaagcataa tctatcgttc attttctgag cctgtaaaac
     3361 atgtggtgat atagtctgag agcataaaag ttgagattct gtgtatcaag tcctgtcttg
     3421 gcaccgaatg aatattgctt acgaagcccc ttcatgcttt ccttgagaac aataatttta
     3481 tctttatgtg aatctcattt tttataatga tgtcagatga ttgaaatgct
//
LOCUS       DAZPAB010000282         3493 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZPAB010000282 DAZPAB010000000
VERSION     DAZPAB010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.283"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..311
                     /locus_tag="ACHUPG_003929"
     CDS             <1..311
                     /locus_tag="ACHUPG_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFR0863315.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="ACHUPG_003930"
     CDS             468..1217
                     /locus_tag="ACHUPG_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR0863316.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="ACHUPG_003931"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="ACHUPG_003931"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFR0863317.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="ACHUPG_003932"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="ACHUPG_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR0863318.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..>3493
                     /locus_tag="ACHUPG_003933"
     CDS             3361..>3493
                     /locus_tag="ACHUPG_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="YieH; catalyzes the dephosphorylation of
                     phosphoenolpyruvate, AMP and p-nitrophenyl phosphate;
                     purine and pyrimidine nucleotides are secondary
                     substrates; member of the haloacid dehalogenase-like
                     hydrolases superfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR0863319.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     "
BASE COUNT          961 a          908 c          863 g          761 t
ORIGIN      
        1 cactggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agtctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcaccagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aac
//
LOCUS       DAZPAB010000283         3488 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZPAB010000283 DAZPAB010000000
VERSION     DAZPAB010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.284"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..389
                     /locus_tag="ACHUPG_003934"
     CDS             <1..389
                     /locus_tag="ACHUPG_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0863320.1"
                     /translation="HGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITEN
                     GSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGW
                     LTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(367..990)
                     /gene="tetR(B)"
                     /locus_tag="ACHUPG_003935"
     CDS             complement(367..990)
                     /gene="tetR(B)"
                     /locus_tag="ACHUPG_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFR0863321.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1072..2277
                     /gene="tet(B)"
                     /locus_tag="ACHUPG_003936"
     CDS             1072..2277
                     /gene="tet(B)"
                     /locus_tag="ACHUPG_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFR0863322.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2390..2983)
                     /gene="tetC"
                     /locus_tag="ACHUPG_003937"
     CDS             complement(2390..2983)
                     /gene="tetC"
                     /locus_tag="ACHUPG_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HFR0863323.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3071..3457
                     /locus_tag="ACHUPG_003938"
                     /pseudo
     CDS             3071..3457
                     /locus_tag="ACHUPG_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014656611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          948 a          641 c          716 g         1183 t
ORIGIN      
        1 accatggcgt acatgccaaa gtttccacgc cagtgcattt acgaaaagca cgtacttgct
       61 atgatcattt agctggcgaa gttgccgtta agatctatga ttccctttgt caacagcaat
      121 ggatcactga aaatggttca atgatcacat taagtggtat tcaatatttt catgaaatgg
      181 gaattgacgt tccttccaaa cattcacgta aaatctgttg tgcgtgttta gattggagtg
      241 aacgccgttt ccatttaggt gggtacgttg gagccgcatt attttcgctt tatgaatcta
      301 aagggtggtt aactcgacat cttggttacc gtgaagttac catcacggaa aaaggttatg
      361 ctgcttttaa gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
      421 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
      481 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
      541 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
      601 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
      661 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
      721 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
      781 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
      841 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
      901 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
      961 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
     1021 tcattgatag agttatttta ccactcccta tcagtgatag agaaaagtga aatgaatagt
     1081 tcgacaaaga tcgcattggt aattacgtta ctcgatgcca tggggattgg ccttatcatg
     1141 ccagtcttgc caacgttatt acgtgaattt attgcttcgg aagatatcgc taaccacttt
     1201 ggcgtattgc ttgcacttta tgcgttaatg caggttatct ttgctccttg gcttggaaaa
     1261 atgtctgacc gatttggtcg gcgcccagtg ctgttgttgt cattaatagg cgcatcgctg
     1321 gattacttat tgctggcttt ttcaagtgcg ctttggatgc tgtatttagg ccgtttgctt
     1381 tcagggatca caggagctac tggggctgtc gcggcatcgg tcattgccga taccacctca
     1441 gcttctcaac gcgtgaagtg gttcggttgg ttaggggcaa gttttgggct tggtttaata
     1501 gcggggccta ttattggtgg ttttgcagga gagatttcac cgcatagtcc cttttttatc
     1561 gctgcgttgc taaatattgt cgctttcctt gtggttatgt tttggttccg tgaaaccaaa
     1621 aatacacgtg ataatacaga taccgaagta ggggttgaga cgcaatcgaa ttcggtatac
     1681 atcactttat ttaaaacgat gcccattttg ttgattattt atttttcagc gcaattgata
     1741 ggccaaattc ccgcaacggt gtgggtgcta tttaccgaaa atcgttttgg atggaatagc
     1801 atgatggttg gcttttcatt agcgggtctt ggtcttttac actcagtatt ccaagccttt
     1861 gtggcaggaa gaatagccac taaatggggc gaaaaaacgg cagtactgct cggatttatt
     1921 gcagatagta gtgcatttgc ctttttagcg tttatatctg aaggttggtt agttttccct
     1981 gttttaattt tattggctgg tggtgggatc gctttacctg cattacaggg agtgatgtct
     2041 atccaaacaa agagtcatca gcaaggtgct ttacagggat tattggtgag ccttaccaat
     2101 gcaaccggtg ttattggccc attactgttt gctgttattt ataatcattc actaccaatt
     2161 tgggatggct ggatttggat tattggttta gcgttttact gtattattat cctgctatcg
     2221 atgaccttca tgttaacccc tcaagctcag gggagtaaac aggagacaag tgcttagtta
     2281 tttcgtcacc aaatgatgtt attccgcgaa atataatgac cctcttgata acccaagagg
     2341 gcatttttta cgataaagaa gatttagctt caaataaaac ctatctattt tatttatctt
     2401 tcaagctcaa taaaaagccg cggtaaatag caataaattg gcctttttta tcggcaagct
     2461 cttttaggtt tttcgcatgt attgcgatat gcataaacca gccattgagt aagtttttaa
     2521 gcacatcatc atcataagct ttaagttggt tctcttggat caatttgctg acaatggcgt
     2581 ttaccttacc agtaatgtat tcaaggctaa ttttttcaag ttcattccaa ccaatgatag
     2641 gcatcacttc ttggataggg ataaggtttt tattattatc aataatataa tcaagataat
     2701 gttcaaatat actttctaag gcagaccaac catttgttaa atcagttttt gttgtgatgt
     2761 aggcatcaat cataattaat tgctgcttat aacaggcact gagtaattgt tttttatttt
     2821 taaagtgatg ataaaaggca cctttggtca ccaacgcttt tcccgagatc tcatctattg
     2881 aaacagcttg atagcctttt tcaacaaaca atattcgtgc tgagttaacc agtgattgat
     2941 aggtactctt aaaattttct tgttgatgat ttttattttc catgatagat ttaaaataac
     3001 ataccgtcag tatgtttatg gtatcatgat gatgtggtcg tgacaatctt aagaacattt
     3061 aggttatttt atgtatattg aacagcattc tcgctatcaa aataaagcta ataacatcca
     3121 attaagatat gatgataagc agtttcatac aacggttatc aaagatgttc tattatggat
     3181 tgaacataat ttagatcagt ctttactgct tgatgatgtg gcgaataaag cgggttatac
     3241 caagtggtat tttcagcggc tgttcaaaaa agtaacaggg gtcacactgg ctagctatat
     3301 tcgtgctcgt cgtttgacga aagcggctgt tgagttgagg ttgacgaaaa aaactatcct
     3361 tgagatcgca ttaaaatatc aatttgattc ccaacaatct tttacacgtc gatttaagta
     3421 catttttaag gttacaccaa gttattatcg gcgtaataaa ttatgggaat tggaggcaat
     3481 gcactgag
//
LOCUS       DAZPAB010000284         3456 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZPAB010000284 DAZPAB010000000
VERSION     DAZPAB010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.285"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(226..429)
                     /locus_tag="ACHUPG_003939"
     CDS             complement(226..429)
                     /locus_tag="ACHUPG_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863324.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="ACHUPG_003940"
     CDS             complement(431..586)
                     /locus_tag="ACHUPG_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR0863325.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="ACHUPG_003941"
     CDS             complement(789..1196)
                     /locus_tag="ACHUPG_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0863326.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="ACHUPG_003942"
     CDS             1273..1500
                     /locus_tag="ACHUPG_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR0863327.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="ACHUPG_003943"
     CDS             1484..1906
                     /locus_tag="ACHUPG_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR0863328.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1984..2761
                     /locus_tag="ACHUPG_003944"
                     /pseudo
     CDS             1984..2761
                     /locus_tag="ACHUPG_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2768..3456
                     /locus_tag="ACHUPG_003945"
                     /pseudo
     CDS             2768..3456
                     /locus_tag="ACHUPG_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          885 a          764 c          940 g          867 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagatttttg tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gctggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccctggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt
     1921 gccggattgt tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa
     1981 caaatggctt caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc
     2041 aggcttgctg agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc
     2101 tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt
     2161 acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag
     2221 gttggatggc tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac
     2281 ggaaaaagct ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg
     2341 aaacgaaaag gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa
     2401 gaggaagagg aagatctaaa tactgatctc cccctaaatc cccctcgcca aaaacgagcg
     2461 tctaaaaaat tcgagccgga ggctattgag ctgcccgatt ggttgccgga aacactctgg
     2521 catgagtggg tccggttcag acaggcattg cgaaaaccga ttcgaacgga gcagggcgat
     2581 acgggaactg gaaaaattcc gtcagcaggg ttttacacct gagcaggtga ttcgacacag
     2641 catcgccaat gaataccagg gcctgttcgc gccgaaaggt gttcggcctg agacgttgct
     2701 ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc ccgccaggct tcagggggta
     2761 acggaccatg aaaaatattg cgacaggcgg cgttctggaa cgcatccgcc gactgacccc
     2821 gcaacatgta accgccccat tcagaacggt agcggagtgg cgcgagtggc aacttgctga
     2881 aggccagaaa cgtagcgagg agatcaaccg cctgaatcgc cagttgcggg tggaaaaaat
     2941 tctgaatcgc tcaggcatcc agccgttgca ccgtaaatgc tcgtttgcga attaccaggt
     3001 gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa tccatcgccg atgaactgat
     3061 gaccgggtgt acaaattttg cgttcagcgg aaaacctgat accgggtaga atcacttagc
     3121 ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca gtgattgtgg ttaccgtggc
     3181 tgatgttatg agcgccctgc acgccagcta tgacgacggg cagtcaggcg aaaaattttt
     3241 gcgggagctg tgcgaagtgg atctgctggt tcttgatgaa attggcattc agcgcgagac
     3301 gaaaaacgag caggtggtgc tgcaccagat tgttgatcgc cggacagcgt cgatgcgcag
     3361 cgtggggatg ctgacaaacc tgaactatga ggtgatgaaa acattgctcg gcgagcgggt
     3421 gatggatcgc atggtcatga acggcgggcg ctgggt
//
LOCUS       DAZPAB010000285         3452 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZPAB010000285 DAZPAB010000000
VERSION     DAZPAB010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.286"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..77
                     /locus_tag="ACHUPG_003946"
     CDS             <1..77
                     /locus_tag="ACHUPG_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR0863329.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            435..1493
                     /gene="rfaQ"
                     /locus_tag="ACHUPG_003947"
     CDS             435..1493
                     /gene="rfaQ"
                     /locus_tag="ACHUPG_003947"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR0863330.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1490..2614
                     /locus_tag="ACHUPG_003948"
     CDS             1490..2614
                     /locus_tag="ACHUPG_003948"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR0863331.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2607..3404
                     /gene="rfaP"
                     /locus_tag="ACHUPG_003949"
     CDS             2607..3404
                     /gene="rfaP"
                     /locus_tag="ACHUPG_003949"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR0863332.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT          908 a          753 c          845 g          946 t
ORIGIN      
        1 cactgtcgtt ctcaaaatcg gtggagctgc atgacaaggt catcgggcat tatctgaaca
       61 taaaacacta tcagtaagtt ggagtcatta cccgtttttg acttaaatag caatttaccc
      121 gccaattctt gttgaacatc tatacttttt ttgtattttt atgcgattca ttgatttctg
      181 ctgtacgtgc aggaaggaat cgcagagcaa cagtgaaaga atatggttgc taatgatttt
      241 atttgattga tttctttatt gaaaatcaac aatgtgctta aaaatcattc gggtcggatt
      301 gccgcgaaag aagtgataca ctagcgcgtc aatatcagtg cggtatcagt attcaggtag
      361 ctgttgagcc tggggcggta gcgtgctttt tctgcttcac cttaatcgga taatctcaac
      421 aaaaagagtt acttgtggat aagccatttc gaagaatttt gctcattaag atgcgttttc
      481 atggggatat gttattaact actcccgtca ttagttctct gaaaaaaaat taccctgacg
      541 caaaaatcga tgtgctgctt tatcaggaca ccatcccgat cctgtctgaa aatccagaga
      601 ttaacgcgct ctacggcata aaaaataaaa aagcaaaagc ctcagaaaaa attgccaact
      661 tttttcatct catcaaggta ttacgtgcca ataagtatga ccttatcgtc aatcttaccg
      721 atcaatggat ggttgctata ctggttcgct tattaaatgc tcgtgtgaaa atttcccagg
      781 attatcatca tcggcagtct gctttttggc gtaaaagttt cacccatttg gtgccgttgc
      841 agggtggaaa tgtggtggaa agtaacttat ccgtgctgac accattggga cttgattcgt
      901 tggtgaagca gacaaccatg agttacccgc ctgcaagctg gaaacgtatg cgtcgcgaac
      961 ttgatcacgc tggtgttgga caaaattatg tggttatcca acctacggcg cggcaaatct
     1021 tcaaatgctg ggacaacgcc aagttttccg ctgtgattga tgccttacat gctcgtggtt
     1081 atgaagtcgt tctgacgtcc ggcccagata aagacgatct ggcctgcgtc aatgaaattg
     1141 cgcagggatg ccagacgcca ccagtaacgg cgctggctgg aaaggtgacc ttcccggaac
     1201 ttggtgcgtt aatcgatcat gcgcagctgt ttattggcgt tgattccgca ccggcgcata
     1261 ttgccgctgc agttaatacg ccgctgatat cgctgtttgg cgcaacagat catattttct
     1321 ggcgtccctg gtcaaataac atgattcaat tctgggcggg agattaccgg gaaatgccaa
     1381 cgcgcgatca gcgtgaccga aatgagatgt atctttcggt tattccggcg gcagatgtca
     1441 ttgctgctgt cgataaatta ctgccctcct ccacgacagg tacgtcgtta tgatcgttgc
     1501 tttttgttta tataaatatt ttccctttgg cggtttgcag cgcgatttta tgcgtattgc
     1561 tcagacagtc gccgcccgag gtcatcatgt tcgggtttat acccagtcgt gggaaggcga
     1621 atgccctgat gtatttgaag tgatcaaagt gccggttaaa tcgcatacca atcacgggcg
     1681 caatgcggag tattttgcct gggtgcaaaa acatttacgc gaacatcccg tcgataaagt
     1741 cgttggattc aacaaaatgc cggggctgga cgtttattat gccgctgatg tttgttatgc
     1801 cgagaaagta gcgcaggaaa aaggcttttt ctatcgcctg acgtcacgtt atcgccatta
     1861 tgccgccttt gagcgggcaa ccttcgaaca gggcaagccg acacagctgc tgatgctgac
     1921 agataagcaa atcgccgatt tccagaaaca ttatcagact gaagcggagc gttttcatat
     1981 tctgccaccg gggatttatc ctgatcgtaa atatagccag cagccagcca atagccgtga
     2041 aatcttccgt aagaagaatg gaataaccga acaacaatat ttattgttgc aggtcggttc
     2101 agacttcacg cgtaaaggtg ccgatcgttc cattgaagca cttgcttcgt taccggattc
     2161 gctgcgccac aacacattgc tatatgttgt tgggcaggat aaaccgcgaa aatttgaggc
     2221 actggcagaa aaacgcggcg tgcgcagtaa tgttcacttc ttctcggggc gcaacgatgt
     2281 ctcggaatta atggcggcgg cggatttatt actgcatcct gcctaccagg aagcggcggg
     2341 aattgtgctg ctggaagcga ttactgcagg attaccggta ctaacaactg ccgtttgtgg
     2401 ctatgcgcat tatattgtcg acgctaattg cggcgaggct attgctgagc cattccgcca
     2461 ggaaacattg aatgagattt tacgcaaagc gttaacgcaa tcttcattgc gccaggcttg
     2521 ggcggaaaat gcgcgacatt atgctgatac acaagattta tacagtctgc cagagaaagc
     2581 ggcggatatc ataacgggtg gtctggatgg ttgaacttaa agagccgttt gccacgttat
     2641 ggcgcggcaa agatcctttt gaggaagtta aaaccttgca gggtgaggta tttcgtgaac
     2701 tggaaactcg ccgtactctg cgctttgaaa tggcgggaaa aagctatttt ctcaaatggc
     2761 atcgcggcac gaccctgaaa gagataatca aaaatttact ctcattgcgg atgccagtat
     2821 taggcgcaga ccgcgaatgg aatgcgattc atcgactgcg ggatgtcggc gttgatacta
     2881 tgtatggggt ggcattcggc gaaaaaggca tgaatccgct gaccagaacc tcgtttatta
     2941 ttaccgaaga tctgacacca accatcagtc tggaagatta ctgtgctgac tgggcgacca
     3001 acccaccaga tgttcgcgta aagcgtatgc ttattaagcg tgtcgcgacg atggtgcgcg
     3061 atatgcatgc tgcgggcatt aaccatcgtg actgttatat ctgtcatttc ctgctgcact
     3121 tgcctttttc cggtaaggaa gaggagttaa aaatttcggt aattgaccta caccgggcgc
     3181 agcttcgcac gcgcgttcca ggtcgttggc gcgataaaga tcttataggg ctttattttt
     3241 cttcaatgaa tattggcctg actcagcggg atatctggcg ctttatgaag gtgtattttg
     3301 ccgccccgct taaagacatt ctcaagcagg aacaaggact gctgtcgcaa gcagaagcaa
     3361 aagccacaaa aatcagggaa agaacgattc gaaaatcgtt gtaacccgta acttattttt
     3421 gccaaaattt tggatacaga ataaatatgt ct
//
LOCUS       DAZPAB010000286         3426 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZPAB010000286 DAZPAB010000000
VERSION     DAZPAB010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.287"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            88..804
                     /gene="nanC"
                     /locus_tag="ACHUPG_003950"
     CDS             88..804
                     /gene="nanC"
                     /locus_tag="ACHUPG_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR0863333.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            824..1930
                     /gene="nanM"
                     /locus_tag="ACHUPG_003951"
     CDS             824..1930
                     /gene="nanM"
                     /locus_tag="ACHUPG_003951"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR0863334.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1995..2975
                     /locus_tag="ACHUPG_003952"
     CDS             1995..2975
                     /locus_tag="ACHUPG_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR0863335.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3085..3290
                     /locus_tag="ACHUPG_003953"
                     /pseudo
     CDS             3085..3290
                     /locus_tag="ACHUPG_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT         1002 a          728 c          803 g          893 t
ORIGIN      
        1 ctggcaaaaa aatattacac aacataaatg ctaattgttt atgcgggctt tgtattgctt
       61 tctgtatcct acaaatgagt gaaatttatg aaaaaggcta aaatactttc tggcgtatta
      121 ttactgtgct tttcgtcccc attaatttct caggctgcga cactggacgt acgtggtgga
      181 tatcgtagtg gaagccacgc ctatgagact cgactcaaag tcagtgaggg atggcaaaat
      241 ggatggtggg caagcatgga aagtaatacc tggaatacca ttcatgataa taaaaaggaa
      301 aatgccgcac tcaatgatgt tcaggttgaa gttaattacg caattaaact tgatgatcaa
      361 tggacggtgc gcccgggaat gttaacgcat tttagcagca acggcacacg ctacggaccc
      421 tacgtaaaac tgtcctggga cgcgacaaaa gatcttaatt ttggcattcg ctatcgttac
      481 gactggaaag cttaccgaca acaagactta tccggtgata tgtctcgtga taacgttcat
      541 cgttgggatg gatatgtcac ttaccatatt aatagtgatt tcaccttcgc atggcaaacg
      601 acgctataca gcaaacagaa tgattatcgc tatgcaaacc acaagaaatg ggcgacggaa
      661 aatgcctttg ttctacaata ccatatgacg cccgatatta cgccatacat agaatatgac
      721 taccttgacc gccagggtgt ttacaacggc agagataatt tatcggaaaa cagttatcgc
      781 attggtgtgt catttaaact gtagtagaca ggagacagtc acaatgaata aaactataat
      841 ggcgcttgct atcatgatgg cttcatttgc cgcaaacgcg tctgtattac cggaaactcc
      901 tgtgccattt aaaagtggta ccggagcaat tgataacgac actgtctaca ttggtttagg
      961 tagcgcaggt acagcatggt acaagctaga tacacaggcc aaagataaaa aatggactgc
     1021 gttagctgca ttccctggtg gaccgagaga gcaagcaacc tctgcattta ttgatggcaa
     1081 tctgtatgtg tttggcggca ttggcaaaaa cagcgaggga ttgactcagg tatttaatga
     1141 cgtacacaaa tacaacccca aaaccaatag ctgggttaaa ttgatgtcgc acgcgccgat
     1201 gggcatggcg ggtcatgtga cttttgtaca caacggcaag gcttatgtta ctggcggtgt
     1261 taaccagaat atcttcaatg gctattttga agatctcaac gaggctggaa aagattcaac
     1321 cgctatagat aaaatcaacg cccactattt tgacaaaaaa gcagaagatt atttcttcaa
     1381 taagtttctg ttgtcttttg atccctcaac acagcaatgg agttacgctg gcgaatcgcc
     1441 ctggtacgga acggctggtg cggcggttgt gaataaaggt gataaaacct ggcttattaa
     1501 tggcgaagcc aaaccaggat tgcgaacgga tgccgtattt gaacttgatt tcaccggtaa
     1561 taatttaaaa tggaataagc ttgatcccgt ctcatcacca gatggcgtcg ctggcggttt
     1621 tgcggggata agcaatgatt ctcttatatt tgctggaggg gccggattca aaggttcacg
     1681 agaaaattac cagaacggta agaactatgc gcatgaaggc ctgaaaaaat catatagcac
     1741 tgatattcat ctttggcata acgggaaatg ggataaatcg ggtgaattat cgcaaggtcg
     1801 ggcctacgga gtatcattgc cctggaataa tagtttatta attattggcg gtgaaactgc
     1861 aggcggcaaa gcggtgacgg attcagtttt aatttctgtg aaggataata aagtcacagt
     1921 acaaaactaa cgcttcaggg ccccggtaaa ggggccctgc tatcaatttg tcatttgtat
     1981 tacgcgaaaa ttatatgaac gcaataatat cacccgatta ttactatgtt cttaccgttg
     2041 ctggtcagtc taatgccatg gcgtatggcg aaggactgcc attaccggac agggaagatg
     2101 cgcctcattc cagaattaaa caattagcga gatttgcgca tacgcatccc ggaggcccgc
     2161 catgtcactt taacgacatt attccactga ctcactgccc acacgatgtt caggatatgc
     2221 agggttatca ccatcctctg gcaacgaatc atcaaacaca gtacggcacc gttggccagg
     2281 cactgcatat tgcacggaaa ttactgccct tcattcctga taatgcaggg attcttatcg
     2341 ttccgtgttg ccgtggcgga tcggctttta ccgcgggcag cgaagggaca tattcagaac
     2401 ggcacggagc cagccatgat gcttgtcgtt ggggaacgga tactccgctg taccaggatt
     2461 tagtcagcag aacgcgagcc gcactggcaa aaaatccgca gaacaaattc ctcggcgtat
     2521 gctggatgca aggcgaattt gacttaatga ccagtgacta cgcgtcacac cctcaacact
     2581 ttaatcatat ggttgaagcc tttcgtaggg atctaaaaca ataccattct cagcttaata
     2641 atattactga cgcgccgtgg ttttgcggcg ataccacctg gtactggaaa gaaaatttcc
     2701 cgcattcgta tgaagctatt tatggcaatt atcaaaataa tgttttagcc aatattattt
     2761 tcgtcgactt ccagcaacaa ggtgaaagag gactgacgaa cgcgcctgat gaagacccgg
     2821 acgatttaag cacgggatat tacggttcag cgtaccggtc accggagaac tggacgacgg
     2881 cactgcgaag cagtcatttc agcacggcag cccgtcgggg gattatttct gacagatttg
     2941 tagaagcaat tttgcagttt tggcgcgaaa ggtgagcggg gagtttattc atgggggatg
     3001 tccttgttcg ttctcagagt agacaacaaa aaacaaatga gatcaaggta atatctcttc
     3061 acttgcgggc tcgaacagca ggcaattgcc aatataataa tctggcacaa gggtgaaaaa
     3121 cgcgcaccgc ataaaccgtt gttattgctt acagattagc cggatgcctt aatagacatc
     3181 cgcgcctttt cgattatggc tcggaaatct acgaacatct acacagttta ctggaacgtt
     3241 ttgggccaca gcgttcgcag caccgtcctg atatgccgtt ctggcgatta taagtcgacg
     3301 gattctggca aatagacgat gatgatatgc ttaatgatgt tcaaagaaat taggccatta
     3361 ttatattatt gcttgcattt tctttcattc ctaaaagcac ccgccattaa tcccaggcgc
     3421 attatg
//
LOCUS       DAZPAB010000287         3414 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZPAB010000287 DAZPAB010000000
VERSION     DAZPAB010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.288"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..238
                     /locus_tag="ACHUPG_003954"
     CDS             <1..238
                     /locus_tag="ACHUPG_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFR0863336.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            248..886
                     /locus_tag="ACHUPG_003955"
     CDS             248..886
                     /locus_tag="ACHUPG_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR0863337.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            889..2052
                     /locus_tag="ACHUPG_003956"
     CDS             889..2052
                     /locus_tag="ACHUPG_003956"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR0863338.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2136..>3414
                     /locus_tag="ACHUPG_003957"
     CDS             2136..>3414
                     /locus_tag="ACHUPG_003957"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR0863339.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEG"
BASE COUNT          760 a          803 c          987 g          864 t
ORIGIN      
        1 attgttaggt tacgttatcc cattatccag tgcggcaatc aatgcggtga gtattccaga
       61 ctatttcgcc tggggcggga ttgcactggt gattcagtta ctcgtttttg ctggtgtcag
      121 actttatatg cccgcattaa gcgaaaaaat tattaatcac aataccgcag caggaatgtt
      181 tatgggaacc gccgcgctgg ctggcggtat ttttaacgca gcttgtatga catggtaatg
      241 gacgatcatg gccagaaaac gcaaatcaag aaataacagt aaaattggtc acggagcgat
      301 cagtcgcatt ggtagaccga ataatccttt tgaaccgcgt cgcaatcgct acgcacaaaa
      361 atacttaacg ttggcgctaa tgggcggtgc cgcttttttc gtattgaaag gttgtagcga
      421 tagtagcgat gtcgataacg acggcgacgg aacattttac gcgacagtgc aggattgtat
      481 tgatgacggt aataatgccg acatttgcgc gcgtggctgg aacaacgcca aagcggcatt
      541 ttatgccgat gttccaaaga atatgactca gcagaactgt cagtctaagt acgaaaattg
      601 ctactacgac aatgttgaac agagttggat cccggtcgtt tctggatttt tgttaagccg
      661 ggttattcgt aaagatcgcg atgagccgtt cgtctataac agcggcggat cctcatttgc
      721 ttcccgcccc gtctggcgca acacttccgg tgattactcc tggcgcttcg gttctggcaa
      781 aaaagagtct tactcttcag gcggctttac caccagaaaa gcgtcgaccg tttcgcgcgg
      841 cggctatggt cgttcttcca gcgcccgtgg gcattgggga ggctaatcat gctgagacac
      901 aacgttcctg tgcggcggga tctggaccag atcgccgcta acaacggttt cgactttcat
      961 atcatcgaca atgaaatcta ttgggacgag agtcgggctt accgttttac tctgcgccag
     1021 attgaagagc agatcgaaaa accgactgcg gaactgcatc agatgtgcct tgaggtggtg
     1081 gatcgcgcgg ttaaagacga agagatcctg acgcaactgg cgatcccgcc gttgtactgg
     1141 gatgtgatcg ccgaaagctg gcgcgcacgc gatccttcgc tgtatggccg gatggatttt
     1201 gcctggtgtg gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac gccgacctcg
     1261 ttgtatgagt cggcttattt ccagtggttg tggctggagg atgcccggcg cagcggcatt
     1321 attccgcgtg atgccgatca gtacaacgcc attcaggaac gcctgatttc gcgctttagt
     1381 gagctttaca gtcgggaact gttttatttt tgctgctgtc aggacaccga tgaagacagg
     1441 agtaccgtgc tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc gcggtttatc
     1501 tacattgaag atctcggttt gggcgtcggc ggcgtactga ccgatcttga tgataatgtc
     1561 atccagcgtg catttaagct gtatccgctg gagtggatga tgcgtgacga taacggtccg
     1621 ctgctgcgca agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat cttgagtaat
     1681 aaagggctaa tgccgctgct ttggcgcttc ttccctggtc atcctaacct tcttgcatcc
     1741 tggtttgagg gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg taaaccgatc
     1801 tactcgcgcg aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt cgttgaccac
     1861 gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc tctgccgcga
     1921 tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc gtgcggaatg
     1981 gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt tccgcattac
     2041 attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg cagtgaccat
     2101 actaatggtg actgccattg atggagggag acacagtgca ctggcaaact cacaccattt
     2161 ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc gcgctttgcg
     2221 aagcggtaac gcgtgaaggt gctggctggg atagcgattt tctagccagt attggtcagc
     2281 agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtaaac ccgcctgaac
     2341 tgttgcgcta cgatgcccag gggcggcgtc tggacgatgt tcgtttccat cccgcatggc
     2401 acctgctgat gcaggcgtta tgtaccaatc gggtgcacaa tcttgcctgg gaagaagacg
     2461 ctcgctccgg cgcatttgtg gcgcgcgcgg cgcgttttat gttacatgcg caggttgagg
     2521 ctgggtcgtt atgtccagta acgatgacct ttgccgccac gccattgttg ttacagatgt
     2581 tacccacgcc gtttcaggac tggaccacgc cgctgctgag cgatcgctac gattctcact
     2641 tattgccagg tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg gaaaagcagg
     2701 gcggttccga tgtcatgagc aacaccaccc gtgcagagcg tttggaagat ggctcttatc
     2761 ggctggtggg gcataaatgg tttttctcgg tgccgcaaag cgatgcgcat ctggtgctgg
     2821 cgcagaccgc gggcggtctg tcctgctttt ttgtgccgcg ctttttgcct gacgggcaac
     2881 gcaacgcgat tcgcctcgag cggctgaaag ataagctggg taatcgctct aacgccagtt
     2941 gcgaagtgga gtttcaggat gccattggct ggttattggg gcaggaaggg gaaggaattc
     3001 gtctgatcct gaaaatgggg gggatgacgc gttttgattg cgccctgggt agccatgcca
     3061 tgatgcgccg tgcattttcg ctggcgattt atcatgcaca tcaacgccat gtttttggta
     3121 atccattgat ccaacagccc cttatgcgtc atgtcttaag tcgcatggca cttcagcttg
     3181 aagggcaaac ggcgttgctg tttcgtcttg cgcgagcgtg ggaccggcgt gccgatgcca
     3241 aagaagccct gtgggcgcgt ttatttacgc ctgcggcaaa atttgtgatc tgcaaacgag
     3301 gtatgccgtt tgtggccgaa gcgatggagg tgctgggcgg cattggttat tgcgaggaga
     3361 gcgagctgcc gcggctttac cgggagatgc cggtaaacag tatttgggaa ggtt
//
LOCUS       DAZPAB010000288         3373 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZPAB010000288 DAZPAB010000000
VERSION     DAZPAB010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.289"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..1202
                     /locus_tag="ACHUPG_003958"
     CDS             <1..1202
                     /locus_tag="ACHUPG_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFR0863340.1"
                     /translation="TKDGQRLRNMIMSFNADIVARSPKKKPTFFPEQIEGYEYMYGGN
                     DDYPYYLQNRTDENGNDLPIGPISYMENPEVPQANAYMLEAATNAVKEVASLGVDAQA
                     ANGQVAFDTVNQLNMRADLETYVFQDNLATAMRRDGEIYASMVNDIYDVPRHVTLTLE
                     DGSEKDVQLYAQVVDYQSGNVVTLNDIRGRYECYTDVGPSFQSMKEQNRAEIQELLTK
                     VPQGTPEFQMLMLQYFTLLDGKGVEMMREYANKQLVMMGLKKPETPEEMEMVQQAQQQ
                     PQQPSAEQIQAQGILLQGQAELLKAENQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMD
                     LDKQAELREYLRLVGQFQQQRSKDARANAELLLKDADQTHSQRMDFANLMRQVQIPSG
                     GVAETPQ"
     gene            1216..2127
                     /locus_tag="ACHUPG_003959"
     CDS             1216..2127
                     /locus_tag="ACHUPG_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HFR0863341.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            2127..>3373
                     /locus_tag="ACHUPG_003960"
     CDS             2127..>3373
                     /locus_tag="ACHUPG_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFR0863342.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACA"
BASE COUNT          927 a          872 c          872 g          702 t
ORIGIN      
        1 tgacgaaaga cggtcaacgc cttcgtaaca tgattatgtc gttcaacgcc gatattgttg
       61 ctcggtcacc gaagaagaaa ccgaccttct tccctgagca aatcgaaggc tacgaataca
      121 tgtacggcgg aaatgatgac tatccgtact atctgcagaa caggactgat gaaaacggta
      181 acgacctgcc gattggtcca atctcctaca tggaaaaccc tgaagtgccg caagccaacg
      241 cttacatgct tgaggctgcc accaacgcag tgaaagaagt ggctagtctt ggcgtggatg
      301 cgcaggcggc aaatggtcag gtcgcttttg ataccgtcaa tcaactgaac atgcgggcag
      361 accttgagac atacgtgttt caggataacc tggctaccgc aatgcgacgt gatggcgaga
      421 tttatgcctc aatggtcaac gatatttatg acgttcctcg tcatgtaacg ctgacacttg
      481 aagatggaag cgagaaagac gttcaactct atgcgcaagt tgtcgattac cagtccggca
      541 atgtggtcac actcaacgac attcgcggtc gatatgagtg ctatacagac gttgggccat
      601 ccttccagag tatgaaggaa cagaaccgcg cagagattca ggagttgctc accaaggttc
      661 cgcaaggtac tccagagttc cagatgctga tgctgcaata cttcacgctg cttgacggta
      721 aaggcgtcga gatgatgcga gagtacgcga acaagcaact ggtgatgatg gggctgaaga
      781 aaccagaaac acctgaagag atggagatgg tgcagcaggc acaacaacag ccgcagcagc
      841 catcagcaga gcaaattcag gcgcagggta tccttctgca aggtcaggct gaattgctca
      901 aggcagagaa ccaacaggcg cagattcagg ttgaagctgc caaggttgaa gcccaaaacc
      961 aactcaacgc cgcgaagatt gcagaaatat tcaacaatat ggacctcgac aagcaggcag
     1021 aactgcgtga gtacctcagg ctcgtaggtc aattccagca acagcgcagc aaagatgctc
     1081 gtgctaacgc tgagctgctt cttaaagatg cagaccagac tcattcacaa cgcatggatt
     1141 tcgcgaatct tatgcgtcaa gttcaaatcc cctccggcgg agtagccgag acacctcaat
     1201 aagagagagt taatcatgga ccaaaccacc gacattcagg cttctgaaga attaaccctg
     1261 cccggcaatc atgcagcggc atctgctgat ggcttagttg tcgataatgc caacgacagc
     1321 gcaggtcagg aagaaggctt tgagattgtc cggaaagacg atgagaaacc aaaacaagac
     1381 ccggcaacta atgctgaatt tgcccgtcgc cgcatcgaac gcaaacgcca gcgtgagctt
     1441 gagcagcaga tggaagcggt taagcgtgga gagttgccgg agcacctgcg ggtgaaccct
     1501 gagttaccaa aacaaccaga ccctaacgat tatctttccg aagatgcact ggctaagtac
     1561 gactatgacc agagccgcgc actggctgcc ttccagcagg caaacagtga atggcagatc
     1621 aaggctatgg acgcacgaag ccaggctgtc gccgagcagg gtcgcaaaac tcaggagttc
     1681 acccagcaat cagcgcaata cgtcgaggcg gcccgtaagc actacgaggc agcggaaaag
     1741 ctcaatatcc ctgactatca ggagaaagag gatgcattca tgcaactggt gccgccagca
     1801 gtcggtgccg acatcatgcg cctcttcccg gagaaatccg ccgctctcat gtatcacctt
     1861 ggtgctaatc ctgagaaaac acgccagttg ctggcgatgg acgggcaatc cgcgctgatt
     1921 gaactcactc gactgtcaga acgtttaact ctcaagcctc gagccaagcc tgtttcagaa
     1981 gccccgttac ctgatgaacc cattcaggga catgctgttg ctgcaaatat ctctgcgatt
     2041 gaaaagcaga tggaggcggc agcaaacaaa ggggatgtag agacgtaccg caagctcaag
     2101 gcgcaactga ataaaggaat tcgataatgg cattaaatga aggtcaactg gtcacgtatg
     2161 ctctggatga aatcatcgga accgtccaga acctgacgcc aatggcgtca aaagtgacaa
     2221 aatacccccc tccggcagaa tccatgcagc gttcaagcaa caccgtgtgg atgcctgttg
     2281 agcaggaagc gccaacccag actggctggg atttaactgg caacgcaacc gggattctgg
     2341 aactctccgt gaagtgcaac atgggcgatc cggataacga tttcttcgag cttcgtgcag
     2401 atgacctgcg tgatgagcgt tcttaccgtc gccgcatcca ggcatccgcc aaaaaactgg
     2461 cgaataacat tgagtcagcg attgccaaac aggcaactga aatgggctcg cttgttgttc
     2521 acgatacccg cgcaattggt ccatcgactg gcctgcctgg ctgggatttt gtgtctgatg
     2581 cagagcgact gatgttctcc cgtgagctaa accgcgacat gggcatcagt tacttcctga
     2641 accctgacga ttaccgcaaa gcaggccgca acctggtaga tggtgacatc ttcgggcgcg
     2701 ttcctgaaga agcatatcga aacggtacta ttcagcgtca gattgctggc tttgatgaaa
     2761 ttcttcgctc accgaaactt ccggcagtta ccaagtcaac cgcgactggt gtaactgttt
     2821 ctggtgcgca gaagtttaag ccgcaggcat acacccttga taccgatggt aacaaagaga
     2881 acgtcgacaa ccgtgttgca acggtgaccg tatcctccac caccggattt aagcgcggcg
     2941 acaaaatcag cttcattggt gtgaagttcc tgtctcagat ggcgaagaat gtgctgactg
     3001 atgacgcgac tttctcaatc acccgtgtga tcgatggtac tcacatcgaa atcacgccga
     3061 agccgattgc gctggatgac gcgtcactga caaaagaaga gaaggcttac gctaacgtaa
     3121 acacctctct tgctgatacc actccggtaa acgttctgaa cgtggcaaca accaccgcta
     3181 acgtgttctg ggctgatgac tcaatccgcc tgctgtctca gccgatcccg gtaacccatg
     3241 aactgtttgc tggcatgaaa acgtcttcct tcagcattcc aggtattggt gttaacggca
     3301 tcttcgcaac gcagggtgat atcaacactc tgtctggtaa gtgccgtatt gctgtgtggt
     3361 attcagcatg tgc
//
LOCUS       DAZPAB010000289         3348 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZPAB010000289 DAZPAB010000000
VERSION     DAZPAB010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.290"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            19..897
                     /locus_tag="ACHUPG_003961"
     CDS             19..897
                     /locus_tag="ACHUPG_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0863343.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            894..2285
                     /locus_tag="ACHUPG_003962"
     CDS             894..2285
                     /locus_tag="ACHUPG_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR0863344.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2282..2539
                     /locus_tag="ACHUPG_003963"
     CDS             2282..2539
                     /locus_tag="ACHUPG_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863345.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2634..2834
                     /locus_tag="ACHUPG_003964"
     CDS             2634..2834
                     /locus_tag="ACHUPG_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFR0863346.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2827..3207
                     /locus_tag="ACHUPG_003965"
     CDS             2827..3207
                     /locus_tag="ACHUPG_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR0863347.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          885 a          726 c          969 g          768 t
ORIGIN      
        1 cattctgagg tgacagcgat gatgacgttt aacctgcgtg aacaacaaaa aagactacag
       61 gcgcgaatgg atgagttacg ggcagagatt gcatttgctc agaagggcga aaagccatgg
      121 ccttatcgtt cctgcctgat gcgtgaaggt cgcggatatt gcgaaaaaca tggcgaatat
      181 cacacgcata tactggtgtg gagcgatcgt aatggcgagg acagagaaga aatttcatgc
      241 tgccctgact gcttaatcgc tgaggccaac gatttgacca tggagctgtc gtccatcaag
      301 gcggaagagc tgactgataa cgccggaatt gccctgcgtt ttcgggactg cgagtttgat
      361 aattatctgg aggttaatcc tggcgcagcc agaaatcttg cggcctgtcg ccgctatgcg
      421 gagaactggc cagatatgct ggagaacggt accagtcttg ttatgaccgg cagttgtggt
      481 accggaaaaa atcatctggc tgtggctatg gcaaaacaca tcatccgtaa ctatctggcc
      541 agtgtggaga tcaccgacgt gatgcgcctt acccgtgctg tgaaaaattg ctggcggaat
      601 gacagcgaaa aaacagcgga tgaagttatt gagcgttatg cgtcaatgga tttgctgatc
      661 atcgacgaag ttggcgttca gtttggcagc gcggctgaaa tggctatttt gcaggaaatt
      721 atcaacgcca ggtacgaaag tatcttgccc accattctga tcagtaacct ctcaccggaa
      781 gagttgtggg cgttcatcag tccccgaatt gccgacagga tcacagacgg gggacgcaac
      841 tggttgtcgt ttaactggcc cagttaccgt tctcgtatca gaggtgtggc tgcatgacaa
      901 ctccagtatg gcgtaacgat gaccttgaag gcgctgtcat cggcgcgttc tttctgcgcg
      961 gagcagatcc tgaagtgatg gatattctgg ccacactgcc agcggacgtt ttttctgtac
     1021 gagcgtatca ggatatctat acaggcatct gcagacaggc ccgtgtttca ggagtgattg
     1081 accccgtgct gttgtgtaat gagatgccgg aacttgcccc ggtgattact gataccgggc
     1141 gtaaaacctg ggtgaagtct tcactggagc actatgttgc agcgttgcgg cgcaatgccg
     1201 cactgcgcga tgcagaaaaa acactgaatg aggcgctgca gaaattacgt gatgcgcata
     1261 cctgtgaagc agctgaagat gccctgaagg atgcgcagaa catgatggtc acactgtcga
     1321 caggaaaggg cgtcattcag ccggtacata ttgatgatgt gcttccggaa gtggttgagc
     1381 gtgttgaatg ccggaatcag gggctggaga aatccaggac gttgatgacc ggtattgatg
     1441 aactggacgc aaaaacaggc ggtatggagc ccggagacct ggtattcatt gccgcccgtc
     1501 cttcgatggg gaaaaccgaa cttgcgctgg acatcatcga caaggtgact gagcaggggc
     1561 atggtgtgct tctgttcacc atggagatgg cgaacatcca gattggtgaa cgtatggtgt
     1621 ctgcggctgg agggatgcca gtatcacgcc tgaaatctgt ggctcacttt gaagatgaag
     1681 actggacgcg tttctcacaa ggggtggggc ggatgaccgg gcgcaatatc tggatggtgg
     1741 accaggcgaa cctggccatt gatgagatat gcgcaacaac gaaacaccac ctgattaaat
     1801 atccggaaac ggcgctggtg gtggttgatt atctcgggct gataaaaacc cgaaccacgg
     1861 ggcgtcatga ccttgccgtg ggtgaaatct caaaggggct taaaggcctg gcaaaatccg
     1921 gtggttttcc gttgattgcg ctgagccagc tctcccgcgg tgtggagtcc agacccaata
     1981 aacgtcccat gaactcagac ctgaaaaatt ccggagaaat agaggcggat gcagacatca
     2041 ttctgatgct ttacagggat gaagtgtaca acccggatac gcaggccagg ggcatcgcag
     2101 aaatcaatat cacgaaacaa cgtaacggtt ctctgggaac gatttaccgg cgtttttata
     2161 acggacattt tctgcctgtg gaccaggaaa gtgcacgggt gctttccact cccatgaagc
     2221 ccggcaatcc gcgcagatac agtaataaac gaactgacag cagtaagatg gagcgtttct
     2281 tttgaacaac cagacaatga cttttacccc tgaacaattg cgtaaacacg cgcaggaaat
     2341 gctgcgtcat gctgaacaac tcgaaaaaac gggcataaca aaagatgcta tccgtaaaga
     2401 tatggtaccc gcgcttcggg aactgatgca ggcgaagcat cgcgcacaga aagcagtaga
     2461 tgagctggtg gactgtgtgg cagagctgga aaccagagtc ggaaagtttg aaaaactggt
     2521 gcaggaggcg ctgcgctgat gcgccatgag tttgttttgc cctggcttgt gcgcgatcat
     2581 tatgtccctg gtggaaagcc cgggataaaa atcaccgaac cggagagtat ttgatgagcg
     2641 tcaaaattca aacgataccg gaactgctta tccagacacg aggtaatatg accgaagttt
     2701 cacgaatgct gaactgtaat cgcgccacgg taagaaaata tgctgaagat aaagagggca
     2761 aagggcacgc cattgttgat ggtgttctga ttgttcatcg tggatgggac aggggtaaag
     2821 acagtgatgc gtgatattca gcaggttctt gagcgctggg gcgcgtgggt ggctaataat
     2881 catgaggatg tgacctggtc gtccattgct gccggtttta agggattaat cccttcaaaa
     2941 gtaaaatccc gcccgcaatg ctgtgacgat gacgcgatgg ttatatgtgg gtgtatggcc
     3001 cgccttaacc ggaacaacaa cgatctacat gacttgctgg ttgactacta cgtgtttggg
     3061 atgacattca tgacgctggc gcggaagcat ggatgctcag atggttatat aggaaagaag
     3121 ctacaaaaag ccgagggggt ggtggaagga atgttgatga tgttgggtgt ccggttagaa
     3181 atggacaaat atattgaaca tccctgatgg taaggggcga ctttctagaa aatattttac
     3241 gtacgtaaaa aacgggatat tctgttaaga gtggttgctt cgccacacag cttaaacccg
     3301 cctccgggcg ggttttttat gcctgaaaaa cggtacagga cgttaaac
//
LOCUS       DAZPAB010000290         3326 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZPAB010000290 DAZPAB010000000
VERSION     DAZPAB010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.291"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..209
                     /locus_tag="ACHUPG_003966"
     CDS             <1..209
                     /locus_tag="ACHUPG_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863348.1"
                     /translation="RHPDKRFIFNGNEIWKRGESTTEYTRAHQRTIRRLRRLIARKQY
                     TEKWFRKYDTWSAGYYELMATKPF"
     gene            270..458
                     /locus_tag="ACHUPG_003967"
     CDS             270..458
                     /locus_tag="ACHUPG_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863349.1"
                     /translation="MNIYFRIVISLAIIACIYGLIVPALISMKDTVAVISGFALACLT
                     PPCIYAIYKGLSFSKDKR"
     gene            455..1282
                     /locus_tag="ACHUPG_003968"
     CDS             455..1282
                     /locus_tag="ACHUPG_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR0863350.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1326..2075
                     /locus_tag="ACHUPG_003969"
     CDS             1326..2075
                     /locus_tag="ACHUPG_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR0863351.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2072..2641
                     /locus_tag="ACHUPG_003970"
     CDS             2072..2641
                     /locus_tag="ACHUPG_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863352.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2638..2760
                     /locus_tag="ACHUPG_003971"
     CDS             2638..2760
                     /locus_tag="ACHUPG_003971"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863353.1"
                     /translation="MKIEYQDAAGGESMSWPDAIVTLGMVFAVAFVVYSICRWG"
     gene            2765..3007
                     /locus_tag="ACHUPG_003972"
     CDS             2765..3007
                     /locus_tag="ACHUPG_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFR0863354.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3011..3157
                     /locus_tag="ACHUPG_003973"
     CDS             3011..3157
                     /locus_tag="ACHUPG_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863355.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            3166..>3326
                     /gene="xisR"
                     /locus_tag="ACHUPG_003974"
     CDS             3166..>3326
                     /gene="xisR"
                     /locus_tag="ACHUPG_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFR0863356.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPKP"
BASE COUNT          929 a          797 c          854 g          746 t
ORIGIN      
        1 aacgccatcc tgacaagcgt tttatcttca acgggaatga gatatggaaa cgtggagaat
       61 caaccacaga gtatacccgc gcacaccagc gaacaatcag acgactgcgc agactcatcg
      121 ccaggaaaca gtatacagaa aaatggttca gaaaatatga cacatggagc gccggatatt
      181 acgaactgat ggcaacaaaa ccattctgac gtaaacgaaa ttaaccatga cgcaattaaa
      241 taaggcaagc cgaatacatc aggaggacca tgaacattta tttcagaata gttatatcac
      301 tggcaattat cgcatgtatt tacggattaa tcgttccggc cctcatatca atgaaggata
      361 cggtagcagt gatttctggc tttgctctgg cgtgtctgac cccgccctgc atttatgcca
      421 tttataaggg tctttctttt tctaaggata aaagatgaaa aaagttattc ctgctttagc
      481 ccttgttctg ctgaccaccg gacttgtcgg ttgcgatcgc gttgagcctg gtaatgttgg
      541 catcaaggta aacaaactgg gcgacgataa aggcgtcgga gaagtggtcg gtgttgggcg
      601 ctactggacg ggatggaaca ctgaggttta catcttcccg accttcaaac aaatgaagac
      661 ctacgatgag ccattcagct tccagatgag tgacggtaca accatcggct atcacatcgg
      721 cgtggcctac aaagttgatc catccaaagt taccacagtc tttcagacct accgcaaagg
      781 cgtggacgac attaccgaca ccgacctgcg ccagaagata gccgacgcac tcaaccgact
      841 ggccagcaaa atgaccaccg acaaatttat cgacggcggc aagtctgagc tgctggatgc
      901 agctcttaaa gacattcagg aagaaatgac gcccatcggt attcaggtaa tgagcctctc
      961 atatgtgggt aagccggagt acccgcctac tgttatcgac agcattaatg ccaaagtcac
     1021 ggcgaaccag aaaaccctgc aatgcgagca ggaagtaaaa cagcgcgaag cggaagccaa
     1081 catgttgcgc gcggaagctg ccggacaggc agatgcgatt cgcacaaaag cccaggccga
     1141 agccgatgct attcgtttac gcggtgaagc tctgcgccag aacccaaacg ttatggagct
     1201 ggaagccatc aacaagtgga acggtacact gccgcaatac atgaccagcg gtgccaacac
     1261 accatttatt caggttaagt aatacatatg cccggtatta cacgccgggc tgtctggaga
     1321 taaaaatgaa tactgtaacc atcaacaaca aacagtttcc ggtaatcgaa tatcgcggtc
     1381 agcgtgttgt gacgctggcg atgattgatg aagtccacca gcgaccggaa ggaaccgcgc
     1441 gtgcagcatt caaccgaaac cgtgagcatt tcatcagcgg tgtggattat gccgaattag
     1501 gtgcggacgt aatacgtacg gacctcccgg aaggaacatt ctctaaattt gcaccatcgg
     1561 ggattgtact ttttgaatca ggttacctga tgttgacgaa gccattcaat gacgcccttg
     1621 cctggcaggt tcagcgcgaa ctggttaaca gctacttccg cactcgcaca ccgctaacgg
     1681 aaatcgagat gatcgctgcg atagccgccg acgctgttcg tcaacagaag cgcctgaatc
     1741 atgtggaaga acagctcgaa acagtcacag aagcggtgga aaccatcaaa cgcggaaata
     1801 tgcgcgccgg atatgtcggt taccgccagg tggtagccaa aagtggaatg agtgacacca
     1861 agtgtcggaa tctggtgaac gcatatcaaa ttcctacaga cacccacgaa ttcatgactc
     1921 cggacggtct gttgtctcgc agggctatcg tcgagcttga gccatttatg gcggcgttcc
     1981 accaaatgat gtcagaggct gacccacgcg gcacacgctg gtatcacccg aagatggggc
     2041 ttttccaggt gatcggatgg gaggataaag catgatcatc cagtcaaaac ttattcgcgc
     2101 cgctctggtg tgcgctgcta aaaacgacgt tcgttattac ctgaacggtc ttcacatcac
     2161 gccgaaacat attgaggcaa ccaatggttc cgtggcactg cgcatggctc acggcatccg
     2221 gacgaagaaa aacatcattg tccagttcga agggggcgtc ccggccaaag ccgaaacgac
     2281 agagctgatt tttagtaaag agccgatcgc tgttcatcgc gaccagtttc agcgacgact
     2341 gtccattacc ggcattaaac tggtggacgg ttgttttccg gatttggatc gcatcattcc
     2401 gaaaaagttt gaccgctgta ctcacccggt gctccaggcg ggttacctga gttatccaga
     2461 gaagatgttt ggtcgtgagc gtaaatttat tcccgtccag ttacgtccct ccggtgacgg
     2521 gcaagcggtc agaattcagt ttgattccat catcaactca atgtatggca atcctgaatt
     2581 tgttgtgatg ccttgtcgtg atcatggcga tttcaacgtg gctcaggagc atccggaatg
     2641 aaaatcgaat accaggacgc cgcaggaggt gaatcaatga gctggcctga tgcaatcgta
     2701 actctgggga tggtattcgc agtagcgttt gttgtgtact cgatttgtcg atggggataa
     2761 ccacatgttc gctttgattc aacgcgggca gatatacaca gacagagccg gatacccagt
     2821 agaaattact cgcagtactg agcactcagt gttctttcgg aggatggacg gacgtaccgg
     2881 gcgagtacgc atcggtgaat tcagtagcct gttcgagcat attgaccacc tggaatatca
     2941 caagattctg gctgaaacag aacaagaaaa gcacctgaag aaattacgcg caatgcaaag
     3001 gaagtaaaaa atgaataaag cgtttgagct atgggtccac cagcgttacg gcaaccacta
     3061 cgacctgacg cgagatgttg acggtttcta ctgtcgtgaa attgtgaaac gaatgtttga
     3121 ggtctggtgc cactgccgtg ggctgagtgt tgtgtgaggt aatgcatggg caatgtgatt
     3181 caactggctc ccaatgaatg ggtttgtgaa agcgttctaa tcgcaattac cgggctcaaa
     3241 ccaggcacaa ttcttcggac ccggaaagaa tgctggatgg ttggaagaga gtatattcac
     3301 gtatcaccag acggtaatcc aaagcc
//
LOCUS       DAZPAB010000291         3280 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZPAB010000291 DAZPAB010000000
VERSION     DAZPAB010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.292"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..263
                     /gene="yobA"
                     /locus_tag="ACHUPG_003975"
     CDS             <1..263
                     /gene="yobA"
                     /locus_tag="ACHUPG_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR0863357.1"
                     /translation="AQVTAAPQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNE
                     QDQKQLIVPLADSLKPGTYTVDWHVVSVDGHKTKGHYTFSVK"
     gene            267..1139
                     /gene="copD"
                     /locus_tag="ACHUPG_003976"
                     /pseudo
     CDS             267..1139
                     /gene="copD"
                     /locus_tag="ACHUPG_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            1152..1493
                     /locus_tag="ACHUPG_003977"
     CDS             1152..1493
                     /locus_tag="ACHUPG_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR0863358.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1886..2963)
                     /locus_tag="ACHUPG_003978"
                     /pseudo
     CDS             complement(1886..2963)
                     /locus_tag="ACHUPG_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2929..3210)
                     /locus_tag="ACHUPG_003979"
     CDS             complement(2929..3210)
                     /locus_tag="ACHUPG_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR0863359.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT          783 a          750 c          751 g          996 t
ORIGIN      
        1 acgcgcaagt gacagctgcc ccgcaggcaa tcactttaaa cttctcggaa ggcgttgaaa
       61 caggattcag tggtgcaaaa atcacggggc caaaaaacga aaatattaaa acattaccag
      121 cgaagcggaa tgagcaggac caaaagcaac tgattgttcc actggccgat tcgctgaaac
      181 ccggaaccta caccgttgac tggcatgttg tttcggtgga cgggcataaa acgaaaggac
      241 actacacctt tagcgtgaaa taaatcatgc tggcgtttac ctggatcgcg ctgcgattta
      301 tccatttcac ctcgttgatg ctggtctttg gctttgcaat gtatggtgcc tggctggccc
      361 ccttaatgat tcgtcgcttg ctgacgaagc gttctctgcg tttacaacag cacgctgccg
      421 tctggagttt gatcagtgcc actgcaatgc tcgccgttca gggcgggttg atgggaacgg
      481 gatggacaga tgtattttcg ccaaacattt ggcaggctgt tttgcaaacg cagtttggtg
      541 gcgtctggtt atggcaaatt gttctcgccc tcgtaacgtt gatcgtcgcc cttatgcaac
      601 cgcggaatat gccacgtttg ctgtttatgc tcactaccgc gcaatttatt ctgctggcgg
      661 gagtggggta tgcgacgctg aatgaagggg taacagcgaa aatccatcag actaatcacg
      721 cgattcacct gatttgtgcg gcggcgtggt ttgggggatt actgccggtg ctctggtgta
      781 tgcaactcat caaaggccgt tggcgacact aggctattca ggcgctgatg cgtttttcct
      841 ggtgcgggca ttttgcggtg atcggcgtac tggcaagcgg cgtgcttaat gcattgttga
      901 ttaccggatt tccccccacg cttacgactt actggggcca actcttattg ctcaaagcca
      961 tcctggtgat gattatggtg gtcattgcgc tggctaatcg ttatgttctt gtaccgcgca
     1021 tgaggcagga tgaagatcgt gcggcaccct ggtttgtgtg gatgactaaa ctcgaatggg
     1081 cgataggtgc ggtagtgctg gtgatcatca gcctgcttgc gactctcgaa cctttttgat
     1141 ggattacgaa aatgatgaaa aaaagtattc tggcgtttct gttactcacc agttctgcag
     1201 cggcgctggc ggctccacag gtgataaccg tgagtcgttt tgaagtgggt aaagacaaat
     1261 gggcgtttaa tcgcgaagag gtaatgctga cttgccgacc gggtaatgct ttgtatgtca
     1321 tcaacccaag taccctcgtg cagtatcctt taaacgatat cgcacaaaag gaagttgcca
     1381 gtgggaagac caatgcccaa cccatttcgg tgattcagat tgatgatcct aacaatcccg
     1441 gcgaaaaaat gagtctggca ccgtttatag aacgagctga aaaactctgt taattaccta
     1501 aaatagcctt ttgatttcca ataaaaaaac cgcctcagtt ctttcaccag aacgggcggt
     1561 ttttaacatt taagctgatg agcaccgcgc tttttattga ccattttgca cgcaaactgg
     1621 aaaacctggc gtcgtcatct attcttaaag ggcaaggcaa ctaagcctgc attaatgcca
     1681 acttttagcg cacggctctc tcccaagagc catttccctg gaccgaatac aggaatcgta
     1741 ttcggtcttt ttttaattgt atttaaaatc aattagttgc aaacgtctcc ccgaaattcc
     1801 ccgaaattta ctcgaatttc tgtattccgg tcttttttgg ttatatcaca accaaaatac
     1861 atttaacaat ccatttacgt taaaatcaga gcagtaagta cgttttttct ctctcatcaa
     1921 gatacatttt tgttgtcttc tccgatgtgt ggccaagtag acgctgagca aattcttctc
     1981 cacatatttc tttgtacaat cgtccagcca ggcttctgat ttcgtgaaaa gttggtgggt
     2041 tttcactgaa cagtgatacc tgttaatttt ctggctgcga caaatttctt tgtcaggccg
     2101 tccgggtgaa tgctgccgtc agggctgttt tttctaatcc cggcactgat tagataatcc
     2161 cccctactta ccatgcggca ctgttcaact actgcaccaa gtcgtaaacc agcgacagga
     2221 aggctgagtg acagggggat agcaatcatc attcctgtct taatttgcct gatgtggaga
     2281 cgatcatcat aaatatcact gaaccgcata ttcgttatgt cttcgcgacg ttgtcctgtt
     2341 acaagggcta agtccatagc taatgggaac catgccggaa gttgatctgc tgcctccctg
     2401 atgcagttgt atgtctttag tttcagccgt tctcttgtaa ctactatttt cggtgctctt
     2461 gttggcgtta ctggattttg agatatacgt ccttcaacaa tggcttcgcg gaacatatca
     2521 gataacacag aacgcattga tcctgccatc gtgtttttcc ctccttcaat ctacaaatca
     2581 agaaactcgg cgatatggcg agtggttatt tctgtcagta aaacccctcc taatttttct
     2641 tttattgtct ccagttgatt tacccgagtt ttataagtat ttctggacgc ttttctcctt
     2701 ataagaatcg ttttgtaacg ttcaatccag cctgccatag taaatgagtc gaccccttta
     2761 agcttttcaa ttagggcagc aggagagtag tttttgtata tataatgatt tgcttcaatt
     2821 gcctgcgcta ctgcatctct tcttgaaatt ttacctagtg taaattcttc tttcgtcaga
     2881 gggttgcgcc agtaatatgc tttgtctctc cttcgatatg ttaagtttct aggcaaattg
     2941 ggatcgtatt ttttccgctg catgttttaa cttctccatt aacggactgt ctctcccttg
     3001 tcgcccatta ggctgatggt gtgttatatc ggtatcaacc ttatttgggt tgatatagaa
     3061 agcctccgga accaccctgt aactcctccc gtgtagttca ggtgcaggat aaatgtttcc
     3121 attccttgcc catcgtctca gcgttgatat tgatggtggg ttatccggat atctgagttt
     3181 tccccacgtt ttgagtgtca caagattcat tgccatacct ctcacgatat gaccgccagt
     3241 aaatatacag aatactggcg ggtgtggttg atttttaata
//
LOCUS       DAZPAB010000292         3262 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZPAB010000292 DAZPAB010000000
VERSION     DAZPAB010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.293"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(139..993)
                     /locus_tag="ACHUPG_003980"
                     /pseudo
     CDS             complement(139..993)
                     /locus_tag="ACHUPG_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            1101..1379
                     /locus_tag="ACHUPG_003981"
     CDS             1101..1379
                     /locus_tag="ACHUPG_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863360.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1380..1907)
                     /gene="gadE"
                     /locus_tag="ACHUPG_003982"
     CDS             complement(1380..1907)
                     /gene="gadE"
                     /locus_tag="ACHUPG_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR0863361.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2706..>3262)
                     /gene="hdeD"
                     /locus_tag="ACHUPG_003983"
     CDS             complement(2706..>3262)
                     /gene="hdeD"
                     /locus_tag="ACHUPG_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR0863362.1"
                     /translation="ATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFVSGDILS
                     TVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPELGIFAIA
                     AFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVSVTLVST
                     LVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT          923 a          724 c          701 g          914 t
ORIGIN      
        1 tttatcgata tcgaagcatt gagcattttt aactcgtttt atcttgaatg caatcggcgc
       61 ataacttatt gaatcccatc aaatcccgtc tttttttacc cattcccggt tatcttgaat
      121 aaattaccta gtcataaata cattctggcg gctaccttca tccactaatg ccgtgacata
      181 catgccaggc agcaagtcac catttgggtt ggggaaaatc gcccgtaacg tcacggagcc
      241 cgtggtttca tcaaccgtcg ggtcggagaa tttcagcgtg ccggtctggc tgtaacgttt
      301 accattttcc agattgagct gtactggcgt actgccctga acctgtttga tttgcccact
      361 ggcgacctct tctttcatgc gtaagaaatc ttgcaccgac tgcgtgagat cgacataaat
      421 cgggtccaga cgttgtacgg taaccagcga atctgcctga ttagcggtaa cgagtgcgcc
      481 gacggtcacc gacgatttcc cgctgacgcc cgtaatcggc gaggtgacat tcgcgtattg
      541 cagattgatc gtcgcctgtt caacagccgc tttggcgacg gtgacattgg cttctgcttc
      601 attcagctgg gtgcgcgcgg tgtcgtaatc ctgacggcta acgtagttgg tcttcagcaa
      661 cgatgcctgg cggttaaagg tgatgcgggc attgctggcg gtagagagcg ctttcgccag
      721 cgagcctttg gcggagttta gctcggcctg taaaggtgca ggatcaatct gatacagcga
      781 atcgccctgg ttcactttat cgccttcgat aaagttgcgt ttaatgataa taccgcccac
      841 ctggggacgt atctcggcaa cttcataagg aacggttcta ccgggcaatt cgctcaacac
      901 attgaccgaa ccaggggaga gtgtgacgac accgacctca ggcgtcatgg cggcggcgtt
      961 ttccgccgat ttgtcatcgc aggcggtgag catcgcgccg cagaataaca acggtattaa
     1021 cagctttctt cttctgttca ttttagtccc tgaaaattct tgagaaataa cggttcttta
     1081 aattgcgcgc gaggagagat atgagggatt tacagaccag cggcatagtg gggttgtcag
     1141 ccagtaaggt gggatacagg cacagtgatc gacatggtga ggtcaacgac aaaaaaaatg
     1201 ttgccgttct gccaacagtt cctgccagca ttcgggcaac gaagggttct actggtggat
     1261 acacatacca ggggaatcaa gtcgttttag cctctccttt agtaatcacc gggggtaatg
     1321 aaatcagcat ctgcatccct cgtcatgcca gccatcaatt tcagttgctt atgtcctgac
     1381 taaaaataag atgtgatacc cagggtgacg atgtcgctca tacgtcttag tttcagcttg
     1441 tacataatgt tgaatttctg aatctttaat gtttgttgcg aaatattgag cgtcgacgtg
     1501 atattgcttt catttttatt tttcagaatc agttgcataa ttttgaactg attatgtgaa
     1561 aaacataatt gtgaattctt atggggcaag tgtttaccat aagtaatctc aagaagattt
     1621 ttgatacttt ctttgcggct aacaaaataa ataggcgctt tagcttttag tttactgatg
     1681 tgataaggtg ccaaaacaat aattctctcg gcatctaatt tctccagaaa tttaatcgct
     1741 tcttcatcaa ggatatgatt gtgataggta tcaatgataa ccttgaaggg tttattgcct
     1801 acttttttga tctctgacag ggaattgatt tttatcattt cgtgattatc tttcaactgc
     1861 caaaagccct gtaaaagaaa agaatctttc gtcatgagaa aaatcataac ttgctcctta
     1921 gccgttatcg tttcgaacat gtcatccttt tcattattta catccttgtc cgaatcgttg
     1981 ttcaatatag taaacgccta taagaacttt gcccttcgtg aactaatacg tctattaaca
     2041 ctaatcaata cttcagattt atccatcacg cactccatat attgatccta agtaagattc
     2101 ctggttgtta tcagcttgta aatagtaaac aaaaagttaa cgtttgcgga ttactgccca
     2161 agaataagtt atcaataacc agtttgtgat ctctgaagaa tattactaaa gttaaaatgt
     2221 aatccgattt aaatatcgag tctccttgtt tcgacttaag ctggcaattg gattgccagc
     2281 tttcttttaa cgcattgaaa ttacctgagt aaataacgca ttcatgtctt caggttttta
     2341 tatagcgtct ggagacacgg atatttatgc aatgtaatca acgccagttg caaaaaaatg
     2401 aacccggatt acttatttaa ccgcccatag catcaacgct tacggaactc tttgttcact
     2461 ttcccacagc acttttaaaa cattaaacct gactacggaa aatatcagcc atgataatgt
     2521 ttgaaatggc taatttgcca tggagtgaaa aaaattagat gaaattcagt aggttgaaat
     2581 aatcactagc aggtaattat ttcaatgata gtgcgcaatt gatctacaac actgcgtagc
     2641 ggagagagta ttaatcggat catagtcaca tcaagtgact atgatccggg tgacaaccgg
     2701 ggtaattatt gctgcttaac gaacaaactg gcgaagctga acaggctggc ggcgctaaat
     2761 atcagttcaa ttcccaccag tgtggaaacc agcgttacag acaccatcgg cgttgcacca
     2821 aggaatatcc aggcaatgac gatatccagc acaccaataa cgagctgtag ccagctgcct
     2881 ttcattgaac gctgacgata ccaactcatc aggcgaataa cccctgcaac acagaacaaa
     2941 ccggcaataa atgccgcaat ggcaaaaatg cccagctccg gtgcacggat gaagaaatag
     3001 ccgatcaata aataggcgac tgcgacgagg aaaccggata ataccggcca gaaattatga
     3061 ctgcggttgc tgaataaccc gacaataagc gcaatacccg agcagattaa taatgcaccc
     3121 actactgtgc ttaaaatatc gccagagacg aacgggaaac tgatacacag caacccgacg
     3181 ataaacagca gcacggcaat aaactggatt gctctgcgat gctttttcag catctccaga
     3241 tcaaacttca aaattgttgc ct
//
LOCUS       DAZPAB010000293         3234 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZPAB010000293 DAZPAB010000000
VERSION     DAZPAB010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.294"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(2..298)
                     /locus_tag="ACHUPG_003984"
     CDS             complement(2..298)
                     /locus_tag="ACHUPG_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFR0863363.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            513..1241
                     /gene="tonB"
                     /locus_tag="ACHUPG_003985"
     CDS             513..1241
                     /gene="tonB"
                     /locus_tag="ACHUPG_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR0863364.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="ACHUPG_003986"
     CDS             complement(1281..1679)
                     /gene="yciA"
                     /locus_tag="ACHUPG_003986"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR0863365.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1784..2323)
                     /locus_tag="ACHUPG_003987"
     CDS             complement(1784..2323)
                     /locus_tag="ACHUPG_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR0863366.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2353..3096)
                     /locus_tag="ACHUPG_003988"
     CDS             complement(2353..3096)
                     /locus_tag="ACHUPG_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR0863367.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(3124..>3234)
                     /locus_tag="ACHUPG_003989"
     CDS             complement(3124..>3234)
                     /locus_tag="ACHUPG_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863368.1"
                     /translation="PTPEGQQLPPLYKDMLFHTRPDAATRGLTRVQLPAN"
BASE COUNT          920 a          808 c          776 g          730 t
ORIGIN      
        1 ctcagaacac tttcttaaat ggtttcactg aaacgtgttc atagactcct gccgctacgt
       61 acgggtcagc atcggcccag gcctgagctg cttccagcga ttcaaattca gcaataacgg
      121 ttgagccagt aaatcccgca gcccctggat cgttactgtc taccgctggc attggaccag
      181 ctgtcaacaa acgaccttca tcatgcagta actgtaaacg tgctaaatgt gccggacgaa
      241 cggaaaggcg tttttcgagg ctatcagctt tgtcttgagc gtaaataaca tacaacacgg
      301 gcacaactcc ttgttcggga aagttgtaaa gtacgttatt tgaaagggcg aagatctgca
      361 acggaaagat gatgtctttg ttaaggtcat gcataaagta agggtaatta cgccaaaaat
      421 gacattttca ctgatcctga tcgtcttgcc ttattgaata tgattgctat ttgcatttaa
      481 aatcgaggcc tagtttttct actgaaatga ttatgacttc aataaccctt gatttacctc
      541 gccgcttccc ctggccgacg ttactttcgg tctgcattca tggtgctgtt gtggcggggc
      601 tgctctatac ctcggtacat caggttattg aactacctgc gcctgcgcaa ccaatttctg
      661 tcacgatggt tgcgcctgct gatctcgaac cgccacaagc cgttcagccg ccaccggagc
      721 cggtggtaga gccagaaccg gaacctgagc cgatccccga accgccaaaa gaagcaccgg
      781 tggtcattga aaagccgaag ccgaaaccta agccaaaacc gaagccggtg aaaaaggtac
      841 aggagcagcc aaaacgcgat gtcaaacccg tagagtcgcg tccggcatca ccgtttgaaa
      901 atacggcacc cgcgcgcccc acatcaagta cggcaacggc tgcaaccagc aagccggtta
      961 ccagtgtggc ttcaggacca cgcgcattaa gccgtaatca gccgcagtat ccggcacgag
     1021 cacaggcatt gcgcattgaa gggcaggtta aagttaaatt tgacgtcacg ccggatggtc
     1081 gcgtggataa cgtacaaatc ctctcagcca agcctgcgaa catgtttgag cgtgaggtga
     1141 aaaatgcgat gcgcagatgg cgttatgagc cgggtaagcc aggcagtggg attgtggtga
     1201 atatcctgtt taaaattaac ggcaccaccg aaattcagta agcagaaagt caaaagcctc
     1261 cgaccggagg cttttgacta ttactcaaca ggtaaggcgc gaggttttcc ttcaggatca
     1321 accgctacat acttaaataa tgcttctgtc gctttatagc gttgcccgat tggttcagac
     1381 gctacttttt tcacccacac ttcaatatta atgctgaccg atgtcgtccc tttctggaca
     1441 cagcgtgcat agcagcacac cacatcgccg accgcaaccg gccgtaagaa agtcattcct
     1501 tcaacccgca cagtcactac gcgaccgtgg gcaatttctt ttgccagaat agcgccgcca
     1561 atatccattt gtgacattaa ccaaccacca aagatgtcac cattggcatt ggtatcggcg
     1621 ggcatggcta aagtacgtaa aacaagatcg ccctgaggga cgttatgtgt tgtagacatg
     1681 gtaaaaccga cttaaaagga aaatcacagg cgcgatgcta ctatgatttt cctgaggaga
     1741 acagaggaga tacagcgcca gccccgaagg actggcagtc tggttaggat ttatcttcct
     1801 gcggcatgtg gcggtagata tagataccgc ttaacaatgt gaagattaag gtaagggcgg
     1861 tcaggccaaa gactttaaag ttgacccaaa tattttgcgg cagccagaat gcgatgtaga
     1921 tgtttgccag accgcaaagg ataaagaaaa cagcccaggc cagatttagc ttcgaccata
     1981 ccggttgcgg cagcgtgagt tctttactca gcatccgctg aattagcggc tttttcatca
     2041 cccattggct gactaacagg gctcccgcaa acagagcata aatgactgta accttccatt
     2101 taataaactc atcattgtgg aagaacagcg tcaagccacc gaagacgacc accagaacaa
     2161 aagtgatcag ggccatcttc tcaaccttac gaaagcgaac ccagctatag ataagcacaa
     2221 tcgccgtggc gacgatcagc gctgcagtag ccgcatagat gtcataaatc ttgtaaaacg
     2281 cgaaaaagac aaccagcggt aaaaaatcaa gaaactgctt cattttacga ttccgtaatc
     2341 aaatgcttaa ggttattggc gaatcaacat atacaggcgg aacagataga tgagcaatat
     2401 ggcagaaatc aggttgctca aggtgttcgc cagtacggca ccaatttccg gggttaatgc
     2461 ggcaaaagaa gaggcaaaaa gcagcaacag tgtttttgcc agcaaccagc tcagtactgc
     2521 gggtgccacc agacgcatat tcgcccaagt cagccgcata ctgctacgca tcgaggcaaa
     2581 aatgcccatt ttgtcctgta ccagcatcac cggtgccagg gccagtaaaa tggccatgat
     2641 aattcccgga acgaccacca gcatgatgcc aatctgtact aaaagggtag tcagaaaaat
     2701 cagaataaat aacttgggca atatcggcgc actggcacca atagctcgca gcgcactgac
     2761 tctctgaccc gcagacacca gctggataat taatattacg cctccggcga gaatggcgtt
     2821 accgattaat tctgaaaaag tggacgccgc tgaagcctgc agcaaaattt gttgctgttc
     2881 cggtgacata ttctgaacca ggtcgaacaa cccactactg ccgctaacgg gcacgccgtc
     2941 attgagctgc gcaagctgtg catcactggg tgagaaaaca tgccctaaca ccactgtgat
     3001 aaacgcacat agcaacgata ccaacagaat ggtcataaat tgattacgga agaaatttcc
     3061 ggtgtcacgg tatacggact gcgccgtgat agacatgcac tctccttgag ttttatgcag
     3121 gtgttaatta gcgggcaatt gtaccctggt taagcctcgg gtggcagcat caggtcttgt
     3181 atggaaaagc atatctttgt aaagcggagg taattgctgg ccttcaggcg tggg
//
LOCUS       DAZPAB010000294         3205 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZPAB010000294 DAZPAB010000000
VERSION     DAZPAB010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.295"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(43..669)
                     /gene="dsbA"
                     /locus_tag="ACHUPG_003990"
     CDS             complement(43..669)
                     /gene="dsbA"
                     /locus_tag="ACHUPG_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR0863369.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(686..1672)
                     /gene="srkA"
                     /locus_tag="ACHUPG_003991"
     CDS             complement(686..1672)
                     /gene="srkA"
                     /locus_tag="ACHUPG_003991"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR0863370.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1749..2018)
                     /locus_tag="ACHUPG_003992"
     CDS             complement(1749..2018)
                     /locus_tag="ACHUPG_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR0863371.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2088..2672
                     /gene="mobA"
                     /locus_tag="ACHUPG_003993"
     CDS             2088..2672
                     /gene="mobA"
                     /locus_tag="ACHUPG_003993"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR0863372.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2654..3181
                     /gene="mobB"
                     /locus_tag="ACHUPG_003994"
     CDS             2654..3181
                     /gene="mobB"
                     /locus_tag="ACHUPG_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR0863373.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          842 a          777 c          769 g          817 t
ORIGIN      
        1 aagcccgtga atattcacgg gctttatgta atttacattg aattattttt cctcggacag
       61 atatttcacc gtatcagcat actgctgaac aaaaacatcc atattgctgg tatccatacc
      121 ctgcggattc agctgatatt taccgttaac aaacatcgcc ggaacaccac gcaattgcac
      181 atcagctgcg gctttttcct gctgagcgac cagagatttc accacgaagc tgttccacgc
      241 cgcgtcgtac tcttcacctt taatacctgc attgataaat acatcgcgga tatcagatgc
      301 tgaacgaatg gtctgggttt tctgtacgcc ttcaaacagc ggaacagtca ctttgtcttc
      361 cacgcccagc gccatcgcca cagcccatgc ctgagtcaga tctttgccca ggtcaccacc
      421 catgaagttg acgtggtatt tagtcatctt cacgccttcc ggcagttttt tcttcacatt
      481 atcagaaata tgcagaactt cttcaaactg atagcagtgc gggcagaaga aagagaaaaa
      541 ctccagcact tgcggcgcgc cagctaccgg tttttccagg gtagtgtact gtttaccatc
      601 ttcatactgc gccgccgatg cgctaaacgc taaaactaaa ccagccagcg ccagccaaat
      661 ctttttcatg atctactctc tccgattaat acataggtgt taattgcaaa gggggttctt
      721 gtagaacttt tgcctgttct ataaaagtcg ccgtctgtcg tagccagtaa tcttccccgg
      781 ttaaccacgg gaaatttttc gggaacgcgg gatcagccca atgccgcatt agccaggcaa
      841 gataataaac caaacgcatg gcgcgtaaag gttcaatcag tccgatttca gcggtgtcga
      901 actcgctaaa ttcttcataa gcttcaataa tagtttccag ttgcatccgc tgctgggctt
      961 tatcgccatt gagcaacatc cacaaatcct gaatagctgg accattacgt gcatcatcca
     1021 gatcaacaaa cattggacca tcgcgccaga gaatattccc ggcgtggcag tctccatgta
     1081 gccgcagaac ggtgaaatct tcccgccagt gtgctgtaac ggcggcgatc agctcatctg
     1141 tcgctttcag aaatgccgct ttcaacccgg aaggtatcag tgtagcgtcc tcaaacagct
     1201 tgcgaggctc aatgagatat tcattcagac cgatggtcgg gcgatgaata aaaagctgtt
     1261 tgcgccccgt ctggtgcata cgccctaaat aacgcccaac cgcttccatc tgatcgatat
     1321 tatcagcttc gaactggcga ccaccgacgc ttggaaaaac agcgaaataa aatccctgat
     1381 gattcaataa agtctgaccg ttaaaggcca caggtgctgc gaccggaact tcatcattta
     1441 ccagctgcaa cgcaaattga tgttcttcga ggatttgatc ggctgtccaa cgttcagggc
     1501 gataaaattt gacgacaaaa cgtcgacgct cttcgtcctg aaattgatag acacggtttt
     1561 catagctgtt aagcggggta agaccggaat ccacccggat cccttgctta aacagagcgt
     1621 ccatgatggt atccgggtgt agtgtctgga aagtaaaagc gctgttgttc atcccatcat
     1681 ccggaaaaca ttacgaatga tgaaggatat catttcacga tgattttggt ggcgccgctt
     1741 acaagctttt actctttaat tacgccgcga gcgcgtaaca gcgccgtttt gaagtcttct
     1801 tcataatctt tctgcaaacc cgggatcacc gcatctttcg cggaatcacg cattttcagg
     1861 tgatagatca gaatgtcatc cgtcaaatcc gccagttcgc cgtcaaaacc tgactctttc
     1921 gccagtttct gcaaaaattg caacaagtta aagtctggct ctttttgcca ggctggctgg
     1981 aggagttcaa taacttcatt cagacgttta catttcatag taatgctcct tactcttgag
     2041 acagacacgt tagcagggtc aatcccacaa taaaagaggc gatatcggtg aatctaatga
     2101 cgacgataac aggcgttgtg ctggcaggcg gtaaagccag acgaatgggc ggcgtagata
     2161 aaggattgct ggacttaaat ggcaaaccgc tgtggcaaca tgtcgctgac gcgcttaaga
     2221 cgcagctctc tcacgtcgtg gttaatgcta atcgtcatca ggaaatctat caggcaggcg
     2281 gtctgaaagt gattgaagat tcactggcgg attacccagg ccctctggca ggaatgcttt
     2341 cagtaatgca gcaggaagct ggtgagtggt ttttgttttg tccgtgcgat acgccttaca
     2401 ttccccatga tttagccgcc cggcttaatc atcagcgcaa agatgcgcct gtcgtgtggg
     2461 tccatgacgg tgaacgcgat cacccgacta ttgctctggt aaaccgcgct attgagcctt
     2521 tattactgga atatctgcaa gcaggagaac gccgggtaat ggcatttatg cgtctggctg
     2581 gcggtcatgc ggttgatttc agcgatcata aagatgcatt tgttaacgtg aatacgccag
     2641 aggagcttgc ccgatggcag gaaaaacgat gataccgtta ctcgcctttg ccgcgtggag
     2701 tggtaccgga aaaactacgc ttttgaaaaa actgatcccg gcattatgcg ccagagggat
     2761 ccgtccgggg ctgattaagc atacgcacca tgatatggat gttgataagc caggcaaaga
     2821 tagctatgag ctgcgcaagg ctggcgcggt acaaaccatc gttgccagcc agcaacgatg
     2881 ggccttgatg acggaaacac cagacgaaga agagctggat ctacattttc tcgcaagtcg
     2941 aatggatacc tcaaagctgg atttgattct ggtcgaaggg tttaagcatg aagagatcgc
     3001 aaagattgtg ctgtttcgcg atggagccgg acatcgacct gaagaattag tgatagacag
     3061 gcatgttatt gctgtagcca gtgatgtgcc gcttaatctt gatgtcgcat tactggatat
     3121 taatgatgtt gaggggctgg ctgattttgt ggtggagtgg atgcaaaagc agaacggata
     3181 agcaagagta ggctggatca gacgc
//
LOCUS       DAZPAB010000295         3179 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZPAB010000295 DAZPAB010000000
VERSION     DAZPAB010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.296"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            418..1527
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUPG_003995"
     CDS             418..1527
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUPG_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR0863374.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(1772..1906)
                     /locus_tag="ACHUPG_003996"
     CDS             complement(1772..1906)
                     /locus_tag="ACHUPG_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0863375.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(2552..2722)
                     /locus_tag="ACHUPG_003997"
     CDS             complement(2552..2722)
                     /locus_tag="ACHUPG_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR0863376.1"
                     /translation="MNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHELAEELQNSI
                     QIRFEAESETGT"
BASE COUNT          801 a          659 c          774 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg ggggggaccc tgagcaggtg gtaattgtaa gacgttgcgg
     2821 gcgacggtat tacaattgca catcctgtcc tgtttcttta ggacgtatca tggcacagca
     2881 tatcaactga taaataatcc ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc
     2941 gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga gtctgttctc
     3001 atgcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt ctttataaat
     3061 agagagttat gacaaattag ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc
     3121 ggcgcggtgt tgtagctgcg ccaacaccgc tttttagggg tggtactgac tattttcat
//
LOCUS       DAZPAB010000296         3163 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZPAB010000296 DAZPAB010000000
VERSION     DAZPAB010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.297"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..1224
                     /locus_tag="ACHUPG_003998"
     CDS             <1..1224
                     /locus_tag="ACHUPG_003998"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR0863377.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1224..1958
                     /locus_tag="ACHUPG_003999"
     CDS             1224..1958
                     /locus_tag="ACHUPG_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0863378.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2425..>3163
                     /locus_tag="ACHUPG_004000"
     CDS             2425..>3163
                     /locus_tag="ACHUPG_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR0863379.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRL"
BASE COUNT          858 a          618 c          780 g          907 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgttggg aagagatgcg tcaaggtatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgcttg caaatatagg tgatttttta
     2221 ttgtaggctt ttgaaggcta ttataactgt gcgctaaatt aatgcgttga ataaaagaaa
     2281 taacaaaatt aagaaaagcc atatttacga tatgggcaac ataaatataa tctaaaaaag
     2341 agtaaggcgt tgtaggttgg ttccgcgttc attaccttgt tagtgaacgc ggatttatca
     2401 gttgtcgaaa tggtttttat aataatgaaa aattttaatg tactaaccaa agtcgtattt
     2461 tgcttgtcgt ttttatcaag cgcatttccc acctatgctg cggatgatgt caaagtgact
     2521 caaaacactt cagtttttaa actgaagctt agtgcaacgc gattaatcta tgaacaaggg
     2581 tcggatggcg cagcgataac tgtgacaaat ccgcaggatt ggccgatgct tgtgcagagt
     2641 aaagtctttg aagaagataa agtaactgca gcgcccttta ttgtcactcc gccactgttc
     2701 aggcttaatc caggccagca aaataaaatg tgggttgtgc agactgcgcc agctttacaa
     2761 gacagggaaa aattacactg gctatgcgcc accggcatta cgcctaaaaa gagtgatgtt
     2821 tgggccgaga aagataaaga aaaaattgct gaaaatgtga acctgaacat ccaggttaac
     2881 gtacatacct gcataaaact tattaccagg ccatctggta ttgaaggcaa gatggttaat
     2941 gcgagtgaga agttatcgtg gaaaaagcag ggtgataaaa ttgtcgcggt caatcagtcg
     3001 ccgttctaca tcaacctttc ttctttacag gttggcggta gcaagattga gaaactagat
     3061 tatattgcgc cgttttcgca acatgagttt gttatgccaa aaactagcgg ccaggatatt
     3121 aaatggtctg tgattactga tgaaggtggt gagagtcgtt tgt
//
LOCUS       DAZPAB010000297         3149 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZPAB010000297 DAZPAB010000000
VERSION     DAZPAB010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.298"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            220..552
                     /gene="hdeA"
                     /locus_tag="ACHUPG_004001"
     CDS             220..552
                     /gene="hdeA"
                     /locus_tag="ACHUPG_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR0863380.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            668..994
                     /gene="hdeB"
                     /locus_tag="ACHUPG_004002"
     CDS             668..994
                     /gene="hdeB"
                     /locus_tag="ACHUPG_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR0863381.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            1058..1704
                     /locus_tag="ACHUPG_004003"
                     /pseudo
     CDS             1058..1704
                     /locus_tag="ACHUPG_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(1746..2276)
                     /locus_tag="ACHUPG_004004"
     CDS             complement(1746..2276)
                     /locus_tag="ACHUPG_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR0863382.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(2432..2998)
                     /gene="slp"
                     /locus_tag="ACHUPG_004005"
     CDS             complement(2432..2998)
                     /gene="slp"
                     /locus_tag="ACHUPG_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR0863383.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          924 a          615 c          683 g          927 t
ORIGIN      
        1 gctatcaatc tatgccaaaa acgcgtctaa gaatgcagtc gatttaataa aaatttccta
       61 attgcagtat ctgatgcatc tgtaactcat tgtattgaaa taaaaatatc tgattttgat
      121 attttccatc aacatgacat atacagaaaa ccaggttata acctcagtgt cgaaattgat
      181 tcgtgacggc tctttcactt tatagttgag gatattacga tgaaaaaagt attaggcgtt
      241 attcttggtg gtctgcttct tctgccagtt gtgagcaatg cagcggatgc gcaaaaagca
      301 gctgataaca aaaaaccggt caactcatgg acctgtgaag atttcctggc tgtggacgaa
      361 tccttccagc caactgcagt tggttttgct gaagcgctga acaacaaaga taaaccagaa
      421 gatgcggttt tagatgttca gggtattgca accgtaaccc cagctatcgt tcaggcttgt
      481 actcaggata aacaagccaa ctttaaagat aaagttaaag gcgaatggga caaaattaag
      541 aaagatatgt aattccggga atgcgttaca tcgtacttcc ttgcatattg aacaggccgg
      601 aatatcttct ttaaaagcag ctattcctcc tgttcatata taatctctat attgaatggg
      661 ttacaaaatg aatatttcat ctctccgtaa agcgtttatt tttatgggcg ctgtagcggc
      721 tttgtcactg gtgaacgcac aatctgcgtt ggcagccaat gaatctgcta aagatatgac
      781 ctgccaggaa tttattgatc tgaatccaaa agcaatgacc ccggttgcat ggtggatgct
      841 gcatgaagaa acagtatata aaggtggcga taccgttact ttaaatgaaa ccgatctcac
      901 tcaaattcct aaagtgatcg aatactgtaa gaaaaacccg cagaaaaatt tgtatacctt
      961 caaaaatcaa gcatctaatg acttgccgaa ttaatgaggt acaagtaaaa aggagtagca
     1021 agttgagcca tcttgctgct cctttttgca tttttatatg acagcagaat ttattatacg
     1081 tcttatactc gcggcaattg cctgtggcgc tattggcatg gaaaggcaaa tgcgcggcaa
     1141 aggagcaggg ttacgcacac atgtattaat tggcatggga agcgccctgt ttatgattgt
     1201 ttcgaaatat ggttttgctg acgtgctgtc tttagatcac gtcggactcg accccagccg
     1261 tattgctgct caggtggtga cgggcgtcgg gtttatcggt gcaggtaaca ttctcgttcg
     1321 taaccaaaat attgtaggtc tgacgacggc agcggatatc tgggtgaccg ccgcataggt
     1381 atggttattg gcagcggtat gtacgaactg ggtatttatg gttcagtgat gaccttgttg
     1441 gtgctggaag tcttccatca attaaccttc cgcctgatga acaaaaatta ccatttacag
     1501 ctgacattag tgaacggtaa tacggtatca atgctcgact ggtttaagca gcaaaaaata
     1561 aaaactgatt tagtttcgtt gcaggaaaat gaagatcatg aagtggtcgc cattgatatc
     1621 cagcttcatg ccacaacgtt gatagaagac ctgttgcgat tattaaaagg tatggcaggg
     1681 gtgaaaggcg tttcaattag ctaatctgtg tggcagcgta gccagactca ccgtaagcct
     1741 gaaattcaca ccagataatc aatatgctga tggcgtacca gctcattgat ccgcttaaga
     1801 ttgaggatca tcatgatatt gtgtttatgg cagtagaacg tttttagcga tattttcagt
     1861 ttctccagaa tatcttcagt tgtcatttga ttcgcaatgc agctcagaac ctgatgttgc
     1921 ttgcgcgaaa gtgacacatt cgcttcaggc acagagcagt aactgccatt gatgagtgcg
     1981 acaaaatcaa tgatagtgat agtccgcgga acaaaggtca ccggtacttt ccccagacag
     2041 cgtttaatat taaaggggga gaaaacaatg acatggacag gttttaatcg ttcaatttga
     2101 gtgagaaaat tagaatgaaa tacattattc attaatgtat caataatgac gaaggcattc
     2161 tgatgcaaca ttacgtcgat ctcttcttcg ttctgtatat gaacgacatt acctttactc
     2221 agaatgtttt tcatcgcggt gaagaacatc gtatccctgg taattataag aaacataacc
     2281 gactcctggt gcggacatta actttataat cagtttgcca aaggtaatta acaggacagg
     2341 gcttataata ataatagtta gcttactgta tattaatttt atgtaataca tgatgcagat
     2401 gctgagaaag catctgcatc tttcggtggt gttatttgac cagctcaggt gttacctgac
     2461 tcaccgcatt ggtgtagtaa ggcgcacccc agcccggttc cggccagaat gcaccatagc
     2521 cgtaatccca caggttatag gtggtattaa ccacttctct caaatgccac acctggatgc
     2581 cctgcatatt cacttccagg aagttatacg ggactttatt gataaagcct ggttgttcac
     2641 cctgaatggt accgaggatg gtaacaaagt gattacgata gttcactgga tcaaggaagc
     2701 cgctttgtct ggcgagcagt cggccctgat agttggcttc aatatcaggc ttcgcatagc
     2761 tatccaacgg taatacagcg atttctaaca acgtatccgt tttgccattg ataacgttga
     2821 taaccttccc accaaagcgc gcttgttgac caacatataa ccccggctgg ttatgaacag
     2881 caacaaaact tttttgaata tcaggttggt tattgccttt gatattttgc ggaattgaac
     2941 tacatgcggc aagcaaaaat gaaaggctga ggatgagtgc accttttgtc atgttcatgt
     3001 tactatcctt atcaacaaat attaaaacca taatagatat ttctttatag ttttcatctg
     3061 attctgaggt gatatgcctg atagtaacgt aaacctgtct gatgtttata tcccttctgt
     3121 ttagttcaat tccgttattt tggggcgtg
//
LOCUS       DAZPAB010000298         3112 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZPAB010000298 DAZPAB010000000
VERSION     DAZPAB010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.299"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="ACHUPG_004006"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="ACHUPG_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR0863384.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="ACHUPG_004007"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="ACHUPG_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="ACHUPG_004008"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="ACHUPG_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFR0863385.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="ACHUPG_004009"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="ACHUPG_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFR0863386.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2163..3112)
                     /locus_tag="ACHUPG_004010"
                     /pseudo
     CDS             complement(2163..3112)
                     /locus_tag="ACHUPG_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          773 a          753 c          757 g          829 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc
     2161 actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc
     2221 cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag accattacgt
     2281 tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg
     2341 gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg
     2401 ttgtccaggg gcacagctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga
     2461 atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc ctgctcttca
     2521 agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt
     2581 ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc
     2641 agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct
     2701 gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac
     2761 acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg
     2821 tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca
     2881 ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg ggtatcaagg
     2941 agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc
     3001 agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg
     3061 acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag gg
//
LOCUS       DAZPAB010000299         3097 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZPAB010000299 DAZPAB010000000
VERSION     DAZPAB010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.300"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(104..1813)
                     /locus_tag="ACHUPG_004011"
     CDS             complement(104..1813)
                     /locus_tag="ACHUPG_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFR0863387.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVSGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2245..2964)
                     /locus_tag="ACHUPG_004012"
     CDS             complement(2245..2964)
                     /locus_tag="ACHUPG_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HFR0863388.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          954 a          588 c          518 g         1037 t
ORIGIN      
        1 gccaattgag gtggttgcct gaaagttggc agttacccgg tcactacacc ttgttggagt
       61 gaacatatca aaaacaggta attacacaat ttaaattaca gtctcataaa aaatgattaa
      121 atctatttat tatttcaata aacgtttttt tcatcttctt tgaaaattgt tctgctatag
      181 tatccggttc cagattgttc tcttttatta ttctacaata ctctaatatt gtttctttgt
      241 ctctatttac tacagataca aatacaccag gaataccatc tttattagtt gcggacagca
      301 tttcgaccaa tcgtcttgtg gtaaaactat agttttttag aatattacaa aaggcacgta
      361 ttgtttcggt atggccatta taaaatgcca taaaaagtcc tgatgtaccg ttgggatttt
      421 ttatccataa taattcatct atatattcct ggtgaggggg gagtattttt agtatatctc
      481 catacgccat aatggccttt gtgtgcccat tttgaagtgc cataaacaac ccatgcttta
      541 ctttcaataa ttctttaatt tgttcactac taagcatttt cttattaagc agacttccga
      601 acatacgaat actatctgca tgtccattct gcaatgcaaa aaataaccca ggtaaatcat
      661 ggctgtcttt cgcagctaat agatataatc tcttatcgga agttaacggg gttttaaaga
      721 ctatttcccc ataggctttt attgtgtcta catgtccgtt cataaaagac atgcataaac
      781 ccgacacatt gtttgcccct accgcagcaa gcaatgtaag tattgtttct tgactaagat
      841 tgcacatatt taatatctta ccatatactt gaataatgtc tgaatatccg ttttgaagtg
      901 ctatataaaa tgcaggagta ccatcagagc ttctcgtggc cagaaggttc accagtttag
      961 attgagaaat ttcaccattt ttgtattttt ccatcagtat aaataatact tctgtcagat
     1021 tctttgttat cgcatgatat ataactctgg aattgatcaa ttcatctggt aaaagtttca
     1081 ctgtgtttgt tagaggggaa ttatgcctat cttcaaatat tacctgcccg ggttgctcca
     1141 gaccataaat tttccaataa aaatcatcaa aaaaatcctt agcggataat tgactcagtt
     1201 cgaaatggct atcacaagta aatacagtac gtttatgagt aacagttcga ttaggatcga
     1261 aaaactgaac aacccatttt tcactttctt gtacgcgctt tattttcaac cctaacgcca
     1321 tttggtggtt taatgtgcat gctgataatg ttctgacaaa gtctccattt tttttcatgt
     1381 tataaaaaaa attagtaata actgttcccc actttttatt ttccacaaaa taaatataag
     1441 ctggtgattt tgaaaaatct tccgtttttt ccaaacatac attttttgca atagcatcag
     1501 gggtgctaag aagctgatag tttaccttcc catttgtttc cataaaattc atttcccaat
     1561 aggaagctag gtgacggcaa actatttgtc ttccatatat atcttttgcc actccattta
     1621 tatttacctc cacatttctc ttttctgaaa aataaggtgc tgttttattc ttaaactgaa
     1681 ttagttcact ggagtttgag accctcataa tagaacttgt tattgttcta ttatctctgt
     1741 taacagacat atctgttgat aacgaatttt ttgttgaaaa tatactagat gaaaatgttt
     1801 tatttaacgg catagaagac aaccatagag atgttataat actattagat tgcagttact
     1861 aatagtaaaa gcctccttac aaggaggtag cttattcaga atgctcctat aagatagcga
     1921 gctcgtcaac gatacagatg atctgattat attgtgcaaa aggagaacag gaaaataggc
     1981 actggaaaag atgaaggcct gatgggagca ttaactaatg caaaatccga caggttaata
     2041 tcccgaaaag caatcaaacg actcaaacag gagatgcacg ctgaaatggt ggacaactgt
     2101 tcggatttca gatgagtagc tcagcagttt atgagccgaa tgctctgtca cgggcaggaa
     2161 tcaggctgca tacgcagtgc caaatctaat cgtagttatt ctaaccagtt ttcaagctac
     2221 aaggtggtgt agctggcatc ttctctactc tatcatcaaa cgataaaatg gttcctctcg
     2281 taacatttgc tcttgccttt cacttccatc tcgatcgctt cgtagttcat tacgatagct
     2341 gacatatttc cagtcttcag gtctaacatc tgacgccgga tactccccgg gagcgatttt
     2401 gcttcttaat agattagact cgagacacat tataaattgt cgtattttat gaagtaataa
     2461 agcactatat tgcgagcatt cctgatctga ttttacatat agcgtaaact gagctcctat
     2521 ccccacacga gattgctgag aaactcgatt catatcagtt atcttccatt tatctatagg
     2581 actgtcctct gaaaataata aaccagacaa aatttggaat gccagaggaa cttgctccct
     2641 agctatacta atatgaaact tgtccccaac aaaatccccc ttactctgag gattttcacg
     2701 acatgcatga ataaacacat ctccactatg gttcacaatc tggaatcctt ggtacatgac
     2761 agaaaaagat tgacatatat cagagtaagt tggcacgttc gatgctactt tgaaatagta
     2821 cgctgggtat tgccgtattt ggttgtacaa tatattgaag ttatttttca atctttcatt
     2881 cgcagaaagc atctgcggta tttcagacct cacaacattc aaagaatcta aatttagttt
     2941 tagacagggc ttttttatgg gcatagaaaa cgtcctctat aaaatagata tattatctta
     3001 tattgattgt ttatcatctt ttataaaacg atacattata tgtaatgtat gtaagcgtaa
     3061 gggtcctctc cgaacatggt gggaaggctt agaacgc
//
LOCUS       DAZPAB010000300         3069 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZPAB010000300 DAZPAB010000000
VERSION     DAZPAB010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.301"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..498
                     /locus_tag="ACHUPG_004013"
     CDS             <1..498
                     /locus_tag="ACHUPG_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0863389.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            578..1273
                     /locus_tag="ACHUPG_004014"
     CDS             578..1273
                     /locus_tag="ACHUPG_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0863390.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1302..>3069
                     /locus_tag="ACHUPG_004015"
     CDS             1302..>3069
                     /locus_tag="ACHUPG_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0863391.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          888 a          718 c          684 g          779 t
ORIGIN      
        1 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
       61 gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt
      121 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      181 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc
      241 cctaatctgg caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt
      301 attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg
      361 atttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac
      421 gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta
      481 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      541 cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt
      601 gatattaacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      661 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      721 caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag
      781 ccgtgcccca tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc
      841 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      901 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      961 gctggtattt aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
     1021 aaacgatgcg tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa
     1081 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
     1141 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
     1201 aaagaacgtg aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg
     1261 tccactgcaa taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc
     1321 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg
     1381 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1441 ccattatgct ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1501 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1561 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1621 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1681 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat
     1741 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1801 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1861 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1921 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
     1981 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     2041 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     2101 gctacgcctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     2161 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     2221 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     2281 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac
     2341 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2401 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2461 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2521 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2581 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2641 ttcatggcta tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata
     2701 tcgctgttag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2761 cccgcaattg gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2821 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2881 acgagaaaaa cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     2941 gcctgaatta tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga
     3001 atctcagtat tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat
     3061 cacttaacc
//
LOCUS       DAZPAB010000301         3063 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZPAB010000301 DAZPAB010000000
VERSION     DAZPAB010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.302"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACHUPG_004016"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="ACHUPG_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR0863392.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKSSSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="ACHUPG_004017"
     tRNA            complement(621..695)
                     /locus_tag="ACHUPG_004017"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="ACHUPG_004018"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="ACHUPG_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="ACHUPG_004019"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="ACHUPG_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR0863393.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          754 a          725 c          718 g          866 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagacttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2881 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc
     2941 atcaaaatta acacctagtg cagatagttg attaatttca ttttgatatt tcgtctggcg
     3001 attttgaagt cttttttcaa ctttatcttc gagattctta aagtctttgt atttattttc
     3061 agg
//
LOCUS       DAZPAB010000302         3048 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZPAB010000302 DAZPAB010000000
VERSION     DAZPAB010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.303"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..955)
                     /gene="mxiG"
                     /locus_tag="ACHUPG_004020"
     CDS             complement(<1..955)
                     /gene="mxiG"
                     /locus_tag="ACHUPG_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HFR0863394.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELIN"
     gene            complement(964..1422)
                     /gene="ipgF"
                     /locus_tag="ACHUPG_004021"
     CDS             complement(964..1422)
                     /gene="ipgF"
                     /locus_tag="ACHUPG_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HFR0863395.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            complement(1422..1784)
                     /gene="ipgE"
                     /locus_tag="ACHUPG_004022"
     CDS             complement(1422..1784)
                     /gene="ipgE"
                     /locus_tag="ACHUPG_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HFR0863396.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            complement(1794..>3048)
                     /locus_tag="ACHUPG_004023"
     CDS             complement(1794..>3048)
                     /locus_tag="ACHUPG_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HFR0863397.1"
                     /translation="IRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISHHGKNY
                     GFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKEIFSGI
                     RHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKIVSTSL
                     LTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVVTFNFG
                     VNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKNDVIYL
                     ANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQDAEIKR
                     EIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKKLPLSS
                     LELSYSERIGDPKIWNMVKGYSSFV"
BASE COUNT          897 a          666 c          382 g         1103 t
ORIGIN      
        1 tattaatgag ctcaagcttt tcatctgtca gttcttctct gacagaatat gtaactttat
       61 tattttcaca aatctcttta tactggacat ttacctttgt gaaaatacct ttagcatccc
      121 cccttgcatt atgatcgcta agatagttga atttaatatt tttgatatac ggaaattcaa
      181 caatcagagc atttttcaat ttatcaagtt cggtatcttt tgaactattt ctttctttac
      241 ttaaaaacac cagtaattcc gctttatcac tcactaaatt caacacatac aaattgataa
      301 atgggaatcg ttgattgata tattgttgaa ttctatttat ttctttgttt gacaccagaa
      361 tatatctgca tttggtaaat ccggttttat ttaaggctac agacgcccat accgcatcat
      421 tttgtgtttc ggcaagaata taattgcact gtccagtgtt gactattcca tatctcttat
      481 catctataat ttcagcaatt tcttttactt catctttctt taaagagagg gaaaaaatta
      541 taattaacac aacaataaca gcaaagaaaa aaaacaccga gtggttctta tacattccgt
      601 ttagtatatg gcctctcgat ttatcctccc tcatattttt aaggtgaaaa gaaatcccgt
      661 catattcaaa tgaattaaat tgtgaattga tatcaatttg ctcacctttt tcatcactaa
      721 attgacagat attatctttg cttatggtca aataaattat accatccgat ttactatctg
      781 ttacaggaat aatattttct gggaaattgt cgtttccaaa ctcaagtgtt tctatcgtcc
      841 gtccgagaac aattaagtta ttcccataat ataacggaaa ctcatccccg acaccgtttg
      901 tgagtttcac taataacctg aatggtgcaa gatttgagtt ctttgcctca gacataaatc
      961 ctcttatatc cttcgattat tctgcttgct ctctgctgct atccgcaaat agcgtctata
     1021 gatatcctct gcgtacttta atctttcttt tttctttttc ggagacgttc ctgcattata
     1081 ggctccaaca gcctcccacc ctcttccata catcatcata aactcattta ataactttgc
     1141 tgcataatgt acagaaagac atggatgact aattagcatt tcctcagaat atcccatttc
     1201 tctaagtctt ttagaatgaa agtcatttat ttgcattata ccataatctt tacttccatt
     1261 gttattaaca ttgactgcag atttattaaa tccggattct ttttccgcaa tcgcttttaa
     1321 caaacttgat ggaatattgt acctttcacc agccttatcc caacaatcag ctctccctaa
     1381 atatgggata aaacacagca aaataaatac gaaacgggac attaataccc cttcattctt
     1441 cgcgcaaatt catccttcag ccaacgcaca cgcgaaatat aatattctgt attaacatat
     1501 acatcctcga attcatttat cccggtaaga tctaatcgcg caattagatt tccgccttca
     1561 tcatcagtag ctaagcatat cttttctgag taatttaggc tcaacgcata tataagatca
     1621 tttatatttt caggcaatgc acaaaaaggg cataataaat ttattgaatc tccttctttt
     1681 tcaatatata ttagcacatc atcatcaagc atgattgctt gatcatcgat atcaattaaa
     1741 gggataccca aggctcggca aataacatct gctaaatctt ccatatattc ctcttataca
     1801 aatgacgaat accctttcac catattccat atttttgggt cccctattct ttcagaataa
     1861 gatagctcta acgaagatag cggcaatttt ttcatgactt tatttccggg cacaccagta
     1921 ttcatctctt ggatttccat attaccacta ttcattagaa tagtagaaaa taatcttttc
     1981 tcttctgagg ataatttact atttaattga gaaaactgac ctgtttcatg ttttcttatt
     2041 atttctcttt taatttcagc atcctgcata cctgttctgt ccttcccact cttacaattc
     2101 cagcaaggta cagcacctat agtataagcc aatagagtca ttctttgtga caatttatac
     2161 ggctctccat tatcattttt ttgtaacttt ttggttacaa tctcttttat ctggttagcc
     2221 agatatatca catcattctt acatggtgga tttttttcta ttgcttctgc agcccaacct
     2281 ccaatcacac catttttgag gaaattatcc cccaacaaag aacatattga ttcatcatta
     2341 agcttgtcaa cattcctcca gcctaagccc atttttagcg ctaattcatt tacaccaaag
     2401 ttaaatgtca ccacttttag attaacgctt acttctttca gaagaccatc actattccga
     2461 attaataatt tcgttggctt ccccctttta gagtttaacc cttttaatgc gttcacctga
     2521 tcttttaaca tactctcctc tcccccggtt aaactggtcg gcgtcagaag agaagtcgaa
     2581 acaatcttta aatctactgt tttaccagac aaagcctgtg ataataactc tggtctgcta
     2641 tataatgctg cgcttactaa ctcctcagct ttattacgag cagcaacagc tctttcagat
     2701 gagttttttt tcaatccata ggctgatata actccatgtc tgatacccga aaaaatttcc
     2761 ttcccctcat catccaccac ttttgaaagc cacatattcg cgatatgatc actctctctc
     2821 gtagaagcac aacaaattcc ttttccatta tactctttga caaatatatt tttattccct
     2881 atcttcatat gagaggcagg agttaattga aaaccataat ttttcccatg atgactaata
     2941 ttcttgtcga ccggtcccca attcttatta tttaaataat cagtaaatat ttgttttatt
     3001 ttcgtttgca attccttttt atttttaatg cctagctgtg ctcttatc
//
LOCUS       DAZPAB010000303         2974 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZPAB010000303 DAZPAB010000000
VERSION     DAZPAB010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.305"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..2850
                     /locus_tag="ACHUPG_004024"
     rRNA            <1..2850
                     /locus_tag="ACHUPG_004024"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          786 a          653 c          927 g          608 t
ORIGIN      
        1 gacgtgctaa tctgcgataa gcgtcggtaa ggtgatatga accgttataa ccggcgattt
       61 ccgaatgggg aaacccagtg tgattcgtca cactatcatt aactgaatcc ataggttaat
      121 gaggcgaacc gggggaactg aaacatctaa gtaccccgag gaaaagaaat caaccgagat
      181 tcccccagta gcggcgagcg aacggggagc agcccagagc ctgaatcagt gtgtgtgtta
      241 gtggaagcgt ctggaaaggc gtgcgataca gggtgacagc cccgtacaca aaaatgcaca
      301 tactgtgagc tcgatgagta gggcgggaca cgtggtatcc tgtctgaata tggggggacc
      361 atcctccaag gctaaatact cctgactgac cgatagtgaa ccagtaccgt gagggaaagg
      421 cgaaaagaac cccggcgagg ggagtgaaaa agaacctgaa accgtgtacg tacaagcagt
      481 gggagcacgc ttaggcgtgt gactgcgtac cttttgtata atgggtcagc gacttatatt
      541 ctgtagcaag gttaaccgaa taggggagcc gaagggaaac cgagtcttaa ctgggcgtta
      601 agttgcaggg tatagacccg aaacccggtg atctagccat gggcaggttg aaggttgggt
      661 aacactaact ggaggaccga accgactaat gttgaaaaat tagcggatga cttgtggctg
      721 ggggtgaaag gccaatcaaa ccgggagata gctggttctc cccgaaagct atttaggtag
      781 cgcctcgtga attcatctcc gggggtagag cactgtttcg gcaagggggt catcccgact
      841 taccaacccg atgcaaactg cgaataccgg agaatgttat cacgggagac acacggcggg
      901 tgctaacgtc cgtcgtgaag agggaaacaa cccagaccgc cagctaaggt cccaaagtca
      961 tggttaagtg ggaaacgatg tgggaaggcc cagacagcca ggatgttggc ttagaagcag
     1021 ccatcattta aagaaagcgt aatagctcac tggtcgagtc ggcctgcgcg gaagatgtaa
     1081 cggggctaaa ccatgcaccg aagctgcggc agcgacgctt atgcgttgtt gggtagggga
     1141 gcgttctgta agcctgtgaa ggtgtgctgt gaggtatgct ggaggtatca gaagtgcgaa
     1201 tgctgacata agtaacgata aagcgggtga aaagcccgct cgccggaaga ccaagggttc
     1261 ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa ggcgaggccg aaaggcgtag
     1321 tcgatgggaa acaggttaat attcctgtac ttggtgttac tgcgaagggg ggacggagaa
     1381 ggctatgttg gccgggcgac ggttgtcccg gtttaagcgt gtaggctggt tttccaggca
     1441 aatccggaaa atcaaggccg aggcgtgatg acgaggcact acggtgctga agcaacaaat
     1501 gccctgcttc caggaaaagc ctctaagcat caggtaacat caaatcgtac cccaaaccga
     1561 cacaggtggt caggtagaga ataccaaggc gcttgagaga actcgggtga aggaactagg
     1621 caaaatggtg ccgtaacttc gggagaaggc acgctgatac gtaggtgaag cgacttgctc
     1681 gtggagctga aatcagtcga agataccagc tggctgcaac tgtttattaa aaacacagca
     1741 ctgtgcaaac acgaaagtgg acgtatacgg tgtgacgcct gcccggtgcc ggaaggttaa
     1801 ttgatggggt tagcgcaagc gaagctcttg atcgaagccc cggtaaacgg cggccgtaac
     1861 tataacggtc ctaaggtagc gaaattcctt gtcgggtaag ttccgacctg cacgaatggc
     1921 gtaatgatgg ccaggctgtc tccacccgag actcagtgaa attgaactcg ctgtgaagat
     1981 gcagtgtacc cgcggcaaga cggaaagacc ccgtgaacct ttactatagc ttgacactga
     2041 acattgagcc ttgatgtgta ggataggtgg gaggctttga agtgtggacg ccagtctgca
     2101 tggagccgac cttgaaatac caccctttaa tgtttgatgt tctaacgtgg acccgtgatc
     2161 cgggttgcgg acagtgtctg gtgggtagtt tgactggggc ggtctcctcc taaagagtaa
     2221 cggaggagca cgaaggttgg ctaatcctgg tcggacatca ggaggttagt gcaatggcat
     2281 aagccagctt gactgcgagc gtgacggcgc gagcaggtgc gaaagcaggt catagtgatc
     2341 cggtggttct gaatggaagg gccatcgctc aacggataaa aggtactccg gggataacag
     2401 gctgataccg cccaagagtt catatcgacg gcggtgtttg gcacctcgat gtcggctcat
     2461 cacatcctgg ggctgaagta ggtcccaagg gtatggctgt tcgccattta aagtggtacg
     2521 cgagctgggt ttagaacgtc gtgagacagt tcggtcccta tctgccgtgg gcgctggaga
     2581 actgaggggg gctgctccta gtacgagagg accggagtgg acgcatcact ggtgttcggg
     2641 ttgtcatgcc aatggcactg cccggtagct aaatgcggaa gagataagtg ctgaaagcat
     2701 ctaagcacga aacttgcccc gagatgagtt ctccctgacc ctttaagggt cctgaaggaa
     2761 cgttgaagac gacgacgttg ataggccggg tgtgtaagcg cagcgatgcg ttgagctaac
     2821 cggtactaat gaaccgtgag gcttaacctt acaacgccga agatgttttg gcggattgag
     2881 agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga
     2941 attagcctgg cggccgtagc gcggtggtcc cacc
//
LOCUS       DAZPAB010000304         2951 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZPAB010000304 DAZPAB010000000
VERSION     DAZPAB010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.306"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="ACHUPG_004025"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="ACHUPG_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR0863398.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="ACHUPG_004026"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="ACHUPG_004026"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR0863399.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="ACHUPG_004027"
     CDS             2603..>2951
                     /locus_tag="ACHUPG_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0863400.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          701 c          779 g          813 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttctctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DAZPAB010000305         2932 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZPAB010000305 DAZPAB010000000
VERSION     DAZPAB010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.307"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="ACHUPG_004028"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="ACHUPG_004028"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR0863401.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /gene="ssuA"
                     /locus_tag="ACHUPG_004029"
     CDS             643..1602
                     /gene="ssuA"
                     /locus_tag="ACHUPG_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR0863402.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..2744
                     /gene="ssuD"
                     /locus_tag="ACHUPG_004030"
     CDS             1599..2744
                     /gene="ssuD"
                     /locus_tag="ACHUPG_004030"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFR0863403.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2756..>2932
                     /locus_tag="ACHUPG_004031"
     CDS             2756..>2932
                     /locus_tag="ACHUPG_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863404.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELW"
BASE COUNT          659 a          800 c          806 g          667 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacgcctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg ggggaaaccg
     2041 tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc
     2101 aacagcctta tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg
     2161 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc ggaacttgtt aaagagaaaa
     2221 tcgaacaagt gcgggcgaaa gctgccgcgt atgggcgcaa aattcgtttc ggtattcgtc
     2281 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2341 cgcatcttga tgatgagact atcgccaaag cacaagccgc attcgcccgg acggattccg
     2401 taggacaaca gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc
     2461 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2521 atggtcctac ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2581 tgctttcggg ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac
     2641 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2701 cggtggcaaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc
     2761 aacgccagtg aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt
     2821 ggcggtgtgg caactggcct cctcggttgg ctggctgtcg acgcgtattt tgccttcacc
     2881 ggaaggagtg gtgacggcgt tctggacgct ctccgccagc ggcgaactgt gg
//
LOCUS       DAZPAB010000306         2906 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZPAB010000306 DAZPAB010000000
VERSION     DAZPAB010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.308"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..1541
                     /locus_tag="ACHUPG_004032"
     CDS             <1..1541
                     /locus_tag="ACHUPG_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR0863405.1"
                     /translation="PTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVD
                     TYKIGIDKAIEAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGV
                     YSNTDAQDGIPLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFT
                     TFSAEEWQPQNRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVV
                     DRAKTLDQPFMLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKR
                     ILEQLKKNGQYDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKG
                     KLQPGNYDKLISAMDFYPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWI
                     TSYSHWFDEENIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVEN
                     NQLGLYKLTDLQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKAL
                     SEAK"
     gene            1593..2750
                     /locus_tag="ACHUPG_004033"
     CDS             1593..2750
                     /locus_tag="ACHUPG_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR0863406.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2716..2838)
                     /locus_tag="ACHUPG_004034"
     CDS             complement(2716..2838)
                     /locus_tag="ACHUPG_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863407.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT          883 a          632 c          586 g          805 t
ORIGIN      
        1 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
       61 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      121 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      181 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      241 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      301 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      361 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      421 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      481 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      541 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      601 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      661 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      721 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      781 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      841 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
      901 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
      961 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1021 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1081 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1141 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1201 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1261 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1321 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1381 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1441 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1501 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1561 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1621 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1681 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1741 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1801 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaata
     1861 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     1921 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     1981 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2041 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2101 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2161 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2221 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2281 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2341 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2401 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2461 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2521 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2581 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2641 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2701 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2761 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2821 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     2881 tcagggcatt agcaataccc ttgatt
//
LOCUS       DAZPAB010000307         2894 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZPAB010000307 DAZPAB010000000
VERSION     DAZPAB010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.309"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..308)
                     /locus_tag="ACHUPG_004035"
     CDS             complement(<1..308)
                     /locus_tag="ACHUPG_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR0863408.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     "
     gene            complement(355..1074)
                     /gene="btsR"
                     /locus_tag="ACHUPG_004036"
                     /gene_synonym="yehT"
     CDS             complement(355..1074)
                     /gene="btsR"
                     /locus_tag="ACHUPG_004036"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR0863409.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="ACHUPG_004037"
     CDS             complement(1071..2756)
                     /gene="btsS"
                     /locus_tag="ACHUPG_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR0863410.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          748 a          774 c          657 g          715 t
ORIGIN      
        1 tcatcggttt tcagcgaaaa cgcgatgcgt aattttttca gcacgatgtt gttattaatg
       61 cgacgttccg gctccagtgc cggagcagac tcatccttgc cgcgtttttc ataaatcagg
      121 ccattgagga atgacgacaa aacaatgtcc ggacaacgct gaaaaccctc ttcgtcttct
      181 ttacgtagcc agacggcgat ctgttccgcg gtggcttcga cattatccag cgccagaata
      241 cgcaccaggt cattattatt ggctttcaaa atgtagcgca cgctgcgcag aatatcgtta
      301 cttatcatga ggccttcagg tgttgatgag gcaaaaagcc attttagcag tcttttacag
      361 gccaatcgcc tcttttaagc ttttcagata acggcggctg accggcacgg ttaagccatt
      421 acgcaaaatc aactcggcct ggccgttatc ttccagacga atctcctgta aatgcgcgag
      481 gttaaccaga tactgacgat ggcagcgcag tagtggtgta cgactttcca aggtacgtaa
      541 tgtcaattcg gtaaagccct ctttcccttc gtggctggta acgtagacac cgctcatccg
      601 actgctgaca aatgccacat ctttcatttg cagcaaataa atccgactat gccccgtaca
      661 agggataaat ttcagcgcct gttgattttc cggtaacagc gaaacatcct gcttgctgcg
      721 ctcctgacgc aatcgcgcca gcgttttctc cagtcgcgct tcatcaattg gcttcagcag
      781 ataatcaaag gcatgttctt caaaggcttt aatagcgtat tcgtcaaacg cagtgagaaa
      841 aacaatatac gggcgatgtt ccgggtcaag catccccacc atttccagac cactgatgcg
      901 cggcatctgg atatcgagaa acagcacatc cgggcgcagt ttatgcaccg cgccgatccc
      961 ttccacggcg tttgaacact ctccaacgat ttcaatatcg ctctgctcct gcaaaaatat
     1021 acgcaggttc tcccgtgcta acggttcatc atcgacaatt aagactttaa tcatgcctcg
     1081 tccctccatg gtagtcgtaa cgttattcgg gtgtaactat caggctcaca ggcgacgctt
     1141 attccatagt catcgccaaa ccgttcacgt aaacgcttat ccaccagatt catccccagc
     1201 ccactggcat tggttaccgg ttgatacaaa ccggcattgt cttcgatctc cagcatcaaa
     1261 tgttgcccct cacgtcgggc gctgattgcc actcgccctg tatccagcag ttgtgatgtc
     1321 ccatgtttaa tggcgttttc cactatcggt tgcagggtaa acgcgggcaa ttgctgctgg
     1381 gataattctt gcggaatagc aatgttgacc tgcaaccgcg actggaagcg cgccttttca
     1441 atttgcagat aagcgttcac atgttcaatt tcgtcggcga gagtaacaaa ctccgaaggc
     1501 cgctttaagt ttttgcggaa aaaagtggaa agatactgca ccagctggct ggcctgttcg
     1561 ctgtcgcggc ggatcaccgc tttaatggtg ttaagcgcat taaacaaaaa atgaggattc
     1621 acctgggcgt gaagcagttt gatttctgac tgggtgagca tcgctttttg ccgctcatat
     1681 tgccctgcaa gaatctgcgc cgaaagcagt tgcgcaatcc cctcgcccag cgtgcggttt
     1741 attgaactga ataaccggtt tttggcttca tacaatttga tggtgcccat cacgcgctga
     1801 ttttcgccac gcaacggaat taccagcgtc gaccccagtt tgcattgcgg atgcaaagag
     1861 caacgataag gtacttcgtt gccatcagcg tagaccactt caccggtttc aatcgctttt
     1921 aaggtgtaag tcgaagaaat cggtttgccg ggtaaatggt ggtcgtcacc aattccggta
     1981 aaggccagca atttctctcg atcggtaatc gcgactgcac caatatccag ctcctgatac
     2041 agtacctgag ccactttcat gctgttcact tcgttaaacc cctgtcgcaa aatgccttcc
     2101 gtcgaggctg ccactttcag cgcagtggca gaaaaagccg aagtgtattt ttcaaacatc
     2161 gcgcgtttat cgagcaatat acgcataaac agcgccgcgc cgacggtatt ggtgaccatc
     2221 attggcgcag caatattact caccagacgc accgcatctt cataaggtcg ggcgatcgca
     2281 aggatgatca gcatttgcac catttcagcg acgaacgtga cggcaccggc ggtaatgggg
     2341 ttaaagactt tatcagtgcg cccgcgacgg atcaggatgc tgtgtaccag gccgccgagc
     2401 aatccttcaa cgatggttga gatcatgcaa cttagcgcgg tcatgccccc catcgaatat
     2461 cgatgtaagc cgccggtcag accaaccagc ccaccgacga ccggaccgcc gagtaagccg
     2521 cccattaccg cgcctatcgc acgggtattg gcaatagaat cgtcaatgtg caacccaaac
     2581 caggtgccca tgatgcagaa gatggaaaag acgatgtagc agagaaattt atgcggcaga
     2641 cgaaccgtga cctgcattaa cggtatgaat aatggcgttt tactcattaa ccacgcaatg
     2701 actaaaaaaa cgcacatctg ctgaagcagc agcaacacca gattaaaatc gtacataccc
     2761 gcaaaccaca cttcccttta aaacgcgtaa catacattgc ctgcgtttaa ctttctttga
     2821 actcttgcag aaaaatgaga attcgtgagt acgatcactc aaaatcgcct ggcaaaaata
     2881 aaatcaccct atag
//
LOCUS       DAZPAB010000308         2889 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZPAB010000308 DAZPAB010000000
VERSION     DAZPAB010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.310"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..779
                     /gene="lsrF"
                     /locus_tag="ACHUPG_004038"
     CDS             <1..779
                     /gene="lsrF"
                     /locus_tag="ACHUPG_004038"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFR0863411.1"
                     /translation="WGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIAP
                     LFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNS
                     CAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRI
                     AAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVD
                     MGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            803..1093
                     /gene="lsrG"
                     /locus_tag="ACHUPG_004039"
     CDS             803..1093
                     /gene="lsrG"
                     /locus_tag="ACHUPG_004039"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFR0863412.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1133..1891
                     /gene="tam"
                     /locus_tag="ACHUPG_004040"
     CDS             1133..1891
                     /gene="tam"
                     /locus_tag="ACHUPG_004040"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFR0863413.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1895..2809)
                     /locus_tag="ACHUPG_004041"
     CDS             complement(1895..2809)
                     /locus_tag="ACHUPG_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFR0863414.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          760 a          667 c          771 g          691 t
ORIGIN      
        1 attggggaat gcagtcacgc ttatcgtgga tatttaatcc gaaaacgggt aaaactgtga
       61 tgctggcctt tgaccatggt tattttcagg ggccgactat cggacttgaa cgcattgata
      121 taaatatcgc cccgctgttt gaacatgccg atgtattaat gtgtacgcgc ggcattttgc
      181 gcagcgtagt tcccccggcg accaataagc cggtggtact gcgggcgtca ggtgcgaact
      241 ctattctggc ggaattaagt aatgaagccg tggcgttatc gatggatgat gccgtgcgcc
      301 tgaacagttg cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa catcagtcga
      361 tcaaaaatat tattcagctg gtcgatgccg gaatgaaagt gggaatgccg accatggccg
      421 tgactggcgt gggcaaagat atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa
      481 tcgccgctga aatgggggcg caaattatca aaacctatta tgtcgaaaaa ggttttgaac
      541 ggattgttgc cggatgtccg gtacccattg ttattgctgg cggtaaaaaa ttaccggagc
      601 gcgagacgct ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt gtggatatgg
      661 ggcgtaatat tttccagtct gaccatccgg tggcgatgat gaaagccgta caggcggtgg
      721 ttcaccataa cgaaacggct gatcgggcat atgaactcta tctgagtgaa aaacagtaac
      781 tgcggattta aggagaagaa ttatgcacgt cacactggtt gaaattaacg ttcatgaaga
      841 caaggttgac gagtttatcg aagtttttcg ccagaaccac ctgggctctg tacaggaaga
      901 aggtaatttg cgcttcgatg tcttacagga cccggaagtg aattcgcgct tttatattta
      961 cgaagcctat aaagatgaag acacagtggc attccataaa accacgcctc actacaaaac
     1021 ctgtgtcgcg aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg
     1081 gttgatgccg tgaggctaca attggggtaa cgcgctgacg ggagtaaaaa aaatgtctga
     1141 ctggaacccc tctttatatc tgcacttttc cgctgaacga tcgcgtccgg cggtggagct
     1201 gcttgccaga gtgccgctgg agaatgtcga atatgttgcc gatcttggct gtggcccagg
     1261 taacagcacc gccctgctgc atcaacgttg gcctgcggcc aggataacag gcatcgactc
     1321 gtctccggcg atgattgctg aagcgcgcag tgctttgcca gactgccagt tcgtggaagc
     1381 agacatccgc aactggcaac cggaacaggc actcgatctg atttttgcta atgcctcact
     1441 gcaatggctg cccgaccact acgaattgtt tcctcacttg gtttctttac ttaatccgca
     1501 gggcgtgctg gcagtacaga tgccagataa ctggctggag ccgacccatg tgctcatgcg
     1561 cgaagttgct tgggaacaaa actacccgga tcgcgggcgt gagccgttgg ctggcgttca
     1621 tgcttactac gatattttga gcgaagccgg atgtgaggtc gatatctggc gaaccactta
     1681 ctatcaccag atgccgtcgc accaggcgat tatcgattgg gtgaccgcca ctggattacg
     1741 tccgtggtta caggatctga ccgagagcga acagcagctt tttcttacgc gctaccatca
     1801 gatgctggaa gagcagtatc cactgcaaga gaacggacag atactgctgg catttccgcg
     1861 tctgtttatt gttgcccgcc gtacggagta attatcacgt cagctggtaa tgacgatcgg
     1921 gaagaatttt tgctggaatt tctgcttcat cgttcatctg taacaggtca atttcaatag
     1981 cgttgcagat ggcatccagc ggtaaatcat tgttttcagt accgaacgga tcttccagtt
     2041 cttccgccag acaatccagc gaaataaaag tgtaggaaat cagcacagag ataaaaggcg
     2101 tcatgtaatg caggtccacg accagcgcga acggcagcat gatacaaaac agataaacgg
     2161 tacgatgcaa aatcagggtg taggcaaagg gaattggcgt gtaggcaatg cgctcacagc
     2221 ctgccagaac cgctgaaata tcattaagcc gatcgttgag gctaataaac aggatatctg
     2281 aaagctgtcc attgcggcgc tgaaccgcca accattctcc cattattaac aagatacggt
     2341 tagctggaga gttcgaagcc agtacacgct gaagatctcc agtcttgaga taatgagcca
     2401 gcacttccgc ctgtggctgt ttgcgtaatg tcatgcgtaa acagtgggcg aaggcgattt
     2461 gcagcctggc aaactccctt acacttgccg aatccggcaa tgtcgttttt acctctcgca
     2521 gtaacgaccg tgaggcaatc atcaactgcc cccaaagttt tcgcgcttca acgtaacggg
     2581 cgtacccggc attattacga aaaccaagaa aaatggcgat ggcgacaccg agaatgctga
     2641 acggtgcgag ggtgaatttg atgcccagat gcgtgtacca gggcagcatg aatataacag
     2701 caatagaaaa gagaaaattg agtagtaagc gcgaggatat cttggataat actgagccgt
     2761 gccagacaaa aatacggcgc agccagtgtt gttgtggacg aacaatcatg gttatcttca
     2821 ggcgtggaaa agtcgcccta ttaaacgtga ttacgatcac attctcaaga cgctacttac
     2881 aaattacct
//
LOCUS       DAZPAB010000309         2862 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZPAB010000309 DAZPAB010000000
VERSION     DAZPAB010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.311"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..647)
                     /gene="yidZ"
                     /locus_tag="ACHUPG_004042"
     CDS             complement(<1..647)
                     /gene="yidZ"
                     /locus_tag="ACHUPG_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR0863415.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
     gene            complement(622..1797)
                     /gene="mdtL"
                     /locus_tag="ACHUPG_004043"
     CDS             complement(622..1797)
                     /gene="mdtL"
                     /locus_tag="ACHUPG_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR0863416.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(1929..>2862)
                     /gene="tnaB"
                     /locus_tag="ACHUPG_004044"
     CDS             complement(1929..>2862)
                     /gene="tnaB"
                     /locus_tag="ACHUPG_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFR0863417.1"
                     /translation="SANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAAS
                     RITSLFLGLKIISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFG
                     FHGNIPSLIICYGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGG
                     NIDSLVKSFLGTKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGR
                     FKTVLLTFLPPALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFT
                     VWGGNLIPAIVILFGITVILCWFGNVFNVLPKFG"
BASE COUNT          833 a          745 c          727 g          557 t
ORIGIN      
        1 caggtgtcgc tctgttccca gcaaatgctg atatgcggat aacgtaaaaa ggtgtccaga
       61 ttccacgttt catgcagtgc cggatgatct ttgcgtaacc agacgcaggg tacatcacta
      121 aacagcactt cataatcaat ggctaacggt agcgagctta acagctcccg cgagcgagga
      181 tggctttcgc gaccggaaaa accaatatcc acttcaccac gagtaatggc atctaaggaa
      241 tcgtaatccc agttacgtag tttgatggtc gcctgcgggt aacgttggta gatccgtttc
      301 gacagcgcat taagcatgat catcatcagc ggcgattccg ccgccaactc aaacttcagg
      361 ccgcgcggtg tctggtggtg cggtttatcc agcagcagat tgctcatttg catccattcc
      421 gccagatttt gctccatgct gaccattagc tgtgtgggcg acagccccag cggtgagttc
      481 acaaagagcg ggtcgtcaaa ccacgctctt aactttgcca gcgacttact caccgctgaa
      541 ggggtcacgt tcatccgctt cgccgctttg gtcacgctgc gctcctgcat cagcagttgc
      601 agacagagca gcagattgag atcaagcgtg gtgatggatt tcttcatgag cggcaacggg
      661 gcgtccaggc gcgacgaaca taatcagcaa caggctcact atgctacagg caatcagaat
      721 cccgatcagc atattccatg cgccgatacc aaccaccgcg gccagccaaa tccacagtga
      781 cgaaccgcaa acctgcgcaa tacctaaggt cgagctggct acgcccgcgc gtaatgaaaa
      841 tggtcctaac gcctgactca tcgctacgcc aaaacctacc gagaaaccgg cgcaaatcag
      901 cgtgatacca aacagagaaa ccgcatggga aggtgaaacg gcaagggtga tccccgccgc
      961 aaggaataac acctgcgagg tgatcatcaa cgtacgtggc ttaaaaattc ccagcgcaaa
     1021 tggcgtcgag aatgaaaagg tcatgctgac gccagcggtc agcgccataa tggtggcgta
     1081 ttctccgcgc tcaaacccca tgatttccat cagcaatacc ggcgatgtat tgacgaaagt
     1141 gaggatcacc gaaacgctga gggtggtgat aacaacacgg ctgaggaaaa aacgattaag
     1201 cagcgactcg ctattttctc gtggtttgtc cgaagcagcg ggggacgctg ggcgcgtttc
     1261 ttttaaaata aacaaagaca acatcagtac cgcgatgccc atcattgcca tcgcccagaa
     1321 cagactctgc cacgggaatt taagcataat cagatgtccg agcactggcg ctaacaccgg
     1381 aatgatgcag gtaataccgt tgagtaatga cagcacttta gcccgacgtc gatcatccag
     1441 cgtgtcgcgc aaaatggcga acgccaccac gtaacaacag cctgcgccca acccctgtag
     1501 aaatcggcct gcaagaaata acgtgctggt ttcagccagt gaacagaaca ccgaggcaat
     1561 aataaatagc gccgcgccgg gtatggcgac cggctttctc cctgaacgat cggccacttt
     1621 accggcaaat agcatcgcag ctgccatccc cgccagatat acggagaacg caatatgcaa
     1681 ctgcgcttcg ctggcattga gatcggcggc gatgcgcggt aaaccaacga ggtacatatc
     1741 aatcccggcg ggataaagta aaaccagggc aaaactacaa atcaaaaagc gggacatggg
     1801 ctaaagcgtc ctgatagtaa ggtgacgcaa agcatacgtg gtgaaggagg ataaggcgag
     1861 ttgccatttg gacataagtg atttcctgag aggcaagaag ccagcgaatg gctggcttct
     1921 tgaaggattt agccaaattt aggtaacacg ttaaagacgt tgccgaacca gcacaaaatc
     1981 acggttatac caaagagaat gacaatcgcc ggaataagat tgccgcccca gaccgtgaac
     2041 atctgattgg gaaacttctt gcgagctttg attgcaagca ctgcgggaat aatgaccgcc
     2101 cagatggtgg cgcacagccc ggcaccgccg atcccgtaaa taaagccgtt cgggaagatc
     2161 agatacaaca acgcaggtgg caggaaggtt aacagcacgg ttttgaaacg cccgccgtgg
     2221 gagttatcaa tcttaaacag gtccgccaga taatcgaaca accccagcgt gacaccaaag
     2281 aacgaactgg caacagctaa gttagagaac accagcaggc aaaactcgat aatgccgtgc
     2341 tgtttggtgc cgaggaacga tttcaccagc gaatcaatgt tgccgcctga ggagattatc
     2401 gccttaaagc tttcgcgcgg aatattcccc atcgtgcaat agagccagaa gagataaatc
     2461 accagcgcca gcagcgaacc aaataccacg cttttgatta acttatcttt gcgtttgcca
     2521 tagcaaataa tcaggctggg aatattgccg tggaaaccaa atgacgccag acacaccggc
     2581 aaagccataa agatatacgg gaagtaagac gttcccgcag tggtgctggt ggcatcgcgc
     2641 aggatggagt aatcgacctg gaagaagaaa gaaccaaaca cgatcacaaa ggagataatc
     2701 ttcagcccga ggaacaatga ggtaatgcga ctggcggcta acgaacttat ccacaatacg
     2761 ctggcaacga aaatggctgt gcagatcccg acaatacgtg gattagcgtg atagcccaaa
     2821 ttcattgata tcgtttcact aatgatcgca ccattagcag ag
//
LOCUS       DAZPAB010000310         2828 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZPAB010000310 DAZPAB010000000
VERSION     DAZPAB010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.312"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            67..585
                     /gene="mddA"
                     /locus_tag="ACHUPG_004045"
     CDS             67..585
                     /gene="mddA"
                     /locus_tag="ACHUPG_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR0863418.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            582..1031
                     /locus_tag="ACHUPG_004046"
     CDS             582..1031
                     /locus_tag="ACHUPG_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0863419.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1032..1265)
                     /gene="ydcY"
                     /locus_tag="ACHUPG_004047"
     CDS             complement(1032..1265)
                     /gene="ydcY"
                     /locus_tag="ACHUPG_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR0863420.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1351..1524)
                     /gene="ortT"
                     /locus_tag="ACHUPG_004048"
     CDS             complement(1351..1524)
                     /gene="ortT"
                     /locus_tag="ACHUPG_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR0863421.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1719..1814
                     /gene="yncL"
                     /locus_tag="ACHUPG_004049"
     CDS             1719..1814
                     /gene="yncL"
                     /locus_tag="ACHUPG_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR0863422.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2216..>2828)
                     /locus_tag="ACHUPG_004050"
     CDS             complement(2216..>2828)
                     /locus_tag="ACHUPG_004050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFR0863423.1"
                     /translation="EYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIASNVKIMGG
                     NHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWIGNDVVLKG
                     GIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIKWWNYNYSD
                     LPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
BASE COUNT          745 a          628 c          621 g          834 t
ORIGIN      
        1 ccgttgcttc gggctgtttt cgatagtatt gataacatca tcatcattat tttattgagg
       61 ccagacatgt ccatccgttt tgcccgcaaa gccgactgtg ctgccattgc ggaaatttat
      121 aaccacgccg tgttgtatac ggcggctatc tggaatgacc aaacggtgga tgcagataac
      181 cgcattgcct ggtttgaagc gcggaccata gcaggttatc cggtgctggt aagtgaagaa
      241 gatggcgtag ttacgggata tgcctcgttt ggcgactggc gtagtttcga tggttttcgc
      301 cataccgtgg aacattcggt ttatgtccat cccgatcatc agggcaaagg tctggggcgt
      361 aaattgttaa gtcgattgat tgatgaagcg cgggattgcg ggaagcatgt catggtcgcc
      421 gggatcgaat cgcaaaatca ggcttcgctg catctgcacc agtcgctggg atttgtcgtc
      481 accgcacaaa tgccgcaggt aggcactaaa tttggtcatt ggctggatct gacatttatg
      541 cagttgcaac tcgacgagcg cactgaaccg gacgcgattg gatgaatcag tcgctcaccc
      601 ttgctttttt gattgccgcc ggaattggtc tggtagtcca gaacacgctg atggcgcgca
      661 tcacccagac ctcctctacc attctcatcg ccatgttgct gaactcactg gtggggattg
      721 tgctctttgt ttctatttta tggtttaaac aaggcatggc ggggtttggc gaactggtgt
      781 ccagcgtgcg ctggtggaca cttatacctg gcttgctggg ttcgtttttt gtcttcgcca
      841 gtatcagcgg atatcagaat gtgggagcgg cgacaactat tgcggtactg gtcgccagcc
      901 agctgattgg cggtctggtg ctggatatct tccgcagtca tggcgtacca ctacgggcgt
      961 tgttcggccc aatatgtggc gcaattttac ttgtggttgg cgcctggttg gtggccagac
     1021 gctcatttta atcagaggat ggtgccgcct ttggttaact gttcgtggcg cgcttccata
     1081 tcttctttat gcttgcgacc gtgatgagca atagcggtgc gcaaacgctg ctgctgggtg
     1141 tagcgatctt cccgacttaa ctccgcgtca tcgctcaggg cgattaataa ttcgttcata
     1201 tgggcaatga cgctctcttc aatcgcggca tccacgcgcg cgatgacttc atccagatgc
     1261 gacatacttt ctccttagcc acttgtgaaa tttatcgatt aatccaaatg atataagtct
     1321 gcgtggtgcc gtcaagccag tgagtaagat ttaaaacagt gaaaacaaca gcgccaccgg
     1381 gaaactcatc ggccatgtgg cccccaccag aaaggcgctt aagaaacgga tgcgtttctt
     1441 atcgtgagaa agaaaccagg tgataagaaa tgcgattgct gccataaccg cataaaaaac
     1501 aagcatatgt tgatagagaa acatgtacgg gcgtccagcc atatgaataa aacgcgcgca
     1561 aatatgcgcc attttttgac gtgcatcaag ttttttcacc agtttgagaa aaaatcatca
     1621 aacagcaact tgcccttatc catcttcaga atgagtatta ttgcggattt tcttaacccg
     1681 tactatacac ataggggcaa tgataaaagg tggcaaaaat gaatgtttcc agtagaactg
     1741 tagtactgat aaatgtcttt gctgctgttg gtttgtttac tcttatctct atgagatttg
     1801 gctggtttat ttgatgtagt gattttgcac gcagcacgta ctgtcagaga aatcaactct
     1861 ctgacagtac gcaagatctt ctcatactct ccaagtttgc aacattatcc ttaatattta
     1921 cttctttttg ctggcatcta cattgaaatg agtagaaaac atccccctac tgtcgtctat
     1981 acaaacagct tactgattca aacggaatct tgttttcaca tttttctatc acgaatactt
     2041 tatacgcgcg ggagtctata aatgctttat actttcaagg aataacgtaa atataaaaag
     2101 atggcgtaaa gtatatgcat ggcaggatgt ttgcaacctc cttgatgacg ctgccccatt
     2161 gaaattgcaa accccgccct ggttatcttg cccatttatc atgcaaaaca gataattata
     2221 ggcctctgaa tacctcagaa agattgaatt ttttatagtc cctctcttga atatttccat
     2281 ttgatatcaa tcgattcatc tcttcaacaa aataatttat atcatcacac ttattattat
     2341 ccggaagatc tgagtagtta taattccacc attttattct caacagttca tcaataacat
     2401 ttgaatcaaa cctaaatctg attatttttg caggaactcc tgcaactatg gcatatgggg
     2461 gcacatcttt ggtaacaacg gaattggcag caataacagc accatcacct attgcgatgc
     2521 cacctttcaa tacaacatca tttcctatcc agacatcatt tcctataatc ggattttcag
     2581 gtttggtaat gaatggtttc aatgtccagc tacgttcaaa ttcactcata gcaaacttat
     2641 caaattcacc gttataagtc atcatgtgag tagtaaaacg attcaaagga tgatttccac
     2701 ccattatttt tacatttgat gcaatactac aaaatcgacc tatacgtata ttccctgaaa
     2761 aatggcaggt cggaaatgaa aaagcgccga cactggagaa atttttcaca ggcattgttg
     2821 aatattct
//
LOCUS       DAZPAB010000311         2823 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZPAB010000311 DAZPAB010000000
VERSION     DAZPAB010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.313"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            376..815
                     /gene="ycgY"
                     /locus_tag="ACHUPG_004051"
                     /pseudo
     CDS             376..815
                     /gene="ycgY"
                     /locus_tag="ACHUPG_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
     gene            complement(894..2591)
                     /gene="treA"
                     /locus_tag="ACHUPG_004052"
     CDS             complement(894..2591)
                     /gene="treA"
                     /locus_tag="ACHUPG_004052"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR0863424.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
BASE COUNT          692 a          663 c          694 g          774 t
ORIGIN      
        1 cattttatta atgcatttct cattcgggtg aaatttccgc tacctggacg cataaaaccg
       61 gaactctcgc caaaagtcgg gcactccgaa ggaattgacg cgttgcgctg ctctgtaacg
      121 gcatttaccg caagccattt atttcgccag cgacaatccg cagagaacca aacgcatcag
      181 gaaacgactg aacacaataa atactgtatt gatcaacata ttatactggc acctccagca
      241 cgcagtgagg atacactgta tcatcagcga atcttatcac tgcggagctg gttgaacgac
      301 tttctgagcg aaataagtcg gttcccggta acaacaacag aatccttttt taattattgt
      361 ttcgttgttt ttggtgtgat tcagaaaata aatattttag acagtagcca gtcaggcgtt
      421 aaaaatgggg cggatagtga ggcggttttg gctgcgctta ttgaacattt agaattgatc
      481 aatccttctg gcagattaag ccaaaatacc cgttcggcaa tgcttcagtt acgcgaagag
      541 gaatggtctg aattcttttt ttggttgcta aattctctgg agtgtctgga ttatgtgatt
      601 attaatttaa ctccagaaag caaaaaaacg ttgatgagtg aacaccgtaa caatattcgg
      661 tagcaattga tgctttatat agccaacgtc ggcgtaaatc gccgggtgat gaaagtgaaa
      721 cacttacccg tagaaatgac gctatcttcg gcaaccacgt atggcaaaca tttgcgcaat
      781 acttcccgcc aggacttgaa aaaccgtctg tgtagaaaaa accatagata tggtgttatc
      841 tgccgacaac gccatccgga gaagccgaat gacgttgtcg gagtaagcgc tggttaaggt
      901 gtgggttgtg cctctttggt tgagggttgc gtcgttgctg acttaacggt cggatgcgtc
      961 gccggaacat tgtcacacgg ttgctctttc gggcagatca aatccagcat tttcagcgtc
     1021 acgccattgg tccagccaaa gccatcctgt aatggatatt cgccaccgcc gccccccgtt
     1081 ccggtggtgc tgacatcata tttttccacc agctttttct cccggtcata ggtgtgctga
     1141 acattggtca ggaagtgcca gctaatgtcc atcgccacct ctttttgccc gtagttttgt
     1201 aatccttctg tcgcgaccca ctgtaacggt gcccagccat ttggcgcatc ccattgttgc
     1261 ccacttttca ccgacgtggt gttcaggccg ccgggttgca gcagatgtgt tttcgtcgcc
     1321 gtcgccattt tgttggcgcg atctttcgct gccgcattga cgtacagcgg gaacagggcg
     1381 gccgcggtta actgattgcg cactttatga cttttcaggt cgtaatcggc ataccagcct
     1441 tgttgatcgt tccacaggta tttttcgatc cctttttgac gggcattcgc cagcgtttcg
     1501 tactggtttg ccatcgcgtt atctcctgca gctttgctgg cgcgggcgag gattttttcc
     1561 attttaaaca tcaggctgtt cagatcgacc ggtacgatgc tggtggtgcg taaggtattt
     1621 aactgctgcg ggttgtccat ccagcgcgag ctgaaatccc agccagacgc agcggcagag
     1681 cgcaggtcgc ggtaaatttc agtggcaggt cgattcggat tgcttttggc ggtggcaata
     1741 tcttccaccc atgactctgg tcgtggcgta tcgcgatcgt cccagtagcg gttgagaagg
     1801 gtactatcct gaagttttac aacgcgtttt tcctgttgtc cggcttgcag gttttcaaca
     1861 ccgtccatcc agtaagcata ttctttttgc atttgcggca ggtattgctt caacgcggca
     1921 tcgccttcat gctgcgccag taactctacc atcagggcaa agaagggcgg ttgcgagcgg
     1981 cttaaatagt aactgcggtt gccgttggga atatgaccgt aagtgtttat ttcatgagca
     2041 aaattggcca ccatatccgc gattttatcc cagtgaccac tttcggcaag tcctaacatg
     2101 gtgaagtaat tgtcccagta atatacctcg cgaaaacgtc cgcccggcac gacataaggt
     2161 tccggcagcg gtaacagaga atcccatttt tcggtgtttt cggtagaacg cgttaatacc
     2221 ggccaaagtc cgtcaatatg ttcgcgcagt gactgcccct ctggcggaac atatttctcg
     2281 ccttctttcg gcagggtgaa attgacgtta acgaaatggc gcagatcaaa tccgctctgg
     2341 ttttgctgca tccgataatc agcaaggatc atcagcggat cgctgttagg cacggcatcg
     2401 gcaaaggttt tttggtccgg aaaaagtttg acgttttgca catcattaaa cagcggccct
     2461 aataaaatat caggcggctg tggtgttacc ggtgtttctt ctgcctgcac cgatagcgca
     2521 gcgaaacaca aaaagataca ggctggaatt aacgccattt tttgcgggcg agaaggtgcg
     2581 ggggatttca tcaatcattc tcctttggcg aaaccgaata aaacggtatt caagtatcag
     2641 aaaaccttag ttcaggatcg cactagctgc atgatcgaaa tctcattctg cgaacggtca
     2701 ggcattttag aaaagggaat taaagggata aataacggcg aaatagagtg atcaaataac
     2761 gtctgtttat tagccagcca gcgacatttg gttcgcgatt agcgaagaaa aatcatcata
     2821 aat
//
LOCUS       DAZPAB010000312         2813 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZPAB010000312 DAZPAB010000000
VERSION     DAZPAB010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.314"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            39..671
                     /gene="comR"
                     /locus_tag="ACHUPG_004053"
     CDS             39..671
                     /gene="comR"
                     /locus_tag="ACHUPG_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR0863425.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(733..1272)
                     /locus_tag="ACHUPG_004054"
     CDS             complement(733..1272)
                     /locus_tag="ACHUPG_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR0863426.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1499..2803)
                     /gene="ndh"
                     /locus_tag="ACHUPG_004055"
     CDS             complement(1499..2803)
                     /gene="ndh"
                     /locus_tag="ACHUPG_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR0863427.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          677 a          777 c          687 g          672 t
ORIGIN      
        1 ataacctttc attcacagtc accaggtaca acggcatcat ggcaactgac tcaacacaat
       61 gtgtaaaaaa aagccgtggc cgcccaaaag tgttcgacag ggatgccgcg cttgataagg
      121 ccatgaaatt gttctggcaa cacggttatg aagcgacttc tcttgcggac ctcgtcgaag
      181 caaccggagc caaagcgccc acgctatacg cggaatttac caacaaagag gggttatttc
      241 gtgccgttct cgaccgctat atcgatcgtt ttgccgctaa gcatgaagca cagctgtttt
      301 gtgaagagaa aagcgtggag tctgcgctgg ctgactattt tgctgccatc gccaactgct
      361 ttaccagcaa agacaccccg gcaggctgct tcatgatcaa caactgcacc accctctccc
      421 cagattcagg agatatagcc aatacgttga aatcacgcca tgcgatgcaa gagcgcactt
      481 tgcagcagtt tttatgtcaa cgacaagcgc gcggggaaat cccgacccac tgtgacgtga
      541 cacatctggc agaattcctt aattgtatta ttcaggggat gtcgatcagc gcacgcgaag
      601 gtgcatcgct ggaaaaactg atgcagattg ccggaacgac tttgcgttta tggcccgaac
      661 tggtgaaata agtcgcaaaa aaggcccctc agcctgagcg aatgaggggc caaattgcag
      721 agtacagcct tgttatactt tgttattcaa aatcagttgc ccgttactat ctagcgggat
      781 ctgcgtaccc ggatcgcggt ccatgcggat tttgccctgc tgatcgccaa tcttataggt
      841 cacatcataa ccgagcattt tttctgactt gtcatacacc gttttacaac gctgttgcgt
      901 agtcgtgtaa gtatcgcttt cctggagaga gccctggatc tggttaccgg catatccacc
      961 acccagcgcc cccacaacag tggcgacatc tttaccgcga ccaccaccaa actgatgccc
     1021 tatcacgcca ccagcaacag cgccgagcac cgacccggta atgcgatttt catcctgcac
     1081 cggtcgacga tgggtcactg tgacgttgcg acactcctga cgcggtgttt taaccgtttc
     1141 cttgattggg gttgcagaaa caacctgagc gtattgcggg ccccgttcaa acacgttcag
     1201 actggccact gccgctacgc ccaacgcagc tgcgacacca atcccgatac ccgccaacat
     1261 tgatttattc acgggaacac ctccttcttt gcagttatgc caatcatgca agcgaaggag
     1321 aaaagcagct atcagacaga agatgaaaag cgcgggaatg actggaaata cttaaggatt
     1381 cggaggagtc tgaaggaatg cctgatgcga cgcttaacgc gtcttatcag gcctaccgct
     1441 cgtaccattt gtccagggcg gataaggcgt ttataccaca tccgccagtg tacgtcgatt
     1501 aatgcaactt caaacgcgga cggataacgc ggttaatact ccccaccagc atcattaatc
     1561 cggttttaaa gtaaccatgc agcgcaatct gatgcattcg gtatagcgag atatatacaa
     1621 agcgcgcaat tcgtccttca atcatcattg agccgcgcgt caggttaccc atcaggctac
     1681 caacggtgga gaagttcgac agcgatacca gcgaaccatg atctttatac tgataatttt
     1741 tcagcggctt accgttcatc tgcgccagaa tgttgttcat tgcgcaagtc gccatctggt
     1801 gtgcagcctg agcacgcggc ggaacaaagc ccccttccgg acgcgggcat gacgcgcagt
     1861 cgccaatagc gtaaatgtct ggatcgcgag tggtttgcag cgtcggttcc accaccagct
     1921 ggttgatacg gttagtttca agaccaccga tatctttcag gaagtctggc gctttgatcc
     1981 cggctgccca taccatcaga tcagcctcaa tatattcgcc atctttagtg tgcaggccgc
     2041 cttcatcagc actggtgacc atggtttgcg tcagcacgcg aacgccaagt ttcgttagct
     2101 cgttgtgggc cgcagcagag atacgtggcg gtaatgcagg caaaatacgt tctcccgctt
     2161 ctaccagcgt tacgttcagg gcttcgttgg tcaggccttt gtaaccgtag ctgtgcagtt
     2221 gcttgatcgc gttgtgcaat tcagcggaga gttctacacc cgtcgcgccg ccgccgacaa
     2281 tcgcaatgtt cactttgcca tttgcgccca ggttggcaga gtatttcagg aacagattca
     2341 gcatctcctg gtggaagcga cgcgcctggt gcgggttatc gaggaaaatg cagttctctt
     2401 tgacgcctgg cgtattgaaa tcgttagagg tgctacccag cgccatgacc agggtgtcat
     2461 aggcgatttt acgttccgga accaacagtt cacctttctc gtcgcgtagt tctgcaatag
     2521 tgattgtttt cgcttcacga tcaatatcaa tgacggaacc caactggaac tggaaaccat
     2581 gattgcgcgc atgggccaga tagctcaacg catcgacgcc ttcatcaagc gagccagtcg
     2641 ccacttcgtg cagcagtggt ttccacaggt ggctgtggtt acgatcgacc agcgtaattt
     2701 tggctttttt cttacgtccc agcttatgcc ccagctgtgt tgccatttcc agcccaccag
     2761 caccgccgcc gacaatcaca atctttttca atggcgtagt caacgtgccc ccc
//
LOCUS       DAZPAB010000313         2807 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZPAB010000313 DAZPAB010000000
VERSION     DAZPAB010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.315"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            6..81
                     /locus_tag="ACHUPG_004056"
     tRNA            6..81
                     /locus_tag="ACHUPG_004056"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHUPG_004057"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHUPG_004057"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACHUPG_004058"
     CDS             complement(299..1057)
                     /locus_tag="ACHUPG_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR0863428.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACHUPG_004059"
     CDS             complement(1065..2168)
                     /locus_tag="ACHUPG_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR0863429.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2807)
                     /locus_tag="ACHUPG_004060"
     CDS             complement(2178..>2807)
                     /locus_tag="ACHUPG_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFR0863430.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          763 a          739 c          675 g          630 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaataacgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaa
//
LOCUS       DAZPAB010000314         2803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZPAB010000314 DAZPAB010000000
VERSION     DAZPAB010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.316"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            95..2248
                     /locus_tag="ACHUPG_004061"
     CDS             95..2248
                     /locus_tag="ACHUPG_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFR0863431.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2301..>2803
                     /locus_tag="ACHUPG_004062"
     CDS             2301..>2803
                     /locus_tag="ACHUPG_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863432.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPD"
BASE COUNT         1026 a          507 c          513 g          757 t
ORIGIN      
        1 tcgcttaatt ccatataact ggacgattac attagaagta aactatactt tttatgaaat
       61 atcagggata aattattcac atggaaaata catcatgtac aaatcattat ttactattat
      121 cattctactg tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat
      181 aactttcatt gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat
      241 gaaactcgtt cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc
      301 aatttctgct cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa
      361 aaaattagaa actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga
      421 cggtaatggc actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc
      481 tgaatataca aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa
      541 acgtctggca atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg
      601 gaaagtcacc ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc
      661 atttgaagga tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa
      721 atcctggttt gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag
      781 agatggaaaa tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt
      841 ttctaatggt cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag
      901 taaacgtcat tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga
      961 tgggaaaatc aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga
     1021 atattatcct gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg
     1081 aaacgatacc agttactacc aatccggaaa aataagctct atacacaata gaaattcccg
     1141 tggtcagtat gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc
     1201 cgtctataaa gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga
     1261 agaggagaaa cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa
     1321 aaatggtaat cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga
     1381 agagtggtac gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga
     1441 cggcattgcc aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa
     1501 tggcgtagag aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga
     1561 aaccctgtac gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg
     1621 taaactatta acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta
     1681 tgatcaaaac ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt
     1741 agacaatcca acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc
     1801 attagggaaa tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa
     1861 atctgcagag caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa
     1921 tggtatacct gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg
     1981 cagtagtaat gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca
     2041 gaatcttcct gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc
     2101 acatttctat ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa
     2161 agccaggttt catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt
     2221 aaatgaactt gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac
     2281 agaaaaggat atagtttgcg atgaaagcga aatccacttt gttattaatg attttatctg
     2341 ttttttattc tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg
     2401 tcgagaaaaa actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg
     2461 ctgaatggtt taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca
     2521 atttactgag caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg
     2581 cctccagagt cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg
     2641 ggttaaataa accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa
     2701 gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt
     2761 atttaccgac tgtgttagtc gcatatcaga tcgttcctga taa
//
LOCUS       DAZPAB010000315         2799 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZPAB010000315 DAZPAB010000000
VERSION     DAZPAB010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.317"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..98
                     /locus_tag="ACHUPG_004063"
     CDS             <1..98
                     /locus_tag="ACHUPG_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0863433.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            156..512
                     /locus_tag="ACHUPG_004064"
     CDS             156..512
                     /locus_tag="ACHUPG_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863434.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            605..823
                     /locus_tag="ACHUPG_004065"
     CDS             605..823
                     /locus_tag="ACHUPG_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR0863435.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            825..1190
                     /locus_tag="ACHUPG_004066"
     CDS             825..1190
                     /locus_tag="ACHUPG_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR0863436.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            1187..1852
                     /locus_tag="ACHUPG_004067"
     CDS             1187..1852
                     /locus_tag="ACHUPG_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863437.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1852..2217
                     /locus_tag="ACHUPG_004068"
     CDS             1852..2217
                     /locus_tag="ACHUPG_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR0863438.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2672..>2799
                     /gene="hokD"
                     /locus_tag="ACHUPG_004069"
     CDS             2672..>2799
                     /gene="hokD"
                     /locus_tag="ACHUPG_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR0863439.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAV"
BASE COUNT          790 a          575 c          749 g          685 t
ORIGIN      
        1 ggcgcctgaa tataatctgt cgcgagtgcc ggaagagcga agctatgcag cgtgtactgg
       61 ctttctatca gggtaatttt caggaggcga tactgtgaat gaaattagct atcaggcttc
      121 aattaccgct ggcattcgca tcaaaggaga ggagcatgga aataaaacca gaagatgagt
      181 taagcaatat cgttttattt ccgataaaag aggatgaccc tcgtaatcag gttaattttc
      241 tttataagcc atcggaaaga ccatattgcc atcacgcctc tgtacgggtt gacgaaaaag
      301 agcgtcaggt ccgctgtaaa atctgcggtg cggttgtgga gccgtttgac tggatgctct
      361 ctgtggcaaa aagagaaacc agactggcag atgatgtaag gctattgcgc caggaggaac
      421 aggaaaggcg gagaaatata gaaaagctga tacagattga gcgtaacgcg aaagcgcgga
      481 tacgcagggc gacaaaatcc agaactgaat aattaaattt agcactgtta aaaatttaat
      541 ccttaaccgg agggatttct gcaccctcaa atcatcagga gaccgcccga aagggcggta
      601 atgaatggtt acattattta gaaaaaaata tccgcgaaag agtagaacaa cagaattcct
      661 gtttctcatt ctgtttatcg tgttgatgac accgatatcc ccgcaaattt ttgtctggat
      721 aatcggaaaa ataattgagc cagttattga attgtataac gacgtggtat gggcgtcatt
      781 caacacactg cacaataaaa ttaatccgta taaggaaagc tgatatggca ctgacgaaaa
      841 aacaacgtgc agaactgcgc atgaagttcg gtggtcgctg cgcttattgc ggctgcgaac
      901 ttggcgaaaa gtggcatgca gaccatgtaa aaccggtcat tcgttttgat ggaaatatgc
      961 ttcaccagga acgtgacgat atatccaaca tggtaccaga atgccaccca tgcaatctgc
     1021 acaagcattg cagtagcctg gaagattatc gacgaattat cagtgatggt cgtcgtgaat
     1081 tccttgcgtc cgggaaaggc aaagcgctgg ttcgtatggg attggttgaa atgaaatctg
     1141 acccggttgt gttctggttt gaaatatatc aagaaggggc tacggcatga ctactattac
     1201 caaagagcga ctgcaatggc tggctaacat ttctggccgc gatgatattg acgatataga
     1261 cggcggtgaa attcgtgaac tggcgcttat cgctctggca tcactggaag cagagcctgt
     1321 agcggaatgc attgttgaag atgggggcat gtgtattgat gggttcggtg agtatgtggg
     1381 tcactcgctg cctgatggaa tgcatgagct ttatgctgcc ccgcgaatgc gacaacctga
     1441 tggatggaaa gccgtagctg tagcgtggaa ggtgacgttt actcaagttg accaggaatc
     1501 taatacgttc actgctatat atttttacaa agcggaagtt gagcgttggg tacgactgca
     1561 taaagcatgt gattttcggg cagatataac accgctttac gcagcgccgg cagtgccggt
     1621 tgcagtaaac gacgacatgg cttacgcatt ccatcacgca ctgtcagatt catcgcttgg
     1681 ctctgatgaa atcgaagaaa ttaaaaccgg tttgcgtgct gcctttgcca acgtcactat
     1741 ccaaccagag ccgatagtgc cggatgaaat cgggccaaac gatagtaata cgtttgatta
     1801 tgttgatggc tggaacgcct gccgcgctgc catgcttaag ggagataaat aatgattaat
     1861 cgaaccaaac tggagcacat tctcgaatat gccaggcagc agaaatgtat tgggcaactt
     1921 tgtaaaattc caccaggaga tatggttgaa atcgtggaaa tggccatgcg taaggctggc
     1981 aactctccgg taactctggc tggctggata agctgtagtg atgcagttcc tgctgaatat
     2041 tgcgatgtga ttcttctcga tgatctcggg aatgtattcc ccggttcctg ggataaggtt
     2101 ttttgcccca ctcgtggcgg gaataagatg gcttttgtgg acaaagacgg cgttgaagta
     2161 gagagctcaa ctcactggat gccgctaccg gaaccaccgc aggaggtgaa tcaatgacct
     2221 ggcctgaggc attcaccacg gtaggaattg cgatggcggt ggcgctggtg gtgtattcga
     2281 tttgccgttg gggttaacaa acaaaaaccc cggattgatg gtccggggtt tttgaaggaa
     2341 acaaaacaga aacagcaatt gccgttacct gttgttacca tggcaagtaa acgtatctca
     2401 ggcgagcgca ttgcgccgtt ctgacgcaga taaattagcc tggatagaag gtgctggcaa
     2461 taaaaaatag cgttttctta tcggtatcgg taagattgct gcgggtgctt gaggctgtct
     2521 gcctcgggca tgccactgta aggcagacag agaaaagccc cagttaacat tacgcgtcct
     2581 gcaagacgct taacattaat ctgaggccca atctatgtct cacaaatgta ggttagcctc
     2641 ttacgtgccg aaaggcaagg agaagcaggc tatgaagcag caaaaggcga tgttaatcgc
     2701 cctgatcgtc atctgtttaa ccgtcatagt gacggcactg gtaacgagga aagacctctg
     2761 cgaggtacga atccgaaccg gccagacgga ggtcgctgt
//
LOCUS       DAZPAB010000316         2760 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZPAB010000316 DAZPAB010000000
VERSION     DAZPAB010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.318"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..171)
                     /locus_tag="ACHUPG_004070"
     CDS             complement(<1..171)
                     /locus_tag="ACHUPG_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HFR0863440.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMATFAVMTPTY"
     gene            complement(277..1293)
                     /gene="phnD"
                     /locus_tag="ACHUPG_004071"
     CDS             complement(277..1293)
                     /gene="phnD"
                     /locus_tag="ACHUPG_004071"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0863441.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(1318..2106)
                     /gene="phnC"
                     /locus_tag="ACHUPG_004072"
     CDS             complement(1318..2106)
                     /gene="phnC"
                     /locus_tag="ACHUPG_004072"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR0863442.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            complement(2239..2682)
                     /gene="yjdN"
                     /locus_tag="ACHUPG_004073"
     CDS             complement(2239..2682)
                     /gene="yjdN"
                     /locus_tag="ACHUPG_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFR0863443.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
BASE COUNT          562 a          718 c          802 g          678 t
ORIGIN      
        1 gtaagttgga gtcattaccg cgaaggttgc catgttgccg ccgtctttaa tcagcgtaag
       61 cggggccatt tccgcgccct gccacgagac gaccagcacg tcgagaacaa cggcccagct
      121 cagaagcgag aaccagctac gcttgggtgg ggcgatggtg atggtttgca tgtttggctc
      181 cggtctgtag gccggataag gtgcttgcac cgcatccggc atcaacgcct acacattgtc
      241 ggatgcgacg ccggggcgtc ttatccgacc tacgatttac tgcaccgctt tactcaccga
      301 actcatcgcg cttaacgcgt tgttcaggcg gtccaggtca tccagctgcg cctgaatctc
      361 ggtggttttc gccagcttgt cctgctcatt cagcccttta ttgcctttca cgctttgtat
      421 ctctttaaac agtgcgagct ggcgaatcgg caccagttgc aggtcgctgg aagcgcggaa
      481 cggcgcccag cccaggcgtt ccagcaccgc tttctcttcc ggcgtttttc cgtagttcat
      541 aaagaagtcg tagatcttgt ctttggtagt ttcggacaga ttcttgcgcc agacgatcgg
      601 atcgcctagg atcagcggcg atttccagat caccttcagc tctttaagct tctccggcgc
      661 ggaggttttc agcttgtcga ggttttcggt gttgttggtg gcaacatcca cttgcttgtt
      721 ggcgacggcc agcgcgttgg tttcatgccc ggcgttgacg gtgcgcttga agtcgctggc
      781 ggagatattg tttttagcga agacgtagta accggggacg aggaagccag aggtggagtt
      841 aggatcgcca ttgccgaagg tgagatcttt ccgcttcgcc agcagatcgt tcaggttgtt
      901 gatcgggctg tctttgttga cgatcaacac gctccagtaa cccggcgatc catccgccgc
      961 gaccgtctgg gcgaagacct ggccattggc gcgatccacc gcttccatcg ccgacaggtt
     1021 gccgtaccag gcaatatcca ctttattgaa gcgcatcccc tggataatgc ccgcgtagtc
     1081 cggggcaaag aaggcgttga ccttcacgcc cagtttcttc tccatatcct gtaaaaacgg
     1141 cgtccattgc ggtttcaggt tttgctgtga ttccgttgaa ataatgccga aattcagcgc
     1201 cttttcctgc tcttcggcgt gtgccggggt taacagggtg ctgaggctga acatgctggt
     1261 gaaggccagc gaggcaatta tcttagcgtt cattcgtttt cctcaatgat ggggatatca
     1321 ggcagctttc gcgttctctt cgatgcggtt aatgctgcgg tagagatggt caaaacgttc
     1381 gttatcaaac agttggctgc tgccgtcgta gaagacgtgc ccctgacgca gggcgacgat
     1441 gcgttcgcag tagcgcaggg cgtaatccac ctgatgcagc gtgacgacca cggtgatgcc
     1501 gtcgttctgg ttgatgtcgc gcagggtgtc catcacgatg cgcgctgatt ccgggtccag
     1561 cgaggcgatg ggttcatcgg ccagaatcac tttcgcctgc tgcatcagcg cacgggcaat
     1621 cgccacacgt tgctgctggc cgccggagag ggtggaaacg cgctgatggg caaaatgcac
     1681 catgccaacg cgggtcagcg cctgtaacgc gcgctgtttc tgctcgccgg tgaaccagct
     1741 aaaacaggtg cgccagaacg gcgtgctgcc gagcgcgcca atcagcacgt tctccagtac
     1801 gctcaggcgg ttcaccaggt tgaattgttg aaagatgtag ccggtgttgg cgcggctttt
     1861 gcggatatcg cgggccaggc ggccttcgcg ctggacagtg cggcccagta gctcgatatg
     1921 gctaccggcg gatttatcgc cggtaatcaa accgcttaaa tgacgtaaaa gggtggattt
     1981 gccggaaccc gacggcccaa gcagagccac catttcactg tgatgaatgt tcagatcaac
     2041 cgcatgcagc gcttgatgct gattgaaggt tttggcgagc ttctcgacac ggataatcgt
     2101 ttgcatgatg cagcctccct aaaaaagtgg ctccatcgtg gcggattatt gtgacgattt
     2161 ggttaattaa aagtgactaa cagatgaaga gttaacggga attcgatgac agtgcgggag
     2221 ggcgggcctc ccggcgggtt attgcgttgg ttgttgtttg acgacattaa tcatccacgg
     2281 tacgccaaat ttatcggtga ctttgccaaa gccatgcgcc cagaaagttt cctgccaggc
     2341 catttcgatt tttccgttag cggcaaggtt atcaaaccag cgttttcctt cttcgacctg
     2401 ttgcgaatcg agcaccagcg taaagccgga gtagctggct tttcctgacg gtatagcatc
     2461 gctcatcatg atgtcgcttc cggcaatgcg cacgttggca tgagcgatgg cggtatcggg
     2521 aaattgcatt ccggaagggc agttctcggc gctgtcctgc gctgatttcg gcatttcgcc
     2581 gaagctgatt ttatagagca gttccgcgcc caacgtacgt tgataatagg caatcgcgtc
     2641 ggaacagtta ccggcaaaag agaggtaggg acttaacggc atgatggtga cctcagttaa
     2701 gagaagccag ttaagtgtag ttcgaaattt atacagatga gaggctgcct gataagacgc
//
LOCUS       DAZPAB010000317         2760 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZPAB010000317 DAZPAB010000000
VERSION     DAZPAB010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.319"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..194
                     /locus_tag="ACHUPG_004074"
     CDS             <1..194
                     /locus_tag="ACHUPG_004074"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863444.1"
                     /translation="LLFLINPSGILTLSYDYLGLGSLVSAYSRQKRISPLMAQILQWL
                     VKNELFLHYNLILTMIRAG"
     gene            236..1402
                     /gene="nhaA"
                     /locus_tag="ACHUPG_004075"
     CDS             236..1402
                     /gene="nhaA"
                     /locus_tag="ACHUPG_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR0863445.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1468..2367
                     /gene="nhaR"
                     /locus_tag="ACHUPG_004076"
     CDS             1468..2367
                     /gene="nhaR"
                     /locus_tag="ACHUPG_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR0863446.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2405..>2760)
                     /locus_tag="ACHUPG_004077"
     CDS             complement(2405..>2760)
                     /locus_tag="ACHUPG_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR0863447.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
BASE COUNT          641 a          612 c          700 g          807 t
ORIGIN      
        1 tattactttt cctaattaat ccctcaggaa tcctcacctt aagctatgat tatctgggct
       61 tagggtcact cgtgagcgct tacagccgtc aaaaacgcat ctcaccgctg atggcgcaaa
      121 ttcttcaatg gctcgtaaaa aacgaattat tcctacacta taatctgatt ttaacgatga
      181 ttcgtgcggg gtaaaatagt aaaaacgatc tattcacctg aaagagaaat aaaaagtgaa
      241 acatctgcat cgattcttta gcagtgatgc ctcgggaggc attattctta tcattgccgc
      301 tatcctggcg atgattatgg ccaacagcgg cgcaaccagt ggatggtatc acgactttct
      361 ggagacgccg gttcagctcc gggttggttc actcgaaatc aacaaaaaca tgctgttatg
      421 gataaatgat gcgctgatgg cggtattttt cctgttagtc ggtctggaag ttaaacgtga
      481 actgatgcaa ggatcgctag ccagcttacg ccaggccgca tttccagtta tcgccgctat
      541 tggtgggatg attgtgccgg cattactcta tctggctttt aactatgccg atccgattac
      601 ccgcgaaggg tgggcgatcc cggcggctac tgacattgct tttgcacttg gtgtactggc
      661 gctgttggga agtcgtgttc cgttagtgct gaagatcttt ttgatggctc tggctattat
      721 cgacgatctt ggggccatca ttatcatcgc attgttctac actaatgact tatcgatggc
      781 ctcccttggc gtcgcggctg tagcaattgc ggtactcgcg gtattgaatc tgtgtggtgt
      841 acgccgcacg ggcgtctata ttcttgttgg cgtggtgttg tggactgcgg tgttgaaatc
      901 gggggttcac gcaactctgg cgggggtaat tgtcggcttc tttattcctt tgaaagagaa
      961 gcatgggcgt tctccagcga agcgactgga gcatgtgttg cacccgtggg tggcgtatct
     1021 gattttgccg ctgtttgcat ttgctaatgc tggcgtttca ctgcaaggcg tcacgctgga
     1081 tggcttgacc tccattctgc cattggggat catcgctggc ttgctgattg gcaaaccgct
     1141 ggggattagt ctgttctgct ggttggcgct gcgtttgaaa ctggcgcatc tgcctgaggg
     1201 aacgacttat cagcaaatta tggtggtggg gatcctgtgc ggtatcggtt ttactatgtc
     1261 tatctttatt gccagcctgg cctttggtag cgtagatcca gaactgatta actgggcgaa
     1321 actcggtatc ctggtcggtt ctatctcttc ggcggtaatt ggatacagct ggttacgcgt
     1381 tcgtttgcgt ccatcagttt gacaggacgg tttaccgggg agccataaac ggctcccttt
     1441 tcattgttat cagggagaga aatgagcatg tctcatatca attacaacca cttgtattac
     1501 ttctggcatg tctataaaga aggttccgtg gttggcgcag cggaggcgct ttatttaact
     1561 ccacaaacca ttaccggaca gattcgagcg ctggaagagc gcctgcaagg caaattattt
     1621 aaacgcaagg gacgtggtct cgaacccagc gagctgggag aactggtcta tcgctatgcc
     1681 gataaaatgt tcaccttaag ccaggaaatg ctggatattg tgaactatcg caaagaatcc
     1741 aatttattgt ttgacgttgg cgtggctgat gcactttcca aacgcctggt cagtagcgta
     1801 cttaacgccg cagtggtaga aggcgagccc attcatcttc gctgcttcga atccacccac
     1861 gaaatgctgc tggagcaatt aagtcagcat aaactggata tgatcatttc tgactgtccg
     1921 atagactcta cgcagcagga aggcctgttc tccgtgagaa ttggcgaatg tggcgtgagt
     1981 ttctggtgta caaatccacc accagaaaaa ccgttcccgg cttgtctgga agaacggcga
     2041 cttttgattc ctgggcgacg ttcaatgtta gggcgcaaat tgcttaactg gtttaactcc
     2101 cagggattaa acgtagaaat cctcggcgag tttgatgatg ccgctttgat gaaagctttt
     2161 ggtgcgatgc acaatgcaat cttcgttgcc ccaacgcttt atgcatatga cttttatgcc
     2221 gataaaactg tcgtagaaat tggtcgcgtc gagaatgtga tggaagagta ccatgctatt
     2281 tttgctgagc ggatgattca gcacccggcg gtacagcgaa tctgcaatac ggattattct
     2341 gcgcttttta gtccagcggt gcgttaatcg gcagctcccc caaagttaag gtgggggaga
     2401 tagattagtt gtacattacc acgattttga ctcggctcat tatttgcccg cttgagacat
     2461 tgtttccata tgtacgcggg cgaataaata gaggaatctg attacttcct tcatggggat
     2521 gctgaaaaga gtagtaattg cttttaccat actggatttc attcgtacca tcattggaga
     2581 gttgaaagcc tatttgaccc gcaccgtcct cactgttttc taactcattt tttaatatgc
     2641 tggttcctga ctcctgtatc gtcactggtt caaagtaata ttcaacgtca ctgggttcaa
     2701 aaactaattc accttgctcg ccctggatgc agtcaaatct tatatgatga aactgataat
//
LOCUS       DAZPAB010000318         2733 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZPAB010000318 DAZPAB010000000
VERSION     DAZPAB010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.320"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..593)
                     /locus_tag="ACHUPG_004078"
     CDS             complement(<1..593)
                     /locus_tag="ACHUPG_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR0863448.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQ"
     gene            complement(660..1385)
                     /gene="fimC"
                     /locus_tag="ACHUPG_004079"
     CDS             complement(660..1385)
                     /gene="fimC"
                     /locus_tag="ACHUPG_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFR0863449.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1422..1961)
                     /locus_tag="ACHUPG_004080"
     CDS             complement(1422..1961)
                     /locus_tag="ACHUPG_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0863450.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="ACHUPG_004081"
     CDS             complement(2026..2574)
                     /gene="fimA"
                     /locus_tag="ACHUPG_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFR0863451.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT          707 a          699 c          602 g          725 t
ORIGIN      
        1 ttctgtacac tatttccgct gaaattataa ttgagcaatc cggcattaat accgggatcc
       61 cataactcag gaggaatata accacgcgcg cgattactca taaatgcctg agggatcgtc
      121 aggttcagtc gctgctgacc aacatccaga tgcgcagtag cgtcctggac cattgtggtt
      181 aatggcacac aggcatcatc cgccagcaga ttcataccgg cgacagaagc cgtattcagc
      241 cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa tcccttgttc actgtcgccc
      301 gtattaaatg tgacatcacg cgttgccata taaccattat tcaaatagat atcgacgcga
      361 tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg ataaatcggc cacagcctgg
      421 ggatcatccg ctaaaaagcg cagattaaaa tagaggtcgg cagatgacaa aggtgcctgt
      481 gcggcaaaag cacaggcgac aacgagtcgg acaaaaaaac cagccaaacg atgcttacga
      541 atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat tcagatatga cattattcct
      601 ccggtttaaa atcacccggc agtcaattct ttttattcag gcgaaaaatt ccccctgcgt
      661 tattccatta cgcccgtcat tttgggggta agtgcgccat aatcatttat tgttcggtaa
      721 gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc tttcgcccat tggaggcacc
      781 aatgcatttt caagaacccg ggttccggca ttcaactctg ttaccgtcag gtaatagggt
      841 gtcgggttaa tcagcgtcag agaattcgcg ctacgacgaa atcttaattt ttctgcggcc
      901 tgatcgggtg gcaacgctaa tttagccggg cgatagtaca gtttaatgcg gctgataatt
      961 gcgagctgta gcatattctc agtcaatttt gatttatcca ttgacggaat cgctttaacg
     1021 ttcatccaga ataaactttc ccggtcctgt ggcaattggt tatttgttgc atcaagaata
     1081 cgtaaggtat tctctttttt tcccttcatc gcaaacagag gaggcgtcac gataaaacga
     1141 ccatccttta caccatcggc attttccacc catgattgaa ttaaataggt actattttca
     1201 tcattatttg tcacggcaag ttgcacttgt ttttgccctg ccggataaat tacgcgagtc
     1261 gcacctaagg ccactcctgc ttcagcgcgt ccggcaacca tcattgccat gaacatcagg
     1321 atacctgcca gcaagcagaa tgttatttcc tgcgatttcc ttacattgac gtttttatta
     1381 ctcactgtcc tgttcctgtt atcacattat ctactgaaca attactgata ggttaaagag
     1441 aaccaggcct gggcattggc gatgccgcca gtaacccgac gcccggtagc acgatatttg
     1501 gcgatgaaat gtagcgaagt agagcctgag taaagccgtt tccagtttgc tggaggacga
     1561 ttaatcggta cgaggtttcc ttcatcatca aacaacgcta caccaatatt ggaggctatc
     1621 cctggcccct ctcccacgga gagcacatcc ggatttttac catccgcgac accgtgaaac
     1681 gccacaccca cacgttcact caccaccgtg ctacattccc gtaaatgaat aacaaaaggc
     1741 accggtgcgc tatcttcccc tgccgcatga aaccggttac tgctgatttg ccccatattg
     1801 accgtcattt gtttatcacc ggcttcaatc cggcaagttt ccgcaataat gacgccctga
     1861 aattgcatat ttccgccggg cagcgtggta ttccatttat ttccggccag agcaaacatt
     1921 ggcagcaacg acgctaacag aaataatctc ttccttttca tcgttatttt tatcgcacaa
     1981 gggtgggcat ccctgcccgt aatgacgtcc ttgaacctgg gtaggttatt gatactgaac
     2041 cttgaaggtt gcatccgcat tagctgcacc cggggttgcc tcgccgattg cataataacg
     2101 cgcctggaac ggaatggtgt tggtaccgtt attcagggtt gtttgtgcac taaatgtcgc
     2161 accgtccagc gtcagagcat tgcctgttct gtctaggatc tgcacgccaa cgtttgttgc
     2221 actacctgca gcagaactct gcagagccag tacatcagta cgcgtagcat caattgccgt
     2281 acctaagaag gcaacagcgg cttttgtggc aacagtggta tcgcaatcat tcagctgaat
     2341 gttaaaacca acggcagagc tggttgctcc agcctgcttc aggctagcgg tacgaacctg
     2401 tcccaactga acggtttgat caacagagcc tgcatcaact gcgcaagcgg cgttaacaac
     2461 ttccccttta aagtgaatgg tcccaccatt taccgtcgta gtatcggcca gagccgctgc
     2521 ggaactgagg gacagagccg acagaacaac gattgccaga gttttaattt tcatgctgct
     2581 ttcctttaaa aaaactattt ctaaatcgac atgggcagtc gttctgtaca ctttgttttg
     2641 tcaacgagtt tgactgccaa cactgcacag ttttccccca aaagatgaaa catttggggc
     2701 cattttgact catagaggaa agcatcgcgc aca
//
LOCUS       DAZPAB010000319         2731 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZPAB010000319 DAZPAB010000000
VERSION     DAZPAB010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.321"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(157..1581)
                     /gene="patD"
                     /locus_tag="ACHUPG_004082"
     CDS             complement(157..1581)
                     /gene="patD"
                     /locus_tag="ACHUPG_004082"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFR0863452.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(1603..2397)
                     /locus_tag="ACHUPG_004083"
     CDS             complement(1603..2397)
                     /locus_tag="ACHUPG_004083"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0863453.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(2387..>2731)
                     /locus_tag="ACHUPG_004084"
     CDS             complement(2387..>2731)
                     /locus_tag="ACHUPG_004084"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0863454.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT          699 a          806 c          640 g          586 t
ORIGIN      
        1 tatgctaatc aactgtgaca ggatgaaaaa tcgccatcaa acatttagcc taatttttac
       61 gccagttatt aatgtaatca ttctaatact taaccgccat taaactcaga acctaaaacg
      121 atagtggccg tcctataaca gccactaaca tctaacttaa tgtttaacca tgacgtggcg
      181 gacgacggtg taatcctcca gcccataaag tgacatatcc ttgccgtaac cagaaagttt
      241 ctgcccaccg tgcggcattt cacttaccag catgaaatgg gtattgaccc aggtacaacc
      301 atattgcagc cgtgcgctga cgcgatgcgc cctgcccaca tctttcgtcc ataccgaaga
      361 tgcaagtccg tactggctgt cattcgccca gttcaccacc tgttcttcgt tgtcgaaggg
      421 cgtaacactc actactggac caaatacctc tttttgcacg atggcatcgt cctgtaatgc
      481 gccagccagc agcgtcggcg catagtaata gccattaccc ttgcgctttt caccgccagt
      541 gatcactttg atgtgccctg tcgctttcgc ctcttctact gccttgctga cgcgttcgag
      601 atgcgccagc gagcttaaag gtccaagctc cgtagactcg tcatccggcg caccagattt
      661 taacgttgcc accgcagcac ccagtttttc caccagcgta tcgtaaatgc ctttttgcgc
      721 gtagatccga caagccgcag tacaatcctg tccagcattg taatagccaa atgtacgtac
      781 accttcgacc actgcttcaa tatccgcatc atcaaaaaca atcactggcg ctttgccacc
      841 aagttccata tgagtacgct taatggacga cgcggtatgg ctgatgatgt gctcgccggt
      901 ggcgatagag cccgtcagcg acaccatccg cactttggga tgaccggtca gcggatcacc
      961 caccgttttg cctctgccaa acagtatgtt aatcacgcct gccgggaaga tatctttcgc
     1021 cagctctgcc aacttcaacg cggtcagcgg ggtaatttct gatggtttaa gcactacgca
     1081 gttccctgcc gccagcgccg gagcaagttt ccacgcggcc atcatcagcg gataattcca
     1141 cggtgcgata gaagccacga cccccaacgg atcgcgacgg atcatcgaag tatgaccttc
     1201 aagatattca cctgccgcca gaccattcag acagcgcgcc gcacccgcga aaaagcgaaa
     1261 aacatcgaca atcgccggga tttcatcatt gaacgcacta tgcagcggtt tgccacaatt
     1321 acgggattcc agttcggcaa aaacctgacc attttcttcg ataacatccg ccagtttcag
     1381 caggcattcc gcacgcgctt tcggtgtggt ttgcccccat tcagcaaatg ctgcatctgc
     1441 cgcacgcaca gcagcatcga cctgctctgc ggatacctcg gcaatctcca gtaaaacgtc
     1501 ccccgttgcc ggattataga caggctgttt ttccccttcg ccgctaaccc gttctccgtt
     1561 aatcagtaac ttatgttgca tagcttattc ctgtatcagt ggttattgac cattgtcgcc
     1621 ttcgcgggtt agccaccagg cccccaggat cggcaaggtt gttaccaaca taaccagcag
     1681 tgccaccacg ttggttaccg gtacatcacg cggtcgccca agctgattga gcaaccacaa
     1741 cggtaacgtt cgttcatgac ctgccgtaaa ggtcgtaacg atgatttcat cgaacgacaa
     1801 ggcaaacgcc agcattcctc ctgccagtaa cgccgaactg agattcggca acactacgta
     1861 gcggaaggtt tgccagccat tggccccaag atccattgac gcctcaacca gactccacga
     1921 ggtgcggcga aaacgggcga tgacattgtt aaacaccacc actacgcaaa aagtcgcatg
     1981 accgaccacg atggtgaaaa atcccggctc cagattgatg gttttaaagg cggttaatag
     2041 cgccagacca gtgacaatgc ccggcagcgc aatgggcagc agcaataaca gcgaaatggc
     2101 gtttttgccg aaaaagtctc gtctccacag cgcggcagct gccagcgtcc ctaacaccag
     2161 cgcaattaat gtcgccagcg ccgccacttt aagtgacagc gtcacggcat caagaatatc
     2221 actacgctgt gctgccacgc taaaccagcg cagcgtcagg ccctgcggtg gaaaactaaa
     2281 cgccgcatct tcagtgttaa acgcataggc ggcgattatc aggatgggaa aatgtaggaa
     2341 aacaacgccg ccccaggccg ccagtttgag gaaaaacggt gcgcgttcag agtgcatcga
     2401 acgctcccag acgtttcacg aacgccaggt acagtgcgat gagaataatc ggcaccaggg
     2461 tgaatgccgc cgccatcggc atattgccaa tcgccccctg ctgggaataa accatattgc
     2521 cgataaaata tcctggagga ccaaccagct gcgggacgat aaaatcgccc agtgtgagtg
     2581 agaaggtaaa gatagagcca gcggcaatac ccgggattgc cagcggcagc accacatagc
     2641 gaaaggtttg tcgtggacgt gcgccgagat cagccgacgc ctgcaacaat gacggcggca
     2701 aacgctcaag cgccgcctga acgggcagga t
//
LOCUS       DAZPAB010000320         2715 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZPAB010000320 DAZPAB010000000
VERSION     DAZPAB010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.322"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            53..2575
                     /gene="lpfC-O113"
                     /locus_tag="ACHUPG_004085"
     CDS             53..2575
                     /gene="lpfC-O113"
                     /locus_tag="ACHUPG_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR0863455.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
     gene            2586..>2715
                     /locus_tag="ACHUPG_004086"
     CDS             2586..>2715
                     /locus_tag="ACHUPG_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0863456.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          727 a          586 c          679 g          723 t
ORIGIN      
        1 cagtgacccg ataaaaatga cgctgtaagc aagggataag gataggcgta ttatgatgac
       61 gaccagaata gtggttggcc tcacggcagg gacgtgtctg attttctcgc aaaacctgat
      121 ggccgaggtc agtgtattca atccggcgct tctggaaatc aaccatcaat ccggagtcga
      181 tattcgccag tttaatcggg caaacctgat gcccccaggt gtttatagcg ttgatatttt
      241 tatcaacggt aaaatgtttg aacgtcagga tgtgacattt gttcaggata atccagatgc
      301 tgatctgcac gcttgcttta ttgccattaa aaaaacactg tcctcctttg gcataaaagt
      361 tgatgcgctc aaatcgttca atgatgtgga tgagacggtt tgcctcgatc ctgctccacg
      421 tattgaaggc tcatcctggc agtttgacag tgataaattg cagctgaata tatccattca
      481 ccaaatctac atggacgcga tggcttatga ttacatcagc cccacgcgtt gggatgaggg
      541 gattaatgcg ctcaccatca actacgattt ttctggttca catacactac gttcagatta
      601 tggttcacaa gagacagata ccagttatct caatctgcgc aatggactga atattggacc
      661 gtggcggcta cgtaattaca gtactttaaa caccagcgat ggccgtgcgg aatacaactc
      721 cattagtacc tggatacagc gcgatattgc cgcgttaaga agccagatta tgattggtga
      781 tacgtggacg gcgagcgata ttttcgacag tacgcaaatt cgcggcgcgc gtttgtatac
      841 tgataacgat atgctacccg ccagccagaa tggctttgct cctgtggttc gtgggattgc
      901 aaagtccaac gccaccgtca tcattcggca gaatggctac gtgatttatc agtcagccgt
      961 tccacaaggt gcttttgaga tcaccgatct caacaccgca agtacaggtg gcgatttgga
     1021 cgtaaccatc aaagaagaag acggtagcga acaacgattc acccaacctt atgcttcatt
     1081 ggcgattctt aaacgtgaag gtctgacaga tgttgatgtc agcgtgggtg aattgcgcga
     1141 tgaagacgga tttacaccgg acgtccttca ggcgcaaata cttcatggtt tttcccacgg
     1201 gatcacttta tatggaggta tgcaggctgc tgaaaattat ggttctgcag ctctgggtgt
     1261 cggtaaagat cttggcgctt tgggcgcaat ttctttcgat gtgacacatg ctcgtgcgaa
     1321 ttttagccat gatgatacag aaacgggtca gtcatatcgc tttctctatt caaaactatt
     1381 tgacgacaca gacactagct tgcgcctggt tggctatcgt tactccaccg agggctacta
     1441 taccctcaat gagtgggcat cgcggcgcaa cagccctgaa gacttttggg aaacaggtaa
     1501 ccgacgtagt cgcgtggagg gaacgctaac gcagtcgttg gggagagatt atggcaattt
     1561 atacctgaca ttaagccggc aacaatactg gcataccgat gatgtcgaac gattaatgca
     1621 atttggctac agcagtagct ggaagcgtct ctcgtggaac gtctcctgga gttattccaa
     1681 tactgccaga caggggacgg ggaacaacca tgccagtgat aacaccagtg agcagatcta
     1741 catgctctct ttatctgttc ctttatcggg ctggtggggt aatagttacg ccacctattc
     1801 tgtttcgcaa aacgataatt ccggtagctc acatcaactc ggactcagcg gtacggcgct
     1861 ggaaagaaat aacctttcat ggaatttaat gcagtcctat aacagtcatg atgatgaggt
     1921 tggcggtaat atgtccctga cctatgatgg ctcttatggc acggtgaacg gcagctataa
     1981 ctacagccaa aattcccaga ggctgaatta tggtatcaga gggggaattc tggcacacag
     2041 cgaaggggta acgttaagtc aggagttagg tgaaactatt gctcttgtta aagcacctgg
     2101 ggccgccagg ttagaaatag ataatatgcg cggtgctgcg acggactggc gcggctatac
     2161 ggtcaagaca cagctaaacc cttatgatga aaatcgggta gcaatcagcg ataactattt
     2221 ctcgaagtcg aatatagaac ttgataatac cgtcgttacg atggttccca cgcgtggtgc
     2281 agtggttaaa gcggagtttg tgactcatgt gggttatcgc gttctcttca gagtgttaaa
     2341 tgcaaatggt aaaccggtac cttttggagc cattgctgcg atacaagatg caagtttggc
     2401 agattcagga attgtcggtg accgtggcga actttatctt tctggtctac cagaaaaagg
     2461 acaggttacg ttatcctggg gagaaaacgc ctcaacaaaa tgcatcttca attattcatt
     2521 ttcgacacca gaaagtgaga gcggattaat tgaacagggt gtgacatgtc attaaaagga
     2581 tttttatgaa caagtatatc aaacagtggt gctttgctgt gtttatgctc tcgttaagta
     2641 gtgtagcact tgcagctcct aaaggtatct gtacccctga taatggcgta tttcacagta
     2701 cgcttgattt ctcgg
//
LOCUS       DAZPAB010000321         2701 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZPAB010000321 DAZPAB010000000
VERSION     DAZPAB010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.323"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(322..1101)
                     /gene="ipaJ"
                     /locus_tag="ACHUPG_004087"
     CDS             complement(322..1101)
                     /gene="ipaJ"
                     /locus_tag="ACHUPG_004087"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HFR0863457.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(1298..2107)
                     /locus_tag="ACHUPG_004088"
                     /pseudo
     CDS             complement(1298..2107)
                     /locus_tag="ACHUPG_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2454..>2701
                     /locus_tag="ACHUPG_004089"
     CDS             2454..>2701
                     /locus_tag="ACHUPG_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0863458.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
BASE COUNT          807 a          677 c          438 g          779 t
ORIGIN      
        1 tttaaattgc gggtataaaa tacatctatt ttcaaataaa ataaagcata ctccaattac
       61 aacgagtgac taaaataaaa atatcatcca agtcaatcac tgtttgctct gcaaaatatg
      121 tcagccttct caaacttacc agcacttaaa tatttaagca cacaatcatt ccttgaggga
      181 gttcacttat ttcaagagaa acatttcgtc ttcccacaca actaatccgc ccaatatagc
      241 taaatttgta ctcagtaata tcatttgtta tgccccccac cccaaaatat tcaagctcct
      301 tcacagataa aaaacgacaa tttacaaagc ctcattagtt ataactatgg aaatacctat
      361 tttagtatat cctataaagt tagaattact tcttcttgca cctaattcca tagttgaaaa
      421 acaagaataa ttttctccta cagctggatc catatagctt ccatcaggtc tacacaacac
      481 ccagtggagg ccgacaggaa ctccaataaa ggatacagca acagcttcca aaactctttg
      541 gttagaagat aacacatctc gttgatgtac tatattacat cccatgccaa ttaataagtc
      601 ccttgcatcg ggataaataa aactaattac ttgcgaaaaa atgttacttt cttcaacgac
      661 ataagcattc aatccaagaa gcctcgtagc catgactatc ttatcaggca tagaataacc
      721 ggcatccgcg atattatctt tgtgtattct cggattataa ttaccactag ttattaagta
      781 taaatcccgc tcacaacgat tatccaaatc aagagaactc ttccttgtca tttcggacat
      841 agaccctttg tattgtggta ttttatcaac ccctaattcc tttgcagcac agagtaaact
      901 ggcagctcca caagaattgt catacatctg cttcctcgcc aaaaaacaac cttgttcgtt
      961 tactctattc tcatttaccc tcacatcagt ctgatgagat atcatatatt ctcttggtat
     1021 agccccctgc aataaaaccc catataacat aacacctgta tgaatacatc cccgtttgca
     1081 aggcttccgt tgttccgaca tcgacgagta attgcagcac agtattgcat ccagcaatgc
     1141 gaactactat gagaattagc tgaccgggtc cttattcccc gaacgaactt taaatccaaa
     1201 acctcatact cttttccgtc gtcatcacgt ccgtcaccat aacaaccttt cagcatccac
     1261 ccttttatta agagttaatg tccatcagca tcagcaatta ataccgcctg atactgacat
     1321 atttttgtcg tatttacgtc cataatcggt tattgtgata caggtcacac cagcttattt
     1381 gctattgtta caataaccac cttggttgag agctgttttt tcaactttgt tatcaactcc
     1441 cccaactcct tggtcagtaa ggctgccgac cgagcacagt gaatagatag tatccgcagg
     1501 taccaccatg cttacttaat actcagaagg cgaagtgctc tccaattaac tctgccgcac
     1561 tatgcttata agctgctttt caatgccgtc tatcaacatg tccaattcat tcaatctgtt
     1621 gtactggtaa tccaacgcag taatgatata tgcaggtatt ctttcatcca ctccagtaac
     1681 gtttcatgca aagcattaat ttacgcgcta cggaacttca ccagttgact gagcaaacga
     1741 tgtgggacca gtactgcctg cagttcttct attgttacag cgatttcttt accaaacagc
     1801 tgaaccacca tcaagataaa gatagcctga acattcatcg caccattttg ttaccaatgg
     1861 caaaagattt aacgaaccgt gcctgcatca ggcggacttt ttatccctgc tttgttagct
     1921 ccctaacctt gtacagagag cctccataag ctcccatatc aagcagacat ggttcgcagt
     1981 tactgacata caccagaaaa ctctgacagc agactcactt atcctccact tcactcatat
     2041 ccttattggt gagatgaacc tgaatgacat gttttgcgac ataaacgcca accagtgtat
     2101 atttcacctg tagagattct acaatctgta gcgttatgca tctaatgtta ggctctatga
     2161 tgtcagaaat ctgcgagact ctacaccttc tgcatttacc tttatttatc accatgctcc
     2221 tcagttaacg gagaacgtgt tcattgcatt ttgtcactta catttcgcct ttggtatcgg
     2281 ggggatatag gttccaacgt tccgcatgag aacctgaacc ggcttcacgc caccttgata
     2341 ccagatgccg cacaggtagt caacaagtgt cacgaacggt gcaatagtga tccacaccca
     2401 acgcctgaaa tcagatccag ggggtaatct gctctcctga ttcaggagag cttatggtca
     2461 cttttgagac agttatggaa attaaaatcc tgcacaagca gggaatgagt agccggacga
     2521 ttgccagaga actggggctc tcccgcaata ccgttaaacg ttatttgcag gcaaaatctg
     2581 agccgccaaa atatacgccg cgacctgctg ttgcttcact cctggatgaa taccgggatt
     2641 atattcgtca acgcatcgcc gatgctcatc cttacaaaat cccggcaacg gtaatcgctc
     2701 g
//
LOCUS       DAZPAB010000322         2692 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZPAB010000322 DAZPAB010000000
VERSION     DAZPAB010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.324"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..893
                     /locus_tag="ACHUPG_004090"
     CDS             <1..893
                     /locus_tag="ACHUPG_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFR0863459.1"
                     /translation="ELARVGVNPHDVAQMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQ
                     DKMQGRQVTMRGQDLDSQTAARNQAITMRGQDIQANLGQQRINLDAETNRINNENKRL
                     DRMLSAETNDLKRQEIQSRIAANNQQLQQKQQALNDGYKDGINTLTTSMFTLNDIVSS
                     PSLKSITGLRGVIPNVPGSQAADTQARLDTFKSQAYLTAVQAMRGMGALSDAEGKKLD
                     QAVGSLQNSQSEESFRRNAGVILNTLNQKRNEAVGKYVQQNGIKRVEAPQASIDYLKQ
                     HPELSIDFINRYGYLPSLGQ"
     gene            894..2624
                     /locus_tag="ACHUPG_004091"
     CDS             894..2624
                     /locus_tag="ACHUPG_004091"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR0863460.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEKRVEVA"
BASE COUNT          757 a          661 c          707 g          567 t
ORIGIN      
        1 gtgagttagc tcgtgttggc gttaatcctc atgacgtcgc tcagatgtac caacagaacc
       61 cgcggcagtt cggcgaattt gtcgatcacc tggggatgaa cagtctcggg cccgaaaaat
      121 actttgacct acaggataaa atgcagggtc gccaggttac catgcgcggt caggatctgg
      181 attcgcaaac cgccgctcgg aatcaggcaa tcacaatgcg cggacaagat atccaggcga
      241 atttaggtca gcagcgcatt aatctggacg cagaaacaaa ccgcattaac aacgaaaata
      301 agcgccttga ccggatgcta tcagcagaaa ctaacgacct gaagcgccag gaaatacaga
      361 gccgcatagc agccaacaac cagcagttgc agcagaagca gcaagcgcta aatgatggct
      421 acaaagacgg catcaacacc ctcacaacca gcatgttcac tctgaacgat atcgttagtt
      481 ctccttcact taagagcatt acaggcttac gtggagtaat ccccaacgtt ccaggctcac
      541 aggctgcaga cactcaggca cgacttgata cctttaaatc acaagcatac ctgacagcgg
      601 ttcaggccat gcgaggcatg ggcgcacttt ctgatgccga gggcaaaaag ctcgaccagg
      661 ctgttggttc gctgcagaac tcgcagagcg aggagtcctt tcgtcgcaac gctggcgtca
      721 tcctgaacac gctcaaccag aagcgtaatg aggcggttgg taagtacgtt cagcaaaacg
      781 gtatcaagcg agtggaagcg cctcaggctt ctatagatta cctgaagcag caccccgagc
      841 tgtcaatcga cttcattaat cgctacggat atcttccatc tttggggcag taaatggcta
      901 attaccgtga tttgttagag caggctggcg cacgttacgg tgtgccagaa gggttgatga
      961 ctgcactggg tgccaaggag tcttcttaca accctgccgc agtaagctcc gccggggctg
     1021 taggattgac tcaggtcatg cctgggacat ggcgtgatat gggttatacc gatgagcaaa
     1081 tgcaaaaccc cgaatatcag gctgacgctg gcgcgcgcta tctggcaaag atgtaccagc
     1141 agtttggtaa ctggcgtgac tctcttcagg cttatcacga cggtcccggc aacgttatga
     1201 aggcaaagcg tggtgaatat acgccaggac ctgaaggccg cggttacgtt gatgatcgct
     1261 ttgctcaatg ggctggtgac ccggtgacag actcaacagt cgaacagcgc gccacctctg
     1321 caaaggtaca tcctcagcaa gaccctaaca acccgtttgc acaactggaa gtacagtcat
     1381 ccgaacaagt atcggcatca ggtgtgcagt cagacccaaa taatccattt gctcagattg
     1441 agcagcaggc agccagtcag cagccacctc aacccgtaag ttctgtcgca ccgaaacctg
     1501 ttcagcaaca aacagttaat caggccaata atgcaccaac acgggaagaa ccatcattga
     1561 tgcaacaagc tggcgattgg ctcactggtg gtcaaagtgc agggcaaatt gcagaacagg
     1621 ctggtcgtgg tctggtaaac ataccatttg acgtattgca gggtggcgca agtctgatta
     1681 atgcaatcag tcaggggctt ggggggccaa aagtattgga tgatgtttat cgtccagtag
     1741 acagaccgac agacccctac gcgcaagctg gagagtcaat aggcggttat cttgttcctg
     1801 gagcaggtgt agctggaaac atggtcattg gttctctcgc tgacgcggcg aatcaacggg
     1861 gtgattttgc cgaaaatgcc gctattaatg ccggacttag cattgctacg catggcctga
     1921 taaatggcgt tacccgtggt gttcgtggtg catcaaatat aattagtggc aataaaacat
     1981 ctgcacagag agcgaccact gcgccaacag aaacatcacc attctccggt gatgccgctg
     2041 cagcaacaaa tcctgcggtt catgccgcag aggcaagagt agcacaaggt gtaccaatga
     2101 cgcctgcgac gaagaaccca gaggaagtcg ttcgcacagt agcagcacaa aaaaggccaa
     2161 atctcgcttc atcgcttgat gaactagata tcaatcctca ggctgaagtt ctggagtctg
     2221 ctgaaaggct taatgttgat tcattactcc cttcacactt ttccgggaac gagcaataca
     2281 aggcagttga gcaagcaatc aagtcccgtg cgggttctgc tctacaggtg caggaaaatg
     2341 aagcaatcag gcagctagca cagggcgcgg gtgagataat tgatcgcgtt tccggtgcaa
     2401 aagatgctct tggtatgagc gacaagttta ttgatacggt caatggaaga atgtctgcgc
     2461 tgatgaaacg aagcgaccag ctttatcgca atgttgaaaa ggcgatgcct gcaggtgcaa
     2521 aaattgatgc gccatcaaca aggtcaatgc tcaaacaggt ggcagaagat cttggcggga
     2581 tgaaaaacct tgaccctatt gaaaagagag ttgaggtagc ctgagtttaa cggacactcc
     2641 ttcctgaaat agaatggcat cagaaggagc taataatgag cagaaaaacc ca
//
LOCUS       DAZPAB010000323         2680 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZPAB010000323 DAZPAB010000000
VERSION     DAZPAB010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.326"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            169..1272
                     /gene="proB"
                     /locus_tag="ACHUPG_004092"
     CDS             169..1272
                     /gene="proB"
                     /locus_tag="ACHUPG_004092"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR0863461.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1284..2537
                     /gene="proA"
                     /locus_tag="ACHUPG_004093"
     CDS             1284..2537
                     /gene="proA"
                     /locus_tag="ACHUPG_004093"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR0863462.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
BASE COUNT          637 a          678 c          769 g          596 t
ORIGIN      
        1 gtggtaattt attaaatctg taataaaagc gtaaacaact gccgctacgc ttgctgatcc
       61 cgcgcaacaa aacgccatgc tttgctcgca gatggttggc aaccgacgac agtcctgcta
      121 aaacgttcgt ttgatatcat ttttcctaaa attgaatggc agagaatcat gagtgacagc
      181 cagacgctgg tggtaaaact cggcaccagt gtgctaacag gcggatcgcg ccgtctgaac
      241 cgtgcccata tcgttgaact tgttcgccag tgcgcgcagt tacatgccgc cgggcatcgg
      301 attgttattg tgacgtcggg cgcgatcgcc gccggacgtg agcacctggg ttacccggaa
      361 ctgccagcga ctatcgcctc gaaacaactg ctggcggcgg tagggcagag tcgactgatt
      421 caactgtggg aacagctgtt ttcgatttat ggcattcacg tcgggcaaat gctgctgacc
      481 cgtgctgata tggaagaccg tgaacgcttc ctgaacgccc gcgacaccct gcgtgcgttg
      541 ctcgataaca atatcgttcc ggtaatcaat gagaacgatg ctgtcgctac ggcagagatt
      601 aaggtcggcg ataacgataa cctttctgcg ctggcggcga ttctggcggg tgccgataaa
      661 ctgttgctgc tgaccgatca aaaaggtttg tataccgctg atccgcgcag aaatccgcag
      721 gcagaactga ttaaagatgt ttacggcatt gatgacgcac tgcgcgcgat tgccggtgac
      781 agcgtttcag gcctcggaac tggcggcatg agtactaaat tgcaggccgc tgacgtggct
      841 tgccgtgcgg gtatcgacac cattattgcc gcgggcagca agccgggcgt tattggtgat
      901 gtgatggaag gcatttccgt cggtacgctg ttccatgccc aggcgactcc gcttgaaaac
      961 cgtaaacgct ggattttcgg tgcgccgccg gcgggtgaaa tcacggtaga tgaaggggca
     1021 actgccgcca ttctggaacg cggcagctcc ctgttgccga aaggcattaa aagcgtgact
     1081 ggcaatttct cgcgtggtga agtcatccgc atttgcaacc tcgaaggtcg cgatatcgcc
     1141 cacggtgtca gtcgttacaa cagcgatgca ttacgccgta ttgccggaca ccactcgcaa
     1201 gaaattgatg caatactggg atatgaatac ggcccggttg ccgttcaccg tgatgacatg
     1261 attacccgtt aaggagctgg ccgatgctgg aacaaatggg cattgccgcg aagcaagcct
     1321 cgtataaatt agcgcaactc tccagccgcg aaaaaaatcg cgtgctggaa aaaatcgccg
     1381 atgaactgga agcacaaagc gaaatcatcc tcaacgctaa cgcccaggat gttgctgacg
     1441 cgcgagccaa tggccttagc gaagcgatgc ttgaccgtct ggcactgacg cccgcacggc
     1501 tgaaaggcat tgccgacgat gtacgtcagg tgtgcaacct cgccgatccg gtggggcagg
     1561 taatcgatgg cggcgtactg gacagcggcc tgcgtcttga gcgtcgtcgc gtaccgctgg
     1621 gggttattgg cgtgatttat gaagcgcgcc cgaacgtgac ggttgatgtc gcttcgctgt
     1681 gcctgaaaac cggtaatgcg gtgatcctgc gtggtggtaa agaaacctgt cgcactaacg
     1741 cggcaacggt ggcggtgatt caggacgccc tgaaatcctg tggcttaccg gcgggtgccg
     1801 tgcaggcgat tgataatcct gaccgtgcgc tggtcagtga aatgctgcgt atggataaat
     1861 acatcgacat gctgatcccg cgtggtggcg ctggtttgca taaactgtgc cgtgaacagt
     1921 cgacaatccc ggtgatcaca ggtggtatag gcgtatgcca tatttacgtt gatgaaagtg
     1981 cagagatcgc tgaagcatta aaagtgatcg tcaacgcgaa aactcagcgt ccgagcacat
     2041 gtaatacggt tgaaacgttg ctggtgaata aaaacatcgc cgatagcttc ctgcccgcat
     2101 taagcaaaca aatggcggaa agcggcgtga cattacacgc agatgcagct gcgctgacac
     2161 agttgcaggc aggccctgcg aaggtggtgg cggttaaagc cgaagagtat gacgatgagt
     2221 ttctgtcatt agatttgaac gtcaaaatcg tcagcgatct tgacgatgcc atcgcccata
     2281 ttcgtgaaca cggcacacaa cactccgatg cgatcctgac ccgcgatatg cgcaacgccc
     2341 agcgttttgt taacgaagtg gattcgtccg ctgtttacgt taacgcctct acgcgtttta
     2401 ccgacggcgg ccagtttggt ctgggtgcgg aagtggcggt aagcacacaa aaactccacg
     2461 cgcgtggccc aatggggctg gaagcactga ccacttacaa gtggatcggc attggtgatt
     2521 acaccattcg tgcgtaaata aaaccgggtg atgcaaaagt agccatttga ttcacaaggc
     2581 cattgacgca tcgcccggtt agttttaacc ttgtccaccg tgattcacgt tcgtgaacat
     2641 gtcctttcag ggccgatata gctcagttgg tagagcagcg
//
LOCUS       DAZPAB010000324         2657 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZPAB010000324 DAZPAB010000000
VERSION     DAZPAB010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.327"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(1..633)
                     /gene="mxiE"
                     /locus_tag="ACHUPG_004094"
     CDS             complement(1..633)
                     /gene="mxiE"
                     /locus_tag="ACHUPG_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HFR0863463.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            complement(763..1191)
                     /gene="mxiM"
                     /locus_tag="ACHUPG_004095"
     CDS             complement(763..1191)
                     /gene="mxiM"
                     /locus_tag="ACHUPG_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HFR0863464.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            complement(1175..1582)
                     /gene="mxiM"
                     /locus_tag="ACHUPG_004096"
     CDS             complement(1175..1582)
                     /gene="mxiM"
                     /locus_tag="ACHUPG_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HFR0863465.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            complement(1575..2270)
                     /gene="mxiN"
                     /locus_tag="ACHUPG_004097"
     CDS             complement(1575..2270)
                     /gene="mxiN"
                     /locus_tag="ACHUPG_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HFR0863466.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            complement(2212..>2657)
                     /locus_tag="ACHUPG_004098"
     CDS             complement(2212..>2657)
                     /locus_tag="ACHUPG_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFR0863467.1"
                     /translation="LPSELLENGVIRSEINNLIINKYDLNCDIEPLSGVTAMFVANWN
                     LLPAVAYFIGSQESRLINHSEMVISYYGGKISKQGEAAIRSGFWHLIAWKENISVGIY
                     ERINLLFNPIALEGNYTPVERNLSRLNEGMQYAKRHFTGIQTSCL"
BASE COUNT          856 a          524 c          327 g          950 t
ORIGIN      
        1 ttaaattttt tcatttattt ttttcactaa aaaagtaata ttcgataatt ctcttgcaga
       61 aaagcctagt cgtgttttta tctcatttga aaaatgggat gttgatgcat acccattatt
      121 catggcagct gaagtgattg tttgattgtg caaaaataca tctaataaac catttactaa
      181 ccgccaagtg ttcagttgtt ctttgacttt tgcccctaaa gcttttctac ataaagatct
      241 aaaataagcc tccgacacac cataatgctc tgtcagactc tttattctta cctccttttc
      301 tttctcaata gttctcagaa gataaagaac caaatgatat gactcagtca ttcgtatcat
      361 agccagtgca ttacttacat tctggtcaga tgaaaagatg aatcttaaca ttaacttatc
      421 aagcatcgat tcggtattac aactatcccg aagatcattc tcatgataga aatgaataca
      481 tttttcagca tggctatgct ctactagagc accactgaaa tagtcccaaa aagcaagaaa
      541 ttttgcacaa ctattaatgt taaagcgata atgatagttc acattatcac tgaattttat
      601 attttgattt cggacaaaaa aaaacccttc catgctggag ccagctcaat tatgttacgt
      661 tctttatctt ttacaagctc aacgttaact ggttcatgtc cactagagta aatgtaaaac
      721 atattacttg tatttagacc tttatattta ctcacaatac aattatatcc ctcgaattaa
      781 ttttaattta acttccccat ccttctctat aaggatggta tcatttatct tattaccttt
      841 taaaacaacc ccgtctttca gcatagaaaa tagtttatct atagctatct tttgtgcatc
      901 catgaagcac ccatctactt ccttaacatt aaagttgccc atgaatttac ctgcattaaa
      961 actataaacc gccttaccac taatacattt agagtaaaca tttatacttt tattggttgt
     1021 attaaacgac cataataaat catttggaat agaaaaataa tccttactta caggaacaat
     1081 atgccattct ttttcagaat tagatgatga ctttaaagca cacccactca gtgttaatag
     1141 caatatactt aaaataaata ttttcaactt attactacca tgtcgaatca tctgcctctc
     1201 tccctgcttc aaagacacta attaatgaat cgaaggttgt agtcttaata tagaaatcaa
     1261 tattgttata tatggacaat gatgcataaa atccctcatg caaatcgaca tattgatttg
     1321 catctatgag tttattaaga agtgaacttt tttgtgcagc tgtaagataa ggaaaacgtg
     1381 aattgaataa ttgttcaatc ttattgtgca gttcatcatt atctatggaa tttaattcac
     1441 tctgtgagaa atattgcatt atttcataga ttatatcctc atcaaatgat tgcgaagatg
     1501 ataatctctc atttaaatta acaacttctt cactatttaa tctttgctgc agagcattgc
     1561 ttgcatttat ttgattaatc attaacagga ttctctttta acaagtcttc tgcaatttgt
     1621 ctcagtttct tatgtgctaa ctctttgaaa tattttttat cattttttgt taatttttcc
     1681 gcaagttcta ttttaacacc tgatattaca tcctgaggac taaactccac tacttgatta
     1741 ccactacaga atatataatt attcccttca gaatatttta aaataatctt gacatctgag
     1801 cgatatttat ggagatcaag ttctaacttt ttcctcagtg ctaaattaca cttgggcaaa
     1861 accactttta attctgttac tgtattacgc aacgaactaa gccactgctc caataaagtg
     1921 ctaatgatcc tctcatcatg taaattttct gaaagcaaac tagttatata gtcctcaata
     1981 tttcttctat tttcattttg ctttaacttc cattcacaaa tatagtcaat aatatgctca
     2041 aaagcagtct gtataccaat ttgataacca tcacatacag catctatacg cagtgattca
     2101 gcttttttcg tagcctcctt tataacacaa attgctttct tcttactctc agtgtctcta
     2161 tcttttatgg ttttacataa ttccttttca attctcttta taacaacacc atcataggca
     2221 tgatgtctgg ataccggtaa agtgcctttt tgcatattgc ataccttcat ttaatcggga
     2281 caaatttctt tctacaggcg tataatttcc ctccaaagca attggattaa ataataagtt
     2341 tattctttca tatataccaa cagatatatt ttccttccac gcaataagat gccaaaaacc
     2401 acttcgaata gcggcctccc cttgcttact gattttgccc ccataataac taataaccat
     2461 ctcactatga ttaattaacc gagactcttg actaccgata aaataagcaa cagctggaag
     2521 taaattccag ttggcaacaa acatagcggt taccccgctt aaaggttcaa tatcgcaatt
     2581 tagatcatat ttattaatta tgagattatt aatctcactt ctgattacgc cattttctaa
     2641 cagttcagaa ggcaaat
//
LOCUS       DAZPAB010000325         2616 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZPAB010000325 DAZPAB010000000
VERSION     DAZPAB010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.328"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            100..1065
                     /gene="dusB"
                     /locus_tag="ACHUPG_004099"
     CDS             100..1065
                     /gene="dusB"
                     /locus_tag="ACHUPG_004099"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR0863468.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1091..1387
                     /gene="fis"
                     /locus_tag="ACHUPG_004100"
     CDS             1091..1387
                     /gene="fis"
                     /locus_tag="ACHUPG_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR0863469.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1473..2357
                     /gene="yhdJ"
                     /locus_tag="ACHUPG_004101"
     CDS             1473..2357
                     /gene="yhdJ"
                     /locus_tag="ACHUPG_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR0863470.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            2441..>2616
                     /locus_tag="ACHUPG_004102"
     CDS             2441..>2616
                     /locus_tag="ACHUPG_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR0863471.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
BASE COUNT          723 a          614 c          645 g          634 t
ORIGIN      
        1 tccatctcag aggattggtc aaagtttggc ctttcatctc gtgcaaaaaa tgcgtaatat
       61 acgccgcctt gcagtcacag tatggtcatt tcttaactca tgcgcatcgg acaatatcag
      121 ctcagaaatc gcctgatcgc agcgcccatg gctggcatta cagacagacc ttttcggacg
      181 ttgtgctacg agatgggagc cggattgaca gtatccgaga tgatgtcttc taacccacag
      241 gtttgggaaa gcgacaaatc tcgtttacgg atggtgcaca ttgatgaacc cggtattcgc
      301 accgtgcaaa ttgctggtag cgctccgaaa gaaatggcag atgcagcacg tattaacgtg
      361 gaaagcggtg cccagattat tgatatcaat atgggttgcc cggctaaaaa agtgaatcgc
      421 aagctcgcag gttcagccct cttgcagtac ccggatgtcg ttaaatcgat ccttaccgag
      481 gtcgtcaatg cagtggacgt tcctgttacc ctgaagattc gcaccggctg ggcaccggaa
      541 caccgtaact gcgaagagat tgcccaactg gctgaagact gtggcattca ggctctgacc
      601 attcatggcc gtacacgcgc ctgtttgttc aatggagaag ctgagtacga cagtattcgg
      661 gcagttaagc agaaagtttc cattccggtt atcgcgaatg gcgacattac tgacccgctt
      721 aaagccagag ctgtgctcga ctatacaggg gcggatgccc tgatgatagg ccgcgcagct
      781 cagggaagac cctggatctt tcgggaaatc cagcattatc tggacactgg ggagttgctg
      841 cccccactgc ctttggcaga ggttaagcgc ttgctttgcg cgcacgttcg ggaactgcat
      901 gacttttatg gtccggcaaa agggtaccga attgcacgta aacacgtttc ctggtatctc
      961 caggaacacg ctccaaatga ccagtttcgg cgcacattca acgccattga ggatgccagc
     1021 gaacagctgg aggcgttgga ggcatacttc gaaaattttg cgtaaacaga aataaagagc
     1081 tgacagaact atgttcgaac aacgcgtaaa ttctgacgta ctgaccgttt ctaccgttaa
     1141 ctctcaggat caggtaaccc aaaaacccct gcgtgactcg gttaaacagg cactgaagaa
     1201 ctattttgct caactgaatg gtcaggatgt gaatgacctc tatgagctgg tactggctga
     1261 agtagaacag cccctgttgg acatggtgat gcaatacacc cgtggtaacc agacccgtgc
     1321 tgcgctgatg atgggcatca accgtggtac gctgcgtaaa aaattgaaaa aatacggcat
     1381 gaactaattc aggttagcta aatgcttgat taaaaaggcg ctactcggca tggggaagcg
     1441 ccttttttat aggtgtcaca aagggagtga ccatgagaac aggatgtgaa ccgacccggt
     1501 ttggtaatga agctaagacc attattcacg gtgatgcctt tgccgaactt aaaaagctac
     1561 ctactgaaag cgtcgatctg ctctttgccg acccaccgta taacatcggt aaaaattttg
     1621 atggtctgat cgaagcctgg aaagaagatc tgtttatcga ctggctgttt gaagtgattg
     1681 cagagtgcca ccgcgttctg aaaaagcagg gcagcatgta cattatgaac agtacggaaa
     1741 acatgccctt tatcgatctc cagtgccgca agctttttac catcaaaagt cgcatcgtct
     1801 ggtcatatga cagttctgga gtacaggcga aaaaacacta cggctccatg tacgaaccca
     1861 tcctgatgat ggtgaaagac gcaaagaact acacattcaa cggtgatgct attctggtag
     1921 aagccaaaac cggatcgcag cgcgcgttga tcgattatcg caaaaatcct ccacagccat
     1981 acaatcatca aaaagtaccg ggtaacgtct gggattttcc gcgcgtgcgt tatttgatgg
     2041 atgaatatga gaaccacccg acgcaaaaac cgaaagcctt actgaaacgc attattctcg
     2101 cctcttccaa cccaggcgat atcgttctcg acccgtttgc cggtagcttt actaccggtg
     2161 ccgtagccat cgccagcgga cgaaaattca ttggtattga gatcaacagc gagtacatca
     2221 aaatggggct tcgacggctg gatgtcgcgt cgcattactc cgcggaagaa ctggcgaaag
     2281 tgaaaaaaag aaagaccggc aacctgtcaa aacgaagccg gttaagcgaa gttgaccccg
     2341 atctcattgc aaagtaaagg aagtgtaagc ctagttttca gattattcat ttcgtgtata
     2401 tttcctgcca gacttggtta aacatgcaca ggctcaggta atgattcgca agtattggtg
     2461 gctcgtcgtt ttcgctgtct tcgtttttct gtttgatact ttactgatgc agtggattga
     2521 actgctgaca acagaaacag acaaatgccg taatatgaac tcagttaatc cactaaaact
     2581 ggttaactgt gacgaactga attttcagga cagaat
//
LOCUS       DAZPAB010000326         2556 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZPAB010000326 DAZPAB010000000
VERSION     DAZPAB010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.329"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..823)
                     /locus_tag="ACHUPG_004103"
     CDS             complement(<1..823)
                     /locus_tag="ACHUPG_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0863472.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1422..1670)
                     /locus_tag="ACHUPG_004104"
     CDS             complement(1422..1670)
                     /locus_tag="ACHUPG_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR0863473.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(1933..2028)
                     /locus_tag="ACHUPG_004105"
                     /pseudo
     CDS             complement(1933..2028)
                     /locus_tag="ACHUPG_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2025..2372)
                     /locus_tag="ACHUPG_004106"
                     /pseudo
     CDS             complement(2025..2372)
                     /locus_tag="ACHUPG_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
     gene            complement(2399..>2556)
                     /locus_tag="ACHUPG_004107"
     CDS             complement(2399..>2556)
                     /locus_tag="ACHUPG_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR0863474.1"
                     /translation="ALRDPLSRAYYTRKMSQGKRHNQVLIALARRRCDVLFAMMRDGT
                     FYTPQGS"
BASE COUNT          649 a          486 c          628 g          793 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggcaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggtta tttttccatt
      721 cagtccaatg ggttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggtgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggggggc aatttttcct taaggtatga aaaaaggagc
     1081 tgaagctata taatagctat taatgccact ggtatcagtg aatcatgtat ataaaaaact
     1141 gtgttaagga ggctgtccat tagcggtttt ttattcttct ggaaccgcgg catctttgcg
     1201 tatctttttg tcttctcacc gtctggtcgg tgttttgctg agactgctaa tttcctgtct
     1261 ttgctagtgc tgtactggta ctgcccaatc atgattggcg ggggcgggcg gagttgagac
     1321 cgcagacacg tcgtatgcaa gaacgtgctg cggctggctg gtgaacttcc gatagtgcga
     1381 gtattgaatg atttccagcc gttactgatt ttacgtgcta attagtgaac aaaccactcg
     1441 tcagcagact cccaggtatc tttcagagtt tcctgaacaa aagttttagc tgaatcttta
     1501 tcggcggtgc gcgtaacaga aaggccatcg ttgctggtgg cttttacgat cacctctaca
     1561 tcgtcataac gtttactgat gcgtcgggtt aattcttcct ttaacgcatc cacagcaccg
     1621 gttggcattt tagtcatttt ctctttggct atgcagattt caaaacgcat aaaaatccct
     1681 ctgcactgtg tttttataca gtattatttt taactgtatg gataaacagt gtcaagagtg
     1741 agggaaagtg attttttggg gcatgcatgg ggcaaaaata tttgttgtgg ggcattttgg
     1801 ggcatgattg ggacactttg ttacatatga actttgccga ttttcatatg acatggcaat
     1861 ccaacgcttt gattattcta aagaaaacac atgctcttgg gcgttcttta gtgattttta
     1921 aaaatcccgt atcacgcagt taaagtggcg ggcatactct tcaaggctgg tgatccccaa
     1981 ccgcacccat ttaggatgtg accattgcgg tagaccgatg taaatcataa cgcgtgaagg
     2041 atctcttcca cgctacgcac acgggcgatg cgcgggtaga tatgattaat gctgttattg
     2101 tgttgctcgg cgctggcggc actacaggcg tcttcggcaa tcaccagatt aaagccaagt
     2161 tcccaggcat tgcgggcggt ggattcaaca ccgatattgg tcgagatccc acataacact
     2221 attgtatcga tacctcggcg gcgcagttgc aactccagat ccgtaccgta aaacgcaccc
     2281 cactgacgtt tgatgatttc gatatcactg tcagttgcac ctaatgcagc tggatgttgc
     2341 caccagtttt cgggcaacac tttagcgggg gagtgcccct attaagttgt caagcatgtt
     2401 atgacccctg cggggtataa aaagtcccgt cgcgcatcat ggcgaacaga acgtcgcagc
     2461 gtcgtctcgc cagggcgata agcacctgat tgtgtcgttt tccctgactc attttgcggg
     2521 tgtagtaagc cctggagagc ggatccctga gcgcgg
//
LOCUS       DAZPAB010000327         2525 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZPAB010000327 DAZPAB010000000
VERSION     DAZPAB010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.330"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..197
                     /locus_tag="ACHUPG_004108"
     CDS             <1..197
                     /locus_tag="ACHUPG_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR0863475.1"
                     /translation="LVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQ
                     RGVNVFIQDVPGDQKEQIPD"
     gene            236..1039
                     /gene="agaC"
                     /locus_tag="ACHUPG_004109"
     CDS             236..1039
                     /gene="agaC"
                     /locus_tag="ACHUPG_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFR0863476.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1029..1820
                     /gene="agaD"
                     /locus_tag="ACHUPG_004110"
     CDS             1029..1820
                     /gene="agaD"
                     /locus_tag="ACHUPG_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFR0863477.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1821..>2525
                     /locus_tag="ACHUPG_004111"
     CDS             1821..>2525
                     /locus_tag="ACHUPG_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFR0863478.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIP"
BASE COUNT          603 a          601 c          654 g          667 t
ORIGIN      
        1 aattggtaga aggtggcatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg
       61 aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc gcggacttac
      121 gttttattaa acaacgtggc gtgaatgttt tcattcagga cgtccctggc gatcaaaaag
      181 aacaaatccc tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa taaaaatgca
      241 tgaaataacc ctacttcagg gattatccct agcggcgtta gttttcgttc tggggattga
      301 tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc taactggcgc
      361 tattctcggt gatattcaga ctggcttaat taccggtggt ctgacagagt tggctttcgc
      421 cggattaacc cctgcaggtg gtgttcagcc gcccaacccg attatggcgg gtctgatgac
      481 caccgtcatt gcatggtcta cgggcgttga tgccaaaaca gcaattggtc ttggcctgcc
      541 gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct cattatttat
      601 gaccaaagcc gataaatgcg cgaaagaggc ggatacggca gcgttttccc ggcttaactg
      661 gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct gtacttacct
      721 ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggttga cccacggctt
      781 tgaagtggct ggcggtattc tgcctgccgt tggttttggc ttgctgctgc gcgtaatgtt
      841 caaagcgcaa tatatccctt acctgatcgc cggtttcctg tttgtttgct acatccgggt
      901 cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg agtttttcaa
      961 tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg aagaagagga
     1021 ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc tttcgttcgt
     1081 cgctgctgca agcgagcttt aactacgaaa ggatgcaggc gggcggtttt acctgggcga
     1141 tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcttaagc gcggcgatga
     1201 aagataacct cgaatttatt aatacccacc cgaatctggt cggattcctg atggggttat
     1261 taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc aaagtggcac
     1321 tgtttggccc aatcgccggg attggcgatg cgattttctg gtttactttg ttgccgatta
     1381 tggcgggaat ttgctcatca tttgccagcc agggaaacct gctggggccg attctatttt
     1441 tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc ggttattcag
     1501 tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca gatgattgct cgctcggcaa
     1561 ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat attaacgtgg
     1621 tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct gcaacaggat ttcttcgata
     1681 aagtcttccc gaacatttta ccgatggcct acaccctgct gatgtattac ttcctgcggg
     1741 tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct attgtttgtt
     1801 ccgcattcgg cattttgtaa atggaacgag gcactgcttc tggcggtgcc tctttactta
     1861 aggaatttca tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg ttaagtgaac
     1921 gagccagcga atatttattg gctgtgatcc gtagcaaacc ggatgccgtg atttgcctgg
     1981 cgaccggagc cacgccatta ctgacgtatc attatctggt agaaaaaatc caccagcagc
     2041 aggttgatat cagccagctc accttcgtga agctcgacga atgggtggat ctgccattaa
     2101 cgatgcctgg cacctgcgaa actttcctac aacagcatat cgtgcagccg ctggggctac
     2161 gtgaagacca gctcatcagc tttcgctccg aagagataaa tgagacagag tgcgaacggg
     2221 taacgaacct gattgcgcgc aaaggcggtc tggatttatg cgttctcgga ttggggaaaa
     2281 acggtcatct tgggctgaac gaaccgggag aaagcctaca accggcctgc catatcagtc
     2341 aacttgatgc cagaacacag caacatgaga tgttaaaaac cacgggtcgc cccgtgactc
     2401 gtgggatcac cttaggcctg aaggatattc tcaatgcccg cgaggttttg ttattggtga
     2461 ctggcgaagg aaagcaggat gcgacagaac gttttctcac ggctaaagtc tccaccgcta
     2521 tcccg
//
LOCUS       DAZPAB010000328         2513 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZPAB010000328 DAZPAB010000000
VERSION     DAZPAB010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.331"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..567
                     /locus_tag="ACHUPG_004112"
     CDS             <1..567
                     /locus_tag="ACHUPG_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0863479.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            525..1202
                     /gene="bglJ"
                     /locus_tag="ACHUPG_004113"
     CDS             525..1202
                     /gene="bglJ"
                     /locus_tag="ACHUPG_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR0863480.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(1240..2028)
                     /gene="fhuF"
                     /locus_tag="ACHUPG_004114"
     CDS             complement(1240..2028)
                     /gene="fhuF"
                     /locus_tag="ACHUPG_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR0863481.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2169..2405
                     /locus_tag="ACHUPG_004115"
     CDS             2169..2405
                     /locus_tag="ACHUPG_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR0863482.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          645 a          555 c          649 g          664 t
ORIGIN      
        1 ttagtcattg ctgatttagc cggtgaaagt gaagatccac gttctgtttg tgaacattat
       61 tattctttaa tctcacaata tcgggaaatt cactgggttt tcatggtgtc acgctcctgg
      121 tattcccagg ctgtagaact gctcatgtgc cctacggcga cgttattgtc tgatgttgaa
      181 cccattgaga atctggtcaa gaccgtacgt tccggcaata cgcacgcaga gcgtatcagc
      241 gccatgctga cctctccggc aatgactgaa actcatgatt ttagctatcg ctccgtcatt
      301 ctcactcttt cagagcgcaa ggtactgcgg ctattaggta aaggatgggg catcaaccag
      361 atagcttcat tgcttaagaa aagtaataaa actatcagcg cccaaaaaaa cagtgcgatg
      421 cgtcgactgg caattcacag caacgctgaa atgtatgcat ggataaatag cgcgcagggt
      481 gcaagagaac ttaacttgcc ttctgtttat ggagatgccg cagaatggaa cacagccgaa
      541 ttaagaagag aaatgtcgca ctcatagaaa aatgcgtcat gagtagtatc ggtattgaga
      601 gtttattcag aaagtttgcg ggcaaccctt ataagctcca tacctatacc agtcaggagt
      661 catttcagga tgccatgtcg cggatctcgt ttgcggcggt cattttttct ttttctgcca
      721 tgagaagtga gcgcagagag ggattatctt gcctgactga gctggcgatt aagtttccgc
      781 gtacccggcg tttagttatt gtggatgatg atattgaagc tcggctgatt ggttcattgt
      841 cgccatcacc gctggacggt gtattaagta aagcgtcaac gctggagatt tttcatcagg
      901 aactcttttt gtcattaaat ggtgtacgtc aggcgaccga ccgactgaac aatcagtggt
      961 acattaacca aagccggacg ttaagcccga cggagagaga aatattgcgc tttatgtcgc
     1021 gtggctactc aatgacacaa attgccgagc agcttaaacg taatatcaaa acgatccgag
     1081 cgcataaatt taatgtgatg tcgaaactgg gcgtcagttc tgatgcaggg ttgttggagg
     1141 ccgaagatat tctgttatgt atgcggcatt gcgaagcaag taatgtgttg catccctatt
     1201 aatccgcatg atgccgggtt tacttccccg gcagtgcttt catttcagcg tacaatagcc
     1261 acattgctgc acatccggta agcgataacg ctggcagcaa gtgcggcgca ccagcaggcc
     1321 gtcgcgcagt accacggtac gccagagtgg attatcttca ccgttcgtga gcgttttctc
     1381 aaaaaagagg gcatggcgca gcgattcaac agtagcctcg ccgagcagtt gcttcatctc
     1441 agtgagatac cagttgatca aataaccggt attactccag ataagtttgc cgttgatctc
     1501 tccggtcgct tctagtgctt gcacaaccgg aaccagcgcc tggctgatta acgtttccat
     1561 tcgctgctgc ggcgaatgtg gtgttgcgtt tttatcttca cacacatcga cccagaaaca
     1621 ggcgacgcgt ccggtttcgt gaaactcagc atggaaatgt tccggcgaca catctaatgc
     1681 tttttcctgc gtcagtagcg ccagcattaa tggtggcacc atcaggccga tataccattg
     1741 tgcccatagt gagatcagcg gtttgttctc gcggatcatc gttggttggt tgcgatagat
     1801 atgatcggaa tagaccgcca gtagagaact tagcgcattc ggtgatgacc attgcgccag
     1861 cgtcatggcg ttaagcggag cgggttcatc caggcggata aactccagca aatgttcacg
     1921 atgtttggcg atcatcgccc gtacggcttg tgcaagccca gcatcctgcg gctggagatg
     1981 cgttcgccag atgacatctt catagagcgg tgcggaacga taggccataa tcgtgatagt
     2041 aatctaaatg ataatgattg ctaatcctag cgataggttt actcgatagc aagggatttc
     2101 tctggcttgc aaatgataaa aattatcata tgatattggt tttcattatc aatgaaagag
     2161 atgaaaccat gttgcaacgt acgctgggca gtggctgggg agtgttgctg ccgggattgc
     2221 tgattgcagg gctgatgtat gcggatttat cgccggatca gtggcggatt gtcattctga
     2281 tgggattagt attgacgccg gtgatgctgt atcacaaaca gttgcggcat tacattttgc
     2341 taccatcgtg cctggcgctt attgctggca tcatgctgat gataatgaat ttgaatcagg
     2401 gatgacaaaa cagggaagaa acaagaaagg aagtaaagat aattggtgcg agggggggga
     2461 cttgaacccc cacgtccgta aggacactaa cacctgaagc tagcgcgtct acc
//
LOCUS       DAZPAB010000329         2487 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZPAB010000329 DAZPAB010000000
VERSION     DAZPAB010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.332"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>2487
                     /locus_tag="ACHUPG_004116"
     CDS             <1..>2487
                     /locus_tag="ACHUPG_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR0863483.1"
                     /translation="QEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDV
                     NAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAH
                     QDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIV
                     VGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYY
                     RLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQS
                     LFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERD
                     LRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQM
                     VNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAE
                     NLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGV
                     GELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDN
                     IWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAAD
                     RQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTV
                     KEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSR
                     LSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQNVQRSL
                     DIQQRSVQQLANTIINSLIQYDDPAAWTEQE"
BASE COUNT          728 a          577 c          602 g          580 t
ORIGIN      
        1 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
       61 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
      121 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg
      181 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
      241 gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt
      301 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat
      361 acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg
      421 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg
      481 gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt
      541 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
      601 tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
      661 ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg gagcatgtta
      721 gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt
      781 tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt
      841 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
      901 accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc
      961 acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt
     1021 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
     1081 ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag
     1141 ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa
     1201 aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg
     1261 gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg
     1321 gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt
     1381 gccaaggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tattgccgta
     1441 tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat
     1501 aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa acgctggagt
     1561 gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt
     1621 agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg aatcacccac
     1681 agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa
     1741 acgctcgacc agcgtccagc ggagaaattc gcccagctga tgtatgagac gcgctatgct
     1801 gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc cgcagatgcg
     1861 ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt
     1921 aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggctc aatcaaacac
     1981 tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt
     2041 actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc
     2101 cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca
     2161 aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt ttcttctcgc
     2221 ctctcggttg atcctcaggc taaagatatc agtcatttgc tggcttttac ttgtcaacca
     2281 gaacgacatg atgaacagtt aacgttagcg aatgaagtga tggttaagcg tttgactaag
     2341 gggatcagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag cctcgatatc
     2401 caacagcgta gcgttcaaca attagcgaac actattataa atagtcttat tcaatatgac
     2461 gatcctgcag catggactga gcaggag
//
LOCUS       DAZPAB010000330         2473 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZPAB010000330 DAZPAB010000000
VERSION     DAZPAB010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.333"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            249..392
                     /locus_tag="ACHUPG_004117"
     CDS             249..392
                     /locus_tag="ACHUPG_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR0863484.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            405..614
                     /gene="cspE"
                     /locus_tag="ACHUPG_004118"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             405..614
                     /gene="cspE"
                     /locus_tag="ACHUPG_004118"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR0863485.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            780..1589
                     /gene="rlmA"
                     /locus_tag="ACHUPG_004119"
     CDS             780..1589
                     /gene="rlmA"
                     /locus_tag="ACHUPG_004119"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR0863486.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1586..2152)
                     /gene="mntP"
                     /locus_tag="ACHUPG_004120"
     CDS             complement(1586..2152)
                     /gene="mntP"
                     /locus_tag="ACHUPG_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR0863487.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(2149..2271)
                     /locus_tag="ACHUPG_004121"
     CDS             complement(2149..2271)
                     /locus_tag="ACHUPG_004121"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863488.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
BASE COUNT          652 a          591 c          602 g          628 t
ORIGIN      
        1 ccctctggcc tggaacaact gaacttattg aactatgtta gaaactacgc cagtttaagt
       61 atctgcctga actggcaagg ttaagcacaa tgatatatcg gcgcgtattc cgttgcataa
      121 gtgtgcaaaa aaagtggaag acgtatcgag atttgtgcgt ctgatcgaga tatgtttaaa
      181 aatggcttgc cataattaac gttgtatgtg ataaaagatt tcgggttaaa cgaggtacag
      241 ttctgtttat gtgtggcatt ttcagtaaag aagtcctgag taaacacgtt gacgttgaat
      301 accgcttctc tgccgagcct tatattggtg cctcatgcag taatgtgtca gttttatcta
      361 tgttatgcct gcgggcgaag aaaacaatct aaggaatttt tcaaatggca aagattaaag
      421 gtcaggttaa gtggttcaac gagtctaaag gttttggctt cattactccg gctgatggca
      481 gcaaagatgt gttcgtacac ttctccgcta tccagggtaa tggcttcaaa actctggctg
      541 aaggtcagaa cgttgagttc gaaattcagg acggccagaa aggtccggca gctgttaacg
      601 taacagctat ctgatcgaat ccactgatct gaagtgtgaa tacgcttcaa tctcgctata
      661 aagcctcgtc gaatgcgagg ctttttacta tgctttatct tcgctcctgg cgttcggata
      721 tttgcccgcc gcgtgattcg cgttacactt gcggccttta gtatcctgcc ggagttgtca
      781 tgtcgttttc ctgtccactt tgccatcagc ctctttcgcg tgaaaaaaac agctatatct
      841 gtccccaacg acatcagttt gatatggcga aagaagggta tgtcaatctg ctgcccgttc
      901 agcataaacg gtctcgtgat ccgggcgaca gcgcggaaat gatgcaagca cgccgcgcat
      961 tcttagatgc cggacattat cagccgctgc gtgatgcaat tgtcgcccaa ctgcgggaac
     1021 ggcttgatga aaaggccacg gcggtgctgg atattggctg tggtgaaggg tattacacac
     1081 acgcatttgc cgatgcgttg cccgaaatca ccacgtttgg cctggatgtc tcgaaggtag
     1141 cgataaaagc ggcggcgaaa cgctatccgc aggtcactct ttgtgtcgct tccagccacc
     1201 gtttgccgtt ttccgatacc agtatggacg ccataatacg tatttacgcg ccgtgtaaag
     1261 cagaagaatt agcacgagta gtgaagcccg gcggctgggt cattactgcc acgccgggac
     1321 cgcgacattt gatggagctg aaggggctga tttacaatga agtacatctt catgcacctc
     1381 atgcagaaca actggaaggt tttacattac agcagagtgc ggagttgtgt tatccgatgc
     1441 gtcttcgcgg tgatgaagcc gtcgcattat tgcagatgac gccgtttgcc tggcgtgcga
     1501 agccagaagt ctggcaaaca ctggcagcaa aagaagtgtt cgactgccag acggacttta
     1561 atattcacct ctggcagcgt tcttattaac cgtggaagtg cgtccagagg atctggacgc
     1621 cgatgccgat cagcaccagc ccgccgagaa tttccgcttt tttcccaata attgagccga
     1681 taaagcgacc aaccatcatc cctaatgtcg acataatcaa ggttgcacaa ccaatggcca
     1741 atgcggtcgc gataatgttg acctgaagga aagcaagacc aacacccaca gccatggcat
     1801 ccaggctggt ggcaatcgcg gtggttacca gtagccagaa accgtgccga cggcgcggct
     1861 cttcatcttc atcatctgcg ccacgaaaac cctcaataat cattcgcccg ccgaggaata
     1921 tcagcagcac aaacgcaatc cagtgattcc attcaaggac aaaccggctg gctaacatgc
     1981 ccattcccca gccgatcagc ggcgtcaggg tttcgacggc accaaaaata aggccggttc
     2041 gcaatgcttc agaaaatttc ggtttatgga ggggggcacc tttaccgatt gatgcagcaa
     2101 atgcatccat cgacatacca aacgcaagaa gaacagtagc agtgatattc atgacaacgt
     2161 cctgaccggg gtatccataa aacacatcat cgcccccagc aaacatgcat acatgaacaa
     2221 caagccgcga tgggttttcg cgttcatgca ggcatagcga ttgatgtgca tatggtctcg
     2281 cctgactgaa agtcttcagt ccgtacgtgc cacgttttgc aacgagtatg ttgacacgta
     2341 catttccgga atctggaaat cggctactcc ccaaatgagg gcgcaacctt aacatatttt
     2401 gagaatataa aacaactgtt ggaatatatt atttcattgc tttattgata acgattttca
     2461 tttatgttta tat
//
LOCUS       DAZPAB010000331         2439 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZPAB010000331 DAZPAB010000000
VERSION     DAZPAB010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.334"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            129..638
                     /gene="eco"
                     /locus_tag="ACHUPG_004122"
     CDS             129..638
                     /gene="eco"
                     /locus_tag="ACHUPG_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR0863489.1"
                     /translation="MKKESKKMKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     TGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAEVR"
     gene            complement(760..2384)
                     /gene="mqo"
                     /locus_tag="ACHUPG_004123"
                     /pseudo
     CDS             complement(760..2384)
                     /gene="mqo"
                     /locus_tag="ACHUPG_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
BASE COUNT          587 a          658 c          597 g          597 t
ORIGIN      
        1 ctcatcagaa tttaacacta aaagagcagg taaaattgtc tgaatgttct ttaagttatt
       61 cataaagcaa attaataaat ctgatgaata tgttaacctt cagcgacatc atcggtgaaa
      121 acctataaat gaagaaggaa agcaaaaaaa tgaagaccat tctacctgaa gtattgtttg
      181 ccgctttcgc taccacttcc gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg
      241 cgccttatcc acaagctgaa acaggcatga agcgtcaggt gattcagtta accccgcaag
      301 aagatgaatc taccctgaaa gtagaactgt taatcggtca gacgctggaa gtcgattgca
      361 atttgcatcg tctcggcggg aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt
      421 attatgtctt tgataaagtt agctcgccgg tttccacgat gatggcctgc ccggatggca
      481 agaaagagaa gaaatttgtc accgcgtatc tgggcgatgc tggaatgctg cgttacaaca
      541 gcaagctgcc gatcgtggtg tatacgccag acaatgtaga tgtgaaatac cgcgtctgga
      601 aggcggaaga gaaaattgac aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc
      661 cgttggttgg ataaggcggt tacgccgcat ccaacatcta acgcccgagc cggttgcctg
      721 atgcgacgct ggcgcgtctt atcagtatac gccacatcat tacaacgcaa tatccgccac
      781 ttctttttga acgggttgcg gtttcaactg cggtttcggc gtactatctg ctgcttgcgg
      841 tttgtcgtat ttcagcccca gcacttcgct ggtgtactgc aactcgcgtt ctgttgccgc
      901 tacatcaccg ttcagcttgc gtccataaga cggaacgatc gctttcaacg tagcctgcca
      961 ttgcgggctg gaaacgcgat cgccaaatac tttttccagt agattcaaca taatcggcgc
     1021 ggcggttgac gcccctggcg atgcccccag cagcgccgca atggtccctt gctggtcact
     1081 gaccacttcg gtacccagac gcagtacgcc acctttctcg gcatcacgct tgataatctg
     1141 cacacgctgc cccgcttgcc acaaacgcca gtcctctttt ttcgcttgcg gatagtactc
     1201 tttcaacgct tcaaaacgat cctcttcact caacatcacc tgactcacca gatatttcac
     1261 cagatcgaaa ttatccagcc cgacgtgcat catcggcatc acgttagagg tggtggtgga
     1321 actcatcaga tcccacaatg aaccgttttt gaggaatttg gttgagaagg tggcaaatgg
     1381 cccaaacagc actacgcgtt taccgtccag aacacgggta tcgatatgcg gaaccgacat
     1441 cggtggtgcg ccaacggatg ctttaccgta aactttcgcc agatggcgat taaccacgtc
     1501 tggattttcc gaaacaagga actgtccgcc caccgggaaa ccggcgtagt ctttcgcttc
     1561 cggaatcccc gattcctgta acagcttcag cgccgcaccg cccgcgccga taaagacaaa
     1621 tttggcgcgg atgttctgtg cagtgccgtt tttcagatcg gcaacggtaa cggtccaggt
     1681 gttatcgtca ttacgcttta gggcgcggac ttcgctgctg agttgcagcg agaagttaga
     1741 tttcttctgt aaggaagcaa ttaactggcg ggtgatctca ccatagttca catcagtgcc
     1801 aatttctgtt gcggatcgcg cccttccatc actaacggtg cccactcttt gatctgcgcg
     1861 tgatcttcag agtaacgcat accgcgaaac agcgagcttt gttgcaacgc ggcgtaacgg
     1921 gcgcgcagga aattaacgtt atcctcgccc cagacaaagc tcatatgcgg aacggtattg
     1981 ataaatgaac gcggagtacg cagcacgccg cgctcgacct ggtgcgccca gaactggcgg
     2041 gaaatctgaa atgcttcgtt aatggcgact gctttttcaa tactgatgct gccatcggcg
     2101 ttttgcgggg tgtagttcag ttccatcagt gcagaatgcc cggttccggc gttgttctag
     2161 ccgttcgaac tctcctgcgc gcccccctcc aggcgctcca ccatggtcat cgaccattca
     2221 ggctccagct cgcgtcaata ggtccccaac gtggcgctca taatgccgcc gccaatcaac
     2281 agtacatcag tttcctgctc ctcggacgct ttcgcttttg ccgccatcga aacggcatta
     2341 agccccacgg ccatcgagaa gagcatggca gtcacttttt tcatcttgtt aatgccttac
     2401 ttttagtcgc tttattgcag gtgagatttg cgcggcatc
//
LOCUS       DAZPAB010000332         2437 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZPAB010000332 DAZPAB010000000
VERSION     DAZPAB010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.335"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..360
                     /locus_tag="ACHUPG_004124"
     CDS             <1..360
                     /locus_tag="ACHUPG_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR0863490.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="ACHUPG_004125"
     CDS             361..726
                     /locus_tag="ACHUPG_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0863491.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1411
                     /locus_tag="ACHUPG_004126"
                     /pseudo
     CDS             723..1411
                     /locus_tag="ACHUPG_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
     gene            1605..1680
                     /locus_tag="ACHUPG_004127"
     tRNA            1605..1680
                     /locus_tag="ACHUPG_004127"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1638..1640,aa:Ile,seq:cat)
     gene            1682..1758
                     /locus_tag="ACHUPG_004128"
     tRNA            1682..1758
                     /locus_tag="ACHUPG_004128"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1716..1718,aa:Lys,seq:ttt)
     gene            1761..1836
                     /locus_tag="ACHUPG_004129"
     tRNA            1761..1836
                     /locus_tag="ACHUPG_004129"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1794..1796,aa:Thr,seq:tgt)
     gene            1840..1914
                     /locus_tag="ACHUPG_004130"
     tRNA            1840..1914
                     /locus_tag="ACHUPG_004130"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1873..1875,aa:Gly,seq:tcc)
     gene            2207..2422
                     /locus_tag="ACHUPG_004131"
     CDS             2207..2422
                     /locus_tag="ACHUPG_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR0863492.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          640 a          521 c          653 g          623 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc gcccggccct ttagctcagt
     1621 ggtgagagcg agcgactcat aatcgccagg tcgctggttc aaatccagca agggccacca
     1681 accgccacta gctcatcagg aaagagcgtc aaccctttaa gttgagtgtg cgaggttcga
     1741 gtccccggtg gcggtccagt gccgacttgg ctcagtaggt agagcaactg acttgtaatc
     1801 agtaggtcac cagttcgatt ccggtagtcg gcaccatatg cgggcatcgt ataatggcta
     1861 ttacctcagc cttccaagct gatgatgcgg gttcgattcc cgctgcccgc tccagtcaga
     1921 gtctttcagt ctgcgatgat gggaaatccc ggagtgactg aaagacgttt aagttatgaa
     1981 tgatcgcttt tttttgcaaa attgctgtgc agaaatacta accttcgggc aggcgatcat
     2041 tcataagcac tctgctttta ttccgattaa ctgtgagtgg tttgttggat agagtgcttt
     2101 ccttattgta tatatcgttt cgcccgcttt tgcggttttt tcttttcaaa tccctttcat
     2161 ttctcagtgt aaaactacgc catccgttat ttgcggaggt gaggctatga aatccatgga
     2221 caaaatttca acgggcattg cctacggcac ctccgcaggc agtgctggct actggttttt
     2281 acagtggctt gatcaggtca gtccgtcaca gtgggctgcg attggtgtac tggggagtct
     2341 ggttctgggc ttcctgactt atctgacaaa tctgtacttc aaaatcagag aagacaagcg
     2401 taaggctgcg agaggtgaat aatgtcgcca tcattac
//
LOCUS       DAZPAB010000333         2412 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZPAB010000333 DAZPAB010000000
VERSION     DAZPAB010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.336"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..545)
                     /gene="ygeQ"
                     /locus_tag="ACHUPG_004132"
     CDS             complement(<1..545)
                     /gene="ygeQ"
                     /locus_tag="ACHUPG_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeQ"
                     /protein_id="HFR0863493.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDG"
     gene            complement(1207..2196)
                     /locus_tag="ACHUPG_004133"
     CDS             complement(1207..2196)
                     /locus_tag="ACHUPG_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863494.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(2280..>2412)
                     /locus_tag="ACHUPG_004134"
     CDS             complement(2280..>2412)
                     /locus_tag="ACHUPG_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863495.1"
                     /translation="REQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWMDSL"
BASE COUNT          645 a          553 c          577 g          637 t
ORIGIN      
        1 attccatcca gtcgttttgg gttgctaatg atgtcctgca tccgcttaat ctggtcgggc
       61 gtagacatat aaccccacag ggtgagcaga accttttttg cggctacact atttccttgg
      121 tctgatacta atgccatata gccccataaa ctatccctca gccagacagc atcatagtgg
      181 gtttctgccg ctgttttgtc atcttcgaaa gtagaagcaa gaataaacgt tccccagggt
      241 ttctccgtca acgttaactt tcgtgtgttt tctgttaatg ttagcaatcc ttgagtttcg
      301 tctttggtat agtgactctt tatttcattt tttaatttgg ctgagtgaat cagaagacga
      361 taattaggat acttatctgg atttaacgat aaataacttt tatctgttgc tgcccccctg
      421 gaattaagaa tgttaaatac agattcgctt gtcacttctg cctctgtcgc ctggcatgtg
      481 ccgcacgaaa atataccagc gagcagaaga gttaatgcag atttcccttt cataaaataa
      541 tccatcaatc gtttgttaaa agcgtagata accacttatt caacaacttg aaaataaggt
      601 attttacctg agttgttaca aaaggattgc attgcgtaaa cgctttttat ttacaaaaaa
      661 atggggaagt gttacagcga ttgcgaaaag cctgaagtga ttgaattaag ataatataga
      721 aaatcagata cagcaaaaat ggcagattga tcaggtgaag ataaattgga gcgggcgaag
      781 ggagcctacg acaaatcgaa gacaaccagc taacctattg tttcttaaag acaggtgaat
      841 ccagttggtt gtagtgatag tcacacattt agagatagaa ggtcaagact tgaagcacgt
      901 aatattccct ctaaaggaat agaactcacg gtctgactac ccggcccttt tggtctggct
      961 caggtcaggc tatgggggct tttggctcgc tctttatatt agatgaggtc tcacattttt
     1021 cagtcaaaac tcatcgtcta atagcccata gccagaccac cagtcccagc tttagcagga
     1081 agaccacgag atagactttg tgtaggtcca tttggccctc ctactttctg tattcacaaa
     1141 ctagattact agtacaaaat aaaggccaga catccagttc ggaagtctga ccaatagtcg
     1201 ttattgttag ttagcttctt tagtgaagct caggcgtttg actacaggca ggacagcagt
     1261 agccgagcca gtacccatct tgatggtcgt agcagcttca gctacaacaa acaccttgcc
     1321 gagaatctca aggtcgtgag actgggagtc tccatcacgt gaaaggacca cagtaatgct
     1381 ctcgccatcg tgacggaagc aaccgaagct cagagccgta aaacgcttag ccatcatgaa
     1441 cacgccacgg tcttcaccgt tgtaataggc gacgtcagtc cccagcatat cgacgatagc
     1501 gtcttcacca ccgaagccct gcttagcagc cagacgggag agagccttga ggtgtgactc
     1561 atgggcgata atgccgtagt cagaaacctc agaaccgaaa gaagactccg tgttctcgta
     1621 cagggcatcc atgatagcgt cagccttagc ggtcaggcta ccagtcaggg ccggaagttc
     1681 tacggtcttc agcagctttg aggactccag cacgtcaacc atcttagagt taatggtacg
     1741 aatcacgtta tcgcagatgt tcagtgcgtc ctgctggagc gtcccctgag tgaaaccagt
     1801 ggtcgacttc tggtcccaaa gctttgtttg gtgagacacg aggatcgcct gttcaatcgg
     1861 ctcaacggtg atttcagtgc cagacggagc ggtcacaact tcaggtcgct taccaccacg
     1921 gacagggacc agcacagagc ggtcagccag agtgtgagca atgattggag tcggtaagct
     1981 gttacgggtc ggcaggactt ctacagggaa cgggatgtta tcttgagcgt ctttaagtac
     2041 accgttcagc atagccttaa cagtcttagc taactggagg gcatagacct gagggtcggc
     2101 gttagtcggc agaccttcat tgacctgaat gttgtcggta tcggtgatgc gtactggctc
     2161 aatgccaaac tggaaagcgt ttttagtcat attcattata attaatctcc ttgagtcaaa
     2221 tacttgaagt ccctaacagg cagtcaggga ccgtgtgttc tcagatgagg gacgcttgct
     2281 tacaagctgt ccatccagtt accgcctgac tcaaggtcag cgacgatagc ggcacgtgag
     2341 cgaccagcgg aatgagtagc gctgcggagg ttttcaccat aggtccggcg ttcagctttg
     2401 acctgttcgc gg
//
LOCUS       DAZPAB010000334         2410 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZPAB010000334 DAZPAB010000000
VERSION     DAZPAB010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.337"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1..108
                     /locus_tag="ACHUPG_004135"
                     /pseudo
     CDS             1..108
                     /locus_tag="ACHUPG_004135"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap.; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
     gene            60..233
                     /locus_tag="ACHUPG_004136"
     CDS             60..233
                     /locus_tag="ACHUPG_004136"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFR0863496.1"
                     /translation="MPSSAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALR
                     MLQEYIRIQCLGG"
     gene            complement(322..834)
                     /locus_tag="ACHUPG_004137"
     CDS             complement(322..834)
                     /locus_tag="ACHUPG_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR0863497.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1029..1793
                     /locus_tag="ACHUPG_004138"
     CDS             1029..1793
                     /locus_tag="ACHUPG_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863498.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1744..>2410
                     /locus_tag="ACHUPG_004139"
     CDS             1744..>2410
                     /locus_tag="ACHUPG_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR0863499.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          679 a          538 c          611 g          582 t
ORIGIN      
        1 atgctcttcg gcgcaagctt gataatggtg gtcgggtgct cgtcaaagga aaatgccctg
       61 tgccatcctc agccgaaacc tccagcgcct ccggcatggg caatgatgcc accgtcgaac
      121 tctctccagt tgctggacga aacgttctcg gtatccggga cggaatcatc agcgaccaga
      181 cagcattgag aatgcttcag gagtacatca ggatccaatg ccttgggggg tagcggtaat
      241 tttactcatt atccttcaaa tcagattctg ttgtcagagg aatgggggaa gctggattcg
      301 caagagcttt ttatgggcag tttagctaaa aaaaggcaac acgaagctaa aactaccaac
      361 ataaagtgct gaccagctag cagctgaaat cagactcact gtatacactt tcactggatg
      421 aagttgcaac attcctgcca ccaaaggagc aatgtaacgc agcacggcga taaagcgcga
      481 agtgaaaagt attgacacag agttattttg caactgaagg cgaacccgtt ctattgtatt
      541 ggaacggctc gatattatac atgcaagttg agggatatgc ccaattatta taccaagatg
      601 gtaattaacg atagtaccgc accatgcgcc actcatgacc gtgattccag cttcccatgg
      661 tgaaagagtc gtcttgctga ctgtaattac agtggccatc ataattgatg ctggtggcaa
      721 tatcgccgaa atgagtaaag tggattttgt gaacgctatt ataaaaagaa gcccccatag
      781 gcgatatgga tgaagtacaa aatagttcat caaagcattt atccactcca tcaatgtctt
      841 tccttctgtg aatttttctt tgctatttaa ccgtgatacg acttaattta ggcttaaaca
      901 ccttacattt acaaggtact cctgtggggg gccctgccac ggagcgtcgc aggagtgagt
      961 gatgaggaag aattaaagca acctattgtg acaggcagcg ataacagtaa atcacaggaa
     1021 gcataatcat ggcaaaaccg gactgggagg ccatcgaatc ggcataccgg gccggagtcc
     1081 ttagcctccg tgatataggc gagaaatacg gcgttacaga aggggctatc aggaagaggg
     1141 ccaaaaagct tgattgggca cgcagtggcg gtacgcaggt ttgcaaaaat ggtacgcaaa
     1201 aaaggaaagt gcgtaccagc agaaagcctg ccattactgg ccttacacaa aaaagtacgc
     1261 aactaaaaac agaatctaca ccggatacga aaccgatacg cggaatgcgt accgatcccc
     1321 cgactaaccc attccaaccc ggtaaccagc aggcattaaa acacggtggt tatgcccgtc
     1381 gccttctgct caaagatgag gtgatagagg acgctaaagc gttgacgctc gaggacgaat
     1441 tatttcgcct tcgtgctaac aaccttgtcg ctgcagagaa tattggtcgg tggttggtgt
     1501 cgctggaaga tgctaatggg gaccaggaaa ggaagatgct gattgaaaat atcagcgccg
     1561 ccgagaaagc aatgatgcgc aatacagttc gtattgagtc catcgttggc acgcttgcga
     1621 cggtaggaaa aatatttgct gatacagcct accgcaaggc cgccactgat aaggtgtctc
     1681 tggaggctga tcgtcttcgc cgtgatgcag gtattgatga tggcaatgga gagcgtgacc
     1741 tcaatgactt ctactctgac atccaaaccg acgctgaatc cggctttacg tagtttctgg
     1801 actatgcggg cacgtaacaa agtgctttat ggtggtcggt catcgtcaaa atcatgggat
     1861 gccgctggca ttgccatatt tctgtcgaat aaatacaccc tgcgtttttg ttgtgcccgt
     1921 cagatccaga ataaaatcga agagtcggtg tataccctgc tcaaaattca gatagacagg
     1981 tttggtctgc ggcaccgttt ccgtattctg aacaacaaaa tcattaaccg ggttactggc
     2041 tcggaatttg ttttttatgg attatggcgc aacatcgaag aaattaagtc actggagggg
     2101 atcgatatgt tgtggttgga agaagcccac gcactgacgg aataccagtg gaaaattctg
     2161 gagccaacga tccgtaaaga gggttcggaa tgctggttca tattcaaccc cggacttgtt
     2221 actgatttcg tctggcgcaa cttcgttgtt gatccgcccg aaggcactct catccgcaaa
     2281 attaactatg acgaaaatcc gtttctgtct gacaccatgc ttaaggttat cgacgcggcg
     2341 cgacgccgtg atccggatgg ttttaaacat gtgtatgagg gcgttccgga gtctgatgat
     2401 gatgcggcaa
//
LOCUS       DAZPAB010000335         2403 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZPAB010000335 DAZPAB010000000
VERSION     DAZPAB010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.338"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(1..671)
                     /gene="chbR"
                     /locus_tag="ACHUPG_004140"
                     /pseudo
     CDS             complement(1..671)
                     /gene="chbR"
                     /locus_tag="ACHUPG_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="ACHUPG_004141"
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="ACHUPG_004141"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR0863500.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1088..2403)
                     /gene="chbC"
                     /locus_tag="ACHUPG_004142"
                     /pseudo
     CDS             complement(1088..2403)
                     /gene="chbC"
                     /locus_tag="ACHUPG_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
BASE COUNT          667 a          620 c          525 g          591 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactacg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtcc gctgatactc tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttcca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     1441 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagc
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     2161 ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     2221 tggttgaggc gtcgaggcga atacctaagg aataaaaaac gacccctccc caaagcttag
     2281 aaaaacgttg ttaattaata caaacatggc ccccgcaagg gttaacggca ttaagcgaat
     2341 aaagccattt ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag
     2401 gag
//
LOCUS       DAZPAB010000336         2402 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZPAB010000336 DAZPAB010000000
VERSION     DAZPAB010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.339"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="ACHUPG_004143"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="ACHUPG_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR0863501.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1861)
                     /gene="phoA"
                     /locus_tag="ACHUPG_004144"
     CDS             complement(446..1861)
                     /gene="phoA"
                     /locus_tag="ACHUPG_004144"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR0863502.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="ACHUPG_004145"
     CDS             complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="ACHUPG_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR0863503.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
BASE COUNT          548 a          615 c          556 g          683 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagcttgt tgcgttgctt gattattaca ttcacgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cagttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca agggttcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca tcggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt tttttattca gcgcatagtg agtgtattgc ccggtaagcg
     1561 gtaaggcatc gataccttta aaaaagccgc ccgcaccttc ggcataatta cgtgcggcag
     1621 taatttccga atcccccatc ccatcgccaa tcagcaaaat aatatttttt gcaggtttat
     1681 cgctaagaga atcacgcaga gcggcggtct gatcacccgt taagcggcga gcaccgccgg
     1741 gtgcagtaat atcgccctga gcagcccggt tttccagaac aggcatttct ggtgtccggg
     1801 cttttgtcac aggggtaaac agtaacggta agagtgccag tgcaatagtg ctttgtttca
     1861 ctttattttc tccatgtaca aatacattaa aaaataaaaa caaagcgact ataagtctcg
     1921 gccgtgacaa ctttatgaca gctgttgaaa agattaactt tttactgaca aggatgcttc
     1981 aataacttct ttacgtaatc gcgcagcagc tctgtatcgt cgtcaggaat gctggcatcg
     2041 ggctttacct cgtacagcgc cccctctacc tgatcaatca accgctgttg gtcattttgc
     2101 gccatattgc gaagcattgc agtgacgata atctccaaag cttctacctg cgcacacagt
     2161 tctttcgact cttcttcttt ttgggcaagc ttaaataaca actcagcaat gagatttttc
     2221 atgtctgtat ttccttatcc aaagtatgga gaagttaaca ttatgtttca caacttcata
     2281 gtgtctggaa gtattatttt tatgacaaag gaaatacttt gcgcaaatat tttttgtttg
     2341 attgccagcg aaacgtttct cttatttaaa taattaccca cacaaaatat aggcttttaa
     2401 tt
//
LOCUS       DAZPAB010000337         2383 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZPAB010000337 DAZPAB010000000
VERSION     DAZPAB010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.340"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            254..433
                     /locus_tag="ACHUPG_004146"
     CDS             254..433
                     /locus_tag="ACHUPG_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR0863504.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            519..1019
                     /locus_tag="ACHUPG_004147"
     CDS             519..1019
                     /locus_tag="ACHUPG_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFR0863505.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            1065..1571
                     /locus_tag="ACHUPG_004148"
     CDS             1065..1571
                     /locus_tag="ACHUPG_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFR0863506.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(1631..2269)
                     /gene="ompW"
                     /locus_tag="ACHUPG_004149"
     CDS             complement(1631..2269)
                     /gene="ompW"
                     /locus_tag="ACHUPG_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR0863507.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
BASE COUNT          761 a          523 c          495 g          604 t
ORIGIN      
        1 atttttccgc ccaacagtaa tataatcaaa caaattaatc ccgcaacata acaccagtaa
       61 aatcaataat tttctctaag tcacatattc ctcaggtaat tgttaatata tccagaatgt
      121 tcctcaaaat atatttccct ctatcttctc gttgcgctta atttgactaa ttctcattag
      181 tgactaattt taatgagtgt cgacacacaa cactcatatt aatgaaacaa tgcaacgcaa
      241 cgggagaaat aacatggccg aacatcgtgg tagttcagga aatttcgccg aagaccgtga
      301 gaaggcatcc gacgcaggcc gtaaaggcgg tcagcatagc ggcggtaatt ttaaaaatga
      361 tccgcaacgc gcatctgaag cgggtaaaaa aggcggtcaa caaagcggtg gtaataaatc
      421 aggcaaatcc tgattcaccg ttaattatta atatcagctg ataaagataa ttgcatctgc
      481 gggccgtatg gctcgcagtg atttcactgg agaaaaatat gaatatgaag accattgaag
      541 atgtatttat tcaactgctt tcagatacct acagcgcaga aaaacaatta acccgggcac
      601 tggcaaaact cgcaagagca acatcaaatg aaaaattaag tcaggctttt catgcgcacc
      661 tcgaggaaac tcatggacag attgaacgaa ttgatcaagt tgtggaatca gaatcaaatc
      721 tgaaaattaa gcgcatgaaa tgtgtggcaa tggaaggtct tattgaagaa gctaatgagg
      781 tcatcgaaag taccgagaaa aacgaagtgc gtgatgccgc actgattgcc gcagcacaga
      841 aagtcgagca ttatgagatt gccagttacg ggacattagc gacgctggct gaacaattag
      901 gttaccgtaa agcagcgaag cttctgaaag aaaccctgga agaagaaaag gccaccgaca
      961 tcaaactgac tgatctggcc cttaataacg taaataagaa agccgaaaat aaagcctgaa
     1021 atatgaattt taacttttag tcattttata aagaggacat tttcatgaat cgtattgaac
     1081 attatcatga ctggttacgt gacgcccacg caatggaaaa gcaagccgaa tctatgcttg
     1141 aatccatggc cagccgtata gataattatc ctgaactacg cgctcgtatt gaacaacatc
     1201 ttagtgaaac caaaaaccag attgttcaac tggaaactat tcttgatcgt aatgacattt
     1261 cacgttcagt cattaaagat tccatgagta aaatggctgc gcttgggcag tcaatcggtg
     1321 gtatattccc ttctgatgaa atagttaaag gctctattgg cggatatgtc ttcgagcaat
     1381 ttgaaatcac ctgttacacc tcactattag cagcagcaaa aaatgccggt gatacagtct
     1441 caattccgat catcgaagcg attttaaatg aggaaaagca aatggccgac tggctgattc
     1501 agcatattcc gcaaacaact gagaaatttt taattcgttc tgaaactgat ggcgtagaag
     1561 cgaagaaata atacccttta taccatgtcc ttattgaccc cgtatattac ggggtcgttt
     1621 ttgtgcggaa ttaaaaacga tatcctgctg agaacataaa cacccacgga tcgaggcgta
     1681 cgctatcgtg ttgctgtgca ccgcctagct tataattggc ggtggtatcg atatccatgt
     1741 accacactga catgttaacc aaccagtcac ggttaatcag ataatcaacc cccacctgcc
     1801 cggcagctcc ccaggaatct ttcagactga gatcggaaag ccccgcctct ttgccatgat
     1861 cgttaaatcc attatcaaag aaggtggtgt agttaatacc ggccccaacg taaggacgga
     1921 atttgctgct ggcatcacca aaataccact gcgccatcag tgttggcggc agatggtgaa
     1981 cggttgcaat atcgccggtc gcccgggtgc cgattttatg gcggaacggc gtcgctgcca
     2041 gtaattccac accaatgttg tcggtcgcca tataagtaaa cgtcaagccc agttgagtgt
     2101 tattggtcac gctgaatcca cccagacttc ctaacgtacc accagcacct tctgttggac
     2161 gtacggttgc agaacctgca cgcataaaaa attcgcctgc ttcatgcgca aaggcactgc
     2221 cagaaagaag agttgttact gccaaagccg ccactgttaa ctttttcata tccgctccgt
     2281 cgttatggtt ataaaaacgt gacgaatata cctacattgg agtaacaaat gatcctacat
     2341 agatcacatt acatatgata attcaccatt tattgatctg gat
//
LOCUS       DAZPAB010000338         2325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZPAB010000338 DAZPAB010000000
VERSION     DAZPAB010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.341"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            61..282
                     /gene="kil"
                     /locus_tag="ACHUPG_004150"
     CDS             61..282
                     /gene="kil"
                     /locus_tag="ACHUPG_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR0863508.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYILHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            282..452
                     /locus_tag="ACHUPG_004151"
     CDS             282..452
                     /locus_tag="ACHUPG_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFR0863509.1"
                     /translation="MTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYCSE
                     ICAEKDQMAHEL"
     gene            527..802
                     /locus_tag="ACHUPG_004152"
     CDS             527..802
                     /locus_tag="ACHUPG_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863510.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            902..>2325
                     /locus_tag="ACHUPG_004153"
     CDS             902..>2325
                     /locus_tag="ACHUPG_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFR0863511.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKTQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAE
                     LHEARKNIPPKNPVDADKLLAALR"
BASE COUNT          720 a          531 c          528 g          546 t
ORIGIN      
        1 taagtattaa ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc
       61 atgattgctc atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
      121 tatattcttc atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa
      181 ctttatattc gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc
      241 ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt aatgactaag aaaataaaat
      301 gtgcttatca cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc
      361 atttcatgcg tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg
      421 ccgaaaaaga ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat
      481 gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta
      541 tgaagccact gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg
      601 tattggctac gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga
      661 cggtccatca ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca
      721 cataggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat
      781 gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa
      841 gaacctggga catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac
      901 aatgaacacc gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag
      961 aaaacgtctc ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc
     1021 catctcccgt ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa
     1081 acctgttcgc gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac
     1141 ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc
     1201 atcttctccc tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga
     1261 agacatgaca gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact
     1381 ccaggcatta cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc
     1441 ccacaaaacc agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga
     1501 tactgacaga gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt
     1561 cgaagcatac atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa
     1681 cctcaccgat cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc
     1741 cactggcgta ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc
     1801 taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1861 attcatcacc atgcctggcg ggatggatta ttcccgcgcc atcgtggttg cgtcagtgaa
     1921 agaagcaccg attggtatcg aggtcatccc cgctcacgtc actgaatatc tgaacaaggt
     1981 actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg
     2041 ctcctctgcc ccgatgccgc aacgtgtaac agaagaagga aaacaggatg atgaagaaaa
     2101 aacgcagcca tctggcgcaa tggcagatga acaggcaacg gctgaaacaa tggaaccgga
     2161 tgcaactgaa catcatcaga acacgcagcc gctggatgct cagtcacagg taaattctgt
     2221 tgatgagaaa tatcaggaac tacgggcaga actccatgaa gcccggaaaa acattccgcc
     2281 caaaaatcct gtcgatgcag acaaattact ggctgccttg aggta
//
LOCUS       DAZPAB010000339         2303 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZPAB010000339 DAZPAB010000000
VERSION     DAZPAB010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.342"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(586..2190)
                     /locus_tag="ACHUPG_004154"
     CDS             complement(586..2190)
                     /locus_tag="ACHUPG_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0863512.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          596 a          555 c          507 g          645 t
ORIGIN      
        1 ttaatcttta attgtgtctt taagttaact ttaagttaag cccctgtagg gactagagac
       61 cataattaag atgattgtgt cttattgcta ttcacttatc gactgaccca gagcacacag
      121 gaagcattca ctttaagtag aacgcttggt gtctgtaatt ggtcatcccg cccctgaaag
      181 gatgcgataa gttacggtta gttgtcctag aagagaaaac tttttgtgtc ccttctccct
      241 atagtgagtc gtattaatta tccttgacaa gtagagaaaa ccatgagtca aaaacatgac
      301 cacacaggtc tgtagtaagt gtcatcctag aagagaaaac tttttgtgtc ccttctccct
      361 atagtgagtc gtattaatta ccccttgaca agcagagaaa actatgagtc aaaaacatga
      421 ccgcacaggt ctgtagtaag tgtcatccta gaagagaaaa ctttttgtgt cccttctccc
      481 tatagtgagt cgtattaatt atcccttgac aagtagagaa aactatgagt caaaaacatg
      541 accacacagg tctgtagtaa gtgtcatcct agactatggt ctcctctagg tctccttata
      601 aacatcagcc atcacattga ccagcctatc aacctcgacc tctttagcat aacggtcgta
      661 cgtaatagac cctgaactat gccccatgat cgcctgtgca tacgccaact cgaccccatg
      721 ttgttttaac tgggccgcaa ctgtatgtct aaacgagtga aacgctaagc cgctacggtc
      781 tacgaggact ttaggcaaca acgtccgatt aaaccattta ctcacctgct caccgtaacc
      841 gttcttcatg agacgcagac catcgaacac catagcgtca tcaccgtcag cgcctctacg
      901 ggtctctaca agcgccataa agtcagccaa tataaaacca taggccccat cgaccagagg
      961 cacacagcgg tcactatgag cgttcttgat gctcttaccg ggtagactac tatcgtcttc
     1021 attgatatga atataaacgg ttcccgcctc agtggtcctg acgtctttga cctgtagttg
     1081 ggctatttca ttgagtctcg ccccagtgat agcagccaag gcggtaacat aataatgata
     1141 cggcttacca gaggtcttct gagagtatgc cttagctgcg accagtagtt gccccacctg
     1201 ctccgtagag aaggcgttac gggcccctga ggctttacgc tgagggactt taagctccag
     1261 accctcagtc atgttctttt taatcaagtc attgcgcaca gcccacctaa agacagcagc
     1321 catcttaata agatacttgt tgttgatggt aacgacatcc agacaatctg tcttatcttc
     1381 acggctcaac aggtcagcaa gtgggacatc cttgaagcgc tgcttacggt tcctaggtaa
     1441 ctgctggagc acatcacgga ccctaagcat gtcagccctg ttagcgtctg cattgagacc
     1501 cagatggtcg aatatttcga ttaacgcagc gtgtgacgcc ttgttttccc ttaaggttgc
     1561 aggcttccag ttctgatagt tctccgcttc gtattgttca aagaggctcg ctatggtaat
     1621 gctaggcgta acttctggtt ttacttctgg agcattaaca gatagggaca cagagtctgc
     1681 ttggttatct tgcctatcaa tatcattaaa aagatcgacg taatctatca gaggctgact
     1741 gtctcccgct tcgatacgcc tcatacaggc ttttagcacc ttcagggcct cattgatgta
     1801 acgatgctgg tcaatgctta gcggctcact agccagagca tcggtaaggc tctcaccaag
     1861 ttctccgtat tggtcccggt agatatccct catgtcaggc tcaaagagat ctgaacggcc
     1921 catagacagt tcacactcag cgatgtcctt aaggtgttcc ctcatttcct cataggtagc
     1981 gttgggtctg tctgcgtgta ttgccttgag tgctgccttt atgtgccttg agtaagccat
     2041 agcggtgcgt ctatccttgg tccgtaatga tattgaggtc attctatcat tgtttgattg
     2101 tctgacccta agacggaagt agtagacgcc gttacgctgg tagagatagt agactgctgg
     2161 aagtttgtga ctactggata gctgaatcat atgtctgcat gccctgagag ggttacacag
     2221 tgggttacag taagtaactc tgcaaggagg cttaaagccg tatcccttag gattcagtag
     2281 gttggagtac tttaagaaag gtc
//
LOCUS       DAZPAB010000340         2250 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZPAB010000340 DAZPAB010000000
VERSION     DAZPAB010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.343"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..242)
                     /locus_tag="ACHUPG_004155"
     CDS             complement(<1..242)
                     /locus_tag="ACHUPG_004155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863513.1"
                     /translation="MFFSLSVLNLEDHLGLVISPQSPLSAGYITLRACRRSLPLRFST
                     VTPGGIVGFLIVSYRFNIPDRLAVHLSIHARPELRA"
     gene            671..2179
                     /gene="ltrA"
                     /locus_tag="ACHUPG_004156"
     CDS             671..2179
                     /gene="ltrA"
                     /locus_tag="ACHUPG_004156"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR0863514.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          574 a          553 c          666 g          457 t
ORIGIN      
        1 tgtgcccgga gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata
       61 ttgaaccggt aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac
      121 ctgagcggca gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat
      181 tgaggcgaga tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac
      241 atgaacccgt taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac
      301 aggccgtgca ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac
      361 atgtatgcga aacaccgggg cagcagcgtc tatcacactt gcgttgctga cttctgccaa
      421 cttgcggcaa gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag
      481 gcaggtaaac cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc
      541 tggctggcgg agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga
      601 agcagtttga atgtgtttgc gacgtgaatt aactgaccta acgaggtgaa gacctttgat
      661 aatcagcgaa atgcaacgca agcttgccac atgggcagcc accgatccgt ccctacggat
      721 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
      781 gctttcatca aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      841 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      901 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      961 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
     1021 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
     1081 cgcgatccgc acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt
     1141 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
     1201 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
     1261 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
     1321 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
     1381 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
     1441 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
     1501 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
     1561 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
     1621 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
     1681 tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc
     1741 cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga
     1801 acaactcaac cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc
     1861 caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg
     1921 caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg
     1981 tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat
     2041 actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa
     2101 tccctatctg aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat
     2161 ggcgttcgcc agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg
     2221 ggaggagagg cagggaaata gtccgactac
//
LOCUS       DAZPAB010000341         2244 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZPAB010000341 DAZPAB010000000
VERSION     DAZPAB010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.344"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..414
                     /gene="torD"
                     /locus_tag="ACHUPG_004157"
     CDS             <1..414
                     /gene="torD"
                     /locus_tag="ACHUPG_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR0863515.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(507..812)
                     /gene="cbpM"
                     /locus_tag="ACHUPG_004158"
     CDS             complement(507..812)
                     /gene="cbpM"
                     /locus_tag="ACHUPG_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR0863516.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="ACHUPG_004159"
     CDS             complement(812..1732)
                     /gene="cbpA"
                     /locus_tag="ACHUPG_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR0863517.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1993..>2244
                     /locus_tag="ACHUPG_004160"
     CDS             1993..>2244
                     /locus_tag="ACHUPG_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863518.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSL"
BASE COUNT          576 a          541 c          554 g          573 t
ORIGIN      
        1 acgctgacag tacgtgacga tgcccgtctg gaactggccg ctgacttttg cggcctgttt
       61 ctgatgaccg acaaacaagc ggcgctgccg tatgcatcgg cctacaaaca ggacgagcaa
      121 gagattaaac gcttgttagt tgaggcaggg atggaaacca gtggcaattt caacgaaccg
      181 gcagatcatc tggcgatcta tctcgagttg ttgagttatc tgcatttttc gctgggagag
      241 gggaccgttc ctgcgcgaag aatcgacagt ttgcggcaaa aaacactgac ggcgctgcgg
      301 caatggttac cagagtttgc tgcgcgttgt cgacagtatg acagctttgg tttttacgcg
      361 gcactaagcc agttattgct ggtgttagtg gagtgcgacc accaaaacag ataacgtcgt
      421 ttatgcgcct gaaaagacgc gtttagcgtc gcatcaggca ttatggcgca gttgccggat
      481 gcgtcatcac atccggcaaa cacacatcac ggatgagcta caaaccggga aagccgctgg
      541 cgtagcaggc ggttttcctg cttcaggtgc gcaatatcat ccattaacgt cagcgccacc
      601 gcgatccccg gccagtccag agccagttca tgacgcaggc gtaccgcgcg ttgcaccaca
      661 atggcggcat ggtcgtcaaa tacccaggtt gtttcctgga tctcacgcgg ttcaaccacc
      721 cccaaaccga caatttcatt caactcctct tcagagatgc cggtatgcag gcaaaattcg
      781 gtaatagtaa aagtcaccgt aacattagcc attatgcttt cccccaatct ttacgtggat
      841 caaaagacga ctgggcgtct gccagttgct gccacagcgc ggcagtgttt tcatccggtt
      901 tcggcggcat cacgattttc agtaccgcat acagatcgcc ggtctgtttt ttgctcacca
      961 gacctttgcc tttaacgcgc aatcgttgcc cggcctggct gcctggcggg atagtcagca
     1021 aaatgctttc tttcagtgtt ggaacggtga ctttagtacc cagcgccgct tcccacgggc
     1081 taaccggcac cacaatttcc agatcctggc cgacaatatc gaacagcgga tgtggcgcaa
     1141 tatgaatcac cagccacaaa tcgccatttg gaccgccgtt ttcgcccggc gtcccctggc
     1201 ctttcaggcg gatgcgttga ccattgccga cgcctgccgg gatcttcaca ttcagcgttt
     1261 tcgggatttc ctgttcgatc atgccaaagg cgttataaac cggcaggtta tagctgatgg
     1321 tacgcttatg ctcagtaagc gtttcttcga ggaataccgc cacttcgatt tcaatatcgt
     1381 ggccacgtgc ggcggggcgt tgacggctct ggcgggcatg ctgaccgaaa attgacgaga
     1441 agatatcgtc aaaatcttcg gcattaaaac tctgaccgtc gccatgatgg aactgacggt
     1501 taaattgcgg atcgttgcga tgttgccaca tctgatcata ctcagcgcga cgttgttcat
     1561 cacttaacac ttcccaggct tcagcgacct ctttgaagcg ggcttcggca tccggttctt
     1621 tgctgacatc aggatggtat ttacgggcca gtcgacgata ggcggtcttg attgtcttga
     1681 gatcgtccgt cggtttcacg cccatgatgg cgtaataatc ctttaattcc atagcgttat
     1741 ctcgcgtaaa tcaacacaaa ttgaaggaac ccctgtaagg taactcctat aagtgtaggg
     1801 taatcctcaa aatttcatat gccaacacag aatatgttat tgaaatcatc gcggagagga
     1861 ggtcgccatc aagatgggtt gctgaacata ttttaaacag gtgaaaaagg gtgagcgatt
     1921 tttgatagtt gaaccaggca ctttaagttt aactagggcg tcattattta ttaaatttta
     1981 tagacgctat atatgggtag taatatacat ggaattagtt gcactgcaaa taattatttg
     2041 aaacaggcct ggaacgatat aaaaaatgag tacgaaaaaa atcaaacata ttcaatcacg
     2101 ctttttgaaa acacactggt gtgttttatg cggttgtaca atgaactcag acgtaaagta
     2161 aatgaagagg atactccatg tctggaatgt gaatcactag aaaaagaatt tgaggaaatg
     2221 cagaatgata atgatctatc atta
//
LOCUS       DAZPAB010000342         2230 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZPAB010000342 DAZPAB010000000
VERSION     DAZPAB010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.345"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..373
                     /gene="tnpA"
                     /locus_tag="ACHUPG_004161"
     CDS             <1..373
                     /gene="tnpA"
                     /locus_tag="ACHUPG_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFR0863519.1"
                     /translation="RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQY
                     RKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRL
                     KGKLTPALLQILIREIKGSSH"
     gene            373..720
                     /gene="tnpB"
                     /locus_tag="ACHUPG_004162"
     CDS             373..720
                     /gene="tnpB"
                     /locus_tag="ACHUPG_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0863520.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            740..1969
                     /locus_tag="ACHUPG_004163"
                     /pseudo
     CDS             740..1969
                     /locus_tag="ACHUPG_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1973..2132
                     /locus_tag="ACHUPG_004164"
                     /pseudo
     CDS             1973..2132
                     /locus_tag="ACHUPG_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          542 a          594 c          620 g          474 t
ORIGIN      
        1 gcggcctaat tttccttatg aatttaaaat cgctttagtg gagcagtcac tgcagcccgg
       61 agcctgtgtg gcgcagatcg cccgtgaaaa cggaatcaac gataacctgc tcttcaactg
      121 gcgccatcaa taccggaaag gtggcctgct gccttccgga aaaaatatgc ctgcactgct
      181 tcccgtgacg ttaacgccgg agccggataa taaaatcccg gctccggcac aggaaccaga
      241 gcagataaat acaccgtccg acagtctgtg ctgtgagctg gttctgccgg ccggaactct
      301 caggcttaaa ggtaaactga cgccggcgtt attacagata cttatccgcg aaataaaagg
      361 gagcagccac tgatgatatc tttccctgca ggttcgcgta tctggctggt tgcaggtatc
      421 accgatatgc gaaatggctt taacggcctg gtctcaaaag ttcagaacgt cctgaaggat
      481 gacccgttct ccgggcatct gttcatcttc cgcggacgcc ggggtgacca gataaaagtg
      541 ttgtgggctg acagtgacgg actgtgcctc tttaccagac gcctggagcg gggccgcttc
      601 gtctggccgg tcacccgtga cggcaaggtg caccttactc cggcccagtt atccatgctt
      661 cttgagggca tcgactggaa acacccgaaa cgaacggaac gcgctggcat ccgcatataa
      721 cccgttgtaa agtgaggata tggacacctc acttgctcat gagaatgccc gcctgcgggc
      781 actgttgcag acgcaacagg acaccatccg ccagatggcg aaatacaacc gcctgctctc
      841 acagcgggtg gcggcttatg cttccgaaat caaccggctg aaggcgctgg ttgcgaaact
      901 gcagcgcatg cagttcggta aaagctcaga aaaacttcgc gcaaaaaccg aacggcagat
      961 actggaagca caggagcgaa tcagcgcact tcaggaagaa atggcggaaa cgctgggtga
     1021 gcaatatgac ccggtactgc catctcccct gcgccagtct tcagcccgta aaccgttacc
     1081 agcctcactt ccccgtgaaa cccgggtcat ccgtccggaa gaggaatgct gcccggcctg
     1141 tggtggcgaa ctcagttctc tgggatgtga tgtgtcagag cagctggagc ttatcagcag
     1201 cgcctttaag gttatcgaaa cacaacgtcc gaaactggcc tgttgccggt gtgaccatat
     1261 cgtgcaggca ccggtgcctt caaaacccat tgcacgcagt tatgccggag cggggcttct
     1321 ggcccatgtt gttgctggaa aatatgcaga ctatctgccg ttataccgcc agtcagaaat
     1381 ataccgtcgt cagggcgtgg agctgagccg tgccacactg gggcgctgga caggtgctgt
     1441 tgctgaactg ctggaaccgc tgtatgacat cctgcgccag tatgtgctga tgcccggtaa
     1501 agtccatgcc gatgatatcc ccgttccggt ccaggagccg ggcagcggta aaacaaggac
     1561 tgcccggctg tgggtctacg ttcgtgatga ccgtaacgcc ggttcggaaa tgcctccggc
     1621 ggtctggttc gcgtactcac cggaccggaa aggtatccat ccacagaatc atctggccgg
     1681 ttacagcgga gtgcttcagg ccgatgctta cggtggttac cgggcgttgt acgaatccgg
     1741 cagaataacg gaagccgcat gtatggctca tgtccggaga aaaatccacg atgtgcatgc
     1801 aagagtgcca accgacatca ccacggaagc cctgcagcgt atcggtgaac tgtatgccat
     1861 cgaggcagaa gtccggggat gttcagcaga acagcgtctg gcggcaagaa aagccagagc
     1921 cgcgccactg atgcagtcac tgtatgactg gatacagcaa cagatgaaaa ttcattcgct
     1981 gaaaatggaa tgtctccacg gagaacacta ttatccatcg ggaaatagtg cgggcaacag
     2041 tgtttagtta tatcgagtgc gattacaatc gctggcgacg gcaccgttgg tgtggtggtc
     2101 tcagtccgga acaatttgaa aaccagagcc tcacttacga ctgtgtccat attatgtggg
     2161 taggatcaat aaagacgttt tatatgtgtc tgcttgcagc cagaaaattt ataccctgca
     2221 ggtatcataa
//
LOCUS       DAZPAB010000343         2228 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZPAB010000343 DAZPAB010000000
VERSION     DAZPAB010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.346"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1..956
                     /gene="espL1"
                     /locus_tag="ACHUPG_004165"
                     /pseudo
     CDS             1..956
                     /gene="espL1"
                     /locus_tag="ACHUPG_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1128..1235
                     /gene="yniD"
                     /locus_tag="ACHUPG_004166"
     CDS             1128..1235
                     /gene="yniD"
                     /locus_tag="ACHUPG_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR0863521.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1288..2046)
                     /locus_tag="ACHUPG_004167"
     CDS             complement(1288..2046)
                     /locus_tag="ACHUPG_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR0863522.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          672 a          487 c          462 g          607 t
ORIGIN      
        1 tgctcagacc tgggctgata ggtatgttcg caactgtgca attgctattc tgcccctatt
       61 acctgtggga gttctagggg ctattttcgc gggtattgca tcacgaatgc catttgcccc
      121 tatacatcca tcggcaatgt tgttaataat ggctacaggc cagactcaac agcttattac
      181 attattcaaa cagttgccca tactccctga aaaagaaatc attgaaataa taactgcgca
      241 gaatagcgtt ggtacacctg ctttattttt ggctatgatg aacggacata ctgacaacgt
      301 gaaaatattt atgcaagaaa ttcagtcact ggtagataat cacatcattc atgaagataa
      361 tctggttaaa ttactgcaaa ctaaaagtgc taacgaaaca cctggacttt atatctccat
      421 gttgtatgga ttcgatgaaa taatcgatat ctttctgaat acattagcca ctccaatagc
      481 actaagagct tttaaacaaa aaactggtga tgagtatttt agccatgaaa atacatgatg
      541 gtgagccagg attatacgcc gcaatggaaa ataatcaccc tttgtgtgtc acacggttcc
      601 tctctaaaat taatggcatc gcctttaaat acaagttgag caaagctaac atcatggatt
      661 tattaaaagg agctacagca cagggaaccc ctgcattata catcgccatg agcaagggta
      721 atgaagacgt cgtgttatct tatatatcga cgctgggtgc ttttgcaaaa aaacattctt
      781 ttagtcaaca tcagttattt acactattgg ccgctaaaaa tcatgacaac atgtcagctg
      841 ttcatatagc cattcatcat aagcattata aaactgtaga aacatattat gctgctataa
      901 atgtcatcag ccaaagcctg agttttagtg ctgatgaaat aaagacgtat ttataaccag
      961 ctatttacga tatatatcga tacataattt tatttttttc ataaaacgca gaaacagtga
     1021 ccatgtgcac aacgccaccc gacggctgta ataacaaaaa atcccttgat gcctgtccct
     1081 tttgttacac tccgttatca cgcacaagag atatgcagga cactggtatg ccgactaaac
     1141 gctttgataa aaaacactgg aagatggtgg tggtgctact ggcaatctgt ggcgctatgt
     1201 tgttgctacg ttgggcagca atgatttggg gctgagcagt gacaaacgaa gacagccaga
     1261 ccgcataaca cggtccggca gataatttta catgctgtag cccagcgata aggttgtccg
     1321 acggtcggta tgttccggtg cagactctgg aggttcagag ttccatgtga cgttgtaagc
     1381 caccttcagt ccaaaatgtt cattaatagc aacatttaat gcgctttcgg agttcagcgt
     1441 tgtgtcttcc gcgccaaaga ccgaaacacc ctgcgtaaat ttagcgttgt cagtcaactg
     1501 ccatgcataa gccccggagg catagcccag cggctgggtt tcactggcat tgtcggtata
     1561 tttgtcgtaa cgcacacctg gaccgaattc aaagcggaaa ctgtgtaccg ggccattcaa
     1621 aaactgacga ccataacccg cggtcaacac atcgcgctct cgatagccgt tataacggtc
     1681 tgtcagccag cttgcctgac caaataaata gtcataatca gttaaattaa agcggctacg
     1741 cccgcccgcc gcatattttt ctgaagaacg ctcatcatta gaagaagtat tactggcgtt
     1801 cccccacagc gaccaggcag tggtttgtcc ataccaggtc atggtggtgt cagccgtaag
     1861 tgaggagctt ttcgtattgc ctgattgtgc aagatatcct gcgttcagat taccttcgaa
     1921 aggtttttta gcgctggcag gatcgtccat gacagtaaaa acggaatcat cggcagctgc
     1981 attcagtgac gcaaacatgc cccccgccaa cataacgatg gcaggaactg tcttcaaaag
     2041 cttcatttat caagagtccg tacaacaaaa aaagagacca tcgcggtccc ggaaactttc
     2101 ttaaggatca aagattagcg tccctggaaa ggtaacgaat tataaaaagg cgcgaataac
     2161 ttagcaatgt attcttattt cattttttga ataagcatgt ggcgaaaaca gatttttatt
     2221 tatatata
//
LOCUS       DAZPAB010000344         2218 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZPAB010000344 DAZPAB010000000
VERSION     DAZPAB010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.347"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="ACHUPG_004168"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="ACHUPG_004168"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR0863523.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="ACHUPG_004169"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="ACHUPG_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR0863524.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..2216)
                     /gene="arsR"
                     /locus_tag="ACHUPG_004170"
     CDS             complement(1881..2216)
                     /gene="arsR"
                     /locus_tag="ACHUPG_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR0863525.1"
                     /translation="MQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISRH
                     LALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQNC
                     SGDSKNICS"
BASE COUNT          589 a          611 c          555 g          463 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgg
//
LOCUS       DAZPAB010000345         2212 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZPAB010000345 DAZPAB010000000
VERSION     DAZPAB010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.348"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(1..213)
                     /locus_tag="ACHUPG_004171"
                     /pseudo
     CDS             complement(1..213)
                     /locus_tag="ACHUPG_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            488..763
                     /locus_tag="ACHUPG_004172"
     CDS             488..763
                     /locus_tag="ACHUPG_004172"
                     /inference="COORDINATES: protein motif:HMM:NF018642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HFR0863526.1"
                     /translation="MRDVQWFCPLTPVHTGTEIKPVEMPVTTITPVSDAGGLQDFIYW
                     RPDADRAGMEPVYVILNDPLDSGRFTRKQLNKNILNMQVILVFSIQE"
     gene            961..>2212
                     /locus_tag="ACHUPG_004173"
     CDS             961..>2212
                     /locus_tag="ACHUPG_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0863527.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLV"
BASE COUNT          567 a          458 c          513 g          674 t
ORIGIN      
        1 tgaacctcga tcggtgtggt ggattagccc ggcaggtggg cgctggctcc tgagcgccat
       61 aaacagggct ttacccgtca actctttcgt catgcgctct cccatggcgt aaccgacaat
      121 ttcgcacgta taaacatctt tgacgccagc gagatacagc catccttccc gggtggcaac
      181 atacgttata tctgcgaccc agacctgatt aggggggttg gggagcaatg gaacagtaaa
      241 cgccgttaag aagtcaattt aaatagccaa aaattaacta gccaatttac attgcaggtt
      301 gtaattttta accacctttt ttacgatagc caaattacct atacccatcc cggccctgcc
      361 aagccatacc cgctatcggc gatgaaaatg cttttgtgcg cggtcggagt ggtcccgacg
      421 agggttttac ccgaagtcgg ggcgtatctc cgcgttagcg ggccgtgagg gccgcttacg
      481 agcgtgtatg agagacgtac agtggttttg tccgctcaca ccagtacata caggtacgga
      541 aataaaacca gtcgaaatgc cggtaacgac gattactcct gtctctgatg ccggtggact
      601 acaagatttt atttactggc gtcctgatgc ggacagggct ggtatggaac ctgtttatgt
      661 gatacttaat gatcctctgg attccgggcg atttacgcgt aaacaactaa ataaaaatat
      721 cttaaacatg caagtaattt tggtattctc gatacaagaa taaatagtag taactactat
      781 agctgcaaat aatgttaaaa acatttctat gatcatcttt acatttttca tgattgacaa
      841 ttgtttttgg ttaaaaaaag acaactaatg agtgtgataa tgcaatactt tgagtgatta
      901 tataatgata agtaaacagg tttgcgacaa ttggttatca gtattacaga aggattaaat
      961 atgtcattat tcacagaacc taaggaaatt gaaagattac cctctgaaga gattgaacga
     1021 ttatatcccg tcctgcgata cagggtcttt atcagcattt tcctcggata tatggggtat
     1081 tattttgtcc gaaacaccac cagtgtgtta tccggtgtgc tgcacatgtc tgcaaccgag
     1141 atcggcatta tctcctgtgc cggtttttta tcctatggta tatcaaagtt tgtttccgga
     1201 cttatttctg acagaagtaa ttcaaaagta tttttgtctc ttggtttatt cctgtccgga
     1261 ctggtaaatt ttctgatagg atatattccc gggatcatca catccgttac gttattttca
     1321 acaatgtatt tacttaacgg ctggatccaa ggtatgggat atcctcctgg tgccaaaaca
     1381 ctggtgttct ggtatgaaca tcgggaaagg atcacctggg ccacactgtg gaatttatcg
     1441 cacaatgtcg ggggagctct ggcccccgtt ctgattggtt tcagttttgg ttttttcggc
     1501 gacagtgctc tggatcatgc ccgggccgca tttatcttcc caggagtact gtgtatggca
     1561 atgagtgtgc tgatttactt tattcaggtg gacagacctg tatcagtggg gcttcctccc
     1621 attgaagaat ggaaaggtaa cgtcgtttca cacccggcta aaggccgtga acaaggtccg
     1681 agactctcca ttcctgatat catcagaaaa catatcatcc gaaataataa actcatttac
     1741 tgctgtatct atggttcgtt tgtttatatt ctgagatacg gtattgtttc ctgggctcct
     1801 aagtttttaa gtgacagtct ggatgtcggt ggtaaggata tgggaaaact ggcatcaatg
     1861 ggggggggtt ctgtttttga aatcggtggt gtggctggta tgcttcttgc cggatacctg
     1921 tctgtcagac tgttcagaaa ctcaaaacca ttaacaaata ctttatttct ggcacttacc
     1981 attattttat taatcgcgta ctggtacgtg ccatcaggca atgaatatct ctggctaaac
     2041 tatacaatcc ttattctgtt ggggctggca gtgtatggtc cggtgatgtt tattggtctt
     2101 tattcaatgg agcttgttcc taaagaagct gccggagccg catcagggct gtcaggcaca
     2161 ttttcctata tcttcggctc aattgttgcg acactgggaa tgggacttgt tg
//
LOCUS       DAZPAB010000346         2190 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZPAB010000346 DAZPAB010000000
VERSION     DAZPAB010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.349"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            38..541
                     /locus_tag="ACHUPG_004174"
     CDS             38..541
                     /locus_tag="ACHUPG_004174"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0863528.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(587..1003)
                     /gene="rraB"
                     /locus_tag="ACHUPG_004175"
     CDS             complement(587..1003)
                     /gene="rraB"
                     /locus_tag="ACHUPG_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR0863529.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1165..2178
                     /gene="argF"
                     /locus_tag="ACHUPG_004176"
     CDS             1165..2178
                     /gene="argF"
                     /locus_tag="ACHUPG_004176"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR0863530.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT          564 a          525 c          541 g          560 t
ORIGIN      
        1 caaatgtata attactttat ttttattggg ttaaactatg aataacattg cgccgcaatc
       61 acctgtaatg cgtcgcctga cgctgcagga taatcctgct atcgccagcg tcatccgtca
      121 ggtttacgca gagtatggtc ttaccgccga taaaggctac accgttgccg atccgaatct
      181 cgacgaactg tatcaagtgt acagccagcc tggccatgct tattgggtgg ttgaatacaa
      241 tggcgaagtg gtcggcggcg gcgggattgc gccattaaca ggtagtgaag cggacatttg
      301 cgaactgcag aaaatgtact ttcttcccgc tattcgcggt aaagggctag cgaaaaaact
      361 ggccttaatg gcgatggagc aggcacgaga gatgggcttc aaacgctgct atctggaaac
      421 gaccgctttt ttaaaggaag ccattgcgct ttatgagcat ttgggctttg agcatatcga
      481 ctatgcgctt ggctgcacgg gccatgtcga ttgtgaagtg cggatgctac gcgaactcta
      541 aaaaaatgcc cgctagtatg tgctatcggg cattttaatc aattaattag tggcgaaccc
      601 cgtcatcgtc ttcatcgaca aaatcttcat cgtcgccatc ttcgccgtta ggatcttcaa
      661 agtaagtgcc ccaaccgtcg tactcaacgt caaatttctc cgccagcgtc atcagttgtt
      721 caacctgggc atcgatcaga tcggcattca acgcgcactc gctgaggatg tcgcagcaaa
      781 tcacgatatc accatcttca acttccagct cttctggatc ggtcacttcg taaccgagtt
      841 taaacgcttc aactgccgct ttttccagag tttccagatc gtctgcggaa agatggtgtt
      901 cgatggtgta cagcgcgtcc ggatcgctgc catcttccag taattcttca ataatcaaac
      961 gtgtttcttc acgctgttct tccagttgtt ccgggtttgc catggctcat tcctcttaat
     1021 gtcctgccga tactcttatt gtcacacacc actgacattg cctccacctt tgtgacaaag
     1081 atttatgctt tagacttgca aatgaataat aatacacata aattgagttt taattcattg
     1141 aggcgttagc cacaggaggg atctatgtcc gggttttatc ataagcattt cctgaaatta
     1201 ctcgatttca cgccagctga actcaacagc ctgctgcagt tagccgcgaa gctgaaagct
     1261 gataagaaaa gcggtaaaga agaagccaaa ctcactggta aaaacatcgc gctcatcttc
     1321 gaaaaagact cgactcgtac ccgatgctct ttcgaagttg ccgcatatga ccagggggct
     1381 cgcgttactt atcttggccc aagcggtagt cagattggtc ataaagagtc gattaaagac
     1441 actgcccgcg tgcttggtcg catgtatgac ggtattcagt atcgcggcta tggtcaggag
     1501 attgtcgaaa cactggcgga atacgctggc gtgccggtat ggaacggcct gaccaatgag
     1561 tttcacccca cgcagttgct ggcggatctc ctcaccatgc aggagcattt acccggtaaa
     1621 gcattcaacg aaatgacgct ggtctatgca ggtgacgcac gtaacaacat gggcaattcg
     1681 atgctcgaag ctgcggcgct taccggtctg gatttgcgtc tggtcgcgcc acaggcatgc
     1741 tggccagaag ctgcgctggt tacggaatgc cgcgccctgg cacaacaaaa tggcgggaat
     1801 attacgctga cggaagatgt agctaaagga gttgaaggtg ctgactttat ctataccgat
     1861 gtatgggtgt cgatgggtga agcaaaagaa aaatgggctg aacgcattgc attactgcgt
     1921 gattatcagg tgaacagcaa gatgatgcag ttgaccggta acccggaggt caaattcctc
     1981 cattgcctgc ccgcgtttca tgacgaccaa acgacgcttg gcaaaaaaat ggcggaagag
     2041 tttggcctac atggcggaat ggaagtgact gatgaggtct tcgaatctgc cgccagcatt
     2101 gtatttgatc aggcggaaaa ccgcatgcat accatcaaag cggtgatggt cgcgacgctc
     2161 agtaaattaa ataattaata tctctataaa
//
LOCUS       DAZPAB010000347         2187 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZPAB010000347 DAZPAB010000000
VERSION     DAZPAB010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.350"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(220..384)
                     /locus_tag="ACHUPG_004177"
     CDS             complement(220..384)
                     /locus_tag="ACHUPG_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863531.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1286..1570
                     /locus_tag="ACHUPG_004178"
     CDS             1286..1570
                     /locus_tag="ACHUPG_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR0863532.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1558..2043
                     /locus_tag="ACHUPG_004179"
     CDS             1558..2043
                     /locus_tag="ACHUPG_004179"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0863533.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          565 a          462 c          620 g          540 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc
      481 gaaaatgtgc tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc
      541 ggcatccggc atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc
      601 tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg
      661 atgcgagaca caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg
      721 agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc
      781 catgagtcag ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg
      841 ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt
      901 taccaatcga agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt
      961 gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga
     1021 attcgtcacg actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg
     1081 gttcaacgac aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg
     1141 ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta
     1201 aatgccgctg acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg
     1261 tcaatatgct attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca
     1321 gctcaatctc agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct
     1381 tcataagtcg cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat
     1441 tctcgaccgg cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct
     1501 tgatgctccg gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg
     1561 ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc
     1621 agtgaagctg tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga
     1681 gccacccgga cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca
     1741 ctggccactg gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca
     1801 gaccccattc ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa
     1861 gggcttggcg cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat
     1921 atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt
     1981 catcatggtt ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa
     2041 tagggagtaa tgccgatcag ttaaggatca gttgaccgat ccagtggctg tgtaagaatc
     2101 cggaaacgct cactcgtttc cggatttttc tgtgcacagt aaaagaccac ctcctgagga
     2161 ggtggctttt cagaatgccc cctataa
//
LOCUS       DAZPAB010000348         2183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZPAB010000348 DAZPAB010000000
VERSION     DAZPAB010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.351"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..203
                     /locus_tag="ACHUPG_004180"
     CDS             <1..203
                     /locus_tag="ACHUPG_004180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863534.1"
                     /translation="RAGVPGSDEETRADFENRRRNSVARNARNILEAIRGEILSTVEN
                     VVDVYVTHNPKKTEQKAGVSIH"
     gene            293..694
                     /locus_tag="ACHUPG_004181"
     CDS             293..694
                     /locus_tag="ACHUPG_004181"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863535.1"
                     /translation="MNGNTTFTVADKEYDPPYPEYVISWQTLKPVSLHVSVTLKKSDY
                     LPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSR
                     DGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            696..1301
                     /locus_tag="ACHUPG_004182"
     CDS             696..1301
                     /locus_tag="ACHUPG_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HFR0863536.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1288..>2183
                     /locus_tag="ACHUPG_004183"
     CDS             1288..>2183
                     /locus_tag="ACHUPG_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0863537.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          531 a          507 c          609 g          536 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtatccat taacacccgg ttcgctttat gttggcgtgt acggcggcag
      241 cccggcagat atcgcggcgg ccatctggcg taaggctccg ccgggtattg atatgaatgg
      301 caacacaacg ttcactgttg cagataagga gtacgatccg ccgtatcctg aatacgtgat
      361 cagctggcag acactcaaac ccgtcagtct gcatgtcagt gtgacgctga aaaaaagtga
      421 ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg catttaatgg
      481 tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc gctactatgc
      541 cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg tgagccgtga
      601 tggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg ttctggatgt
      661 gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct gccactgtgc
      721 ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt aacgcagcgc
      781 tttcccccga cagttttatc agtgattttt atggtcttat ctggaacatc gataccgcag
      841 aaaagtatgg tcttgatgtc tggggaaaga ttgtgggtgt cagtcgctgg ctgacggtaa
      901 aggacgattt taattacctg ggcttcagcg agtccaggat ggacaccccg gtaatggatg
      961 atccctgtcc gtttaatcag gcaccgtttt acaacggaaa atcggatacc cggactgttg
     1021 acctgtctga tgctgtatac cggcggctga tactgatgaa agccatgtcg aacattactg
     1081 actgttccgt tccggatatt aacctgatgc tgagatttat gttcggaaaa aaacgccggg
     1141 cttatgttct gaataatggc gggctgagga tgagttacgt ctttgagtcc gcgctctcgt
     1201 tggcagaact ggcgattatc cagtcgtcgg gtgcactgcc atccccgccg ggtgtttatg
     1261 tttcagtagt tttaaaggag tcccgtaatg aaggccagtg ataaaccccg ccagctggcg
     1321 gtcccctttg cgagtaccgg agataaaaac cgtatcccgg acaaggcgac acagcagacc
     1381 agagagagcg gtaatgctgc gtatgattca ggttttcctc cggtgaccat gacagcggtc
     1441 tcagcgggag gtataccgcc acacggcaag gattttaacg gtctgatgta cgatattacc
     1501 gcagcaatac ggttcgccca ggctggcggt ttgtacacgt ataatgccgg ttttgcgggg
     1561 gccattggtg gatatgcaaa aggagccatt ctcgccggag tcgcaacaac agcggtctgg
     1621 ctgaatacca cggacgataa cctgaccgat cctgaaggct ccgacagtgc gggctgggta
     1681 aatcttcttg aggatccgaa aaggatattc ctgcggcaga agaacaatct gtcagacctt
     1741 cagaataaag ggacggcacg ggataatctt caggtttaca gtaaagagca gtcagatcaa
     1801 cgctatgttc atcgggaagg cgataaaata accggagagc tgaaaatccg tggtgttaat
     1861 gcgctgagga ttttcaacga agcttttggc ctgatttttc gtcgttcgga agagtgcctg
     1921 caccttattc ccaccagtga aggtcagggg gaaaatggcg atattggtcc cctgcgcccg
     1981 ttcaccatta atttgcggac gggtgaaata tccatgtcgc ataaagtgtc tgttggcggt
     2041 ggttcgcagg tcaatggtgc gctgggtatc ggcgttcaga acgccctggg ggggaattca
     2101 attgttcttg gtgataatga caccggattt aaacagaatg gagacggtat tctggatgtt
     2161 tatgctaata gtcagcgtgt att
//
LOCUS       DAZPAB010000349         2176 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZPAB010000349 DAZPAB010000000
VERSION     DAZPAB010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.352"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            82..390
                     /locus_tag="ACHUPG_004184"
                     /pseudo
     CDS             82..390
                     /locus_tag="ACHUPG_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
     gene            362..>2176
                     /locus_tag="ACHUPG_004185"
     CDS             362..>2176
                     /locus_tag="ACHUPG_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR0863538.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIESSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQR"
BASE COUNT          563 a          476 c          604 g          533 t
ORIGIN      
        1 gcagccttca gggaaaaata tcatcagatg gctgcttaaa aaaagaacaa atggtagtgt
       61 ccgctattgc cagtacacct caaactactc attgccagat gggaactgac cgcagaacag
      121 gctgtcgcac agcagttaaa aaatcaggtg tcaaaaggaa atcttatcga taccggattt
      181 tgtatttttg cccttagtaa gctggcgatg gctttatcca gtacgcttga ctccattcct
      241 ttgtccatgc aacgacagtt cccggattta acgccgcgtc atattgatca tctgaaaatc
      301 cttattgcta agggagcgaa tcagtgcgcg cgtgcggggg ataaattacc ggatttactc
      361 gatgaatata tcagaacaac aactgagtaa tatgatggtg gcagtcacta tcgcactgca
      421 gccactggtc agggtattgc cggtgacggc tgtagaatgg gccgatcaaa attattacct
      481 gcctaaagaa tcgtcatacg gtgaggggga atggaaaacg ctgcagtttc aggtcgccat
      541 catgaactgc atgggcaatg acgagattcg tacagttaac ctgattaaat ctgcccgtgt
      601 tggttataca aaaatggtgc ttggtgtgat cgggtatttt attgagcaca aatcccgcaa
      661 cactcttctt tttcagccga cggactcagc tgctgaagat tttatgaagt ctcacgtgga
      721 agcaacaatt cgtgatgttc cttgcctgaa aaaactttct ccctggctgg gccggaaaca
      781 ccgggataat acgctcacac tgaagcgttt ttcctctggt gtggggttct ggtgtttggg
      841 tggtgctgct gccaaaaact accgtgaaaa atctgtcgat gtggtctgct atgacgaact
      901 ctcctcgttt gaaccggatg tggaaaaaga aggttcgcca acactgcttg gcgataaacg
      961 tattgaaagt tcggtatggc caaaatccat acgcggttca acgccaaaaa ttaaaggctc
     1021 ctgtcagatt gaaaaagctg cgaatgaatc tgcgcatttc atgcggtttt atgtcccttg
     1081 ccctcattgc ggggaggagc agtatctgaa gtttggcgat gatgcgacgt cgtttggcct
     1141 gaaatgggag aagggtaaac cggaaacggt gtattacctg tgtgaacata atggctgcgt
     1201 gatccgtcag tctgaacttg atcagaccgg ggggcgctgg atttgtgaca ataccgggat
     1261 gtggacgtgt gacggtctga cgtttttcag tgcttcgggt aatgaaattc cgccgccgcg
     1321 ctccatcact ttccatatat ggacggcgta cagtccgttc accaccaggg tacagatagt
     1381 ctgtgactgg ctggatgcac tgaaagatcc caacggcgtg aaaacttttg tgaacaccac
     1441 gctgggcgag acctgggaag aggccgtggg cgaaaaactc gatcaccagg tactgatgga
     1501 taaggttgtg ccttacacgg caacggtgcc tgtccgggtg gtttatctga cggcgggcat
     1561 tgactcgcag cgaaaccgtt ttgagatgta tgtctggggg tgggctccgg gagaggaagc
     1621 ctttctggtg gataaaatca tcatcatggg acgtcctgat gaggaagaga cgctgttacg
     1681 tgtggatgcg gcgatcaata aaaaataccg ccatgcagac ggtaccgaaa tgaccatttc
     1741 ccgtgtctgc tgggatatcg gggggatcga tggcgaaatc gtgtatcaga ggtcaaaaaa
     1801 acacggtatt ttccgggtac tgccggtaaa aggtgcctct gtctatggca agccggtgat
     1861 caccatgccg aaaacccgca atcagcgggg tgtgtatctg tgtgaggtgg ggacggatac
     1921 cgcaaaagaa attctctatg cccgaatgaa ggccgatccc acccctgtga atgaggccac
     1981 atcgtatgcc atccgctttc ctgataatcc ggagattttt tcgcagacag aggcacaaca
     2041 actggtggcg gaggaactgg tggaaaagtg ggaaaaagga aagatgcgtc tgttgtggga
     2101 taacaaaaag cggcgtaacg aagcgctgga ctgcctggtg tatgcctatg cggcattacg
     2161 tgtgtccgtg caacgc
//
LOCUS       DAZPAB010000350         2172 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZPAB010000350 DAZPAB010000000
VERSION     DAZPAB010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.353"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            140..1222
                     /locus_tag="ACHUPG_004186"
     CDS             140..1222
                     /locus_tag="ACHUPG_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFR0863539.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(1711..2172)
                     /locus_tag="ACHUPG_004187"
                     /pseudo
     CDS             complement(1711..2172)
                     /locus_tag="ACHUPG_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          641 a          447 c          434 g          650 t
ORIGIN      
        1 tcagataaaa atagggatct acttcacaat ttaaatgaga aactaaaact tacatcttga
       61 aataatctca ttgattggat gaatatttgt agtgcagtga catcattttt aaataatagt
      121 tcaaataaaa gggctcacga tgaaaaaatt aacagtggca atttctgctg tagctgcatc
      181 agtactgatg gcgatgtctg ctcaggcagc agagatttac aataaagaca gtaacaagct
      241 ggatctgtac ggaaaagtta atgctaagca ctacttctct tctaacgatg cagatgatgg
      301 cgacacaact tatgttcgtc tgggcttcaa aggcgaaact caaatcaacg atcaactgac
      361 tggtttcggt cagtgggaat atgaattcaa aggcaaccgt gctgaatctc aaggttcttc
      421 caaagataaa acccgtcttg ccttcgctgg cctgaaattc ggtgactacg gctccatcga
      481 ttatggccgt aactacggtg tagcatacga tatcggtgca tggaccgacg ttctgccaga
      541 attcggtggc gatacctgga cccaaacaga tgtgttcatg actggtcgca ccacaggtgt
      601 tgcaacctat cgtaacaacg acttctttgg tctggttgat ggtctgaact ttgctgctca
      661 gtatcagggt aaaaatgacc gcactgacgt aactgaagct aacggtgatg gtttcggttt
      721 ctccactact tatgagtatg aaggattcgg tgtaggtgca acctatgcta aatctgaccg
      781 cactaatgat caggttatct acggtaacaa cagcctgaat gcatctggtc aaaatgctga
      841 agtatgggca gctggtctga aatatgatgc gaacaacatc tatctggcta ccacctattc
      901 tgaaacccag aacatgactg tttttggtaa taaccatatt gccaacaaag cacaaaactt
      961 cgaagtagtt gcacaatatc agttcgactt cggtctgcgt ccgtccgttg cttacctgca
     1021 atctaaagga aaagacttgg gtgcgtgggg tgatcaggac ctgattgaat atattgatgt
     1081 aggtgcaacc tattacttca acaaaaatat gtccactttt gttgattaca aaatcaacct
     1141 gattgataag agcgatttca cgaaagcatc tggcgttgct accgatgata tcgttgctgt
     1201 aggattggtt taccagttct aatctgatta ctcaaagata tgttgtggga ggctttgcct
     1261 ccccaacata taagtggcgc tctcaagcca ctttccttag aagcactatc ttgcttctga
     1321 ctatataaat taccctttat tttctgggac gtttcaacgc cccattttta ataatactca
     1381 gtgaacaatt aacgtatcaa ttagatttaa taacaacgat atccgtacct aacctgatat
     1441 ctaatgtcat aagcatccct ttaattatcc cctcaacctc ttgtaaattt cccactcttg
     1501 agctccacat agcccaaacg ccggatgcaa aaaaaatcat acgtgcatct ctgaaattgt
     1561 gatggaggaa gtgagtatca attatatgtg cttataaacg ttcccgattg gcacagaagt
     1621 caccggattg ttcaggctcc aataggttga gtatgtcgta tttattttcc aaaataaaaa
     1681 tcgatgctgt tgctccttaa taaaatactt ttactctctg gacgcttttc ttagctctct
     1741 tggtgttata ttaaaacgat tatggaacct ttcagtaaaa cgagaaggac acttgtaacc
     1801 attttccctg gcaatctcgc tgatgggttt tactgtcgtt tgtatagcag gacaacgcat
     1861 tatttaacct cacgtcgtcc aatatacttt gaaaacttat tccctcgctt gccagacggc
     1921 gatgcaatgt ggaaacagaa atgtagagat atctcgctac cttgtttgct gtccattttg
     1981 ttctaggttc ggatagcatt aggttataac aacgacttat taatgattgt ttactatctg
     2041 ataaaagtaa acaattaaca tgatgtgctc ctaatgaaag tagaacgccc attgctaagt
     2101 gttcctgaat ttgcgttgag aagcctcggg aaatagatgt ttttagttgc tcccaacaat
     2161 atattaactc gg
//
LOCUS       DAZPAB010000351         2167 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZPAB010000351 DAZPAB010000000
VERSION     DAZPAB010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.354"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..590)
                     /locus_tag="ACHUPG_004188"
     CDS             complement(<1..590)
                     /locus_tag="ACHUPG_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HFR0863540.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAHDLFFIVLSGLSGPFIIFIIASSVFKYLHLSKGV"
     gene            complement(603..1520)
                     /locus_tag="ACHUPG_004189"
     CDS             complement(603..1520)
                     /locus_tag="ACHUPG_004189"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR0863541.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRTERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTIVFGNPVRGYPSMLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPRYLIKSRK"
     gene            complement(1517..1879)
                     /locus_tag="ACHUPG_004190"
     CDS             complement(1517..1879)
                     /locus_tag="ACHUPG_004190"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol glucosyl transferase"
                     /protein_id="HFR0863542.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
BASE COUNT          687 a          438 c          398 g          644 t
ORIGIN      
        1 attactcctt ttgataagtg taaatattta aaaacagatg atgcaattat gaatataata
       61 aatggaccgc tcaatccaga taatactata aaaaataaat catgtgcttt ccataatttt
      121 gacttggatt cttcagatat tattatcatt gcaacataca atgataaata ccagtgagca
      181 tttgttatat tggcttggac ttcatcaagc ccgggcatgc aaattaaata tacagagatg
      241 aagatttttg atgtggtgct taggaattta aatctatcgg taaataagaa ccatataatt
      301 atagctctaa ttattattcc aaaaaagttt gataataatg gtgcgtaaat tggattaata
      361 aatgtcgtcg caccaacaat gagtgttgat actgtttgaa aataaccatt tcttggggtt
      421 atgagtgaac tgaaaaaacc ttgttcgtat gcatctttat accaaaaaac tgcatcctca
      481 gcccaaaact gggcatttgt caaaatgtca atgcgtctaa gccaaatcaa agtaaatgca
      541 agtataaaaa ttgatatctt gtgccaattg cgcattatga atatcttcat aagatcatcc
      601 tgttattttc tgcttttaat taagtaccgt ggtcttgatt ttgtttctaa ataaattcta
      661 cctatatatt caccgagaac accaatcccg atcagttgca ctccacccag gaaaagtatt
      721 gatacaagca tggagggata cccgcgtact gggttaccaa agacaatggt gtctataatc
      781 atccatgcac catataaaaa tgaaatgctt gcaacaaaca agcctatata agtccatacg
      841 cgaagaggaa aggttgaaaa acttgtgata ccttccagtg ccagattcca taatttccag
      901 ccattaaatt ttgagatgcc agcaacacgc tctgtgcgta catattcaac gacatctgtc
      961 tgaccaccaa cccagctcag tatgcctttc atgaaaagat tgcgctcagg caatagttta
     1021 atgttctcca caacctcacg agacatgagt cgaaaatctc cgacattttc ctctattttt
     1081 ggggtgctta ttttgttgtg taatttatag aaccactcag ctgtcttacg tttcagccgt
     1141 ccatcagttg agcggtctga acgtttagca agcaccatgt cagcacctgc ctgccacttt
     1201 tcaataagat gagggataac ctcaatcggg tcttgcaggt caacatcaat tggaatcacg
     1261 gcatcgccgg ttgtatggtc taaccctgca aacaatgctg gttctttacc aaagttgcgt
     1321 gtaaatgaca gcggaacaac tagcggatca gaaacagcca gagcgttaat gattgactcc
     1381 gtagcgtctt tgcttccgtc atttatgaaa acaatttcta cttcatatgg cttcaactct
     1441 tcgaattcac gtaccgtttt ataaaaaatt ggtatcgctt cttcttcatt gaagacagga
     1501 acgacaagag atatcttcat ttcgcatccc taaagacaat gaactttgaa tagacgaaac
     1561 cgcacaccaa gctgatggcg gagaaggtaa caagagtgac aattggagga agtgaacatc
     1621 tatcagcaac ccatccaaca gtagcactca gtgttcccat gaacccgaca tataacatgt
     1681 agcgcatcgt tgtagttgat gctttgaatg tgaattttgc attcgcaaag aagctaaaac
     1741 tcacagccac aacgaaacct gcgaagtttg ccatagcctg attggtatgc gcggcataga
     1801 tacatacacc aaaaaccacc cagtgtataa gtgtgttcag cacgcctata gatgtgtact
     1861 ttgcaaatag ctttaacatt tcttctatca gctaataatc aaaggcatga agtctatcat
     1921 ccaagtctca atcgatcgat acttgcggta gttgatgaga aaaactctgg cacacaaatc
     1981 tttgcactgg attgcaaggc tttgtgctct tctgtgaatg tgtggctata tgtttgaaga
     2041 tcgttgtgcc gtatttgtga catatatatg gcaacatcat tcatcaactt tctgtttgtg
     2101 ccatcaacta tatcttagtg aatgcggtta atgcttgcta aaacagatag ttatgattgg
     2161 tgctaca
//
LOCUS       DAZPAB010000352         2164 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZPAB010000352 DAZPAB010000000
VERSION     DAZPAB010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.355"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            48..122
                     /gene="tnaC"
                     /locus_tag="ACHUPG_004191"
     CDS             48..122
                     /gene="tnaC"
                     /locus_tag="ACHUPG_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFR0863543.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            343..1758
                     /gene="tnaA"
                     /locus_tag="ACHUPG_004192"
     CDS             343..1758
                     /gene="tnaA"
                     /locus_tag="ACHUPG_004192"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFR0863544.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1849..>2164
                     /locus_tag="ACHUPG_004193"
     CDS             1849..>2164
                     /locus_tag="ACHUPG_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR0863545.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYI"
BASE COUNT          587 a          482 c          511 g          584 t
ORIGIN      
        1 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
       61 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      121 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
      181 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      241 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
      301 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      361 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      421 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      481 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      541 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      601 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      661 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      721 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      781 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      841 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      901 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
      961 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1021 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1081 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1141 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1201 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1261 tatggcggct tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1321 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1381 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1441 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1501 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1561 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1621 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
     1681 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1741 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1801 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1861 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1921 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     1981 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2041 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
     2101 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2161 atct
//
LOCUS       DAZPAB010000353         2162 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZPAB010000353 DAZPAB010000000
VERSION     DAZPAB010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.356"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(1250..1402)
                     /locus_tag="ACHUPG_004194"
                     /pseudo
     CDS             complement(1250..1402)
                     /locus_tag="ACHUPG_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1458..2151
                     /gene="ospZ"
                     /locus_tag="ACHUPG_004195"
                     /pseudo
     CDS             1458..2151
                     /gene="ospZ"
                     /locus_tag="ACHUPG_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
BASE COUNT          707 a          384 c          392 g          679 t
ORIGIN      
        1 ttgaactttt gtaaaaataa aaaaatacct cattgtgcga ataaagtaac gggggccata
       61 gatttctcta cctacctttt ggtgttcata cctgaggctg atagtttcag gattccgcga
      121 caattctact caccttcttg ttctctcgcc aatatgaaat caatcagaaa agcgttccat
      181 aagaacaacg ataagacaat aaattgtcat cacggctgca atagccaacg tgcctttcag
      241 taccaacatg acaacctcct gtatttgacg taacgtgtgc tgataaatat gtatttgtat
      301 ctcaactgac tcaatacaac acttaagtat agtttttcag attttgtttt tcccgtgaaa
      361 tattatggaa atggcagaag caatacattg aattccgatt cgagtgaata cggctagcgg
      421 gcacctcact ttagcactga agccatcaac ttttatttga cttatttcaa tggaaagctt
      481 tctcactatc agtacaggta attcccatcc ttttaaagtc gagagtcaga ctgcgactaa
      541 tacagttaaa gtgaccaaac tcagttatcc tctacacatt ttatctcatg tttacttgga
      601 caattcattg gtgcttttat aaaagaataa atattctact aatttaatgc atcacctaaa
      661 atagtatttt ttataataga actgtatcta tagtatatta ttaaagctta attatttatt
      721 atgctttaat gtggttgtcc gattaaggac taatccttga aaacgccgta tagggcgaga
      781 gttcactcta ttgcgataac gtaaccgtac aatattgtgc gtttcccaaa ttgaccacta
      841 ctgaactatt ctgggattta aatgcaataa aatggttggt tgaaggtcgt ggtgatgatg
      901 ttagtccttt tgagaatatt ccagtgccag atcttactta taataaagag gcttggcagt
      961 ttggataagc tattgtcctt attccactaa tacaaacttt ttgtaaagta tattaaatat
     1021 acttcatttc atactgaaat ccccggaagt attcaatcaa acaattacac cataaaagga
     1081 agagggagcc ccaattaagt tatcaagcat gtttatgata cctgcagggt ataaattttc
     1141 tggctgcaag cagacacata taaaacgtct ttattgatcc tacccacata atatggacgc
     1201 agtcgtaagt gaggctctgt gagcctaccc acgtaatatg gacacaggcc taagcgaggt
     1261 tctggttttc aaattgttcc ggactgaggc cgccacacca acggtgccgt cgccagcgat
     1321 tgtaattgca ctcgatataa ttaaacactg ttgcccgcac tatttcccga tagataaagt
     1381 gttctccgtg gagacattcc acttaggaac aattatattg ttcatggtaa tgtttatatt
     1441 tgagtataga gattaaaatg attagtccca tcaagaatat taagaacgta tttccaataa
     1501 atactgcaaa tactgaatat atagtgagaa atatttatcc tagagttgaa catggttact
     1561 ttaatgaatc accaaacata tatgacaaga agtatatctc tggtataacc agaagtatgg
     1621 cacaattaaa aatagaggag tttattaatg agaaatccag acgacttaat tatatgaaga
     1681 caatgtattc tccatgtccg gaagattttc agcctatttc cagagccgaa gccagtacac
     1741 cggaaggaag ctggttgaca gttatatctg gaaaacgtcc tatggggcaa ttttctgtgg
     1801 atagtttgta ccatcctgac ttacatgcat tatgtgagct cccagagatt agttgtaaga
     1861 tcttctctaa agaaaatagt gattttttgt atataattgt tgtattcaga aatgattctt
     1921 cccaaggaga actacgggca aatcggttta tagaactata tgatataaaa agagagatta
     1981 tgcaagtgtt aagagatgag tcgccagaga ttaaagtcta ttaaatctga gattattatt
     2041 gctcgtgaaa tgggagaact ttttagttat gcatcagagg aaatagatag ctacataaaa
     2101 caaatgaatg acaggctttc tcaaatcaag gctcgaatgc ctgttactta atgtatatta
     2161 at
//
LOCUS       DAZPAB010000354         2138 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZPAB010000354 DAZPAB010000000
VERSION     DAZPAB010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.358"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            310..1500
                     /locus_tag="ACHUPG_004196"
     CDS             310..1500
                     /locus_tag="ACHUPG_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HFR0863546.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(1890..>2138)
                     /locus_tag="ACHUPG_004197"
     CDS             complement(1890..>2138)
                     /locus_tag="ACHUPG_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HFR0863547.1"
                     /translation="MSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKI
                     YPIVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
BASE COUNT          598 a          444 c          474 g          622 t
ORIGIN      
        1 cagtaccacc gatagtatcg atatcaaatc agcctgcggc cgcgataact ttcgtaacat
       61 tctgccagcg gctttgttga ataaatcagg ttcaagataa tcgtctctgc atcaggttag
      121 agtttcaggc aatacatcct ctttctggca tacctgccac tctcgcacca tggtcatagc
      181 ctttacgatc tgcatactca ccagaccaga gcccgctccc ttttaggctg aggaatcccc
      241 agaaaagaag acaggcatag agttttgtga tctactgatt gcgctaacaa attcaatgag
      301 atcacctcta tgcctgcccg taaagtatgc cagacttttt tccgtaatgc tttagcttcc
      361 acgcatcagt atcgacaaaa tgccattatt gattctgctg ctgctttagt cggtggcgca
      421 tctctttctc ttaccagcat tggccggcat ttacccggtc ctgctcgcgt gaaagataaa
      481 ataaaacgcg ttgactgtct gttgggtaat aaacgacttc ataatgatat ccctctgata
      541 ttcaaaaata ttacatccat gatgacaaat aaactgtcgt ggtgtgttat cgctgttgac
      601 tggagcggtt acccttttca ggaatatcat gtccttcgtg ccagcctcct ctgcgacggt
      661 cgctctatcc ccctgatgag tcaggtcttc ccgtcaaaaa aaaataacga ggcggtggaa
      721 atcgcttttc tggatgcgct ttccggggcc atttctcccc ggactcgtgt tgttattgtt
      781 actgatgctg gttttcaaag tgcatggttc cgtcatatca aatcacaggg atgggatttt
      841 attggtcgta tcaggggggt agtgaagttc aggctggata gtgacaaaga taagtggctg
      901 gacataaaaa tgtgcagggg gagttcagag gcaaaatacc tggggacggg aaccctggcc
      961 cggaaaaaac gctcgcagtg tgaaggacat ttttatcttt ataaacattc acctaaaggc
     1021 agaaaaagca ggcgtgccag agggaggccg ggcttaccaa caacggaaaa agaacaaaaa
     1081 gccgcaggca gagaaccatg gttaatattc agcaatacag ctgagtttaa tgccaaaaaa
     1141 atcatgaaac tgtacagtcg aagaatgcag atagagcaaa actttcggga tgaaaaaagt
     1201 gaacgctttg gttttggcct tcgggcgagc agaagtcgga ggggagagcg ttttctggta
     1261 ttaagtctac tggttacact ggccagtatc gtactctggc tacttggtta tcattttgaa
     1321 aataaaggat ttcatttaaa gtatcaggct aatagtctca aaaaacgaag agtattgtct
     1381 tttctgacgc tggctgaaaa cgtcctgaga tttaaccctg aacttctacg acgagcacaa
     1441 cctgaaaaga tattgtgccg gttggccagc acttaccgaa gtatggtgct ggcttactga
     1501 tgaaaatttt ctggggatcc ctcagccttt cggggaggaa tgagggctct gatattcttt
     1561 tgccgtagtt catcgtgaaa ccgtcggatg tcatttggcc tgagtgaccc atgcctccca
     1621 aaagcagagt cgctagtgtt atgtaattag gcggttaaac ttcaattctc agtgttaagt
     1681 ttgggtaaac gcttgtaaat ggtattgcta attttttaat tgactaatat tagaaacggt
     1741 tagtgtgatt gtatatagat atttgagtgt aaggtattta aaggggcatt cagcccctcc
     1801 gtctatttgt gattgcatcc attgatgtgt gtattatttt gagcttgaaa aagggaggcg
     1861 ctctttaata ataaataaat ctatcaaagc taaatattaa atggaagcca cccttttttc
     1921 tctgatagtt ttattatcac caaaccattt atttcagcta tcgaatctgg attcggccag
     1981 gaaggtaaac tcttcacaat tggataaatc ttctctattt gttctttatt tgctggtttg
     2041 caattacaga ttccatagta attcataaat gcaaccatcc taaaatagtt gccaccatcc
     2101 caccaaaaaa aagattttcc aaaagtgtct gcgctcat
//
LOCUS       DAZPAB010000355         2123 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZPAB010000355 DAZPAB010000000
VERSION     DAZPAB010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.359"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..126)
                     /locus_tag="ACHUPG_004198"
     CDS             complement(<1..126)
                     /locus_tag="ACHUPG_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HFR0863548.1"
                     /translation="MVVGCSSKENALCHPQPKPPAPPAWAMMPPSNSLQLLDETFS"
     gene            complement(68..388)
                     /locus_tag="ACHUPG_004199"
                     /pseudo
     CDS             complement(68..388)
                     /locus_tag="ACHUPG_004199"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            complement(385..918)
                     /locus_tag="ACHUPG_004200"
     CDS             complement(385..918)
                     /locus_tag="ACHUPG_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR0863549.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(984..1613)
                     /locus_tag="ACHUPG_004201"
     CDS             complement(984..1613)
                     /locus_tag="ACHUPG_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863550.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1617..1832)
                     /locus_tag="ACHUPG_004202"
     CDS             complement(1617..1832)
                     /locus_tag="ACHUPG_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR0863551.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          544 a          563 c          468 g          548 t
ORIGIN      
        1 cgagaacgtt tcgtccagca actggagaga gttcgacggt ggcatcattg cccatgccgg
       61 aggcgctgga ggtttcggct gaggatggca cagggcattt tcctttgacg agcacccgac
      121 caccattatc aagcttgcgc cgaagagcat cattttcagc tttcgcatca gctaactcct
      181 tcgtgtattt agcatcgagt gcatcagcat cacgctggcg ctgctgcatg tcagtaatgg
      241 ttgcgttcgc cagcttcagt tctccggcgt ttttgtcgcg ctgctctttg taggtaatgg
      301 cgttatcacg gtaatgatta acagcccatg acaggcagac gatgatgcag ataaccagag
      361 cggagataat cgcggtgact ctgctcattg atctatcccc caacaggcta atgcgctttc
      421 ctggtcacga cgaataacct gtccatagca gttatttgaa cgtatgcggc aatcgcgccc
      481 aacatctttt atccaccagc gaatcgcctc gcatgcgccc ttacgatcac cggcattcag
      541 ccgcttataa aacgtcgacg ggaaacactt accggggcca atgttatagg ggcaaaatga
      601 cgctataccc gctttctggg gttcggtcag aggtacttta atattgcgct ccacccatgc
      661 cagcgcctta tcacgttcaa tagcgttaac ctggtcacat ttttccttcg acagcttcat
      721 tcccggtatg acaggcttac catccaccat tgtggcacca cgacagatgg tccatatgcc
      781 agaaccatca cggtatgccg ttgtgtgatt accctctttt tcgttcagaa actggtcaag
      841 tatctgggga gcagacgcgc ctgcggcaat cagcgccaga acagcagccg acaggccgta
      901 tttgattttt gcgctcatgg atatttatca ggatttatcg gcaacaaata acgagccaac
      961 ttatatacgc cttttaagat aagtcagccc cggctggatc cagtcatcag gctctttctt
     1021 aaagggggag tattgaaatc acgaagaaga gcctcccgca ccgcagcatc catgtctgca
     1081 ccactggcaa gtgcttcaat ctctgccgcc acctgcaaat accccatgca acggccaatg
     1141 cgtttcataa ctctggtgtt ttcgtctttc aggcatctga tagcgagatt aataattttc
     1201 aggcagtgat gatgcgatgc ccggacaggt tttccgttct cataaacgac gaactcaccc
     1261 tcttcacagg aaaccctgaa aggccgatgg ggttcctggt ctttatccgg tgcagacgca
     1321 ataaaatcac ttttatgctc ttcctctccg gcactgttaa ccgtaatgac gtactcaggc
     1381 cgcaggtaag ccgcctcttt ttctgacaat gcggccggta aaaacttttc ctgcaaaaac
     1441 ttcataaaag tctcgtgagc gactttgtgg cagtaatcgt aaatcgccgc ataatgctca
     1501 tcgcggcgtt tgttttcatc ttcagaactc accaacgccg acaatttttt attaagctca
     1561 gtgatttcat tttccaggtg attgaatcgc tgattcattt cttcatgctt cattatttac
     1621 tctccccgtg cagccttacg cttgtcttct cttattttga aatacagatt cgtcagataa
     1681 gtcagaagcc ccagaagcag acttcccagc acaccaatcg cagcccactg tgatggcctg
     1741 acctgatcca accactgcaa aaaccagtag ccggcactgc cggcggaggt gccgtaggca
     1801 atgcccgttg aaattttgtc catggatttc atagcctcac ctccgcaaat aacggatggc
     1861 gtagttttac actgagaaat gaaagggatt tgaaaagaaa aaaacgcaaa agcgggcgaa
     1921 acgatatata cagtaaggaa agcactctat ccaacaaacc acccacagtt aatcggaata
     1981 aaagcagagt gcttatgaat gatcgcacgc ccgaaggtta gtatttctgc acagcaattt
     2041 tgcaaaaaaa ggcgatcatt cataacttaa acgtctttca gtcactccgg gatttcccat
     2101 catcgcagac tgaaagactc tga
//
LOCUS       DAZPAB010000356         2092 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZPAB010000356 DAZPAB010000000
VERSION     DAZPAB010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.360"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="ACHUPG_004203"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="ACHUPG_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR0863552.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /gene="mcbR"
                     /locus_tag="ACHUPG_004204"
     CDS             1351..2016
                     /gene="mcbR"
                     /locus_tag="ACHUPG_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR0863553.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          577 a          444 c          559 g          512 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg tgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgcccatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc gacaccataa tt
//
LOCUS       DAZPAB010000357         2087 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZPAB010000357 DAZPAB010000000
VERSION     DAZPAB010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.361"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1..799
                     /locus_tag="ACHUPG_004205"
                     /pseudo
     CDS             1..799
                     /locus_tag="ACHUPG_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010972061.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            914..1255
                     /locus_tag="ACHUPG_004206"
                     /pseudo
     CDS             914..1255
                     /locus_tag="ACHUPG_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1238..2075
                     /locus_tag="ACHUPG_004207"
                     /pseudo
     CDS             1238..2075
                     /locus_tag="ACHUPG_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          515 a          505 c          631 g          436 t
ORIGIN      
        1 tctgggcctt gcccgtagca gttattttta tcaccgggct gcactgaaag ccggtgataa
       61 atacgccacg atacgtaaga tgctgacaga tatctttaac agtaattacc agtgttatgg
      121 ctatcgtcgc ctgcatgcga tgctcaggca tgaggggggg cggctatcag aaaaggttgt
      181 acgcagactt atggtggagg aacagcttgt cgtcagtcgt aaccgtcgtc gtcgctacag
      241 ctcatattgc ggagaaatcg gaccggctcc ggataacctt atcgccagag attttaaggc
      301 ggagcaacct aatcagaaat agctgacaga tatcacggag ttccagctcc ctgcaggtaa
      361 agtctggctg tcaccagtgg tggactgctt cgatggaaaa gttgtgagct ggtctctcag
      421 tacacgccct gatgctgaac tggtcaacac tatgctggat agcgctgtcg aaacgttaaa
      481 tgctggcgaa cgaccggtga tacacagtga cagaggtggg cattatcgct ggccaggctg
      541 gctggaaaga gtgaatgcag caggtcttat tcgctcaatg tcccgtaaag gatgttcacc
      601 tgataatgct gcatgcgaag gctttttcgg cagactgaaa acggaaatgt attatgggcg
      661 taaatggtcg ggcatcacgc cagaaaagtt catgcagcaa gtggatgctt acatcagatg
      721 gtataacgag cggcgtataa aattatcgct gggtgccgtc agccccaaaa tgtaccgcca
      781 acaatgcggg ctggaatgat aaagcagtcc aggaaatcgt ccgcatcccc ccttcaccat
      841 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttcctacacc acaaaggaaa
      901 ctctcatgct gcgcagtgga tggctgacgt tgccgacagg cgggaacttc gccagttcag
      961 gcagacaccg gaacagcgct tcacgcagga acaggagcat ctgcagccgc tgctgggtac
     1021 ggacttcgat atccgtcatg tctcctggga tggctatatc gaggttggcg ggaaccgtta
     1081 cagtgtgccg gaaagcctgt gtggtcagct ggtatcaata cggatctcgc tggatgatga
     1141 actgcggatc tacagtaatg agcaacaggt agcatcgcac cgtctctgtt cggctgcata
     1201 cggctggcag accgtgccgg agcatcacgc tccactctgg cagcaggctt cggagcgcat
     1261 ggccgaatgg ctgggggaga tacagaaacg ggtaatcact gtctgtgacc gggaagcgga
     1321 tatctggcat tacctgtatt ataaagtcag ccacggacag cgtggtgcgt gctgcacaga
     1381 atcgccggct ggaagaggca cccggcaagc tcttcgaact gccggaagtc ctggcaaccg
     1441 caggaagcca cacgctgaat gtgatgcaaa aaggcgggcg ggcagcccgt caggcccgga
     1501 tgttcatcag ttacagcgaa gtcagcataa aaaatcccga caacagcggc caggcgctcc
     1561 cgctcacgta tgtctgttgc cgggagcagg cagaggacgg tgcctgctgg catctgctga
     1621 cgtcaggaaa agcggcgagt gccgccgatg cacgacgtat tgtcagccat tacgagcgac
     1681 gctggctgac agaggaatac cacaaggcgt ggaaaagtgg tggtacatgg aatcgctgcg
     1741 aatgcagacc cgggataacc ttgagcgcat ggtggttatc ctggcgttta tagcggtacg
     1801 ggtgctgggc ctgcggcagg gaggtgtcag tgaagaaaca caaaatgaca gctgtgagaa
     1861 aatactgacg ccgacagagt ggaaactact gtgggttaag ctggaaggaa agccgcttcc
     1921 ggtgcaggca ccgaccctga aatgggcctg gggcgatggc atgacagcaa acgcacaggt
     1981 cgtcccggtt ggggcgtcat gtgggatggc tggtcagact tcaggatatg gttgagggtt
     2041 acctggtgat gaagtctctt gatcaggagc tctgatcaag agacagc
//
LOCUS       DAZPAB010000358         2075 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZPAB010000358 DAZPAB010000000
VERSION     DAZPAB010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.362"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            155..1132
                     /gene="intI2"
                     /locus_tag="ACHUPG_004208"
                     /pseudo
     CDS             155..1132
                     /gene="intI2"
                     /locus_tag="ACHUPG_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            complement(1320..1559)
                     /locus_tag="ACHUPG_004209"
     CDS             complement(1320..1559)
                     /locus_tag="ACHUPG_004209"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR0863554.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT          603 a          450 c          434 g          588 t
ORIGIN      
        1 tcacccaaag aactcataac attttaattt atttagcatt aaattaaaac tctaccggta
       61 aacaagcatc tctaggcgta aaaaacaggc acttttttaa atcccagcta gaataggctg
      121 tataggcaga cagttgcaag acaaggagcg ttttatgtct aacagtccat ttttaaattc
      181 tatacgcacg gatatgcgac aaaaaggtta tgcgctgaaa actgaaaaaa cttacctgca
      241 ctggattaag cgttttattc tgtttcacaa aaaacgtcat cctcagacca tgggcagtga
      301 agaggtcagg ctgtttttat ccagcttagc aaacagcaga catgtagcca taaacacgca
      361 gaaaatcgct ttaaatgccc tagctttttt gtacaacagg tttttacaac agccgttggg
      421 cgatattgat tatatccctg caagcaagcc tagacggcta ccctctgtta tctctgcaaa
      481 tgaagtgcaa cgcattttgc aggttatgga tactcgcaac caagttattt ttacgctgct
      541 gtatggtgca ggtttgcgca ttaatgaatg cttgcgtttg cgggttaaag attttgattt
      601 tgataatggc tgcatcactg tgcatgacgg taagggtggg aaaagcagaa acagcctact
      661 gcccacgcgc ctaatcccag caataaaata actcattgag caagcgcggc ttattcagca
      721 agacgacaac ttacaaggcg tagggccatc gctgcctttt gctttagatc acaaataccc
      781 ttctgcttat cgacaagcgg cgtggatgtt tgtctttccc tccagcacgc tctgcaacca
      841 cccgtataac ggcaaattat gccgccatca tctgcatgac tccgttgcgc gaaaggcatt
      901 gaaggcagcc gtacaaaaag caggcatcgt tagcaagcgt gtcacttgtc atacatttcg
      961 tcactcgttt gctacgcatc tattacaagc ggggcgtgat attcgcactg tgcaagaact
     1021 cttagggcat aacgatgtta agaccacgca aatctatacg catgtgttgg gtcagcattt
     1081 tgccggcacc accagtcctg cggatggact gatgctactt atcaatcagt aaaagtagat
     1141 aaatatcttt atgtagccct gagtagcccc tgatactttt tatggtgaaa aagataaaca
     1201 atcatagagg ggataaatat gagccagttc taccattgtt cagataagtt taagcaagat
     1261 actgtcaatc acagtgacca ttcatggtca ttacacttcc tccagttgcc agacaatgcc
     1321 taacataaaa acgcaaaagc ccaaaagtca gagcttaggt gttctaggcg ttttctaagc
     1381 gctgaagaaa tacgtcttac cgtagattgc gtgcgtttct gaagcacatt ttccttatac
     1441 atggctttat cccaggcagc ttcatcaagc tcttggagta acaagccagt aataatgcga
     1501 cactctcggg tttgtagcga gccgctggtg agatcggagt tatagcggaa aggggtcatt
     1561 tgtaattatt tgtgggtgga ggatagttaa cttattattg tagttggaag aggctgcttt
     1621 ccaaacatta gcacctcgta atgcttgtta ctgtatatta atttatgctc ttagcgcatg
     1681 ttaatgggct attacaatgc ataatgtcca catcaagata actgtggctt aacacctgta
     1741 caagcgaaaa aaatcaatca cctgtccgga aatacgggat cactgtaaag tgttggtgag
     1801 gcagtctagg agggggattt aatttgggat actgatgaaa ggattatgat cagttggata
     1861 taggatcgta ctaagctgag cggcaggtta gctagattag ttaaccagat aagtgaaatc
     1921 tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc tacacccagt
     1981 tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt ccacccctcc
     2041 cagttcccaa ctattttgtc cgcccacagc tgccg
//
LOCUS       DAZPAB010000359         2071 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZPAB010000359 DAZPAB010000000
VERSION     DAZPAB010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.363"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..259)
                     /locus_tag="ACHUPG_004210"
     CDS             complement(<1..259)
                     /locus_tag="ACHUPG_004210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863555.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARC"
     gene            complement(1012..1410)
                     /locus_tag="ACHUPG_004211"
                     /pseudo
     CDS             complement(1012..1410)
                     /locus_tag="ACHUPG_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
     gene            complement(1413..1779)
                     /locus_tag="ACHUPG_004212"
                     /pseudo
     CDS             complement(1413..1779)
                     /locus_tag="ACHUPG_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1781..2071)
                     /locus_tag="ACHUPG_004213"
                     /pseudo
     CDS             complement(1781..2071)
                     /locus_tag="ACHUPG_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          472 a          581 c          488 g          530 t
ORIGIN      
        1 tgcagcgggc aatttccgga tacccttcgt catcaacacg tacaaaccag aagaccagtt
       61 ttttgtttcc ggcatccaca aagaacggaa tattcaggtc tgcgcaacac tcaacggcat
      121 cgtcaaaatt atcaaagcgc agaacctcag cttcttcttc gtcaaaaaaa tcatcttcgt
      181 gaagttttac gacatagcgg aggagtttgc ttctttgagg tgcaggtttt cgtttgcgtg
      241 gtttagctga acgggccata taactacctg aaagacaatg atattgtctg tttttataac
      301 ggtaatcaca ggccatgaca agccgcagcc gtcaggccgc ctactcgggg cggtttagaa
      361 cgcttattta gtaccagatc ttacttataa taaaggggct tggcagtttg gataaactat
      421 tgtccttatt ccactaatac aaactttttg taaagtatat taaatatact tcatttcata
      481 ctgaaatccc cggaagtatt caatcaaaca attacaccat aaaaggaaga gggggcccca
      541 attaagttgt caagcatgtt tactgcgcaa tcgcttggtg ctccgtccct gtaccccgca
      601 ttggtactct ggcgctcatg gggcttccac ttgcgcttct cccttcacat cggggtgaca
      661 ggtccacgtt ccccacaaga gcctgaatcg acttcacgcc gcttttatgc cggatgccgc
      721 gcaggcagta aacatatccc acaggaacga ctccaccatt gttttgacat catctatatg
      781 ctttcgacac atcatcagcg gttcactttc gttcgtctct tcgaaccata cctgatcggc
      841 tcacgtccga ccttttccca tctcgctcac caccacatct cttaaatgca gcagcagtgg
      901 atggtttggt gcctgcgcct gcacgccgac accaaggggc cttccctcat ctcttgtgct
      961 gcatggctgc acagggactc acgtccccag gacgcgcctt cgtggcacac agtcatcggc
     1021 atagcaacag taagcaacgg caggtttcca ttgccagttt tctttgacag cagtacttcg
     1081 gcctcgctgg atactgtgat tccagtacca gcgatccttt ctggcttttc cactcaggta
     1141 gcgtttattc agatactggt cgaactcatt cagtatgatg ttcgacagca acggcgagat
     1201 cacaccgccc tgtggcatac tttcactgac cgcccggaag agaccaacat caacacggcg
     1261 ttttccacag cagtctcata aaacgtgtat cactgatcct gcggcagacg gctttcatca
     1321 gcagccggtg atgtactgtg tcgaagtagc tggacaggtc accttcaata acccggcgcc
     1381 ccgtgtttcc ccgcagtcgg tgagctgtaa tagtacgtga tgtctcccgc ccacttctgg
     1441 ttcgggccac tggcgtaaaa atcctgcgtc agcagatttt ctgatacagg caggccgtgt
     1501 gcacggtaac tgatcgggct gaacttctgg gaggcttttg cgctcagttc ctgacggcgc
     1561 agactggctg ccacggtttt tacgttaaag tggtaaccag agcacacagt tcatccgtca
     1621 ggcgtggggc accgtaacgc tggactgggt aaaagccttg cggacaacgc tgtcgcagtg
     1681 ctgacggaac tgcggacgtg tgtttatcct tgtccgccgc tgacaccacg tataccagcc
     1741 gctgcgggcc acccggagta cgcggcacat tgccttgatg acggtttacg cggtcgcctg
     1801 cggcaacggc tttccggctg acggtagtgt ggaccttttt accccggaga acaccggcaa
     1861 gccccataac cgccatgaga cgtgccacag tgcatctggc cactctgata ccttcccgta
     1921 acaactgatg ccagacttta cgcacagcgt atacctgatg attctcatcg tatacgcgca
     1981 gtatctcttt cttcagccag tcatcgcgct gcgcacgggc actgcgttta tcatgatgat
     2041 gtcgctgttg ctgacagtgg taatacgttg a
//
LOCUS       DAZPAB010000360         2069 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZPAB010000360 DAZPAB010000000
VERSION     DAZPAB010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.364"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..257)
                     /locus_tag="ACHUPG_004214"
     CDS             complement(<1..257)
                     /locus_tag="ACHUPG_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR0863556.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKA"
     gene            469..1260
                     /locus_tag="ACHUPG_004215"
     CDS             469..1260
                     /locus_tag="ACHUPG_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFR0863557.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            1270..>2069
                     /locus_tag="ACHUPG_004216"
     CDS             1270..>2069
                     /locus_tag="ACHUPG_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR0863558.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT          487 a          533 c          523 g          526 t
ORIGIN      
        1 gctttttgtg gcggaagatc tgagaaatca agggcctttt ctttttgtcg aatatcgttc
       61 caggtatatt ccagcacttc gataaataaa ttttctttat taccaaagtg gtgataaacc
      121 gtctgtttac tacaccctgc tgcctttaca atattatcta ctctggcccc ttcataacca
      181 tattcggcaa attcattaac cgcacatagc agcaattttt ccttcaggct ctgtacgttg
      241 ctggtatcaa tatacataac gcttccccac gacctcttcg tggtgactat cataacctga
      301 tttgatttca gcttcagaaa aaagggcaaa aacttgtact ttcatcaggt tatcaaatgt
      361 cctgtgatcg ccatcgcata tcaaccaact agatggttta tctttttatc ctccagtcgc
      421 aagctaatca tcaccaagga aacaaaattc aaaaaagaga gctattgtat gaaatccact
      481 cgtcctgttg ccgtgattac cggagccact cgcggtattg gaaagggctg tgcgcaggag
      541 cttgctcgtg gcgggtttaa cctgctgatt aacgatcgtc ccgatgccga cagcgtagaa
      601 aaactgcata tcacacaaca agaatgtttg gcggaaggcg tggaagtgat ttgcttccct
      661 gctgatgtcg gtgatctatc tctgcatgag gagatgctcg acgcggcgca aaaccagtgg
      721 gggcggctgg actgtctgct caataacgcg gggatttcag tgaaaaaacg cggtgacctc
      781 ctcgacctcg aaccagacag cttcgatcaa aacattgcca ttaacacccg cgcgccgttc
      841 ttccttgctc aggcattcag taagcgctta ctggcacagc cgaaaccgga agcggagctg
      901 ccgcaccgct cgattatttt cgtcagttcc atcaacgcca tcatgctggc gatgaatcgc
      961 ggcgaataca ccattgccaa aaccgccgtt tctgccgctg ctcgtctgtt tgctgctcgc
     1021 ctttgtaatg aacaaattgg cgtttatgaa gtgcgccctg gtctcatcaa aactgatatg
     1081 actattccgg caacggctta ttacgacgaa ctgattgcca aaggtctggt gccgtgggga
     1141 cgttggggtt atccggctga tattgcttcc accgtccgcg cgatggcgga aggtaaactg
     1201 atttacacct gtggtcaggc ggtcgcaatc gacggcggct taagtatgcc gcgcttctga
     1261 ggctgaatca tgagagatct gttacaacgc ccggatttat tcagtatcaa taccgcgaca
     1321 ctgggctata aaacgccgct acccgccatt attgatgcct gcgccgcacg cggtattggc
     1381 gcaattgccc cctggcgcag ggagttgcag ggtgaagatc tccagcaaat cacccgccag
     1441 cttgccgcca gtaacatgag cgtttccggt ttgtgccgta gcacttacta caccgcgccg
     1501 acactggcgg aacgcaaact ggcgattgat gataaccgcc gggcgctgga cgacgcagcg
     1561 gtgcttaacg ccgcctgtta tatgcaggtg gttggcggtc tgccaacggg cactaaagat
     1621 ctatatgaag cgcgagaaca agtgaaacaa gggattcgcc agttgctgcc acattcgaaa
     1681 gatgtcggcg tgccaattgc gctggagcca ctgcacccga tgacagctgc cgatcgctct
     1741 tgcctgtgca cgttacgtca ggcgctggac tggtgtgatg aactcgatcc cgatggtgag
     1801 tttggcctcg gcgtggcggt ggatgtttat cacgtctggt gggatccgga tctcgccagt
     1861 cagatcctgc gtgccggaaa acgcatcctc gcgtttcatg tttccgactg gttaatgccg
     1921 accactgatc tggtcaatga ccgtggtatg ccgggagatg gcgtcatcaa tattccgtca
     1981 attcgtcggc tggttgaaaa cgccggattt aatggtgcta ttgaactgga aatattctcg
     2041 ccttactggt ggcagaaaga tattaacag
//
LOCUS       DAZPAB010000361         2049 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZPAB010000361 DAZPAB010000000
VERSION     DAZPAB010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.365"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(114..1568)
                     /gene="ospC2"
                     /locus_tag="ACHUPG_004217"
     CDS             complement(114..1568)
                     /gene="ospC2"
                     /locus_tag="ACHUPG_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HFR0863559.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
     gene            1972..>2049
                     /locus_tag="ACHUPG_004218"
     CDS             1972..>2049
                     /locus_tag="ACHUPG_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR0863560.1"
                     /translation="MSIRCPSCSATEGVVRNGKSTAGHQR"
BASE COUNT          639 a          359 c          333 g          718 t
ORIGIN      
        1 tcaccacatg caatgtgttg ttgcagggga acggtctgca ccatccccta tatcgatcaa
       61 tataacaaaa atgacaacgt cagattcagg gcacaaccta aatatatttt aggtcacctc
      121 ccaaatcgtt tgcctgatac cgctccattt tttaataaaa aatcaattgt ttttgaatct
      181 ttgcttttca ttgcattgtc aagcatagtg tccccactat ttgctttttg aaatcttttg
      241 tttacatcaa ccaatccatt actaataaaa tattctagca ctttatagtt ggcactatct
      301 tcactaagta aatactcaac atcatacagc tcacaataca tattgttgtt cattttctcc
      361 atctctgcga tatcttgttt tgtaaaatta aatttagata gtaaataaag tgccatatca
      421 gattttgcat tgcctagaga ataagcaaga gcttgatgag ccattttttt atcagtcacc
      481 ttattgttaa tttcttcata attggatgct ttaatggcat cctccaaaga tatatctctg
      541 agtcttatct ttttgaagtt atctgtgctt agcattcttg gtatatgata ctcaagctga
      601 aacacaaagt ttataattct atccagacta acaggatcaa tgtttttatt ataacaaaac
      661 tctcggaatc tttgatcttt actctttctt ataaaatcaa taagatgcaa cccaattcca
      721 agcctcatat ctttagtatt aaatattggc acatcagttt ttccctgatt accatgcaca
      781 tacctgctaa cttcatccac aacctcttta aagttatcta agaaaaatga ttggtgctca
      841 tggtaaaaaa caggtggaat agcattatca aagtggtcgg ttaatgtcat atatccatat
      901 ggagagtcat gatcaatgat atacgcatta gcaccaaaat ccacagtatg atgtgtttta
      961 tttagtggga gtgtttcttg atgattggaa atttcaaccc caaaaaaaac aaagtcgtgg
     1021 ttagctattt cttcaatatc ctcagagaat gtgtttttat atgtttgtat attctcagac
     1081 ttaattttat caaaggactt aatattaaga gtattatttg acaggttagc gttactttga
     1141 tgtctgaagt taaaatcaga attaataata atagagagca agtgacgttc atccgaagaa
     1201 agaggatgag ttttttgtat tatttcagaa agatagtgtc tggacacctt atcgaggtgc
     1261 tcaagtgacc gaaatgatga ccgtgctcca gatggcgctt caatagaaag atttaatgat
     1321 ttgaaactgg tcccttgaga gaacatttta ctataacttt gcgctgctat agacttcctt
     1381 aaaaagcttt tcacagtatc agcacagtga tctaaatgtt tttcacttat gccagaagag
     1441 gatgagtttg ttacgcatga tatgtttttt tgtaatgctt ttgtatcttt gttcggattt
     1501 atttttccat caacaagatc aatattgttc tgaacattaa tatgatttac tgcttcaggt
     1561 attttcataa aacatccccc attatgtgcg tttttatctg cagttattta gaataatatt
     1621 atctaactta atattttctt gtatgtatta taacgaacat attttaatat gttgtacgat
     1681 tttcactctg aaatcccctg agtttaatca cacttccttt actgaaagga attattgcaa
     1741 cagtattatt tgtttttgga tatatatctt gcgccttgtt atgtagttaa tgctcagaat
     1801 atgatagata aaatagagac gcaaaacagg gcatgctcat aacatgatga taacataaaa
     1861 aattagtaac aataaatgaa agaatcatgg gcgctcaaca aaaagagggt aatgctgcca
     1921 acttactgat ttagtgtatg atggtgattt taaggtgctt gcgtagcttc catttccatc
     1981 agatgtcctt cctgctccgc tactgaaggc gtggtgcgta acggcaaaag cactgccgga
     2041 catcagcgc
//
LOCUS       DAZPAB010000362         2044 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZPAB010000362 DAZPAB010000000
VERSION     DAZPAB010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.366"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(55..324)
                     /locus_tag="ACHUPG_004219"
     CDS             complement(55..324)
                     /locus_tag="ACHUPG_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0863561.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            381..1049
                     /locus_tag="ACHUPG_004220"
     CDS             381..1049
                     /locus_tag="ACHUPG_004220"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0863562.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1365..>2044
                     /locus_tag="ACHUPG_004221"
     CDS             1365..>2044
                     /locus_tag="ACHUPG_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0863563.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          614 a          447 c          381 g          602 t
ORIGIN      
        1 cagccaaccg cagcacgtcc tgcatacgtc gtgtctgcgg tttttctttt tcgcttacat
       61 tgtgtctggt tcttccggcc actcaatatc aggtgcagtt gatgtatcaa cacggttcag
      121 caacacccga tacttcttcc aggcttccag caacgagttt tcttcctccg ttgcgatctc
      181 cagatctaca gcatcctgaa gtggcgcaat atgctcactg aatccctgga tgtagaactg
      241 tgtggtgacg gtcttccagc cattcggctc ctgctgtatc gaagcatacc aggctatttc
      301 aatatcgcta tgctgcggca gcatttaacc ccttgtaatt catcgccata attgatttaa
      361 ttcacaaata aaactataat atggtgaaat taatgaaaaa aaacacagat gatggggcta
      421 aaatttacac accacttacc ctaaagcttt atgactggtg ggttttggga gtatcaaatc
      481 ggcttgcatg gggatgtcct acaaaggaac accttcttcc acactttctg gaacatttag
      541 gtaacaacca tctggatatt ggtgttggaa ctgggtttta ccttactcac gtacctgaga
      601 gtagtctgat atctttaatg gatttgaacg aagctagcct gaacgcggca tctacaaggg
      661 ctggggaatc aaaaattaaa cataaaatta gccatgatgt ttttgaacct tatcctgcgg
      721 cgttacatgg tcaatttgat tccatttcca tgttttacct tcttcactgc ctgcctggaa
      781 atatatctac aaaaagctgt gtaatacgca atgcggcgca ggccttaact gatgatggaa
      841 ctctatacgg agccacaatt cttggcgatg gagttgtgca caatagcttc ggtcaaaaac
      901 tgatgcgcat ttacaatcag aaaggcatct tttcaaacac aaaagattcc gaagaaggct
      961 taacacatat actctcagag catttcgaga atgttaaaac caaggttcaa ggtactgtag
     1021 taatgttttc cgcttcaggg aaaaaatagc atccaaccgc agcacgttct tgcttaagac
     1081 gtgctacggt ttcctttacg atttggggtt gcgactttac ctctattgat aatgcattcc
     1141 ggcagaacgt tcaaatatga acgtacgata tttaactaac cgaaaaacaa aatatagcac
     1201 aggcgagaca tttaccatct aagaaaatag tatcgttttt ttacagttaa atcaacatca
     1261 cttccttaaa atgaaaacaa taaatttcaa tcctgaaaca ttttatttca gcttattatt
     1321 ccccagtatc acattttatc aggatatcct tctgcgaggt tattatgctt cctgtaaata
     1381 atccccccct atccactgga aacgtctctt tttacagaac tacatcaatc gacaatgttc
     1441 acaataatta tctctccgaa tgggttgaat ggactaaaaa cagcatttcc ggagaaaaca
     1501 gggaaactgc ttttacccgg ctccaattat gtctggagaa cagtgaaaca tcgttggact
     1561 tatcttgttt aggtctcaga tctctaccac gattgcctga caatcttgat gaaattaatg
     1621 taagcaataa ccaactatca atgctccccg agctaccaag ggcattgaaa gagctgaatg
     1681 caagcagtaa tcaattatct gcacttcctg aattaccagt gtcgctggaa tatataaatg
     1741 tgagtgataa ccatttgttc gcacttcctg aattacctgc gtcactagaa tatattaatg
     1801 taagtgacaa tcacctgtct gtacttccga ggttaccaat gtcattggaa ttacttgatg
     1861 cagccagaaa tgctttggaa gtaataccag attttccaga aagagatgat catattataa
     1921 gaatattctg gcttaatcag aaccggatca cggcaattcc ggaaagcata cttggcctca
     1981 gttctgatag cgttgtcaat cttagagaaa atcaactatc tcccagaata atgcaaactt
     2041 tgtt
//
LOCUS       DAZPAB010000363         2041 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZPAB010000363 DAZPAB010000000
VERSION     DAZPAB010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.367"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(13..901)
                     /locus_tag="ACHUPG_004222"
                     /pseudo
     CDS             complement(13..901)
                     /locus_tag="ACHUPG_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          551 a          476 c          474 g          540 t
ORIGIN      
        1 gtattaagac tatcacttaa ataagtgata ctggttgtct ggagattcag ggggccagtc
       61 tagcagctgc tctacggtca tatttttaat tgcgctccgg tttacaagag cccacccctg
      121 tttttccaga taaaactgga aagtatccag ggtacagacc agtgcaccgt caggaacagt
      181 ttcggtgaat ctgatattgc cgtgttcgtc gaagtgcaca accagagtgc gaccgttacc
      241 cggaatcatc ttgtcagcgg acggggtatt attctggtgc agttctgcct ccatgcggtc
      301 gaacatcagc gatgtaggcc tctttgaatg cagcggcttt tttcccagtg aagcccatca
      361 ccaggaaaac gaagccgttt ttggtgattt ggtacattgg gcgtttttcg cctttggtgt
      421 cggtgtaggt gacgtcctca aaattgaggg cgttaaattc aggtgagcat tcagtatttg
      481 cgatggcacg taacacattg tcgtgtcgtt tgtggaagaa ctctgcaacc gcaacagacg
      541 tagtgacagc gcgaccgttt tcgatggtta cgtcagggtg agaaagggca gggatagtag
      601 ccatgatggc atcctcgagt gataagttaa ttaactcacc accgaggttt tccacgacca
      661 taagggtggt gagacgtaca ggggtggaaa taccggtcac tcgagaaccc ggccagtctt
      721 gcgactgccc tgcacatccc accataattt gaatggagct gtgcattacg cataaaaaaa
      781 ccgcttcagc gcggctatgc gctcgagtaa cgttcgggtt tccacgcccg gcccccgttt
      841 tatgaggtgc agatgcacta taattccacc cgttctggtt ttcaatagct acattcaaca
      901 ttttctctta cctttcatca ccgaagtgaa ctttgttgat gcggtgcctg gtgcctccag
      961 gtgacgttaa ccagttaaca attaacgccg gatacaggga aacccataac cacccgatac
     1021 gtgggaacta atcgcttttt aactgttccg cgtgcgctca gccgcattca ccgcatcaca
     1081 aaattcactt taaaaagggc ggacatcagc cagcaatgaa actgatgccg ccaaaggtac
     1141 caatcaacat ggagtgttgt ggcgtggttg tcacttaagc gtatggtcaa cctgacaacc
     1201 cggtgtcctc aacggggaag gaataactcc gccatactta ccgccgcgtc atttcgcgtt
     1261 gtgtgcctgc ttttaactac gtcaggcgag gtggttcctg ttattcccca acaacaagaa
     1321 atttgtataa tctgaatacc ccaacaataa gaagagtgag caagtgatgt attagcgatg
     1381 tcagctctca aagagactgc atcaatgtta aaattattca atgaagcaaa aactcaggct
     1441 gaggttgatc gcgctgtgtc tgaaataaac tctaagttgt caagcattca gcttgaatat
     1501 ttgtcatctc attgatgagc tgacaatctc taaaagcaat gaggctgcgc tcaaagcaaa
     1561 actcacggag atagaaaagt ttaagttcca aagtgaagac tgcgaacctc gctaattgat
     1621 tactggaacg attgtgtatg ccagacagtg caatactagc tctggcaaga ttaccatgta
     1681 tttttgtcca gaatgttttg cgaagagagc aatatctata ttgcaacctt tccctatcga
     1741 ggaaggcgat aaatatacga aaactcaatg cccctcctgt aaaaatgagt atctcatgga
     1801 ctacaatttc aataatgatt tttgagtcac ggtattcaga tttttaaaga acatgccgga
     1861 tactcacccg tgtccggcgc acgcactcca cctcacccgt ggagaactcc ttaattacca
     1921 accttggctt cgttggttag ccattaacgc tggtatgtaa tcattctggc aatgcttaat
     1981 ttgaagtggc acactgaatt tggccacctg aacagaggtg atatgctcac ctcagaacaa
     2041 c
//
LOCUS       DAZPAB010000364         2037 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZPAB010000364 DAZPAB010000000
VERSION     DAZPAB010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.368"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..346
                     /locus_tag="ACHUPG_004223"
     CDS             <1..346
                     /locus_tag="ACHUPG_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFR0863564.1"
                     /translation="EKWKYRFNFYDQHGFPGFWGATPEYKAAFKALKVRQRLTIQMNF
                     IAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVAAYVAVNTNKWFY
                     EKEVKGLNTWSL"
     gene            complement(387..1337)
                     /gene="corA"
                     /locus_tag="ACHUPG_004224"
     CDS             complement(387..1337)
                     /gene="corA"
                     /locus_tag="ACHUPG_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR0863565.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1489..1545)
                     /gene="ysgD"
                     /locus_tag="ACHUPG_004225"
     CDS             complement(1489..1545)
                     /gene="ysgD"
                     /locus_tag="ACHUPG_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR0863566.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            1707..>2037
                     /locus_tag="ACHUPG_004226"
     CDS             1707..>2037
                     /locus_tag="ACHUPG_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863567.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          545 a          491 c          493 g          508 t
ORIGIN      
        1 tgaaaaatgg aaataccgat ttaattttta tgatcaacat ggctttcctg gattctgggg
       61 agcaacacca gaatataaag cagcatttaa agcattaaaa gtaagacaac gattaactat
      121 tcaaatgaat ttcattgctt tcttctgttc ctggatctat ttatttgttc tgggcttatg
      181 gaaaaaagca attatagttc tattacttgg cattctatct ctatttgttg gtgcattaat
      241 tggcgttaac attctgggaa ttgccgtagc agcctatgtt gctgttaaca cgaacaaatg
      301 gttttatgag aaagaggtaa aagggttaaa tacctggagc ctttgaataa caggtggctt
      361 agccaggcta agccaccgcc ttctttttac aaccagttct tccgcttaaa gtacagatac
      421 ggtgccaggc ccgcgaggat cataaagata atcgcgccag ggtagccgaa actccacttc
      481 agttctggca taaactcaaa gttcatcccg tagctggaag caaccagcgt tggcggcagg
      541 aacacgacgg ataccaccga gaaaattttg atgatgcggt tctgctcgat gttgataaaa
      601 cccattgccg cctgcatcag gaagttcacc ttctggaaca gggattcgtt atgcggcagc
      661 agggattcga tatcgcgcag gatttcacgc gcctgctcca gttgcccacc cggtaaacgc
      721 gctttacgca ccaggaagtt gagagcgcgc tgggtatcca tcagacacag gcgaactttc
      781 cagccgatat cttccagttc cgccagcgtg gagagcgcct cgtcgtactc atcgccctga
      841 tgcccttcca taatcacccg gctcaactgc tccaggtcgc tatagatatt ttcaatttca
      901 tctgccaact gttcgatttt ggtttcgaac agatccagca gcaactcgta ggcgttaccg
      961 tctaccatcg actggctacg ggcacgcata cgatacagac gaaaagcggg cagttcacgc
     1021 tcacgcagag taaacagacg accatcacgg atggtaaatg ccacagtgga gttaccggcg
     1081 tgatcttccg catcttcaaa gaagaagaag gagtgaatat gcaggccgtc gtcgtcttca
     1141 aagaaacgtg ccgatgcttc gatgtcttcc agttccgggc gggttgccag gctctggcca
     1201 agttcagatt gtacgcgcag tcgctcgtcg tcgtccggtt cgacaagatc aatccatact
     1261 gcatttacaa ggggttgtga ctcttcgact tccagccggg tcagtcggtt attttccagt
     1321 tgaaatgcgc tcagcatgac cgggactccc aatgcttaaa atatcggaca gttcggtggg
     1381 cacacagaaa caaattgggt ttcagaccat taaacagcct gactcagcgc gacgggaaaa
     1441 attgaggtcg ctgacaaccg ctaaggctat cagcaaaaag ggatagcctt aggagttgat
     1501 cctggatgac aggatagtga gccagtatct actgggtgtg tccaaggcga atgtcctctt
     1561 agagtgatcg tgcgcgcatg ttacgccagc aaaattttgc cgtcaacacg caacagaaca
     1621 ccacagaaca atagttgaac ttcgcgcttt ttggtactcg tcaaggtcgc taacaggata
     1681 aaaaaatccc acatctagga cctataatgg cgcatcggct actcattggt aaaggaatga
     1741 tcaccttgaa tctcaaacgg atttttctca ccctcacctt acttcccttg tttgctgttg
     1801 ccgctgatga ttgcgcactc tcagatccga cgctgaccgt acaggcgtat accgttaatc
     1861 ctcaaacaga gcgggtgaaa atgtactggc aaaaagccaa tggcgaagcg tggggaacgt
     1921 tacatgctct gctggcggat attaatagtc agggtcaggt gcagatggcg atgaacggcg
     1981 gcatctatga tgaaagctat gcgccgctcg gtttgtacat cgaaaacggt cagcaga
//
LOCUS       DAZPAB010000365         2032 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZPAB010000365 DAZPAB010000000
VERSION     DAZPAB010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.369"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(79..155)
                     /locus_tag="ACHUPG_004227"
     tRNA            complement(79..155)
                     /locus_tag="ACHUPG_004227"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(119..121),aa:Arg,seq:tct)
     gene            complement(257..332)
                     /locus_tag="ACHUPG_004228"
     tRNA            complement(257..332)
                     /locus_tag="ACHUPG_004228"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(297..299),aa:Ile,seq:cat)
     gene            complement(477..1298)
                     /locus_tag="ACHUPG_004229"
     CDS             complement(477..1298)
                     /locus_tag="ACHUPG_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0863568.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(1313..1669)
                     /locus_tag="ACHUPG_004230"
     CDS             complement(1313..1669)
                     /locus_tag="ACHUPG_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0863569.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(1682..>2032)
                     /locus_tag="ACHUPG_004231"
     CDS             complement(1682..>2032)
                     /locus_tag="ACHUPG_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR0863570.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT          499 a          576 c          436 g          521 t
ORIGIN      
        1 ataatatttt caacaatatc ctgagccagg tatatttcag tataaggctc tgccggaagg
       61 aatctggaag aatgaatatg gcgcgctgta ctggattcga accagtgacc gattgcttag
      121 aaggcaattg ctctgtccgg ctgagctaac aacgctgaat accgataatg gaccgccatc
      181 ggggacccgc cccccgcacc aacaaccctg ttatcgtgtc gtctgctctt cctgataagc
      241 taatggcggt ttgtgatggt ggcccttgct ggatttgaac cagcgacctg gcgattatga
      301 gtcgctcgct ctcaccactg agctaaaggg ccgggagcag aataataacg gtccgtaatt
      361 aatttcgcaa taaaaaaacc cgctcagcgg cgggttgtag aaactcttct aacgccaggc
      421 ataaaacgcc catcattatg acgaatttac cacagattcc ggaaaaatca accttgttac
      481 ctagttacct tttttaactg ccgctcagcc catgcttctt caatatcaaa ccgggtcacc
      541 agcgcatcat aaaatttctt aactgttttt tcccatgacg cgcgtgttat ctggtttgtc
      601 acctcgcata tagcattaaa tgcctccgtt gatggtagtc tttcatagcc acgaccacca
      661 caacgctggc agtctctgat aacaggcata ccacgtttta ccgactcttc acgatgaatg
      721 gcgacaccac gcccacggca atccttacag gcggtggaaa cctcaccctt tccgccacac
      781 tccggacagg caacttttac cacctccctg acttttttcc attcttccca gtaaaacgga
      841 tacacacctt tcgtacactt tgcccatacc ggcggcttac catccggata ctggatcttg
      901 tttgtaaaaa ctatgctttc aataaatttt tccccatagc aacaagggca ctgcttttta
      961 ctcgctgcgc tgcgggcata atcctcaaaa gcgtacgaag ccataatgcg catcactacc
     1021 ggttttattt ctgccggaag ttttctcaac gccgccacac gatcgcaccg actgagtgca
     1081 taatctgcca gtaattctgt tgcccgcgcc ctgtcattca tactgatgcc cattttcccc
     1141 aggaacgcag aaaaacccat ctcagcccga ttctgtgtca tgccctgcgc ggccatcaca
     1201 tcagtgatac tcagcgcatc ttttgacgtt gaggccgatg catcggtcag gccaggggat
     1261 tttggggagt agtatttcgg taaatcttcc agtttcattt tttgacctgc tcttaatgca
     1321 ttatggggta aatcttcacc cccagacgtc caccagatac tggctgacca cgaacgatat
     1381 tgatttcatc aaactgctca tcgtccatta acactcccgc atgcgtcagc gcatccagcg
     1441 gtgctttcag gatattgtcc aggtcgcgac gacgcttatc cggtggctct gcaatcacct
     1501 ttatcgccag ccttccggac aggcttaatt tcagccgctg ctggcgaaca ataagcgcca
     1561 cagcccggcg ataacgcttt ccctcctccg agataaaata tgtgctgcca cggcgtcgcc
     1621 agtaagtgtt caccgtcggc gggtaaggta aaaccaaatc tatgagcatc agtcacctct
     1681 tttacccaag cacgccagtt gcaaaggcgt gatcaagaaa acgaaaaatt aaatcaacct
     1741 gagaaccatg cttttcttcg aacgccagag gatccgcatg aagctcgttg tgatgctccc
     1801 gacacagcgg tagcgtgaaa atatcgtgag atttggtccc cattccgccc tgaccatgac
     1861 caatcaggtg atggggatcg tcggctggct taccacaaca cgcacacggc tgtgtcttca
     1921 cccagcgttt gtatttctcg ttaacccagc ggcgacgttt aggtcgtttc atgaaagatt
     1981 ccggagactc aggatcaacg gcaatgctga ccaccgtctt ttcctgttgt gg
//
LOCUS       DAZPAB010000366         2031 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZPAB010000366 DAZPAB010000000
VERSION     DAZPAB010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.370"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..362)
                     /locus_tag="ACHUPG_004232"
     CDS             complement(<1..362)
                     /locus_tag="ACHUPG_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0863571.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACHUPG_004233"
     CDS             complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACHUPG_004233"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFR0863572.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          632 a          492 c          413 g          494 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt acgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca
     1741 ttaaaaacaa aaatttctac cgaggagaaa aataccctca aaaaccacct gaataattaa
     1801 ccattattgt taaaacaaca cgttgaataa tcacataaca aactccatta atcattgaaa
     1861 atgagcacag aaatagaaat taattatatt caattaatta aaatgtgtat tgcttgatgt
     1921 atttaaaaat attattttca tggtgagagt ggaggtaaag aagacacaat aataaaaagg
     1981 aatgtgacaa ataatggttc tggttgggcg ttaaatttaa ggtcaccaga a
//
LOCUS       DAZPAB010000367         2029 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZPAB010000367 DAZPAB010000000
VERSION     DAZPAB010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.371"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..1824)
                     /locus_tag="ACHUPG_004234"
     CDS             complement(<1..1824)
                     /locus_tag="ACHUPG_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFR0863573.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
     gene            complement(1932..>2029)
                     /locus_tag="ACHUPG_004235"
     CDS             complement(1932..>2029)
                     /locus_tag="ACHUPG_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0863574.1"
                     /translation="WNDEKPETIQPQSRPSSAITVSKTGYVFFDR"
BASE COUNT          485 a          579 c          430 g          535 t
ORIGIN      
        1 gcgcagttct tcacttgctg aggaaaaaac aagcgttcca tccttctcga catacccatc
       61 cgtttcaagc agaccacaca gcatgcctat acgcgttgat ttatttgcgg aaaaaaacac
      121 acgagggatg aatttgtttt ttgctgtgca ccccattagt ccataacctc gcagggtctc
      181 catgagtgga tttacctgac cacgcgccgt tgatatcagc cagtccttac catctccaga
      241 gaagttgtaa tcaggcagtt cagccttcat acgctcgatc atgtatggct cgacgtttga
      301 aaacttcacg ctcccattgc taagactacc atccccgaga agggagccga taacccatgc
      361 taatggctct gagtggtcgc caaagtcacc agttatttca ggaatcctaa cgccattttt
      421 gtgcctcttc gtttcactca accgcttcaa cccgaaggta tctatcacgc ggcgtttctc
      481 gcctttcgtg aatccacgac tagtgacctc ccataggtga tcgcctgcgc aatcaacata
      541 acgaccatct tcaaatgtga ctcggtaaac atctctaaca ccctgcggga atatcccagt
      601 gacagttgac ggattgccgt ctactgacgc cacctgatcg ccaattttta cattcccgtg
      661 agtagtccat gtcccatcag ctaaaagaat aggctcattc agtgggcatg cctttccgtg
      721 accgtgcaca ccattgacca gcgtcaactc tgcctcacgg aactggaatt tatctgccag
      781 agattcccac ggtggattaa acagatactg ctgcttgccg tagaaagcgt tgatagtgtc
      841 ctggtaaaac tctcgcgcac tgtagagttc ttcaggatcg aagtaggatg ccgtgccgat
      901 gtactgccag atttcatcct cggtaacacc gttcatcagg cattcgttga tgtctttgta
      961 cggcagagta acaagacggc aacgatgttc accgagtcgg cttacgattt cccttgcggc
     1021 ttcacgacca acatcatcaa cgtccatcga gatgaatatt tcctcaaacc tgtcgaggtt
     1081 gtgatactca aactcaatcc actgttgctt agcgcctttc ccgccaccaa acggcacgga
     1141 taacgccgag atgccgtatt gcgcatagct catacaatca atttcgcctt cgcaaagcac
     1201 aaccaccctc acgccagcgt ccagagcctg ccatccgaac aggcaaggtt cgcaatcacc
     1261 ttctgccata atgactttct tcccgtccgg gcgctcagtg ctgattcgct tgacctgcaa
     1321 caactcacca tcgcgtttgt acggaagcac cagagcatcc agttctcgtt ctccattcca
     1381 caccttgccg ctgacaacct cgtagcgctt tacgacttct ggcgatatgc cacgcgattg
     1441 caggtactca agatgggatt ctgttctggt aacgtagcgg gcgattttct tacgatcagg
     1501 tctggagaat ttcttctcac gtctggcatc gaaatggtga tcgtcatcct tgataccgag
     1561 aaatgctttc gcttcctgca tagcctgatg caggttaatt ccacgacatg ccatccacaa
     1621 atcaagcatg tcaccgccgt ctccctcagc gaaatcagcc cattttttct tgccgataag
     1681 gttgacctta aggctgtttc ccttgtcacc gttgacgtta ccggcaaccc actcatgccc
     1741 ctctatcttg ccgtttggca acaggtgcgg agccaccctg tcaacctgcg cccaaagcag
     1801 gtcgctaagt tcacttggcc tcatgattcc ctcagattga gatttttaaa ccagaaatcg
     1861 acaaacgaaa tacttaacca gccgtggtta taaccagcga ccagtagcga tttgattttt
     1921 gatttcatgg ttcacctgtc gaaaaacaca tagccagttt tcgatacggt gattgcggat
     1981 gatggtctgg attgtggttg aatggtttct ggcttctcgt cgttccagc
//
LOCUS       DAZPAB010000368         2023 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZPAB010000368 DAZPAB010000000
VERSION     DAZPAB010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.372"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..794
                     /locus_tag="ACHUPG_004236"
     CDS             <1..794
                     /locus_tag="ACHUPG_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shiE"
                     /protein_id="HFR0863575.1"
                     /translation="SETKAAYRCFSNDKVSANKIMTPHKENITERAQQFKRVLVLQDT
                     TELNYSGQKEKQGVGPKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKN
                     TRQEICNDLLHKKHVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFER
                     AAQTPGIKADWLVRMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQ
                     ARTLPDMLSSDAGPLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="ACHUPG_004237"
     CDS             complement(744..1937)
                     /locus_tag="ACHUPG_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0863576.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
BASE COUNT          581 a          503 c          491 g          448 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aat
//
LOCUS       DAZPAB010000369         1979 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZPAB010000369 DAZPAB010000000
VERSION     DAZPAB010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.373"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..179)
                     /gene="ospE2"
                     /locus_tag="ACHUPG_004238"
     CDS             complement(<1..179)
                     /gene="ospE2"
                     /locus_tag="ACHUPG_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HFR0863577.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTL"
     gene            complement(361..762)
                     /locus_tag="ACHUPG_004239"
                     /pseudo
     CDS             complement(361..762)
                     /locus_tag="ACHUPG_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1191..1979
                     /gene="virF"
                     /locus_tag="ACHUPG_004240"
     CDS             1191..1979
                     /gene="virF"
                     /locus_tag="ACHUPG_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HFR0863578.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          613 a          349 c          372 g          645 t
ORIGIN      
        1 ttcaacgttt tcgtccggtt ggcagcaggt atttgtaatt cgtatatagc tgctttttta
       61 ttgaaaacag tatatccatc tgtagtaaca gtagaagata ataatacagg atttgatact
      121 tcaaggatga tattttcttg tttttgtgga aggcaaggga atatagtttg tgtaagcata
      181 ttgcttcctt atgttatatt ttagtaaaga atttattttt tacattatag tcggctaaat
      241 gaagtttact gtaaaaaaac gatacttttt tcgtaagcga tgagatgtgg agccagaaaa
      301 atggctgcgt tacgtcattg aactgtcctt gacccacgat attcagacag tcgttgtatc
      361 ttaagttgac attacccgct gacgtcgata ttcccttggt gagagataac ccaacgcact
      421 atgcggatgg ttttcattgt aatgctcgat cgccactgca aggttatgca atgccgttct
      481 tggtttcggc atgaacgcga tgcagtcttc cttcatcgtt ttcatgaacc gttctgctat
      541 tccattactc tgagggctac tgactgccgt cgtgcagggc tccagattca actctctggc
      601 gaactgccgt gtttcatgag ccgtataagc cgaaccgtta tcggccactg gattggccgt
      661 tccggtcctt tatcgccata tcgctcctga catgacatcc tgcaccgttg ctttgtcata
      721 atctcccgtg cctgctgccc agtctatggc ttcacgatcg caatatggaa cgtaacgctg
      781 tcatcgggag aatcgtcaat gcggcattcg gttaacgctt accaatatca acaccaaccg
      841 gatttgtgga gcctccagtc tgaaggggct ttatgcgttc cgtataggat attatgatgc
      901 tggagttttg cgaagctcca ctcctttccg aacatttaca cagatattgc ctaagaaaag
      961 tgaaaccata atatggttat agtccctttc agtgcaaata cttagcttgt tgcacagaga
     1021 aatagaagct gcataagctc tttcttcaaa aaatgtaaat aaagttaaat ataggaaaaa
     1081 ttacttaatc tatcttaata acggaaagat tttgtataca atcactgtta cagaaatttc
     1141 ttagttactc tgtaaacact aaatatagtt tggtatattc tgttgaattt atgatggata
     1201 tgggacataa aaacaaaata gatataaagg ttcgcttgca taactatatt attttatatg
     1261 caaaaaggtg ttcaatgacg gttagctcag gcaatgaaac tttgactatc gatgaagggc
     1321 aaattgcttt tatagagcga aatatacaaa taaacgtctc cataaaaaaa tctgatagca
     1381 ttaatccatt tgagattata agccttgaca gaaatttatt attaagcatt attagaataa
     1441 tggaaccaat ttattcattt caacactcct attctgagga gaaaaggggg ttaaacaaaa
     1501 aaatattcct cctctctgag gaggaggttt ctatcgattt gttcaaatct ataaaagaga
     1561 tgcctttcgg caaaagaaag atctatagtt tagcttgcct tttatcagct gtttctgatg
     1621 aggaagcttt atatacttcg atatcgatag cttcttctct tagtttttct gatcagataa
     1681 ggaagattgt tgaaaaaaac atcgagaaga gatggcgtct ttctgatatt tcaaataact
     1741 tgaatttatc agaaatagct gttagaaaac gattggagag tgaaaaatta acatttcaac
     1801 aaatccttct tgatattcgc atgcatcatg cagcaaagct tttattgaat agtcaaagct
     1861 atattaatga tgtatcaaga cttatcggaa tatcaagccc atcttatttt ataaggaaat
     1921 ttaatgaata ttatggtata actccaaaga aattttactt atatcataaa aaattttaa
//
LOCUS       DAZPAB010000370         1977 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZPAB010000370 DAZPAB010000000
VERSION     DAZPAB010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.374"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            327..521
                     /locus_tag="ACHUPG_004241"
     CDS             327..521
                     /locus_tag="ACHUPG_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863579.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            529..1941
                     /gene="pykF"
                     /locus_tag="ACHUPG_004242"
     CDS             529..1941
                     /gene="pykF"
                     /locus_tag="ACHUPG_004242"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR0863580.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          555 a          481 c          447 g          494 t
ORIGIN      
        1 tcagcatata tcaatctaac caattaaaca aaaatcaaac aaaatcagac aaataacgcg
       61 ataaattatt ttaactgtag caattgagcg atgatatatt tatacaccgg atgaactttc
      121 acttatcctc acactgacaa cttcggcacc agacgttgcg caaacagcga agtttttgcg
      181 taaccttttc cctggaacgt taaatctttg ataacaattt attgtctaac aagttgtata
      241 ttttttgaaa cgctgttttt gttttccttt tggattaatt tcagcgtata atgcgcgcca
      301 attgactctt gaatggtttc agcactttgg actgtagaac tcaacgactc aaaaacagac
      361 actcacgttg ggctgagaca caagcacaca ttcctctgca cgctttttcg atgtcaccta
      421 tccttagagc gaggcaccac cactttcgta ataccggatt cgctttccgg cagtgcgccc
      481 agaaagcaag tttctcccat ccttctcaac ttaaagacta agactgtcat gaaaaagacc
      541 aaaattgttt gcaccatcgg accgaaaacc gaatctgaag agatgttagc taaaatgctg
      601 gacgctggca tgaacgttat gcgtctgaac ttctctcatg gtgactatgc agaacacggt
      661 cagcgcattc agaatctgcg caacgtgatg agcaaaactg gtaaaaccgc cgctatcctg
      721 cttgatacca aaggtccgga aatccgcacc atgaaactgg aaggcggtaa cgatgtttct
      781 ctgaaagctg gtcagacctt tactttcacc actgataaat ctgttatcgg caacagcgaa
      841 atggttgcgg taacgtatga aggtttcact actgacctgt ctgttggcaa caccgtactg
      901 gttgacgatg gtctgatcgg tatggaagtt accgccattg aaggtaacaa agttatctgt
      961 aaagtgctga acaacggtga cctgggcgaa aacaaaggtg tgaacctgcc tggcgtttcc
     1021 attgctctgc cagcactggc tgaaaaagac aaacaggacc tgatctttgg ttgcgaaaaa
     1081 ggcgtagact ttgttgctgc ttcctttatt cgtaagcgtt ctgacgttat cgaaatccgt
     1141 gagcacctga aagcgcacgg cggcgaaaac atccacatca tctccaaaat cgaaaaccag
     1201 gaaggcctca acaacttcga cgaaatcctc gaagcctctg acggcatcat ggttgcgcgt
     1261 ggcgacctgg gtgtagaaat cccggtagaa gaagttatct tcgcccagaa gatgatgatc
     1321 gaaaaatgta tccgtgcacg taaagtcgtt atcactgcga cccagatgct ggattccatg
     1381 atcaaaaacc cacgcccgac tcgcgcagaa gccggtgacg ttgcaaacgc catcctcgac
     1441 ggtactgacg cagtgatgct gtctggtgaa tccgcaaaag gtaaataccc gctggaagcg
     1501 gtttctatca tggcgaccat ctgcgaacgt accgaccgcg tgatgaacag ccgtctcgag
     1561 ttcaacaatg acaaccgtaa actgcgcatc accgaagcgg tatgccgtgg tgccgttgaa
     1621 actgctgaaa aactggatgc tccgctgatc gtggttgcta cccagggcgg taaatctgct
     1681 cgcgcagtac gtaaatactt cccggatgcc accatcctgg cactgaccac caacgaaaaa
     1741 acggctcatc agttggtact gagcaaaggc gttgtgccgc agctggttaa agagatcact
     1801 tctactgatg atttctaccg tctgggtaaa gaactggctc tgcagagcgg tctggcacac
     1861 aaaggtgacg tcgtagttat ggtttctggt gcactggtac cgagcggcac tactaacacc
     1921 gcatctgttc acgtcctgta atattgcttt tgtgaattaa tttgtatatc gaagcgc
//
LOCUS       DAZPAB010000371         1969 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZPAB010000371 DAZPAB010000000
VERSION     DAZPAB010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.375"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            240..509
                     /gene="yacA"
                     /locus_tag="ACHUPG_004243"
                     /pseudo
     CDS             240..509
                     /gene="yacA"
                     /locus_tag="ACHUPG_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
     gene            509..678
                     /gene="yacB"
                     /locus_tag="ACHUPG_004244"
                     /pseudo
     CDS             509..678
                     /gene="yacB"
                     /locus_tag="ACHUPG_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="ACHUPG_004245"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="ACHUPG_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HFR0863581.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1708..>1969
                     /locus_tag="ACHUPG_004246"
     CDS             1708..>1969
                     /locus_tag="ACHUPG_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0863582.1"
                     /translation="MTEALRLHFEEHLPRVVAGCRLGVPKSTACGMFVRFRNAGLSWP
                     LPAGMSEQELDALLYGSASTVPVVLTESTVMPKLPVVKKRPRR"
BASE COUNT          560 a          433 c          462 g          514 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
     1561 acaaggcgaa tcacgcacgg atttccagcc agagacacct gaaacgtaac cggctcattt
     1621 aaaccgtctg gtctgttacc tccggttcca caaaaataat gtccatcatt ttgaagtgga
     1681 cactatcgta tggaataccg gacctgaatc actgaagctt tacgccttca tttcgaagaa
     1741 catttaccac gggttgtggc cggatgtcgc ctgggcgtac caaaatcaac tgcctgtggt
     1801 atgttcgtgc gcttccgcaa cgctggcctt tcatggcctc tgcccgcagg tatgtcggag
     1861 caggaacttg atgcccttct ttacggaagt gcctccacag tacctgtcgt acttactgaa
     1921 agcaccgtta tgcccaaact cccggtggta aaaaaacgtc cccggcggc
//
LOCUS       DAZPAB010000372         1945 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZPAB010000372 DAZPAB010000000
VERSION     DAZPAB010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.376"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(16..998)
                     /gene="kdgT"
                     /locus_tag="ACHUPG_004247"
                     /pseudo
     CDS             complement(16..998)
                     /gene="kdgT"
                     /locus_tag="ACHUPG_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="ACHUPG_004248"
     CDS             complement(1258..1878)
                     /gene="sodA"
                     /locus_tag="ACHUPG_004248"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR0863583.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
BASE COUNT          487 a          516 c          469 g          473 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc cagcttcttc tttcgtaccg
      601 tactgctgca tgatggaagc gtaaagtccg ccgttggtca tatccatcgc cgccaccagc
      661 gccagcgttg aaagtccggc aaagaatcca acttcaacac catgttccgg aataatgcgc
      721 gaggcaattg ccgcaaccac ccacgcgacg gcaattttag ttaccaccag cgtaccggat
      781 ttacgcagta ccgttcccgt cgcgcttaat tttattgacg cccccatgca aaaaaaccac
      841 accgccagaa tgggcaccgt accggtaatc atcccgttgg tgaatgatcc aaaatatttc
      901 cccgcccccg gcgagaaggt gtggcacagt gcgccaagga atagcgggac gagcatcatc
      961 ccccccggga ttttctcaat cgagcgtttt atctgcattt ccatcacctg tcggcatatt
     1021 tttaagggct gatgcgtaat tcctcaatgg agcatcagtg ttgatggaaa cgaagataga
     1081 cattcattta atcaataaaa gtgatcaaaa tcacatcaat tgaaacgctg ttttattttt
     1141 ataggaccat tgtttttatt ttggaattga tcacaaaaaa accaccgccg ttggcgatgg
     1201 ttcatttttt aagctgatat gcggcctata cgcctcattg cagcaggcgg caaataatta
     1261 ttttttcgcc gcaaaacgtg ccgctgcttc gtcccagttc accacgttcc agaactcttt
     1321 gatgtagtcc ggacggcggt tctggaattt caggtagtaa gcatgttccc acacatccag
     1381 gcccagaatc gggaagccgg aagcgccaga aatagcttca cccatcagcg gagaatcctg
     1441 gttagcagta gaaaccaccg ccagtttatc gcctttcagc accagccatg cccagccgga
     1501 accaaagcgg gaagctgccg ctttttcaaa ttctgctttg aagttatcaa cggagccgaa
     1561 gtcacgttcg atagccgctt tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt
     1621 ccagaacagg ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc
     1681 tgctggcagc tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg caaattctgg
     1741 caggctttcc agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg
     1801 gatttccatg gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat acggcaggga
     1861 tggcagggta tagctcatat tcatctccag tattgtcggg cggccgattg ttaatgccgc
     1921 gtaagcagtt ggttcattat agtta
//
LOCUS       DAZPAB010000373         1913 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZPAB010000373 DAZPAB010000000
VERSION     DAZPAB010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.377"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(53..1702)
                     /locus_tag="ACHUPG_004249"
     CDS             complement(53..1702)
                     /locus_tag="ACHUPG_004249"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR0863584.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          452 a          506 c          491 g          464 t
ORIGIN      
        1 gaagaggata attttacagc cagcaggaag ttagcggctg gctgtaaaaa tattatggtt
       61 cgccgtacaa accaattaaa cggcgtacca caccgttagt ccagccaaac ccatcctgca
      121 acggatactc gccaccgccg ccttcgcggg gaacaccatc ggcaatatgg tatttctcga
      181 tcattttgtg ctgttccaga tagaactgat tcaccgtttt cagccagctg cgcgcgattt
      241 catcacccag aaggtcatcg ccatacattt taaatccctg aattgccatc cattgtaacg
      301 gtgcccagcc gttgggttta tcccactgtt caccggtttc gtactcgctt gccaaaatcc
      361 cgccaggtgt cagtaaccgg ctgcgtacgg cgtttgccag acgatcggcc tgttcatggt
      421 tcgccatgcc gacatagagc ggcacaatgg cggcagcgga aaacagcgcc agttgttcgc
      481 gtcgccagtc gtaatcgcgg tagatgccgt tttcatcatc ccagaggtaa cggtttaccg
      541 catcgcgacg ggcactggcc ttctggcgga acagcgcttc tgtctctttc tcgcctttca
      601 gcgccgagat gttggcgatg gcgctctcca gtttaaacag gaaggcattc agatcgatgg
      661 ggatgaactg ggtggtacga atgctcgcca gacgaccagt atcacgcagc caacgggaag
      721 agtaatccca accggaggcc gctcccgcgc gtaaatcgcg gtacacctcg ttgggcgggc
      781 gaccagaatg tttcgcggtt tcaacgtcct caagccagga ttcgtcacgc ggcgtgtcgc
      841 gatcgtccca gtaacggttg agcagcgatc cgtccggcat ccgcacaaca tggcgatagg
      901 cctgattagg gatcaacgat tctgcaccgt ccatccagaa ggcatattcc attttaaggt
      961 ggtcgagata gcggcgcgca ccgcgtacac catcttcttc aaacaactcc accatcagcg
     1021 caaaaaccgg tggttgcgag cggctcaaat aataggtgcg gttgccgttg gggatgtgac
     1081 cgtagttttc gatcatccag gcgaagttat cggccatgca tttcagcaaa tcttcccgac
     1141 cactttccgc cagtcccagc atggtgaaat aggaatccca atagtaggtt tcgctaaaac
     1201 ggccgcccgg gacaatatat gactgcggta gcgccagcag agaagaccac ggaatgtgat
     1261 cctgtggttc gcgggttagc accggccaca gctggtcgat atgctctttc agggaatttt
     1321 gcgggtccga tacatactcg ctggagtaga cctccggcag ccagaagtga ttttcaacaa
     1381 acttgcgcaa gtcaaaatca cgatgacggc gcactttacg gtagcggatt aaaatatcca
     1441 gcgggtccat tttgggtgcg cagtcgggaa aggttttact gtcggggaaa attttcgccg
     1501 actgaacatg ctcgaacagt tcgagatagc gatcggcagg cgtcagcgca tcagaggcgg
     1561 gcagcccttc aatcatctcg ggttccggtt ctgcctcgat catctcatcc agttttaatt
     1621 catacgggtc cagttcatag cagagatcga cctcgatcat cagttcgtct ggattagggt
     1681 tttgaatttt ctgattgagc ataaggatcc aacctccgaa attcgtcgct aattatcggg
     1741 cttttcttta agggtagtca ttcgctccgc ctggtgggcg acaaagcgtg cggtggatca
     1801 cgtttattaa cttgtcaaaa aatataacgc actaatatca atggaattta taaaaatacg
     1861 gatttggtgt tgtttttcat tttcagaaca ttcaatcctg ctctcgccgc aat
//
LOCUS       DAZPAB010000374         1902 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZPAB010000374 DAZPAB010000000
VERSION     DAZPAB010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.378"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..203
                     /locus_tag="ACHUPG_004250"
     CDS             <1..203
                     /locus_tag="ACHUPG_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863585.1"
                     /translation="GASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAI
                     YLLAGCLISAMTALLMKDNQRS"
     gene            305..1759
                     /gene="amn"
                     /locus_tag="ACHUPG_004251"
     CDS             305..1759
                     /gene="amn"
                     /locus_tag="ACHUPG_004251"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR0863586.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          466 a          472 c          445 g          519 t
ORIGIN      
        1 ttggtgccag ttatcgctac agtggtgctg gagtcggtta tcaggttgcc agtgtggttg
       61 gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc gggaactggc
      121 atagcatcgc catttatttg ctggctggat gcctgatttc tgcaatgacc gctttattga
      181 tgaaagacaa tcaacgctcg tgatagcctg gcgaagatca tccgatcttc gccttatact
      241 tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac
      301 acacatgaat aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc
      361 gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaaatata ttacaattgg
      421 cgaattacct gatgaaaacg cccgtaaaca aggtcttttt gtctatccat cactgaccgt
      481 aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc gttttactca
      541 cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt atctgaatga
      601 gcaactaacg ttgctttatc aggattatgg tgcgcatatc tctgtgcaac cctcgcaaca
      661 tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc gctcaatgag
      721 cgctgattta actcgctact tcccgacaac agaactggcg caaatcggcg atgaaactgc
      781 agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg cgcgccgcgt
      841 cgatttttcc ctcgcacggt tgcgccatta taccggtacg ccagtagaac attttcaacc
      901 gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag
      961 ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg ggaactggat
     1021 cactgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca
     1081 gatgccagca tggcatttaa ttaccgctga tggtcagggt attactctgg tgaatattgg
     1141 cgtgggaccg tccaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatat
     1201 ctggttgatg attggtcact gtggcggatt acgtgaaagc caggccattg gagattatgt
     1261 ccttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc cgcccgatat
     1321 acctattccg agcattgctg aagtgcaacg tgcgctttat gacgctacca agttagtcag
     1381 cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca ggtactgtgg taaccacaga
     1441 tgacaggaac tgggagttac gttactcagc ttctgcactt cgttttaact taagccgggc
     1501 cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc
     1561 atacgggaca ctactgtgtg tttcagataa accgttacat ggtgagatta agcttcccgg
     1621 ccaggctaac cgtttttatg aaggcgctat ttccgaacac ctgcaaattg gcattcgggc
     1681 gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac gaacctttaa
     1741 tgagccgccg ttccgataat aagttatgaa ccttcttatc agaaatagat gtgaaggatt
     1801 aagtaagaca tctggcaaat agcctgcaat aaacgcgggc tgcttgcttc cttgggcaga
     1861 tgcgagtttt attatcgtct taatgatttc cacatattaa aa
//
LOCUS       DAZPAB010000375         1846 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZPAB010000375 DAZPAB010000000
VERSION     DAZPAB010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.379"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..254)
                     /gene="ydfV"
                     /locus_tag="ACHUPG_004252"
     CDS             complement(<1..254)
                     /gene="ydfV"
                     /locus_tag="ACHUPG_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HFR0863587.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFS"
     gene            279..518
                     /gene="relB"
                     /locus_tag="ACHUPG_004253"
     CDS             279..518
                     /gene="relB"
                     /locus_tag="ACHUPG_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HFR0863588.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            518..805
                     /gene="relE"
                     /locus_tag="ACHUPG_004254"
     CDS             518..805
                     /gene="relE"
                     /locus_tag="ACHUPG_004254"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFR0863589.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            877..1032
                     /gene="hokD"
                     /locus_tag="ACHUPG_004255"
     CDS             877..1032
                     /gene="hokD"
                     /locus_tag="ACHUPG_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR0863590.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1250..1501
                     /gene="rem"
                     /locus_tag="ACHUPG_004256"
     CDS             1250..1501
                     /gene="rem"
                     /locus_tag="ACHUPG_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFR0863591.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            1568..1846
                     /locus_tag="ACHUPG_004257"
                     /pseudo
     CDS             1568..1846
                     /locus_tag="ACHUPG_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          506 a          409 c          466 g          465 t
ORIGIN      
        1 atgctaaagc aaaaaataga tgaataagtt gagttgtgca tatgtagcct gaccgtcaca
       61 aagtatatgg tgtctgtacc agtaagatga tggccggact ctttaaaaaa gagctgacct
      121 gcacaataca ggatggactt agcaatggct gctcctggca caaagcggac agtgatcacc
      181 gttcttacga ctactttctg acttccttcg tgacttgccc taagcatgtt gtagtgcgat
      241 acttgtaatg acatttgtaa ttacaagagg tgtaagacat gggtagcatt aacctgcgta
      301 ttgacgatga acttaaagcg cgttcttacg ccgcgcttga aaaaatgggt gtaactcctt
      361 ctgaagcgct tcgtctcatg ctcgagtata tcgctgacaa tgaacgcttg ccgttcaaac
      421 agacactcct gagtgatgaa gatgctgaac ttgtggagat agtgaaagaa cggcttcgta
      481 atcctaagcc agtacgtgtg acgctggatg aactctgatg gcgtattttc tggattttga
      541 cgagcgggca ctaaaggaat ggcgaaagct gggctcgacg gtacgtgaac agttgaaaaa
      601 gaagctggtt gaagtacttg agtcaccccg gattgaagca aacaagctcc gtggtatgcc
      661 tgattgttac aagattaagc tccggtcttc aggctatcgc cttgtatacc aggttataga
      721 cgagaaagtt gtcgttttcg tgatttctgt tgggaaaaga gaacgctcgg aagtatatag
      781 cgaggcggtc aaacgcattc tctgaaccaa agcatgacat ctctgtttcg caccgaaggt
      841 gacacttctg ctttgcgttg acaggagaag caggctatga agcagcaaaa ggcgatgtta
      901 atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac gaggaaagac
      961 ctctgcgagg tacgaatccg aaccggccag acggaggtcg ctgtcttcac agcttacgaa
     1021 cctgaggagt aagagacccg gcgagtgaga aatccctcgc cacctctgat gtggcaggca
     1081 tcctcaacgc acccgcactt aacccgcttc ggcggttttt tgtttttatt ttcaacgcat
     1141 ttgaagttct ggatggtgcc ggaatagaat caaaaatact taagtagcgc gcagggataa
     1201 aagggatgga ccccgaacag gggagtgcta cttatctgga aggattctga tgatgaacat
     1261 cgaagaactg cgtaaaattt tttgtgaaga tggcctctat gctgtgtgcg ttgaaaatgg
     1321 aaatattgtt agtcattacc gcattgtgtg tttgcaaaaa aatggggctg cgttaattaa
     1381 ttttgtggat gcccgagtga cggacggatt tatcttgcgc gacggtgagt ttgtcacttc
     1441 attacaggca ttgaaagaga tcggaataaa agctggcttt tctgcttttt cagaagaata
     1501 aactcatcta caatcttgcg cggggctgaa ctcccgctga gtaacaccgt gccaccggag
     1561 aaaaccgatg gcacgcaacg caaaatatta caatcatgat aattcgaccg ttcttgccca
     1621 cacgcacgag cggtattctc acgcatttaa gtcagactgg taccagcatc cctcatgcac
     1681 tgaagaacag gccgaatggc ttattcagtg ttaccgcagg cgcggatgcg aggttaaaaa
     1741 agcccttagc ctcgactacc gtcactagat aatctccgtc aggctccctt actccgaaca
     1801 gccaccgcgt ccgtcccgca cattccagca acggatctgg aggtaa
//
LOCUS       DAZPAB010000376         1836 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZPAB010000376 DAZPAB010000000
VERSION     DAZPAB010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.380"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(1..344)
                     /locus_tag="ACHUPG_004258"
                     /pseudo
     CDS             complement(1..344)
                     /locus_tag="ACHUPG_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(344..1264)
                     /locus_tag="ACHUPG_004259"
     CDS             complement(344..1264)
                     /locus_tag="ACHUPG_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0863592.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1402..>1836
                     /gene="pgrR"
                     /locus_tag="ACHUPG_004260"
     CDS             1402..>1836
                     /gene="pgrR"
                     /locus_tag="ACHUPG_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR0863593.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGV"
BASE COUNT          459 a          447 c          424 g          506 t
ORIGIN      
        1 cacacaccca gcacgccaat gcgttcagca tcaacatatg gcagagttac tgcataatcg
       61 ataacacggc taatatcctc tacgcgctgt gtcggatctt ctatccaacg aggctaccca
      121 ccagattctc cctggaaact ggcgtcataa gcgagaacga catagccttt ttcagccaat
      181 gccttgccat agatattgcc agacgtctgc tccttacaac tgccaaaagg atggacgctg
      241 ataatcgccg gatactgttt tgactcatca aaatttggtg gaaactgaat attggcagca
      301 atatgccagg ccatatcact attgcggatt tgaatatgtt tcattacaga gtttcctcaa
      361 agaacggagc taatactgaa attgcttcag ccacataggc tttgccgtca taaagatcca
      421 tatggtttgc gccttcaaca atgtgatagc gtttatcctg gcttgatgcc cgatcgtaca
      481 ggtcgtcact catccatttg ctccctgcct ggctgcccgc cacaatctgc atcggctgag
      541 tcaggtacac ttccgccata tggtaagcat cataggtaat aatctggtta aggctgcgca
      601 aagtagcgta acccggtgct gttggatact gcgcacgagg tgtgtggtaa tactcccagg
      661 cctgacgcag ttcttcgttc ggcgcatcgg cctccttcat tggagccagt ggcataatgg
      721 cgtattcttc gctgctaata tcgctggttc gggcgtttga gccagcttca atgtgtggca
      781 gagcatcaat agatttcaca ttgttttccc aaccattgcg gaacatcgaa ccaatattga
      841 ccgcactaac ggtaccgatg gccttgatgc ggcgatcctg aattgcagca ttggctgtat
      901 atcctgcacc ggcacaaatt cccatcgcac caattcgggt attgtcgaca taagaaagcg
      961 ttgtcaggta atcaatcacg gcactgacgt cttcagtacg aatgtatggg ttttctaact
     1021 gacgcggctc gccgccactt tcaccttgat aagatgcgtc ataagcaata gtgacaaatc
     1081 ccttttccgc cagcttttcg gcataggttc cggccgtttg ctctttaacg cccccacctg
     1141 ggtgagatac cacaattgcc ggatactgac gggtttcatc aaattttgga gggaaataga
     1201 tcactgcaga caaagagatg gttggattat tgctgttagt gaagctgact ttattattca
     1261 tcattcagtt cctcatggag ttgtcggttc gttttgacgg ttggtgatat cactatagat
     1321 attgatcatt aagttgatta gaccctaaat catgattaga cctatcattt aaatgattaa
     1381 tagattgtat ggagatactg aatgaagcgt gaagaaatcg ctgatctgat ggcgtttgtc
     1441 gtcgttgcag aggagcgtag cttcactcgc gcagcagccc gcctgagcat ggtgcagtca
     1501 gctttaagcc agatagtgcg ccgtatagaa gaacgattgg gattgcggct tctgacgcga
     1561 accacgcgca gcgttgtacc cactgaagcg ggcgagcatc ttttgtctgt tcttggccct
     1621 atattgcatg acatagattc tgccctgaca tccctgagcg atctgcagaa ccgcccatcc
     1681 gggacaatac gtattactac tgtagaacat gcagcaaaaa cgatattgtt accagccatg
     1741 cgcacattcc tgaaatcgca tcctgaaatt gatattcagc ttaccattga ttatggtttg
     1801 accgatgtcg tttctgaacg ttttgatgca ggcgtc
//
LOCUS       DAZPAB010000377         1833 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZPAB010000377 DAZPAB010000000
VERSION     DAZPAB010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.381"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..181)
                     /locus_tag="ACHUPG_004261"
     CDS             complement(<1..181)
                     /locus_tag="ACHUPG_004261"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863594.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
     gene            complement(322..876)
                     /locus_tag="ACHUPG_004262"
     CDS             complement(322..876)
                     /locus_tag="ACHUPG_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR0863595.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(873..1163)
                     /locus_tag="ACHUPG_004263"
     CDS             complement(873..1163)
                     /locus_tag="ACHUPG_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR0863596.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1163..1762)
                     /locus_tag="ACHUPG_004264"
     CDS             complement(1163..1762)
                     /locus_tag="ACHUPG_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR0863597.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          445 a          496 c          422 g          470 t
ORIGIN      
        1 ggttatgggt gatcggggag agagtttcaa cagagatttg agtggtcata acgataactc
       61 cgtacatttg gaaattatcg ccaccgacga cgccaatcga actggtggcg aactgtgcag
      121 ggttggcgta accgggtacg gaaaccggcg agcctttcgg ctcccccaca cagcccgcca
      181 taaatcgcga atgtgactgt gcaaacgata tgaaaaaaga cgcgggcgcg tctcatatcg
      241 ctccgtaaac atccgggacg ccaatcccga cgccagattt tgctggcgcg tgaggaatat
      301 agccccggat aacagattga gtcaacaaac ggtttttaga tccccggaag agaatgcatc
      361 acgcatcggt agatagagca tatattcagc aagatttagc catacatcta cccgactgca
      421 gcacgtccgg aaacaccact cagggtgtgc gtcattcagc aattcagcca ttttacgctt
      481 agtcatcccc cgcccttcat accgttgccg gaggacacta atcaatccag gatgctctgc
      541 cagcacctca cttatgactc tatcaataca taacgcctct gcatcagtac aatgcgccag
      601 ccaggtcttt tgcttgccat tgatcatctc tcgcaaaaac gcttccagct caggtttctc
      661 tattcccgct tttttcatcc tgcgcagggc ttcattgacg gctgttttcg tcaatttttt
      721 ggatgccagc aactggttaa acatatttcc cgtcttaccg ccgccaatat acgaccagcg
      781 cccccacatg cgcagttttc cctgaatcca gacactttcc agcgtggtga gacgaaggtg
      841 ttccccgctt ttgcctgtat ttgttgggta aatcataaat aaccttcctt tctccagatt
      901 tcttgtgtgc gaaaaacacc ttctgcatgc atcaggcgta attcttcttt ggtgtaatcg
      961 ctggttttta cccgcccgtc gattaaatcg tggcatgagc tacaggcaat cgctgcctgc
     1021 atatcgtgtg gttttgtcgc tgttccgcac gtccccgcca gcctgtaatg cgccagcaca
     1081 gaagtttcgg gattgtgatt gcagtagcca ggaattctga cggtgcacat ctgcccccgc
     1141 gccgctttac gtaaatccac cattacgcaa actccagtaa ctgcgcggcc acattttcga
     1201 cttcctccgg agaggaaaat ttacggaaca ggatccagtt ccacagcaca ttcagtacag
     1261 atttataaac ctgctgaaac tcggtttcgt ccatgttcgc aaatgcgata gattttgccc
     1321 tgcgcccacg actaccatca ggataaatat gctcggtgta aaatccggcc tgaatggtta
     1381 cccactcgcg gaaagcgtca aacgacttta gcaatgccgt atcccgggtt ctgcatgtcg
     1441 caactgtatt aaggtattgc tctgcggcat cactcagggc tggagtgtgt tcccgaccta
     1501 ctgattcgca caggtaatca acgaagcctg ataccagttt tcgttcgcga ggcgtgatcg
     1561 ccccaccgac cggagtccag taatcaaatc ccagttgcag gagtttgaaa aaacgcttgt
     1621 ggaatgcgta gttacgcaca cgcttaaagt ctgcgtgtat ccactcacct attttgattt
     1681 gatgcaaaaa atcgcaactc tccggcgtcg ccgggagaag taatccggaa gaggtttgtt
     1741 tgaccagttg tatatgcgcc atcgtagttc tccgctggcg cagtagaatg ggtgttcagc
     1801 ccgttatgta gtataccaga attaatgcca ata
//
LOCUS       DAZPAB010000378         1809 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZPAB010000378 DAZPAB010000000
VERSION     DAZPAB010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.382"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            173..1198
                     /locus_tag="ACHUPG_004265"
     CDS             173..1198
                     /locus_tag="ACHUPG_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0863598.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="ACHUPG_004266"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="ACHUPG_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          421 c          462 g          505 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctggct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtgag
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgcc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DAZPAB010000379         1808 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZPAB010000379 DAZPAB010000000
VERSION     DAZPAB010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.383"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1193..1795
                     /gene="fimB"
                     /locus_tag="ACHUPG_004267"
                     /pseudo
     CDS             1193..1795
                     /gene="fimB"
                     /locus_tag="ACHUPG_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
BASE COUNT          548 a          322 c          378 g          560 t
ORIGIN      
        1 tcaacccatt ttacccagag tcataaaata gagtgtctgt tgatctcatt ttcatattcc
       61 tttcagactg aagtgcattt tcatcacccg atgtgaaaga actgaagtga tgtttgccat
      121 agattacgag atgcccagtc tcattgacta tcgtggtgca tctttatacc tgttatacca
      181 gatcaaaaat cacgcaatcc atacaacaaa accagatttg caattcgcgt cacaaaatat
      241 gtcgatcttt ttctaagaag aagatgccat gtgaagccag acgaacactt gcggtggtct
      301 tcaaaaacta aagattttct tttaactata cgttttataa ataatttatt tagaatctaa
      361 acaaagaaag ctttgcaagc taactcagtg agagtggtga aaataagggt ttactcgcca
      421 tcaggctgag cataattctc atcatgaaat atgtttcttg gtttgtggct tgtaactggt
      481 cactcctgat gtcgatctgg agaggcttgt tgatgttggt gttttcagga tgatgtttca
      541 cttagtttgt ttgccgtatc gcccggcgaa tagctgtgat tgaggaaggc ttaagtcgta
      601 gtgaccaaag ctatatttac caacggatgt agatgaaaaa gcgtcgcctg cgttccccca
      661 tatctccagg ataaaaagga atgtaacaat ctcatggcct aagctgacga accaggcagg
      721 aataatcgct agggatctaa gaattagcat gataatagcc actaagaaat tactgcgttc
      781 catgaaatag ccattttgtg gcaaatgcag ttaactaata atgtcatatt tgagacggct
      841 agtttaacaa atattaaatt ttgctgaatt ttttatgttg attttacttg ttacagaaca
      901 tatcacatga tatatagata agattagttg cattaatgat gagggttatt attagattcg
      961 tatcagattg ataaatatat aaaggtacat agcatgcaag agcatggcgt ttgtatggca
     1021 aagttattat aagtaactat cgctactcca tttaagttcg ctcagaagaa ctggtccact
     1081 tacgtaagtt attaagcaaa cgttcgcttt tataaacata atcaggataa aaatgttgga
     1141 ttattgctaa cccagcacag ctagtgcgcg tctgtaatta taagggaaaa cgatgaggaa
     1201 taaggctgat aacaaaaaaa ggaacttcct tacccatagt gaaatcgagt cactccttaa
     1261 agcagcaaat accgggcctc atgcaacacg taattattgt ctgattttgc tttgttttat
     1321 tcatggtttc cgggcgagtg aaatttgtcg attgaggatt tcggatattg atcttaaagc
     1381 aaagtgtata tatatccatc gattaaaaaa aggcttttca acaacgcacc cgctattgaa
     1441 caaagaagtt caggctttaa aaaactggtt gagtatccgt acttcttatc cgcatgctga
     1501 gagcgagtgg gtatttttat cacgtaaggg gaatccgctt tctcggcaac agttttacca
     1561 tattatctcg acttctggtg gtaatgccgg gttgtcactg gagattcatc cgcacatgtt
     1621 acgctattcg tgtggttttg ctttggcgaa tatgggaata gatacgcgac ttatctagga
     1681 ttatcttggg catcgcaata ttcgtcatac tgtctggtat accgccagca atgcagggcg
     1741 tttttacggc atctgggata gagccagagg acgacagcgt catgctgttt tatagagata
     1801 gttgagat
//
LOCUS       DAZPAB010000380         1791 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZPAB010000380 DAZPAB010000000
VERSION     DAZPAB010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.384"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            412..816
                     /gene="stpA"
                     /locus_tag="ACHUPG_004268"
     CDS             412..816
                     /gene="stpA"
                     /locus_tag="ACHUPG_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR0863599.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(863..1387)
                     /gene="ygaP"
                     /locus_tag="ACHUPG_004269"
     CDS             complement(863..1387)
                     /gene="ygaP"
                     /locus_tag="ACHUPG_004269"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR0863600.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1397..1696)
                     /locus_tag="ACHUPG_004270"
     CDS             complement(1397..1696)
                     /locus_tag="ACHUPG_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR0863601.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          499 a          392 c          417 g          483 t
ORIGIN      
        1 atgaaagttt gggggggagg taacgctata agcgtagaga attcggtgtt tgtcatgacc
       61 gggtcattta cagcattttt gatgccagaa aatgtacatt aacagcagaa agcaccagtg
      121 aggggtgttt tcttttattc tgttatattg catttttatt cacttcggtt ttgtcaggct
      181 tgcggagtta gcgagcagag agcgccactc tgttcaccaa taacctaaat tactcatcaa
      241 aatggctaat atataatctt gaaattattc tgagagtttc agaaaataac agctgaaata
      301 atctcgcgca ggactgtaaa tagattaaat tttgtggaaa tataataagt gatcgcttac
      361 actacgcgac gaaatacttt tttgttttgg cgttaaaagg ttttctttat tatgtccgta
      421 atgttacaaa gtttaaataa cattcgcacc ctccgtgcga tggctcgcga attctccatt
      481 gacgttcttg aagaaatgct cgaaaaattc agggttgtca ctaaagaaag acgtgaagaa
      541 gaagaacagc agcagcgtga actggctgag cgtcaggaaa aaattagcac ctggctggag
      601 ctgatgaaag ctgacggaat taacccggaa gagttattgg gtaatagctc tgctgctgca
      661 ccacgtgctg gtaaaaaacg ccagccgcgt ccggcgaaat ataaattcac tgatgttaac
      721 ggtgaaacta aaacctggac cggtcagggc cgtacaccga agccaattgc tcaggcgctg
      781 gcagaaggta aatctctcga cgatttcctg atctaattac tgaggccgga tgtcgctgct
      841 agggcgcgtc cggcgtcgct tctcaagctc gttggttcca cggcatctta tctaacaacc
      901 ttgccattcc acaaaaaccg ctgattcctg caaacagtaa cccggcacca acaaagccac
      961 ttaataagaa gaaaccgcta tttacggtat aacccagtac aacgccgttt aatattaaac
     1021 cacccgcagc gatctgcacc tggcgcatta acggcaaggg ttgagattta tttaccgcta
     1081 ctggcaatcc agctcttttc cagccatcaa tcccatcttc gagtaaaaat atttctgcgg
     1141 gggcggcaat cgctgctaat ttatcggcgt tattactggt gcgtttacct gcctggcagt
     1201 ggaatataat ttgctcgcga cgtaatttag gtggaagacc tgactgttcc agcacggata
     1261 atggagccag atctgcttca ggaatatgtt cacgaagata ttcatcagcg tcacgaatat
     1321 cgattaactt tgcgccgcgt gcgattaatt cttgtgcatc atgcggcgaa atggttgtca
     1381 aagccatagt gactccttac ggacaataga catttttcag ggtggcgata atggcattta
     1441 ccgcctcatt tttaatggaa tatagaatgc gttgggcatc ccgttggctg ttgataagcc
     1501 cttcgtcccg catacgagcg agatgctgtg atgtcgcaga ggcgctcagt ccggtaatgc
     1561 gcgtcagctc tcccgcgctg gtgccggggg aaccgctaag catgcacaga atcagcaacc
     1621 gtttagggtg gctcattgct ttcaataagg ccgctgcctg ttcggtactg gcctgtaatt
     1681 gcgcgagttc agtcatattt atttaagtat tttctaaatt aagtaaactc taaactaaaa
     1741 atgcaacata taccagcctc agcagcgtaa atgagagtaa aagcgtaagc t
//
LOCUS       DAZPAB010000381         1788 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZPAB010000381 DAZPAB010000000
VERSION     DAZPAB010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.385"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..1134)
                     /gene="pdeF"
                     /locus_tag="ACHUPG_004271"
     CDS             complement(<1..1134)
                     /gene="pdeF"
                     /locus_tag="ACHUPG_004271"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR0863602.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
     gene            complement(1316..1468)
                     /locus_tag="ACHUPG_004272"
     CDS             complement(1316..1468)
                     /locus_tag="ACHUPG_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863603.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            1486..1677
                     /locus_tag="ACHUPG_004273"
     CDS             1486..1677
                     /locus_tag="ACHUPG_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR0863604.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          564 a          405 c          365 g          454 t
ORIGIN      
        1 ttgaatccgc agcatgatgc catagttctt aaccagcatt tccatgccag ggatgcgtaa
       61 ataacaaagc gcagaccagg gggcatcacg caacgcgcga ttcagggcgc gaacatttgg
      121 cagatgaacc accgggtcca catacgcaag ccgctgaatg cgtctgacta ccgctcgctg
      181 acgagttgcc agtacagcca tataattgac aataaaagag aataccagat aactggagga
      241 ggttatggtc agctgcgtgg tatagccagg ataaatggga atgtaatttt gatagctgtg
      301 gatgctgatc atcaacacga ccgcccagag cagcgaaatc agcttataac catagcgcat
      361 tgctccccac atcatcaggg gcagcaataa tgacaaggta taattggtgc taaaaattgt
      421 gcttttttca tttaacggca tgcataacag caacaataaa gcacctaatg ccagtagcca
      481 gagcgcgaac tcttttttgg tgactttggc atcaacctgc tgttttaatt gcgaataata
      541 gctacgtaaa taaaatggat ttcgcactac ccgaatgatg aagtagcaca gcgggacacc
      601 gatcagatta cccaccagta aggcctgata attgattaaa gtcccgaggt taaaaggcat
      661 gacaccgacc agattttctc tgctcgccag taatcctaca aatgcagcaa actggaaaag
      721 tatcagaaac agcgttgcag gaaacactat ctgccagaat atacgctggg aaattaaacg
      781 ggtatcacca tgtgaaacgt tgttacgccg gggagtaaag accctgtaac cgccccaaca
      841 gagaatgata atgataataa aattagccgt taatgagagc gtttcgtaaa aacccagctc
      901 tgcatatttg cggacaaaaa tccctaaggt gatccctggc aatgccgccc agctgaaaaa
      961 catcatcatg ctgatcatca gtgccaaagg cagataaaag agaaaaacct caccggaaga
     1021 aatatgcgcg aaagtattaa tgtgggcgaa aatgggcaag attaaagaag gcaggaacag
     1081 cggaagcccc caccatttat cacgtatttt tatataagtt gcattcagtt tcatagatgc
     1141 tcagcagaat cccccacatc ctgaaggagg tgtattcaga caggcatccc acctgacttc
     1201 gaatgatgat tattcatcac tatagagagc attgattcta agtgtcatat gaaagttcca
     1261 attgatatat atcaaacaaa ataaccctga ttaatgaatt attacgttta tcatgttaat
     1321 tcatcattat tacatcatca ttgtaaataa ttaaattaac ttccataaca ttaaaatatg
     1381 tatccactga cgctttttta cataacgaag aattgaccat tttgtcctgt tgtgccttaa
     1441 tgtaagtacc gtccacagcg tgggacatac ttcaaggaac cttttgtgag tcaggcaact
     1501 agtatgcgaa aacgacaccg atttaacagt cgcatgaccc gtatcgtact gctcatcagc
     1561 tttatcttct tctttggccg ttttatctac tcgtccgtcg gtgcctggca gcaccatcag
     1621 agcaaaaaag aagctcagca atccacactc tccgtcgaat caccggtaca acgttagcgg
     1681 ttaccttctc cactttcaca gaacataacg gcacttcgct gtcggatgct tttgctcttt
     1741 gggattatca aagcggcaga tattctttca tcttaaattt tacgtctt
//
LOCUS       DAZPAB010000382         1760 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZPAB010000382 DAZPAB010000000
VERSION     DAZPAB010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.386"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(1..264)
                     /locus_tag="ACHUPG_004274"
                     /pseudo
     CDS             complement(1..264)
                     /locus_tag="ACHUPG_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(379..1017)
                     /gene="ospI"
                     /locus_tag="ACHUPG_004275"
     CDS             complement(379..1017)
                     /gene="ospI"
                     /locus_tag="ACHUPG_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HFR0863605.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1164..1349)
                     /locus_tag="ACHUPG_004276"
     CDS             complement(1164..1349)
                     /locus_tag="ACHUPG_004276"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR0863606.1"
                     /translation="MLPLSVNLMSSGASLAARLQQHWLWYSYNTKTGGVLAYTFGPRN
                     DETCRELLVLFTPFCIY"
     gene            complement(1374..1646)
                     /locus_tag="ACHUPG_004277"
     CDS             complement(1374..1646)
                     /locus_tag="ACHUPG_004277"
                     /inference="COORDINATES: protein motif:HMM:NF024169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /protein_id="HFR0863607.1"
                     /translation="MATVTVHCPRCHSDEVYRHGRSCSRHERFRCRSCKRVFQLTYSY
                     EARKPGVKEQIVEMAHNGAGGRDTARTLKIGINTVIRTLKSSRPGG"
BASE COUNT          502 a          434 c          319 g          505 t
ORIGIN      
        1 cagcaagcgc tgtaactctt ttatttgttt tatagcggca gccagttctg aggcaggaac
       61 gacatcctct ccggcagcaa cagcagtgag acttccttcc agatattatt ttttccactt
      121 aagcaacagg ctgggctgaa taccatgcaa acgcgccaca tgggacaccg tcatgccagg
      181 ctccattgtc tgctgaataa tggcaatttt ttcctgagga gttttacgtt tacgaagctc
      241 cagccccagc aaagttccag tcatctcaaa attagaacta gtgttagaca tatatccaag
      301 cctatctctt attttaagat acatccactg tctggcgttt caaggggccg gctcaaatct
      361 atgtacaggc tctcagcatc agcaaagcct cttacttttc caactactac tgttagcata
      421 gtgatcaaat tcctgatcag atacatgaat tacctgaacc gaactatttt catctacaaa
      481 tgcatctggc gatttttcta caggatcata aagattaaat ccctttcctg tttgaacatc
      541 caaaaacata attttttcac catcgttaaa tccagctatc caatgagtgg cctgttcaat
      601 agaaatcatt actacacttc cttttgaagt attttgtaaa atattttctg caatacgctc
      661 acgaaattta ctataagttt cattaggatg tgacactatg ccttcactat caatttgctc
      721 taaatgccta tgaaaaaatc cggtcatata ctctgatatt tcattaggta cgggagcctc
      781 tcttactcca taccctaaca tagccataca agcatgaagc gcacaccctg aacagttacc
      841 atccccacca gacggattta tcattttctg taaagtcgct gccaattcag aatctttatc
      901 catatcacca agacttttag agtttaaaga ttccagcaat tttttaacat ttacatttcc
      961 ctcctctgta tgtgcctcat atccagcagt tccctgtaac gacaccccat taatcatatt
     1021 gtatcctgac agtatatata ctcactatat tttcttcata acaaatatcc ctctgatatg
     1081 acaacaaaaa acggagccgg attcctgttt ttgtgaactg cagctattcg tgtctaaaaa
     1141 gtgatccact taccaccaac ggcctaatat atacagaacg gagtaaacag gactaacagt
     1201 tcccgacagg tctcatcatt ccgtggcccg aaagtgtaag ccaacacacc gccagtttta
     1261 gtgttgtacg aataccagag ccagtgttgt tgcagccttg ctgccaacga agcgccactg
     1321 ctcatcaagt tcacagataa gggcaacatc agcgtgagcg actggcgcag atgttatccg
     1381 ccggggcggg agctttttaa tgtccgtatg acggtattga tgccgatttt cagtgtcctg
     1441 gcggtatcac ggccccctgc gccgttatgt gccatttcaa cgatctgctc cttaacgccg
     1501 ggttttctgg cttcataaga gtaagtgagc tggaaaacac gtttacatga gcgacaacgg
     1561 aaacgctcat gccgggaaca actacggcca tgtcgataaa cttcatcgga atgacagcgc
     1621 ggacaatgaa cagtaacggt agccatggaa aaccttaaat accagtagat taccagaaac
     1681 ttctgtcgat tgaagacatc acccgaagtt tgaattcatt cgaatacttt ttacgaggtt
     1741 cttttcgcca gtcctgtaat
//
LOCUS       DAZPAB010000383         1749 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZPAB010000383 DAZPAB010000000
VERSION     DAZPAB010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.388"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            454..1302
                     /locus_tag="ACHUPG_004278"
     CDS             454..1302
                     /locus_tag="ACHUPG_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR0863608.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
     gene            1319..1600
                     /locus_tag="ACHUPG_004279"
                     /pseudo
     CDS             1319..1600
                     /locus_tag="ACHUPG_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
     gene            1636..>1749
                     /locus_tag="ACHUPG_004280"
     CDS             1636..>1749
                     /locus_tag="ACHUPG_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004998486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863609.1"
                     /translation="MAGIRSTLIKEKNMNTNYEATVATTDNIVHEVYLEGKH"
BASE COUNT          500 a          398 c          411 g          440 t
ORIGIN      
        1 agcattatat agttctactc tggcgagata cgttggcata ttcatttcct tactggttgt
       61 gtgagaactt cagtaaagat accaccaaag cccggaagtg gtgaaataaa accgggcaca
      121 acacgaaggc gcatttccgg tatccataaa gagtcggtct tgtctgtgaa atttaaatgg
      181 tgggagtgcg cttccggttg taaataacga cattgctgtg tgaagtacca gttggcggca
      241 tcggtttaat tgctggctga tgtccgccct ttttaaagtg aattttgtga tgcggtgaat
      301 gcggctatgc gcacgcggca cagttaaaag tcatgttagt ccttattggt ttgggtggga
      361 aagccaactg taattgttaa ctggttaacg tcacctggag gcaccaggca ccgcatcaac
      421 aaagttcact tcggtgatga aaggaaagag aaaatgttga atgtagctat tgaaaaccag
      481 aacgggtgga attatagtgc acctgcacct cataaaacgg gtgccgggat tgctaccccg
      541 acgatgacta ctgcgcataa ccgcgctcag gcggtttttt tatgcgtcaa gcacagccac
      601 attcagatta tggtggggcg tgcagggcag ccgcaaggct ggccggtttc ggtagtcacc
      661 ggttgtagca accctgtgcg tctcaccacc catgagattg ctacctctgg tggtgagtca
      721 tttaaactga ctatcgaggc tgccatcatg gctactatcc ttaccctttc tcaccctgac
      781 gcaaccatcg aaaatggtcg cgctgtcact acgtctgttg cggttgcgga gttcttccgg
      841 aaaatgcaca aaaacgttat tcagaagatc gaaactctcg aatgctcgcc ggagtttaac
      901 cggcttaatt ttaagcccgt cacctatacc gacgccaaag gcgaaaaacg cccaatgtac
      961 caaatcacca aaaacggctt cgttttcctg gtgatgggct tcactggcaa aaaagccgcc
     1021 gcattcaagg aagcctacat tgctgagttc gaccgcatgg aggccgaact gcgccagaat
     1081 aacaccccac ctgcagacaa gatgattccg ggtgatggtc gcactctggt tgttcacttc
     1141 gacaaattcg gcaatgtcga attcaccgaa accgttcctg atggcgcact ggtctgtacc
     1201 ctggagactt tccggtttta tctggaaaaa cagggatgga ctcttgttaa ccggggcgca
     1261 attaaaaata tgactgtgga acaattactt tctttaaaat aaaaatggag ctacggatat
     1321 gaaccctatg cttgattatt gttttacctc tgcacctcag cagaccataa cacacaccgg
     1381 aatcaaaaat ttgttatgga taatcaaaaa aaacctccag tagaaaggtg atcccgattt
     1441 aattccctgt gcttatggcc tgtgtgacag acaagtaagc cctgataagg cttacactgc
     1501 tgaacttatt tacatgcacg gaaccatgat gaggaaaaag aaacgcaaat attgtagtga
     1561 aatctgcgcc gaaaaagacc agatggcaca cgaactttaa ttaactgact attcgaaact
     1621 gaatttatgc cagcaatggc agggattcgc tcaaccttaa ttaaggagaa aaacatgaac
     1681 accaattatg aagccactgt tgcaactact gataacattg ttcacgaggt ttacctggaa
     1741 ggaaagcat
//
LOCUS       DAZPAB010000384         1746 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZPAB010000384 DAZPAB010000000
VERSION     DAZPAB010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.390"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            311..505
                     /locus_tag="ACHUPG_004281"
     CDS             311..505
                     /locus_tag="ACHUPG_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863610.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            866..994
                     /locus_tag="ACHUPG_004282"
     CDS             866..994
                     /locus_tag="ACHUPG_004282"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863611.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(1304..1640)
                     /locus_tag="ACHUPG_004283"
                     /pseudo
     CDS             complement(1304..1640)
                     /locus_tag="ACHUPG_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          521 a          388 c          374 g          463 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat
       61 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
      121 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
      181 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
      241 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
      301 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
      361 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
      421 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
      481 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
      541 gcatcgttat cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct
      601 ttgtttgccg gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg
      661 acattgtaat aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat
      721 cgttgacaat atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct
      781 gaagcatccg acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg
      841 gaactttgac aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg
      901 gaaaagtggt acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag
      961 cttgtcagaa aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag
     1021 tgcccggcca gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc
     1081 aggctatagg caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac
     1141 ggtctgaata acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc
     1201 gagcgctgat tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa
     1261 atatgaaaag atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc
     1321 gtttaatatt cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt
     1381 acgaccacag tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata
     1441 agcagttgct tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg
     1501 atttccacat tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc
     1561 tgaagttctt tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa
     1621 cccactgtac tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact
     1681 ctatacagga taattagagg tcagtttgat tattcacaat tcatcatcag cattttctat
     1741 ttctga
//
LOCUS       DAZPAB010000385         1744 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZPAB010000385 DAZPAB010000000
VERSION     DAZPAB010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.391"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..353)
                     /locus_tag="ACHUPG_004284"
     CDS             complement(<1..353)
                     /locus_tag="ACHUPG_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR0863612.1"
                     /translation="MNRHTQIRQSVLACLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLNPLGFNYQRDNEMA"
     gene            complement(350..940)
                     /locus_tag="ACHUPG_004285"
     CDS             complement(350..940)
                     /locus_tag="ACHUPG_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0863613.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            complement(927..1298)
                     /locus_tag="ACHUPG_004286"
     CDS             complement(927..1298)
                     /locus_tag="ACHUPG_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR0863614.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            complement(1310..1711)
                     /gene="gpFI"
                     /locus_tag="ACHUPG_004287"
     CDS             complement(1310..1711)
                     /gene="gpFI"
                     /locus_tag="ACHUPG_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR0863615.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
BASE COUNT          393 a          527 c          407 g          417 t
ORIGIN      
        1 gccatctcat tatcacgttg atagttaaaa ccgagtgggt tcagggtgtc gatgagtccg
       61 gaaagtgccg gtacatcatt cagggccggg aaaatggtgc tctccatcca catatccagc
      121 tctgaatccg gtgcctgtgc ccggatgaag acggcaatat gcagaacagc ctgccagtca
      181 tcttcatccg tcatttttcc ggtgtactga gcatcactca gccacaccgc cacggcaggc
      241 agttcctgcg catcaataaa tgccggaagc ccgtcaaaaa acgtggcgct gtctccacac
      301 tgttcccgaa ggcatgccag cacgctctga cggatttgtg tgtgtcggtt catttactca
      361 gatgtaacct cagttgttgt ttcagggcat accccagctg tttcggcatt tccgcagcaa
      421 tgatgcggtc gcgggcatct tcaaatgcct gtgtcagcgg tccggacagc gggattttca
      481 ctacctccac aggaagacga ttttttctta gcctgccctg atggtcatgc cctgttgcaa
      541 aacgcgcttc aggaagacac ctcagaacat gccagcggcc attcgccagt tgctgaacaa
      601 atgccccccg gaaaaagtat tttcccactc tcagtccttc accagcacgc cgccgtgttg
      661 tgttcagttt gatggcgggc ggattgcccc ggttaacgcg gatcctggcc gtcatttttc
      721 ctgacggact ggctttaaac acccggacac gctgacgtac cagtttcagg gggatccctt
      781 tcacctggtt atcgcccgca acggtattcc cggcaacctg ccgggtggca accgagaccg
      841 ctttctgtgc cacacggttt atcgcccatg cgctggcctg tggcaccata cgggtatcaa
      901 ggctgttcag attgcggatg gcattctcaa gccccttcat aatatcgtcc tgcccttacg
      961 tttcccggca gcccctcacg ccgcaaccgg atataactac atccgccatc atcaggggtg
     1021 atacgatcca cacagaaaga ctcacgacca atcatcagcg aatcattgcg acacagccgt
     1081 aaaatatctg ctgttttcac aaacagggat ggtaaagagt cttcaatacg cacaccgccg
     1141 gccaccagag aaatattttc aggatcatca aaaaccccgg aaatcatgat cccttttagc
     1201 cggcctgacg taatgaccgc tctaatcccc atattgcgaa taatggcctt gtctgcgaga
     1261 tcaagtgcaa catcaaacag attatcgaaa tcagccacgc tgcctcccgt tattgcattc
     1321 tggccaggcc acgttctgtc attccggctg ccacaccggc aaagacacgg aacgccattc
     1381 ccggcggcac aaatgccaga ggttcatccc gcgtggcatg aagtgcatcg gtatgcagcg
     1441 tcaccagtgc cacgactgta accagatcat ccatattctg aatcacggtc tccggctgcg
     1501 ctgatatcac ctcattttca tgtccggtca gcgcattttc cgggtgggca gacttatccc
     1561 ggatgtcagt gtcatccata ttggcaagtt cctcctccag ctcagccaca cgctgcgcaa
     1621 tatctgctgc gcttcccgag atatccggct cacgtcccag cgatgctgcc agttctctca
     1681 gacgtttcag gttatcgtct ttctttgcca tatctgttcc tgtccgaata aaaaacggga
     1741 gccc
//
LOCUS       DAZPAB010000386         1732 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZPAB010000386 DAZPAB010000000
VERSION     DAZPAB010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.392"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..143
                     /locus_tag="ACHUPG_004288"
     CDS             <1..143
                     /locus_tag="ACHUPG_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0863616.1"
                     /translation="AEVELATLTWVDWYNNRRLPERLGHIPPAEAEKAYYASIGNDDL
                     SA"
     gene            127..486
                     /locus_tag="ACHUPG_004289"
     CDS             127..486
                     /locus_tag="ACHUPG_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863617.1"
                     /translation="MICQPEFTDKTLSRKPGAVQKVNETDAEYLVRKFDELEAKCAAQ
                     ENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLEN
                     VNITHWAVMPKAPEAGA"
     gene            486..791
                     /gene="yfdT"
                     /locus_tag="ACHUPG_004290"
     CDS             486..791
                     /gene="yfdT"
                     /locus_tag="ACHUPG_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdT"
                     /protein_id="HFR0863618.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            707..1141
                     /locus_tag="ACHUPG_004291"
     CDS             707..1141
                     /locus_tag="ACHUPG_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0863619.1"
                     /translation="MGYDSRLDRLAATSWYPFLNNVTARGEIMEPYSLTLDEACHFLK
                     ISRPTAINWIRTGRLQATRKDPTKNKSPYLTTRQACIAALQSPLHTVQVSAGDGITEE
                     RKCHSSAEVKYGTPVSHCRTVKDLNSLLEQRTKGRRQNSMTS"
     gene            1063..>1732
                     /locus_tag="ACHUPG_004292"
     CDS             1063..>1732
                     /locus_tag="ACHUPG_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0863620.1"
                     /translation="MPNGKRFKQSLGTKDKRQATELHDKLKAEAWRVSKLGEIPDITF
                     EEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGIQLRDITESKIYSAMQKMTNRRHEE
                     NWKLRAESCRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIKV
                     PQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQINMQRRV
                     AWINP"
BASE COUNT          568 a          379 c          428 g          357 t
ORIGIN      
        1 gtgcagaagt ggaactggcc acactaacgt gggtggactg gtataacaat cgacgattgc
       61 cggaaaggct gggccacatc cctccggcag aagcagaaaa agcttattat gcttccatcg
      121 gaaacgatga tctgtcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg
      181 gttcagaaag taaatgaaac agatgctgaa tacctggtgc gtaaattcga tgagcttgaa
      241 gctaaatgtg cagcacagga aaacaaagta ataccagtgt caactgaact gccaccagca
      301 aatgaaagtg ttttgttatt cgatgctaac ggagaaggct ggctaattgg ctggcgttct
      361 ctctggtaca cctggggaca aaaagaaacc ggagaatggc agtggacatt tcaggtcggg
      421 gaccttgaaa acgtcaatat cactcactgg gcagtaatgc caaaagcacc ggaggctgga
      481 gcataatgac cacttttacc gacaaagaac tgattaaaga aattaaagag cgtatcagca
      541 gccttgacgt gcgagacgat attgagcgcc gtgcttatga aatcgcactc ctatctctgg
      601 aagtagaacc agatgaacgc gaagcttatg aattattcat ggaaaagcgt tttggtgact
      661 tagtagatcg tcggagagca aaaaacggcg ataacgaata catggcatgg gatatgactc
      721 tcggttggat cgtctggcag caacgagctg gtatccattt ctcaacaatg tcacagcaag
      781 aggtgaaata atggagccat acagcctcac actcgatgag gcctgtcatt ttctcaagat
      841 atccagaccg actgccatta actggatacg cacagggcgt cttcaggcaa cacgcaaaga
      901 tcccactaag aataaatctc cttacctcac aacacgacaa gcctgcattg cggctcttca
      961 gtctccgctg catactgtcc aggtgagcgc gggtgatggc ataacagagg aaagaaaatg
     1021 tcactcttcc gcagaggtga aatatggtac gccagtttca cattgccgaa cggtaaaaga
     1081 tttaaacagt ctcttggaac aaaggacaaa aggcaggcga cagaactcca tgacaagcta
     1141 aaggctgaag catggcgggt cagcaaactt ggtgaaatac ctgatataac gttcgaggaa
     1201 gcgtgtgtca ggtggcttga agagaaagca cataaaaaat cactggacga tgacaaaagc
     1261 cggatcggat tctggcttca acatttcgca ggaatacaac taagagacat tactgaatca
     1321 aaaatttatt cagcaatgca gaaaatgacg aaccggcgtc atgaggaaaa ctggaaactc
     1381 agggcagaat catgcagaaa aaaagggaaa cctgttccag aatacacgcc aaaaccagcg
     1441 tccgttgcaa cgaaggctac gcatctttca tttataaagg ccctactaag agccgcagag
     1501 cgtgaatgga aaatgctgga taaggcacca attattaaag tgcctcaacc aaagaataaa
     1561 cggatccgct ggctggagcc ccatgaagca caaaggctga ttgatgaatg tccggagcca
     1621 ttaaagtctg ttgttgaatt tgcactggca acaggcttaa gacgctcgaa catcatcaac
     1681 cttgaatggc aacaaataaa tatgcagcgc cgggtggcat ggataaaccc gg
//
LOCUS       DAZPAB010000387         1717 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZPAB010000387 DAZPAB010000000
VERSION     DAZPAB010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.393"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(110..577)
                     /locus_tag="ACHUPG_004293"
     CDS             complement(110..577)
                     /locus_tag="ACHUPG_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFR0863621.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="ACHUPG_004294"
     CDS             complement(643..882)
                     /locus_tag="ACHUPG_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HFR0863622.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="ACHUPG_004295"
     CDS             complement(1017..1622)
                     /locus_tag="ACHUPG_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0863623.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          549 a          378 c          305 g          485 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata tttttgggat
      961 gtggctataa ttatctattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacattaag ttaagca
//
LOCUS       DAZPAB010000388         1705 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZPAB010000388 DAZPAB010000000
VERSION     DAZPAB010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.394"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            148..1248
                     /gene="ompC"
                     /locus_tag="ACHUPG_004296"
     CDS             148..1248
                     /gene="ompC"
                     /locus_tag="ACHUPG_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR0863624.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1412..>1705
                     /locus_tag="ACHUPG_004297"
     CDS             1412..>1705
                     /locus_tag="ACHUPG_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0863625.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTC"
BASE COUNT          515 a          342 c          346 g          502 t
ORIGIN      
        1 atcacatatc gataaataat attattctat ttttctcatt gtttttaatc accattctat
       61 tttaaaaaca gtatgtcatt tataaatgac gccgtttaaa attcgtttag aaaatacgtt
      121 taacgataat taaaaggatt ctgttaaatg aaattaaaaa tagttgcggt ggttgtaact
      181 ggtttgttag ctgcgaacgt agcacacgct gccgaagtct ataacaagga tggtaataaa
      241 ctcgaccttt atggcaaggt taccgctcta cgttatttta ctgatgataa gcgtgacgat
      301 ggtgataaaa cttatgcccg tctcggcttt aaaggagaaa cgcaaatcaa tgatcaaatg
      361 attggttttg gtcactggga atatgatttt aaaggctata acgatgaagc caacggctcg
      421 cgcggcaaca agacccgtct ggcctatgct ggtttaaaaa ttagtgaatt tggctctctg
      481 gactatggtc gtaactacgg tgtcggctac gacattggtt catggaccga tatgttgcca
      541 gaatttggtg gcgatacctg gagtcagaaa gatgtcttca tgacataccg taccaccggt
      601 ctagcaacct atcgcaacta cgatttcttt ggcttaattg aagggctgaa ctttgccgcg
      661 caatatcaag gcaaaaataa acgtactgac aacagtcatc tttatggtgc tgacgacacg
      721 cgtgccaacg gtgacggttt cggtatctcc tcaacttatg tttatgatgg ctttggtatc
      781 ggtgcggtat ataccaaatc cgatcggaca aatgcgcagg aaagagccgc agctaatcct
      841 ctcaatgcct ccggtaagaa tgcagaacta tgggccacag gtataaagta tgatgccaac
      901 aacatctact ttgcagctaa ttacgatgaa acattaaaca tgaccaccta tggggatggt
      961 tatatctcta acaaagcaca aagttttgaa gtggtggcgc aatatcaatt cgacttcggc
     1021 ttgcgcccat cactcgctta cctgaaatcg aaaggcagag atctgggccg ctacgccgac
     1081 caggacatga ttgagtatat cgacgttggt gcgacgtatt tcttcaacaa aaatatgtcg
     1141 acctatgttg attataaaat caacctgatt gatgaaagcg actttacccg tgccgtagat
     1201 attcgcaccg ataacatcgt cgctacgggc attacctatc agttctaacg tcctctcctg
     1261 accagccctt ctctgggctg gtttttgatt taagtgctgc tgtatgactc cttaagtaaa
     1321 aatgagcaat tcttgatatt tctggtcatt cacctcaatt aatattattc catatcctcc
     1381 ggggacatct tactataata tttattgatg agtgattatg accaatatta atacagcttg
     1441 tgtaaaaaat aatgccagtt atcaacttaa taacgcatta cctaacaaag aaaccatctc
     1501 cagcaatttt tgtgaacgac tagaacaatg gggtaataaa tcggttaata atggtgaaga
     1561 aagagcaatt gccgtggagc gaattaaaga agcttacaat tcgaacatgg catctcttga
     1621 tttgtcatat cttgatttga gtgaattacc gcctatcccc tcgacagtta atacgttaaa
     1681 tctggaaaac aattgtctaa cttgt
//
LOCUS       DAZPAB010000389         1699 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZPAB010000389 DAZPAB010000000
VERSION     DAZPAB010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.395"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            41..1525
                     /gene="icsB"
                     /locus_tag="ACHUPG_004298"
     CDS             41..1525
                     /gene="icsB"
                     /locus_tag="ACHUPG_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HFR0863626.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            1538..>1699
                     /gene="ipgA"
                     /locus_tag="ACHUPG_004299"
     CDS             1538..>1699
                     /gene="ipgA"
                     /locus_tag="ACHUPG_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HFR0863627.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHE"
BASE COUNT          661 a          314 c          258 g          466 t
ORIGIN      
        1 acatccccac aatcaccaag taatggagag ttaataaagt atgatcctca aaattagcaa
       61 tttcattgac gcaagcaata caaaagggcc tatacgcgtt gaagatacag agcatggacc
      121 tatattgata gcacagaaat tcaacctaaa ggatctcttt ttcagaacat taagcactat
      181 taatgcaaag ataaattcac agatattaaa tgaacaacta aaaaattatc gactagagaa
      241 tcaaaaatcc ttattgcttt tcctcaatac attagctagc gaaaaatctg cagaaagtgc
      301 gtttgccgcc tatgaagcag ctaaaaactc aattcaacac tctttcacag gtagagacat
      361 caaattaatg ttgaataccg cagagcgttt tcatggcatc ggtacagcca aaaatcttga
      421 aagacattta gtttttcgtt gctggggaaa tagaggcata acccatttag ggcatactag
      481 tatcagtata aaaaacaatt tactacagga accaactcat acctatctca gctggtatcc
      541 cggcgggaat gttacaaaag atacagaaat aaactacctt tttgaaaaac gctcaggtta
      601 cagtgtcgat acctataagc aagacaaact aaatatgatt tcagaccaaa cagccgaaag
      661 acttgatgca ggccaagagg ttagaaactt attaaattca aaacaagatc aaaataacaa
      721 caaaaaaata ttttttccca gagctaatca aaaaaaagac ccctatggtt attggggggt
      781 cagtgctgat aaagtataca ttccattatc aggtgataat aaaacaaagg atggtaaaat
      841 cagccataac ctttttggcc ttgatgaaac aaacatgtca aaatttatat gcaaaaagaa
      901 agcagatgct ttcagacaat tggcaaatta taaattaatc agtaaatctg aaaactgtgc
      961 tggtatggca cttaatgttc ttaaagcagg aaattctgaa atatactttc cactccctga
     1021 cgttaaattg gttgctacac ctaacgatgt atacgcgtat gcaaataaag ttaggcaacg
     1081 cattgaaagt ctcaatcagt cttataacga aataatgaag tatattgaat ctgattttga
     1141 tctttccaga ttgactcaat tacgacgcag ctatcttaaa agtttcaata aaattaacct
     1201 tatccacaca ccaaaaacat tcaagccttt atcaatttca ctttacaaac atcctactga
     1261 aaatgtatct tcagaagact ttgatgcggt catcaacgct tgccactcat acttagtaaa
     1321 atcagcaccc agcaatatga ctcgtgtatt aaacgaatta aaaaccgaag caacagataa
     1381 aaaagaagaa atcatcgaaa aatcaataaa aattattgat tattacaatt ctcttaaatc
     1441 gccagatctt ggaaccaaac tttacattca cgacttatta cagatcaata agcttttatt
     1501 gaataactct cattctaata tatagaaggc catagaaatg tgtcgcaaac tatatgataa
     1561 actttatgaa ataacaggtg caaagcttga ttttaacgat aaaaatcaag cctttatatt
     1621 gctcgaagaa caaatacctg tttgtataac agataatgat gaatacatct ttttgaccgg
     1681 acttcttaat gaacatgaa
//
LOCUS       DAZPAB010000390         1644 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZPAB010000390 DAZPAB010000000
VERSION     DAZPAB010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.396"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            41..1438
                     /gene="ccp"
                     /locus_tag="ACHUPG_004300"
     CDS             41..1438
                     /gene="ccp"
                     /locus_tag="ACHUPG_004300"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR0863628.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT          446 a          328 c          426 g          444 t
ORIGIN      
        1 tttctgggtc acaataactc accaccgtga gggaagaata atgaaaatgg tctcacgtat
       61 taccgcgatc ggcctggctg gcgtcgcgat ttgctattta gggttatctg gttatgtgtg
      121 gtaccacgat aataaacgca gtaaacaggc cgatgttcag gcatctgctg tcagtgaaaa
      181 taataaggtt ttaggctttc tccgcgaaaa aggatgcgac tattgccaca cgccttcggc
      241 agaattaccc gcctattatt atattcctgg cgcgaaacag ttgatggatt acgacattaa
      301 gcttggatat aaatctttta acctcgaggc cgtgcgtgcg gcactgctgg ctgataaacc
      361 cgtttcgcaa agcgatctga ataagattga atgggtgatg cagtatgaaa ctatgccacc
      421 aacgcgttat accgcgctac actgggcggg taaggtgagt gatgaagagc gggcggaaat
      481 actggcctgg attgcaaaac agcgcgcgga atattacgcc agcaatgata ctgctccgga
      541 gcatcgcaat gaaccggtgc agcccatccc gcaaaaactg cctaccgatg cgcaaaaagt
      601 ggcgttgggc tttgcgctgt atcacgatcc ccgtttatcg gctgatagca ccatttcatg
      661 cgctcattgc catacgttga atgcgggggg cgtcgatggc agaaaaacat cgattggtgt
      721 tggtggcgca gttgggccga ttaatgcgcc gacggtattt aactcagtat ttaacgttga
      781 gcagttctgg gatggtcgtg cggcaacatt gcaggatcag gcaggtggac cgccgttgaa
      841 cccgattgaa atggcgtcga aatcctggga cgaaattatt gctaagctgg aaaaagatcc
      901 gcagcttaaa gcgcagttcc tcgaagttta tccgcaaggt ttcagtggcg aaaatattac
      961 tgatgccatt gctgaatttg agaaaacatt aattacgccg gattccccat ttgataaatg
     1021 gttgcgtgga gatgaaaatg cgctgacggc gcaacagaaa aaaggctatc aattatttaa
     1081 agataataaa tgtgcaactt gtcatggtgg tattattctc ggcggacgtt cctttgaacc
     1141 gttggggctg aaaaaagact ttaactttgg ggaaattacg gcggcggata ttggtcgtat
     1201 gaatgtgact aaagaagagc gtgataaatt gcgtcagaaa gtacccggtt tacgtaacgt
     1261 tgctttaacg gcaccgtact tccatcgcgg tgacgtgccg acgctggacg gggcggtgaa
     1321 actgatgctg cgctatcagg taggcaaaga gctgccgcag gaggatgtgg atgatatcgt
     1381 agctttcctg cacagtctga acggggtgta cacgccgtat atgcaggata aacaataatt
     1441 aatttgatcg cccgaacagg aatgtttggg cgatttttat tacgataata aagtctgttt
     1501 ttaatattat catgttaaat gtttatatta taaaaagtcg tttttctgct taggattttg
     1561 ttatttaaat taagcctgta atgccttgct tccattgcgg ataaatccta cttttttatt
     1621 gccttcaaat aaatttaagg agtt
//
LOCUS       DAZPAB010000391         1624 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZPAB010000391 DAZPAB010000000
VERSION     DAZPAB010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.397"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..598)
                     /locus_tag="ACHUPG_004301"
     CDS             complement(<1..598)
                     /locus_tag="ACHUPG_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863629.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLA"
     gene            complement(1182..1624)
                     /locus_tag="ACHUPG_004302"
                     /pseudo
     CDS             complement(1182..1624)
                     /locus_tag="ACHUPG_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          557 a          329 c          295 g          443 t
ORIGIN      
        1 gagcaagaac ataataccaa ctattattat aacaaagcat ctgatattct ttttttaatt
       61 gaggtttata ccagccaaca agaaaggaaa aaaaccaaaa ataaaccaaa aaaccagcca
      121 taattaactt aatgcggtgt tctctaacaa aagggatatc tgaaaaacat gatacagaaa
      181 agatccgtcc atatatacga attattattg aaataaccaa tgaactaata atgaatgaaa
      241 gtgataatgg aagtatgaaa tattgtttga gaattaaaag ttcgaatatt acagggaaaa
      301 acagtagggt aatagcaaaa aatagtctaa aaaaactcaa gtcttgtatt ttacttcttt
      361 tctttataag aagaaaaaat aaaataccaa tcccccaacc ggtaaaaagt atacccatta
      421 ctatgatagc atagagtaaa cttctggcaa catcatccaa acctgcacca acaacccacc
      481 atggaaatcc atagtaaaaa gaggttcccc agccataaga ataagcactg acccacccta
      541 cacatcccat ataggcgatg aataatgaag aatttctaag caatgaattg tcattcataa
      601 gaaactaact gataatgtaa aaaatattcg agataaaagt tgatctttta attttcatgc
      661 taggaaatta acaataatat cattgttaat gcatatttat catatcaaaa tactctgcag
      721 aatgctacta tcaaaaccag acaagtgaac aaaacttggt caacatttag taacgctagt
      781 tggtaatgtc ttcaatcgat agaagtttct ggtaatctac tggtattcaa acagttaggg
      841 ctcatacagc ttgctggacc ttccccataa ccgcccgaac gtgaaggtct gatgtgttgc
      901 aagagagccg gatgcgggaa atctgcaagt ccggttcgat gagagggacg ttgtttttac
      961 ttgttgttga tgaagacttt cattattgaa cagaatgata atattgtcta aacaaaacat
     1021 taaatctcat aattctttgt agaaaacgct tttcttgaaa ttaaaaataa gtagaatcag
     1081 cagtctcatt ataataacag acaatgtcat gctctcgccc gttccggtaa taaacggagc
     1141 gggctatctt tttggaatat ttatggccca caatccagtt ttcataaata cgattcattc
     1201 cattcttcgt tttttctgag cattgcgttc ataatggtca gcaacttacg catacaggca
     1261 accagtgcca cttttttggc gtttccggct gcaagtaggc gcatgtaaaa cgcctttatc
     1321 accagattaa accggggggg ggcctcaagc gccgccatgt acagagctgt tcttccccag
     1381 cacaatcacc gaaaatggtt cgtcgaccgc gcagagttcc cgagtcccgg tttacctgag
     1441 caacaccgac aagtgcgctg atttcacgtc tcgagaggct gctcgattca ggcatttccg
     1501 ccagcaatgc tgcggcagtc atggctctaa cacctcagac gttcagtaag ctctttaaag
     1561 taactctgaa tgtgcctgtt catatctggg gcttccccga tcatggtggg aaggcttaga
     1621 acgc
//
LOCUS       DAZPAB010000392         1608 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZPAB010000392 DAZPAB010000000
VERSION     DAZPAB010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.398"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(6..509)
                     /locus_tag="ACHUPG_004303"
     CDS             complement(6..509)
                     /locus_tag="ACHUPG_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR0863630.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
     gene            complement(554..1517)
                     /locus_tag="ACHUPG_004304"
                     /pseudo
     CDS             complement(554..1517)
                     /locus_tag="ACHUPG_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT          451 a          352 c          330 g          475 t
ORIGIN      
        1 atccattaac tggcctcctg cgcaggttgt ttcagttgtt gaatgctttg attaataaaa
       61 cacaaggcat aaaacatcat taccactcca cttaatggca gaatgatata tacgtatccc
      121 atcggtattt gcatagcggc agatacctgt gatgttgcca gtgttttatc aattaatttc
      181 aggccaccag tcacaatgac cgaaccggca aaacctaaaa tgagaacgtt aatgactata
      241 ctaagaagta attgtttgcg cttattcagg gcgagagcaa ataaatcaat agagagatgt
      301 cgctgcgcgc cgacggtata tgcagcacct aataatccta cccagatcat caaaaagcgt
      361 gccagctcat cggttaatgt acttggctgg ttaagtacgt agcggctaaa tacttgccag
      421 actacgcata caaccagagc aagcattacg gcgacagaaa acgtggcaat gacgcggtct
      481 accgcaagtt tcaggcggtt aaatatcatt gtaagcatcc tggaaaatca gggccagagg
      541 ctggcccgga atattattga gcagcgttgt cgaatttctc taacaaagca gcttgttttg
      601 gatctttttt gaagtcgtca aacagaggtt gcacggcttt acggaatggc gctttatcca
      661 cttttacaat ttcacccccc atagctttcg cctgagcacg tgtgtcagcg tcaatttttt
      721 cccacaattt ttgttgataa gcttcggatt tcttcgccgc tgtttcgagg atttgttgct
      781 gttctggggt taatttattc catgttttgg tggagataac caaaaagtca ggaattgagg
      841 cgtgttcatc ttcagaaaaa actttggcaa tttcaatatg tcgagtctgc atccaggaag
      901 gcacgttatt ttctgcgcca tctactacac cttgctgcat cgcagtataa acttcgccaa
      961 atgaaattgg agtgggtgaa ccacccatca gttcaatcat tttgatggtg gtcggacttg
     1021 gctgaacacg aattttcagt cctttaagat cttctggctt tgtaatcggt tttttcgcat
     1081 agaaactgcg agtacccgct acataagctg ataaagcaaa aaagcctttg tcttttgttg
     1141 agtccataat ttctttacca acttcaccaa aaacaactta ataagaacgg caaattatag
     1201 atagcataaa tattatcaaa agactccaga tcactggcgg aacctttggt catatccagt
     1261 gcgccatttt gcaacaattc catagtttct cgcgcattac ccatctggct gctgggataa
     1321 atacggatca ccatattacc ttttgacagc tgttttacct ctttcgccag ctcttcaaaa
     1381 ctctgatgta caacatgact tctttccagg ttgtgtgcta actttaacgc cactttttct
     1441 gcggcgttta cgccagtgct aaccagagcc agcaaggatg cgcagactaa tgagcgggta
     1501 aatgacctca ttttcataaa aaaactccct gttgaaggat ggaaattggt tgatatctaa
     1561 ttgtgcttat cagtgatttg tgcgacaaat tggttaacct gtctgaca
//
LOCUS       DAZPAB010000393         1606 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZPAB010000393 DAZPAB010000000
VERSION     DAZPAB010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.399"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            91..465
                     /locus_tag="ACHUPG_004305"
     CDS             91..465
                     /locus_tag="ACHUPG_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFR0863631.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            528..1466
                     /locus_tag="ACHUPG_004306"
                     /pseudo
     CDS             528..1466
                     /locus_tag="ACHUPG_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT          475 a          218 c          315 g          598 t
ORIGIN      
        1 atttttatta taggcgttgt agttttatgg gtattttcta tagaaaatat ccaaattagt
       61 aataacctca cttctggagc aaaataaagg atgttaaaaa tagggaagtt attgaccagt
      121 tcttttttta gttatttttt aattggaatt gtcaatactg ctttacattg gggggtattt
      181 tatgcgtgtt ataataattt agcttttggt caaggccgaa gtaacattgt tggttttata
      241 tgtgcagcaa ccttttcttt ttttgcaaat gcaagatgct catttaaagt atctgcaacc
      301 aaggctagat attttatatt tatatttttt atgggggcga tgagttattt attcggcgtt
      361 ttatttgatc tcttagcttt atctccaata tttacattgt tcaccttttc tctttttagt
      421 ttggttttag gatattgcgc atcaaaatat ttcatttttc gttagtaaac gatttgttaa
      481 aaacacaatt acttgcacat tagctaaata gtttatatga ggggcttatg tatcactctt
      541 ttaatatcgc tgtaattatt ccttgttata atgagcagaa ggcaatagca aaagtcataa
      601 atgatttcaa aacgaatatt ccaactgctt ctatatatgt atttgataat aattcgactg
      661 acagtacagc acaagtggca gaggatgctg gagcaacagt tcactctgtt cctctgaaag
      721 gcaaggggaa tgtcgtaaga cgtatgttct cagatgtaga tgcggatatt tatctaatgg
      781 ttgatggaga tgacacttat gatgcaagtt ctgctccaga aatgattaac catctcataa
      841 aaaatcaatt ggatatggtt gtaggatgtc gtcaagagaa tggagatcaa aatacataca
      901 gaaaagggca tcgctgagga aataaattgt tgactggtac tgttcagact atctttaaag
      961 ggaattttac tgatatgttg tcggggtatc gtgtattttc cagacgctat gttaaatctt
     1021 ttccatgtct atcaagggga tttgaaatcg aaactgagct tacaattcat gctttagaat
     1081 tacgtatgaa gtatggtgag gttaatacaa aatatggcga gcgatctgaa gggagtgtga
     1141 gcaaactatc aacttggagt gatgggttca agattctgaa aacaataatt aaactttact
     1201 cgcttgaacg gccactatac ttttttagta ttatcggcgt tttacttgct gctttgtcta
     1261 taattttagg attgcccatc attgttgatt atattgatac aggtttggtg agaagatttc
     1321 cgactgcctt tttaactgcc tccatcatgt tatcgtcaat aatggcattt gtatgtggca
     1381 ttattttgca ttcaaataca acaactagac gtgaaatgaa ggcattattt tatctttctg
     1441 aaaaaaacta taaactgata atgtaaatag agtattttac tatgattgaa ttgccgttgt
     1501 atttgtatgt tgtgtattgt tttgcttttg gaatgacatt ttttgtcatt ggtaatattg
     1561 tattgtcatc tttttttaag gagggggtgg caggacttaa caaatt
//
LOCUS       DAZPAB010000394         1602 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZPAB010000394 DAZPAB010000000
VERSION     DAZPAB010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.400"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(334..480)
                     /locus_tag="ACHUPG_004307"
                     /pseudo
     CDS             complement(334..480)
                     /locus_tag="ACHUPG_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            534..716
                     /locus_tag="ACHUPG_004308"
     CDS             534..716
                     /locus_tag="ACHUPG_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR0863632.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(817..1146)
                     /locus_tag="ACHUPG_004309"
     CDS             complement(817..1146)
                     /locus_tag="ACHUPG_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR0863633.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1318..>1602)
                     /locus_tag="ACHUPG_004310"
     CDS             complement(1318..>1602)
                     /locus_tag="ACHUPG_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863634.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          435 a          368 c          377 g          422 t
ORIGIN      
        1 tagcaatgtc tgtcgcacaa caaccacaat gtcacaaata aaaaatgaga ataaccagtt
       61 ccctgaaggc gacgtagccc ggtacaagag taacccacgt tagcgcttgg cgacggcgga
      121 acaaagctga ttcgcgacgt aagaaagtgg caggctcata tgacggacct ctgtaatgcg
      181 gtatccaacc cgcgaatatc agcatgattc accgtcggat ttactgctac gtcgccctca
      241 gggaattaaa cctaaaaaga aaatcgtcaa aattagagat tccggtttga cacggaaagt
      301 catatcagcg tcaacggagc accgtattga cgcttattta ttggtgagaa ctacgttcca
      361 tggcaggagt tcgtcaacac ggttggaggg ccattcaggc agtatgctca gaatatggcg
      421 cagatacgct tccggatcga taccgttcag acggcaggtg cctgcttttc cggcaacaag
      481 caggaatctc agcgctgaag caggaattag aggtaaaaac gccctaccgg gccatgaatc
      541 acccggtcat tggagtagta accaaagcag atttagccag catggaacaa atatctttgg
      601 tgaaaagttg gttacgggaa gctggagcgc acaacgtgtt agtaaccagt gcagttaata
      661 acaatggagt tacagaactt tttgccttgc tgcatacaga agaaggctgt tgttaatttc
      721 gattggttct cagatgagaa tgatgacggt gatacacaga taagaatcaa tgagcaatga
      781 gggttgccgg gcaaccctca ttgaataaaa cgggaattac ttcgcttcgc cgtttttcat
      841 ttcgcccata gctttcagct ttttggagat atcgcggcgt tctttggaga gctcggcatt
      901 tttgatgatg taatcgtcaa cgcgatcttc atagtcacct ttcatgctgg cgatgatgcc
      961 ctggattgct tcaacgctca tccctggctt gatgtaatcg ctcaggttgt ccagcaacag
     1021 gacgcgcttc tggttgtcac ggatcttttt ctcaacgtcc tgaatttcac gttgcagttt
     1081 gttcttacga cggaacagac gaacaaattc cagtacgtcc tggaatgaag gcttggtagt
     1141 ttccattttt atacccctga taatgtgaga gtcggattcg tttaagcaac cgctattcgt
     1201 tagggccaac attactcata gctgccgcag atgacaatgc ttttatccct tttcactatc
     1261 atacccttta tccttgctga atcgaagcag cagcaagatg attctgaagt tcaggaatta
     1321 tttgcgcaag gcccggcaaa tcagattcga tagcaactca agctgatgtg ccgtttccat
     1381 gttcagccac acataaccat agattggtgt ttccacgact ttcaggtcat ggtggttatc
     1441 gagcaactga cgcagctctt ccacagcatc gttcaatttt tccgtattgg caaaaaccgg
     1501 ctgcggatta ccttcgtaca gcgcatgaac aaggctcagc agtatttgct gcatcatgtg
     1561 ctgggtatca caaagttttt gcgcgttcaa taacacgaaa tg
//
LOCUS       DAZPAB010000395         1597 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZPAB010000395 DAZPAB010000000
VERSION     DAZPAB010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.401"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(1..702)
                     /gene="lpfB-O113"
                     /locus_tag="ACHUPG_004311"
                     /pseudo
     CDS             complement(1..702)
                     /gene="lpfB-O113"
                     /locus_tag="ACHUPG_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
     gene            complement(750..1322)
                     /gene="lpfA-O113"
                     /locus_tag="ACHUPG_004312"
     CDS             complement(750..1322)
                     /gene="lpfA-O113"
                     /locus_tag="ACHUPG_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR0863635.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          474 a          337 c          319 g          467 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcctt taccatgaat catcacttat attaataagt
     1381 gtgtttttga ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta
     1441 attacgcaaa aataaaatat agatttattg tttaaatatt atttaaacag taaaaaatca
     1501 agtttgttat cagacattaa tgataattca gaatatattt atatagaaag atagatgaat
     1561 ttattgatgt cagttataaa aaagggccac cggtcgg
//
LOCUS       DAZPAB010000396         1587 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZPAB010000396 DAZPAB010000000
VERSION     DAZPAB010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.402"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..71
                     /locus_tag="ACHUPG_004313"
     CDS             <1..71
                     /locus_tag="ACHUPG_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863636.1"
                     /translation="RFLIFNTEQSGFYEINDVYVRK"
     gene            305..433
                     /locus_tag="ACHUPG_004314"
                     /pseudo
     CDS             305..433
                     /locus_tag="ACHUPG_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039289737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            623..1027
                     /locus_tag="ACHUPG_004315"
     CDS             623..1027
                     /locus_tag="ACHUPG_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863637.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            1090..1587
                     /locus_tag="ACHUPG_004316"
                     /pseudo
     CDS             1090..1587
                     /locus_tag="ACHUPG_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          453 a          375 c          411 g          348 t
ORIGIN      
        1 acagattctt aatatttaat acagaacagt cgggatttta tgaaatcaat gatgtttatg
       61 tcagaaagta ataatcctct cccacaaagt gaattaaatg aatatataat gcgttcgaag
      121 tatatgtatt gagctgtcat actgagaaac aaaccttagg gcagcacaat taaagccaga
      181 aacaactatg caagaaagtt actaaataag tgctcggaac agtaaaacac tcagggggga
      241 ctcagtccgg attatgtaat cagataaatt gccaaacatc acaaatcacc agccggactg
      301 agtgatgccg agcattgcac caataccccg tgatgaacga tgcctgatgc agaaagctcc
      361 ctataaaaca aagaacaaaa actacgaccg gcgattcacc gtcatactga tgctgcatcg
      421 tggtgacact gtctgtcact ttcatgttga ctgaacaact ctgctgcgaa gaaggtgtac
      481 cgccggcggg atttgattgt ccggcgtcta gcttccactt ccctgacgga actgaaagtt
      541 gttatggccg gtcagcttgc cctgtccgca ggacatgagg tgctgtgacg gacagcacca
      601 gtaacggaaa gtgacaatga aaatgaatac tgcgctgata gttgcactaa tgtgtatgtg
      661 gtatgcggta cccgctgctg caaaagagac tttactggct atgccacgta attcaacaga
      721 acattgctat gcggagatta atgtgcacgg accatatggg gtgtatttcc gagtggtccc
      781 gcatcccccc gggggaaaga gctgggtcga atgtaacagt gactactatt actctgataa
      841 accaccggga gtacagatac tcggaacccg tgcagggtgt cgggtatacg gaatctgcgg
      901 gaccacctcg acactgcatg tggctggaag aggggttgtg tgtattaaaa atatatgcag
      961 tccacgtggg atgataattc acaggatacg gaaacgtcct gtagtggctg taagcgatga
     1021 gatgtgatct tacccagcaa tagtagacac gcggctaagt gagtaaactc tcagtcagag
     1081 gtgactcaca tgacaaaaac agtatcaacc agcaaaaaaa cacgtaaaca gcattcgcct
     1141 gaattttgca gtgaagccct gaagcttgct gaacgcatcg gtgttgctgc cgcagcccgt
     1201 gaactcagcc tgtatgaatc tcagctctat gcgtggcgca gcaaactgca gcaacagatg
     1261 acctcgtctg agcgtgaaag tgaactgccc gcctgaagcg gcaactggcg gagcaggcag
     1321 aggaactggc tatcctccaa aaggccgcga catactttgc gcagcggctg gtatacgtgg
     1381 tgtcagcgtc ggacaggaat aagccctcgt cagcagttcc gtcagcactg cgacagcgtt
     1441 gtcctcgcgg cggcctttac ccggtcaaaa cagcgttacg gtgcaccacg cctgacggat
     1501 gaactgcgtg ctcagggtta ccactttaac gtaaaaaccg tggcggcaag cctgcgccgt
     1561 caggggctga gggcaaaggc ctcccgg
//
LOCUS       DAZPAB010000397         1581 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZPAB010000397 DAZPAB010000000
VERSION     DAZPAB010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.403"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACHUPG_004317"
     CDS             complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="ACHUPG_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFR0863638.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLK"
     gene            complement(1269..>1581)
                     /locus_tag="ACHUPG_004318"
     CDS             complement(1269..>1581)
                     /locus_tag="ACHUPG_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HFR0863639.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
BASE COUNT          482 a          400 c          406 g          293 t
ORIGIN      
        1 tttcaaaggg aggatgatga gatcgggaat aaaaacaaat acgactaata atgaatataa
       61 aaccaggacg taagggaaaa cgcctctgac tgcatcatcg aatttgagtt ttgccacccc
      121 tgaaataacg ttaagtacat taccgatagg cggtgtaatt aagccgattg agcagttaat
      181 gataaacatg acaccgaaat aaatcggatc gattcctgcc tctttaacta aaggcattaa
      241 taccggggta agaattaata ccgtcggcgt taaatccatg accatgccga caatcaggat
      301 agccaccata atgacgataa acagcagacg cggtgaatcg accaacggtt gcagtaaatc
      361 agaaaccatc atcggcagtt cagcaatggt aatcagccag gcggaaactt gtgccgaggc
      421 caccagaaac ataaccactg aggtggtttt cgccgcgcca atgagtacat gccaaagtgt
      481 ggcgaaggtc atttcacggt aaataactgt ggcgacaaac agcgcataga aagcagcaac
      541 ggccccggct tcggtagggg taaacagacc ggagcgaaac ccgccaataa tgatgactgg
      601 aagaaacagc gcccagatac cggagacaaa agagtgccag atttcctgca tcgttgcttt
      661 ttgctggcgg ggtaaattaa ggcgactggc ctgccgccac caggtaagca tcagcgttgc
      721 gcccatcatt atgccagggg caatgcccgc cataaacagc ttactgatgg ataatccgct
      781 ggaaacaccg aagataataa acggaatgga agggggaata attggcgcga taatgccgcc
      841 agaggcaatc agccccgccg cccggttgac cgggtagttg gcgctgcgca tcatcggcac
      901 cagtagagcg gcaacggcgg cggtatccgc aacggcagaa ccagaaaggc tcgccataat
      961 catagctgcc agcacgccga cgtagcccag cccgcctggt ttatgcccca ccagtttcat
     1021 cggcaggtca acaatgcgtt ttaacaagcc gcccgcattc atgatttcac ccgccagcac
     1081 aaagaacgga atcgccagga gggagaagct atcggctccg ttcaccagcg tttgtgccat
     1141 gatctggaca tcaaacatgt ccagccagaa cattaacgcc gccccgcaca acaacagtgc
     1201 ccaggcaata ggcaaaccga tagcaatacc acccaacaga cagcccagaa aaatcagcac
     1261 agccatgatt aagctccttg cggtggcgac gttagagaat tgctacgcgt gatgagttga
     1321 tataaatgac gcagttcaaa gaatgcgata acgaggctgg tgggcagaca agcggcatac
     1381 atcaggccga tgggcaaacc gaggatcggt gaataatcgc tccagtcctg aattgttttt
     1441 agcgttgcgc cccaggccag tgcgccacaa ataaataaga ttaaggaatg ggtaaccaga
     1501 gcgactcgtc gttgccatgc gggggagagt ttctccacca gaaaggtgac ctgaacgtgg
     1561 gcgttatcca taaaagccac a
//
LOCUS       DAZPAB010000398         1566 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZPAB010000398 DAZPAB010000000
VERSION     DAZPAB010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.404"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(72..148)
                     /locus_tag="ACHUPG_004319"
     tRNA            complement(72..148)
                     /locus_tag="ACHUPG_004319"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(112..114),aa:Arg,seq:tct)
     gene            complement(150..225)
                     /locus_tag="ACHUPG_004320"
     tRNA            complement(150..225)
                     /locus_tag="ACHUPG_004320"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(190..192),aa:Ile,seq:cat)
     gene            complement(402..1091)
                     /locus_tag="ACHUPG_004321"
     CDS             complement(402..1091)
                     /locus_tag="ACHUPG_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFR0863640.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
     gene            complement(1088..1447)
                     /locus_tag="ACHUPG_004322"
     CDS             complement(1088..1447)
                     /locus_tag="ACHUPG_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0863641.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGGRLGIKITELECA"
     gene            complement(1447..>1566)
                     /locus_tag="ACHUPG_004323"
     CDS             complement(1447..>1566)
                     /locus_tag="ACHUPG_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863642.1"
                     /translation="ELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
BASE COUNT          371 a          444 c          330 g          421 t
ORIGIN      
        1 gactaccgga atcgaactgg tgacctactg attacaagtc agttgctctg cctgctgagc
       61 taagtcggcg ctggcccacc accgaggact cgaacctcgg accgtcaact tagaaggttg
      121 atgctctatc cggatgagct agtggtggtt ggtggccctt gctggacttg aaccagcgac
      181 ctggcgatta tgagtcgctc gctctcacca ctgagctaaa gggccggagg cagaataata
      241 accatatgtc atcacatctg caaactcatc tgaccaccag cacgtttaac gtcctgtgcc
      301 atttttcagg cataaaaaaa ccgctcggcg gcgggttttg cttactttac catcgcgtac
      361 aaaatcggca aagtatcaga tttgcatgag atatatgcct tttaatctac ttttgcaata
      421 ctttgctttg aaaatgccgc tttttgttct gaacgtgttc tcatcacaaa caataaagcc
      481 tcactatcca gtcggtgaaa aatgtgtttc attacaaccc agtgaccagt aaatgttttg
      541 gaccagtttt tggttgttac tcccgccagt aatgccagct cctgatattc ataaccttcc
      601 ccaccaaaaa gttctgcttt taccgcctgc gccgccagcc agattaattt tttcaggcgt
      661 tcctgtgttt tctctgcaat tttcctagtg ccagaatgag ccttaaactc ttcccacgcc
      721 cagtgggtta tggtgatctg atactcccag caaatacttc cgctgtaaca ccacaacaac
      781 caggctttat agtgctcttc aagagacaga acagcccgcc gccacgatga tgtcgaaaac
      841 gcaacctgac tgaccagagg aattgacgcc cccttcgcca gtgattgttt tcccgggatt
      901 ggtggattat cccgcgttat catttttccg gtcacctcat cgcggtaccg gattttttta
      961 cgcctgtaac gccctgtatc gaacatggca ttctcctgcc aggcttcaag ctgacctttt
     1021 gtcgccccac tcaaatcagc ggtggcgata atgagctgct cacgaacaaa ctgtaaatac
     1081 tggttattca tgcacactcc agctctgtga tttttatccc caaccgacca ccaggaacag
     1141 gcagtccgcg cacaatattg gtttcatcaa actgctcgtc gtcgataagc aaccccgcat
     1201 gtgtcagtgc atccagtggt gctttcagga tattgtccag gtcacgacgc cgcttatccg
     1261 gtggctctgc aataatcttt atcgccagtc ttccggacag gcttaatttc agtcgctgct
     1321 gacggacaat aagcgccaca tcccggcgat aacgctcacc ggcttttgat acaaaatatg
     1381 tgctgccacg acgtcgccag taagtattca ccgtcggcgg ataaggtaaa ataaactcat
     1441 ggcgcatcag cgcagcacct cctgcaccag cttttcaaac tttccgactc tggtttccag
     1501 ctctgccaca cagtccacca gctcatctac tgctttctgt gcgcgatgct tcgcctgcat
     1561 cagttc
//
LOCUS       DAZPAB010000399         1557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZPAB010000399 DAZPAB010000000
VERSION     DAZPAB010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.405"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..689)
                     /locus_tag="ACHUPG_004324"
     CDS             complement(<1..689)
                     /locus_tag="ACHUPG_004324"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR0863643.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWT"
     gene            complement(686..1048)
                     /locus_tag="ACHUPG_004325"
     CDS             complement(686..1048)
                     /locus_tag="ACHUPG_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFR0863644.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            complement(1310..>1557)
                     /locus_tag="ACHUPG_004326"
     CDS             complement(1310..>1557)
                     /locus_tag="ACHUPG_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0863645.1"
                     /translation="GISNFRFHDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYA
                     HLSAGHLTEHASKIDAIISRNGTNTAQEENVVYLNAR"
BASE COUNT          441 a          359 c          326 g          431 t
ORIGIN      
        1 gtccatacgc gaagaggaaa ggttgaaaaa cttgtgatac cttccagtgc cagattccat
       61 aatttccagc cattaaattt tgagatgcca gcaacacgct ctgcgcgtac atattcaacg
      121 acatccgtcg gaccacccac ccagctcagt atgcctttca tgaaaagatt gcgctcaggc
      181 aacagtttaa tgttctccac aacctcacga gacatgagtc gaaaatctcc gacattttcc
      241 tcgatctttg gggtgcttat tttgttgtgt aatttataga accactcagc tgtcttacgt
      301 ttcagtcgtc catcagttga gcggtctgaa cgtttagcaa gcaccatgtc agcacctgcc
      361 tgccactttt caataagatg agggataacc tcaattgggt cttgcaggtc gacatcaata
      421 ggaattacag catcgccgct tgcatggtct aaccctgcaa ataaggctgg ttctttacca
      481 aagttgcgtg taaatgacag cggaacaact agcgggtctg aaacagccag cgcgttaata
      541 attgactctg tggcatcttt acttccgtca tttatgaata caatttctac ttcatatggc
      601 ttcaactctt ggaattcacg taccgtttta tagaaaacag gtatcgcttc ttcttcattg
      661 aagacaggaa cgacaagaga tattttcatt tcgcatccct aaagacaatg aactttgaat
      721 agacgaaacc gcacaccaga ctgatggcgg agaaggtgat aagagtgact attggaggaa
      781 gtgagcattt atcagctgcc caaccaacaa tcacactcaa tattcccata aatcccacgt
      841 ataacatgta gcgcatcgct gtagtcaatg ctttgaatgt gaatcttgca ttcgcgaaga
      901 agctaaagct cacagccact acgaaacctg tgaagtttgc cagagcctga ctggtatgtg
      961 cggcatagat acatacacca aaaaccaccc agtgtataag tgtgttcagc acacctatag
     1021 atgtgtactt tacaaataac tttaacattt atttaatcaa tgagctctga aaggcatgaa
     1081 gtctatcatc caagtctcaa ttgatcgata cttgctatgt ctgatgagac aaaactgaga
     1141 cacataaggc ctcacaatgg cttgcaaggc tttacatgtt ttgatgtggt gggacgtgtg
     1201 agcgcagtgt tgatgggata atcctttgaa ttacaagcgg attcttataa ttcgtaatgc
     1261 gaaggtcgta ggttcgactc ctattatcgg caccatttaa atcaataagt tacctcgcat
     1321 ttaagtaaac tacgttctcc tcttgtgccg tatttgtgcc attgcgactt ataatcgcat
     1381 cgattttgct cgcgtgctcg gtaagatgcc cggctgaaag gtgggcgtat ctttgaacca
     1441 tttcgagagt ttcccatcct cccatctctt taagcgcaag aagagagaca ccggactgaa
     1501 ccagccagct tgcccaggta tgcctcaggt catggaagcg gaagttgcta atgcctg
//
LOCUS       DAZPAB010000400         1523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZPAB010000400 DAZPAB010000000
VERSION     DAZPAB010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.406"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..656)
                     /locus_tag="ACHUPG_004327"
     CDS             complement(<1..656)
                     /locus_tag="ACHUPG_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFR0863646.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     "
     gene            complement(820..915)
                     /gene="ymiC"
                     /locus_tag="ACHUPG_004328"
     CDS             complement(820..915)
                     /gene="ymiC"
                     /locus_tag="ACHUPG_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR0863647.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(1029..1196)
                     /gene="yciX"
                     /locus_tag="ACHUPG_004329"
     CDS             complement(1029..1196)
                     /gene="yciX"
                     /locus_tag="ACHUPG_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR0863648.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(1199..1339)
                     /locus_tag="ACHUPG_004330"
     CDS             complement(1199..1339)
                     /locus_tag="ACHUPG_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR0863649.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT          414 a          361 c          352 g          396 t
ORIGIN      
        1 ttgatcatag tggtgtgcga gtcagtacca acgagtgtat ccggataagc aatccattca
       61 ccgtcctgca attcgctcca cactgctttg ccgagatatt cgaggttaac ctgatggcaa
      121 atgcctgtgc ctggcggcac gacgctaaac cgactgaacg cttgctttcc ccatttcagg
      181 aacacataac gttcgtggtt gcgctccatt tccaggcgta cgttttcttc aaatgcctca
      241 tcatcaccaa aacgatcgac ggtcaccgag tggtcaatga ccaggtcgac cggtgagagc
      301 gggttaacct ttgcagtatc gccaccgagg cgtttaaccg cttcgcgcat tgccgccaga
      361 tcaacaacgg caggtacgcc ggtaaagtcc tgcatcagca cccttgccgg gcggtaggca
      421 atttcacggt cagcatgggc atttttcagc catcccgcca gtgcgtggat atcctcttcg
      481 gtaactgagt taccatcctg ccagcgcagc aggttttcga gcaaaacttt gagtgacttg
      541 ggtagacggg tgatatcgcc cagtgattta gcagcaagcg gcaggctgta gtagtggtaa
      601 gttttatctt tggcctgcaa cgtgtcctta ctggcttctc gtagggttga cgacatagct
      661 cctccttaat gacagggttg cgaagccctg attctcttca gggtgaatat taaagataac
      721 acaaacatcg cgtaacgatt tgataacaac ccaaattgat aaaagaggag aaaccttgtg
      781 gcaaagcaaa tgacaacccc gccgcagcgg ggtcaaggat cagtgagtaa tgatccagag
      841 gagttcggcc cagaagagca tcgacagaga aaacgcgccc atccaggacc aatatttcat
      901 atttgtgttg atcataggct acacccttaa cggttattat tttgtgttga gataactcaa
      961 gctgttattg ctgcggtgta ttaaaagccc tttaattcat taaaaatggg taataagaat
     1021 gatgataatt attgtttcgg ctgatcatct tcagcaaaca gttctataaa ggcttgctgc
     1081 tgcgatgtta gttgccagtc gtctggaata acgacggaac attcccgttt cgtttctggt
     1141 tcattgtcac tatgctggca taatggtgac gactccagtt gctcctgacc gaccatagtt
     1201 atccccacac tttccaaatc agcagtgcga caaccaccca gaaaagtgca gaaccaagaa
     1261 aaaccgccag ccaggcttta cgttttaatt caggatcacg gcggggttcc tgatttccag
     1321 aaggcattgc taacctcata taatcgccat cacttatcag caagacgttg aatgattggt
     1381 gtaattaatc atacgatgag acaaatccta aagaaacttt agtcaaaaat ccagcgaatt
     1441 tacgaatcgt ctccagtgaa taattcaatc ttttgacctg aaatagatat ttgaaaactg
     1501 gaatttgcga ggcgggtaat tag
//
LOCUS       DAZPAB010000401         1483 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZPAB010000401 DAZPAB010000000
VERSION     DAZPAB010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.407"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..84)
                     /locus_tag="ACHUPG_004331"
     CDS             complement(<1..84)
                     /locus_tag="ACHUPG_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0863650.1"
                     /translation="MSRKTQRYSTEFKAEAVKTVPENQLSIS"
     gene            complement(152..634)
                     /locus_tag="ACHUPG_004332"
     CDS             complement(152..634)
                     /locus_tag="ACHUPG_004332"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0863651.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(625..927)
                     /locus_tag="ACHUPG_004333"
     CDS             complement(625..927)
                     /locus_tag="ACHUPG_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR0863652.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1199..1471
                     /locus_tag="ACHUPG_004334"
     CDS             1199..1471
                     /locus_tag="ACHUPG_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863653.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          425 a          367 c          317 g          374 t
ORIGIN      
        1 actgatcgaa agctgatttt caggaaccgt tttgacggct tcggctttaa actccgtaga
       61 gtaacgttgg gtttttctgc tcattattgg ctccttctga tgccattcta tttcaggaag
      121 gagtgtccgt taaactcagg ctacctcaga ctcagacttt ataaaacaag gtattaggtg
      181 ccacaactga ctgaacaaaa ccaaaacgct cataaaatgc tctggcgtca tcatcaattg
      241 catgtaccat aacagcctta atgccaacct gatcagccag attgacaacc cggtgaatgg
      301 catcacttag tagccattta ccgaaaccat gcccctgggt acaaacatca acagccagac
      361 gccctaacag aacaacaggg acaggattgg gcatgttatg ccgcaaactg cgaccaagct
      421 cggcatgagt gacggaacca gtagctaatg agtagtatcc cacaatacgt aaagtatcct
      481 ccaagcaaat aacgaacgtg cgagaagcat tgagcatctg gtttttcata gcccgaccac
      541 gtaaccagtt acttagcacc tcatttccac agtcaaatgg ctgaagtata tgctcatccg
      601 tcaacaatgc tggcgcggtg acatttattt ccattcaggc tttaccgcca tcagacgttc
      661 acgcccttct gcattctgca caggagcttc cagttgcgta ataaacgaat cataacgttc
      721 gttatccagc acaaataaac gttgatccag gataatgttt ttagcttcct ggtacgccag
      781 ttccatgatg aaatcggtac ggttcttatt gaccaagctc gcagcataat caataacggc
      841 tcgttcctca tcagttgccc ggatgttaat ctgatgttct cgcggtgttt tacggacgct
      901 tgcagctgta gacataaaaa cctccattgg taacaatttg ctatgcaatt aatgttatcg
      961 caaatggtaa cagaacgcta tacagaatca cttacgggaa aagcgatccc ctgataaaaa
     1021 caatggcaga gacctgaaca ttgtgacgat gatctgtgtc ggcgcacaaa tcatgtctga
     1081 ttacggccgt ttaatgtgat tttgataatg aaatgcctga accacggcag cctggcggct
     1141 gcaaatcggg gtgtggcaga atatgagctt tcagagtaac catccagaat gagttcccat
     1201 gtcacacaac ctggagcacc agaaagtcca caccagaatg gtgaaagagg ttctgaaagc
     1261 cgtcgccaga gccaataatc acccttataa gtccgtattt gcagacttta tcaccggtca
     1321 tccctcctgc acggtctgtt tctgggaaac cttccacaaa atgtacccag attcaccgta
     1381 cgaatacgtc acgttctgcc atacctgccg tcgttttgat ttatacgaaa cagaagcgga
     1441 gatgaaggcg gatgacccga agtggtggtg attttcagcc ccg
//
LOCUS       DAZPAB010000402         1472 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZPAB010000402 DAZPAB010000000
VERSION     DAZPAB010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.408"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(447..1376)
                     /gene="virB"
                     /locus_tag="ACHUPG_004335"
     CDS             complement(447..1376)
                     /gene="virB"
                     /locus_tag="ACHUPG_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HFR0863654.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
BASE COUNT          409 a          237 c          265 g          561 t
ORIGIN      
        1 aattatattt gagaaatctg ctaatatatt cttaaagctt ttttgatact gtgaacgaag
       61 tctgttaatg gtggattttt tgtttatcac catgtaaaaa aaattgagta gtaaaagcgt
      121 tgactgctaa tcctcgctgt ttttattgaa acgattaaaa atacgctgaa tattttcggc
      181 cagtcactca tattcacata aaattttgac catcatgccg catcccttag ttgcacaatt
      241 cattttcaat gatatgaata ttactaattt caccagataa ttttctcgtg gtcagcaggg
      301 tatgagtttt caccgtaatt ttaaggaaac acagtgcgtg gggattgaat aacagatagc
      361 cgacggaatt accccaccgg ctgaaagtca agttcaacga agatttttat tatctgaatt
      421 gggcagttta catcagtgtt cgatgtttat gaagacgata gatggcgaga aattatatcc
      481 cgaatagctt catctatttc tctctgaact gttttgttta ttttagataa tgtaaatatt
      541 agagttctgt ttgttttatt ctcttttctt tttatgtaag tccgtttgtc tttagaaata
      601 aataacgagt caacttttag cgaagggttt tgctttctgt ttttgctttt ctttatgatg
      661 tttaaaattt cttttttata tatgtccggg ggcaaatttg tatcaaggtc ttttatttct
      721 tcctttaata ttggtagtgt agaactaaga gattcattag ccttttcaag tcctttataa
      781 taattgaata atatcttgta gtcattaaag ttaagttctg acgcgattgg aaatagagat
      841 attatttcct gtggaacgct tgctgcctga aaggcacgag tgactttcgc gcgagacaga
      901 ttctcttttt tggctatgtc tttataggac atccctgata ctttcagaaa attaagacca
      961 ataccaagtt ctcggatgct atgctctttt gctgtttgta tatcgtttgc tagttttctg
     1021 gcatcaagag tagatatata ttcttttgaa tatagaactt caagatctgc tcctgcatat
     1081 attgcagatg ctcttctacg agtgccatcc agaatttcaa ttctaccatc aatctccctt
     1141 cctattacag ggaagaattg ttgtagcttt atagttttta tgatatcggc taatgattct
     1201 tctgttaggg attcttgatc gcgtacattc gttttttggt tgacgaaggt taaatcttgg
     1261 atccttttat gaggaatctt ggctttgata aaggaaactt tattaccaga atggagtgta
     1321 aactctttct tttggccttc ctttatactt aacaagtcgt tgcacaaatc caccatatca
     1381 caccctgttt attcatattg atttatattg acaaaagtta aatgcagtgt gagcaagatt
     1441 gtatgtaact gttgatgata gtcaacggta gt
//
LOCUS       DAZPAB010000403         1446 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZPAB010000403 DAZPAB010000000
VERSION     DAZPAB010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.409"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(1..774)
                     /locus_tag="ACHUPG_004336"
                     /pseudo
     CDS             complement(1..774)
                     /locus_tag="ACHUPG_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            782..1208
                     /gene="tnpA"
                     /locus_tag="ACHUPG_004337"
                     /pseudo
     CDS             782..1208
                     /gene="tnpA"
                     /locus_tag="ACHUPG_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          357 a          359 c          336 g          394 t
ORIGIN      
        1 gctgacggtc gttgtgactt tgtgaaggta gtccctgcgg atatttgcga tacaggaatg
       61 cagtcgctgt attttgcgtt tctgcttctg ccagttgttg ctgaatttga ccttgcggct
      121 taactgtcgc tgaagtctcg ccagcgtctt ctggtttttc tgaaaactgt ttacaggctc
      181 aaagactgtg ccatctgaca gcgaggcaag tttagccacg ccagcatcca gcccgaccat
      241 tgatgctgaa gggtgaaccg gagttgatac ttcactttct gcctgaatac tgatgtacca
      301 cttaccgcag gactggctga cagtgacatt tttcacaaca cccgtgacct gacggctgtt
      361 ccggtagcgc atccagccaa gttttggcag aaaaatacgg ctgttttcct gagcgagctt
      421 aacgccctgc ggatagcgga atgcatcatt ctgtcaccgc tttttgaatc aggggaacgc
      481 cgagcgcttc tggaaaaagt ttttgcaggc ctgctcaggg tctttcagtg actgttgcaa
      541 tggctgtgac ggagcatctt taatccattg cgtttcagtg gcatttttcc actcaaccaa
      601 tcaggaagcc attttcgcgt aagggatgtc tttgttaccg gccttatgat tctcattctg
      661 acgtgccaga gtacgactga aaacgaaacg acatgcgcct gcgaagcgcc tcatttcacg
      721 cgcctgttga ccaccgggtc ttaaccggaa tttgaatacc tgtagtcgtt tcacagggca
      781 aattatactt tggtctgtga aaaaaactga tatccgacgc ggaaggcact gtgttttcct
      841 gatgcatgct caccgggtct ttgttaccaa gtaccgacgc cagatttttg accatgacgc
      901 gacagaaaaa ctacgcactt acttttcaaa tgtatgtgct tattttgaag ctgaactggt
      961 tgaaatggat ggcgaaccag atcacgtcca tctgttaatt aactatcccc ccaaactggc
     1021 gatatccagt ctggtaaaca aacagcctca aaggcgtatc gggtaggtta ctgcgacggg
     1081 atcgaccaga tattgctgcc aggtattacc gcaaagatgt tttgtggcgt cccggctatt
     1141 ttgccagtag ctgcggaggt gcgccaatat ccgtcatccg ccaatacatt gaacaacagc
     1201 aaacacctgg tcagatgaaa aaacgagcct tatatccccg ccctgaagga cgggctttta
     1261 cggtgcactg gataagcctg gagattatct acacctgcat ggacatcatt gtactatgcc
     1321 ccgtacatgc tccatcacat cctcaacatc aaagtattag agccttgcaa accattatgc
     1381 gtctcagttt gtctcatcta tcttacgcaa aaaaaataga actaatatca agcggcgcta
     1441 tattgc
//
LOCUS       DAZPAB010000404         1432 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZPAB010000404 DAZPAB010000000
VERSION     DAZPAB010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.410"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            429..598
                     /locus_tag="ACHUPG_004338"
                     /pseudo
     CDS             429..598
                     /locus_tag="ACHUPG_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            744..>1432
                     /locus_tag="ACHUPG_004339"
     CDS             744..>1432
                     /locus_tag="ACHUPG_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0863655.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWV"
BASE COUNT          347 a          331 c          444 g          310 t
ORIGIN      
        1 ccaacacaaa ggcaactaat aaagatacag ataaagatct aaaaacagat ctaacccatc
       61 ccaaaccctt cccttccgga agggagtttc gggattttgt ggctggagtg cttgagggga
      121 gattatctgg cggtactgca gcggaatttt gtaattctgc ggtggttgcg ttgcaggctg
      181 ctggcctgga tgtctgtcgt gagtatccgg tgccagagcg tggtgacggt tgcggagggc
      241 ggattgatat cgtcgtgact gacaggaacg gtgtccggtg tgggatcgag cttgaccgaa
      301 attctccgcg acagaaatca ctgctcaaaa tcggtgctgt tgaaaccggg atatgtgtct
      361 tgcggcgcag tgatatcgca aggcacaccg agcagggaat tctggttatc ggtggggctg
      421 ttcgccagaa aaaatttgac ccgttgtcag ttgatctgcc cgactggttg tcagaaacac
      481 tctggcatga gtgggtccag ttcaggcagg cattgcgaaa accgattcga acggagctgg
      541 gcgctaacgg ggcgatacgg gaactggaaa aattccgtca gcagggtttt acacctgagc
      601 aggtgattcg acacagcatc gccaatgaat accagggtct gttcgcgccg aaaggtgttc
      661 ggcctgagac gttgctccga caggttaaca ccgtctcgtt gccggacagt gcgatcccgc
      721 caggcttcag ggggtaacag accatgaaaa atattgcgac aggaggcgtt ctggagcgta
      781 tccgcagact gaccccacca catgtaaccg ccccattcag aacggttgcg gagtggcgcg
      841 agtggcaact tgctgaaggc cagaaacgtt gcgaggagat caaccgcctg aatcgtcagt
      901 tgcgggtgga aaaaatcctg aaccgctccg gcatccagcc gttgcaccgg aagtgttcgt
      961 ttgcgaatta ccgggcgcag aacgacggtc agcgacatgc actgagtcag gcgaaatcca
     1021 tagctgacga actgatgaca ggctgtacga attttgtgtt cagcggtaag cccggcaccg
     1081 gaaaaaatca tcttgcagcc gcgattggca accggctgat ggcgaagggg cgtagcgtga
     1141 ttatcgtcac cgtgtccgat gtcatgagcg tgttgcatga gagctacgac aacggcaaat
     1201 ccggtgaaaa atttttacag gagctttgtg gtgttgacct gctggtcctg gatgaaattg
     1261 gcatgcagcg ggatacgaaa aacgagcagg tggtactgaa ccagattgtt gatcgccgga
     1321 cggcatcgtt acgcggtgtg gggatgctga caaatattaa ccatgcagcg atgaatacac
     1381 ttctcggcga gcgggtgatg gatcgcatgg tcatgaacgg cgggcgctgg gt
//
LOCUS       DAZPAB010000405         1412 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZPAB010000405 DAZPAB010000000
VERSION     DAZPAB010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.411"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..292
                     /locus_tag="ACHUPG_004340"
     CDS             <1..292
                     /locus_tag="ACHUPG_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR0863656.1"
                     /translation="AYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLFES
                     LCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            298..1395
                     /locus_tag="ACHUPG_004341"
     CDS             298..1395
                     /locus_tag="ACHUPG_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0863657.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSIRIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIESLNLGGEHPGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIALDEIIELLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNAL
                     WIELEGVEYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          437 a          320 c          346 g          309 t
ORIGIN      
        1 cgcttatccg atttttgatc gtattgaaga aatggcatgg gctcgtcact accagcagat
       61 cgttcgtgaa gaaaaagaaa cggaactggc ggacgacctg gaaaaaggtc tgcctcagca
      121 cctgtttgaa tcgctctgca tcgaccattt gcaacgccac ggtgccaata aacaggcaat
      181 cagccacgca tttgatgatg atgttgagtt tcaggagcgc atggcagaac acatccggta
      241 catggctgaa accattgccc accaccaggt tgatattgat ttggaggact aaaatctatg
      301 gcgctttttc aacgagcaat aaacacacag gcctatctaa aagcaggaat tatggggttt
      361 gctggcgacg gcaaaaccta cacagcaagc gaactggcta ttggtctggt aatgcttatg
      421 cggcaacggg gaattgaagc tggtaaccgc ccggtaatgt ttctggacac agaaacaggc
      481 tctgactggg ttaaaccccg ctttgatgcg gaaaatatcg aattatttac ggcaaaaacc
      541 cgctcgtttg ttgatctgct tgaagccatt aatgaagcgg aaagcagcgg ttccgtaatg
      601 atcatcgact ccatcagtca cttctggacc ggattatgcg acgagtatgc cagacgccga
      661 aaccgtaaac gaggactgga attttcagac tgggcgtggc tcaaacagga gtggcgacgc
      721 tttaccgatc gatttgttaa cagtcaggcg cacattatta tgtgcgggcg tgcaggatat
      781 gagtatgact ttttcgaagg tgatgatggc aaacgccagt tggcaaaaac cggtataaaa
      841 atgaaagcag aaacagaaac cggttatgaa ccctccatcc ggatccagat ggagaagcaa
      901 atgaacatag aaaccggtca ggtgtggcgc accgccagaa tactaaaaga tcgctccacc
      961 cgtattgatg gacaggtatt ttcaaaccca acatttaaaa actttcttcc tcatatcgag
     1021 tctcttaacc tgggaggtga acaccccggc atcgatacct cacgggataa cagtgaatta
     1081 tttgccaacg acggtacacc gacatggcta aaagaaaagc gtgcaaaaga aatcgcactt
     1141 gatgaaatta tcgagttgct caacaagcac catgggggca ccagtaacga cgccaaacgc
     1201 gcaaaagctg atttgctgga aaaaacattc acctcccgct catgggaaag aatcaaaggt
     1261 atggactggc cgacaatcaa agctggtcgt aatgcactat ggattgaact ggaaggcgtt
     1321 gaatatgcat tcccggatcc acaaacacaa aacgaaacgc aacaagcagg atacgatgag
     1381 aatattccgg tgtaaaacca agtgcatttt gg
//
LOCUS       DAZPAB010000406         1330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZPAB010000406 DAZPAB010000000
VERSION     DAZPAB010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.414"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(41..100)
                     /locus_tag="ACHUPG_004342"
     CDS             complement(41..100)
                     /locus_tag="ACHUPG_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0863658.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..1265)
                     /locus_tag="ACHUPG_004343"
                     /pseudo
     CDS             complement(339..1265)
                     /locus_tag="ACHUPG_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
BASE COUNT          266 a          354 c          321 g          389 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttatgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 tgttcggtaa tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc
     1321 ttgtcatgca
//
LOCUS       DAZPAB010000407         1328 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZPAB010000407 DAZPAB010000000
VERSION     DAZPAB010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.415"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..217
                     /locus_tag="ACHUPG_004344"
     CDS             <1..217
                     /locus_tag="ACHUPG_004344"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863659.1"
                     /translation="QQILPSQVVRLAKIVGDVVVSYSRLMSSGINPLHCEYVKHQKKM
                     NQIYEKSKYTYQGRKHDITLIPLWQIF"
     gene            283..>1328
                     /locus_tag="ACHUPG_004345"
     CDS             283..>1328
                     /locus_tag="ACHUPG_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0863660.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          433 a          297 c          214 g          384 t
ORIGIN      
        1 gcaacaaata ttaccaagtc aagtagtcag gctggctaaa atagttggag atgttgtagt
       61 atcttactca agactaatgt cttctggtat aaatcctctg cattgtgaat atgtaaaaca
      121 tcaaaaaaag atgaaccaaa tatacgaaaa aagcaaatat acttaccagg gtagaaagca
      181 tgatataaca ctcattccct tatggcaaat attctgaaac accctcctca ttaggagaga
      241 tacattaaat acaatttatt cacaatctaa ttccatatcc atatgcgaga aattaatatg
      301 ctcagaaaca tttcatcctg tttatttcca catatcagca caattacatc ccccaaccat
      361 tatttgtccg aatgggatga ttgggagaaa caggggttac cggaagaaca gcgtactgag
      421 gcggtaagaa gacttcgtgc atgtcttacc tctaaggggc ataaactgga cctgcgagcc
      481 ttggcgcttt cctcgctacc tgtactccct gcttgcatta aaaagcttga tgtgagctgt
      541 aataaattaa ccatccttac tgatttacct gaaaatatta aagaacttat tgcaagagat
      601 aatttcttaa cacatatatc tgcattacca cattatctaa taactttgga tgtgtccgaa
      661 aatcaattag agaatctgcc gttattacca gacaccatca aatcactaag cgcagagtat
      721 aataggttat ccacactgcc ttcattaccc ttgaatttaa aaaaacttga ggttaggaac
      781 aacgaactgc aaactcttcc atctctgcct tctaatctta agatacttaa ggttgcgcac
      841 aaccatctta ctgaactgcc ccctttacct aggagactgc aacttctttt tgcatatagc
      901 aatagattaa gcaacttacc aaacatccaa gaaaatatta tcatgagaag atttttttat
      961 tttgaaaaca accaaataac tacaatcccg acaaatcttt ttcgtttaga tcctcatata
     1021 actattgaga ttgcaaataa ccccttatca gatcaaactc tgctattctt aatacagcaa
     1081 acttcggttc caaattttaa cgggcctcag tttcgtattt ccctgtcaga ccaaaacaga
     1141 ctgtttttac gccagatgtt gccgcaaaat ttacattcgc gccatatcag agtcatcact
     1201 gaaggggggc agaactttca gatcccccct cttcccgaaa ctgtggcagc ctggtttcct
     1261 gaagcagatc gtcgggaggt ttctacacaa tggacttctt tttccaccga ggagaattcc
     1321 cgggcatt
//
LOCUS       DAZPAB010000408         1326 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZPAB010000408 DAZPAB010000000
VERSION     DAZPAB010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.416"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(207..362)
                     /gene="hokD"
                     /locus_tag="ACHUPG_004346"
     CDS             complement(207..362)
                     /gene="hokD"
                     /locus_tag="ACHUPG_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR0863661.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(590..1255)
                     /locus_tag="ACHUPG_004347"
     CDS             complement(590..1255)
                     /locus_tag="ACHUPG_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR0863662.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          330 a          339 c          311 g          346 t
ORIGIN      
        1 ccatccctct tctccctgcg cgctacttaa gtgagtcgat tctatctgcg aaggtgcgcg
       61 aatcaaattc accggaaata aacaacaaaa aacccgccga aacgctgaga gatcacagcg
      121 atgggcgggt taagtgcggg tgcgttgagg atgcctgaca catcagaggt gggcggggat
      181 gggatcagct ccccacccgg tcactcttac ttcctggatt cgtagtctac gaagacagcg
      241 acctccgtct ggccggttcg gattcgtacc tcacagaggt ctttcctcgt taccagtgcc
      301 gtcactatga cggttaaaca gatgacgatc agggcgatta acatcgcctt ttgctgcttc
      361 atagcctgct tctccttgcc tttcggcatg taagaggcta acctacatgt gcaaagcatg
      421 aaattggcct cagattaatg ttaagcgtct tgccggacgc gtaatgttaa ctggggcttt
      481 tctctatctg ccttttggtg ttcatgcctg aagcagatag cctcaagcac ccgcagtcat
      541 tctacttacc tcgccaatat gaaatcaatc agaaaggtgc cccataaaat cactccttct
      601 cttctttacc gtagtggagt tgaccaattt tgataagagg gcgtccctga gatttgcggt
      661 gtagattggt atcgcgcaga gaatacacac agccacaata ttcctgctga tagaattttt
      721 cgcgcttgct gatttcaatc atacgggacg agccgccctg cttgcgccag ttataatccc
      781 agtacaccat gcccggataa tgcgcgacgg ctcgccgccc acagtcgtta acctgctgca
      841 tatttttcca gcgtgaaatg cccagtgaac tgctgatcac actgaaacca ttttcagcgg
      901 cgtacagcgc tgtccgctca aaacgcatgt caaaacacat ggtgcaacgg atccctcgtt
      961 cgggctccca ttccattcct ttggctcgtt caaaccagtt gtctgtgtcg taatcagcat
     1021 caataaacgg cacgccgtgt tgttcagcaa agcgaatatt ctcatcctta cgaattaaat
     1081 actctttctg aggatgaatg ttcgggttgt agaaaaagat ggtgtagtcg atttccgagg
     1141 cctgaagcgc ctccatcacc tcaccggaac atggagcaca gcaagagtgc agtagtagtt
     1201 tgtttgcccc gtttgggagc tccaatttgg gccgtttgaa atcagcaact gtcataaata
     1261 tgtttattga ggtcataaaa atatcaaaga gtgtagcatt agaacggggc tatcggaaac
     1321 agatgt
//
LOCUS       DAZPAB010000409         1303 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZPAB010000409 DAZPAB010000000
VERSION     DAZPAB010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.417"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            31..1303
                     /locus_tag="ACHUPG_004348"
                     /pseudo
     CDS             31..1303
                     /locus_tag="ACHUPG_004348"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          366 a          234 c          332 g          371 t
ORIGIN      
        1 atgttttatc gactgtcatt aattttgata atggccttac ttgcgggtca gttaagtgcc
       61 caggagtggt catttgattc ttcacagttg gaaggaaacg ttagtgcgga cactgtggca
      121 atgtttaatc agggagagca actccccgga aattatcgtg tggaaattta tcttaacggt
      181 gaaaaagttg atgttgggga gtttcccttt catcgtccgg aatcccctga agaaaaagag
      241 cttgttccat gtttaaccgt tgatgacctt atccactatg gcattaaaat tgataaaagc
      301 agcagtgata ctgacaataa aaagaatcag tgttttaaat ggaactctat tgaaggtttg
      361 aaagtaaatt atgattttga tagccagcgc gtacagataa ccgttccaca actgtatctg
      421 caagataaaa aaagtagttt agcaccagtg tcgttatgga atgagggtgt agctgcattc
      481 cgcatggtgt atcagacaaa tattgatatt tccaaacaaa atgataatca atctaccacg
      541 cgtaattctc gttatggcag gttcactcca gggtttaatc ttggtgcctg gaggtttcgt
      601 agttcagtga catggagcaa ggaacttggc cagtcagaaa gatggcaaag gggttacatg
      661 tggtttgagc gaggcataaa tgcgattaaa agccgattaa cgcttggaga atcttatacg
      721 tcgtctgagg tgtttgacag catacctttt cgcggcggga tgttagcgac cgacgatgcg
      781 atgactccgc cggaggacag ctactacaca cctgtggtgc atggaatagc acaaagtgag
      841 gcgcaggtta ttattaaaca aaatgggcaa attattttta cccgttctgt gccgccaggg
      901 ccttttgcgc tggataattt accaacgctg gccgttggcg gggagcttga tgttacagtg
      961 agagagagta atggcgagga gcagtatttt tccgtgccat ttcaaacgcc ggccatcgct
     1021 ctgcatgaag gatatttcaa atatagtgtg atggggggga atataaagaa gaaggtataa
     1081 aaagctcccc tgtctccgtg ggcgaaatca cggcgatgta cgggcttcct tatggcgtca
     1141 cggtgtatgg agggattcaa aacgccactc attttaatgc aatatctacg ggtattggca
     1201 tttctctggg gctgttaggg agtttatcga cagatattac gcgttcgatt gcgaatttat
     1261 attatgggaa taaatatcgt attcgttata gtaaatccat tag
//
LOCUS       DAZPAB010000410         1295 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZPAB010000410 DAZPAB010000000
VERSION     DAZPAB010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.418"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            142..1140
                     /gene="yiaK"
                     /locus_tag="ACHUPG_004349"
     CDS             142..1140
                     /gene="yiaK"
                     /locus_tag="ACHUPG_004349"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFR0863663.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            1152..>1295
                     /locus_tag="ACHUPG_004350"
     CDS             1152..>1295
                     /locus_tag="ACHUPG_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFR0863664.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQ"
BASE COUNT          325 a          335 c          324 g          311 t
ORIGIN      
        1 tttataacca cttgattttt atgtcttttg aaattcatca atcagattgc tttgttaaaa
       61 agtgatcgat atatttgaaa tctagttccg catattgaaa ttttaagcca aaaaagcgat
      121 caaaaaacaa ggaagcctag gatgaaagtg acatttgagc agttaaaagc agcctttaat
      181 cgggtcttaa tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc agagatgttc
      241 gcccgcacca ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt
      301 caacaactgg aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc
      361 ggcgcaattg aacagtggga tgcccagcgt tcgatcggca acctgacggc gaaaaagatg
      421 atggatcgcg ccattgaact ggctgccgat cacggtattg gtctggtggc acttcgtaat
      481 gccaaccact ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa aggctatatt
      541 ggcatttgct ggaccaactc catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc
      601 atcggcacta acccgctgat cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg
      661 tcgatgtcga tgttctctta cggcatgtta gaagttaacc gcctggcagg ccgtcagctc
      721 ccggtcgatg gtggctttga tgatgagggc aatttgacca aagaacctgg cgttatcgag
      781 aagaatcgcc gcattttgcc gatgggctac tggaaaggtt ctggcatgtc gattgtgctg
      841 gatatgatcg ctactctcct ttccgacggc gcatcggttg ccgaagtcac ccaggacaac
      901 agcgacgaat acggcatttc acaaattttt attgccattg aagtggacaa gcttatcgac
      961 ggtcccaccc gcgatgccaa gctgcaacgt atcatggatt acgttactac cgctgaacgc
     1021 gctgacgaaa accaggccat ccgcttaccc ggccacgaat ttactaccct gctggccgaa
     1081 aaccgccgta acggcatcac tgttgatgac agcgtgtggg ccaaaatcca ggcgttatga
     1141 ggagataagt catgattttt ggacatatcg cgcagcccaa tccgtgccgt ttgcccgccg
     1201 ccattgaaaa ggcgctcgat tttctgcgcg ctaccaactt caacgtcctg gagccgggcg
     1261 ttgtcgaaat cgacggcaag aatatttatg cgcaa
//
LOCUS       DAZPAB010000411         1293 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZPAB010000411 DAZPAB010000000
VERSION     DAZPAB010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.420"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            223..1221
                     /locus_tag="ACHUPG_004351"
                     /pseudo
     CDS             223..1221
                     /locus_tag="ACHUPG_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
BASE COUNT          306 a          278 c          284 g          425 t
ORIGIN      
        1 aattaatctt aggataaata tttatttatc atggcctttt gaccgctaaa aaatggcact
       61 cggcacagta aaccccgcta tagttatgct atgcatttgt ctttaatgat atctcgtcgt
      121 tatattaaca ggctgaaatt atcattaatg cacatttcaa tattagcagg gatacctgag
      181 gagtttaaag tgaacatata tatcgggtag cttttcaaat taatcccttt gattataggc
      241 ttaatttgca tcgcgttagg tggctttgtg ctggaaagtt cagggcaacg tcacgggtca
      301 tgtgctgatt tctctggcgg ccatatgcct ggcattattc actaccgcat ttattatcat
      361 ttcgcaactc acgcgcggcg ttaatacgtt ttacaataca ttgttcccca ttattggcta
      421 tgcggggtca attatcacca tgatatgggg ttgggcactg ttagcaggta atgatgtgat
      481 ggcagacgag tttgttgccg gccatgttat ttttggcgtt ggtatgattg ccgcatgtgt
      541 atcgacggtg gcagcgtcgt ccggtcactt tctgctcatt cccaaaaatg cagcggggag
      601 caagagcgac ggaacaccgg tacaggctta ttcttcatta atcggtaatt gcctcattgc
      661 cgttcccgtt ttactcaccc tgctcggttt catttggtct attacgctgt tacatagtgc
      721 cgacataact ccgcattatg tcgccttggg ttaaccgcaa tctgtgcctg tctaattggc
      781 ctagtggcca caatcgtcca tcaaacacgt aatacgttct catccaaaga gcactggctg
      841 tggtgttatt gggttatttt tctcggctca gtcacggtgc tgcaggggat atacgtctta
      901 gtcagttccg atgcaagcgc ccgactggct cccggcatta ttctcatttg cctcggaatg
      961 atctgttaca gcatattctc aaaagtctgg ctactggcac tggtctggag acgtacctgt
     1021 tcgttagcca acagaatacc gatgataccg gtatttacct gtctgttttg cttgtttttg
     1081 tcatcattcc tcgcggaaat cgcacaaact gagctggact actttattcc atctcgggtt
     1141 ctggttggat taggcgcagt atgttttaca ttgttctcta ttgtttcaat attagaggcc
     1201 ggttctgcga aaaaaaatta aaatttacat aatcccagcg ttattcatat cttaacgctg
     1261 gttttttacg aacttagtga aataatgtag aat
//
LOCUS       DAZPAB010000412         1284 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZPAB010000412 DAZPAB010000000
VERSION     DAZPAB010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.421"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            522..806
                     /locus_tag="ACHUPG_004352"
     CDS             522..806
                     /locus_tag="ACHUPG_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFR0863665.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            806..1081
                     /locus_tag="ACHUPG_004353"
     CDS             806..1081
                     /locus_tag="ACHUPG_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR0863666.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          339 a          321 c          324 g          300 t
ORIGIN      
        1 acagaataag tttacgcgta aatcgccctg aatccagaag agcattaagt atcacataaa
       61 caggttccat accagtcctg accgcatcag aacgccagta aataaaatct cgtagtccac
      121 cggcatcaga gacaagagta atcgtcgtta ccggtatttc gactggtttg tactggtgtg
      181 agcggacaaa actactgtac gtctctcata cacgctcgta agcggccctc acggcccgct
      241 aacgcggaga tacgccccga ctgcgggtaa acccttgtcg ggaccactcc gaccgcgcac
      301 agaggcattt tcatcgctga caggccgcat ggcctcgcag agccggaggg ggactggtga
      361 tattcggacg accgtaccgg cttgcgccgg gcttcggcgg ttttacacct gtgctacatg
      421 taaccaccgt atgccgcctt atatgcgctg actcgcatta tttgccttgt tccgtctgaa
      481 agtaatcact atgattaaat atgattaaca actaatcggg tatgcaaatg aaaaacaata
      541 ccgcacaagc aacaaaagta attaccgcgc atgtgccatt acctatggct gataaagtcg
      601 accagatggc cgccagactg gaacgctccc ggggctgggt tatcaaacag gcgctttctg
      661 catggcttgc ccaagaggag gagcgtaatc gcctgacgct ggaagccctg gacgatgtga
      721 catccggaca ggttatcgac catcaggctg tacagtcctg ggcggacagc ctcagtactg
      781 accatctgtt accggtgcca cgctgatgga actgaagtgg accagtaagg cactttctga
      841 tttggcgcgg ttatatgatt ttctggtgct ggccagtaaa cctgctgccg ccagaacggt
      901 gcagtccctg acacaggcgc cggtcattct gttaactcat ccacgtatgg gagaacagtt
      961 gtttcagttt gaacccaggg aggtcagacg gatttttact ggcgagtacg aaatccgtta
     1021 cgaacttact ggccagacta tttatgtatt gcgcctgtgg cacacacgag agaacaggta
     1081 gattgggtgt ggaggcaact ttaacatagc ctgcaggtat taattctgga gcattgttat
     1141 caggccagat attttgcatt ccattaaata atcttgtagg agataaaata aattacgatg
     1201 aaataacgaa aattaccgct cgtgactggc ggcaatatcg cgctccgggc tggcaaataa
     1261 cccatcagaa acgttattgc caga
//
LOCUS       DAZPAB010000413         1277 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZPAB010000413 DAZPAB010000000
VERSION     DAZPAB010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.422"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="ACHUPG_004354"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="ACHUPG_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HFR0863667.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          359 a          229 c          285 g          404 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttgtt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actatctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtc
//
LOCUS       DAZPAB010000414         1261 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZPAB010000414 DAZPAB010000000
VERSION     DAZPAB010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.423"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..888
                     /gene="yhfZ"
                     /locus_tag="ACHUPG_004355"
     CDS             <1..888
                     /gene="yhfZ"
                     /locus_tag="ACHUPG_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR0863668.1"
                     /translation="KKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVGLTQAAL
                     KTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEGLASGLK
                     AQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALELGPHTY
                     VGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESLQRIVKG
                     DVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLLRAVVDK
                     HALLAHQQRVVSGEQEPSY"
     gene            905..>1261
                     /locus_tag="ACHUPG_004356"
     CDS             905..>1261
                     /locus_tag="ACHUPG_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR0863669.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEE"
BASE COUNT          326 a          269 c          368 g          298 t
ORIGIN      
        1 aaaaaagaag gcgtcgtcat cacgacactg gcccgttatt tgttgggtga aaaatgcggt
       61 aatcgactga aaaccataga tgagctggca actgaatgcc gttcatcggt tggcctgacg
      121 caggccgcgt tgaaaacgct ggaatcaagc ggagcgatac ggattgaacg ccgtgggcgc
      181 aatggcagtt atcttgtcga gatggataac aaagcattgc tgagtcatgt ggatatcaac
      241 aacgtggtat gtgcgatgcc tttgccctat acccgcttgt acgaaggttt agcgagcgga
      301 ttgaaagccc agtttgatgg cattcctttt tactatgcgc atatgcgcgg cgcggatatt
      361 cgcgtggagt gtttacttaa cggtgtctat gacatggcag tagtttcccg cctggcggcg
      421 gaaagttatc tcagccaaaa caatttgtgc atcgccctcg agcttggccc gcatacgtat
      481 gttggtgaac atcaactgat atgccgcaaa ggtgagtctg gaaatgttaa gcgagtaggt
      541 ctcgatagtc gttcggcgga tcagaaaatc atgaccgatg tcttttttgg cgatagtgat
      601 gtggaacgag tcgatctctc ttatcacgag agtttacaac gcattgttaa aggcgatgtc
      661 gatgcggtga tctggaacgt ggtggcggaa aacgaactga ccatgctggg attagaggcg
      721 acgccgctaa cagacgatcc gcgattttta caggccaccg aagccgtggt cctgacgcga
      781 gtcgatgatt acccaatgca acaattgctg cgtgctgttg tagataaaca cgccctgctt
      841 gcccatcaac aacgggtggt gagtggggaa caggaaccca gttattaaac gaaaggcatc
      901 tgatatggaa accagactca acctgctttg cgaggcaggc gttattgata aggacgtctg
      961 caaaggcatg atgcaggtcg tcaacgtact ggaaaaagag tgccatctgc cggtgcgcag
     1021 tgagcaagga acgatggcga tgacacatat ggcgagtgca ctgatgcgca gtcgccgtgg
     1081 tgaagaaata gagccgctgg ataacgagtt gctggcggaa ctggcgcaat ccagccactg
     1141 gcaagccgtt gtgcaattgc atcaggagtt gttgaaggaa ttcgctctgg aagttaaccc
     1201 gtgtgaagaa ggctattttc tggccaatct ttatggatta tggatggctg ctaacgaaga
     1261 g
//
LOCUS       DAZPAB010000415         1228 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZPAB010000415 DAZPAB010000000
VERSION     DAZPAB010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.424"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            113..448
                     /locus_tag="ACHUPG_004357"
     CDS             113..448
                     /locus_tag="ACHUPG_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR0863670.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(729..837)
                     /locus_tag="ACHUPG_004358"
                     /pseudo
     CDS             complement(729..837)
                     /locus_tag="ACHUPG_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(896..>1228)
                     /locus_tag="ACHUPG_004359"
     CDS             complement(896..>1228)
                     /locus_tag="ACHUPG_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863671.1"
                     /translation="GLMVFSALSIYHSYGVGRVNIDEASGCFILRQRIDIVNAARAKA
                     FSRLTVLFCTPDRLSGRDVIILNSDAIQRVCDEFMVANSELFALVQEYNRIARTCGMD
                     ELRITHLG"
BASE COUNT          413 a          306 c          200 g          309 t
ORIGIN      
        1 ttaaataaac aataaacaac atcagaaaaa taattctgtt ctatggttta attcaaaaat
       61 atcatttata cttttcagaa catcaccagc aaggcataaa caaggaaact aaatgaagtg
      121 gattgtgatt gatacagtta tccagccatc atgcggaata tctttttcag tcatatggag
      181 taaaataaaa ttaataatct ggtatcaatc ggatgctttc ttacctcctg aaagtatatt
      241 tacactgact cacacaggca tcatgctcaa taacaaagtg ctacctgtaa ccatttacaa
      301 cgtagtacca ttcaataaaa cattctggaa tttaatcaaa aacagccagg aatgccctac
      361 aaatacagat aacgtattga atgaatgctt taataaccgt tgcactctgc aaatatgccc
      421 ttatggacta aaacaacaaa gtccataagg agtttactca catctgacaa aatcaatata
      481 aacagcccct ccggagaggg gctggagagt ggcgctatgt gccattccat ggtgccgggt
      541 gcctcccggt gaattcagta ccagcacctg aatccgcgat tatcccatat acctactcgc
      601 tgattgcccc tccgcacagg gggattcacc atgccagttt cttttaacaa actccccgca
      661 aaccagacaa cagtcaaccg cctgaattgt gaagtattta aaaatttctc ccgctaactg
      721 atacccggct aacagtctgg cgttttcttt ttcagcaacg ggaaagcaac aaccaccaca
      781 cccgccacca gcacaccgtc agccggcact gacattatcc ggctgctgca atgccattaa
      841 caaaaacagt aagcaatcac tttttaccgt aacaggtgat aatccagata tgtatctacc
      901 ccagatgagt aatccgaagt tcatccatac cacaggtcct ggctattctg ttgtactcct
      961 gaacaagagc aaataattct gaattagcaa ccatgaactc atcgcaaacc ctctgtatag
     1021 catcactatt cagaataata acgtctcttc ccgaaagacg atcaggagta cagaacaaaa
     1081 ctgtcaaacg gctgaaggcc tttgctcgtg ctgcattgac tatatcaata cgctgcctaa
     1141 ggatgaaaca ccccgacgcc tcatcaatat tcactctacc cacaccatat gaatgataaa
     1201 tacttaatgc tgaaaaaacc attagacc
//
LOCUS       DAZPAB010000416         1227 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZPAB010000416 DAZPAB010000000
VERSION     DAZPAB010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.425"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(646..>1227)
                     /locus_tag="ACHUPG_004360"
     CDS             complement(646..>1227)
                     /locus_tag="ACHUPG_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR0863672.1"
                     /translation="LSFMLSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLEL
                     TRDRFFGKSKIGFIVFSITAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTII
                     TTVGLIIFNIFIAKIICGNDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPN
                     PICAIIYTCICVYVSDKIGHWLSRTVGGPFLIK"
BASE COUNT          386 a          220 c          222 g          399 t
ORIGIN      
        1 tcatacataa acaatatgac tcaatactgt cctaaatagc tgcgcagaaa agtgctccac
       61 tttcgctcag cccttgctgg gcgaagcatc aggaaggcgt tctcagctag gttgtgttat
      121 atctggcttg agttttgttg cccatacgca acacattacc catctgtaaa cgacaagtgt
      181 ggcagaatta ggtgcagcta tttagactat atcgacgcag taacgataat agatatcgaa
      241 actgtactag atattagcta cttatggtta ccggggggag tcttttgagc actggtgtta
      301 tcaattggct tcaaataatt cgccaataaa ttatattgcg atagctcatc accttccagg
      361 ttgtttatag ccagtttatc agcgtcacca cctactgttt atgcttttat ttctttgatt
      421 aataagtata tgatatttta attcatctgg tgtatttctt tctcatgtgt tggtcgactg
      481 aatatctgtt cagtgaaggg gtaattttat gtttgcactt cgaacattcc cttcagttgc
      541 taggaaagaa aaccgtaaca tttgcgcatt aattttaaca gtataatctg agcataaata
      601 tgcgctatat atctataaaa aggcgcaatt aattgcgcct ttttatcatt ttatcaaaaa
      661 tggcccaccg actgtcctcg atagccaatg gccaatttta tctgatacat atacacaaat
      721 acaggtatat atgattgcgc agataggatt tggaatagtc acgctgaagc ctataattgc
      781 taaagccata gggataaata atttggtgat atactccatt ccacatatgt taagagtatt
      841 tcttccagct cttacaataa agtcatttcc acaaattatt tttgctatga aaatattaaa
      901 gattatcaag cctaccgtgg tgataatagt attaatagct tgaatataac tacttgatat
      961 tatgtctgca ttgaatattg agataatctt aaaaagtaaa ggtttctggt taagtagttg
     1021 atacgcactc attatagcgg taattgagaa gacaatgaaa ccaattttag attttccaaa
     1081 aaacctgtca cgtgttaatt ctaaaaacat acatctaccg agaggcaata accaccagta
     1141 tgccatagcg gagtctatgt tccaaaacca ttgaggatct agtaatgggt tatgattgag
     1201 cactgtttgt gacaacatga acgataa
//
LOCUS       DAZPAB010000417         1227 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZPAB010000417 DAZPAB010000000
VERSION     DAZPAB010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.426"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(261..>1227)
                     /locus_tag="ACHUPG_004361"
     CDS             complement(261..>1227)
                     /locus_tag="ACHUPG_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR0863673.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          363 a          273 c          265 g          326 t
ORIGIN      
        1 tgtgttgtat tagcgactga aatctgcctt ttaaaaagga ggagggtact gagacaaaga
       61 gtcattgctt ctccgataaa atgtcagaga agcatgatta cgtggttatt gtctgttgat
      121 gacttgttga ccgagccgtc atcattctca atgcattagt taaatcattt gtgattttta
      181 aggattatcc tgtctatgat tttctactta gaaggatatg gcactaacta tgcatcttat
      241 agtttactgc tggcatctat ttaaaatagc aattgagtca aatagcccat caagatagcc
      301 atacctaata taaccgtcag gaatgcagct atcatcggca ttctgaacat tgatttaagt
      361 aatatcactt ccgtcaggct tgcgccggca ctgccgatga ttaatgccat taatgctccc
      421 agacccattc cttttgtcat cagaacagat gccagaggga taaccgcctc agcccggata
      481 tacagcggaa taccgacaac ggcgcttaat gggatggcga agggattatc tgctcctgca
      541 tgagcggcaa tccacgctga gggaataaag ccataaataa aagatcctat taatactcct
      601 aacaaaagat atggcagtac atctttaaac tgttgcaacg catctttggt ggctagtttt
      661 atgggattaa tacttactac agctttggta ctgcaacagg taactacggg gttctcaatg
      721 gctttagccg ctggactaca acaactcact tttatgggcg tatatgtcgt tcccggcgaa
      781 gtttttgctg gagcacaaca atttgctgat gagcttttac tggcaatgat atgacgttca
      841 aaccccagag aatccaggat aatactggca agaacggaga cgccggctgc gataatcgcg
      901 tacaacaagg tgactttcca accaaaggtc atccacatta gtccgacgat aatgggatta
      961 agtaatggtg aaacaaataa aaaagtgaga gtcggaccaa acccggcttt cgctgacaac
     1021 aatccacgta acatgggtat tgtcgagcaa ctacagaacg gggtaacggc tcccaacaga
     1081 gcagccagga gatagcctct cccttttctg gcccccatca tctgctggat tttatggtct
     1141 ggcacctttt gtcttatcag gctgacaccg gcactaatca ctataaacag caacgaaagc
     1201 tcgacggcga gaaacacaaa catctct
//
LOCUS       DAZPAB010000418         1224 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZPAB010000418 DAZPAB010000000
VERSION     DAZPAB010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.427"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..965)
                     /locus_tag="ACHUPG_004362"
     CDS             complement(<1..965)
                     /locus_tag="ACHUPG_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863674.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQE"
BASE COUNT          356 a          187 c          223 g          458 t
ORIGIN      
        1 aactcttgct tatattttaa tatgtcatca aatgaaagat tcactacctt tgcctctaag
       61 aaatcaatta ttctcgaagc aatttccgct ttcgcgcgta aagagttgtc attaatagtt
      121 aacaatgtag aaaacaaacc attgatttgt ttgtcaagat gttgttcata tttatgtagt
      181 ttggggatgg gattttcctt gtgtgaaaaa atagtgttaa ataaatttgc gtcagtgaaa
      241 tttatattaa gtagatcttc aaaataaaat tttgataagg ttaggactag cttggacata
      301 tcttgccctg tcatgttgaa atcaataaag tcgcatccag caagggcttc catcctattt
      361 tcatttttac tgtgctcaat atacagcttc gccattttta agtacaagct ttgaatgtct
      421 cttatatcag ttggtaagtc agctcgttgc ccagcaaaat ttttattatc ggaagaaacg
      481 gtttggacac tgaatctttc gggtattcct tcttctacga gaattttagt tatgattgga
      541 ttgttgggtt ctggattaac gacaacaatg atttgacata cagaatcctt atccttttcg
      601 tatctaatgt tatgaatctg attaagtatt gattgtgtct gttgcgtaag tggtgtcgaa
      661 cttatcgttt caatgttatt aataagactt acaagttgaa ccacaacttt tgagtagtct
      721 gcatttgtat atgtggtgtt cgtgtggcat gcattaattt gttggagttg ttgttgcgct
      781 aaatcaaatt gttcagggtt gtatccttct ccagagaatg tggaattaat gggttgttgt
      841 tcttcccaat tatcacaagg ccacttcggt gtgtctggcg ttttaacatt atcggtattt
      901 gttgtgcagt tataaatatc gccgtgatat gtatttatta tacctggggt aaagtcaatt
      961 gacataacat tccttattca tcatctgttt tatgctaatg tttttaaatt ggataattag
     1021 aacaggaatg gattttcttc tacacacaat aaatataatg cgttatatct tcctgatata
     1081 attaccttta aattcccttt catttccacc cggtaatatt tgtgtataaa aaaagggcag
     1141 tgatagtatc gtaaaggaaa acgttaacaa ctatcaaaaa acgctcattt taaaaagtta
     1201 ttatgttctc cgatagtgtg tgaa
//
LOCUS       DAZPAB010000419         1222 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZPAB010000419 DAZPAB010000000
VERSION     DAZPAB010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.428"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(68..592)
                     /gene="sat2"
                     /locus_tag="ACHUPG_004363"
     CDS             complement(68..592)
                     /gene="sat2"
                     /locus_tag="ACHUPG_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR0863675.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(687..1160)
                     /gene="dfrA1"
                     /locus_tag="ACHUPG_004364"
     CDS             complement(687..1160)
                     /gene="dfrA1"
                     /locus_tag="ACHUPG_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR0863676.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
BASE COUNT          303 a          282 c          273 g          364 t
ORIGIN      
        1 cttccctcat gatgtttaac tcctgaatta agccgcgccg cgaagcggtg tcggcttgaa
       61 tgaattgtta ggcgtcatcc tgtgctcccg agaaccagta ccagtacatc gctgtttcgt
      121 tcgagacttg aggtctagtt ttatacgtga acaggtcaat gccgccgaga gtaaagccac
      181 attttgcgta caaattgcag gcaggtacat tgttcgtttg tgtctctaat cgtatgccaa
      241 ggagctgtct gcttagtgcc cactttttcg caaattcgat gagactgtgc gcgactcctt
      301 tgcctcggtg cgtgtgcgac acaacaatgt gttcgataga ggctagatcg ttccatgttg
      361 agttgagttc aatcttcccg acaagctctt ggtcgatgaa tgcgccatag caagcagagt
      421 cttcatcaga gtcatcatcc gagatgtaat ccttccggta ggggctcaca cttctggtag
      481 atagttcaaa gccttggtcg gataggtgca catcgaacac ttcacgaaca atgaaatggt
      541 tctcagcatc caatgtttcc gccacctgct cagggatcac cgaaatcttc atatgacgcc
      601 taacgcctgg cacagcggat cgcaaacctg gcgcggcttt tggtacaaaa ggcgtgacag
      661 gtttgcgaat ccgttgctgc cacttgttaa cccttttgcc agatttggta actataattt
      721 atgttagagg cgaagtcttg ggtaaaaact ggcctaaaat tgctggggat ttcaggaaag
      781 taaacatcac cttccggctc gatgtctatt gtagatatat gtagtgtatc tacttgatcg
      841 atcaggcttt tgtatatctc cccaccacct gaaacaatga catgatccgt tattttcttt
      901 aggttggtta aagcatcttt aattgatgga aagatcaata cgttctcatt gtcagatgta
      961 aaacttgaac gtgttacgac cgcatacttt cggttgggta atgctcccat tgattcaaaa
     1021 gtcttgcgtc caaccaacag ccattggtta taggtaatag ctttaaacag gagctgttca
     1081 cctttggcac tccatggaat atcagggcca ttcccgataa ctccattctt cgatatagct
     1141 accattagtg atagtttcac aattcttcct cagaggttaa cattttatta gtaagcatgc
     1201 tcgttttgtc acccgctgat gc
//
LOCUS       DAZPAB010000420         1218 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZPAB010000420 DAZPAB010000000
VERSION     DAZPAB010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.429"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..>1218)
                     /gene="ospC4"
                     /locus_tag="ACHUPG_004365"
     CDS             complement(<1..>1218)
                     /gene="ospC4"
                     /locus_tag="ACHUPG_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HFR0863677.1"
                     /translation="NKSITKNILRVTNSSSSGISEKHLDHCANTVKNFLRKSIAAQSY
                     SKMFSQGTSFKSLNLSLEAPSGARSSFRSLEHLDKVSRHYISEIIQKVHPLSSDERHL
                     LSIIINSNFNFRHQSNSNLSNNILNIKSFDKIQSENIQTHKNTYSEDIKEISNHDFVF
                     FGVEISNHQEKLPLNKTHHTVDFGANAYIIDHDSPYGYMTLTDHFDNAIPPVFYHEHQ
                     SFFLDNFKEVVDEVSRYVHGNQGKTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREF
                     CYNKNIDPVSLDRIINFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEINN
                     KVTDKKMAHQALAYSLGNKKADIALYLLSKFNFTKQDVAEMEKMKNNRYCNLYDVEYL
                     LSKDGANYKVLEY"
BASE COUNT          387 a          193 c          196 g          442 t
ORIGIN      
        1 aatattctag cactttatag ttggcaccat ctttactaag taagtattca acatcataca
       61 gattacaata tctattgttt ttcattttct ccatctctgc gacatcttgt tttgtaaaat
      121 taaatttaga tagtaaataa agtgctatat cagctttttt attgcctaaa gaataagcaa
      181 gggcctgatg agccattttt ttatcagtca ctttattgtt aatctcttca taattagatg
      241 ctttaatcgc atcctccaaa gatatgtctc tgagttttat ttttttgaag ttatctgtac
      301 ttagcattct tggtatatga tactcaagct gaaacacaaa gtttataatt ctatccagac
      361 taacaggatc aatatttttg ttataacaaa actctctgaa tccttgatct ttactctttc
      421 ttataaaatc aataagatgc aacccaattc caagcctcat atctttagtg ttaaatattg
      481 gaacatcagt tttcccctga ttaccatgca catacctgct aacttcatcc acaacctctt
      541 taaagttatc taagaaaaat gattggtgct catggtaaaa aacaggtgga atagcattat
      601 caaagtgatc ggttaatgtc atatatccat atggagagtc atgatcaatg atatacgcat
      661 tggcgccaaa atctacagta tgatgtgttt tatttagtgg gagtttttct tgatgattgg
      721 aaatttcgac cccaaaaaaa acaaaatcgt ggttagagat ttctttaata tcctcagagt
      781 atgtattctt atgtgtttgt atattttcag actgaatttt atcaaaggat ttaatattaa
      841 gaatattatt tgacagatta gagttacttt gatgtctgaa gttaaaatta gaattaataa
      901 taatagaaag taagtgacgt tcgtctgaag aaagaggatg aactttttgt attatctcag
      961 agatatagtg cctggacacc ttatcgagat gctcaagtga ccgaaatgat gaccgtgctc
     1021 cagatggcgc ttcaagagaa agatttagtg atttgaaact ggtcccttga gaaaacattt
     1081 tgctataact ttgcgctgct atagacttcc ttaaaaagtt tttcacagta ttagcacagt
     1141 gatctaaatg tttttcactt atgccagaag aagatgagtt tgttacgcgt aatatatttt
     1201 ttgtaatgct tttgttgc
//
LOCUS       DAZPAB010000421         1194 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZPAB010000421 DAZPAB010000000
VERSION     DAZPAB010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.430"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..672
                     /gene="fimC"
                     /locus_tag="ACHUPG_004366"
     CDS             <1..672
                     /gene="fimC"
                     /locus_tag="ACHUPG_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFR0863678.1"
                     /translation="LMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTAVWIKNSH
                     TNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAADRESLFWM
                     NVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSRSGNYINV
                     HNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDYGSVTPVR
                     EVNLN"
     gene            703..>1194
                     /locus_tag="ACHUPG_004367"
     CDS             703..>1194
                     /locus_tag="ACHUPG_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR0863679.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
BASE COUNT          335 a          262 c          255 g          342 t
ORIGIN      
        1 ttgatgctca tgatatttta tttaatcatt tcggccagcg cccatgctgc cggagggatc
       61 gcattaggtg ccacgcgtat tatttatccc gctgatgcta aacagactgc ggtatggatt
      121 aaaaatagcc ataccaatga gctctttctg gtcaattcgt ggattgaaaa cagcagcggt
      181 gtaaaagaaa agtcattcat cattacaccg ccactatttg ttagtgaacc caaaagcgaa
      241 aatactttgc gtattattta caccggtcca ccgctggcgg cagatcgtga gtctctgttc
      301 tggatgaatg ttaagacgat cccttcggta gataaaaatg cattgaacgg caggaatgtt
      361 ttgcaactgg cgattttatc gcgcatgaaa ttatttctcc gtccaattca attacaagaa
      421 ttacccgcag aagcgccgga cacactcaag ttttcgcgat ccggtaacta tatcaatgtt
      481 cataatccat caccttttta tgtcaccctc gttaacctgc aagtgggcag ccaaaagttg
      541 gggaatgcta tggcggcacc cagagttagt tcacaaattc ccttaccctc aggagtgcag
      601 ggaaagctga aatttcagac cgttaatgat tatggttcag taaccccggt cagagaagtg
      661 aacttaaact aaccgaatca tctgacaata tcagagctaa ttatgaaaat acccactact
      721 acggatattc cgcagaggta tacctggtgt ctggccggaa tttgttattc atctcttgcc
      781 attttaccct cctttttaag ctatgcggaa agttatttca acccggcatt tttattagag
      841 aatggcacat ccgttgctga tttatcgcgc tttgagagag gtaatcatca acctgcgggc
      901 gtgtatcggg tggatctctg gcgtaatgat gagttcattg gttcacagga tatcgtattt
      961 gaatcgacaa cagaaaatac aggtgataaa tcaggtgggt taatgccctg ttttaaccag
     1021 gtactccttg aacgaattgg ccttaatagc agtgcatttc ccgagttagc ccagcagcaa
     1081 aacaataaat gcatcaattt actgaaagct gtacctgatg ccacaattaa ctttgatttt
     1141 gcagcgatgc gcctgaacat cactattcct cagatagcgt tgttgagtag cgct
//
LOCUS       DAZPAB010000422         1190 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZPAB010000422 DAZPAB010000000
VERSION     DAZPAB010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.431"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            29..778
                     /gene="phoC"
                     /locus_tag="ACHUPG_004368"
     CDS             29..778
                     /gene="phoC"
                     /locus_tag="ACHUPG_004368"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase PhoC"
                     /protein_id="HFR0863680.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(833..937)
                     /locus_tag="ACHUPG_004369"
                     /pseudo
     CDS             complement(833..937)
                     /locus_tag="ACHUPG_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(948..>1190)
                     /locus_tag="ACHUPG_004370"
     CDS             complement(948..>1190)
                     /locus_tag="ACHUPG_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0863681.1"
                     /translation="ELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGR
                     LKEYRRIATRYDKTARNYLAIVKLGCIRLFYQRLRN"
BASE COUNT          317 a          267 c          259 g          347 t
ORIGIN      
        1 atatgtatcc tctataagga gatgttatat gaaaagacag ctttttactc ttagtattgt
       61 cggggtattt tctttaaata cctttgcatc attccctccg ggaaatgatg tgacaacaaa
      121 acctgacctt tactacctga caaacgataa tgctattgac agtctggcat tattaccgcc
      181 tccaccacaa atcggaagta ttgccttcct gaatgaccag gccatgtatg aaaaggggcg
      241 tttattgcga aatactgaaa gagggaagct ggcagctgag gatgccaatc tcagtagtgg
      301 tggcgtggca aatgtatttt ctgctgcttt tggttctccg ataacggcta aagattcacc
      361 agaattgcat aaattactga caaatatgat tgaggatgcc ggagacctgg caacgcgttc
      421 agcaaaagaa tattatatgc gtattcgacc ttttgctttt tatggtgttt ctacgtgtaa
      481 tacaaaagaa caggatacat tatccagaaa tggctcttat ccatcaggtc atacatcgat
      541 tggttgggca acagcacttg tattgtccga aataaatccg gcacgccagg ataccattct
      601 gaaacggggt tatgaactgg gggacagcag ggttatttgc ggttatcact ggcaaagtga
      661 tgttgacgca gcacgtattg ttggctctgc tattgtagca acactgcact caaatcctgt
      721 gttccaggca caattacaaa aagcgaaaga tgaattcgct aacaatcaga aaaaataatg
      781 cttttagcca gtacgcctta taaccccgac cttcagggct aatgccagtc agttaagcaa
      841 ctgactggct ctttttcagg gctgtggggt atttccagtg cctctccttt accactctcg
      901 gaaaggccct ttcccttctt gtcggtaatt tcactaggct gtgtccctta attacgtaag
      961 cgttgataaa agagtcggat gcagcccagt ttcactatcg ccaggtaatt tctcgccgtt
     1021 ttgtcgtaac gcgtggcgat gcggcgatat tctttcagcc gcccaaagca ccgttcaacg
     1081 acattacgat tgcagtacgc atcacgatca agctgtgcac gtccatccga tgccattttc
     1141 tcatttgact ttcgggggat tactgccttt ataccgttat ttttcagctc
//
LOCUS       DAZPAB010000423         1183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZPAB010000423 DAZPAB010000000
VERSION     DAZPAB010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.432"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..721
                     /gene="phoN2"
                     /locus_tag="ACHUPG_004371"
     CDS             <1..721
                     /gene="phoN2"
                     /locus_tag="ACHUPG_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HFR0863682.1"
                     /translation="LFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPPPPAENSV
                     VFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTPILYQLLT
                     QVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHASFGWAVA
                     LILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLHNTPEFTK
                     SLSEAKKEFEELNTPTNELTP"
     gene            794..916
                     /locus_tag="ACHUPG_004372"
     CDS             794..916
                     /locus_tag="ACHUPG_004372"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863683.1"
                     /translation="MGTPRFTPEFKGLLERLGHIPPAEAEKAYYAAIGNDDLAT"
     gene            complement(1081..1167)
                     /locus_tag="ACHUPG_004373"
     CDS             complement(1081..1167)
                     /locus_tag="ACHUPG_004373"
                     /inference="COORDINATES: protein motif:HMM:NF017898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863684.1"
                     /translation="MLDNAMKSKDSKMIDFFIKKWSGIRQTI"
BASE COUNT          346 a          261 c          249 g          327 t
ORIGIN      
        1 tcttttttgt attgctacaa atatgatttt tatcccctca gcaaatgctc tgaaggcaga
       61 aggttttctc actcaacaaa cttcaccaga cagtttgtca atacttccgc cgcctccggc
      121 agagaattca gtagtatttc aggctgacaa agctcattat gaattcggcc gctcgctccg
      181 ggatgctaat cgtgtacgtc tcgctagcga agatgcatac tacgagaatt ttggtcttgc
      241 attttcagat gcttatggca tggatatttc aagggaaaat accccaatct tatatcagtt
      301 gttaacacaa gtactacagg atagccatga ttacgccgtg cgtaacgcca aagaatatta
      361 taaaagagtt cgtccattcg ttatttataa agacgcaacc tgtacacctg ataaagatga
      421 gaaaatggct atcactggct cttatccctc tggtcatgca tcctttggtt gggcagtagc
      481 actgatactt gcggagatta atcctcaacg taaagcggaa atacttcgac gtggatatga
      541 gtttggagaa agtcgggtca tctgcggtgc gcattggcaa agcgatgtag aggctgggcg
      601 tttaatggga gcatcggttg ttgcagtact tcataataca cctgaattta ccaaaagcct
      661 tagcgaagcc aaaaaagagt ttgaagaatt aaatactcct accaatgaac tgaccccata
      721 aagctggaca gcctgtatca ggctatggag ggcccataga caaatctacc ctatatgagc
      781 ataggaggag tctatgggca caccacgttt tacccctgaa tttaagggat tactggaaag
      841 gctgggacat atccctccgg cagaagcaga aaaagcttat tatgctgcca tcggaaacga
      901 tgatctggca acctgagttc acagataaaa cattctctag gaaactcggg gcggttccgt
      961 tcaccacatg caatgtggtg ttgcagggga acggtctgca ccatccccta tgtcgatcaa
     1021 tataacagaa atgacaactt cagattcagg gcacaaccta aatatatttt aggtcacctc
     1081 tcaaatcgtt tgcctgatac cgctccattt tttaataaaa aaatcaatca tttttgaatc
     1141 tttgcttttc attgcattgt caagcatagt gtccccacta ttt
//
LOCUS       DAZPAB010000424         1172 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZPAB010000424 DAZPAB010000000
VERSION     DAZPAB010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.433"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..507)
                     /gene="mxiJ"
                     /locus_tag="ACHUPG_004374"
     CDS             complement(<1..507)
                     /gene="mxiJ"
                     /locus_tag="ACHUPG_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HFR0863685.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRF"
     gene            complement(513..806)
                     /gene="mxiI"
                     /locus_tag="ACHUPG_004375"
     CDS             complement(513..806)
                     /gene="mxiI"
                     /locus_tag="ACHUPG_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HFR0863686.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            complement(819..1070)
                     /gene="mxiH"
                     /locus_tag="ACHUPG_004376"
     CDS             complement(819..1070)
                     /gene="mxiH"
                     /locus_tag="ACHUPG_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HFR0863687.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            complement(1078..>1172)
                     /locus_tag="ACHUPG_004377"
     CDS             complement(1078..>1172)
                     /locus_tag="ACHUPG_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFR0863688.1"
                     /translation="DDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
BASE COUNT          361 a          232 c          170 g          409 t
ORIGIN      
        1 aaatcgctta atattactaa ctaatagttc agactctttc ggtgagtcat atatagcgat
       61 tactgatata tgcatcggtt ttgaagatat atttttttct tcaagatcat agctaacatg
      121 tatttttgcc gaaataacac caccaataga aactaaagac tgttccagcc gttgctcaat
      181 agcactatat aaacgggcct tttcagctct tggagaagac actaatgaat ctgtaggaaa
      241 catttgtgag atatcaactc tctccggatt tggcaaatcg tacatgcgca tcaaatcaac
      301 tgccgatgca aatgtcccct tttcgacttg taccgagatc ccctgtttac ctccatcaac
      361 ttttctagca gtaatattat ggcgttctag cacagatatt atttcatttg cctgtctttg
      421 agataaatta gaaattaact cttcacgttg ctcacatcca atcaacatca gcaacaagaa
      481 taaaataaaa cctttatacc taatcatata aattaagact ttaataaagt ttcaacagcc
      541 gaaactgttt ttcttgctaa cgtgccagct aatgatactc ctattgaata atttgagagc
      601 gtcgtctgta acttggccaa agattctggg tttaatgatt ctggattgct taccatctcc
      661 attatttgtg atacttgaat atctgtatcc atcttaatat cactatattt agccgacacg
      721 acgtcttcca gacttgatat ctcttgagat tgaaaatcac tggctttgat aatatcaacc
      781 tgattgactg gataaatgta attcatgaat gctccctatt atctgaagtt ttgaataatt
      841 gcagcatcaa catccttaat cactttcact gtattggatt gcgcgttcct atataatgta
      901 tattcagata atttactttg gtattcagcc agcaactgtg gattcgaagg atttttagct
      961 aatttatcta gtgccaatgt tagttcacct tgtaatgttt gagttccatc atcaaaagtt
     1021 tcagataatg aactcaatgt ccaatcatca ttcggtactg taacactcat tttatcctca
     1081 cttattttta tctaaaaaaa accagtgttt atcattcaac attacataac tgtctttgct
     1141 gttaagatat gatttacctt taaaatcatc at
//
LOCUS       DAZPAB010000425         1170 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZPAB010000425 DAZPAB010000000
VERSION     DAZPAB010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.434"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..167
                     /locus_tag="ACHUPG_004378"
     CDS             <1..167
                     /locus_tag="ACHUPG_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFR0863689.1"
                     /translation="AIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRM
                     KERRKEWGTL"
     gene            152..790
                     /locus_tag="ACHUPG_004379"
     CDS             152..790
                     /locus_tag="ACHUPG_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFR0863690.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1018..>1170
                     /locus_tag="ACHUPG_004380"
     CDS             1018..>1170
                     /locus_tag="ACHUPG_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863691.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNT"
BASE COUNT          333 a          229 c          311 g          297 t
ORIGIN      
        1 ccgctattgc cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga
       61 atgatttctg gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt
      121 acctgcagcg tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact
      181 tggcaacatg attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc
      241 tctgaatcat ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga
      301 ctgtgtgttg tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat
      361 ggcactaggt gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc
      421 ggtggtggtg tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt
      481 actggggcgg gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat
      541 taatccggag agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc
      601 cagggggaaa caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg
      661 ctggacgaat gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct
      721 gaaacagatt agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac
      781 ggttcgttga tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa
      841 aaatgatacc gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta
      901 ggctttgata acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg
      961 cagatttaca aaatctgatt gattctaaag atgtataagt aaaaatcaag ctttgatatg
     1021 aaaaaacaga tatttattaa taataaacct cctgtagtcc cttactcagg gacgcacgcg
     1081 aaaatattta aatacattga aatccctctg ccgtttttct actttatcta tacatcaggc
     1141 gaaccattcc acatatctgt ccaaaatact
//
LOCUS       DAZPAB010000426         1168 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZPAB010000426 DAZPAB010000000
VERSION     DAZPAB010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.435"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..1103)
                     /locus_tag="ACHUPG_004381"
     CDS             complement(<1..1103)
                     /locus_tag="ACHUPG_004381"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR0863692.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICT"
BASE COUNT          283 a          306 c          302 g          277 t
ORIGIN      
        1 gtacagatga actcatacgg ccccagtttg gcgatctcat ccgccagata ggcagcgacc
       61 tggtaggaag cattctgtac tttggtatag ccttcacgac caaggcgcag gaattcatag
      121 tactgtgcaa tcacctgacc cgccgggcgg gagaagttga tggcaaaagt accaatctga
      181 ccaccgaggt agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc gtcacgccag
      241 ataacccagc cgcagcccag cggagccaga ccgaatttat ggcctgaagc actgatcgat
      301 ttcacacgcg gcaggcggaa gtcccagacg atatccgggg cgacgaacgg tgccagaaag
      361 ccaccgctgg cggcgtcgat gtgcatgtcg atgtcgatac cggtgtcggc ctggaattta
      421 tccagtgcat cgtgcagcgg ttgcgggaac tcatagttac cggtgtaggt cacgccgaaa
      481 gtcggcacca cgccgatggt attttcgtcg caggcttcaa tcatgcgttt cgggtccata
      541 aacaactgac cggggcgcat agggatctca cgcagctcca catcccagta gcgggcgaat
      601 ttatgccagc agatttgcac cggaccgcac accaggtttg gtttatccgt tggtttgcct
      661 gcagcttcca tacgcttgcg ccaacgccat ttcatcgcca tcccgccgag catacaggcc
      721 tcggaagaac caatggtgtt ggtgccaacg gcctgaccat ttttcggcgc aggcgcatgc
      781 cacagatcgg caaccatgtt tacgcaacgc aggtcgatgg ctgcggattg cggatattct
      841 tctttgtcga tccagttttt gttaatggat aaatccatca acttgtggac gttttcgtcg
      901 tcccaggtct ggcagaaagt ggccaggttc tgacgagcgt tgccatcaag atataattca
      961 tcattgataa tctgaaatgc gacatcgtcg cgcatttcgt gcagcggaaa acgttttgat
     1021 tctgcgatag tggaaataga cttcgcacca aaacgtgaat cgagtagttc cgaccttaaa
     1081 tccgttactt gcttcttatc cattttaaac tccttaaaat gattggatcg cattaaaaaa
     1141 gtaggattta tcgataaagt aagcaagt
//
LOCUS       DAZPAB010000427         1159 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZPAB010000427 DAZPAB010000000
VERSION     DAZPAB010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.436"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            115..>1159
                     /gene="cadB"
                     /locus_tag="ACHUPG_004382"
     CDS             115..>1159
                     /gene="cadB"
                     /locus_tag="ACHUPG_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR0863693.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKAS"
BASE COUNT          246 a          255 c          298 g          360 t
ORIGIN      
        1 tttaggatta atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat
       61 agttatacat catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt
      121 tctgccaaga agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg
      181 agcggtattg cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt
      241 tggattatct ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca
      301 aaaaacccgc aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt
      361 tttcagacag gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt
      421 accgctgtat cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt
      481 atcgcctgta ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg
      541 gtaagccgtt taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct
      601 attgttggct ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat
      661 accactgatg gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt
      721 gttgaatccg cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg
      781 gcaaccatgc tgggtactgg tttagcaggt attgtttata tcgctgcgac tcaggtgctt
      841 tccggtatgt atccgtcttc tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct
      901 tcaactatcc tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc
      961 ctgacttctc tgggctcctg gatgatgttg gtaggccagg caggtgtacg tgccgctaac
     1021 gacggtaact tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt
     1081 ctgctgctgg ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct
     1141 gccggtggta aagcatctg
//
LOCUS       DAZPAB010000428         1143 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZPAB010000428 DAZPAB010000000
VERSION     DAZPAB010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.437"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..217
                     /locus_tag="ACHUPG_004383"
     CDS             <1..217
                     /locus_tag="ACHUPG_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFR0863694.1"
                     /translation="KKAENRIKMPKLNSDSFYNIIVAIPPYNEQQAIFDKINSIEAVC
                     NGLISYIGIYHKTQLHLADALTDAAIN"
     gene            438..779
                     /gene="symE"
                     /locus_tag="ACHUPG_004384"
     CDS             438..779
                     /gene="symE"
                     /locus_tag="ACHUPG_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFR0863695.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(826..>1143)
                     /locus_tag="ACHUPG_004385"
     CDS             complement(826..>1143)
                     /locus_tag="ACHUPG_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HFR0863696.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT          336 a          282 c          221 g          304 t
ORIGIN      
        1 caaaaaagca gagaacagaa taaaaatgcc aaaacttaat tctgacagtt tttacaatat
       61 tattgttgcg attcccccct ataatgaaca acaagcaata tttgataaga taaattctat
      121 tgaagcagta tgcaatggtc ttatttctta tattgggatt tatcataaaa cccaactgca
      181 tcttgcagac gcactcactg acgcagcgat aaactaattc cctcgccctc acaaaaccaa
      241 taaagcggct aaatagccgc tttattcaca tcaccaaaat ttatatttca cgctaatttt
      301 catcttattt tcattaattt ttagctggac tttccctgta tttactgatg atgtatccag
      361 gtatttagca cggtgcggat gtgcgccaac acactcgcac cgctaatcac aatcactatt
      421 tatagagaat agtaatcatg actgacacgc attctattgc acaaccgtta gaagcagaag
      481 tctccccggc aaataatcgt caattaaccg tgagttatgc gagtcgttac ccggattaca
      541 gccgtattcc cgccatcacc ctgaaaggtc agtggctgga agccgccggt tttgccaccg
      601 gcacggcggt agatgtcaaa gtgatggaag gctgtattgt cctcaccgcc caacccgccg
      661 ccgccgaaga gagcgaactg atgcagtcac tacgcaaggt gtgtaagctg tcggcgcgta
      721 aacaaaggca ggtacaggaa tttattgggg taattacagg taaacagaaa gtcgcctgat
      781 acattgaccc ggtagcgcca gtgctaccgg gtattattcg ccgcgttata ccaacgcgct
      841 ctccatctgt ttttcaaatg cctgacgaat taatcccata atgtaaggaa gttgtgcgag
      901 gtcgctgaaa ccaatttcta catcgccatt accccagcgc ccaacatttg tcacatcttt
      961 ggcaatacct ttcggatcga ccagttcatg aaactgcata ttaagcgaca atcgcagtcg
     1021 gcttttttgc ggcacaacat caacaaaatt cgtttccgct ttaaacgcaa tatatagctt
     1081 caatacttcc tgcgtgatcc ctgcgtccag gcgcataact tcatcgcgaa gatgatcgaa
     1141 taa
//
LOCUS       DAZPAB010000429         1140 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZPAB010000429 DAZPAB010000000
VERSION     DAZPAB010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.438"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..730
                     /locus_tag="ACHUPG_004386"
     CDS             <1..730
                     /locus_tag="ACHUPG_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFR0863697.1"
                     /translation="TDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMVN
                     KPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITFT
                     GICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASGV
                     HNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDRP
                     LPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(732..958)
                     /locus_tag="ACHUPG_004387"
                     /pseudo
     CDS             complement(732..958)
                     /locus_tag="ACHUPG_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          298 a          283 c          247 g          312 t
ORIGIN      
        1 aaccgacaga atccatttga aagacgcagc gcgaatgctg gaagtttctg taatgactat
       61 tcgtcgcgat ctccatcagg aagatgaacc tctgccactg accctactgg gtggctatat
      121 tgtaatggtg aataaacccg cgccatccat gccagtaatc catgacgttc caaaaaatca
      181 tcgtgatgac ttacctattg caattctggc tgccggaatg gttaatgaaa atgatctgat
      241 cttctttgat aatggccagg agataccact cgttataagc atgatcccgg atgcaatcac
      301 cttcaccggc atctgttact cacatcgcgt ctttgttgcg ttgaatgaaa agcctaatgt
      361 aacagcaata ctttgtggtg gtacgtatcg tgccagaagt gatgcttttt acgatgccag
      421 taactcttcg ccattagact ctctcaatcc gcgaaaaata tttatttccg ccagcggtgt
      481 gcataatcac tttggcgtca gctggtttaa ccctgaagat cttgccacta agcgtaaagt
      541 gatgaaccgt ggactacgga aaattttgct cgcccgccac gcgttgttcg atgaagtggc
      601 ctctgccagc ctcgcaccga tctctgcatt tgacgttctg attagcgatc gtccgttacc
      661 ggcagattat gttacgcact gccagaatgg ttctgtaaag atcattacac ctgattcaga
      721 agacgaatga cttactgaaa aaacaccaca atcttgttaa acatcgtcgg gttggactga
      781 ttacgttgca ctttcaccac atattccagc ttatctattt ggcttatcac ctactccaga
      841 cgctggtcat ccttgaccag tagccagata tggcttttgt cgcagtcctg aatcggcagg
      901 cagagaatgt cttcaacgtt aagagcgcga cgggcaaaag cccacaaagg tgagtcatta
      961 cacccggatg gtaaacaggc acttccttgt tgtagaagcc gttatattgt ccataatgtc
     1021 tgggttttta atgcatactg ggtaacaacc ggacgaatct tcgaatatca ctggcgctgc
     1081 cagccggttt gaattcatct cacaaccctg catagagcga atctcctgcc tgcacgtcac
//
LOCUS       DAZPAB010000430         1130 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZPAB010000430 DAZPAB010000000
VERSION     DAZPAB010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.439"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..128
                     /locus_tag="ACHUPG_004388"
     CDS             <1..128
                     /locus_tag="ACHUPG_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863698.1"
                     /translation="DLASMGQLVKLPTRRGRAFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(167..970)
                     /gene="dkgB"
                     /locus_tag="ACHUPG_004389"
     CDS             complement(167..970)
                     /gene="dkgB"
                     /locus_tag="ACHUPG_004389"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR0863699.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          302 a          256 c          264 g          308 t
ORIGIN      
        1 gcgatcttgc aagtatggga caacttgtga aattaccgac gagaagggga agggccttcc
       61 cgagagtggt aaaggagagg ccctggaaat accccacagc cccgaaaaag agccagtcag
      121 ttgcttaact gactggcatt actcccggag gagctgtcag agaggcttaa tcccattcag
      181 gagccagacc ttccgggcta accaggcggt cgttgcaatc cagtgcggcg atcgcttttt
      241 tatcttcggc atcaagctgt aaattttgtg ccttaagatt actttccagg tttttacgtt
      301 tagtagaaga aggaattact gagtaacctt cccccatagc ccacgccaga atcacttgtg
      361 ccggaatcgc attgtgttta gctgcaatac gagcaataac ctcatctttc agtgccttac
      421 cataagccag cgtcatatag gaagtgatat gaataccgtg ctgtttagcc caggcaacca
      481 ctttacggtt ttgcagataa ggagagagtt caatctggtt ggtagcgatg ttttcagcgc
      541 caacagcagc aatcactttt tccatcaatg ggatcgtgaa gttggaaata ccgatctcac
      601 gcgtcagccc ttgttttttg gcttccagca gcgcctgcat aaactcttca acagagactt
      661 catcgtttgg tgacggccag tggattagcg tcagatcaac ataatcggta cgcaattttt
      721 gcaggctctc tttcagactc gggatcaatt tgtctttgct gagattttca atccagattt
      781 tagtggtgat gtagagttca tgacgtggca cgccactttc tgcaatcgcc tgacctactg
      841 cggcttcgtt atcatagatt tgtgcggtat caattgcgcg ataaccaagt tcaagcgccg
      901 ttttcacaga tgaaataaca acgtcgtctt tcagacggaa agtacctaaa ccaaatgcag
      961 ggatagccat aatatgcctc ttttaatgct attttcgtta actgaggtga ttatgggctt
     1021 cagactgttg cgggaaaaga gcgtaaaacg cagaggattt ttgcgattct agcaataata
     1081 gatatccaga cgcaaaaaaa catcgagcgt taactcgagg cttcttaata
//
LOCUS       DAZPAB010000431         1126 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZPAB010000431 DAZPAB010000000
VERSION     DAZPAB010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.440"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            7..657
                     /locus_tag="ACHUPG_004390"
     CDS             7..657
                     /locus_tag="ACHUPG_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863700.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            629..1063
                     /locus_tag="ACHUPG_004391"
     CDS             629..1063
                     /locus_tag="ACHUPG_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0863701.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
BASE COUNT          316 a          229 c          289 g          292 t
ORIGIN      
        1 taatccatgc atcgtataga cacgaaaacc gcgaagaagg ataagttcgg cgcgggtaag
       61 aacggtttta cccgtggtaa cccccagact ggcacgcctg ccaccgatct ggatgatgac
      121 tactttgaca tgttgcagga ggagctttgc agcgtggtgg aggcatccgg tgccagcctg
      181 gagaaggggc ggcacgatca gttgcttacc gcacttcgcg cgctgctgtt aagccgcaag
      241 aatccgtttg gcgatatcaa atcggatggc acggtgaaaa cggctctcga aaaccttggt
      301 ttgggagaag cagctaaaag gaatgtaggt acaggggcga atcagatacc tgatatgagc
      361 ctgttcgcgt caattaatac cgtaacggct gctgcgcaaa aatttccgtc tggattaatt
      421 ttacagtgcg gtcagttgaa tggtgccccg aatgtatctt caacatacgg gatgaggttc
      481 ccgatgacgt tctcaagagt aattgctgtc gtagttacat tgaacgttac tggcgcggct
      541 gggcagccga ctgtatcggc gacaagtgtc cagaacactg gatttaatat tactgtgtcg
      601 cccggttcag gatacggttc atctgctgat gcgtattaca ttgcaatggg atattaacaa
      661 aatgtcatat ttttattctg catcgacaaa cggattttat tcgactgaat ttcacggcac
      721 caatattcct gatgatgcag tggaaatctc ggaatcagag tggaaaacac tgattaatgc
      781 acagagtgta acaaaaatga ttacctgtgg tgagaacggt catcctgtca ttgttgaccg
      841 tccttctcca acaccagaac aattagcctt aataaatgat gaaaagaaat ctgcactgat
      901 agcagaggca acgaatgtaa tagctccgct tcaggatgcg gttgatttag gtatggcaac
      961 agatgatgaa acgaaactgt tactggcatg gaaaaaatat cgggtgctgc ttatgcgtgt
     1021 taatgtagta aaacccgagt ggcctatgca tccaaataaa tagacttgtt aatctaggat
     1081 aattgttgga ataatactcg tttttcagta ggttcaggta gcctgg
//
LOCUS       DAZPAB010000432         1125 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZPAB010000432 DAZPAB010000000
VERSION     DAZPAB010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.441"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            59..556
                     /locus_tag="ACHUPG_004392"
                     /pseudo
     CDS             59..556
                     /locus_tag="ACHUPG_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            597..788
                     /locus_tag="ACHUPG_004393"
                     /pseudo
     CDS             597..788
                     /locus_tag="ACHUPG_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(882..>1125)
                     /locus_tag="ACHUPG_004394"
     CDS             complement(882..>1125)
                     /locus_tag="ACHUPG_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFR0863702.1"
                     /translation="IKLLTKYLFFESQGIPDKVDIVSRLKTYGYSISGVETDDGYKAL
                     VRAFQLHFRQKNYDGIMDAETAAILYALLEKYFPGK"
BASE COUNT          299 a          280 c          300 g          246 t
ORIGIN      
        1 gcgccagccc cgtcccgccc gatagagcgc ggcatagcgg gccccggatt acttgcgtgc
       61 gacggcagaa gaagccctga agcgaatcag tgaactgtac gccatcgagg atgaaatacg
      121 gggattacca gagtcagagt gtcttgcagt caggcagcag cgaagcaaag cgttactgac
      181 gtcgctgcat gaatggatgg tggagaagaa tggcacgctg tcgaaaaaat ccagactggg
      241 cgaagcattc agctatgtac tgaatcagtg ggacgccctc tgttattaca gtgatgacgg
      301 tctggcggag gcggacaata acacagcgga aagagcgctt cgtgcagtct gtctcggaaa
      361 gaaaaattac gtgttcttcg gtagcgatca cggcggcgag cgtggtgcac tgctgtacgg
      421 gctgatcggc acctgccgtc tgaacggtat cgatccggaa gcgtatctgc gccatattct
      481 gagcgtactg ccggaatggc cttccaaccg agttgacgac ctcctgccat ggaaggtggt
      541 gctaccatca ggataatcgt caatacggtg ttcggtgaac gtttacttgg agcatcatgc
      601 ttcatgaaga aaaactggca cgtcatcaac gtaaacaggc gatgtatacc cgaatggcag
      661 ccttcccggc ggtgaaaatg ttcgaagagt atgacttcac gttcgccacc ggagcaccgc
      721 agaagcagct ccagtcgtta cgctcactca gcttcataga gcgcaatgaa aacatagtct
      781 tactggggac atctgacatc acaaatcccc gggtggggat ttgtgtataa gagacagccc
      841 atggaggaag gagatatatc gcagctctga cgactcactc cttatttacc cgggaaatac
      901 ttctccagca atgcatacag gatggcagcg gtttcagcat ccattatgcc gtcataattt
      961 ttctgccgga aatgaagctg aaaagcgcgg acaagcgcct tatatccgtc atccgtctcc
     1021 acaccagaaa tactgtatcc ataggtcttc agccgtgaca cgatatcaac cttatccgga
     1081 atgccctgcg attcaaaaaa taaatattta gtcagtaatt ttatg
//
LOCUS       DAZPAB010000433         1124 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZPAB010000433 DAZPAB010000000
VERSION     DAZPAB010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.442"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..835
                     /gene="spa40"
                     /locus_tag="ACHUPG_004395"
                     /gene_synonym="spaS"
     CDS             <1..835
                     /gene="spa40"
                     /locus_tag="ACHUPG_004395"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HFR0863703.1"
                     /translation="EGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKFVLATKAI
                     KIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIFSQVFSSV
                     DGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKREYIEQEGH
                     FETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPFISLIETN
                     QCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVWLEQVENT
                     H"
     gene            857..1090
                     /locus_tag="ACHUPG_004396"
     CDS             857..1090
                     /locus_tag="ACHUPG_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator YmgB/AriR"
                     /protein_id="HFR0863704.1"
                     /translation="MGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKDIIN
                     VLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT          358 a          152 c          205 g          409 t
ORIGIN      
        1 tgagggtaaa tacttttttg cagtggttat tgtctttttt aagataattg gcttcccact
       61 ttttttctgt gttctttcgg ctgtgttgcc aacattggtt caaacaaagt ttgttcttgc
      121 gactaaagct atcaagattg atttttcagt attaaaccct gttaaagggt taaaaaaaat
      181 atttagtata aagacaataa aagaattttt caaaagcatt ctgcttctta ttattctagc
      241 attaacaacc tatttctttt ggattaatga ccgaaaaata attttttctc aggtgttttc
      301 tagtgttgat ggcttatatc ttatttgggg gaggctgttt aaggatataa tattattttt
      361 cttggcattt tctattcttg ttattattct tgactttgtg attgagttca ttttatacat
      421 gaaagatatg atgatggata aacaggagat aaaaagagaa tatatagagc aagagggaca
      481 ctttgagaca aagtcgagaa ggcgtgagtt gcatatcgag attctttcag agcagactaa
      541 atctgatata cgtaattcaa aattagtggt aatgaacccg actcatattg caattggtat
      601 ttattttaat ccagaaatag cgcctgcacc ttttatttct ctcattgaaa ctaaccagtg
      661 tgccttggct gtcagaaaat atgcaaatga agttggtata ccgactgtgc gtgatgtgaa
      721 attagctaga aaactatata aaacacatac aaaatatagt tttgttgatt ttgaacactt
      781 ggatgaagtc ctacgtctta ttgtttggct tgagcaggtt gaaaacactc attaataagt
      841 aaaggagatg tgttatatgg gagttaattt ctgtaataaa ataggtattg atcagtctga
      901 atttgaaata gaatcttcca tcattaactc cattgctaat gaggtattga acccaatatc
      961 tttccttagc aataaggata taataaatgt tttactcaga aagatttcat ctgaatgtga
     1021 cctggttaga aaagacattt atcgttgtgc tctggagttg gtcgttgaaa aaacacctga
     1081 tgatttataa tcattcataa taactctttt gctgatataa atat
//
LOCUS       DAZPAB010000434         1119 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZPAB010000434 DAZPAB010000000
VERSION     DAZPAB010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.443"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            64..243
                     /locus_tag="ACHUPG_004397"
                     /pseudo
     CDS             64..243
                     /locus_tag="ACHUPG_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(260..950)
                     /locus_tag="ACHUPG_004398"
                     /pseudo
     CDS             complement(260..950)
                     /locus_tag="ACHUPG_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1029..1119
                     /locus_tag="ACHUPG_004399"
                     /pseudo
     CDS             1029..1119
                     /locus_tag="ACHUPG_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          301 a          252 c          285 g          281 t
ORIGIN      
        1 gctcatttat tcctattata gaatgacaaa ttaaaattac tgtaaaaaaa cgatacacat
       61 ttaatacgag ggaggccggc agaaaaacgg cttgcggtca ggcaggagaa atgtgtgccg
      121 cgaatgaagt ccttgtatga ctggataaag ctccagagga aaactttgtc aaaccatgct
      181 gaaatggtaa aggcgttcaa ctatatcctg aatcactgga atgcactgaa tgagttttgc
      241 cgggtagtac ttccaatcat tagaatatgt gcttttcaat gaaggaaccg atgacttttt
      301 cgtggatttc cacggaacgc gagaagcaga ttgttttacg tgccagccgc ttgatgcggg
      361 ggcggagggt caggttgtta cgttcatacg ttgcgtgaag attttgccgg tcaggtgttt
      421 ctcctccggc acttctctgg cgtagctgcc ccagtcatcg ctggtcacca tgccgataca
      481 gaacggagtc aacagggcta acagttcccg acaggtctca tcattccgtg gcccgcaagt
      541 gtaagccaac acaccgccag ttttagtgtt gtacgcatac cagggccagt gttgttgcgc
      601 cttgctgcca acgaagcgcc actgctcatc aagttcacag ataagggcaa catcagcgtg
      661 agcgactggc gcagatgtta tccgccgggg cgagagcttt ttaatgtccg tatgacggta
      721 ttgatgccga ttttcagtgt cctggcggta tcacggcccc ctgcgccgtt atgtgccatt
      781 tcaacgatct gctccttaac gccgagtttt ctggcttcat aagagtaagt gagctggaaa
      841 acacgtttac atgagcgaca acggaaacgc tcatgccggg aacaactacg gccatgtcga
      901 taaacttcat cggaattaca gcgcggacaa tgaacagtaa cggtagtcat ggaaaacctc
      961 aaataccagt agattaccag aaacttctat cgattgaaga cattaccttt tgccgcaata
     1021 ggtggggtaa cattggtgaa aacgcattac gccgggtggc agttggaaaa aaattatctt
     1081 ttttcggctc tgatcgtggt ggagaaaatg cggcaataa
//
LOCUS       DAZPAB010000435         1109 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZPAB010000435 DAZPAB010000000
VERSION     DAZPAB010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.444"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1..1019
                     /locus_tag="ACHUPG_004400"
                     /pseudo
     CDS             1..1019
                     /locus_tag="ACHUPG_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            992..>1109
                     /locus_tag="ACHUPG_004401"
     CDS             992..>1109
                     /locus_tag="ACHUPG_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863705.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQV"
BASE COUNT          312 a          271 c          258 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggtta
//
LOCUS       DAZPAB010000436         1092 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZPAB010000436 DAZPAB010000000
VERSION     DAZPAB010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.445"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            2..>1092
                     /locus_tag="ACHUPG_004402"
     CDS             2..>1092
                     /locus_tag="ACHUPG_004402"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR0863706.1"
                     /translation="MLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQIIND
                     ELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVNMVAD
                     LWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQI
                     CWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQP
                     LHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLA
                     PLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREG
                     YTKVQNASYQVAAYLADEIAKQGPYEFICT"
BASE COUNT          255 a          292 c          292 g          253 t
ORIGIN      
        1 gctgttaacg gatttccgct cagaactact cgattcacgt tttggcgcaa aggccatttc
       61 tactatcgcg gagtcaaaac gatttccgct gcacgaaatg cgcgatgatg tcgcattcca
      121 gattatcaat gatgaattat atcttgatgg caacgctcgt cagaacctag ccactttctg
      181 ccagacctgg gacgacgaaa acgtccataa attgatggat ttgtcgatca ataaaaactg
      241 gatcgacaaa gaagaatatc cgcaatccgc agccatcgac ctgcgttgcg taaatatggt
      301 tgccgatctg tggcatgcgc ctgcgccgaa aaatggtcag gccgttggca ccaacaccat
      361 tggttcttcc gaggcctgta tgctcggcgg gatggcgatg aaatggcgtt ggcgcaagcg
      421 tatggaagct gcaggcaaac caacggataa accaaaccta gtgtgcggtc cggtacaaat
      481 ctgctggcat aaattcgccc gctactggga tgtggagctg cgtgagatcc ctatgcgccc
      541 cggtcagttg tttatggacc cgaaacgcat gattgaagcc tgtgacgaaa acaccatcgg
      601 cgtggtgccg actttcggcg tgacctacac cggtaactat gagttcccac aaccgctgca
      661 cgatgcgctg gataaattcc aggccgacac cggtatcgac atcgacatgc acatcgacgc
      721 tgccagcggt ggcttcctgg caccgttcgt cgccccggat atcgtctggg acttccgcct
      781 gccgcgtgtg aaatcgatca gtgcttcagg ccataaattc ggtctggctc cgctgggctg
      841 cggctgggtt atctggcgtg acgaagaagc gctgccgcag gaactggtgt tcaacgttga
      901 ctacctgggt ggtcaaattg gtacttttgc catcaacttc tcccgcccgg cgggtcaggt
      961 aattgcacag tactatgaat tcctgcgcct cggtcgtgaa ggctatacca aagtacagaa
     1021 cgcctcttac caggttgccg cttatctggc ggatgaaatc gccaaacagg ggccgtatga
     1081 gttcatctgt ac
//
LOCUS       DAZPAB010000437         1081 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZPAB010000437 DAZPAB010000000
VERSION     DAZPAB010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.446"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..847)
                     /gene="rhtA"
                     /locus_tag="ACHUPG_004403"
     CDS             complement(<1..847)
                     /gene="rhtA"
                     /locus_tag="ACHUPG_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR0863707.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLT"
BASE COUNT          321 a          285 c          268 g          207 t
ORIGIN      
        1 ctgtcagcgt agaccccatt gaagcggcga tgatagcgcc gagcgccagc agctgtatgg
       61 gtgtcagtgt ttctccgagg aaaatcatcc cggaaacggc agccagcgcc ggttccatgc
      121 tcatcagcgt accaaatgtc cgtgttggca aacgggtgag ggcaatcatt tccagcgaat
      181 aaggcagagc ggtcgagaga ataacgacag ccagacccaa tggaataacc gaccagtgcc
      241 agagtgcttc accagcctga agcgctccaa ttggcacgaa aattaacgct gcaatcaacg
      301 aaccaattgc taccgtcgca gggccatgtt ccgctcctgc gcgttgccca cttaaaatgt
      361 aaatagccca acaagccccg gcccccagtg ccagcgcaca gccggttaaa tcgacatggg
      421 aaacgtcttg ccccagcggt agcaggaacc acagaccaag aaccgccagc acaacccaga
      481 cgaaatctac cgggcgacga gaagagaaca gcgccaccgc cagtggtccg gtgaactcca
      541 gcgccaccgc aatacccagc ggtactgtct gaatagaaag ataaaaaaga taattcatcc
      601 cacccagcga aacgccgtaa aacaacagcg gtaaccgttg ctctttggca aagcgcagtc
      661 gccatggctt aaagaacgcg atgaggatca gcgttcctaa tgccagacgc agcgcagtga
      721 cacccggtgc gctcaccaga ggaaaaagtg acttagctaa cgaggctcca ccctgaatag
      781 acgccatggc aacgagcaat atgactattg gtaaccagac cggcatttta cgtaatgaac
      841 caggcatcct ttctcccaca aatatctaga cttaagtaaa gcgtggagtg tactggatat
      901 acccaatgct ggttgagcat ttgttgaaaa aattttcccc cgttttgact aaaatgcgcc
      961 aggattgatg gaatcattag tctggtgatt aggaataatc tggatgaatg acagggaaaa
     1021 catgcgtaat acttacgcag ttctctgaaa aagtgattta aatttagatg gatagcggtg
     1081 t
//
LOCUS       DAZPAB010000438         1075 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZPAB010000438 DAZPAB010000000
VERSION     DAZPAB010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.447"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..1038
                     /locus_tag="ACHUPG_004404"
     CDS             <1..1038
                     /locus_tag="ACHUPG_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR0863708.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
BASE COUNT          295 a          262 c          285 g          233 t
ORIGIN      
        1 ttctttccga accttgttga tcaccttctg gttaagcatc acacggctga tggcgttgaa
       61 gatggcattt caatctgcta cttcaagcca tactcgcaag gccgtgctcg ctggcagggt
      121 gacacaatcc acggcgtgtg gtttgacgaa gagccaccat acagcattta tggcgaaggt
      181 cttacccgta ccaacaaata cgggcaattc tcaattctga cgtttacccc gctgatgggg
      241 atgtctgacg ttgttaccaa gttcctgaag aaccccagca agtcgcagaa agtggtcaac
      301 atgaccatct atgacgctga gcactatacc gacgagcaga aagagcaaat catcgcatca
      361 tatcccgagc atgagagaga ggcgcgtgct cgcggtattc ctacgatggg tagtggtcga
      421 atcttccaga taccggaaga gacgattaag tgtcagccgt tcgagtgtcc tgatcacttc
      481 tacgtaattg gcgggatgga tttcggatgg gatcacccac aggcgcaagt tcagctttgg
      541 tgggataagg aggcagacac aatctacgtt tcacgcgtgt ggaaggcgaa agaaaaaaca
      601 gccgttcagg cgtggggagc cgttaaatca tgggcgcata aagtgccaac cgcatggcct
      661 catgatggaa accagcacga gaagggcggc ggtgagcagc tcaaagggca gtatgcagac
      721 gctggattta tgatgttgca ggagcatgcg acatggcctg atggcggtaa cgctgttgag
      781 ccaggcatca ctgaattgcg cgacatgatg ctcgatggtc gcttcaaagt attcaatacc
      841 tgtgagccat tctttgagga gttccgcctc tatcaccgtg atgaaaacgg gaaaatcgtc
      901 aagcttaacg acgacgtgct atccgccgtt cgctatgcat acatgatgcg ccgcttcgca
      961 aaaatgatgc gcgacatcaa aaaaccaaaa gagaaaaaga taccagcccc aatcaggccc
     1021 atcgcacgga gaacttaaat ggccgacgaa aacagactca attccattct gtgta
//
LOCUS       DAZPAB010000439         1073 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZPAB010000439 DAZPAB010000000
VERSION     DAZPAB010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.448"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..349)
                     /locus_tag="ACHUPG_004405"
     CDS             complement(<1..349)
                     /locus_tag="ACHUPG_004405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863709.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYP"
     gene            complement(495..>1073)
                     /locus_tag="ACHUPG_004406"
     CDS             complement(495..>1073)
                     /locus_tag="ACHUPG_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863710.1"
                     /translation="IYFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFS
                     TLFVILFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQ
                     NADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTIN
                     PLKDHLGYAYIDLTLSLFCCSQYSYIYSLRKQ"
BASE COUNT          423 a          201 c          165 g          284 t
ORIGIN      
        1 atgggtaaat gggtgataaa tacctgtcac cttgcgtaac agaaaaccaa aataagacat
       61 atgttaaaac aatcgagaaa aatacccaaa gtattttatt attcctaagt gtaaagacag
      121 acagtatagc ataaaaaaca acatcactgc caactttatt aattgcttgt tgcaaatcta
      181 atgtggcagg acttatacca tgtcgagctt gttctgctgt ttgaacttgt agatcaattt
      241 cattccatag gtagtgacca aatatatttc ctccagcagg actaaaagga tcaccagaga
      301 tccaaaaact acggacatac caataaatgc caccaagaat tgttgccaag ctaaaaataa
      361 gaaatgtctg tatgtttctc tttttcgcta tcaatataac aaatatgatg gctccatata
      421 tggcaccaaa tagttttacg ccacaagcaa ttccaataaa aaaaccacat aaaaaaatta
      481 attcattttg tgttttattg ttttctgagc gaatatatat aagaatactg gctgcagcaa
      541 aacaaagata gcgttaaatc aatatatgca taaccaagat gatctttaag tggattgata
      601 gtaaatagta ggattgtagc aataatgcct ggataataag ttttcgtaat ttcccatgaa
      661 cagccaacaa gtccacaggc tattacaaaa aaacagatat ttgctaaaaa ctgagctaac
      721 cttacatctc ctaactgtaa acctaaagcc attaaaagat ctgcattttg cgggaacaga
      781 ggaaaacgca aatattgttc aacaacaatt gcatggtgct caactaatga acgagccaaa
      841 ggtaagtgat acatagtatc atcccaatca ccgggagctc gcagggacga taacaatgta
      901 accacaaaaa tcaaaacaag aaataaaata acaaacagcg tactgaactc aatattattt
      961 attgccagcc atctaaattg ctgacgttta ataatgctta aaacccaaca caccgtaaaa
     1021 attaaaaaga tagcaatctt attaaattgc ccgaccatgc ccagaaagta aat
//
LOCUS       DAZPAB010000440         1070 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZPAB010000440 DAZPAB010000000
VERSION     DAZPAB010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.449"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..562
                     /locus_tag="ACHUPG_004407"
     CDS             <1..562
                     /locus_tag="ACHUPG_004407"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HFR0863711.1"
                     /translation="NTKGDAMIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTD
                     NYQAIQNAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAK
                     SGLTGSVLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVM
                     VNEVETALFNARALALKIYSMSGWNL"
     gene            789..>1070
                     /locus_tag="ACHUPG_004408"
     CDS             789..>1070
                     /locus_tag="ACHUPG_004408"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HFR0863712.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          315 a          202 c          257 g          296 t
ORIGIN      
        1 taacaccaaa ggcgatgcca tgattggcgt caagcagcct ttaccaaaag cagttttaag
       61 aactcagcat gacaaaaata aagaagcaat aagtatcctg gattttggtg ttattgatga
      121 tggtgtgaca gataattacc aggcaataca aaatgctata gatgccgttg cttcactacc
      181 ctccggcggg gagctgttta tccctgcgag caaccaagcg gtggggtata ttgttggatc
      241 cactttgctt attcctggcg gtgttaacat cagaggagtt ggtaaggcat cgcaactccg
      301 agcaaaaagc ggacttacag gatctgtgtt aaggctgtct tatgattcag acactatcgg
      361 ccgttatctg agaaatatac gagtaactgg taataacacc tgcaatggta ttgacacaaa
      421 cattacagca gaagactctg tcatcagaca ggtttatggc tgggtatttg ataatgtaat
      481 ggtgaatgaa gttgaaaccg cattatttaa tgcaagggct ctggcactca aaatttatag
      541 catgtcaggc tggaacctgt agagtcggtc ttcacttttt aggccagtgc gtaagtgtta
      601 gtgtcagctc ctgccatttc agcagaggaa attattctgc tgatgaaagc tttggcatca
      661 ggattcagcc tcaaacgtat gcgtggtcgt cagaggcagt taggtcagaa gcaataattt
      721 tagacagtga gaccatgtgc attggtttta aaaatgccgt ctatgttcat gattgccttg
      781 atttgcatat ggaacaactg gatttagatt attgcggctc aacaggcgtg gttatagaga
      841 atgtaaacgg aggattttct ttctcaaact catggatagc agcagatgcc gatggcactg
      901 aacaatttac ggggatatat tttagaacgc cggcctcaac gcagtcacat aaaattgtca
      961 gtggtgttca tatcaacact gcaaacaaaa acacggctgc aaacaatcaa agtatagcga
     1021 tagaacagtc ggcgatcttc gtctttgtaa gtggttgtac gttaactggt
//
LOCUS       DAZPAB010000441         1069 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZPAB010000441 DAZPAB010000000
VERSION     DAZPAB010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.450"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(227..652)
                     /locus_tag="ACHUPG_004409"
     CDS             complement(227..652)
                     /locus_tag="ACHUPG_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFR0863713.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            complement(649..1032)
                     /locus_tag="ACHUPG_004410"
     CDS             complement(649..1032)
                     /locus_tag="ACHUPG_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFR0863714.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
BASE COUNT          253 a          308 c          265 g          243 t
ORIGIN      
        1 ctcgcgtatg gttgattgac accacagtgc gaattcggcg aatgcgagat cacggtacga
       61 ttccgcacca cactaccaat caataccccg ggttttattc aggtaagcag gactgcggat
      121 atccggtgtc gcgcctttgt gtcacgaacg gggtgggtgc gaaacaccag ataaaataca
      181 ggttggcaat acctctgaac gccctgagca ggtcgggcat ttcggattaa gtactcgcac
      241 ctccgcagtc ctcaaacaag tctggctggt agctgtaaac agacttcgta cataccgctc
      301 tgcaataaat ccccgtacca caggcctcac agaacttctt cccatgaaaa aactgctcac
      361 acatcacaca atgccactcc agcacgaccg gtaatggcga taaaaacatc gccatatcct
      421 caatgtaagg gtgggacttc tccggattca gcaccacaca gtccgccttc tgttgctcac
      481 tcagggcatg taaatcatgc tcaaaccacg ccccctgagc atctgtctgc agaatcaacc
      541 ggccatggca ggaaaggcag aaacactgcc ggatatttct gctgagcgca gagtcggcac
      601 tgataatgtg ctcacccggc gtgatcccca gccccgtttt tataccgttc attctgctcc
      661 cccctcctca ctgaagcgcc cggtctggca gtgtgtgcag tactgctccc cataataatc
      721 gtggtgacat tgcctgcagt gccagctggc tttacgtact acgggtaagg tatccgggac
      781 gaattgctgc agacgtttaa tcaaccgtat ttctctgcgt tccggcctga cataagggca
      841 ctcatgaccg tgctccgtca gcccttcgtc agcgtgttca aaccagggcc gttcagtgtc
      901 gtattgcggg tggtatctga gtgcactgcc gcaaaggtgg caggtgtagc tatcgtaagg
      961 tgcagtctgt gcggtacggg caccggtcag acgtccgttg ccatcaagtg cgagaaaaga
     1021 ttttgcgtac atagtatatg ttcctcagcc gccagacgac acgcaggca
//
LOCUS       DAZPAB010000442         1066 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZPAB010000442 DAZPAB010000000
VERSION     DAZPAB010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.451"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(570..749)
                     /locus_tag="ACHUPG_004411"
                     /pseudo
     CDS             complement(570..749)
                     /locus_tag="ACHUPG_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            843..>1066
                     /locus_tag="ACHUPG_004412"
     CDS             843..>1066
                     /locus_tag="ACHUPG_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0863715.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAA"
BASE COUNT          285 a          193 c          277 g          311 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc cccctgaatc tccagacaac
      781 caatatcact tatttaagtg atagtcttaa tactagtttt tagactagtc attggagagc
      841 agatgattga tgtcttagga ccggagaaac gcagacggcg tactacacag gaaaagatcg
      901 ctattgttca gcagagtttt gaaccgggaa tgacggtctc ccttgttgcc cggcaacacg
      961 gtgtggcagc cagccagtta tttctctggc gcaagcaata ccaggaggga agtcttactg
     1021 ctgtggccgc aggagaacag gtcgttcctg cctctgaact tgctgc
//
LOCUS       DAZPAB010000443         1051 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZPAB010000443 DAZPAB010000000
VERSION     DAZPAB010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.452"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(108..479)
                     /locus_tag="ACHUPG_004413"
     CDS             complement(108..479)
                     /locus_tag="ACHUPG_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863716.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(540..>1051)
                     /locus_tag="ACHUPG_004414"
     CDS             complement(540..>1051)
                     /locus_tag="ACHUPG_004414"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863717.1"
                     /translation="LGKTMRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSP
                     SNYDISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTF
                     AGYYVIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDN
                     GLKIMYLSN"
BASE COUNT          249 a          271 c          250 g          281 t
ORIGIN      
        1 cataccttgg cctaggtaaa gaagatcaga ctcgtagcca catcggtggc aactgagctt
       61 gtacgcaatg cccatctggt tgtctcacct gaatcgtaac gtcagagcta ttcatccatc
      121 cattcggcat caatgtatgc cactaagccg accagctgac cgtttggact gaagtatgtt
      181 gcctgcaccc cacttgacat ctcatcaata ccaccgcact gcgctaggcg ccggtcccac
      241 ttgcccacag aagcgtcagg atcggctgag ttccccaaga cccttgtgag ccatgcctcc
      301 tttaactcaa cctctcgaat gattgcgtcg gccagctcct ccgcgttatc tagttcgaca
      361 tagtctggaa ttcggacgta cgttctgaac acagctggat acttacttgg gcgattgtca
      421 tacatgacat caacaatgta cccgtttgtg taaccgtctc ttggaggttc gtatgacact
      481 agtggttccc ctcagcttgc gactagatgt tgaggcctaa cattttatta gagagcaggc
      541 tagttgctta gatacatgat cttcaggccg ttatctgtca gggcaagcga aaattggcca
      601 tttatgacga ccaatgcccc gcagaagctc ccatctttgc cgccatagac gccgcgcccc
      661 ccttttgggg tgtagaacat ccttttgcca gatgtggaaa agaagttcgt tgtcccattg
      721 ttggcaatga cgtagtagcc ggcgaaagtg cgagacccat ttgcgctata tataagccta
      781 cgatttccgt tgcgactatt gtcgtaattg gatgaactat tatcgtagtt gctctcagag
      841 ttgtcgtaat ttgatggact attgtcgtaa ttgcttatgg agttgtcgta gttgcttgga
      901 gaaatgtcgt agttggatgg ggagtagtca tagggaagac gagcttcatc cactaaaaca
      961 attggcaggt cagcaagtgc ctgccccgat gccatcgcaa gtacgaggct tagaaccacc
     1021 ttcaacagat cgcgcatagt cttccccagc t
//
LOCUS       DAZPAB010000444         1037 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZPAB010000444 DAZPAB010000000
VERSION     DAZPAB010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.453"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..1015
                     /gene="ydfJ"
                     /locus_tag="ACHUPG_004415"
     CDS             <1..1015
                     /gene="ydfJ"
                     /locus_tag="ACHUPG_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFR0863718.1"
                     /translation="ILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMG
                     TNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEK
                     VNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAI
                     PTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPS
                     TIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAG
                     IFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPS
                     RSL"
BASE COUNT          241 a          232 c          265 g          299 t
ORIGIN      
        1 catcttgctg gtgacgctgc gtattattca gggattgggt gcaggtgcgg aaatttccgg
       61 tgccggtacg atgctggcgg aatatgcgcc aaaaggtaag cgcggaatta tctcctcatt
      121 tgtggctatg ggaactaact gcggcacctt aagcgcaacg gcaatctggg cctttatgtt
      181 cttcattctc agtaaagagg aactgctggc gtggggatgg cgtataccgt tcctggcgag
      241 cgttgtcgtg atggtctttg ctatctggtt gcgtatgaat ctgaaagaaa gcccggtctt
      301 tgagaaggtt aacgacagca accaaccgac agcaaaacct gcacctgctg gtagcatgtt
      361 ccagagcaaa tccttctggc tggcaacagg gctgcgtttt ggtcaggcgg gtaactccgg
      421 tttaattcag actttccttg caggctattt agtacagacg ttattgttta acaaagcaat
      481 tccaacagat gcattgatga tcagttcgat tctcggcttt atgaccattc cgttccttgg
      541 ttggttatcc gataaaattg gtcgccggat cccgtatatt attatgaata cctctgcgat
      601 tgtgctggcg tggccaatgc tttctatcat cgtagataaa agctatgccc cgagcaccat
      661 tatggttgca ctgattgtga ttcataactg tgcggtgctg ggattatttg ctctggaaaa
      721 cattaccatg gcagaaatgt tcggctgtaa aaaccgcttc acccggatgg ctatttcaaa
      781 agaaattggt ggtcttatcg cttccggttt tggtcctatc ctggcgggta ttttctgcac
      841 catgacggaa tcctggtatc cgatcgccat tatgatcatg gcatattcag tgattggttt
      901 aatctctgcg ctgaaaatgc cagaggtgaa agaccgtgat ttaagtgcgc tggaagacgc
      961 tgcggaagat caaccgcgtg ttgtaagagc tgcgcaacct tccagaagtc tgtaaaccct
     1021 caatcccttc tcttacc
//
LOCUS       DAZPAB010000445          990 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZPAB010000445 DAZPAB010000000
VERSION     DAZPAB010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.454"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            82..>990
                     /locus_tag="ACHUPG_004416"
     CDS             82..>990
                     /locus_tag="ACHUPG_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HFR0863719.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGF"
BASE COUNT          301 a          206 c          228 g          255 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt
//
LOCUS       DAZPAB010000446          987 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZPAB010000446 DAZPAB010000000
VERSION     DAZPAB010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.455"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..735)
                     /locus_tag="ACHUPG_004417"
     CDS             complement(<1..735)
                     /locus_tag="ACHUPG_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009247 - glycolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HFR0863720.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVP"
     gene            complement(765..>987)
                     /locus_tag="ACHUPG_004418"
     CDS             complement(765..>987)
                     /locus_tag="ACHUPG_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFR0863721.1"
                     /translation="LAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLG
                     AAISWSIRVILDALLLQWLYTRMCSVYEN"
BASE COUNT          325 a          188 c          143 g          331 t
ORIGIN      
        1 aggcacatga ttttttctaa gcaaaagaaa tgcatttctt actcgataat aacgacgtac
       61 tggcgagtga attggtatgt taatcccaca gatttttact gacttatccc caatagcatg
      121 aaccatttct atatcaggaa tgatatgaac taatatgcca tagtttaagc atctaagaca
      181 ccattctgta tcgacataat caataaaaag ttcctcatcc atcattccaa caatctcaaa
      241 aacttctttt gaaaccatgg ttccggatga gattgttact gaacttttaa acggtgtctg
      301 tccttcagtg acatgaattt tctctcgaag accatttttt ttgatattac aaataggata
      361 ataatgtgac ttatctctat caataaaaac tgggccagct atttttgcat tttcattatc
      421 catatagcag gaaagttttt tgataaactc attgcgaata cggctatctt gatcaaaaaa
      481 aattatcttc tctgcccctt tttcaagaga atatcgaatc ccaatatttt gtgcttttgc
      541 tataccttca ttcctaccaa gacaaattaa atgaactttt tcaatgtctg ctaatttatc
      601 tttcaaggtg aaattagtcc tattatcgac tactacaacg taacaatttt gctcctttag
      661 ttcagtgatc agtgcattca gatttttaag ttcgggatta tatgtcacaa tgacagcgta
      721 aatattacta ttcattatcc aagtgactca gtaattggtt aattttagtt ttcatataca
      781 gaacacattc tagtataaag ccattgtaat aacagagcat ctaaaattac acgtatgctc
      841 caggatattg cggcacccag taacccatat ttttcgacca taaaatacaa aagggctaaa
      901 taaggaacca attcagcaca atgaatcaaa gcagtaattt tagattttcc taatgcctgg
      961 attgcagaga atggtatcat tgccagc
//
LOCUS       DAZPAB010000447          982 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZPAB010000447 DAZPAB010000000
VERSION     DAZPAB010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.456"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            426..>982
                     /locus_tag="ACHUPG_004419"
     CDS             426..>982
                     /locus_tag="ACHUPG_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0863722.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
BASE COUNT          353 a          188 c          159 g          282 t
ORIGIN      
        1 gatgaaaaac aaagccaacg gtattctgat ttctgaagtt aatttcagac tcgtttaagc
       61 tccattttca aaaaaaatat atcagggttt gttactaata acttctgaaa acagaaaaaa
      121 atagagcagt aattctattt cagagaggag taactccatc tatcctcgat acgtactgaa
      181 cgttatcgtg atatattcat catgctttct tcaccattat tcgagtatag ggagagacgc
      241 aatgacctta aatacctttt tatggctgaa ttaccattct caggaataac agcagactcc
      301 ttcccataga cggcttaggg ggaaagcaat acccggagaa agggtacaaa gcaaactgtt
      361 ttaaaattaa taagccatat tctatttaac tagaattctt atcgttgata agaattctag
      421 ttataatgat taaatcaacc aatatacagg caatcggttc tggcattatg catcaaataa
      481 acaatgtata ctcgttaact ccattatctt tacctatgga actgactcca tcttgtaatg
      541 aattttattt aaaaacctgg agcgaatggg aaaagaacgg taccccaggc gagcaacgca
      601 atatcgcctt caataggctg aaaatatgtt tacaaaatca agaggcagaa ttaaatttat
      661 ctgagttaga tttaaaaaca ttaccagatt taccgcctca gataacaaca ctggaaataa
      721 gaaaaaacct attaacacat ctccctgatt taccaccaat gcttaaggta atacatgctc
      781 aatttaatca actggaaagc ttacctgcct tacccgagac gttagaagag cttaatgcgg
      841 gtgataacaa gataaaagaa ttaccatttc ttcctgaaaa tctaactcat ttacgggttc
      901 ataataaccg attgcatatt ctgccactat tgccaccgga actaaaatta ctggtagttt
      961 ctggaaacag attagacagc at
//
LOCUS       DAZPAB010000448          975 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZPAB010000448 DAZPAB010000000
VERSION     DAZPAB010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.457"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..104)
                     /locus_tag="ACHUPG_004420"
     CDS             complement(<1..104)
                     /locus_tag="ACHUPG_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HFR0863723.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(145..600)
                     /gene="csgB"
                     /locus_tag="ACHUPG_004421"
     CDS             complement(145..600)
                     /gene="csgB"
                     /locus_tag="ACHUPG_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFR0863724.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
BASE COUNT          263 a          234 c          174 g          304 t
ORIGIN      
        1 ccaccaccgt ggttaccgcc gccgccgtac tgaggaacaa cacctgccag agcgctaccg
       61 gagaatacga ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg
      121 attgatttaa aagtcgaatg gtaattaacg ttgtgtcacg cgaattgcca tttgcgactg
      181 tctctgcact acaactgccg ttttttgagt accatactgt gtaatatttg ctttattacc
      241 agaacctttc tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc
      301 gttggcactg cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt
      361 tgcccgattg ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg
      421 taactgagca ctattattag tcccagcctg accaattatg gctgcctgat taaatgaaga
      481 cttactcaat tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc
      541 ggctgcaatc ccaggcgcac ccagtattgt taacatcata aataacaatt tgtttttcat
      601 gttgtcaccc tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta
      661 acgcaacctg tattttgtta acgctgcgtt acgatggaaa gtatgtctgc ggaaatattt
      721 ttaataactc acccgcgcgt tgtatttttc tttttttgtt cataccaaag tattaaaacc
      781 cgcgtaaata cgctgttatc tacgcaaaaa atattttgtt ttctcttaaa tctccgtttt
      841 ccgctatcaa aaatcaccag acagtcattc ttcttgcccg tcgctgattg ctgcgatatg
      901 tcttgcgcac aagccgtgac aaacctcgtc tttttgcagt aaaaaaattg tccacggagg
      961 tgtggagaaa aaaca
//
LOCUS       DAZPAB010000449          965 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZPAB010000449 DAZPAB010000000
VERSION     DAZPAB010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.458"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..134)
                     /gene="yqcG"
                     /locus_tag="ACHUPG_004422"
     CDS             complement(<1..134)
                     /gene="yqcG"
                     /locus_tag="ACHUPG_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope stress response protein YqcG"
                     /protein_id="HFR0863725.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     "
     gene            273..944
                     /gene="queE"
                     /locus_tag="ACHUPG_004423"
     CDS             273..944
                     /gene="queE"
                     /locus_tag="ACHUPG_004423"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR0863726.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          272 a          197 c          230 g          266 t
ORIGIN      
        1 tggataaata aattgacggc ggcatcggta attttagatt cgttatcatt aaaaactaat
       61 acagaaaaaa taaatataat tttggtgaat gttttgacat gattaaatcc attttcttta
      121 ttttcttctg acataatacg ttactccctt taattattaa cgatatgttt cgtatttttc
      181 tttaaatctg catgtaacag aatgttaata agacatgatt gttgcggcag gatctgcaat
      241 aattaacaat tatccacttc acagagaatg ctatgcagta cccgattaac gagatgttcc
      301 agaccctgca aggtgagggt tactttaccg gcgttcccgc catttttatt cgtttacagg
      361 gatgcccggt tggctgtgcc tggtgcgaca ccaaacacac ctgggaaaag cttgaggatc
      421 gggaagtctc ccttttcagc attctggcga agaccaaaga gagtgataag tggggggcgg
      481 cgagcagtga agacttgctg gctgtcattg gtcgtcaggg atataccgcg cggcatgtgg
      541 tgattacggg tggtgagcct tgcattcatg atttgctgcc actgactgac ctgctcgaaa
      601 agaacggttt tagctgccag atcgaaacca gtggaactca tgaagtacgc tgcacaccga
      661 atacctgggt taccgtatcg ccaaagctga acatgcgcgg cggctatgaa gtgttgtcac
      721 aggcactgga gcgagccaac gaaatcaagc atccagtggg gcgcgtacgc gatattgaag
      781 cactggatga actactggca acgctgaccg atgataaacc gcgagtcatt gcactgcagc
      841 cgattagcca aaaggatgat gccacacgat tgtgcattga aacctgtatt gcgcgtaatt
      901 ggcgtttgtc gatgcaaaca cataaatatt taaatattgc ctgattacac atttataagc
      961 gttat
//
LOCUS       DAZPAB010000450          935 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZPAB010000450 DAZPAB010000000
VERSION     DAZPAB010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.459"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..132
                     /locus_tag="ACHUPG_004424"
     CDS             <1..132
                     /locus_tag="ACHUPG_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR0863727.1"
                     /translation="LKVDPLKCELRGGQMRFTGLKRGYRLAEQVMMHEPLARIRWCG"
     gene            319..556
                     /locus_tag="ACHUPG_004425"
                     /pseudo
     CDS             319..556
                     /locus_tag="ACHUPG_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            670..928
                     /locus_tag="ACHUPG_004426"
                     /pseudo
     CDS             670..928
                     /locus_tag="ACHUPG_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          292 a          176 c          254 g          213 t
ORIGIN      
        1 ctgaaggttg acccgctgaa gtgcgagctg cgcggaggtc agatgcggtt tacggggctg
       61 aagaggggtt accgtctggc agagcaggtt atgatgcatg agccgctggc tcgaatacgg
      121 tggtgcggct gagagtcgca gaggggaagt tgcgtccatg ttgaggggaa cggagcaaaa
      181 aaccatcaga gatacattga atcaatcaat gtaagcaata ttaagtgtct ataccgatta
      241 cttgatgatc aggttgtgtt gaataaacgc gcattcttcg gtgctttttt aatcttttgg
      301 ggatttaaat tcctatcgat gaactgtcga tatgaaaaag cgagcatcat actgacgtca
      361 aacaaagggg tcgcagactg gggagaaatg ttcggagatc acgtgctggc aacagcgatc
      421 ctagatcggc tgctacacgc tgaatatcaa aggagagagt taccggttaa aagagaaacg
      481 taaagctgga gtgctgacca aaaacacaac gccaatcagt gatgatgaaa tggtggaaaa
      541 cggacagcat cagtaacgaa agtatcttag caggcatgaa aatcgtccgc atcccctaaa
      601 cggtcaaaat gcttctgccg tgaaggaagg agaatgccat ctcatcactt atatctggcg
      661 aatgaaacac agatggtcgg ccttgccccc aaacacacgg cggtgcaaaa tggaatggca
      721 gaaaacttcg tcaaaacgat gaaatatgat taaatcagtg ttatgttcga acctgacgtg
      781 ttaacagcag taaagtagtc tcgcgaaggt gttcaaacat tataatgaat ggcatcctca
      841 tagtgcgcag ggttatcgct tgctgcggaa atatctgaag cgacgtacca gtaatgggga
      901 aaatgatagc tggtttctga aaatatagag gaaaa
//
LOCUS       DAZPAB010000451          933 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZPAB010000451 DAZPAB010000000
VERSION     DAZPAB010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.460"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(55..243)
                     /locus_tag="ACHUPG_004427"
     CDS             complement(55..243)
                     /locus_tag="ACHUPG_004427"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863728.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            complement(540..778)
                     /locus_tag="ACHUPG_004428"
                     /pseudo
     CDS             complement(540..778)
                     /locus_tag="ACHUPG_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088287.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          245 a          181 c          217 g          290 t
ORIGIN      
        1 ccgcgctgat ggttcttatc cgttttattg aatcggagaa caaactgtga gctatcagcc
       61 tggtggtctg gttttgcctg atttaagccg ccgtgggtat ttgtccaaga atgcttcaag
      121 ctccaatcgt tttggcctgg tgaatggaga tgggttaagc aaagactctc gttgctggtg
      181 tactttttcg cgctggcgct caagcatgga taatgcgatt gcctgtagtt cttcgttagt
      241 cattgtctgc acctggaaag caatttaccg gagtacctca cactacggca aaaatgtttt
      301 attgttgcaa cttgcgtaat tattcaggtc agtttggaac gcactgtata aacataaata
      361 acctgatact gttgtgtgtt ttcaaagaaa acacaggtat ttttcacata aaccttcccc
      421 ttttcatatt cgaccactta atcaaaaaac ggttttatac ttgttgcccc cactggggtt
      481 aaatgtgaga catgtcgcac tttgtgccat gtgtcctcgc tgattttagg cgcggttcat
      541 tcattctttc tgttggcata aagtatggta tcggttgggc gaatgaaagg gactcgggtc
      601 atggtcagac aatacctgag gcgttaattc gttgaaagag attgtgaatg ggggaataac
      661 tgagtcttaa ctgctgtgcg atagcccgga cagatagttg ctcgtcgaat ctgagacgta
      721 atgattctgg caaataagtg cgaaagccgg tgatatctag tgttgtttga gagttcatcg
      781 ttcgcgtcca ttaatacaga tggacgcgat tgttgccaga caggataatt ttcacaagac
      841 atcgcagata gagtacttac gataattttt tattttatac aacagatgcc cacgaaaaga
      901 attattttca atagacacct gcattacaca aag
//
LOCUS       DAZPAB010000452          915 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZPAB010000452 DAZPAB010000000
VERSION     DAZPAB010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.461"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(189..884)
                     /gene="aqpZ"
                     /locus_tag="ACHUPG_004429"
     CDS             complement(189..884)
                     /gene="aqpZ"
                     /locus_tag="ACHUPG_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFR0863729.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT          273 a          272 c          207 g          163 t
ORIGIN      
        1 tcagcttatc cccttcggca accgggtcgg gcgcgaggta acgtcctgta agacgcgccc
       61 tcagcccaca tccatgtggg ctgccccggc cttcggggaa cgcttcagcg attttgacgc
      121 caccaatacc cgtgcggttt gtaatgtcga agataaaaca accagggcag ataaaatgcc
      181 aatgctaatt aatcacgctt ttccagcaga gtccggtaaa tcagaccacc gataatgccg
      241 ccgacaattg gcaccaccca gaagaaccac agttgttcta atgcccagcc gccctggaag
      301 atagcaaccg cggtgctgcg cgccgggtta acagaagtgt tagtcaccgg aatactgatt
      361 aagtgaatca gggttaaggc cagaccaata gcgatcggcg caaaacctgc cggagcgaat
      421 ttgtcggttg caccgtggat cactaacagg aagcccgcac tcaataccag ttcaactacc
      481 agcgcggaaa gcatggaata accgcctggt gaatgctcgc cataaccgtt agaagcaaaa
      541 ccgctggctg ccgcgtcaaa acccgtttta ccactggcaa ttaaatacag cagcgccgct
      601 gcaacaatac cgccgacaac ctgggcaatt acgtagccaa cgacttcttt tgccggaaaa
      661 cgtccgccag cccataaacc aatagtgacc gccgggttaa aatgaccacc agaaatatga
      721 ccaacagcga aggccatcgt cagaacggtc aaaccaaatg ccagcgccac gccggcaaaa
      781 ccaatgccta attccgggaa gcctgcggcc agtacagcac taccacagcc accaaaaaca
      841 agccagaaag taccaaaaca ttcagctgct aattttctga acatatccac ctcaattaaa
      901 aattgaccct gtgaa
//
LOCUS       DAZPAB010000453          914 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZPAB010000453 DAZPAB010000000
VERSION     DAZPAB010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.462"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..785
                     /gene="lpxM"
                     /locus_tag="ACHUPG_004430"
                     /gene_synonym="msbB"
     CDS             <1..785
                     /gene="lpxM"
                     /locus_tag="ACHUPG_004430"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR0863730.1"
                     /translation="RLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMM
                     AELAIRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMA
                     AMFHQQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEY
                     SEFADFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASAD
                     NKMIARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT          268 a          179 c          218 g          249 t
ORIGIN      
        1 gcagattgag caaaaaagct cgtcgcaggg cgacgattaa tttatcgctt tgtttcccag
       61 aaaagagtga tacagaacgg gaaataattg tcgacaaaat gtttgtcaca gcattgcaat
      121 ccatagtgat gatggcagaa ctggcaattc gtggtccgga aaagttccag aaacgagtgt
      181 tctggaaagg cctcgaaatt cttgaggaaa ttcggcacaa taacagaaat gtgatttttc
      241 tggttcccca tggctggagc gtggatattc ctgcaatgtt gctggcagct cagggggaaa
      301 aaatggccgc catgtttcat cagcaacgaa atccagtgat tgattatgtc tggaattctg
      361 tacggcgtaa attcgggggg cgcttacatt cccgggagga tgggataaaa ccatttattc
      421 agtcagtacg ccagggatac tgggggtatt accttccaga tcaggatcat ggtcctgaat
      481 acagtgaatt tgctgatttt tttgcgacct ataaagcgac attaccaatt attggacgtc
      541 tgatgaacat cagtcaggct atgattatac cgcttttccc ggtttatgat gaaaaaaaac
      601 atttcctgac tattgaagtt cggccaccaa tggatgcatg cattgccagc gcggacaata
      661 aaatgattgc ccgacaaatg aacaaaacag tggaaatttt ggtggggtca catccggaac
      721 agtatatctg ggttttaaaa ttgttaaaaa cgcgcaaatc aaacgaagcg gacccgtacc
      781 cttgaaatta taaagtacag gtatttccac tagttgtttc ttacaggtta ccaatcgaaa
      841 cacatccccc ttccgtgtgt tgcccgtttg gcacggagga ctgtgtagat gcagttcgtt
      901 aaatttaatt tatg
//
LOCUS       DAZPAB010000454          913 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZPAB010000454 DAZPAB010000000
VERSION     DAZPAB010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.463"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..257
                     /locus_tag="ACHUPG_004431"
     CDS             <1..257
                     /locus_tag="ACHUPG_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR0863731.1"
                     /translation="AIAPTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGL
                     GVDQHQKIDNYTMGLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            519..>913
                     /locus_tag="ACHUPG_004432"
     CDS             519..>913
                     /locus_tag="ACHUPG_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863732.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIV"
BASE COUNT          313 a          145 c          176 g          279 t
ORIGIN      
        1 atgctatagc acccacacct gtatatgaaa attattttaa taacactact gctttcagac
       61 caaaacctga agatatcttc aaatgtggac agacgctcaa aaaaactaaa cagaaatttg
      121 gccattatgg attaggggta gaccagcatc aaaaaattga taattatacc atggggctaa
      181 attcttggaa gatagcacct aacgaacgtg gtgataaaaa aggtgtccca gggcttagtt
      241 gttttcaacc tcaataaaac ttcatgagtg ctgtggtatc tctttttaat tttgccagta
      301 tttcataggt tttcttaatc gattgtagag gtatgtactg atgacgcctc tacaatattt
      361 agggactttc tctgctatga gataagaaat ttcatattat gctttaaggc tgaatgattt
      421 gtgcaaaata taattatttc cccatcgaat atgaggtgta tgtcataaga taaaatgtta
      481 actatgtatt cctcgcggtg aataatatga ggtaaaagat gaaaattgga actgtggcag
      541 gcactaatgg cagtacaacg acaatcgcaa caaatgatat ggtgcaagag catgttacca
      601 acttcactaa agaactgttt gggtatattg caaatggtat aggtgatgat atatcaagta
      661 ttgccaggac aatgctcggt gaagttgtgg aaaaaattga tgattggcaa atagaacgtt
      721 tccaacaatc tattcaagat gacaaaattt catttaccat acaaacgaat cattcagaaa
      781 aatatagtat gctatcgggc atgcgcgctc atattctacg taggaataac tgttatcagt
      841 ttattgtgac aattaatagt aaaaattacg gttgccctct tgataatacc gatattaatt
      901 ggtgcagcat tgt
//
LOCUS       DAZPAB010000455          908 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZPAB010000455 DAZPAB010000000
VERSION     DAZPAB010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.464"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            100..293
                     /locus_tag="ACHUPG_004433"
                     /pseudo
     CDS             100..293
                     /locus_tag="ACHUPG_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
     gene            436..657
                     /locus_tag="ACHUPG_004434"
                     /pseudo
     CDS             436..657
                     /locus_tag="ACHUPG_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(678..740)
                     /locus_tag="ACHUPG_004435"
                     /pseudo
     CDS             complement(678..740)
                     /locus_tag="ACHUPG_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          280 a          220 c          207 g          201 t
ORIGIN      
        1 tttagctgaa cgggccatat aaccacctga aagacaatga cattgcctgt ttttataacg
       61 gtaattgcag accatgacaa accgcagccg tcaggctgcc tactcgagtc acaaggctct
      121 ttgtgtccga tatgccggca gcgcattacc catgaaactg gctggaacgt acaccataag
      181 gtgaagaaag ttatgggggc ggtgaagaat tatctaacct tgtcttgtta catccaaatt
      241 gccatcgaca gttacacagt tgtgaagccg gctctctcaa aaagggctta taaaggcttg
      301 agcggtgtac cgggaaaccg gtatgcaccg ttcttagggg agggcagtcc agcaatgaac
      361 tgtccttacc cgacaaacat ccaaaacgaa cggaacgtgc tggaatccgc atataacccg
      421 ttgtaaagtg aggaaatgga cacctcactt gctcatgaga acgcccgcct gcgggcactg
      481 ttgcagacgc aacaggacac tatccaccag atggctgaat acaaccgcct gctctcacag
      541 cggatggcgg cttatgcttc cgaaatcaac cgactgaagg cgctggttgc gaaactgcag
      601 cgtatgcagt tcggtaaaag ctcagaaaaa cttcgcgcaa aaaccgaacg gcagattcca
      661 ttttcacggg cgatctacgc cacacaggct ctgggttgca gtgactgctc cattaaggcg
      721 attttgaact cataagggaa gtaacaaaaa aactgcaaaa gtacaataat tatatatggc
      781 caaactaacc tctattaaaa aacatcagtt tttcacactt agttgagctt catgaaaaaa
      841 tcatcgagga tttcattgca gaatacacat tccactaact ggaagcactc cataagagga
      901 atcagaga
//
LOCUS       DAZPAB010000456          897 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZPAB010000456 DAZPAB010000000
VERSION     DAZPAB010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.465"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>897
                     /locus_tag="ACHUPG_004436"
     CDS             <1..>897
                     /locus_tag="ACHUPG_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0863733.1"
                     /translation="SNNQQTSFDQPDWKTGVFKFDTLHLNNADFSISRNANVEGNISA
                     NKSAITIGDKNAYIDNLAGKNITNNGFDFKQTISTNLSIGETKFTGGITAHNSQIAIG
                     DQAVVTLNGATFLNNTPISIDKGAKVIAQNSMFTTKGIDISGELTMMGIPEQNSKTVT
                     PGLHYAADGFRLSGGNANFIARNMASVTGNIYADDAATITLGQPETETPTISSAYQAW
                     AETLLYGFDTAYRGAITAPKATVSMNNAIWHLNSQSSINRLETKDSMVRFTGDNGKFT
                     TLTVDNLTIDDSAFVLRANLAQA"
BASE COUNT          303 a          182 c          177 g          235 t
ORIGIN      
        1 aagtaataat cagcagacct catttgatca gcctgactgg aaaaccgggg tgtttaaatt
       61 tgatacatta cacctgaaca atgctgactt ttcaatatca cgcaatgcca atgttgaagg
      121 aaatatatca gcaaataaat cagctatcac aatcggcgat aaaaatgctt acattgataa
      181 tcttgcaggg aaaaatatta ctaataatgg ttttgacttc aaacaaacta tcagtactaa
      241 tctatccata ggagaaacta aatttacagg tggcatcact gcacataaca gccaaatagc
      301 cataggtgat caagctgtag ttacacttaa tggtgcaacc tttctgaata atactcctat
      361 aagtatagat aaaggagcaa aagttatagc acaaaattcc atgttcacaa caaaaggtat
      421 tgatatctcc ggtgaactga ctatgatggg aatccctgaa cagaatagta aaactgtaac
      481 gccgggtctc cactacgctg ctgatggatt caggctgagt ggtggaaatg caaatttcat
      541 tgccagaaat atggcatctg tcaccggaaa tatttatgct gatgatgcag caaccattac
      601 tctgggacag cctgaaactg aaacaccgac tatatcgtct gcttatcagg catgggcaga
      661 gactcttttg tatggctttg ataccgccta tcgaggcgca ataacagccc ccaaagctac
      721 agttagcatg aataatgcga tctggcatct aaatagccag tcatcaatta atcgtctaga
      781 aacaaaagac agtatggtgc gttttactgg tgacaatggg aagtttacaa cccttacagt
      841 ggacaacctt actatagatg acagtgcatt tgtgctgcgt gcaaatctgg cccaagc
//
LOCUS       DAZPAB010000457          870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZPAB010000457 DAZPAB010000000
VERSION     DAZPAB010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.466"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>870
                     /locus_tag="ACHUPG_004437"
     CDS             <1..>870
                     /locus_tag="ACHUPG_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR0863734.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKTEQELFDAM
                     KKGALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSE
                     NGLQDFTRSLTNLLNALGGSGKSLGESLGSLMTSMSHGVDSLTDISYRVRAFFDEVTL
                     AYRPLNDTQRKIV"
BASE COUNT          263 a          153 c          209 g          245 t
ORIGIN      
        1 tttaagttca gcagcagtta aaacagcagg tgtacttatc agctttcagg ctgtactatc
       61 cgcttatcag cgagtaatgg aagttggcct tcaacgtgca agtgctgaac gttcaattga
      121 tttcgtattt tctaaagatg caaatcaagt aaaagatttc acaaagtcat tagctcaaac
      181 aacgggttta gatatcgctg aactgcaatc acagtttgct ggtttcggtg cttcagctag
      241 agagtctcta ggcattcaag gttctgaaga actttttaga aatatgattg gctattctcg
      301 tctgatgggg cgaagtgaag aagagattaa gcgagcactg acagcattat cacagatggc
      361 tggtaagggt cagattatgg ctgaagagct taaggggcaa ctagctgaag ctgttcctgg
      421 tatggttcag gtattcgcta aagcaaccgg aaagactgaa caggaattat ttgatgctat
      481 gaagaagggg gctttaaaat ctgccgatac tcttcagaag gtcactcagg aactgaataa
      541 acaaatcacc gctaaaggtg gctggaaagc tatttcagaa agtacacagg cacaactagg
      601 gaacttgaaa aactcttgga ataccacgct tgatagtatc ttcagaggtt ctgaaaatgg
      661 tctgcaagat tttacaagaa gcttaaccaa tcttttaaac gctcttggtg gaagtggtaa
      721 gagtcttggt gaatctcttg gtagtctcat gacttctatg tcacatggtg tcgattcatt
      781 gaccgatatt agttacaggg taagggcgtt ctttgatgag gtgacattag cttaccgtcc
      841 actaaatgac actcagagga aaattgttga
//
LOCUS       DAZPAB010000458          867 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZPAB010000458 DAZPAB010000000
VERSION     DAZPAB010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.467"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..802)
                     /locus_tag="ACHUPG_004438"
     CDS             complement(<1..802)
                     /locus_tag="ACHUPG_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR0863735.1"
                     /translation="MNAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKL"
BASE COUNT          284 a          151 c          131 g          301 t
ORIGIN      
        1 gcaatttatt atttttatgt atgtatgcag atgaagccat gtcttcgtgt tttaataatt
       61 cacataataa actccaaccc ttttccctga tgcctatatg ccctgaaacg agcaatgaat
      121 aaagtacagc gcctactctt ccatctgtat cgccaaaata agccgtgttg gcattttgtg
      181 ggttaatcac accacattgt ttataaaagt ctattttaga aaacacagat ttaacatagc
      241 tatcaaactt atccttagga aacgtctctt tagagacatc ccaacgccag tcagctttaa
      301 aattaccgct aagaagagta aagaaatttt caggtatgat agaaagccca ttaactttat
      361 aaattatgtc tgatttatct ccggagaaat taataaaata ataaccatcc ttacactcaa
      421 tcttaactgt aatgggagac gataatttta tcacatggtt attgtctggt ataattgtaa
      481 agataaacgt gttagtagta ttatcggcaa aactattata ttcttgaata tctatagatg
      541 gctcactatt gtaaaaccat attttctcca tctgaagact atgttcattt accctccagt
      601 gaccatgttg caggaaatag atgatatgca tgccaaaatc gttatctatt tgcgaatcat
      661 tatccagctt atcaatttca actatctttg aacgatgtgt tttatcaacc ttgctatggt
      721 gttgatgatt attaacttta tctgaatgct tatctttatg gtttgaaggg atgtacttaa
      781 atatattgtc aattgcgttc ataaataacc cgcgatattt ttgacctgtt agtaaaaaat
      841 aaatgataca tgtctgaaag acatatt
//
LOCUS       DAZPAB010000459          846 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZPAB010000459 DAZPAB010000000
VERSION     DAZPAB010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.468"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(1..150)
                     /locus_tag="ACHUPG_004439"
                     /pseudo
     CDS             complement(1..150)
                     /locus_tag="ACHUPG_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(101..>846)
                     /locus_tag="ACHUPG_004440"
     CDS             complement(101..>846)
                     /locus_tag="ACHUPG_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0863736.1"
                     /translation="SRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDV
                     IRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYRA
                     KSLTFYGINGPIYHTRYCYWPISRLTGWVKINITTEDIIYRIVASSVRNRWGDPDIGG
                     LIIAAYQGEADGDKVIRLVRGQSYRGSRLGPVGISVPGTPTGTYIVSPQFFITGCSEH
                     SLPGSYCALSGVPDAHVSGAMPGLFIRTS"
BASE COUNT          200 a          230 c          210 g          206 t
ORIGIN      
        1 actgcctggt ccctgtcgtc aaaaaaaaac gtcacagtct actgcgccac tttcggtata
       61 acggggatta atgatttttt taatttccac ggtgcattcc tcacgatgtg cgaataaaaa
      121 gcccgggcat tgcgccagag acatgtgcat ccggcacccc ggacagggcg caatatgacc
      181 ccggtaatga atgctctgaa catcccgtaa tgaaaaattg tggggatact atatacgttc
      241 cggtgggagt accgggcact gaaatcccca ccggtcccag tcgtgagcct ctgtatgact
      301 gccccctgac aagtctgatg actttatcac cgtcagcttc tccctggtac gcagcaataa
      361 tcagcccgcc aatgtcaggg tctccccatc tgttgcggac agagctcgcc acgattctgt
      421 aaataatatc ttctgtggtt atatttattt tcacccagcc agtcagtctg gatatgggcc
      481 agtagcagta gcgggtgtga taaatggggc cgttaatgcc gtaaaaagta agggatttgg
      541 ctctgtaccg cggttctgtt gtctcagggc gtgcatcagt ccaccggatg ctgagcaccc
      601 cttcaaaccg tgtgtcgggt atgatgatgc cgtaggggcc agcaacggaa tattcacctg
      661 gcagcgcatt ccttacccag gccaggaaat cactcttagt gtcaaaacgg ataacatctt
      721 caggcagaaa agcacaccca aagccgaatg cgccgggtat cgccagacgg ccttttgtcc
      781 ggtcgtaaat gtcgctctgt gcttccatcg tggccgcact tttcagcccc agattatccc
      841 gggact
//
LOCUS       DAZPAB010000460          835 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZPAB010000460 DAZPAB010000000
VERSION     DAZPAB010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.469"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="ACHUPG_004441"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="ACHUPG_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HFR0863737.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
BASE COUNT          267 a          120 c          177 g          271 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt aagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cactt
//
LOCUS       DAZPAB010000461          834 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZPAB010000461 DAZPAB010000000
VERSION     DAZPAB010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.470"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..329
                     /locus_tag="ACHUPG_004442"
     CDS             <1..329
                     /locus_tag="ACHUPG_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR0863738.1"
                     /translation="VSETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNG
                     LLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVI
                     LKITYN"
     gene            complement(372..572)
                     /gene="glgS"
                     /locus_tag="ACHUPG_004443"
     CDS             complement(372..572)
                     /gene="glgS"
                     /locus_tag="ACHUPG_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFR0863739.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          252 a          186 c          155 g          241 t
ORIGIN      
        1 ccgttagtga gacgtttagt gtagtcatga caaaagatcc tggtgcggcc tgtactgagc
       61 aattcaatat tttagggagc tttttcacta cggatatttt gagtgattat agccatctgg
      121 atatgggtaa tggactgcta ttgaagatat ttcataaaga tggaacagca acggaattta
      181 accgcttctc acaatttgct tctttttcat cgtctagtgc accttcggtc accgcaccat
      241 tcagggcaga acttagtgcg aacccggcag agactgttgt tgagggaccg tttagtaaag
      301 acgtaatcct caaaatcacc tataactagc atctaataca aacactaaaa cgggccatca
      361 ggcccgttat atcagtgctc taactccagc tctcttgcct gcatcatctg ttggcaatac
      421 caggcataac gtgcgcaaaa ccaagttcta tgttcttcat ccatgttacc agtaacagcc
      481 agaatatcga ccgggcgacc ttctgcgtgc attctggcaa aactacgcgc caggaaatcg
      541 aaattattca aagaattaag actatgctcc ataagcacat cctccggtta tcgcatacct
      601 tttaaggcca tatgcttttt ctgatttcag tattccccaa tcagtgtagt gcctttaaac
      661 ataacgtgcg taaatatatt gtccccgatc acacttttta gtgaaaaata aaaaaccata
      721 ttaaaatact ttctcttcca gaacttctct aacatgtgtt attattaaca aagacaaatg
      781 taagcaggga aactattcca aggtaaatat tgcgtcacta aatggacatt ggag
//
LOCUS       DAZPAB010000462          827 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZPAB010000462 DAZPAB010000000
VERSION     DAZPAB010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.471"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>827
                     /gene="yfdF"
                     /locus_tag="ACHUPG_004444"
     CDS             <1..>827
                     /gene="yfdF"
                     /locus_tag="ACHUPG_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFR0863740.1"
                     /translation="SISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKKGISTCI
                     KHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPANYKD
                     KELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDES
                     GKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLT
                     QYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIR"
BASE COUNT          312 a          149 c          150 g          216 t
ORIGIN      
        1 tctatttcaa tcaacaatac cagcgcagct tacccagaat ccatcaatga aaataacaat
       61 gatgaaatta atggattagt acaagagttc aaaaaccttt ttaatggtaa gaaaggaata
      121 agcacctgta ttaaacatct acttgagctt ataaaaaacg ccatacgagt aaacgacgat
      181 ccttatagat ctaatattaa taattcctca gttacttata ttgatattgg ctccaatgat
      241 acagaccata ttactattgg tatcgacaac caagaaccaa tagaattacc tgcgaactat
      301 aaagacaaag aactcgtccg tactatcatt aatgacaaca tagttgagaa gactcatgat
      361 atcaataaca aggaaatgat cttcagcgca ttaaaagaaa tatatgatgg agatcctggt
      421 tttatcttcg ataagatatc acacaaactc agacatacgg taacggaatt tgatgagagc
      481 gggaaaagcg aaccaactga cttatttacc tggtacggta aagataaaaa aggcgactct
      541 ctcgctattg taattaaaaa taaaaacgga aatgattact tatctctcgg ttactacgat
      601 caggacgact accacattca aagaggaatt cgtattaatg gtgatagtct cacccaatat
      661 tgtagtgaaa acgccaggag tgcttcagcg tggtttgaaa gcagtaaagc tatcatggca
      721 gaatcatttg caactggttc cgatcatcag gttgtaaacg agctcaacgg ggaaagactg
      781 agagaaccta acgaagtttt taaacgttta ggtcgagcaa taagata
//
LOCUS       DAZPAB010000463          826 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZPAB010000463 DAZPAB010000000
VERSION     DAZPAB010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.472"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1..556
                     /locus_tag="ACHUPG_004445"
                     /pseudo
     CDS             1..556
                     /locus_tag="ACHUPG_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
BASE COUNT          223 a          200 c          190 g          213 t
ORIGIN      
        1 aatcaggtct gggtcgcaga ttcagttgtc caggccttcc gaaatcaacc gactgaaggc
       61 gctggtcgcg aaactgcagc gtatgcagtt cggtaaaagc tcagaaaaac ttcgcgcaaa
      121 aaccgaacgg tggatacagg aagcacagga gcgaatcagc gcacttcagg aggaaatggc
      181 ggaaacactg ggtgagcaat atgacccggt actgccatct tccctgcgcc agtcttcagc
      241 ccgtaaaccg ttaccagcct cacttccccg tgccccccgg gtcatccggc cggaagagga
      301 atgctgtccg gcctgtggtg gtgaactcag tccactggga tgtgatgtgt cagagcagct
      361 ggagcttatc agcagcgcct ttaaggttat cgaaaaacaa cgccctaaac tggcctgtcg
      421 ccggtgcgac catatcgtgc aggcaccggt gccttcaaaa cccattgcac gcagttatgc
      481 cggagcgggg cttctggccc atgttgttac cggaaagtat gcagaccatc tgccattata
      541 ccgccaaagt gatctactat tccacacagc tatttagctc tctttttgtt gagctcccat
      601 gattctttca tttattgtta ctaattattt tatgttatca tgatgttaga acacgctctg
      661 ctctgtgtct ctattttatc tatcatattc tgaggattaa ctacataaca aggcgcaaga
      721 tatatataca aaaacaaata ataccgttgc aataattcct tttagtaaag gaggtgtgat
      781 taatttcagg ggatttcaga gtaaaaattg tacaacatat taaaat
//
LOCUS       DAZPAB010000464          823 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZPAB010000464 DAZPAB010000000
VERSION     DAZPAB010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.473"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(1..422)
                     /gene="yhaB"
                     /locus_tag="ACHUPG_004446"
                     /pseudo
     CDS             complement(1..422)
                     /gene="yhaB"
                     /locus_tag="ACHUPG_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
     gene            complement(678..>823)
                     /gene="tdcR"
                     /locus_tag="ACHUPG_004447"
     CDS             complement(678..>823)
                     /gene="tdcR"
                     /locus_tag="ACHUPG_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFR0863741.1"
                     /translation="RYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSSDRTSFCECN
                     RFP"
BASE COUNT          253 a          160 c          151 g          259 t
ORIGIN      
        1 actttgcgca atacttagaa atcgtctcct taatacattc cagctctttt ttattgacgg
       61 ctgagcaagt acattgttca ccaaaaggtg caaaagtgcc tacatctaat gtttgtatga
      121 tataatcatc atgttctcct gtagaaatat ctatgatgaa tggactcata ttttccggtt
      181 ggattcggaa tcatgacaga aggaacatcg ccttgataga aattcaatgc agtaattgca
      241 gataatactc tccggagttc attacttata ctgtaaattt tattaacgtc attggtggag
      301 acaatttctg caaactttct ttttaccgtc caataatcaa tatttctatt gtgattgtta
      361 accactgcgt gcattggcgg gattgaaggg tgaaaaatat gtgcaattgg gaagcctttc
      421 attgatgcct gccttgtcgt catgacaaac aataatagat gcttattttc ctcgcacgac
      481 acttacgcac tatcaaaaaa cgctcattta cccggcgtta cagtaaacga agcaacattc
      541 tgtgtcctac gctgatgtaa agcacagtga aaatccccct tgttgacttc gtctgacctt
      601 atcgcggcga ccagcaatta ttcgttttgt gatcctcaac acaaaatgct attcgtcgcc
      661 tatgagggga gaagaattta tggaaaacga ttgcattcac aaaatgacgt cctgtcagaa
      721 gagaaaaagt tgacgttaat caattgttcg agatgatcaa tacctgtatt cgaaatttta
      781 aaatattgat aaaataaatg tttgttctcc aacaaatagc gtg
//
LOCUS       DAZPAB010000465          803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZPAB010000465 DAZPAB010000000
VERSION     DAZPAB010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.474"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            46..803
                     /locus_tag="ACHUPG_004448"
                     /pseudo
     CDS             46..803
                     /locus_tag="ACHUPG_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
BASE COUNT          234 a          169 c          198 g          202 t
ORIGIN      
        1 tactgcaccc attttgttgg acgatgaaat ggaatagccc ctgatatgtc aaagccaaaa
       61 tacccttttg aaaagcgcct tgaagtcgtg aatcactact tcactactga tgatggttac
      121 aggatcatct cggcacgttt tggtgtcccc cgaacccagg tcaggacatg ggttgccctc
      181 tatgaaaaac atggagaaaa aggcttaatt cccaaaccta aaggtgttag tgctgatcca
      241 gagttgcgta ttaaggtcgt gaaagctgtg attgagcagc acatgtccct taatcaggct
      301 gctgctcact ttatgcttgc tggtagtggt tctgtagcca ggtggctgaa ggtttatgaa
      361 gagcgcggag aagctggttt acgcgcgctc aagattggca ccaaaagaaa cattgcaata
      421 tcagttgatc cagaaaaagc ggcatcagca ttggagctgt caaaagaccg acgcattgag
      481 gatcttgaaa ggcaagttcg atttcttgaa acgcggcttg tgtatctaaa aaagctgaaa
      541 gccttagctc atcccacgaa aaagtgaaag tactcaacga gctaaggcag ttttatcctc
      601 ttgatgagct tctcagggct gcggagatac cgcgcagtac gttttattac catctaaagg
      661 ctctcagcaa gcctgacaag tatgcggacg ttaaaaagcg tattggtgag atttatcatg
      721 agaatagagg ccgatacgga taccgtaggg taacgctgtc tcttcatcga gaagggaaac
      781 agattaacca taaagctgtt cac
//
LOCUS       DAZPAB010000466          795 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZPAB010000466 DAZPAB010000000
VERSION     DAZPAB010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.475"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            20..736
                     /locus_tag="ACHUPG_004449"
     CDS             20..736
                     /locus_tag="ACHUPG_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0863742.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          260 a          135 c          169 g          231 t
ORIGIN      
        1 tttcgaggag atcattccag tgggacgtaa atgggccaat attgttgcta aaaaaacggc
       61 taaagacggt gcaacgtcta aaatttatgc aaaattcggt gtagaaatct atgctgctgc
      121 caaacaaggt gaacccgatc cagaattaaa cacatcttta aaattcgtta ttgaacgtgc
      181 aaagcaggca caagttccaa agcacgttat tgataaagca attgataaag ccaaaggcgg
      241 cggagatgaa acgttcgtac agggacgtta tgaaggcttt ggtcctaatg gctcaatgat
      301 tattgctgag acattgactt caaatgttaa ccgtacgatt gctaacgttc gcacaatttt
      361 caataaaaaa ggcggcaata tcggagcggc aggttctgtc agctatatgt ttgacaatac
      421 gggtgtgatt gtatttaaag gaacagaccc tgaccatatt tttgaaattt tgcttgaagc
      481 tgaagttgat gttcgtgatg taactgaaga agaaggtaat attgttattt atactgaacc
      541 tgctgacctt cataaaggaa tcgcggcgct aaaagcagct ggaatcactg agttctcaac
      601 aacagaatta gaaatgattg ctcaatctga agttgagctt tccccagaag atttagaaat
      661 ctttgaaggg cttgttgatg cccttgaaga tgacgacgat gtacaaaaag tttatcataa
      721 cgtcgcaaat ctctaattat attttaaaga aatctgtctt tatggcagat ttctttaatc
      781 tcatataatt cttat
//
LOCUS       DAZPAB010000467          791 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZPAB010000467 DAZPAB010000000
VERSION     DAZPAB010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.476"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            151..357
                     /locus_tag="ACHUPG_004450"
     CDS             151..357
                     /locus_tag="ACHUPG_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR0863743.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT          236 a          162 c          159 g          234 t
ORIGIN      
        1 cagtgtgaat gtaaataaac catcaataag caaaaataag tggtcggatg agtagtaaaa
       61 ttacacaaaa ggcggtacta ttttcatcag ataaacaaat tcatttttcc ggtgctattc
      121 accgggtaga tacaaaaggg ggagaaaagt atggaactct acagagaata tcctgcatgg
      181 cttatctttt tacgccgtac atatgcggtt gcagcgggcg ttctggcgct gcctttcatg
      241 cttttctgga aagaccgcgc ccgcttttac agctacctgc accgcgtctg gtcgaaaacc
      301 agcgataaac cggtgtggat ggatcaggcc gaaaaggcaa ccggtgattt ttactgattg
      361 cctgcgcgct taagccagcc tcgtatcagg gggctggttt aggtgttcaa catcaacgtc
      421 ttccatgata attaacatgg aagacgtttc catcgtatta ttcatttcat cactgtttta
      481 gcatcacgtt tcaatagtat cctttcatag ggctacccag gatgcggtca ccttcattgt
      541 cgctctaagc gactagatca tcagaaatct ccagttagat tgcatacttc cttacacact
      601 ttcgtgaaac ttcagggtaa ggtgagaaag ccgtcatccc tttttctaat tccagcatct
      661 aaataaattc cgaattttgt tattagtatc gctaccgcaa taaaaacaga tagtaaagag
      721 agtaatatat actttattga tatattaaga ataattaatt gttaacatat ctaatccaat
      781 aagatgttat g
//
LOCUS       DAZPAB010000468          782 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZPAB010000468 DAZPAB010000000
VERSION     DAZPAB010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.477"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..666)
                     /locus_tag="ACHUPG_004451"
     CDS             complement(<1..666)
                     /locus_tag="ACHUPG_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFR0863744.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALP"
     gene            complement(656..>782)
                     /locus_tag="ACHUPG_004452"
     CDS             complement(656..>782)
                     /locus_tag="ACHUPG_004452"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HFR0863745.1"
                     /translation="MRVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT          251 a          203 c          177 g          151 t
ORIGIN      
        1 tggcagtgct attttgattc gacgttgatc aagtttctga taattccaac gacggtcgac
       61 gggcaccatc cagtcaatca acatacctac cgccagccct gtgatgacat caataaaatg
      121 atgctgccat gtcgtcagcg tcgaaatggc gatgagtaaa aaccatccgc cacagacttt
      181 acgccacctc acagccagat gctgacgaaa gtgacgccaa agtagccagc agagaataat
      241 atgcagcgaa ggagactggt tataaggcag atcaaacagt tcaagttgcg aaaatagcca
      301 tcctgtcacc ccactcactt caggacggat aaaactaaac ttcagcgggt agagaaaaaa
      361 accgcagcag gccattaccg ttgccagaat aagccggtgg acaagtcggc gttgttcgaa
      421 tgtcgagcta caaacgaaca gcgaaaatcc atataaaaga tccagactcc agtaaggaac
      481 aatagtccag ggaagaaaag ggatcgccgt ttcccaaccg aatacctgac tggggatatc
      541 atggctgtta aggtcctgaa ccgcggtgaa ctgattaaga gatccatagg tgaagaagaa
      601 aaacggggcc agcaacaata accagccagc gccttgtagc aatacgttcc gacgttcagt
      661 tatcacgacg caccgccatc gaaaccgtaa aaatgcccca cacatcaatc cgttgagtgc
      721 atttatcaaa tccggcatta cgcacgagcg aatccatctc cccttgcgaa cgcacgcgca
      781 tt
//
LOCUS       DAZPAB010000469          772 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZPAB010000469 DAZPAB010000000
VERSION     DAZPAB010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.478"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..243
                     /locus_tag="ACHUPG_004453"
     CDS             <1..243
                     /locus_tag="ACHUPG_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0863746.1"
                     /translation="ELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGR
                     LKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            358..>772
                     /locus_tag="ACHUPG_004454"
     CDS             358..>772
                     /locus_tag="ACHUPG_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFR0863747.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPS"
BASE COUNT          206 a          198 c          201 g          167 t
ORIGIN      
        1 gagctgaaaa ataacggtat aaaggcagta atcccccgaa agtcaaatga gaaaatggca
       61 tcggatggac gtgcacagct tgatcgtgat gcgtactgca atcgtaatgt cgttgaacgg
      121 tgctttgggc ggctgaaaga atatcgccgc atcgccacgc gttacgacaa aacggcgaga
      181 aattacctgg cgatggtgaa actgggctgc atccgactct tttatcaacg cttacgtaat
      241 taagggacac agcctaaata gctgcgcgga atagtagatc cctttgaggg aacttagccc
      301 ggattgtgcg atctgatcaa tcgccaaatc aaaacaaatc accaaccgga ctgagcaatg
      361 ccgatcatag caccaatttc ccgtgacgaa cgacgcctga tgcagaaagc catccataaa
      421 acacacgata aaaattatgc ccgcagactg actgccatgc tgatgctgca ccggggcgac
      481 cgtgtcagcg acgttgccag aacgctctgc tgcgcccgtt cctctgttgg acgctggatt
      541 aactggttca cgcagtcggg tgttgaggga ctgaaatcat tacctgccgg gcgtgcccgt
      601 cgctggccgt ttgagcatat ctgcacactg ttacgtgagc tggtaaaaca ttctcccggc
      661 gactttggct accagcgttc acgctggagt acagaactgc tggcaataaa aatcaatgag
      721 ataaccggct gccagttaaa tgccggaacc gttcgccgct ggttgccgtc tg
//
LOCUS       DAZPAB010000470          762 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZPAB010000470 DAZPAB010000000
VERSION     DAZPAB010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.479"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..521)
                     /gene="fimE"
                     /locus_tag="ACHUPG_004455"
     CDS             complement(<1..521)
                     /gene="fimE"
                     /locus_tag="ACHUPG_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFR0863748.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTA"
BASE COUNT          244 a          181 c          146 g          191 t
ORIGIN      
        1 ctggcggtat aacgcacagt atggcgaata tttcgatgcc cgagataatc ctgaattaaa
       61 cgggtatctg caccacgctc cgccagttca taaccgcaag catgccttaa catatgagga
      121 tgagtctgcg ttacggttcc agcttcaata ccggcatcgc gaataatgcg ataggcctgc
      181 tggcgagaaa gccgactccc gcggcgagaa ataaatatag cgtcggtccg gtcagcgcct
      241 ttccagttag cacgttcctg ggtccagcgt tccacggctt cacgctcatc aaaacgtaac
      301 gggtgaacgg tagaaaatcc gttcttcagt cggcgaatat ttattcgacc ttcattaagg
      361 tcaaggtcct gataatgcag atcaagcagt tcactaatac gcatcccatg ccgatatgcc
      421 aacagaataa gacaataatc tctggctccc gttgccccgt aacaaaccgc ctgcatcatg
      481 gcctgaactt ctttaccggt aagataacga cgtttactca caatagtagt actcctaact
      541 gagatatatt tgaatgaata cctataggaa acctcaatcg gtcaaaatta gcccaataga
      601 taaaaagata accacacacg aagttatgtg gctatcagtc aattacatta aacatcatac
      661 aaaaataaaa tatcactgat gtgacaacat ctattttcat tgatttgatt caatataaca
      721 atagattaca gaggattaac ttccagttac ttaaaaaata aa
//
LOCUS       DAZPAB010000471          759 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZPAB010000471 DAZPAB010000000
VERSION     DAZPAB010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.480"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>759
                     /locus_tag="ACHUPG_004456"
     CDS             <1..>759
                     /locus_tag="ACHUPG_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR0863749.1"
                     /translation="ERILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFEL
                     DLEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGT
                     DLFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTID
                     GFSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGD
                     VVNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVA"
BASE COUNT          211 a          155 c          184 g          209 t
ORIGIN      
        1 gagcgcattc tgaccgaccg tgaaaatggt gaaaaaggcg ataattctca agatacctct
       61 tatgcccgtg taggcgtgaa aggtgaaact cagatcaacc ctgaaatgac tggttacggt
      121 cagtttgaac tagacctgga agcatcaaac agacacaacc ctgaccaaac tcgtttggca
      181 tatgctggtt tgagttacaa agattttggt tcattcgact acagtcgtaa tgtcggtgtc
      241 gcgtatgatg cagaagcatt tactgatatg tttgttgagt ggggcggtga ctcatgggct
      301 ggtactgacc tgttcatgac caaccgtacc aacggtgttg caacctatcg taacacagat
      361 ttctttggca tggttgaagg tctaaatttt gctctgcaat atcagggtaa aaatgaaggt
      421 acaggcaact acaaagcaaa tggcgatggc catggtttat ctgcaactta cactattgac
      481 ggtttctcat ttgctggtgc ttatgcaaac tctgaccgta ctgattggca gtctggtgat
      541 ggtaaaggcg aaagagccga agtttgggcg ttgtcaacaa aatatgacgc taacaatgtt
      601 tatgctgctg tgatgtacgg tgaatctcac aacatgaatt ccgacgacgg tgatgttgtg
      661 aacaaaacac aaaacttcga agctgttctg caatatcagt tcgactttgg tctgcgtcca
      721 tccatcggtt actcctattc taaagcactg gacgtagca
//
LOCUS       DAZPAB010000472          759 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZPAB010000472 DAZPAB010000000
VERSION     DAZPAB010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.481"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            403..478
                     /locus_tag="ACHUPG_004457"
     tRNA            403..478
                     /locus_tag="ACHUPG_004457"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:436..438,aa:Ile,seq:cat)
BASE COUNT          233 a          128 c          174 g          224 t
ORIGIN      
        1 tctggagagg caactgtcaa aaatcgcgca tacgaaacta atgattagca gaataagatc
       61 tatgaaagaa tatattgcta tatgaagttt atttttatgg agtgaaaagt aacagatatc
      121 ataatgatgc gaaaactctt cagctaatgt aataaagtta tattattaga tacaaaatca
      181 ttacaaagta tcattgaatc gatgataact atagatagtg ctaattatta acatttgctt
      241 gttgactgcg agtatcgact ttgcgaatgg aggcggtgtc gggttttatg tcgcgtgtaa
      301 tcacgaccca aggtgaatgg gaacgcataa aaatctgtta tttacatcat aagatcatca
      361 tgtttattgc gtggtttata agtattgtta ttgcatactt aaggcccttt agctcagtgg
      421 ttagagcagg cgactcataa tcgcttggtc gctggttcaa gtccagcaag ggccaccaaa
      481 tcatcattca tgtatcagga agagttaagg gtacaagggg tactcgatgg cggtctatat
      541 ttcatgacta acgccatttc aaggaagaag tgcggtgggt ggtttcgaat gagggctggt
      601 gttttggtac gtggcacgcc actgcaatgt atttattcat cagtactcat tcaaaagtac
      661 ctggaaaaaa tgcccgctat gccaagcggg caaattgatc ctacccacgt aatatggaca
      721 caggcctaag cgaggttctg gttttcaaat tgttccgga
//
LOCUS       DAZPAB010000473          757 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZPAB010000473 DAZPAB010000000
VERSION     DAZPAB010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.482"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1..704
                     /locus_tag="ACHUPG_004458"
                     /pseudo
     CDS             1..704
                     /locus_tag="ACHUPG_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
BASE COUNT          290 a          117 c          155 g          195 t
ORIGIN      
        1 ccactggtac tcaagaaaaa ataactaaag acaaagatat tgtgttcaca aatgggggaa
       61 gtgttctgtt taaggataat ttggattttg gttgcggtgg tattatcttt gacgaaggcc
      121 atgaatataa cataaacggt cagagattta catttaaagg agcaggaatt gatatcggaa
      181 aagaaagcat tgtaaactgg aatgcattgt attccagtga tgatgtttta cacaaaatag
      241 gccccggtac tctgaatgtt caaaaaaaac agggggcaaa tataaagata ggtgaaggaa
      301 atgttattct taatgaagaa ggaacattta acaatatata ccttgcaagc ggaaatggta
      361 aggtaatact aaataaagat aattcccttg gcaatgatca atatgcgggg atatttttta
      421 ctaaacgtgg tggcacgcta gatttaaatg gacacaatca gacttttact agaattgccg
      481 ccactgacga tggaacaaca ataactaact cagatacaaa gaaagaagcc gttctggcaa
      541 tcaataacga agactcctac atatatcatg ggaacataaa tggcaatata aaactaacgc
      601 acaatattaa ttctcaggat aagaaaacta atgcaaaatt aattctggat ggtagtgtca
      661 acacaaaaaa tgatgttgaa gtcagtaatg ccagtcttac catgtaaggc catgcaacag
      721 agcatgcaat attcagaagc acagcgagtc attgctc
//
LOCUS       DAZPAB010000474          745 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZPAB010000474 DAZPAB010000000
VERSION     DAZPAB010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.483"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..732)
                     /locus_tag="ACHUPG_004459"
     CDS             complement(<1..732)
                     /locus_tag="ACHUPG_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863750.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYS"
BASE COUNT          232 a          155 c          107 g          251 t
ORIGIN      
        1 agagtaatct tcatcctcca aaacaacaga ggaaccatta cgaaaacgtt catcccctcc
       61 atagatttta agcgcaaagc ccaaagattc tatgactatg gcaaaattat catcaattat
      121 tttttcgtaa atataccatc catcgcattc tttaattcca ccgcctggta tttggagttc
      181 aaatttaccc cgttttaatt tgcttactac atcctccaat aaggcataac cgaaagatac
      241 gtcaccaaac gcctggcgaa tataaccttt aatccttttt ggtatgtcag actgtttttt
      301 ctgcaaaata tcacaggcat acaatcttct gttttgttct aacggaaact cgcggttttt
      361 gaaatccatg aatgagttat actcatttat tgttatggga atttcttcgt attcaaaccc
      421 actgcctcgc aatgttattg tatattcagc accgttatct ttaaggctga aactgataag
      481 aaaattatcc atggcattaa cagtttcatt ttttgatgaa agcacttgat aactattttt
      541 atcaatctgt gttgcgcaac gaaaggactc ccgcaaagtt aacagactat cataattcga
      601 aacccaatct tttttcttaa atgcatgctg aatattatta aaaaaatcgt ttgaatgtgt
      661 ttttgtatgc gatgacttta caaataactc caccgcacca ataaaattat tgtgtctgat
      721 cctgggtaac atatggctac cttat
//
LOCUS       DAZPAB010000475          745 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZPAB010000475 DAZPAB010000000
VERSION     DAZPAB010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.484"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>745
                     /locus_tag="ACHUPG_004460"
     CDS             <1..>745
                     /locus_tag="ACHUPG_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR0863751.1"
                     /translation="GGQQEREMRRFAGACRFVFNRALALQNENHEAGNKYIPYGKMAS
                     WLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPRFKKRGQNAAFRYP
                     QDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWYISIQTESEVSTPV
                     HPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQ
                     KRKIQRLHSCIANIRRDYLHKVTTTVSKNH"
BASE COUNT          214 a          187 c          187 g          157 t
ORIGIN      
        1 ggtggtcaac aggagcgtga aatgaggcgc ttcgcaggcg catgtcgttt cgttttcaat
       61 cgtgctctgg cgcttcagaa tgagaatcat gaggccggta ataaatacat cccttacggg
      121 aaaatggctt cctggctggt tgagtggaaa aacgccactg aaacgcaatg gcttaaagat
      181 tcgccctcac agccattgca acagtcactg aaagaccttg agcgggctta caaaaacttc
      241 ttccagaagc gggctgcttt tccccgattc aaaaagcggg gacagaatgc tgcattccgc
      301 tacccgcagg acgttaagct cgatcaggaa aacagccgta tttttctgcc gaaacttggc
      361 tggatgcgct accggaatag ccgtcaggtc acgggagttg tgaaaaatgt cactgtcagc
      421 cagtcctgcg gtacgtggta catcagtatt cagacagaaa gtgaagtatc aactccggtt
      481 cacccttcag catcaatgat cgggctggat gctggcgtgg ctaaactcgc cacgctgtca
      541 gatggcacag tctttgagcc tgtaaacagt tttcaaaaaa accagaagac gctggcgaga
      601 cttcagcgac agttaagccg gaaggtcaaa ttcagcaaca actggcagaa gcagaaacgc
      661 aaaatacagc gactgcattc ctgtatcgca aatatccgca gggactacct tcacaaagtc
      721 acaacgaccg tcagcaaaaa ccacg
//
LOCUS       DAZPAB010000476          733 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZPAB010000476 DAZPAB010000000
VERSION     DAZPAB010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.485"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..158)
                     /locus_tag="ACHUPG_004461"
     CDS             complement(<1..158)
                     /locus_tag="ACHUPG_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFR0863752.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="ACHUPG_004462"
     CDS             complement(308..469)
                     /locus_tag="ACHUPG_004462"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863753.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(469..654)
                     /locus_tag="ACHUPG_004463"
                     /pseudo
     CDS             complement(469..654)
                     /locus_tag="ACHUPG_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
BASE COUNT          190 a          214 c          144 g          185 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taa
//
LOCUS       DAZPAB010000477          728 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZPAB010000477 DAZPAB010000000
VERSION     DAZPAB010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.486"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..615
                     /locus_tag="ACHUPG_004464"
     CDS             <1..615
                     /locus_tag="ACHUPG_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA
                     acyltransferase"
                     /protein_id="HFR0863754.1"
                     /translation="KRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKK
                     MAAMFHQQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGP
                     EYSEFADFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIAS
                     ADNKTIARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          225 a          145 c          169 g          189 t
ORIGIN      
        1 aaacgagtgt tctggaaagg cctcgaaatt cttgaggaaa ttcgacacaa taatagaaat
       61 gtgatttttc tggttcccca tggctggagc gtggatattc ctgcaatgtt gctggcagct
      121 cagggaaaaa aaatggccgc catgtttcat cagcaacgaa atccagtgat tgattatgtc
      181 tggaattcag tacggcgtaa attcgggggg cgcttacatg cccgggagga tgggataaaa
      241 ccatttattc agtcagtacg ccagggatac tggggatatt acctcccaga tcaggatcat
      301 ggtcctgaat acagtgaatt tgctgatttt tttgcgacct ataaagcgac attaccaatc
      361 attggacgtc tgatgaacat cagtcaggct atgattatac cacttttccc ggtttatgat
      421 gaaaaaaaac atctactgac tattgaaatt cggccaccaa tggatgcttg cattgccagc
      481 gcggacaata aaacgattgc ccgacaaatg aacaaaacag tggaaatttt ggtggggcca
      541 catccggaac agtatgtctg ggttttaaaa ttgttaaaaa cacgcaaatc aaacgaagcg
      601 gacccgtacc cttgaaatta taaagtacaa gtatttccac tagttgtttg taagcgtaca
      661 gcctgaaccg tctggtcaga atctgacgaa ttagacaaag tggtgtccac caaataagta
      721 gtgggaac
//
LOCUS       DAZPAB010000478          725 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZPAB010000478 DAZPAB010000000
VERSION     DAZPAB010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.487"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            164..649
                     /locus_tag="ACHUPG_004465"
     CDS             164..649
                     /locus_tag="ACHUPG_004465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863755.1"
                     /translation="MRAIYLSVQQAWNGKITYSVSGESEFAKKFQGKALPFDVRIISA
                     SQNEDWLVIATKVLPGADLRTYVDFKNSTVHVDSAGLEKVAKCINCNNTLQVNIPHEA
                     GHVLGYLDDDYDSSSPYVGDISGLMNVGMELWERYLKNATITLNIIMPETKFTLLNVT
                     K"
     gene            646..>725
                     /locus_tag="ACHUPG_004466"
     CDS             646..>725
                     /locus_tag="ACHUPG_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863756.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAV"
BASE COUNT          235 a           93 c          155 g          242 t
ORIGIN      
        1 aacaaatatt gatggcaata tcattataca taaggatggt aggaggacgt atttattaaa
       61 tcaaaatact ggttcgatta cggctgtgat aagatggaag tatgagtagt ataaggataa
      121 taccgttaca gatgactgga ctatatggga aaaagctatt tttatgcgag cgatatattt
      181 atctgttcag caggcatgga atgggaaaat aacttattct gtttccggtg aatctgagtt
      241 cgctaaaaaa tttcagggta aagctttgcc ttttgatgta agaataattt ctgcatctca
      301 gaatgaagac tggttggtta ttgcaacaaa ggttttgcct ggtgctgatt taagaacata
      361 tgttgatttt aaaaacagta cggttcatgt tgattcagcg ggcctggaaa aagtagcgaa
      421 atgtattaac tgtaataata cgttgcaagt aaatatacct catgaagccg gacatgtact
      481 tggttactta gatgatgatt atgattcttc gtctccgtat gttggagata ttagtgggtt
      541 gatgaatgtg ggaatggagc tttgggaacg ataccttaaa aacgcaacta taacgttgaa
      601 tatcataatg cctgaaacta aatttacttt attgaatgta accaaatgaa atttttctgt
      661 attatagtga ttttcctttc aataaattac atagctatag tgaatgtttt caagtcttct
      721 gcagt
//
LOCUS       DAZPAB010000479          704 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZPAB010000479 DAZPAB010000000
VERSION     DAZPAB010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.488"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..443)
                     /locus_tag="ACHUPG_004467"
     CDS             complement(<1..443)
                     /locus_tag="ACHUPG_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFR0863757.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPD"
BASE COUNT          214 a          172 c          131 g          187 t
ORIGIN      
        1 tcatctggat aatcggcatg agcaataaca tgcttgccgg tgcgactgtg tacttcaaga
       61 ataaagggca aatgctgaca tttttccagc gccgttttcg cttgtttctg ttgattatct
      121 gccagcgcaa taaaccagtc gccgccattc atcaaccaca aagacatctg ctggaatgcc
      181 agcgcatcca tcgccatctg ttcatgattg cctcttaccg cacgaaccca atgttgttcc
      241 agtaactgca gacaacgtaa actttgcggc ccacgatcga taacgtctcc cactgagata
      301 agtaaatctc gccacggatc aaaacgacaa tgccataatt tgcggcgcaa ctgctcaaga
      361 caaccgtgta tatcgccaga aagccagata tgtcgccatt gatgacccgc aattctctga
      421 taaacgggcg caggctgttt catcaatatt ttcctcccgc gctaaagatc acataatctt
      481 aacaagaatg ttaaaaaacg ctggactcag acagtagagt gtgtgttatg gttgactata
      541 aagtcagcga aggaaatgct tctggctttt aacagataaa aagagaccga acatgattcc
      601 tgttttcgtc aacaaacaat aaaacccttt aaaattaatg cgttgaataa atttacgttc
      661 atcttttaat ccctgacacg taccattgca tattaataca ttca
//
LOCUS       DAZPAB010000480          701 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZPAB010000480 DAZPAB010000000
VERSION     DAZPAB010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.489"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..636
                     /locus_tag="ACHUPG_004468"
     CDS             <1..636
                     /locus_tag="ACHUPG_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR0863758.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT          216 a          144 c          179 g          162 t
ORIGIN      
        1 ccgttatcta aaacagaatt caatgcatta atggaaagtg aaaaaggaca gagccaaaac
       61 ttgtggaaat ttgccgttta ctccgggctt cgtcacgggg aactggcagc tctggcgtgg
      121 gaggatgtgg atttcgagaa gggaattgtg aatgtcagaa gaaacctgac gatacttgat
      181 atgttcggtc ccccaaaaac aaatgccggg atccggacgg tagcattact gcagccggct
      241 cttgaagcac tgaaggagca atacaaactg accgggcatc atcgcaaaag cgaaatcact
      301 ttttatcatc gggagtacgg cagaactgaa aaacaaaaac tgcattttgt tttcatgccc
      361 agggtgtgta acgaaaaaca gaaaccttat tactcggtaa gcagtttggg tacaagatgg
      421 aatgcagcag taaaacgtgc tggtattcgc cgccgtaatc cgtaccatac gcgacatact
      481 tttgcctgct ggctgttgac ggcaggagcg aacccggcat ttatagccag ccagatgggg
      541 catgaaactg cgcaaatggt gtatgaaatt tacggtatgt ggattgatga catgaacgac
      601 gaacagatag ccatgttgaa tgcgcggtta tcgtagttgc aaagtttgcc cccaatttgc
      661 cccatttagt accagagaac tgaaataatg caagaaaatc a
//
LOCUS       DAZPAB010000481          696 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZPAB010000481 DAZPAB010000000
VERSION     DAZPAB010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.490"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..673
                     /locus_tag="ACHUPG_004469"
     rRNA            <1..673
                     /locus_tag="ACHUPG_004469"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          175 a          164 c          206 g          151 t
ORIGIN      
        1 ttaagtcgac cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg
       61 cccgcacaag cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt
      121 cttgacatcc acggaagttt tcagagatga gaatgtgcct tcgggaaccg tgagacaggt
      181 gctgcatggc tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc
      241 aacccttatc ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata
      301 aactggagga aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca
      361 cgtgctacaa tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa
      421 gtgcgtcgta gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta
      481 atcgtggatc agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac
      541 accatgggag tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac
      601 tttgtgattc atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg
      661 gatcacctcc ttaccttaaa gaagcgttct ttgtag
//
LOCUS       DAZPAB010000482          685 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZPAB010000482 DAZPAB010000000
VERSION     DAZPAB010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.491"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(1..685)
                     /locus_tag="ACHUPG_004470"
                     /pseudo
     CDS             complement(1..685)
                     /locus_tag="ACHUPG_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
BASE COUNT          211 a           94 c          131 g          249 t
ORIGIN      
        1 aggtcatgtt tttaactttt atattcctct ggtttacaac ttcctctgtc ttgtaagtgt
       61 gtatattata tggggtatat gcttttttaa tagtatagtg aaacaattcg ttatccgcat
      121 ctattccgtc agagtgaact tcaaatgcac caggcgaaag aattgccagg ccagtattaa
      181 gcttaagtgc tggggaaaac aaattaaata acatgagctg catagtaggt caattgttct
      241 tcccagaaac atttaaaaaa tttaagtttt tgctctgttt tattatgggg aataaagttc
      301 caatgtcgga tgtattatta gtatcaatgt gtgttatttt attattgtcc agtcttaaac
      361 ttttgataga tggaagataa gtcaggaaat caagatttat atatttcaac tggttgttag
      421 cagcgtttaa tgtctgaaca ttgcggaagt tgctatagaa tattttaatt tcagaaagca
      481 aattgttgtt aataagtaac gtattcaagt tgggaaataa gacattgtgt aaacttgtta
      541 gtttattatg ggatagatcc agcagtaaga gttcattttt attacctgca acaatatcag
      601 ttaattgatt atggctgaga ttcaggtatt gcagccattc gcaagattct aattgcacaa
      661 actctagttg attatgtgcc gcact
//
LOCUS       DAZPAB010000483          685 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZPAB010000483 DAZPAB010000000
VERSION     DAZPAB010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.492"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            52..685
                     /locus_tag="ACHUPG_004471"
                     /pseudo
     CDS             52..685
                     /locus_tag="ACHUPG_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          205 a          172 c          173 g          135 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctcccccat taccccggaa
      241 caaattgaaa tccgtgagct caggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttga
//
LOCUS       DAZPAB010000484          682 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZPAB010000484 DAZPAB010000000
VERSION     DAZPAB010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.493"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1..525
                     /locus_tag="ACHUPG_004472"
                     /pseudo
     CDS             1..525
                     /locus_tag="ACHUPG_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            589..>682
                     /locus_tag="ACHUPG_004473"
     CDS             589..>682
                     /locus_tag="ACHUPG_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0863759.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATR"
BASE COUNT          190 a          139 c          165 g          188 t
ORIGIN      
        1 tgtgctgatc gtgcggatga ttactaccat gaccgtctgg cgctgactgt tgataattca
       61 acgatcgacg acaactacga acactacaca tataacggca cttacaataa tgccgctgat
      121 actcatgttg tagacgttta caacattggt acggctatta ctctggatca ggaagttgat
      181 ctgtccatca ctaataactc tcacgttgcg ggtattacgt tgactcaggg ttatgagtgg
      241 gaagatattg acgacaacac tgtcagcact ggcgtaaaca gcagcgaagt gtttaataac
      301 accatcactg ttaaagattc taccgtaacc tctggttcat ggactgatga aggtactact
      361 ggttggttcg gtaatacagg taatgccagc gattatagtg gtaaatctaa ctttgtcacc
      421 gtagatactg acggagatgg tgttgctgat agcactattg caagctggga tgatgttgca
      481 ttagctgtag ttgcacatcc gaatgttgat aatgctatgc agacctgaat cgccacgggt
      541 ttaacagaca cctcagagtc atttaagatg gcttaaagag aggtgcccat gagcggtaag
      601 cgttatcccg aagagtttaa aactgaagca gtcaaacagg ttgttgatcg cggttattct
      661 gttgccagcg ttgcaacacg tc
//
LOCUS       DAZPAB010000485          681 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZPAB010000485 DAZPAB010000000
VERSION     DAZPAB010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.494"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1..204
                     /locus_tag="ACHUPG_004474"
                     /pseudo
     CDS             1..204
                     /locus_tag="ACHUPG_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145474.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(270..635)
                     /gene="istB"
                     /locus_tag="ACHUPG_004475"
                     /pseudo
     CDS             complement(270..635)
                     /gene="istB"
                     /locus_tag="ACHUPG_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
BASE COUNT          171 a          156 c          170 g          184 t
ORIGIN      
        1 atgacaggcg aaaatcgtgg ccggggaagg ccgcgaaaga cagaaagtac ctatgcagat
       61 actcgtaatg atctgatccg cagtggcctt gaactgctga cgcagaatgg ttttcttgcg
      121 acgggagtcg atgccattgt taaaaatgcc aatgtcccta aaggctcatt ttattactac
      181 cttaagagta aagaggatta tgctgtcaac gacggatgaa aagtgatcca cttatatctc
      241 caccaacggc ccaatattga tccaccgttt tactcaggat tagcttctgc tataaccccg
      301 gcctttcgtt tctgtctgag tcgatagctt tctcctttga tttgaacgat atgtgagtgg
      361 tgtaagatac ggtccagcat cgctgaggtc agtgctgcat caccggcgaa cgtttgatcc
      421 cactgcccga acggcagatt ggatgtcagg atcattgcgc tcttttcgta acgtttagcg
      481 atgacctgga agaacagctt tgcttcttcc tgactgaacg gcagatagcc tatttcatca
      541 atgatgagca ggcggggggc cattactcca cgctgaagcg tcgttttata acggccctga
      601 cgttgtgccg tagataactg aagtaacaga tctgcgtaag cgtcaacgga gcaccgtatt
      661 gacgcttatt tattggtgag t
//
LOCUS       DAZPAB010000486          681 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZPAB010000486 DAZPAB010000000
VERSION     DAZPAB010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.495"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(125..255)
                     /locus_tag="ACHUPG_004476"
                     /pseudo
     CDS             complement(125..255)
                     /locus_tag="ACHUPG_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611212.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            253..336
                     /gene="tnpB"
                     /locus_tag="ACHUPG_004477"
                     /pseudo
     CDS             253..336
                     /gene="tnpB"
                     /locus_tag="ACHUPG_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          195 a          124 c          163 g          199 t
ORIGIN      
        1 tagtttgtgt aagcatatta cttccttatg ttatattttg gtaaaaaatt tattttttta
       61 cattatagtc ggctaaatga agtttactgt aaaaaaacga tactttttcg ggtggcgctt
      121 ccaatcagta gaatatgtgt ttttcaatga aggagccgat gacttttcgt ggatttccac
      181 ggaacgcgag aagcagattg ttttacgtgc cagccgcttg atgcgggtgc ggagggtcag
      241 gttgttacgt tcaagatata gcatgtgctg gatgaagatc ccttttctgg tcacctgttt
      301 atctttcgtg gccgacgggg tgacactgtt aaaatgtaat gaacgcatcc cacacaatta
      361 aacacggagt agtatgatgg ttaaaagggt gaaactggca ggtgtggagc ctcgcagctt
      421 tccggcatca tactgcccaa tacgggatgc ataaagcgtc ctcagactgg agactccaca
      481 aatgaaatat acaccggttg gcgttgatat ggcaaaacat gtcattcaga ttcacttcat
      541 caatgagcac atgtcagcgc cagtgatata agacggtaat ggggatgcgg acgatttcct
      601 ggactctcag aagatgaaac atgtttactg aagaacaaaa aattcgcgct attgagcttt
      661 atttcaaata tggtaagaag c
//
LOCUS       DAZPAB010000487          669 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZPAB010000487 DAZPAB010000000
VERSION     DAZPAB010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.496"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..417)
                     /locus_tag="ACHUPG_004478"
     CDS             complement(<1..417)
                     /locus_tag="ACHUPG_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFR0863760.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQ"
     gene            complement(427..>669)
                     /locus_tag="ACHUPG_004479"
     CDS             complement(427..>669)
                     /locus_tag="ACHUPG_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFR0863761.1"
                     /translation="GALQGQANAGQTYANNMSSIAQQSAALAAANANKPSSLQTAISG
                     GTSGAIAGAGLASLLGTSTPWGAGIGAGIGLLGSLF"
BASE COUNT          161 a          194 c          157 g          157 t
ORIGIN      
        1 ttgcattgct tctggagacg atgacgcaag gcgtgcgcca gccgctaagg tgccgataga
       61 attacgctgc tcttcatcaa tgaatcccat gcctttacga acggattcaa tctggtctgg
      121 atattgagta gccaactgac gcaaagcacc gcgatcacca gacgcataag cattagcgta
      181 cgcctgctga aattctttct gccgctgagc ctgcttttcc tgctgaaaaa cacccgcaat
      241 acctgaaaga ccttgcaaag cagtcagccc aacattgtta gcgcctgaac gctcaatatc
      301 attgttctgc ctgataagct gaagcgtatt gccgatgtca tttacgctcg gagcgtttga
      361 gttgacgccg ccgataccag ccaacaatcc gccgtttgtt ccttgccaag tagccatgat
      421 tcccccttaa aacaacgagc caagcaatcc gataccagca ccaataccag cgccccaagg
      481 cgttgatgtt cccaaaaggc tggcaagacc tgcaccggca atcgcaccag acgtgccacc
      541 gctaattgca gtctgaagac ttgatggttt attggcatta gcagcggcaa gagctgcgct
      601 ttgctgtgca atgctgctca tgttgttggc gtacgtctgc ccggcgtttg cctgaccttg
      661 cagcgcacc
//
LOCUS       DAZPAB010000488          668 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZPAB010000488 DAZPAB010000000
VERSION     DAZPAB010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.497"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>668
                     /locus_tag="ACHUPG_004480"
     CDS             <1..>668
                     /locus_tag="ACHUPG_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HFR0863762.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPV
                     LTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNK
                     ALKR"
BASE COUNT          133 a          230 c          177 g          128 t
ORIGIN      
        1 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
       61 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      121 accgtatccg cggccttctg acccagatac atccggcacc ggagcgcgtt ctcggtccga
      181 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      241 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      301 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      361 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatcacg ctgcgtaagc
      421 aaagagacga ggtggcgctt gaggtagaac agcgagttct tgctcaccct ctttacccgg
      481 tcctgaccag tatgcccgga gtcggtgtca ggaccgcagc cagactcctc accgaggtcg
      541 cctgccgcgc cttcgcctct gccgcacatc tcgctgctta tgctggcctt gcgccggtaa
      601 ctcggcgatc cggctcgtca atacgcggtg agcatccctc gcgacggggt aataaagctc
      661 tcaaacgg
//
LOCUS       DAZPAB010000489          665 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZPAB010000489 DAZPAB010000000
VERSION     DAZPAB010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.498"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..83)
                     /locus_tag="ACHUPG_004481"
     CDS             complement(<1..83)
                     /locus_tag="ACHUPG_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFR0863763.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQW"
BASE COUNT          194 a          167 c          136 g          168 t
ORIGIN      
        1 aaccactgca aaaaccagta gccggcactg ccggcggagg tgccgtaggc aatgcctgtt
       61 gatatttttt ccatctgata catatcccgc ccccccgaca gacctgtgct atcggaaaga
      121 aaaaaggcca tcagcaaaac tctgatggcc tgaatcacct ttaccaatat tgtatgaaaa
      181 aacacgcacg actaattgac aataattttc atttccatta aaaaagatcg cgtagcattc
      241 ttaattcatg aggaacttta cccgccagca atctgagtag cgaaagctgt tcatccccaa
      301 tgtttttgct ggcgggtcct tttttctacg gtcctcctcc ccggaggggg gcatttcatt
      361 atttttctaa tcgttactgg tgagaaggcg gaagaaaaag ccagttcttc gcccctaaat
      421 aacagcatga ttccagtcca tgcaaaatct gatttccagc tcacacatac cgagcgctgt
      481 acaaaaaacc gccagccaag gcagttaaga gtgtgttccc ggggtttgct tagaatattt
      541 tgtaagttgt ccgaagagat atttacaaca ccagaatgat gaatcatcag tcccctgcca
      601 gaggttcact acgtaacctg accgacaatg caccattctg gtgctgaaaa aagagcactg
      661 gaact
//
LOCUS       DAZPAB010000490          660 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZPAB010000490 DAZPAB010000000
VERSION     DAZPAB010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.499"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>660
                     /locus_tag="ACHUPG_004482"
     CDS             <1..>660
                     /locus_tag="ACHUPG_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /protein_id="HFR0863764.1"
                     /translation="QLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVT
                     VRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQ
                     RNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLT
                     LWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQ
                     A"
BASE COUNT          154 a          168 c          186 g          152 t
ORIGIN      
        1 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
       61 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct
      121 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
      181 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
      241 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
      301 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
      361 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
      421 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
      481 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
      541 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
      601 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta ggcaggctta
//
LOCUS       DAZPAB010000491          659 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZPAB010000491 DAZPAB010000000
VERSION     DAZPAB010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.500"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            103..>659
                     /locus_tag="ACHUPG_004483"
     CDS             103..>659
                     /locus_tag="ACHUPG_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0863765.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDS"
BASE COUNT          236 a          121 c          110 g          192 t
ORIGIN      
        1 agcaatacct ggagaaagag tacaaagcaa actgtattaa acttaataag ttatattgta
       61 tttcactaga gttcttatcg ttgataagaa attctggtta taatgattaa atcaacgaat
      121 atacaggtaa tcggttctgg tattatgcat caaataaaca acatacactc gttaactcta
      181 ttttctttgc ctgtgagttt gagtccatct tgtaatgaat attatttaaa agtctggagt
      241 gaatgggaaa agaacggtac cccaggcgag caacgcaata tcgccttcaa taggctaaaa
      301 atatgtttgc aaaatcaagg ggcggaatta aatctatctg agttagattt aaaaacatta
      361 cctgatttac cgcctcagat aacaacactg gaaataagaa aaaacctatt aacatatctc
      421 cctgatttcc caccaatgct taaggtaata catgctcaat ttaatcaact ggaaagctta
      481 cctgccttac ccgagacgtt agaagagctt aatgtgggtg ataacaagat aaaagaatta
      541 ccatatcttc ctgaaactct aactcattta cggatccata ataaccgatt gcatattctg
      601 ccactattgc caccggagct aaaattactg atagtttctg gaaacagatt agacagcat
//
LOCUS       DAZPAB010000492          656 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZPAB010000492 DAZPAB010000000
VERSION     DAZPAB010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.501"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>656
                     /locus_tag="ACHUPG_004484"
     CDS             <1..>656
                     /locus_tag="ACHUPG_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863766.1"
                     /translation="VLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRNLPSGVVP
                     AVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHR
                     YGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELS
                     WWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPL
                     P"
BASE COUNT          144 a          179 c          189 g          144 t
ORIGIN      
        1 gttctggttc cggaactcgg gctggtggtc cttaagcccg gtcgtgaatc catgccggta
       61 ttccacaata cccgggtact ggtggagccg gaaccgaaaa gcatgcgtaa tctgccgtcc
      121 ggggtcgttc ctgccgttcg ccagccgcta gtggaagaca aaacattgct gccgtttttc
      181 agtaacgcac gggtgattcg tgctgctggt ggtgctggcg cattgtctga ctggctgttg
      241 cgccatatta aatcctgcca gtggccacac ggcgattatc atcacagcga aaccgtcatt
      301 caccgttatg gtaccggcgc aatggtattg tgctggcact gcgacaacca gctgcgtgac
      361 cagacatccg aatcactcga gcaacttgct catcaaaacc tgtcagcatg gatgattgac
      421 gtcatcggtc acgcaataag cggtacgcag gagcgtgaat tatctctggc tgaattatcc
      481 tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg aagcggtatt acgtcgttcg
      541 ctggggttgc gtgcggaaaa aatccgctca atgtaccgtg aaagcgacat cgtgccggga
      601 gagcagaccg ccaccagcat actgaagcag cgcacaaaaa atcttgcgcc gctgcc
//
LOCUS       DAZPAB010000493          656 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZPAB010000493 DAZPAB010000000
VERSION     DAZPAB010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.502"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(151..>656)
                     /locus_tag="ACHUPG_004485"
     CDS             complement(151..>656)
                     /locus_tag="ACHUPG_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR0863767.1"
                     /translation="PVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDL
                     KELRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDP
                     STPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKL
                     VERLMQL"
BASE COUNT          173 a          160 c          144 g          179 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc gtaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttgccgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaa
//
LOCUS       DAZPAB010000494          637 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZPAB010000494 DAZPAB010000000
VERSION     DAZPAB010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.503"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            14..>637
                     /locus_tag="ACHUPG_004486"
     CDS             14..>637
                     /locus_tag="ACHUPG_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFR0863768.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQD
                     NAQETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDLKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIK"
BASE COUNT          188 a          166 c          148 g          135 t
ORIGIN      
        1 aggttaaaaa acaatgagca caaaaccact cttcctgtta cggaaagcga aaaaatcatc
       61 cggtgaacct gacgtcgtcc tgtgggcaag cgacgatttt gaatcgacca gcaccactct
      121 ggactatctg ctcgttaagt caggtaaaaa actgagcaac tattttaaag ctgttgccac
      181 aaattttcct gtcgttaatg accttccccc tgaaggtgag atcgatttta cctggagtga
      241 acgctatcaa ctcagcaaag actccatgac atgggaacta aaaccgggag cagcgccaga
      301 cgacgttcac caccaggata atgctcaaga aaccaaagaa ctggcgggag gccaggaaga
      361 aaacgcgcag gcagacgccc acgaggattg ccaggattgc gaagtctctg tagccacttt
      421 gcggttcaca cagcgtcttc tgcacatttt tacgtatgct gccggggatc tgaaatacct
      481 gcatcatgcc acccgtgaac aacgcaaaca cattactgct cttgagatgg atcaggaaaa
      541 cagctatgtc cagaatctgc tgttggccat acgcggcatg gcagaaccga caactctgga
      601 taatgccgcc ctgctccgcc tgactgatgc aattaag
//
LOCUS       DAZPAB010000495          636 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZPAB010000495 DAZPAB010000000
VERSION     DAZPAB010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.504"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            88..153
                     /locus_tag="ACHUPG_004487"
                     /pseudo
     CDS             88..153
                     /locus_tag="ACHUPG_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            381..629
                     /locus_tag="ACHUPG_004488"
     CDS             381..629
                     /locus_tag="ACHUPG_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR0863769.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          202 a          131 c          147 g          156 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacac
//
LOCUS       DAZPAB010000496          635 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZPAB010000496 DAZPAB010000000
VERSION     DAZPAB010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.505"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            18..491
                     /locus_tag="ACHUPG_004489"
     CDS             18..491
                     /locus_tag="ACHUPG_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0863770.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          210 a          113 c          127 g          185 t
ORIGIN      
        1 gagatgaacc ctatgcaatg tcctggttaa tatctcagtg tgctcatcag tgcactgata
       61 ataaaaagac ggagacagat gccatttacg ataaagttcg gagttcttat ttgctttcat
      121 gtattctgaa aaaaaacaaa aatgttgggc ttattcttca tgcaccatca tttgtttctg
      181 tgtctgaaaa aatcgccaga attgtgatgg caaattactc ccgtaactgg agtaattccg
      241 agttggccag tgcggtttta atgagtgaat cttctttaaa aagaagaatg tacaaagagg
      301 tcggttctat aagtacattt gtacataaaa taaagcttac agaagctatc aggaaattac
      361 gaaggacaaa tactcccatt tctgtcatat catctgagtt aggttacagt tcaccatcgt
      421 atttttctaa agtcttcttt aaatatttaa agacataccc tcaaaacata aggaagaaaa
      481 atggtcggta atgtcaccaa ttaggaggac ttgcagagcc gcttttttac gatactgaga
      541 tcaaacagac ataatctatg cggagattta cagcagggca tggttcacaa atctgacagc
      601 gatgaattat cggcactgcg ggcagaaaat gctcg
//
LOCUS       DAZPAB010000497          633 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZPAB010000497 DAZPAB010000000
VERSION     DAZPAB010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.506"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            58..633
                     /locus_tag="ACHUPG_004490"
                     /pseudo
     CDS             58..633
                     /locus_tag="ACHUPG_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          223 a          107 c           95 g          208 t
ORIGIN      
        1 ttttcacccg atcattttaa taatgatttt tattgattat ttataaagga taaaataatg
       61 ttccctgttt catcaattgg taatgatatt agtagtgatt tagtccgtcg aaaaatgaat
      121 gaccttcctg aaagccccat aggaaataac ctcgaagcac tagcaccagg tatagaaaaa
      181 ctaaaacaga cctctattca aatggtcact ttacttaacg cgttacaacc aagtggaaaa
      241 tgcattatca ctggtgattt tcaaaaagaa ttagcgtact tacaaaatgt aattctttat
      301 aatgactcgt ctcttcgtct ggatttttta ggttataaca cccaaattat tcaacgatcg
      361 gacaatacct gcgaacttac cattaatgaa ccgttaaaaa actaggaaat atctacaggc
      421 aatatcaatg ttaattgccc attaaaagat atttacaatg aaatcaggag gttgaacgta
      481 atctttagtt gtgggactgg agatatagtt gatctatcct ctctggactt acgtaatgtc
      541 gatttagatt attatgattt cacagataaa catatggcta atactattct aaatcatttt
      601 aaattggatt caacaaattt tactaatgcc aac
//
LOCUS       DAZPAB010000498          623 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZPAB010000498 DAZPAB010000000
VERSION     DAZPAB010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.507"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..251)
                     /locus_tag="ACHUPG_004491"
     CDS             complement(<1..251)
                     /locus_tag="ACHUPG_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFR0863771.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
     gene            complement(272..>623)
                     /locus_tag="ACHUPG_004492"
     CDS             complement(272..>623)
                     /locus_tag="ACHUPG_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0863772.1"
                     /translation="TVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRH
                     RKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNRR
                     LSESVPCMPHKKAG"
BASE COUNT          141 a          145 c          141 g          196 t
ORIGIN      
        1 ctttgcaggt aagtcagtaa tgtttcttgc tgatcgcgcc agacgttctg cacatcaaca
       61 ttttcattac tgagcagtat tttaactttg ctgacctgca cgccgttgtc gatccagcgt
      121 ttgatctcgc ggatccggtc aatatcggca tcgttgaaca gccgatgacc gccgtctgtc
      181 cgttgcggtt tcagcaatcc gtaacgcctc tgccacgcgc gtaacgtgac agggattaaa
      241 ggtaaaccaa ttgattttgc aagtttttat tttaccccgc tttcttatgg ggcatgcatg
      301 ggacactttc agatagtctt ctgttaagaa gttctatctg ttcgtgattg ttgtctttca
      361 tccatgctcc gtaaacattg aataccattt gtgcgtttgt gtggcccatc tggcttgcga
      421 taaaactagg attagctcct gcggcaagtg accagcatgc ataggtatgc ctggattgat
      481 acgattttct gtgtctcaga cctgcgcgtt ttaagatact tgtccatgac tccctgatgg
      541 agtcaacctt atagtgcggt ccggaaaact gccgctgttt tattacctga ggactaaaaa
      601 caaaagtgca tttatgcaca gta
//
LOCUS       DAZPAB010000499          620 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZPAB010000499 DAZPAB010000000
VERSION     DAZPAB010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.508"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1..620
                     /locus_tag="ACHUPG_004493"
                     /pseudo
     CDS             1..620
                     /locus_tag="ACHUPG_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
BASE COUNT          163 a          151 c          165 g          141 t
ORIGIN      
        1 ggtggtcaac aggagtgtga aatgaggcgc ttcgcaggcg catgtcgttt cgttttcaat
       61 cgtgctctgg cacgtcagaa tgagaatcat aaggccggta ataaatacat cccttacggg
      121 aaaatggctt cctggctggt tgagtggaaa aatgccactg aaacgcaatg gcttaaagat
      181 tctccctcac agccattgca acagtcactg aaagaccttg agcgggctta caaaaacttc
      241 ttccggaagc gggctgcttt tccccgattc aaaaagcggt gacagaatga tgcattccgc
      301 tacccgcagg gcgttaagct cgatcaggaa aacagccgta tttttctgcc gaaactgggc
      361 tggatgcgct accggaatag ccgtcaggtc acgggtgttg tgaaaaatgt cactgtcagc
      421 cagtcctgcg gtacgtggta catcagtatt cagacagaaa gtgaagtatc cactccggct
      481 cacccttcag catcaatggt cgggctggat gctggcgtgg ctaaactcgc cacgctgtca
      541 gatggcacag tctttgagcc tgtaaacagt tttcagaaaa accagaagac gctggcgaga
      601 cttcagcgac agttaagccg
//
LOCUS       DAZPAB010000500          619 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZPAB010000500 DAZPAB010000000
VERSION     DAZPAB010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.509"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..>619)
                     /gene="ipaH3"
                     /locus_tag="ACHUPG_004494"
     CDS             complement(<1..>619)
                     /gene="ipaH3"
                     /locus_tag="ACHUPG_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFR0863773.1"
                     /translation="RNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQ
                     NALISLPELPASLEYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINA
                     DNNQLTMLPELPTSLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHS
                     EETEIFFRCRENRITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          164 a          106 c          168 g          181 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 cgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgat
//
LOCUS       DAZPAB010000501          613 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZPAB010000501 DAZPAB010000000
VERSION     DAZPAB010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.510"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            15..613
                     /locus_tag="ACHUPG_004495"
                     /pseudo
     CDS             15..613
                     /locus_tag="ACHUPG_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816255.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
BASE COUNT          177 a          111 c          136 g          189 t
ORIGIN      
        1 gagaagcagt cgatattcat agctcacttg agctttcatt tatattgcat aaatatcatt
       61 tgcgtgtaga tgagaagaaa tctcgaaatg tattcagcgc catacgtgat gagctcatcc
      121 ttgatgggga ttcacgacaa aacttagcta ctttctgtca aacatggctt gatgatgaaa
      181 tccgtgagtt gcttaattta gctattgata aaaacatgat ttataaagat gaatatccac
      241 aaatggcgga acctgaaggt cgctgtgtgc gtatgctttc cgattcgtgg agtttccccg
      301 attcgcgaca caccttaggt tgctcaacca ttggttcatc tgaagctgct atgctcggcc
      361 gcttggcgtt aaaatggcag tggtgtaaaa aacgcgaagt acaaggtaaa tcaaccgaac
      421 ctgatttgcg atcctgtgca aatatgctgg cataaattct gccgttactg tgatgtgaaa
      481 ttgtgtgaaa ttccacttga tgatcatcac ctgataatga atgctaaaga ggttttgaag
      541 catgtcgatg aaaacactat tggtgtcgtt acgactttag gtattactta tttttgtaaa
      601 gcagattgcg tta
//
LOCUS       DAZPAB010000502          605 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZPAB010000502 DAZPAB010000000
VERSION     DAZPAB010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.511"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(29..397)
                     /locus_tag="ACHUPG_004496"
                     /pseudo
     CDS             complement(29..397)
                     /locus_tag="ACHUPG_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          175 a          109 c          139 g          182 t
ORIGIN      
        1 ttgtctggag attcaggggg ccagtctaat cagttgtcat gcactcggaa taaatcatac
       61 cgtgaatagt gctgtttttg attgtcagac tattggcgtt ggtattggta gaaataccgt
      121 cgtccagata gtaggtagaa atcacgccgt taacggtagc gttattaatg accgggtaga
      181 tatcgccgtt gtaatagcta tcagcggcat agttgttcca gcctacataa ccatcatagt
      241 aaacgtcatc agcataggtg gcgtcgttgt agtgatggaa agtattgtaa gtttgaccag
      301 aaatatcgtt ggtcgctgca ttagcctgag aggtgatagc cagagtgcaa gccaatgcta
      361 attgtgatac tacaagtttc tttttccagg agtgcatttg tcatccctcc tcagggacgt
      421 aataagttga tccatcaatt atcaatgcat agaaaacata ttatgtttcg cgctaacgat
      481 tatgctgcgt tacagacgaa tggttcaatt attctttccc ctaagtccga atgtggacat
      541 atataattta tggagaaaaa cacgcatcaa attgataaaa ggacgatatt tcagtatgtt
      601 gcaga
//
LOCUS       DAZPAB010000503          585 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZPAB010000503 DAZPAB010000000
VERSION     DAZPAB010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.512"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>585
                     /gene="gtrII"
                     /locus_tag="ACHUPG_004497"
     CDS             <1..>585
                     /gene="gtrII"
                     /locus_tag="ACHUPG_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HFR0863774.1"
                     /translation="QTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICR
                     SLKLESYELLIGMLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVI
                     FSGIVLSILSMAIYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALST
                     LIYLLLTKAIKHYFSLESNEYISNYIQNASDIKWL"
BASE COUNT          174 a          113 c           81 g          217 t
ORIGIN      
        1 tcaaacaatc acgcttgggc ggtggtttca cacttttttg cgacattact ttctccctga
       61 gcctttttca ctttatataa caccattaat agccttatct tttatcatca tttcagcatt
      121 tataatctgc agatcgctaa agctagaatc ttatgaatta ttgataggta tgttagtatt
      181 tattaccttc cctcagatct cctatcaatt agagtttctt aaccaagctg atactgtggg
      241 aattgctttt ctactggcag cgatatcagc aattattttt cactcgcaaa aaaataggat
      301 tgtgatattt tctggtatag tactgtccat tctttcaatg gcaatctacc aaacattcgt
      361 aacatatatt attgcattcg tcattgggtt gcagataaat tcgataatac gaaatgagaa
      421 aaatattcgt gaatcttttt atagttcatg tttatctcta tccctcatag ctttatctac
      481 cttaatttac ctgctattaa ccaaagctat caagcattat ttttcgcttg aatcgaacga
      541 gtacatctca aattatatac aaaatgcaag cgatattaaa tggct
//
LOCUS       DAZPAB010000504          585 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZPAB010000504 DAZPAB010000000
VERSION     DAZPAB010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.513"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..574
                     /locus_tag="ACHUPG_004498"
     CDS             <1..574
                     /locus_tag="ACHUPG_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0863775.1"
                     /translation="AEEDPASATRPFRTEVKRSRLLIDEYLLIRKIADVQNEWFGLCM
                     DLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPTTISRLNLTLADVLDN
                     LKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFHEIRSLSGRLHSAEKG
                     SDFTQALLGHRSSSMTDKYRDGRGREWKDI"
BASE COUNT          191 a          124 c          148 g          122 t
ORIGIN      
        1 tgccgaagaa gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg
       61 actgttaatt gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt
      121 cggcctttgc atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat
      181 gagatgggag gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa
      241 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      301 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      361 gacaattgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      421 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      481 cagtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      541 tgacggacga gggagggaat ggaaagacat ttagaattaa tttcg
//
LOCUS       DAZPAB010000505          584 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZPAB010000505 DAZPAB010000000
VERSION     DAZPAB010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.514"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            124..504
                     /locus_tag="ACHUPG_004499"
     CDS             124..504
                     /locus_tag="ACHUPG_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFR0863776.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT          189 a          107 c          125 g          163 t
ORIGIN      
        1 gatttttacg aatattcaga ttatcataaa aaatgcaaca gacaggaatg caataatttt
       61 gcattatctc gttgcttgta atgcaaatat gcattattgt cagtgtgtga caataacaga
      121 gagatgacga tggcgaccat taacgcacgt attgatgatg atataaagaa ccaagcagat
      181 gaagtgctga agctgatgaa tatcagtcaa actcaggcta ttgcagcttt ttatcagtat
      241 attactgaac aaaagaaact gccatttgtg atcacctcaa ttgtaaaaac gccgcatgat
      301 ttattacgtg aaagtacaga catgttagct gaagctcttg cagtgatttc caatctacag
      361 gtgtggactg aacagcagga tgggattgga aaagcaaagc taatggagta ttaccgccgc
      421 ctcgacgcac tgtactgttg tgcaaaagag aaaataggtc ttctttcaga taaccgggat
      481 gctgaactag gctgtgtccc ttaatcatct gagttatagt aactgtctgt ttacacaaca
      541 tattgaacta tggctcgcta cgaccttccc gatgaggcat ggac
//
LOCUS       DAZPAB010000506          582 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZPAB010000506 DAZPAB010000000
VERSION     DAZPAB010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.515"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(141..>582)
                     /locus_tag="ACHUPG_004500"
     CDS             complement(141..>582)
                     /locus_tag="ACHUPG_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR0863777.1"
                     /translation="GGKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLR
                     DMINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELL
                     NDAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          148 a          163 c           99 g          172 t
ORIGIN      
        1 caccagcacg tttaacgtcc tgtgccgttt ttcaggtata aaaaaaaccg cataaagcgg
       61 gctctttcaa atgtccatgt ctgctattcg cctcgcggta cagctttgcg aagcgtacaa
      121 gaattgaagc agtttttacg tcaaaaagca ataacttttt tctctatacc aaaagccata
      181 accattggtt tgtacaaaat aaattctgcc acctttagcc aatgctcaat gcgtctttca
      241 caggttctta aactccattt cgggtgtgca tcattcagca gttcagccat tttgcatttg
      301 gtcatccccc gcccctcata ccgttgccga aggacgctaa tcaatcctgg atgctctgcc
      361 agcacctcac ttatgacccg atcaatacat aacgcctctg catcagtaca atgcgccagc
      421 cagctctttt gcttaccgtt aatcatatcc cgcaaaaaag cctcaagttc agacttgttc
      481 agacctgctt ttttcatcct ccggagcgcc tcgttaattg ccgtttttgt cagcttttta
      541 gaggtcaaca tcaggttaaa catattcccc gtcttaccgc ca
//
LOCUS       DAZPAB010000507          580 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZPAB010000507 DAZPAB010000000
VERSION     DAZPAB010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.516"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            133..>580
                     /locus_tag="ACHUPG_004501"
     CDS             133..>580
                     /locus_tag="ACHUPG_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0863778.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQG"
BASE COUNT          221 a          123 c           76 g          160 t
ORIGIN      
        1 aaaataatag cctaaaaaaa tgatatttct gatagttata aactgaaatt tcttaatctt
       61 gattataaca agctagcatc gctggatgtc tcccggcaag aatctctgat tgagttaagt
      121 gcccatcaca atatgatcac tgaccttata ttacacaatc atcccaggat gaaaacaatc
      181 actttaaacg acaaccatat tgcacattta aacgccaaaa acactacaaa actggaatat
      241 ttaaacttaa gcaataacaa tttactgcca accaatgaca ttgatcaact aatatcatca
      301 aaacatcttt ggcatgtatt agttaacggc atcaacaatg atccacttgc acaaatgcag
      361 tactggactg cagtaagaaa tataattgat gacactaatg aagtgaccat tgatttatca
      421 ggacttaatt taaccactca accaccaggg ctgcaaaact tcacctctat caatcttgat
      481 aataaccaac tcacacattt tgatgcaacc aactacgata gactcgtaaa gctaagtctg
      541 aatagtaatg ctcttgagtc aataaatttt cctcaaggca
//
LOCUS       DAZPAB010000508          579 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZPAB010000508 DAZPAB010000000
VERSION     DAZPAB010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.517"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..318
                     /locus_tag="ACHUPG_004502"
     CDS             <1..318
                     /locus_tag="ACHUPG_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863779.1"
                     /translation="FSQRNPDNSIVFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGH
                     EMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNV
                     ASI"
     gene            321..579
                     /locus_tag="ACHUPG_004503"
                     /pseudo
     CDS             321..579
                     /locus_tag="ACHUPG_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          177 a          122 c          146 g          134 t
ORIGIN      
        1 ttcagccaga gaaacccgga taacagcata gtgttcactg ttaacggaca ggtaattccg
       61 ttaaattacg gaaacttcga tgcccgctat aaatatcgaa cagagggggt acaggatgta
      121 cggtatggcc atgaaatgta ttacagcccc ggcagtaaca ccgtttcgtg gagattttgc
      181 gcaccttcgg gacacgggct gtcagggatg gcgatatcgg ataccggccg taactcagcg
      241 gataacgttg acggtgtgta ttaccgacca ctgcaaaaac tgattaatgg cacctggtat
      301 aacgtagcga gtatttaaca atgttgcatt taaaaaatat tactgcgggt aatccgaaaa
      361 cagcagaaca atatcagatg acaaaacgat attcggtcac ctggcttttt tcagaagacg
      421 gaaaaaactg gtatgaagag ctgaagaatt tcgccaggac acaattaaaa taacttacac
      481 cggagacggt cgcgtggtgt gggtcggtaa ggatgtgaca ggcattgaac cccgtaacgc
      541 cagtgttatt gaagttcctg atattaccgc caaccgccg
//
LOCUS       DAZPAB010000509          572 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZPAB010000509 DAZPAB010000000
VERSION     DAZPAB010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.518"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(104..362)
                     /locus_tag="ACHUPG_004504"
                     /pseudo
     CDS             complement(104..362)
                     /locus_tag="ACHUPG_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            346..572
                     /locus_tag="ACHUPG_004505"
                     /pseudo
     CDS             346..572
                     /locus_tag="ACHUPG_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          152 a          125 c          155 g          140 t
ORIGIN      
        1 gaaaaactat ttggtaaaat ccaacaagag tcgagaggtt cgcgttgctt cagcagggcc
       61 tcagcctgtt caaccagact cagttgcagg tcgtgacgca gatttagtgg tggtggttga
      121 ttgtcagata ccagccgatg atcttgtagt gcatatcatc agactttgag aagcatatgg
      181 ttttgcgggt aagccgctta agatgtgtcc gcaggttcag attatgtcgc tctatccgct
      241 gagtgcattt cttgcttata acatgactgg ttgaggacaa taaagttcgg tataccggct
      301 caggcatcgg tcatgtagaa ggcaatgcta aactggctca gcaaactaag agcagcgcgc
      361 atccacttac ccaatgcggt tgagaaaata gccttcgatc gctttcatgt ggcgaaacaa
      421 ccgggtgagg tggtggataa aacccgccag aatgaacatc cgcatcttcc tgttgaaagt
      481 cgtcgtcagg caaagggaac tcgcttcctg tggcagcaca gcgataaatg gatgaccgaa
      541 tccaggcagg aaaagctgat atggctgcgt gc
//
LOCUS       DAZPAB010000510          570 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZPAB010000510 DAZPAB010000000
VERSION     DAZPAB010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.519"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
BASE COUNT          174 a          111 c          102 g          183 t
ORIGIN      
        1 cttctgggga acgctgaaaa atgaaagcct gagccactat cgttttaaga gccgggatat
       61 ttcgtcagca tacgggaaaa ctgattgagg tcggcctcag ttttgaggcc tttagccagt
      121 tcagccgcaa gggctttaag cttcttttcg tccataattt acctgtctcc gttgttggag
      181 taaacatatc aaaaacaggc aattacacaa tttaaattac agtctcaaaa caaacccaac
      241 ggtcaatata gtttaccagc aagcttacta gcaaagtgct aatcatttgg tatctttcgt
      301 gctattctcc ttttacgtaa gtacttaagg ggaacgtatt tactactttt aaagaaagct
      361 ccgttactat ctgaaatgca atgagataat aaacgattac tctcggttag tatctcatga
      421 tcatttacag actttgcata atcgcgaata ctatataaca gagttttaac attacaggat
      481 gaatagtctc tcttatcaac tcctaataaa agaaggatta tggtcagtcg tttttgctgt
      541 cttatcatat attatttagt gcagttccta
//
LOCUS       DAZPAB010000511          566 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZPAB010000511 DAZPAB010000000
VERSION     DAZPAB010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.520"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..297
                     /gene="pliG"
                     /locus_tag="ACHUPG_004506"
     CDS             <1..297
                     /gene="pliG"
                     /locus_tag="ACHUPG_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR0863780.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="ACHUPG_004507"
     CDS             complement(405..>566)
                     /locus_tag="ACHUPG_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR0863781.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DAZPAB010000512          557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZPAB010000512 DAZPAB010000000
VERSION     DAZPAB010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.521"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..549
                     /gene="virK"
                     /locus_tag="ACHUPG_004508"
     CDS             <1..549
                     /gene="virK"
                     /locus_tag="ACHUPG_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR0863782.1"
                     /translation="PSGFDREGELMLSLFYNKTVIARLTFSVILTQNGHIAFIGGLQG
                     APKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNISECLAVSEHSHVFRQLRYW
                     YQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSDIASKKRSGYRKRYALLDY
                     IHETTIRSLDAYPVHSEHQNLN"
BASE COUNT          154 a          109 c          129 g          165 t
ORIGIN      
        1 cccagtggtt ttgaccggga aggagagttg atgttgtcat tgttttacaa taaaacagtt
       61 attgcccggt taacattttc agtcattctg actcaaaatg gacacattgc ttttattggc
      121 ggattacagg gagcccctaa aaataccgga cctgatgtta ttcggtgcgc aacccgggcc
      181 tgttatggga ttttcccaaa acggattatt tttgaagcat tttgtgcgtt gatgaaagct
      241 tgcaatatct ctgagtgtct ggcagtcagt gagcacagtc atgttttccg gcaattgaga
      301 tactggtatc agaaacgcaa aacctttgtt gcggtctata gtgatttctg ggagtctgta
      361 gcaggaaaaa cctgtggtga ctggtacaga ttaccaaccc aggtgattcg aaagccgcta
      421 agcgatatag ccagtaaaaa acgctctggg taccgaaaac gatacgcttt gctggattat
      481 atccatgaaa ctactatccg ctctttggat gcatatcctg tacactcaga acatcagaat
      541 ttaaattaag gtttgat
//
LOCUS       DAZPAB010000513          557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZPAB010000513 DAZPAB010000000
VERSION     DAZPAB010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.522"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>557
                     /locus_tag="ACHUPG_004509"
     CDS             <1..>557
                     /locus_tag="ACHUPG_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFR0863783.1"
                     /translation="APTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLHPK
                     IGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKA
                     TYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDT
                     ITRNHQCRSMWQLLKKVRHFMETVS"
BASE COUNT          164 a          137 c          148 g          108 t
ORIGIN      
        1 tgcgccaact ctgcgtatcc gtgacccgca taaagatgaa aagatggcag caatccataa
       61 agcactggac gaatgcagcg cagagcatcc ggtcttttat gaagatgaag tggatatcca
      121 tcttcatccc aaaatcggtg cggactggca actgcgcgga cagcaaaaac gggtggtcac
      181 gccgggacag aatgaaaaat attatctggc cggagcgctg cacagcggga caggtaaagt
      241 cagctatgtg ggcggcaaca gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct
      301 taaagcgaca taccgtcgtg cgaaaaccat cacgctgatc gtggacaact acattatcca
      361 caaaagccgg gaaacacaga gctggctgaa ggagaacccg aagttcaggg gcatttatca
      421 gccggtttac tcgccatggg tgaatcatgt tgaacggcta tggcaggcac ttcacgacac
      481 aataacgcgt aatcatcagt gccgctcaat gtggcaactg ttgaaaaaag ttcgccattt
      541 tatggaaacc gtcagcc
//
LOCUS       DAZPAB010000514          553 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZPAB010000514 DAZPAB010000000
VERSION     DAZPAB010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.523"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..>553)
                     /locus_tag="ACHUPG_004510"
     CDS             complement(<1..>553)
                     /locus_tag="ACHUPG_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HFR0863784.1"
                     /translation="VEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKN
                     TDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKT
                     ATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVREDVMDQ
                     IVQEGSLEYATDVIIHLQQIDIKW"
BASE COUNT          134 a          119 c          135 g          165 t
ORIGIN      
        1 ccatttaata tcaatctgtt gaaggtgtat tattacatca gtagcatatt ccagactgcc
       61 ttcctggacg atctgatcca taacatcctc acgcacttta tatcttgtgc atttcatttc
      121 aagtgccgtg agtacagcca ttactggtga tggtagctgt gttgttttca ggccgtcgat
      181 ctgactgatc gcttgggcga cggtgttttg ccattctaaa gaacaggtgc tgaagtcaaa
      241 tgtggctgtt ttattatccc gttgcgacac gctacaggct aagcgacgta attgggtcaa
      301 tttgtctgct tgctgaacag gaactccggg aaagcggcga tgacttaacg tggtgtgtac
      361 aacttcgggc ggaaaaacga cagcaatatc tttcgccagc caaggtaaaa cagcatctgt
      421 atttttgtca gccgcacggg tttctgctac gaaagagggt tgttcctgaa taatggtgga
      481 taagtccgct accttgagag agtccgttac cgtttgggaa ggttgcaaag aattatcttt
      541 cgcttgctcc aca
//
LOCUS       DAZPAB010000515          550 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZPAB010000515 DAZPAB010000000
VERSION     DAZPAB010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.524"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..143)
                     /gene="csgC"
                     /locus_tag="ACHUPG_004511"
     CDS             complement(<1..143)
                     /gene="csgC"
                     /locus_tag="ACHUPG_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HFR0863785.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLR"
     gene            complement(202..>550)
                     /gene="csgA"
                     /locus_tag="ACHUPG_004512"
     CDS             complement(202..>550)
                     /gene="csgA"
                     /locus_tag="ACHUPG_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFR0863786.1"
                     /translation="NNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGAD
                     VGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVT
                     QVGFGNNATAHQY"
BASE COUNT          146 a          127 c          132 g          145 t
ORIGIN      
        1 tcgcgcaggg acaatatttg tactctgcac agacaagatt gagtaagagt aacttcagga
       61 ataatggtgt acatatcccc ttgctgggtc gtattaaagg ttatctggct ggaaagtgcc
      121 gcaaggagta ataacgcatt catattcttc tcccgaaaaa aaacagggct tgcgccctgt
      181 ttctgtaata caaatgatgt attaatactg atgagcggtc gcgttgttac caaagccaac
      241 ctgagtcacg ttgacggagg agttcgatgc agtctggtca actgcagcac cgttgccacc
      301 accgaactgt ttaacagtca tttcagaatt tttgccgttc cactgatcaa gagtggcgct
      361 gttaccgaag ccacgttggg tcagatcgat tgagctgtca tctgagccct gaccaacatc
      421 tgcaccatta ccaccgccat gctgggtaat agtcaagtca gagttacggg catcagtttg
      481 cagagcaagt gcagagttac cgccaccgta ctggtaaatg ttcagctctg aatttgggcc
      541 gctattatta
//
LOCUS       DAZPAB010000516          537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZPAB010000516 DAZPAB010000000
VERSION     DAZPAB010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.525"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1..487
                     /locus_tag="ACHUPG_004513"
                     /pseudo
     CDS             1..487
                     /locus_tag="ACHUPG_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          124 a          118 c          136 g          159 t
ORIGIN      
        1 actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg aacaacagca
       61 acataatcaa gcggccgcga aaaaacgcat cccggttatc gaagctctgt tacgacatcc
      121 cggtgctttc ctgaagatta ttgcactacg actgtgcgag ttgctgacga tgtacattgt
      181 tactgccttt gcacttaatt attcaaccca gaatatgggg ttaccgcgcg aacttttcct
      241 taatattggt ttgctggtag gtggattaag ctgcctgaca attccctgtt ttgcctggct
      301 tgccgatcgt tttggtcggc gcagggttta tatcacaggc gcgttgatcg gaacgttgag
      361 cgcatttcct ttctttatgg cgcttgaagc acaatctatt ttctggatag ttttcttctc
      421 cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac cgatgtttac
      481 cgaaatgtga ggtagcctga gtttaacgga cactccttcc tgaaatagaa tggcatc
//
LOCUS       DAZPAB010000517          535 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZPAB010000517 DAZPAB010000000
VERSION     DAZPAB010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.526"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..309)
                     /locus_tag="ACHUPG_004514"
     CDS             complement(<1..309)
                     /locus_tag="ACHUPG_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphate phosphatase SopB"
                     /protein_id="HFR0863787.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     L"
BASE COUNT          147 a          115 c           84 g          189 t
ORIGIN      
        1 aagtttatag gtatctaatc gttccatcat ggctttaatt tttgacgtat caccacctgc
       61 caactttatt gcctcgcaaa aaacttcagc tctgtcacgc gcaaacaaaa ggctttttcc
      121 tgttaagctt gtttggttct gtaaatataa tcgattaagc gcttcgatcc ccttatttct
      181 ttctccattt ttaactcttt gatatgaaat cacgcttaca cctttgtgct ttccggtttc
      241 ttctgcgcct ttataggaat ctccgctttg aaatgaaacc tgatgcaatc ccaaattagt
      301 tatgtgcatt taattatcct cagtcataaa tggggcattg attgcagttt tgtacctcgt
      361 tttacaacaa aaaagatact tattcaacag ggttctctct ttttctataa cactccgagc
      421 ctgatacatc aggcacttta tcttgtggga tttcatgatg aaacgagcac tacatatgct
      481 cacgaggtac atattatgta cagcaaaaga aaatttgatt atgttaacag gctca
//
LOCUS       DAZPAB010000518          535 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZPAB010000518 DAZPAB010000000
VERSION     DAZPAB010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.527"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..89)
                     /locus_tag="ACHUPG_004515"
     CDS             complement(<1..89)
                     /locus_tag="ACHUPG_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0863788.1"
                     /translation="MSRKTQRYSTEFKAEAVKTVPENQLSISEG"
     gene            complement(427..>535)
                     /locus_tag="ACHUPG_004516"
     CDS             complement(427..>535)
                     /locus_tag="ACHUPG_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HFR0863789.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          138 a          124 c          110 g          163 t
ORIGIN      
        1 ccttcactga tcgaaagctg attttcagga accgttttga cggcttcggc tttaaactcc
       61 gtagagtaac gttgggtttt tctgctcatt attggctcct tctgatgcca ttctatttca
      121 ggaaggagtg tccgttaaac tcaggctacc tcactcccgt aaagacagga gtcaagttcc
      181 cgctccgaca taactgcgtg caatggattt tgaaggaacc gtcagcccat tccccggagg
      241 caaacatggg ctggcaaaag tgtagcggta ttaggcacag ctatttagga aagagggacc
      301 atctccatct cattaaatgg accacgccag acaattcact gtcatgctga tgctgcactg
      361 tggggacaga atcactcatg atgacttcga taatcgacga cattattatt tggccgagct
      421 tttaggtcat atctcaaatc gtttgcctaa tattgctcca ttttttaata aaaaatcaat
      481 catttttgaa tctttgcttt tcattgcatt gtcaagcata gtgtccccac tattt
//
LOCUS       DAZPAB010000519          529 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZPAB010000519 DAZPAB010000000
VERSION     DAZPAB010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.528"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            381..>529
                     /locus_tag="ACHUPG_004517"
     CDS             381..>529
                     /locus_tag="ACHUPG_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR0863790.1"
                     /translation="MSQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLRDIR
                     KMLEEL"
BASE COUNT          129 a          140 c          115 g          145 t
ORIGIN      
        1 tagggtctct ctataggtct tctctatagg tcttctctat ggtctctcta taggtctctc
       61 tagggtctcc tctaggcatt acccccagaa gctaactcta tggccctcta tggtcctcta
      121 tggccctgta cgtcattctt taggatgccc tgtagtttcg tatagcctga tgcctagaga
      181 gaccctagag agaccctaga gtggctccta gagagaccct cccagtagct ccgcacagct
      241 acatcgtcat catcatagta ttcaatctca aagtcattct gtttgcatcc tgttgtcctg
      301 ctggtctccc ttcgcttggg gactttgagt aggtcagtgg gggtgcgact tcagggtgca
      361 aacaaaaagg aaatcaagcg atgtctcaac aaccacaaca accacaacaa gaaatcagca
      421 tcattaacct cgaccagctt gtgtccatga cctcagttga aattgctgag ctgacaggca
      481 aggagcaccg aaatgtcctt cgtgatattc gtaagatgct cgaagaact
//
LOCUS       DAZPAB010000520          526 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZPAB010000520 DAZPAB010000000
VERSION     DAZPAB010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.529"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>526
                     /locus_tag="ACHUPG_004518"
     CDS             <1..>526
                     /locus_tag="ACHUPG_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863791.1"
                     /translation="FKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNACHKVA
                     IFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGFVSFV
                     NREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYMLDEK
                     VLSAIAEFMNEKGWR"
BASE COUNT          191 a           68 c           97 g          170 t
ORIGIN      
        1 tttaagtctt taggctatat ttttcatgat atatataata aaaaacacac tatcgatgag
       61 ttcaacgatg tggtaagaaa agcagtttta tcaggtaaaa ttaacgagct gaatgcatgt
      121 cataaagtgg caatttttct tgccgaaaaa gacaatgaga taacaaagaa agacaaagcc
      181 aagataatag atacattaac tgaaaactat tccattgaat ttcaacaatt aatgaatatt
      241 agtgagcgca cgttaaattc atctttatat attactcctg gtgaaagtgg ttttgtcagt
      301 tttgttaatc gtgaaggaaa aatatgtcat actgcatatg taaaatcatc agataatagc
      361 atgacatatt accatgcaaa tggtagcagc attgacaaat atattacgga tatgtgcggc
      421 cttatttgta tgaggcatat tgagtctact ggcattattt tttatatgct tgatgaaaaa
      481 gttttatctg ccattgctga atttatgaat gaaaaaggat ggcggg
//
LOCUS       DAZPAB010000521          515 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZPAB010000521 DAZPAB010000000
VERSION     DAZPAB010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.530"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            227..484
                     /locus_tag="ACHUPG_004519"
     CDS             227..484
                     /locus_tag="ACHUPG_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863792.1"
                     /translation="MKNYPTYGFYYPIANFIALQINGSKTGFAASITGFTQTISAGTI
                     SFLSVTVSDNGVGPGTTLGLGSLITSLLSIAVIAIAGNNKK"
BASE COUNT          161 a          103 c           76 g          175 t
ORIGIN      
        1 actcatgcaa atgagaaagt aatatgtcaa aacagtgcat cctttgtgac caaagagttt
       61 atctgcggca ctatacttgt ctcattaata ctcagttctc tattttgcta catatcaata
      121 tcaccatcaa ttttcatgca tacttatggc ctgagtactt ttagttactc aattattttc
      181 tccttctcag ttttattttt cataatcgga aatcaattat caaagattga aaaattatcc
      241 aacctatggt ttttattacc caatagccaa ttttatcgca ttacaaataa atggttcaaa
      301 aacaggattt gccgcatcta ttacaggttt tactcaaaca ataagcgctg gtacaatcag
      361 tttcctgtca gtaaccgtaa gcgacaacgg tgttggtccc ggaactactt taggtcttgg
      421 ttcactaata acttcattac tgtcaatagc cgtaatcgcc attgcaggaa ataataaaaa
      481 gtaagtgtac tctgactgcg tatgtatcta cttga
//
LOCUS       DAZPAB010000522          511 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZPAB010000522 DAZPAB010000000
VERSION     DAZPAB010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.531"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1..511
                     /locus_tag="ACHUPG_004520"
                     /pseudo
     CDS             1..511
                     /locus_tag="ACHUPG_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISSfl7 family transposase"
BASE COUNT          127 a          128 c          141 g          115 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt a
//
LOCUS       DAZPAB010000523          508 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZPAB010000523 DAZPAB010000000
VERSION     DAZPAB010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.532"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..>508)
                     /locus_tag="ACHUPG_004521"
     CDS             complement(<1..>508)
                     /locus_tag="ACHUPG_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR0863793.1"
                     /translation="ETFNVSWGIDENYQVGAAISIASILEKNKQNKFTFHIIADYLDK
                     EYIELLSQLATKYQTVIKLYHIDSEPLKALPQSNIWPVSIYYRLLSFDYFSARLDSLL
                     YLDADIVCKGSLNELIALEFKDEYGAVVIDVDAMQSKSAERLCNEDFNGSYFNSGVMY
                     INLREWLKQ"
BASE COUNT          176 a          100 c           68 g          164 t
ORIGIN      
        1 tttgttttaa ccattcccgt aaattaatat acattacacc agagttaaaa tagctaccgt
       61 taaaatcctc attacacaaa cgctcagcgc ttttactttg catagcatct acatcaatta
      121 ccactgcccc atattcatct ttaaactcta atgctattaa ctcgttcaat gaacccttac
      181 agacgatatc agcatcaaga tataataatg aatccaatcg cgcagaaaaa taatcaaatg
      241 aaagcaaacg ataataaata gatactggcc agatatttga ttgaggtagc gccttcaatg
      301 gctcagaatc aatatgatat aatttaatta ctgtttgata cttcgttgct aattgtgata
      361 ataattcaat atactctttg tctaagtaat cagcgattat gtgaaaggta aatttgtttt
      421 gtttattctt ttcaagaatt gaagcaattg aaatcgcagc cccaacctgg tagttctcat
      481 caatacccca tgagacatta aacgtttc
//
LOCUS       DAZPAB010000524          488 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZPAB010000524 DAZPAB010000000
VERSION     DAZPAB010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.533"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..366
                     /locus_tag="ACHUPG_004522"
     CDS             <1..366
                     /locus_tag="ACHUPG_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFR0863794.1"
                     /translation="NNGLTEAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIE
                     NMYLQDLDNSTKLYGKTGAGFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNL
                     TSSIKAKKNAITILNTLNL"
BASE COUNT          186 a          102 c           87 g          113 t
ORIGIN      
        1 aacaacggat taacagaagc atggctcgaa agtagcttaa aaatttcacc agaagaacaa
       61 attcaattcc tgcgtaaaat tattaatcac aatctcccag ttaaaaactc agccatagaa
      121 aacaccatag agaacatgta tctacaagat ctggataata gtacaaaact gtatgggaaa
      181 actggtgcag gattcacagc aaatagaacc ttacaaaacg gatggtttga agggtttatt
      241 ataagcaaat caggacataa atatgttttt gtgtccgcac ttacaggaaa cttggggtcg
      301 aatttaacat caagcataaa agccaagaaa aatgcgatca ccattctaaa cacactaaat
      361 ttataaaaaa tctaatggca aaatcgccca acccttcaat caagtcggga cggccaaaag
      421 caagcttttg gctcccctcg ctggcgctcg gcgcccctta tttcaaacgt taaacatcat
      481 gagggaag
//
LOCUS       DAZPAB010000525          488 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZPAB010000525 DAZPAB010000000
VERSION     DAZPAB010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.534"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1..488
                     /locus_tag="ACHUPG_004523"
                     /pseudo
     CDS             1..488
                     /locus_tag="ACHUPG_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          106 a          120 c          152 g          110 t
ORIGIN      
        1 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
       61 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
      121 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
      181 gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
      241 ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt
      301 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg taccgccatc
      361 gaaacgggca catacaggca gagtggccgt gaaagaaagc aatcagcgat ggtgctctga
      421 cgggttcgag ttctgctgtg ataacggaga gagactgcgt gtcacgttcg cgctggactg
      481 ctgtgatc
//
LOCUS       DAZPAB010000526          480 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZPAB010000526 DAZPAB010000000
VERSION     DAZPAB010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.535"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            32..>480
                     /locus_tag="ACHUPG_004524"
     CDS             32..>480
                     /locus_tag="ACHUPG_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR0863795.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGF"
BASE COUNT          153 a           97 c          104 g          126 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
//
LOCUS       DAZPAB010000527          475 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZPAB010000527 DAZPAB010000000
VERSION     DAZPAB010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.536"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
BASE COUNT          154 a           95 c           70 g          156 t
ORIGIN      
        1 tacgtaccgt ttaaacaaag acaatacgtc tctttatttc tcataaaacc ccaatccctt
       61 acagggctta ttgaaaaatt taaaaatcac aaatttctca atactcgaaa tgagatcaca
      121 taagaaatca aaaagccttc tcatctattt taagttttcc caacggaaaa taagagcttt
      181 cattaatact gaaccacttt actgttctat ttgttcaggt attgcattac ggtgtgctga
      241 gatccatcgg tgcatccctg tatcccgcga tgagactaaa atagtcgtga tgtaagctcg
      301 tatcagattc ttagtgaata ccacatttgt tataattaag tgaaaagata cattgctgat
      361 ttacattatt tggtttacaa aatgtcatca cttgcagagt tatcgctgac agataccttt
      421 aatctcttac tactttcgtg ttattgcaat acaaccatcc gtaaagcagg gctat
//
LOCUS       DAZPAB010000528          473 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZPAB010000528 DAZPAB010000000
VERSION     DAZPAB010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.537"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..139
                     /locus_tag="ACHUPG_004525"
     CDS             <1..139
                     /locus_tag="ACHUPG_004525"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0863796.1"
                     /translation="AGLKPDTMRNVFRCHIPRYEAIIARYLDMEPAQIWPSRYMEKTS
                     C"
     gene            172..473
                     /locus_tag="ACHUPG_004526"
                     /pseudo
     CDS             172..473
                     /locus_tag="ACHUPG_004526"
                     /inference="COORDINATES: protein motif:HMM:NF014380.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT          139 a          106 c          115 g          113 t
ORIGIN      
        1 ggcaggctta aagcctgaca ctatgcgtaa cgtatttcgc tgccatatac cacgatacga
       61 agcaatcatt gctagatatt tggatatgga accagcccag atttggcctt ctcggtatat
      121 ggagaaaaca tcatgttaac ccattgggta acagcgcaag aatgcgtagg aatgtcatgc
      181 tttcctaaac atgaggtgag tattcgccgc aatcttgaaa agcttgcagc caatcatcca
      241 gaactacgtc gcaagcgtac gggttcaaag gctttggaat ttcatatatc agttctacca
      301 gcggcagcac gtgctgagtt gttattaaaa tctgagcaaa tagaaacaag ctaggggtat
      361 tttgaaatcg cccgccccac gctggaagcg cacgattacg atcgtgaggc actgtggagc
      421 aaatgggata aagccagcga ttcccagcgc agacttgctg aaaaatggtt gcc
//
LOCUS       DAZPAB010000529          472 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZPAB010000529 DAZPAB010000000
VERSION     DAZPAB010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.538"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            3..>472
                     /locus_tag="ACHUPG_004527"
     CDS             3..>472
                     /locus_tag="ACHUPG_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HFR0863797.1"
                     /translation="MDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKL"
BASE COUNT          101 a          130 c          144 g           97 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ct
//
LOCUS       DAZPAB010000530          471 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZPAB010000530 DAZPAB010000000
VERSION     DAZPAB010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.539"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>471
                     /locus_tag="ACHUPG_004528"
     CDS             <1..>471
                     /locus_tag="ACHUPG_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR0863798.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVER"
BASE COUNT           99 a          123 c          140 g          109 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttatattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg c
//
LOCUS       DAZPAB010000531          471 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZPAB010000531 DAZPAB010000000
VERSION     DAZPAB010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.540"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..361
                     /locus_tag="ACHUPG_004529"
     CDS             <1..361
                     /locus_tag="ACHUPG_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0863799.1"
                     /translation="DHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVC
                     GEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVE
                     GLKKNARDISLLRYMPA"
BASE COUNT          128 a           99 c          161 g           83 t
ORIGIN      
        1 ggaccacaaa acgggagaaa cggcgacgga aacgctgaca cagcgtgagc tggtcgcgag
       61 gctgaaacag cacatcccgg agaagttttt taagatggtg aggtacttcg ggttccttgc
      121 caaccgggtg tgtggagaga agctgccgca ggtgtaccgt gctctgggga tggataaacc
      181 ggaaccagtg gcgaaagtgt gctatgcaca aatggtgaaa cagttcctga gtcgtgaccc
      241 gttcgaatgc gtgctgtgtg gcggccggat ggtataccgc cgggccatcg cgggactgaa
      301 tgtggagggg ctgaagaaaa acgcgcggga tatcagtctg ctgaggtata tgccggcctg
      361 acacaggcta agtgtgcccg ggaatcggtg caagtataaa aaacagcctg ttgcggtata
      421 agaatcgacc atgcagagag agaaaacacc ggagtggagg gaaaagcaga a
//
LOCUS       DAZPAB010000532          469 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZPAB010000532 DAZPAB010000000
VERSION     DAZPAB010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.541"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
BASE COUNT          119 a           95 c           92 g          163 t
ORIGIN      
        1 gtgcgttttt atctgtggtt atttagataa cattatctaa ctggacactt tcttgtatgt
       61 attatagcta aatgaacaga ttttaatatg ttgtatggtt ttcactctga aatcccctga
      121 atttaatcac gtttccttta ctgaaggcaa ttttaaccgt agtatcaatt attaaccctt
      181 gggcttgata tccctctcct cttctgggca cgggggatgt cagattactt attacagaaa
      241 ccaaaagaat tgtaagcgtc atctcagtgt cgtctatttc aatgtctgat gattggcgat
      301 gatagagtcc tcttataacg ttaatggact ctatcaatgt cagattctct tcgcccccgc
      361 aggattcgtg cctcttactc tatgactcta tggagtttaa gcttgatctt attgaaaaat
      421 cttaccagct aggggcctgc gtggcccaac tggccagaga gtacgggat
//
LOCUS       DAZPAB010000533          467 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZPAB010000533 DAZPAB010000000
VERSION     DAZPAB010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.542"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..>467)
                     /locus_tag="ACHUPG_004530"
     CDS             complement(<1..>467)
                     /locus_tag="ACHUPG_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFR0863800.1"
                     /translation="LMDKDAQGYIDLSDFDLTNCHFKGDVISKVSFLSSNLQHVTFEC
                     KEIENCNFTKATVDNVIFKCRRLHNVIFLKTSGECVDFSQNILDTVDFSQSQLGHSNF
                     RECQIRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNLTAVMFDHVRMSTGNF"
BASE COUNT          166 a           87 c           71 g          143 t
ORIGIN      
        1 taaaaattcc ctgtcgacat tcgcacatga tcaaacataa cagctgtcaa attcgattta
       61 ataaatgata tttctttggc agacagaaac tctgctctgg tgaagtgcga agcgtaaaga
      121 taacaattat cgaagtttga atttctaatc tgacattcgc gaaaattact atgaccaagt
      181 tgactctgcg agaagtcaac tgtatcaaga atattttggc taaaatcaac acattcgcca
      241 ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt taaatatgac attatcaact
      301 gttgccttag taaaattgca attctcaatt tctttacatt cgaaagttac atgttgtaga
      361 tttgatgata aaaaagacac cttagaaata acgtcacctt taaaatgaca atttgttaaa
      421 tcaaaatcag acaggtcgat atacccctgc gcatctttat ccatcag
//
LOCUS       DAZPAB010000534          465 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZPAB010000534 DAZPAB010000000
VERSION     DAZPAB010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.543"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>465
                     /locus_tag="ACHUPG_004531"
     CDS             <1..>465
                     /locus_tag="ACHUPG_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR0863801.1"
                     /translation="SGSTLISRDNKLVENFIDTLENWRDQKQIISVICKDGIKIDDSI
                     ITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDVSAATGRTSTTNDGGATSKKNTG
                     NTTTSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSVKNGESTFTASA"
BASE COUNT          147 a           96 c          102 g          120 t
ORIGIN      
        1 aatccggtag tacccttatc tcacgagata ataagctggt ggagaacttc atcgacactc
       61 ttgaaaactg gcgtgaccag aagcagatta tttcagttat ctgtaaagat ggtattaaga
      121 ttgacgactc cattattaca agctttaaag cctctaaaga tgttggaatt tcaaacggac
      181 tgagaatcca gctaactttt caggaaatta acttcaaagc tattgtaggg caaactgatg
      241 tttcagcagc cactggacgg acttctacca cgaatgatgg cggtgctacc agtaaaaaga
      301 acactggcaa cactacgaca agtcttggaa gccctatgct gacctgtaaa gagcttttta
      361 gctattcggc aagtgagtta tcagatgaag cgttaaaagc ccgtgtaacg tgctctaaga
      421 gcgttagcgt taaaaatggt gagagcacct tcacagcctc agcta
//
LOCUS       DAZPAB010000535          462 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZPAB010000535 DAZPAB010000000
VERSION     DAZPAB010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.544"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..229
                     /locus_tag="ACHUPG_004532"
     CDS             <1..229
                     /locus_tag="ACHUPG_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393504.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFR0863802.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
BASE COUNT          154 a           97 c           81 g          130 t
ORIGIN      
        1 ggaaagcaat tctgacagcg ccccctatac gttattaaaa aactatgctg acagatttaa
       61 aaaatattca gcagaagagc agtacgaaat tgattttcaa catcgactgg taaacagaaa
      121 ttgttattta gataaatatg gcgatgcacg aatcaatgct tcttatacac aattagaaat
      181 aaaacactca caacccaaaa atagagcatc cggcaaaaga gtgagttaac atcattgtta
      241 atcaattcga ccagtcagta aatatgctga ctggtcgaac ataatttgct aaaaagtcat
      301 cactgactga tgtcagtccc accatctgcc agttcaatct acgggtattt ccagcatatc
      361 gcggcacgcg attgctggtc tgttgatgta gtggattgta gttgctgact tttcttaccc
      421 tcagatttct gccccacttg tcaattcaat ctgccaataa at
//
LOCUS       DAZPAB010000536          459 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZPAB010000536 DAZPAB010000000
VERSION     DAZPAB010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.545"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>459
                     /locus_tag="ACHUPG_004533"
     CDS             <1..>459
                     /locus_tag="ACHUPG_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR0863803.1"
                     /translation="SAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWVSE
                     MADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGSSL
                     PAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGC"
BASE COUNT          116 a          109 c          141 g           93 t
ORIGIN      
        1 ccagtgcagg gttacttgca cggatcctgg tcagcaaata tatggaacat atccctttat
       61 atcgccagtc agaaatatac gcgcgacagg gcgtggagct gagccgtaat accatggtgc
      121 gctgggtatc agaaatggca gacaaactcc gtcctctgta tatagcgctg aatgactatg
      181 ttctggaggc aggaaaggtg cacgcagatg acactccggt gaaagtactg gccccgggga
      241 acggaaagac gaaaacgggt cgtctgtggg tatacgtcag ggatgatcgt aatgcgggtt
      301 catccctgcc ggcagccgtc tggttcgcgt attcggcaga tcgcaaagga gaacatccgc
      361 agctccacct ggcaaagtat cagggcgtac tgcaggctga tgcctatgca ggttataacg
      421 tactgtacga aacgggccgg gtgaaggaag ccgggtgcc
//
LOCUS       DAZPAB010000537          459 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZPAB010000537 DAZPAB010000000
VERSION     DAZPAB010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.546"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..>459)
                     /locus_tag="ACHUPG_004534"
     CDS             complement(<1..>459)
                     /locus_tag="ACHUPG_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HFR0863804.1"
                     /translation="LSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLLM
                     FNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVRE
                     KLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEHDAGFA"
BASE COUNT          106 a          117 c           90 g          146 t
ORIGIN      
        1 gggcaaatcc agcatcatgt tcgaacacca ctccaacatc tgtttgaaca cctgttaaat
       61 ttctctcttg ttttgtttga acatctgctt cctttctgct tcttctggcc tgaacagatg
      121 cttttcctgc ggctgatttt ttggttaatt tttctctgac agatgccaga tcttcctcaa
      181 tccgaagatg catccattcg tcgccgttat cacaaaaaaa ctcccgcaag gatggttcaa
      241 catcaaccca tcgctcgtta gtcagacggg caatttttgc caacctgttt ttgggtattg
      301 gctttcctgt ttgccagtaa ttgaacatca gcaacaaata cgcgccgtgc tcctctgctg
      361 acaaatgcat ggtgtcagcc aggtaatcag ctatgtacag ttgcatgtat ggtaatgcgg
      421 ccataattgc cccgtatgat gctacccggt tgcttagaa
//
LOCUS       DAZPAB010000538          446 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZPAB010000538 DAZPAB010000000
VERSION     DAZPAB010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.547"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..379)
                     /locus_tag="ACHUPG_004535"
     CDS             complement(<1..379)
                     /locus_tag="ACHUPG_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863805.1"
                     /translation="MNVVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFG
                     GMVRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEF
                     DIWNLNETWAFQEKLIFCEDESSL"
BASE COUNT          134 a          106 c           96 g          110 t
ORIGIN      
        1 acagacttga ttcatcctca caaaatataa gtttttcctg gaatgcccat gtctcattga
       61 ggttccagat atcaaattcg aagtcgtgat aacgaaaacg gatcccacca aacttattaa
      121 aacgcagttg tttgacgggc agttcagcca atgtctggaa gagttcctca cgggagcgac
      181 cgatgacgat atcaaggtct gaagagaatc catcgacgcc atacaagcct aagtcacgca
      241 ccatcccgcc aaaaatcctg gccggacagg aacaggccag agtatcaagt aatactctgg
      301 ctttcttatg ctcatcacta agcgtaaaaa aatcggcgat acgctgttca agcagttggg
      361 tgtttgagac cacattcata cagtgatttt agctcatcgt gaaggcgcat taaataacta
      421 tccgtcatgc ctgagattgc gtcggt
//
LOCUS       DAZPAB010000539          443 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZPAB010000539 DAZPAB010000000
VERSION     DAZPAB010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.548"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>443
                     /locus_tag="ACHUPG_004536"
     CDS             <1..>443
                     /locus_tag="ACHUPG_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0863806.1"
                     /translation="ESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGT
                     KAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWE
                     SIEMVRRYAHLAPNHLTEHARQIDLILNPSVPNLSQSRNKEGTND"
BASE COUNT          136 a           97 c          115 g           95 t
ORIGIN      
        1 agagagtaaa tcaaaccgcg caattggcgt tgcgctgaat gatactgcat gtcgcgtatt
       61 gaaaaaacaa atcggaaatc atcaccgttg ggtatttgtg tacaaggaaa gctgtaccaa
      121 accagacgga acgaaagcgc caacagtaag gaagatgcgg tatgacgcaa acacagcctg
      181 gaaagcggcg ctgagacggg ctggtattga tgatttcaga tttcacgact tgagacacac
      241 ctgggcaagt tggctggttc aagccggagt cccgttatca gtgttacagg aaatgggagg
      301 ctgggagtct atcgaaatgg ttcgtcgata tgctcacctc gcacctaatc accttaccga
      361 acacgcacgg caaatagact tgattctgaa cccatcggtc ccaaatttgt cccagtcaag
      421 aaataaggaa ggtactaatg atg
//
LOCUS       DAZPAB010000540          443 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZPAB010000540 DAZPAB010000000
VERSION     DAZPAB010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.549"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            213..>443
                     /locus_tag="ACHUPG_004537"
     CDS             213..>443
                     /locus_tag="ACHUPG_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFR0863807.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDSRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          141 a          105 c           61 g          136 t
ORIGIN      
        1 tatcgaacct cgcttaccaa cgcgttgtca gtcatctccc ccctctctgg tgcatcgtta
       61 tatccgtgat gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt
      121 cagaaaacaa acttattttt attaaaatca attctttttc tttgaacatt caactgcgat
      181 atttgtaaaa acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat
      241 tccccgcata tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc
      301 aatacgcacg cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatagcagac
      361 gtactgttga gatccacaaa ctagatctct ctgactggca agcattcaat aagttagcaa
      421 cgaagtgtaa tgcttacgat gga
//
LOCUS       DAZPAB010000541          437 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZPAB010000541 DAZPAB010000000
VERSION     DAZPAB010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.550"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..269
                     /locus_tag="ACHUPG_004538"
     CDS             <1..269
                     /locus_tag="ACHUPG_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0863808.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..>437
                     /gene="tnpB"
                     /locus_tag="ACHUPG_004539"
     CDS             266..>437
                     /gene="tnpB"
                     /locus_tag="ACHUPG_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0863809.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKIL"
BASE COUNT          109 a          114 c          123 g           91 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtggcaa
      421 aatggtgaaa atactgt
//
LOCUS       DAZPAB010000542          434 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZPAB010000542 DAZPAB010000000
VERSION     DAZPAB010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.551"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            35..434
                     /locus_tag="ACHUPG_004540"
                     /pseudo
     CDS             35..434
                     /locus_tag="ACHUPG_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAZPAB010000543          431 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZPAB010000543 DAZPAB010000000
VERSION     DAZPAB010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.552"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1..149
                     /locus_tag="ACHUPG_004541"
                     /pseudo
     CDS             1..149
                     /locus_tag="ACHUPG_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(169..>431)
                     /locus_tag="ACHUPG_004542"
     CDS             complement(169..>431)
                     /locus_tag="ACHUPG_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HFR0863810.1"
                     /translation="STGRILVLSLLATLSTIVMWLLGYHAENKGLHLKYQANSIKSRR
                     VISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRNMVLVY"
BASE COUNT          110 a          104 c          101 g          116 t
ORIGIN      
        1 cgcgcgaagt gttgtatgac aatatgaaaa ctgtggttct gcaacgtgac gcatatcaga
       61 ccggtcagca ccggttccat ccttcgctgt ggcagttcgg caaggagatg ggcttctctc
      121 cccgactgtg tcgccccttc aggctgaggg atccccacaa aattactgct aataaaccag
      181 caccatattt cggtaggttc tggacagatg gtttagaact gtgctcagta ctgttcgtct
      241 taaaataagt ggcgagtgtc gaagaacatt cttcgctaac gtcagatatg agataacccg
      301 ccgggattta atgctgttag cctgatattt caggtgtaac cctttatttt cagcgtgata
      361 accgagtaac cacattacga tagtgctcaa cgttgccagc agactcagaa ccagaattct
      421 tcctgttgaa c
//
LOCUS       DAZPAB010000544          418 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZPAB010000544 DAZPAB010000000
VERSION     DAZPAB010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.553"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..>418)
                     /locus_tag="ACHUPG_004543"
     CDS             complement(<1..>418)
                     /locus_tag="ACHUPG_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR0863811.1"
                     /translation="NRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDP
                     DWVSKITHLDQDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVG
                     PTKPWHEWADYPVSRSFLIAKAASPWSKEDLLKPVN"
BASE COUNT          129 a           82 c           68 g          139 t
ORIGIN      
        1 ctatttacag gtttaagtaa atcttcttta ctccacggag aagctgcttt ggcgatcaaa
       61 aaactacgtg aaaccggata atcagcccac tcatgccagg gttttgtagg tcccacatag
      121 tgtataaaca cggtgtcatc attgactgga ttatcaactt tgtcttttaa ttcatagtta
      181 atactatatc gggtattata tttctccgaa ataaacttca ctttaccatt caataataca
      241 ttcagtacat cttgatcaag gtgggtgatt ttacttaccc aatctgggtc acgcaacatt
      301 tcaatagctt ttgacgaaat gttatttaga ttccactcat caatatttat cagtaaaaaa
      361 ccagcattga aatatccaga agctaactgt ggtgtagtta ataccgaggc tcgatttt
//
LOCUS       DAZPAB010000545          412 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZPAB010000545 DAZPAB010000000
VERSION     DAZPAB010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.554"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>412
                     /locus_tag="ACHUPG_004544"
     CDS             <1..>412
                     /locus_tag="ACHUPG_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFR0863812.1"
                     /translation="RAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQV
                     NGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGK
                     DHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFL"
BASE COUNT          116 a           75 c           97 g          124 t
ORIGIN      
        1 cgcgccgtga ttctgaaaat ggaaatgatg ccatttttag actcggtggg actggtactt
       61 gatgataata aatattatct cttttcgcgg agggcgaatg ataaaatcgt tgtttatcat
      121 caggaacaag taaatggacc gcttgtcgac gagtcagggc gggttatttt tgccgatttt
      181 aacccatcga aacgaccgtg gtcggtggct tcagatgact ctaacaacag ctggaatccg
      241 gcatacaatt gctttgatcg tccgggtaaa aaatgtatct cttttacgct acgtatcaac
      301 ggcaaagatc acgatttgtt agcggtagat aaaattcatg tcgatttaaa ctggcggtat
      361 ctgaacgagt atcttgatca aatcagcgct aatgatgaag ttctattttt ga
//
LOCUS       DAZPAB010000546          412 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZPAB010000546 DAZPAB010000000
VERSION     DAZPAB010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.555"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1..412
                     /locus_tag="ACHUPG_004545"
                     /pseudo
     CDS             1..412
                     /locus_tag="ACHUPG_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          100 a          114 c          121 g           77 t
ORIGIN      
        1 gaacgtgaac tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg
       61 gatgaagagc tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga
      121 agtatgtctt tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc
      181 tccgggtggc ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc
      241 gtcagcagtt ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac
      301 agcgttacgg tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg
      361 taaaaaccgt ggcggcaagc ctgcgccgtc agggactgag ggcaaaggcc tc
//
LOCUS       DAZPAB010000547          409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZPAB010000547 DAZPAB010000000
VERSION     DAZPAB010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.556"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            155..340
                     /locus_tag="ACHUPG_004546"
                     /pseudo
     CDS             155..340
                     /locus_tag="ACHUPG_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          118 a           97 c          108 g           86 t
ORIGIN      
        1 tacggtggtc ggtttacgct taccgcagga caggtggtaa ctgaaaaata gtttgctgag
       61 gcgactggaa acagaagggc aagatcgatt ccgacagcag tgatggtcag ctacctcatc
      121 gataactttg caacagtgcc cttcttgaag catcatgctt catgaagaaa aactggcacg
      181 tcatcaacgt aaacaggcga tgtatacccg aatggcagcc ttcccggcgg tgaaaacgtt
      241 cgaagagtat gacttcacgt tcgccaccgg agcaccgcag aagcagctcc agtcgttacg
      301 ctcactcagc ttcatagagc gcaatgaaaa catagtttta caggggacat ctgacatcac
      361 aaatccccgg gtggggattt gtgtataaga gacagcccat ggaggaagg
//
LOCUS       DAZPAB010000548          390 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZPAB010000548 DAZPAB010000000
VERSION     DAZPAB010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.557"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(42..>390)
                     /locus_tag="ACHUPG_004547"
     CDS             complement(42..>390)
                     /locus_tag="ACHUPG_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0863813.1"
                     /translation="QIKPFLEKAMTMYNDVLFPGEDINKPISIAAANRFVNRIRGGMD
                     LGYWRTHDFRRTLVTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELH
                     ADKLFWHIRSISD"
BASE COUNT           92 a           91 c           87 g          120 t
ORIGIN      
        1 ccttcgtgaa gctgttgaag ggtggattct taacggtggc gttaatcaga aatgctcctg
       61 atgtgccaga acaatttatc agcgtgaagc tcatacgctt tgcgctgagc ctctatccag
      121 tcgtgtttat tgtatacgga cattatcccg ccaagttcat gaccgagcat tcgctcagta
      181 acatgaggct cgacattcat ctcggacagc cgtgtaacaa gcgttcttct gaaatcatgt
      241 gttcgccagt aacctagatc catccctccc cttattctat ttacgaatcg gttggctgca
      301 gcgatgctga tcggtttgtt tatgtcttct ccagggaaaa gaacatcatt gtacattgtc
      361 atggcctttt cgagaaaagg cttaatttgt
//
LOCUS       DAZPAB010000549          384 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZPAB010000549 DAZPAB010000000
VERSION     DAZPAB010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.558"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(1..252)
                     /locus_tag="ACHUPG_004548"
                     /pseudo
     CDS             complement(1..252)
                     /locus_tag="ACHUPG_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           88 a          123 c           86 g           87 t
ORIGIN      
        1 ctcactgtct ccgttatggc gtacaccaca tcttccagta atcgccgctt caccggactc
       61 aggaccccgt atcaccatcc ggaacccctt atccgccaca tgccaggaga acctttccat
      121 aaactcgtcg ccactcatca caaggttttc ttctcgtttt gtccggtggc tgttgtaacg
      181 caggccaatt tcatcctgac cggcataatg ttccaggcgg ctcatctgca ccggctgctt
      241 ctccagccag ttaagccatc ggtttccctg ctgaaaaata tcggcaaaac ggggaagcat
      301 cagaaaggcg gaataacgcc atccggccag tgaaccgtac cgcattcagg gcaatacatg
      361 ccgtcggcgt tgataccggc agga
//
LOCUS       DAZPAB010000550          383 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZPAB010000550 DAZPAB010000000
VERSION     DAZPAB010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.559"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
BASE COUNT          140 a           57 c           73 g          113 t
ORIGIN      
        1 taatgaaaaa attgcaatat gaatatttac aaaatacctt gacccgtttc cggttgcaag
       61 tgttggcagt tgaggaacag aatctgccag ttgaggtttt gccatagaaa atgcgacaat
      121 aagtattgga agggtggcaa acactttcat ttgccaattt cctttaatat aaatgaaaac
      181 acataatgta attactaaag ttgatagtgt gtacgataaa aggtgattgt cccatccagc
      241 attagcgata ttccaaggga caaatgaaaa aagaaataca ttagatgata ttatagatga
      301 cataacatca aaactgaatc ctaatggagc atgtgatctt gttccattaa atgtaagaat
      361 tattgatgtt gcctgtatca gcc
//
LOCUS       DAZPAB010000551          375 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZPAB010000551 DAZPAB010000000
VERSION     DAZPAB010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.560"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>375
                     /locus_tag="ACHUPG_004549"
     CDS             <1..>375
                     /locus_tag="ACHUPG_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0863814.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRLMRQNALLLERKLAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDRKALHWAVTT"
BASE COUNT           86 a           96 c          110 g           83 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggctca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aactcgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgta aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DAZPAB010000552          374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZPAB010000552 DAZPAB010000000
VERSION     DAZPAB010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.561"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>374
                     /locus_tag="ACHUPG_004550"
     CDS             <1..>374
                     /locus_tag="ACHUPG_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0863815.1"
                     /translation="DVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALE
                     MAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRS
                     LKNEWMPVVGYVSFSEAAHAIT"
BASE COUNT           89 a           91 c          121 g           73 t
ORIGIN      
        1 gatgtgacct atatctggac gggtaagcgc tgggcgtacc tcgccgttgt tctcgacctg
       61 ttcgcaagaa aaccagtggg ctgggccatg tcgttctcgc cggacagcag gctcaccatg
      121 aaagcgctgg aaatggcatg ggaaacccgt ggtaagcccg gcggggtgat gttccacagc
      181 gatcagggca gtcattatac gagcaggcag ttccggcagt tattgtggcg ataccagatc
      241 agacagagta tgagccggcg cggaaactgc tgggataaca gcccaatgga acgcttcttc
      301 aggagtctga agaacgaatg gatgccggtg gtgggttacg taagcttcag cgaggcagct
      361 cacgccataa cgga
//
LOCUS       DAZPAB010000553          372 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZPAB010000553 DAZPAB010000000
VERSION     DAZPAB010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.562"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..311)
                     /locus_tag="ACHUPG_004551"
     CDS             complement(<1..311)
                     /locus_tag="ACHUPG_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0863816.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAZPAB010000554          371 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZPAB010000554 DAZPAB010000000
VERSION     DAZPAB010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.563"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
BASE COUNT          108 a           81 c           69 g          113 t
ORIGIN      
        1 gttaattttg gtgtttaaac cttttttact tgttagtatg atacaaaggc tttcaaaaaa
       61 gctgggaaat tcaacaactc ttggaaatta tggactttgt cccgcggctt taatctgacg
      121 gccgcgttcc ttttttggct caattattga ccccttctct acgcaacttc agttcccgcc
      181 agcaactttg cggcagtttc ataggatcaa tgtctaaaag aataatggtg accgataaga
      241 aaacgactga ataactgtag attttcgctc gaaaccttcc tgtcagctcc atagcgaatc
      301 aagcgctgaa tgtcacagta tcgaacagaa aacagtgacg atctaaccct tcaagaatat
      361 tctacgattg t
//
LOCUS       DAZPAB010000555          369 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZPAB010000555 DAZPAB010000000
VERSION     DAZPAB010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.564"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
BASE COUNT          116 a           82 c           71 g          100 t
ORIGIN      
        1 agagaatcaa tattttacca gagaggacta tacgtataat tacgaccttt taaatgcaat
       61 atagacttaa taacttctta caaagtctat tattaatacc gtagatattc atcttaccct
      121 gtgcaccaaa agcaaaaact tatcgacgcg ggtttgaaac tgatggcaac cgcaaaaatg
      181 cctgatgcgc tacgcttatc aggcctacgc catctctgca atatattgaa tttgcgtgct
      241 tttgtaggca ggataaggcg ttcacgcagc atccggcatg aataaagcgc actcaacaat
      301 ctgaaacccg ccggaacggt ttattacgta catcaggtga aactgaccga taagccgctt
      361 tcttttggg
//
LOCUS       DAZPAB010000556          367 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZPAB010000556 DAZPAB010000000
VERSION     DAZPAB010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.565"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1..367
                     /locus_tag="ACHUPG_004552"
                     /pseudo
     CDS             1..367
                     /locus_tag="ACHUPG_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
BASE COUNT           78 a           93 c          115 g           81 t
ORIGIN      
        1 cggtacgcac cagaaaatca ttgatatggc catgaatggc gttggatgcc gggcaaccgc
       61 ccgcattatg ggcgttggcc tcaacacgat tttccgccat ttaaaaaact caggccgcag
      121 tcggtaacct cgcgcataca gccgggcagt gacgtcatcg tctgcgcgga aatggacgaa
      181 cagtggggat acgtcggggc taaatcgcgc cagcgctggc tgttttacgc gtatgacagg
      241 ctccggaaga cggttgttgc gcacgtattc ggtgaacgca ctatggcgac gctggggcgt
      301 cttatgagcc tgctgtcacc ctttgacgtg gtgatatgga tgacggatgg ctggccgctg
      361 tatgaat
//
LOCUS       DAZPAB010000557          365 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZPAB010000557 DAZPAB010000000
VERSION     DAZPAB010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.566"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>365
                     /locus_tag="ACHUPG_004553"
     CDS             <1..>365
                     /locus_tag="ACHUPG_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFR0863817.1"
                     /translation="HQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQ
                     LWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSEN"
BASE COUNT           76 a          124 c          102 g           63 t
ORIGIN      
        1 tcaccagatt gtgaatcgcc tggacatcat gctgccgggc aatcgcccct tcgttgcccc
       61 cagtgccgtt attcaggccc gccagcgcct gggaagtgag gctgtccgcc gcgtgttcac
      121 gaaaacagcg cagctctggc ataacgccac gccgcatccg cactggtgcg gcctgaccct
      181 gctggccatc gatggtgtgt tctggcgcac accggataca ccagagaacg atgcagcctt
      241 cccccgccag acacatgccg ggaacccggc gctctacccg caggtcaaaa tggtctgcca
      301 gatggaactg accagccatc tgctgacggc tgcagccttc ggcacgatga agaacagcga
      361 aaatg
//
LOCUS       DAZPAB010000558          357 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZPAB010000558 DAZPAB010000000
VERSION     DAZPAB010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.567"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            241..348
                     /gene="ldrD"
                     /locus_tag="ACHUPG_004554"
     CDS             241..348
                     /gene="ldrD"
                     /locus_tag="ACHUPG_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HFR0863818.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           96 a           82 c          104 g           75 t
ORIGIN      
        1 cagagtgaag atgagacgga ttccggaaca gaaaaacagt cgtagaatca gcgtcaagcc
       61 ggaaggattc cggtgaggcg caatgttgcg ggggcttaat ccctggtggc attggttgct
      121 ggagagagaa aacccccgca cgttgcaggt atgcacctga caacaccacg ggggccattc
      181 ttgcatccaa acaatacaag aatagccgcg aaacgttgtc attacaatac aggcggctat
      241 atgacgttcg cagaactggg catggccttc tggcatgatt tagcggctcc ggtcattgct
      301 ggtattcttg ccagtatgat cgtgaactgg ctgaacaagc ggaagtaacg tgccatg
//
LOCUS       DAZPAB010000559          357 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZPAB010000559 DAZPAB010000000
VERSION     DAZPAB010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.568"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            39..>357
                     /locus_tag="ACHUPG_004555"
     CDS             39..>357
                     /locus_tag="ACHUPG_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR0863819.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          112 a           86 c           91 g           68 t
ORIGIN      
        1 gcctggcatc cggatataac ccgtgataaa acaagggaat gaacaatgaa ctccccgatg
       61 atattgagct gcttaaagcc atgttgcgta agcaacagag tcggcttcga cagtatgcct
      121 gtcaggtcgc gggctatgag caggaaattg aacggctgaa agcgcaactc gacaggttgc
      181 gtcgtatgtt gttcggccag agttcagaga aaaagcgtca taagcttgaa aatcagatcc
      241 gacaggcaga aaaacgactg tcggaactgg aaaaccggct gaacacagcc agaaatcttc
      301 tggaagatgc atcgtcagtc acagattcac ctgacaccag tcccccgtca gaaaacc
//
LOCUS       DAZPAB010000560          353 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZPAB010000560 DAZPAB010000000
VERSION     DAZPAB010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.569"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..264
                     /locus_tag="ACHUPG_004556"
     CDS             <1..264
                     /locus_tag="ACHUPG_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0863820.1"
                     /translation="FYRNDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISD
                     EDKEKLKACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            288..>353
                     /locus_tag="ACHUPG_004557"
     CDS             288..>353
                     /locus_tag="ACHUPG_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR0863821.1"
                     /translation="MSVVISGALTDGAGIPMSGYHI"
BASE COUNT           96 a           80 c           90 g           87 t
ORIGIN      
        1 ttttaccgca atgatgaatt tgtctttgac tacagactca aagcggaaga tgagcgtgat
       61 gcccttctgg ctcaggtcag tgcccggaca ggggaatggg aagaagacct gctgctgggg
      121 ttaatcagcg acgaagataa agaaaagctg aaagcctgtc gtatttacgc gaaatcgctg
      181 caggcgatgg atttcagcac catcactgat aaatcctcat acaacgccat tgaatggccc
      241 gcctctccgg aaggttcttc ctgatttaat ttatcgcgag aaaaacaatg tctgtagtga
      301 tatcaggtgc gctgactgat ggcgcaggta tccccatgtc cggataccat att
//
LOCUS       DAZPAB010000561          347 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZPAB010000561 DAZPAB010000000
VERSION     DAZPAB010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.570"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>347
                     /locus_tag="ACHUPG_004558"
     CDS             <1..>347
                     /locus_tag="ACHUPG_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863822.1"
                     /translation="LAFDSFKERLESTMAALTFFISTGQCDCDEIAESNFNRMIAYMS
                     NINYDASKLGAPALSFDTYLQDNVKYRVIINNLYGSEFRIRDINENFVGMDFTCEFRP
                     EKMTNLINIKNRLV"
BASE COUNT          109 a           55 c           74 g          109 t
ORIGIN      
        1 ctggctttcg actcttttaa agaaaggctt gagtctacca tggcggcact cactttcttt
       61 atatcaacgg gtcaatgtga ctgcgatgaa atagcagagt cgaacttcaa tcgtatgatt
      121 gcctatatgt ctaacattaa ctatgatgcc tcaaagctag gagcgccagc acttagtttt
      181 gatacgtatc tgcaggacaa tgttaaatat agagtgataa ttaataatct ttatggcagt
      241 gagtttcgga taagagatat taatgagaat tttgttggga tggattttac ctgtgaattc
      301 cgacccgaaa aaatgacgaa tttgatcaat ataaaaaata ggttggt
//
LOCUS       DAZPAB010000562          342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZPAB010000562 DAZPAB010000000
VERSION     DAZPAB010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.571"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(42..134)
                     /locus_tag="ACHUPG_004559"
                     /pseudo
     CDS             complement(42..134)
                     /locus_tag="ACHUPG_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          108 a           59 c           84 g           91 t
ORIGIN      
        1 ttaagtgata ctggttgtct ggagattcag ggggccagtc tagcagttga cggggtatct
       61 gctgcgctgg ccatgagagg aagagcggcg gcgacagcaa caacaagacg tttgggtgaa
      121 aacgaaaatt ccatgcaaaa tgctccggtt tcatgtcatc aaaatgatga cgtaattaag
      181 cattgataat tgagatccct ctccctgaca ggatgattac ataaataata gtgacaaaaa
      241 taaattattt atttatccag aaaatgaatt ggaaaatcag gagagcgttt tcaatcctac
      301 ctctggcgca gttgatatgt aaggcaggtt tattatatcg cg
//
LOCUS       DAZPAB010000563          341 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZPAB010000563 DAZPAB010000000
VERSION     DAZPAB010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.572"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..171
                     /gene="xisR"
                     /locus_tag="ACHUPG_004560"
     CDS             <1..171
                     /gene="xisR"
                     /locus_tag="ACHUPG_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFR0863823.1"
                     /translation="LMLRTGLGARQIEAYRQNCWVEGFHFKRVSPLGKPDSKRGIIWY
                     NYPKINQFIKDS"
     gene            173..>341
                     /locus_tag="ACHUPG_004561"
     CDS             173..>341
                     /locus_tag="ACHUPG_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFR0863824.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHQISS"
BASE COUNT          108 a           64 c           83 g           86 t
ORIGIN      
        1 ctgatgcttc gcactggtct gggtgccaga caaattgaag cataccgcca gaactgttgg
       61 gtggaaggct tccacttcaa acgagtatct cctttaggga agccagacag taagcgaggg
      121 attatctggt acaactatcc gaagataaat cagtttatca aagactcatg atatgtctaa
      181 attaccaaca ggtgtcgaga ttcgaggtaa atacattcgc atctggttca tgtttcgagg
      241 aaaacgatgt cgggaaacat tgaaaggctg ggagattact aacagtaaca ttaaaaaggc
      301 cgggaattta agagcgttga tagttcatca aatcagttcc g
//
LOCUS       DAZPAB010000564          338 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZPAB010000564 DAZPAB010000000
VERSION     DAZPAB010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.573"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
BASE COUNT          103 a           63 c           68 g          104 t
ORIGIN      
        1 agagaaataa tatttattaa aatggcaggt cttctttttc tgattaccga agacaaagtg
       61 gcaattacgt atagtttccc gataaagacg cgacagctac gtcccgcata aggcattttt
      121 ctctttatct ttgtacggta cttcatggaa cagaattttt gaccttgtga atcgtgatat
      181 ctgttgggga ggggcaattt gctcactgaa gcgtgagact cgattaagcg cacgaaacac
      241 agaaatcaaa aaacccggcc acttttttac aaagtggccg ggtaaaaata atttttattt
      301 tttaactgtc ctgagtctca tagagatgtc tcaaaact
//
LOCUS       DAZPAB010000565          338 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZPAB010000565 DAZPAB010000000
VERSION     DAZPAB010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.574"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..>338)
                     /locus_tag="ACHUPG_004562"
     CDS             complement(<1..>338)
                     /locus_tag="ACHUPG_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HFR0863825.1"
                     /translation="YKWLLIPLLILMFTLTYKLKTRSIYLISSIIFIYILPVIFIVVV
                     GSGAPPRLFVLMPIVAVILFSCLSNFRSIKYLNCMFFLFIIFNGVSTSKNLFLNDTLA
                     RQKDISLAKE"
BASE COUNT          138 a           44 c           56 g          100 t
ORIGIN      
        1 tttctttagc taaagagata tctttctgtc ttgcgagagt atcattcaaa aatagatttt
       61 tggatgttga aacgccatta aatataataa ataaaaaaaa catgcagttt aggtatttta
      121 tagagcgaaa attgcttaag caagaaaaca aaattactgc tactataggc attaaaacaa
      181 acaggcgagg tggcgcccct gagccaacaa caacgataaa tataaccgga agtatataaa
      241 tgaaaatgat tgatgaaatc aaataaattg atcttgtttt taatttatat gttagggtaa
      301 acatcagaat taataaagga atcagtaacc acttgtat
//
LOCUS       DAZPAB010000566          335 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZPAB010000566 DAZPAB010000000
VERSION     DAZPAB010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.575"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>335
                     /locus_tag="ACHUPG_004563"
     CDS             <1..>335
                     /locus_tag="ACHUPG_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0863826.1"
                     /translation="NGKVIAPACTLVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVP
                     IALEGCDVTVTKNATFTFSGTADGVQPTAFANQATTDAATNVALQMYLPDGSTSVTPG
                     TETSNIQLA"
BASE COUNT           92 a           79 c           78 g           86 t
ORIGIN      
        1 taatggtaaa gttattgctc cagcttgtac cctggtagct gcgacgaaag attcagtagt
       61 cacattgcca aatgtcagcg ccaccaagtt gcaaactaat ggagcagtct ctggcgttaa
      121 aactgatgta ccaattgcct tagaaggctg tgatgttact gtaactaaaa atgcgacgtt
      181 tacctttagt ggtacggctg acggcgtgca acctacagca tttgccaacc aggctaccac
      241 cgatgcagcg acgaatgttg ctctacaaat gtatctgcca gatggttcta catcggtaac
      301 tcccggtacc gaaacgtcga atattcagtt agcgg
//
LOCUS       DAZPAB010000567          334 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZPAB010000567 DAZPAB010000000
VERSION     DAZPAB010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.576"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(43..330)
                     /locus_tag="ACHUPG_004564"
     CDS             complement(43..330)
                     /locus_tag="ACHUPG_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863827.1"
                     /translation="MDEEYRKDLQLWFGLTHASFCVMPRVFMEAMPPEWQEKMAQLLF
                     EYSDTIKTDVCGVHSCFVTAKDGNNRFMRMPEDILNYRHPRREFIESFLKK"
BASE COUNT           91 a           86 c           67 g           90 t
ORIGIN      
        1 taagccatta tcaaacccac ccgtagatag gctttgtaat ggctacttct tcagaaatga
       61 ttcgatgaat tcacgtcgag gatgacgata gttcagaata tcttctggca tcctcataaa
      121 gcggttgttg ccgtctttgg cagtaacaaa acagctgtga actccgcaga catccgtttt
      181 gatcgtgtcg ctatactcaa aaagcaactg agccatcttc tcttgccatt ctggaggcat
      241 agcctccatg aatactcgcg gcatcacgca gaacgacgca tgcgtaagac caaaccacag
      301 ttgcaggtct ttacgatatt cttcatccat cgtc
//
LOCUS       DAZPAB010000568          332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZPAB010000568 DAZPAB010000000
VERSION     DAZPAB010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.577"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            51..>332
                     /locus_tag="ACHUPG_004565"
     CDS             51..>332
                     /locus_tag="ACHUPG_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0863828.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPD"
BASE COUNT           83 a           70 c           98 g           81 t
ORIGIN      
        1 cgtccgaatg ttagccattc gagggttgtt aagtagtttc aggaggattt atggcgaaac
       61 cttttactcc cgaacagcgg gaagaactga agacgcgaat tgtggaactc gtgcatcagg
      121 acggtcgggt cacgattcgg cagttgtccg atgaaacagg tatcagtcgt gcgtctgtcg
      181 gtcgcttatg catagaactg gtcgcaagtg gtgatgtata taattctggc tacggcttat
      241 tcccgtctga acaggctcgc aaggactggc aaagcgcccg caaaaaactc tcgagagtaa
      301 aggtgaggaa accggttgtt gttgatccgg ac
//
LOCUS       DAZPAB010000569          332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZPAB010000569 DAZPAB010000000
VERSION     DAZPAB010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.578"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>332
                     /locus_tag="ACHUPG_004566"
     CDS             <1..>332
                     /locus_tag="ACHUPG_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0863829.1"
                     /translation="QYLLSLVPYCPWQHIVFTLPCQYWSLVFHNRWLLAEMSRIAADV
                     ILEICHQADVEPGIFTVIHTWGRDQQWHPHIHLSTTAGGVTSGHTWKNLHFYARKVMS
                     MWRYRITR"
BASE COUNT           71 a           88 c           95 g           78 t
ORIGIN      
        1 tacagtatct gctgagcctg gtcccctact gcccgtggca gcatattgtg ttcacacttc
       61 cctgccagta ctggtccctg gtgttccaca accggtggtt actggcagag atgagccgca
      121 ttgcagcgga tgtgatactg gaaatctgcc atcaggcaga tgtggagccg gggatattca
      181 cggtgatcca cacatggggg cgtgaccagc agtggcatcc gcatatccat ttatcgacaa
      241 ctgccggtgg tgtgacgtcg ggccacacct ggaaaaatct tcatttttac gcccgtaagg
      301 tgatgagcat gtggcgctac cggataacac gg
//
LOCUS       DAZPAB010000570          325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZPAB010000570 DAZPAB010000000
VERSION     DAZPAB010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.579"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            103..>325
                     /locus_tag="ACHUPG_004567"
     CDS             103..>325
                     /locus_tag="ACHUPG_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR0863830.1"
                     /translation="MIYSNQLRYTGKDGVYFHLQGQPDNFLSLSATENMEFDSPMMVT
                     TQNMQSGQTVTDNVMRSPRTISISGVVVVG"
BASE COUNT          110 a           56 c           61 g           98 t
ORIGIN      
        1 aatttacgta gctcacgctt aacagagaaa atacttatat atcaaaggct tggttaaaaa
       61 tgattaaaat gcgctaagtc tttgaaatca aaagagaatt taatgattta ctctaatcag
      121 cttcgttaca ctggcaaaga tggtgtttac ttccatctac aaggccaacc agataatttc
      181 ttgtcactat cagcaactga aaacatggaa tttgatagcc ctatgatggt taccactcaa
      241 aatatgcaat cagggcagac tgtaacagat aatgtaatgc gttcacctag aactatttca
      301 atcagtggtg tggttgtggt tggtg
//
LOCUS       DAZPAB010000571          320 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZPAB010000571 DAZPAB010000000
VERSION     DAZPAB010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.580"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
BASE COUNT           88 a           57 c           55 g          120 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt
//
LOCUS       DAZPAB010000572          320 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZPAB010000572 DAZPAB010000000
VERSION     DAZPAB010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.581"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            132..>320
                     /locus_tag="ACHUPG_004568"
     CDS             132..>320
                     /locus_tag="ACHUPG_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863831.1"
                     /translation="MVINFITRDGDDDMNISYVNSNKTTSLPVELDALNNKDISYAKD
                     FFLYIETQLKIAKDFCRPG"
BASE COUNT          118 a           52 c           51 g           99 t
ORIGIN      
        1 cctcggcaaa tgagcgaaat ttgatagaac atttatctgc tgtacgattt aatgaactta
       61 atgatacagc attacaaaaa caatcgaagt ttataaagat gatttctgat tgaccagccc
      121 cctaccctac aatggtgatt aattttatta ctcgcgatgg agatgatgat atgaatatat
      181 catatgtgaa tagcaacaaa acgacatctt tacctgttga gctagatgca ctaaataata
      241 aagatatctc ttatgccaaa gatttctttt tatacataga aacacagtta aaaattgcaa
      301 aagatttttg tagacctgga
//
LOCUS       DAZPAB010000573          311 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZPAB010000573 DAZPAB010000000
VERSION     DAZPAB010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.582"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>311
                     /locus_tag="ACHUPG_004569"
     CDS             <1..>311
                     /locus_tag="ACHUPG_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR0863832.1"
                     /translation="YREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKM
                     AEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRGR
                     A"
BASE COUNT           93 a           54 c           98 g           66 t
ORIGIN      
        1 gatacaggga gataaaacag acgatgcaac tgagcaggct gacgctgaga agtaaaaagc
       61 cggagcttgt ggagcaagag ctgtggggtg tcttactggc ttataatctg gtgagatatc
      121 agatgattaa aatggcggaa catctgaaag gttactggcc gaatcaactg agtttctcag
      181 aatcatgcgg aatggtgatg agaatgctga tgacattgca gggcgcttca ccgggacgta
      241 taccggagct gatgcgcgat cttgcaagta tgggacaact tgtgaaatta ccgacgagaa
      301 ggggaagggc c
//
LOCUS       DAZPAB010000574          309 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZPAB010000574 DAZPAB010000000
VERSION     DAZPAB010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.583"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            110..309
                     /locus_tag="ACHUPG_004570"
                     /pseudo
     CDS             110..309
                     /locus_tag="ACHUPG_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT           84 a           75 c           56 g           94 t
ORIGIN      
        1 ttattcacag cgcgaatgaa aaataaaaca ctctgcttta cagtgtttac tttataatta
       61 acagtcttat ctcactgttc accctgtaac gttcagagag cgtcttcaga tgtctattta
      121 taaaattcct cttccgctca atattctgga agctgcacga gagcgtatta cctagacact
      181 caacacccta ccccgcgttt gtgtctcatt ttctggcggc aaagactctg gtttaatgct
      241 gcatctgaca gccgaacttg cccgacaaat gggcaaaaaa atctgcgttt tgtttatcaa
      301 ctgggaggc
//
LOCUS       DAZPAB010000575          303 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZPAB010000575 DAZPAB010000000
VERSION     DAZPAB010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.584"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
BASE COUNT           92 a           60 c           62 g           89 t
ORIGIN      
        1 aaatggcgga gagaggggga tttgaacccc cggtagagtt gcccctactc cggttttcga
       61 gaccgatgat acagagtcga aaaatcaaag agttaaaaat ttttcttgga aaaaatgtct
      121 taaaatcgta cacgaaaaat caatgaatta cataacatta aagaagaata ttccaagcct
      181 tcttgagtcc accacctgtt ttcaggtgtc attcagacgt gatttgcccc agattgggaa
      241 agcacgcttt tttctcagct tattccgttt tgcctgtgtg ttgaataatt ccactgcagt
      301 tat
//
LOCUS       DAZPAB010000576          300 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZPAB010000576 DAZPAB010000000
VERSION     DAZPAB010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.585"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            139..>300
                     /locus_tag="ACHUPG_004571"
     CDS             139..>300
                     /locus_tag="ACHUPG_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863833.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGIT"
BASE COUNT           92 a           59 c           58 g           91 t
ORIGIN      
        1 atgacgcact gatattattc atcaaataat aacaaaatag ccattgcatc gggttggaca
       61 atttacaaaa gaaagataac ccatattgta taatgcgttt aaatatgccg tcttatataa
      121 aatgatggtc agattaatat gcggattatc tttctacgca aggagtattt atctctactc
      181 ccgtcaatga ttgcatctct tttctctgct aacggtgtcg cggcggccat tgatttatgc
      241 cagggatatg atatcaaagc gagttgtcac gccagcaggc aaagcctttc aggcattacg
//
LOCUS       DAZPAB010000577          297 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZPAB010000577 DAZPAB010000000
VERSION     DAZPAB010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.586"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>297
                     /locus_tag="ACHUPG_004572"
     CDS             <1..>297
                     /locus_tag="ACHUPG_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR0863834.1"
                     /translation="GDKYILTLLNSMRNMEGNKDSRINKNVIVLAGNHEINFNGNYTA
                     RLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGIIRNEENQCYCLGALQVPF"
BASE COUNT          107 a           44 c           51 g           95 t
ORIGIN      
        1 ggtgataaat atatccttac tttgctaaat tctatgcgaa atatggaggg taataaagat
       61 agccgtatta ataaaaatgt catcgttctg gctggaaatc atgaaatcaa ttttaatggt
      121 aactatactg cacgactaga aaattataaa ctctctgccg gtgatacgta taacttaatt
      181 aaaacgctcg atgtttgtaa ttataactca gaaactaaag ttttaaccag tcatcacgga
      241 ataataagaa atgaagaaaa tcaatgttat tgtctcggtg ctttgcaggt tccattt
//
LOCUS       DAZPAB010000578          293 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZPAB010000578 DAZPAB010000000
VERSION     DAZPAB010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.587"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..53
                     /locus_tag="ACHUPG_004573"
     CDS             <1..53
                     /locus_tag="ACHUPG_004573"
                     /inference="COORDINATES: protein motif:HMM:NF037160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0863835.1"
                     /translation="DVTTSLSKVLKNINKD"
     gene            57..293
                     /locus_tag="ACHUPG_004574"
     CDS             57..293
                     /locus_tag="ACHUPG_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFR0863836.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
BASE COUNT          112 a           40 c           43 g           98 t
ORIGIN      
        1 aagatgtaac cacttcccta tcaaaagtat taaagaatat caataaggat taaaatatga
       61 taaaagaaaa aatattatca atagtggcct tttgctatgg tatagcgtac agcaaactat
      121 ctgaagaaac taaatttatt gaagatcttt ctgccgattc tctttctctg attgaaatgc
      181 tggatatgat tagttttgag ttcaatctac gtatagatga atctacatta gaacacatta
      241 ttactattgg agatcttatt tctgtagtca aaaatagtac aaaatcaata tag
//
LOCUS       DAZPAB010000579          292 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZPAB010000579 DAZPAB010000000
VERSION     DAZPAB010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.588"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>292
                     /locus_tag="ACHUPG_004575"
     CDS             <1..>292
                     /locus_tag="ACHUPG_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFR0863837.1"
                     /translation="CFLLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEI
                     IDQKTIFKWDKTPKGMEIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYL"
BASE COUNT          111 a           54 c           50 g           77 t
ORIGIN      
        1 tgttttttac tttacgatgc atccacaaac gctgaaattg ctcaattcaa taaagcaaag
       61 tgtgcaacgc aaatggcacc agattcaact ttcaagatcg cattatcact tatggcattt
      121 gatgcggaaa taatagatca gaaaaccata ttcaaatggg ataaaacccc caaaggaatg
      181 gagatctgga acagcaatca tacaccaaag acgtggatgc aattttctgt tgtttgggtt
      241 tcgcaagaaa taacccaaaa aattggatta aataaaatca agaattatct ca
//
LOCUS       DAZPAB010000580          289 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZPAB010000580 DAZPAB010000000
VERSION     DAZPAB010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.589"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>289
                     /locus_tag="ACHUPG_004576"
     CDS             <1..>289
                     /locus_tag="ACHUPG_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR0863838.1"
                     /translation="TLMDKGYYSLGLLNGWSLAGEHRHWMIPLRKGAQYEELRKLGKG
                     DHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGG"
BASE COUNT           84 a           68 c           89 g           48 t
ORIGIN      
        1 gacgttaatg gataaaggtt attactcact gggactgtta aatggctgga gcctggcggg
       61 agaacaccgc cactggatga tacctctcag aaagggagcg caatatgaag agctcagaaa
      121 actgggtaaa ggcgatcatc tggtgaagct gaaaaccagc ccgcaggcac gaaaaaagtg
      181 gccgggactg ggaaatgaag tgacagcccg cctgctgacc gtgacgcgca aaggaaaagt
      241 ctgccatctg ctgacgtcga tgacggacgc catgcgcttc cccggagga
//
LOCUS       DAZPAB010000581          280 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZPAB010000581 DAZPAB010000000
VERSION     DAZPAB010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.590"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            6..77
                     /gene="ysaE"
                     /locus_tag="ACHUPG_004577"
     CDS             6..77
                     /gene="ysaE"
                     /locus_tag="ACHUPG_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsaE"
                     /protein_id="HFR0863839.1"
                     /translation="MRNAVSKAGIISRRRLLLFQFVG"
     gene            complement(54..206)
                     /gene="hokA"
                     /locus_tag="ACHUPG_004578"
     CDS             complement(54..206)
                     /gene="hokA"
                     /locus_tag="ACHUPG_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR0863840.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
BASE COUNT           86 a           64 c           54 g           76 t
ORIGIN      
        1 tgagaatgcg caacgccgta agtaaggcgg gaataatttc ccgtcgaaga ctcttactct
       61 ttcaatttgt aggctaaaaa cgccgccagc tcataactct cctgtttaat atgcaattca
      121 cacagtgaat ctcttatcat ccaggtgaaa aataaaagcg tgaaacaaat cactattaaa
      181 gaaagtaatc tatatttctg cggcattccc agccccctgt gttgatttcc aacgagtaag
      241 aggctaacct tatggtgttc aggcataggg tagcctcact
//
LOCUS       DAZPAB010000582          279 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZPAB010000582 DAZPAB010000000
VERSION     DAZPAB010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.591"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1..279
                     /locus_tag="ACHUPG_004579"
                     /pseudo
     CDS             1..279
                     /locus_tag="ACHUPG_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          101 a           45 c           51 g           82 t
ORIGIN      
        1 tccggggata tattgattga tatacaatta aataaggatg gcagtctcca gagtaagcac
       61 tacacacttg atgtaggtga agttataaac agttataata ttgaccctct ttgtgaatgc
      121 ttcaagtatt aatcaaactt acttacatga taaaaatcct ggtgacgtta ttagcctgta
      181 tggcacttta gataacattc cccttgtagt taaggaatgc acgggtaaaa atcatcaaat
      241 taatgttaaa gccaaattgc cacaagaacg aagtgaaaa
//
LOCUS       DAZPAB010000583          278 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZPAB010000583 DAZPAB010000000
VERSION     DAZPAB010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.592"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            24..100
                     /locus_tag="ACHUPG_004580"
     tRNA            24..100
                     /locus_tag="ACHUPG_004580"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="ACHUPG_004581"
     tRNA            143..218
                     /locus_tag="ACHUPG_004581"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           75 a           62 c           73 g           68 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaag
//
LOCUS       DAZPAB010000584          277 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZPAB010000584 DAZPAB010000000
VERSION     DAZPAB010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.593"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1..277
                     /locus_tag="ACHUPG_004582"
                     /pseudo
     CDS             1..277
                     /locus_tag="ACHUPG_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT           86 a           58 c           89 g           44 t
ORIGIN      
        1 tgctgggcgc tgaaagaact ggcaaaggat atctggagca gaccatggag cgaggaaaga
       61 cgcaacgact ggcagagatg gctgcggcca acagtgacgt ccccatgatg aagaatgtgg
      121 ctaaaacgat agggaaaagg ttgtacggta tcctgaacgc catgcgccat ggcgtatcaa
      181 acggaaatgc ggaagcactg aacagcaaga tcaggctgct gagaataaaa gccaagggct
      241 accgcaaccg ggagcgcttt aagctgggag tgatgtt
//
LOCUS       DAZPAB010000585          274 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZPAB010000585 DAZPAB010000000
VERSION     DAZPAB010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.594"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            1..274
                     /gene="ydjO"
                     /locus_tag="ACHUPG_004583"
                     /pseudo
     CDS             1..274
                     /gene="ydjO"
                     /locus_tag="ACHUPG_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="protein YdjO"
BASE COUNT           78 a           48 c           55 g           93 t
ORIGIN      
        1 cctgccggga gcatttttgc tgcgattcaa aaaaaatgag agtctcctaa agtttccttc
       61 gataatatca atgcttaata tcaatcttct ttttaatcca ctaaatcttc cgggaatggg
      121 aagtggtgtg cttgaagata ttatgagtat tccagatagt tcacttagaa aacgtctcgg
      181 gtatgaggtt ttaagcttct cactacaggc acattcattg agccaggaat gtattgataa
      241 gttggacatt tttttcgctg acgatctgtt taaa
//
LOCUS       DAZPAB010000586          272 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZPAB010000586 DAZPAB010000000
VERSION     DAZPAB010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.595"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..143)
                     /locus_tag="ACHUPG_004584"
     CDS             complement(<1..143)
                     /locus_tag="ACHUPG_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFR0863841.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFT"
BASE COUNT           94 a           46 c           52 g           80 t
ORIGIN      
        1 gtaaagtctg agaaagcaac gtttgttttg gttgctttca gctttacatc atctgccgca
       61 tgagcagcaa atgcagccat accagatgcc agtatcaaag atatcgaggt acttacgaca
      121 cttttcttta atgcagactt cataaatgtt cctcttctta ttgtacttaa ttccgaagta
      181 attttacgaa gagaaataag tggatgtaag tgaagttagt cacataaaga gatagcagat
      241 ttagctaaaa aaagggaaat aacagtccat aa
//
LOCUS       DAZPAB010000587          270 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZPAB010000587 DAZPAB010000000
VERSION     DAZPAB010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.596"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>270
                     /locus_tag="ACHUPG_004585"
     CDS             <1..>270
                     /locus_tag="ACHUPG_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR0863842.1"
                     /translation="VWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPD
                     ERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLN"
BASE COUNT           68 a           69 c           75 g           58 t
ORIGIN      
        1 gtctggagac tgatgaaacg tgtcggaata aaggctcagg tcggataccg aagcccgcgg
       61 gcacgtaaag gcgaggccag tatcgtgtca cccaacaggc tccagcgaca gttcaatccg
      121 gatgctcctg atgagcgttg ggtaacggac ataacctaca tcaggaccca cgaaggctgg
      181 ctgtatcttg ccgttgttgt tgatctgttc tcacgcaaaa ttatcggctg gtccatgcaa
      241 tcccggatga caaaggacat tgtcctgaac
//
LOCUS       DAZPAB010000588          269 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZPAB010000588 DAZPAB010000000
VERSION     DAZPAB010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.597"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>269
                     /locus_tag="ACHUPG_004586"
     CDS             <1..>269
                     /locus_tag="ACHUPG_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR0863843.1"
                     /translation="SNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFVFSYHVPL
                     FFFAAGFFTIKKNDLSVFDYIKSKFYRLMIPYFTFAFSILIINTI"
BASE COUNT           78 a           40 c           40 g          111 t
ORIGIN      
        1 agcaacagaa aaataagaca tgattgggtt gatgctttaa aatttctcgg tatttttgct
       61 atatatttag gtcaccttgg gttgggggct ggcaaattgt atccttttgt ttttagttat
      121 cacgtcccac tattcttttt cgctgctggt ttttttacta tcaaaaaaaa tgacttatct
      181 gttttcgatt atatcaagtc aaagttttac agattaatga ttccatattt cacttttgca
      241 ttttctatat taataataaa cacaattaa
//
LOCUS       DAZPAB010000589          265 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZPAB010000589 DAZPAB010000000
VERSION     DAZPAB010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.599"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            complement(<1..>265)
                     /gene="rfbG"
                     /locus_tag="ACHUPG_004587"
     CDS             complement(<1..>265)
                     /gene="rfbG"
                     /locus_tag="ACHUPG_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HFR0863844.1"
                     /translation="SFARTRNYSWYIDRKPTMLYRQHENNQVGANISFKAKYKRLGLV
                     RNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILALSFWKLR"
BASE COUNT           74 a           61 c           34 g           96 t
ORIGIN      
        1 gccttaactt ccaaaaactt aaagccaaga tcaaattgcc tatataacct ttacccaatt
       61 gattattcac aaagctatca tcagctaagg cattagctat tttagttact tcttttcgat
      121 accacttgtt tctcaccaaa ccaagccgtt tgtattttgc tttaaaacta atgtttgctc
      181 ccacttggtt gttttcatgc tgtctgtaca gcattgttgg ttttctatca atataccacg
      241 aataatttct cgtccgcgca aatga
//
LOCUS       DAZPAB010000590          258 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZPAB010000590 DAZPAB010000000
VERSION     DAZPAB010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.600"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>258
                     /locus_tag="ACHUPG_004588"
     CDS             <1..>258
                     /locus_tag="ACHUPG_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFR0863845.1"
                     /translation="GKVERMVQYTRNSFYIPLMTRLRPMGSTVDVETANRHGLRWLHD
                     VANQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGEN"
BASE COUNT           67 a           71 c           71 g           49 t
ORIGIN      
        1 ggtaaggtgg aacggatggt gcagtacacc cgtaacagtt tttacatccc actaatgact
       61 cgcctgcgcc cgatggggag cactgtcgat gttgaaacag ccaaccgcca cggtctgcgc
      121 tggctgcacg atgtcgctaa ccaacgaaag catgaaacaa tccaggcccg tccctgtgat
      181 cgctggctcg aagagcagca gtccatgctg gcactgcctc cggagaaaaa agagtatgac
      241 gtgcatcctg gtgaaaat
//
LOCUS       DAZPAB010000591          255 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZPAB010000591 DAZPAB010000000
VERSION     DAZPAB010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.601"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>255
                     /locus_tag="ACHUPG_004589"
     CDS             <1..>255
                     /locus_tag="ACHUPG_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR0863846.1"
                     /translation="SSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLK
                     EAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRA"
BASE COUNT           70 a           62 c           74 g           49 t
ORIGIN      
        1 atcgtccatg gaaacgacat tcgtgctgga tgcactggag caggcgttat gggcccgtcg
       61 accgtccggc acggtccatc acagtgataa aggttctcag tatgtatcgc tggcctacac
      121 acagcggctt aaggaagccg gattactggc atcaacagga agtacaggcg actcgtatga
      181 caacgcgatg gcggagagca tcaatggtct ttacaaagcg gaggtaatac accgtaagag
      241 ctggaaaaac cgtgc
//
LOCUS       DAZPAB010000592          249 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZPAB010000592 DAZPAB010000000
VERSION     DAZPAB010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.602"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
BASE COUNT           64 a           61 c           52 g           72 t
ORIGIN      
        1 cataatacgc tttcccgcta cagagtcaag catttatttt tgctttctct gccggaattc
       61 tcaggagaac cccgctgacc cggcggcgtg tttgccgttg ttccgtgtca gtggtggcgc
      121 attataggga gttattccgg cctgacaagc gaaaaatata aaaactttat cgttcgctca
      181 cttttcaggc aaaacatctt aaatatagtc ttttccgtct aacttataga caaaaacgag
      241 ccccgaagg
//
LOCUS       DAZPAB010000593          248 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZPAB010000593 DAZPAB010000000
VERSION     DAZPAB010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.603"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>248
                     /locus_tag="ACHUPG_004590"
     CDS             <1..>248
                     /locus_tag="ACHUPG_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR0863847.1"
                     /translation="PAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIV
                     VMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIA"
BASE COUNT           52 a           68 c           69 g           59 t
ORIGIN      
        1 ccggcggttt gcttcaaact gaaagatggt gaagatccgg gatacaccct gtacgacctc
       61 tctgaacgtc tgcgtctgcg cggctggcag gttccggcct tcactctcgg cggtgaagcc
      121 accgacatcg tggtgatgcg cattatgtgt cgtcgcggct tcgaaatgga ctttgctgaa
      181 ctgttgctgg aagactacaa agcctccctg aaatatctca gcgatcaccc gaaactgcag
      241 ggtattgc
//
LOCUS       DAZPAB010000594          248 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZPAB010000594 DAZPAB010000000
VERSION     DAZPAB010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.604"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>248
                     /locus_tag="ACHUPG_004591"
     CDS             <1..>248
                     /locus_tag="ACHUPG_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0863848.1"
                     /translation="GKDTHHAVAINRSGKRLFDKALPNDENKLRSLISDLKQHGQILL
                     VVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLH"
BASE COUNT           63 a           71 c           57 g           57 t
ORIGIN      
        1 cggtaaagat acgcatcacg ctgttgccat taatcgttca ggtaaacgcc tgttcgataa
       61 agcattaccc aacgacgaaa acaaactcag gtcgctaata tctgacctga aacaacatgg
      121 tcagatactg ctggttgttg atcagccagc taccatcggt gcgttacctg tcgccgttgc
      181 ccgctcagaa ggagtccttg tcggatacct ccctggactg gccatgcgcc gcatagccga
      241 cttacacg
//
LOCUS       DAZPAB010000595          243 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZPAB010000595 DAZPAB010000000
VERSION     DAZPAB010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.605"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..>243
                     /locus_tag="ACHUPG_004592"
     CDS             <1..>243
                     /locus_tag="ACHUPG_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFR0863849.1"
                     /translation="RGGMTILRAFIRSLSVPQEQEPAVRFETEPGRQMQVDWGTMRNG
                     RSPLHVFVAVPGYSRMLYIEFTDNMRYDTLETCHRNA"
BASE COUNT           50 a           65 c           66 g           62 t
ORIGIN      
        1 cgtggcggaa tgaccattct cagggcattc attcgttctc tctcggttcc tcaggagcag
       61 gagcctgccg ttcggttcga aactgaaccc ggacgacaga tgcaggttga ctggggcact
      121 atgcgtaatg gtcgctcacc gcttcacgtg ttcgttgctg ttcccggata cagccgaatg
      181 ttgtacatcg aattcactga caatatgcgt tatgacacgc tggagacctg ccatcgtaat
      241 gcg
//
LOCUS       DAZPAB010000596          243 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZPAB010000596 DAZPAB010000000
VERSION     DAZPAB010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.606"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
BASE COUNT           83 a           53 c           27 g           80 t
ORIGIN      
        1 ttttctgctc aacagacaca aacaggcaat aatactctcc aaagcgaaca atatttggca
       61 ttacgattta atgcttcata atcaccaaat tatttaacca taaattttct tatttctaac
      121 aaaatgattg gtgtgttgtt ggagttaaaa tacatacata atattaacat ctgccaacat
      181 ttcactttcc gcaagacgga tgatttcgaa tcctcatcca cgttacccta tcccaaatat
      241 ctt
//
LOCUS       DAZPAB010000597          241 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZPAB010000597 DAZPAB010000000
VERSION     DAZPAB010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.denovo.607"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..230
                     /locus_tag="ACHUPG_004593"
     CDS             <1..230
                     /locus_tag="ACHUPG_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFR0863850.1"
                     /translation="GENALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEP
                     EDWLREVIEKLNDWPSNQVHELLPWNFSSVK"
BASE COUNT           66 a           53 c           69 g           53 t
ORIGIN      
        1 tcggtgaaaa cgcgttacga tcggtggcgg ttggaagaaa aaattatctc tttttcggct
       61 cagacaaggg aggagaaagt gcggcgatca tctacagtct gctggtcacc tgcaaacaga
      121 acgaagtgga gccggaggac tggttgcgcg aagtgatcga gaagctcaat gactggccgt
      181 cgaaccaagt gcatgaactg ctgccctgga acttctcgtc tgtaaaataa tccttacgct
      241 a
//
LOCUS       DAZPAB010000598         5646 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.guided.219, whole genome shotgun sequence.
ACCESSION   DAZPAB010000598 DAZPAB010000000
VERSION     DAZPAB010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.guided.219"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            59..1102
                     /locus_tag="ACHUPG_004594"
     CDS             59..1102
                     /locus_tag="ACHUPG_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR0863851.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            1164..1778
                     /gene="prfH"
                     /locus_tag="ACHUPG_004595"
                     /pseudo
     CDS             1164..1778
                     /gene="prfH"
                     /locus_tag="ACHUPG_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="ACHUPG_004596"
     CDS             complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="ACHUPG_004596"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR0863852.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3554..4012
                     /gene="gpt"
                     /locus_tag="ACHUPG_004597"
     CDS             3554..4012
                     /gene="gpt"
                     /locus_tag="ACHUPG_004597"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR0863853.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4104..5348
                     /gene="frsA"
                     /locus_tag="ACHUPG_004598"
     CDS             4104..5348
                     /gene="frsA"
                     /locus_tag="ACHUPG_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR0863854.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5406..>5646
                     /gene="crl"
                     /locus_tag="ACHUPG_004599"
     CDS             5406..>5646
                     /gene="crl"
                     /locus_tag="ACHUPG_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR0863855.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAG"
BASE COUNT         1346 a         1405 c         1518 g         1377 t
ORIGIN      
        1 aattcaagtc gcagcacatg caacttgaag tatgacgagt ctatttacgg aatggtatat
       61 gggcaattat attcgtccct tatccgatgc ggtatttacc atcgcatctg atgacctgtg
      121 gatcgagagt ttagcgatcc aacaattaca caccacggca aatttaccca acatgcagcg
      181 cgtagtgggg atgccagatt tacaccccgg acgcggctac ccgattggcg cagcgttctt
      241 ctccgcaggc cgtttttacc cagcactggt cggcaatgat atcggctgtg gcatggcgct
      301 atggcaaaca gatattctcg ctcgcaaata caacgccgat aagtttgaaa agcgattatc
      361 agctttggat gacgttgctg aagaaagctg gctggaggaa aacctgccgt cagcatttgc
      421 acagcatccg tggcgcagct cgcttggctc tatcggtggt ggcaatcatt tcgctgaact
      481 gcaacaagtt gatcaaatca tcaacgctga actgtttgca ctggcaggtc tggatgcgca
      541 gcatctgcaa ctgctggttc atagcggctc gagggggcta gggcagtcta ttttacagcg
      601 gcatattgcc tcgttttcgc atcatggttt gcctgaaggc agtgacgacg cgctggcgtt
      661 tgcgcgtttt aatcgccacc tgatcgcttt gcgcataatg caacaggtta aggccaccgg
      721 aagtccggtt ctggatgtgg cgcataactt tgttagcgcg tgtcgaatcg gtgatcaaca
      781 aggggtctta catcgtaaag gtgccacgcc tgatgactgt ggtctggtgg ttattcccgg
      841 ttcacgcggt gattactcca ggctggttca gcccgtcagg agtgaggaaa cattgcattc
      901 gctggcgcat ggggctgggc gtaaatgggg gcgcaccgag tgtaaagggc gtctggcagc
      961 gaaatacaca gcgacgcaac tctcgcgtac tgaacttggc agtcgggtaa tttgtcgcga
     1021 taaacaactc atcttcgaag aagcgccaca agcctataaa tcggctgaaa gtgtggtgca
     1081 atgtcagggc aggccggatt aattattcct gtcgcgcggc tgcgtccggt gctaacgctc
     1141 aaaaacagtg gagggaaaaa aggatgatct tgctataact ctcctcagcg caggggccgg
     1201 aagaatgttg tctcgcagtg aaaaaggcgc tggacaggct aattaaagaa gctgcccgac
     1261 aagacgtcgc ggtaacggtg ctggaaacag aaacaggccg ctactctgac acgctgcgtt
     1321 cggcgctgat ttctctggat ggcgacaacg catgggcgtt aagcgaaagc tggtgtggca
     1381 ctattcagtg gatttgcccg agcccgtatc gcccacatca cgggcgtaaa aactggtttc
     1441 tgggcattgg gcgttttacc gctgatgagc aggagcaatc cgatgcgatc cggtatgaga
     1501 cgctgcgttc gtcggggccg ggcggtcaac atgtcaataa aaccgactcg gcggtacgcg
     1561 ccacgcattt ggcatccggt attagcgtga aggttcagtc agagcgcagc cagcatgcta
     1621 acaaacggct ggcgcgattg ctgattgcct ggaagctgga tcagcagcaa taggaaaata
     1681 gcgcggtgct gaaatcacag cggcgaatgt tccatcacca gattgaacgt ggcaacccgc
     1741 gacgaacgtt tacagggatg gcttttatcg aaggataatg aaggatgaaa ctgccggaag
     1801 gcgattaaac gccatccggc agcaaatcaa ataattactt cgccggaatt tctttcagca
     1861 gttcagtcag cagtgtccag taatgaccta cgctttcgat gtgaacttgc tcatccggag
     1921 agtgtggacc ggtgatagtt ggcccgatag aaaccatgtc catttccgga tacggttttt
     1981 tgaacagacc acattccagg cccgcgtgga taatctggat gttcggcgtc ttgttgaaca
     2041 ggcgctgata ggtttcacgt accagatgca tcaccggaga attagcgtcc ggctgccagc
     2101 caggatatgc gcctttcgct tcggttttcg cgccagccag tttacccagc gaatccagca
     2161 tgctcaccac gtagtcttta ccgctgtcga ttagtgaacg gatcaggcag tgaatttcta
     2221 cgttattgtc agtcatggtc accacaccga cgttcaggga ggtttcgacc acaccttttg
     2281 ccacgtcgga attgcggatc acaccgttcg gggtggcgtt cagcagacga ataaaggtat
     2341 cgcgagattt cgcaatcagg gcagctttat cgttcgctac agagtccagc aacaaggcca
     2401 gatttttctc tttctctgcc agctcgtttt tcaggatctc ctgataggta ttcaccagag
     2461 atttcagtgc gtcgacttta tcagctgcga cagcaatggt cgcaaaggct tcacgcggga
     2521 tggcgttacg cagtgtgccg ccgttgaaat cgataaggcg caggtccagt tcttctgcat
     2581 gacccgccag gaagcgcacc agcagtttgt tggcattacc caggccaacg tggatttcac
     2641 cgccggagtg accgcctttc agacctttta aggttaactt gaaggtttca aaaccagctg
     2701 gaaccgcttc acgatctaaa tgcaggttgg aggtgaagtc gatacccccc gcacaaccca
     2761 tgtagatttc accttcttct tcggagtcgg tgttaatcag aatatcagcc tgcaaccagt
     2821 tgctctgtaa gccgaacgca ccgtccatac cggcttcttc ggtcatggtc agcagcactt
     2881 ccagcgggcc gtgaaccacg ttttcgtcag ccagaaccgc cagtgcagag gccataccaa
     2941 tgccgttatc cgcacccagc gtggtgccgc gcgctttaac ccattcgcca tcaatataag
     3001 gctggatagg atctttcgtg aagtcatgca cggtgtcgtt atttttctgc ggcaccatat
     3061 cgaggtgggc ctgtaagacg accggtttac gattttccat acctgcggta gcaggttgac
     3121 gaatcaggat attacctacc tgatcgcgtt cgacatggaa acctttctct tttgcccaac
     3181 caacaatgta ttcagcgagt tgctcttcat gataggacgg gtgaggaata gaacagattt
     3241 tggcaaaaat atcccacagc ggctgtggag ataattgaga cagttcagac acgttaagtc
     3301 tccttgtcga tcacccgcaa aacagtattg caggtcacag ggttagcaga aaatgttgtc
     3361 aacacaagac aggcttgcga gatatgtatt gagaatacca ctttatcccg cgtcagggag
     3421 aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct
     3481 ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc
     3541 tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc
     3601 acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag
     3661 ccgtggcggt ctggtaccgg gggcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     3721 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
     3781 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
     3841 tgctgttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     3901 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggatcga
     3961 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     4021 acgcctggca ttgccgggcg ttgttctttt taacttcagg caggttacaa tagtttccag
     4081 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     4141 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     4201 cgcctgtgca gtcggctctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     4261 gtctgatgtg gatctggcgc ggtattgacc cacgtgaaat cctcgacgtc caggcacgta
     4321 ttgtgatgag cgatgctgaa cgtaccgacg atgatttata cgataccgtg attggctacc
     4381 gtggtggcaa ctggatttat gagtgggcca cccaggcgat ggtgtggcaa caaaaagcct
     4441 gtacggaaga agatccgcaa ctcagtggtc gacactggct gcatgcggct acgttgtaca
     4501 acattgccgc ctatcctcat ctgaaaggag atgacctggc cgagcaagcg caggctttat
     4561 caaaccgcgc ctatgaagag gccgcccagc gtctgccggg cacgatgcgg cagatggagt
     4621 ttaccgtacc cggcggtgcg cccatcaccg gctttttgca tatgccgaaa ggcgatggcc
     4681 cgttcccgac agtattaatg tgtggtggtc tggatgcgat gcagacggac tattacagcc
     4741 tgtatgaacg ttattttgcg ccgcgcggca ttgcgatgct gactattgat atgccgtcgg
     4801 tgggcttttc ttcaaaatgg aagctcaccc aggactccag cctgttgcat cagcacgtct
     4861 taaaggcgct gcctaacgta ccgtgggtgg atcacactcg cgtcgcggcc tttggtttcc
     4921 gtttcggcgc taacgttgcc gtgcgtctgg cataccttga atcgccgcgt ctgaaagcgg
     4981 ttgcctgtct tggtccggta gttcataccc tgttgagtga ttttaagtgc cagcaacagg
     5041 tgccggaaat gtatcttgac gttctggcga gtcgtttggg gatgcatgat gcttccgacg
     5101 aagcgttacg cgtggagctg aatcgctatt cattaaaagt gcaaggattg ctggggcgtc
     5161 gctgcccaac gccaatgtta tcaggctact ggaagaacga tccgttcagc ccggaagagg
     5221 actcacgctt aatcacctca tcatctgctg acggtaaatt attagagatc ccatttaacc
     5281 cggtgtatcg gaattttgac aaaggtcttc aggaaatcac cggctggatc gaaaaacgct
     5341 tgtgttaaaa atttgctaaa ttttgccaat ttggtaaaac agttgcatca caacaggaga
     5401 tagcaatgac gttaccgagt ggacacccga agagcagatt gatcaaaaaa tttaccgcac
     5461 taggcccgta tattcgtgaa ggtaagtgcg aagataatcg attctttttc gattgtctgg
     5521 ctgtatgcgt caacgtgaaa ccggcaccgg aagtgcgtga attctggggc tggtggatgg
     5581 agctggaagc gcaggaatcc cgttttacct acagctacca gtttggtctg ttcgataaag
     5641 caggcg
//
LOCUS       DAZPAB010000599         2976 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.guided.304, whole genome shotgun sequence.
ACCESSION   DAZPAB010000599 DAZPAB010000000
VERSION     DAZPAB010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.guided.304"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            41..430
                     /locus_tag="ACHUPG_004600"
                     /pseudo
     CDS             41..430
                     /locus_tag="ACHUPG_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(408..1031)
                     /gene="tetR(B)"
                     /locus_tag="ACHUPG_004601"
     CDS             complement(408..1031)
                     /gene="tetR(B)"
                     /locus_tag="ACHUPG_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFR0863856.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1113..2318
                     /gene="tet(B)"
                     /locus_tag="ACHUPG_004602"
     CDS             1113..2318
                     /gene="tet(B)"
                     /locus_tag="ACHUPG_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFR0863857.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2431..>2976)
                     /locus_tag="ACHUPG_004603"
     CDS             complement(2431..>2976)
                     /locus_tag="ACHUPG_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFR0863858.1"
                     /translation="SLVNSARILFVEKGYQAVSIDEISGKALVTKGAFYHHFKNKKQL
                     LSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIIDNNKNLIPIQEVMPIIG
                     WNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLNGWFMHIAIHAKNLKEL
                     ADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          789 a          569 c          613 g         1005 t
ORIGIN      
        1 tctgaacata aaacactatc aataagttgg agtcattacc aaccatggcg tacatgccaa
       61 agtttccacg ccagtgcatt tacgaaaagc acgtacttgc tatgatcatt tagctggcga
      121 agttgccgtt aagatctatg attccctttg tcaacagcaa tggatcactg aaaatggttc
      181 aatgatcaca ttaagtggta ttcaatattt tcatgaaatg ggaattgacg ttccttccaa
      241 acattcacgt aaaatctgtt gtgcgtgttt agattggagt gaacgccgtt tccatttagg
      301 tgggtacgtt ggagccgcat tattttcgct ttatgaatct aaagggtggt taactcgaca
      361 tcttggttac cgtgaagtta ccatcacgga aaaaggttat gctgctttta agacccactt
      421 tcacatttaa gttgtttttc taatccgcat atgatcaatt caaggccgaa taagaaggct
      481 ggctctgcac cttggtgatc aaataattcg atagcttgtc gtaataatgg cggcatacta
      541 tcagtagtag gtgtttccct ttcttcttta gcgacttgat gctcttgatc ttccaatacg
      601 caacctaaag taaaatgccc cacagcgctg agtgcatata atgcattctc tagtgaaaaa
      661 ccttgttggc ataaaaaggc taattgattt tcgagagttt catactgttt ttctgtaggc
      721 cgtgtaccta aatgtacttt tgctccatcg cgatgactta gtaaagcaca tctaaaactt
      781 ttagcgttat tacgtaaaaa atcttgccag ctttcccctt ctaaagggca aaagtgagta
      841 tggtgcctat ctaacatctc aatggctaag gcgtcgagca aagcccgctt attttttaca
      901 tgccaataca atgtaggctg ctctacacct agcttctggg cgagtttacg ggttgttaaa
      961 ccttcgattc cgacctcatt aagcagctct aatgcgctgt taatcacttt acttttatct
     1021 aatctagaca tcattaattc ctaatttttg ttgacactct atcattgata gagttatttt
     1081 accactccct atcagtgata gagaaaagtg aaatgaatag ttcgacaaag atcgcattgg
     1141 taattacgtt actcgatgcc atggggattg gccttatcat gccagtcttg ccaacgttat
     1201 tacgtgaatt tattgcttcg gaagatatcg ctaaccactt tggcgtattg cttgcacttt
     1261 atgcgttaat gcaggttatc tttgctcctt ggcttggaaa aatgtctgac cgatttggtc
     1321 ggcgcccagt gctgttgttg tcattaatag gcgcatcgct ggattactta ttgctggctt
     1381 tttcaagtgc gctttggatg ctgtatttag gccgtttgct ttcagggatc acaggagcta
     1441 ctggggctgt cgcggcatcg gtcattgccg ataccacctc agcttctcaa cgcgtgaagt
     1501 ggttcggttg gttaggggca agttttgggc ttggtttaat agcggggcct attattggtg
     1561 gttttgcagg agagatttca ccgcatagtc ccttttttat cgctgcgttg ctaaatattg
     1621 tcgctttcct tgtggttatg ttttggttcc gtgaaaccaa aaatacacgt gataatacag
     1681 ataccgaagt aggggttgag acgcaatcga attcggtata catcacttta tttaaaacga
     1741 tgcccatttt gttgattatt tatttttcag cgcaattgat aggccaaatt cccgcaacgg
     1801 tgtgggtgct atttaccgaa aatcgttttg gatggaatag catgatggtt ggcttttcat
     1861 tagcgggtct tggtctttta cactcagtat tccaagcctt tgtggcagga agaatagcca
     1921 ctaaatgggg cgaaaaaacg gcagtactgc tcggatttat tgcagatagt agtgcatttg
     1981 cctttttagc gtttatatct gaaggttggt tagttttccc tgttttaatt ttattggctg
     2041 gtggtgggat cgctttacct gcattacagg gagtgatgtc tatccaaaca aagagtcatc
     2101 agcaaggtgc tttacaggga ttattggtga gccttaccaa tgcaaccggt gttattggcc
     2161 cattactgtt tgctgttatt tataatcatt cactaccaat ttgggatggc tggatttgga
     2221 ttattggttt agcgttttac tgtattatta tcctgctatc gatgaccttc atgttaaccc
     2281 ctcaagctca ggggagtaaa caggagacaa gtgcttagtt atttcgtcac caaatgatgt
     2341 tattccgcga aatataatga ccctcttgat aacccaagag ggcatttttt acgataaaga
     2401 agatttagct tcaaataaaa cctatctatt ttatttatct ttcaagctca ataaaaagcc
     2461 gcggtaaata gcaataaatt ggcctttttt atcggcaagc tcttttaggt ttttcgcatg
     2521 tattgcgata tgcataaacc agccattgag taagttttta agcacatcat catcataagc
     2581 tttaagttgg ttctcttgga tcaatttgct gacaatggcg tttaccttac cagtaatgta
     2641 ttcaaggcta attttttcaa gttcattcca accaatgata ggcatcactt cttggatagg
     2701 gataaggttt ttattattat caataatata atcaagataa tgttcaaata tactttctaa
     2761 ggcagaccaa ccatttgtta aatcagtttt tgttgtgatg taggcatcaa tcataattaa
     2821 ttgctgctta taacaggcac tgagtaattg ttttttattt ttaaagtgat gataaaaggc
     2881 acctttggtc accaacgctt ttcccgagat ctcatctatt gaaacagctt gatagccttt
     2941 ttcaacaaac aatattcgtg ctgagttaac cagtga
//
LOCUS       DAZPAB010000600         2681 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.guided.325, whole genome shotgun sequence.
ACCESSION   DAZPAB010000600 DAZPAB010000000
VERSION     DAZPAB010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.guided.325"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            263..922
                     /locus_tag="ACHUPG_004604"
     CDS             263..922
                     /locus_tag="ACHUPG_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFR0863859.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1123..1500)
                     /locus_tag="ACHUPG_004605"
     CDS             complement(1123..1500)
                     /locus_tag="ACHUPG_004605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0863860.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1567..>2681)
                     /locus_tag="ACHUPG_004606"
     CDS             complement(1567..>2681)
                     /locus_tag="ACHUPG_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFR0863861.1"
                     /translation="INLGLTKMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQ
                     FRHPFAGHWGDGTTSSSDGQNFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFH
                     TKVVNVGLRDSTYVLDGLLYHESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRD
                     LGDTKLYIPKGDAAYDALKPMIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRK
                     LGSYPRQNGLAVALRELGRIERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFF
                     NRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYL
                     SPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
BASE COUNT          610 a          678 c          747 g          646 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata
       61 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      121 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
      181 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      241 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      301 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      361 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      421 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      481 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      541 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      601 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      661 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      721 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      781 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      841 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      901 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
      961 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc
     1021 gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt
     1081 accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag
     1141 tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc
     1201 cagagcctga taaaaacggt tagcgccggg gttggatttt tcagcgttcc agctaaggct
     1261 aaggcattcc tgttcaagcg caagccgggc tataaagcgc attatcgctt tacccgtgcc
     1321 cttatttcga tcgcactgag aaacatacag ctctttaata tgcagctgac cgctgtatcg
     1381 gggcgaggga taaagaatat tgcaacatgc caggccagta atgttattgc cgcagcgcgc
     1441 tctgatcacc agggtaccgg aaagccggtt gaataacttt ttacagagat aatccttcat
     1501 caacgcttcc tgaatgatac cttcgccata ataggggtcg tctcagaaaa cggaaaataa
     1561 agcacgctaa gccggttgca gaggccgtag cggcctgaac ttccccgcgc cgatcttggc
     1621 gctgctgcgc cataggtaat caccggtcag gttgatgtgc tcccagccga gtggcgacag
     1681 gtactgcaat agcgagtcat cgacggcatg accattgccg cgcaacgcat gcgccgcacg
     1741 ctccaggtag accgtgttcc acagcacgat ggccgccgtc accaggttga ggccgctggc
     1801 ccggtagcgc tgctgctcga aactgcggtc acggatttca ccaaggcggt tgaagaacac
     1861 ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc ccggcatgca cgcggcggcg
     1921 tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg cgctcgatgc ggcccaactc
     1981 gcgcagcgcg acggccaagc cgttctggcg cgggtagctg ccgagtttcc tgagcatcag
     2041 cgaggccgtc accgtgccct gcttgatcga ggtggccagc cgcaggattt cgtcccaatg
     2101 ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatc ggcttgagcg cgtcataggc
     2161 ggcatcgccc ttcgggatgt agagcttggt gtcgcccagg tcgcggatgc gcggcgcgaa
     2221 gcggaagccc aagaggtgca tcagggcgaa gacgtgatcg gtgaagcccg ccgtgtcggt
     2281 gtagtgctcc tcgatccgca ggtcggattc gtggtacagc aggccgtcga gcacgtaggt
     2341 tgagtcgcgc aggccgacat tgaccacctt ggtgtggaat ggcgcgtatt ggtcggagat
     2401 gtgggtgtag aaagtccgtc ctgggctgct gccatatttt gggttgatgt gccccgtgct
     2461 ctttgccttg ctagcggttc ggaaattctg tccgtccgat gatgatgtgg tgccatcgcc
     2521 ccagtgcccg gcaaagggat gccgaaactg agcgttgacc agttcagcca acgctgtcga
     2581 gtacgtttcg tcgcgggtat gccaggcttg cagccaagcg agcttcgcgt aggtcgtgcc
     2641 ggggcaggac tcggccatct tggtcaggcc caggttgatc g
//
LOCUS       DAZPAB010000601         2140 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.guided.357, whole genome shotgun sequence.
ACCESSION   DAZPAB010000601 DAZPAB010000000
VERSION     DAZPAB010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.guided.357"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            26..1158
                     /locus_tag="ACHUPG_004607"
                     /pseudo
     CDS             26..1158
                     /locus_tag="ACHUPG_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            1247..1780
                     /gene="blc"
                     /locus_tag="ACHUPG_004608"
     CDS             1247..1780
                     /gene="blc"
                     /locus_tag="ACHUPG_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR0863862.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1777..2094)
                     /gene="sugE"
                     /locus_tag="ACHUPG_004609"
     CDS             complement(1777..2094)
                     /gene="sugE"
                     /locus_tag="ACHUPG_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR0863863.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
BASE COUNT          546 a          566 c          535 g          493 t
ORIGIN      
        1 ccgttttgta tggaaaccag accttatgtt caaaacgacg ctctgcgcct tattaattac
       61 cgcctcttac tccacatttg ctgcccctca acaaatcaac gatattgtgc atcgcacaat
      121 taccccgctt atagagcaac aaaagatccc cggtatggcg gtggcggtaa tttatcaggg
      181 taaaccttat tactttacct ggggctatgc ggacatcgcc aaaaagcagc ccgtcacaca
      241 gcaaacgttg tttgagttag gttcggtcag caaaacattt acgggcgtgc ttggtggcga
      301 cgctattgct cgaggggaaa tcaagttaag cgatcccgca acaaaatact ggcctgaact
      361 taccgctaaa cagtggaatg ggatcacact attacatctt gcgacctaca ccgctggcgg
      421 cctgccattg caggtgccgg atgaagtgaa atcctcaagc gacttgctgc gcttctatca
      481 aaactggcag cctgcatggg ctccaggaac acaacgtctg tatgccaact ccagtatcgg
      541 tttgttcggc gcactggctg tgaagccgtc tggtttgagt tttgagcagg cgatgcaaac
      601 tcgtgtcttc cagccactca aactcaacca tacgtggatt aatgtacctc ccccagaaga
      661 aaagaattac gcctggggat atcgcgaagg taaggcagtg catgtttcgc caggggcgtt
      721 agatgctgaa gcttatggtg tgaagtcgac cattgaagat atggcccgct gggtgcaaag
      781 caatttaccc cccttgatat caatgagaaa acgcttcaac aagggataca actggcacaa
      841 tctcgctact ggcaaaccgg cgatatgtat cagggcctgg gctgggaaat gctggactgg
      901 ccggtcaatc ctgacagcat cattaacggc agtgacaata aaattgcact ggcagcacgc
      961 ccggtaaaag cgattaagcc cccaactcct gcagtacgcg catcatgggt acataaaacg
     1021 ggggcgaccg gcggatttgg tagctatgtc gcgtttattc cagaaaaaga gctgggtatc
     1081 gtgatgctgg ctaacaaaaa ctatcccaat ccagcgagag tcgccgccgc ctgtcagatt
     1141 ctcaacgctc tacagtaaaa tttcatcggg tccgaatttt cggacctttt ctgcgctttt
     1201 ccttgctgtc atctacactt agaaaaaaac cagtaaggaa acatttatgc gcctgctccc
     1261 tctcgttgcc gcagcgacag ctgcatttct ggtcgttgcc tgtagttttc ctacgccgcc
     1321 gcgtggcgtg accgtagtga ataatttcga cgccaaacgt tatctcggta cctggtatga
     1381 gattgcccgt tttgatcacc gttttgaacg tggactggaa aaagtcaccg caacatacag
     1441 cctgcgtgat gacggcggcc tgaatgtcat taataaaggc tataaccctg acagaggaat
     1501 gtggcagcag agtgaaggga aagcgtactt taccggcgca ccaactcgcg ctgcgctgaa
     1561 agtgtcattc tttggtcctt tctatggcgg ttataacgtt attgcactcg atcgggaata
     1621 ccgccatgcg ctggtttgcg gcccggaccg cgactacctg tggatactct cccgcacgcc
     1681 aaccatttct gacgaagtga aacaggagat gctggcagtc gcgacccggg aagggtttga
     1741 tgtcagtaag tttatttggg tacagcagcc cggtagttag tgagtgctga gtttcagacc
     1801 aataatcccc aatacgatta acgccagact cgccagacgc atcgggttag cggactcacc
     1861 gagcagcaca atgccggtga tggccgcgcc gactgcgcca atacccgtcc acacggcata
     1921 agccgtccct accggtaacg atttcatcgc ccaggcaagt agcgccatac tgactatcat
     1981 cgccgtcacg gtaataacac tcggcgtcaa acggctaaag ccgtgggtat atttcaggcc
     2041 aacggcccat accacttcca gcagaccagc aataactaag ataatccagg acatatcagg
     2101 ctccggaaat ttggggccgt ccccggtgaa aagaggcgtt
//
LOCUS       DAZPAB010000602         1751 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.guided.387, whole genome shotgun sequence.
ACCESSION   DAZPAB010000602 DAZPAB010000000
VERSION     DAZPAB010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.guided.387"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..223
                     /locus_tag="ACHUPG_004610"
     CDS             <1..223
                     /locus_tag="ACHUPG_004610"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFR0863864.1"
                     /translation="TNLKKITDHVIVSGGGEIYKSLIDQVDTLHISTIDIEPEGDVYF
                     PEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            318..842
                     /gene="sat2"
                     /locus_tag="ACHUPG_004611"
     CDS             318..842
                     /gene="sat2"
                     /locus_tag="ACHUPG_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR0863865.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            900..1738
                     /gene="aadA1"
                     /locus_tag="ACHUPG_004612"
                     /pseudo
     CDS             900..1738
                     /gene="aadA1"
                     /locus_tag="ACHUPG_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT          468 a          427 c          448 g          408 t
ORIGIN      
        1 aaccaaccta aagaaaataa cggatcatgt cattgtttca ggtggtgggg agatatacaa
       61 aagcctgatc gatcaagtag atacactaca tatatctaca atagacatcg agccggaagg
      121 tgatgtttac tttcctgaaa tccccagcaa ttttaggcca gtttttaccc aagacttcgc
      181 ctctaacata aattatagtt accaaatctg gcaaaagggt taacaagtgg cagcaacgga
      241 ttcgcaaacc tgtcacgcct tttgtaccaa aagccgcgcc aggtttgcga tccgctgtgc
      301 caggcgttag gcgtcatatg aagatttcgg tgatccctga gcaggtggcg gaaacattgg
      361 atgctgagaa ccatttcatt gttcgtgaag tgttcgatgt gcacctatcc gaccaaggct
      421 ttgaactatc taccagaagt gtgagcccct accggaagga ttacatctcg gatgatgact
      481 ctgatgaaga ctctgcttgc tatggcgcat tcatcgacca agagcttgtc gggaagattg
      541 aactcaactc aacatggaac gatctagcct ctatcgaaca cattgttgtg tcgcacacgc
      601 accgaggcaa aggagtcgcg cacagtctca tcgaatttgc gaaaaagtgg gcactaagca
      661 gacagctcct tggcatacga ttagagacac aaacgaacaa tgtacctgcc tgcaatttgt
      721 acgcaaaatg tggctttact ctcggcggca ttgacctgtt cacgtataaa actagacctc
      781 aagtctcgaa cgaaacagcg atgtactggt actggttctc gggagcacag gatgacgcct
      841 aacaattcat tcaagccgac accgcttcgc ggcgcggctt aattcaggag ttaaacatca
      901 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
      961 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     1021 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     1081 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     1141 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     1201 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     1261 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     1321 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     1381 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     1441 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     1501 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     1561 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     1621 cgcgcgcaga tcagttggaa gaatttgttc actacgtgaa aggcgagatc accaaggtag
     1681 tcggcaaata atgtctaaca attcgttcaa gccgacgccg cttcgcggcg cggcttaact
     1741 caagcgttag a
//
LOCUS       DAZPAB010000603         1748 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.guided.389, whole genome shotgun sequence.
ACCESSION   DAZPAB010000603 DAZPAB010000000
VERSION     DAZPAB010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.guided.389"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..223
                     /locus_tag="ACHUPG_004613"
     CDS             <1..223
                     /locus_tag="ACHUPG_004613"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFR0863866.1"
                     /translation="TNLKKITDHVIVSGGGEIYKSLIDQVDTLHISTIDIEPEGDVYF
                     PEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            318..842
                     /gene="sat2"
                     /locus_tag="ACHUPG_004614"
     CDS             318..842
                     /gene="sat2"
                     /locus_tag="ACHUPG_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR0863867.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            900..1735
                     /gene="aadA1"
                     /locus_tag="ACHUPG_004615"
                     /pseudo
     CDS             900..1735
                     /gene="aadA1"
                     /locus_tag="ACHUPG_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT          465 a          427 c          448 g          408 t
ORIGIN      
        1 aaccaaccta aagaaaataa cggatcatgt cattgtttca ggtggtgggg agatatacaa
       61 aagcctgatc gatcaagtag atacactaca tatatctaca atagacatcg agccggaagg
      121 tgatgtttac tttcctgaaa tccccagcaa ttttaggcca gtttttaccc aagacttcgc
      181 ctctaacata aattatagtt accaaatctg gcaaaagggt taacaagtgg cagcaacgga
      241 ttcgcaaacc tgtcacgcct tttgtaccaa aagccgcgcc aggtttgcga tccgctgtgc
      301 caggcgttag gcgtcatatg aagatttcgg tgatccctga gcaggtggcg gaaacattgg
      361 atgctgagaa ccatttcatt gttcgtgaag tgttcgatgt gcacctatcc gaccaaggct
      421 ttgaactatc taccagaagt gtgagcccct accggaagga ttacatctcg gatgatgact
      481 ctgatgaaga ctctgcttgc tatggcgcat tcatcgacca agagcttgtc gggaagattg
      541 aactcaactc aacatggaac gatctagcct ctatcgaaca cattgttgtg tcgcacacgc
      601 accgaggcaa aggagtcgcg cacagtctca tcgaatttgc gaaaaagtgg gcactaagca
      661 gacagctcct tggcatacga ttagagacac aaacgaacaa tgtacctgcc tgcaatttgt
      721 acgcaaaatg tggctttact ctcggcggca ttgacctgtt cacgtataaa actagacctc
      781 aagtctcgaa cgaaacagcg atgtactggt actggttctc gggagcacag gatgacgcct
      841 aacaattcat tcaagccgac accgcttcgc ggcgcggctt aattcaggag ttaaacatca
      901 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
      961 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     1021 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     1081 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     1141 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     1201 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     1261 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     1321 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     1381 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     1441 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     1501 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     1561 agcccgtcat acttgaagct aggcaggctt atcttggaca agaagatcgc ttggcctcgc
     1621 gcgcagatca gttggaagaa tttgttcact acgtgaaagg cgagatcacc aaggtagtcg
     1681 gcaaataatg tctaacaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa
     1741 gcgttaga
//
LOCUS       DAZPAB010000604         1361 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.guided.412, whole genome shotgun sequence.
ACCESSION   DAZPAB010000604 DAZPAB010000000
VERSION     DAZPAB010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.guided.412"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..397
                     /locus_tag="ACHUPG_004616"
     CDS             <1..397
                     /locus_tag="ACHUPG_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFR0863868.1"
                     /translation="NQDFSGDKERNNGLTEAWLESSLKISPEEQIQFLRKIINHNLPV
                     KNSAIENTIENMYLQDLDNSTKLYGKTGAGFTANRTLQNGWFEGFIISKSGHKYVFVS
                     ALTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            510..1348
                     /gene="aadA1"
                     /locus_tag="ACHUPG_004617"
                     /pseudo
     CDS             510..1348
                     /gene="aadA1"
                     /locus_tag="ACHUPG_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT          405 a          317 c          328 g          311 t
ORIGIN      
        1 aaatcaagac ttctctggag ataaagaaag aaacaacgga ttaacagaag catggctcga
       61 aagtagctta aaaatttcac cagaagaaca aattcaattc ctgcgtaaaa ttattaatca
      121 caatctccca gttaaaaact cagccataga aaacaccata gagaacatgt atctacaaga
      181 tctggataat agtacaaaac tgtatgggaa aactggtgca ggattcacag caaatagaac
      241 cttacaaaac ggatggtttg aagggtttat tataagcaaa tcaggacata aatatgtttt
      301 tgtgtccgca cttacaggaa acttggggtc gaatttaaca tcaagcataa aagccaagaa
      361 aaatgcgatc accattctaa acacactaaa tttataaaaa atctaatggc aaaatcgccc
      421 aacccttcaa tcaagtcggg acggccaaaa gcaagctttt ggctcccctc gctggcgctc
      481 ggcgcccctt atttcaaacg ttaaacatca tgagggaagt ggtgatcgcc gaagtatcga
      541 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
      601 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
      661 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
      721 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
      781 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
      841 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
      901 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
      961 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     1021 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     1081 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     1141 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     1201 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgttc
     1261 actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctaaca attcgttcaa
     1321 gccgacgccg cttcgcggcg cggcttaact caagcgttag a
//
LOCUS       DAZPAB010000605         1358 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.guided.413, whole genome shotgun sequence.
ACCESSION   DAZPAB010000605 DAZPAB010000000
VERSION     DAZPAB010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.guided.413"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            <1..397
                     /locus_tag="ACHUPG_004618"
     CDS             <1..397
                     /locus_tag="ACHUPG_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFR0863869.1"
                     /translation="NQDFSGDKERNNGLTEAWLESSLKISPEEQIQFLRKIINHNLPV
                     KNSAIENTIENMYLQDLDNSTKLYGKTGAGFTANRTLQNGWFEGFIISKSGHKYVFVS
                     ALTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            510..1345
                     /gene="aadA1"
                     /locus_tag="ACHUPG_004619"
                     /pseudo
     CDS             510..1345
                     /gene="aadA1"
                     /locus_tag="ACHUPG_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT          402 a          317 c          328 g          311 t
ORIGIN      
        1 aaatcaagac ttctctggag ataaagaaag aaacaacgga ttaacagaag catggctcga
       61 aagtagctta aaaatttcac cagaagaaca aattcaattc ctgcgtaaaa ttattaatca
      121 caatctccca gttaaaaact cagccataga aaacaccata gagaacatgt atctacaaga
      181 tctggataat agtacaaaac tgtatgggaa aactggtgca ggattcacag caaatagaac
      241 cttacaaaac ggatggtttg aagggtttat tataagcaaa tcaggacata aatatgtttt
      301 tgtgtccgca cttacaggaa acttggggtc gaatttaaca tcaagcataa aagccaagaa
      361 aaatgcgatc accattctaa acacactaaa tttataaaaa atctaatggc aaaatcgccc
      421 aacccttcaa tcaagtcggg acggccaaaa gcaagctttt ggctcccctc gctggcgctc
      481 ggcgcccctt atttcaaacg ttaaacatca tgagggaagt ggtgatcgcc gaagtatcga
      541 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
      601 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
      661 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
      721 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
      781 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
      841 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
      901 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
      961 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     1021 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     1081 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     1141 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct aggcaggctt
     1201 atcttggaca agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgttcact
     1261 acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc
     1321 gacgccgctt cgcggcgcgg cttaactcaa gcgttaga
//
LOCUS       DAZPAB010000606         1294 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain M2101073
            SAMN43277595-rid23488913.guided.419, whole genome shotgun sequence.
ACCESSION   DAZPAB010000606 DAZPAB010000000
VERSION     DAZPAB010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277595
            Sequence Read Archive: SRR30464306
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396657.1
            Genome Coverage       :: 53x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:15:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,621
            CDSs (total)                      :: 4,546
            Genes (coding)                    :: 4,071
            CDSs (with protein)               :: 4,071
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 475
            CDSs (without protein)            :: 475
            Pseudo Genes (ambiguous residues) :: 0 of 475
            Pseudo Genes (frameshifted)       :: 228 of 475
            Pseudo Genes (incomplete)         :: 149 of 475
            Pseudo Genes (internal stop)      :: 166 of 475
            Pseudo Genes (multiple problems)  :: 63 of 475
            Pseudo Genes (short protein)      :: 1 of 475
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277595-rid23488913.guided.419"
                     /strain="M2101073"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2021-01-23"
                     /collected_by="FSS"
     gene            94..567
                     /gene="dfrA1"
                     /locus_tag="ACHUPG_004620"
     CDS             94..567
                     /gene="dfrA1"
                     /locus_tag="ACHUPG_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR0863870.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            662..1186
                     /gene="sat2"
                     /locus_tag="ACHUPG_004621"
     CDS             662..1186
                     /gene="sat2"
                     /locus_tag="ACHUPG_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR0863871.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
BASE COUNT          389 a          284 c          297 g          324 t
ORIGIN      
        1 taatacaagt gattatatta attaacggta agcatcagcg ggtgacaaaa cgagcatgct
       61 tactaataaa atgttaacct ctgaggaaga attgtgaaac tatcactaat ggtagctata
      121 tcgaagaatg gagttatcgg gaatggccct gatattccat ggagtgccaa aggtgaacag
      181 ctcctgttta aagctattac ctataaccaa tggctgttgg ttggacgcaa gacttttgaa
      241 tcaatgggag cattacccaa ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct
      301 gacaatgaga acgtattgat ctttccatca attaaagatg ctttaaccaa cctaaagaaa
      361 ataacggatc atgtcattgt ttcaggtggt ggggagatat acaaaagcct gatcgatcaa
      421 gtagatacac tacatatatc tacaatagac atcgagccgg aaggtgatgt ttactttcct
      481 gaaatcccca gcaattttag gccagttttt acccaagact tcgcctctaa cataaattat
      541 agttaccaaa tctggcaaaa gggttaacaa gtggcagcaa cggattcgca aacctgtcac
      601 gccttttgta ccaaaagccg cgccaggttt gcgatccgct gtgccaggcg ttaggcgtca
      661 tatgaagatt tcggtgatcc ctgagcaggt ggcggaaaca ttggatgctg agaaccattt
      721 cattgttcgt gaagtgttcg atgtgcacct atccgaccaa ggctttgaac tatctaccag
      781 aagtgtgagc ccctaccgga aggattacat ctcggatgat gactctgatg aagactctgc
      841 ttgctatggc gcattcatcg accaagagct tgtcgggaag attgaactca actcaacatg
      901 gaacgatcta gcctctatcg aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt
      961 cgcgcacagt ctcatcgaat ttgcgaaaaa gtgggcacta agcagacagc tccttggcat
     1021 acgattagag acacaaacga acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt
     1081 tactctcggc ggcattgacc tgttcacgta taaaactaga cctcaagtct cgaacgaaac
     1141 agcgatgtac tggtactggt tctcgggagc acaggatgac gcctaacaat tcattcaagc
     1201 cgacaccgct tcgcggcgcg gcttaattca ggagttaaac atcatgaggg aagcggtgat
     1261 cgccgaagta tcgactcaac tatcagaggt agtt
//
